LOCUS       DASTNL010000001         6363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1113716, whole genome shotgun sequence.
ACCESSION   DASTNL010000001 DASTNL010000000
VERSION     DASTNL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113716"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VFQ96_00005"
     CDS             <1..1237
                     /locus_tag="VFQ96_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU0256202.1"
                     /translation="RSEVVHDEIISFESEDHDRGIALEVAMQWTTSYSESVYTYANTI
                     NTHEGGTHEEGFRAALTTLVNRTARERGLLKDKDDNLSGDDVREGLTAVVSVKLTEPQ
                     FEGQTKTKLGNTEAKSFVQSVVFKSLADWFDRNPNQTKDIIRKSLQAASARLAARKAR
                     ETARRKGLLESGGMPGKLKDCQSKDPSLSEIFLVEGDSAGGSAVQGRNPITQAILPLR
                     GKILNVEKARLDRALNNAEIQAMITAFGAGIGEDFDPQKARYHKIVLMADADVDGQHI
                     TTLLLTLLFRYMRPMIELGYVYLAQPPLYRLKWSNAPHQYVFSDIEKDALLADGQASG
                     KRMPKDNGVQRYKGLGEMDYKELWETTMSPETRTLLQVTLDDAAAADEVFNTLMGEDV
                     ESRRTFIQQNAKDVRFLDI"
     gene            1320..3848
                     /gene="gyrA"
                     /locus_tag="VFQ96_00010"
     CDS             1320..3848
                     /gene="gyrA"
                     /locus_tag="VFQ96_00010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU0256203.1"
                     /translation="MADETTGTEIGDVDPHGKIDQVDLQLEMQRSYLDYAMSVIVGRA
                     LPEVRDGLKPVHRRVIYAMYDGGYRPDKAFSKCARVVGDVMGQYHPHGDSAIYDALVR
                     LVQPWSLRYPLALGQGNFGSPGNDGAAAPRYTETKMAPLAMEMVRDIDEGTVDFVDNY
                     DGRTQEPYVLPSRFPNLLVNGSVGIAVGMATNIPPHNLREVADGALWSLAHPEATREE
                     LLAALLQRIKGPDFPTGARILGVKGIQEAYRTGRGSITMRAVVNVEEIQGRTCLVVTE
                     LPYQVNPDNLAMKIADLIKDGRIGGISDIRDETSGRTGQRLVIVLKRDAVAKVVLNNL
                     YKHTQLQENFGANMLALVDGVPRTLPIDGFVTAWVNHQVDVIVRRTRFRLEKAEADAH
                     IQRGYVKALDALDDVIALIRRSPTVDEAREGLMGLLAIDQLQADAILTMQLRRLAALE
                     RQKIIDRLAELEEEIAGYRQILADPVRQRAIVAEELTQIVDKYGDDRRTEILHGFDGD
                     MTIEDLIPEEDMVVTLTRGGYVKRTRSDQYRSQHRGGRGVRGAQLRADDIIEHFFVTT
                     THHWLLFFTNFGRVYRAKTYELQEAGRDAKGQHVANFLALQPDEEIAQVLVLRDYDDA
                     KYLVLATRDGLVKKTALSEYDTNRTGGIIAIKLREGDELVSALLAGDDSDILLVSRKG
                     MSLRFTATDEALRPMGRSTSGVIGMHFRGDDSLLDASVLDGGGFVFVVTEGGYAKRTA
                     VDQYRRQGRGGLGIKVAKLTEDRGDLVGAPIVGEDDEVLVVLASGKVVRSAVAEVPAK
                     GRDTMGVVFARFADDDRILALARNNEENLAEDDDAGPTGGLTTQ"
     gene            3848..4249
                     /locus_tag="VFQ96_00015"
     CDS             3848..4249
                     /locus_tag="VFQ96_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEU0256204.1"
                     /translation="MSEAVRAVSGADGASSVRQVRLKLVSVGFWSAVKLSFLIGLCVG
                     IVTLVGAFLVWTMLNQLGIFTKLDTVLTSFVDTGTNLKSIVSLGQVMSFTVIVAILDV
                     VVVTILGAIFAVLYNLSVKITGGLSVGFRNR"
     gene            4529..4602
                     /locus_tag="VFQ96_00020"
     tRNA            4529..4602
                     /locus_tag="VFQ96_00020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4563..4565,aa:Ile,seq:gat)
     gene            4685..4757
                     /locus_tag="VFQ96_00025"
     tRNA            4685..4757
                     /locus_tag="VFQ96_00025"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4718..4720,aa:Ala,seq:tgc)
     gene            complement(4818..4973)
                     /locus_tag="VFQ96_00030"
     CDS             complement(4818..4973)
                     /locus_tag="VFQ96_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256205.1"
                     /translation="MSLGSNPFAAGYQTEVAASPRVGGEAGAGGQASGDDHSPPASVG
                     ERFAEPD"
     gene            complement(5127..5771)
                     /locus_tag="VFQ96_00035"
     CDS             complement(5127..5771)
                     /locus_tag="VFQ96_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256206.1"
                     /translation="MSLKSKRAKELRRLRASAEDLWRDQQVLLQKANVVAREAGRQAG
                     NYTREEVAPRLRDGYEQYVRPRVDDARDLARYAGERIERRVIPAVGTALGTALSVVDV
                     AKDARGKAAAVRRGAIDRVGAVRRVTAVRATRATRRGHRGRTLALCFAAATLTGLAYA
                     VWQTFRADDELWVSEETPAAPSRPTPPATPSAPPTGDDAGKDSSADGGTDTHDA"
     gene            5946..>6363
                     /locus_tag="VFQ96_00040"
     CDS             5946..>6363
                     /locus_tag="VFQ96_00040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0256207.1"
                     /translation="MAQHTAVATLHTNHGDIKVNLYGNHAPKTVRNFVGLATGQTEWT
                     HPGTGKKSKDPLYDGVVFHRIIQGFMIQGGDPLGQGTGGPGYQFDDEINPELDFTEPY
                     VLAMANAGTRGGHGTNGSQFFITVAPTTWLQGKHTIF"
BASE COUNT         1112 a         2094 c         2127 g         1030 t
ORIGIN      
        1 acgttccgag gtcgtgcacg acgagatcat ctcgttcgag tcggaagacc acgaccgcgg
       61 catcgcgctc gaggtggcga tgcagtggac gacctcatac tccgagagcg tctacaccta
      121 cgccaacacc atcaacaccc atgagggggg cacccacgaa gaggggttcc gtgccgcgtt
      181 gacgacgctc gtcaaccgca ccgcgcggga acgtggtctc ctgaaggaca aagacgacaa
      241 tctctcgggc gacgacgtgc gagaaggcct gacggcggtc gtctccgtca agctcaccga
      301 accgcagttc gaaggtcaaa ccaagaccaa gctcggcaac accgaggcga agtcgttcgt
      361 gcaatcggtc gtcttcaaga gcctcgccga ctggttcgac cgcaacccga accagacgaa
      421 agacatcatc cgcaagtcgt tgcaggcagc ctccgcccgg ctcgccgccc gcaaggcgag
      481 ggagaccgcg aggcgcaagg gactcttgga atccggcggc atgcccggca agctgaaaga
      541 ctgccagtcc aaagatccga gcctgtcgga gatcttcctc gtggaggggg attctgcggg
      601 cggctccgcg gtgcaaggcc gcaacccgat cacgcaggcg attctgccgc tgcgcggcaa
      661 gattctgaac gttgagaagg cgcgtctcga ccgagcgttg aacaacgccg agatccaggc
      721 gatgatcacc gccttcgggg cgggcatcgg ggaggatttc gacccgcaga aggctcgtta
      781 ccacaagatc gtgctcatgg ccgacgccga cgtcgacggc cagcacatca cgacgctgct
      841 cctgacactg ctcttccgct atatgcgccc gatgatcgaa ctcggctacg tgtatctcgc
      901 gcagccgccg ctctaccggt tgaagtggag caacgcgccg caccagtacg tgttctccga
      961 catcgagaaa gacgcgcttc tcgccgacgg gcaggcctcc ggaaagcgga tgcccaaaga
     1021 caacggcgtg caacggtaca agggcctcgg cgagatggac tataaggagc tgtgggagac
     1081 cacgatgagc cccgagacgc gcacgctcct ccaggtgacg ctggacgacg cggcggcggc
     1141 cgacgaggtc ttcaacacct tgatgggtga agacgtggag tcgcggcgca ccttcattca
     1201 gcagaacgcg aaagacgtgc gcttcctcga catctgacga gaggccagcg gcacgcacga
     1261 cgatgagggc cggcttcgac cggccgcacg acgaacatcc agcataggac gggcaggcaa
     1321 tggcagacga gacgacgggc acagagatcg gcgacgtcga cccgcacggc aagatcgacc
     1381 aggtcgatct gcagctcgag atgcagcgct cctacctcga ctatgcgatg agcgtcatcg
     1441 tcggtcgcgc tttgccggag gtgcgtgacg gtctgaagcc ggtgcaccgc cgcgtcatct
     1501 acgcgatgta cgacggcgga taccggcccg acaaggcgtt ctcgaagtgt gctcgtgtcg
     1561 tcggcgacgt catgggccaa taccatccgc acggtgacag tgccatctat gacgcgctcg
     1621 tccgtctcgt gcagccgtgg agcctgcgtt acccgctcgc cctcgggcag ggcaatttcg
     1681 gctcccccgg caacgacggg gcggcggcgc cgcgctacac cgagacgaag atggcgccgc
     1741 tggccatgga gatggtgcgc gacatcgacg agggcaccgt cgacttcgtc gacaactatg
     1801 acggccgcac gcaggaaccg tatgtgctgc cgagccggtt ccccaacctg ctcgtcaacg
     1861 gatcggtggg catcgcggtc ggcatggcga cgaatatccc accgcacaat ctgcgcgagg
     1921 tcgccgacgg ggcgctctgg agcctggcgc atccggaggc gacgcgcgaa gagctgctcg
     1981 ccgcccttct tcagcggatc aaggggcccg attttccgac cggggcccgc atcctcggtg
     2041 tgaagggcat tcaggaggcg tatcgcacgg ggcgcggttc catcacgatg cgcgccgtcg
     2101 tgaacgtgga agagattcag ggccgcacct gcctcgtcgt cactgagctg ccctaccagg
     2161 tcaaccccga caacctggcg atgaagatcg cggatctcat caaagacggc cgtatcggcg
     2221 gtatctctga cattcgcgac gagacgagcg ggcgcaccgg ccagcgtctc gtcatcgtgt
     2281 tgaagcgtga cgcggtcgcc aaggtcgtcc tgaacaacct gtacaagcac acccagctgc
     2341 aagagaactt cggcgccaac atgctcgcgc tcgttgacgg cgtgccgcgc accctgccga
     2401 tcgacgggtt cgtcacggcg tgggtgaatc atcaggtcga cgtcatcgtg cgtcgcaccc
     2461 gcttccgcct cgagaaggcg gaggctgacg cgcacatcca gcgcggctac gtgaaggcgc
     2521 tcgacgcgct cgacgacgtc atcgccctca tccgccggtc gccgacggtc gacgaggccc
     2581 gggagggtct catgggcctg ctcgcgatcg accagctgca ggccgacgcg atcttgacga
     2641 tgcagttgcg gcgtctggcc gcgctcgagc ggcagaagat catcgaccgc ctcgccgagc
     2701 tcgaggaaga gatcgccggc taccgccaga ttctcgccga ccccgtccgc cagcgcgcga
     2761 tcgtcgccga agagctcacg cagatcgtcg acaagtacgg cgacgaccgg cgcaccgaga
     2821 tcctgcacgg cttcgacggc gacatgacga tcgaagacct catccccgaa gaagacatgg
     2881 tcgtcacgct cacccggggc ggctatgtca agcgcacgcg cagcgaccag taccgttcgc
     2941 agcaccgtgg aggccgaggc gtgcgcgggg cccagctgcg ggccgacgac atcatcgagc
     3001 atttcttcgt caccacgacc catcactggc tgttgttctt cacgaacttc ggccgtgtct
     3061 atcgcgccaa gacctacgag ctgcaagagg ccgggcgtga cgcgaagggg cagcacgtgg
     3121 cgaacttcct cgccctgcag cccgacgagg aaatcgctca ggtgctcgtg ctgcgcgact
     3181 acgacgacgc gaaatacctg gtgctcgcga cgcgtgacgg cctcgtcaag aagacggcgc
     3241 tgtcggaata cgacacgaat cggacgggcg gcatcatcgc catcaagctg cgggagggcg
     3301 acgagctcgt ctccgccctt ttggcgggcg acgattccga catcctcctc gtctcacgca
     3361 agggcatgtc gttgcgtttc acggccaccg acgaggcgtt gcgcccgatg gggcgctcca
     3421 cctccggcgt gatcggcatg catttccgcg gcgacgacag cctgctcgac gcctccgttc
     3481 tcgacggcgg cggattcgtc ttcgtcgtga cagagggcgg ctacgcgaag cggacggcgg
     3541 tggatcagta ccgccggcag ggccgcggcg gcctcggcat caaagtggcc aaactcaccg
     3601 aagaccgtgg cgatctcgtg ggtgcgccga tcgtcggcga agacgacgag gtgcttgtgg
     3661 ttcttgccag cggcaaggtg gtacgctctg ccgtggccga ggtgcccgcc aaggggcggg
     3721 acacgatggg agtggtgttc gcccgcttcg cggacgacga ccgtatcctc gctctggcca
     3781 ggaataacga ggaaaacctg gcggaggacg acgacgccgg gcccacggga gggcttacca
     3841 cacagtgatg agcgaggcgg tacgcgccgt gagcggcgcc gacggggcga gttcggtcag
     3901 gcaggtgcgg ctgaagctcg tgtcagtcgg tttctggtcg gccgtgaagc tgtcgttctt
     3961 gatcggcttg tgtgtcggta tcgtgacgct cgtcggtgcg ttcctcgtgt ggaccatgct
     4021 gaaccagctg ggtatcttca cgaagctcga taccgtgctc accagcttcg tcgacacggg
     4081 aaccaacctg aagtcgatcg tcagcctcgg ccaggtgatg agcttcacgg tgatcgtcgc
     4141 tattctcgac gtcgtggtcg ttaccatctt gggcgcgatc ttcgccgtgc tgtacaacct
     4201 gagtgtgaag atcaccggag ggctctcggt cggttttcgc aaccgctgac gttcgccgcc
     4261 gttcgtcggc gcgggtgtcg gtagtggttc gggtatggtc gggtctggta gcgcccagct
     4321 cggcgaggca ggggccggtg acgtttccgg tcggcgaggg cgttggcgtt gtgcctttcg
     4381 tcgggggctt gcctgtcgga ggggcgcctt ccgagcttga ttcgcgtcgg agccgtcggt
     4441 cggatattct tgacctgccc gttcgggcgc tcgggccaac gcccggcgcc ggtaaggggc
     4501 gcgccgcgac cggcacggtg gtcggcacgg ggatatagct caggcggtta gagcgcttcg
     4561 ctgataacga agaggtccaa ggttcaagtc cttgtatccc cacggcacgg tgcggcggtc
     4621 accggccggg cacgcgggtg ccgggccagg gcctgctggg tcgtcgtgtc gggttgcggt
     4681 gcacggggtc atagctcagt cggtagagcg cctgctttgc aagcaggagg tccggggttc
     4741 gattccccgt ggctccacac gcgatcgagg tgagaacgcc agaaccgcgt tctcgctgtc
     4801 gggaacggcg ggatcattca gtcgggctcg gcgaagcgct ccccgaccga cgccggaggc
     4861 gagtggtcgt cgcctgacgc ctgaccgccg gcgccggcct ctccgccgac acgagggctc
     4921 gccgcgacct cggtctggta ccccgcggcg aacgggttcg agccaaggga caagccgatc
     4981 cggcacccgc agcgacgtag cgcggagcgc ccgcgcacga tcggcccgac acgcaggcgg
     5041 tcctgacctg gcgcttggaa ataccctggc ccgcaagcct agagcgggca gttggcccgg
     5101 cgtgcggcgc ggcctagcag gcgtccctac gcgtcgtgcg tgtcggtgcc gccgtcggcg
     5161 gaggagtctt taccggcgtc gtcgcccgtg ggcggggcgg acggggttgc gggtggcgtg
     5221 ggccgggagg gtgccgcagg cgtctcttca gagacccaca actcgtcgtc ggcgcggaac
     5281 gtctgccaca ccgcgtaggc caggcccgtc agggtcgcgg cggcgaaaca cagggcgaga
     5341 gtgcggccgc ggtggccgcg ccgggtggct ctggtggctc gcacggcggt gacccgcctt
     5401 accgcgccga ccctgtcgat ggcgccgcgc cgcacggccg ccgccttgcc tcgcgcatcc
     5461 tttgccacgt cgacgacgga taacgccgtg cccagcgccg tgccgaccgc cggaatcacc
     5521 ctgcgttcga tgcgctcacc ggcgtatcgg gcgaggtctc gggcgtcgtc gaccctcggg
     5581 cgcacgtact gttcgtagcc gtcgcgcagc cgcggggcaa cctcttcgcg cgtgtagttg
     5641 ccggcctggc gccccgcctc gcgggcgacg acgttcgcct tctgcagcag aacctgctga
     5701 tcgcgccaca ggtcttcggc gctggctcgc aaccgccgga gttctttcgc gcgcttgctc
     5761 ttcaagctca tccgatcctc ccgtgcgtcg tcaggcgaac gcctccatct tgccaccatc
     5821 gcccgaccgg ccgctgggcg ctcggcgcct tgccggatac ggcgtcggtc cggcgcggca
     5881 ctacgccccc gccataaggc ccctgcggag ccggaggccg aaggcgtcgt gtaagaattg
     5941 aggttatggc acaacacact gcggtcgcga ctctgcacac caaccacggt gacatcaagg
     6001 tcaacctcta cggtaatcac gccccgaaga cggtgcgcaa cttcgtcggc ctcgccaccg
     6061 ggcagaccga gtggacgcac cccggcaccg gcaagaagtc gaaagacccg ctgtacgacg
     6121 gcgtcgtctt tcaccgcatc atccagggct tcatgattca aggcggcgac ccgctcgggc
     6181 agggcaccgg cgggccgggc taccagttcg acgacgagat caaccccgaa ctcgacttca
     6241 ccgagcctta tgtgctcgcc atggcgaacg ccggaacccg cggcggccac ggcaccaacg
     6301 gatcgcagtt cttcatcact gtcgcgccga ccacctggct gcaaggcaag cacaccatct
     6361 tcg
//
LOCUS       DASTNL010000002         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_375718, whole genome shotgun sequence.
ACCESSION   DASTNL010000002 DASTNL010000000
VERSION     DASTNL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375718"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFQ96_00045"
     CDS             <1..534
                     /locus_tag="VFQ96_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEU0256208.1"
                     /translation="AFTIRKRLGGADSRGRGLDGVRVLIVGDILHSRVARSNVWLLTA
                     LGASVTLAGPPTLLPVDTSAWPCEVDYDFDHALSGSPDVIMMLRIQNERMHAAFFPTT
                     REYVHLWGLHEERLARVPRTTMIMHPGPMNRGLEISAAAADSPQSTVREQVANGVSVR
                     MAVLYLLLAGEGGAARA"
     gene            527..1849
                     /locus_tag="VFQ96_00050"
     CDS             527..1849
                     /locus_tag="VFQ96_00050"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0256209.1"
                     /translation="MPDFLVRGVTLVDGSHTDLGISDGFLVPVGDLDRRRQVIDAEGL
                     TALPGLVDLHTHLREPGFEESETVLTGSRAAAAGGFTAVFAMANTFPVADTAGVVEQV
                     ASLGARAGYVTVQPVGAVTVGLKGTRLAEMGAMAASGARVRVFSDDGKCVADPRLMRR
                     ALEYVKAFDGVIAQHAQEPRLTEGAQMNEGRLSARLGLAGWPDVAEEEIVARDVLLAR
                     HVGGRLHVCHVSTAGSVDVIRWAKARGVDVTAEVTPHHLTLTEESAAGYDARFKVNPP
                     LRTDEDVEALRQGLADGTIDVVATDHAPHPAEAKDCEWPVASFGMVGLESALSVVQKA
                     MVDTGLLTWSGLARVLSATPARIGQLAHQGRPVRPGEPANVTLVDPAARRTFTRDDLA
                     GKGVNSAFLGASLPGRVVYTFHRGVETVHEGTLTDLRHTRSASEASHG"
     gene            1842..2444
                     /locus_tag="VFQ96_00055"
     CDS             1842..2444
                     /locus_tag="VFQ96_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256210.1"
                     /translation="MGETVSVIVVTAAFAACVVWMVVAWRHRLHGETGLLPALTEPGM
                     RGAERLFLSDLGYVVTTLHDKPFERLAIGPLAYPGRADLAVHDGGIALRLVGADEVFV
                     PAASIVSIGKASWALGRAVEKDGMVVVEWICGDGRTRADSYLRVLRPGDDLLVVRAIA
                     AAAEAALPGGAWRHEKVLGDAGDAPNAGNNEKEIREHQHG"
     gene            2398..>2576
                     /locus_tag="VFQ96_00060"
     CDS             2398..>2576
                     /locus_tag="VFQ96_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEU0256211.1"
                     /translation="MPVTTRRRSVSISTAETTSAVLVLEDGTRYEGRAYGALGKTLGE
                     AVFATGMTGYQETLT"
BASE COUNT          364 a          909 c          911 g          392 t
ORIGIN      
        1 gcgttcacga tccgcaagcg cctgggcggc gccgactccc gcggccgagg tctcgacggg
       61 gtgcgcgtgc tcatcgtcgg cgacattctg cactcccgcg tggcgcgctc gaacgtctgg
      121 ctcttgaccg cgctcggggc gagcgtcacg ctcgccggcc cgccgacgct cctgcccgtc
      181 gacacgagcg cgtggccgtg cgaggtcgac tacgacttcg accacgccct gtcgggctcg
      241 cccgacgtga tcatgatgtt gcgcatccag aacgaacgga tgcacgccgc gttcttcccc
      301 acgacgcgcg agtatgtgca tctctggggg ctgcacgagg aaaggctggc acgcgtgccc
      361 cgaacgacca tgatcatgca tcccggcccg atgaaccggg gcctggagat ctctgcggcc
      421 gccgcggatt ccccgcagtc gacggtgcgg gagcaagtgg ccaacggggt cagcgtgcgg
      481 atggcggtgc tgtacctgct cctggcagga gaggggggtg cggcccgtgc ctgactttct
      541 cgtgcgcggc gtcacgctgg tcgacggctc ccacaccgat ctgggaattt ccgacgggtt
      601 tctcgtgcct gtcggcgacc tcgaccggcg ccggcaggtc atcgacgccg aaggccttac
      661 cgccctgccc ggcctcgtcg acctgcacac gcatctgcgg gagcccgggt tcgaagagag
      721 cgagacggtt ctgaccggca gtcgggccgc ggccgcgggt ggcttcacgg cggtcttcgc
      781 gatggcgaac accttccccg tcgccgacac ggcaggcgtc gtcgaacagg tggcctcgct
      841 cggcgcgcgg gccggctacg tcaccgtgca gccggtcggt gcggtcacgg tcggcctgaa
      901 gggcacgcgc ctcgccgaga tgggcgcgat ggccgcgtcg ggcgcccgcg tgcgggtctt
      961 ctccgatgac ggcaaatgtg tcgccgaccc gcgtctcatg cgtcgtgccc tcgaatacgt
     1021 gaaggcgttc gacggcgtca tcgcccagca cgcgcaagag ccgcgcctca ccgagggcgc
     1081 ccagatgaac gagggccggc tttccgcccg cctcggcctc gccggctggc ccgacgtggc
     1141 ggaggaggag atcgtcgccc gcgacgtgct cctcgcccgc catgtcggag gccggctgca
     1201 cgtctgccac gtctccaccg ccggcagcgt cgacgtcatc cggtgggcga aggcgcgcgg
     1261 cgtggacgtg acggccgagg tcacgccgca tcacctgacg ctcaccgagg aatccgccgc
     1321 cggctacgac gcgcgcttca aagtgaaccc gccgctgcgc accgacgagg acgtcgaggc
     1381 gttgcgccag ggcttggccg acggcaccat cgacgtcgtg gcgaccgacc acgcgcccca
     1441 tcctgccgag gcgaaagact gcgaatggcc cgtcgcctcg ttcggcatgg tggggctgga
     1501 atcggcgttg agcgtcgtgc agaaagcgat ggtcgacacg ggccttctga cctggtccgg
     1561 actcgcgcgg gtcttgtcgg ccaccccggc gcggatcggc cagctggcgc accagggccg
     1621 gccggtccgg ccgggcgagc cggccaacgt gacgcttgtc gaccccgccg ccaggcgcac
     1681 cttcactcgc gacgacctcg cgggcaaagg cgtcaactcc gccttcctcg gcgccagcct
     1741 gccgggtcgt gtcgtgtata ccttccatcg tggcgtcgag accgtgcacg agggcacgct
     1801 caccgacctg cgccacaccc gttcggcatc ggaggcctcg catgggtgaa actgtttcgg
     1861 tcatcgtcgt caccgccgcg ttcgcggcct gcgtcgtctg gatggtcgtg gcgtggcgtc
     1921 atcgcctgca tggcgagacg ggcctcctgc ccgcactgac ggagccgggg atgcgcggcg
     1981 cggaacgact tttcctcagc gacctcggct acgtcgtgac caccctgcat gacaagcctt
     2041 tcgaacgcct cgcgatcggc cctctcgcct accccggccg ggccgacctg gccgtccacg
     2101 acgggggcat cgctctgcgc ctcgtcggcg ccgacgaggt cttcgtgccc gccgcctcga
     2161 tcgtctcgat cggcaaggcg agttgggcgc tcggccgcgc cgtggagaag gacggcatgg
     2221 tggtcgtcga atggatctgc ggcgacgggc gcacccgggc cgacagctac ctgcgcgtgt
     2281 tgcgcccggg cgacgacctg ctcgtcgtgc gggcgatcgc ggccgcggca gaggccgccc
     2341 tgccgggcgg cgcgtggcgc cacgagaagg ttctgggcga cgccggcgac gcgccgaatg
     2401 ccggtaacaa cgagaaggag atccgtgagc atcagcacgg ctgaaacgac atcggcggtc
     2461 ctcgtcttag aagacgggac gcgctacgag ggccgagcat acggggcgct cgggaagacg
     2521 ctgggcgagg ccgttttcgc gacggggatg accggttacc aggagaccct caccga
//
LOCUS       DASTNL010000003         9128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_931618, whole genome shotgun sequence.
ACCESSION   DASTNL010000003 DASTNL010000000
VERSION     DASTNL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931618"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..690
                     /gene="rpsJ"
                     /locus_tag="VFQ96_00065"
     CDS             382..690
                     /gene="rpsJ"
                     /locus_tag="VFQ96_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0256212.1"
                     /translation="MAGQKIRIRLKSYDHEVIDTSAGKIVDTVTRAGATVVGPVPLPT
                     EKNVVCVIRSPHKYKDSREHFEKRTHKRLIDIIDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            713..1369
                     /gene="rplC"
                     /locus_tag="VFQ96_00070"
     CDS             713..1369
                     /gene="rplC"
                     /locus_tag="VFQ96_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU0256213.1"
                     /translation="MTERLIRTTKGLLGTKLGMTQVWDENNRVVPVTVIEVGTNVVTQ
                     LRTPETDGYSAVQIAYGQVDPRRVDKPTTGHFAKAGLPPRRHLAEVRTDDASNYTVGQ
                     ELTAGLFTVGELVDVVGTSKGKGFAGTIKRHNFGGVSASHGAHRNHRKPGSIGASSTP
                     SRVFKGWRMAGRMGGNRVTVLNLTLQAVDAEKGLLLVKGAVPGARGRLVFVRNAIKGA
                     "
     gene            1374..2030
                     /gene="rplD"
                     /locus_tag="VFQ96_00075"
     CDS             1374..2030
                     /gene="rplD"
                     /locus_tag="VFQ96_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU0256214.1"
                     /translation="MAATIDVVDVQGNKSGAVQLPEALFDTQTNVPLIHQVVVAQLAA
                     ARQGTHKVKTRGEVSGAGRKPFKQKGTGNARQGSIREPQMTGGGIVHGPMPRSYAQRT
                     PKKMIAAALFGALSDRARGGRIHVVEKLNAGNTPSTKAAAATLAAVADSARVLVVLGR
                     GEEVDYRSVRNLPQVHTLTFGQLNAYDVLVADDIVFTKAAFDGFVATRAATPTKEAEK
                     "
     gene            2027..2326
                     /gene="rplW"
                     /locus_tag="VFQ96_00080"
     CDS             2027..2326
                     /gene="rplW"
                     /locus_tag="VFQ96_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU0256215.1"
                     /translation="MSAVNKDPREIILAPVVSEKSYGLIDEGKYTFVVDRRANKTEVK
                     LAIEKIFSVKVAAVNTLNRRGKKQRTRFGIGRRKDTKRAIVTLKSGTIDIFTSVG"
     gene            2350..3189
                     /gene="rplB"
                     /locus_tag="VFQ96_00085"
     CDS             2350..3189
                     /gene="rplB"
                     /locus_tag="VFQ96_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU0256216.1"
                     /translation="MAIRKYKPTTPGRRGSSVADFAEITRSRPEKSLVRPLPKTGGRN
                     NQGRITTRHIGGGHKRQYRLIDFRRSDKDGVNAKVAHIEYDPNRTARIALLHFVDGTK
                     RYILAPDGLRQGDVVESGSGADIKPGNNLPLRNIPTGTVVHAIELKPGGGAKLARSAG
                     TSVRLVAKEGPYAQLRLPSGEIRNVDARCRASIGEVGNAEQSNINWGKAGRSRWKGVR
                     PTVRGVVMNPVDHPHGGGEGRTSGGRHPVSPWGQPEGRTRHPNKESDKLIVRRRPTGK
                     KRK"
     gene            3211..3492
                     /gene="rpsS"
                     /locus_tag="VFQ96_00090"
     CDS             3211..3492
                     /gene="rpsS"
                     /locus_tag="VFQ96_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU0256217.1"
                     /translation="MPRSLKKGPFVDEHLLRKVVAANEAKSKNVIKTWSRRSMVVPAM
                     LGHTIAVHDGRRHIPVFITEAMVGHKLGEFAPTRTFRGHVKDDKKGRRR"
     gene            3516..3881
                     /gene="rplV"
                     /locus_tag="VFQ96_00095"
     CDS             3516..3881
                     /gene="rplV"
                     /locus_tag="VFQ96_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU0256218.1"
                     /translation="MVESIARLRHIRVTPQKARRVVNMIRGRKAQEALAILKFAQQGA
                     SEPLYKLVASAIANAHVKADAANAYLDESTLYVRKIYVDEGQTLKRFQPRAQGRAFKV
                     LKRTSHITVELATPDEGTK"
     gene            3881..4648
                     /gene="rpsC"
                     /locus_tag="VFQ96_00100"
     CDS             3881..4648
                     /gene="rpsC"
                     /locus_tag="VFQ96_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU0256219.1"
                     /translation="MGQKINPYGFRLGITTDHRSRWFADSTKPGQRYADYVAEDVRIR
                     QMLQKSLDRAGISRIDLERTKDRVRVDIHTARPGIVIGRRGVEAERIRSELEKLTGKQ
                     IQLNILEVKNPEADAQLVAQGVAEQLSARVAFRRAMRKGLQGAQRAGAKGVRIQVSGR
                     LGGAEMSRSEFYRDGRVPLHTLRANIDYGFYEARTTFGRIGVKVWIYKGDITNKELAR
                     EQASRRSGRPERQGGERRGRSAAPQADAAPVSEGATA"
     gene            4651..5070
                     /gene="rplP"
                     /locus_tag="VFQ96_00105"
     CDS             4651..5070
                     /gene="rplP"
                     /locus_tag="VFQ96_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU0256220.1"
                     /translation="MLIPRRVKYRKQHHPRRRGQASGGTRVVFGDYGIQALTGAYVTN
                     RQIESARIAMTRYIKRGGKVWINVYPDRPITKKPAETRMGSGKGSPEWWVASVKPGRV
                     LFELSGVGEDVAREALTRAIHKLPLKARIIQREEGDA"
     gene            5070..5393
                     /gene="rpmC"
                     /locus_tag="VFQ96_00110"
     CDS             5070..5393
                     /gene="rpmC"
                     /locus_tag="VFQ96_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU0256221.1"
                     /translation="MIGSKELAPDELETFDNERLTDELKKAREELFNLRFQAATGQLE
                     SHGRIRAVKRDIARIYTVIRERELGIRATPTPAEAKPKRSKKADKAAAEEKAVPADEA
                     ETKEA"
     gene            5397..5678
                     /gene="rpsQ"
                     /locus_tag="VFQ96_00115"
     CDS             5397..5678
                     /gene="rpsQ"
                     /locus_tag="VFQ96_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU0256222.1"
                     /translation="MAVTEEAAPAARGYRKVRRGYVVGDKMDKTITVEVEDRKRHALY
                     GKIIRRTSKVKVHDAQNSAGVGDLVVISETRPLSATKRWRLVEILEKAK"
     gene            5728..6096
                     /gene="rplN"
                     /locus_tag="VFQ96_00120"
     CDS             5728..6096
                     /gene="rplN"
                     /locus_tag="VFQ96_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU0256223.1"
                     /translation="MLQQESRVKVADNTGTKELLTIRVLGGSKRRYGGLGDIIVATVK
                     DAIPGGNVKRGDVVKAVIVRTVKPTRRADGSYIRFDENAAVLLKSDGDPRGTRIFGPV
                     GRELRDRKFMKIISLAPEVI"
     gene            6101..6463
                     /gene="rplX"
                     /locus_tag="VFQ96_00125"
     CDS             6101..6463
                     /gene="rplX"
                     /locus_tag="VFQ96_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU0256224.1"
                     /translation="MANKIKKGDLVQVITGATQARGGDRGKQGRVIQVLPGRNRVIVE
                     GIHFVTRHTKLGQSQGGTKTGGIEKVEAPIHVSNIALVDPETKKPTKVGFRIETVVKD
                     GVAKTVRTRYAKKSGKDL"
     gene            6464..7051
                     /gene="rplE"
                     /locus_tag="VFQ96_00130"
     CDS             6464..7051
                     /gene="rplE"
                     /locus_tag="VFQ96_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU0256225.1"
                     /translation="MSDTATTTAPQPRLKKRYADEIVAQLTKDFGYTNVHQVPGLVKI
                     IVNTGVGDAARDSKVMDGAVNDLALITGQKPQVTKARKSIAQFKLREGQPIGAHVTLR
                     GNRAWEFLDRLLSLALPRIRDFRGLSDRQFDGRGNYTFGLTEQSVFHEIDQDKIDRVR
                     GFGITVVTSAKTDDEGRALLRALGFPFTSNTPNAG"
     gene            7162..7560
                     /gene="rpsH"
                     /locus_tag="VFQ96_00135"
     CDS             7162..7560
                     /gene="rpsH"
                     /locus_tag="VFQ96_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU0256226.1"
                     /translation="MTMTDPVADMLTRLRNANTAYHDTVAMPHSKLKANIAGILKTEG
                     YIADWAVSDARVGKTLTLTLKFGPNRERSISGIRRVSKPGLRVYAKSTELPKVLGGLG
                     VALLSTSSGLLTDRQAEQKGVGGEVLAYVW"
     gene            7567..8103
                     /gene="rplF"
                     /locus_tag="VFQ96_00140"
     CDS             7567..8103
                     /gene="rplF"
                     /locus_tag="VFQ96_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU0256227.1"
                     /translation="MSRIGRLPIDIPAGVQVKLDGRTVTVTGPKGELTLAVASPIEVA
                     VAEGQVLVTRPDDERESRALHGLTRTLIHNNILGVTQGYTKGLEIVGTGYRVAQKGAS
                     VEFALGFSHPVVVEPPAGITLTVEGNTKLTVSGIDKQAVGEAAANIRKIRKPEPYKGK
                     GIRYAGEVVRRKAGKAGK"
     gene            8104..8472
                     /gene="rplR"
                     /locus_tag="VFQ96_00145"
     CDS             8104..8472
                     /gene="rplR"
                     /locus_tag="VFQ96_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU0256228.1"
                     /translation="MSNNRGTSRAAARGRRHARLRKKVQGTNSRPRLVVTRSSRHVFV
                     QVIDDGAGRTLASGSTMEAGLRSFEGDKTAKAHRVGELVAERAKAAGIADVVFDRGGA
                     KYAGRVAAVAEGAREGGLNL"
     gene            8469..>9128
                     /gene="rpsE"
                     /locus_tag="VFQ96_00150"
     CDS             8469..>9128
                     /gene="rpsE"
                     /locus_tag="VFQ96_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU0256229.1"
                     /translation="MSTETNKEQNVSDTSTSTEAGASAQGGQRDSRERDGRRGGGRER
                     GGRDRRGGGGRGGRDDRNQFLERVVTINRVSKVVKGGRRFSFTALVVVGDGDGTVGVG
                     YGKAREVPLAISKGVEEAKKNFFRVPRLEKTIPHPVQGEAAAGVVLLRPAAAGTGVIA
                     GGPVRAVLECAGIHDVLSKSLGSSNTINIVHATIAALHQLEEPRTVAARRGLDYEAVA
                     PA"
BASE COUNT         1791 a         3035 c         3047 g         1255 t
ORIGIN      
        1 gacgcgacac gcccgggttg cacggttcct tgcgatgtgg cacactcgtc tagttcgaca
       61 tttcgcgcgc gtcgcatcgc gtagcgcgcg gatcactgcc aggcagtgca tagccggccc
      121 gcgagggtgc ggttaggccg cgggcagagg gcacgccccc atatcgattc atacccttgc
      181 ggggcaccgg ctcattcgac ggacgcgatc gctcgcgtcc gcgccgcgag gcgcgcgtcg
      241 atcggccggc cgggcgcgcg agtggtcgcg cccggcattg atagaaaaac agcggtgaga
      301 acgcggcgcg agccgccgaa cgccacgcgc gtccaatgcg gccttggccg tatgacgcct
      361 gagaaagaga gcgagcccga catggcggga cagaagatcc gcattcgcct gaagtcgtac
      421 gaccatgagg tcattgacac ctcggcggga aagatcgtcg acaccgtcac ccgtgccggt
      481 gccacggtgg tcggcccggt gccgttgccc accgagaaga acgtggtctg cgtcatccgc
      541 tcgccgcaca aatataagga cagccgcgag cacttcgaga agcgcacgca caagcggctc
      601 atcgacatca tcgacccgac cccgaaggcc gtcgactcgt tgatgcgcct cgaccttccg
      661 gccgacgtca acatcgagat caagctctga cgggccgaag gcatacagaa ccatgactga
      721 gagactgatc agaaccacga agggcctgct gggcacgaag ctcggcatga cccaggtgtg
      781 ggacgagaac aaccgcgtcg tgcccgtcac cgtcatcgag gtcggcacca acgtcgtcac
      841 gcagctgcgc acgcccgaga ccgacggcta cagcgccgtg cagatcgcat acggccaggt
      901 cgacccgcgc cgcgtcgaca agcccaccac cggccacttc gcgaaggcgg ggctaccgcc
      961 gcgccggcat ctcgccgagg tgcgcaccga cgacgcgtcg aactacacgg tgggccaaga
     1021 gctgacggca ggcctcttca ccgtcgggga actcgtcgac gtcgtcggaa cctcgaaggg
     1081 caaggggttc gcgggcacga tcaagcgcca caacttcggc ggcgtctccg cctcgcacgg
     1141 cgcgcaccgc aaccaccgca agccgggctc gatcggcgcc tcctcgacgc ccagccgcgt
     1201 cttcaaggga tggcgcatgg ccggccgcat gggcggcaac cgggtcaccg tcttgaacct
     1261 gacccttcag gcggtcgacg ccgagaaggg ccttctcctc gtcaagggtg ccgttcccgg
     1321 cgcccgcggc cgtctcgtct tcgtccgcaa cgcaatcaag ggagcgtgag gcaatggctg
     1381 ccacaatcga cgtcgtcgac gtgcaaggca acaagtcggg tgccgtccag ctgcccgagg
     1441 cgctcttcga cacgcagacc aacgttccgc tgatccacca ggtcgtcgtg gctcagctgg
     1501 ccgccgcccg gcagggcacc cacaaggtca agacccgtgg ggaggtctcc ggcgcggggc
     1561 gcaagccttt caagcagaag ggcaccggaa acgcccggca gggctccatc cgcgagccgc
     1621 agatgaccgg cggcggtatc gtgcacggcc cgatgccgcg cagctatgcg caacgaacgc
     1681 ccaagaagat gatcgcagct gcgctgttcg gcgcactctc cgaccgtgcc cggggcggcc
     1741 gcatccacgt cgtcgagaaa ttgaacgccg gcaacacccc gtcgacgaag gctgctgcgg
     1801 ccactctggc cgccgtcgcc gactccgccc gggtgctcgt cgtgctgggc cgcggcgaag
     1861 aggtcgacta tcgcagcgtg cgtaacctcc cgcaggttca caccctcacc ttcggccagt
     1921 tgaacgccta cgacgtgctc gtggccgacg acatcgtctt caccaaggcg gcgtttgacg
     1981 ggttcgtcgc cacgcgtgcc gcgaccccga ccaaggaggc cgagaaatga gcgccgtcaa
     2041 caaggacccg cgcgagatca tcctggcgcc cgtcgtctcc gagaagagct acggcctgat
     2101 cgacgagggc aaatacacgt tcgtcgtcga ccggcgagcc aacaagaccg aggtcaagct
     2161 cgcgatcgag aagatcttca gtgtcaaggt ggccgccgtc aacacgctga accggcgggg
     2221 caagaagcaa cgcacccggt tcggcatcgg ccgccgcaag gacacgaagc gggcgatcgt
     2281 cacgctgaag tccggcacga tcgacatctt cacgtccgtc ggctgatcga gtgtaggtag
     2341 aggaaaacga tggcaattcg caaatacaag ccgacgaccc ccggtcggcg cggctcgtcc
     2401 gttgccgact tcgcggagat cacccgctcc cgccccgaga agtcgctcgt gcggccgttg
     2461 ccgaagaccg gcgggcgcaa caaccagggt cgcatcacga cccggcacat cggcggtggg
     2521 cacaagcgtc agtaccggct gatcgacttt cgtcgcagcg acaaagacgg cgtcaacgcc
     2581 aaggtcgcgc acatcgaata cgacccgaac cggacggcgc gcatcgcgct actgcatttc
     2641 gtcgacggca cgaagcgcta catcctcgcc cccgacggcc tgcggcaggg tgacgtggtg
     2701 gagtcgggtt cgggcgccga catcaagccg ggcaacaacc tgcccttgcg caacatcccc
     2761 accggcaccg tcgtgcacgc tatcgagctg aagccgggcg gcggcgcgaa gctcgcccgt
     2821 tcggccggca cctcggtgcg gctcgtggcg aaagagggcc cgtacgcgca gttgcgcctg
     2881 ccctccggtg agatccgcaa cgtcgacgcc cgttgccgtg cctctatcgg cgaggtgggc
     2941 aatgccgagc agtcgaacat caactggggc aaggcgggcc gcagccgctg gaagggcgtt
     3001 cgccccacgg tgcgcggcgt cgtcatgaat ccggtggacc acccgcacgg cggcggcgag
     3061 ggccgcacgt cgggcggccg tcacccggtg agcccctggg gccagcccga aggccgcacg
     3121 cgccatccga acaaagagag cgacaagctc attgttcgtc gccggcccac cggcaagaag
     3181 cgcaagtagg cgagggaaaa ggaacgcacg atgccacgca gtcttaaaaa aggccccttc
     3241 gtcgacgagc acctgcttcg caaggtcgtc gcggcgaacg aggcgaagag caagaacgtg
     3301 atcaagacct ggtcgcgccg ttcgatggtg gtgccggcga tgctcggcca cacgatcgcg
     3361 gtgcacgacg ggcgccggca catcccggtg ttcatcacgg aggcgatggt cggccacaag
     3421 ctgggcgagt tcgcgccgac ccggaccttc cgcggtcacg tgaaagacga caagaagggc
     3481 cgtcgccgct gacgcggcga cgtaaggagg aagaaatggt ggagtcgatc gccaggttgc
     3541 gacacatccg cgtgacccct cagaaggccc gtcgcgtcgt caacatgatc cgtggcagga
     3601 aggcgcaaga ggcgctggcc attctgaagt tcgcgcagca aggcgccagc gagcccctgt
     3661 acaagctggt cgcctcggcg atcgcgaatg cccacgtgaa ggccgacgcg gcgaacgcct
     3721 atctcgacga gagcacgctc tacgtgcgca agatctacgt cgacgaaggg cagacgctca
     3781 agcgtttcca gccccgggca cagggccgtg cgttcaaagt cttgaagcgc acgagtcata
     3841 tcaccgtcga actggcaacg cctgacgagg ggacgaagta atgggccaga agatcaatcc
     3901 ttacggcttc cggctcggca tcaccaccga tcaccgttcc cgctggtttg ccgacagcac
     3961 gaagccgggg cagcgctacg ccgactatgt ggcagaagac gtgcgcatcc gccagatgct
     4021 gcagaagagc ctcgataggg ccggcatctc ccgcatcgac ctggagcgca ccaaagaccg
     4081 ggtccgcgtc gacatccaca cggcgcggcc gggcatcgtc atcggccgcc ggggcgtcga
     4141 ggcggagcgc attcgcagcg agctcgagaa gctgaccggc aagcagattc agttgaacat
     4201 tctcgaggtg aagaacccgg aggccgacgc tcagttggtc gcccagggcg tggcggagca
     4261 gttgagcgcc cgcgtcgcct tccgccgcgc gatgcgcaag ggtttgcaag gcgcgcagcg
     4321 cgccggggcc aagggcgtgc gcatccaggt ctccgggcgc ctcggcgggg cggagatgag
     4381 ccgcagcgag ttctaccgtg acggccgcgt gccgttgcac accctgcggg ccaacatcga
     4441 ctacggcttc tacgaggccc gcacgacctt cggccgcatc ggtgtgaagg tatggatcta
     4501 caagggcgat atcacgaaca aggagcttgc ccgcgagcag gcgagccgcc gctccggcag
     4561 gcccgagcgg cagggcgggg agcgccgcgg ccgttcagcg gcaccgcagg ccgatgccgc
     4621 gcccgtctca gaaggagcca cggcgtaatc atgctgatcc cacgcagagt caagtaccgt
     4681 aagcagcacc acccgcgccg ccgcgggcag gcgtcgggcg gcacccgcgt cgtcttcggc
     4741 gactacggca ttcaggcgct caccggcgcc tacgtcacca accggcagat agagtcggct
     4801 cgcatcgcga tgacccgcta catcaagcgg ggcggcaagg tgtggatcaa cgtctatcct
     4861 gaccgcccca tcaccaagaa gccggccgag acccgtatgg gtagcggcaa gggctccccg
     4921 gaatggtggg tcgcgagcgt caagcccggc cgggtgttgt tcgagctgag cggtgtcggc
     4981 gaggacgtcg cacgcgaggc gctcacccgc gccatccaca agctgccctt gaaggcacgg
     5041 atcatccagc gtgaggaagg cgacgcgtaa tgatcggttc taaggaactt gctcccgacg
     5101 agctggagac cttcgacaac gaacgtctca ccgatgaatt gaagaaggcc agggaagagc
     5161 tcttcaacct acgcttccag gcggcgaccg gccagctgga gagccacggc cgcatccgtg
     5221 ccgtcaagcg cgacatcgcc cgcatctaca ccgtgatcag ggaacgcgag ctcggcatcc
     5281 gcgccacgcc gacgccggcc gaggcgaagc ccaagagaag caagaaggcc gacaaggccg
     5341 cggctgaaga gaaggccgtg cccgccgacg aggcagaaac gaaggaggcc tagctcatgg
     5401 ctgttaccga agaggcggcg cccgcggccc gcggctaccg caaggttcgt cgtggctacg
     5461 tcgtcggcga caagatggac aagaccatca cggtcgaggt cgaagaccgc aagaggcacg
     5521 cgttgtacgg caagatcatc cgccgcacct cgaaagtcaa ggtgcacgac gcccagaact
     5581 ccgccggcgt cggcgatctc gtcgtgatca gcgagacacg gccgctcagc gccaccaagc
     5641 gctggcgtct cgtcgagatt ctggagaagg cgaagtaggc gccgcacccg gaacgggacg
     5701 acacccactt cgatagaagg acacaacatg cttcagcagg aatcacgagt caaggtggcc
     5761 gacaacaccg gcaccaagga gctcctgacc atccgcgttc tcggcggctc gaaacgccgt
     5821 tacggcgggc tcggcgacat catcgtcgcc acggtcaaag atgcgatccc cggcggcaac
     5881 gtcaaacgcg gcgatgtcgt gaaggcggtc atcgtgcgca ccgtcaagcc gacgaggcgc
     5941 gccgacggct cctatatcag gttcgacgag aacgcggcgg tcctcttgaa gagcgacgga
     6001 gacccgcgcg gcacccgcat cttcgggccg gtcggccggg aattgcggga tcggaagttc
     6061 atgaagatca tctcgctggc accggaggtt atctaaggcc atggccaata agatcaagaa
     6121 gggcgatctc gtccaggtca tcaccggcgc gactcaggcg cgtggcgggg atcgaggcaa
     6181 gcagggtcgt gtgatccagg tgctgccggg ccggaaccgt gtcatcgtcg agggcatcca
     6241 cttcgtcaca cggcacacca agctcggcca gtcgcagggc ggcacgaaga ccggtgggat
     6301 cgagaaggtg gaggcaccca tccatgtctc caacatcgcc ctcgtcgacc ccgagacgaa
     6361 gaagccgacg aaggtcggct tccgcatcga gaccgtcgtg aaagacggcg tcgcgaagac
     6421 ggttcgcacc cgctacgcga aaaagtcagg taaggacctg taaatgagcg acacggccac
     6481 gacgacggcg ccccagccgc gcctgaagaa gagatacgcc gacgagatcg tcgcgcagtt
     6541 gacgaaggac ttcggctaca cgaacgtgca ccaggtgccg ggcctcgtga agatcatcgt
     6601 caacacgggc gtgggcgacg cggcgcgcga cagcaaggtg atggatggcg cggtcaacga
     6661 cctcgccctc atcaccggtc agaagcctca ggtgacgaag gcccgcaagt ccattgcgca
     6721 gttcaagctg cgggagggcc agccgatcgg ggcgcatgtc acgttgcgcg gcaaccgggc
     6781 gtgggagttc ctcgaccgtc tgctctcgct cgccctgccc cgcatccgtg acttccgcgg
     6841 gctgagcgac cggcagttcg acgggcgagg caactacacg ttcggcctga ccgagcaaag
     6901 cgtgttccac gagatcgatc aagacaagat cgaccgggtg cgcggcttcg gcatcacggt
     6961 cgtcacgagc gcgaagaccg acgacgaggg ccgggcgctt ctgcgagcac tcggattccc
     7021 cttcacctcg aacaccccga acgcgggcta ggggccgtgc acgacggccg gtcgcggggc
     7081 aagcctccgg ccgggcgaga tttatcacag tcggcactct tgtaaaggct gtcgaaacct
     7141 gatgaacgaa ggaaaccatt catgacaatg acagatccgg tcgcggacat gctgaccaga
     7201 ctgcgcaacg cgaacacggc ctaccacgac accgtggcca tgcctcattc gaagctcaag
     7261 gcgaacatcg ccggcatctt gaagaccgaa ggatacatcg ccgactgggc cgtcagtgac
     7321 gcccgcgtcg gcaagacctt gacgctcacg ctcaagttcg gcccgaaccg cgagcgttcc
     7381 atctcgggca ttcggcgcgt ttcgaagccg gggctgcggg tgtatgcgaa gtcgacggag
     7441 ttgccgaagg tgctcggcgg gctcggcgtc gcgctgctct ccacctcgtc ggggctcctc
     7501 accgaccggc aagccgaaca gaagggcgtg ggtggggaag tcctcgccta cgtgtggtga
     7561 tccgtcatgt cacgtatcgg aagacttccc atcgacattc ccgccggcgt tcaggtgaag
     7621 ctcgacgggc gcacggtcac cgtcacgggg cccaagggtg agttgacgct cgccgtcgcg
     7681 agcccgatcg aggtcgcggt cgccgagggc caggtgctcg tcacccgccc cgacgacgag
     7741 cgcgagtccc gcgccttgca cggcctcacc cgcaccctca tccacaacaa catcctgggc
     7801 gtcacgcagg gctataccaa aggccttgag atcgtcggta cgggctaccg cgtcgcccag
     7861 aagggcgcct cggtcgagtt cgcgctcggc ttctcgcacc ccgtggtcgt cgagccgccc
     7921 gccggcatca ccctgaccgt cgagggcaac acgaagctca ccgtcagcgg catcgacaag
     7981 caggcggtgg gtgaggccgc ggcgaacatt cgcaagatcc gcaaaccgga gccgtacaag
     8041 ggcaagggca tccgatacgc cggcgaggtc gtgcgtcgca aggccggaaa ggctggtaag
     8101 taagtgtcca acaacagagg aaccagcagg gccgcggcgc gcgggcgtcg gcacgcccgg
     8161 ctgcgcaaga aggttcaggg gaccaactcc aggccacgtc tggtcgtcac ccgctccagt
     8221 cgacacgtct tcgtgcaagt catcgacgac ggcgccgggc gcaccctggc ctccgggtcg
     8281 acgatggagg ccggactgcg ctcttttgag ggtgacaaga ccgcgaaggc gcaccgcgta
     8341 ggcgaactcg tcgccgagcg tgccaaggcc gccggcatcg cagacgtcgt cttcgatcgc
     8401 ggcggggcga aatatgcggg ccgcgtcgcg gccgtcgcgg agggcgccag ggaagggggc
     8461 ctgaacctgt gagcaccgag acgaacaagg aacagaacgt gtccgatacg agcacttcca
     8521 ccgaggccgg cgcgtcggcg cagggcggcc agcgtgacag ccgcgaacgt gacgggcgtc
     8581 gtggcggcgg ccgggagcgg ggcggccgcg atcgtcgtgg cggcggcggc cggggcggcc
     8641 gcgacgaccg caaccagttc ttggagcgcg tcgtgacgat caaccgcgtc tccaaggttg
     8701 tgaagggcgg ccggcgcttc agcttcacgg ccctcgtcgt cgtcggcgac ggcgacggca
     8761 ccgtcggggt cggctacggc aaggcccgcg aggtgccgct ggcgatctcg aagggggtcg
     8821 aagaggcgaa gaagaacttc ttccgcgttc cgcgcctcga gaagacgatc ccgcacccgg
     8881 tgcaaggcga ggcggcggcg ggcgtcgtgc ttcttcggcc ggcggccgcg ggaaccggcg
     8941 tcatcgccgg cggcccggtt cgtgccgtcc tggagtgcgc cggcatccac gacgtgctca
     9001 gcaagtcgct gggctcgtcg aacaccatca atattgtgca cgccacgatc gccgcgctgc
     9061 accagctcga agagccgcgc acggtcgcgg cccgccgcgg gctcgactac gaggcggtcg
     9121 ccccggct
//
LOCUS       DASTNL010000004         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1117422, whole genome shotgun sequence.
ACCESSION   DASTNL010000004 DASTNL010000000
VERSION     DASTNL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117422"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1423
                     /locus_tag="VFQ96_00155"
     CDS             65..1423
                     /locus_tag="VFQ96_00155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0256230.1"
                     /translation="MTTPTTPPDAGRRELTVALLGGGTVGSQVARLLHESADDLAARA
                     GAPLRLVGVGVRDTVRPREGIDQDLLTDDIEGLAASGADVVVEVLGGIEPARSLILAA
                     MAAGSSIVTANKALLAEDGATLHAAAEKHGVDLYYEASVAGAIPLLRPLRDSLAGDKV
                     RRVLGIVNGTTNFILSAMDENGADYADVLAEAQALGYAEADPTADVEGYDAAQKAAIL
                     AGLAFHTKIPLGSVYREGITGVTRAQVEAAIAAGYVIKLLAICERIERSSEGEAGVSV
                     RVYPALVPARHPLAAVHGAYNAVFVEAAAAGDLMFYGAGAGGVETASAVLGDLVSVAR
                     RHVAGGPGLIESTHAALPVLPVGRAITRYQVTLEVIDRPGVLARVARVISRAGVSVEA
                     MRQSASEARRPGGEEAGPATATLVIETHTAAESALASIVRALTGDEVVLSVTSVLRVE
                     GS"
     gene            1427..>2183
                     /gene="thrC"
                     /locus_tag="VFQ96_00160"
     CDS             1427..>2183
                     /gene="thrC"
                     /locus_tag="VFQ96_00160"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0256231.1"
                     /translation="MSERVSRQWRGVLREYGDRLDVTEATPIISLGEGGTPLVAAPAL
                     SERTGMDVWVKFEGANPTGSFKDRGMTMAVSKAVERGAKVVICASTGNTSASAAAYAA
                     YAGITAVVLVPEGKIAMGKLSQAIAHGARLLQVQGNFDDCLEIARELSANYPVHLVNS
                     INPDRIEGQKTAAFEIVDVLGDAPDFHILPVGNAGNYTAYTRGYREDLAAGRTTKLPR
                     MFGFQAAGSAPIVRGEPVANPETIASAIRIGNPA"
BASE COUNT          318 a          782 c          779 g          304 t
ORIGIN      
        1 gacctgccgg gtggcggcag gccgccgggt ggcacgctag gcgcacgacc tcggagggaa
       61 ccccatgacc accccgacca cgccgcccga cgcgggccgc cgcgagctga ccgtcgccct
      121 gctcggcggc ggcacggtgg gcagccaggt ggcacggctg ctccacgaga gcgccgacga
      181 cctcgccgcg cgggcgggtg ccccgctgcg cctcgtcggt gtcggcgtgc gcgacacggt
      241 gcggccgcgc gagggcatcg accaggacct gctgaccgac gacatcgagg gcctcgccgc
      301 cagcggcgcc gacgtcgtcg tcgaggtgct cggcggaatc gagccggcgc gctcgttgat
      361 cctggccgcg atggcggccg gctcgtcgat cgtcacggcc aacaaggccc tgctcgccga
      421 ggacggcgcg accctgcacg ccgccgcgga gaagcacggc gtcgacctgt actacgaggc
      481 ctcggtcgcg ggcgccatcc cgctgctgcg cccgctgcgc gacagcctcg cgggcgacaa
      541 ggtccgccgc gtcctgggca tcgtcaacgg caccaccaac ttcatcctct ccgcgatgga
      601 cgagaacggc gccgactacg ccgacgtcct ggccgaggca caggcgctcg gctacgccga
      661 ggccgacccg acggccgacg tcgagggcta cgacgcggcg cagaaggccg cgatcctcgc
      721 ggggctcgcc ttccacacga aaatcccctt ggggtccgtc taccgggagg ggatcaccgg
      781 ggtcacccgg gcgcaggtcg aggcggccat cgccgccggc tacgtcatca agcttctggc
      841 gatctgcgag cggatcgagc gctccagcga gggggaggcg ggcgtctcgg tgcgggtcta
      901 cccggctctt gtgccggcca ggcacccgct cgcggcggtg cacggcgcgt acaatgccgt
      961 gttcgtcgag gccgccgccg cgggcgacct gatgttctac ggcgcgggcg ccggcggcgt
     1021 ggagaccgct tcggcggtct tgggcgatct cgtctccgtc gcccggcggc acgtggccgg
     1081 cgggccgggg ctcatcgaat ccacccatgc cgcgctgccg gtgctgcccg tcggccgggc
     1141 gataacccgg tatcaggtga cgttggaggt catcgaccga cccggcgtct tggcccgcgt
     1201 cgcgcgggtg atcagccggg ccggcgtctc cgtcgaggcg atgcggcagt ccgcgagcga
     1261 ggcccgtcgg ccgggcggcg aggaggccgg gccggccacg gctacgctag ttatcgaaac
     1321 ccataccgca gccgaatccg cgctggcctc gatcgtgcgt gccttgaccg gcgacgaggt
     1381 cgtgctatcg gtgacatccg tgttgagagt cgaaggatcc tgattgatgt ctgaacgtgt
     1441 cagccgccag tggcggggcg tgctccggga gtacggggac cgcctcgacg tcaccgaagc
     1501 cacccccatc atcagcctcg gcgagggtgg caccccgctc gtggcggccc ccgctctctc
     1561 ggagcgcacg ggcatggacg tctgggtcaa gttcgagggc gccaacccga ccggatcgtt
     1621 caaagatcgc ggcatgacga tggcggtctc caaggcggtc gagcgcggcg cgaaggtcgt
     1681 catctgcgcc tctaccggca acacctccgc gtcggcggcc gcctatgccg cctatgccgg
     1741 gatcaccgcg gtcgtcctcg tgcccgaagg caagatcgcc atgggcaagc tgagccaggc
     1801 gatcgcacac ggcgcccgcc tgctgcaggt gcagggcaat ttcgacgact gcctcgagat
     1861 cgctcgcgag ctgtcggcaa actacccggt gcacctcgtc aactcgatca acccggatcg
     1921 catcgagggg cagaagacgg ccgcgttcga gatcgtcgac gtcttgggcg acgcccccga
     1981 ctttcacatc ctgcccgtcg gcaacgccgg caattacacg gcttacacgc gaggctatcg
     2041 ggaagacctc gccgccgggc gcacgacgaa gctgccgcgg atgttcgggt tccaggctgc
     2101 cggcagcgcg ccgatcgtgc gtggcgagcc ggtggcgaac cccgagacca tcgcgagcgc
     2161 gatccgcatc ggcaatccgg ctt
//
LOCUS       DASTNL010000005         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_933265, whole genome shotgun sequence.
ACCESSION   DASTNL010000005 DASTNL010000000
VERSION     DASTNL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933265"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..868)
                     /locus_tag="VFQ96_00165"
     CDS             complement(26..868)
                     /locus_tag="VFQ96_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256232.1"
                     /translation="MTSTRTLQLLFLSAAVAGLSACGGGGGSDAATTGAPAPAPAPGS
                     PPPPAPAPTSSGLPAATASLSQSASTAAGLAAKAADVYKKSAGAAAAVALGGAAPVAG
                     STSVACPGGGSVAYSGPATGMTATYNACVLGVHTFAGSATVSYVASGSTVQSYVIDYS
                     NGGVNVSAPNGFGATLTGRTSCDSSSGSRLCVADYQNVRWGNDFEYDYAGNLANGSTA
                     CDCTASQIVLVQAANLAATSGVANVTGASGSAVVTRTSATAFTVNLTAGGVTVSYPVT
                     VTSP"
     gene            1073..>2705
                     /gene="fusA"
                     /locus_tag="VFQ96_00170"
     CDS             1073..>2705
                     /gene="fusA"
                     /locus_tag="VFQ96_00170"
                     /inference="COORDINATES: protein motif:HMM:NF009891.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0256233.1"
                     /translation="MPSTVNPAVAAIRTLALVGQAAAGKTSLAEALLVRAGAIGAPGS
                     LERGSTVSDFDPLERKAQHSLNSSLMHLVHAETRVHLLDTPGYPDFLGQSLTALEAVE
                     TAAVVINAATGIEMMSERMMQWAAQRGLDRIVVVNKIDAQGVDLAGLVAQIQATFGRE
                     CLPLNLPAAGGSEVVDCFFNPQAPSGHEADFSSVEQAHRALVEQVVEVDPDFVERYLN
                     DGDVDPTELHAPLEQALREGHLIPIVFTSARNGAGIAELLDVIVKLLPNPTEGNPPQF
                     LKGEGAEARPLTAEPDPARHVLAHVFKVTQDPYVGKMGIFRIHQGTVTKDSQLYIGDG
                     RKPFKVGHLFMLQGREHVEVPRAVPGDICAVAKVDEMHFDAVLHDAAEDDHIHLKPLG
                     FPEPVHGLAIEPKRRGDEQRLWDILQKLVDEDPCLKVEHVVATNETVVYGLGELHLRT
                     LLERLTEVHHCEVNTRPPRIAYRETIAANAEGHHRHKKQTGGAGQFGEVFLKVEPLPR
                     GAGFEFVDQVKGGTIPSNFMPAVEKGIRQAMESGVV"
BASE COUNT          417 a          953 c          994 g          341 t
ORIGIN      
        1 gtaggcccgt gccgacgccc gacggtcagg gcgacgtgac ggtgacaggg tagctgacgg
       61 tcaccccgcc ggcggtcagg ttgaccgtga aggcggtcgc cgaggtgcgg gtaacgaccg
      121 ccgagccgct ggccccggtg acgttggcga cgcccgaggt cgcagcgagg ttggcggcct
      181 gcaccagcac gatctgcgag gccgtgcagt cgcaggccgt gctgccgttg gcgaggttgc
      241 cggcgtagtc gtattcgaag tcgttgcccc agcgcacgtt ctggtagtcg gccacgcaca
      301 gccgcgagcc gctggacgag tcgcagctcg tgcgcccggt cagcgtcgcg ccgaagccgt
      361 tgggcgcgct cacgttgacg ccgccgttgc tgtagtcgat gacgtagctc tgcaccgtcg
      421 agccgctggc gacgtaggag acggtcgcgc tgccggcgaa cgtgtgcacg cccagcacgc
      481 aggcgttgta ggtggcggtc atgccggtcg ccgggccgct gtaggcgacg ctgccgccgc
      541 ccgggcaggc gacgctggtc gaaccggcga cgggggcggc gccgccgagc gcgaccgccg
      601 ccgcggcccc ggcggacttc ttgtagacat cggccgcctt ggccgcgagg ccggccgcgg
      661 tgctggccga ctgcgacagg ctcgccgtgg ccgcgggcag gcccgaggag gtgggggccg
      721 gcgcgggcgg gggcgggctg ccgggcgcgg gcgccggcgc cggtgcaccg gtcgtcgcgg
      781 cgtcgctgcc gccgcccccg ccgcaggccg acaggccggc gacggcggca ctcagaaaca
      841 ggagctgcaa ggtacgcgtg gaagtcatgg tggtctcgct cggtccagaa agcgtggcag
      901 gaaacggctt cgggagccgc ggcctgcccc gggcaagccg catgcccgcg aggcgaagag
      961 agggggaagc accgggcgcg gacgcgtcgg cgggggcgag ccacctcggc ttgtgcgccc
     1021 gtcgcgcatc ccgtaccctc gacgcgagag ccaactccga ggagacccgt ccatgccttc
     1081 gaccgtgaac cccgccgtcg cggcgatccg cacactggcc ctggtcggcc aggccgccgc
     1141 cggcaagacc agcctcgccg aggcgctgct ggtcagggcg ggagcgatcg gcgcgcccgg
     1201 cagcctcgag cgcggcagca cggtgagcga cttcgacccg ctggagcgca aggcccagca
     1261 ctcactcaac tcgtcgctga tgcacctcgt gcacgccgag acgcgggtgc acctgctcga
     1321 cacgcccggc tatcccgact tcctgggcca gtcgctgacg gcgctggagg cggtcgagac
     1381 cgcggcggtg gtgatcaacg cagccaccgg catcgagatg atgagcgagc gcatgatgca
     1441 gtgggccgcg cagcgcgggc tcgaccgcat cgtcgtcgtc aacaagatcg acgcccaggg
     1501 cgtcgacctg gccggcctgg tggcccagat ccaggcgacg ttcgggcgcg agtgcctgcc
     1561 gctcaacctg ccggccgccg gcggcagcga ggtggtcgac tgcttcttca acccgcaggc
     1621 gccgagcggc cacgaagccg acttctcgtc ggtcgagcag gcgcaccgtg cgctggtcga
     1681 gcaggtggtc gaggtcgatc ccgacttcgt cgagcgctac ctcaacgacg gcgacgtcga
     1741 ccccaccgag ctgcacgcgc cgctcgagca ggcgctgcgc gaagggcacc tgatcccgat
     1801 cgtgttcacc tcggcgcgca acggcgccgg catcgccgag ctgctcgacg tgatcgtcaa
     1861 gctgctgccc aacccgaccg agggcaaccc gccgcagttc ctgaagggcg aaggcgccga
     1921 ggcccggccg ctgacggccg agcccgaccc ggccaggcac gtgctggcgc acgtgttcaa
     1981 ggtcacccag gacccgtacg tcggcaagat gggcatcttc cgcatccacc agggcacggt
     2041 caccaaggac agccagcttt acatcggcga cggccgcaag ccgttcaagg tcggccacct
     2101 gttcatgctg cagggccgcg agcacgtcga ggtgccgcgc gccgtgccgg gcgacatctg
     2161 cgccgtcgcc aaggtcgacg agatgcactt cgacgcggtg ctgcacgacg ccgccgagga
     2221 cgaccacatc cacctgaagc cgctcgggtt ccccgagccg gtgcacggcc tggcgatcga
     2281 gcccaaacgg cgcggcgacg agcagcgcct gtgggacatc ctgcagaagc tggtcgacga
     2341 ggacccgtgc ctgaaggtcg agcacgtcgt cgccaccaac gagaccgtcg tctacgggct
     2401 gggcgaactg cacctgcgca cgctgctcga gcggctgacc gaggtgcacc actgcgaggt
     2461 caacacgcgc ccgccgcgca tcgcctaccg cgagacgatc gcggccaacg ccgaaggcca
     2521 ccaccggcac aagaagcaga ccggcggcgc cgggcagttc ggcgaggtct tcctcaaggt
     2581 cgagccgttg ccgcgcgggg cgggcttcga gttcgtcgac caggtcaagg gcggcacgat
     2641 cccgagcaac ttcatgccgg cggtcgaaaa gggcatccgc caggcgatgg agtcgggcgt
     2701 ggtcg
//
LOCUS       DASTNL010000006         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_191967, whole genome shotgun sequence.
ACCESSION   DASTNL010000006 DASTNL010000000
VERSION     DASTNL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191967"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /gene="hisE"
                     /locus_tag="VFQ96_00175"
     CDS             <1..114
                     /gene="hisE"
                     /locus_tag="VFQ96_00175"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027788.1"
                     /note="catalyzes the formation of 1-(5-phosphoribosyl)-AMP
                     from 1-(5-phosphoribolsyl)-ATP in histidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphatase"
                     /protein_id="HEU0256234.1"
                     /translation="EHEGRERTAEEISQLLYHVQVMMIDRGLSLEDVWRYL"
     gene            158..997
                     /gene="hisG"
                     /locus_tag="VFQ96_00180"
     CDS             158..997
                     /gene="hisG"
                     /locus_tag="VFQ96_00180"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU0256235.1"
                     /translation="MIRIAVPNKGILAQTAAQMLAEAGYHGRNDTKDLIVADPANDAE
                     FFYLRPRDIATYVGGGALDVGITGRDLLLDSGSSAIELDALGFGRSQFRFAGPADAFQ
                     EPADLNGTRVATSYPGLVGRYLTDHGVTVRLVTLDGAVESAVRLGVADAVADVVQTGA
                     TLRKQGLRIFGPVILDSEAVLIGNGHRPEGLATLKRRLDGVIVARRFVLIEYDLPADL
                     LEQAVALTPGSESPTVSPLRDAGWVAVRAMVSREKTNDVMDDLYDLGARAILVTPIHA
                     ARM"
     gene            1000..1764
                     /gene="hisF"
                     /locus_tag="VFQ96_00185"
     CDS             1000..1764
                     /gene="hisF"
                     /locus_tag="VFQ96_00185"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEU0256236.1"
                     /translation="MTLATRVIPCLDVSGGRVVKGVNFAHLRDAGDPVELARRYGSEG
                     ADEITFLDVTATVDGRDTMYDVVRATAEQVFIPLTVGGGVRSVDDVARLLASGADKVG
                     VNSAAIARPALIEEIAARYGSQVLVLSLDITRDTETASGFVVTTHGGRTRTGIDALEW
                     ARRAIDAGAGELLVNSIDADGTRDGFDLELVAAVRRLSTVPVIASGGAGRAADFPPAV
                     AAGADAVLAASIFHSGAVRIGEVKDALRQAGVEVRS"
     gene            1761..2150
                     /gene="hisI"
                     /locus_tag="VFQ96_00190"
     CDS             1761..2150
                     /gene="hisI"
                     /locus_tag="VFQ96_00190"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEU0256237.1"
                     /translation="MTDDRVDTVAGAAERIRFGADGLVPAIIQQWDSGEVLMMGWMDR
                     EAFRRTMTTGRVTFWSRSRQEYWRKGDTSGHRQYVRGAALDCDGDTLLVRVEQIGAAC
                     HTGAHSCFDVDPLQPVVLPDTKDTTTP"
     gene            2147..>2760
                     /locus_tag="VFQ96_00195"
     CDS             2147..>2760
                     /locus_tag="VFQ96_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU0256238.1"
                     /translation="MTDAQTTKPAAGAPPAFAGEGTMTRAAFDGLFAGHRVIPVTREL
                     FADAETPTGVYRKLAHGRPGTFLLESAEQGGIWSRFSFVGVASFGVLTQDGDRAVWLD
                     YGLGAEAALGADAPRAPLDALARMHERWHTPRAAGLPPLTGGLVGHIGWEAVRQLERL
                     PGGPPADYDVPGQAMCFVSDLVVIDHRRGTLQMVATVLSGAQAPS"
BASE COUNT          409 a          977 c          960 g          414 t
ORIGIN      
        1 gagcacgagg gccgcgagcg cacggccgaa gagatctcgc aactgctcta ccacgtccaa
       61 gtcatgatga tcgaccgggg cctttcgctg gaagacgtgt ggcgctatct ctgagacctg
      121 ccacacccga cccgacccga aagagacctg agacccgatg atcagaatcg ccgtgcccaa
      181 caagggcata ctcgcgcaga ccgcggcaca gatgctcgcc gaggccgggt accacggccg
      241 gaacgatacg aaagacctga tcgtggccga cccggccaac gacgcagagt ttttctattt
      301 gaggccgcgt gacatcgcca cctatgtcgg cggcggcgca ctcgacgtcg gcatcacggg
      361 ccgtgatctg ctgctcgact ccggctcgtc ggcgatcgag ctggacgcgc tcggtttcgg
      421 tcgttcgcag tttcgtttcg cggggccggc cgacgccttc caggagccgg cagacctgaa
      481 cggcacgcgg gtcgcgacga gctacccggg cctcgtcggc cggtacctca ccgaccacgg
      541 tgtcacggtg cgcctcgtga ctctcgacgg ggcggtcgag tccgccgtgc ggctcggcgt
      601 ggccgacgcg gtggccgacg tcgtgcagac cggggcgacg ctgcgcaaac agggccttcg
      661 catcttcggc ccggtcatcc tcgactccga ggcggtgctg atcggcaacg ggcaccgccc
      721 cgaagggctc gccacgttga agcgccgcct cgacggtgtc atcgtggccc ggcgtttcgt
      781 cctcatcgaa tacgacctgc cggcagacct cttggagcag gccgtcgcgc tgacccccgg
      841 cagtgagtcg ccgacggtct caccgttgcg cgacgccggg tgggtcgcgg tgcgcgccat
      901 ggtgtcgcgg gagaagacca acgacgtcat ggacgacctg tacgatctcg gtgccagggc
      961 gattctcgtg acgccgatcc acgctgcaag gatgtgaccg tgacgctggc gacacgtgtc
     1021 atcccctgcc tcgacgtgtc gggcggccgg gtggtcaaag gcgtcaactt cgcccacttg
     1081 cgggatgccg gcgaccccgt ggagctggcc cgccgctacg gctccgaggg agccgacgag
     1141 atcaccttcc tcgacgtgac ggcgacggtg gacgggcgcg acacgatgta cgacgtcgtc
     1201 cgggccaccg cggaacaggt cttcatcccg ctcacggtcg gcggcggggt gcgcagcgtc
     1261 gacgacgtgg cgcgcctgct cgcctcgggc gccgacaagg tgggcgtcaa cagcgccgcg
     1321 atagcccggc ccgcgctgat cgaagagatc gccgcccgct acggctcaca ggtcttggtg
     1381 ctctctctcg atatcacgcg cgataccgag actgcctccg ggttcgtcgt cacgacgcac
     1441 ggcggccgca cccgtaccgg tattgacgcg ctggagtggg cgcgccgggc catcgacgcc
     1501 ggcgcggggg agcttctcgt caactcgatc gacgccgacg gcacccgcga cggtttcgac
     1561 ctggagcttg tggcagccgt gcgccggctc agcaccgtgc ctgtcatagc gtcgggcggt
     1621 gcgggccggg cggccgattt tccgccggcc gtcgccgccg gcgccgacgc ggtgctggcg
     1681 gccagcatct tccattccgg tgcggtgcgc atcggcgagg tcaaggacgc gctacgtcaa
     1741 gcaggggtgg aggtccgctc gtgaccgacg acagggtcga cacggtcgcc ggggcggccg
     1801 aacgcatccg cttcggcgcc gacgggctgg tcccggccat cattcaacag tgggacagcg
     1861 gcgaagtgct catgatgggc tggatggatc gcgaggcgtt ccgccgcacc atgacgaccg
     1921 ggcgggtcac cttctggtcc cgctcccgcc aggagtactg gcgtaagggc gacacgtcag
     1981 ggcaccggca gtacgtacgc ggcgccgcgc tcgactgcga cggcgacacg ctcctcgtca
     2041 gggtcgagca gatcggggcg gcctgccaca ccggcgcgca cagctgcttc gacgtcgacc
     2101 cactgcaacc cgtggtgctg cccgacacga aggacacgac gacgccatga ccgacgccca
     2161 gacgacgaag cccgccgccg gcgcccctcc ggcttttgcc ggcgagggga cgatgaccag
     2221 ggcagcgttc gacggcctgt tcgccggcca tcgcgtgatc cccgtcaccc gcgagctttt
     2281 cgccgacgcc gagacgccga ccggggtcta ccgcaagctg gcgcacggcc ggcccggcac
     2341 gttcctcttg gagtcggccg agcagggcgg catctggtcc aggttctcgt tcgtcggcgt
     2401 cgcctcgttc ggcgtgctca cccaagacgg cgaccgtgcc gtgtggctcg actacggcct
     2461 cggcgccgag gccgccctgg gcgccgacgc gccccgggcg ccgctcgacg ccctcgcccg
     2521 catgcacgag agatggcaca cgccgcgggc cgccggcctg cctccgctga ccggcgggct
     2581 cgtcggccac atcggctggg aggcggtgcg acagctcgag aggctgcccg gcgggccgcc
     2641 ggcggattac gacgtgcccg ggcaggcgat gtgtttcgtc tccgatctcg tcgtgatcga
     2701 ccaccgacgc ggaaccctgc agatggtcgc gacggtgctc tccggcgccc aggcaccctc
//
LOCUS       DASTNL010000007         5339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_379173, whole genome shotgun sequence.
ACCESSION   DASTNL010000007 DASTNL010000000
VERSION     DASTNL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379173"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFQ96_00200"
     CDS             complement(<1..288)
                     /locus_tag="VFQ96_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256239.1"
                     /translation="MSGSFHSGELEVQRRAGRSSGAVIRSSIPEIAAEFLVSRPARVI
                     AGRDGEGRMWATMLTGEPGFMQTPDPRVMVVHAEPRPDDPLAPLLAQGGEVG"
     gene            complement(339..815)
                     /locus_tag="VFQ96_00205"
     CDS             complement(339..815)
                     /locus_tag="VFQ96_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0256240.1"
                     /translation="MMSTSTATPTLTSLKTGHIGLNVTDLERSQAFYERLLGLSVAAE
                     GSDDDRRWVFLGRDGALVVTLFQQSKGTFSTSTPGLHHLSFQVEDMEQVHAAEAIAHE
                     LGAPFFHESVVAHSEGSSSGGIFFADPDGIRLEVYAPHGAEDAPAPSGEAPTCGFF"
     gene            958..1536
                     /locus_tag="VFQ96_00210"
     CDS             958..1536
                     /locus_tag="VFQ96_00210"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU0256241.1"
                     /translation="MERPLVGEPLAVDLVDTRWHGDAGDEDLLATPAGLTQWLRERGL
                     DSPLRRPAEGAGDPSGEPGGVLERMRAALLHTRAVLRRAFEGEARAQDGVNEVLAHAV
                     IVRSLEAGRVRDIPMFPDEAWRPAWLAADDYVRLLQAAPDGIRKCGNPSCVLYFFDPG
                     GRRRWCSMARCGNRLKAQRHYARTRLAARPEM"
     gene            1691..2413
                     /locus_tag="VFQ96_00215"
     CDS             1691..2413
                     /locus_tag="VFQ96_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0256242.1"
                     /translation="MSLPRVKLLERQEVARNTIVLRFEKPEGFEFRAGQYADYTLLEP
                     TETDAEGDTREFTFSSAPFEDHLACTIRLRDTAFKRVVQKLPVGAEVELDAPYGSFVL
                     HRKAARPAVFMTGGVGVTPARSILLQADHDHTGHKITLFYSNKTPADAAYLDEFTALS
                     AANPDITFVPTMTAPGAAQSGWQGHLGRIDGALLAEHVPDLAAPIYYLCGPASMVRSM
                     RSVLAEAGVDDDDIRVEEFLGY"
     gene            2464..2727
                     /locus_tag="VFQ96_00220"
     CDS             2464..2727
                     /locus_tag="VFQ96_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEU0256243.1"
                     /translation="MASGESRRVTMYGADWCGDCRRTKAQLDGLGVPYDFVDLVVRPE
                     AAETARAIAGRTNIPVVVYPDGEHAVEPTDAALAAKVRELGLA"
     gene            2803..4281
                     /locus_tag="VFQ96_00225"
     CDS             2803..4281
                     /locus_tag="VFQ96_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256244.1"
                     /translation="MPHTVPIADTSVPERLDRLPYTRRHSRILIGSGIGWTLDAMDVG
                     LISFIIAALIRDWGIDKGTGGLIASVGFVGMAIGASVGGLLADRLGRRSVFALTLLIY
                     GVATGASALAGGVAVLLVLRFFVGLGLGAELPVASTYVGEFAPARIRGRLVVILEAFW
                     ALGWTASAVIGYLVIPASSAGWRWAFALGAVPAAYAIVVRLRLPESARWLARRGRHAE
                     ADRVTATLEAAAYEPRVSDGGVTPGGTLRRGWASRLLSPARETQNEQVASHAPPSEAP
                     AAAPAVARAATPLGRNAGARLAALFSHEFRRRTVALWAVWVCVNFAYYGAFIWIPSIL
                     VDDGYDLVRSFGFTLIITLAQLPGYAAAAWLIEVWGRPVTLTVFLAGSAGSALGFGTA
                     HGAFALVAFGMALSFFNLGAWGVLYAVTPEIYPTSLRATAAGWAAAIGRVASIAAPLT
                     VPVLLVAGGAALTFTVFALFFLVAAAASWGLASPRGARLDER"
     gene            complement(4297..4752)
                     /locus_tag="VFQ96_00230"
     CDS             complement(4297..4752)
                     /locus_tag="VFQ96_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEU0256245.1"
                     /translation="MPHYLEDLHAGQVFVTPGRTISEADVMSFAAWTGDNNQVHTDAV
                     FAAKTRYGRRVVHGMLGASLCLGLIARTGVFEGSAVALLGVDRWRFTAPVFIGDTLTA
                     TVEILSTRPTSNGRNGIVERQVTLRNQDGVTVQEGRMDLMVLTRAAALA"
     gene            complement(4801..>5339)
                     /locus_tag="VFQ96_00235"
     CDS             complement(4801..>5339)
                     /locus_tag="VFQ96_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HEU0256246.1"
                     /translation="LAAWNTFVVDEHARVPTPGIAVMLERLAAEHPGGPVVYLSTGAW
                     NVAPALTRFLSRNLYPAGPLLLTDWGPTHDRLFRSGIEHKRQSLERLHDEFPGMRWLL
                     LGDDGQHDEEVYDEFAVRHPESVAAVAIRQLSPSEAVLAGRRKKASVRTLRSRVPWIA
                     APDGAGLVQQLTELGILG"
BASE COUNT          737 a         1830 c         1894 g          878 t
ORIGIN      
        1 gccgacctcg ccgccctggg caaggagcgg cgcgaggggg tcgtcgggcc gcggctcggc
       61 gtgcaccacc atgacgcgcg ggtcgggggt ctgcatgaat cccggctcac ccgtcagcat
      121 ggtggcccac atgcgcccct cgccgtcgcg gccggcgatg acacgcgccg gccgcgagac
      181 gaggaactct gccgcgatct ctggaatgga actgcgtatg accgcaccgg aactccggcc
      241 ggccctgcgc tgtacctcaa gctcacccga gtggaatgat ccgctcatcg cgttcttctt
      301 ccgatgtgcg tgttagagac gatctgcggc atggtggatc agaagaaccc gcaggtcggt
      361 gcctcaccgc tgggtgccgg ggcgtcttct gccccgtggg gtgcgtaaac ctcgaggcgg
      421 atgccgtcgg ggtcggcgaa aaagatgccg cccgaactcg acccctccga atgtgcgacg
      481 acgctctcgt ggaagaacgg cgcccccagt tcgtgggcga tcgcctcggc cgcgtgcacc
      541 tgctccatgt cttcgacctg gaacgagagg tggtgcaggc ccggcgtcga cgtgctgaac
      601 gtgcccttgc tctgctggaa caaagtcacg accagcgccc cgtcgcggcc caggaagacc
      661 cagcgccgat catcgtcgct gccttccgcc gcgacgctga ggcccaggag tcgctcgtag
      721 aaggcctggg atcgttccag gtcggtgacg ttcaacccga tatgaccggt cttcagcgag
      781 gtcagcgtcg gtgttgccgt gcttgtgctc atcattgcct ttccgggcgt ctgggatgcc
      841 ctcatagccg tgcccctaac catctcagaa gcacgataag gataggctgg ccggtttata
      901 ccggacccat cgcttagata ctaacccctt agtgccgcgg agtggttaga tagaggcatg
      961 gaacgacccc tggtcggcga gcctcttgcc gtggacctcg tcgatacccg ctggcacggc
     1021 gacgcgggcg acgaggatct cttggcgacc ccggcggggc tcacccagtg gctgcgcgag
     1081 agggggctgg actcgcccct ccgccgcccc gccgaggggg ccggcgaccc gtcgggggag
     1141 ccgggcggcg tgctggagcg gatgcgtgcc gccttgttgc acacccgggc ggtgttgcgc
     1201 cgggcgttcg agggtgaggc tcgggcgcaa gacggtgtga acgaggttct cgcccacgcg
     1261 gtgatcgtgc gcagcctcga ggccggccgc gtccgcgaca tcccgatgtt ccccgacgag
     1321 gcgtggcggc cggcctggct ggcggccgac gactacgtgc ggctcctgca ggcggccccg
     1381 gacggcatcc ggaagtgcgg caacccctcg tgtgtgctct atttctttga cccgggcggc
     1441 cgccggcgtt ggtgttcgat ggcgcgctgc ggcaaccggc tgaaggcgca acgccactac
     1501 gccaggacac ggctcgccgc caggcccgag atgtagcggg cggagcctgc ccggatggcc
     1561 gggcggcgtc gcgcgggggc tcggtgccgc cggatcgagg cccgcggccc gggaggagga
     1621 ttccgccggc cggaaggtgc cgagatgcgc cgtgtcgaag acgaggaagg atgggtttct
     1681 ggtgcccgat atgtcgttgc ccagggtgaa gctgttggag aggcaggagg tcgcgcggaa
     1741 cacgatcgtg cttcggttcg aaaaaccgga ggggttcgag ttccgagcag ggcaatacgc
     1801 ggactacacc ctgcttgagc ccaccgagac cgacgccgag ggcgacacac gagagttcac
     1861 gttttcgagc gcgccgttcg aagatcacct ggcctgcacc atcaggctgc gtgacacggc
     1921 gttcaagcgg gtcgtgcaga agctgccggt cggcgccgag gtggagctgg acgcgcctta
     1981 cggctcgttc gtactgcacc gcaaggcggc ccggcccgcg gtgttcatga ccggcggggt
     2041 gggagtcacc cccgcccgca gcatcctgct gcaggccgac cacgatcaca ccgggcacaa
     2101 gatcaccctt ttctactcga acaagacgcc ggccgacgcg gcctacctcg acgagttcac
     2161 agctttgtcg gccgcgaatc ccgacatcac cttcgtgccg acgatgaccg cacccggggc
     2221 ggcccaatcc ggttggcagg gacacctcgg tcgtatcgac ggggcgctct tggccgagca
     2281 cgtgcccgac ctggcggcgc cgatctacta cctgtgcggc cccgcgtcga tggtgcgctc
     2341 gatgcgctcc gtgctcgcgg aggccggcgt cgacgacgat gacatccgcg tcgaagagtt
     2401 cctcggctat tgaacgcgcc ggggccgcgc gccgcgggcg gcacggcgcg agataatcga
     2461 gtcatggcat ccggtgagtc gcggcgcgtc accatgtatg gcgccgactg gtgcggcgat
     2521 tgccggcgca ccaaggcaca actcgacggg ctgggcgtgc cgtacgactt cgtcgacctc
     2581 gtcgttcgcc ccgaggctgc ggagacggcg cgggcgatcg ccgggcgcac gaacatcccg
     2641 gtcgtcgtgt atccggacgg agagcacgcc gtcgaaccga cggatgccgc actggcggcg
     2701 aaggtgcggg agctcgggct ggcctgaggc gctttccgcg ccccgcaggt agcggggcgt
     2761 cacacttcga catgatttct gtatcccgcc tatcataggc ggatgcctca tacggttccc
     2821 atagcagaca cctcggtacc tgaacggctc gaccggctgc cgtacacgcg caggcactcc
     2881 cgcatcctga tcggctccgg catcggctgg accctcgacg cgatggacgt gggcctgatc
     2941 tcgttcatca tcgcggcgct catccgcgat tggggaatcg acaagggcac cggcgggctg
     3001 atcgcttcgg tcggtttcgt cggcatggcg atcggggcga gtgtcggcgg gctcttggcc
     3061 gaccggctcg ggcggcgctc cgtgttcgcc ctgacccttc tcatctacgg ggtcgcgacg
     3121 ggggcgagcg ccctcgcggg cggcgtcgcc gtgctcctgg tcttgcgatt cttcgtcggg
     3181 ctgggcctgg gcgccgagct gcctgtcgcc tccacctacg tgggggagtt cgcaccggca
     3241 cggatcagag ggcgactcgt cgtgatcctg gaggcgttct gggcgctcgg ctggacggcg
     3301 tcggccgtca tcggctatct cgtcataccg gcgtcgtcgg ccggctggcg gtgggcgttc
     3361 gcgttgggcg cggtgcccgc cgcctacgcg atcgtcgtac ggctgcggct gcccgagtcg
     3421 gcccgctggc ttgcacggcg tggccggcac gccgaggcgg atcgcgtcac cgccacgctc
     3481 gaggccgcgg cttacgagcc ccgggtctct gacggcggcg tcacgccggg cggcacgctg
     3541 cggcgaggat gggcctcacg cctcctttcg cccgcccgcg agacgcagaa cgaacaggtc
     3601 gcctcgcacg ccccgccctc ggaggccccg gcggctgccc cggccgtggc gcgggcggcg
     3661 actccgctcg gccggaacgc cggcgcgcgc ctcgccgccc tcttctccca cgagttccgc
     3721 cggcgcaccg tggccctttg ggcggtgtgg gtgtgcgtca atttcgccta ctacggggcc
     3781 ttcatctgga tccccagcat cctcgtcgac gacggctacg acctcgtgcg ctcgttcggc
     3841 ttcacgctta tcatcacgct ggcccagctg ccgggttacg ccgcggccgc ctggctcatc
     3901 gaggtgtggg ggcggcccgt cacgctcacc gtctttctgg ccggctccgc cggctcggcg
     3961 ctcggcttcg gcaccgccca cggcgccttc gccctcgtgg ccttcggcat ggcgttgtcg
     4021 ttcttcaact taggcgcgtg gggggtgctc tacgccgtca cgcccgaaat ctacccgacg
     4081 tcgttgcgcg ctaccgccgc ggggtgggcg gcagcaatcg gccgcgtcgc ctcgatcgcg
     4141 gcgccgctga cggtgcccgt gttgctcgtg gccggcggcg ccgcgctcac gttcacggtg
     4201 ttcgcactct tcttcctcgt cgccgcggcc gcgagctggg gccttgcgag cccgcgcggc
     4261 gcgaggctcg acgaacgttg atgtcggggt gcgccgtcag gcgagcgcgg cggcgcgggt
     4321 cagcaccatg aggtccatcc ggccctcttg caccgtcacg ccgtcttgat ttctgagcgt
     4381 gacctggcgt tctacgatgc cgttacggcc gttgctggta ggtctcgtcg aaaggatctc
     4441 caccgtggcg gtgagcgtgt cgccgatgaa gacgggcgcc gtgaaacgcc accggtcgac
     4501 gcccagcaag gcgacggcgc tgccctcgaa gactcccgtg cgggcgatga gccccaggca
     4561 gagagaggcg cccagcatgc cgtgcacgac ccgccggccg tagcgggtct ttgcggcgaa
     4621 gaccgcgtcg gtgtgcacct ggttgttgtc acccgtccac gcggcgaacg acatgacgtc
     4681 ggcctcgctg atcgtgcggc ccggcgtcac gaagacctgc cccgcgtgca ggtcttcaag
     4741 gtagtgcggc accgcgccag tatgccacac gcgcaggtgc cggggccggg cgatgcgccc
     4801 ctagccgaga atgccgagct cggtcagctg ctgcacgagc cccgccccgt cgggcgcggc
     4861 gatccacgga acccgggagc gtaacgtccg aaccgaggcc ttcttgcgcc gccccgcgag
     4921 caccgcctcg ctcggggaga gctggcggat cgcgacggcg gcgacgctct ccgggtgccg
     4981 cacggcgaac tcgtcgtaca cctcctcgtc gtgctggccg tcgtcgccga gcagcagcca
     5041 gcgcatgccg ggaaactcgt cgtgcaagcg ttctaggctc tgccgtttgt gttcgatgcc
     5101 gctgcggaag agccggtcgt gggtcggccc ccagtcggtg aggagcaggg ggcccgccgg
     5161 gtagaggttg cgggagagga accgggtgag cgccggagcg acgttccacg cgccggtcga
     5221 caggtagacg acgggcccgc cggggtgctc cgccgccaag cgttccagca tgacggcgat
     5281 acccggcgtc ggcacgcgcg cgtgctcgtc aaccacgaac gtgttccagg ctgcgagga
//
LOCUS       DASTNL010000008         8992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_380344, whole genome shotgun sequence.
ACCESSION   DASTNL010000008 DASTNL010000000
VERSION     DASTNL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380344"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFQ96_00240"
     CDS             complement(<1..183)
                     /locus_tag="VFQ96_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256247.1"
                     /translation="MISTGGVRHRLPPGPGDLVLRGGGQTSVATDTLLEAGRALDAIA
                     TGLEAGVARARRRMAGI"
     gene            complement(180..632)
                     /locus_tag="VFQ96_00245"
     CDS             complement(180..632)
                     /locus_tag="VFQ96_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256248.1"
                     /translation="MVTGTSRLDETAFEPSLAGLSLPGVGWERPAGPTAGLAWADPAT
                     LRMLLRRATRRRDGLAVLARDLDEVAEGLAEGARRVPSRVPDWHSLSAGAYAEAATRL
                     CDRAGRVSVALSQTGGLVRAEIRALDAECLRIRAELEARVARAWAGAP"
     gene            863..2260
                     /gene="purB"
                     /locus_tag="VFQ96_00250"
     CDS             863..2260
                     /gene="purB"
                     /locus_tag="VFQ96_00250"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0256249.1"
                     /translation="MNALPAQPLSPLDGRYRPVVEGLGVELSEAGLNRARLRVETEWL
                     IYLTGHRMFGSTPLRADDQAALSRLATDFGPDDIAELAALEAETRHDVKAVEYFVRRR
                     LAERGLESIAELTHFGATSEDINNIAYALTVREAVTTVWLPAFDTVVQALTERALAFR
                     GDAMLAHTHGQPATPTTMGKELAVFVYRLRRIRGQIVASDYLAKFSGATGTFAADVAA
                     DPDVDWRQVSREFVGSFGLTWNPLTTQIEPHDWQAELYARLTHANRVLHNLATDLWTY
                     ISLGYFRQVPQAGATGSSTMPHKINPIRFENAEANLELSCAVLDSLSATLVTSRMQRD
                     LTDSTTQRNIGVGLGHSLLALDNLRRGLDALDLDRERLAYDLDANWEVLGEAIQTVIR
                     AEVVAGRSHIADPYALLKGLTRGKRIGRAELAAFLDGLEIGDAARERLRALTPARYIG
                     LAESLVDELTTAPAL"
     gene            complement(2434..2748)
                     /locus_tag="VFQ96_00255"
     CDS             complement(2434..2748)
                     /locus_tag="VFQ96_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256250.1"
                     /translation="MSNQGDGAEKKIPPTPMPLPGMAYEPTRRDRLRPVELVGFSAIL
                     AVFTAIIVLAATREWWLTGISLAVSFIIVLVIVALLSMSFQPKADEVEELEEQDREDK
                     GH"
     gene            complement(2732..3352)
                     /locus_tag="VFQ96_00260"
     CDS             complement(2732..3352)
                     /locus_tag="VFQ96_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU0256251.1"
                     /translation="MPIARASVAFVAAVIITFLANHSTQVGLLVFGGYGVAAGVLVGV
                     LALRTSPRGIVRALFVAIGALGLVFGAAALALHGGGLEALIALVSAWAVLTGALELYA
                     GVRSRKRLAAARDWRAVGVLSLALAVAYLIVPPGLHKHFAGQDGVHGTLTAAIVLVGI
                     FGAYAAVVCVYWVIGGLSLRWAAVDEARSGGQQDSRDRKAPDEQPG"
     gene            complement(3523..4848)
                     /gene="dnaB"
                     /locus_tag="VFQ96_00265"
     CDS             complement(3523..4848)
                     /gene="dnaB"
                     /locus_tag="VFQ96_00265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0256252.1"
                     /translation="MGVEERTPPHDLLSEQSTLGAMMLSKDAVADVVETVRGADFYVP
                     KHEIIFGAILDLYSHGEPTDVITVTDELTKQGNLTRAGGAEYLHTLTGIVPTAANAGY
                     YATIVAERALLRRLVDAGTRIAQMGYAGQGDAVDLVNDAQAEIYAVTGSNDSEDYVPL
                     TQAVTAAIDEIESARHKDGAMTGVPTGFADLDELTNGLHPGQMIIVAARPALGKSTLA
                     LDFARSAAIKHDLPTILFSLEMGRSEIAMRLLSAESSVPLQSMRKGTVESRDWTTIAS
                     TRGRINDAPLYIDDSPNMTLVEIRAKCRRLKQRVGLKMVVIDYLQLMTSGKRVESRQQ
                     EVSEFSRALKLLAKELQVPVVALSQLNRGPEQRQDKLPALSDLRESGSIEQDADVVIL
                     LHRESAYDRDNPRAGEADLIVAKHRNGPTRTITVAFQGHYSRFADMVRD"
     gene            complement(5295..5747)
                     /gene="rplI"
                     /locus_tag="VFQ96_00270"
     CDS             complement(5295..5747)
                     /gene="rplI"
                     /locus_tag="VFQ96_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0256253.1"
                     /translation="MSKLILTNEVSGLGQAGDVVDVKTGYARNYLIPQGFAVAWTRGG
                     AKQVDQIKAARLAREHATVEEALDLKARLEATSLKLSVKAGREGRLFGSVKAADIASA
                     AEAAGLAGVDRRKIEIPVPIKSVGTYQALLRLRDDITATITIEVAASK"
     gene            complement(5760..6026)
                     /gene="rpsR"
                     /locus_tag="VFQ96_00275"
     CDS             complement(5760..6026)
                     /gene="rpsR"
                     /locus_tag="VFQ96_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU0256254.1"
                     /translation="MAGKSSGDRRKNRKGKIKDGKPVAPARTVHVGVIDYKDVATLRK
                     FISERGKIRARRITGVSVQEQRLIARAVKNAREMALLPYAGSGR"
     gene            complement(6065..6556)
                     /locus_tag="VFQ96_00280"
     CDS             complement(6065..6556)
                     /locus_tag="VFQ96_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0256255.1"
                     /translation="MAGETVITVIGNLTADPELRYTQSGVAVASFTVASTPRTFDRAS
                     NEWKDGEALFLRCSAWRDVAEHVAGSLTKGARVIVQGRLRQRSYETREGEKRTVIELT
                     VDEVGPSLRYATATLTRTSSSGGRGPAASADNEPWAPSAPAAPSGGDVWNTPGTFNDE
                     TPF"
     gene            complement(6562..6933)
                     /gene="rpsF"
                     /locus_tag="VFQ96_00285"
     CDS             complement(6562..6933)
                     /gene="rpsF"
                     /locus_tag="VFQ96_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0256256.1"
                     /translation="MHQYELMVILDPGIDERTVAPSIDKFLGVIRGDGGSIEKVDVWG
                     RRRLAYPIQKKTEGIYAVVDFTANPAATQELERQLKLSEAVMRTKVLRAEEAIAQVAQ
                     AAKRAARKASRAAALRPAKAE"
     gene            complement(7079..8317)
                     /locus_tag="VFQ96_00290"
     CDS             complement(7079..8317)
                     /locus_tag="VFQ96_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0256257.1"
                     /translation="MTERSITAVIVGAGHSGLAMSARLTARGVDHVIFERGEIAQSWR
                     SERWDSLHLLTPNWQTGLPGYDYDGTDPDGYLSMPEVVSRISSYAQIIDAPVRAHTAV
                     TKVTPTDEGYRVETDDGVWQARHVIVASGTARRPNLPAAAAAIPAEVRQITPLSYRNP
                     DQLGAGGVLVVGASASGAQLADEIARSGRRVVVSSGELVRMPRVYRGRDVFWWMDRAG
                     VLDERYDEMDDIVRARHVPSPQLVGSKARRDLDLGTLQELGVEIVGKLGMVRDGTALF
                     SGGLANVARLADLKEERLLDRFDEWAKEHGDITEAPYRPDPVTVPARPRLSLDLVREG
                     IETVVWATGFAPDYSWLDVDTFDRKGRIVHDGGVGRAHGLYTLGTSLLRRRRSTYLSG
                     AAADTEDLARHLVSALALAH"
     gene            complement(8337..8906)
                     /locus_tag="VFQ96_00295"
     CDS             complement(8337..8906)
                     /locus_tag="VFQ96_00295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0256258.1"
                     /translation="MTTTQTPHAPVDNGVSSDALVAAREQLRDAPEAAEFVWRATSEW
                     QSGTHSTQTIEKYTGLGTERAHKSVFQLEGDHPDIFAGNDEAPTPVETVLAALAGCLT
                     AGIAAVAQRREIQLRSVKATLEGKENILGILGADPDVRNGFNEIDVHFDIDADATEEE
                     IAALVAQSQKRSAVFDALTNPTTVNVSVG"
BASE COUNT         1429 a         3079 c         3037 g         1447 t
ORIGIN      
        1 gattccggcc attcggcgac gcgcccgtgc cacgcctgcc tcgaggccgg tcgctatcgc
       61 gtcgagagcc cggcccgcct cgaggagcgt gtcggtggcg acggaggtct gcccgccgcc
      121 tcgcaggacg agatcaccgg gccccggagg caggcgatgc cggacccctc cggtcgagat
      181 cacggagccc cggcccacgc ccgcgccacc cgtgcctcaa gctcggcacg gatccgcagg
      241 cactccgcat cgagggcgcg gatctccgcc cgaacgagcc cgcctgtctg tgacagggcc
      301 acactcaccc ggccggcacg gtcacaaagc cgggtagccg cctcagcgta ggcgcctgcc
      361 gagagtgaat gccagtctgg gacacggctc ggcacccgcc tcgccccctc cgcaagaccc
      421 tcggccacct cgtccaggtc acgggcgagc accgccaggc cgtcacggcg gcgggtggca
      481 cgccgcaaca gcatacgaag cgtcgccgga tcggcccacg cgaggcctgc cgtcgggccc
      541 gcgggccgct cccacccgac gcccggcaac gacaagccgg ccagagacgg ctcgaacgca
      601 gtctcatcga gccttgacgt gccggtcacc atgtcgacag cttcgcctgc cgtctcaccc
      661 ggcgaggccc acaggcaaaa acggtgcgtg aaaggggcgt gacgccccgg gtggaggagg
      721 cacttctccc gcgcgacggc gcctgcccca cctcggccgg aacccggccg ggcttcgcgg
      781 gagcgcctcc gacgtaggga agaataggga tgcccgcgcc gcatgttcag ccgcgcggcg
      841 ccgcccgcga caaaggatcc ctatgaacgc ccttcctgcg caacccctga gcccgctcga
      901 cggtcgatac cgaccggtgg tcgaggggct cggcgtcgag ctctcagagg cgggcctcaa
      961 ccgggcgcgg ctgcgggtgg agacggagtg gctgatctac ctgaccgggc accgcatgtt
     1021 cggcagcacg ccgcttcgcg ccgacgatca ggcggccctg agccggctcg ccaccgactt
     1081 cgggcccgac gacatcgccg agctggctgc gctcgaggcc gagacccggc atgacgtgaa
     1141 ggcggtcgaa tacttcgtgc ggcggcgcct cgccgagcgc ggtctcgagt cgatcgccga
     1201 gctgacgcat ttcggggcga ccagcgaaga catcaacaac atcgcgtacg cgttgaccgt
     1261 gcgagaggcg gtcacgactg tctggctgcc ggccttcgac acggtcgtcc aggcgctgac
     1321 ggaacgggcc ctcgcctttc ggggcgacgc gatgctcgca cacacacacg ggcagccggc
     1381 gacgccgacg acgatgggca aggagctggc ggtcttcgtc taccggttgc gccgcatccg
     1441 cgggcagatc gtcgcgagcg actacctggc gaaattcagc ggggcgacgg gaactttcgc
     1501 ggccgacgtg gccgccgacc ccgacgtcga ctggcgtcag gtctcccgcg agttcgtcgg
     1561 ctccttcggc ctcacctgga acccgctcac gacgcagatc gagccccacg attggcaggc
     1621 cgaactgtac gcccggctga cgcacgcgaa ccgggtgctg cacaacctgg cgaccgactt
     1681 gtggacctac atctcgctgg gctacttccg tcaggtgccg caggcgggag cgacgggatc
     1741 ctcgacgatg ccgcacaaga tcaacccgat ccgattcgag aacgccgagg ccaacctgga
     1801 gctcagctgc gccgtgctcg acagcctctc ggcaaccttg gtgacaagcc gcatgcagcg
     1861 cgatctgacc gactcgacga cgcagcgcaa catcggcgtc gggctcggcc actcgctctt
     1921 ggccttggac aacctgcgcc gcggcctcga cgccctcgac ctggaccggg agcggctcgc
     1981 ttatgatctc gacgcgaact gggaggtgct gggcgaggcg atacagaccg tcatccgtgc
     2041 ggaagtcgtg gccggccgct cgcatatcgc cgacccgtac gcgctcttga agggcctcac
     2101 ccgcggcaaa cggatcggcc gggcggaact ggccgcgttc ctcgacggcc tcgagatcgg
     2161 cgacgctgcc cgagagcgtc ttcgggcgct gacgccggcc cgctacatcg gcctcgccga
     2221 aagcctcgtc gacgagctga cgaccgcccc ggccctctga cgccggacgc ggttcccgga
     2281 acagttccga gcgcaagggg cacggccgac ccggaactcc gataccggcg cggtcccccg
     2341 gcgcggcgga tgacaggacc ggccgacctc ggccgacaac cgggctttca cttttcactg
     2401 taacggcgtg agcaccacgg cccgcgggcg cccctagtga cccttgtctt cgcgatcttg
     2461 ctcttcgagc tcctcgacct cgtcggcctt cggctgaaaa ctcatggaaa gcagcgcgac
     2521 gatgacgagc acgatgatga acgagacggc taacgagatg ccggtgagcc accactcccg
     2581 ggtggctgcc aagacgatga tcgcggtgaa cacggccaga atcgccgaga agccgaccaa
     2641 ctcgaccggg cgcagccggt ctcggcgcgt cggttcgtag gccatgcccg gcagcggcat
     2701 gggtgtcggt gggatcttct tctcggcgcc gtcaccctgg ttgctcatct ggggcctttc
     2761 tgtcgcggct gtcttgctgc ccgcctgagc gggcctcgtc gacggcggcc catcgaagcg
     2821 agaggccgcc gatcacccag tacacacaga cgacggcggc gtatgcaccg aagattccca
     2881 caagcacgat ggcggcggtg agcgtgccgt gcacgccatc ctggccggcg aaatgcttgt
     2941 gcaggccggg cggcacgatg aggtatgcga ccgcgagtgc caaggagaga acgccgacgg
     3001 cccgccagtc tcgtgcggcc gcaaggcgtt tgcgagaccg cacgcccgca tacagttcca
     3061 gggcgccggt caagacggcc caggcgctga cgagggcgat aagcgcctcc agccccccgc
     3121 cgtgcagggc gagggctgcc gcgccgaaga ccaggccgag cgcgccgatg gcgacgaaaa
     3181 gggcgcgcac gatgccgcgt gggctggtgc gcagggcgag gacgccgacg agcacgccgg
     3241 ctgccacccc ataaccgccg aacaccagca gaccgacctg cgtggagtgg ttggcgagga
     3301 aggttatgat gacggccgcg acgaaggcga ccgacgcgcg cgcgatgggc atgacccaag
     3361 cacgttcacg caccgggccg gtgcggacaa cggccacctg gcgaccccat ttcgtatgta
     3421 tcggccgtca gtctaccgaa cccgtcccgg tgacggccga gtgcaggcct cttaaaacga
     3481 aaaccgaatg aggtccccca cgggctccgc ccgagaaccc ggtcagtcac gcaccatgtc
     3541 ggcgaagcgg gaatagtggc cttggaaggc gacggtgatg gtcctggtcg ggccgttgcg
     3601 atgcttggcg acgatgaggt ccgcctcgcc ggctcgcggg ttgtcgcgat cgtaggcgct
     3661 ctcgcggtgc aacaagatga cgacgtcggc gtcctgctcg atcgagcctg actcacgcag
     3721 gtcgctcaag gccggcagct tgtcttggcg ctgttccggg ccgcggttca gctgggagag
     3781 cgcgacgacc ggcacctgaa gttctttggc gagaagtttc aaggcgcggg agaattcgct
     3841 gacctcttgc tggcgggact cgacgcgttt gccgctcgtc atgagctgca gataatcgat
     3901 gacgaccatc ttgaggccga cgcgctgctt cagccggcga cacttcgccc ggatctcgac
     3961 gagcgtcatg ttcggactgt cgtcgatgta gagcggggcg tcgttgatac gaccgcgcgt
     4021 cgaggcgatc gtcgtccagt ctctgctttc gaccgtgccc ttgcgcatgc tttgcagcgg
     4081 cacggaagac tcggctgaga gcagccgcat cgcgatctcg ctgcgaccca tctcgagaga
     4141 gaagaggatc gtaggcagat cgtgtttgat cgcggccgac cgggcgaaat ccaacgcgag
     4201 cgtcgacttg ccgagcgccg ggcgtgcggc gacgatgatc atctgcccgg gatgcagccc
     4261 gttggtcaac tcgtcgaggt cggcgaaccc ggtcggcacg cccgtcatgg cgccgtcttt
     4321 gtgtcgggcc gactcgatct cgtcgatcgc ggcggtcacg gcctgcgtca gcggaacgta
     4381 gtcttcgctg tcgttcgaac cggtgacggc gtagatctcg gcctgcgcgt cgttcaccag
     4441 gtcgaccgcg tcgccctggc cggcgtagcc catctgcgcg atgcgggtgc ccgcatcgac
     4501 gagcctgcgc aggagcgccc gctcggcgac gatcgtcgcg taatagccgg cgttcgcggc
     4561 ggtcggcacg ataccggtca gggtgtgcag gtattcggcg ccgcccgccc gcgtgaggtt
     4621 gccctgcttc gtcaactcgt cggtgacggt gatgacgtcg gtcggctcgc cgtgcgagta
     4681 gaggtcgagg atcgcgccga agatgatctc gtgtttcggc acgtagaagt cggcgccgcg
     4741 caccgtctcg accacgtcgg cgaccgcgtc tttgctgagc atcatggcgc ccagcgtgct
     4801 ctgctccgac aggaggtcgt ggggcggcgt gcgctcttcg acgcccatcg ggtctgccgg
     4861 cggctcatcc agccgcccca acgacgctat cgacaccgtc tcacccccga acccgcagcc
     4921 acagcatgac accgaccacc gacatcacgg gccgtggcat gccccacagg ccaccgtaag
     4981 aagccggccc gccgccgaca attcaccctg tgaataactc tgtggaggaa atgcgggaaa
     5041 cgccgacgaa gctgtgtgga aagcctgtgg attactcccg gaaggggcac ggatttcttc
     5101 ctaaatacat actttgacca ggtcttttga tttacacaca ctttgtggat aaggaaccat
     5161 caaggacacc ttgaaccttg tcggcacgat ttctacgctg tgtacaaccg tggccgcgcc
     5221 ataccggccg ggcatgcgaa acggggccgg gcgaacacct cacgtgctcg cccggccccg
     5281 ttcgaccgtt cctgctattt cgaggcagcc acctcgatag tgatggtcgc cgtgatgtcg
     5341 tcccgcaggc gcagcagagc ctgatacgtg ccgaccgact tgatcggcac ggggatctcg
     5401 atcttgcgcc ggtcgacccc ggcaaggcct gcggcctcgg cggcggaggc gatgtcggct
     5461 gccttcacgg aaccgaacag gcgaccctcc cggcctgcct tcacgctgag cttcaagctc
     5521 gtcgcctcga ggcgcgcctt caaatcgagc gcctcctcga cggtcgcgtg ctccctggcc
     5581 aagcgtgccg ccttgatctg gtcgacctgc ttcgccccgc cgcgggtcca cgccacggcg
     5641 aaaccctgcg gtatcagata gttgcgggcg tacccggtct tcacgtcgac gacatcgccg
     5701 gcctggccga gcccggagac ctcgttcgtc agaatcaact tcgacatttc cgtgtctcct
     5761 tacctgccgg acccggcata gggaagaagc gccatctccc gcgcgttctt cacggcgcgg
     5821 gcgatgaggc gctgctcctg cacggagacg ccggtgatgc ggcgggccct aatcttgccg
     5881 cgttcagaga tgaacttgcg cagcgtcgcg acgtctttgt agtcgatcac gccgacgtgc
     5941 accgtgcggg ccggggcaac cggctttccg tctttgatct tgcctttgcg gttcttgcgg
     6001 cgatcgccgc tcgattttcc agccatgctg tttccttata tcgacaaaga ggacgattcc
     6061 ccggtcagaa cggggtctcg tcgttgaatg ttccgggtgt gttccacacg tcgccgccgg
     6121 acggcgcggc gggcgcgctg ggcgcccacg gctcgttgtc cgcggaggcg gcggggccgc
     6181 ggcccccgga ggacgaggtg cgggtgagcg tggcggtcgc gtaccgcagc gaggggccga
     6241 cctcgtccac ggtcaactcg atcacggtgc gcttctcgcc ttcgcgagtc tcgtagctgc
     6301 gctggcgcag cctgccctga acgatgacgc gggcgccctt cgtgagcgag ccggcgacgt
     6361 gctcggcgac gtcccgccac gcgctgcacc ggaggaacag agcctcgccg tctttccact
     6421 cgttgctcgc ccggtcgaac gtgcggggcg tcgaggccac ggtgaagctc gccaccgcga
     6481 cgcccgattg cgtgtaccgc agttcaggat cggcggtgag gttgccgatg acggttatga
     6541 cggtctcgcc agccatgacg actactccgc cttcgcaggc ctcagcgcgg ccgcgcggga
     6601 ggccttacgg gccgcacgct tcgccgcctg ggccacctgt gcgatggcct cttcggcccg
     6661 caggaccttg gtgcgcatga ccgcctcgga gagcttcaac tgccgttcca gctcttgcgt
     6721 cgcggcgggg ttcgccgtga agtcgacgac ggcgtaaatg ccctctgtct tcttctggat
     6781 cgggtaagcg agccggcgcc ggccccagac gtcgaccttc tcgatcgaac cgccatcgcc
     6841 gcggatcacg ccgaggaact tgtcgatgct gggcgcgacg gttcgttcgt cgatgccggg
     6901 atcgagaatg accatcagct cgtattgatg catgactgac ccacctcctt tggacttcgc
     6961 ggccgcagcc tttctgcggc aggagggtag tgcatcgtgc gcgcgagcat tgggcccggg
     7021 cgcacagcct caccagcata ccggaatgcc cggcgcggtc gcgcctgcgg cggtcccgtc
     7081 agtgggcgag tgccagcgcg gagacgagat gccgggcgag gtcctcggtg tcggccgccg
     7141 cgccgctgag atacgtggag cggcgacggc gcagaagact ggtgccgagc gtgtacaggc
     7201 cgtgtgcccg accgacgccg ccgtcatgca cgatgcgccc cttgcggtcg aaggtgtcga
     7261 catcgagcca cgagtagtcg ggcgcgaacc cggtggccca gacgaccgtc tcgatcccct
     7321 cccgcacaag gtcgaggctg aggcgcgggc gggcgggcac cgtgaccggg tcgggccggt
     7381 agggcgcctc ggtgatgtcg ccgtgctcct tcgcccactc gtcgaaacgg tcgagcaggc
     7441 gctcctcttt caggtcggcc aaccgggcga cgttcgcgag gccgccggag aagagcgccg
     7501 tgccgtcgcg caccatgccg agcttgccga cgatctccac gccgagttcc tgcagcgtgc
     7561 cgaggtcgag atcccggcgg gccttcgagc cgaccagctg gggggagggc acgtgcctgg
     7621 ctcggacgat gtcgtccatc tcgtcgtagc gttcatcgag cacgccggcc cgatccatcc
     7681 accagaacac gtcgcgcccg cggtaaacgc gaggcatccg caccagctcg ccgctggaga
     7741 cgacgacccg acggccgctg cgtgcgatct cgtccgccag ctgggctccg ctggcggagg
     7801 cccccacgac gagcacgccg cccgcgccga gctggtcggg gttgcggtag ctaagcgggg
     7861 tgatctggcg cacctcggcg gggatcgccg ccgccgcggc aggaaggttc ggccggcggg
     7921 ccgtgccgct cgccacgatc acgtgccggg cctgccacac gccgtcgtcg gtttcgaccc
     7981 ggtagccctc gtcggtcggt gtgaccttcg tcacggcggt atgagcacgc acaggtgcat
     8041 cgatgatctg agcataggag gagatgcggg agacgacctc cggcatgctc aggtagccgt
     8101 cgggatccgt gccgtcgtag tcgtagcccg gcaggccggt ctgccagttc ggcgtcaaga
     8161 ggtgcagcga atcccaccgt tcgctccgcc acgactgcgc gatctcgcca cgctcgaaga
     8221 tcacatggtc gacgccccgc gccgtcagac gagcgctcat ggcgaggccc gagtgccccg
     8281 cgccgacgat gacggcggtg atactgcgtt ctgtcacgaa tacctgttcc ggggcctcag
     8341 cccacgctca cgttgacggt ggtcgggttg gtgagcgcgt cgaagacggc ggaacgcttc
     8401 tgcgactgcg cgaccaaagc cgcgatctct tcctccgtcg catcggcatc gatgtcgaag
     8461 tgcacgtcga tctcgttgaa cccattgcgc acgtcggggt cggcgccgag gatgccgagg
     8521 atgttctctt tgccctcgag ggtcgccttg acggagcgca gctgaatctc acggcgctgc
     8581 gcgacggcgg cgataccggc ggtgaggcag ccggccagcg ccgcgagaac cgtctcgacg
     8641 ggggtggggg cctcgtcgtt gccggcgaag atgtcggggt ggtcgccctc cagctggaag
     8701 acgctcttgt gggcgcgctc ggtgccgagc ccggtgtact tctcgatggt ctgggtggaa
     8761 tgcgtgccgc tctgccattc cgaggtcgcc cgccagacga actcggcggc ctccggcgca
     8821 tcgcgaagct gctcgcgggc ggcgaccaaa gcatcactgc tgacgccgtt atcgaccggt
     8881 gcgtgcgggg tctgggtggt cgtcatggtt ctccttagat agggcggccc gcgctcggga
     8941 gtctgaaaac caggatattc gagccgggtg ccgcgcgcgg cttcgttacg ca
//
LOCUS       DASTNL010000009         8608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1492860, whole genome shotgun sequence.
ACCESSION   DASTNL010000009 DASTNL010000000
VERSION     DASTNL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492860"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFQ96_00300"
     CDS             complement(<1..734)
                     /locus_tag="VFQ96_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256259.1"
                     /translation="MRFVVAIVTFVVAAALIGLGLAQRTLFAPDPHITASVQLHGQPH
                     YVVIDGAVFGAHPGPQRVLLSGAGAADGFVAYGRTSDVVAWLSGEAYDRLQWGDTGEL
                     TARPVAAHRTVPGPAVSSPSAAAGDASAAGAAGAAGEARPDPAGSDLWLRSFSGPAAR
                     SVRLDVPRNVSVLIATDGTRPAPAAIQLSWPADTATPWTGPLVVAGLLLLLAGAIVYT
                     LALRHLHRSRRPRRRGRGGGGKMPRLP"
     gene            982..2727
                     /locus_tag="VFQ96_00305"
     CDS             982..2727
                     /locus_tag="VFQ96_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU0256260.1"
                     /translation="MAIDETEFTPAFRGYDRAEVDRTLQTLRQELIAANTQNAEYAKE
                     IRRLGAEIEGLQAQIDEVGNPTFAGLGTKLENTLRVAEEQATRLITQADIDAERLRSS
                     STAQADDTRAQARARAERTLSDAAVRAEATLEDARIEARNILADAEDRKEEILRQAGQ
                     EAVVTRGQAQTEAAALRTTAAREAAALKAEAERQATETTAAAEAEAEAARTDAASLAD
                     EASAARAALEQEVASGREAWERRLAEAEAEAEARLEQQERDQAAQAQAAHEGLENERD
                     EARREIAAAEASSRAKLRGEEAEAQADLATETEQARAALQAEVETTRANLAAEVAKAR
                     ADLAAEIEGSRAGWVARREEDEARFAADQAASRAAWDEELREARRALSQELAEARDDA
                     ERQLRDEAARARDEAARVRDEAEQAAEELQTHSRDEARQARTAVETERRRVTTELAAA
                     RDEAERDRLERQREASAQMQQYLEEARAELTRLQAEAEEARNEAATIRSAVREEAAQL
                     RREAEEKARATTVEAEAEAARKVAAAEERCRALVADAEDRLAEIRVEREAVAGYLENL
                     RGVLTQAENVAARHD"
     gene            2720..3808
                     /locus_tag="VFQ96_00310"
     CDS             2720..3808
                     /locus_tag="VFQ96_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0256261.1"
                     /translation="MTEPEPAQRRTIRLSGVFTAGLVGTLGVGLGALLLISVAHLSVI
                     ITYIGAALFLSLGLEPAVIFLSKLHFPRWSAILTVLVVVLGAITGIALAIVPVIVGQV
                     SQLIAQVPGIVASVESQNWIGILDKQFGAANVQSVIDSVTAFVTDPSKLTGIANGVLA
                     VGVSIVSGVFGSIIVAILTIYFTASMPAMKRGVALLVPASGRARFVDLSNQITRSVGR
                     YVMGQVALALINGILSFIFLSIIGAPFTAVLAFVAFLLSLIPLVGTLTGSIIIVATCL
                     IPGIGSSPATALAAAIYYIIYMQVEAYVLSPNIMNRAVQVPGVVVVIAALAGGSLLGI
                     LGALIAIPVAASVLLIIKEVVVPRQNAR"
     gene            complement(3855..4172)
                     /locus_tag="VFQ96_00315"
     CDS             complement(3855..4172)
                     /locus_tag="VFQ96_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256262.1"
                     /translation="MPRRNRPRKARGRGGPWARRGEAEAPGGQSARAGWSRVETRHGV
                     SWHVRPMTAERALKSYVCPGCGRAVEPGTGHLVVWRAQGVLGDAADLAGRRHWHAACW
                     RVS"
     gene            complement(4196..4876)
                     /locus_tag="VFQ96_00320"
     CDS             complement(4196..4876)
                     /locus_tag="VFQ96_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0256263.1"
                     /translation="MRKHSVTIVPLKSVPPPVTRRRLGTRLVLFVGSVCATLAFVLVN
                     IVDPYAGATAAPSFNAEMSLSGADGQHFVVAGVVDPPATAQDGFTVRRRIVAASIVRA
                     DPQAGASSPSPAQGSDGPPAAGTPSPGSAQAYARQLLQSQGMGEGQFACLVALWNRES
                     HWNTYAYNAGSGAYGIPQALPGSKMASVGADWKTNFKTQITWGLQYISRYGSPCGAWR
                     HSQATGWY"
     gene            complement(4925..5467)
                     /locus_tag="VFQ96_00325"
     CDS             complement(4925..5467)
                     /locus_tag="VFQ96_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU0256264.1"
                     /translation="MTTFRTVHGRRRGYDPDEVDGFLARARAAYNDESGVSLAAREVR
                     GTAFTLRRGGYETAEVDAALERLESALAARERESERLSLGDSAFREHIRLLWNDIAAR
                     LDRGDGRRFSRVSALSHGYRIREVDRLARQVSDYLTRSAPLGVETVRSSAFGAERRGY
                     REAEVDALLDAVVEAMLASG"
     gene            complement(5491..6489)
                     /locus_tag="VFQ96_00330"
     CDS             complement(5491..6489)
                     /locus_tag="VFQ96_00330"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0256265.1"
                     /translation="MRTLPPDGVGKAPPDDEGRDRPGARHPRFKAQFRATRLDLERQV
                     HETRAQFTATNERIQARTGRNLIGAILIGLGLGAVFLVSLLVVKELFIAVAAVILAVG
                     AFELSGALGHAGRRVPRIPAAAGGLAIAPAAYFGHDLGQLVCLAAAIVVIWAWRLAEA
                     LPAGHRRGGRALLLDLGAAVFVQVYVVFLGSFTVLLTARDGGQWWTIGFIVVVVAVDV
                     GAYASGLMFGRHPMAPKISPKKTWEGFEGAAVAALAAGVLLAVFALGRPWWFGLIFGA
                     ALLGSATVGDLIESLIKRDIGVKDMSSWLPGHGGFLDRLDSILPSALVAYFLFALS"
     gene            complement(6498..7055)
                     /gene="frr"
                     /locus_tag="VFQ96_00335"
     CDS             complement(6498..7055)
                     /gene="frr"
                     /locus_tag="VFQ96_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU0256266.1"
                     /translation="MIADVLAAAVERMAKSVESAKEDFATVRSGRADPRLFQRIPVDY
                     YGTQTPLGQLASLQVAEARMIVVTPYDKSAAKEIEKALVAGTQSLGANVGNDGQLLRV
                     TLPELTQERRKEFVKLVRQKGEDARVALRNIRRKAKDELDALTSEVGEDEVARAGKEL
                     ETATKKHVDLIDEALRNKESELLEV"
     gene            complement(7172..7888)
                     /gene="pyrH"
                     /locus_tag="VFQ96_00340"
     CDS             complement(7172..7888)
                     /gene="pyrH"
                     /locus_tag="VFQ96_00340"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0256267.1"
                     /translation="MSERAPRRRVLLKLSGEAFGAGRLGVDPDVVSSLARQIARAATD
                     VEIAVVVGGGNFFRGAELSQRGMERSRADYMGMLGTVMNALALQDFLEQAGAATRVQS
                     AIAMTQVAEPYIPRRAERHLEKGRVVIFGAGAGLTYFSTDTVAAQRALEIGADVVLVA
                     KNGVDGVYTADPNTDPTAQKIDHITYQEALRRGLKVVDSTAFSLCMDNNMPMQVFGMR
                     PAGNVTEAILGAPLGTLVSN"
     gene            complement(7977..>8608)
                     /gene="tsf"
                     /locus_tag="VFQ96_00345"
     CDS             complement(7977..>8608)
                     /gene="tsf"
                     /locus_tag="VFQ96_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0256268.1"
                     /translation="ADGVAYMIELACETDFVAKNSKFIDLADRVLAAVVAAGATDAQS
                     ALAAPAGQGTVADAIAEDAAVVGEKIALRRVAAVPGTAFSIYLHHTSKDLPPQVGVIL
                     AYQGDDAETARSIAQHISFADPRYLQQGDVPADEVENERRIVEEISRKEGKPEVALPR
                     IIEGRLHAYFKQIVLLEQDYARDNKLSVQQVVSDAGLTVTGFARFRVGA"
BASE COUNT         1376 a         3032 c         2998 g         1202 t
ORIGIN      
        1 ggcagccgcg gcatcttgcc ccctccgccc cggccgcgtc ggcgtggccg gcgagagcgg
       61 tggaggtgcc tcagcgcaag ggtgtagacg atcgcgccgg ccaagagaag gaggagcccg
      121 gccacgacca ggggcccggt ccacggcgtc gcggtgtcgg ccggccaact gagctggatc
      181 gcggcgggcg ccgggcgggt gccgtcggtc gcgatgagca cgctcacgtt gcgtggcacg
      241 tcgaggcgaa cgctgcgtgc ggctggccca gagaaggagc gcagccagag gtcgctgccg
      301 gccgggtcgg gccgggcctc gccggcagcc cctgcggcgc cggccgcgga ggcgtcgccc
      361 gccgcggcgg acggagaact caccgccggc ccgggcacgg tccgatgggc agcgaccggg
      421 cgggcggtga gctcgccggt gtcgccccac tgcaggcggt cgtacgcctc gccggaaagc
      481 catgccacga catccgaggt gcggccatag gcgacgaagc cgtcggcggc cccggccccg
      541 ctaagaagga cccgctgcgg tcccggatgc gcgccgaaca cggcgccgtc gatcacgacg
      601 taatgcggct ggccgtgcaa ctgcacgctc gccgtgatgt gcgggtctgg tgcgaacagc
      661 gtgcgctggg cgaggccaag accgatcagg gccgcggcaa cgacgaatgt gacgatcgcg
      721 acgacgaagc gcacctggtc cctttcccct cgccttctgg ctgccggccc gcccgcgccg
      781 actcacatat cgggtgcgtg cccggccggc gagccctggg aaccgccacc gcgacacgca
      841 aggtgtctaa gaatagcgga gagccctggg aggctcatgg gccggtcggg cacccgccga
      901 gactgggggc ggcacgccgt actggctaag atcgcgtgca gacccgtcgc atcgccggcg
      961 tcccgcgttc tggaggagca tttggccatt gacgagacag agttcacccc ggcgttccgc
     1021 ggctacgacc gggccgaagt ggaccggact ctgcagacgc tgcgtcaaga gctgatcgcc
     1081 gccaacacgc aaaacgccga gtacgcgaag gagatccggc gcctcggcgc cgagatcgag
     1141 ggtctccagg cacagatcga cgaggtgggc aatcccacgt tcgcggggct cggcacgaag
     1201 ctggagaaca ccctgcgcgt cgccgaggaa caagcgacca ggctcatcac ccaagccgac
     1261 atcgacgcgg agcggctgcg ctcgagctcc acggcgcagg ccgacgacac acgtgcccaa
     1321 gcgcgggcgc gggccgaacg aacgctctcc gacgccgcgg tccgcgcgga ggccactctc
     1381 gaagacgcgc gcatcgaggc gcgcaacatt ctcgccgacg ccgaggaccg caaagaggag
     1441 attctgcgcc aggccggcca ggaagcggtg gtgacccgcg gccaggcgca gaccgaggcg
     1501 gccgcgttgc gtaccacggc ggcgcgcgag gccgccgccc tgaaggccga ggcagaacgc
     1561 caggccaccg agacgacggc cgctgccgag gccgaggccg aggcggccag aaccgacgcg
     1621 gcgagcctcg ccgacgaggc gagcgcggcc cgcgcggcgc tcgagcagga ggtggcgtcg
     1681 ggccgcgagg cctgggaacg caggctcgcg gaggcggagg ccgaggcgga ggcacgcctg
     1741 gagcaacagg aacgcgacca ggccgcacag gcccaggccg cgcacgaggg tctggagaac
     1801 gaacgcgacg aggcccgtcg cgagatcgcc gcggccgagg cctcctcccg cgcgaagctt
     1861 cgcggcgaag aggccgaggc ccaggcagac ctcgccaccg agacagagca ggcccgcgcg
     1921 gccctgcagg ccgaggtcga gacgacccgg gcgaacctcg ccgccgaggt cgcgaaggcc
     1981 cgcgccgacc tcgcggccga gatcgagggt tcccgggccg gctgggtggc gcgcagggag
     2041 gaggacgagg ctcgcttcgc ggccgaccag gcggcttccc gtgccgcgtg ggacgaagag
     2101 cttcgagagg cccgccgcgc cctctcccaa gagctggccg aggcgcgcga cgacgccgaa
     2161 cggcagttgc gcgacgaggc cgccagggca cgcgacgagg cggcccgggt acgcgacgag
     2221 gcagagcaag ccgcagaaga gttgcagacc cacagccgtg acgaggcgag gcaggctcgc
     2281 accgccgtcg agacagaacg acgccgcgtg acgacagagc tcgcggcggc gcgcgacgag
     2341 gccgaacgag accgtctcga gcggcaacgc gaggcgtcgg cccagatgca gcaatacctg
     2401 gaagaggcgc gtgccgaact gacgcggctg caggccgagg ccgaggaggc caggaacgaa
     2461 gccgcgacga tacgctccgc cgtgcgggag gaggcggcgc agttgcgccg ggaggccgag
     2521 gagaaagcgc gagcgacgac ggtggaggcc gaggcggagg ccgcccgcaa ggtcgccgcc
     2581 gccgaagagc gctgccgcgc cctcgtagcc gatgccgagg atcgcctggc cgagatcagg
     2641 gtggagcgtg aggccgttgc gggctatctg gagaaccttc gcggcgttct cacccaggcc
     2701 gagaacgtcg ccgcccggca tgactgagcc agagccggcg caaagacgca cgatccgcct
     2761 ctccggggtg ttcaccgcag gcctcgtggg caccctcgga gtcggtctcg gcgcgctgct
     2821 cttgatctcc gtcgcacacc tctctgtcat catcacgtac atcggggccg cgctcttcct
     2881 ctcgctaggc ctcgagccgg ccgtgatctt cttgtcgaag ctgcactttc cgcgttggtc
     2941 ggcgattctc accgtgttgg tcgtcgtgct gggcgccatc acggggatag ccctcgcgat
     3001 cgtgcccgtg atcgtcggcc aggtctcgca gctgatcgcc caggtgccgg gcatcgtcgc
     3061 cagcgtggaa agccagaact ggatcggcat tctcgacaag cagttcgggg ccgccaacgt
     3121 gcagtcggtc atcgactcgg tcacagcgtt cgtgaccgac ccgtcgaagc tgaccggcat
     3181 cgccaacggc gtgctggccg tcggggtgtc gatcgtgagc ggcgtcttcg gcagcatcat
     3241 cgtcgccatc ctgacgatct acttcactgc ttcgatgccg gcgatgaagc gcggcgtggc
     3301 tcttctcgtg cctgcctccg gccgggcccg gttcgtggat ctcagcaacc agatcacccg
     3361 gtcggtcggc cgttacgtca tgggccaggt cgcgttggct ctcatcaacg gcatcttgag
     3421 cttcatcttc ttgtcgatca tcggcgcccc cttcacggcg gtgctcgctt tcgtcgcgtt
     3481 cctgctctcg ctcatcccgc tcgtcggcac gctgaccggg tcgatcatca tcgtcgcgac
     3541 ctgtctgata cccggcatcg gctcgtctcc cgcgacggcc ttggcggcgg cgatctacta
     3601 catcatctac atgcaggtgg aggcatacgt tctcagcccg aacatcatga accgcgctgt
     3661 gcaggtgccc ggcgtcgtcg tggtcatcgc cgcgctggcc ggagggtcgc tcctgggcat
     3721 cctcggggcg ctcatcgcca tcccggtggc cgcctccgtc cttctcatca tcaaggaggt
     3781 cgtagtgccg cgccagaacg cgcgatgaaa ggcaccccgg cgaagaaaac cgccaccctc
     3841 acccacgcgg agaatcaact gacccgccag cacgcggcat gccagtgacg tctgccggca
     3901 aggtctgccg cgtcgcccag cacgccctgt gcgcgccaca cgacgagatg gccggtaccc
     3961 ggttcgacgg cacgaccgca gccggggcag acgtacgact tcagcgcacg ctcggccgtc
     4021 ataggccgta catgccagga cacgccgtgc cgtgtctcga cgcggctcca ccccgcgcgc
     4081 gccgactgcc cgcccggcgc ctcggcctcc cctcgccgcg cccagggccc gcccctgcca
     4141 cgcgcctttc tcgggcggtt ccggcgcggc atgctcccag cctaggccgc ccgggtcaat
     4201 accagccggt cgcctgagaa tgccgccagg ccccgcacgg gctgccgtac ctgctgatgt
     4261 actgcaggcc ccacgtgatc tgcgtcttga agttcgtctt ccagtcggcg cccaccgagg
     4321 ccatcttgct gcccggcaac gcctgaggaa tgccgtaggc cccgctgccg gcgttgtagg
     4381 cgtaggtgtt ccaatgcgac tcccgattcc acagggcgac gaggcaggcg aactggccct
     4441 cccccatgcc ttgcgactgc aggagctgac gcgcgtacgc ctgggcgctg cctggcgacg
     4501 gagtgcctgc cgccggcggg ccgtcagaac cctgtgccgg cgagggagaa gacgcacccg
     4561 cctgcgggtc ggcacgcacg atcgaggccg ccacgatgcg ccgacgcacc gtgaaaccgt
     4621 cttgcgccgt ggcgggcgga tcgacgacgc ccgcgacgac gaagtgctgg ccatccgccc
     4681 cgctgaggct catctcggcg ttgaagctcg gtgccgcggt cgcccccgca tacgggtcga
     4741 cgatgttgac gagcacgaac gcgagggtgg cgcagaccga gcctacgaac aggacgagcc
     4801 gggtgccgag gcgcctccgg gtgacgggcg gtggaaccga cttgagcgga acgatcgtta
     4861 ccgaatgttt acgcacaata gtgaaaccat agcgtaaggc ctattcgagg caaaaggcga
     4921 agggtcaccc ggaggcgagc atcgcctcca ccacggcgtc gagaagcgcg tcgacctcgg
     4981 cctcccgata accgcggcgc tccgccccga aggcggacga acgcaccgtc tcgacgccga
     5041 gcggggcgga acgcgtcagg tagtcggaga cctgccgtgc gaggcggtct acctcgcgta
     5101 tccggtaccc gtggctcagg gcactcacgc gggagaaccg ccggccgtcg ccccggtcga
     5161 ggcgagccgc gatgtcgttc cacagcagcc tgatgtgctc gcggaaggcc gaatcgccaa
     5221 ggctcagccg ctcgctttcc cgctcccggg ccgccagagc gctctccaac cgttccaagg
     5281 ccgcgtcgac ctcggccgtc tcgtagccgc cgcggcgcaa cgtgaaggcg gtgccccgca
     5341 cctctcgggc ggcgaggctc acgccggact cgtcgttgta ggcggcgcgg gcgcgggcga
     5401 gaaaaccatc gacctcgtcc gggtcgtagc cccgccgtcg gccgtgcacg gtgcggaatg
     5461 tcgtcactcg atacagtgtg gcagagccca ttatgacaac gcgaacagaa agtaggcgac
     5521 gagcgccgag ggcaggatcg agtcgagccg gtcgaggaag ccgccgtggc ccggaagcca
     5581 agaactcatg tctttgaccc cgatgtcgcg tttgatcaat gactcgatga ggtcgccgac
     5641 cgtcgcgctg ccaaggaggg cggcaccgaa gatcaacccg aaccaccacg gccgcccaag
     5701 ggcgaacacg gcgaggagca cgccggccgc gagcgcggcc accgccgcac cctcgaaacc
     5761 ttcccacgtc ttcttcgggc tgatcttcgg cgccatcggg tgccggccga acatcagccc
     5821 gctggcgtag gcgcccacgt cgacggcgac gaccaccacg atgaacccga tcgtccacca
     5881 ctggccgccg tctcgggccg tcagaagcac ggtgaagctg ccgaggaaga ccacgtagac
     5941 ctggacgaac acggcggcgc cgaggtcgag gagaagggcg cggccgccac gccggtgccc
     6001 ggccggaagg gcctcggcca agcgccacgc ccatatgacg acgatggcgg ccgcgaggca
     6061 gacaagctgg ccgaggtcgt ggccgaaata ggccgccgga gcgatcgcga gcccaccggc
     6121 cgcggcaggg atgcgcggca ccctgcgacc cgcgtgcccg agagcccccg aaagctcgaa
     6181 cgcccccacg gcgagaatca cggcggcgac ggcgatgaac agttccttca cgaccaggag
     6241 gctgacgagg aacaccgcgc cgagccccag gccgatcagg atggcgccga tcaggttacg
     6301 gccggtgcgc gcctggatgc gctcgttggt ggcggtgaac tgtgccctgg tctcgtgcac
     6361 ctggcgttcg aggtcgagcc gtgtggcgcg gaactgagcc ttgaagcggg ggtggcgcgc
     6421 gcccggccgg tccctcccct cgtcgtcggg cggggccttt cccacgccgt cgggcgggag
     6481 ggttctcatc ggcctcctca cacttcgagc agttcggatt ccttgttacg cagcgcctcg
     6541 tcgatgaggt cgacatgctt cttcgtggcg gtctcgagtt ctttgccggc ccgggcgacc
     6601 tcgtcctcac cgacctcgga ggtgagcgcg tcgagctcgt ccttggcctt gcgccgaatg
     6661 ttgcgcaggg ccacccgcgc gtcctcgccc ttttgccgca cgagtttcac gaactctttg
     6721 cgccgctcct gggtgagctc cggcagcgtg acgcgaagaa gctgcccgtc gttgccgacg
     6781 ttcgcgccga gactctgggt gcccgcgacg agcgccttct cgatctcttt cgcggccgac
     6841 ttgtcgtacg gcgtcacgac gatcatgcgc gcctccgcga cctgcagcga ggcgagctgg
     6901 ccgagcgggg tctgggttcc gtagtagtcg acggggatgc gttggaagag ccgcgggtcg
     6961 gcgcggccgc tgcgcaccgt cgcgaagtct tccttcgccg actcgacgga tttcgccatc
     7021 cgttcgactg ccgccgccaa aacatccgca atcacggaac cctccacact ccccggtcgt
     7081 tcgcctgccg cccgaacctg ccacgccgac cggaccgtgc gtggccgcgc accgtcagtc
     7141 tatggcctgc acaccggcgc gtccggcggg atcagttgga gacgagcgtt ccgagcggcg
     7201 ccccgaggat ggcctcggtg acgttgccgg ccggacgcat gccgaagacc tgcatgggca
     7261 tgttgttgtc catgcacagg ctgaacgccg tggaatcgac gaccttcagg ccgcgccgca
     7321 aggcctcctg atacgtgatg tggtcgatct tctgcgcggt cggatccgtg ttcgggtcgg
     7381 cggtgtagac gccgtcgacg ccgttcttcg cgacgagcac gacatccgcc ccgatctcca
     7441 acgcgcgctg cgccgcgacg gtgtcggtcg agaaataggt gagcccggcg ccggcgccga
     7501 agatgacgac acgccccttc tccaggtgtc gttccgcacg gcgtggaata tagggctcgg
     7561 cgacctgcgt catggcgatc gcggactgca cgcgcgtggc ggcgcccgcc tgctcgagga
     7621 agtcttgcaa cgcgagcgcg ttcatgacgg tgccgagcat gcccatgtag tcggcccggc
     7681 tgcgctccat gccgcgttgg ctgagctccg cgccacgaaa gaagttgccg ccgccgacca
     7741 cgaccgcgat ctcgacgtcg gtggcggccc gcgcaatctg ccgcgcgagc gagctcacca
     7801 cgtcggggtc gacgcccagc cgaccggcgc cgaacgcctc accggaaagc ttcaagagaa
     7861 ccctgcgccg aggcgcccgc tcactcaccg tgccgtctcc cgtcgtcgtc gcccccgcga
     7921 tgcggcactg cgccgcgccg gcccgcgaag ctgcccgggc cggcgatgcc ccggtgtcag
     7981 gcgcccaccc ggaaccgggc gaacccggtc acggtgaggc ccgcgtcaga gacaacctgc
     8041 tgcacggaga gcttgttatc gcgcgcatag tcttgctcga gcagcacgat ctgcttgaag
     8101 tacgcgtgca gccgcccctc gatgatgcgc ggcagagcga cctccggctt gccttctttg
     8161 cgggagatct cctcgacgat gcgccgttcg ttctcgacct cgtcggccgg cacgtcgcct
     8221 tgctgcaggt accgcgggtc ggcgaacgag atgtgctgcg cgatgctgcg ggccgtctcg
     8281 gcgtcgtctc cctgataggc gaggatgacg ccgacttgcg gcgggaggtc cttcgaggtg
     8341 tggtgcaggt agatcgagaa cgccgtgccc ggcaccgcgg ccacgcgacg cagcgcgatc
     8401 ttctcgccga cgacggccgc atcctcggcg atcgcgtcgg ccaccgtgcc ctggccggcg
     8461 ggagcggcca gggcgctttg cgcatccgtg gcgcctgcgg cgacgaccgc ggcgaggacc
     8521 cggtcggcca ggtcgatgaa cttgctgttc ttcgcgacga agtcggtctc gcatgcgagc
     8581 tcgatcatgt aggcgacgcc gtcggcct
//
LOCUS       DASTNL010000010         5556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_936923, whole genome shotgun sequence.
ACCESSION   DASTNL010000010 DASTNL010000000
VERSION     DASTNL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936923"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFQ96_00350"
     CDS             complement(<1..308)
                     /locus_tag="VFQ96_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0256269.1"
                     /translation="MTKDDAFAADLIRGHTDTIVLGMLRHHDRYGFEIYKAIREAVGG
                     GHEIKEATLYATYRRLQRDGLVESYWGDETQGGRRKYYRITDAGRAVYKGNVAAWVAT
                     R"
     gene            complement(558..944)
                     /locus_tag="VFQ96_00355"
     CDS             complement(558..944)
                     /locus_tag="VFQ96_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256270.1"
                     /translation="MSDEPVVRGLGREIPRSLGGILAAIVLIVAIGLFVWSLIATWSG
                     QQQDGLPLFPVMIGGGVVTTLAFYGYRTVSWIVALVAVACWILVTALPIIPVVEALLQ
                     AVTVTAMVVGINITLEAVKYLKTPRF"
     gene            complement(1067..1780)
                     /locus_tag="VFQ96_00360"
     CDS             complement(1067..1780)
                     /locus_tag="VFQ96_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0256271.1"
                     /translation="MRVISYNLRKNHARSELVGLLMQFHPDVLCLQECDPTDLPADLA
                     GLHLADSTRHNRLGLAVYYDDERFPRQETRTFTLKKSLHDRLLSPAYERLVGTRLKEA
                     HGGVSFVVASFHAAPLTASNSLRRKQITAAYGNLRALGQGAPTLMVGDYNYPIFQDRL
                     VAHVGASGYELSLSDTRTYTRYKVFRGHFDFVTSTGFTVGNVETLPGGDSDHRPILVD
                     AQRTGLSNAAPQAPADVPA"
     gene            complement(2035..5520)
                     /locus_tag="VFQ96_00365"
     CDS             complement(2035..5520)
                     /locus_tag="VFQ96_00365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEU0256272.1"
                     /translation="MGWNNPPIPWAQLERTLSGRGRRVDGEKSSPTRGAEPSGGDDGG
                     GRAGSPSSPRAPVPYAELHAHSNYSFLDGASSPEELLRGAARLGLTALALTDHDGLYG
                     VVRMAEAANALRRRHPDGPTPRTVFGAELSLGMSGRQGGVADPEGDHLLVLARGRAGY
                     RRLAHALTEGHLNGDAAKGSPRYDLERLAGTARGDWVILTGCRKGSVRRVLEEATRRG
                     DDLTEIRRAVGAGLDRLTQLFGQENVFVELIDHGHPGDEARNDILAAVAHDQGLPLLA
                     SNNVHYATPVEHRLAQTVAAVRSRRSLDELDGWLPAAGTAHLRSGAEQAARFARYPGA
                     VENTVAVAEATAFELRSLNPRLPAVGVPAGHTPMSWLRELVRCGVAERYPAAPREEAA
                     VGDSVWARLHRELDVIEAKDFPGYFLIMHDIVRFARDKGILCQGRGSAANSAVCYALG
                     ITAVDSIFYGLPFERFLSSIRDDEPDIDIDFDSGRREEVIQYVYGRYGRRSAAQVADV
                     ISYRPRSAVRDVAKALGYGPGLQDAWSAGVGRSRTERDGDAPTRAPGIPPLVEDLSGR
                     LLKAPRHLGIHSGGMVLTRRPVGDICPIERAAMPGRTVLQWDKEDCAWMGLVKFDLLG
                     LGMLAALRHVLDLVTDPLGESWSLASIPREEPAVYDMLCRADTIGVFQVESRAQMGML
                     PRLRPRSFYDLAIQIALIRPGPIQGGAVHPYIRRRTGGEAVTYLHPALEPVLRRTLGV
                     PLFQEQLMQMAMAVGGCDAEDADLLRRAMGSKRGVEHVETLREKLYEGMAAHGISEDD
                     ADAIYAKIEAFASFGFAESHSLAFAHLVYASSWLKLHYPAAFLAALLRAQPMGFYAPH
                     TLVDDARRHGVRVLPVDVLHSDVDAGLEPIRSGGRAKTPRAGGMPGCLDHDQPPVGPF
                     EPGEHPADATHRRDGAYAVRLGLTAVREVGRPHAERIVEERRRGPYRDMNDLSRRAGL
                     TAAQLESLAAAGALAGFGLSRRAALWSAGPAAQESLDRLAGSAVTVQPPLLPLLTDEE
                     RTAFDLWATGVSGQHPLQHLRASLTARGVVSTAEARVVPSGYRVEVAGAVTHRQRPRT
                     AGGITFLNLEDETGILNVVVRPGVWKRFQEAAASPAMLVRGVMERSVEGVSAMLADRI
                     QDLRLSAPLVSRDFR"
BASE COUNT          920 a         1886 c         1953 g          797 t
ORIGIN      
        1 cgggtcgcga cccaggcggc gacattcccc ttgtaaacgg cacgccccgc atcggtgatg
       61 cggtaatact tacggcgccc gccctgcgtc tcgtcacccc aataggactc gacaaggccg
      121 tcgcgctgca agcgccggta ggtcgcgtaa agggtcgcct ccttgatctc gtggccgccc
      181 cccacggcct ctcggatcgc cttgtagatc tcgaagccgt aacggtcgtg gtggcgaagc
      241 atgccgagca cgatcgtgtc ggtgtgccca cggatcagat ccgcagcaaa ggcatcgtcc
      301 ttcgtcacat cgggtactat atcacgcata gtattattca cagtagaaat accgatgtgc
      361 cactcgctgc ggacgggtgg ccgtggagag agggccacgg caagaaccgc gaaggggtgc
      421 gcgccatgcc ctcaaccccg gcagacgaac gctggagggc cgcccgaaag gggcccgtca
      481 ccgggtgcgg cgagaagaat cctccgcgac gaagacgcgc gaaacgaatc ctcggcgaga
      541 ggcgccgaag ccgggaatca gaagcgcggg gtcttcaggt acttgacggc ctcaagcgtg
      601 atgttgatgc cgaccaccat cgcggtgacg gtcaccgcct gaagcagtgc ctcgaccacg
      661 ggaatgatcg gcagtgcagt gacgaggatc cagcaggcga ccgcgacgag agcgacgatc
      721 cagctgacgg tgcgatagcc gtagaaggcc agcgtggtga caacgccgcc gccgatcatg
      781 acggggaaga gcggcaggcc gtcttgctgc tggcccgacc aggtagcgat gaggctccag
      841 acgaacagcc cgatggcgac gatgagcacg atcgccgcaa ggatcccgcc cagggagcgg
      901 ggaatctcgc ggccgagacc gcgcacaacc ggttcgtcgc tcaccgctgg gttctgttcg
      961 actgttgtct cggcgctcgt cacgacaaca tcttagccgg tggccgccgg gcggctcacg
     1021 ccgaccgagg ataaaacgcg gctccgcccc acgcctggaa caagggctat gcaggcacgt
     1081 ctgccggcgc ctgcggcgcg gcgttcgaaa gcccggtacg ttgcgcgtcg acgagaatcg
     1141 gccggtggtc ggagtcgccg ccgggaaggg tctcgacatt gcccacggtg aaccccgtcg
     1201 aggtcacgaa gtcgaagtgg ccacgaaaga ccttgtaccg cgtataggtt ctcgtgtcgc
     1261 tcaaagagag ctcatagccc gaagcgccga cgtgcgccac gagcctgtct tgaaagatcg
     1321 ggtagttata gtcgccgacc atgagcgtcg gggcaccctg accgagggcc cgcaggttgc
     1381 cgtaggcggc cgtgatctgc ttgcggcgca acgagttcga cgccgtcaac ggcgcggcat
     1441 gaaaagacgc gacgacgaac gagacgccac cgtgcgcctc tttcagccgg gtgccgacca
     1501 gcctctcgta ggcgggggag aggagtcggt catgtagcga cttcttcaag gtgaaggtgc
     1561 gggtctcctg gcgaggaaaa cgttcgtcgt cgtagtacac cgccagcccc agcctgttgt
     1621 gtctcgtcga gtcggccagg tgcaggcccg ccaggtcggc gggtaggtct gtcgggtcac
     1681 actcctgcag gcagagcacg tcggggtgga actgcatgag aaggccgacg agctcgctgc
     1741 gggcatgatt ttttcgcaag ttgtagctaa tgacgcgaat cacggcgacc gctttctggg
     1801 cgagacgaac gcgcccgctc cccagagcat agggcgtgtg tctgagacag gtcgggagaa
     1861 acacacagcc acgcgccaca cccagctccg cccacccgag ggccgccgcc ggcaaggtgc
     1921 tttcccggca tcgcatcgcc gcccggcccc gcccgcctga gggccgccga aagaagagag
     1981 ggggcgagcg ccgcgtcgca gagggacaga gacagagagt accgagggct cgtgtcaccg
     2041 gaaatcgcgg gagacgagcg gggccgacag acggaggtct tggatgcggt cggcgagcat
     2101 cgcgctgacc ccctccacag agcgctccat gacgccgcgt acgagcatcg cgggggatgc
     2161 cgcggcctcc tggaacctct tccacacgcc gggccggacg acgacgttca ggatcccggt
     2221 ctcgtcttcc aaattcaaga aggtgatccc gccggccgtc ctcggccgct gccggtgggt
     2281 gaccgccccg gccacctcga cacggtagcc cgagggcacg acacgggcct cggcggtact
     2341 cacgacgccc cgcgcggtga gcgaggcccg aaggtgctgc agggggtgct gcccggagac
     2401 cccggtggcc cacaggtcga aggcggtccg ctcctcgtcg gtgaggagcg gcaagagcgg
     2461 cggctgcacc gtcaccgcgc tgccggccag gcggtcgaga ctctcctgcg cggccggacc
     2521 cgccgaccac agggccgccc ggcgggagag gccgaagccg gcgagcgcgc ccgcggcggc
     2581 cagcgactcc agctgcgccg cggtcagccc tgcccggcgg gagaggtcgt tcatgtctcg
     2641 atatggtccc cgccgccgct cctcgacgat ccgctcagca tgggggcgac cgacctcccg
     2701 caccgcggta agacccagcc gcaccgcgta cgccccgtct ctgcggtggg tggcatccgc
     2761 cgggtgctcg cccggctcga acgggccgac cggcggctgg tcgtggtcga gacagccggg
     2821 catgccgccc gcccgcgggg tcttcgcccg gccgcccgat cggatcggct cgagccccgc
     2881 atccacgtcg gaatgcagga cgtcgacggg aagcacccgg accccgtggc gccgagcgtc
     2941 gtccaccagc gtgtgcgggg catagaaccc catcggctgg gcgcgcaaca acgcggccag
     3001 gaacgcggcg gggtagtgca gcttcagcca cgagctggcg tagaccaggt gagcgaaggc
     3061 gagagaatgg ctctcggcga agccgaagct ggcgaacgcc tcgatcttcg catagatcgc
     3121 gtcggcgtcg tcctcgctga tgccgtgcgc cgccataccc tcatacagct tctcgcgcag
     3181 cgtctccacg tgctccacgc cgcgtttcga gcccatcgcg cggcgcagaa ggtcagcgtc
     3241 ttccgcgtcg cacccaccga ccgccatcgc catctgcatg agctgctctt gaaagagcgg
     3301 caccccgagg gtgcggcgca gcaccggttc caacgccggg tgcagatagg tgaccgcctc
     3361 gccgcccgta cgacggcgga tgtacggatg cacggcgccg ccctggatgg ggcccgggcg
     3421 gatgagggcg atttggatgg cgaggtcgta gaagctgcgg ggacgcagcc tcggcagcat
     3481 ccccatctgc gcacggctct ccacctggaa gacgccgatc gtgtcggctc ggcacagcat
     3541 gtcgtacacc gccggctcct cgcgcgggat gctggcgagg ctccagctct ccccgagcgg
     3601 gtccgtgacc aggtcgagca cgtgccgcag ggcggcgagc atgccgaggc cgagaaggtc
     3661 gaatttgacg aggcccatcc aggcgcagtc ttccttgtcc cattgcaaga cggtacgacc
     3721 cggcatggcg gcacgctcga tggggcagat gtcgccgacc gggcggcggg tgagcaccat
     3781 gccgccggag tggatgccga ggtgcctcgg tgctttcagc agccggccgg agaggtcttc
     3841 gacaagcggc ggtatccccg gtgccctcgt gggcgcgtcg ccgtcacgct ccgtccgaga
     3901 acggccgacc ccggccgacc aggcgtcttg cagcccgggg ccgtagccga gcgccttcgc
     3961 cacgtcgcgc accgcggagc gcggccggta gctgatcacg tcggcgacct gggcggcgct
     4021 ccgccgaccg tagcggccgt agacgtattg gatgacctcc tcacgccggc cggagtcgaa
     4081 gtcgatgtcg atgtcgggct cgtcgtcgcg gatgctggag aggaagcgtt cgaagggcag
     4141 cccgtagaag atcgagtcga cggcggtgat gccgagcgcg taacagacgg cggagttcgc
     4201 ggccgagccg cgaccctggc agaggatgcc cttgtctcgg gcgaagcgca cgatgtcatg
     4261 catgatgagg aagtagccgg ggaagtcctt ggcctcgatg acgtctagtt ccctgtgcag
     4321 gcgggcccag acggagtccc cgacggcggc ctcttcgcgc ggcgcggccg gatagcgttc
     4381 ggcgacgccg caacgcacga gctctcgcaa ccacgacatg ggggtgtgcc cggcgggcac
     4441 accgaccgcc ggcagcctgg ggttcaggga acgcagctcg aacgcggtcg cttcggcgac
     4501 ggcgaccgta ttctccacgg ctccggggta ccgggcgaag cgggcggcct gctcggcccc
     4561 actgcgcaag tgggccgtgc cggcggccgg cagccagccg tcgagctcgt cgaggctgcg
     4621 acgggaccgc acggccgcca ccgtctgcgc caggcggtgc tcgaccggcg tcgcgtaatg
     4681 cacgttgttg ctggcgagaa ggggcaggcc ctggtcgtga gcgacggccg cgaggatgtc
     4741 gttgcgcgcc tcgtcgcccg ggtggccgtg gtcgatcagt tcgacgaaga cgttctcctg
     4801 gccgaagagc tgggtgaggc ggtctagccc cgcgccaacc gcacgccgaa tctcggtcag
     4861 gtcgtcgcct cgacgcgtcg cctcctcgag cacccgccgc acggaacctt tgcggcagcc
     4921 ggtcaggatg acccagtcgc cccgggccgt gccggccaat cgttcgaggt cgtagcgcgg
     4981 gctgcccttc gcagcatcgc cgttcagatg gccttccgtc agggcgtgcg ccagccggcg
     5041 atagccggcc cggccgcggg caagcacgag gaggtggtcg ccttccgggt cggcgacgcc
     5101 gccctgccgc ccggacatgc ccaggctgag ctcggctccg aagacggtgc gtggggtcgg
     5161 gccgtccggg tgccgccgac gaagggcgtt ggcggcctcc gccatgcgca cgaccccgta
     5221 cagcccgtca tggtcggtca aggcgagcgc ggtgaggccc agcctggcgg ccccgcgtag
     5281 gagctcctcg ggggaggaag ccccatcgag aaagctgtag ttcgaatgcg cgtgcagctc
     5341 ggcgtagggc accggcgcac gcggcgagct agggctcccc gcccgcccac cgccgtcatc
     5401 accgccggaa ggttccgctc cccgtgtcgg cgaagacttt tcgccgtcga cccgacgtcc
     5461 gcgcccggag agtgtgcgtt ccaactgcgc ccaggggatc ggcgggttat tccaccccac
     5521 gaggccgccc tttccgggcc cacaccggcc ttcttc
//
LOCUS       DASTNL010000011         6122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_382573, whole genome shotgun sequence.
ACCESSION   DASTNL010000011 DASTNL010000000
VERSION     DASTNL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382573"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFQ96_00370"
     CDS             <1..783
                     /locus_tag="VFQ96_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0256273.1"
                     /translation="KTELAKALAEAMFGDEQAMVRFDMSEFGERHTVSRLVGAPPGYV
                     GYDEAGQLTERVRRRPYTVVLFDEIEKAHPDVFNLLLQVLDDGRLTDGQGRTVDFRNT
                     VIIMTSNIGSEFLASRSGALGFVASDTAGGFGSDKELRDRVMGRLREAVRPEFLNRID
                     EIVLFRALEKPQLEEIVRMMLRTTAARLSEQEVTLDVSEEAVGWIAEHGYQREYGARP
                     LRRLIQREVEDRVAALLIEDGLHAGQHAVVEVSGDELRVEAR"
     gene            968..2098
                     /locus_tag="VFQ96_00375"
     CDS             968..2098
                     /locus_tag="VFQ96_00375"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU0256274.1"
                     /translation="MVSGVVGPVLARLALGLAHAQAGSGTSGSGDTTGFGGMTGMAGM
                     GGPDMHGDMWHPTLYPTTARIVAIHLQPIPILPALSVAALLAYLAGVWILRRRGTHWP
                     VLRTLWWVIGIATVLAMTATAVDGYGMELFSVHMVQHMVLTMLTPIFLTLGAPLTMLI
                     RVLPPGSGPFNARRLLLLLLHSRFAQFVTHPVVTLIVFLMSLYGLYFTPVFDWLMSSM
                     WGHNLMLAHFVLIGMLYFWGIMGVDPSPRRYNNQLRRFNAEVVRIFEIFVTVPFHAFF
                     GIVVIMSTSLIVGFYANPMKGWGISPLSDQRTAGGIAWGFTEIPTVVLLSALVMIWQR
                     SEKRHEKAIDHQAELNHDADLVAYNAYLAKVSGQDAATPPRR"
     gene            complement(2119..2748)
                     /locus_tag="VFQ96_00380"
     CDS             complement(2119..2748)
                     /locus_tag="VFQ96_00380"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU0256275.1"
                     /translation="MARLTAGVDAGISSDLVTGDDGKARPAWAIRDPLLQGYYDTEWG
                     MPVTDEAGLLERIILEGFQAGLSWLTILRKRAALRLAFQGFDADAVAAFGEPDVERLM
                     ADAAIIRNRRKIGAAITNARATVALRDEGGLAKLIWAHRPATTPHPRRLADVPTRSAE
                     SEALARDLRGHGFVFVGPTTMFAMMEAIGVIDTHLVGSHRRGTSGIWPD"
     gene            complement(2750..3745)
                     /locus_tag="VFQ96_00385"
     CDS             complement(2750..3745)
                     /locus_tag="VFQ96_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEU0256276.1"
                     /translation="MTDDNLMHAVTIAAPGGPEALTLTTVPRPRPGEREVLIKVAAAG
                     VNGADLMQRRGHYPPPPGAPEWLGLEVSGRVVAVGANTSRADIGDEVCALLSGGGYAE
                     YVSVHESLVLPVPHGVSLRDAAALPEALVTAWSNIRLFAGLKAGQSLLVHGGSSGVGL
                     AAIQIARAHGARVVATVGSEEKAAFCRRFGADAVNYRQADFVSEVGRLTAGAGVDVVL
                     DMVGGDYIARDLAVLATGGHIMLIADRTGTPASFPAGLLIAKRARLQGTAVRPRPLDE
                     RAALLAGLQAEVWPMVDDGRVRVVVDSVYPLAAAADAHRRMESSAHMGKILLAVD"
     gene            complement(3901..5325)
                     /gene="merA"
                     /locus_tag="VFQ96_00390"
     CDS             complement(3901..5325)
                     /gene="merA"
                     /locus_tag="VFQ96_00390"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEU0256277.1"
                     /translation="MQNTYDLAIIGSGAAAFAAAARAVGLGKSVVMVERGTIGGSCVN
                     TGCVPSKALIAAAEARHTAAEATARFPGITTSASPADMPALIDGKQDLVDAQRAEKYV
                     DIADFYGWDMVPGDAAFAGTPDVPTLRVASREGNVATIEADAYLVATGSRAWIPPIEG
                     LEKAGYLTSTTAMELTELPESLLVLGGGYVALEQAQLFARLGSKVTLLARSRLASREE
                     PEVAQTLQKVFADEDIRVLRRAVALRVAREAPDGQAVVTAEVAGRTQEFPTDRVLVAL
                     GRRPATCGLALDTVGVETGEAGEVVVTDRLRSSNPRIWAAGDVTGHPQFVYVAAREGA
                     VAAGNALAGADEHMAFTGLPRVIFTSPAVGTVGLTEKETVAAGIRCTCSVLPLSHVPR
                     ALVNRDIRGFIKIVANVDTGEIVGLTAVSEGAGELAAEGVHLLGKSITEVADAWVPYL
                     TMAEGIRIAAKAFTTDVSKLSCCA"
     gene            5429..5818
                     /locus_tag="VFQ96_00395"
     CDS             5429..5818
                     /locus_tag="VFQ96_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEU0256278.1"
                     /translation="MRIGELADAGGTTTATLRFYEEQGLLPSPGRTPGGYRDYAPDAV
                     TRVDFIHRGQAAGLTLAQIGQILDIRDQGLAPCRHVRDLLDTRLAEIDAQLARLAGLR
                     ETIAALRDGADHLEPGTCSPDRVCRYL"
     gene            complement(5905..>6122)
                     /locus_tag="VFQ96_00400"
     CDS             complement(5905..>6122)
                     /locus_tag="VFQ96_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0256279.1"
                     /translation="YGDMWAYMMLAEGLLDIVGEYDVKTYDLAALIPIVEEAGGRFTA
                     LDGTEGPSHGSALATNGLLHSAVLAAL"
BASE COUNT          940 a         2018 c         2120 g         1044 t
ORIGIN      
        1 aagacggaac ttgccaaggc attggccgag gcgatgttcg gcgacgagca ggcgatggtg
       61 cgcttcgaca tgagcgagtt cggcgaacgc cacaccgtca gccgtctggt gggcgcacct
      121 ccgggatacg tcggctacga cgaggccggc cagctcaccg agcgggtgcg tcgccgccct
      181 tacaccgtgg tgttgttcga cgagatcgag aaggcgcatc ccgacgtctt caaccttctg
      241 cttcaggtgt tggacgacgg tcggctgacc gacgggcagg gccgcacggt cgactttcgc
      301 aacacggtca tcatcatgac ctcgaacatc ggcagcgagt ttctcgccag tcgttccggc
      361 gcgctgggct tcgtcgcgtc cgacacggcg ggcgggttcg gcagcgataa ggagctgcgc
      421 gatcgggtca tgggccggct tcgcgaggcg gtgcggcccg agtttttgaa ccgtatcgat
      481 gagatcgtgc tgttccgtgc cttggagaag ccgcagctcg aggagatcgt gcgcatgatg
      541 ctgcgcacga cggcggcgcg cctttcggag caagaggtca cgctcgatgt ttcggaggaa
      601 gcggtcggtt ggatcgccga acacggctac cagcgggagt acggcgccag gccgctgcgt
      661 cggctcatcc aacgcgaggt ggaagacagg gtggccgcgc tcctcatcga ggatgggctg
      721 cacgccggcc agcacgcggt cgtcgaggtt tcaggcgacg agcttcgtgt cgaggcccgc
      781 tgatcgggcc ggcgatcggt cgcggttgca agcgtcgggt actccttgtg gaaaaacgtc
      841 gattcttccg gcccagccac cccgtggcgg ggtgcacggc gtaatctcgc agatgcgggg
      901 cggtgggccg gagggctccg gccggcctcg gttgctgcgg tcagggtgag agggcatcgg
      961 tgagacgttg gtaagcggcg tggtcgggcc ggttctcgct cgactggcac tggggctggc
     1021 ccatgctcag gcgggttccg gcacgtcggg cagcggcgac acgaccgggt tcggcggcat
     1081 gaccggcatg gccggcatgg gcgggcccga catgcacggc gacatgtggc acccgacgct
     1141 gtatccgacg accgcgcgta tcgtggccat ccacctgcag cccattccga tcttgccggc
     1201 gctgtcggtc gccgcgttac tggcctacct cgcaggggtg tggatcttgc gccgtcgcgg
     1261 cacgcactgg ccggtgttgc gcaccttgtg gtgggtcatc ggcatcgcca cggttctggc
     1321 gatgacggcg accgcggtgg acggctacgg catggagctc ttcagtgtgc acatggtgca
     1381 gcatatggtg ctgaccatgc tgacgccgat cttcttgacg ctcggtgcgc cgctgacgat
     1441 gctcattcgt gtgctgcctc cgggcagcgg cccgttcaac gcgcgtcggc tgctactcct
     1501 tctgctgcac agccggttcg cacagttcgt cacccatccg gttgtgaccc tcatcgtctt
     1561 cttgatgagc ctttacgggc tctatttcac gccggtgttc gactggttga tgagctctat
     1621 gtggggccac aacctgatgc tcgcccattt tgtcttgatc ggcatgctct acttctgggg
     1681 catcatgggc gtcgatcctt cgcctcgccg ctacaacaac cagctacgtc gattcaacgc
     1741 ggaggtcgtg cggatcttcg agatcttcgt caccgtgccg ttccatgcct tcttcgggat
     1801 cgtcgtcatc atgtcgacga gcctcatcgt cggcttctac gccaacccga tgaaggggtg
     1861 gggcatttcg ccgctctccg atcaacgcac cgcaggcggt atcgcctggg ggttcacaga
     1921 gatcccgacg gttgtcttgc tgagcgctct cgtcatgatc tggcagcgtt ctgagaagcg
     1981 acacgagaag gccatcgacc accaggcgga actcaaccac gacgcggatc tcgtcgccta
     2041 caacgcatac cttgccaagg tctccgggca agacgccgcc acccctccga ggcgttgacc
     2101 ggctcctgcg acgcctgatc agtcgggcca gatgcccgat gtcccgcggc ggtggctgcc
     2161 gacgaggtgc gtgtcgatga cgccgatcgc ctccatcatg gcgaacatcg tcgtcggccc
     2221 gacgaacacg aagccgtggc cacgcaggtc gcgcgccaac gcctccgact cggccgagcg
     2281 ggtcggcacg tctgccaggc gccgtgggtg tggcgtcgtg gcgggccggt gtgcccagat
     2341 gagcttcgca aggccgcctt cgtcgcgcaa cgcgaccgtg gcccgggcgt tcgtgatcgc
     2401 ggcgccgatt ttacggcgat tgcggatgat ggccgcgtcg gccatgaggc gctccacgtc
     2461 gggctcgccg aacgcggcga cggcgtcggc atcgaagcct tggaaggcga gccgcagcgc
     2521 ggcgcgtttg cgcagaatgg tcagccacga caggcctgcc tgaaagccct ccaagatgat
     2581 ccgttcgaga agaccggcct cgtcggtgac gggcatgccc cattcggtgt cgtagtagcc
     2641 ctgcagcagg gggtctcgta tcgcccaggc ggggcgggcc ttaccatcgt cgcccgtcac
     2701 caggtcggaa gatatgcctg catcgacgcc ggctgtgaga cgtgccatgt cagtcgaccg
     2761 ccagaaggat cttgcccatg tgggcagagg attccatgcg tcggtgtgcg tcggcggccg
     2821 cagccagcgg gtacacggag tcgacgacga cgcgcaccct gccgtcgtcg accatcggcc
     2881 acacttcggc ttgcagcccg gccaggagtg cggctcgctc gtcgagcggg cggggccgca
     2941 ccgccgtgcc ctgcaggcga gcccgcttgg cgatgaggag accggcgggg aaagaggcgg
     3001 gcgtgccggt gcggtcggcg atcagcatga tgtgaccgcc ggtggcaaga acggcgaggt
     3061 cgcgagcgat gtagtcgccg ccgaccatgt caagcaccac gtcgactccg gcacccgccg
     3121 tcaatcggcc cacctcggag acgaagtcgg cctgtcggta gttcacggca tccgccccga
     3181 accttcggca gaaggccgcc ttctcctctg agccgaccgt cgccacgacg cgagcgccgt
     3241 gcgctcgcgc gatttggatc gccgcgagac ccacgccgct ggagccaccg tgcacgagca
     3301 agctctgccc tgccttcagg ccggcgaaga gacggatatt ggaccacgcg gtcacaagcg
     3361 cctcgggcag cgcggcggcg tccctcagac tcacgccgtg cggcacgggg agtacgagcg
     3421 actcgtgcac gctcacatac tcggcgtagc cgccgccgga taagagggcg cagacctcgt
     3481 cgccgatatc ggcccgggag gtattggcgc ccaccgccac gacccggccg gagacctcca
     3541 gaccgagcca ttccggtgca cccggcggtg gcgggtagtg cccgcggcgt tgcatgagat
     3601 ccgctccgtt gaccccggcg gccgctacct tgatgagaac ctcgcgctcg ccggggcggg
     3661 gtcgcggcac agttgtcaga gtcagtgcct ccggcccgcc gggggcggcg atcgtcacgg
     3721 catgcatcag gttgtcgtcg gtcacggttt ccaccctagg gcgattgcgg ccgacggcga
     3781 ccgctccggc cgactgctgc agaacctagc gcgacgacgg cgttgtgcag gagccggccg
     3841 gcccgtcgcg gggcggcgag gccccacgtc gagggcctcg gggcgtcgcg cccagccctc
     3901 tcaggcacag caggacagtt tcgagacgtc ggtggtaaag gccttcgcgg cgatccggat
     3961 gccttcggcc atcgtcaggt acggtaccca ggcgtcggcg acctcggtga tgctcttgcc
     4021 gagaaggtgg accccttctg ccgcgagctc gcccgcgccc tcagagaccg cggtcaggcc
     4081 gacgatctcg ccggtgtcga cgttcgcgac gatcttgatg aacccccgaa tgtcgcggtt
     4141 gaccagcgcc ctcggcacat ggctcaacgg caagacactg catgtgcaac ggatcccggc
     4201 agcgactgtc tccttctccg tcagcccgac cgtgccgacg gccgggctcg tgaaaatcac
     4261 ccggggcaag ccggtgaagg ccatgtgctc gtcggcgccg gcgagggcgt tgcctgcggc
     4321 gacggcaccc tcgcgggccg cgacgtagac gaactgcggg tgccccgtca cgtcaccggc
     4381 agcccagatc cgcggattcg aggaccgtag ccggtcggtg accacgactt cgccggcctc
     4441 gccggtctcg acgcctacgg tgtcgagggc gaggccgcac gtcgcgggcc gtcggccgag
     4501 cgcgacgagc acgcggtcgg tggggaactc ttgcgtgcgg ccagcgacct ccgcggtcac
     4561 gacggcctgg ccgtcgggtg cctcgcgggc cacccggagg gctaccgcgc ggcggaggac
     4621 acggatgtcc tcgtcggcga agaccttctg cagcgtctgt gcgacctcag gctcctccct
     4681 cgacgccagg cgggagcgcg ccaagagggt cactttcgag ccgagtcgtg cgaagagttg
     4741 cgcctgctcc agggcgacat agccaccgcc caggacaaga agggactccg gcagctcggt
     4801 gagttccatc gccgtggtcg aggtaaggta gccggccttc tcgaggcctt cgatcggcgg
     4861 aatccaggca cgagagccgg tcgcgacgag gtaggcgtcg gcttcgatcg tggccacgtt
     4921 cccctcgcgc gatgcgacac ggagggtcgg tacgtccggg gtgccggcga aggcagcgtc
     4981 gcccggcacc atgtcccagc cgtagaaatc ggcgatgtcg acgtacttct cggcgcgttg
     5041 cgcgtcgacg aggtcttgtt tgccgtcgat cagggccggc atgtcggcgg ggctcgcaga
     5101 ggtggttatc ccggggaatc gggccgtggc ttctgcggcg gtgtggcggg cttcagcggc
     5161 cgcgatcaga gctttggagg gcacgcagcc ggtgttcaca cagctgccgc cgatcgtgcc
     5221 gcgttcgacc atcacaaccg acttgcctag cccgaccgcc cgggccgcag ccgcgaaggc
     5281 ggcggcaccg gacccgatga tcgcgagatc gtacgtgttc tgcatgctgg ctccccttgg
     5341 gccgtgacga aaccggcccg acgtgaccat cgtcaacctt caagtacagt tgaaagtcaa
     5401 ggggcaatcg gcacgttcgg ggggtgagat gcggataggc gagcttgcgg atgcgggcgg
     5461 cacgacgacg gcgaccctcc gcttctacga ggagcaaggc ctcctgccgt ctccgggccg
     5521 tacgccgggc gggtatcgag actatgcgcc cgacgccgtg acgcgcgtcg acttcatcca
     5581 ccggggccag gccgccggcc tcaccctggc ccagatcggc cagatcctcg acatccgcga
     5641 ccaaggcctg gcgccgtgca ggcatgtgcg tgatcttctc gacacgcggc tggcagagat
     5701 cgatgcgcag ctcgcccggc tcgccgggct gcgagagacg atcgcagcat tgcgcgacgg
     5761 ggccgatcac ctggagcctg gcacctgcag cccagaccgg gtctgccgat acctctagcc
     5821 gtcgttcctc cgtgggttgt gaacgttcga ggtgggcggg gaccttcacg gtaggggcct
     5881 gccatgtatc ggctgccgcc ttgtctatag tgcggcgagg accgcgctgt gcaggaggcc
     5941 gttcgtcgcc agcgcgctgc cgtggctcgg cccctccgtg ccgtcgaggg cggtgaaccg
     6001 gccgcctgcc tcctccacga tcgggatgag tgctgccagg tcgtaggttt tcacgtcgta
     6061 ttcgccgaca atgtcgagca atccctcggc gagcatcatg tatgcccaca tgtctccgta
     6121 ag
//
LOCUS       DASTNL010000012         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_567754, whole genome shotgun sequence.
ACCESSION   DASTNL010000012 DASTNL010000000
VERSION     DASTNL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567754"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFQ96_00405"
     CDS             complement(<1..693)
                     /locus_tag="VFQ96_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256280.1"
                     /translation="MSTAEGMPLAATPDGAGTPRTGDGRRRGRLGWAFLFSDIGTLYR
                     RPRTWVLHAALAAIPILIAIAVKVTSAGPPPGRGPQFLDQITHNGLFVALTALLASAP
                     VFLPMTIAVVAGDTIAGEASHGTLRYLLLAPAGRFRLLVVKYLTCLVFCVSAPLAVAC
                     SGIGIGAILFPPGPVTLLSGDTVSFGDGVLRTGLIVLYAAVSLAGLCAIGLFVSTLTT
                     VPVGAMAAIIVAA"
     gene            complement(680..1633)
                     /locus_tag="VFQ96_00410"
     CDS             complement(680..1633)
                     /locus_tag="VFQ96_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0256281.1"
                     /translation="MSRGGARAIQTEGLTKRFGRHVAVDGVGLDVPHGSVFGFLGPNG
                     AGKTTTIRMLLGLVGAAGGRIELLGRRMPDEAATVLPRVGAMVEGPGFSPYLSGTANL
                     LRMDSADPRANWRDRKTRAAKALEKVGLGHAAQKKVHNYSLGMRSRLGLALTLLRPHD
                     LLVLDEPTNGLDPQGTREVRGIITGLAASGTTVFLSSHLLSEVEQMCSHAAVLRSGRL
                     VAQGSLSELGARSRVVDVETRDPNIVASLLASLGLHPQAAGGTAGTHVTADLPQESLE
                     PEEMLTRLVQAGARIGGFAVRGSSLEERYVALTGEGFDVDG"
     gene            complement(1630..2739)
                     /locus_tag="VFQ96_00415"
     CDS             complement(1630..2739)
                     /locus_tag="VFQ96_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256282.1"
                     /translation="MRRKLVRWIPAIAAPALVAAAVGLASIPAQADIPVKTPAQVVAL
                     AAGSKVSAFSGTLSQTSDLGLPSLPAVPESAAGPSSGDAATQLSALTGPHTARLYVAG
                     PQRQRLQVMSGGNEQDFVHDGTTAWLYDSATLTATRVTAPAGARDPAGQTPAPGNAMT
                     PQQLADRVLAAITPSTRVSVAGTTRVAGRDAYRLELQPKTSATLVDLVSIDVDAATGM
                     ALGVDITARGQATPALAVTFTSVSLRTPPASRFDFTPPADATLRHVTLPGEQGSARQG
                     QAPTIVGSGWTSVVELPGGTAADLLSGAAEAPTGTQGDAGAEQQAVLNGLTRAVAGGR
                     LLSTSLVNVYLTDTGRVFAGAVPPESLYAAARQAQ"
     gene            complement(2837..>3626)
                     /locus_tag="VFQ96_00420"
     CDS             complement(2837..>3626)
                     /locus_tag="VFQ96_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU0256283.1"
                     /translation="GAGGDPARVKGMVAYTIFATAIIFAIATISNDNLQDLKTGQLVR
                     ATPWKQQVALIIGVVFGSVLIAPILDLLNKAFGFVGAPGAGPDALPAPQAGLISALAQ
                     GVFGGKLNWGLVGLGVVIGAAVIVVDEILAKTTAKKRIPPLAVGMGIYLPMSLTLTIA
                     IGAVLGTIHDRWAARRPDPKRARRLGILMATGLIVGDSLWGVAYAGIVVASRTPEPMS
                     LVGASFAPWGDLAGIIVFVAVIVAMYRMARRLAGRPNEGAAERR"
BASE COUNT          537 a         1233 c         1359 g          497 t
ORIGIN      
        1 cgcggcgacg atgattgccg ccatcgcgcc gaccggaacg gtggtcagtg tcgacacgaa
       61 gagccctatc gcgcagagcc ccgccaggct gaccgccgcg tacagcacga tgagcccggt
      121 gcgcaagacg ccgtcgccga aactgaccgt gtcgccggag aggagcgtga ccggaccggg
      181 cgggaagagg atggcgccga tgccgatccc cgagcaggcg acggcgagcg gcgcgctcac
      241 gcagaacacc aggcaggtga ggtatttgac gacgagcagg cggaacctgc cggcgggcgc
      301 gaggagaaga tagcgcagcg tgccgtgcga cgcctcgccg gcgatcgtgt cgccggcgac
      361 cacggcgatc gtcatcggca agaagacggg ggcagaggcc agcagcgcgg tgagggcaac
      421 gaaaagcccg ttatgcgtta tctggtcgag gaactgcggg ccgcggccgg ggggcgggcc
      481 ggcggaggtg actttgacgg cgatcgcgat gaggatcggg atcgccgcga gtgcggcgtg
      541 cagcacccag gtgcgcggcc ggcggtacag ggtgccgatg tcggagaaga ggaacgccca
      601 gcccagcctg ccccgccggc gcccgtcgcc cgtccggggc gtgcccgcgc cgtcgggcgt
      661 cgccgcgagg ggcatgccct cagccgtcga catcgaaacc ttccccggtc aaggcgacgt
      721 agcgctcttc gaggctcgag ccgcgcacgg cgaagccgcc gatccgggcc ccggcctgca
      781 cgagccgggt gagcatctcc tccggttcga gggattcttg cggcaggtcc gcggtgacgt
      841 gggtgccggc cgtgcctccg gcggcctgtg ggtgcagccc gagggaagcc agcagcgagg
      901 cgacgatgtt cgggtcgcgg gtctcaacgt cgacgacgcg gcttctcgcg ccgagctcgc
      961 tgaggctgcc ctgcgcgacg agtcgcccgg agcgcagcac agcggcgtgg ctgcacatct
     1021 gttccacctc ggagagcaga tggctggaga ggaacaccgt cgtcccggac gcggcgagcc
     1081 cggtgatgat gcctcgcacc tcgcgggtgc cctgcgggtc gagcccgttc gtcggctcgt
     1141 cgagcaccag aaggtcgtga ggccgcagca gcgtgagggc gagcccgagc cggctgcgca
     1201 tgcccagcga atagttgtgc accttcttct gtgccgcgtg cccgaggccg accttttcga
     1261 gcgccttcgc ggcgcgcgtt ttgcggtcgc gccagttcgc ccgagggtct gcggaatcca
     1321 tgcgcaacag gttcgcggtg ccggaaagat acggcgagaa gcccgggcct tcgaccatgg
     1381 cgccgacgcg cggcagcacc gtggccgcct cgtcgggcat ccgccggccg agcagctcga
     1441 tacgcccccc ggcggccccc acgaggccga ggagcatccg aatggtcgtc gtcttgccgg
     1501 ccccgttcgg gccgaggaag ccgaagacgc tgccgtgcgg cacgtcaagg cccacgccgt
     1561 cgacggcgac gtgccggccg aacctcttgg tcagcccttc cgtctggatg gcacgcgcgc
     1621 cgcctctgct cactgcgcct gccgggccgc cgcgtacaag gattccggcg gcacggcccc
     1681 ggcgaagacc cggccggtgt cggtgaggta gacgttcacg aggctcgtgg aaaggagtcg
     1741 accgccggca acggcccggg tgagcccgtt cagcacggcc tgctgctcag ccccggcgtc
     1801 gccttgggtg ccggtcggag cctcggccgc gcccgacaac aggtcggcgg cggtgccgcc
     1861 gggcaattca acgacgctcg tccacccgga tccgacgatc gtcggtgcct ggccttgccg
     1921 tgcagaaccc tgctcccccg gcagcgtgac gtgccgcagg gtggcgtcgg ccggcggtgt
     1981 gaagtcgaag cggctcgccg gcggggtgcg aaggctcacg ctggtgaagg tcacggcgag
     2041 cgcgggcgtc gcttgaccgc gggctgtgat gtcgacgccc aacgccatgc cggtcgcggc
     2101 gtccacgtcg atactcacga ggtcgacgag ggtcgcggag gtcttcggct gcaactcgag
     2161 ccggtaggcg tcgcggccgg cgacgcgcgt cgtgcccgcc acgctcaccc gggttgacgg
     2221 cgtgatcgcc gcgagcaccc ggtcggcgag ctgctgtggg gtcatggcgt tgcccggggc
     2281 cggtgtctga cccgcagggt cacgcgcccc ggccggggcc gtgacacggg tcgccgtcag
     2341 cgtcgcggag tcgtacagcc acgccgtcgt gccgtcgtgc acgaagtctt gctcgttgcc
     2401 gccgctcatc acctggaggc gttggcgctg ggggcccgcg acgtagaggc gcgccgtgtg
     2461 cggcccggtc agcgcggaga gttgcgtggc ggcgtcgccg gaagacgggc cggcggcact
     2521 ctcgggcacg gccggcaggc tgggcaggcc gaggtcggag gtctggctga gcgttcccga
     2581 gaaggcggag accttgctgc cggctgccag ggcgaccact tgcgcgggag ttttcacggg
     2641 gatgtcggcc tgcgccggga tgctcgccag ccccacggcc gccgcgacga gggcgggagc
     2701 cgcgatggcc gggatccaac gaacgagctt gcgcctcacg acggtctccc ctcacgcttc
     2761 acaaacgagg ctacgccccg cttcgtgaac cttgccccag agaatgtgtc gcagggcccg
     2821 gccggagcct ctctgctcag cgacgctcgg ctgcgccttc gttcggtcgg ccggcgaggc
     2881 gccgggccat ccgatacatc gcgacgatga cggcgacgaa gacgatgatg cccgcgaggt
     2941 cgccccacgg cgcgaaagac gcaccgacca gcgacatcgg ctccggcgtg cgcgaggcga
     3001 cgacgatgcc ggcgtaggcg acgccccaca gactgtcgcc gacgatcagc ccggtggcca
     3061 tcaggatgcc gagccttcgt gcgcgcttcg ggtcgggccg gcgggctgcc caccggtcgt
     3121 ggatcgtgcc gagcacggcg ccgatcgcga tcgtcagggt gagcgacatg ggcaggtaga
     3181 tgcccatgcc gacggcgagg ggcgggatgc gcttcttcgc cgtggtcttc gcgaggatct
     3241 cgtcgacgac gatcacggcg gcgccgatca cgactcccag gccgacgagc ccccagttga
     3301 gcttgccgcc gaagacgcct tgcgccagcg cactgatgag gccggcctgc ggggcgggca
     3361 gcgcgtccgg accggcgccc ggggccccga cgaacccgaa ggccttgttc aagaggtcaa
     3421 ggatcggggc aatcaagaca gagccgaaga cgacgccgat gatgagggcc acctgctgct
     3481 tccacggcgt ggcacgcacc agctggccgg tcttcaggtc ttgtaggttg tcgttggaga
     3541 tcgtggcgat ggcgaagatg attgcggtcg cgaagatcgt gtaggcgacc atccccttca
     3601 cccgcgccgg gtcgccgccg gcgccg
//
LOCUS       DASTNL010000013         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_11689, whole genome shotgun sequence.
ACCESSION   DASTNL010000013 DASTNL010000000
VERSION     DASTNL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11689"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..124)
                     /locus_tag="VFQ96_00425"
     tRNA            complement(41..124)
                     /locus_tag="VFQ96_00425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Leu,seq:cag)
     gene            482..1561
                     /locus_tag="VFQ96_00430"
     CDS             482..1561
                     /locus_tag="VFQ96_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0256284.1"
                     /translation="MTHDSAALKVLILGGTGTISASCVRLAVASGMRVTVLNRGTPHA
                     GRELPPAVERLVGDVADEKTLREAARGRDLDAVVDFLSYDEHTAARAVRAFAGTTGQY
                     VHISSASIYTKPLRQIPLTESSPVGPNPALPYATAKWRAEQTLVKAHDEEGFPVTIVR
                     PSHTYDDASPVLPGGWSIIDRIARGAEVPVPGDGTSLWTLTHAEDFAQGLIGLVGNTR
                     ARGEIFTITGSDVYTWDQIYTIVAEALGVAPQLVHVPAEFYPLVAPDWFWSGEVAGDL
                     GHSAVFDGTKIRRFVPGFAPRLTFGRAIHRILAWRSAHPDEAAPEPTTDAVIERIVGA
                     YHAAREVFLASAGTTAGHTEEHAAS"
     gene            1558..2604
                     /locus_tag="VFQ96_00435"
     CDS             1558..2604
                     /locus_tag="VFQ96_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0256285.1"
                     /translation="MSNTEVKPEEVLCRDGGLPGPRSAQASGVPVEIIASREVPLGGP
                     RAMLVRRTLPQRQRSMIGAWCFVDHYGPEDVSVTGGMDVAPHPHTGLQTVSWLFAGAI
                     AHLDSGGNSGTVLPGEMNLMTAGAGICHSEVSTGATTILHGAQLWLALPDRVRKSAGR
                     RFEHYAPPAQRFEGGEALVFLGTLTGSSSPVAAYSPVVGAEIRLAPAAEAALDVDPAF
                     EHGVLVDQGEVSLEGVPVPAGAIGYTGVGSSVLHLRNDAPSPARMLLIGGAPFDEEIV
                     MWWNFVGRDSDEIARYREEWQAESDLFGRVEGYVGHGGPGAGGAGMPRLPAPVLPALR
                     LRPRRNRPPHVQGV"
     gene            2718..>3156
                     /locus_tag="VFQ96_00440"
     CDS             2718..>3156
                     /locus_tag="VFQ96_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HEU0256286.1"
                     /translation="MGILDNVERGLERAVNGAFAKTFRAKLQPVELASALRRELDTHA
                     AVLDRERTVAPNSFHIRVSTADLERLRGLGETLTDELVAAVSKHAARQGYQLAGPAEV
                     DLVEDRALGEGMLAIDSETVRRTIVWAPALDIAGQRRPLTGHRT"
BASE COUNT          483 a         1147 c         1087 g          439 t
ORIGIN      
        1 cagagagtta aatcccaggt cagaggccat ttctgactcg tgcgcgaggg gggacttgaa
       61 cccccacgcc cttgcgagca caggcacctg aagcctgcgc gtctaccgat tccgccactc
      121 gcgcgaacct cagcagacta acatcccccg cggccggcgc gctatccgcc agccccgcac
      181 gccctccggc acgcggctca tccgcccggg ccccgcgcag ggacggcggt ggcccgcctc
      241 tggcgcaaga acgctcgccg actataaggt gtgaaccagc cggagcatcg gcgacgcgga
      301 ggaccgcggg aggcagcgcg gcgtcacacg cgtgggtgcg agggggcagc atggcgtcgc
      361 gcgacggggc gactgtggtg cacaccgacg gcagtgaggc gcaggcagac gcacagtggc
      421 aaccccccga cagggaatgc gggccggccc ggcgcgggca ggaagacggg caacgatggt
      481 gatgactcac gacagtgcgg cactcaaagt gctcatcctc gggggtaccg gcacgatcag
      541 tgcgtcgtgc gtgcgcctcg cggtggcctc gggaatgcgg gtcacggtgc tgaatcgcgg
      601 caccccgcat gccggccggg agctgccgcc ggccgtcgag cggctcgtcg gcgacgtcgc
      661 cgacgagaag acgttgcgcg aggcggcacg aggacgcgac ctcgacgccg tcgtcgactt
      721 tctctcctac gatgagcaca ccgcggcccg ggcggtgcgt gccttcgccg gcacgaccgg
      781 ccagtacgtg cacatcagct ccgcctcgat ctacaccaag cccctccggc agatccctct
      841 caccgaatcc agcccggtcg gcccgaatcc cgcgctgccg tatgcgaccg cgaaatggcg
      901 tgccgagcag accctcgtga aagcgcacga cgaagagggc tttccggtga cgatcgtgcg
      961 cccctcccac acctacgacg acgcctcccc cgtgctgccc ggcggctgga gcatcatcga
     1021 tcgcatcgct cgcggcgccg aggttcccgt gcccggcgac ggcacgtcgc tgtggaccct
     1081 cacccacgcg gaagacttcg cacaggggct catcgggctc gtcggcaaca cgcgggcccg
     1141 gggcgagatc ttcaccatca cgggatccga cgtctacacc tgggatcaga tctacacgat
     1201 cgtggcggag gcgctcggcg tcgccccgca gcttgtgcac gtgcccgccg agttctaccc
     1261 gctcgtcgcg cccgactggt tctggtcagg ggaggtcgcg ggagaccttg gccactcggc
     1321 ggtcttcgac ggcacgaaaa tccgtcgctt cgtgcctggc ttcgcaccgc ggctcacgtt
     1381 cggccgcgcc atccaccgca tcctcgcctg gcggtccgca caccccgacg aggcggcgcc
     1441 cgagcccacg accgacgccg tcatcgaacg catcgtgggc gcctaccacg ccgcccgaga
     1501 agtcttcctg gcgagcgcgg gcacgacggc aggccacaca gaggagcacg ccgcgtcatg
     1561 agcaacacgg aggtcaagcc cgaagaggtg ctctgccgcg acggcggcct gccagggcct
     1621 cgctccgcac aggcgagcgg cgtgccggtc gagatcatcg cctcccgcga ggtgccgctc
     1681 ggcgggccgc gggccatgct ggtgcggcgc accctgccgc agcggcaacg ctccatgatc
     1741 ggcgcgtggt gtttcgtcga tcattacggc ccggaggatg tctcggtcac cggcggcatg
     1801 gatgtcgcac cgcatccgca cacgggcctg cagacggtga gttggctgtt cgccggcgcg
     1861 atcgcgcacc tcgactcggg cggtaattcc ggcaccgtgc tgcccggcga gatgaacctc
     1921 atgaccgcgg gcgccggcat ctgccacagc gaggtgtcga ccggggcgac gacgatcctg
     1981 cacggcgcac agctctggct ggccctgccc gaccgggtgc gaaagagcgc cgggcgacgg
     2041 ttcgagcatt acgccccgcc ggcacaacgg ttcgagggcg gtgaggccct cgtcttcttg
     2101 ggcaccctga ccggctcctc ctcccccgtg gcggcgtact caccggtcgt cggcgcggag
     2161 atccggctcg caccggccgc cgaggccgcc ctcgacgtcg acccggcttt cgaacacggc
     2221 gttctggtcg accagggcga ggtgtcgctg gagggtgtgc ccgttcccgc cggcgccatc
     2281 ggctacaccg gcgtcggcag cagtgtgctg caccttcgca acgacgcccc gtcgccggct
     2341 cggatgctcc tcatcggggg cgcaccgttc gacgaagaga tcgtcatgtg gtggaacttc
     2401 gtcggccgcg actccgacga gatcgcccgg taccgcgagg aatggcaggc cgagagcgac
     2461 ctgttcggcc gggtcgaagg ctacgtcggg cacggcggcc cgggtgcggg cggggcgggc
     2521 atgcctcgcc tgcctgcccc tgtgcttccg gcactgcggc tgcggccccg gcggaaccgc
     2581 ccgccgcacg tgcagggcgt gtaaccgacc acactttccc gaggcggccg ccgggcgggg
     2641 cggcccacag atgctcagtg tcgtcgcacc cgttcccgct accattcaca tagtcgcgcg
     2701 cggtaggtgg gagacgagtg ggcatcttgg acaacgtcga aagggggctg gagcgcgccg
     2761 tgaacggcgc cttcgccaag accttccgtg ccaagctgca gccggtcgag ttggcgagcg
     2821 ccctgcgccg cgaactcgac acccacgccg cggtgctcga ccgggagcgt accgtcgcgc
     2881 cgaacagctt tcacatccgc gtctccacgg ccgacctgga aaggctgcgc ggtctcggtg
     2941 agacgctcac cgacgagctg gtcgcggcgg tgagcaagca cgccgcacgg cagggttacc
     3001 aactggcggg ccccgcggag gttgacctcg tcgaagaccg cgcgctcggc gaggggatgc
     3061 tcgccatcga ctccgagacc gtgcgtcgca ccatcgtgtg ggcgccggcc ctcgacatcg
     3121 cggggcagcg tcgccccctc accggccacc gcacgg
//
LOCUS       DASTNL010000014         5444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1123926, whole genome shotgun sequence.
ACCESSION   DASTNL010000014 DASTNL010000000
VERSION     DASTNL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123926"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..441)
                     /locus_tag="VFQ96_00445"
     CDS             complement(142..441)
                     /locus_tag="VFQ96_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0256287.1"
                     /translation="MSSREVCDLLAERGMPLSLTTVITGLQRLTRKGVLLRHPGRSRR
                     TWLFRPAQSPAERIADLMGDALAGAIDREAALLQFAGNLDDEDLDVLRSALEDQK"
     gene            659..2320
                     /locus_tag="VFQ96_00450"
     CDS             659..2320
                     /locus_tag="VFQ96_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0256288.1"
                     /translation="MPFPSPHRLLLPAVATAFLLALTGCTAGEATPGPAASHSQAPER
                     GGTLVYASGDAEPTCLDPHVGGNYPQALVATQYLEELVSENSKHEIIPWLATSWQRAA
                     DGLSVTFTLRKGVRFTDGTPLDAQAVAANIAQIKDPKTGSSTGYLALGAVKSTTVLSA
                     RKIRFNLSRPDSALLESLSQPWLAIESAKAIARPQAVNCVHPVGTGPFKVASWVRQNS
                     ITLVRNAGYNSPPADARHRGPAYLSKILWRFIPDSATRYAALQSGEVQVIDNAQPDTI
                     QAAEKTKTLQQLNAPRPGASDRIELDSGKPPFDDKRVREAFIRAAQVNPGIKRLFFGT
                     ASRSYSALSSVEPLAYSDKKLFRYDPAEANRLLDEAGWNKRDKAGYRTKDGKRLTVVF
                     PVSTNQSIPAEQSLFEQIQASTKEVGFDVKLEPMDLGDWYAALAANQYNAVSAPYTKA
                     GPDVLRILYDSAGIKPAPSGYFANLAQVDIPSLDKALQKAAEVSNPTERAALYTKAQK
                     TVLSGYYLLPLYDQQNHYLLRDDVVGLRALPSVSTPVFYDTWLRR"
     gene            2407..3396
                     /locus_tag="VFQ96_00455"
     CDS             2407..3396
                     /locus_tag="VFQ96_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256289.1"
                     /translation="MRRRPRPSRARAGRRLVGHLAARAAGFATVLWAVATLTFFAIRL
                     IPGDPALAILGGPGSEAGPAALAHVQAQYGLDKPLLVQYLDQLGRYLTGNLGTSYSLH
                     EPVAQVIGSQLGGTLTLAVASLAVGWAIAVTAASAAVWGGRVGNACASGLEIAAAAMP
                     HFWLGTVLILAFSTALGWLPAVSVPGPVGLILPVTTLAVPLAGFLGQIMRESLTAAAA
                     APFALSARARGESELGVLLRHTLRHAAVPAVALTGWAFGSLISGAVVVETIFARPGLG
                     RTLLGAVQQRDVPLVTGVVLAVALAYCIATVAGEAGERLLDRRSRPGGPEGGR"
     gene            3440..4222
                     /locus_tag="VFQ96_00460"
     CDS             3440..4222
                     /locus_tag="VFQ96_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256290.1"
                     /translation="MILSSAVLLWLLACAFFPHLLAPADPLAVHPARGFLPPSPAAIL
                     GTDESGRGVYTRIVHGAAASLSIGAGATALGIVLGTLLGTMAGLGPRWLDFGTSRLLE
                     VLFAFPGLLLALLFIVVYGPGGATSAIAVGLATAPGYARIIRSQMITVRQAPYVEALV
                     VLGRGPWHRFWHSILPNVAEALFALVTLGVGQSIVWASSLSFLGLGTPPPAAEWGAML
                     ADGRTYLGTAWWMTFFPGLVIVLAAAACTVLGRRLQGKARRS"
     gene            4219..>5444
                     /locus_tag="VFQ96_00465"
     CDS             4219..>5444
                     /locus_tag="VFQ96_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256291.1"
                     /translation="MSTRRRAEPANEHALPHQAGPARSAAPGDPGAGVPDDPGAGVPG
                     DPGAAGAAPAGERAPAAAPVLSVTGLRVGFPAPGTRRVTPAVRGIDLTVRPGECLALV
                     GQSGSGKSVTARALLGLVGGHAVVEAARLRLGETDLLALSRRAWRRVRGARIGMVLQD
                     ALTSLDPLRPVGREIDDALRLHTRLGPAGRRARMLQALADAGIADPELRAAGRSDELS
                     GGQRQRALIAAAIVLNPALIIADEPTTALDVAVESQVLDTFGGLRDRGTALLLISHDL
                     SVVARVADRVAVMHDGLIVEEGTAAEVLRRPRHPYTVELVGAVPEGRPRGTLLSLAGR
                     GSATNAAPRPGVRPSPVTPHAADALRACVAPAAGGHPHAAPRQAPASRDVAVAALELR
                     GVSRAFHTPEGTRPAV"
BASE COUNT          766 a         2086 c         1811 g          781 t
ORIGIN      
        1 cgaagacgac cgcggcctgc caggccagca tcgcggccca cggatgccac acccgccagg
       61 agcccgtcac gagcagccac ggcagcacca ggcaggtcaa cgacgccgca cacagccagg
      121 tgacggccag cgcggccatg ctcatttctg gtcttccagc gcgctgcgca gaacgtcgag
      181 atcctcgtcg tcgaggtttc cggcgaactg caagagtgcc gcctcacggt cgatcgcgcc
      241 cgccaaggcg tcacccatca agtcggcgat gcgctccgcg gggctctgcg ccggccggaa
      301 caaccacgtg cggcgtgagc gcccaggatg gcgcaaaagc acacctttgc gggtcagccg
      361 ttgcagcccc gtgatcaccg tcgtcagcga cagcggcatg ccacgttcgg ccaagagatc
      421 gcagacctcg cggctcgaca tcgcggccgg agaagacgac agcgtctcca cgatcgcccg
      481 ttcgagcgac cccctggccc tgcgtgtcga acccaccctc gaacgttacc gcacgggcgc
      541 gccgggcccc gccggattga ccgggagcat cactatcctt attgttggtc tcagaccaac
      601 aatattctct cctgcggggc cgagcccgac tcgtcaccgc atccgacagc gaggcatcat
      661 gccgttcccc tccccccacc gcctgctcct gcccgccgtc gccacggcgt tcctcctcgc
      721 cctgaccgga tgcaccgcgg gcgaagccac gcccgggcct gccgcctcgc actcccaggc
      781 accagagcgc ggcggaaccc tcgtctacgc cagcggcgac gccgagccga cctgtctcga
      841 cccgcatgtc ggcggcaatt acccgcaggc actcgtcgcg acgcagtatc tcgaagaact
      901 cgtctccgag aacagcaagc acgagatcat cccgtggctg gccacctcgt ggcagcgggc
      961 cgccgacggg ctcagcgtga ccttcaccct gcgaaagggc gtgcggttca ccgacggcac
     1021 gccgctcgac gcccaggccg tcgcggcgaa catcgcgcag atcaaagatc cgaagaccgg
     1081 ctcctccacc ggatacctgg cgcttggcgc cgtgaagagc acgaccgttc ttagcgcgcg
     1141 aaagatccgg ttcaacctca gccgccccga cagcgcgctc ctcgaatccc tctcccaacc
     1201 gtggctcgcc atcgaatccg ccaaagccat cgcccggccg caggccgtca actgtgtcca
     1261 cccggtcggc accggaccct tcaaggtcgc ctcgtgggtg cgccaaaact cgatcacgct
     1321 cgtgcgcaac gccggttaca actccccgcc ggccgacgcc cggcaccggg ggcctgcgta
     1381 cctgtcgaag attctctggc gcttcatccc cgactccgcg acccgatacg cggccctgca
     1441 gtccggcgag gtgcaggtga tcgacaatgc ccagcccgac acgatccagg ccgcagagaa
     1501 gacgaagacg ctgcagcagc tgaatgcgcc acgccccggc gcgagcgacc gcatcgagct
     1561 cgacagcggc aagccgccct tcgacgacaa gcgggtgcgc gaggcgttca ttcgggcggc
     1621 ccaggtgaac ccgggcatca agaggctctt cttcggcacg gcgagtcgtt cctattcggc
     1681 actctccagt gtcgagccgc tcgcctactc cgacaagaag ctgttccgat acgacccggc
     1741 cgaggcgaac aggctgctcg acgaggccgg ctggaacaag cgtgacaagg ccggctaccg
     1801 cacgaaagac ggcaagcgac tcaccgtcgt cttcccggtc tccacgaacc agtcgatccc
     1861 ggccgaacag tcactcttcg aacagatcca agcctcgacg aaagaggtcg gcttcgacgt
     1921 gaagctggag ccgatggacc ttggcgactg gtacgccgca ctcgccgcca accagtacaa
     1981 cgcggtgagc gccccttaca cgaaggccgg gcccgacgtg ctgcgcatcc tctacgactc
     2041 ggccggcatc aagcccgcgc cgagcggcta cttcgcgaac ctcgcccagg tcgacatccc
     2101 ctccctcgac aaggcgctgc agaaggcggc cgaggtctcg aacccgaccg aacgggccgc
     2161 cttgtacacg aaggcgcaga agacggtgct ctcgggctat tacctgctgc cgctctacga
     2221 ccagcagaac cactacctgc tgcgcgacga cgtcgtgggc cttcgggcac tgccgagcgt
     2281 ctcgacgccg gtgttctacg acacgtggct gcgacggtag gtggcgcccg gcctgccaca
     2341 ggccacctcg caccaggcca cccggcgacc cgggcgcagc ctcggcacga agagaccccg
     2401 ccagcgctga gacggcggcc tcgcccaagc cgcgcgcggg ccggccggcg cctcgtcgga
     2461 cacctcgcgg cccgggcagc gggattcgcc accgtgctct gggcggtggc gacgctgacg
     2521 ttcttcgcga tccggctgat tcccggggat ccggccctcg cgatcctcgg cggccccggg
     2581 tccgaggccg ggcccgccgc gctcgcgcac gtgcaggcgc aatacgggct cgacaagcct
     2641 ctgctggtgc aataccttga ccagctgggc cgctacctga ccggcaatct cggcacgtcg
     2701 tattcgctgc acgagccggt ggcgcaggta atcggctccc aactgggcgg caccttgacg
     2761 ctcgccgtcg catcgctcgc cgtcggatgg gcgatcgcgg tcaccgcggc gagcgcggcg
     2821 gtctggggtg ggcgcgtcgg gaatgcttgc gcctccgggc tggagatcgc ggcggccgcg
     2881 atgccacatt tctggctcgg caccgtgctg atcctcgcct tcagcacggc gcttggctgg
     2941 ctgcccgcgg tgagcgtgcc gggcccggtc ggcctgatcc tgccggtcac aaccctggcc
     3001 gtgccgctcg cgggctttct cggccagatc atgcgcgagt cgctgacagc cgccgcggcc
     3061 gcccccttcg ccctctccgc aagggcccgc ggcgagtccg agctcggggt gctcctgcgc
     3121 cacacactgc gccatgccgc cgtcccggcg gtggccctga ccggctgggc gttcgggtcc
     3181 ctcatcagcg gtgccgtcgt cgtcgagacg atctttgccc gaccgggcct cggccgcact
     3241 cttctgggcg ccgttcagca acgcgacgtg ccgctcgtca ccggcgtggt gctggccgtc
     3301 gcgctcgcct actgcatcgc cacggtcgca ggggaggccg gcgaacgcct cctcgaccgg
     3361 cgctcccggc cgggcgggcc cgagggaggg cggtaacccg atgcgacggt tctgggcgct
     3421 agcgccgcga caggcggccg tgatcctctc gtcggccgtg ttgctctggc tcttggcatg
     3481 cgccttcttt ccccacctct tggcgcccgc cgacccgctg gcggtgcacc cggcacgcgg
     3541 gttcctgccc ccctcgccgg ccgccatcct cggcaccgac gagtcgggcc gcggcgtcta
     3601 cacccgcatc gtgcacggtg ccgccgcctc gctctcgatc ggggccgggg cgactgcgct
     3661 cggcatcgtg ctcggcaccc tgctcggaac gatggcgggg ctcggcccac gctggctcga
     3721 tttcggcacg agccgccttc tcgaggtctt gttcgccttt cccggcctgc ttctcgccct
     3781 gctcttcatc gtcgtgtatg ggccaggcgg ggcgacctcg gccatcgcgg tgggtcttgc
     3841 gacggcaccg ggctacgcgc ggatcatccg ctcgcagatg atcaccgtgc gtcaggcccc
     3901 gtacgtggag gcactcgtcg tgctcggccg cgggccctgg caccggttct ggcacagcat
     3961 cctgccgaac gtcgccgagg cgctcttcgc gctcgtcacg ctcggggtcg ggcaatccat
     4021 cgtctgggcg tcgtcgctca gcttcttggg gctcggcacc ccgccaccgg ccgccgaatg
     4081 gggcgcgatg ctcgcagacg gtcgcaccta cctcggcacc gcgtggtgga tgacgttctt
     4141 tccgggcctc gtgatcgttc tggcggccgc cgcctgcacg gtgctaggcc ggcgcctgca
     4201 agggaaggca agacggtcat gagcaccagg cgacgcgcgg aacccgcaaa cgagcacgca
     4261 ctgccccacc aggcagggcc cgcccggtcg gcggcgcccg gcgatccggg ggcgggcgtg
     4321 cccgacgatc cgggggcggg cgtgcccggc gatccggggg ccgccggcgc cgcacccgcc
     4381 ggcgagcggg cgcccgccgc ggcacccgtg ctgagcgtca cggggcttcg tgtggggttc
     4441 cccgcgccgg gcacgcggcg cgtcacaccc gcggtgcgcg gcatcgacct tacggtgcgg
     4501 ccgggggaat gcctcgccct cgtcggccag tcgggctccg gcaaatcggt caccgcgcgc
     4561 gccctcctgg gcctcgtggg cgggcacgcc gtcgtcgagg ctgcgcggct gcgcctcggc
     4621 gagacagacc ttctggccct gtcgcgacgc gcgtggcggc gggtgcgcgg ggcgcgcatc
     4681 ggcatggtgc tccaagacgc gctgacctcg ctggacccgt tgcggccggt cggccgcgag
     4741 atcgacgatg cgctgcggct gcacacccgt ctcggcccgg ccggacgtcg cgcccggatg
     4801 ctccaggcgc tggccgacgc cggcatcgcc gaccccgagc tgcgggcggc ggggcgctct
     4861 gacgagctct ccggcgggca gcgacagcgg gctctgatcg ccgccgcgat cgtcctgaac
     4921 cccgcgctca tcatcgccga cgagccgacg acggcgctgg atgtggccgt cgagagtcag
     4981 gtgctcgaca cgttcggggg gcttcgcgac cggggcaccg cacttctcct catcagtcac
     5041 gacttgagcg tggtcgcgcg cgtcgccgac cgcgtcgcgg tcatgcacga cggcctcatc
     5101 gtcgaggaag gcaccgcagc cgaggtcttg cggcggcccc gacaccccta cacggtcgaa
     5161 ctcgtggggg cggtgcccga gggtcgcccg cgcggcaccc tcctgagcct cgcggggcga
     5221 gggtccgcca cgaacgccgc gccccggccg ggggtccggc cgagccccgt caccccgcac
     5281 gcggccgacg cgctgcgggc ctgcgtcgcc ccggctgccg gcggccaccc gcatgccgcg
     5341 ccccggcagg ctcccgcctc gcgcgacgtg gccgtcgccg cgctggaact gcgcggcgtc
     5401 agccgcgcct tccacacccc ggagggcacg cggccggccg tcga
//
LOCUS       DASTNL010000015         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_753841, whole genome shotgun sequence.
ACCESSION   DASTNL010000015 DASTNL010000000
VERSION     DASTNL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753841"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFQ96_00470"
     CDS             complement(<1..356)
                     /locus_tag="VFQ96_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256292.1"
                     /translation="MSTVTNGGAQHVALASRTRREILDMVTSSECPLDAATIAERMNL
                     HVTTVRFHLDRLEAAKLLSRTTEREGHRGRPRVVYSTDAFPSDENAANRQLVEVLADA
                     LSHDPDGGKARGEEAGR"
     gene            534..>2011
                     /locus_tag="VFQ96_00475"
     CDS             534..>2011
                     /locus_tag="VFQ96_00475"
                     /inference="COORDINATES: protein motif:HMM:NF011538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HEU0256293.1"
                     /translation="MLLAARGVFPDDAVTVVPCDDDGAAAGEPVALPGGALARYVARH
                     QDERWAWPDTATWYPALVERGVRVLRCHDLRLSRAILAASGYCRPGVFSAGPAWRQGQ
                     AHPAARDDAALFDWPGGEATLGLDEAGREARLQAEAIRTSAHPGRLRLLVAAESAGAL
                     AAVEMTWAGIPWRADVHDAMLRRVLGPRPRGRERPALVEKAAEHLRTVLGTTALNPDS
                     PAELLAALRRSGLRVDSTSAHTLEGVDTPATRALADYRRLARLWSANGWTWQDRWVQG
                     GRLHAEYVPGGVVTGRWAGHGGGALQLPKKVRPAVVADPGWKLVVADAAQLDPRILAA
                     LADDRAMIAAGAGSDLYRGIVERGVVPDRRRAKVAMLGAIYGATTGDSARLMPSLAAA
                     FPRAIGLVEAAAATGRAGGVVTTRLGRTAPHPGAAWRQLQRRAAEPGASAALAHAARR
                     QAGDRSRFIRNFVVQGTAAEWALCWLAGTRARLAGLAPRRDDL"
BASE COUNT          237 a          713 c          780 g          281 t
ORIGIN      
        1 cgcccggcct cttcgccccg ggccttgccg ccgtcggggt cgtgagacag tgcgtccgcg
       61 agcacctcga cgagctgccg gttcgcggcg ttctcgtcgg aggggaacgc gtcggtggaa
      121 tagacgacgc gcgggcggcc gcgatggcct tcacgctccg tcgtgcggga gagcagcttc
      181 gccgcctcga gccggtcgag atgaaagcga accgtcgtga cgtgcaggtt catgcgttcc
      241 gcgatagtcg cggcgtcgag ggggcattcc gacgacgtca ccatgtcgag aatctctcgc
      301 cttgtccgtg aggcgagagc cacgtgttgt gcgccgccgt tggtgaccgt actcattctt
      361 taagactagc gcgctccgtt ttatgttcgg catggcgggc cggccgcgtc gacgcgacgc
      421 gacgcgtacc tgcacccgga tggcctcgcg gcgcccgtcg ggcgacggac acggcggatg
      481 agggggccga ggaagagccc ctgcggcact gcgcccgagg cgagaatggc agtgtgcttc
      541 tggcggcccg cggcgtgttt cccgacgacg ccgtcaccgt cgtcccgtgc gacgacgacg
      601 gcgccgcggc cggggagccc gtcgccctgc cgggcggcgc cctggcgcga tacgtggcac
      661 ggcaccagga cgagcgttgg gcctggcccg acacagctac gtggtacccg gcgctcgtgg
      721 aacgcggcgt gcgggtcttg cgctgccacg acctgcggct ctcccgcgcg atcctcgcgg
      781 catccggata ctgccggccg ggggtcttct cggccggccc ggcgtggcgg cagggccagg
      841 cgcatccggc ggcccgcgac gacgccgcgc tgttcgactg gccgggcggc gaggcgacgc
      901 tcggcctgga tgaggcgggg cgagaggcgc ggttgcaggc cgaggcgata cgcacctccg
      961 cgcatccggg ccgcctccgg ctgctcgtcg ccgccgagtc ggcgggcgcg ctcgccgccg
     1021 tcgagatgac atgggcgggc atcccctggc gtgccgacgt tcacgacgcg atgctgcggc
     1081 gggttctggg cccccggccc cgcggccgag aacggccggc ccttgtggag aaggccgccg
     1141 agcacctgcg cacggtgctt ggcactaccg ctctgaaccc ggattcgccg gcggaactgc
     1201 tcgcggcgct gcgccgttcc gggctgcgcg tcgattcgac gagcgctcac accctcgagg
     1261 gcgtcgacac gcccgcgacc cgggcgttgg ccgattaccg gcgcctggcg cggctctggt
     1321 cagccaacgg ctggacgtgg caagaccggt gggtacaggg tggccgtctg cacgccgaat
     1381 acgtgccggg cggcgtcgtg acggggcggt gggcagggca cggcggcggg gcgctgcagc
     1441 tgcccaagaa agtgcgtccc gccgtcgtcg ccgacccggg gtggaagctc gtggtcgccg
     1501 atgcggctca gctcgacccg cgtatcctcg ccgcgctcgc cgacgaccgg gcgatgatcg
     1561 cggcgggtgc gggcagcgac ctctatcgag gcatcgtcga gcgtggcgtc gtgcccgacc
     1621 ggcgccgcgc caaggtcgcg atgctgggcg ccatctacgg cgcgaccacg ggggatagcg
     1681 cccggttgat gccgtcgctt gcggccgcgt tccctcgcgc gatcggcctc gtcgaggccg
     1741 cggcggcgac cgggcgggcc ggcggggtcg tcacgacgag gctcgggcgc accgcgccgc
     1801 acccgggtgc ggcctggcgg cagttgcagc gacgggcggc ggagcccggc gcgtcggcgg
     1861 cactggccca cgcggcccgc cgtcaggcgg gcgaccgcag caggttcatc cgcaacttcg
     1921 tcgtgcaggg caccgccgcc gaatgggcgc tgtgctggtt ggccggcacc cgcgccaggc
     1981 tggccgggct cgcgccgcgc cgcgacgacc t
//
LOCUS       DASTNL010000016         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1311189, whole genome shotgun sequence.
ACCESSION   DASTNL010000016 DASTNL010000000
VERSION     DASTNL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311189"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1603
                     /gene="purD"
                     /locus_tag="VFQ96_00480"
     CDS             338..1603
                     /gene="purD"
                     /locus_tag="VFQ96_00480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU0256294.1"
                     /translation="MKILVLGSGAREHAIVKALLGEDAGHEITAAPGNAGIAADVATV
                     PLDADSPEDVANYALEHDIELVVIGPEAPLVAGVADALRTRGIPVFGPGQAAAQLEGS
                     KSFAKRIMDAAGVPTGRSIRAASLLEAEEALDTFGAPYVVKADGLAAGKGVLVTHDRE
                     AALEHASTWLVHEAVLVEEFLDGPEVSLFLISDGHTVLPLAPAQDYKRLGDGDAGPNT
                     GGMGAYSPLPRLDERFVDEVIETIALPTVRQLAEEQTPFIGLLYCGLIQTPAGIRVIE
                     FNARFGDPETQVVLPRLVTPLSTLLFAAATGALAGLPRPEFALDVAVTVVLASEGYPS
                     APVTGRKITGLDAAAQVPEVQILHAATAVDPASGALLATGGRVLNVVAVGTTFDEARS
                     RAYEAMGRIRLEGGRYRSDIAAGVGDVTR"
     gene            complement(1636..>2032)
                     /locus_tag="VFQ96_00485"
     CDS             complement(1636..>2032)
                     /locus_tag="VFQ96_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEU0256295.1"
                     /translation="VCSGLLAFFPTDVATAGRSHAVAPVQGTVHGGTVHGSIHLALAG
                     IGFAAALAGVLVLTGWLRRGASAGRPGPAASAFAPTVLAAIAGVAFAAVVLTVRLRPG
                     VFGVSERVFIAAILAWTVVVALRLRRVTD"
BASE COUNT          311 a          753 c          704 g          264 t
ORIGIN      
        1 gcgcctccgc ctcgcggatc tgctgcgccg tgtatgcctt cgtcacggtt tcagggtgtc
       61 actccggggg tcgagtggcg agcttgcgag cgtatcgaga gcacccaccc accggcggtc
      121 gagtagcgac gaaggagcgt atcgagacca cgcacgcacg gccccggggt ctcgatacgg
      181 tcgctgcgcg acctgctcga ccaccgcagg ggcaccaggt ctcgatacgg tcgctgcacg
      241 acctactcga ccatcggagg ggcctactcg accaccgcag cgcagggggg tactcgacca
      301 ccgcagcgtc gacggcgcct ctggctaggc tgagaccgtg aaaattctcg tcctcggttc
      361 cggtgcccgc gagcacgcca tcgtcaaggc ccttctcggc gaggatgccg ggcacgagat
      421 caccgccgcc cccggcaacg cgggcatcgc cgccgacgtc gcgacggtgc cgctcgacgc
      481 cgactccccg gaggacgtcg caaattacgc gctcgagcac gacatcgagc tcgtcgtgat
      541 cggaccggag gcgccgctcg tcgccggcgt cgccgacgcg ttgcgcaccc gcggcatccc
      601 ggtcttcggc cccgggcagg ccgccgcaca gctggagggg tccaagtcgt tcgcgaagcg
      661 catcatggac gccgccggcg tgccgaccgg acgctcgatc cgggcggcca gtcttctgga
      721 ggcggaggag gcgctcgaca cgttcggcgc cccctacgtg gtcaaggccg acggcctggc
      781 ggccggcaag ggtgtgctcg taacgcacga ccgggaggcc gccctcgaac acgcgtcgac
      841 gtggctcgtg cacgaggccg tgctcgtcga ggagttcctc gacggccccg aggtctcgct
      901 cttcctgatc agcgacgggc acacggtact gcccctcgcc cccgcccagg actacaagcg
      961 cctcggagac ggcgacgctg gcccgaatac cggcggcatg ggtgcgtact caccgctgcc
     1021 gcgactcgac gagcgcttcg tcgacgaggt gatcgagacg atcgccctgc cgaccgtgcg
     1081 ccaactggcc gaggagcaga ccccgttcat cgggctgttg tactgcggtc tcatccagac
     1141 gcccgccggc atccgggtca tcgagttcaa cgcgcgcttc ggcgacccgg agacgcaggt
     1201 ggtgctgcca cggctggtca cccccctctc gacacttctg ttcgcggcag cgacgggcgc
     1261 cctcgccggc ctcccccggc cggagttcgc gctcgacgtg gccgtcaccg tggtgctcgc
     1321 cagcgagggc tacccgtccg cgccggtcac gggccgcaag atcaccgggc tggatgccgc
     1381 ggcgcaggtt cccgaggtgc agatcctgca cgccgcgacc gccgtcgacc cggccagcgg
     1441 cgcgctgctg gcgaccgggg gccgcgtgct caacgtggtc gccgtgggca cgacgttcga
     1501 cgaggcccgc tcgcgtgcat acgaggcgat gggccgcatt cgtctcgagg gcggtcgata
     1561 ccggtcggac atcgccgcgg gcgtcggcga cgtcacgcgg tagcgcgccg aacggcagcg
     1621 accagcccgg cgacgtcagt ccgtaacgcg ccgaagccgt aacgccacga cgacggtcca
     1681 cgcgaggatc gcggcgatga agacccgctc gctcactccg aagaccccgg gtcgcagccg
     1741 gacggtgagc acgacggccg cgaaagcgac cccggcgatc gccgcaagca cggtcggggc
     1801 aaaggcactc gctgcaggcc ccggccgccc ggcgctcgcg ccccgccgca accatccggt
     1861 cagcacgagc acacccgcca acgccgcggc gaatccgatg ccggccaggg cgaggtggat
     1921 gctcccgtgc accgtgccgc catgcaccgt gccctgcacc ggggcgaccg cgtgcgagcg
     1981 gccggccgtg gcgacatcag tgggaaagaa ggcgagcagg cccgagcaga cc
//
LOCUS       DASTNL010000017         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_570048, whole genome shotgun sequence.
ACCESSION   DASTNL010000017 DASTNL010000000
VERSION     DASTNL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570048"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1677)
                     /gene="groL"
                     /locus_tag="VFQ96_00490"
     CDS             complement(55..1677)
                     /gene="groL"
                     /locus_tag="VFQ96_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039166.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU0256296.1"
                     /translation="MAKIISFDEEARRGLERGLNILADTVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELDDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGADPISLKRGIDKATTAVTEALIADAKEVETKEQIAATASISAADPEIGSII
                     AEAIDKVGKEGVVTVEESNTFGTELELTEGMRFDKGYLSQYFVTDADRQEAVFEDPYI
                     LIANSKISNIKDLLPIVDKVIQAGKQMLIIAEDVEGEALATLIVNKIRGIFKSVAVKA
                     PGFGDRRKAQLQDIAILTGGQVISEEVGLKLENVTLDLLGQARKVVITKDETTIVEGA
                     GDQEQIAGRVQQIRTEIENTDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALIQAGKTAFEKLELVGDEATGANIVKVAIEAPLK
                     QIAQNAGLEPGVVVEKVRGLAVGHGLNAATGEYVDMLDAGINDPVKVTRSALQNAASI
                     AGLFLTTEAVVADKPEKAAAAAPADPSGGMDF"
     gene            complement(1874..2077)
                     /locus_tag="VFQ96_00495"
     CDS             complement(1874..2077)
                     /locus_tag="VFQ96_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0256297.1"
                     /translation="MANGTVKWFNAEKGYGFITVDEGGPDVFVHYSAIDMNGYKVLEE
                     GQRVTFDVGAGAKGPQAESVRLA"
     gene            complement(2206..2862)
                     /locus_tag="VFQ96_00500"
     CDS             complement(2206..2862)
                     /locus_tag="VFQ96_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEU0256298.1"
                     /translation="MQRFPKDRFDVRPRRGERVGVHRSANRSHRGWITVVVSVAAIAV
                     IVGAGFLVVRSPGGLFGLRGAHDSASQATPVTPHTPASSAPARTSAPASTAPQTTAPT
                     TSTPAVGPTVDPNTRLIVLNGTTVPGAAASATTVLENGGWVGVLRPTNASTQNAQGTV
                     IYYTGAEDKGAALGVAQTLSRSGPLAGRQIPVQESGAFAGNGAAITIVLGLDYAGGVG
                     "
     gene            complement(2879..3166)
                     /locus_tag="VFQ96_00505"
     CDS             complement(2879..3166)
                     /locus_tag="VFQ96_00505"
                     /inference="COORDINATES: protein motif:HMM:NF023092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEU0256299.1"
                     /translation="MADSGPEASGSAAAPTDDTVRLLEFERAHRVHDGQKDEAIRDAF
                     GVSAVQYYDRLSAVIDTPAAVRYDPILIRELRATRARLITARQARLFLPRS"
     gene            3368..3799
                     /gene="msrB"
                     /locus_tag="VFQ96_00510"
     CDS             3368..3799
                     /gene="msrB"
                     /locus_tag="VFQ96_00510"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU0256300.1"
                     /translation="MADEMAKTDEEWRENLAPERYAVLRQAATERPWTGELLDEHRAG
                     IYTCAGCGAELFKSGTKFDSDCGWPSFYESVRPEAVTLLEDRSLGMVRTEVRCARCGG
                     HLGHVFDDGFGTPTGNRYCMNSLSLDFAPEAPDAAGTPAAE"
     gene            complement(3880..3952)
                     /locus_tag="VFQ96_00515"
     tRNA            complement(3880..3952)
                     /locus_tag="VFQ96_00515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3917..3919),aa:Thr,seq:tgt)
BASE COUNT          648 a         1466 c         1408 g          750 t
ORIGIN      
        1 gcgggcaccc tcgaaaggag ggtgcccgcc gttcgtcgtg atgcgggtcg ggactcagaa
       61 gtccatgccg cccgacgggt cggccggggc ggccgccgcg gccttctccg gcttgtcggc
      121 gacaaccgcc tcggtcgtca ggaacagccc cgcgatcgac gcggcgttct gcagcgccga
      181 gcgggtcacc ttgaccgggt cgttgatgcc ggcgtcgagc atgtcgacgt actcacccgt
      241 cgccgcgttc aagccgtggc ccacggccag gccgcgcacc ttctcgacca ccacacccgg
      301 ctcgaggcct gcgttctgcg cgatctgctt gagcggggcc tcgatggcga ccttgacgat
      361 gttcgcgccg gtcgcctcgt cgccgacgag ctccaacttc tcgaaggccg tcttgccggc
      421 ctgaatcagg gcgacgccgc cgccagcgac gatgccctcc tctaccgcgg ccttcgcgtt
      481 gcgcaccgcg tcttcgatgc ggtgtttgcg ttccttgagc tcgacctcgg tggccgcacc
      541 cgccttgatg acggccacgc cgccggcgag cttggcgagg cgctcctgca gcttctcacg
      601 gtcgtagtcg ctgtcggtgt tctcgatctc agtgcggatc tgctgcacgc ggcccgcgat
      661 ctgctcctgg tcgccggcgc cctcgacaat ggtcgtctcg tctttggtga tgacgacctt
      721 gcgggcctgg ccgagcaggt cgagggtcac gttctcgagc ttcaggccga cctcttcgct
      781 gatgacctgg ccgcccgtga ggatggcgat gtcctgcagc tgcgccttgc ggcggtcgcc
      841 gaagcccggc gccttgacgg cgacggactt gaagatgcca cggatcttgt tgacgatcag
      901 ggtggccagg gcctcgccct ccacgtcttc ggcgatgatg agcatctgct tgccggcctg
      961 gatcaccttg tcgacgatcg gcagcaggtc tttgatgttc gagatcttcg agtttgcgat
     1021 caggatgtag gggtcttcga agaccgcctc ttgacggtcg gcgtctgtca cgaagtactg
     1081 cgacaggtaa cccttgtcga accgcatgcc ctcggtcagt tcgagctcgg tgccgaaggt
     1141 gttcgactcc tcgacggtga cgacgccctc tttgccgacc ttgtcaatgg cctcggcgat
     1201 gatcgagccg atctcggggt cggccgcaga gatcgacgcg gtggccgcga tctgctcctt
     1261 agtctcgacc tctttcgcat cggcgatgag cgcctcggtc acggccgtcg tggccttgtc
     1321 gatgccgcgc ttgaggctga tcgggtcggc acccgccgcc acgttgcgca gcccctcgcg
     1381 cacgagcgcc tgggcgagca cggtcgcggt cgtcgtgccg tcaccggcga catcgtcggt
     1441 cttcttggcg acctctttga cgagttccgc accgatcttc tcgtacgggt cgtcgagctc
     1501 gatctccttg gcgatcgaga cgccatcgtt cgtgatggtc ggagcgcccc actttttttc
     1561 gagcacgacg ttgcgaccgc gggggccgag cgtcaccttg accgtgtcgg ccaaaatatt
     1621 aaggccgcgc tcgaggcctc gccgagcctc ctcgtcgaac gaaatgatct ttgccatggt
     1681 tgtttctcgt ccctcccgga cgtcgcgaac gatgcaatgc tttagcactc ggctcagacg
     1741 agtgctaaac cgattctggc actctcacgc agggagtgca agcagcaccc acggcacccg
     1801 atagacgaaa acagtcgacg tcatccggaa cggatggcgc cgactgtgcg ctcgactctc
     1861 agtgtgaaat ggattacgcc aggcgcaccg actccgcctg agggcctttc gcgcccgcac
     1921 cgacgtcgaa ggtgacacgc tgaccctctt cgagaacctt gtacccattc atatcaatgg
     1981 cggagtaatg aacgaaaacg tccggtcccc cctcatcgac ggtgatgaat ccgtagcctt
     2041 tttcagcgtt gaaccatttc acggtcccgt tcgccatgta ttactcctgt acttcttgat
     2101 gcgcacagca tggtcgccat gcgtgatgct tgatcatact ggagcgcacg cgtgtggcga
     2161 cagccccagg ccgcaatgat agagaacgtc gacgtcgccc ggtgctcaac cgaccccgcc
     2221 ggcatagtcc agaccgagga cgatcgtgat ggccgcgccg ttacctgcga acgctccgct
     2281 ctcttgcacg ggtatctgtc gcccggccag cgggcccgac cgggagagcg tttgtgcgac
     2341 acccagcgcc gcgcccttgt cctcggcccc cgtgtagtag atcaccgtgc cctgcgcgtt
     2401 ctgcgtgctg gcgttcgtcg gccgcagcac ccccacccag cccccgttct cgagcaccgt
     2461 cgtggcggag gccgcggcgc ccggcacggt ggttccgttc aagacgatca gcctcgtgtt
     2521 cgggtcgacg gtcgggccca cggccggagt gctggtcgtg ggcgcggtgg tctgcggcgc
     2581 ggtggaggcg ggtgccgacg tccgggccgg cgcagaggag gcgggcgtgt gcggcgtcac
     2641 gggggtggcc tgcgacgcgg agtcgtgcgc gccccggagg ccgaagagcc cgcccggcga
     2701 gcgcacgacc aggaatccgg caccgacgat gaccgcgatc gcggcgaccg agacgacgac
     2761 ggtgatccag ccacgatggc tgcggttcgc cgagcggtgc acgcccaccc gttcgccgcg
     2821 ccgaggccgc acgtcgaagc ggtccttcgg gaatctctgc accgtggttg tcctcacttc
     2881 agcttcgggg caagaagagc ctggcctggc gcgccgtgat cagccgggcg cgtgtcgcac
     2941 gcagctcccg gatcaagatc gggtcatacc gcaccgcggc gggggtgtcg atgaccgcgc
     3001 tcagacggtc gtagtactgc acggccgaga ccccgaacgc gtcacggatc gcctcgtcct
     3061 tctgcccgtc atgcacccgg tgagcccgct cgaactcaag gagccgcacg gtgtcgtcgg
     3121 tcggcgccgc ggccgacccc gacgcctcgg gccctgaatc ggccatcgga tcatcccctc
     3181 tgcgcgccag gtcctacccg cggcgggaca cccatgcccg accgcccggc ccatcttatg
     3241 gtgcgcaccc tgcgtgtttc cggcgacgcc ccgcgcgccg accacagccc cctgcgcgcc
     3301 cgcccggccc gaagattcta gagtggtcag ggcgggatgc cacacacaac agcaacaagg
     3361 aggcaccatg gccgacgaga tggcaaagac cgacgaggaa tggcgcgaga atctggcccc
     3421 agagcggtac gcggttctgc ggcaagccgc cacggagcgg ccgtggacgg gcgagctcct
     3481 cgacgagcac cgggcgggca tctatacctg cgcagggtgc ggggcggaac tgttcaagag
     3541 cggcaccaag ttcgattcgg actgcggttg gccgagcttc tacgagtcgg tgcgacccga
     3601 ggcggtcacc cttctcgaag accgtagcct cggcatggtg cgcaccgagg tgcgttgcgc
     3661 acgctgcggc gggcatctgg gacacgtgtt cgacgacggt ttcggaacgc cgaccggcaa
     3721 ccgttactgc atgaactcgc tgtccctcga cttcgcgccg gaggcgcccg acgccgcagg
     3781 cacgccggcc gccgaatagc ccgccgccgg gggccgacga tccgtcaccg tcaccggccc
     3841 ggactgtgcc ggccggtgac gcaggccccg agggtgcaga gccggccacg ggactcgaac
     3901 ccgtaaccac cgctttacaa gagcggtgcg ctgccaattg cgccaggccg gcggtagtga
     3961 catcttacgt gctccgaccc cggcccccct ggaaccagcc ggcggttgac ggtctcgccg
     4021 acgcttctgt gctgcgcgtc acgtgggtgg caacacccac gttgtggagg aggtcggccg
     4081 cgacacgccc cgataccccg gcgtcggggg tcatccggcg gcgtgcggcg gcgaagacgg
     4141 cttcgtgctc gtcggagacg gcagcggcgg ggcggaggaa ggcatcggca gcggcgtcac
     4201 gttcgaagcc tggtagctct ctcccgaggc ttgcagcacg aaggccttga actgcgcgac
     4261 atcggtcagc ga
//
LOCUS       DASTNL010000018         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1311978, whole genome shotgun sequence.
ACCESSION   DASTNL010000018 DASTNL010000000
VERSION     DASTNL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311978"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VFQ96_00520"
     CDS             complement(<1..1878)
                     /locus_tag="VFQ96_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEU0256301.1"
                     /translation="MRIPENIRRTLDTLTRQQKVSLTSGASFWRTKPVPEASVPALVL
                     TDGPHGVRMQAGDGEHLGLTESVPATCFPPAAALASSWDPALVRRVGEALGEEARAEG
                     VQVLLGPGINIKRSPLCGRNFEYLSEDPYVAGVLGAALVQGIQQRGVAASLKHFAANN
                     QETDRLRVSADVDERTLREVYLRGFQRVVAKARPWTVMCSYNGINGVRVSEDRWLLTT
                     VLRDEWGFDGLVVSDWGAVVDRAAALEAGVDLEMPSRAAADMELTAALDAGRVSEAAL
                     DQAAARVLTLIERTTPAAGSSSPDADGYDRVAHHALARETAARCVVLLKNEGDLLPLD
                     PDTNVAVIGEFARTPRYQGSGSSQVVPTRLDTALDEIRRIAGRDVPFAPGFALPGAPT
                     GDGGPTAGGASAGGSLLDEAVAVARAAETVVLFLGLPASDESEGFDREHLNLPPEQTA
                     LLDAVLAVNSTVIVVLANGGVVRVSGWDERVPAILEGWLLGQAGGGAIADILFGRVEP
                     SGRLAETIPARLEDTPAYLNFPGENYHVRYGEGLFVGYRWYDARDLAVSYPFGHGLSY
                     TQFDYGDVVVEAVGSGDTVAPTGVAGSAEAVGSAEVAGPGGPAEPGDAVPPAGGAGAD
                     GL"
     gene            complement(2007..3230)
                     /gene="fdhA"
                     /locus_tag="VFQ96_00525"
     CDS             complement(2007..3230)
                     /gene="fdhA"
                     /locus_tag="VFQ96_00525"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016997985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEU0256302.1"
                     /translation="MAANRAVAYKGPRHVEVIDTDYPTFELKDGPGVNPANIGRKVPH
                     GAIVRTVATNICGSDQHMVRGRTTAPSDLVLGHEITGEVVEVGPDVEFVKVGDLVSVP
                     FNIACGRCKNCIERKTGICLNVNPDRPGSAYGYVDMGGWVGGQANYVLVPYADFNALV
                     FPDKDQAMEKILDLAMLSDIFPTGYHGAYSAGVTTGSTVYVAGAGPVGLAAATASLLL
                     GASAVIVGDMNENRLKQARSFGCETVDLTKGGPVEQIEQILGVPEVDCAVDAVGFEAK
                     GHGAGAKEAPATVLNSLMDITAAGGSLGIPGLYVTGDPGGVDEAARVGALSLSLGTGW
                     AKSLAFTTGQCPVMKYNRGLMKAILADRVHIAKNVNAQVIGLDDAPQGYDAFDKGAAT
                     KFVLNPNGYLDKLAA"
BASE COUNT          544 a         1253 c         1176 g          509 t
ORIGIN      
        1 caacccgtcg gcaccggccc cgcctgccgg cggaaccgcg tcgccaggct ctgccgggcc
       61 acccggcccg gcaacctcag ccgagccgac cgcttcagca gacccggcca ctccggtagg
      121 cgcgaccgtg tcgcctgagc cgacggcctc gacgacgaca tcgccgtagt cgaactgcgt
      181 atagctcagg ccgtgtccga acgggtacga gacggcaagg tctcgggcgt cgtaccagcg
      241 gtagccaacg aagagcccct ccccataccg cacgtgatag ttctcgccgg gaaagttcag
      301 gtaggcggga gtgtcttcga ggcgagccgg gatcgtctcg gcgagccggc cggagggctc
      361 gacgcggccg aagaggatgt cggcgatcgc gccgccgccg gcctgcccga gcagccagcc
      421 ttcgagaatc gccggcaccc gctcgtccca gcccgagacc cgcacgacgc caccgttggc
      481 gagcacgacg atgacggtgc tgttgacggc cagcacggcg tcgagcagcg ccgtctgctc
      541 gggcggcagg ttcaggtgct cccgatcgaa gccctccgac tcgtcgctcg ccggcaagcc
      601 gaggaagagc acgacggtct ccgccgcgcg ggcgaccgcg accgcctcgt cgaggaggct
      661 gccacccgca gacgccccgc ccgccgtggg gcccccgtca cccgtcggcg cgcccggcag
      721 cgcaaaaccg ggggcgaacg gcacgtctcg gcccgcgatg cggcgaatct cgtcgagcgc
      781 ggtgtcgagc cgggtcggca cgacctgcga actgccggag ccctgatagc gcggcgtgcg
      841 ggcgaactcc ccgatcaccg ccacgttcgt gtcggggtcg aggggcaaga ggtcgccctc
      901 gttcttcaga agcaccacgc aacgcgcagc cgtctctcgg gccagcgcgt ggtgcgccac
      961 ccggtcgtaa ccgtcggcgt cgggcgacga ggaaccggcg gccggcgtcg tgcgctcgat
     1021 gagtgtcagc acgcgcgccg ccgcctggtc gagggcggcc tcggagacac ggccggcgtc
     1081 gagcgccgcc gtcagttcca tgtcggcggc ggcacgggac ggcatctcca ggtcgacacc
     1141 ggcctcgagc gcggcggccc ggtcgacgac cgcaccccag tcggagacga caagcccatc
     1201 gaagccccac tcgtcgcgca gcaccgtcgt caaaagccac cggtcctcgc tgacccgcac
     1261 cccgttgatg ccgttgtacg aacacatcac cgtccacggc cgagccttcg cgacgacgcg
     1321 ctgaaacccc cgcaggtaga cctcccgaag ggtgcgctcg tcgacgtcgg cgctgacccg
     1381 cagccggtcg gtctcctggt tgttggcggc gaaatgcttg aggctggccg cgaccccgcg
     1441 ctgttggatg ccctgcacga gggccgcgcc gagaacgcct gcgacatacg ggtcttcgga
     1501 gaggtattcg aagttgcgtc cacacagggg gctgcgtttg atgttgatgc cgggccccag
     1561 gagcacctgc acgccctcgg cacgggcctc ttcgcccagg gcctcgccga cgcgacgcac
     1621 cagggccgga tcccagctgg aggccagggc ggccgcgggt ggaaagcagg tggcgggcac
     1681 gctctcggtc aagcccagat gctcgccgtc gccggcctgc atgcgcacgc cgtgcgggcc
     1741 gtcggtgaga acgagggccg gcaccgacgc ctcgggcacc ggtttcgtgc gccagaacga
     1801 tgccccgctg gtgagcgaca ccttctgctg tcgggtgagc gtgtcaagcg tgcggcgaat
     1861 gttctcggga atgcgcatcg tcgccatcct tccacctcgc catcgcgcgg ggcagcaccc
     1921 gcagcagacg ttcgaggcgg ccgacgccaa gcgccgaccg cccctcacct actcctaggc
     1981 cgaaagagcc caggcccgtg cccccgtcaa gcggcgagct tgtcgaggta gccgttcggg
     2041 ttcagaacga acttcgtcgc ggcgcccttg tcgaacgcgt catagccctg cggcgcgtcg
     2101 tcgaggccta tgacctgcgc gttgacgttc ttcgcgatgt gcacccgatc ggcgaggatc
     2161 gccttcatga gcccccggtt gtacttcatg accgggcatt ggccagtggt gaacgccagc
     2221 gacttcgccc agccggtgcc gaggctgaga gagagggcgc cgacccgggc agcctcatcg
     2281 accccgcccg ggtcgcccgt gacgtagagg cccgggatgc cgagcgaccc gcccgccgcg
     2341 gtgatgtcca tcaacgagtt cagcaccgtc gccggcgcct ctttcgcccc cgcgccgtgg
     2401 cccttcgcct caaaaccgac cgcgtcgacc gcgcagtcga cctcgggcac gccgagaatc
     2461 tgttcgatct gctcgacggg cccacccttg gtcaggtcga ccgtctcgca gccgaagctc
     2521 ctggcctgct tgagtcggtt ctcgttcatg tcgccgacga tgacggccga ggcgcccaga
     2581 agcagcgacg cggtcgcggc agcgaggccc accgggccgg cacccgccac gtacaccgtc
     2641 gagccggtgg tcaccccggc gctgtacgcg ccgtggtagc cggtcgggaa gatgtcggag
     2701 agcatcgcca ggtcgagaat cttctccatc gcctggtctt tgtcggggaa caccagggcg
     2761 ttgaagtcgg cgtacggcac gagcacatag ttcgcctggc cgcccaccca gccgcccatg
     2821 tcgacgtagc cgtaagcgct gcccggccgg tcggggttga cgttcagaca gatgcccgtc
     2881 ttgcgctcga tacagttttt acagcgccca caagcgatgt tgaacggcac cgagacgagg
     2941 tcgccgacct tcacgaactc gacatccggg ccgacctcca ccacctcccc cgtgatctca
     3001 tgcccgagaa cgaggtctga cggggcggtg gtgcggcctc gaaccatgtg ctggtcagac
     3061 ccgcagatgt tggtggcgac ggtgcgcacg atggcgccgt gcggcacctt gcgcccgatg
     3121 ttggcggggt tgacccccgg gccatccttc aactcgaagg tggggtagtc cgtgtcgatc
     3181 acttcgacgt gtcgaggtcc cttataggcg acggctcggt ttgcagccac gacatctctc
     3241 ccttgactcg cgcgatggat tgcgcccgga gcgccggggg atgaacgacc gacacgcacg
     3301 ccggccggat gcgcaccccg ggaaccaggc tcttgcccgg aggctacccg gggcccccaa
     3361 ggccgacaag ggcctcaacc tggcagggtc gtgagtgctc tctcagtgtt cgctctcgcc
     3421 ggcgtcgggc gtcgggcgat gccgtgccaa gaggtccaga agggcggccc cataggccga
     3481 tc
//
LOCUS       DASTNL010000019         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_199997, whole genome shotgun sequence.
ACCESSION   DASTNL010000019 DASTNL010000000
VERSION     DASTNL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199997"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3017)
                     /locus_tag="VFQ96_00530"
     CDS             complement(<1..3017)
                     /locus_tag="VFQ96_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256303.1"
                     /translation="MAEPSLDAPRPQGWLRRLGGYLLRHRRDVIVSLVAAVLGSGIKV
                     ITPLIARVVVDDVVAHKTGGILWWIVALLALAVIGFGFAYLRRFRAGRAALDVQNDLR
                     REMHDRLQGMDAATLDTMPTGQLVARANSDTGLVLGLLNFYPLVSGNVLMMLLSLVVM
                     FVLSPLLALISLAVLPALLIVSYRMRSRVFPASWDGQQKEGDLAGIVDEDIAGVRVVK
                     AFGQEGRELRRLADSASALYGSQMRTTRIQARFQPMLEAIPQLGQVGILALGGLMALH
                     GELTLGTFLAFSSYIAQLMAPARQLAGILTIAQQARAGVERIFQLLDRKPAIVDEPGA
                     VDLPEAADPGVPLDAAGEIAFRDVRFTYPGASRPVLDGLTLTVRPGERVALVGASGSG
                     KSTIIRLLARYYEADAGTLTVGGVDVRRLRLGSLRRAVGLTFEDSFLFSESVRANIAY
                     GDPAANDEAVVRAARAAQADDFIRALPHGYDTVVGERGLSLSGGQRQRIALARVLLRD
                     PRILVLDDATSAIDARTEQAIHDGLRREMAGRTVLIVAHRHSTLHLADRIVVVEGGRV
                     ADEGTHDELIERSARYRALFLGEDDGAEEGDVDALVGAAARVATTESAAGARPAAGAG
                     RTVSLGAGLGRGGGGWRKSLAPTPELLALVDRLEPVKDVPEIDLERESRRQAHFSLGG
                     LLREFRKPLALALVFVVIDALSGLVGPALVKAGVDQGVVGGSTGVLYLASGLYLAIVA
                     VALANEIGEVFVTGRVAQRIMLSLRIRIFAQLQRLSLDYYEREMAGRIMTRMTTDVDQ
                     FDQLLENGLLGALVAIVTFVGVAVALVVLNVPLGLATLAVVIPLIVATFFFRRRASRL
                     YTLSRERIAVVNAAFQEGLSGVREAQAFTHEDATIHRFAGLCGSYLESRVAAQRLVAV
                     YFPFVQFLSSVADAIVLGLGAYLIATGHVTTGTLIAFLLYIDMFFSPIGQLSQVFDSW
                     QQTRVSVGRIAGLMRLDTLTPEPRRPVAVPSLAG"
BASE COUNT          543 a         1058 c         1177 g          406 t
ORIGIN      
        1 ccggcgaggc ttggcacggc caccggccta cgaggctccg gggtgagcgt gtcgagcctc
       61 atgagccccg cgatgcgccc gaccgacacg cgggtctgct gccaggagtc gaagacctgg
      121 gagagctgcc cgatgggcga gaagaacatg tcgatgtaca gcaggaacgc gatgagggtt
      181 cctgtcgtga cgtgcccggt ggcgatgagg taggcgccga ggcccagcac gatcgcgtcg
      241 gcgacggagg aaaggaactg cacgaacggg aaatagacgg cgacgagccg ctgcgccgcg
      301 acccgcgatt cgagatagct gccacacagc ccggcgaagc ggtggatcgt cgcgtcttcg
      361 tgggtgaacg cctgcgcctc gcgcaccccg gacagtccct cctggaaagc ggcgttcacg
      421 acggcgatcc gctcccgcga cagggtgtag aggcgggagg cgcgcctgcg gaagaagaag
      481 gtcgcgacga tgagcgggat gacgacggcc agggttgcga ggccgagcgg cacgttcagc
      541 acgacgaggg cgacggcgac gccgacgaag gtgacgatcg cgacgagcgc gcctaggagc
      601 ccgttctcta aaagctggtc gaactggtcg acatcggtcg tcatccgggt catgatgcgc
      661 ccggccatct cacgctcgta atagtcgagc gagaggcgtt gcagctgcgc gaagatgcgg
      721 atgcgcaacg agagcatgat gcgctgcgcc acccgaccgg tgacgaagac ctcgccgatc
      781 tcgttggcga gcgcgaccgc gacgatggcg aggtacaggc cggaggcaag gtagagaacc
      841 ccggtgctgc cgcccacgac cccctggtcg acgccggcct tgacgagcgc cgggccgacg
      901 agccccgaca gggcgtcgat gacgacgaag acgagggcga gagcgagagg cttgcggaac
      961 tcccgcagaa ggccgccgag cgagaagtgc gcctgccggc gcgactcccg ctcaaggtcg
     1021 atctctggca cgtctttcac cggttccagg cgatcgacga gcgctaagag ctccggtgtg
     1081 ggggcgaggc tcttgcgcca cccaccgccg ccgcggccga gaccggcgcc gagcgagacg
     1141 gtgcggccgg ccccggcggc agggcgtgcc ccggcggcgg attccgtggt ggcgacacgc
     1201 gcggcggcgc cgaccagggc atccacgtcg ccctcctcgg caccgtcgtc ttcgcccagg
     1261 aacagcgcgc gataccgtgc gctgcgttcg atcagctcgt catgggtgcc ttcgtcggcg
     1321 acgcgaccgc cctcgacgac gacgatccgg tcggccaggt gcagcgtgga gtgccggtgg
     1381 gcgacgatca agacggtgcg cccggccatc tcccgccgca gtccgtcgtg gatcgcctgc
     1441 tcggtgcggg cgtcgatcgc gctcgtcgcg tcgtctaaga cgaggatgcg ggggtcgcgc
     1501 aagagcaccc gggccagggc gatgcgctgg cgctgcccgc ccgacaggct gagcccgcgc
     1561 tcccccacaa ccgtgtcgta gccgtgcggc agcgcccgga tgaagtcgtc cgcctgcgcg
     1621 gcgcgtgccg cccgaacgac cgcctcgtcg tttgccgccg ggtcgccgta cgcgatgttc
     1681 gcccgcaccg attcggagaa gagaaagctg tcttcgaagg tgagcccgac cgcccgacgc
     1741 aaggagccaa ggcgaaggcg acgcacgtcg acgccgccga cggtgagcgt gccggcgtcc
     1801 gcctcgtaat atcgggccaa gaggcggatg atcgtggatt ttccgctgcc gctcgccccc
     1861 accaacgcga cccgctcgcc cgggcgcacg gtgagcgtga ggccgtcgag cacggggcga
     1921 gaggccccgg ggtacgtgaa gcgcacgtcg cggaaagcga tctcgccggc cgcgtcgagc
     1981 ggcacgccgg gatcggcggc ctcgggcagg tcgaccgcgc ccggctcgtc gacgatcgcc
     2041 ggcttgcggt cgagaagctg aaagatgcgc tccacgccgg cgcgtgcctg ctgggcgatg
     2101 gtgaggatgc cggccagctg tctcgccggc gccatcaact gggcgatgta gctggagaag
     2161 gcgaggaacg tgccgagcgt gagctcgccg tgcaacgcca tgaggccgcc cagcgcgagg
     2221 atgccgacct ggccgagctg cgggatcgcc tcgagcatcg gctgaaagcg ggcttggatg
     2281 cgggtggtgc gcatctgcga gccatacagc gccgacgcgg agtcagccag ccgacgcagc
     2341 tcccggccct cctggccgaa ggccttcacg acgcgtacgc cggcgatgtc ttcgtcgacg
     2401 atgccggcca ggtcgccctc tttctgctgc ccgtcccacg aggcggggaa cacgcggctg
     2461 cgcatgcggt agctgacgat caggagggcg ggcaagacgg ccaggctgat aagggccaag
     2521 agaggcgaga gcacgaacat gacgacgagc gaaagcagca tcatgagcac gttgccgctg
     2581 acgagcgggt agaagttcag aagtcccaag acgagcccgg tgtcggagtt ggcgcgggcg
     2641 accaactggc cggtcggcat ggtgtcgagc gtcgcggcat ccataccctg cagccgatcg
     2701 tgcatctcgc gacgcaggtc gttctgcacg tcgagcgcgg cacggccggc gcggaaccgg
     2761 cgcaggtagg cgaacccgaa gccgatgacc gccagggcga ggagcgccac gatccaccag
     2821 agaatgccgc cggtcttgtg cgcgaccacg tcgtcgacca cgacccgggc gatgagcggg
     2881 gtgatcacct tgatgccgct gccgagcacg gcagcgacga gggagacgat cacgtcgcgg
     2941 cgatgccgca gaaggtagcc gcccagccga cgcagccagc cctgtgggcg cggcgcatcc
     3001 agggagggtt ctgccaccga tccacagtat cgccgcgccg cccagcgtgc cgcggaccgg
     3061 accggaccgg gccgccgggc gccgggcgcc aggccttcgg tacggccggg cggcatcgat
     3121 cggcgggtca cagcgccggg cgtcggcggc tatcgtcgtt gtgagcatcg tctcggcgac
     3181 cgag
//
LOCUS       DASTNL010000020         6656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1498668, whole genome shotgun sequence.
ACCESSION   DASTNL010000020 DASTNL010000000
VERSION     DASTNL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498668"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFQ96_00535"
     CDS             <1..554
                     /locus_tag="VFQ96_00535"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256304.1"
                     /translation="DLFDAAKLVGTIANRSAAQQINHWARIGRELEASPGTSLRDIRR
                     VLPGEASYDDFGEHSQAVMRAIWDEQMAEGRARLNLAIEAAARRYLVHAHVILVPVDL
                     AVTRVAERVQNGGHPVPERKVRERYARLWSLVTEARSVTDRMEFFDNSVARHPFQPVA
                     TYEHGLPTSNPDWPTWAPAALTA"
     gene            648..1124
                     /locus_tag="VFQ96_00540"
     CDS             648..1124
                     /locus_tag="VFQ96_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0256305.1"
                     /translation="MAVSQRMSIQDVDYGAYKAVFPLQKYISSGGIDEKLLLLIQIRS
                     SQLNQCAWCLDMHLTEARKQGIPQRKLDLIAAWREAPSQFTDEERAILAFTEQVTLIS
                     QGGVSDEVWADLRSHFDEKKTLTLLMAVAAINVWNRMNVTVHSDLAPAGEPVAAAA"
     gene            1143..2357
                     /locus_tag="VFQ96_00545"
     CDS             1143..2357
                     /locus_tag="VFQ96_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256306.1"
                     /translation="MTTAGTREGAGRPSLPRRWAFAGAAYMFGVTMLGTTLPTPLYPS
                     YETRYGFGSLTTTVVYAAYAAGVLAVLLLAGSASDVVGRRPLLLAGLAASVFSAVAFA
                     TDSSLVMLYVGRVFSGLSAGIFTGTATVAMVELAPARRKRQASIVASAVNMLGLGLGP
                     LVTGVAATTLPAPLRTPYLLDLVLLLPALAAVWFLPETVPHPVRRLPTPRRPGVAAEA
                     RGVFVPAATVAFAAFAVFGLVTAIAPAFLVTLLHLPSPLLSGALVFAMFAGSALGQLT
                     ITPLIRRGTLLLGSAILLAGLAAIGASLATESLATLFAGTIAVGIGQGVSFSAGIAAI
                     SAASPAGRRAETVSMFFVVAYAAISLPVVLVGVAADAWGLRLAGIVFAAVMAALTLAA
                     GTAVAVRALRHS"
     gene            2488..3762
                     /locus_tag="VFQ96_00550"
     CDS             2488..3762
                     /locus_tag="VFQ96_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256307.1"
                     /translation="MTGGVTNGHDARPHPHDPGGTPAGGGWPGHPAGSTGYRRIVAAL
                     FLGGIATFAQLYSPQAALPEIGTDLHIGAATSSLMISAATLGLAAGVIPWSAVADRFG
                     RVRAMATAMISATVFGLAVPFLPQLWMILTGRFLEGLFIGGVPAIAIAYLTEEVDRRH
                     AARVAGMYVAGTSLGGLTGRLIAGPVADAAGWRAAVFLVALISALAAAGFVAMAPAAR
                     GFTPGRTRAWAGLARRLAGNLRSPTQLALYAQGLTLMGGFVAMYNYLTFRLTSPPFSL
                     PVSVTSLIFLAYLAGTWSSTRAGALASRIGRLPVLAGSATLFAAGVAVTLIDWLPAVL
                     AGLVVATAGFFGAHSVASGWTGAAARVGRAQASSLYNLFYYVGSSVFGWAGGLFFSWA
                     GWPGATAMIVALALVAIGTALAVLRHATTRAA"
     gene            4185..5723
                     /locus_tag="VFQ96_00555"
     CDS             4185..5723
                     /locus_tag="VFQ96_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256308.1"
                     /translation="MASTTSAQHPAIRGRRAAAPTPHDPSDDDRATREPAAPDAFTLG
                     RRIRQARLERGLTLGELGDAVGRAASQISVIETGKREPSLSMLRSLAGALGVTTGQLL
                     DPEPPSERAALEIALERAQHGAVFASLGIAPMRISRSLSDETLHTILALHHEVERLHR
                     ERAATPEEARRENTKLRATMRERNNYFPELEKLATDLLSAVGHSGGPIGQQTVLDMAD
                     HLGYSLHYVGDLPHSTRSVTDNRNGRIYLPTEQSVSRDSRSPILQAFASRICEHEEPT
                     SYADFLRQRVETNYLTAAILLPEDDAVRMLAEAKKNRRISLEDLRDAFAVSYETAAHR
                     FTNLATARLDLPVHFTKVHESGTITKAYENDAVHYPSDATGAIEGTQVCRRWTAFTVF
                     KATDRFSPWYQYTDTPNGTYWCTSRIEKAKEGQYSVSVGVRFEDAKFFRGRETTHREA
                     STCPDERCCHLPPADLAAKWGETAWPSARTPTSLLAALPTGAFPGVDTTEVYEFLERH
                     QPAD"
     gene            5774..6247
                     /locus_tag="VFQ96_00560"
     CDS             5774..6247
                     /locus_tag="VFQ96_00560"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0256309.1"
                     /translation="MTRRWRLSSSIEIARDAQTLYQMVADVTRMGEWSPVNTGGRWES
                     DERGVGALFTGRNETPGRTWETHCEVIVATPGREFAFAVGGARTRWGYTFEKTPSGTR
                     VTESWEVPEESMPHWQERFGETTQKELGIREQAAREGMPATLAALKRVAEAAPAA"
     gene            complement(6213..>6656)
                     /locus_tag="VFQ96_00565"
     CDS             complement(6213..>6656)
                     /locus_tag="VFQ96_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256310.1"
                     /translation="AGTLTTGALLQRLRNQSGLAAVLALFSLIGVLGQLALPALAVVW
                     VSFIGFSAGGAIVVGLSLFGLRTRDHRRAAALSGMAQSVGYLIAASGPIVLGTLHDLT
                     GSWRMPLFVLVGVCVAEGVFAILAGRDRLVAEVPVTRRARPQLRV"
BASE COUNT         1016 a         2423 c         2211 g         1006 t
ORIGIN      
        1 gcgatttgtt cgacgcagcg aagctcgtcg gcacgatcgc caaccgtagc gccgcgcaac
       61 agatcaacca ctgggcgcgg atcgggcgtg agctcgaagc ttcgcccggc acgagccttc
      121 gcgacattcg acgcgtgctc ccgggcgaag cctcctacga cgatttcggc gaacacagcc
      181 aagcggtgat gagggcgatc tgggacgagc agatggccga aggtcgcgcc cgtctcaacc
      241 tcgccatcga ggcggcggca cgtcgatacc tcgtccacgc acacgtcatc ctcgtccccg
      301 tcgatctggc cgtgacgcgg gtcgccgaac gggtgcaaaa cggtgggcac cccgtaccgg
      361 aacgcaaggt ccgcgagcga tacgcacggt tatggagcct cgtgaccgag gcccgctctg
      421 tgacagaccg catggaattc ttcgacaaca gcgtggcccg ccaccctttc cagcccgtcg
      481 cgacatacga acacgggctg ccgacaagca accccgactg gcccacgtgg gccccggccg
      541 cactcaccgc ctgacgcggg gagcggcacg gcgccacgag cattctcggc acaaacatcg
      601 cccggcgaaa taccatcgaa gaagcagata tcggtaaagg agattccgtg gctgtttcgc
      661 aaagaatgtc gattcaagac gttgactatg gcgcctacaa ggcggtcttc ccgctgcaga
      721 agtacatttc gtcgggcggc atcgacgaga agctgctgtt gctcatccag attcgttcct
      781 cacagctgaa ccagtgcgcc tggtgcctcg acatgcacct gacggaggcc cgcaagcagg
      841 gcatcccgca gcgcaagctc gacctcatcg ccgcctggcg ggaggcgccg agccagttca
      901 ccgacgagga gcgcgccatc ttggccttca ccgaacaggt gacgctcatc agccagggcg
      961 gtgtctccga cgaggtgtgg gcagacctgc gctcgcactt cgacgagaag aagaccctca
     1021 cgctgctgat ggcggtcgcc gcgatcaacg tgtggaaccg gatgaacgtg acggtgcact
     1081 ccgacctcgc gcccgcgggc gagccggtcg ccgcagccgc ctgatacgtg acgaccgaat
     1141 cgatgacgac ggccggtacc cgtgagggtg ccggccgtcc ctcgttgccg cgccgctggg
     1201 cgttcgccgg tgcggcctac atgttcggcg tcacgatgct gggcacgacc ctgccgacac
     1261 cgctctaccc ctcgtacgag acgaggtacg ggttcggcag cctgacgacg acggtcgtct
     1321 acgcggcgta cgccgccggc gtgctggccg tgttgctcct ggccggcagc gcctcggatg
     1381 tcgtcgggcg gcggccgctt cttctggccg gcctcgccgc ctccgtcttc tccgccgtgg
     1441 ccttcgcgac ggattccagc ctcgtcatgc tgtatgtggg gcgagtgttc tccggcctct
     1501 cggcggggat cttcacgggc acggcaactg ttgcgatggt cgagctcgcg cccgcccggc
     1561 gcaagcggca ggcgagcatc gtcgccagcg ccgtgaacat gctgggcctc gggctcggcc
     1621 cgctcgtcac cggcgtcgct gccaccaccc tgccggcgcc gctgcgcacg ccctacctcc
     1681 tcgacctcgt gcttctcctg ccggccctcg cggcggtctg gtttctgccc gagaccgtgc
     1741 cgcacccggt gcggcgcttg cccacgcccc gccggccggg cgtcgcggcc gaggcgcgcg
     1801 gcgtcttcgt gccggccgcg accgtcgcgt tcgccgcgtt cgcggtcttc ggcctcgtga
     1861 cggcgatcgc gccggcgttc ctcgtcactt tgctgcatct gccgagcccg ctcctctccg
     1921 gcgcactcgt gttcgccatg ttcgccggct ccgccctcgg ccaactgacg ataactcccc
     1981 tcatacgccg gggcacgttg ctcctcggtt ccgcgatcct gctggccggc ctcgccgcga
     2041 tcggcgcctc cctcgcgacc gaatcactgg cgaccctctt cgccggcacg atcgcggtcg
     2101 gcatcgggca gggcgtcagc ttcagcgccg gcatcgccgc gatctcggcc gcgagccccg
     2161 ccggccggcg ggcggagacc gtctcgatgt tcttcgtcgt cgcctacgcg gcgatctccc
     2221 tgcccgtcgt gctcgtcggt gtcgcggcgg atgcctgggg gctgcggctg gcgggtatcg
     2281 tcttcgccgc ggtcatggcg gcactcacgc tcgccgccgg aaccgccgtc gccgtgcgcg
     2341 ccctccgcca ctcgtgagac ccgccgtctg gggtcacacc ggaaacggcc acgcttgcgc
     2401 gagtgaacag gcggcgctac ggcgaccgag actcacactg cgggcgcacg cggaggcgtc
     2461 cgcgccggta ggccgtagcg tggaagtgtg acaggcgggg tgacgaacgg tcatgacgca
     2521 cggccacacc cgcacgaccc gggcggcacg cccgcgggcg gcggatggcc cggccatccg
     2581 gccggcagca caggctaccg gcgcatcgtc gccgcgctct tcctcggcgg catcgccacg
     2641 ttcgcccagc tgtactcccc gcaagcggcg ctgcccgaga tcggcaccga cctgcacatc
     2701 ggcgccgcca cctcctcgct catgatctcc gcggcgacct tggggctcgc cgccggagtc
     2761 ataccgtggt cggctgtcgc cgaccggttc gggcgcgtgc gggcgatggc gaccgcgatg
     2821 atctcggcga ccgtcttcgg cctggcggtg ccgttcttgc cgcagctctg gatgatcctc
     2881 accggacgct ttctcgaagg actcttcatc ggcggggttc cggcgatcgc gatcgcctac
     2941 ctcaccgaag aagtggatcg ccgtcacgcc gcacgcgtgg cgggcatgta cgtcgcgggc
     3001 acgtcgctcg gcgggctcac cggtcggctg atcgcgggcc ccgtggcgga tgccgccggc
     3061 tggcgggcgg ccgtgttcct cgtcgccctc atctctgccc tggccgccgc cggtttcgtc
     3121 gccatggcac ccgccgcccg cggcttcaca ccgggccgca cccgcgcgtg ggctggtctc
     3181 gcgaggcgcc tggccggcaa cctgcgctcc cccacccagc tcgccctcta cgcgcaaggc
     3241 ctcaccctga tgggcggctt cgtcgcgatg tacaactacc tcacgttccg cctgacctcg
     3301 ccgccgttct ccctgcccgt ctcggtgacg agcctcatct ttctcgccta cctcgccgga
     3361 acgtggtcgt cgacgagggc gggcgcgctc gcctcccgca tcggccggct gccggtgctc
     3421 gccggcagcg ccacgctctt cgcggccggc gtcgccgtga cactcatcga ctggctgccg
     3481 gcggtgctcg ccggcctggt cgtcgcaacg gccggcttct tcggggccca ctcggtggcc
     3541 tccgggtgga ccggggcggc agcccgcgtg gggcgggcgc aggcctcctc gctgtacaac
     3601 ctgttctact acgtcggatc gagcgtgttc ggctgggctg gcgggctttt cttctcgtgg
     3661 gccggctggc cgggcgccac cgcgatgatc gtcgccctgg ctctcgtcgc catcggcacg
     3721 gccctggccg tgctgcgtca cgccacgacg cgggccgcat agggagggca gactatcggt
     3781 gccagccgaa gccctcacac gaaggatgcg agcgccgcgg ccctgcgcac tgcgtgcaaa
     3841 gaaacaccgg caccggccct gtggggccga tgccggtcga gatcgtctcg cgccgcgaca
     3901 tcgtcagtcg cgcggaatag ccacccagcg ggggtcggcg tcccaatcga tcgaaagctc
     3961 gtcgacgccc gtgtacccga tggtctcctg agtctgcgtt gatgacgcga tcatggtgat
     4021 tcctcctcgc tgtttctcgt tccgatctgc ggaggctggt gccatccgca gggggtgata
     4081 tatcaatggt gacggaccag ggtcgggaat cgcgcaaatt tctgaacaga aatccggtca
     4141 atcttctgct tgtaagaaat accatcgaat acgacactgg aagcatggct tcgacgacgt
     4201 ccgcacagca cccagcgata cgcggccggc gcgcggccgc gccgacgccg cacgacccct
     4261 ccgacgacga ccgggcgaca cgggagcccg ccgcacccga cgcgttcacc ctcggccgtc
     4321 ggatccgcca ggcgcggctg gagcgcggcc tcacgctcgg cgagctgggg gatgcggtcg
     4381 gccgggccgc ctcacagatc tccgtcatcg agacgggcaa gcgagagccc agcctgtcga
     4441 tgctacgttc cctcgccggc gccctcggcg tgaccaccgg gcagctgctc gaccccgaac
     4501 cgcccagcga acgcgcggcg ctcgagatcg cgctggagcg agcgcagcac ggcgccgtct
     4561 tcgcctcgct cggcatcgcg ccgatgcgta tctcccgctc actctccgac gagacgttgc
     4621 acacgatcct ggcactgcac cacgaggtgg agcggctgca ccgcgagcgc gccgccaccc
     4681 cggaggaggc ccgccgcgag aacacgaagc tgcgcgccac gatgcgggag cgcaacaact
     4741 attttcccga gttggagaag ctggccacag acctcctttc cgcggtcggc cacagcggcg
     4801 ggccgatcgg gcagcagacg gtgctcgaca tggccgacca cctcggctac tccctgcact
     4861 acgtcggcga cctgccgcat tccacccgat cggtgaccga caaccgcaac ggccgcatct
     4921 acctgccgac cgagcagtcc gtctcgagag actcccgctc ccccatcttg caggcgttcg
     4981 ccagccgcat ctgcgagcac gaagagccga cgagctacgc cgactttcta cggcagcgag
     5041 tggagacgaa ctacctgacg gcggcgatcc tcttgcccga agacgatgcc gtgcggatgc
     5101 tcgccgaggc gaagaaaaac cgccgcatct cgttggaaga cctgcgcgac gcgttcgccg
     5161 tctcgtacga gacggccgcg caccggttca cgaacctcgc gacggcccgg ctcgacctgc
     5221 cggtgcattt cacgaaggtg cacgagtcgg gcacgatcac gaaggcgtac gagaacgacg
     5281 ccgtgcatta cccctccgac gcgacgggcg cgatcgaggg cacgcaggtg tgccgacggt
     5341 ggacggcgtt cacggtgttc aaggcgaccg accgtttcag cccgtggtat cagtacaccg
     5401 acacaccgaa cggcacctac tggtgcacct cccgcatcga gaaggcgaag gagggccagt
     5461 actcggtgag cgtcggcgtg cgcttcgaag acgcgaagtt ctttcgcggg cgggagacga
     5521 cgcaccggga ggcctccacc tgccccgacg aacgctgctg ccacctgccg cccgccgacc
     5581 tggccgcgaa gtggggcgag acggcgtggc cgagcgcccg tacccccacg agccttctcg
     5641 cggcccttcc gaccggggct ttcccgggcg tggacacgac cgaggtgtac gagttcctcg
     5701 agcgacacca gccggccgac tgaccggcaa cccggcctgc cgcctcgccg gccggcgtcg
     5761 atagactcgc cccatgacac gacggtggcg gctttcgagc tcgatagaga tcgcgcgcga
     5821 cgcacagact ctttaccaga tggtggcaga cgtgacacgc atgggcgaat ggagcccggt
     5881 gaacaccggt gggcggtggg agagcgacga acgtggcgtg ggcgccttgt tcaccggccg
     5941 caacgagacg cccggtcgaa cctgggagac gcactgcgag gtcatcgtcg ccacccccgg
     6001 ccgtgagttc gccttcgccg tcggcggtgc ccgcacccgc tggggctaca ccttcgagaa
     6061 gaccccgtcg ggcacccgcg tgaccgagtc gtgggaggtg ccggaggaga gtatgccgca
     6121 ctggcaggag cgtttcggcg agacgacgca gaaagagctc ggcatccgcg agcaggcggc
     6181 ccgcgaaggc atgcccgcga ccctcgccgc actcaaacgc gtagctgagg ccgcgcccgc
     6241 cgcgtgacgg gcacctcggc gacgagccgg tcacgcccgg cgagaatcgc gaagaccccc
     6301 tcggcgacac acacgcccac gagtacgaaa agcggcatcc gccagctgcc ggtgaggtcg
     6361 tgcagcgtgc cgagcacgat cgggccggag gcggcgatga ggtagccgac ggactgcgcc
     6421 atacccgaga gcgccgcggc ccgccggtgg tcgcgggtgc gcagcccgaa gagcgagagc
     6481 ccgacgacga tcgccccgcc ggccgagaag ccgatgaacg acacccatac gacggcgagc
     6541 gcgggcaggg cgagctggcc gagcaccccg atgagggaga acagggccag cacggcggcc
     6601 aggccgctct gattgcgcag ccgctgcaag agtgccccgg tagtgagcgt gcctgc
//
LOCUS       DASTNL010000021        11054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_388005, whole genome shotgun sequence.
ACCESSION   DASTNL010000021 DASTNL010000000
VERSION     DASTNL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388005"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFQ96_00570"
     CDS             <1..275
                     /locus_tag="VFQ96_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase"
                     /protein_id="HEU0256311.1"
                     /translation="LDVALFMAQVGHMGAPSDLKNLLHIVTYANAKIVGVDAGYGLNV
                     GDRADLVVLDAPDGPTGMLDRAVRSVVIKGGKVVARTEKHTELLPA"
     gene            616..1158
                     /locus_tag="VFQ96_00575"
     CDS             616..1158
                     /locus_tag="VFQ96_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEU0256312.1"
                     /translation="MLRKIPTEVVASIFAGITAFIGGNALNLPPWAIFIGWAGTYLVG
                     GPNIASMTKLWRTMPIGSTYALIIVLLDGWLGNGFGTSTLAKDAMLGLIILVVNTALM
                     FTGRIPFFALVPGMFFGFASFFATSFGGFGFAPGNPWAAWISVIAMNALGPVFAWLVE
                     WLTKAPKTAPDEKKKATVTD"
     gene            complement(1274..2026)
                     /locus_tag="VFQ96_00580"
     CDS             complement(1274..2026)
                     /locus_tag="VFQ96_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0256313.1"
                     /translation="MIILTALAVIAAGFAAGAVNTIVGSGSLITYPVMVFFGVPPVTA
                     NIANTIGLVPGSVAGAWGYRKRLKGMLRLLLRLGIVSLLGAIVGAVLLTQLPSTAFKL
                     AVPVLILAAALLVGLQPKIAAHTQGGGRTRLGQLTFWVFLASVYGGYFSAAQGVILLG
                     LLGVFLPVGLQEQNAVKNVLQAIVNIVAAVFFVILGHVAWFYVVCVAIGSVIGAPVGA
                     MLAQRIPARPFRIGIVIFGIAMAIVMAILALV"
     gene            complement(2023..3180)
                     /locus_tag="VFQ96_00585"
     CDS             complement(2023..3180)
                     /locus_tag="VFQ96_00585"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEU0256314.1"
                     /translation="MAITAVSSVALGLLRGAGVPLAVHSRFRSGVNLACGGFLAFAGP
                     GAASGACGIEVTAEDAALLATGDAWLWQGDGLHAVGSRAAASRGAGAALGDPARGGRR
                     AAVPLASDVRVYAPRVPRLHRLAEGAADVVEAARRAAGGVSWFDDGDGLTLGLPRLRA
                     ALATLFAAECPSGEGLTAAHPDDEGLVEHAGGGGLAGPAAGPSRTERSLRAALLRIVG
                     LGVGLTPSGDDAVIGALCVMAALGFLGAAERGAARAGAAAPERGAATGAGVFEPGLTR
                     DAAPERDSLSRSIERVLVDEAGGQRTTDVSASYLRLAVSGAFSPSLAAVVALLGRPHT
                     RRPAALGRPDPLRAAVTAELRYGATSGADSLVGVREACALLLAQPQPERVS"
     gene            complement(3199..4467)
                     /locus_tag="VFQ96_00590"
     CDS             complement(3199..4467)
                     /locus_tag="VFQ96_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEU0256315.1"
                     /translation="MKYASINEANEAVTQLITGGQPVLVDVVRAKDVIDVFQGGAKVL
                     LHAGPPIGFAEMPPPMQGSCVGAVLFEKWADDAESARAMLERGDVRFEPCHHHDAVGP
                     MGGITSGDMAMIVVENRAGGNRAYSTMNEGIGKVLRFGANDASVIARLEWMRDVLGPA
                     LSAAVKKSEGGIALMPIISRAITMGDEFHQRNIAASALFAKEVAPLLAQTDVPQSDLA
                     EVLRFLSDTDQFFLNLAMASCKAIMDAARTIEEGSVVTAMARNGKDFGIRVSGLGDEW
                     FTAPVNTPDGLYFTGYSSDQACPDMGDSAITETFGLGGMAMIAAPGVTRFVGAGGFGD
                     ALATSEEMAEIVTGHNPSLPIPTWDFQGACLGIDVRKVVQTSITPIINTGIASLEPGV
                     GQIGAGTVRAPLGCFVKALKALGEKYAVAK"
     gene            complement(4488..6173)
                     /gene="fdrA"
                     /locus_tag="VFQ96_00595"
     CDS             complement(4488..6173)
                     /gene="fdrA"
                     /locus_tag="VFQ96_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEU0256316.1"
                     /translation="MLTTVVKPNTYQDSVSLMLVSKKLTEMPDVIKVSVMMGTPANKD
                     ILRGTGFGSAALDSAQPGDMILAIDAEDNVDPQSVVHEAEEALANQAAGTPGSTLRTV
                     RSFERAFDVLPEANLGLLSIPGDYVAAEADRLLERGVNVMIFSDNVSLDDEIALKRKA
                     AAKGLLVMGPDCGTAMIGGAPLAFTNEVTRGDIGLVAASGTGAQEVMSAIDRLGGGVS
                     TVLGLGGRDLSHQVGGLMCLQALAALADDASTSTIVIVSKPPAPEVRQKVAEAAKNLG
                     KPVVIVFIGERPATARDGNVRYAYTLADAAALAVEIAGGGRPVFAPGQKAIRGLYTGG
                     TLANEAAMLIRDAFGLTDTDPSHRQGYVLRSGGHEIVDLGDDAYTQGRPHPMIDPSAR
                     IEKMPAVFDDPATAVVLLDCVIGYGASDDPAGALAPAIRDGIEHARAAGRDVAVVVSV
                     CGTRHDPQGYEEQRATLAEAGATVLPDNASAVRHALALLDEPAATSAGAAAPRPVGVA
                     GLLAGRPAVVNVGVRSFAQTLHDAEVSTVQYDWTPVAGGDAELASLLDALQNV"
     gene            complement(6288..6938)
                     /locus_tag="VFQ96_00600"
     CDS             complement(6288..6938)
                     /locus_tag="VFQ96_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0256317.1"
                     /translation="MSAPGEDESEAGRVTRLIRDEILDRVLEPGSPLVERELAARHGV
                     SRIPVRDALRTLVGEGLVTPRPRTWSIVRTFTPDEIADLHEVRAAIETLAFRLATQRH
                     TARGIEELRRVVSAELAAARQGDAIAARRAAADFHDTVVALAGNAVLEDLERSLRSRM
                     HWLLGRYDDLERVAHEHEALYAAIARRDEGAVGRLLERHMGTGKYLDGTARTMQVG"
     gene            complement(6995..7837)
                     /locus_tag="VFQ96_00605"
     CDS             complement(6995..7837)
                     /locus_tag="VFQ96_00605"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU0256318.1"
                     /translation="MTSTPHPAPADPTPPGKPGKPLSGLRVLVPRGGAWGDIVAAGLR
                     HAGASPVIAPMINFAPADDAPALQEAFARLADGAFDWLAITSATTVEVMTAWKAQVPS
                     ATRIAAVGETTAASLAAAGYHVDLIPTADNSAHGLLATWESATGGTVPLRILALRSQI
                     ANPLLTEGLRRIGHDVSAVVAYRTVGVAVPAQVVEDVAAGLVGAILVTSGSVAEQVQG
                     QLGPIPASTVVAAIGPHTAKDARAAGLRIDVVAEERSAESLIQAVVTALAPGGAGVEP
                     TDAG"
     gene            complement(7840..8811)
                     /gene="hemC"
                     /locus_tag="VFQ96_00610"
     CDS             complement(7840..8811)
                     /gene="hemC"
                     /locus_tag="VFQ96_00610"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU0256319.1"
                     /translation="MTVLRVGTRGSALALAQTDLVAVALAAAMHVEVEIVPVTTHGDV
                     SRQSLASMGGVGVFASALREALLAGDCDVVVHSLKDLPTEPVPGLAIGAIPKRADARD
                     VLVARDRLRLETLPPRARVGTGSPRRVAQLRAVRNDLDVVDIRGNVDSRLARLSAEDD
                     TRLDAVVLSAAGLTRLGRAEVIDVPLELSAWPTAPGQAALALEVREQDVSGTALAAGL
                     RAVNHATSRASAMAERGVLAGLGAGCSAPVGATAQLADGLFFLTATVYSLDGRASVTA
                     SHAATPDDRSAAALERAGREIAERVVAELLAEGAADIAPVGSQRGTE"
     gene            complement(8808..9962)
                     /locus_tag="VFQ96_00615"
     CDS             complement(8808..9962)
                     /locus_tag="VFQ96_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU0256320.1"
                     /translation="MTAGAFDALLLASFGGPEGQDDVLPFLRNVTAGRGIPEDRLEEV
                     AGHYRHFGGVSPINAQNRALKTALEAALASRGIDLPVYWGNRNWTPYFADAVRLAHEA
                     GHDRLLALVTSAYTSYSGVGQYREDFEKALADTGLGGVVTIDRIREFFDHPGFIAPLV
                     ESVRAAVTRFAEGDWPGSAGIHIVFVTHSIPVAAAEASGRGTGREFGTGGAYVAQHRA
                     VADLIAHRAAPGVPYSLAYQSRSGSPATPWLEPDVNDELERLAERGAVRSVVVVPIGF
                     ISDHMEVKWDLDNEAAETARDLDLRFARVATVGVQQPFIDGLVDLILERVNDVPEAER
                     RHETALGPWPDHPTVTGAGERRSVEGEPGKGATPAGGPESLAGGRHEVQV"
     gene            complement(9959..10687)
                     /gene="hemQ"
                     /locus_tag="VFQ96_00620"
     CDS             complement(9959..10687)
                     /gene="hemQ"
                     /locus_tag="VFQ96_00620"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEU0256321.1"
                     /translation="MTDPAAGTSPHPADSSSSDYEAPAIYTLWAVFRRDPANPATLDP
                     AATAAELHTALAAAEKSGVTVRGIYDVSGFKAESDLMFWLHGADPAALQAALRQLRRT
                     KAIAGLLPEWNYMAVHRAAEFNKHHIPAFVEGKAPKEWTCVYPFIRSHEWYLLRDGER
                     ARMLKEHGMVGHSYTGVLSNTVAAFALGDYEWVVPLESDELVQLTDMMRALRYVDARL
                     HVTLETPFFTGRRITPDEVAEVLS"
     gene            complement(10749..10988)
                     /locus_tag="VFQ96_00625"
     CDS             complement(10749..10988)
                     /locus_tag="VFQ96_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256322.1"
                     /translation="MTAENATAPAGRPTAVQKARADLVATLNEIEDRLNVPKRVWRSV
                     TGLRRGHPWLFAAAATAGAAALGAAGWLAVAVRRR"
BASE COUNT         1728 a         3881 c         3861 g         1584 t
ORIGIN      
        1 tcctcgacgt cgccctgttc atggcgcagg tcggccacat gggcgccccc agcgatctga
       61 agaacctgct gcacatcgtc acctacgcga acgccaagat cgtcggggtg gatgccggat
      121 acggcctgaa cgtcggcgac cgcgccgacc tcgtcgtcct cgacgcgccc gacgggccga
      181 ccggcatgct ggatcgcgcc gtgcgcagcg tcgtgatcaa gggcggcaag gtcgtggccc
      241 gcaccgagaa gcacaccgag ttgctgcccg cgtaagaacg ccacacttcc ggccgggtcg
      301 cgggcgagcg gcacacaccg cccgcaacca ccacgggccg cctcgaagac ccccacccag
      361 gaggacggcc aaggctcaac gttctgactc atcccgagac gtacgaagac cctcaccgcg
      421 gaaagcgacc cgatgtcgcg tctttgccgc cctccggcgg aggagccgac accacggagg
      481 gcggtcgaag gccgccggcc gagggcggcg gggcacaggc cccgcctcgg ccacctgtcc
      541 accggcccgc actctcgcag gcttctaccc caatcccgat ctccccctac atacacgaac
      601 acgcgaggag catccatgct gcgcaagatc ccgaccgagg tcgtcgcctc gatattcgcc
      661 ggcatcacgg cgttcatcgg cggcaacgcc ctgaacctgc cgccgtgggc catcttcatc
      721 ggctgggcgg gcacctatct cgtcggcggg ccgaacatcg cctcgatgac gaagctgtgg
      781 cgcaccatgc cgatcggctc gacgtacgcg ctcatcatcg tgcttctcga cggctggttg
      841 ggcaacgggt tcggcacctc gaccctggcg aaagacgcga tgctgggcct catcatcctc
      901 gtcgtcaaca cggcgctgat gttcaccgga cgcatcccgt tcttcgcgct cgtgcccggc
      961 atgttcttcg ggttcgcctc gttcttcgcc acctcgttcg gcggcttcgg cttcgcgccg
     1021 ggcaacccgt gggcagcgtg gatcagcgtg atcgcgatga acgcgctcgg ccccgtgttc
     1081 gcctggctgg tcgagtggct gacgaaggcc ccgaagacgg cacccgacga gaagaagaag
     1141 gcgacggtca ccgactagtc atctcacccg ccgccgcgcc acgtcgtaag gagacggcgg
     1201 tcaccgtcgg cctcgatcgc cgaactaccc gtgcgccccg cagcctcacc ggccgtgggg
     1261 cgcacgaggt ctgtcagacg agggcgagga tcgccatgac gatggccatg gcgatgccga
     1321 agatcacgat gccgatacgg aagggcctgg ccgggatgcg ctgcgccagc atcgcgccga
     1381 ccggcgcgcc aatgacggag cctatcgcga cgcagacgac gtagaaccac gccacatggc
     1441 ccaggatgac gaagaagacc gcggcgacga tgttcacgat cgcctgcaac acgttcttca
     1501 ccgcattctg ctcctgcagg ccgaccggca agaacacgcc aagcagcccg aggaggatga
     1561 ccccctgggc ggcgctgaag tagccgccgt aaacgctggc cagaaagacc cagaacgtca
     1621 gctgccccag gcgcgtgcga ccgccgcctt gcgtgtgagc cgcgatcttc ggctgcaggc
     1681 cgacgagcaa cgcggcggcg aggatcaaga cgggcacggc gagcttgaag gccgtggagg
     1741 gcagctgtgt cagaagcact gcgccgacga tggcccccag aagggagacg atgccgagcc
     1801 ggagaagaag ccgaagcatg cccttcaggc gcttgcggta gccccaggcg ccggcgaccg
     1861 agccgggcac gaggccgatc gtgttggcga tgttcgccgt gacgggcggc accccgaaga
     1921 acaccatgac cgggtaggtg atgagcgagc cggagccgac gatcgtgttg acggcgccgg
     1981 cggcgaagcc ggccgcgatc acggcaagcg cggtgagaat gatcacgaga ccctttcagg
     2041 ctggggttgg gcgagaagaa gcgcgcaggc ctcgcggacg ccgacgaggg agtcggcccc
     2101 cgaggtggcg ccgtaacgca gctcggccgt gacggcggcg cgaagaggat caggtcggcc
     2161 taaggcggcc ggccgcctgg tgtgcgggcg gccgagaaga gccacgacgg ccgcgagcga
     2221 cggcgaaaag gctccggata ccgccaggcg gaggtaggag gcggagacat cggttgtgcg
     2281 ctgcccgccc gcctcatcga cgaggacccg ctcgatcgaa cgcgagagcg agtctcgttc
     2341 cggcgcggcg tccctcgtca gaccaggttc gaagacgccg gcgcccgtcg ccgcgccgcg
     2401 ttcgggcgcg gcagccccag ccctcgccgc gccgcgctct gcggccccca gaaagccgag
     2461 cgccgccatg acgcacagcg cgccgatcac agcatcgtcg cccgacgggg tcaaccccac
     2521 gcccaagcca acgatccgca gaagcgcggc ccgtaacgac cgctcggtcc tcgacggccc
     2581 ggcggcgggc cccgcaaggc ccccgccacc cgcgtgctcg acgagacctt cgtcgtccgg
     2641 gtgcgcggcg gtcaggccct caccgctcgg gcactcggcg gcgaagaggg tggcaagtgc
     2701 cgcgcgcaac cgcggcagcc cgagcgtgag accgtcgccg tcatcgaacc agctgacccc
     2761 gcccgcggcc cgtctggcag cttcgacgac gtcggccgcg ccctcggcaa ggcgatgcag
     2821 ccgcggcacg cggggggcat acacgcgcac gtcggatgcc agcggcacgg ctgcccggcg
     2881 ccccccgcgc gccgggtcac cgagcgccgc accggcgccg cgcgaggccg cggcccgcga
     2941 gccgaccgcg tgcaagccgt cgccctgcca gagccacgcg tcgccggtcg cgagaagcgc
     3001 ggcgtcttcc gccgtcacct cgatgccgca cgcccccgag gctgcgcccg gccccgcaaa
     3061 ggcgaggaag cctccgcacg ccagattcac gccggagcgg aaccgcgaat gcaccgccag
     3121 cggcacgccc gcgccccgca gcaggccgag ggccacggaa ctcaccgccg tgatcgccat
     3181 gacgggcggg ttatcggctc acttggctac ggcgtacttc tcaccgagcg ccttcagcgc
     3241 cttcacaaag cagccaagag gtgctcgcac cgtgccggcg ccgatctgcc cgacgcccgg
     3301 ctcgagggag gcgatgcccg tgttgatgat cggcgtgatg gaggtctgca cgaccttgcg
     3361 cacgtcgatg ccgaggcagg ccccttggaa atcccaggtg gggatcggca gcgaggggtt
     3421 gtggcccgtg acgatctcgg ccatctcttc gctcgtggcc aacgcgtcgc cgaacccgcc
     3481 ggcgcccacg aaccgggtca cgccgggggc ggcgatcatg gccatgcccc ccagcccgaa
     3541 cgtctcggtg atggcgctgt cacccatgtc ggggcaggcc tggtctgagc tgtaaccggt
     3601 gaagtaaagg ccgtcgggcg tgttcaccgg agcggtgaac cattcgtcgc cgaggcccga
     3661 gacgcggatg ccgaagtctt tcccgttgcg ggccatcgcc gtgacgacgg agccctcttc
     3721 gatggtgcgc gccgcgtcca tgatcgcctt gcacgaggcc atcgccaggt tgaggaagaa
     3781 ctggtcggtg tcggagagga acctcaagac ctcggccagg tcggattgcg gcacgtcggt
     3841 ctgcgcaaga agaggcgcca cttctttggc gaagagcgcc gaggccgcga tgttgcgctg
     3901 atggaactcg tcgcccatcg tgatggcgcg cgaaatgatc ggcatcagcg cgatgccgcc
     3961 ctccgacttc ttcaccgcgg cggagagcgc cgggccgagc acatcacgca tccattcgag
     4021 gcgggcgatg acggaggcgt cgttcgcgcc gaagcgcagc accttgccga taccctcgtt
     4081 catcgtcgag taggcacggt tgccgccggc acggttctcg acgacgatca tcgccatgtc
     4141 gccgctcgtg atgccgccca tcgggccgac cgcgtcgtgg tggtggcagg gctcgaagcg
     4201 aacatcgccc cgctccagca tggcccgggc gctctcggcg tcgtcggccc acttctcgaa
     4261 gagcacggcg ccgacgcagg agccctgcat cggaggcggc atctcggcga agccgatggg
     4321 cgggcccgca tgcaggagaa ccttcgcccc accctggaag acgtcgatga catctttcgc
     4381 gcggaccacg tcgacgagca ctggctggcc gccggtgatc aactgggtga cggcctcgtt
     4441 ggcctcattg atggatgcgt atttcatagc gatgtttcct ccacctctca tacgttctgc
     4501 agcgcgtcga gcaggctcgc cagctcggcg tcgccccccg cgaccggcgt ccagtcgtac
     4561 tgcacggtgc tcacctcggc gtcgtgcaac gtctgggcga aggaacgcac gccgacgttg
     4621 acgaccgccg gtcggccggc aagaaggccc gcaacgccga ccggtcgcgg cgcggccgcc
     4681 ccagcggatg tcgcggcagg ctcgtcgagc aacgccaggg cgtgccgcac cgcggacgcg
     4741 ttgtcgggca gcactgtcgc gccagcctcg gcgagcgtgg cgcgctgctc ctcataacct
     4801 tgcgggtcgt gtcgcgtgcc gcagaccgag acgaccaccg cgacgtcgcg gccggctgcg
     4861 cgggcatgct cgatgccgtc gcgaatggcg ggggcgagcg cgccggccgg gtcgtcagac
     4921 gccccgtagc cgatgacgca gtcgagcaag acgaccgcgg tggccgggtc gtcgaagacg
     4981 gcgggcatct tctcgatgcg ggcgctgggg tcgatcatcg ggtgcggcct gccttgcgtg
     5041 taagcgtcgt cgccaaggtc gacgatctcg tgcccgcccg accgcaacac atagccctgc
     5101 cggtggctgg ggtcggtgtc ggtgaggccg aaggcatccc ggatcagcat ggccgcctca
     5161 tttgcgaggg tgccgcccgt gtagaggccg cgaatggcct tctgccccgg tgcgaagacc
     5221 gggcggccgc cgcccgcgat ctccacggcc agcgccgcgg cgtcggcgag cgtgtacgcg
     5281 tagcgcacgt tgccgtcacg ggccgtggca ggcctctcgc cgatgaagac gatgacgacg
     5341 ggcttgccga ggtttttcgc cgcctccgcc accttctgcc gaacctcggg agccggtggc
     5401 ttggagacga tcacgatcgt ggaggtcgag gcgtcgtccg cgagggcggc gagcgcctga
     5461 aggcacatga gcccgccgac ctggtggctg aggtcgcggc cgccgagccc gagaacggtg
     5521 ctcacgccgc cgccgagacg gtcgatggcc gacatgacct cttgtgcccc ggtgccggag
     5581 gcggcgacga ggccgatatc gccgcgcgtg acctcgttcg tgaaggcgag cggcgcgccg
     5641 ccgatcatcg cggtgccgca atccggcccc atgacaagaa ggcccttcgc ggcggccttg
     5701 cgcttcaagg cgatctcgtc gtcaaggctc acgttgtcgg agaagatcat gacgttgacg
     5761 ccgcgctcca gaaggcgatc cgcctccgcc gccacgtagt cgcccggaat ggagaggagg
     5821 ccgaggttcg cttcgggcag cacgtcgaac gcgcgctcga aggagcgcac ggtgcgcaac
     5881 gtggaacccg gcgtgccggc tgcctggttg gcgagcgcct cctccgcctc gtgcacgacc
     5941 gactgcgggt cgacgttgtc ttcggcatcg atggcgagaa tcatgtcgcc gggctgggcc
     6001 gagtcgagcg cggccgagcc gaacccggtg ccccgcaaga tgtctttgtt ggcgggggta
     6061 cccatcatga cggacacttt gatgacgtcg ggcatctcgg tcagcttctt gctgacgagc
     6121 atgaggctga cggagtcctg atacgtgttg ggcttgacga cagtggtgag catgaggcct
     6181 ccttgccgac ctcgtgtgcc gcgtggtgcg gccggcgcga tcggtacact tctcagtgta
     6241 aatggtatac cattccggtt ctcccggata ggccgggcgc cgttccgcta gccgacctgc
     6301 atggttcgtg ccgtgccgtc caggtacttt cccgtgccca tatgccgctc gaggagccgc
     6361 ccgacggcgc cctcgtcgcg gcgcgcgatc gccgcataaa gcgcctcatg ctcgtgcgcg
     6421 acccgctcca ggtcgtcgta tcggcccagc agccagtgca tccggctgcg cagcgagcgt
     6481 tccaggtctt cgagcaccgc gttgccggcc aacgcgacga cggtgtcgtg aaaatcggcc
     6541 gcggcgcggc gcgcggcaat cgcgtccccc tgccgagctg ccgccagctc cgccgagacc
     6601 acgcgtcgga gctcctcgat gccccgggcg gtgtgccgct gcgtcgcgag tcgaaaggcc
     6661 aaggtctcga tcgccgcacg cacctcgtgc aggtcggcga tctcgtcggg agtgaaggta
     6721 cgcacgatcg accaggtgcg tggcctgggg gtcacgagcc cctcgccgac aagggtgcgc
     6781 aaggcgtccc gcacgggtat gcggctcacc ccgtgccggg cggcgagttc ccgctcgacg
     6841 agcggactgc ccggctccag aacccggtcg agaatctcgt cgcggatgag cctcgttacc
     6901 cgccctgcct ccgattcgtc ttctcccggc gctgacacac cgacattttg ccatgcggcg
     6961 gcaggcgccg tctccgcggg agacggcggg gccctcagcc ggcgtcggtg ggctcgaccc
     7021 ccgcgccgcc cggggcgagc gccgtgacga cggcctggat gagcgactcc gcagaacgct
     7081 cctccgccac cacgtcgatg cgcagcccgg ctgcccgggc gtccttggcg gtgtgcgggc
     7141 cgatcgccgc gaccaccgtc gaggcgggga tcgggcccaa ctggccctgc acctgttcgg
     7201 cgacgctgcc agaggtgacg aggatggcac ccaccaggcc cgccgccacg tcctccacca
     7261 cctgggcggg cacggcgact cccaccgtgc gataggcgac gaccgccgag acgtcgtggc
     7321 cgatgcgacg cagaccctcg gtgagcaacg ggttcgcgat ctggctgcgc agcgccaaga
     7381 tcctcaacgg cacggtgccg cccgtggcgg attcccaggt ggccaagagg ccgtgcgccg
     7441 agttgtcggc ggtggggatg aggtcgacgt gatagccggc cgctgccagg gaggcggccg
     7501 tcgtctcgcc gacggccgcg atgcgcgtgg cggagggcac ctgggctttc caggcggtca
     7561 tcacctcgac ggtcgttgcg ctcgtgatgg caagccagtc gaatgcgccg tccgcgagcc
     7621 tcgcgaaggc ctcttgcagc gccggggcgt cgtcagccgg cgcgaagttg atcatcgggg
     7681 cgatgaccgg gctggcgccc gcgtgacgca gccccgcggc gacgatgtcg ccccacgcgc
     7741 cgccgcgcgg caccaacact cgcaggcccg acaacggctt gcccggcttg ccgggcggtg
     7801 tcgggtccgc cggcgcggga tgcggggtgc tggtcatctc tactctgtgc ctcgttgaga
     7861 tcctaccgga gcgatgtcgg ccgccccctc ggccagcagt tctgcaacga cgcgctcggc
     7921 gatctctcgc cccgcacgtt ccaacgcggc ggccgaccgg tcgtccggtg tggccgcgtg
     7981 agaggccgtg accgaggccc ggccgtccag gctgtagacg gtggcagtca agaagaagag
     8041 gccgtcggcc agctgcgcgg tggcgccgac cggcgccgag catcccgcac cgaggccggc
     8101 gaggacgcca cgctcggcca tcgccgaggc ccggctcgtc gcatggttga cggcccgcaa
     8161 accggccgcg agggcggtgc ccgagacatc ctgctctcgc acctcgagcg ccagcgcggc
     8221 ctggccgggc gcggtgggcc aggcgctcaa ctccagcggc acgtcgatca cctcggccct
     8281 gccgagcctc gtcaggcctg cggctgagag cacaacggcg tcgaggcggg tgtcgtcttc
     8341 ggccgacagg cgcgcgaggc gtgagtcgac gttgccgcgg atgtcgacca cgtcgaggtc
     8401 gttccgcacg gcacgcagct gggcgacgcg gcggggagac cctgtgccca cccgggcccg
     8461 cgggggcaga gtctccaaac gcagcctgtc gcgggcgacg agcacgtccc gagcgtcggc
     8521 acgtttcggg atcgcgccga tggcgagccc cggcacgggt tcggtcggca gatctttcag
     8581 cgaatggacg accacgtcgc agtcgccggc gagaagcgcc tcgcgcagcg cgctggcgaa
     8641 gacgccgacc cctcccatgc tggccagcga ctgccgggag acgtcgccgt gggtcgtcac
     8701 cggcacgatc tccacctcga catgcatcgc ggcggccagc gcgacggcga ccaggtcggt
     8761 ctgggccaga gccagcgcgc tgccgcgggt tcccacccgc agcacggtca tacctgtacc
     8821 tcgtgccgtc cgccggcgag gctttcgggg ccgcccgcag gcgtcgcgcc cttgccaggc
     8881 tccccctcca ccgatcgccg ctcaccggcg ccggtgacgg tgggatggtc gggccagggg
     8941 ccgagggccg tctcgtgccg gcgttctgcc tcgggcacat cgttcacgcg ctccaagatc
     9001 aagtcgacga gcccgtcgat gaacggctgt tgaacgccga cggtcgccac ccgggcgaag
     9061 cgcaggtcaa ggtcgcgcgc ggtctctgcc gcctcgttgt cgagatccca cttcacctcc
     9121 atatggtcgc tgatgaagcc gatcggcacg acgacgacgg aacgcacggc gccgcgctca
     9181 gccaggcgct cgagctcgtc gttcacgtcg ggctcgagcc acggcgtcgc cgggctgccg
     9241 ctgcgtgatt ggtaggccag cgaatagggc acgcccggtg cggcacggtg cgcgatgagg
     9301 tcggcgacgg cgcgatgctg cgcgacgtag gcgccgcccg tgccgaactc ccgcccggtt
     9361 ccccggccgg aggcctccgc cgcggcgacc gggatggaat gcgtcacgaa gacgatgtgg
     9421 atgccggcgg aacccggcca gtcaccctcc gcgaacctgg tcacggccgc ccgcacactc
     9481 tcgaccaagg gggcgatgaa gcccggatgg tcgaagaact cgcggatccg gtcgatcgtg
     9541 acaacgcctc ccagccccgt gtcggcgagc gccttctcaa agtcttcgcg gtactgcccc
     9601 acgcccgaat acgaggtgta cgcgctcgtc acgagggcga gcaaccggtc gtggccggcc
     9661 tcgtgggcaa gacgcaccgc gtcggcgaaa tacggggtcc agttgcggtt gccccagtag
     9721 acgggcaggt cgatgccgcg cgaggcaagg gcggcctcga gggccgtttt gagcgcacgg
     9781 ttctgagcgt tgatgggcga gacaccgccg aagtggcggt agtgccccgc aacctcctcg
     9841 agccggtcct cggggatgcc gcggccggcc gtcacgttac gcaagaacgg cagcacatcg
     9901 tcttgcccct cgggcccgcc gaagctggcc agcaggagcg catcgaaagc gcctgccgtc
     9961 aagacaagac ctcggccacc tcgtcggggg tgatgcgccg gccggtgaag aacggcgtct
    10021 ccagggtgac gtgcagccgg gcgtcgacgt agcgcaacgc ccgcatcatg tcggtcaact
    10081 gcaccagctc gtcggattcg agcggcacga cccattcgta gtcgcccagc gcgaacgcgg
    10141 cgacggtgtt cgagagcacg ccggtgtaag aatggcccac catgccgtgt tccttgagca
    10201 tgcgggcacg ctcgccgtca cggagcagat accattcgtg gctgcggatg aacgggtaga
    10261 cgcacgtcca ttctttggga gccttgccct ccacgaaggc cgggatgtgg tgcttgttga
    10321 actcggcggc gcggtgcacg gccatgtagt tccattccgg caggaggccg gcgatcgcct
    10381 tggtgcgacg caactgtcgc agggccgcct gcaacgcggc cggatcggcg ccgtgcagcc
    10441 agaacatgag gtctgattcg gccttgaagc ccgagacgtc gtagatgcca cgcaccgtga
    10501 cccccgactt ctcggcggcg gcgagagcgg tgtgcagctc ggcagcggtc gcggccgggt
    10561 cgagtgtcgc cgggttcgcc gggtcgcgtc ggaacacggc ccacagagtg taaatcgccg
    10621 gcgcctcata gtcggaggac gaggagtcgg cagggtgggg ggaggttccg gcggcggggt
    10681 ctgtcatacc tgccatcatt actcggatcg agcccgaggt ccaaaccttc tcagaggcat
    10741 atgtgggctc agcggcgacg cacggcgacg gccagccatc cggctgctcc gagtgcggca
    10801 gccccggccg tcgcggcggc ggcgaaaagc cacggatggc cgcggcgaag gcccgtgaca
    10861 gagcgccaga cccgcttggg aacgttcagt cgatcttcga tctcgttcaa ggtcgcgacg
    10921 agatcggcgc gggccttttg cacggccgtg ggccggcctg caggggccgt tgcgttctca
    10981 gcggtcatag cgacccatcc ccctcaacgc gtcgatgtcc tcgtgcaggc tcgccgcggc
    11041 cttcttggcc gtcg
//
LOCUS       DASTNL010000022        13077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_945129, whole genome shotgun sequence.
ACCESSION   DASTNL010000022 DASTNL010000000
VERSION     DASTNL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945129"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFQ96_00630"
     CDS             complement(<1..291)
                     /locus_tag="VFQ96_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU0256323.1"
                     /translation="MSDTRLVHTTIEVRDAVHVAHAAGRRVSFVPTMGALHEGHLALL
                     HAAREYGDFVVVSIFVNPLQFGPGEDYARYPRTLGDDMAALAGHADLVFAPGV"
     gene            complement(288..1118)
                     /locus_tag="VFQ96_00635"
     CDS             complement(288..1118)
                     /locus_tag="VFQ96_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEU0256324.1"
                     /translation="MDGLVSAGRAGRLGVGVIGAGHVGPVLGAALAGAGHALVGVAAV
                     SAESRDRAGALLPGVPLLDVPVLVERSELVVVAVPDDELAGLVAGLAGVGAWQAGQLV
                     LHTSARHGIGVLAPARERGAIPLAVHPALTFTGTSIDVGRLSGSYFAVTAPAPVLPIA
                     QALVVEMGGEPVVIDEADRAAYAEAIEVATAFPRRVIARSAGLLSGIGVPNPGGFLAS
                     LVQSTVDEALRAFVVTDEPLPSPEQLFGAGAPGPGPAPSWPAEGSPRGRKPPQRRSET
                     "
     gene            complement(1103..2650)
                     /locus_tag="VFQ96_00640"
     CDS             complement(1103..2650)
                     /locus_tag="VFQ96_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU0256325.1"
                     /translation="MPGPAVVEADGAWHRLHPATPLLRGGVAVIAVIGIVMANLRERI
                     ADLAMRFAGVRDVPDDSGDPVNAIVRHGLIGWALLIVLAVLLVAVGLFYLSWRMHTFR
                     VGDDEVEVRSGVVSRRYRNARLDRIQGVNLSKPLFARLFGTAKLEVSVAGQDANVELA
                     YLALGTATGLRREILRRASGTRRAAAQARAAGGDLIGRRLDDFLSPHYDPDAPAPTSA
                     VVIPPLRLFGAIVISGPTLVLAAIVAGVVVAAFRGHYLLLVVLAPSILAWGGMYIRRF
                     VRSMNYSIVATAEGIRVGFGLFSTSNETIPPGRIHALEVVQPLMWRPFGWWQIRIDTA
                     GRLKDKGAAGQHNTHILPVGDPYAVEKVLGVVVPALADPGHVALITAGMSETGAEAAA
                     PAGQTGPGVRFTMAPRRAAWLRPFSWRRVGFILADDAVLLRTGAIWRRLTIVPLARLQ
                     SMELRQGPVRRRLGLVHARLHTVDGPVHAGLPVIDAAEGIRLFHTVARAAVIAAAADH
                     GERWTAS"
     gene            complement(2779..3261)
                     /locus_tag="VFQ96_00645"
     CDS             complement(2779..3261)
                     /locus_tag="VFQ96_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU0256326.1"
                     /translation="MAAHLDMGADWQRVSARYVAVIIVGNIVWGVVVAAAVLVLWLRF
                     GVSWAWLLLVADAIATVTMLLIAPRRARSIGYQLRDDDLLFCRGIMFQRYVAVPYGRM
                     QLIDINRGPVGRLLHLADLKFVTAAATTGVGIPGLHDDTAAGLRDRLMALAETRRTGL
                     "
     gene            3399..3869
                     /locus_tag="VFQ96_00650"
     CDS             3399..3869
                     /locus_tag="VFQ96_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0256327.1"
                     /translation="MSRQNRPYIDKTFPEAYQAAVRAAREARQAAADAGLDRAVVELV
                     NVRVSQLNGCLTCLSTHTPAARSAGVPQEKLDLLPAWREGAAAFTGVEKAALRIAESL
                     TVIDRAEDREALGAAAAEYLTTEQIAAVEWVTILINAFNRISIASGHPALPARA"
     gene            complement(3930..4655)
                     /locus_tag="VFQ96_00655"
     CDS             complement(3930..4655)
                     /locus_tag="VFQ96_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU0256328.1"
                     /translation="MSGTTADGRPVGVVSPGRERSGGEVRRRARGRDRVERLFGTYLP
                     GHSVIHRLPAGVKLLALAAVGITAAAVPRPEVLAAAGVVTFAAFLLAGLVGHLARQLA
                     RLAWLILAVAAGQLLFALPVPLVVANTARMTIVIVLASLVTVTTPASQVLAALERGLQ
                     PLRRFGVDPPRVALTLSLTLRTVPVLARQAAGIRDAQWARSGRYSVTAFVVPLLVAAL
                     RQADRLADALAARGLDKGPRPPR"
     gene            complement(4636..5343)
                     /locus_tag="VFQ96_00660"
     CDS             complement(4636..5343)
                     /locus_tag="VFQ96_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256329.1"
                     /translation="MTPCHAGPQEEGIRFEGVSVERGEARILDDVTVTLRARRTAIIG
                     RNGSGKSTFVRLVNGLVEPDAGSVAVDGLDSVRERRRLRRMVGFLFTDADSQIVMPTV
                     AEDVAFSLRGRGLDRAEIQRRVTEILARFGLAERAETPAHELSGGQQKLLALCSILVA
                     EPRIVVADEPTALLDAVNTRRITGVLRRLSQRLIVVTHDLGFAAGCDEALLFHESRLV
                     AHGAPADVIAQYERDYC"
     gene            complement(5340..5987)
                     /locus_tag="VFQ96_00665"
     CDS             complement(5340..5987)
                     /locus_tag="VFQ96_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU0256330.1"
                     /translation="MRSGSRSTTADMARIATFAALIAVMGLVPPLPLFGNVTPITLQT
                     LGVMLAGAILGGVPGALSVVVFLLLVAAGMPFLAGGQGGLGAFFTPSAGFLIGWVPSA
                     AVVGWLARPHGGRPAWWRVALGTVVGGILVVYAIGIPVQALVTGIPLAKTALLSLMFV
                     PGDLAKAVVTVLLVTALARAYPRAFRRSGRGAGRRPADARPANDGADRSVVGGRS"
     gene            complement(6086..6565)
                     /locus_tag="VFQ96_00670"
     CDS             complement(6086..6565)
                     /locus_tag="VFQ96_00670"
                     /inference="COORDINATES: protein motif:HMM:NF022819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HEU0256331.1"
                     /translation="MRRSSPSVLVGVALAGLVGGFLLEVALVSRGMPMLVPPRSLPIA
                     LLLIGAIALGLAIPIHRAVRGTRKSRIDPFQAVRVAVLAKASSLLGAAATGIAVGMLV
                     YAFTRPLLPQGSLVLLDVAFIVAAALLLAAGLVAEYLCTLPPPEDGEAADAARPDRG"
     gene            complement(6579..7610)
                     /gene="folK"
                     /locus_tag="VFQ96_00675"
     CDS             complement(6579..7610)
                     /gene="folK"
                     /locus_tag="VFQ96_00675"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU0256332.1"
                     /translation="MTTTTPQPADRPTAAARWADHTVPGDHITLTGLRAFAHHGVFQH
                     EKNDGQEFVADVTVWFDIHAAAAGDDVAATVDYGRLAGGVVAAIERDPVDLIETVAER
                     IAAVALGFAAVRLARVTLHKPHAPITVPFDDVAVTIERGRAVQGRDRGRDSAREAGPR
                     GAGGTHAVVLALGANLGEREENLRGAVADLAASSGIEVTAVSALYATPALTLHGVDRN
                     APAYLNAVVLARTRLHPYALLEATSAIEDAHGRVRTTRWGARTLDIDIIDYDGRRLDD
                     PTLTLPHPRAGERAFVLVPWLDVDPGALLVGRGPVADLVGSVDDEATPHADRHWADGA
                     WPAPSARPA"
     gene            complement(7607..8395)
                     /gene="folP"
                     /locus_tag="VFQ96_00680"
     CDS             complement(7607..8395)
                     /gene="folP"
                     /locus_tag="VFQ96_00680"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0256333.1"
                     /translation="MGVLNVTPDSFSDGGRYATTDAAVAHAVEMVRQGADLVDVGGES
                     TRPGAVRVDQVEEQRRAIPVIRELCARGIKVSIDTMRSQTANAAVRAGATVVNDVSGG
                     LADPAMAGVVAQSGLPYVVMHWRGHSATMNARAAYRDAAKEVREELLARVDALAAGGM
                     PYEQMILDPGLGFAKDARHNWQVLAGLDGLIATGFPILVGASRKRFVGALLPEGAPME
                     ERDAPTAVISALAARAGAWAVRVHDPASTRRALDVVEAWQAGAR"
     gene            complement(8491..10506)
                     /gene="ftsH"
                     /locus_tag="VFQ96_00685"
     CDS             complement(8491..10506)
                     /gene="ftsH"
                     /locus_tag="VFQ96_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0256334.1"
                     /translation="MNAKKIFHGPIFYIVLAVIAVWIGSSLITMPGFKSIPTYEGMKL
                     IQSGQVQSAKIIGGSDNTVDLTLSKAYKNAGTKVQFQFLDARGPAVIKAIDAAQPPKG
                     YNDQVPQPNWFLSALGFLLPVLAIGVIFWLMLSSMQGGGSKVMQFGKAKAKLVSKETP
                     KVTFDDVAGADEAVEELQEIKEFLKEPAKFQAVGARIPKGVLLYGPPGTGKTLLARAV
                     AGEAGVPFYSISGSDFVEMFVGVGASRVRDLFQQAKENAPAIIFVDEIDAVGRHRGAG
                     MGGGHDEREQTLNQLLVEMDGFDVKTNVILIAATNRPDILDPALLRPGRFDRQVGVDA
                     PDMKGRRKILEVHSKGKPLSKNVELDVLARKTPGFTGADLANVLNEAALLTARSDAQL
                     IDNRALDEAVDRVVAGPQRRTRVMKDKEKLITAYHEGGHALAAAAMRHTDPVTKITIL
                     PRGRALGYTMVLPIEDKYSVTREELLDQMAYAMGGRVAEEVVFHDPTTGASNDIEKAT
                     GIARKMVTEYGMSADIGSVKLGQPSGEVFLGHNMGQERDYSENVAEKVDQEVRALIEK
                     AHDEAWQVINANRDILDRLAGELIEKETLDHIRIEEIFADVVKLPERPLWLSSQNRPV
                     SDRPPITIPEPPHPEAGLSDDDTGAKPPAPAAKPAKLPFHKPRPATA"
     gene            complement(10642..11196)
                     /gene="hpt"
                     /locus_tag="VFQ96_00690"
     CDS             complement(10642..11196)
                     /gene="hpt"
                     /locus_tag="VFQ96_00690"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU0256335.1"
                     /translation="MEPTDVTQDLTTVLVTEQQIRARLADIARQIEHDYEGKDVLLVG
                     VLKGAVMVMADLSRELRIPVTMDWMAVSSYGSGTHSSGVVRILKDLDADLSGRHVLIV
                     EDIIDSGLTLSWLSSNLRSRGPASVEICALLRKPAAARVDIEVRYVGFDIPNDFVVGY
                     GLDYDERYRNLRGVGVLAPRVYGQ"
     gene            complement(11248..12399)
                     /gene="tilS"
                     /locus_tag="VFQ96_00695"
     CDS             complement(11248..12399)
                     /gene="tilS"
                     /locus_tag="VFQ96_00695"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU0256336.1"
                     /translation="MAPTDSAPVAAEGLGTGVSGAPGPSAGPVRRTRLTPAVADVRRA
                     VRQGLGAFLDPADDGVHFPRCARPDRVAPAPSSLAAPDVPLVLVALSGGPDSLALAAA
                     VAFEAPRMAARAGAVIVDHGLQEGSAQVAAQAAATARSLGLAPVLVRCVAVGGDSEAA
                     ARQARYAALDEAVRQTGARAVLLAHTLDDQAETVLLGLARGSGARSLAGMRPVAGHYL
                     RPLLQVPRVTTHRACDDLGLHPWHDPMNADARYARPRVRDVVLPTLEAQLGPGVAAAL
                     ARTAGQLAEDADALDQIAQELLQDLCEPAEAGLAIPVGVLAANPPALRHRVIRAAVRA
                     EFGTSLERVHVLAVAGLITNWHGQAALDLPGVRVVRQGGRLVFSAPGRA"
     gene            12507..13001
                     /gene="ppa"
                     /locus_tag="VFQ96_00700"
     CDS             12507..13001
                     /gene="ppa"
                     /locus_tag="VFQ96_00700"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU0256337.1"
                     /translation="MAEYDAIIEIPRGSRNKYEVDHETGKVMLDRVLFTGFVYPADYG
                     FIEKTLGLDGDPLDVLVLLQYPLFPGVGVRVRPVGVLKMSDEAGNDAKIIGVPYKDPR
                     WAGIRDVDDVPEGTRQEIEHFFTHYKDLEPQKWVRVGRWGNAEEARTVIAEAIERAKS
                     AATA"
BASE COUNT         1998 a         4583 c         4754 g         1742 t
ORIGIN      
        1 gacgccgggc gcgaagacca ggtcggcatg gcctgcgagc gcggccatgt cgtcgcccag
       61 cgtgcggggg tagcgcgcgt agtcttcgcc ggggccgaac tgcagcggat tcacgaagat
      121 ggagaccacg acgaagtcgc cgtattcgcg cgcggcgtga aggagcgcga ggtggccctc
      181 gtggagggcg cccatggtcg gcacgaagct gacgcgtctg ccggccgcgt gggcgacgtg
      241 gacggcgtcg cgcacctcga tggtggtgtg gacgaggcgg gtgtcgctca tgtctcgctc
      301 cttctctgag gaggctttcg gcctcgtggc gaaccctcgg ccggccacga cggggccggc
      361 ccggggcccg gcgctcccgc cccgaagagc tgctccgggc tgggcagggg ctcgtcggtg
      421 acgacgaagg cgcgcagcgc ctcatctacg gtcgactgca cgagggaggc aaggaagccg
      481 ccggggttcg gcacgccgat gccgctgagg agccccgccg aacgggcgat gacccgccgg
      541 gggaacgcgg tcgcgacctc gatggcctcc gcgtaggcgg cgcgatccgc ctcgtcgatg
      601 acgaccggct cgccgcccat ctcgacgacg agcgcctggg cgatgggcag caccggggcg
      661 ggggcggtca ccgcgaagta gctgcccgac agacggccga cgtcgatgct cgtgccggtg
      721 aaggtcaagg ccgggtgcac ggccagcggg atggcgccgc gctcgcgtgc cggggcgagc
      781 acgccgatgc cgtgccgtgc ggaggtgtgc agcaccagct gcccggcctg ccacgcgccc
      841 accccggcga gtccggcgac gaggcccgcc agctcgtcgt cgggaacggc cacgacgacg
      901 agctcgctgc gctcgacgag gacgggcacg tcgagaagcg gcacgccggg cagtagcgcc
      961 cccgctcggt cacggctctc ggccgacacg gcggcgacgc cgaccagggc gtgcccggcg
     1021 ccggccaacg ccgcgccgag aacgggcccg acgtggccgg cgccgatgac gcccacgccg
     1081 agtcggccgg cccgcccggc actcacgagg ccgtccatcg ctcgccgtgg tcggccgcgg
     1141 ctgcgatgac ggcggcgcgg gccacggtgt ggaagagccg gatgccctcg gccgcatcga
     1201 tgaccggcag gccggcatga accgggccgt ccaccgtgtg cagccgggca tgcaccagcc
     1261 cgaggcgccg ccgtacgggg ccctgccgca gctccatgct ctgcagccga gcaaggggaa
     1321 cgatcgtcag ccggcgccag atcgcgccgg tgcgcaggag caccgcgtcg tcggccagga
     1381 tgaacccgac gcgccgccac gagaagggcc gcagccaggc ggcacggcgc ggcgccatcg
     1441 tgaaccggac cccgggcccc gtctgcccgg ccggggccgc ggcctcggcg cccgtctcgc
     1501 tcatgcccgc cgtgatgagg gcgacgtggc cgggatccgc cagggcgggc acgacgactc
     1561 ccagcacctt ttcgacggcg taggggtcgc ccaccggcag gatgtgcgtg ttgtgctggc
     1621 ccgcggcgcc cttgtctttc agcctgccgg ccgtgtcgat gcggatctgc caccagccga
     1681 acggccgcca catgagtggc tgcacgacct ccagcgcgtg gatgcgcccg ggcgggatcg
     1741 tctcgttgct cgtggagaag agcccgaatc cgacgcggat cccctccgcc gtggcgacga
     1801 tgctgtagtt catcgaacgc acgaagcggc gaatgtacat gccgccccac gcgaggatcg
     1861 acggcgccaa gacgacgagc agcaggtagt ggccgcggaa ggccgcgacg acgacgcccg
     1921 cgacgatcgc cgcgagcacg agggtaggcc cgctgatgac gatcgccccg aagagcctca
     1981 gcggcgggat cacgacggcg gaggtgggcg ccggcgcgtc cgggtcgtaa tgcggcgaaa
     2041 gaaagtcgtc gagccgccgg ccgatgaggt cgccgcccgc ggcgcgcgcc tgggcggccg
     2101 cgcggcgggt gccggaggcg cggcgcagga tctcgcggcg cagcccggtc gccgtgccca
     2161 gcgcgagata ggccagctcg acgttggcat cttggcccgc gacgctgacc tcgagcttgg
     2221 cggtgccgaa gagccgggcg aagagcggct tggagaggtt cacgccctgg atgcggtcga
     2281 gtcgggcatt acggtagcgc cggctgacga cgccgctacg cacctcgacc tcgtcgtcgc
     2341 cgacgcgaaa ggtgtgcatc cgccacgaca ggtagaagag cccgacggcg acaaggagca
     2401 cggcaagcac gatgagaagt gcccagccga tgagcccatg ccgcacgatc gcgttgaccg
     2461 ggtcgccgga gtcgtcgggc acgtcgcgca cgccggcgaa acgcatggcg aggtcggcga
     2521 tgcgttcgcg caggttcgcc atgacgatgc cgatgacggc gatcaccgcc accccgccgc
     2581 gaaggagcgg cgtggccggg tgcagccggt gccaggcacc gtcggcctcg accacggccg
     2641 ggcccggcac ccccttcgcc agtgtgccgg gcgccgggtc gggcgacccc ggttccgacg
     2701 gcgcgggtcg gaaccttcca ggcgggccct gcggcggcgg gagcctcccg ccggcgggtg
     2761 ccgggcctcg ggcgcccgtc acagccccgt ccttcgggtc tcggcgagag ccatgagccg
     2821 gtcgcgcaac cccgccgccg tgtcgtcgtg cagcccgggg atgccgaccc ccgtcgtcgc
     2881 ggccgccgtc acgaacttga ggtcggccag gtgcagcagg cgacccaccg ggccgcgatt
     2941 gatgtcgatc agctgcatac ggccgtaggg caccgccacg taacgctgga acatgatgcc
     3001 gcggcagaag agcagatcgt cgtcgcgcag ctgatacccg atcgaacggg ccctgcgcgg
     3061 cgcgatgagg agcatcgtca cggtggcgat ggcgtcggcc acgagcagca accacgccca
     3121 cgagacgccg aaccgcagcc acaggacgag cacggccgcg gcgacgacga cgccccagac
     3181 gatgttgccg acgatgatga cggcgacata gcgtgcggag acccgctgcc agtcggcgcc
     3241 catgtcgaga tgtgccgcca cgccgctccc gcccgctcgc ccgcggccgg gccggccgcc
     3301 cggcctcacc ctctccatgc tacgggaccg gccgcgggcc gacatggccg ccgaggcctc
     3361 caaggcctac cgtgggaggc accgtgcgaa ggaggagcat gtctcgtcag aaccggccct
     3421 acatcgacaa gacttttccg gaggcgtacc aggcggccgt gcgcgccgcc cgcgaggccc
     3481 ggcaggcggc cgccgacgcg ggcctcgacc gggcggtcgt cgagctcgtg aacgtgcgcg
     3541 tctcgcagct gaacggctgt ctcacctgcc tcagcacaca cacgccagcg gcccgttccg
     3601 ccggcgtgcc ccaggagaag ctcgacttgc tgcccgcctg gcgggaaggg gccgcggcgt
     3661 tcaccggcgt cgagaaggcc gcgctgcgca tcgcggaatc gctcaccgtc atcgaccgcg
     3721 ccgaagaccg ggaagcactg ggggcggcgg ccgcagagta cctgacgacg gagcagatcg
     3781 cggccgtcga atgggtcacg attctgatca acgccttcaa ccgcatctcg atcgcgagcg
     3841 gccacccggc gttgcccgcg cgggcctgac cgaggcgcac cagcctcgcc gcgccgggcc
     3901 ccacagccgg gcgtatgccg gcgcgagcgt cagcggggcg gccgggggcc cttgtcgagg
     3961 ccgcgcgccg cgagggcgtc ggcgagccgg tccgcctggc gaagggcggc gacgagcagc
     4021 ggcacgacga acgccgtgac gctgtagcgg ccggaccggg cccactgcgc gtcgcgaatc
     4081 ccggccgcct ggcgggcgag cacgggcacg gtgcgtaacg tcagggagag ggtcagggcg
     4141 acacgaggcg ggtcgacacc gaaccgtcgc aacggctgca gccctcgctc gagcgcggca
     4201 agcacctggg aggccggcgt cgtcacggtg acgagcgagg ccagaacgat gacgatcgtc
     4261 atgcgcgccg tgttcgcgac gacgagcgga acgggcaggg cgaagagcag ctggccggcg
     4321 gcgacggcga ggatgagcca ggccagccgg gcgagctggc gcgcgaggtg cccgacgagg
     4381 ccggcgagaa gaaacgcggc gaacgtcacg accccggccg ccgcgagcac ttccgggcgg
     4441 ggcaccgccg cggccgtgat gcccacggcg gccagcgcca gaagcttcac gccggccggc
     4501 aggcggtgga tcacgctgtg ccccggaagg tacgttccga agaggcgctc cacccggtcg
     4561 cggccgcgcg cgcgccgcct cacctcgccc cccgaacgct cgcggccggg cgagacgacg
     4621 ccgacggggc ggccatcagc agtagtcccg ctcatactgg gcgatcacgt cggccggcgc
     4681 cccgtgggcg acgagccgcg actcgtggaa gagcagcgcc tcgtcacacc cggcggcgaa
     4741 gccgaggtcg tgggtgacga cgatgagccg ttgcgagagc ctgcgcaaga cgccggtgat
     4801 gcgccgggtg ttgacggcgt cgagcagcgc ggtcggctcg tcggcgacga cgatccgcgg
     4861 ttcggcgacg agtatcgagc acagggcgag cagcttctgc tgccctcccg acagctcgtg
     4921 agcgggggtc tcggcgcgct cggcgagccc gaaccgggcg aggatctcgg tgacgcgccg
     4981 ctggatctcg gcgcggtcga ggccccggcc gcgcagtgag aaggcgacgt cttcggcgac
     5041 ggtgggcatg acgatctggc tgtcggcgtc ggtgaagaga aacccgacca tgcggcgcag
     5101 tcgcctgcgc tcacgcacgc tgtcaagccc gtcgaccgcg acgctgccgg catcgggctc
     5161 gacgagcccg ttcacgaggc gcacgaacgt cgacttaccg gagccgttgc gcccgatgat
     5221 cgccgtgcgc ctagcccgca gcgtgacggt gacgtcgtcg aggatgcggg cctcgccgcg
     5281 ctccacgctg acgccctcga accgaatgcc ctcctcctgc ggccccgcgt gacagggggt
     5341 cacgagcgcc cgccgacgac gctccgatcc gcgccgtcgt tcgccggccg ggcatcggcc
     5401 gggcgccggc ccgcaccccg gcccgatcgg cggaacgccc tcgggtaggc gcgcgcaagc
     5461 gcggtgacga gaagcacggt cacgaccgcc ttggcgaggt cgccgggcac gaacataagg
     5521 ctcaggagcg cggtcttcgc aagcgggatg cccgtgacga gcgcctgcac gggaatgccg
     5581 atcgcataga ccacgaggat gccgccgacg acggtgccca gcgcgacgcg ccaccacgca
     5641 ggccgcccgc cgtgaggccg cgcgagccag ccgacgaccg cggcgctcgg cacccagccg
     5701 atcaagaacc cggcgctcgg ggtgaagaat gccccgagcc cgccctgtcc gccggccaag
     5761 aagggcatcc cggcggcgac gaggaggagg aacaccacga cggagagggc cccggggaca
     5821 cccccgagga tcgccccggc caacatcacg ccgagcgtct gcaacgtgat cggggtgacg
     5881 ttgccgaaga gggggagcgg cggcaccagg cccatgacgg cgatgagggc cgcgaaggtg
     5941 gcgatgcggg ccatgtcggc cgtcgtcgaa cgagatccgc ttctcatagc ttccaggttc
     6001 ccacgcccgt gagggtcacc ttcggtgtgg atggtcgatg aatcgcgccg ttccttgtag
     6061 gaatagtcac aacgggggcc ttcggtcagc cgcggtcggg ccgcgcggca tcggccgcct
     6121 cgccgtcttc gggcggcggc aacgtgcaga gatactcggc gacgaggccc gcggcgagaa
     6181 gcagcgcggc ggccacgatg aaggccacat cgagcagcac caggctgccc tgcggcagaa
     6241 gaggccgcgt gaacgcgtag accaacatac cgacggcgat ccctgtggca gccgccccga
     6301 gcaggctgct cgccttggcg agcacggcga cacgcactgc ctgaaacggg tcgatacggc
     6361 tcttcctcgt gccccgcacc gcccggtgga tggggatcgc gagccccagg gcgatcgcgc
     6421 cgatcaaaag caacgcgatc ggcagcgaac gcggtggcac gagcatcggc atgccccgcg
     6481 agacgagcgc gacctcgaga aggaatcctc cgacgaggcc ggccagcgcg actccgacga
     6541 gcacgctcgg gctgctgcgc ctcatggtgg cccggccctc aggccggccg cgccgacggg
     6601 gcaggccacg cgccgtccgc ccagtgccgg tcggcgtgcg gcgtcgcctc gtcgtcgacg
     6661 ctgcccacca ggtcggccac gggcccccgt ccgacgagca gcgcacccgg atcgacgtcg
     6721 agccacggca ccaggacgaa cgcccgttcg ccggcccgcg gatgcgggag ggtcagcgtc
     6781 gggtcgtcga ggcggcggcc gtcatagtcg atgatgtcga tgtcgagggt ccgtgcaccc
     6841 catcgcgtgg tgcgcacccg gccgtgcgcg tcttcgatgg ccgaggtcgc ctcgagcagc
     6901 gcgtacgggt gcagccgggt tctcgccaag acgaccgcgt tcaagtacgc gggcgcgttg
     6961 cggtcgacgc cgtgcagcgt gagcgccggg gtcgcgtaca aggcggagac cgcggtgacc
     7021 tcgatgcccg agcttgccgc caggtcggcg acggcacccc gcaggttctc ctcccgctcg
     7081 ccgaggttcg cgccgagggc gagcacgacg gcatgcgtgc cgcccgcccc ccgcggcccg
     7141 gcctcgcgcg cggagtctcg cccgcggtcc cggccctgca ccgcccggcc gcgttcgatc
     7201 gtgacggcga catcgtcgaa cggcacggtg atcggggcgt gcggcttgtg cagggtcacc
     7261 ctggccagac gcacggcggc gaagcccagg gcgaccgccg cgatccgttc cgcgacggtc
     7321 tcgatgaggt cgaccgggtc gcgctcgatc gcggcgacca cgccgccggc gagccggccg
     7381 tagtcgaccg tcgccgccac gtcgtcgccc gccgccgcgg cgtggatgtc gaaccagacc
     7441 gtgacgtcgg ccacgaactc ctggccgtca ttcttctcat gctggaagac cccatggtgg
     7501 gcgaacgccc gcaaacccgt cagggtgatg tggtcacccg gcacggtgtg gtccgcccag
     7561 cgtgcggcgg cggtcggtcg gtcggcgggc tgtggggtcg ttgtcgtcat cgtgcaccag
     7621 cctgccatgc ctcgaccacg tcgagggcgc gccgggtgga ggcggggtcg tgcacccgca
     7681 ccgcccaggc gcccgcgcgc gcggcgagtg cgctgatgac cgcggtgggg gcgtcgcgtt
     7741 cttccatcgg ggcgccctcc ggcaaaagcg ccccgacaaa ccgcttgcgc gaggcgccga
     7801 ccaagatggg aaaccccgtc gcgatcagcc cgtcgaggcc cgcaagcacc tgccagttgt
     7861 gccgtgcgtc tttggcgaag ccgaggcccg ggtcgaggat catctgctca tacggcatac
     7921 cgcccgcggc gagcgcgtcg acgcgggcga gaagctcctc tcgcacctct ttggccgcat
     7981 cccggtaggc ggcacgcgcg ttcatcgtgg cggaatggcc acgccagtgc atgacgacgt
     8041 agggcaggcc gctctgggcg acgacgcccg ccatcgccgg gtcggcgagc ccgccggaga
     8101 cgtcgttgac gaccgtggcg ccggcgcgca ccgcggcgtt cgccgtctgg gagcgcatcg
     8161 tgtcgatact gaccttgatg cctcgggcgc acagctcccg gatcacgggg atcgcccggc
     8221 gctgctcctc gacctggtcg acgcggaccg cgccgggtct cgtggattcg ccgccgacat
     8281 cgacgaggtc ggcgccttga cgcaccatct cgacggcgtg tgccaccgcc gcatccgtcg
     8341 tcgcgtaccg gccgccgtcg ctgaacgagt cgggcgtgac gttcagcacc cccatgatga
     8401 gggtgcgttt gccggcaggc cggccgtcgg ccgcctcgga cacgatggcg gcggagctct
     8461 caccgcgcgt cgcgccccgc atgccggcgc tcacgcggtg gccgggcgag gcttgtggaa
     8521 gggaagcttg gcgggcttgg cggccggggc cggaggcttg gccccggtgt catcgtccga
     8581 caggccggcc tccgggtggg ggggctctgg aatggtgatc ggcggccggt cggagacggg
     8641 ccggttctga ctggagagcc agagcggccg ctccggcaac ttgacgacgt cggcgaagat
     8701 ctcctcgatc cgaatgtggt cgagcgtctc cttttcgatc agctcgccgg cgagccggtc
     8761 gaggatgtca cggttggcgt tgatcacctg ccaggcctcg tcgtgcgctt tctcaatcag
     8821 cgcccgcacc tcttggtcga ccttctcggc gacgttctcc gagtagtcgc gttcctgccc
     8881 catgttgtgg ccgaggaaaa cctcgcccga gggctggccg agtttgacgg agccgatgtc
     8941 ggcgctcatg ccgtattcgg tgaccatctt gcgggcgatg ccggtggcct tctcgatgtc
     9001 gttcgacgcc cccgtggtcg ggtcgtggaa cacgacctcc tccgcgaccc ggccgcccat
     9061 cgcgtacgcc atctggtcga gcaactcttc gcgggtgacg gagtacttgt cttcgatcgg
     9121 cagcaccatc gtgtagccga gggcccggcc gcgcggcagg atcgtgatct tcgtcaccgg
     9181 atcggtgtgg cgcatcgccg cggccgccag agcatggccc ccctcgtggt aggcggtgat
     9241 gagcttctct ttgtctttca tgacccgggt gcgacgctgg gggcccgcca cgacccggtc
     9301 gacggcctcg tccaacgccc ggttgtcgat cagctgcgcg tcggaacgcg ccgtcagaag
     9361 agccgcctcg ttcagcacgt tcgcaaggtc ggcacccgtg aagcccgggg tcttgcgggc
     9421 aagcacgtcc aactccacgt tcttcgaaag cggcttgcct ttgctgtgca cctcgaggat
     9481 cttgcgccgg cccttcatgt cgggcgcgtc cacccccacc tgccggtcga aacggccggg
     9541 gcgcagaagt gccgggtcga ggatgtcggg gcggttggtg gcggcgatca agatcacgtt
     9601 cgtcttcaca tcgaagccgt ccatctcgac cagcaactgg ttgagggtct gctcccgttc
     9661 gtcgtgcccg ccgcccatgc cggcaccccg gtggcgcccc accgcgtcga tctcgtcgac
     9721 gaagatgatc gccggcgcgt tctctttcgc ctgctgaaag aggtctcgca cgcggctggc
     9781 gccgacgcca acgaacatct ccacgaaatc ggagcccgag atcgagtaga acggcacccc
     9841 ggcctcgccc gcgaccgcgc gcgccaggag cgtcttgccc gttcccggcg gcccgtagag
     9901 cagtacgccc ttcggaatgc gggcgccgac cgcctgaaac ttggccggct ccttcaagaa
     9961 ctctttgatc tcttgcagct cttcgacggc ctcatcggcg ccggcgacat cgtcgaacgt
    10021 cacctttggc gtctccttcg acaccaactt cgccttcgcc ttgccgaact gcatgacctt
    10081 gctccccccg ccctgcatgc tggagagcat cagccagaag atcaccccga tcgccagcac
    10141 gggcagcaag aagcccagtg cggagaggaa ccagttcggc tgcggcacct ggtcgttgta
    10201 gcccttcggc ggctgcgccg cgtcgatcgc cttgatgacg gcgggccccc gggcgtcgag
    10261 gaactggaat tgaaccttcg tgccggcgtt cttatacgcc ttggagagcg tcaggtcgac
    10321 cgtgttgtcg ctgccgccga tgatcttcgc gctttgcacc tgaccgctct ggatcagctt
    10381 catcccctcg taggtgggga tggacttgaa cccgggcatc gtgatcaagc tcgagccgat
    10441 ccagacggcg atcacggcga ggacgatata aaaaatcggc ccgtgaaaga tcttcttggc
    10501 gttcatcgtg ctgtaaggtt atcgcccggc ctcacgcggc gccttcgtgt tcgctgacgg
    10561 caatcgggca gtctggcaga gcgtcgccca cgcctctgtg gggcgagccg ttgcacgcgg
    10621 ttcgaccgct cccggcgtcg actattgccc atacacgcgc ggagcgagca cgccgacacc
    10681 gcgcaggttg cgataacgct cgtcgtagtc gagcccgtac cccacgacga agtcgttggg
    10741 aatgtcgaac cccacgtagc gcacctcgat gtcgacgcgc gcggcggcgg gcttacgcag
    10801 aagggcgcag atttcgacgg aggccggccc gcgggaacgc aggttggagc tcagccacga
    10861 cagggtcagc ccggagtcga tgatgtcttc gacgatcagc acgtgccggc cggagaggtc
    10921 ggcgtcgagg tctttcaaga tgcgcacgac accgctggaa tgggttccgc tgccgtacga
    10981 gctgaccgcc atccagtcca tcgtcaccgg tatgcgcagc tcgcgcgaga ggtcggccat
    11041 gaccatgact gcgcccttca agacgccgac gagcaacacg tctttgccct cgtagtcgtg
    11101 ctcgatctgg cgggcgatat ccgccagacg ggcgcggatc tgctgctcgg tgacgagcac
    11161 cgtggtgagg tcttgcgtga cgtcggtagg ttccatgcgt gcggcaacgt ctttctcgat
    11221 gaggcctcgc gggcctcgtc tgggccttca ggcgcgcccg ggagcgctga agacgaggcg
    11281 tccgccttgc ctgaccactc taacgcccgg caggtcgagg gccgcctggc cgtgccagtt
    11341 cgtgatgagg ccggcgacgg cgagcacgtg cacgcgctcc agcgacgtgc cgaactcggc
    11401 gcggacggcg gcgcggatga cacggtgccg caacgccggc ggattcgccg cgagcacgcc
    11461 gacggggatc gcgaggcccg cctcggcagg ctcgcacagg tcttgcagca actcctgcgc
    11521 gatctggtcg agcgcgtctg cgtcttcggc cagctgccct gcggtgcggg cgagggcggc
    11581 ggcgaccccc gggccgagct gcgcctcgag agtcggcagc accacgtcgc gcacgcgggg
    11641 gcgggcatat cgggcatcgg cgttcatcgg gtcgtgccag ggatgcaggc cgaggtcgtc
    11701 acaggcgcgg tgcgtcgtta cacgcggcac ctggaggaga ggcctcaggt agtgccccgc
    11761 gacgggtcgc atgccggcga gactgcgcgc cccggaaccc cgtgcgagcc cgaggagcac
    11821 cgtctccgcc tgatcgtcga gggtgtgggc gaggagcacg gcgcgggcgc cggtctggcg
    11881 cacggcttcg tcgagcgccg catagcgcgc ctggcgcgcg gccgcctcgg agtcgccccc
    11941 gaccgccacg cagcggacca gaaccggggc gaggccgagc gagcgggccg tggcggccgc
    12001 ctgggcggcc acctgggccg agccctcttg caggccgtgg tcgacgatga ccgcgcccgc
    12061 acgggcggcc atgcggggcg cctcgaacgc gacggccgcg gccagcgcca gcgagtcggg
    12121 gccgccgctc agcgcgacga ggacgagcgg cacgtcgggg gcggcgaggc tcgacggtgc
    12181 cggggccacg cgatccggtc gcgcacagcg ggggaagtgc acgccgtcgt ctgccggatc
    12241 gagaaaggcg ccgaggccct ggcgcacggc gcgccgcacg tcggcgaccg ccggcgtgag
    12301 gcgggtgcgg cgaaccgggc ccgccgacgg ccccggggcg cctgagacgc cggtgcccaa
    12361 accctccgcc gcgaccggag cggaatcggt gggagccatc aagtaacgtt attacggatc
    12421 ttcccgcacg gggcggcagg ccgccgcggc gcgcgtcacc ggcacgcggg cccgcatcac
    12481 aaacctcgga accaaggagc agggacatgg cagagtacga cgcgatcatc gagatcccgc
    12541 gcggcagccg caacaagtac gaggtagacc acgagacggg caaggtcatg ctcgaccgcg
    12601 tgctgttcac cgggttcgtc tacccggctg actacgggtt catcgagaag acgctggggc
    12661 tcgacggaga tccgctcgac gttctggtgc tcttgcaata cccgctgttc ccgggcgtcg
    12721 gcgtcagggt gcgcccggtc ggggtcttga agatgagcga cgaggccggt aacgacgcga
    12781 agatcatcgg cgtgccttac aaggacccgc gctgggcggg catccgcgac gtcgacgacg
    12841 tgcccgaggg cacccgccaa gagatcgagc acttcttcac ccactacaaa gacctcgagc
    12901 cgcagaagtg ggttcgagtc gggcggtggg gcaatgccga ggaagcgcgg acggtaatcg
    12961 ccgaggcgat cgaacgggcg aagagcgcgg cgaccgcctg aaaccggcaa cggccctatc
    13021 agtattgacc atgatggacg ccgcccggga aaggcggaaa acccagatat tcgggaa
//
LOCUS       DASTNL010000023         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_945831, whole genome shotgun sequence.
ACCESSION   DASTNL010000023 DASTNL010000000
VERSION     DASTNL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945831"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFQ96_00705"
     CDS             <1..507
                     /locus_tag="VFQ96_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEU0256338.1"
                     /translation="AAGIAQVLDAGKLFHIDLNGQRGIKYDQDLAFGHGDLYNAFALV
                     DLLENGGPAGGPAYDGPRHFDYKPSRTEDEDGVWASAAANMRMYLLLKERAAAFRADP
                     EVREALADARVAELSEPTLAAGEGYAGLLADRASFEDFDPKPYFGGRGFGFVRLQQLV
                     AEHLLGAR"
     gene            642..1925
                     /gene="xylB"
                     /locus_tag="VFQ96_00710"
     CDS             642..1925
                     /gene="xylB"
                     /locus_tag="VFQ96_00710"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEU0256339.1"
                     /translation="MVLVMGVDTSTQSCKVVVTDAATGRVVREGRATHPAGTEVDPRH
                     WWDAVQEATQAAGGMADVAAWAVGGQQHGMVALDAAGRVIRPALLWNDTRSAAAATDL
                     IAEVGAEAFARRTGVVPVASFTVTKLRWLRDHEPENAKRVAAVALPHDWLTWRLRGFG
                     PQNPALDELVTDRSDASGTGYWGQDGYDRDLVTRALGHDVLLPRVLGPREHVTDPAGR
                     KVGAGAGDNAAAALGLGAGAGDVVVSIGTSGTVFAVSETRVTDPSGTVAGFADADGGW
                     LPLIATLNAARVLDVTAGLLGVDHAGLSALALQATPGAGGLTLVPYFEGERTPNLPDA
                     TATLSGMTLRSTTRENLARAAVEGMLRGLGAGLTAIGGLGVPLQRALLTGGGAQSAAV
                     REIAPEVLGIPVDVPEPAEYVALGAARQAARLVTL"
     gene            1987..>2292
                     /locus_tag="VFQ96_00715"
     CDS             1987..>2292
                     /locus_tag="VFQ96_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0256340.1"
                     /translation="MSEPGANWAGNHRYQAERYAEPTSLDELAELVAGARSVRMLGTR
                     HSFNDIGDSDELLVGVAALPQTIAIDEAARTVTVAGGTRYGDLARTLQEAGWALHNMA
                     "
BASE COUNT          335 a          863 c          811 g          283 t
ORIGIN      
        1 gccgccggca tcgctcaggt gctcgacgcg ggcaagctct tccacatcga tctgaacggg
       61 caacgcggca tcaaatacga ccaggacctc gccttcggcc acggcgacct gtacaacgca
      121 ttcgccctcg tcgaccttct cgagaacggc ggcccggcag gcggcccagc gtacgacggc
      181 ccgcggcact tcgactacaa gccctcgcgc accgaagacg aagacggcgt ctgggcctcg
      241 gcggccgcga acatgcgcat gtacctgctc ttgaaggagc gcgcggccgc gttccgcgcc
      301 gaccccgagg tgcgtgaagc actcgccgat gcgcgcgtcg ccgagctctc cgagcccacg
      361 ctggccgccg gagaaggcta cgccgggctc ctggccgacc gcgcctcgtt cgaagacttc
      421 gacccgaagc cgtacttcgg cggccgcggc ttcggcttcg tgcgtctcca gcagctcgtc
      481 gcggaacacc tgctcggcgc ccgctaggcc cgcggcgccc gcatcgcctg ccgaacccgg
      541 atccggcagg cgatgccgaa cctcggtaac accgaattac cgaacccggg caacaccgaa
      601 tccaggcaac accgaaccta agtcgcaccg aggaggccgc cgtggtgctc gtgatgggcg
      661 tggacacctc gacccagtcg tgcaaggtcg tcgtcaccga cgcggcgacc ggccgagtgg
      721 tgcgcgaggg tcgcgcaacg catccggccg gcaccgaggt ggacccccgg cactggtggg
      781 atgcggtgca agaggccacc caagccgccg gcggcatggc ggacgtggcg gcgtgggcgg
      841 tcggcggtca gcagcacggc atggtcgcgc tcgacgcggc cggccgcgtg atccggcccg
      901 cgctcctctg gaacgacacc cgctctgcgg ccgcggcgac ggacctcatc gcggaggtgg
      961 gtgccgaggc cttcgcccgg cgcaccgggg tcgtgccggt cgcgtccttc accgtgacga
     1021 agcttcgctg gctgcgcgac cacgagccgg agaacgcgaa gcgggtggcc gccgtcgccc
     1081 tgccgcacga ctggctgacc tggcgactgc gcggctttgg gccgcagaac ccggcgctcg
     1141 acgagctcgt gaccgaccgc tccgacgcct ccggaaccgg ctactggggg caagacggct
     1201 acgaccgtga cctcgtgacc cgcgcactcg gacacgacgt gctgctgcct cgcgtgctcg
     1261 gcccgcgaga gcacgtcacc gacccggccg ggcggaaggt cggcgccggg gccggcgaca
     1321 acgcggccgc cgccctcggg ctcggcgccg gggccggcga cgtggtcgtc tccatcggca
     1381 cgagcggcac ggtcttcgcg gtgagcgaga cccgggtgac cgacccgagc ggcacggtcg
     1441 ccgggttcgc cgacgccgac ggcggctggc tgccgctcat cgcgacgctc aacgccgccc
     1501 gggtcctcga cgtcacggcc ggcctgctcg gcgtcgatca cgcgggcttg agcgccctcg
     1561 cgctccaggc gacgccgggc gccggcggcc tcaccctcgt gccgtatttc gagggcgaac
     1621 gcaccccgaa cctgcccgac gccaccgcga ccctgtcggg catgaccttg cgctcgacga
     1681 cccgggagaa cctcgcccgc gccgcggtcg agggcatgct ccggggcctt ggcgccggac
     1741 ttacggccat cggcggcctc ggggttcccc tgcagcgggc actcttgacc ggcggcggag
     1801 cgcagtcggc ggccgtgcgc gagatcgcac ccgaggttct ggggatcccg gtcgacgtgc
     1861 cggagccggc ggagtacgtc gccctcggcg cggcccgtca ggcggcgcgg ctggtcacac
     1921 tgtgacggac ggcggcggca aacccgccac gcgggccgcc acgagccgta cgagaggatg
     1981 tatgccatga gcgagccagg cgccaactgg gcgggcaacc accgttacca ggccgaacgc
     2041 tacgccgagc ccaccagcct cgacgaactc gccgagctgg tcgccggcgc gcgctcggtg
     2101 cggatgctgg gcacccggca ctctttcaac gacatcggcg acagcgacga actcctggtc
     2161 ggcgtggcag ccctgccgca gaccatcgcg atcgacgagg cggcccgcac cgtgacggtc
     2221 gcgggcggca cgcgctacgg cgacctcgcc cgcacgcttc aggaggcggg ttgggcgctg
     2281 cacaacatgg cc
//
LOCUS       DASTNL010000024         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1316997, whole genome shotgun sequence.
ACCESSION   DASTNL010000024 DASTNL010000000
VERSION     DASTNL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316997"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VFQ96_00720"
     CDS             <1..1040
                     /locus_tag="VFQ96_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0256341.1"
                     /translation="APRPHPQAWRRSRRRYVAVVVVAGVVIVVLFVTHLALGPVGITP
                     WNVLRAIAGRPSAAYQGVIIDAVRMPRSLLAPVAGAMLGLSGALIQSLTRNPLTDPGL
                     TGVTSGSVFALVLCLTFVPASVNDPALLPFAALVGGLAAVAVLYLLTRGLRDGPFAFV
                     LKGVILGGVLASASALVLVRDNGTLPTVLLWMTGSLDAKTWADWATLWPWALAAIPAG
                     LACARTGNVLALGEETAIGLGFSVTRARLALFGVAAVAAAAATAVVGAVGFVGLVGPH
                     IARRLVGSDDRRVYPLAALIGAGLVLSADTATQAAALAGPGVAGAVPVGAVTALIGAP
                     YFFYLLTRAER"
     gene            1161..1976
                     /locus_tag="VFQ96_00725"
     CDS             1161..1976
                     /locus_tag="VFQ96_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256342.1"
                     /translation="MASLVLKGLAVGYPEGSPVVSGIDLVLPDAGVTAVLGTNGSGKS
                     TLLKTIARILRPLSGAAILDGQDIHHTPTKELARRLAILGQDPPIPRDMTVRELVAYG
                     RSPHRRPLRAATDDDRARVADALEATGTVPLADTPLDRLSGGQRQRAWIAMALAQDAP
                     TILLDEPTSFLDIEHQLEVLLLLRGLADTRGKQIVMVVHDIDQALGYADHVVVLHEGR
                     VVAAGPPGEVLTPELILRVFHVRAVIRPGPGGRAHCLPYALAGREPRDAPWHV"
     gene            complement(2106..3323)
                     /locus_tag="VFQ96_00730"
     CDS             complement(2106..3323)
                     /locus_tag="VFQ96_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0256343.1"
                     /translation="MSDIVIVGGGLTAATAAKTLRGEGFDGGIRIFAGENHNPYLRPP
                     LSKEYLQGKAGLDAVWVEKAEWYGENNVQLDTGTTVTAINPAGHEVEAGGAKVHYDQL
                     LLATGCSPRHLPIPGADLDGVLTLRTLDDSDRLKEILTGGGKHVVLIGGGWIGLEIAS
                     SAKQLGNEVVVLERDPIPLGRVLGDELGTYFTQFQRGKGVDIRGSVNITGIVGENGKV
                     TGVGIEGGETVAADVVLMGVGVVPNVSLAKDAGLDVQNGITTNAALATSAPDVFAAGD
                     VAFSLHPALNEHIRSEHWANATGSGEVVAKSMLGQSVQHDMIPYFYTDQFDLGMEYSG
                     YGPLTVGAEVAYRGDPKSGEFLAFWLKDGKVVAGMNVNVWDVNEAVQGIIRRGGVVDL
                     GKLADKDVPLDEL"
     gene            complement(3540..>4635)
                     /locus_tag="VFQ96_00735"
     CDS             complement(3540..>4635)
                     /locus_tag="VFQ96_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEU0256344.1"
                     /translation="AGADFVINTIQVGGIAATKVDLETPKKYGLLQTIGDTTGVGGVF
                     RALRTFPVLAGLARDMQAVCPDAWLLNYTNPMVMNVWWLSRVAPQLKAVGLCHSVYWT
                     VHNLCKLLGVPQDRVQYRSAGVNHQAWLTEWSLDGEDLYPRLRQKIADDPELTRRTRV
                     EIFKRIGYYPTESSEHSSEYLSWFLRSPQQIERFRLTPLEYIQTSEANVAEFEDAKEA
                     LKTNRELTLNQEATEYAPQIIHSITTGKASRIHANVINHGLIDNLPEGATVEVISTVD
                     RAGITPEPFGALPVQAAALNRTYLSVAELTVEAALTGDARMVRQAVLVDPNASSSLLP
                     EDIWRLCDELTEKHRAYLPKSLGGDLEFTL"
BASE COUNT          612 a         1606 c         1620 g          797 t
ORIGIN      
        1 ccgccccccg gccccacccg caggcctggc gtcgatcgag acgccggtac gtcgccgtgg
       61 tggtggtggc cggcgtcgtc atcgtggtgc ttttcgtcac ccatctggcg ctcggcccgg
      121 tcggcatcac gccgtggaac gtgctgcggg cgatcgccgg ccggccgagc gccgcgtacc
      181 aaggggtcat catcgacgcg gtgcgcatgc cgcggtccct tctcgcgccg gtcgcggggg
      241 cgatgctcgg gctctcgggt gcgctcatcc agtcgctcac ccgcaaccct ttgaccgacc
      301 cgggcctcac cggggtgacc tccggaagcg tcttcgccct cgtcctctgc ctgaccttcg
      361 taccggcgag cgtgaacgat cccgccctgc tgccgttcgc ggcgctcgtc ggcggcctcg
      421 ccgcggtcgc cgtgctgtac ctgctcaccc ggggcctgcg cgacgggccg ttcgcgttcg
      481 tgctgaaagg cgtgatcttg ggcggggtgc tcgcctccgc ctcggccctc gtgctcgtgc
      541 gcgacaacgg cacgctgccc acggtgctct tgtggatgac gggctccctc gacgcaaaga
      601 cgtgggcaga ctgggcgacg ctgtggccgt gggccctcgc cgcgatcccg gccggcctcg
      661 cctgcgcccg cacgggcaac gtgctcgcct tgggggagga gacggcgatc gggttgggct
      721 tctccgtcac ccgcgcccgg ctcgccctct tcggggtggc ggcggtcgct gccgccgccg
      781 cgaccgccgt cgtcggtgcc gtcgggttcg tcggcctggt cggcccgcac attgcccggc
      841 gcctcgtggg gagcgacgac cggcgtgtct acccgctcgc cgcgctgatc ggcgccgggc
      901 tcgtgctctc ggccgacacg gcgacgcagg cagccgcgct cgcgggcccc ggcgtcgccg
      961 gggccgtgcc tgtgggtgcc gtcaccgcgt tgatcggcgc cccatacttc ttctacctgc
     1021 tgacaagggc cgagaggtga ggggggccac atggtgagag gacgcggggc cggcctccgg
     1081 gccggcaggg cgtcgggggc ggtgggcatg tcgacacggc gccggcgggt gtcgctggtc
     1141 cttcgaaggg tcgggtcgtg atggcgtcgt tggtgctgaa gggcctggcc gtcggctatc
     1201 cggagggctc gcctgtcgtc tctggcatcg acctcgtctt gcccgatgcg ggcgtgaccg
     1261 ccgttctcgg cacgaacggg tcgggaaagt ccacgctgtt gaagacgatc gcccgcatcc
     1321 tgcggcccct ttcgggcgcc gcgatcctcg acgggcaaga catccaccac acgcccacca
     1381 aggagctggc ccgaaggctg gcgatcctcg gccaagaccc accgatcccg cgcgacatga
     1441 ccgtgcgcga gctggtcgct tacggccgct cgcctcaccg gcggccgttg cgcgccgcga
     1501 ccgacgacga tcgcgcccgt gtcgccgacg cgctcgaggc gacaggcacg gtgccgctcg
     1561 ccgacactcc cctcgacagg ctctccgggg gccagcgcca gcgggcgtgg atcgcgatgg
     1621 ccctggcgca agacgcgccg acgatcctcc tcgacgagcc gacgtcgttc ctcgacatcg
     1681 agcatcagct cgaggtcttg ctgcttctgc ggggcctcgc cgacacgcgg ggcaagcaga
     1741 tcgtcatggt cgtgcacgac atcgaccagg cgttgggtta cgccgaccac gtcgtcgtgc
     1801 tccacgaggg gcgcgtcgtc gccgccgggc cgcccggcga ggtcctcacc cccgagctca
     1861 tcctgcgggt cttccacgtg cgcgccgtca tccgaccggg ccccggcggc cgtgcgcact
     1921 gcctgcctta cgccctggcc ggccgcgagc cgcgggacgc gccctggcac gtctgagcgg
     1981 gtgcgagggc atccggacga ggagccgtcc gcgacaaaag aacgccgcct cacccacgaa
     2041 gacggggagg cggcgttcgg gctctcgccg aagccgaccg atcgcaaggc cggccacgcc
     2101 gggcgtcaga gctcgtcgag cggcacgtcc ttgtcggcga gcttgccgag gtcgacgacg
     2161 cccccgcggc ggatgatgcc ctgcacggcc tcgttcacgt cccagacgtt cacgttcatg
     2221 ccggcgacga ccttgccgtc cttcagccag aacgcgagga actcgccgct cttcgggtcg
     2281 ccgcggtagg cgacctcggc gccgacggtg agcgggccgt agccggaata ctccatgccc
     2341 aggtcgaact gatcggtgta gaagtacggg atcatgtcgt gctgcacgct ttggccgagc
     2401 atcgacttgg cgacgacctc gccgctgccg gtcgcgttcg cccagtgctc gctgcggatg
     2461 tgctcgttca gggccgggtg cagcgagaac gccacgtcgc cggctgcgaa aacgtcgggc
     2521 gcgctggtag cgagcgccgc gttcgtcgtg atgccgttct gcacgtcgag cccggcgtcc
     2581 ttcgcgaggg acacgttcgg cacgacgccg acgcccatca agacgacgtc ggcggcaacg
     2641 gtctcgccgc cttcgatgcc gacgccggtg accttgccgt tctcaccgac gatgcccgtg
     2701 atgttgacgg agccgcgaat atcgacgcct ttgccgcgct ggaactgcgt gaagtaggtg
     2761 ccgagctcgt cgccgagcac ccggccgagc ggaatcgggt cacgttccaa gacgacgacc
     2821 tcgttgccca gctgtttcgc gctggaggcg atctcgaggc cgatccagcc gccgccgatg
     2881 agcacgacat gcttgccgcc gcccgtcaag atctctttca ggcggtcgga gtcgtcgagg
     2941 gtgcgcaagg tgagcacgcc gtcgaggtcg gcgccgggga tgggcaggtg gcgcggcgag
     3001 catccggtcg ccaagagcag ctggtcgtag tgcaccttcg cgccgccggc ctcgacctcg
     3061 tggccggccg ggttgatggc ggtcacggtc gtgcccgtgt cgagctgcac gttgttctcg
     3121 ccgtaccatt cggctttctc gacccagacg gcgtcaagcc cggccttgcc ctgcaggtat
     3181 tccttggaaa gcggcggccg caggtagggg ttgtggttct cgcccgcgaa gatgcggatc
     3241 cccccgtcga aaccctcgcc gcgcagtgtc ttggccgccg tggccgcggt caggccgccg
     3301 ccgacgatga cgatatcgct catcgctctc ccactttctt tctttccttc tgcgaatatt
     3361 tcgtgctgac tgtaagccgc ttcatattct actgtcaaag aatgtaagtt ttagaatgcg
     3421 tgtctcgcgg cgggcagaag aaggccgggg cgaggccggc atcgcatccg cctcgccccg
     3481 gccgtgtccg ggacgacccg ggcctgcctc ccgaaggccg gcacccgggt cagtgactct
     3541 cagagcgtga actcgaggtc tccgccgagg ctcttcggca ggtaggcacg gtgcttctcg
     3601 gtcaactcgt cgcagagccg ccagatgtcc tccggcagca ggctggagct cgcgttgggg
     3661 tcgacgagca ccgcttggcg caccatccgc gcatccccgg tcagcgcggc ctcgacggtc
     3721 agctcggcca ccgagaggta ggtgcggttc aacgccgccg cctgcacggg gagggcgccg
     3781 aacggctccg gggtgatgcc ggcgcgatcg acggtggaga tgacctcgac cgtcgcgcct
     3841 tcgggcaggt tgtcgatcag cccgtggtta atgacgttcg cgtggatgcg cgaggccttg
     3901 cccgtcgtga tggagtggat gatctgcggc gcgtattccg tggcctcttg gttcagggtg
     3961 agctcgcggt tggtcttcag cgcctccttc gcgtcttcga actcggcgac gttcgcctcg
     4021 gaggtctgga tgtattccag cggcgtcagg cggaatcgct cgatctgctg gggggagcgc
     4081 aggaaccacg agaggtactc cgaggagtgc tcgctcgact cggtcgggta gtagccgatc
     4141 ctcttgaaga tctccacccg tgtgcgccgc gtgagctccg ggtcgtcggc gatcttctga
     4201 cgcagccgcg ggtagaggtc ttcgccgtcg aggctccact cggtgagcca ggcctggtgg
     4261 ttgacccccg ccgagcggta ctgaaccctg tcttgcggca cgccgagcag cttgcagagg
     4321 ttgtgcaccg tccagtaaac gctgtggcag aggcccaccg ccttcagctg cggggcgacg
     4381 cgcgagagcc accagacgtt catgaccatc gggttcgtgt agttcaagag ccaggcgtcg
     4441 gggcacaccg cctgcatgtc acgggcgagg ccggccagca cgggaaaggt gcgcagcgcg
     4501 cggaagacgc cgccgactcc ggtcgtgtcg ccgattgtct gcagcagacc gtacttcttc
     4561 ggcgtctcga ggtcgacctt ggtggctgcg atgccgccga cttggatcgt gttgatgacg
     4621 aagtccgctc cggcg
//
LOCUS       DASTNL010000025         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_207583, whole genome shotgun sequence.
ACCESSION   DASTNL010000025 DASTNL010000000
VERSION     DASTNL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207583"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFQ96_00740"
     CDS             <1..522
                     /locus_tag="VFQ96_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256345.1"
                     /translation="LGIVLTRGRLRRASAVVLAIANIGQAAPAIGLIVLLAFWLGFGF
                     WAATVSLVLYAILPVLHNTIVGLNGVNRSLIEAGRGMGMSALAVLGRVELPLAVPVML
                     AGIRTALVLLVGTATLATFINGGGLGLLITTGVNLDLPTVLVVGSLLVALLALVIDWI
                     GRVVEFVARPKGL"
     gene            629..1612
                     /locus_tag="VFQ96_00745"
     CDS             629..1612
                     /locus_tag="VFQ96_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0256346.1"
                     /translation="MRRAIWAALAVACSLALSGCGLQASSAYVPQPGPGSLQKVPGAE
                     GTSITVTGKNFTEQLIMSKIAALTARASGFRVKDLSDVPGSVPVRQLMLTGGADITFD
                     YTGTAWLTYLGHQKGIPDPQKQWQAVHDADLKNGLTWGKPSRENNTYALAIRQSSKAK
                     LGGITKLSQLKDLPVKERTFCVEDEFYSRQDGFKPMLKKYGLTFGAKGKDGVPRGNVA
                     IMDTGAVYSATAHGACNFGEVFTTDGRIKALHLLVLQDDKHFFPSYASAPVFGTAVAK
                     KYPQLVKNFAAVTAKWNNALFQRLNAKVDLDGEDPASVAFDWMRSEGFITG"
     gene            1706..3214
                     /locus_tag="VFQ96_00750"
     CDS             1706..3214
                     /locus_tag="VFQ96_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEU0256347.1"
                     /translation="MSTTKVDATPGGKVGGVELNGLNLIEESERKGRPASLFWPWFAA
                     NVSVLGVSYGSFVLGFGISFWQATLVGIVGIVLSFVFCGFISLAGKRGSAPTLTLSRA
                     SFGVHGNRLPSLISWLITVGWETVLASLAVLAISTVFHELGLASGTPTKIVTLVVVIL
                     LVVGGGIYGFDVIMRMQTVITIVTAVLTIMYIVLVWGHIDFASVAAVPNGSFAHVVGG
                     FVFVMTGFGLGWVQAAADYSRYLPRRSSSRGVVGWTTFSASLAPVILIVFGLLLAASS
                     TKLDKAIAADPIGALTTILPVWFLIPFAIVAILGLVGGAVLDIYSSGIALMSTGLKIP
                     RPVAAGIDGVIMVIGAVYIVFFAGDFIGPFQAFLITLGTPIACWCGVFLADLTLRRKN
                     YHDTDLFTPRGRYGYASWPALATLVVGTAVGWGLIVNPGMPGMDWLGYLLGPLGGVKG
                     DWAFANLGVLAALVIGYLGVLLFGRARVRRQEALPDDGVPAGEHTGRHATEA"
     gene            3230..3802
                     /locus_tag="VFQ96_00755"
     CDS             3230..3802
                     /locus_tag="VFQ96_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU0256348.1"
                     /translation="MTEAAGVENAKAWLVVIDMQRIFAGPPDDWATPGYARVSEKIRA
                     LIPLFGRRVAFTRFVAPENPQGSWVPYYRQWSFALVPPNDPLYDITAEFETAGHPVVS
                     RTTFGKWGAELARATEGTDEIVLAGVSTDCCVISTALPAADAGIHVRVVADACAGVSE
                     ADHERALRAMSLYAPMIETTTVEQVLAEGA"
BASE COUNT          611 a         1368 c         1304 g          662 t
ORIGIN      
        1 ctcggtatcg tgctgacccg cgggcggttg cgccgggcct ccgcggtggt tttggcgatc
       61 gcgaacatcg ggcaggccgc gcccgcgatc gggctcatcg tgttgctggc gttctggctc
      121 ggcttcgggt tctgggccgc cacggtctcg ctcgtgctct acgcgatcct gccggtgctg
      181 cacaacacca tcgtcgggct gaacggggtg aaccgcagcc tcatcgaggc cggccgcggc
      241 atggggatga gcgccctcgc cgtgcttggt cgcgtggagc tgccgctcgc cgtaccggtg
      301 atgctcgccg ggatccgcac ggcgctggtg ctgctcgtcg gcacggcgac gctggcaacg
      361 ttcatcaacg gcgggggcct gggcctgctc atcacaacgg gcgtgaacct cgacctaccg
      421 acggtgcttg tcgtcggctc gctgctcgtg gcgctcttgg cactcgtcat cgactggatc
      481 gggcgcgtcg tcgagttcgt cgcccgcccc aaagggctgt gaggcccccg cggcctgcag
      541 ggccacacgc gagaccggaa ggaccgaatc cagcatgacg tcgcagcgag cacacgaggc
      601 caccacacgc aaagccggcc ggcgcaccat gcgacgggca atctgggccg cgctggccgt
      661 ggcctgcagt ctggcgctct ccggctgcgg actccaggcc tcctccgcct acgtgccgca
      721 gcccgggccg ggcagcctgc agaaggtgcc cggggccgag ggcacgtcga tcacggtgac
      781 cggcaagaac ttcaccgaac agctcatcat gagcaagatc gcggcgctga ccgcccgggc
      841 ctccggtttc cgcgtgaaag acctcagcga cgtgcccggc tccgtgccgg tgcgccagct
      901 gatgctgacc ggcggggccg atatcacgtt cgactacacc ggcaccgcgt ggctgaccta
      961 tctgggccac cagaagggca ttccggaccc gcagaagcag tggcaggccg tgcacgacgc
     1021 cgacctgaag aacgggctga cgtggggcaa gccctcccga gagaacaaca cctacgccct
     1081 cgccatccgc cagagcagca aggcgaagct cggcggcatc acgaagctgt cacagctgaa
     1141 agacctcccc gtcaaggagc gcacgttctg cgtggaagac gagttctact cccggcaaga
     1201 cggcttcaag cccatgctga agaaatacgg gctcaccttc ggggcgaaag gcaaagacgg
     1261 ggtgccgcgc ggcaacgtcg ccatcatgga cacgggcgcg gtctacagcg ccaccgcgca
     1321 cggcgcctgc aacttcggcg aggtgttcac caccgacggc cgcatcaagg cgttgcacct
     1381 cctcgtgctg caggatgaca aacacttctt tccctcgtac gcctccgcgc cggtcttcgg
     1441 cacggcagtg gcgaagaagt acccgcagct ggtgaagaat ttcgcggccg tcaccgcgaa
     1501 atggaacaac gcgctgttcc aaagactcaa cgccaaggtg gacctcgacg gcgaagaccc
     1561 tgcgagcgtc gccttcgatt ggatgcgttc agaaggcttt atcacaggtt aagtgcgtgt
     1621 tataacacat catcgtcttc cctgtcccga tattcctggt agtttttcag caatcgacac
     1681 gctcgcaaag cacgggaagg gacccatgtc aaccacgaaa gtcgatgcga cacccggcgg
     1741 caaggtcggt ggcgtcgagc tgaacgggct caacctcatc gaggagagcg aacgcaaggg
     1801 tcggccggcg agcctcttct ggccatggtt cgccgcgaac gtttcagtgc tgggcgtcag
     1861 ctacggctcc ttcgtgctcg gcttcggcat ctccttctgg caggcgaccc tcgtcggcat
     1921 cgtcggcatc gtcctctcgt tcgtgttctg cggtttcatc tcgctggccg gcaagcgggg
     1981 ctccgcgccg accctgaccc tcagccgcgc ctccttcggc gtgcacggca accggcttcc
     2041 gtcgctcatc tcgtggctca tcacggtcgg ctgggagacg gtgctcgcct ctctcgcggt
     2101 gctcgcgatc tcgaccgtgt tccacgagct cggcctggcc agcggcacgc cgacgaagat
     2161 cgtgacactc gtcgtcgtga tcctgctggt cgtcggcggc ggcatctacg gcttcgacgt
     2221 catcatgcgc atgcagaccg tcatcacgat cgtcaccgcc gttctgacca tcatgtacat
     2281 cgtcctcgtg tgggggcaca tcgacttcgc ctccgtcgcg gcggtgccga acggctcgtt
     2341 cgcccacgtc gtcggcggat tcgtcttcgt catgaccggg ttcgggctgg gctgggtgca
     2401 ggcggctgcc gactactccc gctacctgcc gcgccggtct tccagccggg gcgtggtcgg
     2461 ctggaccacg ttcagcgcct ccctcgcccc ggtcatcctc atcgtcttcg gcctgctcct
     2521 ggccgcctcg tcgaccaagc tcgacaaggc gatcgcggcc gacccgatcg gcgcgctgac
     2581 gacgatcctg ccggtgtggt tcctcatccc gttcgcgatc gtcgccattc tcggcctcgt
     2641 cggcggcgcc gtgctcgaca tctactcctc cggcatcgcc ctcatgagca ccggcctgaa
     2701 gattccgcgg cccgtcgcgg cgggcatcga cggcgtgatc atggtgatcg gcgccgtcta
     2761 catcgtgttc ttcgccggcg acttcatcgg cccgttccag gccttcctca tcacgctggg
     2821 cacaccgatc gcctgctggt gcggcgtgtt cctcgccgac ctgactctgc ggcgcaagaa
     2881 ctaccacgac accgacctct tcaccccgcg cggccgctac ggctatgcga gctggccggc
     2941 cctggcgacg ctcgtcgtcg gcacggcggt cggttggggg ctcatcgtca accccggcat
     3001 gccgggcatg gattggctgg gctacctgct gggcccgctc ggcggggtga agggcgactg
     3061 ggcgttcgcc aacctcggcg tcttggccgc gctcgtgatc ggctacctcg gtgtgctgct
     3121 cttcgggcgg gcgcgggtgc gccgacaaga ggcgctgccc gacgacggcg tgccggcggg
     3181 cgagcacacc ggccgacacg ccacagaggc gtgacgaggg gagaccgtcg tgactgaggc
     3241 cgcaggggtg gaaaacgcga aggcgtggct cgtcgtcatc gacatgcagc gcatcttcgc
     3301 cggcccgcca gacgactggg cgacacccgg ctacgcgcgg gtctccgaga agatccgcgc
     3361 gttgatcccg ctgttcgggc ggcgggtcgc cttcacccgg ttcgtcgcgc ccgagaaccc
     3421 gcagggctcc tgggtgccgt attaccggca atggtcgttc gcgctcgtgc cgccgaacga
     3481 cccgctctac gacatcaccg ccgagttcga gacggcgggg caccccgtcg tcagccgcac
     3541 caccttcgga aagtgggggg cggaattggc acgggcgacc gagggcaccg acgagatcgt
     3601 gttggccggc gtctccaccg actgctgcgt catctccacg gcgctgccgg ccgccgacgc
     3661 cggcatccac gtgcgggtcg tggccgacgc ctgcgcgggg gtgagcgagg ccgatcacga
     3721 gcgcgccttg cgggccatga gcctctacgc gccgatgatc gagacgacga cggtcgagca
     3781 ggttctggcc gaaggagcat gacgacacca gggcgaaaac ccgaacgaga agaagaaccg
     3841 ccgacggcgg cgcgggcgga tgcaaggcgg gcgaccacgc agggccacgc cttggccgcg
     3901 ctgcagggcc tcgcgatcgg cgacgcgctg ggcatgccga cgcaa
//
LOCUS       DASTNL010000026         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_578446, whole genome shotgun sequence.
ACCESSION   DASTNL010000026 DASTNL010000000
VERSION     DASTNL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578446"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="VFQ96_00760"
     CDS             <1..1909
                     /locus_tag="VFQ96_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0256349.1"
                     /translation="EIAALLQRTLAEILGLKPGFSSGRGGSMHLRWHEAGALGTNAIV
                     GGGVPFAAGNAWGQRRAAQPGPGEQGPAAAASAGAGSASGSDVTVAYFGDGAANIGSV
                     LETMNLAAAWKLPLCFFVENNLYAVSTHVTEVTADERLSARAPGFGIPAWRVDGMDPL
                     AVHLAMEKARERMRAGEGPALVEAQVYRYFHQNGPYPGSAFGYRTKDEETAWRRRDPI
                     TRVEAELLRRGTLDAEGQAAVRAQAQEAVTRAIGALTEPDPDAPEAKGGARRIRPELW
                     PDPAFVDVGIRGDLSELAGARVGDGSGPSHPGKFVEAVAAVMDRRMAEDPRIIVLGED
                     VHHLGGGTNGATKGLAAKYPGRVLGTPISENAFVGLGGGIALDGRYRPVVEFMYPDFL
                     WVAADQVFNQIGKVRHMYGGGDTVPLVLRTKVAMGTGYGSQHSMDPAGIFATSAGWRI
                     MAASTPADYVGLMNIALALEDPVLMIEHVDLYGKRGEIPDDLDYQLPFGKAAIRRPGE
                     DVTVLSYLSMVAHCAEAIEETGVDAELIDLRFLDRASLDWESVGESIRKTNSVLIVEQ
                     GAVGTSYGAWLADELQRRFFDWLDQPVLRVTGSGTSPSISKVLERAAIARTEEVVAGL
                     VKAREGFGGRN"
     gene            1911..>2439
                     /locus_tag="VFQ96_00765"
     CDS             1911..>2439
                     /locus_tag="VFQ96_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU0256350.1"
                     /translation="MAGVIRMPSVLAGAEEGAIAAWLVAEGDEVAVGDTIAEVETDKA
                     TVEYQAEEAGVVGRILVPVGAVVPVGDPIVVLVGAGEDAAAVERELGLSAAASPEQDS
                     AGTSGADGVGPAGRGVGAAPAAGRAGRTRIFASPVARKLAREQGIDVGSVEGTGPDGR
                     ITRRDVEKVLRTVRQV"
BASE COUNT          365 a          811 c          904 g          359 t
ORIGIN      
        1 cgagatcgcg gcgcttctgc aacgtaccct cgccgagatc ctcggcctga agcccggctt
       61 ctcgagcggc cgcggcggct cgatgcacct gaggtggcac gaagccgggg ccctcggcac
      121 gaacgcgatc gtcggcggcg gcgtgccgtt cgcggcaggc aacgcgtggg gccagcgtcg
      181 tgccgcgcaa ccgggtccgg gcgaacaggg gccggcggcc gcggcttcag caggggcggg
      241 cagcgcctcg gggtcggacg tcactgtcgc gtacttcggc gacggcgccg cgaacatcgg
      301 ctcggtgctg gagacgatga atctcgctgc ggcatggaag ctgccgctgt gtttcttcgt
      361 cgagaacaac ctctacgccg tctcgaccca cgtgaccgag gtcaccgccg acgagcgtct
      421 ttcggcacgg gcgccgggct tcggcatccc cgcctggcgg gttgacggca tggatccgct
      481 cgccgtgcac ctggcgatgg agaaggcgcg ggagcggatg cgtgcgggcg agggcccggc
      541 gctcgtcgag gcgcaggtgt accgctactt tcaccagaac ggcccctacc cgggcagcgc
      601 gttcggctac cgcaccaaag atgaggagac tgcgtggcgc cggcgtgacc cgatcacccg
      661 cgtcgaggcg gagttgctgc gccgcggcac gctcgacgcc gagggtcagg ccgcggtgcg
      721 cgcgcaggct caagaggccg tcacccgggc gataggcgcg ctgacggagc ccgacccgga
      781 tgcccccgag gcgaaaggtg gcgcgcgccg tatccgcccc gagttgtggc ccgacccggc
      841 cttcgtcgac gtcggcatcc gcggcgacct ctccgagctg gcgggcgccc gcgtcggcga
      901 cggttccggc ccctctcacc cgggcaaatt cgtcgaggcc gtcgccgccg tgatggatcg
      961 gcgcatggca gaggacccgc gcatcattgt gctcggcgaa gacgtgcacc acctgggcgg
     1021 cggtacgaac ggcgcgacca agggtctcgc ggcgaagtac ccggggcggg tgctcggcac
     1081 gccgatcagt gagaatgcgt tcgtcggcct gggcggcggc atcgccctcg acggccgcta
     1141 ccggcccgtc gtcgagttca tgtaccccga ctttctctgg gtcgcggccg accaggtctt
     1201 caaccagatc ggcaaggtgc gccacatgta cggcggcggt gacacggtac cgctcgtctt
     1261 gcgcaccaag gtcgcgatgg gcaccggata cggctcgcag cattcgatgg atccggccgg
     1321 catcttcgcc acgagcgccg gttggcggat catggccgcc tcgacgcccg ccgactatgt
     1381 cggcctcatg aacatcgccc tcgcgttgga agacccggtc ttgatgatcg agcatgtcga
     1441 cctgtacgga aagcgcggcg agatccccga cgacctcgat tatcagctgc cgttcggcaa
     1501 ggctgcgatc cgccgacccg gcgaagatgt cacggtgctc agctacctgt cgatggtcgc
     1561 ccattgtgcc gaggccattg aggagaccgg ggtggatgcg gaactcatcg acctgcgttt
     1621 cctcgaccgc gcgagcctcg actgggagag cgtgggcgag agcatccgca agaccaacag
     1681 tgtgctcatc gtggaacagg gcgccgtggg cacctcatac ggcgcctggc tcgccgacga
     1741 gctgcagagg cgcttcttcg actggctcga ccagcccgtt ttgcgcgtga ctggctccgg
     1801 aacctcgccg agcatctcca aggttctgga gcgggcggcg atcgcccgca ccgaggaggt
     1861 cgtggcgggc ctcgtcaagg cgcgcgaagg cttcggggga aggaactgac atggccggcg
     1921 tcatccgcat gccgagcgtc ttggccggtg ccgaagaggg tgcgatcgct gcctggctgg
     1981 tcgccgaggg cgacgaggtc gcggtcggcg acaccatcgc cgaggtcgag accgacaagg
     2041 cgaccgtgga gtaccaggcg gaggaggccg gggtcgtcgg gcgcatcctc gtgcccgtgg
     2101 gggccgtcgt gccggtcggc gatccgatcg tcgtgctcgt gggggccggc gaagacgcag
     2161 ccgcagtcga gcgggagttg ggactgtcgg cggcggcgag ccccgaacaa gattcggccg
     2221 gtacctctgg tgccgatggt gtcggcccgg cgggcagagg tgtcggcgcg gcgccggcag
     2281 cgggccgggc gggccgcaca cggatcttcg caagcccggt agcccgtaag ctcgcccgcg
     2341 agcaggggat cgacgtgggc tccgtcgagg gcaccggccc ggacgggcgc atcacccggc
     2401 gagacgtcga gaaggtgctg cgcaccgtac ggcaggtat
//
LOCUS       DASTNL010000027         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1505730, whole genome shotgun sequence.
ACCESSION   DASTNL010000027 DASTNL010000000
VERSION     DASTNL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505730"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VFQ96_00770"
     CDS             complement(<1..1818)
                     /locus_tag="VFQ96_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0256351.1"
                     /translation="MAKQRPARPRPALTPGGVAYALKRALLGKPLITRQLSGQKLSKK
                     VALGVLSPDAISSAAYGPEQILIELLPAAGLAAFALALPITGVILVVLVLVAASYRQV
                     VMAYTRQGGSYVVTRANFGPRVAQVAAAALLIDYVVTVAVQTAAGTVAVVSAVPALGP
                     YSLEITIGVVVVLCYVNLRGLREAGKPFAVPTYFFVLMVGGMIVTGVVRILFFGGLPQ
                     YDPSAMAGVVPIGHGDGLVAGATVLVLLRAFANGGSSLTGVEAISDAVSAFQPPRGVN
                     ARRVLFTMASILGFLLAGIAFLAYVTHATPYQSGYPSVLSQEARAVFGNGAAGHVLYG
                     LVQASSALILYTGANTSFNGFPFLASFVASDRFLPRQLTKRGHRLVFSNGIIVLAVLA
                     IALLIVSGGSVTALVPFYAIGVFTGFAMAGYSMTKYHRTHREQGWRRKQAINLGAGVM
                     STIVVLVFAVAKFTEGAWVVVVIFPLLVLVLMRLNREYRAEAAVLDRVTRREVAAGTY
                     THHSVYVFVNALDLATIEALRYAATLRADEVTAVHFVLDTQFAERLRTRWQSLRVAVT
                     LREIDVPDRRLARAARALIVQKLKGTPGTPGVTVLLPRRS"
     gene            2118..>2979
                     /locus_tag="VFQ96_00775"
     CDS             2118..>2979
                     /locus_tag="VFQ96_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256352.1"
                     /translation="MGNVVAPAEQSGRDAVAAHPLLMFSTVALALLMSSLDSTIVATA
                     LNSLEHDLDAPITWTGWTITAYSLGLMLVLMLSGRLSERYGRRRVFLVSVVVFTVSSL
                     LCALSGNIYMLVVLRFVQALGGAGFTPSATGIIVDHFGPGRDKAVGLFGSIFPIGGMA
                     GPVLGGLFIDYWSWRGIFFVNVPIGILLLVLCLRFVPKDLPGTRGPGRVDVTGTILLG
                     LTVLGFMFALNLVGGDPHAVASAPFAAFAVLAVAAAVAFARHIRRSPSPIMPPRFVVG
                     PGFGAVNIVNM"
BASE COUNT          478 a         1007 c         1037 g          457 t
ORIGIN      
        1 gctgcggcgc ggcaacagca cggtcacgcc gggtgtgccc ggtgtgccct tcagcttctg
       61 cacgatcagg gcccgggcgg cgcgcgccaa gcgccggtcg ggcacgtcga tctcccgcag
      121 agtgacggcg acgcgcaggg actgccaacg ggtgcgcagc cgctcggcga actgcgtgtc
      181 gagaacgaaa tgcaccgccg tcacctcgtc tgcccgcaag gtcgccgcgt agcgcagcgc
      241 ctcgatggtg gcgaggtcga gtgcgttgac gaacacgtag acgctgtggt gcgtgtacgt
      301 gccggccgca acctcgcggc gggtgacccg gtcgagcacc gccgcctccg cccggtattc
      361 ccggttcagc cgcatcaaga cgagcacgag gagcgggaag atgacgacga cgacccaggc
      421 gccctcggtg aacttcgcca cggcgaagac cagcacgacg atcgtcgaca tgacgccggc
      481 acccaggttg atggcctgct tgcgccgcca accctgctcc cggtgggtgc ggtgatattt
      541 cgtcatgctg tagccagcca tcgcgaagcc ggtgaacacg ccgatcgcat agaacggcac
      601 caacgcggtg acgctgccgc cagagacgat gagcaacgcg atcgccagca cggccaagac
      661 gatgatgccg ttcgagaaca cgagccggtg cccgcgcttc gtcagctgcc gcggcaagaa
      721 gcggtcggag gccacgaagc tcgcgaggaa cgggaagccg ttgaaactcg tgttcgcgcc
      781 ggtatagagg atgagcgccg acgaggcctg cacgaggccg taaagcacgt gccctgcggc
      841 cccgttaccg aagaccgccc gcgcctcttg ggagagtaca gacgggtaac ccgactggta
      901 gggggtcgcg tgcgtgacgt aggccaggaa ggcgatgccg gcgaggagaa agccgaggat
      961 gctcgccatg gtgaacagca cgcggcgggc gttgacgcca cgcggcggct ggaaggcgga
     1021 gaccgcgtcg gagatcgcct ccaccccggt cagggaggag ccgccgttcg cgaacgcacg
     1081 caaaagcacg agcacggtcg cgcccgcgac gaggccgtcg ccgtggccga tcggcacgac
     1141 gcccgccatc gcgctcggat cgtattgcgg cagcccgccg aagaacaaga tgcgcacgac
     1201 gccggtgacg atcatgccgc cgaccatcag cacgaagaag tatgtcggta ccgcgaacgg
     1261 ctttcccgcc tcccgcaacc cgcggaggtt cacatagcag agcacgacca cgacgccgat
     1321 cgtgatctcg aggctgtagg gcccgagggc cggaaccgcg gagacgacgg cgacggtgcc
     1381 ggccgcggtc tgcacggcga ccgtgacgac gtagtcgatc agcagtgcgg ccgccgcgac
     1441 ctgcgcgacg cgcggcccga agttggcacg ggtgaccacg taggagccgc cctggcgggt
     1501 gtaggccatg accacctggc ggtacgaggc ggcaacgagc accagcacga cgaggatgac
     1561 cccggtgatg ggcagggcaa gggcgaaggc ggcgagcccg gccgccggca ggagttcgat
     1621 gagaatctgt tccggcccgt aggcggccga cgagatcgcg tcgggcgaga gaaccccgag
     1681 ggcgaccttc ttcgatagtt tctggcccga gagctgcctc gtgatgagag gcttaccgag
     1741 cagggcgcgt ttaagcgcgt aggcgacacc cccgggcgtc agtgccgggc gaggccgggc
     1801 gggtcgctgt tttgccacag cacaccatcg tatgcgtctg cgagaaaatc gcgtgcggcc
     1861 tccaccggca cggcgcgggg ctgcgctggc ccgcgtgacg cgccttgcat cccgccgccg
     1921 ccggcccgcc cgcccgcccg ctaggcagcg gacggttccc agcggcccat aggagaagtc
     1981 tcggcaaccc cgcaggcttt ccggatgcgg cggcgaggaa ctagcattaa aaacggcgtc
     2041 cgggcccatg acgtttcgga cggtatgaca gatcgtctgc cgccggtgga tgacgcgtgg
     2101 gcctggaggg ttgttcggtg ggtaacgtcg tggcgccggc cgagcaaagc ggccgggacg
     2161 ccgtggccgc gcacccgctg ctcatgttct ccaccgtcgc gttggcgctc ttgatgagct
     2221 ccctcgactc gaccatcgtc gccaccgcat tgaattcgct ggagcacgac ctcgacgcgc
     2281 cgatcacctg gacggggtgg acgatcaccg cgtattcgct gggcctgatg ctcgtcttga
     2341 tgctttccgg ccgcctcagc gagcggtacg ggcgccggcg cgtgttcctc gtctcggtgg
     2401 tcgtcttcac cgtctcttcg ctcctgtgcg cgctctctgg caacatctac atgctcgtcg
     2461 tgctgcgttt cgttcaggcc ctgggtggcg cggggttcac gccctcggcg acgggaatca
     2521 tcgtcgacca cttcgggccg ggccgcgaca aggcggtcgg gctcttcggc agcatcttcc
     2581 cgatcggcgg catggccggg cccgtcttgg ggggcctctt catcgactac tggtcgtggc
     2641 ggggcatctt cttcgtcaac gtgcccatcg ggatcctcct cttggttctc tgcctgcgtt
     2701 tcgtgccgaa agacctgccg ggcacccgcg gccccggccg ggtcgacgtg accggcacga
     2761 tccttctcgg gctgacggtg ttgggcttca tgttcgccct gaaccttgtc ggcggcgacc
     2821 cccacgcggt cgcgtcggcg ccgttcgcgg ccttcgcggt actcgccgtg gcggcggccg
     2881 tggccttcgc ccggcacatc cgtcgcagcc cctccccgat catgccgccg cgcttcgtcg
     2941 tcggccccgg cttcggcgcg gtcaacatcg tcaacatgg
//
LOCUS       DASTNL010000028         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1320681, whole genome shotgun sequence.
ACCESSION   DASTNL010000028 DASTNL010000000
VERSION     DASTNL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320681"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /gene="glmS"
                     /locus_tag="VFQ96_00780"
     CDS             <1..1481
                     /gene="glmS"
                     /locus_tag="VFQ96_00780"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU0256353.1"
                     /translation="AAWLLAREYRSRHDLGAAMRATAARLEGAFTLVAMHREHPGLVV
                     AARRDSPLVVGIGEGEVFLASDVAAFADNTRHALALGQNQVVAATPEGIVVTDLAGRP
                     AEPEPFEVDWDPAAAQKGGWPSFMAKEIDEEPAAVTDTLRGRLDGGRVRLPELEGLLA
                     AGFSVRKILILACGTAYYAGEAARHALEAWAGIPTSVELAHEFRYREPLVEEGTLVVT
                     ISQSGETMDTLMAARYARERGALTLSVCNTQGSSIPRESDAVLYTRAGPEVAVASTKA
                     FVAQVVALLLVALHLAGRVPQTGAARGGLSDLHAAAIESLLKIPGQIRTVLDTQRPAV
                     DAVAAKLAAVPSIFLLGRGVLLPVAREGALKLKETSYVHAEAFPAGELKHGPIALIEP
                     GRPVIALAPPQGTLLGAKMDSTVQELRARGADVTVIGQVGGVPADDGPAGSGRVVIPE
                     TDPLLSPLLAVVPLHMLAMAVAGVRGVDVDQPRNLAKSVTVE"
     gene            complement(1572..2618)
                     /locus_tag="VFQ96_00785"
     CDS             complement(1572..2618)
                     /locus_tag="VFQ96_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEU0256354.1"
                     /translation="MTKPATPDTSQHRRARGTHDAAPALIGVDWGSSRLRAYLLTADG
                     RVLETLTRDVGILGTPVARQREVLSRALAPWLRRHPAMPIVAAGMIGSSSGMVETSPV
                     AAPADAAKVAAGVSHTALADGTDVVVLPGLVDRHGDHDDLLRGEEAQILGWLASPDAV
                     PEAVLVLPGTHSKWVRIAAGTIRGFHTFPTGELFATLRETPSLKSAREARGGQAASKT
                     PGDATPGRRPAVNAAAFRDAVTRGLASPGLSHDLFGLRVTALARGAGHAATPPAAPFP
                     LDDALSGLLLGHEIHEATGRGLLAERLPVVVVGDLPLSERYRTALEVASLEATVADAE
                     RCVARGIATVHALA"
     gene            complement(2625..3263)
                     /locus_tag="VFQ96_00790"
     CDS             complement(2625..3263)
                     /locus_tag="VFQ96_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0256355.1"
                     /translation="MDSTLSKAASSAAAAIRETKTIAIIREKSEEAARQEATRLLGCG
                     VRVVEVSLVTPGALDVLRWMNDSLHSAGRHFGAGTVLTADDARAAKKAGAEFIVSPIS
                     SLALLGAAHEAGMTSVVGALTPTECHEAATGGADFVKLFPASLWSPKDMKGLLQALPT
                     LRLVPTGGVTLDSATRWIEAGAVALGLGGTLRRAGDAAVAALLADLAATSVG"
     gene            complement(3275..4447)
                     /locus_tag="VFQ96_00795"
     CDS             complement(3275..4447)
                     /locus_tag="VFQ96_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256356.1"
                     /translation="MTRMDAFPLWSDGEAWVSAPFDHGDRGILPHDGSWMAGDLPAIT
                     WFLARQAGDAVLPQGQGAGRDRDTPHDDAVTQAKAWSGRLANRTNITSFASVSHMFFR
                     GAFLPLVAHGAGGLRPMLERAAETISRRFLRIGYMKSFGEPDDKRYPFTTIDDVINLC
                     IPLWHAHETGDERLADAVRRSVALMADRLVRADGSTSQVLHFDEQGTAGETGTYQGFS
                     PAGCWSRGLAWGIYGFAMFHALSGEAVHREAADAMADFWMDRVQDDPAPVWDFSLPEG
                     EPVIRDSFAASLAHAGILELASWSSGERADSLRDYARTMAARLAEGYVLGHGGPGILR
                     GAALDVPHNHGVGHDCAVIVGDSYFTETLWRLRNPAGDPSILGEAARPADEASVTR"
     gene            complement(4495..>4790)
                     /locus_tag="VFQ96_00800"
     CDS             complement(4495..>4790)
                     /locus_tag="VFQ96_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0256357.1"
                     /translation="WATQVGGRDRAVRALEDAGLPAGPVRAVRDVARTEPARSRGSFA
                     QVPDPVLGTLTIVNTPFKMSYSRVGPERAAPALGADTEPVAHFLAHRSRKQNT"
BASE COUNT          651 a         1654 c         1764 g          721 t
ORIGIN      
        1 tggcggcttg gctcctcgcc cgggaatatc ggtcgcgcca cgacctgggg gccgcgatgc
       61 gcgccacggc cgcacggctg gagggtgcgt tcacgctcgt cgcgatgcac cgcgagcacc
      121 ccgggctcgt cgtggcggcc cggcgcgact cgccgctcgt cgtaggcatc ggcgagggcg
      181 aggtctttct cgcctccgac gtcgccgcgt tcgccgacaa cacccggcac gcattggcgc
      241 tcgggcagaa ccaggtcgtg gccgccaccc cggaaggcat cgtcgtgacc gacctcgccg
      301 gtcggcctgc agagccggag ccgttcgagg tcgactggga cccggcggcc gcccagaagg
      361 gcggctggcc gtcgttcatg gcgaaagaga tcgacgagga gccggccgcc gtcaccgaca
      421 cgctgcgggg gcgtctcgac ggcggcaggg tgcgcctgcc cgaactggag ggtcttcttg
      481 cggccggctt ctcggtgcgg aagatcctga tcctcgcttg cggcaccgcg tactacgccg
      541 gcgaggcggc acgccatgcg ttggaggcgt gggccggcat tccgacgagc gtcgagctcg
      601 cccacgagtt tcgctaccga gaaccgctcg tcgaggaggg cacgctggtc gtcacgatca
      661 gccagtccgg cgagacgatg gacacgttga tggccgcgcg ctatgcgcgg gagcggggcg
      721 ccctcacctt gtcggtctgc aacacgcagg gctcctcgat tccgcgcgaa tccgacgccg
      781 ttttatacac ccgggcgggg ccggaggtgg cggtcgcctc gacgaaggcg ttcgtcgcgc
      841 aggtcgtggc gctcctcctc gtggcgttgc acctcgccgg tcgcgtgccg cagacggggg
      901 ccgctcgggg tggcctctcg gatctgcacg cggcggcgat cgagagcctg ctgaagattc
      961 ccggccagat tcgcacggtg ctcgacacgc aacggccggc ggtcgacgcc gtcgctgcga
     1021 agctggccgc cgtgccgtcg atcttcctcc tcggtcgggg cgttttgctc ccggtcgccc
     1081 gcgaaggcgc gttgaagctg aaggaaacgt cgtatgtgca tgcggaggcg ttcccggcgg
     1141 gcgagttgaa gcacgggccg atcgcgctca tcgagccggg ccggccggtc atcgctttgg
     1201 ccccgcctca ggggactctg ctcggcgcga agatggattc cacggtgcaa gagctgcgtg
     1261 cacgcggtgc ggacgtcacc gtgatcggcc aggtcggcgg cgtgccggcc gatgacggcc
     1321 cggcgggttc cggccgggtc gtcatccccg agacggaccc gcttctctcg ccgcttctcg
     1381 ccgtcgtgcc gctgcacatg ctggccatgg cagtcgctgg cgttcggggg gtggacgtcg
     1441 accaaccgcg gaacctcgcc aagtcggtca ccgtcgagta ggtcaggccg ggagcatccg
     1501 ggtgggtgcg gccgcggctg ccggccggtg cgcccgggag ctcccgcggc ttcgggaagt
     1561 gtggggcgcg ctcaggcgag ggcgtggacc gtcgcgatcc cgcgggcgac gcagcgctcc
     1621 gcatccgcca cggtcgcctc gagtgaggca acctcgaggg cggtgcggta gcgctcgctc
     1681 aatggcaggt caccgacgac gacgaccggc agccgctcgg cgagaagccc ccggcccgtc
     1741 gcctcgtgga tctcgtggcc gagaagcagg ccggagagag cgtcgtccag cgggaacggt
     1801 gcggcgggtg gtgttgcggc gtgcccggca ccgcgggcga gggcggtgac ccggagcccg
     1861 aagaggtcgt gagagaggcc ggggcttgcc agcccccgcg tcaccgcgtc gcggaacgcg
     1921 gccgcattca cggcagggcg gcgccccggc gtcgcatccc caggagtctt cgacgccgcc
     1981 tggcccccgc gcgcctcccg ggcgctcttc agagacgggg tctcccgcaa ggtggcgaaa
     2041 agctccccgg tgggaaaggt gtggaacccc cggatcgtgc cggcggcgat ccgcacccac
     2101 ttgctgtgcg ttcccggcag cacgagcacc gcctccggca cggcgtcggg ggaggcgagc
     2161 caacccagaa tctgggcctc ctccccccgt aacaggtcgt catggtcgcc gtgtcggtcg
     2221 acgaggccgg gcagcacgac gacatcggtg ccgtcggcga gggccgtgtg gctcaccccc
     2281 gccgcgacct tcgccgcgtc ggcgggcgcc gccaccgggc tcgtctcgac catgccgctg
     2341 ctgctgccga tcatgccggc ggcgacgatc ggcatggccg ggtgccggcg aagccatggc
     2401 gccaacgccc ggctcagcac ctcccgctgc cgtgccacgg gcgtgccgag gataccgacg
     2461 tctcgcgtca atgtctcgag cacccggccg tcggcggtca ggaggtaggc gcgaaggcgg
     2521 ctgctccccc agtcgacgcc gatgagtgcg ggtgcggcgt cgtgcgtgcc gcgtgcgcgg
     2581 cggtgttgtg acgtgtccgg ggtggcgggt tttgtcatgg gtagtcagcc gaccgaggtg
     2641 gctgcgaggt cggcgaggag ggccgcgacc gcggcgtcac ccgcgcggcg caacgtgccg
     2701 ccgagtccta gcgcgacggc gccggcctcg atccagcggg tcgcggagtc gagggtcacg
     2761 ccgccggtcg gcacgaggcg caaggtcggt aaggcttgga gcaggccttt catgtctttc
     2821 ggggaccaca ggctggccgg gaagagtttg acgaaatccg cgccgccggt cgcggcctcg
     2881 tgacactcgg tcggcgtgag ggcacccacg acgctcgtca ttccggcctc gtgcgcggcg
     2941 ccgagcagcg caagcgagct tatggggctg acgatgaact ccgcccccgc cttcttcgcc
     3001 gcacgggcgt cgtcggcggt gagcaccgtg ccggcgccga aatgccggcc ggcggagtgc
     3061 agagagtcgt tcatccaacg cagcacgtcg agcgcgcccg gcgtcacgag cgagacctcg
     3121 acgacgcgca cgccgcagcc caggagccgg gtggcctcct gccgggccgc ttcctcgctc
     3181 ttctcacgga tgatcgcgat cgtcttcgtc tcgcggatgg ccgcggcggc gctcgaggcg
     3241 gccttgctta gagtggaatc catgacacct ccggtcatcg tgtcaccgaa gcttcgtctg
     3301 caggccgcgc ggcctcgccc aggatcgacg ggtctcctgc cgggttccgc agccgccaga
     3361 gcgtctcggt gaagtagctg tctccgacga tgaccgcaca gtcgtggccg acgccgtggt
     3421 tatggggcac gtcaagcgcc gcgccacgca ggatgcccgg cccgccgtgc ccgagcacgt
     3481 agccttccgc gagccgggcc gccatcgttc gggcgtagtc gcgcaggctg tctgcgcgtt
     3541 cgccggagct ccagctggcg agttccagga tgccggcatg ggcgaggctc gccgcgaagc
     3601 tgtcacggat gaccggctcg ccttcgggta aggagaagtc ccagacgggc gccgggtcgt
     3661 cttgtacccg gtccatccag aagtccgcca tcgcatcggc cgcctcgcgg tgcaccgcct
     3721 cgcccgacag ggcgtggaac atcgcgaaac catagatgcc ccacgcgagt ccccgggacc
     3781 agcagcccgc cggcgagaag ccttggtacg ttccggtctc ccccgccgtt ccctgctcgt
     3841 cgaagtgcag cacctgcgac gtcgagccgt cggcgcgcac cagccggtcg gccatgaggg
     3901 cgacagagcg gcgcacggcg tcggcgaggc gttcgtcgcc cgtctcgtgc gcgtgccaga
     3961 gagggatgca caggttgatg acgtcgtcga tcgtcgtgaa gggatagcgc ttgtcgtcgg
     4021 gctcgccgaa cgacttcatg tatccgatgc gcaggaaccg gcgagagatc gtctctgcgg
     4081 cccgttccag catggggcgc agcccgccgg cgccatgcgc gacgagcggc aaaaaggcgc
     4141 cgcggaagaa catgtgcgac accgatgcga aactcgtgat attggttcgg ttcgccagcc
     4201 ggccgctcca agctttcgcc tgtgtcacag cgtcgtcgtg gggcgtgtca cggtcacgac
     4261 cggcgccttg cccttgcggc aggaccgcgt cgccggcctg ccgggcgaga aaccaggtga
     4321 tcgcgggcaa gtcgccggcc atccacgagc cgtcgtgcgg caggatgccg cggtcgccgt
     4381 ggtcgaaggg ggcgctcacc caggcctcgc cgtcgctcca cagcgggaag gcgtccatcc
     4441 gcgtcatcgt gcgggtcttt gcccgaactc gttgccatgc ttcgtcgagg gtcgtcacgt
     4501 gttctgcttt cttgatcggt gggcgaggaa atgggcgacg ggttcggtgt cggcgcccag
     4561 agcgggtgcg gcccgttccg ggccgacccg ggagtaagac atcttgaacg gggtgttcac
     4621 gatggtgagt gtgccgagca ccgggtcggg aacctgggcg aacgagcccc gcgagcgggc
     4681 aggttccgtg cgggcgacgt cacgcaccgc ccgcaccgga ccggccggca ggcccgcgtc
     4741 ttcgagggcc cgcacggcgc ggtctcgccc gccgacctgc gtcgcccacg
//
LOCUS       DASTNL010000029         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_950201, whole genome shotgun sequence.
ACCESSION   DASTNL010000029 DASTNL010000000
VERSION     DASTNL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950201"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2018)
                     /locus_tag="VFQ96_00805"
     CDS             complement(114..2018)
                     /locus_tag="VFQ96_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEU0256358.1"
                     /translation="MSSTSLGGPVEPSHAVLRPLGVGEIIVTGGLWHRLQSVNAEATL
                     GHCLAWMERIGWIGNFDRAAAGTIGGDHAGIEFVDSEVYKLLEAMAWELGRKPDGRLE
                     QEYDSLVGRVAAAQQADGYLHTSFGRAGQPERYSNLEFGHELYCFGHLFQAAVARLRT
                     GHDDLLPQVARRLADHVCAAFGPDGRPAIGGHAEVEMGLVELARATGERRYLRQAQIF
                     IDRRGHDTLRHGAFGPFGAEYFQDEMPVREARTMRGHAVRALYLASGAADVACETGDK
                     ELAEAVKAQWQGGMTRRTYITGGVGSHHQDEGYGDDFELPPDRAYSETCAGIASVMLS
                     WRLLLATGDEEYADLIERTLLNNVLASPREDGKAFFYANTLHQRRPGTVPDEHALAHR
                     AEASLRAPWFEVSCCPTNVARTLASVGLYFATATDAGIQIHQYGCYQIATTLASGPVR
                     VQVETDYPFDESVKITVLEAPAADTTLSLRIPPWAGDTMRLSEPATRPTPGAAVIRRR
                     FLAGDVIELVLSVRPRVTRPDRRIDAVRGTFAVEAGPLVMALESADLPAGWSVNDIEA
                     DPLSLAGSPDGPTLDVTRCDTGDGWPYRSEAQHDTGEKRTARLIPYSTWANRGPSTMR
                     VWIPEPAAGL"
     gene            complement(2005..4014)
                     /locus_tag="VFQ96_00810"
     CDS             complement(2005..4014)
                     /locus_tag="VFQ96_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEU0256359.1"
                     /translation="MVMFTTLGHDVLEIRHRHEVIRVQAWGRDSVRVRAAQHRLPTTT
                     NGALDEPPADTPKATVSVAGDRASLTNGRLRVEVDFDLDSPYPEPLLTFRTADGHELL
                     AEQREHFWMPGARAYFGNRAGSYEIHQQFAAYTDEKLFGMGQRTHGRLDLKGLALDLV
                     QRNGEVSIPFVLSNRGYGLLWNTPAVGRVEFARNATRWQAGQAREIDYWLSAAESPAE
                     ILGRYADATGHSPELPAWASGFWQSKLRYRTQQELLDVAREYKRRDLPLSVIVADFFH
                     WSHMGDFRFDAREWPDPDAMVAELAALGVELMVSVWPTVSPLSENFTTFHDRGLLVAA
                     DQGVEFQQTIQDKDMNAPSPVAFYDPTNPATREYVWSLIEKNYLRRGIRVFWLDASEP
                     ELTPAQPANLSLYAGPGAETVSLYPKENARLFAEGMAAAGQEGTVLLCRSAWAGSQKY
                     GAAVWSGDIPATWDSLRRQVRAGLGIALSGIPWWTTDIGGFHGGDPADPGYRELMIRW
                     FQYGVFCPLFRLHGDRQPRVPTGYAMTGGPNEVWSYGDEAYEHLAAALRLRERLRPYI
                     HRAMRAASATGVPPMRPLFVDFPDDPQSWYQEDEFLFGPELLVAPILEAGQAERNVYL
                     PSGATWVDTGTGLSHPGGQWLSVATSLDRIPTYVTEGSELLHVIH"
     gene            complement(4069..>4822)
                     /locus_tag="VFQ96_00815"
     CDS             complement(4069..>4822)
                     /locus_tag="VFQ96_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0256360.1"
                     /translation="PVYWMVNVSLTSETNMRKDPPDLIPIGGTLHGYEVVLQQQLPFL
                     GTSLLIGLGTVVVTLVLAAPAAFSLAKLRPRGGAVLNFVLLIAQMIPAIIMAMGFYTI
                     YVRLGLLDSVWGLIIADSTIAVPFGVLIFTAFMIGIPDELLQAARIDGASTWRTFVSV
                     VLPVSRNSIVTVSLFSFLWAWSDFLFANTLDGGGNLKPITLGIYAYIGNNNQQWNSIM
                     ATAVVASIPAAVLLILAQRYVAAGVTAGAVKD"
BASE COUNT          755 a         1517 c         1752 g          798 t
ORIGIN      
        1 cgtgatcgtc ggcggcgccc tcgtcgtcgg ctacgtcgat gcccaccagc gccagggtga
       61 ggttcggctc tgagggaggg cctcggcggt gtgggccaga ggcgcccggt ggcttagagc
      121 ccggcggcgg gctcggggat ccacacgcgc atggtcgagg ggccgcggtt cgcccaggtg
      181 gaatagggga tgagccgagc tgttctcttc tcgccggtgt cgtgctgggc ctcgctgcgg
      241 tacggccagc cgtcgcccgt gtcacaacgc gtgacatcga gcgtcggccc gtcaggggag
      301 ccggcgaggc tgagcgggtc ggcctcgatg tcgttgacgc tccacccggc cggcaggtcg
      361 gcggattcca aggccatcac cagaggcccg gcttcgaccg cgaacgtgcc gcgcacggcg
      421 tcgattcgac gatctggccg ggtcacccgt ggtcgcaccg acagcacgag ttcgatgacg
      481 tcgcccgcaa ggaagcggcg gcggatgacg gccgcgccgg gtgtcgggcg cgtggcgggc
      541 tcggagagtc gcatcgtgtc gccggcccac ggcggtatgc gcaggctgag ggtggtatcg
      601 gcggccgggg cctcaagaac agtgatcttc accgactcgt cgaacgggta gtcggtctcc
      661 acctgcacac gaaccggccc tgaggcgagc gtggtcgcga tctgatagca cccgtattgg
      721 tggatctgga ttccggcgtc tgtcgcggta gcgaagtaga ggccaacact ggcaagggtg
      781 cgggcgacgt tggtggggca gcacgagacc tcgaaccaag gagcgcgcag gctcgcctca
      841 gcccggtgtg cgagggcgtg ctcgtcgggt accgtcccgg gccggcgctg gtgcagggtg
      901 ttcgcataga agaacgcctt gccgtcttct ctcggggagg cgagcacgtt gttcagcagg
      961 gtgcgttcta taaggtctgc gtattcctcg tcaccggtgg cgaggagaag ccgccaactg
     1021 agcatgacgg aggcgatgcc cgcgcaggtc tccgagtacg cgcgatcggg gggcagctcg
     1081 aaatcgtctc cgtagccttc gtcttggtga tgtgagccga cccctcccgt gatgtatgtt
     1141 cggcgggtca tgccgccctg ccattgcgcc ttgaccgcct cggccagttc cttgtcgccg
     1201 gtctcgcagg cgacgtcggc ggcgccggag gcgaggtaga gggcccgtac cgcgtgcccg
     1261 cgcatcgttc gggcctcgcg cacgggcatc tcgtcttgaa agtattcggc accgaagggg
     1321 ccgaaggcgc cgtggcgcag cgtgtcgtgg ccgcggcggt cgatgaagat ctgtgcctga
     1381 cgcaggtagc gtcgctcgcc ggtcgcgcgg gcgagctcga cgagccccat ctcgacctcg
     1441 gcgtggccgc cgatcgccgg gcggccgtcg gggccgaagg cggcgcagac gtggtcggcg
     1501 agccgcctgg cgacctgggg gaggagatcg tcgtgcccgg tgcgcagccg ggcgaccgcc
     1561 gcctggaaga gatgcccgaa gcaatacagc tcgtgcccga actcgaggtt ggaatagcgt
     1621 tccggctgcc ccgcccgacc gaagctggtg tgcaggtagc cgtctgcctg ttgtgcggcg
     1681 gcgacccggc cgacgagaga gtcatactct tgctcgagcc tgccgtcggg cttgcggccg
     1741 agctcccagg ccatcgcctc gagaagcttg tacacctcgg agtcgacgaa ctcgatgccg
     1801 gcgtggtcgc cgccgattgt tccggccgcc gcgcggtcga agttcccgat ccagccgatc
     1861 cgttccatcc aggcgaggca gtggccgagc gtcgcctcgg cgttcacgct ctgcaggcgg
     1921 tgccagagcc cgcctgtgac gatgatctcg cccactccga gtgggcgcag tacggcgtgc
     1981 gagggctcga ccggcccgcc gaggctagtg gatgacatga agcagttcgg atccttcggt
     2041 gacgtaggtc gggatgcggt cgaggcttgt ggcgacgctc agccactgcc ctccggggtg
     2101 ggacagcccg gtgccggtgt cgacccaggt cgcgccagag ggcaggtaga cgttccgttc
     2161 ggcctgcccg gcctcgagga tcggcgcgac gaggagctcc ggcccgaaca ggaactcgtc
     2221 ttcctggtac cacgactgtg ggtcgtcagg gaagtcgacg aacagggggc gcatcggcgg
     2281 cacgccggtg gccgaggcgg cacgcatcgc ccgatgaatg tacggtcgca ggcgttcgcg
     2341 caaccgcagc gccgcggcga gatgctcgta ggcctcgtct ccgtacgacc acacctcgtt
     2401 cggtccgccg gtcatggcgt atccggtggg cacccggggc tggcggtccc cgtggagcct
     2461 gaagagcggg cagaacaccc cgtactggaa ccaccggatc atgagttccc ggtagccggg
     2521 gtcggcgggg tcgcccccgt gaaaaccgcc gatgtccgtc gtccaccagg ggatgccgga
     2581 aagcgcgatg ccgagccccg cccgcacctg ccgccgcaac gagtcccagg tcgcagggat
     2641 gtcacctgac cacacggcgg cgccgtactt ctgtgatccg gcccacgccg agcggcagag
     2701 cagaaccgtg ccctcctggc ccgcggccgc catgccttcg gcgaacagcc gtgcgttctc
     2761 tttcgggtag aggctgaccg tctcggcgcc cggcccggca tacaggctga ggttcgcggg
     2821 ctgtgccggg gtcagttccg gctcggaggc gtcgagccag aacacccgta taccccgacg
     2881 aaggtagttc ttctcgatga gtgaccagac gtattcgcgc gtcgccggat tcgtggggtc
     2941 gtagaacgcg accggtgagg gcgcgttcat gtctttgtcc tggatggtct gctgaaactc
     3001 caccccctgg tcggcggcga ccagaagccc gcggtcatgg aaggtcgtga agttctcgct
     3061 caaaggcgag acggtgggcc agaccgagac catgagttcg acgccgagtg ctgcgagttc
     3121 cgcgaccatc gcgtcggggt ccggccactc tcgggcgtcg aaccgaaagt cgcccatatg
     3181 tgaccagtgg aagaagtccg cgacaatgac cgagagaggc aggtcgcgtc tcttgtattc
     3241 gcgggccacg tcgagaagtt cctgctgcgt gcggtagcgc agcttcgact gccagaatcc
     3301 gctggcccag gcgggcagtt ccgggctgtg gcccgtcgcg tcggcgtatc ggccgaggat
     3361 ctccgccggg ctttctgcgg cgctcaacca atagtcgatc tcgcgggcct gcccggcttg
     3421 ccaccgggtc gcgttgcggg cgaactcgac tcgtccgacg gccggtgtgt tccacagcag
     3481 cccgtagccg cggttcgaga gcacgaaggg aatgctcacc tcgccgttgc gctgcacgag
     3541 gtcgagcgcc aatcctttga gatcgagccg cccatgcgtg cgttggccca tgccgaacag
     3601 tttctcgtcg gtgtaggcgg cgaactgctg gtggatctcg taggaccctg cgcgatttcc
     3661 gaagtatgcc cgcgcgccgg gcatccagaa gtgttcgcgc tgttcagcca gaagttcgtg
     3721 tccgtcggct gtgcggaagg tcagcaacgg ttcggggtag gggctgtcga ggtcgaagtc
     3781 gacctccacg cggagccggc cgttcgtgag gctcgcccgg tcgcccgcga cagagaccgt
     3841 cgctttcggg gtgtctgccg gcggctcgtc gagtgccccg ttcgtggtcg tcggcagccg
     3901 atgctgcgcg gcgcgcaccc gcacgctgtc acgaccccac gcctgcaccc ggatgacctc
     3961 gtgccggtgg cggatctcga gcacgtcgtg accgagagtg gtgaacatga ccatggagcg
     4021 cattccttta tgaagtcgac agggatgagg gggtaggccg gcgggccgtc aatccttgac
     4081 ggcgcccgcc gtgacgcccg cggcgacgta gcgctgggcg aggatcagga gcacggccgc
     4141 cggaatggag gcgacgacgg cggtggccat gatcgagttc cattgctggt tgttgttgcc
     4201 gatgtaggca tatatgccga gcgtgatcgg cttgaggttt ccgccgccgt cgagcgtgtt
     4261 cgcgaagagg aaatcagacc acgcccagag gaaggagaac agcgagaccg tcacgatcga
     4321 gttgcggctg acgggcagca cgaccgagac gaaggtgcgc caggtgctgg cgccgtcgat
     4381 gcgggccgcc tggagcaact cgtcgggtat gccgatcatg aaggctgtga agatcagcac
     4441 cccgaaggga acggcgatgg tcgagtcggc gatgatcaac ccccagacgc tgtcgagcag
     4501 gccgaggcgc acgtagatcg tgtagaagcc catcgccatg atgatcgcgg gaatcatctg
     4561 cgcgatcagg agtacgaagt tgagaaccgc gccgccgcgg ggccgcagct tggcgaggga
     4621 gaacgcggcg ggggccgcga gcacgagggt gaccacgacc gtgccgaggc cgatcagcag
     4681 actggtgccg aggaacggca gttgctgctg cagcaccacc tcgtagccgt gcagggtgcc
     4741 gccgatcggg atgaggtcgg gcgggtcctt gcgcatgttc gtctcgctgg tcagcgagac
     4801 gttgaccatc cagtagacgg gg
//
LOCUS       DASTNL010000030        14095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1506973, whole genome shotgun sequence.
ACCESSION   DASTNL010000030 DASTNL010000000
VERSION     DASTNL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506973"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFQ96_00820"
     CDS             <1..1307
                     /locus_tag="VFQ96_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU0256361.1"
                     /translation="HGQSDQLRLRGQGAQRHALDAYGAEPVAEAAREYRAAYASWRGH
                     REELDRLSGELDARRREAEFLRDALERIADLDPAPGEDVRLAERAERLTNLEELHSAA
                     AAAREWLSSEEGEGQDVVSLVALAQSTLARAGGHDPALAALAETLGSLGYAAADAAAE
                     LSRYLDGLQPGAAEELEALHERRAALAALQRSYGPGLDDVLTLAAEGPGRLALLSGDD
                     DEIVRLRGLLTADEETLGRAAARLSARRAAAAERLGEAVTRELAALAMPDARLVVEVT
                     ALADYAAQGKDAVRMLLRPHRGAEARPLGRGASGGELSRVMLAIEVVIADADPVPTLV
                     FDEVDQGVGGAVAIEIGRRLARLARHTQVIVVTHLAQVAAFATNHLAIVKEREGALTA
                     SSVRRLDGADRVAEMTRLLSGLTGSATGARHAQELLDLAAAS"
     gene            1394..3958
                     /locus_tag="VFQ96_00825"
     CDS             1394..3958
                     /locus_tag="VFQ96_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0256362.1"
                     /translation="MRRSPARLYRHVRRAIGYETNAFWRAKPVENETVFYESFSGNGM
                     LGNPEAIFRRLIARGDTAGLHHVWALRGARHSKARAEFAGDDRVSFVEYRSAAYWRAL
                     STSGYLINNATFPPRFGKRDGQTYINTWHGTPLKRMGYDVAGGALDTANIVRNFVQAD
                     YLLAANPFMAEQMYESAYKMRGIFRGRFVEEGYPRIDRQFLDRAGGLLVRGELRAAGL
                     PVADDDKIVLYAPTWKGTKFGRPEDDVEELTATVRRIQEQVAPAGYRVLLKTHQIVHR
                     MLGKASARAHMIVPNEIATNRLLGVTSVLVTDYSSIYFDFLATGRPILFLTPDIEDYE
                     GYRGLYFPPEEWPGPLARTADEVAGFVHRIAADGLGADVEARYRAARDRFCPHEDGKV
                     TDRVIDAVFRGDRGRRVRPARDGGKTAVLFYAGGMRSNGITTSMLNLLSRLDYDRLDV
                     SVFFQKSRRAEVIAGQRAMNPNVRQFPRVGGMNGSKAAQLVRHLSYGAGRIARHRTDP
                     HQRRLWDDEWRRCFGASRFDHVIDFSGYSPFWSILLLHSPAAVRSVWLHNDMLSDAHR
                     DVRGRKPNLRGLTSEFSLYREFDHLVSVSPALADINRQALAHYAAPEAFTAAVNTVDA
                     ADIRRRGAERLEDTVRRDEEEHLPAWAIDLIGGPARTTFVCVGRVSPEKNHARLVRAF
                     EAVHARHPDTRLVIVGGGPLQGELEAHVSRVGLEDSVYLTGHQGNPYPIVAASDCFVL
                     SSNYEGQPMVLLEALVLGVPAVTVNFGSARDALPPGLGLVVPATDEGLEAGMEAYLRG
                     EVPGGVFDVAAYNQGAVEQFYRAVGLTEAHPAPHAGPGLAHPGPGPSIDKIQARGE"
     gene            3948..5651
                     /locus_tag="VFQ96_00830"
     CDS             3948..5651
                     /locus_tag="VFQ96_00830"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU0256363.1"
                     /translation="MVNSAQTEPGGTTKHIFVTGGVVSSLGKGLTAASLGNLLTARGL
                     RVVMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLNVNLSKAANVTT
                     GQVYSHVIAKERRGEYLGDTVQVIPHITDEIKRRMRLQASEEPRPDVIITEVGGTIGD
                     IESQPFIEAARQVRHELGRGNCFFVHVSLLPFIATSGEQKTKPTQHSVAALRSLGIQP
                     DALVLRSDRPVSAANRRKVALMCDVEEKAVVNTVDLPSIYDIPASLHREGLDDYIIDA
                     LGLTAGEADRSDWEALLEVVHDPKYEVTIGLVGKYVDLPDAYLSVTEALKAGGFANNA
                     KVRIVWIPSDECEGPEGQARHLSGVDGICVPGGFGIRGIEGKLGALRFARENGLPALG
                     LCLGLQCMVIEYARDVVGLAGASSSEFDPETPYPVIATMAEQVDILNGGDLGGTMRLG
                     LYPADLAAGSVVAGLYGAARVSERHRHRYEVNNAFRKRLTEAGLRFSGTSPDGSLVEF
                     VELPATSHPFYVGTQAHPELRSRPNRPHPLFRGLIEAALARQEASRLFEVEDELGAPV
                     D"
     gene            5770..6378
                     /locus_tag="VFQ96_00835"
     CDS             5770..6378
                     /locus_tag="VFQ96_00835"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0256364.1"
                     /translation="MAYHGRIWDVVKDEFPYGQATLTREYVAHPGAVAILALDEAERV
                     LLINQYRHPVRSMMWEIPAGLLDVDGESPLAAARRELAEETDLVARDWAVLGEYYTTP
                     GSSSEAIRIYLARGIAAAERPFEREAEEADIRLRWVPLEAAVTAVLGRRIQNPSAVVG
                     LLAAYAARSRGWQSLAEPDAPWERHPRWHDGRLTSRDAPARP"
     gene            6375..7271
                     /gene="xerD"
                     /locus_tag="VFQ96_00840"
     CDS             6375..7271
                     /gene="xerD"
                     /locus_tag="VFQ96_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU0256365.1"
                     /translation="MNGVEAAAEQYLRHASIERGLARNTVLAYRRDLAGYAGWLRERG
                     VTRPAAVTPRDVATFTAHLHDEGRSAASIGRALSAVRGMHRFLRDEGVVPDDATREIR
                     PPKLPARLPRVLSVDEVTRLLAATDGDRPERIRDKALLELLYATGARISEATRLDVDD
                     VVGKDLVRLRGKGAKERIVPVGSYAQAALAAYLVRVRPAWAVRKATPALFLGVRGGRL
                     SRQNAWLIIRRAAGIAGLESAVSPHTLRHSFATHLLEGGADVRVVQDLLGHSSVTTTQ
                     IYTHVTADTLRDMYTTAHPRAR"
     gene            7304..8248
                     /locus_tag="VFQ96_00845"
     CDS             7304..8248
                     /locus_tag="VFQ96_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0256366.1"
                     /translation="MRTFTMPGTDIVAPNIILGLMRIAEKSDEEVQALVHGARDNGID
                     FVDIADVYGPTLHACEARFAEAMHLTPSERDQVTIQTKAGIVPEEGDAAYFDFSYEHI
                     MASVEGSLKALRTDRIDILLLHRPDALVEPDEVARAFDELHADGKVRFFGVSNHTPRQ
                     IDLLKKSVAQPLVANQMQLSITHSPLIAQGVAANMSGLEQSVTLDGGGLLDYCRLNHI
                     TVQAWSPFQAGFFDGVFLGSERFPELNAAIDRIAAAHGVPPAAVATGWITRHPAGMQV
                     VLGTTTPSRVADSAQGSELPLTRSEWYELFRTSGHRVP"
     gene            8393..9286
                     /locus_tag="VFQ96_00850"
     CDS             8393..9286
                     /locus_tag="VFQ96_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0256367.1"
                     /translation="MARSDDTRIELGGIDLAGPVLGPTGRPLREFPEPAPLTTHGPAR
                     IIAMCNQKGGVGKTTSTINLGATMADDGRRVLAVDFDPQGALSAGLGVATHDVPTVYD
                     LLVSSHTEPGEVIQHTGIPGLDLLPANIDLSAAEVQLVGEVAREQILARVLRKVSADY
                     DLILVDCQPSLGILTVNALTAAHGVIVPLECEYFALRGVALLVETIEKVKDRLNPVLE
                     LDGILATMYDARTLHSREVLQRVVETFGDRVLETVISRTVKFPDASVAALPITRFAPE
                     HQAAAAYRQLARELIARGAVA"
     gene            9270..10106
                     /locus_tag="VFQ96_00855"
     CDS             9270..10106
                     /locus_tag="VFQ96_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU0256368.1"
                     /translation="MAPSPELAVRPGRAFRVDLGGFEGPFDLLLSLIAKHEMDVTEVA
                     LARVADEFVDYLRGLEEASGGDARSLDAASGFLVVAATLLDLKASRLLPHAEPVDAED
                     VAALEARDLLFARLLQYRAFRGAAAWLAERLDEEGRRAARAVPLEERFRKSTPELRWQ
                     TSVDDFAVVAALALAPVAPPVIPVGHLHAPRVSIRRQAEHVVRLLRSGEAHTFHDLIA
                     DAVGEGRAGLVVARFVAVLELYRRRALWLRQDDPLGDLLVRWVASDWDEASLNDLGAG
                     YE"
     gene            10099..10767
                     /gene="scpB"
                     /locus_tag="VFQ96_00860"
     CDS             10099..10767
                     /gene="scpB"
                     /locus_tag="VFQ96_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU0256369.1"
                     /translation="MNDTSLGVLPESSPREEARDESEAVSSPDAPGAGAALAADDLRC
                     RVEAILTVAETPVEPPALAAALQVSADEAQAALDALRADYDGEGPGPRRGFELRRVGG
                     GWRLYVRRDYDLAVTAFVSGRRRARLSQAALETLAVIAYKQPVTRGQVAAIRAVNVDS
                     VVHTLLAHGLITELFADEETGAINYGTTDLLLTKLGIDSLDQLPKIGPLLDDGAQGFD
                     DELR"
     gene            10754..11674
                     /locus_tag="VFQ96_00865"
     CDS             10754..11674
                     /locus_tag="VFQ96_00865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0256370.1"
                     /translation="MSSADGAAPTGPGTAGVRLQKVLAAAGVASRRASEELIAAGRVR
                     VNGATVTEQGRRVDPALDRVEVDGRPVQVDVGKRYVILNKPVGVVSTLHDEDGRPDLA
                     VFAGRYPERLFNVGRLDAATSGLLLLTNDGELAHVLMHPSFGVEKTYVARVRGAVTPR
                     VVARLTAGIDLDDGPIAADTARLLQTGGGESLVEVSLHSGRNRIVRRMFAAVGHPVLG
                     LVRRSFGPLALTTLPAGQTREATRAEVSALLALARDAHDEGSRGGRGRRDGGEPAPRR
                     ARRPASGGYRGRGGKKPQRHRNSRRPGRTA"
     gene            11823..12929
                     /locus_tag="VFQ96_00870"
     CDS             11823..12929
                     /locus_tag="VFQ96_00870"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU0256371.1"
                     /translation="MASTTNARLTGPVRVVGAGLLGASIGLGLRSLGVDVVLADASPA
                     ALRLAVDYGAGRAALAGDRPALVVVCVPPDRTGEVVAAELVSWPDAVVADVASVKAPV
                     LRDIVSCLGAAHAGALMRYVGSHPLAGRERGGPIAARADLFLGRPWVVCAPEGTALGP
                     VGVVEDLILDLGATPVTLDAATHDEAVAAVSHAPQLVASLMAKQLLEPPAVALGLAGQ
                     GVRDVTRIAASDPELWVQILAANAPAVGRILQALRTDLDRVLQALGDPDAPGARRALA
                     GTLAAGNTGVARLPGKHGLDRRYTSVVVLVDDVPGELGRLLTELGDIGVNMEDLRLEH
                     SPEAEVGLAEISVLPQAAQQAIDELTARGWRITG"
     gene            12926..13801
                     /gene="cmk"
                     /locus_tag="VFQ96_00875"
     CDS             12926..13801
                     /gene="cmk"
                     /locus_tag="VFQ96_00875"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013398807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU0256372.1"
                     /translation="MNDTQKVGERMPASGGGGAGAPVFVVAIDGPAGSGKSSVSKAVA
                     GLLGFAYLDTGAVYRALTWLALRRRVEPGDPDALAALLAAFRDVRIDTDPADYRVRVA
                     QTDVTDEIRTPEVTGRVSALARVPAVREHVNGLFRRLIAETARPGIIVEGRDITTVVA
                     PDAPVRLLLTADAAVRAARRVGELAGAAGRDAAGALHRRDAADSRVVDFMNAADGVAT
                     LDSTRLDFAATVAAVRRIVVDARAATQAQARAVARASAKAQAGTPPHAGAGGAGQSAG
                     RRPGEAGLETNGDDS"
     gene            13798..>14095
                     /locus_tag="VFQ96_00880"
     CDS             13798..>14095
                     /locus_tag="VFQ96_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU0256373.1"
                     /translation="MTDGDAPIARDGDPNRGGTQSLGGSPDTEAGAERTRALRSGLDA
                     YELEESDLAVLDGSPAQAAEAVPVLPAVAIVGRPNVGKSALVNRILGRREAVVED"
BASE COUNT         2033 a         5126 c         4871 g         2065 t
ORIGIN      
        1 tccacggcca gtcggaccag ttgcggctgc gcggccaagg cgcgcagcga cacgctctcg
       61 acgcgtacgg cgcagagccg gtcgcggagg ccgcccgaga gtaccgggcc gcatacgcct
      121 cgtggcgcgg gcaccgcgaa gagctggacc ggctctcggg ggagctggac gcccggcgac
      181 gggaagcgga gtttctgcgt gacgcgttgg aacgcatcgc cgacctcgac cccgcccccg
      241 gtgaggatgt tcgcctcgcg gagcgggccg aacgtctcac gaacctcgaa gagctgcact
      301 ccgccgcggc ggcggcccgt gaatggctct cgtcagaaga gggggagggc caggacgtcg
      361 tcagcctcgt ggccctggcg caatcgaccc tggcacgcgc cggcggccac gacccggcgc
      421 tcgccgcgct cgccgagacg ctcggctccc tcggctacgc ggcggccgac gcggccgccg
      481 agctctcccg ctacctcgac ggtctgcagc ccggtgcggc ggaagagctg gaggcgcttc
      541 acgagcgccg tgccgcgctt gccgcgctgc agcgctccta cgggcccggc ctcgacgacg
      601 tgctcaccct cgccgccgaa ggacccgggc gcctcgccct gctctcgggc gacgacgacg
      661 agatcgtcag gctgcgcggc ctcctgacgg ccgacgagga gacgcttggt cgcgcggccg
      721 cccggctgag cgcgcgtcgc gcggccgccg ccgaacgcct cggcgaggct gtcacccggg
      781 aactcgcggc gctcgcgatg cccgacgcac gcctcgtcgt cgaggtgacg gcgcttgcgg
      841 actacgccgc ccagggcaaa gatgcggtgc gcatgcttct tcgcccccac cggggcgccg
      901 aggcccggcc gctcggccga ggcgcctccg gcggtgagct ttcccgtgtc atgctcgcga
      961 tcgaggtggt catcgccgac gccgacccgg tgccgaccct cgtcttcgac gaggtcgacc
     1021 aaggcgtcgg cggcgccgtc gcgatcgaga tcggtcgacg cctggcgcgt ctggcccggc
     1081 acacccaggt catcgtcgtc acccacttgg cgcaggtcgc ggccttcgcc accaatcacc
     1141 tcgccatcgt caaggagcgc gaaggggctc tcacagcctc gagcgtgcgg cgcctcgacg
     1201 gcgcagaccg ggtcgccgag atgacgcggc ttctttccgg gctgaccggt tccgccaccg
     1261 gtgcccggca cgcgcaggag ctcctcgacc tcgccgccgc gtcctgaccc cacgccggtg
     1321 cgcaggagcc cggcagggcg cgggcgcccg cctctcatag ctccctcatc cggtaccgat
     1381 atcatcggca atcatgcgac gaagtcccgc ccgactgtat cgtcacgtca gacgcgccat
     1441 cggctacgag acgaacgcct tctggcgggc caagccggtc gagaacgaga ccgtcttcta
     1501 cgagtcgttc tccggcaacg gcatgctcgg caaccccgag gcgatctttc gccgtctcat
     1561 cgcgcgcggc gatactgcgg ggctgcacca tgtctgggcc ttgcggggcg cccggcacag
     1621 taaggcgcgg gcggagttcg ccggcgacga ccgcgtctcg ttcgtcgagt atcgttccgc
     1681 cgcgtactgg cgcgcattgt cgacgagcgg ctacctgatc aacaacgcca cattcccgcc
     1741 gcggttcggc aaacgcgacg gtcagaccta catcaacacg tggcacggca cgccgctgaa
     1801 acgcatgggc tacgacgtgg ccggcggcgc gctcgacacg gcgaacatcg tgcgcaactt
     1861 cgtgcaggcc gactatctgc tcgccgccaa cccgttcatg gcggagcaga tgtacgagag
     1921 cgcctacaag atgcgcggca tctttcgagg ccgcttcgtg gaggagggct atccgcgcat
     1981 cgaccggcag ttcctcgacc gcgcgggcgg gctcctggtg cgaggcgagc tgcgcgccgc
     2041 ggggctgccg gtcgccgacg acgacaagat cgtcctctac gcccccacct ggaaaggcac
     2101 gaagttcggt cggcccgaag acgacgtcga ggagttgacc gcgacggtgc ggcgcatcca
     2161 ggagcaggtc gcccccgccg gctatcgcgt gctcctgaag acccaccaga tcgtgcaccg
     2221 catgctgggc aaggcttcgg cccgggccca catgatcgtg ccgaacgaga tcgccaccaa
     2281 ccggctgctc ggcgtcacct ccgtcctcgt caccgactat tcgagcatct acttcgactt
     2341 tctcgccacc ggccggccca tcctcttcct cacccccgac atcgaagact atgaaggcta
     2401 ccggggcctg tatttcccgc ccgaggaatg gcccggcccc ctcgcccgca ccgccgacga
     2461 ggtcgccggg ttcgtgcacc gcatcgcggc ggacgggttg ggtgccgatg tcgaggcccg
     2521 ctatcgggcc gcccgtgacc ggttctgccc gcacgaagac ggcaaggtca ccgaccgcgt
     2581 catcgacgcc gtgttccggg gcgatcgggg gcggcgggtg cggccggccc gtgatggcgg
     2641 caagaccgcc gtgctcttct acgccggcgg catgcgttcc aacggcatca ccacctcgat
     2701 gctgaacctg ctcagccggc tcgactacga ccggctcgac gtctccgtct tcttccagaa
     2761 gtcgcgccgg gcggaggtca tcgccgggca gcgtgcgatg aatccgaacg tgcgccagtt
     2821 tccgcgcgtc ggcggcatga acggctccaa ggcggcgcag ctggtgcgcc acctcagcta
     2881 cggcgcgggc cggatcgcca ggcaccgcac cgacccgcat caacgcaggc tctgggacga
     2941 cgagtggcgg cgttgcttcg gcgcaagccg gttcgaccac gtcatcgact tcagcggcta
     3001 cagcccgttc tggtcgatcc tgctgctgca ttcgccggcc gcggttcgtt cggtgtggct
     3061 gcacaacgac atgctctccg acgcgcatcg cgacgtgcgg ggccgcaagc cgaacctgcg
     3121 cggcctcacc tccgagttct ccctttatcg ggagttcgac catctcgtct ccgtctcgcc
     3181 cgctttggcc gacatcaacc ggcaggccct ggcgcattac gccgcgccgg aggcgttcac
     3241 cgcggcggtc aacacggtcg acgccgccga cattcgccgc cggggtgccg aacggctgga
     3301 agacaccgtg cggcgggacg aagaagagca cctgcccgcg tgggcgatcg atctcatcgg
     3361 cggccccgcc cgcaccacgt tcgtgtgcgt cgggcgggtc tcgcccgaga agaaccacgc
     3421 ccgtctcgtg cgggcgttcg aggccgtgca tgcacggcac cccgacacgc ggctcgtgat
     3481 cgtcggcggc gggccgctcc agggcgagct cgaggcgcac gtctcccgcg tcgggctcga
     3541 agacagcgtc tacctgacgg ggcatcaggg caacccctac ccgatcgtcg cggcctccga
     3601 ctgtttcgtg ctttccagca actacgaggg tcagccgatg gttctgctcg aagccttggt
     3661 gctgggcgtg cccgccgtca cggtcaactt cggctccgca cgcgacgcgc tgccgcccgg
     3721 cctcggcctg gtcgtgcccg ccaccgacga ggggctcgag gcgggcatgg aggcgtacct
     3781 tcggggcgag gtaccgggcg gcgttttcga cgtcgccgcc tacaatcagg gtgcggtcga
     3841 gcagttctac cgggccgtcg gcctcacgga ggcgcatccg gctccccatg cggggcccgg
     3901 cctggcgcat ccagggccgg ggccgagtat tgataagata caagcccgtg gtgaatagtg
     3961 cgcagacgga gcccggcgga acgacaaaac acatcttcgt gacggggggc gtcgtttctt
     4021 ccctcggtaa gggactcacg gcggcgagcc tcggcaacct tctcacggca cgcggcctgc
     4081 gcgtcgtcat gcagaagctc gacccgtatc tcaacgtcga cccgggcacg atgaacccgt
     4141 tccagcacgg cgaggtcttc gtcaccgacg acggtgccga gacagacctc gacatcggcc
     4201 attacgagcg cttcctgaac gtgaacctca gcaaggccgc caacgtcaca acgggccagg
     4261 tctactcgca tgtgatcgcc aaggagcgcc gtggggagta cctcggcgac accgtgcagg
     4321 tgattccgca tatcaccgac gagatcaaga ggcgcatgcg cctgcaggcc tccgaggagc
     4381 cacgccccga cgtcatcatc accgaggtgg gcggcacgat cggcgacatc gaatcgcagc
     4441 cgttcatcga ggcggcgagg caagtgcggc acgagctcgg ccggggcaac tgcttcttcg
     4501 tgcacgtctc cctcctgccg ttcatcgcca cctccggcga gcagaagacc aagccgaccc
     4561 aacattccgt cgcggcgctg cgctcgctcg gcatccagcc cgacgcgctc gtcctccgca
     4621 gcgaccggcc ggtgtccgcc gccaatcggc gcaaggtcgc cctcatgtgc gacgtggagg
     4681 agaaggccgt cgtcaacacc gtcgacctgc cgagcatcta cgacatcccg gcgagcctgc
     4741 accgcgaggg cctcgacgac tacatcatcg acgcgctcgg cctcacagcg ggtgaggcgg
     4801 accgcagcga ctgggaggca ctgctcgagg tcgtgcacga cccgaagtac gaggtgacca
     4861 tcggcctcgt cggcaaatac gtcgacctgc ccgacgcata cctctccgtc accgaggccc
     4921 tgaaggccgg cgggttcgcg aacaacgcga aggtgcgcat cgtgtggatt ccctccgatg
     4981 agtgcgaggg gcccgagggg caggcgcgac acctctccgg ggtggacggc atctgcgtgc
     5041 cgggcggctt cggcatccgc ggcatcgagg gcaagctggg cgcgctgcgc ttcgcccggg
     5101 agaacggcct gcccgcgttg gggctctgtc tcggcctgca gtgcatggtc atcgaatacg
     5161 cccgcgacgt cgtcggcctg gccggcgcct cctcctcgga attcgacccg gagacgccgt
     5221 acccggtgat cgcgacgatg gcggagcagg tcgatattct gaacggcggc gacctcggcg
     5281 gcacgatgcg cctggggctt taccccgccg acctcgccgc cggttccgtc gtcgcagggc
     5341 tctacggggc ggcgcgggtc tcggaacgcc accgacaccg ctacgaggtg aacaacgcgt
     5401 tcaggaagcg tctcaccgag gccgggctgc gcttctccgg cacctcgcct gacggctcgc
     5461 tggtcgaatt cgtcgaattg cccgcgacga gccacccgtt ctacgtcggc acgcaggcgc
     5521 accccgagtt gcgttcccgg ccgaaccggc cgcacccgct ctttcgcggg ctgatcgagg
     5581 cggcgctcgc gcggcaggag gcctctcggc tcttcgaggt cgaagacgaa ctgggggcac
     5641 ccgttgactg agtctctcgc acacgacacg gcggccgccc ggccggccgg caccgccccg
     5701 gcggcagccg ccgcatccgc ctccgacctc gccgacagca ccgacccgag gcccgtcacc
     5761 cggagctcga tcgcctatca cggccgcatc tgggatgtcg tgaaagacga gttcccgtac
     5821 ggccaagcga ccctcacccg cgaatacgtc gcccacccgg gagctgtcgc gatcctcgcc
     5881 ctcgacgagg cggaacgggt gctcctcatc aaccagtatc ggcatcccgt gcgcagcatg
     5941 atgtgggaga tcccggccgg gctgctcgac gtcgacgggg agagccctct tgcggcggcc
     6001 cggcgagagc ttgccgaaga gaccgacctg gtcgcccgcg actgggccgt gctgggcgag
     6061 tactacacga ccccgggttc cagcagcgag gcgatccgca tctacctcgc ccgcggtatc
     6121 gccgccgctg aacgcccctt cgagcgcgag gcggaagagg ccgacattcg cctgcggtgg
     6181 gtgccgctcg aggccgccgt gaccgccgtt ctgggccggc gcatccagaa cccgtcggcg
     6241 gtcgtcggcc tgcttgccgc ctatgccgcg cggtcgagag gctggcagag cctcgcggaa
     6301 cccgatgcgc cctgggagcg ccacccgcgg tggcacgatg ggcgccttac ctcacgagac
     6361 gcccccgcgc ggccgtgaac ggcgtcgagg cggcggccga acagtacctg cgccacgcct
     6421 cgatcgaacg cggcctcgcc cgcaacaccg tgctggccta tcggcgagac ctcgccggct
     6481 atgccggctg gctgcgagaa cggggcgtca cccggccggc cgcggtgacg ccccgcgacg
     6541 tcgccacgtt caccgcgcac ctgcacgacg agggccgctc cgcggcgagc atcggccggg
     6601 cgctctccgc ggtgcggggc atgcatcgct tcctccgcga cgagggcgtc gttcccgatg
     6661 acgcgacccg cgagatccgc ccgccgaagc tgcccgcgag gctcccgcgg gtgctcagcg
     6721 tcgacgaggt cacccgcctc cttgccgcca ccgacgggga ccggccagag cggatccgcg
     6781 acaaggcgct cttggagctg ctctacgcca ccggggcgag gatcagcgag gccactcgtc
     6841 ttgacgtcga cgacgtcgtg ggcaaagacc ttgtgaggtt gcgtggcaag ggcgcgaagg
     6901 agcgcatcgt gccggtcggc tcctacgcgc aggcggcgct cgccgcctat ctcgtgcgcg
     6961 tgcgtccggc ctgggcggtg agaaaagcca ccccggcgct cttcctcggt gtacggggcg
     7021 gccggctctc ccggcagaat gcctggctca tcatccgtcg tgccgccggc atcgccgggc
     7081 tggagagcgc ggtctcgccg cacacgttgc ggcactcgtt cgccacccac cttctggagg
     7141 gcggcgccga cgtgcgcgtc gtgcaggatc tactcggcca ttcctccgtg acgacgacgc
     7201 agatctacac ccatgtcacc gccgacacgt tgcgcgacat gtacacgacg gcgcatccgc
     7261 gcgcccgttg aacggttgta gcgtgggcgg ggaaggagtg cgaatgagaa cattcaccat
     7321 gccgggcacc gacatcgtcg ccccgaacat catcctcggc cttatgagga tcgccgaaaa
     7381 gagcgacgaa gaggtacagg ccctggtgca cggggcccga gacaacggca tcgacttcgt
     7441 cgacatcgcc gacgtgtacg ggcccaccct gcacgcctgc gaagcgcgat tcgccgaggc
     7501 gatgcacctc accccgtcgg aacgcgacca ggtgacgatc cagacgaagg ccggcatcgt
     7561 tcccgaagag ggcgacgcgg cctacttcga cttctcgtac gaacacatca tggcctcggt
     7621 ggagggctcg ctgaaggccc tgcgcaccga ccggatcgac atcctgctct tgcatcgccc
     7681 cgacgccctt gtggagcccg acgaggtggc gcgcgccttc gacgagttgc acgccgacgg
     7741 caaggtgcgt ttcttcggcg tctcgaacca cacgccgcga cagatcgacc tcttgaagaa
     7801 gtcggtcgcg cagccactcg tcgccaacca gatgcagctc tccataacgc attcgccgct
     7861 catcgcccag ggcgtcgcag cgaacatgtc ggggctcgaa cagtcggtca ccctcgacgg
     7921 aggcggcctt ctcgactatt gcaggctgaa ccacatcacc gtgcaggcgt ggtcgccgtt
     7981 ccaggccgga ttcttcgacg gcgtcttcct cggctccgag cgtttccccg agctgaacgc
     8041 ggccatcgac cgcatcgccg ctgcgcatgg ggtgccgccc gcggcggtcg cgaccggatg
     8101 gatcacgagg catccggccg gcatgcaggt cgtgctcggc acgaccacgc cgtctcgcgt
     8161 ggccgattcg gcgcagggct ccgagctgcc actcacccgt tcggaatggt acgagctctt
     8221 ccgcacgtcg gggcaccgcg tgccgtgacc tcgcggcagg ccggcgtttc gatagactcg
     8281 acgcgcgccg gggtgacgcg gccgggcacg ggccggcctc gcaggcggct tgcgcgatcg
     8341 gccggcgcgg ttggccggcg tggtcggcac agggggagcg aggaggtcgt ccatggcgcg
     8401 gagtgacgac acccgaatcg agttgggcgg catcgacctg gccggccctg tcttggggcc
     8461 gacaggccgg ccgctgcgcg agttccccga accggcaccc ttgaccaccc acgggccggc
     8521 ccgcatcatc gcgatgtgca accagaaggg cggcgtcggc aagacgacga gcaccatcaa
     8581 cctgggtgcc acgatggccg acgacggccg ccgcgtgctc gcggtcgact tcgacccgca
     8641 aggtgcgctc tccgccgggc tcggggtcgc gacgcacgac gtgccgacgg tttacgacct
     8701 gctcgtctcg tcgcacaccg agcccggcga ggtgattcaa cacaccggca tcccgggcct
     8761 cgacctgctg ccggcgaaca tcgacctgtc ggccgccgag gtgcagctcg tgggcgaggt
     8821 cgcccgtgag cagatcctcg cccgcgtgtt gcgcaaggtc tctgccgact acgacctgat
     8881 ccttgtcgac tgccagccct ccctcggcat tctgaccgtc aacgccctga cggccgcgca
     8941 cggcgtcatc gtgccgctcg aatgcgagta tttcgccctg cgcggcgtcg ccctgttggt
     9001 cgagacgatc gagaaggtca aagaccgcct gaacccggtg ctcgagctcg atgggattct
     9061 cgccaccatg tacgacgcac gcaccctgca ctcccgcgag gtgctgcagc gcgtcgtcga
     9121 gaccttcggc gaccgggtgc tagagaccgt catctcccgc accgtcaagt tccccgacgc
     9181 ctccgtcgcg gccttgccca taacccgttt cgcacccgag catcaggcgg cggcggccta
     9241 ccgtcagctg gcgagggaat tgatcgcccg tggcgccgtc gcctgagctc gccgtacggc
     9301 ccggccgggc cttccgcgtc gatctgggcg gtttcgaggg gccgttcgac ctgctgcttt
     9361 ccctcatcgc caaacacgag atggatgtca ccgaagttgc cctcgcccgg gtggccgacg
     9421 agttcgtcga ctacctgcgc ggcctggaag aggcctccgg cggcgatgcg cggagcctcg
     9481 acgcggccag cggcttcctc gtggtcgcgg cgacgctgct cgacctgaaa gcgtcgcggc
     9541 tcctgccgca cgcggagccc gtcgacgcgg aagacgtcgc tgctttggag gcccgcgacc
     9601 tgctcttcgc caggctcctg cagtaccggg cgttccgtgg cgccgccgcc tggctggcgg
     9661 agcgtctcga cgaggagggc cggcgcgcgg cacgtgccgt gccgttggaa gagcggttcc
     9721 gaaagagcac cccggagctt cggtggcaga cgagcgtcga tgattttgct gtcgtcgcgg
     9781 ccctcgcgct cgcgcctgtc gccccgccgg tgatcccggt cgggcatctg cacgcgccgc
     9841 gggtcagcat ccgcaggcag gccgagcacg tggtgcgact gctccgttcc ggcgaggcac
     9901 acacctttca cgacctcatc gccgacgccg tgggggaggg ccgggccggg ctcgtcgtcg
     9961 cccggttcgt cgcggtgctg gaactgtacc ggcgtcgtgc gctctggctg cggcaagacg
    10021 acccgctcgg tgaccttctg gtgcgctggg tcgcgagcga ctgggacgag gcgagtctga
    10081 acgacttagg ggcgggctat gaatgacacg tcgctcgggg tgctgcccga gtcttcgccg
    10141 cgggaagagg cccgggacga gagcgaagcg gtctcctcac cggacgcccc cggcgcgggg
    10201 gcggccttgg cggcagacga tctgcggtgc cgggtggagg cgatcctcac cgtcgctgag
    10261 acgccggtcg agcccccggc gctcgccgcc gcgctgcagg tttcggccga cgaggcccag
    10321 gccgcgctcg acgcgcttcg cgcggactac gacggggagg gcccggggcc gcgccggggc
    10381 ttcgagttgc gccgcgtggg tggcggttgg cgcctttacg tgcgccgtga ctacgactta
    10441 gccgtcacgg ccttcgtctc cggccggcgt cgcgcccgcc tttcacaggc ggccctcgag
    10501 acgctcgccg tcatcgccta caagcagccc gtcacgcgtg gccaggtggc cgccatccgt
    10561 gccgtgaacg tcgattcggt ggtgcatacg ctcctcgcgc acggcctcat caccgagttg
    10621 ttcgccgacg aggagaccgg cgcgatcaac tacggcacca ccgatctgct cttgacgaag
    10681 ctcggcatcg actcccttga tcagctgccg aagatcgggc cgcttctcga cgacggcgcg
    10741 caggggttcg acgatgagct ccgctgacgg cgccgcgccc accggccccg gaaccgcggg
    10801 ggtccggctc cagaaagtgc tggctgcggc cggggtcgcc agccgccggg cgagcgagga
    10861 actcatcgcc gccggccggg tacgggtgaa cggtgccacc gtcacggagc agggccgccg
    10921 cgtcgacccc gcactcgatc gggtcgaggt cgatgggcgg cccgtgcagg tcgacgtcgg
    10981 caaacgttac gtgatcctca acaagccggt tggtgtggtc tccacgctgc acgacgaaga
    11041 cggcaggccc gacctcgcgg tgttcgccgg gcgctacccg gaacgcctct tcaacgtcgg
    11101 tcggctggat gccgccacct ccgggctgct gcttttgacg aacgatggcg agctcgcgca
    11161 cgtcttgatg catccctctt tcggggtcga gaagacctac gtcgcccggg tgcgcggcgc
    11221 cgtgacgccc cgcgtcgtcg cccgcctgac cgccggcatc gacctcgacg acgggccgat
    11281 cgccgccgac acggcgcgac ttttgcagac gggcggcggg gagagcctcg tggaggtcag
    11341 cctgcattcc ggccgcaacc gtatcgtgcg acgcatgttc gccgccgtcg ggcatccggt
    11401 gttggggctc gtgcggcgca gcttcggccc gctcgctctc accacgctgc cggcaggtca
    11461 gacacgcgag gcgacccggg cggaggtgag tgcccttctc gcgctcgccc gtgacgcgca
    11521 cgacgaaggg tcgcgcggcg gccgggggcg ccgcgacggg ggcgagccgg cgccgcggcg
    11581 tgcgcgaagg cccgcgtcgg gcgggtatcg tggccgcggc ggcaagaagc cgcagaggca
    11641 caggaattcg aggaggcccg gccgcaccgc ctgaacgcgg cgtcgcccgg cacgccggta
    11701 ggctcggcga cgggcgtgaa gccgcaggcg gcctcgaacg cgcggcccta cgccttgggc
    11761 ggctggctct gcggggcgct tcggccgtac ggaagaagct gaaagcagaa aggacggtcg
    11821 gtgtggcctc gacgacgaac gcgcggctga ccggcccggt gcgggttgtc ggcgcggggt
    11881 tgctcggcgc cagcatcggg ttggggttgc gctcgctggg cgtggatgtc gtgctcgccg
    11941 acgcctcgcc cgcggcgctg cgcctcgcgg tcgactacgg cgcgggccgt gccgccctgg
    12001 ccggcgaccg gccggccctc gtcgtcgtct gtgtgccgcc cgatcgcacc ggcgaggtcg
    12061 ttgccgccga gctcgtctcg tggcccgacg cagtcgtcgc cgacgttgcg agcgtgaagg
    12121 ctccggtgct gcgcgacatc gtctcgtgcc tcggtgcggc acacgcgggc gccctcatgc
    12181 ggtacgtcgg ctcgcatccg ctggccggca gggaacgcgg cggtccgatc gccgcccgcg
    12241 ccgacctctt tctcggccgg ccctgggtgg tctgcgcccc cgagggcacg gcgctagggc
    12301 cggtcggcgt ggtcgaagac ctgatcctcg acctcggcgc gacacccgtc actctcgacg
    12361 cggcgaccca cgacgaggcg gttgccgccg tctctcacgc gccgcagctg gtcgcctcac
    12421 tcatggcgaa gcagcttctc gagccgcccg ccgtcgcgtt ggggctcgcc gggcagggcg
    12481 tgcgagacgt cacccggatc gccgccagcg acccggaact gtgggtgcag atcctcgccg
    12541 cgaacgcccc cgccgtgggc cgcatcctcc aggcccttcg caccgacctc gaccgggtgc
    12601 tccaggcgct gggcgacccg gacgcgccgg gcgcccgccg ggcgctcgcg ggcaccctcg
    12661 cggccggcaa cacgggcgtc gcccggctgc ccggcaagca cggcctcgac cggcgctaca
    12721 cctccgtcgt cgtgctcgtc gacgacgtgc cgggcgaact cggcaggctc ttgaccgagc
    12781 tgggcgacat cggcgtcaac atggaagacc ttcggctgga gcattccccg gaggccgagg
    12841 tcgggctcgc cgagatctcg gtgctgccgc aggccgcgca gcaggccatc gatgagctga
    12901 ccgcacgagg ttggaggatc accgggtgaa cgacacgcag aaggtcggcg agcggatgcc
    12961 ggcgtcgggc ggtggcgggg ccggcgcccc ggtgttcgtc gtcgccatcg acgggccggc
    13021 cggcagcggt aagtcgagcg tcagcaaggc cgtcgccggc ctgctgggct tcgcctatct
    13081 cgacaccggc gccgtctacc gggcactcac ctggctggcg cttcgtcgcc gggtcgaacc
    13141 cggcgatcca gacgcgctcg cggccctcct ggcggcgttc cgcgacgtgc gcatcgacac
    13201 cgacccggcc gactaccggg tgcgggtcgc tcagacggat gtcaccgacg agatccgcac
    13261 cccggaggtc accggccggg tctccgcgct cgcccgcgtc cccgccgtgc gggagcatgt
    13321 gaatggcctc ttccggcgcc tcatcgccga aaccgcccgg cccggcatca tcgtggaagg
    13381 ccgcgacatc acgaccgtcg tcgcccccga cgccccggtg cgccttctcc ttaccgccga
    13441 cgccgcggtg cgcgccgccc gccgcgtcgg cgaactcgcc ggcgcggcgg gtcgggacgc
    13501 tgcgggggcg ctgcaccggc gggatgcggc cgactcgcgt gtcgtggact tcatgaacgc
    13561 ggcagacggc gtcgcgacgc tcgactcgac acgcctcgac ttcgcagcga ccgtcgccgc
    13621 ggtgcggcgc atcgtcgtcg acgcgcgggc ggccacccag gcacaagcgc gggccgtggc
    13681 ccgggcatca gctaaggccc aggctggcac gccgccacac gcaggtgcgg gaggggccgg
    13741 gcagtctgcc ggccgacggc ccggtgaggc gggcctcgag acgaacgggg acgattcatg
    13801 acggacggcg acgctccgat cgcccgcgac ggcgacccga acaggggggg cacccaaagc
    13861 ctcggcggca gccccgacac cgaggccggt gccgagcgca cccgcgccct gcgctcgggg
    13921 ctggatgcgt acgagctgga ggaatccgac ctcgccgtgt tagacggctc cccggcacag
    13981 gccgccgagg cggttccggt gctgccggcc gtcgcgattg tcggccggcc gaacgtcggc
    14041 aagtccgcac tcgtgaaccg tatcctcggc cgccgcgagg ccgtcgtcga agaca
//
LOCUS       DASTNL010000031         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1137281, whole genome shotgun sequence.
ACCESSION   DASTNL010000031 DASTNL010000000
VERSION     DASTNL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137281"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..383
                     /locus_tag="VFQ96_00885"
     CDS             141..383
                     /locus_tag="VFQ96_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256374.1"
                     /translation="MVPEMPEDGKKETPTTVRRRVLTAIVAACRSGAETTLRYALGWP
                     PSVGEWKALAELVERGLIEVGGRGIHVTAAGTTALA"
     gene            complement(411..1379)
                     /locus_tag="VFQ96_00890"
     CDS             complement(411..1379)
                     /locus_tag="VFQ96_00890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEU0256375.1"
                     /translation="MGLRGTAFFVATTAGLWALRRLSAGPGGTDVATASGEPRQAVAH
                     TVEPAGRARSTTSAGPADGEMRPWRELREHGLRVERGLRLFGSGVSRIMVEFEPPVQI
                     NNATMHVYGGFRIGAYSFLRTGTMRHVTSIGRYTSIGPGVILGEGEHPIEWLSLSPTV
                     FDRNRWFFHPESKRLAPTIVRRRTRENAPSTPTGKVRIGNDVWIGANAIIRRGVTIGD
                     GAIVAAGAFVNRDVPPYTIVGGLPARKIRDRFDPGLVKRLLAFRWWEFDARDLAGVPF
                     ERPGEALDEIARRERAGRARRTPPHHDTVILTQAGWTDLRHRHVTR"
     gene            complement(1453..3405)
                     /locus_tag="VFQ96_00895"
     CDS             complement(1453..3405)
                     /locus_tag="VFQ96_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEU0256376.1"
                     /translation="MTGVLSLTERLRSLGDEELTTLIRRRGVEPGGPADFFELAEVLL
                     GRRSIERALRHADRRCLAVLAIAAGPGTPVTRKQVVDALRVNGAEWADDGPALDTALA
                     WALDEALAVAVGDALSCLPPVAEQLHDWPTHGLPGVAELCAAPTPVETGAPREGGDGG
                     EDPDRRDAGVAFDGVGLVADLLLRLQQQSPPAPTHAGRLTASSLRRTADEIGAPAPLA
                     RTLFRLAARARLVAAEGAEARVTHAGHEWLREGPALRWSQLAGRWVKGLSGRVRTVLG
                     ATSAWGPHAAAHARWLYPAAGSGLVKRLAAAEEEAHSLALAADGRRSPTGGRLFLAGA
                     DVAAAALAPHFPSPVTSVYVQDDLTIIAPGPLRNDLDARLRDFCEAEGGRLASSYRVT
                     EQSVTRAAAEGLRAAEILDFLAGISTTGIPQPLEYLVTEAAERYGRIRVTDMVEGRDR
                     PVIASPRGPARTHERRNDPALAAGAAATRGHARSRVSSEDETLLRAVEVDSALRDLAL
                     RRDGDILISNQEPVEVLRRLVAARYPAAGEPGRGIATAAWPDDDGHAAEGSEDSAAAR
                     LVARLRQAETLAGPELHRAWIGRALEAAAHTRSEVGLEVQMPGGAVRGIRLKLTGFSA
                     TRVRGRDVAADVERTLPLSSITAVLP"
     gene            complement(3475..3747)
                     /locus_tag="VFQ96_00900"
     CDS             complement(3475..3747)
                     /locus_tag="VFQ96_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256377.1"
                     /translation="MMAQDPRTDSPLQRVLAYATVICIVLSLAAITAIIVAYSMGIAQ
                     SDQLRHGLWPAIVATPMVGFPLGLILMMVLLWTTSLARRARSRAES"
     gene            3798..4178
                     /locus_tag="VFQ96_00905"
     CDS             3798..4178
                     /locus_tag="VFQ96_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU0256378.1"
                     /translation="MPTGKVKFYDEEKGFGFITSDDGHEVFLHASAIPAGAVVRAGSR
                     VEFGVVDGKRGAQALSVRVLKAPPSMVRRSRRSADDMAVVVEDLVKLLDGIGASLRRG
                     KYPERSHGRKVAALLRRVADDLDA"
     gene            4171..4755
                     /locus_tag="VFQ96_00910"
     CDS             4171..4755
                     /locus_tag="VFQ96_00910"
                     /inference="COORDINATES: protein motif:HMM:NF022674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEU0256379.1"
                     /translation="MRDEAAVAPFTVGAREREVAMAALREITDVTTIGAFRGTETRGD
                     GVAVWFGCTQPAYPDWRWEVVLAKPLGQDQATVVEAGLLPTDGSLLAPDWVPWSERLA
                     EYRAGQEAALQAGGGSAGPSAGDDEVEASAFAADDALAGDDGEDDDEGDDGDDDPDED
                     DPGEIEIDERTDDLIDELDPEGAGPGDDEGRDER"
     gene            complement(4752..>4937)
                     /locus_tag="VFQ96_00915"
     CDS             complement(4752..>4937)
                     /locus_tag="VFQ96_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEU0256380.1"
                     /translation="DLDEHVQAAELSRQLRANGVVDIDSYRKLNRNQLRIGTFASVEP
                     SDVRALTACLDYVIERL"
BASE COUNT          705 a         1685 c         1813 g          734 t
ORIGIN      
        1 accaggccgc cggccacgac caggccgggg gagtcgttcc ttcgcccggg cgtgtggtgt
       61 gcgcggccca cccgcggttc ccggcgattt cctggccgat gcctcgcgca cgacaaggcg
      121 gggcgggtta gcgtgtgggc atggttccgg agatgccgga agacggcaag aaggagacgc
      181 ccacgacggt acggcgccgg gtgctgacgg cgatcgtcgc cgcgtgccgc agtggcgcgg
      241 agacgacgtt gcgctacgcc ctcggttggc cgccgagcgt gggggaatgg aaggccctgg
      301 ccgagctggt cgagaggggt ctgatcgagg tcggtggccg cggcatccat gtgacagccg
      361 cgggcaccac ggcgctcgcc tgatgcttct gacggctcgc ggcgcgtccc ttaccgtgtg
      421 acgtggcggt gccgaaggtc ggtccagccg gcctgggtga ggatgacggt gtcgtggtgc
      481 ggcggcgtgc ggcgggcgcg accggcccgc tcgcggcggg cgatctcatc cagggcctcg
      541 ccgggccgct cgaagggcac gccggccagg tcgcgggcgt cgaactccca ccagcggaac
      601 gcgaggaggc gcttcaccag ccccggatcg aagcggtctc tgatcttgcg ggcgggcaac
      661 cctccgacga tcgtgtaagg cggcacgtcg cggttcacga acgcgccggc cgccacgatg
      721 gcgccgtcgc cgatcgtcac gccgcgtcgt atgatcgcgt tcgcgccgat ccacacgtcg
      781 ttgccgatcc gcaccttgcc ggtcggcgtg gaaggcgcgt tttcccgggt gcggcgccgc
      841 acgattgtgg gggccagccg cttgctttcc gggtgaaaga accatcgatt ccggtcgaac
      901 acggtggggg agagcgacag ccattcgatc ggatgctcgc cctcgccgag gatcacgccc
      961 gggccgatgc tcgtgtagcg gccgatcgac gtcacgtgcc gcatggtgcc ggtacgcaag
     1021 aacgagtatg cgccgatgcg gaacccgccg tagacgtgca tcgtcgcgtt gttgatctgc
     1081 acgggcggct cgaactccac catgatgcgg ctcacgccgg agccgaagag ccgcaagccc
     1141 cgttcgaccc gcaggccgtg ttcccgtagt tctcgccatg gccgcatctc gccgtcggca
     1201 ggccccgcac tggtggtgga acgcgctcgg ccggcgggtt cgaccgtgtg cgccacagcc
     1261 tgtcgtggtt ctccactcgc cgtggcgaca tcggtgccgc ccgggccggc gctcaggcgg
     1321 cgaagcgccc agagacccgc cgtcgtcgcg acgaagaacg ccgtccctcg aagacccaca
     1381 gcaaccacct ccggcagggt cagattttca caggccgcgg gcccccgtca aatgccggcc
     1441 gggcgcggaa ggtcagggca ggaccgccgt gatgctcgaa agcggcagcg tgcgctccac
     1501 gtcggcggcg acgtcgcgcc cgcgcaccct ggtggcgctg aacccggtga gcttcaaacg
     1561 gatgccccgt accgcgccgc cgggcatctg cacctccagc cccacttcgc tgcgagtgtg
     1621 cgctgcggcc tccagcgccc ggcctatcca ggctcgatgc agctcgggcc ccgccagagt
     1681 ctccgcctgc cgaagccgcg ccaccaaccg ggcggcggcg ctgtcttcgg agccttctgc
     1741 cgcatgcccg tcgtcgtcgg gccaggccgc cgtggcgatg ccgcgcccgg gctctccggc
     1801 ggccgggtat cgggccgcga cgaggcggcg cagcacctcg accggctcct ggttgcttat
     1861 aaggatgtcg ccgtcgcggc gcagcgcgag gtcgcgcaac gcgctgtcga cctcgaccgc
     1921 gcgcagcagg gtctcgtctt cgctcgacac gcggctgcgg gcgtggcccc gcgtcgccgc
     1981 ggcgccggcg gccagcgccg ggtcgttcct tctctcgtgc gttcgcgccg ggcctcgcgg
     2041 cgatgcgatg accggccggt cgcggccctc gaccatgtct gtcacgcgga tgcggccgta
     2101 gcgttctgca gcctccgtca cgagatattc cagcggctgc ggtatgccgg tcgtcgagat
     2161 tccggcgaga aagtcgagaa tctcggctgc gcgcaagccc tcggccgccg cgcgcgtgac
     2221 cgactgttcg gtgactcggt aagaggaggc cagccggccc ccctcagcct cgcagaagtc
     2281 tcgcaggcgg gcgtcgagat cgttgcgcaa cgggcccggg gcgatgatgg tcaggtcgtc
     2341 ttgtacatag accgaggtga ctggcgacgg gaaatgcggc gcgagagccg ccgcggcgac
     2401 gtctgcgccg gccaggaaaa gtcggccgcc ggtggggctc cggcgcccgt ccgccgcgag
     2461 ggcgagactg tgggcctcct cttcggcggc ggcgagcctc ttcaccaggc cgcttccggc
     2521 cgcggggtag agccatcggg cgtgtgccgc cgcgtgaggg ccccaggcgc tcgtcgcccc
     2581 gaggaccgtg cgtacccggc cggagaggcc cttgacccat cgaccggcaa gctgtgacca
     2641 gcgcagcgcg gggccctcgc ggagccactc gtggcccgcg tgcgtcaccc gtgcctcggc
     2701 cccttctgcg gcgacgaggc gcgcgcgcgc ggccagccgg aagagggtgc gagccagggg
     2761 ggcgggggcg ccgatctcgt cggccgtgcg tcgcaggctc gacgcggtga gccggccggc
     2821 atgggtgggt gcgggcggtg actgctgctg aagccggaga aggaggtcgg cgacgagccc
     2881 gacaccgtcg aacgccacgc ccgcgtcacg gcggtcgggg tcttcaccgc cgtcgccccc
     2941 ttctctcggg gcgcctgtct cgaccggcgt gggcgcggcg cagagctccg ccacaccggg
     3001 caggccgtgg gtcggccagt catgcagctg ttcggcgacg ggcggaaggc acgagagcgc
     3061 atctcccacg gccacggcaa gggcttcgtc gagcgcccag gcgagggcgg tgtcgagcgc
     3121 aggtccgtca tcggcccact cggcgccatt cacgcgcagt gcgtcgacga cctgcttcct
     3181 ggtcacgggg gtgcccggcc ccgccgcgat cgccagaacc gccaggcacc tgcggtcggc
     3241 gtgccggagg gcgcgctcga tactgcgtcg gccgaggaga acctcggcca actcgaagaa
     3301 atctgcgggg ccgccgggct ccacacctcg ccgcctgatc agtgtcgtga gttcctcgtc
     3361 gccgaggctc cgcagccgtt ccgtgaggct caagacaccg gtcaccgacg cgtcttcacc
     3421 gttcccttcg gtctccgttc cgtcttctcc gtctttgccg ccatggtcag gccctcacga
     3481 ttccgcgcgc gagcgcgctc ggcgggccag cgacgtggtc cagagcagca ccatcatgag
     3541 gatgaggccg agcgggaagc cgaccatcgg tgtggcgacg atcgccggcc agaggccgtg
     3601 gcgcaactgg tcgctctgcg cgatccccat cgagtaggcg acgatgatcg ccgtgatggc
     3661 ggcgagggag agcacgatgc agatgaccgt cgcgtaagcg agcacccgct gcaggggaga
     3721 atcagttcgc ggatcctgtg ccatcacgca atcaaggata attgacccgg acgcggtttc
     3781 ggatgaaaga ggtttccatg cccaccggca aggtcaagtt ctacgacgag gagaaggggt
     3841 tcggcttcat cacctccgat gacggccacg aggtcttctt gcacgcctcg gcgattccgg
     3901 caggggcggt ggtgcgcgcg ggcagccggg tcgagtttgg cgtcgtcgac ggcaagcgtg
     3961 gcgcccaggc cctctcggta cgcgtgttga aggccccgcc cagcatggtg cgccgctccc
     4021 gtcgttctgc cgacgacatg gctgtcgtcg tcgaagacct cgtgaagctt ctggacggca
     4081 tcggcgcgag cttgcgccgc ggcaagtatc cggaacgttc gcacggccgc aaggtggccg
     4141 cactgttgcg tcgtgtggcg gatgatctcg atgcgtgacg aagctgccgt ggcgcctttc
     4201 acggtcggcg cccgcgagag agaggtcgcc atggcggccc tgcgagagat caccgacgtc
     4261 acgacgatcg gcgccttccg cggcaccgag acgcggggcg atggggtcgc cgtgtggttc
     4321 ggctgcaccc agccggcgta ccccgactgg cggtgggagg tcgtgctcgc gaaaccgctc
     4381 gggcaagatc aggcgacggt cgtcgaggcg ggcctcctgc ccaccgacgg ctctcttctc
     4441 gctcccgact gggtgccgtg gtcggagcgg ttggccgagt atcgcgccgg ccaagaggcg
     4501 gcgctccagg ccggcggggg ctcggccggg ccttcggcag gggacgacga ggtcgaagcc
     4561 tccgcgttcg ccgccgacga cgcgttggcg ggcgacgacg gtgaggacga cgacgaaggg
     4621 gacgacggtg acgacgaccc tgacgaagac gacccgggcg agatcgagat tgacgagcgg
     4681 accgacgacc ttatcgacga actcgacccg gagggtgccg ggccaggcga cgacgagggc
     4741 cgtgacgagc gctaaagcct ctcgatgacg tagtcgaggc acgcggtgag agcgcgcaca
     4801 tcgctcggct cgacggaggc gaaggtgccg attcgcagct ggtttcggtt cagcttgcgg
     4861 tacgaatcga tatcgacgac gccgttcgcc cgcagctgac gggagagttc ggcggcctga
     4921 acgtgctcgt cgaggtc
//
LOCUS       DASTNL010000032         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_580887, whole genome shotgun sequence.
ACCESSION   DASTNL010000032 DASTNL010000000
VERSION     DASTNL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580887"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1931
                     /locus_tag="VFQ96_00920"
     CDS             201..1931
                     /locus_tag="VFQ96_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0256381.1"
                     /translation="MEQQTPARALSATSRDDAEHRLRALLRAGRAVAQPLDLATVLHR
                     IVEAAVDLVESRYGALGVYTHDGTLETFIQVGLTQHEVEGLGPLPTAHRGVVGALTNS
                     STPIRLRHISDDPRSVGTPPGHPAVDSFLGVPILVRGRPYGNLYLINRVTGEFTQEDE
                     DSVVALAATAGSAIENARLFARAQREREWASAAADATAAALSTDSDPLVPVAVCLQRL
                     CDADLVACIGPRGDVNVLAGTADDAPVPQDALVTAWPAAAKAATAPVTTEEGEAPARP
                     TAASWPSAEPGGRSRPVLLLPVPGPASEGRTIAVVRPSSGPAFTGADLDSAADFAARV
                     GVTLQLAKAHADEERVRLMADRERIARDLHDQVIQQIFAAGLNLQALATTADAETARR
                     LTGTVTALDGAIAQIRSAIFALSDTDAGAARKTTRSAVLDVVDTLADALPRTPNIEFA
                     GPVDHTVTGALEDDVVAVVREGLTNIVKHAAAAHISLAVRVSPEGRVTVDIDDDGVGR
                     VKVKPHGGLDNIRSRAKRHGGSLQIASGAGGTRLHWSVPHLAPRDATAHRDRSESQPE
                     RGRPGKARRR"
     gene            1928..2584
                     /locus_tag="VFQ96_00925"
     CDS             1928..2584
                     /locus_tag="VFQ96_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0256382.1"
                     /translation="MIRVLVVDDHELVRHGIVTLLETAPDVEVVAEAAGATEAVARAR
                     ATKPDVAVLDMRLPDGNGVDLCRHIRSEREATHCIILTAYDDDQAMEAAVLAGASDYV
                     LKDIQASGLLERIRSAAAGRSRLGVPLRRQVADTMSRRRAADPRLGALSTRERQVLAL
                     VAEGMTNREIGIRLSLAEKTVKNYVSNLLAKLGVEHRTQAAVLHLQAGRGEAHPAAEP
                     "
     gene            2823..3113
                     /locus_tag="VFQ96_00930"
     CDS             2823..3113
                     /locus_tag="VFQ96_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256383.1"
                     /translation="MPVYTRRRHNVTAYRWAAREHGSHPAFVSAWETAVEEDKIHPSC
                     GRPWREHAVTTDEHHQIVCPGQWAVNEIDGTVRVLDDHDFHDDYEAPEELAS"
BASE COUNT          514 a         1203 c         1122 g          383 t
ORIGIN      
        1 gttgactccg gggcctgagc tgcaggtgcg caccacggca cgaccgacac cgcgcgtcgg
       61 ccaggcgtgc cgggtcgaga tcgagcctgg cgacgtcacc gtctggttgg cacgagcggc
      121 ggccggcggg gccgacgaga ccgcacggcc ggagccatcc cgcagcgaag gctgatagaa
      181 agtcgggagg ggggaggctc atggaacagc agactccggc aagagccctc tcggccacga
      241 gccgcgacga cgcagagcat cggctgcgcg cgctgttgcg tgcgggccgt gccgtcgcgc
      301 agccgctgga tctcgcgacg gttctgcacc gcatcgtcga ggcggccgtc gacctcgtcg
      361 agtcccgcta tggcgccctg ggcgtctaca cgcacgacgg caccctggag acgttcattc
      421 aggtggggct gacgcagcac gaggtggagg ggctcgggcc gctgccgacg gcgcaccgcg
      481 gcgtcgtggg cgccctgacc aacagcagca cccccatccg gctgcgccac atctccgacg
      541 acccacggtc ggtgggcacc cctcccggcc acccggcggt cgacagcttt ctcggcgtgc
      601 cgattctcgt gcgcggccgc ccctacggca atctctacct gatcaaccgg gtcaccggcg
      661 agttcacgca agaagacgaa gattccgtcg tcgcgctcgc ggcgacggcg ggctcggcca
      721 tcgagaacgc ccgcctcttc gccagggcgc agcgcgagcg ggaatgggcg agcgcggcgg
      781 ccgacgcgac ggccgcggcc ctctccacag actccgaccc gctggtcccc gtcgccgtgt
      841 gcctgcagcg cttgtgcgac gccgacctgg tcgcctgtat cggcccgcgc ggcgacgtga
      901 acgttttggc cggcaccgcc gacgacgccc ccgtgccgca agacgcgctc gtgacggcgt
      961 ggcccgcggc ggcgaaggcc gcgacggcac ccgtgacgac cgaagaaggt gaggcgcccg
     1021 ctcggccgac ggccgcgtcg tggccgtcgg ccgagcccgg cggccggagc cggccggtgc
     1081 tgctcctgcc cgtgccgggc ccggcatccg aaggccgcac gatcgcggtc gtgcgcccct
     1141 cgagtgggcc cgcgttcacc ggcgcggatc tagattccgc cgcagacttc gcggcacgcg
     1201 tcggcgtcac cctgcagctg gcgaaggcgc acgccgacga ggagcgggtg cgcctgatgg
     1261 ccgaccggga acggatcgcc cgcgacctgc acgaccaggt cattcagcag atcttcgcgg
     1321 cgggcttgaa cctgcaggcc cttgcgacca ccgcagatgc cgagaccgcg cggcggctga
     1381 cgggcaccgt cacggcgctc gacggcgcca tcgcgcagat ccgctcggcg atcttcgccc
     1441 tctccgacac ggatgcgggc gcggcacgca aaaccacgcg aagcgccgtg ctcgacgtcg
     1501 tcgacacgct cgccgatgcg ctgccgagga cccccaacat cgagttcgcc ggccccgtcg
     1561 accacaccgt cacgggcgct ctcgaagacg acgtcgtcgc ggtggtgcgc gaagggctca
     1621 cgaacatcgt gaaacacgcg gccgccgccc acatctccct cgccgtgcgg gtgagcccgg
     1681 agggccgggt caccgtcgac atcgacgacg acggagtggg gcgggtgaag gtgaagcccc
     1741 acggcgggct cgacaacatc cgttcccgcg cgaagcggca cggcgggagc ctgcagatcg
     1801 cctccggcgc cggcggaacg cgcctgcact ggagcgtgcc ccacctggca ccccgcgacg
     1861 ccaccgccca ccgcgaccgc agcgagagcc agccggaacg tggccgacca ggcaaggccc
     1921 ggcggcgatg atccgggttc tcgtcgtcga cgaccacgag ctcgtgcggc acggcatcgt
     1981 gacgctgctg gagaccgcgc ccgacgtcga ggtcgtcgcc gaggccgccg gcgccaccga
     2041 ggcggtcgcc cgcgcccgtg cgacgaagcc cgacgtggca gtgctcgaca tgcggctgcc
     2101 cgacggcaac ggcgtggatc tctgccgaca catccgctcc gaacgcgagg cgacgcactg
     2161 catcatcctg accgcctacg acgacgatca ggcgatggag gccgccgtct tggccggcgc
     2221 ctccgactac gtcttgaaag acatccaggc ctccggcctg ctggagcgca tccgttccgc
     2281 cgcggccggc cgcagccggc tgggcgtgcc tctcagacga caggtcgccg acaccatgag
     2341 ccggcgccgc gcggccgatc cacggctcgg cgctctcagc acccgtgagc gccaagtgct
     2401 cgccctggtg gcggagggca tgacgaaccg cgagatcgga atacggcttt ccctcgcaga
     2461 gaagacggtc aagaactacg tctccaacct gctggcgaag ctcggcgtgg agcatcgcac
     2521 gcaggcggcg gtgctgcacc tgcaggccgg ccgcggcgag gcgcaccccg ccgcggagcc
     2581 atgagacacc gcctcgcacc gtgctaaaag cggtttggga ctttcggcac tattaccgcc
     2641 atgcccgcgg gccggatgct gatgtcatga gcgtcagagc ctcggcaccc gccacgggcg
     2701 gccacgcctc acgcccatct cgccggcggc gccccgaacg agcccggccg gcaccatgcc
     2761 cgggagaagg ccgaaccccg gccgagaagg cccccggcga cacgagaaag gaaccagccg
     2821 acatgcccgt ctacacgaga aggcgtcaca acgtcaccgc gtaccgctgg gccgcgcgag
     2881 agcacggcag ccatccagcc ttcgtctcgg cgtgggagac cgccgtcgaa gaggacaaga
     2941 tccacccctc ctgcgggcgg ccctggcgcg agcacgcggt gacgacggat gagcaccacc
     3001 agatcgtctg ccccggccaa tgggcggtca acgagatcga cggcaccgtt cgcgtgctcg
     3061 acgaccacga cttccacgac gactacgagg cgcccgaaga gctcgcctcc taaccggccg
     3121 ggccggtgcg gcccccggtg caaagccgac gcctggcagg cgacggcggc gacggcgaca
     3181 tgccttggcg cgggggttcg tcgcgcgcca aggcacggca cg
//
LOCUS       DASTNL010000033         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_951925, whole genome shotgun sequence.
ACCESSION   DASTNL010000033 DASTNL010000000
VERSION     DASTNL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951925"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1189
                     /gene="nusA"
                     /locus_tag="VFQ96_00935"
     CDS             194..1189
                     /gene="nusA"
                     /locus_tag="VFQ96_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU0256384.1"
                     /translation="MDIDLGVLRLLEREREIPFDELAGIIEQAILTAYLKHTGQERDH
                     ELPGAPARVALDRKTGHVTIYVPERDESGAVIGEAVDDPHDFGRIAAFAAKQVINQRL
                     RDIQDEAVLGEFHGKEGDIVAGVVQQGPNPRMIHVDLGSVEAILPPEEQVPGEDYAHG
                     TRLRVYVTSVSRGAKGPSITVSRTHPGLVRKLFALEVPEIASGAVVIESLAREAGHRT
                     KIAVRATEPGINAKGACIGELGRRVRAVTAELNDEKIDIVDYSPDLATFVASALSPAK
                     VTSAFVIDERSRAVRALVPDYQLSLAIGKEGQNARLAAKLTGAKIDIQPDSILDD"
     gene            complement(1403..2416)
                     /gene="fgd"
                     /locus_tag="VFQ96_00940"
     CDS             complement(1403..2416)
                     /gene="fgd"
                     /locus_tag="VFQ96_00940"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HEU0256385.1"
                     /translation="MLRFGYKASAEQFGPEDLLRYSVLAEKVGFDSVFTSDHLQPWRH
                     DGGHAPAALPWLGALGASTRRITIGTSVLTPTFRYHPAVIAQAFATLGRLFPGRVILG
                     IGSGESMNEAPLNIAWPAPKERFARLKEAIELIRRLWVEERVDFDGAYYSTHHATIYD
                     RPERPVPLYIAGSGPSTTRLAGRLGDGFITTSGKAPELYSETLLPAVRQGIARSGRNP
                     ADFDMLLEVKVSFDHDAERALADTRFWGALALKPEEKTGIEDPIEMQRLADQLPIERA
                     ASRFIVSSDPDEHVAAIARYLDLGFTHLIFHAPGPDQERFLRLYGAEVLPRLRALSRS
                     GTR"
     gene            2663..>2949
                     /locus_tag="VFQ96_00945"
     CDS             2663..>2949
                     /locus_tag="VFQ96_00945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0256386.1"
                     /translation="MHLVIARYAVKAGMTEEVVDLLAGLTAASRREEGNLSYNFYRVE
                     GEDAIVLVERYSSAEAFAAHCESEHFRTILLGEVVPRLESREVTAVPATEY"
BASE COUNT          472 a          977 c         1041 g          459 t
ORIGIN      
        1 gccgccgcgc ggccgaaggg atcgtcgagg tgtggaaccg gcgcaccggc gagaggcgcg
       61 aggtcgcgcg ctcgcaggtc gtcgccgagg tgcgttcgaa ggcctgagac cgaccgcctg
      121 cacgcctggt cgggtaccgt agagtccacg ctgcctggtc ggcagcaggc gtcataaaag
      181 aatagggggc ggaatggata tcgacctcgg ggtgctgcgc ctcctggagc gtgagcgtga
      241 gatcccgttc gacgagttgg ccgggatcat cgagcaggcg atcctcaccg cctatctgaa
      301 gcacaccggc caggagcgcg accatgagct gccgggcgcc cccgcccgcg tcgccctcga
      361 ccgtaagacg gggcacgtga ccatctacgt gccggagcgt gacgagtccg gcgcggtcat
      421 cggcgaagcc gtcgatgacc cacacgactt cggtcgtatc gccgcattcg ccgcgaagca
      481 ggtgatcaac cagcgcctgc gagacatcca agacgaagcc gtgcttggcg agttccacgg
      541 caaggagggc gacatcgtcg ccggtgtcgt gcagcaaggc cccaacccgc gcatgatcca
      601 cgtggatctc ggctctgtgg aggcgatcct gccgccggaa gagcaggtgc cgggtgagga
      661 ttacgcccac ggcacgcgcc tgcgcgtcta cgtcacgagc gtctcccgcg gcgcgaaagg
      721 gccgtcgatc accgtctcgc gcacccaccc cggcctcgtg cgcaagctct tcgccctcga
      781 ggtgccggag atcgcctccg gggcggttgt gatcgaatca ctcgcccgcg aggcgggtca
      841 tcgcacgaag atcgcggtgc gcgccaccga gccgggcatc aacgcgaagg gtgcgtgcat
      901 cggcgagctc ggccgtcggg tgcgcgcggt caccgcggag ctcaacgacg agaagatcga
      961 catcgtcgac tattcgcccg acctcgcgac tttcgtcgcg agcgcgctct cgcccgccaa
     1021 ggtgacgagc gcctttgtca tcgacgagcg ttcccgcgcg gtgcgtgccc tcgtacccga
     1081 ctaccagctt tcgctggcga tcggcaagga ggggcagaac gcgaggctcg cggcaaagct
     1141 cacgggcgcg aagatcgaca ttcagccaga cagcatcctc gacgactgac acggcacccg
     1201 tgaggccggc cgttcgccga gagggccggc ccgacagccg tcggccgggt gcccgcgtcg
     1261 ccggggtgtg acgcgaggca tgccgggtgc cgctgggacc cgcacgaccc cggggtcggc
     1321 tccaccgacc cctccgtcgt gctgaggccg gcatcgagaa cctcgcggag gcctggccta
     1381 cggcccctat ggcgcccccg gttcagcgag tgccgctgcg ggaaagggcc cggagcctcg
     1441 gcaggacctc cgcgccgtag aggcgcagga agcgttcctg gtcggggccc ggcgcgtgga
     1501 agatcaggtg ggtgaaaccg aggtcgagat atcgggcgat cgccgcgacg tgctcgtcgg
     1561 ggtcgctgga gacgatgaat cggctggcag cccgctcgat cggcaactgg tcggcgagcc
     1621 gctgcatctc gatcggatcc tcgatgccgg tcttctcctc cggcttcagg gccagcgccc
     1681 cccagaagcg cgtgtcggcg agggcccgtt cggcatcgtg gtcgaacgac accttgacct
     1741 cgagaagcat gtcgaagtcg gcggggtttc ggcccgaccg cgcgatgcct tggcgcacgg
     1801 cgggcagcag ggtttcggag taaagctcgg gggccttgcc gctcgtcgtg atgaatccgt
     1861 cacctagccg gccggccaga cgcgtcgtcg aggggccgga acccgcgatg tagagcggca
     1921 cgggtctttc cgggcggtcg tagatggtgg cgtggtgcgt cgaatagtag gcgccgtcga
     1981 agtcgactcg ttcttcgacc cacagccggc gtatcagttc gatcgcctcc ttcagccgcg
     2041 cgaaacgctc cttcggtgcg ggccaggcga tgttcagcgg ggcctcgttc atcgactcgc
     2101 cggagccgat gccgaggatg accctgccgg ggaaaagccg cccgagggtg gcgaacgcct
     2161 gggcgatgac ggccgggtgg taccggaagg tgggggtcaa aacactcgtg ccgatcgtga
     2221 tgcgccgggt ggaggcgcct agcgcgccca gccacggcag ggcggccggc gcgtggccgc
     2281 cgtcgtggcg ccacggctgc aggtggtcgc tcgtgaagac ggagtcgaag cccaccttct
     2341 cggccagcac cgagtatcgc aagaggtctt cggggccgaa ctgctccgcc gaggccttat
     2401 acccgaacct gagcatcgca accctgtcct ctcagaattc ctcgtcggtc gcctgtcgcc
     2461 gcccgtgccc gcccggttga cgagaagcac ggctcctgcc gccgcccgtg ggccgaaggg
     2521 tcgggagcgc cagccttgcg gcacgcacat ttgtcgtaac tgtggaaccc gcgattcgat
     2581 tccctttccg ccggagcgtc ggcgtctcgc cggtgccccg tcgtagggtg gtatgtcgcg
     2641 gtcacgcggg gaaggaagag ccatgcatct cgtcatcgct cggtacgccg tcaaggcggg
     2701 gatgaccgag gaggtcgtcg acttgctcgc cggtctcacc gccgcaagcc gacgtgagga
     2761 gggcaacctt agctacaact tctatcgcgt cgaaggtgag gacgcgatcg tcctcgtcga
     2821 gaggtattcc tccgccgagg ccttcgcggc gcattgcgag agcgagcact ttcgcacgat
     2881 tctgctcggc gaggtggtgc cgcgtctcga gagccgtgag gtcaccgctg taccggcgac
     2941 cgaatactg
//
LOCUS       DASTNL010000034         7652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1323226, whole genome shotgun sequence.
ACCESSION   DASTNL010000034 DASTNL010000000
VERSION     DASTNL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323226"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFQ96_00950"
     CDS             complement(<1..601)
                     /locus_tag="VFQ96_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU0256387.1"
                     /translation="MPETSQSAKTLRPALSEIEEARSIVSKAAQVTPLEFSRYLSEEL
                     GAPVWLKCENLQRTGSYKIRGAYNRITRLNAEERLRGVVAASAGNHAQGVAFAARELG
                     ISSTIFMPLGVALPKLQATRNYGANVVLGGDTVAEPLRAAAQFAEETGAVFIPPFDHR
                     DIIIGQATLGLEILDQAPQTETILVPVGGGGLLSGIASAA"
     gene            complement(709..1917)
                     /locus_tag="VFQ96_00955"
     CDS             complement(709..1917)
                     /locus_tag="VFQ96_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0256388.1"
                     /translation="MAAPRRSRSPRLRHVARGLARRRTASGDGMLVPRGVRVAGAWSW
                     RLLVIGAAVAVFIFIVVQLRLIVVPVMVAILLTALLTPAVDWLQRHRWPRVVAVAVTF
                     LLALIVVGALVALAVWQVRLGAPDLRHRSVQVFDELRTWLRNGPLGLSNHEIVAFRNR
                     IGAALGSDSQFVINGALSVGSKVGHVAVGFVLAIFSTLFILFDGRAIWGWTVRLFPRR
                     ARAAVDGAGRSGWLTLVSFVRVQILVASIDTVGIGLGVLLLGLPLVVPIAVLVFLGSF
                     VPVVGAVVTGAIAVFIALVYNGWVVALLMLAVVLLVQEVESHVLQPLIMGTAVKVHPL
                     AVVLAVAAGTLLAGIPGALFAVPMAAVANVMIRYVASGTWRGGPIDEADGSVWRVVPV
                     VRRRRGLPRA"
     gene            complement(1959..2855)
                     /locus_tag="VFQ96_00960"
     CDS             complement(1959..2855)
                     /locus_tag="VFQ96_00960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU0256389.1"
                     /translation="MYRAIARNKRNTFVILVVFFAIIAALAWLANAAYGSGGYVIFIV
                     VLVIAAIYALVQYFSAGSQAVALSGARPVTKDEAPRLWRTVENLTIATGLPMPKVYVI
                     DDPAPNAFATGRDPDHAVVAATSGLLGMMDDAQLEAVMAHELGHVRNYDIRVSMIVFG
                     LVVAVGIVADIFLRMSFYGGLRGGRNNNNGGNPVVLVGGLVAMIAAPLVATLVQLAVS
                     RQREYLADATSAMTTRNPEALAGALAKLGEYGRPMARQSSAMSHLWIADPARPGLAAR
                     LLSTHPPIADHIARLQKMGGGF"
     gene            complement(2865..3431)
                     /locus_tag="VFQ96_00965"
     CDS             complement(2865..3431)
                     /locus_tag="VFQ96_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU0256390.1"
                     /translation="MQWLIPVLILAAIVVILGIYFWAAYNSLVTLRVRVDEAWSDITV
                     QLKRRADLIPNLVETVKGYASHERGVFESVTTARAETLSAQGPAEASAAEGHMQSALK
                     SIFAVAEAYPQLQASQNFLRLQADLVDAEDKIQAARRFYNGGVREFNTKIAVFPNNVF
                     ARRLGFTGRDFFEVEDIAAISEPPRVQF"
     gene            complement(3566..3639)
                     /locus_tag="VFQ96_00970"
     tRNA            complement(3566..3639)
                     /locus_tag="VFQ96_00970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3603..3605),aa:Leu,seq:taa)
     gene            complement(3695..5092)
                     /locus_tag="VFQ96_00975"
     CDS             complement(3695..5092)
                     /locus_tag="VFQ96_00975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0256391.1"
                     /translation="MPKILIVGGGYAGFYTAWKLEKWLRAGEAEVTIVDPRPYMTYQP
                     FLPEVAAGSIEPRDAVVSPRRHLKRTKVVAGEVRHVDHQSKTATIAPLVGEAYEFSYD
                     VVVVTAGAVSRTFPIPGVAEQAIGLKNIEEAVAIRDRLVDNFAKAALLPAGPERDRLL
                     TVIVVGGGFAGIEIFGELRSYASALVRRYPEISFEDTHFHLIEAMGRIMPEVSLPTSK
                     WVINNLATRGAEIHLETQLTSAVDGVIQLSTGETMESDLIIWTAGVMANPKVTRNTDL
                     PVDQRGRIKTRADLRVGDDDEIVDGAWAAGDVAAVPDLTGGGVAGMCVPNAQHAVRQG
                     KLLAKNIVAVLRGEKPKNYVHKNLGAVAGIGLGIGVYQKGNLGIKGWIAWLMHRGYHG
                     LALPTWERKIRVIGNWIINLFGGRDIVSLSDRQVPRANFEEFAARPKPAAAKKTPAAE
                     QKAAPKAVKEPAPTK"
     gene            complement(5132..6433)
                     /locus_tag="VFQ96_00980"
     CDS             complement(5132..6433)
                     /locus_tag="VFQ96_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0256392.1"
                     /translation="MRLPVRRPAGRPARSRGVVALRLAGLAAATALTASFVVAGASPA
                     HADHVRDMEYWLSQNTVRQAWRTSEGRGVTVAVIDTGVDGRVADLRGAVTGGADLSGL
                     GSPDGQTPVGEAGESGHGTWVASLLAGRGTGDGSGLIGVAPEASLLSVSVGFGVSSEA
                     ASDAQIAEAVRWSVDHGASVINMSLTRNTLGWPRSWDSAFQYAFDHNVVVVAAAGNRG
                     SGTDEVGAPATIPGVLAVAGVDRQGVASTDASAQGITLGVAAPSEQLVGADPDGSYVQ
                     WDGTSGAAPIVAGVVALVRAAYPRLDAANVINRVIRTATPVGRVPSPLYGYGIVNADK
                     AVTAAVSPVETNPLGSLRDWVRLHRRAAATPEPSSPAGNRAAMPPATLAPEAAGPGPG
                     LGWQGLTGAVLPVAVAAGILLFALALWLAVMRYFRRGTDKG"
     gene            complement(6418..6915)
                     /locus_tag="VFQ96_00985"
     CDS             complement(6418..6915)
                     /locus_tag="VFQ96_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEU0256393.1"
                     /translation="MRPPFRPPDAADIAAVSRQLGRPARGVVGIAARCVCGAPAVVAT
                     APRLAGGAPFPTFYYLCHPAAVAAVSRLEAEHVMAELAGLVAADAGVRDSYRAAHRAY
                     LADRESFAVVPEIAGVSAGGMPDRVKCLHALVAHSLAAGPGVNPVGDRALARCGWSPA
                     VCACR"
     gene            complement(6912..7364)
                     /locus_tag="VFQ96_00990"
     CDS             complement(6912..7364)
                     /locus_tag="VFQ96_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU0256394.1"
                     /translation="MAASRGRDARRPHPLLRDIHASAFWVLMLAILVLGVVILSPTVH
                     LYVTQQNQIAALRSAVGGEQHRLDTTRAQAARWNDPSYVRSEARERLYFVMPGETSFL
                     VIDDLPHATKRGAGAKVQKNVQTAKGDWASTLFASVVSAGVTSGASPR"
     gene            complement(7498..>7652)
                     /gene="eno"
                     /locus_tag="VFQ96_00995"
     CDS             complement(7498..>7652)
                     /gene="eno"
                     /locus_tag="VFQ96_00995"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691008.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0256395.1"
                     /translation="AVATGGGQIKTGAPARSERVAKYNQLLRIEEELGDAARYAGRAA
                     FAHLAG"
BASE COUNT         1235 a         2622 c         2727 g         1068 t
ORIGIN      
        1 gggccgccga ggcgatgccg gaaagcaacc ctccgccgcc gaccgggacg aggatcgttt
       61 cggtctgggg cgcctggtcg aggatctcga ggccgagggt cgcctgcccg atgatgatgt
      121 cgcggtgatc gaacggcggg atgaagaccg cgccggtctc ttcggcgaac tgggcggcgg
      181 cacgcaacgg ctccgcgacc gtatcgccgc cgagcacgac gttcgcccca tagttgcggg
      241 tcgcctgcag cttcggcaag gccacgccca gcggcatgaa gatggtcgag gagatgccga
      301 gctcgcgggc cgcgaatgcg acgccctgcg cgtggttgcc ggccgaggcg gcgacgacgc
      361 cgcggagacg ctcctcggcg ttcaagcggg tgatgcggtt gtaggcgccg cgaatcttgt
      421 aagagccagt gcgctgcagg ttctcgcact tcagccagac gggcgcgccg agctcctccg
      481 aaaggtagcg ggaaaactct aacggcgtga cctgggcggc cttcgagacg atgctccggg
      541 cctcttcgat ctccgacagg gcgggtcgaa gggtcttcgc actctgagag gtttccggca
      601 ttgcaccagt ctatggtcgg ggtgccggga tcccggcggc gcgatccttg accctgccga
      661 aggagcacac cgcgacatcg ccggatctca cggcgtgcag atcgcgcgct aggcgcgcgg
      721 caggccgcgc cggcgccgta ccacgggcac cacgcgccac acgctgccgt cggcctcgtc
      781 gataggcccg ccccgccacg taccggaggc gacatagcgg atcatgacgt tcgcgacggc
      841 cgccatcggc acggcgaaca acgccccggg gatccccgcc agaagggtgc cggccgcgac
      901 ggcgagcacg acggcgaggg ggtgcacctt caccgcggtg cccatgatga ggggctgcag
      961 cacgtggctc tcgacctctt gcacaaggag gacgacggcg agcataagaa gcgccacaac
     1021 ccacccgttg tagacgagcg cgatgaagac ggcgatcgca cccgtcacga ccgcgccgac
     1081 gaccggcaca aaagagccaa ggaagacgag gacggcgatc ggcacgacga ggggcaggcc
     1141 gagcaacaac acgcccaaac cgatgccgac cgtgtcgatg gaggcgacaa ggatctgcac
     1201 gcggacgaag ctgacgagcg tgagccagcc ggaacggccg gcaccgtcga ccgccgcgcg
     1261 agcccggcgc ggaaagagcc gcaccgtcca gccccagatc gcccggccgt cgaaaaggat
     1321 gaagagggtt gagaagatcg ccaagacgaa cccgaccgcg acatggccga ccttcgagcc
     1381 gacggagagc gctccgttga tgacgaactg gctgtcgctg ccgagcgccg cgccgatccg
     1441 gttgcggaac gccacgatct cgtggttgct gagcccgagc gggccattcc gcaaccaggt
     1501 gcgcagttcg tcgaagacct gaacgctgcg atgccgaagg tcgggggcgc cgagccgcac
     1561 ctgccacacg gccagggcga ccagcgcgcc gacgacgatg agggcgagga ggaacgtgac
     1621 ggcgaccgcc acgacgcgag gccaccggtg ccgctgcagc cagtcgacgg ccggtgtcag
     1681 aagcgcggtc agaaggatgg cgaccatcac cggcacgacg atgagccgca gctggacgac
     1741 gatgaagatg aagaccgcga cggcggcgcc gatgacgagg agccgccacg accaggcccc
     1801 ggcgacccgc acgccgcgcg gcacgagcat gccgtcaccc gaagcggtcc ggcgcctcgc
     1861 cagccccctg gcgacgtggc gcagacgcgg ggatctgctg cgtcgggggg ccgccatggg
     1921 cctcagtcta tgggcgctca cccgacgctc cggcggcgtc agaaaccgcc gcccatcttc
     1981 tgcagccggg cgatgtggtc ggcgatcggc ggatgggtgc tgagcagtct ggcggcgagc
     2041 cccggtcggg ccgggtcggc gatccacaga tgtgacatcg cggaactctg gcgcgccatc
     2101 ggccggccgt attcgccgag tttcgcaagc gcgccggcca gcgcctcggg gttgcgtgtc
     2161 gtcatcgcac tcgtcgcgtc ggcgaggtat tcccgctgcc gagaaacggc gagctgcacg
     2221 agcgtggcga ccagcggagc cgcgatcatc gcgacgaggc cgcctacgag aacgaccggg
     2281 ttaccgccgt tgttgttgtt ccgaccgccg cgcaggcccc cgtagaacga catgcgcaag
     2341 aagatgtcgg cgacgatgcc gaccgcgacg acaaggccga agacgatcat ggagacgcga
     2401 atgtcgtagt tgcgcacgtg gccgagctcg tgcgccatga cggcctcgag ctgtgcgtca
     2461 tccatcatgc cgaggagccc gctcgtggcg gccaccaccg cgtggtcagg gtcgcggccg
     2521 gtcgcaaacg cgttcggcgc cgggtcgtcg atgacataca ccttcggcat cggcaagccc
     2581 gtggcgatgg tgagattctc gacggtgcgc cagagcctcg gcgcctcgtc cttcgtcacc
     2641 ggccgggcgc cggaaagcgc gaccgcctgg gagccggcgg agaaatactg gacgagcgcg
     2701 tagatcgcag cgatcaccag cacgacgatg aagatgacgt agccgccgct gccgtaggcg
     2761 gcgttggcga gccaggccag agcggcgatg atggcgaaga acaccacgag gatgacgaag
     2821 gtgttccgct tgttgcgcgc tatcgctcga tacatggggc gtcgctagaa ctgcacgcgc
     2881 ggcggttcgg agatcgcggc gatgtcctcc acctcgaaga agtcacggcc ggtgaagccg
     2941 agccggcggg cgaagacgtt gttcgggaac accgcgatct tcgtgttgaa ttcccgcacc
     3001 ccgccgttgt agaagcggcg cgccgcctgg atcttgtctt cggcgtcgac caggtcggcc
     3061 tgcaggcgca ggaagttctg gctggcctgc agctgcgggt aggcctcggc gacggcgaag
     3121 atcgacttca gggcggactg catgtgcccc tcggccgcag acgcctctgc cgggccctgg
     3181 gcggagagcg tctcggcgcg cgccgtcgtc accgattcga acacgccgcg ttcgtggctc
     3241 gcatagcctt tgaccgtctc gacgaggttc gggatgaggt cggcccgccg tttcagctgc
     3301 accgtgatgt cgctccacgc ctcgtcgaca cgcactcgca gggtgacgag cgagttgtag
     3361 gcggcccaga agtagatgcc gaggatcacc acgatcgcgg cgaggatgag gaccggaatc
     3421 aaccactgca tggaaactcc tcggtgcggt agtgggcggg gccgagccag gttcgacagg
     3481 cacgccgtcg cggtggagct gactggccag catcctaccg ggcgtggctt gagaggccct
     3541 ggggaggcgc acaggcccgc cgtcgtaccc ccagccggac tcgaaccggc actgttacga
     3601 ttttaagtcg tctgcctctg ccaattgggc tatgggggcc cgccgggtgc cggccgcaca
     3661 cggcggcacg gcgcccggct gcatcatctc gaccttattt ggtcggcgca ggctccttga
     3721 cagctttcgg ggcggccttc tgctcggccg ccggcgtctt cttcgccgcg gcgggcttcg
     3781 gacgggcggc gaactcctcg aagttggcgc ggggcacctg ccggtcgctc agcgagacga
     3841 tgtcacggcc gccgaacagg ttgatgatcc agttgccgat gacgcggatc ttgcgctccc
     3901 aggtgggcag cgccaggccg tggtagccgc ggtgcatgag ccaggcgatc cagcccttga
     3961 tgccgaggtt gcccttctgg tacacgccga tgccgagccc gatgccagcc accgcgccga
     4021 ggttcttgtg gacgtagttc ttcggcttct cgccgcgcag caccgcgacg atgttcttgg
     4081 cgagcagctt gccctggcgc acggcatgct gcgcgttcgg cacgcacatg ccggcgacgc
     4141 cgccgcccgt caggtcgggc accgccgcga catcgcccgc cgcccacgcg ccgtcgacga
     4201 tctcgtcgtc gtcgccgacc cgcagatcgg cgcgggtctt gatgcggccg cgctggtcga
     4261 ccggcagatc ggtgttgcgg gtgaccttcg ggttcgccat gacgcccgcg gtccagatga
     4321 tgaggtcgga ttccatcgtc tcgccggtgg agagctggat cacgccgtcg accgcgctcg
     4381 tcagctgggt ctcaaggtgg atctcggcgc cgcgggtggc gaggttgttg atcacccact
     4441 tgctcgtcgg caacgacacc tcgggcatga tgcgccccat cgcctcgatc aggtggaagt
     4501 gggtgtcttc gaaactgatc tctgggtagc gccgcacgag ggcggaggca taggagcgca
     4561 gctcgccgaa gatctcgatg cccgcgaagc cgccgcccac gacgatcacg gtgagcaacc
     4621 ggtcgcgctc cgggccggcg ggcaggagcg ccgccttcgc gaagttgtcg accaggcggt
     4681 cgcggatcgc gacggcctcc tcgatgttct tcagaccgat cgcctgctcg gcgacgcccg
     4741 ggatggggaa cgtgcgtgag acggcgccgg cagtcaccac gacgacgtcg taggagaact
     4801 cgtacgcctc gccgacgagc ggggcgatcg tcgcggtctt cgactggtgg tcgacgtgcc
     4861 gaacctcgcc cgcgacgacc ttcgtgcgct tcaggtgccg acgaggagag acgaccgcgt
     4921 cgcgcggctc gatcgacccg gcggcgacct cggggagaaa aggctgatag gtcatgtacg
     4981 ggcgcgggtc gacgatggtg acttcggcct cccctgcacg cagccacttc tctagcttcc
     5041 acgcggtgta gaagcccgcg taaccgccgc cgacaatcag gattttgggc acagaaaagt
     5101 ctcctcggac tcgcgtaagt attctcctaa tttacccctt atcggtgccg cgtctgaaat
     5161 aacgcatcac ggccagccac agcgccagcg cgaacagcag gattccggcg gcgacggcga
     5221 ccggcaagac agccccggtg aggccttgcc agccgagccc cgggccgggc ccggcggcct
     5281 cgggagccag cgtcgcggga ggcatggcgg cccgattccc ggcgggcgac gagggctcgg
     5341 gcgtcgccgc ggcgcgccga tgcagcctga cccagtcgcg gaggctgccg agcgggttcg
     5401 tctcgaccgg cgacaccgcc gcggtgaccg ccttgtcggc gttcacgatc ccgtagccgt
     5461 agagggggct cggaacccgc ccgaccgggg tcgcggtgcg gatgacccgg ttgatgacgt
     5521 tggcggcgtc gagcctcggg taggcggcac gcaccaacgc gacgacgccg gcgacgatcg
     5581 gcgcggcgcc gctcgtgccg tcccattgca catagctccc gtcggggtcg gcacccacca
     5641 gctgctcgct cggcgcggca acgccgaggg ttatgccctg cgccgaagcg tcggtgctcg
     5701 cgaccccttg gcgatcgacg cccgcgacgg ccagaacccc cggtatcgtc gccggcgcgc
     5761 ccacctcgtc ggttccgctg ccccgattgc ctgccgccgc gaccacgacg acgttgtggt
     5821 cgaacgcgta ctggaacgcg gagtcccacg agcgcggcca gccgagcgtg ttgcgggtga
     5881 gcgacatgtt gatgacggag gcgccgtggt cgaccgacca gcgcaccgct tcggcgatct
     5941 gcgcgtcgct ggccgcctcg gaggagacgc cgaacccgac ggaaacgctc aagaggctcg
     6001 cctcgggagc caccccgatg aggccgctcc cgtcgcccgt gccccggccg gcgagaagag
     6061 aggccaccca ggtgccgtgc cccgactcgc cggcctcgcc gacgggtgtc tgcccgtctg
     6121 gggagccgag gcccgagagg tcggcgccgc cggtgaccgc accgcgcagg tcggcgaccc
     6181 gcccatccac cccggtgtcg atgacggcga ccgtgacccc gcgaccttca ctcgtgcgcc
     6241 acgcctgccg aaccgtgttc tggctgagcc aatactccat gtcgcgcaca tggtcggcgt
     6301 gcgcgggaga cgcgcccgcc acgacgaacg aggccgtcag tgccgtcgcg gcggcgaggc
     6361 cggcgaggcg gagggcgacg acgccgcgcg agcgggccgg gcgcccggcc ggccgcctca
     6421 ccggcaggcg cataccgcgg ggctccagcc gcagcgggcg agcgcccgat cgcccacggg
     6481 attcacaccg ggcccggcgg caaggctgtg cgccacgagg gcgtgcagac atttgacccg
     6541 atcgggcatg ccgccggccg agacgccggc gatctcggga acgacggcga acgactcccg
     6601 atcggcgaga taggcgcggt gggccgcacg gtacgagtct cggacgccgg cgtctgcggc
     6661 cacgaggcct gcgagctccg ccatgacgtg ttccgcctcg agacgcgaga cggcggccac
     6721 cgcggccggg tggcacaggt agtagaacgt cggaaagggc gcccccccgg ccaggcgcgg
     6781 cgcggtcgcg acgacggcgg gcgcgccgca gacgcacctt gcggctatgc cgacgacccc
     6841 gcgggcgggt ctgcccaatt gccgtgagac cgcggcgatg tctgcagcgt ccggcgggcg
     6901 aaacggcggc ctcatcgcgg cgacgcaccc gacgtcacgc cggcggagac gacggaggcg
     6961 aagagcgtgg aggcccagtc ccctttcgcc gtctgcacgt ttttctgcac cttcgcaccg
     7021 gcaccccgct tcgtcgcatg cggaaggtcg tcgatcacga gaaagctggt ctcgccgggc
     7081 atcacgaaat agagacgttc ccgagcctcg gaacgcacat aactggggtc gttccagcgc
     7141 gcggcctgcg cccgggtcgt gtcgagccgg tgctgctcgc cgcccaccgc cgaacgcagc
     7201 gcggcgatct ggttctgctg ggtgacgtaa aggtgcacgg tcggcgagag gatgacgacg
     7261 ccgagcacga ggatggcgag catcaagacc cagaaggcag aggcgtgaat gtcgcgcaac
     7321 agcgggtgcg gccgtcgggc atctctcccc cgtgacgcgg ccatcgtcac ccccacctct
     7381 cgcccgagcc gactgtccgc ccgcgaccct cgttctcgcg ccgattctac gagtcggcgc
     7441 cacctcgtgc gggcggcgca ccggccggcc gcacgagagg cgcgtgcgtt ggggtgctca
     7501 gccggccagg tgggcgaacg cggcccggcc cgcgtaccgc gcggcgtcgc ccagctcttc
     7561 ttcgatgcgc agaagctggt tgtacttcgc gacccgctcg ctgcgggcgg gggcgccggt
     7621 cttgatctgc ccgccgcccg tggcgacggc ga
//
LOCUS       DASTNL010000035         4434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_767878, whole genome shotgun sequence.
ACCESSION   DASTNL010000035 DASTNL010000000
VERSION     DASTNL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767878"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /gene="sepH"
                     /locus_tag="VFQ96_01000"
     CDS             <1..1095
                     /gene="sepH"
                     /locus_tag="VFQ96_01000"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEU0256396.1"
                     /translation="GKLIATDDSGSQYLIRLRSVPAPGARPPARNAVPAAKTTPRQIQ
                     AYIRGGMSAADVAELTGTELADVERFEGPILAEREYVVSSALGVAVHVADGVGGDHRT
                     TFGAAIRERLEALSATGERWASWREPEGGWIVKLAFTVDEIDHDARWAFDPRRRTLES
                     LNPEATTLSQQDVSPGRSLIPRLRVVPTGDPDTSRFDSAAFTFDDRSRERPATPAHPA
                     QRTAPSPAGAPASPFAIEAVRTTPLPPAPNHQTADLLAALRNKRLESARSLEGEGEPT
                     AGLTQEPDDGLPEPPALLGDERVFGGHAVGGRAEGPGADDPRLADGNEGSGKHDKPVR
                     QRPTGARRGRASIPSWDEIVFGARTDDDPA"
     gene            complement(1192..2337)
                     /locus_tag="VFQ96_01005"
     CDS             complement(1192..2337)
                     /locus_tag="VFQ96_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEU0256397.1"
                     /translation="MDAMLPVLSPSGPSLAEVMPAGLAALDGDENPLGLPALRSLIVV
                     LVDGLGASLVARHSGHLRFLASRIHGSTIDAVFPSTTAAGISSLCTGLRPGRHGMVGY
                     RVLDPDNDRVVNQLRDWDALMTPEAWQTQRTVFEKAAARGIPVAAVGQSRYRRSGFTR
                     AVLRGARYLGAERLEDRFDAAARFVAEHDRSLVYLYVHELDVAGHAHGCDSLAWCTAA
                     EDVDAALAGLERRGGAGDAVLVTADHGMLDVPPARHVLFDQGPSALLAGVRHVAGEPR
                     CLQLHLEHGSGPAAAERVAEAWREVEGEAAWVATRGQAAEAGWFGPEVTDQAAARLGD
                     VFVAARDRIAFYDSRTASARSRAMVGQHGSLTDEEMTVPLVRLGAAA"
     gene            2436..>4434
                     /locus_tag="VFQ96_01010"
     CDS             2436..>4434
                     /locus_tag="VFQ96_01010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU0256398.1"
                     /translation="MTPRHDTAPTPERIEDVDVVSEMQDSYLEYAYSVIYSRALPDAR
                     DGLKPVQRRILFQMSQMGLRPDRGHVKSARVTGEVMGKLHPHGDSAIYDALVRMAQDF
                     TLRVPLIDGHGNFGSLDDGPAAARYTEVRLAPPALAMTENLDEDVVTFVPNYDDQLTQ
                     PSVLPSAFPNLLVNGASGIAVGMATNMPPHNLGEVIDAARHLIAHPEASVEELMRFVP
                     GPDLPGGGIIVGREGIAEAYTSGRGIFKTRAKASIERVTARRTGIVITELPYLVGPEK
                     VIEKIKQGVRSKKLQGISDVDDLSDGTNGLRLVVGVKAGFDPQAVLARLYQHTPLEDS
                     FAINNVALVDGSPRTLGLKELLEVYIDHRIQVVTRRSDHRLQKHLDRLHLVEGLLIAV
                     LDIDEVIQLIRDSDDADEARGRLMKVFDLSRVQSEYILELRLRRLTKFSRIELESERD
                     ELTASIERLRALLADDGLIRRQVSEELAAAAEEFATARRTVLLDERAEDASSGPTTAL
                     EVPDEACRVVLTASGKILRTDAALPADGGAGPGEGLISPASPARGRRARRIRHDAVAS
                     VVVTTTRARLGALTSAGRVLSCTPVELPAMPGAGLQFTAGVEASAYAAASAGERVVAL
                     VVAGEVPFAIGTAAGVVKRVTLREWPAKDAFPVISLKPGDRV"
BASE COUNT          709 a         1622 c         1536 g          567 t
ORIGIN      
        1 ggcaagctca tcgcgacgga cgattcgggc tcgcaatacc ttatacggct gcgttccgtt
       61 ccggccccgg gagcccgccc cccggccagg aacgccgtgc cggccgccaa aacgacgccg
      121 cgacagattc aggcctacat acgcggcggc atgtctgcgg cggatgtcgc cgaactgacc
      181 ggcacggaac tggccgacgt ggaacgtttc gaagggccca tcctcgcgga gcgcgagtac
      241 gtggtcagca gcgcactcgg cgtagccgtc cacgtggccg acggggtcgg aggcgatcac
      301 cgcacgacgt tcggcgcggc catccgcgaa cgcctcgaag ccttgtcggc cacgggcgaa
      361 cgctgggcga gctggcggga gcccgagggc ggttggatcg tgaagctggc gttcaccgtc
      421 gacgagatcg accacgacgc caggtgggcc ttcgacccgc gccggcgcac cctcgaatcg
      481 ctcaaccccg aggcgaccac cctctcccag caagacgtct cgccgggccg cagcctcata
      541 ccgcgcctgc gcgtcgtgcc caccggcgac ccagatacct cacgcttcga cagcgcggcg
      601 ttcaccttcg acgaccggtc acgggagcgg cccgcgacgc cggcgcaccc cgcgcagcgc
      661 accgctccct cgcccgccgg agcgccggcc agcccgttcg ccatcgaggc ggtgcgcacg
      721 acccccctcc cgccggcgcc gaaccaccag accgcagacc tcttggcggc gttgcggaac
      781 aaacgcctcg agagcgcgag aagtctcgaa ggcgagggcg agcccaccgc cgggctcacc
      841 caggagccgg acgacggcct ccccgagccg cctgccctcc ttggcgacga gcgcgtattc
      901 ggcggccatg ccgtgggtgg gcgagcggaa ggcccgggcg ccgacgaccc gcgtctcgca
      961 gacggcaatg aggggagcgg caagcacgac aaaccggtgc ggcagcgccc gaccggcgcg
     1021 cgccgcgggc gagcatcgat ccccagctgg gacgagatcg tcttcggcgc ccgcaccgac
     1081 gacgatcccg cctgaccgcg cccgtcggag gaaggggcga cccgccccgc gcgaccccgc
     1141 ccaaggccgg gtcggccgtg gcagccgggg cggccgggtg ccgtaaggaa ctcaggcggc
     1201 cgcgccgagc cgcaccaacg gcacggtcat ctcctcgtcg gtcaaagagc cgtgctggcc
     1261 caccatggca cggctgcgcg cgctcgccgt gcgggagtcg tagaaagcga tgcggtcgcg
     1321 ggcggcgacg aatacgtcgc cgagccgggc ggcggcctgg tcggtgacct ccgggccgaa
     1381 ccagccggcc tcggccgcct gcccacgggt ggcgacccat gccgcctcgc cctcgacctc
     1441 acgccacgcc tcggccacac gctcggccgc cgcgggcccc gagccgtgct ccaggtgcag
     1501 ttgcaggcag cgcggctcgc cggcgacgtg ccgcacaccg gcgagaaggg ccgacggccc
     1561 ttggtcgaag agcacgtggc gggcaggcgg cacgtcgagc atgccgtgat cggccgtcac
     1621 caagaccgcg tcccccgcgc cgcctcggcg ttccaggccg gcgagcgccg catcgacgtc
     1681 ttccgccgcg gtgcaccagg cgagcgagtc gcaaccgtgg gcgtgccccg cgacgtcgag
     1741 ctcgtgcacg tacaggtaga cgaggctgcg atcgtgctcg gcgacgaatc gagccgcggc
     1801 gtcgaaacga tcctccagac gttcggctcc caggtaacgg gcgccgcgca ggacggccct
     1861 cgtgaagccg gagcgccggt agcggctctg cccgaccgcc gccacgggga tgccccgcgc
     1921 ggcggccttc tcgaacaccg tccgctgggt ctgccacgcc tccggcgtca tgagcgcgtc
     1981 ccagtctctc agctggttga cgacccggtc gttgtcgggg tcgagcaccc ggtagccgac
     2041 catgccgtgc cggccggggc gcagcccggt gcacaggctg ctgatgccgg ccgccgtcgt
     2101 cgacgggaac accgcgtcga tcgtggaacc gtgaattcgc gacgcgagaa agcgcagatg
     2161 cccggaatgt cgggcgacga gcgaggcgcc gaggccgtcg acgagcacca cgatgagaga
     2221 gcgcagagcc ggcagcccca gaggattctc gtcgccgtcg agtgcggcca ggcccgccgg
     2281 catgacctcc gccaggctgg gccccgacgg cgagagcacc ggtagcatgg catccatcag
     2341 gctcagtctg ccatagggcg taccggcacc cgcacctcgc cgggccggcg cacggcgggc
     2401 agaggcgccg ccatccgccg cagagattgc ttttcatgac cccacgccac gacacggcac
     2461 cgacccccga acgcatcgaa gacgtcgatg tcgtctcaga gatgcaagac tcctacctcg
     2521 aatacgcgta ctcggtgatc tactcccgag ccctgcccga cgcccgagac gggctgaagc
     2581 cggtgcagcg acgcatcctc ttccagatga gccagatggg tcttcgcccc gaccgcggcc
     2641 acgtcaagtc ggcacgcgtg accggcgagg tgatgggcaa gcttcacccg cacggcgaca
     2701 gcgcgatcta tgacgccctg gtgcgcatgg ctcaagactt cacgctgcgg gtcccactga
     2761 tcgacgggca cggcaacttc ggctctctgg acgacggccc cgccgcggcg cgctacacgg
     2821 aggttcgcct cgctcctccc gcgttggcga tgacggagaa cctcgacgaa gacgtcgtca
     2881 cgttcgtgcc gaactacgac gaccagctca cccagccgag cgtgctaccg agcgcctttc
     2941 ccaacctgct cgtgaacggc gccagcggga tagcggtcgg aatggcgacg aacatgccac
     3001 cgcacaacct cggcgaggtg atcgacgcgg cccgccacct catcgcccac ccggaggcga
     3061 gcgtcgagga gctgatgcgt ttcgtccccg gccccgacct gccgggcggc gggatcatcg
     3121 tcgggcgaga aggcatcgcc gaggcataca cgtcgggccg cggcatcttc aagacgcgcg
     3181 cgaaggcgtc gatcgagagg gtcacggcgc gccgcacggg catcgtcatc accgagctgc
     3241 cgtacctcgt gggccccgag aaggtgatcg agaagatcaa gcagggggtg cgttcgaaga
     3301 agctgcaagg catctcagac gtcgacgacc tgagcgacgg aaccaacggc ctgcggctcg
     3361 tcgtcggggt gaaggccggg ttcgacccgc aggcggtctt ggcgcgcctc taccagcaca
     3421 ccccgctgga ggacagtttc gccatcaaca acgtggcgct cgtcgacggg agtccccgca
     3481 ccctcgggct gaaggagctg ctcgaggttt acatcgacca tcggatccag gtcgtcacgc
     3541 gtcggagcga ccaccggctg cagaagcacc tcgaccggct gcatctcgtc gagggccttc
     3601 tcatcgccgt gctcgacatc gacgaggtca tccaactgat tcgtgacagc gacgatgccg
     3661 acgaggcccg cgggcgcctc atgaaggtct tcgacctcag ccgggtgcag agcgagtaca
     3721 tcctcgaatt gaggctgcga cgcctgacga agttctcccg gatcgagttg gagtcagaac
     3781 gcgacgagct gacggccagc atcgagcggc tacgggccct cttggccgac gacgggctga
     3841 tccgtcggca ggtctcggaa gagctcgccg cggccgccga ggagttcgcg accgcccgcc
     3901 gcaccgtgct cctcgacgaa cgggccgagg atgcctcgtc gggcccgacg accgcgctgg
     3961 aggtgcctga cgaggcatgc cgggtcgtct tgacggcgag cggcaagatc ctgcgcaccg
     4021 atgcggcgct gcccgccgac ggcggggcgg gccccggcga ggggctcatc agcccggcct
     4081 cgccggcacg cggccgccgg gcccgtcgga tccggcacga cgcggtcgcg tccgtggtcg
     4141 tgacgacgac ccgcgcacgg ctgggagcgc tgacctccgc cgggcgggtc cttagctgca
     4201 cgcccgttga gcttccggcg atgcccggtg caggtctcca gttcacggcc ggcgtcgagg
     4261 cctcggccta cgccgcggcc tcggccggcg agcgggtcgt cgcgctcgtc gtcgcgggcg
     4321 aggtgccgtt cgccatcggt acggcggccg gcgtcgtcaa gcgggtcacg ctgcgggaat
     4381 ggccggccaa ggacgcgttc ccggtcatct cgctgaagcc cggcgatcgc gtcg
//
LOCUS       DASTNL010000036         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_213220, whole genome shotgun sequence.
ACCESSION   DASTNL010000036 DASTNL010000000
VERSION     DASTNL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213220"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1718)
                     /locus_tag="VFQ96_01015"
     CDS             complement(237..1718)
                     /locus_tag="VFQ96_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cytidylyltransferase/SDR family
                     oxidoreductase"
                     /protein_id="HEU0256399.1"
                     /translation="MGSDAASEAGAEGRPARQVDKAIAVILAGGVGVRAGLGMPKQLA
                     RIAGKSILEHTVAAICSSPVIDEVLIMMEAAHLGEAAALKDSGRYPKLVGVFSGGASR
                     NDTTRLAIDQVAARSGAGNAKVLFHDAVRPFVSHRILRDCVAALDRFNAVDTAIPSAD
                     TIIQVGDTRAIITDVPRRDRLRRGQTPQAFRLETIRRAYRAAEADPGFAATDDCTVVL
                     RYLPEVPIFVVDGSEENMKITQAIDVVLADKLFQLKTHDPDVFDREARGRMAGKVLVV
                     FGGSEGIGKALVETAEAWGAKVFSFSRGTTGTHVEAREEVRAALSLAHDEAGRVDYVV
                     NCAGILTIGELETTTDDDLRASLEVNLVAPIIIAQEAVGYLRQSAGQLLFFTSSSYTR
                     GRARYGVYSAAKAAIVNLTQSLAEEWHDDDVRVNCVNPERTHTPMRTHAFGEEPAGTL
                     LSPEIVALACLDTLLSPATGQVVDVRRHAAAEASSAAKEPAQA"
     gene            complement(1818..>2305)
                     /locus_tag="VFQ96_01020"
     CDS             complement(1818..>2305)
                     /locus_tag="VFQ96_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide production protein"
                     /protein_id="HEU0256400.1"
                     /translation="LVAAYVFRAPLLQALDARSIFSVRYNLWLQIWHLNGDNPLVGWG
                     WSGLWWRHIPPYDVLNFLLGTAHDSALDAYLDVYLQVGLAGLVLFGLMVALAFGRAWV
                     VASNKRTVVYTWAPLVLVALIVTSVLDSTMLVDWGWLLLVVCCVKASQSLSWRHRLPV
                     R"
BASE COUNT          376 a          779 c          807 g          343 t
ORIGIN      
        1 gtcgccgacg tgatagaggt tggcacggcc tacatcgccg aaaacgacac atctcatgcc
       61 tgtgccgaag cctctcgctg cacagccaag gacaagtggc gcggccggtg aggaaaggca
      121 aggtcggtgc acctgggagc gactgacggc gtcgggcttc cttctgtcac ggcgtgagtc
      181 taacaaccag gtcctgagca acttctctca ttgccgggct ggtgcgtggc gcacgctcac
      241 gcctgcgcgg gctccttggc cgctgacgac gcctcggccg ccgcgtgccg gcgcacgtcg
      301 accacctggc cggtagcggg cgaaagaagc gtgtcgaggc aggcgagcgc gactatctcg
      361 ggcgaaagaa gcgtgccggc cggctcctcg ccgaaggcgt gggtgcgcat cggcgtgtgg
      421 gtgcgctccg ggttgacgca gttcacccgc acgtcgtcgt cgtgccattc ttcggccagt
      481 gactgggtga ggttcacgat ggccgccttc gcggcggaat agacgccgta gcgcgcgcgg
      541 ccgcgcgtgt acgaactcga ggtgaagaac agcagctggc cggcgctctg gcgcaggtag
      601 cccaccgcct cttgcgcgat gatgatcggc gcgacgaggt tcacctcgag gctcgcccgc
      661 aggtcgtcgt ccgtcgtcgt ctcgagctcg ccgatcgtca agatgcctgc acagttgacg
      721 acgtagtcca cccgaccggc ctcgtcgtgt gcgaggctca gggcggcgcg cacctcttcc
      781 ctcgcctcca cgtgggttcc cgtcgtgccg cggctgaagg agaagacctt cgccccccac
      841 gcctcggccg tctcgacgag ggccttgccg atgccctcgc tgccgccgaa gacgaccagc
      901 acctttccgg ccatgcgccc gcgtgcctcc cggtcgaaca cgtcggggtc gtgggtcttc
      961 agctggaaga gcttgtcggc cagcaccacg tcgatggcct gggtgatctt catgttctct
     1021 tccgagccgt cgacgacgaa gatcggcacc tcgggcaggt agcgcaagac gaccgtgcag
     1081 tcgtcggtcg cggcgaagcc ggggtcggcc tcggcggccc ggtaggcgcg gcggatcgtc
     1141 tcgagccgga acgcttgcgg ggtctggccg cggcgcaggc ggtcgcggcg cggcacgtcg
     1201 gtgatgatcg cgcgggtgtc gcccacctgg atgatcgtgt cggccgacgg gatcgccgtg
     1261 tcgaccgcgt tgaaacggtc gagcgccgcc acgcagtctc tcaagatgcg atgactgacg
     1321 aacggccgca cggcgtcgtg gaagagcacc ttggcgttcc ccgcgccgga ccgcgccgcc
     1381 acctggtcga tggccaggcg tgtcgtgtcg ttgcgggagg cgccgccgga gaagacgccg
     1441 accagcttgg ggtatcggcc cgagtctttg agggccgcgg cctcgcccag gtgagccgcc
     1501 tccatcatga tgaggacctc gtcgatcacc ggcgacgagc agatcgccgc cacggtgtgc
     1561 tccagaatgc tcttgccggc gatgcgagcc aactgtttcg gcatgcccag cccggcacgc
     1621 acccccacgc cgcccgccaa gatgacagcg atcgccttgt ccacctgccg cgcggggcgg
     1681 ccttccgcgc cggcctcaga cgcggcgtcg ctccccatgg tcacggcgcg agtctaacaa
     1741 ccgtgccgca gagggcggcg tgcgagagag gctgcgcggg gcggcctcgg ccgaagacgc
     1801 acggcaggcc gcgagcgtca acgcaccggc agccggtgcc gccagctgag ggattgcgac
     1861 gccttgacgc aacagacgac gagcaagagc catccccagt cgacgagcat cgtgctgtcg
     1921 agcaccgacg tgacgatgag cgccaccaag acgaggggcg cccaggtgta gacgacggtg
     1981 cgcttgttcg aggcgacgac ccaggcgcgg ccgaacgcca gggccaccat gaggccgaac
     2041 agcacgagac ctgccaggcc gacctgcagg tacacgtcga ggtaggcgtc taaggcgctg
     2101 tcgtgcgcgg tgccgagcaa gaagttcagc acgtcgtacg gcgggatgtg ccgccaccac
     2161 aggccgctcc acccccaccc gacgagcggg ttgtcgccgt tcaggtgcca gatctgcagc
     2221 cacaggttgt agcgcaccga gaagatcgac cgggcgtcca acgcctgaag caggggagcg
     2281 cgaaagacgt aggcagcgac caggg
//
LOCUS       DASTNL010000037         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_213624, whole genome shotgun sequence.
ACCESSION   DASTNL010000037 DASTNL010000000
VERSION     DASTNL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213624"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..2336
                     /locus_tag="VFQ96_01025"
     CDS             204..2336
                     /locus_tag="VFQ96_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU0256401.1"
                     /translation="MASTVTRHEPPTRRNPVGGLVADDAMPGLLPDGFDDLPLDAPVA
                     PFRLPHLNPVPVVAALCVPLAIAVTLVGMTFSGAFSTGTGLYNPGQLVTYGLPIVRAA
                     HDLVAALTVGLLVLATIVLPGQRKHPGEVSFSQWKAVRWAAYSAAAWFVLAVAGLLLE
                     SVEISGVPFGDPLYPSSLRTFVTTVALGQSLAVSAACILAAGILALFARGQTTVGVAC
                     VLGLAALLPLALSGHAAGSIEHANAVNSLAIHLVGATVWMGGLAGLILLRRHLKTLFG
                     LAVERYSTVALWAFGAVAFSGTVNAALRLGSVAALLTPYGLLVVAKICLLVILGLFGV
                     LHRRLIIPRLRADPTSRALFVRFAAAEVVFLTIAFGVAVALSKSPTPASATPTGADAA
                     REALLGFPLPPPVTALRMFTVWHIDWMWLGLACTLAGGYLWAVAKLRRRGDRWPLHRT
                     LCWLLGCLSLVWLTSGGPDVYGFLHFSSHMIAHIGLMMIAPPLLTLAGPVLLALRALP
                     SRHDGSRGPREWLLWLLHSPYLAFLSKPVVAGLIFAGSLYAFYFSPLFPWAMQDHTGH
                     LFMTLHFLASGYLFFWVFMGVDPGPRTTNYPVLLLTLLGTVAFHGFFGVALMMSNGII
                     APDWWHAVGLQNAAAILADQHTAGGIAWSAGELPMMLAAIGLLYAWMRSDERAAARLD
                     RKADRDGDADLAAYNAQLERIAQHDHTR"
BASE COUNT          281 a          892 c          771 g          436 t
ORIGIN      
        1 gcctggtcgc cgcgaaaggc gtcgtagccg aagacggtgt gcagcacgtc ggcggcgtcg
       61 gagaaccggc gggtctcgcg ccgacccgtc gttaccgtcc cgtctgccac ccggccatgc
      121 tagttgccgt gcccgatctg ggcttattcc gtccaccttg ctggcatcgc ggcttagaat
      181 ggctgcggta tagcacgaga aagttggcat ccaccgtgac gaggcacgag ccccccacca
      241 ggcggaaccc ggtcggcggc ctggtcgccg acgacgcgat gccgggcctt ctccccgacg
      301 gcttcgacga tctgccgctc gacgcgccgg tcgcgccgtt ccgcctgccg cacctgaacc
      361 cggtgcccgt cgtcgcggcg ctctgcgtgc cgcttgcgat cgccgtgacc ctcgtcggca
      421 tgaccttctc gggtgccttc tcgaccggca ccggcctcta caaccctggg cagctcgtca
      481 cctacggtct accgatcgtg cgcgccgcac acgacctggt cgcggccctc accgtgggtc
      541 tgctcgtgct cgcgacgatc gtcttgcccg gccagcgcaa acacccgggc gaggtgagct
      601 tcagccaatg gaaggcggtg cggtgggctg cctatagcgc cgccgcctgg ttcgtgctcg
      661 ccgtcgccgg gctgctcctg gaaagtgtcg agatctcggg ggtgccgttc ggcgacccgt
      721 tatacccgtc gtcgttgcgc accttcgtca ccacggtggc cctcggccag tcgctggccg
      781 tctcggccgc ctgcattctc gcggccggga ttctcgcgct tttcgcgcgc ggccagacca
      841 cggtcggcgt cgcctgcgtc ttaggcctcg ccgccctttt gccgctcgcg ctctccggcc
      901 acgccgcggg ctccatcgaa cacgccaacg ccgtgaactc cctcgccatc cacctggtcg
      961 gggcgaccgt gtggatgggc gggctcgccg ggctgatcct cttgcgccgg cacctgaaga
     1021 ccctgttcgg gctcgccgtc gaacgatact cgaccgtcgc gctctgggcg ttcggcgcag
     1081 tcgccttctc gggcacggtg aacgcggcct tgcggctcgg cagcgtcgcg gctctcctga
     1141 ccccgtacgg gcttctcgtc gtcgcgaaga tctgcctgct cgtgatactc ggcctcttcg
     1201 gcgtgctgca ccgcaggctc atcatccccc gcctgcgagc ggatccgacg agccgggcgc
     1261 tgttcgtgcg cttcgccgcc gccgaggtcg tctttctgac gatcgcgttc ggggtggcgg
     1321 tcgccctgtc gaagagcccg acccccgcct ccgccacccc gacgggggcc gacgcggccc
     1381 gcgaggcgct cctcggcttt cccctgccgc ccccggtgac ggcgctgcgc atgttcacgg
     1441 tatggcacat cgactggatg tggctgggtc tcgcctgtac tcttgccggc ggctacctgt
     1501 gggccgtggc gaagctgcgt cgccgcggag accgctggcc gctgcatcgc accctgtgct
     1561 ggctgctggg ttgcctgagc ctcgtgtggc tgacctccgg cgggcccgac gtctacgggt
     1621 tcctgcattt ctccagccac atgatcgccc acatcgggct catgatgatc gccccgccgc
     1681 tgctcaccct ggcggggccc gtgctgctcg cgctgcgtgc cctgccgagc cgtcacgacg
     1741 gcagccgggg gccgcgcgaa tggctgctct ggctcttgca ctccccctac ctggcgttct
     1801 tgtcgaagcc ggtcgtcgcc gggctgatct tcgccggcag cctgtacgcc ttctacttct
     1861 cgccgctttt cccgtgggcc atgcaggacc atacgggcca cctgttcatg accctgcact
     1921 tcctcgcctc cggatacctg ttcttctggg tcttcatggg agtcgacccg gggccgcgca
     1981 cgacgaacta tccggtgctg ctcctgaccc tgctcggcac cgtcgcgttc cacggtttct
     2041 tcggcgtggc gctcatgatg tcgaacggca tcatcgcgcc cgactggtgg cacgccgtcg
     2101 ggctgcagaa tgcggccgcg attctggccg atcagcacac cgccggcggc atcgcctgga
     2161 gcgccgggga gctgccgatg atgctcgcgg cgatcggcct gctctacgcc tggatgaggt
     2221 ctgacgagcg ggcagccgcc aggctcgacc gaaaggccga ccgcgacggc gacgccgacc
     2281 tcgccgccta caacgcgcag ctggaacgca tcgcacagca cgaccacacg cgctgacatg
     2341 cccgcggcgg gcgcagacgg cccccgggga aacggcgccg
//
LOCUS       DASTNL010000038         5029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_954062, whole genome shotgun sequence.
ACCESSION   DASTNL010000038 DASTNL010000000
VERSION     DASTNL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954062"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..468)
                     /locus_tag="VFQ96_01030"
     CDS             complement(262..468)
                     /locus_tag="VFQ96_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0256402.1"
                     /translation="MAALTTLPRPGEHGRRRWCCLTVDLAPTETGTRLRITQQTPGLT
                     DEPRLATIAGWQTFLDVLTEVAEA"
     gene            complement(598..1638)
                     /locus_tag="VFQ96_01035"
     CDS             complement(598..1638)
                     /locus_tag="VFQ96_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU0256403.1"
                     /translation="MRATYMFGAGDVRVVDAPDPRIIEPTDAIVRVTRACICGSDLHP
                     YHSLPPTPGGTTMGHELIGVVEEVGADVVTIKPGDFVIAPFAFSDGTCPFCREGLQTS
                     CEQGSFWNKPERGIGGAQAEAARIPLADGTLVAVPGAVDPSMYAGLLTLSDVYGTGWH
                     AARRAGVTRGDTVAVIGDGAVGLLAVLSAKQLGAERIILLGHHQDRTDLGQEFGATDV
                     VALRGEDGITKVLELTGGYGAHRVLEAVGHLPAYEQALGIVRPGGVISRVGVPQYSQG
                     EVGWPLFGKNATLTGGPAPVRAYIDELLPAVLDGTVQPGKVFDREMSLEETPAGYTAM
                     NERTALKVMIRP"
     gene            complement(1781..2224)
                     /locus_tag="VFQ96_01040"
     CDS             complement(1781..2224)
                     /locus_tag="VFQ96_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HEU0256404.1"
                     /translation="MTAELPWTEPGRRTPYYSRPRDEDLFGVQPTSTAELPDPRPLVE
                     NLSRCVVEAYAGARDIEQLARWVTDRVFQNLLARLALASRARRAKGVTPKRPHFTIGS
                     TRLCAPRDGVVEAVVLIHAPARTRAVAIRLEGLDRRWRATAIHVL"
     gene            2397..3005
                     /locus_tag="VFQ96_01045"
     CDS             2397..3005
                     /locus_tag="VFQ96_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256405.1"
                     /translation="MRWERLFDDLEAQLAETVRAGDDENAREEVRWRAGSLTMRERLL
                     ACRATPAGVRLSGGDRLDVTIHTVGADWFAGVADPAGRAADVVVRTAAVSVLRGASSR
                     GAARVPPVPGREPQPAGSVGGPAQVVSAPRVTLPMVLRDLCRRRAAVTVRGIDGVVHG
                     TIDQVGLDHFDLAVHERGIPRRAANVTAVDILAIAGIMTVEF"
     gene            complement(3149..3451)
                     /locus_tag="VFQ96_01050"
     CDS             complement(3149..3451)
                     /locus_tag="VFQ96_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256406.1"
                     /translation="MSPRTTGLGRFLTPADAAQILAVPLSTVLDLIDSGEIPAIRIGA
                     TGRLRLKRAALDAYIDELYDRRRREMAWHEAGEQKIVEIAGGSVVGRAQGPRLGGI"
     gene            3582..4205
                     /locus_tag="VFQ96_01055"
     CDS             3582..4205
                     /locus_tag="VFQ96_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEU0256407.1"
                     /translation="MAQRSRRIRVRIDPRFLIGIFLVTASVLGVWLFVGRATRGVGIY
                     VAKHTIVVGDPITAGDLATTQVRIGRSVDRYLTPEKLPSEVSYAASSIQAGEFVPRSA
                     VASDPDDGQGRVVVAVAGPLPSTVKVGRSVVVWAVAASSAGDHAQPRVVASRAVVVAV
                     TKREGIVASDTVAVELQVPTDDIAALLEADANANAISLIADRGRVAE"
     gene            4202..>5029
                     /locus_tag="VFQ96_01060"
     CDS             4202..>5029
                     /locus_tag="VFQ96_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256408.1"
                     /translation="MSGIAICAPPRELARLSAALTRHGYRVRARTSLAAGLLAAMTED
                     LPGGGRAVDAVVADADRRCLTAELIGYCDRRGIRVIAVAAGSQGRRRAARLGLFEVVD
                     LAEGEQAVARLVSAPRLGVAPAPASGVLPGLPSSGGMGVPEGALQRSGPEPQGVGRPS
                     NDADLLGVTEAPDDADLAGEAEALGGVDRAERAGRVGQAGLVGGEWGDLGAAEEPPRG
                     EAGRVIAVWGGTGAPGRTTIAVGLAGEYAARGEETALCDVDPYGGSIATLLGLLDEAP
                     "
BASE COUNT          704 a         1715 c         1813 g          797 t
ORIGIN      
        1 atcccttgac cccaccggcc ggggcagggc acgatggatc acccggtgga aacccgccct
       61 gaacgccttc gccatcacct acgaaggccg catcaactaa caatccgcca gaccagaccc
      121 accgttaaac ggacagaccc cgggcaggcc ttccacctcc tgagcgtcag cggccatacg
      181 gcatacgaca agcgccgtcg gcgccgtcga ggcagtagcc gctcgtgacc ggcaccccgc
      241 gccggcgtcc caagggagcc gttaggcctc ggcgacctcg gtgagcacgt cgaggaaggt
      301 ctgccagcca gcgatcgtcg ctaagcgcgg ctcgtcggtg aggcccggtg tctgctgcgt
      361 gatccgcagc cgtgtccccg tctcggtcgg tgcaaggtcc accgtcaggc agcaccatcg
      421 tcgccgacca tgctcgcccg gcctcggcaa ggtagtcaag gctgccacgg ggaacctgca
      481 cgtcacgccc gccgaaccat cgcgcgaatg actcgggagt ggtccacgcg gcaaagactg
      541 ccgcgggcgc cgcggtgacg ccccgatcga tcccgatgcc gtcgtcggtg atgccggtca
      601 tggtcggatc atgactttca gcgcggtgcg ctcgttcatg gcggtgtatc cggcgggcgt
      661 ttcttccagg ctcatctcac ggtcgaagac ttttccgggc tgtactgtgc cgtcgaggac
      721 ggcggggagg agttcgtcga tgtaggcgcg tacgggggcg gggccgccgg tcagtgtcgc
      781 gttcttgccg aagagcggcc agcccacctc gccctgcgag tattggggca ccccgactcg
      841 ggagatgacg ccgccggggc gcacgatgcc gagggcctgt tcgtaggccg gcaggtgtcc
      901 gaccgcttcc agtacgcgat gggcgccgta cccgccggtc agctccagca ccttggtgat
      961 gccgtcctca ccgcgcaggg cgaccacgtc cgtggccccg aactcctggc cgaggtcggt
     1021 gcggtcctgg tggtggccca ggaggatgat ccgttccgcg ccgagttgct tcgctgagag
     1081 aacggccagg aggccgacgg cgccgtcgcc gatgacggcc accgtgtcgc ctcgggtcac
     1141 gccggcgcgt cgggcggcat gccagccggt gccatacacg tcggaaagcg tcaagagccc
     1201 ggcgtacatc gacgggtcga cggcgccggg cacggcgacg agcgtgccgt cggccagcgg
     1261 gatgcgggcc gcttcggcct gggcgccgcc gatgccgcgc tcgggcttgt tccagaacga
     1321 gccctgctcg caggaggttt gcaggccttc gcggcagaac gggcaggtgc cgtccgagaa
     1381 cgcgaacggc gcgatgacga agtcgcccgg cttgatcgtg acgacgtcgg cgccgacctc
     1441 ttcgaccaca ccgatcagct catgccccat cgtcgtgccg cccggggttg ggggcaggga
     1501 atggtagggg tgcaggtcgg agccgcagat acacgcccgg gtcacccgca cgatcgcgtc
     1561 ggtcggctca atgatcctcg ggtctggcgc atcgacgacg cgaacgtcgc cggcaccgaa
     1621 catgtatgtc gctctcacac catccatcct ggcgccagcg gggccctcgc gggtggcttg
     1681 ctcgtcgcgg cgacccggcg agcccggcga tgacgcgcca cgtgcggccg agcccggcca
     1741 tccgtcgcct ggatcgtcta ccgtggccat gcatggtcgt tcagagcacg tgaatggcgg
     1801 tggcacgcca gcgccggtcg agcccttcga gtcgaatggc gaccgcgcgg gttcgggccg
     1861 gcgcgtggat caagacgacg gcctccacga caccgtcgcg gggcgcacac aggcgtgtgc
     1921 tgccgatcgt gaagtgcggc cgtttcggcg tgactccctt ggctcgccgc gcccgagagg
     1981 cgagggcgag acgcgccaga aggttttgga acacgcggtc ggtcacccaa cgggcgagct
     2041 gttcgatgtc tctggccccc gcatacgcct ccacgacaca gcggctcagg ttctcgacca
     2101 acggccgggg gtcgggcaat tccgccgtgc tggtgggttg caccccgaag aggtcttcgt
     2161 cgcgaggccg cgagtaatag ggcgtgcggc gccccggttc ggtccagggc agctccgcgg
     2221 tcatctgcgt tttctcctcc gcgtcatgtg gtgcgcgccc tcggccgggc tgaggagctg
     2281 ccggccaaag tctctattga tagacgaatc aaccagatca cacaggtttt gtccaggtaa
     2341 gaaatcgtgt tgtggataac tgggccggtg cgcgtgatcg tccgtagcct cggtacatgc
     2401 gttgggagcg cctgttcgac gacttggagg cacaactcgc cgagacggtg cgtgcgggcg
     2461 acgacgagaa cgcccgcgaa gaggtgcgct ggcgggccgg ctcgctgacg atgcgcgagc
     2521 gccttctggc ctgccgtgcg accccggcgg gcgtgcgcct ttctggcggg gatcggctcg
     2581 acgtcacgat ccacaccgtt ggcgccgatt ggttcgcggg ggtagccgac ccggcgggcc
     2641 gtgcggcgga tgtggtcgtc cgcacggccg cggtgtcggt gctgcgtggc gcatcgtcgc
     2701 gcggtgccgc acgcgtgccc ccggttccgg ggcgcgagcc gcagcctgcc ggctctgtcg
     2761 gcgggccggc ccaggttgtc tcggcgccgc gggtgactct gccgatggtg ctgcgtgacc
     2821 tgtgtcggcg ccgcgccgcc gttacggtgc gcgggatcga cggggtggtg cacggcacga
     2881 tcgatcaggt cgggctcgac catttcgacc ttgccgtgca cgaacgggga atcccccgcc
     2941 gggcagcgaa cgtgaccgcc gtcgacatcc tcgctatcgc cggcatcatg accgtcgagt
     3001 tctaggcgcg gccgacgcac cttcgtcctg tggccctgtg gctcgtgcgg ctcacagcct
     3061 gctgggcccc gtgatccctg gcctcgtgcg ggtatggtcc tctatgccgc ctctgcccac
     3121 agcggctccc acggtgactc gtgcgccgtc agatgccgcc gagtcggggg ccttgggcgc
     3181 gcccgacgac cgagccgccc gcgatctcga cgatcttctg ctcgccggcc tcgtgccagg
     3241 ccatctcgcg gcggcggcgg tcgtacagct cgtcgatata ggcgtcaagt gccgcgcgtt
     3301 tgagcctcag acggcccgtc gcaccgatgc ggatcgcggg tatctccccg gagtcgatga
     3361 ggtcgagcac cgtcgacaac ggcaccgcca ggatctgcgc ggcgtctgcg ggcgtgagaa
     3421 aacgccccaa accagtcgtg cgcggtgaca tggcttaatt atcgcccgat cgggcacgct
     3481 ctggtctgaa cgtgtggata agtttccggc acgcctgtgc gggaacgtca tcatgggctc
     3541 ggcccaagac gacggcaggg acgacacgag aggagcgccg catggcgcag cgatccagga
     3601 gaatacgggt tcggatcgac ccgaggtttc tcatcggcat ctttctcgtg accgcctccg
     3661 tgttgggtgt ttggctcttc gtcggccgtg cgactcgtgg cgtgggcata tacgtcgcca
     3721 agcacaccat cgtcgtcggg gatcccataa ccgccggcga cctcgccacg acacaggtgc
     3781 gcatcgggcg cagcgtcgat cggtacctca cgccggagaa gctgccgagt gaggtgtcgt
     3841 atgccgccag cagcatccag gccggcgagt tcgtgcctcg ctccgcggtg gcaagcgacc
     3901 cggatgacgg ccagggccgc gtggtcgtgg ccgtcgcggg cccgctgccc tccaccgtca
     3961 aggtcgggcg gagcgtcgtc gtgtgggccg tcgccgcgag ctccgccggc gaccacgcac
     4021 agccccgcgt cgtggcctcg cgggccgtcg tcgtcgcggt cacgaaacgc gagggcatcg
     4081 tcgcctccga cacggtcgcc gtcgaactgc aggtccccac cgacgacatc gcggcgcttc
     4141 tcgaggccga cgccaatgcc aacgcgatca gcttgatcgc agaccggggg agggtggcag
     4201 agtgagcggc atcgcgatct gcgcgccccc acgggagttg gcgcggttgt cggccgccct
     4261 gacgaggcac ggctaccggg tgcgggcgcg aacgtcgctg gcagccggtc ttctggcggc
     4321 gatgaccgag gaccttccgg gcggtggccg tgccgtcgat gccgttgtcg ccgatgccga
     4381 ccggcgctgc ctgacggcgg aactcatcgg gtattgcgac cggcgtggca tccgcgtcat
     4441 cgccgtggcg gccggcagcc agggtcgtcg ccgcgcagcc cgcctcggcc tcttcgaggt
     4501 ggtcgacctg gccgaggggg agcaggccgt ggcacggctg gtttccgcgc ctcgtctcgg
     4561 cgtcgcaccg gctccggcct ccggtgtgct accgggcctg ccttcctccg gcggcatggg
     4621 ggtgccggag ggcgccctgc agcggtcggg tcccgaaccg cagggcgtcg gccgcccgtc
     4681 gaacgacgcc gacctgctgg gagtgaccga agccccggac gacgccgacc tggcgggaga
     4741 agccgaagcc ctcggcggcg tcgaccgtgc ggagcgcgcc ggacgggttg gtcaggccgg
     4801 cctcgtgggc ggcgagtggg gtgacctcgg ggcagctgaa gagcctcctc gaggcgaggc
     4861 gggccgggtc atcgccgtgt ggggcggcac gggtgcgccg ggccgcacca cgatcgccgt
     4921 cggcctggcc ggtgagtatg cggcccgcgg cgaggagacg gcgctgtgcg acgtggaccc
     4981 ctatggcggc agcatcgcga ccctgctggg ccttctcgac gaggcgccc
//
LOCUS       DASTNL010000039         8598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_769518, whole genome shotgun sequence.
ACCESSION   DASTNL010000039 DASTNL010000000
VERSION     DASTNL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769518"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFQ96_01065"
     CDS             <1..486
                     /locus_tag="VFQ96_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU0256409.1"
                     /translation="AAPAPDDADLVFVSSDARLLRFRAAQVRPQGLQASGMSGIALHE
                     GASAIFFGTVGAATLEGALVATVAAGPETLLGAGAGSAKVSLVADFPTKGRATAGVRA
                     QRLLAGEPALALAWVGTAPAHALDAKGRPCALPEAGARRDAAGQPLESAVAFLGGALQ
                     L"
     gene            complement(503..2611)
                     /locus_tag="VFQ96_01070"
     CDS             complement(503..2611)
                     /locus_tag="VFQ96_01070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU0256410.1"
                     /translation="MPDQDYSARQLSVLEGLEAVRKRPGMYIGSTDSRGLLHCLWEIV
                     DNAVDEALAGVGDRIDVILCEDGSVEVRDHGRGIPVDVEPRTGLAGLEVVFTKLHAGG
                     KFGTGSYRSSGGLHGVGASVVNALSERLDVEVDRAGKTWAMSFRRGMPGVFDDAGAPS
                     PDAPFTAAQAPERLRVVGQVPKAVTGTRVRYWADPQVFGTDSALAADLVRARLRQTAF
                     LVPGLALRLQDRRGWQAGAPTVDEAEAETQFRYDGGSSEFVEYLAPDAPVTGVWRITG
                     DGEFDETVPVLGDDGTLTPTLLTRRCEVDVALRWGVGYDTVVRQFANIIETPGGGSHR
                     SGFDAGLLKAIRKQVEKNGRRLKVGRDKLEKDDVFAGLTAVVTVRLPEPQFEGQTKEV
                     LGTPAVRAIVAKTVAAGIRERFASTKKDDKVQTALVLDKVVAEMKSRISARAHRETQR
                     RKTALESASLPAKLVDCRSTDVFDSELFLVEGDSALGTARKARDSEHQALLPLRGKIL
                     NVQKATIADMLGNAECAAIIQVVGAGSGRSFDLAAARYGKVIIMSDADVDGAHIRTLL
                     LTLFFRYMRPMVEDGRVFAAVPPLHRVVVVGRGSAPNETIYTYSERELTAVLADLART
                     KRHYKEPIQRYKGLGEMDADQLATTTMSREHRMLRRVRVADAEKANHVFELLMGHNVA
                     PRKSFIIENSGRLDHERIDM"
     gene            2802..3029
                     /locus_tag="VFQ96_01075"
     CDS             2802..3029
                     /locus_tag="VFQ96_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256411.1"
                     /translation="MEAPEEAAAPNPAEQQLTAFDRCDACGAQAYVRVTINDSELLFC
                     AHHAKEHHAKLAAIATTWHDESDRLLEKQQA"
     gene            complement(3153..4280)
                     /locus_tag="VFQ96_01080"
     CDS             complement(3153..4280)
                     /locus_tag="VFQ96_01080"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0256412.1"
                     /translation="MTGRAAASRPTTAALIDLDALQHNLDVLRRNAAPARFMIVVKAD
                     AYGHGAVPVAHAAVAAGVRDIGALDVPNALALRASGIDEATHVLAWLHTPRQDFTRAV
                     DAGVELTASTVAELDRIATSRSSRTPVVQFKVDTGLHRNGSAVAAWPALMARAARLRD
                     EGLITVKGVWTHIAEASEEEDTKAMRRFDAAIETAAHEGVRFAVRHLAASSAAFRRQD
                     ARYDMVRVGGHSWGIPSFDGITPGEMGLVPVMTLVTGVDAVVRRGSRLVAHLPVGYSD
                     GLPREARGLVHVAIRGRRYLLTEEIRAADTLVPVSADVRAGDLAYLFGTGEHGEETVR
                     QWGDQIGTLGDEIVTRLAPALPRLYLGRAARRLPPGFATLP"
     gene            complement(4277..5377)
                     /locus_tag="VFQ96_01085"
     CDS             complement(4277..5377)
                     /locus_tag="VFQ96_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0256413.1"
                     /translation="MTEETASGRRPGRSGVREALIDVEAIGRNIALGRRASGIDRVLA
                     DVSHNAYGHGAVHAAHAARRAGAAGLLVAGPEEARALREAGILLPIYATRLTGPAGMA
                     LAERYRVVVPVLDAEGLADAAQARVPGVQLMLDIGGDVRGADDWRTLCQAARAGAAPV
                     RGVMAGIDTFDPRAEALLESAIETLHDCGLGAAMLHVCDYEPGHGLEGYRQNTLRMSA
                     ALYGLSAYPEGAAPGLEPALTLTAPLVAVKPVAAGEGVSYGYTYTTSHDTCVLLVPLG
                     YGDGIPRRSGNIARVSVRGVALPVAGRVAMDAMMLDGHALLERGIWPEVGDVATVIGT
                     AGWPAGRWARALQTTSLDIVSRLGPRISRIER"
     gene            complement(5374..6129)
                     /locus_tag="VFQ96_01090"
     CDS             complement(5374..6129)
                     /locus_tag="VFQ96_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256414.1"
                     /translation="MSTPGASALRIVELYSHHLAVNGDMGNTLVLAERARLAGLATVV
                     TRYSPGDDIPEGADLVTMGSGPLSALVAVSPDVERLGRAVRDWAAAGVPMLFVGAGYQ
                     LAGDCVTGLEEPLAGLGVFHQTTDVTSPRAVTASFLVETTFGRLVGVENHGSTTVLTA
                     GQQPFGMALTGRGNGPGHDEGARTNEAIGTHLHGPVLAMNPVLADHMLRMAVVRRGLA
                     YETTREHDRLDGLAAATRELLERGARLGAGRAS"
     gene            complement(6126..7433)
                     /locus_tag="VFQ96_01095"
     CDS             complement(6126..7433)
                     /locus_tag="VFQ96_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEU0256415.1"
                     /translation="MSKTGAGFLLPVLLGRLARLLTRLRGGGSAFPGYVARKVRPGFI
                     ADVASGWPHGVVVILGSNGKSTTTNMVCEILRAHGLRIFTNSSGANMPQGVASALLAE
                     VSLTGRLAADIGVIEVDEAYGAVLCRELRPRAGVILDVQIDQIYRFFEPERVAAMFSD
                     IAGEIEESLVVNRDDDFLGNLGRRLEDEGRLAVSYFGARPEVLAAAPHGLVAAVDYGA
                     EATKGSDVSRPRAVAAEVLSLAGADTVIDIAGERHDLAMPARGLHYAMDSAAALALTK
                     SVLGPAFRPADAIAALAGAKTVYGRGEILPHKDAPIEILLLKNLASLQMNLDYLDETP
                     DSVLFAFDEGSKDPSWLYAADLSHLRHVDVVSGPKAAFVALRLAYAGITFDVVEPDIE
                     KAVAHILATPSPPSGKRTFILDYDQMLLTRKLLGYRDLEAGAA"
     gene            complement(7426..>8598)
                     /locus_tag="VFQ96_01100"
     CDS             complement(7426..>8598)
                     /locus_tag="VFQ96_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0256416.1"
                     /translation="TAKKKAASKTKRAAGTKKSRIDPEDEASRLDEVEDESAADEAQT
                     PEGTQASAGKDKKAAPSLTLPTGALVLSADDEDDVPVHSATITGATADPVKDYLKQIG
                     KVPLLNAAEEVELAMRIEAGLFAEDKLTHSSGLSRELQRELRWVARDGQRAKSHLLGA
                     NLRLVVSLAKRYTGRGMQFLDLIQEGNLGLIRAVEKFDYTKGFKFSTYATWWIRQAIT
                     RAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELSRELDMTPEKVVEVQ
                     KYGREPISLHTPLGEDGDSEFGDLIEDTEAVVPAEAVSFTMLQRQLESLLDSLSEREA
                     GVIRMRFGLGDGMPKTLDQIGDTFGVTRERIRQIESKTMAKLRHPSRSQSLRDYLE"
BASE COUNT         1289 a         3050 c         2957 g         1302 t
ORIGIN      
        1 gccgctcccg cgcccgacga cgcggacctc gtcttcgtct cctccgatgc ccggctgctc
       61 cgcttccgcg ccgcacaggt gcggccgcag gggctccagg cgtcggggat gagcggcatc
      121 gcgctgcacg agggggcgag cgcgatcttc ttcggcacgg tcggcgccgc gacgctcgag
      181 ggggcgctcg tcgccacggt cgcggcgggg ccagaaacac tgctcggcgc gggagccggc
      241 agcgcgaagg tgagcctcgt cgcagacttt ccgacgaagg ggcgcgccac cgccggcgtg
      301 cgcgcacagc gcctcctcgc cggcgagccg gccctcgccc tcgcctgggt gggcaccgct
      361 cccgcgcacg cgctcgacgc gaagggccgg ccgtgcgccc tgccggaggc cggagcccgc
      421 cgcgacgccg cagggcagcc gctggagtcg gccgtcgcct tcttgggggg agcgctgcag
      481 ctctgacgag gcggcggcct cgtcacatgt cgatgcgctc gtggtccagg cggccggaat
      541 tctcgatgat gaagctcttg cggggcgcga cgttgtggcc catcaagagt tcgaagacgt
      601 ggttcgcctt ttccgcgtcg gcgacccgca cccggcggag catgcgatgc tcccgagaca
      661 tcgtcgtcgt cgccagctgg tcggcatcca tctcacccag gcccttgtaa cgctgaatgg
      721 gctccttgta gtgccgtttc gtgcgggcca ggtcggccag cacggcggtc agttcacgtt
      781 ctgaatacgt gtagatcgtc tcgttcgggg cgctgccgcg accgacgacc accacgcggt
      841 gcaagggtgg cacggcggcg aacacccggc cgtcttcgac catcgggcgc atgtagcgaa
      901 agaagagcgt cagaaggagc gtgcggatgt gtgcgccgtc gacatccgcg tcgctcatga
      961 tgatgacctt gccgtagcgg gccgccgcga ggtcgaagct gcgcccgctg cccgcgccga
     1021 ccacctggat gatcgcggcg cattcggcgt tgcccagcat gtcggcgatc gtggccttct
     1081 gcacattcag aatcttgccg cgcagcggca ggagcgcctg atgctcgctg tcgcgcgcct
     1141 tgcgggcggt gccgagcgcc gagtctccct cgacgaggaa gagctcgctg tcgaagacgt
     1201 ctgtcgaacg gcagtcgacg agtttggccg gcaacgacgc gctctccaac gccgtcttac
     1261 gccgctgggt ctcccggtgt gcccgggccg agatgcgcga cttcatctcg gccacgacct
     1321 tgtcgagcac gagggccgtc tggaccttgt cgtctttctt cgtggacgcg aagcgttccc
     1381 tgatgccggc cgcgaccgtc ttcgcgacga tcgcacgcac cgccggggtg cccagcacct
     1441 ctttggtctg cccctcgaac tgcggttccg gcaggcgcac ggtgacgacc gcggtgaggc
     1501 cggcgaaaac atcgtctttc tcaagcttgt cacggccgac cttgagacgt cggccgttct
     1561 tctcgacctg cttgcggatc gccttcagaa gccctgcgtc gaacccgctg cgatggctgc
     1621 cgccacccgg ggtctcgatg atgttggcga actggcgcac caccgtgtcg tagccgaccc
     1681 cccagcgcag cgcgacatcc acctcgcagc gccgcgtgag gagcgtgggt gtgagcgtgc
     1741 catcgtcacc cagcaccggc acggtctcgt cgaactcgcc gtcgcccgtg atacgccaca
     1801 cgcccgtaac cggggcgtcc ggcgccaggt attcgacgaa ctcagaagaa ccgccgtcgt
     1861 agcggaactg cgtctccgcc tccgcctcgt cgacggttgg agcgccggcc tgccagcccc
     1921 gccggtcctg cagacgcagg gcgaggcctg gcacgagaaa cgcggtctgg cggagcctgg
     1981 cacgcacgag gtcggccgcc agcgcggagt cggtgccgaa gacctgcggg tcggcccagt
     2041 agcgcacccg cgttcccgtg actgccttcg gcacctggcc gacgaccctc aagcgttcgg
     2101 gtgcctgcgc ggcagtgaag ggagcatcgg gcgacggcgc gccggcatcg tcgaacacgc
     2161 ccggcatgcc tcggcggaac gacatggccc aggtcttgcc ggcgcggtcg acctcgacgt
     2221 cgaggcgttc cgagagcgcg ttgacgacgg aggcgccgac cccgtgcagc ccgccggagg
     2281 agcggtagga gccggtgccg aacttgccgc cggcgtgcag cttcgtgaag accacctcga
     2341 gccccgcgag gccggtgcgc ggttccacgt cgacggggat gccccgccca tggtcccgca
     2401 cctcgacgct cccgtcctcg cagaggatca catcgatccg gtcgccgacg cctgccaagg
     2461 cctcgtcgac ggcgttgtcc acgatctccc acaggcagtg caggagcccg cgggagtcgg
     2521 tcgacccgat gtacatgccg gggcgtttgc gcaccgcctc gagtccttcg agcaccgaaa
     2581 gctgcctggc tgaatagtcc tggtcgggca cggatccctt tcttctcgcc gcagacgaac
     2641 ggcgccggtc tcagcctagg gggtcgcgca gccggctacg cggtgagcga aaatagccgc
     2701 ccccaccggg ccagggcccg aggacgtgct tgactggagg cagctacgaa gcccgacgtc
     2761 gctgggcgtg aggaggagag acgatgccac acactcgatc cgtggaagct ccggaggaag
     2821 cggcggcccc gaacccggcc gaacagcagt tgaccgcctt cgatcgctgc gacgcatgcg
     2881 gcgcccaggc ctatgttcgg gtgaccatca acgacagtga gctgctcttc tgcgcccatc
     2941 acgcgaagga gcaccacgcc aagctcgcgg cgatcgcgac cacctggcac gacgagtctg
     3001 accggctgct ggagaagcag caagcctgac gtgatgctcc cggcccagac cgagggcgac
     3061 gaggcgacac gaacgcccgt cggcggatcc tccagggtct tgccgacggg cgtttctcgt
     3121 cgggggcgtg ccggcggccg cgccgcccgc ggtcacggca acgtcgcgaa ccccggcggc
     3181 agccgccggg ccgcacggcc gaggtagagg cggggcagtg ccggcgccag ccgggtgacg
     3241 atctcgtcgc ccagggtacc gatctggtcg ccccactgac gcaccgtctc ttcgccgtgc
     3301 tcccccgtgc cgaagaggta ggcaagatcg cccgcccgaa cgtcggcgga gacgggcacc
     3361 agcgtgtcgg ccgcgcggat ctcttcggtg agaaggtacc gccggccccg aatggcgacg
     3421 tgcacgagcc cccgcgcctc cctcggcaag ccgtcggaat agcccaccgg caggtgggcg
     3481 acgaggcgcg acccccggcg cacgacggcg tcgacgcccg tcacgagggt catcaccggg
     3541 acgagcccca tctcgcccgg cgttatcccg tcgaacgagg gtatgcccca cgaatgcccg
     3601 cccacgcgca ccatgtcgta gcgggcgtcc tggcgccgaa aagccgcaga gcttgccgcc
     3661 agatgccgca ccgcgaacct cacgccctcg tgtgcggccg tctcgatcgc cgcgtcgaaa
     3721 cggcgcatcg ccttcgtgtc ttcctcttcg gaggcctccg cgatgtgggt ccacaccccc
     3781 ttgacggtga tgagaccctc gtcgcgcaac cgggcggccc gggccatgag cgccggccag
     3841 gccgccaccg cggagccgtt gcggtgcaga ccggtgtcca ccttgaactg gacgacgggc
     3901 gtgcgggagg agcggctggt ggcgatgcgg tcgagttccg cgacggtgga ggcggtcaac
     3961 tcgacgccgg catcgacggc ccgggtgaag tcttgccggg gtgtgtgcag ccatgcgagc
     4021 acatgggtcg cctcgtcgat gccggaggcc cgtagggcga gcgcattggg cacgtcgagg
     4081 gcgccgatgt cacgcacccc ggccgcgacg gcggcgtgcg cgaccgggac ggcgccgtgc
     4141 ccgtaggcgt cggccttgac gacgatcatg aacctcgcgg gcgccgcgtt tcgtcgcagc
     4201 acgtcgaggt tgtgctgcag cgcgtccagg tcgatgagcg ccgccgtcgt cggccgggag
     4261 gcggccgccc ggccggtcat cgttcgatcc tcgaaatacg cgggcccagc cgggagacga
     4321 tgtcgaggct cgtggtctga agcgcccggg cccagcgccc ggccggccag ccggccgtgc
     4381 cgatcaccgt cgcgacatcg cccacctcgg gccagatgcc ccgttcgaga agggcgtggc
     4441 cgtcgagcat catggcatcc atcgcaaccc ggccggcgac cggcagggcg acgccgcgca
     4501 cgctgacccg ggcgatgttg ccgcttcgac gcggaatgcc gtcgccgtat cccagcggga
     4561 cgagcagcac gcaggtgtcg tgtgacgtgg tgtacgtgta cccgtaagag accccctcgc
     4621 cggccgcgac cggcttcacc gcgacgagcg gcgcggtgag cgtcagcgcc ggctccagac
     4681 ccggtgctgc gccctccgga taggcgctca ggccgtagag tgccgcggac atccggaggg
     4741 tgttctgccg atacccctcc agaccgtggc cgggttcgta gtcgcacacg tggagcatcg
     4801 ccgcaccgag gccgcaatcg tgcagggtct cgatcgccga ctcgagcagc gcctcggccc
     4861 gcgggtcgaa ggtgtcgatg ccggccatca cgccccgcac cggcgcggcg ccggcgcgcg
     4921 ccgcctggca gagggtccgc cagtcgtcgg cgccgcgcac gtcgcccccg atgtcgagca
     4981 tcaactgcac acccggcacc cgggcctgcg cggcgtcggc cagcccttcg gcgtcgagga
     5041 ccggcacgac gacccggtat cgctcggcga gggccatgcc ggccggcccg gtgagacgcg
     5101 tggcatagat cggcaggaga atccccgcct cccgcagcgc ccgggcctcc tccggccccg
     5161 ccacgagcag ccccgcagca cccgcgcgcc gggccgcatg cgcggcatgc accgcgccat
     5221 gcccgtaagc gttgtgagag acatcggcca acacccgatc gatgcccgac gcccggcggc
     5281 cgagcgcgat gttccggccg atcgcctcga cgtcgatcag cgcctcgcga accccggagc
     5341 gccccggccg cctccccgag gcggtttctt cggtcacgag gcccggccgg cgccgagcct
     5401 cgcgccccgc tccaagagtt cccgggtcgc ggcagcaagc ccgtcgagac ggtcgtgctc
     5461 ccgggtggtc tcgtaggcga ggccgcgtct taccacggcc atccgcagca tgtgatcggc
     5521 gagcaccggg ttcatagcaa gcaccgggcc gtgcaggtgc gtgccgatcg cctcgttcgt
     5581 acgggcgcct tcgtcgtgcc ccgggccgtt gccgcggccc gtcagcgcca tgccgaacgg
     5641 ttgctgcccg gcagtgagca ctgtggtcga cccgtgattc tcgacaccca ccaggcgccc
     5701 gaacgtcgtc tcgacgagga acgaggcggt gaccgcccgg gggctcgtga catcggtcgt
     5761 ctggtggaac acgcccagcc cggccagcgg ctcttcgagg ccggtcacgc aatccccggc
     5821 gagttgatag ccggctccga cgaacagcat gggcacgccc gccgcggccc agtcgcgcac
     5881 cgctcggccg agacgttcga cgtcgggaga gacggccacg agcgcggaga gcggcccgga
     5941 gcccatcgtg acgaggtcgg ccccctcggg gatgtcgtct cccggggaat atcgggtcac
     6001 gaccgtcgcg aggcccgcca gccgcgctcg ctcggcgagg accagcgtgt tgcccatatc
     6061 gccgttcacg gcgaggtggt gtgaatacag ttcgacgatc cgcaacgcgg aggcacccgg
     6121 ggtactcatg ccgcacccgc ctccaagtcg cgatagccca gcagcttgcg cgtcagcagc
     6181 atctggtcgt agtcgagaat gaaggtgcgt ttgccgctcg gcggcgacgg cgtggcgagg
     6241 atgtgtgcca cggccttctc gatgtcgggc tccaccacgt cgaacgtgat gcccgcgtag
     6301 gcgagccgca aggctacgaa ggccgccttc gggcccgaga cgacgtccac atgccgcaag
     6361 tgagagaggt cggcggcgta gagccaggag ggatccttgc tgccttcgtc aaaggcgaac
     6421 aacaccgagt ccggggtctc gtcgagatag tcgagattca tctgaaggct ggcaaggttc
     6481 ttcaaaagca ggatctcgat cggcgcgtct ttatgcggca ggatctcgcc ccgcccgtac
     6541 acggtcttcg ccccggccag cgcggcaata gcgtcggccg gccggaacgc cggcccgagc
     6601 acgctcttcg tcagggcgag cgccgcggcg ctgtccatgg cgtaatgcag cccccgggcc
     6661 ggcatcgcca gatcgtgccg ctcccccgcg atgtcgatga ccgtgtcggc cccggcgagg
     6721 ctcaacacct cggcggcgac cgcgcgcggc cgagagacgt cggagccctt cgtcgcctcg
     6781 gcgccgtagt cgacggcggc cacgaggccg tgcggcgcgg ccgccaacac ctcgggccgc
     6841 gcgccgaaat acgagaccgc gaggcgcccc tcgtcctcga gccggcgccc aagattgccg
     6901 aggaaatcgt cgtcgcgatt caccacgagt gattcttcga tctcgccggc gatgtcggag
     6961 aacatagcgg cgacccgctc cggctcgaag aagcggtaga tctggtcgat ctgcacgtcg
     7021 aggatgacgc cggctcgcgg ccgcaattcg cggcagagca ccgcaccgta ggcctcgtcg
     7081 acctcgatga cgccgatatc agccgccagg cgcccggtga ggctgacctc ggccagaagc
     7141 gccgaggcga cgccctgcgg catgttcgcg ccggaagagt tcgtgaagat gcgcagaccg
     7201 tgcgcccgca gaatctcgca gaccatgttc gtggtcgtcg acttgccgtt cgaaccgagg
     7261 atcacgacca cgccatgcgg ccacccggag gcgacgtcgg cgatgaagcc ggggcgcacc
     7321 ttgcgggcga cgtagcccgg aaaggccgag ccgccgcccc gcagtcgcgt gagaagccgc
     7381 gccagcctgc cgagaagaac cggtagcaga aagcctgcgc cggtcttact caaggtagtc
     7441 ccgcagtgac tgcgaacgcg acgggtgccg cagcttcgcc atcgtcttcg actcgatctg
     7501 gcgaatccgt tcccgcgtga cgccgaacgt gtcgccgatc tggtcgaggg tcttcggcat
     7561 gccatcgccg agcccgaagc gcatccggat gacgccggcc tcacgctccg agagggagtc
     7621 gagcagactc tccagctgcc gctgcagcat ggtgaagctg accgcctcgg ccggcacgac
     7681 cgcctcggtg tcttcgatga ggtcgccgaa ctcgctgtca ccgtcctctc cgagcggggt
     7741 gtgcagcgag atcggctcgc ggccgtactt ctgcacctcg acgaccttct ccggggtcat
     7801 gtcgagttcc cgcgagagtt cttcgggggt gggctcacgc ccgaggtctt gcaacatctg
     7861 gcgttgcacc ctggcgagct tgttgatgac ctccaccata tggacgggga tgcggatggt
     7921 tctcgcctgg tcggccatcg cccgcgtgat cgcctggcgg atccaccagg tggcgtaggt
     7981 ggagaacttg aagcccttgg tgtagtcgaa cttctcgacc gcgcgaatga ggccgaggtt
     8041 gccctcttga atgaggtcga ggaactgcat gccccgcccc gtatacctct tggccagcga
     8101 cacgacgagg cggaggttcg cgcccagaag atggcttttg gcccgttgcc cgtctcgcgc
     8161 gacccatcgc agctcccgct gaagctccct ggacagcccg ctggaatgcg tcagtttgtc
     8221 ttcggcgaaa aggccggcct cgatgcgcat cgcaagctcg acctcttcgg ccgcgttcag
     8281 caagggaacc ttgccgatct gcttgaggta gtccttcacg gggtcggcgg tcgcgccggt
     8341 gatcgtcgcc gagtgcaccg gcacgtcatc ttcgtcgtcg gcgctcaaga cgagggcgcc
     8401 ggtaggcagg gtgaggctgg gcgccgcctt cttgtccttg ccggcgctcg cctgcgtacc
     8461 ctcgggcgtc tgggcctcgt cggccgctga ctcatcctcg acctcgtcga gacgcgacgc
     8521 ctcgtcttcg gggtcgatac gggacttttt cgtgccggcg gcccgtttgg tcttcgaggc
     8581 cgccttcttt ttcgccgt
//
LOCUS       DASTNL010000040        17921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_584668, whole genome shotgun sequence.
ACCESSION   DASTNL010000040 DASTNL010000000
VERSION     DASTNL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 17921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17921
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584668"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..154
                     /locus_tag="VFQ96_01105"
     CDS             2..154
                     /locus_tag="VFQ96_01105"
                     /inference="COORDINATES: protein motif:HMM:NF022717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 family protein"
                     /protein_id="HEU0256417.1"
                     /translation="MKSYSPEVDYGALERELAAKSHTEDNDYAEWAAHGADEDYSDYS
                     SQNKRA"
     gene            234..908
                     /locus_tag="VFQ96_01110"
     CDS             234..908
                     /locus_tag="VFQ96_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEU0256418.1"
                     /translation="MSTEEPAGSESRDASAEGGAALPPAGWYPDPQGPGSRWWDGEHW
                     TDQYLRQPPAGRAPGTLGGYDGQQAPGYGPYRDDGRGAWSAGRAPIGAWRSAVDDRPP
                     VSGIGGATRAVFGKYAQFAGRAGRPEFWFWVLFCAIVNVAAFIVFLLLDVLAVAVGSV
                     PIVFAGIIGVALLLWGLAIIVPTVAVTVRRLRDGGFHWALLFLQLVPGGGIVILILCC
                     LPSKYE"
     gene            complement(969..2936)
                     /gene="acs"
                     /locus_tag="VFQ96_01115"
     CDS             complement(969..2936)
                     /gene="acs"
                     /locus_tag="VFQ96_01115"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU0256419.1"
                     /translation="MADAHQNIDALMDEERRYRPDPAFAAQANAQPGFYDQDPDAFWE
                     REGRERLSWFTPFTQLRQWDRPYAKWYLGGRLNACYNCVDRHVAEGRGDKVAYFWEGE
                     PVDERLAITYADLQRRVVKAANAFRSLGVRKGTKVAVYMGMVPEAPVTLLALARIGAP
                     FTFVFGGFSATALGSRMNDMRCEFLVTQDAAWRRGKPSPLKPIADEAMAMAPSVTTSI
                     TVRRTGTPVEMTAGRDVWWHDVVDGQSDDAASCPCEPMDSEDMFFLMYSSGTTGRPKG
                     IVHTTAGYLAGVSTTHHYVFDIKPETVYWCAADIGWITGHSYIVFGPLVNGTTSVMYE
                     GTPDFPDKDRWWQIVERYKVSVLYTAPTTIRTHMKWGSEYADRHDLSSLKVLGSVGEP
                     INPEAWVWYRDHVGGGKAPIMDTWWQTENGMILVSPLPGLTTLKPGAATHPFIGVEAA
                     VYDEDGNEIPAGSGAGYMVVKKPWPAMFRGLYGEDERFREAYWSKFPDAYLAGDGARV
                     DADGDFWLMGRIDDVMNVSGHRLSTIEIESALVDHPDVAQAAVVGRSDPTTGKAIVAY
                     VTLKDGAHGTTAKLAELRDHVGKVIGPIAKPANIVFTPELPMTRSGKIMRRLLKDVAE
                     NRQLGDTTTLADPSVVDEIKRRSEAEAAQED"
     gene            complement(3098..3220)
                     /locus_tag="VFQ96_01120"
     CDS             complement(3098..3220)
                     /locus_tag="VFQ96_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256420.1"
                     /translation="MTGAHLASLAASKPEGRLATLDKGLAEALSDMTELLPRPA"
     gene            complement(3257..4351)
                     /gene="purM"
                     /locus_tag="VFQ96_01125"
     CDS             complement(3257..4351)
                     /gene="purM"
                     /locus_tag="VFQ96_01125"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU0256421.1"
                     /translation="MASASYRAAGVDTAAGDLAVELMRDAVARTHGPQVVGGVGGFAG
                     LFDARALRDMRRPLLATSTDGVGTKLALAQALDKHDTVGQDLVGMVVDDIVVVGARPL
                     FLTDYIACGRVVPARIATLVAGIARACEETGTALVGGETAEHPGVMGPEEYDLAGAAT
                     GVVEADALLSPERVRGGDAVLALASSGLHSNGFSLVRRILADRGVALTSGLAGLGEVG
                     EELLTPTRLYTAPLLAALDAAGGAIHALSHVTGGGMAANLARVLPQGSWAEIDRSTWS
                     PQPVFRVLCDLGGLRLADVEDTWNLGVGFFALVAPEAAEGVRRGLVSSGIPTWQAGVV
                     RQGPRDLAGFVQGAKGVAGGAVRLVGDYAA"
     gene            complement(4373..5227)
                     /locus_tag="VFQ96_01130"
     CDS             complement(4373..5227)
                     /locus_tag="VFQ96_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256422.1"
                     /translation="MTSLLNRGAVHDLKPDESFVHLLDDADERERMMARGDEAGTAPA
                     TAVAVPEAAPAAKAPGLAGPRHERLPGSGRGADSFGEVFEQLLDERERVAAASAAREA
                     RAENPRPAAGRALASDRAEYGLPPAPTLAAGPGDLVFLFGMRDDAWAAAQALAVAHPG
                     MQLGVAGTLARPGFRHVGGRREATQARADGVRTGAGTLVAVGVGLAPNDAEQYADVLG
                     EVTPDQVWIAVDVTRKPEDTRLWAAAVARAAGGIDAAAVIGTAYTSTPGSTRLLGIPV
                     GWSDGVLS"
     gene            complement(5154..7268)
                     /locus_tag="VFQ96_01135"
     CDS             complement(5154..7268)
                     /locus_tag="VFQ96_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEU0256423.1"
                     /translation="MRRKNLSLVAVPVGVVGIVLLLVVPVPAWLLDVLITINIVFALV
                     ILLTTMFTKRPLDFSVFPSVLLIATLLRLGLNVASTRLVLGTGFAGKVIETFGTVAVG
                     DNIIIGAVVFLILVVIQFVVVTKGAERVAEVGARFTLDAMPGKQMAIDADLNAGLITD
                     TEAKRRRAEVAMEADFYGAMDGASKFVKGDAIAGILIIVINVIGGIGIGMLQGGLSIT
                     DALHKYTILTIGDGLTAQIPALLMAVSTGMIVTRSGAESDMGSTAGKQLAQSRSALLI
                     AGCAAVALALIPGMPKLSFFTIGGLLIWAARRIAFHAARQQAAAASKAAAEKATGVSD
                     TTDDLIAQMRVHPLEIQLASDLIDIVSGGSDDLLGRVRALRRKLAVDLGIVVPPVRTR
                     DNSELPPATYAILVAGVEAGRGTAPRGKVLTLGAGLGTLPGEETVEPVFGLAAKWIPV
                     ELRHNAEMAGATVIDRVSVVVTHLSQVVSLNAARLLGREDVRLLTDGVRRVNPSAVDE
                     LTPALLSLAEVQRVLQGLLAERVAINDLGRIYEAVTLRAKASTEPEGLIEAARAALGA
                     ALAAPYLDDGVLRVLTFEPVLEQSLLEGLVQRESGSRIVLEPDRLERLIAAIRTTTAR
                     HDQAVLVCAPQLRPAVRRLVVQQAPETAVLSYTEATAARATIETIGVIRVDTSTMVVD
                     QPAEPGGGARPQTRRVVRSPVG"
     gene            complement(7265..8455)
                     /locus_tag="VFQ96_01140"
     CDS             complement(7265..8455)
                     /locus_tag="VFQ96_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEU0256424.1"
                     /translation="MAGEAQERTEQATEKHLKEARKKGHLQKSRDLVAWTSVAAGVVM
                     LPTTLHSAYTDLGAQVASVGDVVADPSLAAVRSSLYDGLGTLGHIMLPMLLAVGVTAV
                     AAGVVQGGVHLRRLAPMPGQFNLVKGLGRIVGPRAAWEAAKTLVKALAVGLGFALVIR
                     AWLPTLLQTGGMPLGGLVQAGSSSAAMMLLVVVGAGLAFAGLDLMVVLRRNRASTRMT
                     KREVKDELKNTDGDPLVRSHRRSMQLAMSRNRMIAAVGHANVVIVNPTHVAVALAYQP
                     GQQAPRLVAKGADFIAARIREEARQKQVPIVEDIPLARALYGACEVGAQIPVEFFVPV
                     ANVLAFLMLVKRRGASLGDVLTVPAQPAATGRHRQGGAATQPAPVRAATAAATPSKET
                     GVSV"
     gene            complement(8455..9210)
                     /locus_tag="VFQ96_01145"
     CDS             complement(8455..9210)
                     /locus_tag="VFQ96_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEU0256425.1"
                     /translation="MNVTFDPATVSAIALIAVRIAAFLAIAPPFSSTAFPLQLKAALS
                     VLLALVLVPRVAAGYTEQDTGPFLLALVFAAVTGAALGFLVYIAFAAVQVSGSFLDQF
                     GGFAAAEQYDPGAGTNGAQFTRLYQMTALALLFASDGYQLILGGLVRSFTAIPIAAHV
                     DIDRVAQSLAGSVTQLFVSALQIAGPTIVVLFLANVALGLLTRVSPAMNAFTLGPPAF
                     TLVTVLLTGFGIVALPAAVNALAASAGQLIGGL"
     gene            complement(9213..9506)
                     /gene="fliQ"
                     /locus_tag="VFQ96_01150"
     CDS             complement(9213..9506)
                     /gene="fliQ"
                     /locus_tag="VFQ96_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEU0256426.1"
                     /translation="MDSAAVMNIAAQALFETAKLCAPIVLTALVVGFAVSLLQSVTQI
                     QEVTLSFVPKAVAVAIALLVSGSWMISEIVTFTQHLIGQIPELIGQGTMLIGG"
     gene            complement(9515..10198)
                     /gene="fliP"
                     /locus_tag="VFQ96_01155"
     CDS             complement(9515..10198)
                     /gene="fliP"
                     /locus_tag="VFQ96_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592421.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEU0256427.1"
                     /translation="MIVAAQVAAATISINGPNGKPADAIVTLLGITLLSVAPALLIMM
                     TSFTKVFVVLAMARNALGTQTIPPNQVLAGLALFLSLFIMEPTLAAIYHHAVEPYLSG
                     GIDFTQALHAAEVPLRAFMGAHTRQEDIALITRAAGRANPASPAAVPFLTLVPAFVLS
                     ELRTAFIIGFVLFIPFLIIDLVVAAGLMSLGMMMLPPVMISLPFKILLFVLVDGWGLV
                     IRSLISSYT"
     gene            complement(10195..10701)
                     /locus_tag="VFQ96_01160"
     CDS             complement(10195..10701)
                     /locus_tag="VFQ96_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEU0256428.1"
                     /translation="MDTLLLALRVLVALGAVVALLWVLQRRSLRWAAKHHSGTTIEVA
                     GKRSLGSKASVVVVDVDGTRFTLGVTEHSVNVLHSSRVPAQAEAGEAPLAPRAVPPLR
                     VAGGADDEAATAAGVAGRSADAARHVRAEDSHPVYDFARMLGRAESSAVFSRSQWRPL
                     AKTRKSRH"
     gene            complement(10704..11387)
                     /gene="fliN"
                     /locus_tag="VFQ96_01165"
     CDS             complement(10704..11387)
                     /gene="fliN"
                     /locus_tag="VFQ96_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEU0256429.1"
                     /translation="MTLNAYEKAAQTLAHAPLFSGPLTVAARPAGAPVPSAAEHAVTA
                     RFVGASSSELVVALADGYDLGGASDRLGPADLLRPLLATAAAELGPGTLSDASVVDAR
                     DVFADPETDSFAVVDESGGEIAWFAARTLRASAPARAAAADVTGRLSRISNVEMALTV
                     RIGYTRMAVRDVLAIEPGAVIELDRSAGSPADILLNGRLIAHGEIVVVDQDYAVRVTQ
                     ILDPAEDVA"
     gene            complement(11384..12268)
                     /locus_tag="VFQ96_01170"
     CDS             complement(11384..12268)
                     /locus_tag="VFQ96_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEU0256430.1"
                     /translation="MSAVAMDRAGEARLYDFRRPTTLVREHSRTLELAFDTFARQWGT
                     QLTAKIRVLSQVTFQSVRMLGYDEYASGLPATTAMILLGLSDVEPRAVLQFPTPSALT
                     WINSMLGGTRPVQPVERTFTPIEYALVRHITDDALEDLKYSLGPLLPGSIRVDSIQYH
                     SQFAQAAPTTELMVIARFTLRVGDNEAEASLALPWQVLLPQLGEANPTVPSDDATHYL
                     RRQLVQVPIEVAASLDPAYVTPSEVLSLKVGDVLRLPHPTHHPLNLVTGEQRVASVAI
                     GTSRSRLAVVVTDLEEGA"
     gene            complement(12265..13164)
                     /locus_tag="VFQ96_01175"
     CDS             complement(12265..13164)
                     /locus_tag="VFQ96_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEU0256431.1"
                     /translation="MTRHRPGRARRGASTDDEAAHGAGEERWMASYLDMVTVLMCTFL
                     VMFAMSSVNANKFKELKNSLQTGFGIVKTQKVDTAHGVVVPPKDVNDPKATHPSPVAL
                     AKVELDKLEALKAAINKSLTEKGLEKDVSYTIDQRGLTIGLIGNKTFFHTNLADLTPT
                     AERVIAAIAPIIDTVDNKVSLEGHADGRPPLPPYPTNWELAAARSIAVLRSLNGDGVP
                     ASRLDAVSYGDVQAKEGGDSAQDMADERRVDMVILSGQSEDVRMLIPKLLREEQGAGP
                     AAPPAGTAQGAPAGGLGQEVPAA"
     gene            complement(13161..14030)
                     /locus_tag="VFQ96_01180"
     CDS             complement(13161..14030)
                     /locus_tag="VFQ96_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0256432.1"
                     /translation="MDLATIVGLLAAFGSLAATIFIEGSDPSSLLLPGPLILVFGGTI
                     GVGIAGATMHDTIAAFKLVPAAFRGRRPDMAGTIAALVRLADLVRHDGLLALDSAAEE
                     IDDPFLKTAMQGVADGADSDDLATRLQDEIDTRAAGLRRGAKFFGVLGGYAPTVGIIG
                     TVVSLTHVLAHLSSPNALGPMIASAFIATLWGLLSANFIWLPIGSRIGRLCDLEVERQ
                     NLVLEGVLAIQAGVSSRALAERLAVLVPAETGKGGDKEKKDKDGKGGEAPERGAKAKG
                     RGTKAERKESVNA"
     gene            complement(14033..14272)
                     /locus_tag="VFQ96_01185"
     CDS             complement(14033..14272)
                     /locus_tag="VFQ96_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEU0256433.1"
                     /translation="MILVTRLNGGRLAVNPDLIERITASPDSTITMVDGVCYVVRESL
                     ETVIDRIVDYRARVLAVASSKDGGGDSLLTMIGGE"
     gene            complement(14325..15539)
                     /locus_tag="VFQ96_01190"
     CDS             complement(14325..15539)
                     /locus_tag="VFQ96_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEU0256434.1"
                     /translation="MLRSLDSGVSGLQAQQTMLDVVANNIANVNTTGYKATSVEFEDT
                     LSQVLHNASGPNANNGGTNPAQVGLGVEVAGIATDFTGGATQTTGQGTNMMINGNGFF
                     IVNNGGQTAYTRAGAFTLDGAGDLVAPDGSLVQGWSANGTGQVQRGAAPGTLHIPLGS
                     MVPATATTSITMSGNLPEDATTTPTPTTLTSQAQVYDSAGKPHSITLTYTFGGVDGSN
                     NATWNVAATDETGASLGTGTVVFGSDGSLTSGGTMSLSDGITVDMSKLTGYAGTTSVN
                     VADQNGNAAGTLQSFTIGTDGTIEGAFSNGAKQAIGQVAIATFSNPSGLEKLGDSLFS
                     STANSGLAQVGAPGSGGSGTISADAVEGSNVDLGREFTNLIVAQRAFQASARVITTSD
                     DVLQELVNLKTN"
     gene            complement(15638..16108)
                     /locus_tag="VFQ96_01195"
     CDS             complement(15638..16108)
                     /locus_tag="VFQ96_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEU0256435.1"
                     /translation="MSALAPLGAPTPATAGSSVPVTSAGAPPAPAADDAQGQSIDYMS
                     LLLAQLQNQDPSSPMDATQMVAQTTQLGMLQQLTQMESDANQALTFQMRQTAASLVGL
                     TVQYTDANGASATGVVQGVTFGTTTPTVTIGGVSLPLSSVAGVTSGAAAAGSTA"
     gene            complement(16144..>17921)
                     /locus_tag="VFQ96_01200"
     CDS             complement(16144..>17921)
                     /locus_tag="VFQ96_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEU0256436.1"
                     /translation="RPAAQPGGTGGDLFAQILHAAADPGAGQARPASAAVRPNGPGPE
                     HPGRGARLAAGGRDLAGPGASSARARSSSQHEKDDGGPGAACTAALGFAALSAAAPAQ
                     AGSAQAGPATQPGPTTQAGSAARAGSVAQDASAARVGPAVQAALAAAAAAPAGPAGAP
                     GTASLAAQGMTASSAAAAHVAAPGPGVATLAATMLGAGGSAAAGVSAGGRAVPGLSGV
                     AAGPFAAVQGTVGEASAVDSAVGGKAGPGAGVPSSPVTGTAIAGASTLLPQAGVAGTP
                     RAAVAGGGYAGVLASGAAGAGTASASRATTATASAALSGAVSQAGVAMPAGRAGAGGG
                     MEQSGRGLPGAPQPQGPGRLAGRAAAQTGTAAPRASVSGAQALASQAPATAVRPSPGP
                     GDGGSSKSASGDASGPAPAPASAAAQALGAAAPSSQNALVGVAPTPAQAALSPLAGGL
                     LPQLVPQIAALAAQGSGMHTLQLRVTPENLGTVTVTVDVTEQGVHVQLFSSTDAGQNA
                     LRQLLPQLHHDLGQTVSGGFLTLSDPSGSGADSGGQAAGGQGQTATWGPPQDGARLTD
                     ERRQDSEHPVRRPLPGSTSVLDIFA"
BASE COUNT         2684 a         6066 c         6527 g         2644 t
ORIGIN      
        1 gttgaagtcg tacagtcctg aggtagacta cggcgcgctg gagcgggagc tcgcggccaa
       61 gtctcatacg gaagacaacg actacgccga atgggccgcg cacggcgccg acgaggatta
      121 cagcgactac tcgtcgcaga acaagcgcgc ctgaacgctc cggcagcccg accccactcc
      181 gccaagaggc cgaccgcacc gcggaaccgg cagcgagcat cgaggagccc atcgtgagca
      241 ccgaggaacc cgccggcagc gaatcgcgtg acgcctccgc cgagggtggc gcagcgctgc
      301 cacctgccgg ctggtacccc gacccgcaag gcccgggctc acggtggtgg gatggcgagc
      361 attggacgga ccaatacctt cggcagccgc ccgccggccg agcgcccggc accttaggcg
      421 ggtacgacgg gcagcaggcg ccgggctacg gtccgtaccg cgacgacgga cgcggcgcgt
      481 ggtcggctgg ccgtgccccg atcggggcat ggcgttcagc cgtcgacgac cggcccccgg
      541 tgagcggcat cggcggcgcg acacgggccg ttttcggcaa gtatgcgcag ttcgccggcc
      601 gggccgggcg gccggagttc tggttctggg tgctcttctg cgccatcgtc aacgtggccg
      661 cgttcatcgt ctttctgctc ctcgacgtgc tcgccgtcgc ggtgggctcc gtgccgattg
      721 tcttcgcggg gatcatcggc gtcgctctgc tcctgtgggg gctggcgatc atcgtgccga
      781 ccgtcgcggt gacggtgcgt cggctgcgcg acggcggatt ccactgggcc ctgctcttct
      841 tacagctcgt gccgggtggc ggcatcgtca tcctgatctt gtgctgcctg cccagcaaat
      901 acgagtgagc atcgggcggg agaaggaccg ggccgcggag gcgaccacac aggccgcccc
      961 cgcggcgctc agtcttcttg agccgcctcc gcctcggagc ggcgcttgat ctcgtcgacc
     1021 acgctcgggt cggcgagcgt cgtcgtgtcg cccaactgcc ggttctctgc cacgtctttc
     1081 aagagccggc gcatgatctt gcccgaccgc gtcatcggaa gttccggcgt gaagacgatg
     1141 ttcgcgggct tcgcaatcgg cccgatcacc ttgcccacgt gatcccgcag ctcggccagc
     1201 ttcgcggtcg tgccgtgagc accgtctttc aacgtgacgt aggcgacgat cgccttgccg
     1261 gtcgtcgggt cggagcggcc gaccacggcc gcctgcgcga cgtcgggatg atcgaccagc
     1321 gcgctctcga tctcgatggt cgacagacgg tgcccggaga cgttcatgac gtcatcgatg
     1381 cggcccatca gccagaagtc gccgtcggca tccacccggg ccccgtcgcc cgcaagatac
     1441 gcgtcgggga actttgacca atacgcctcc cggaaccgct cgtcttcgcc gtagagcccc
     1501 cggaacatcg ccggccacgg cttcttcacc accatgtatc cggcgccgga accagccggg
     1561 atctcgttgc cgtcttcgtc gtagacggcc gcctccacgc cgatgaaggg gtgggtggcc
     1621 gcaccgggct tcaaggtcgt cagccccggc agcgggctca cgaggatcat gccgttctcc
     1681 gtctgccacc acgtgtccat gatcggagcc ttgccgccgc cgacgtggtc gcgataccag
     1741 acccaggcct ccggattgat cggctccccc acggagccca gaaccttcag cgacgacagg
     1801 tcgtgccggt cggcgtattc cgagccccac ttcatgtggg tgcggatcgt cgtgggcgcc
     1861 gtgtagagca cgctcacctt gtagcgctcc acgatctgcc accagcggtc cttgtcggga
     1921 aagtcgggcg tgccttcgta catgacgctc gtcgtgccgt tgacgagcgg gccgaagacg
     1981 atgtagctgt gcccggtgat ccagccgatg tctgccgcgc accaatacac cgtctccggt
     2041 ttgatgtcga agacgtagtg atgggtcgtg gacaccccgg ccaggtaccc cgccgtcgta
     2101 tgcacgatgc ccttcgggcg gcccgtcgtg ccgctggagt acatgaggaa gaacatgtct
     2161 tccgaatcca tcggctcgca cgggcacgag gccgcgtcgt cgctttggcc gtcgacgaca
     2221 tcgtgccacc acacgtctct gccggccgtc atttcgaccg gggttccggt gcggcgcacc
     2281 gtgatgctcg tcgtgaccga cggcgccatc gccatcgcct cgtcggcgat cggcttcaac
     2341 gggctcggct tgccgcgccg ccacgcggcg tcttgcgtca ccaagaactc gcagcgcata
     2401 tcgttcatgc ggctgcccaa cgccgtcgcg ctgaaaccgc cgaacacgaa ggtgaacggc
     2461 gccccgatcc tggccaacgc caaaagcgtc accggcgctt ccggcaccat gcccatgtag
     2521 acggcaacct tggtgccctt gcggacgccc aggctgcgga acgcgttcgc cgccttcacc
     2581 acccggcgct gcaggtcggc gtaggtgatc gccaggcgtt cgtcgaccgg ctccccctcc
     2641 cagaagtagg cgaccttgtc gccccggccc tccgccacat gccggtcgac gcagttgtag
     2701 caggcgttca gccggccgcc caggtaccac ttcgcgtacg ggcgatccca ctgccgaagc
     2761 tgcgtgaacg gcgtgaacca gctgagcctc tcccggccct cccgctccca gaacgcatcg
     2821 ggatcctggt cgtagaagcc aggctgcgca ttcgcctgcg ccgcgaaggc cggatccggc
     2881 cgataccgcc gctcctcgtc cataagcgcg tcgatgttct gatgggcatc cgccatggtt
     2941 gcctctcttc acggggaacg gtgggtgccg gcacgaagga gcaggcccca ggtggtttcc
     3001 gacgagaaag cccgggctcg cggccggcaa ccccattcaa ccgtgcgcag ggtcggcctg
     3061 gcaaaccaac taccgcgcgt agtcgcgaca cccggattca cgccgggcgc ggcagcagct
     3121 cggtcatgtc ggacagtgcc tcggcgagcc ccttgtcgag ggtcgcgagc ctcccctccg
     3181 gcttcgaggc cgccagggag gccaggtggg cgccagtcac ctgccggtgc ccgagcacct
     3241 ggcccagcgc ggggagtcag gccgcgtagt cgccgacgag gcgcacggcg ccgccggcga
     3301 cacccttggc gccctggacg aagccggcga ggtcgcgcgg cccctgccgc acgacaccgg
     3361 cctgccaggt gggaatgccc gatgagacga ggccgcgcct gactccttct gcggcctccg
     3421 gcgcgacgag ggcgaagaag ccgacgccga ggttccaggt gtcttccacg tcggcaagcc
     3481 tcaacccgcc gagatcgcac agcacgcgga agacaggctg cggcgaccag gtcgagcgat
     3541 cgatctcggc ccaggagcct tgcggcagca cccgcgccag gttggccgcc atgccgccgc
     3601 cggtcacgtg ggagagcgcg tgaatggcgc cgccggcggc gtcgagggct gcgagcaggg
     3661 gcgcggtgta cagcctcgtg ggtgtgagaa gctcctctcc gacctcgccc aggccggcga
     3721 ggccgctggt gagggcgacg ccgcggtcgg cgaggatgcg ccgcacgagg gagaatccgt
     3781 tggaatgcag gccggaggag gccagagcga gcacggcgtc gccgccgcgg acccgctcgg
     3841 ggctaaggag cgcgtcggcc tcgacgacgc cggtggcggc gcccgccagg tcgtactcct
     3901 cgggccccat gacgccaggg tgctccgccg tctcgccgcc gacgagggcg gtgccggtct
     3961 cttcgcaggc gcgggcgatg ccagcgacga gggtggcgat gcgcgccggc acgacgcggc
     4021 cgcaggcgat gtagtcggtc aagaagagcg ggcgcgcgcc gacgacgacg atgtcgtcga
     4081 ccaccatgcc cacgaggtct tgcccgacgg tgtcgtgctt gtcgagcgcc tgggcgagcg
     4141 cgagcttcgt gcccaccccg tctgtcgagg tggcgaggag cgggcggcgc atgtcgcgca
     4201 gcgcccgggc gtcgaagagg cccgcgaacc cgccgacgcc gcccacgacc tgggggccgt
     4261 gggtgcgggc gaccgcgtct cgcatgagct cgacggccag atcgccggcc gccgtgtcga
     4321 cgccggctgc ccgatacgat gcgctagcca cccggcaagc ctatcggccg gctcaggaga
     4381 gcactccgtc ggaccagccg acggggatgc cgaggagccg cgtggagccg ggggtggagg
     4441 tgtaggcggt gccgatgacc gcggcggcgt cgatgcctcc cgcggcccgc gcgacggccg
     4501 ccgcccagag cctcgtgtct tctggcttac gggtcacgtc gacggcgatc cacacctggt
     4561 cgggcgtcac ctcacccagc acgtcggcgt actgctccgc gtcgttcggg gccaggccga
     4621 cgccgaccgc gacgagcgtg ccggcacccg tgcgcacccc gtcggcgcgg gcctgcgtgg
     4681 cctcgcggcg cccaccgacg tgacgaaacc ccggccgagc gagggtgccg gccacgccga
     4741 gctgcatgcc cggatgcgcg acggcgagcg cctgggccgc cgcccaggcg tcgtcgcgca
     4801 tgccgaaaag gaagacgagg tcgccggggc cggccgcgag cgtcggtgcc ggcggcaggc
     4861 cgtactccgc gcggtcggac gccagggcac ggccggcggc ggggcgggga ttctccgccc
     4921 gggcctcacg cgccgcactg gccgcggcga cacgttcgcg ctcgtcgagg agctgctcga
     4981 acacctcgcc gaagctgtcg gcgccgcgcc cgctgccggg gagccgctcg tgacgcgggc
     5041 ccgcgaggcc gggggctttc gccgccggtg ccgcctcggg caccgcgacg gcggtggctg
     5101 gggcagtgcc ggcctcgtcg ccccgggcca tcatccgttc gcgttcgtcg gcgtcatcca
     5161 acaggtgaac gaacgactcg tcgggtttga ggtcgtgcac cgccccccgg ttcagcaggc
     5221 tggtcaacga ccatagtcga ggtgtcaacg cggatcactc cgatcgtctc gatggttgcc
     5281 cgggccgcgg tggcctcggt gtaagagagc acggccgtct ccggcgcctg ctgaacgacg
     5341 agccggcgca cggcgggtcg cagctgaggc gcgcagacga ggaccgcctg gtcgtgccgt
     5401 gcggtcgtgg tgcggatggc ggcgatgagt cgttccaacc ggtcgggttc cagcacgatc
     5461 cggctgcccg actcgcgctg cacgaggcct tcgagcagcg actgctccaa gaccggctcg
     5521 aacgtcaaca cccgcaagac gccgtcgtcg aggtaggggg cggcgagcgc ggcaccgagg
     5581 gcggcgcggg cagcctcgat gaggccctcg ggctccgtcg acgccttcgc ccgcaacgtg
     5641 acggcctcgt agatgcggcc gagatcgttg atcgcgaccc gctccgccaa aagcccctgc
     5701 agcacgcgct gcacctccgc caggctgaga agcgcggggg tgagctcgtc gacggcggag
     5761 ggattcaccc gccgcacgcc gtcggtgagc agccgcacgt cttctcggcc gagcaggcgc
     5821 gccgcgttca aggagacgac ctgggagagg tgcgtgacga cgacggagac gcggtcgatg
     5881 accgtcgcac ccgccatctc cgcgttgtgc cgaagctcca cggggatcca cttcgccgcg
     5941 aggccgaaca cgggctcgac cgtctcttcg ccgggaagcg tgccgagccc cgcgccgagg
     6001 gtgagaacct tgccgcgcgg cgcggtgccg cggccggcct ccaccccggc gacgaggatc
     6061 gcgtaggtcg cgggcggcag ctcgctgttg tcgcgggtgc gcaccggcgg cacgacgatg
     6121 ccgaggtcga cggcgagctt gcgccgcagc gcccgcaccc ggccgagcag gtcgtcgctg
     6181 ccgccggaga cgatgtcgat gaggtcggag gccaactgga tctcgagcgg gtgcacgcgc
     6241 atctgggcga tcaggtcgtc ggtggtgtcg gagaccccgg tggccttctc ggcggcagcc
     6301 ttcgatgccg ccgccgcttg ctgcctggct gcgtggaagg cgatgcgccg cgccgcccag
     6361 atgagcagcc cgccgatcgt gaagaacgag agtttcggca tgccgggaat gagggcgagc
     6421 gcgaccgccg cgcacccggc gatcaggagg gccgagcgag actgcgccag ctgcttgccc
     6481 gccgtggagc ccatgtcgga ctcggcgccc gagcgggtga cgatcatgcc ggtggagacg
     6541 gccatgagga gggccgggat ctgtgccgtc agcccgtcgc cgatcgtcag gatcgtgtat
     6601 ttgtgcagcg cgtcggtgat cgagagaccg ccttgcagca tgccgatgcc gataccgccg
     6661 atcacgttga tgacgatgat gaggatgccc gcgatcgcat cccccttcac gaacttggag
     6721 gcgccgtcca tggcaccgta gaagtcggcc tccatcgcga cctcggcgcg acgcctcttc
     6781 gcctcggtgt cggtgatgag gccggcgttc aagtcggcgt cgatggccat ctgcttgccc
     6841 ggcatcgcgt cgagggtgaa ccgggcaccg acctcggcga ctcgctcggc gcccttggtc
     6901 accacgacga attggatgac gacgaggatg aggaacacga cggcgccgat gatgatgttg
     6961 tcgccgaccg cgaccgtgcc gaacgtctcg atcaccttgc cggcgaagcc cgtgcccaag
     7021 acgagccggg tggaggcgac gttgaggccc agccgcagca gggtcgcgat gagcagcacc
     7081 gacggaaaga cggagaagtc gagcggtcgt ttcgtgaaca tcgtcgtcag aaggatcacg
     7141 agcgcgaaga cgatgttgat cgttatgagc acgtcgagca gccacgcggg caccggcacg
     7201 acgaggagca aaacgatgcc gacgacgccg accggcaccg cgacgaggga gaggttctta
     7261 cgtctcatac gctgactccg gtttccttcg acggggttgc ggcggccgtg gcggcgcgca
     7321 ccggggccgg ctgggtcgcc gccccgccct gccggtgacg gcctgtggcc gccggctggg
     7381 ccggcacggt gagcacgtcg ccgaggctcg cgccgcgcct cttcacgagc atgaggaacg
     7441 cgagcacgtt ggcgacgggc acgaagaact cgacggggat ctgcgcgccg acctcgcagg
     7501 cgccatacag ggcacgggcg agcgggatgt cctcgacgat cggtacctgc ttttgtcgcg
     7561 cctcttcccg gatgcgggcg gcgatgaagt cggcgccctt ggcgaccagg cgcggcgcct
     7621 gctgccccgg ctggtaggcg agcgcgaccg ccacgtgggt ggggttcacg atgacgacgt
     7681 tcgcgtgccc gacggccgcg atcatgcggt tccggctcat ggccagctgc atgctgcgtc
     7741 ggtgggagcg cacgagcggg tcgccgtcgg tgttcttcag ctcgtctttg acctcgcgct
     7801 tcgtcatgcg ggtcgaggca cggttgcggc gcaagacgac catcaggtcg agcccggcga
     7861 aggcgagccc ggcgccgacg acgacgagaa gcatcatcgc cgcgctggaa ctgcccgcct
     7921 gcacgaggcc gccgagtggc atccctccgg tctgcagcaa ggtaggcagc cacgcgcgga
     7981 tgacgagggc gaagcccaga cccaccgcga gcgccttcac aagagtcttc gccgcctccc
     8041 acgccgcgcg cgggccgacg atacgcccca gccccttgac gaggttgaac tggcccggca
     8101 tgggcgccag ccgtcgcaga tgcacgccgc cctgcacgac gccggccgcg acggccgtga
     8161 ccccgacggc gagaagcatc gggagcatga tgtggccgag ggtgccgagc ccgtcgtaca
     8221 acgagctgcg cacggcggcg aggctcgggt cggccacgac gtcgccgacg gaggccacct
     8281 gagcgccgag gtcggtgtag gcggaatgca gcgtggtcgg cagcatgacg actccggcgg
     8341 ccaccgacgt ccacgccacg agatcgcgag atttctgcag atgccccttc ttgcgcgcct
     8401 ctttcaggtg tttctcggtc gcctgttcgg tgcgttcctg cgcctctccg gccatcagag
     8461 ccccccgatc agctggcccg ccgaggcggc gagggcgttc acggcggccg gcagcgcgac
     8521 gatgccgaac ccggtcaaga gcacggtgac gagggtgaag gcaggcggcc cgagcgtgaa
     8581 cgcgttcatc gccggcgaga cccgggtgag aaggccgagc gccacgttcg cgaggaacag
     8641 gacgacgatc gtcggccccg cgatctgcag ggcagagacg aagagctgcg tgacggagcc
     8701 ggcaaggctc tgggcgacgc ggtcgatgtc gacgtgcgcg gcgatcggga tcgcggtgaa
     8761 ggaacgcacg aggccgccga ggatcagctg gtatccgtca gaggcgaaga gcagcgccaa
     8821 cgccgtcatc tggtaaaggc gggtgaattg cgcgccgttc gtgccggcgc cgggatcgta
     8881 ttgttcggcg gcggcgaacc cgccgaactg gtcgagaaag ctgccggaga cctgcacggc
     8941 ggcgaaggcg atgtagacga gaaagcccag cgccgccccg gtcacggcgg cgaagacgag
     9001 agcgagaagg aacgggccgg tgtcttgctc cgtatagccg gcggcgaccc tcggcacgag
     9061 aacgagggcc aagagcaccg acagagccgc cttcagctgc agcgggaaag cagtggaaga
     9121 gaacgggggc gcgatggcga gaaaggccgc gatgcgcacc gcgatgagcg cgatcgccga
     9181 cacggtggcc gggtcgaagg tgacgttcat gctcacccgc cgatcagcat ggtgccctgc
     9241 ccgatgagtt cggggatctg gccgatgagg tgctgggtga acgtgacgat ctcgctgatc
     9301 atccagctgc ccgagacgag gagcgcgatc gcgacggcga cggctttcgg aacgaaggag
     9361 agcgtcacct cttgaatctg cgtgaccgac tgcaggagag agacggcaaa gccgacgacg
     9421 agcgcggtca agacgatcgg tgcgcacagt ttggcggtct cgaagagagc ctgcgcggcg
     9481 atgttcataa cggctgcgga atccacgacg ccacctaggt atagctcgag atgagggagc
     9541 ggatgacgag accccagccg tcgacgagca cgaagagcag gatcttgaac gggagcgaga
     9601 tcatgacggg cggcaacatc atcatgccga gagacatgag acccgcggcg acgacgaggt
     9661 cgatgatgag gaacggaatg aagaggacga acccgatgat gaacgccgtg cgcagctcgg
     9721 agagcacgaa cgcgggcacg agggtcaaga acggcacggc cgcagggctg gcggggttcg
     9781 cccggccggc ggcacgggtg atgagggcga tgtcctcctg ccgggtgtgc gcgcccatga
     9841 acgcccgcaa cggcacctcg gcggcgtgga gggcctgggt gaagtcgatg ccgcccgaaa
     9901 gataaggctc gaccgcatgg tggtagatcg ccgcgagggt cggctccatg atgaaaaggc
     9961 tgagaaagag cgcgagaccc gccaacactt ggttcggcgg aatcgtctgg gtgccgagag
    10021 cgttgcgcgc catcgcgagc acgacgaaga ccttcgtgaa cgaggtcatc atgatgagca
    10081 gtgccggcgc caccgagagc agcgtgatgc cgaggagggt gacgatcgcg tcggccggct
    10141 tgccgttcgg tccgttgatg gagatcgtcg cggccgcgac ctgtgccgcg acgatcaatg
    10201 acggctcttt ctcgtcttgg caagtggtcg ccactgcgag cgcgagaaga ctgcggaaga
    10261 ctcggcgcgg ccgagcatgc gggcgaagtc gtagaccggg tgggagtctt cggcccgcac
    10321 gtgccgcgcc gcgtcggccg agcggccagc gacgcccgcg gctgtcgcgg cctcatcgtc
    10381 ggccccgccg gccacccgca gcggcggcac ggcacgcggc gcgaggggcg cctcccccgc
    10441 ctccgcctgg gccgggaccc gtgaggagtg cagcacgttc acgctgtgct ccgtcacgcc
    10501 gagcgtgaac cgggtgccgt ccacgtcgac gacgacgacg ctcgccttcg agccgagcga
    10561 gcgcttgccg gcgacctcga tcgtcgttcc cgagtggtgt ttggccgccc accgcaggga
    10621 ccggcgttgc agcacccaga gaagggcgac gacggccccg agtgcgacaa gcacccgcag
    10681 agccaagagg agagtgtcca cgctcaggcg acgtcttcgg cggggtcgag aatctgggtg
    10741 acgcgcaccg cgtagtcctg gtcgacgacg acgatctcac cgtgcgcgat gagcctgccg
    10801 ttcaagagga tgtctgccgg ggagcccgcg gaacggtcca gttcgatgac tgcgcccggt
    10861 tcgatggcca agacgtcacg caccgccatg cgggtgtagc cgatgcgcac ggtgagcgcc
    10921 atctccacgt tcgagatgcg ggagagcctg ccggtcacgt cggccgccgc ggcgcgcgcg
    10981 ggtgccgacg cgcgcaaggt gcgtgccgcg aaccaggcga tctcgccgcc cgattcgtcg
    11041 acgacggcga aggagtcggt ctcggggtcg gcaaagacgt ctcgcgcgtc gacgacgctc
    11101 gcgtcggaga gcgtgcccgg cccgagctcg gcggccgcgg tcgcgaggag cggccggagc
    11161 aggtcggcgg gcccgagccg gtcgctcgcg ccgcctaggt cgtagccatc cgccagcgcg
    11221 acgacgagct cggaggacga ggcgcccacg aaccgggcgg tgacggcgtg ctccgccgcc
    11281 gacggcaccg gggcgccggc gggccgggcg gccacggtga gggggccgga gaacagcggc
    11341 gcgtgggcga gggtctgggc cgccttctcg tatgcgttca gggtcatgct ccttcttcca
    11401 ggtcggtcac gacgacggcg agccgcgaac ggctggtgcc gatcgcgacg gaggcgacac
    11461 gctgctcgcc ggtcacgagg ttcaacgggt gatgggtggg gtgcggcagg cgcaacacgt
    11521 cgccgacctt caggctcagc acctcggagg gggtcacgta ggccgggtcg aggctggcgg
    11581 cgacctcgat cggcacctgc acgagctggc gacgcaggta gtgggtcgca tcgtcgctcg
    11641 gcacggtcgg gttcgcctcg cccagctgcg gcaggagcac ctgccacggc agggcgaggc
    11701 tcgcctccgc ctcgttgtcg ccgacgcgca gggtgaaacg ggcgatgacc atcagctcgg
    11761 tggtcggggc ggcctgcgcg aactgtgaat gatattggat gctgtccacg cggatgctgc
    11821 ccggcaagag cgggccgagc gaatatttga ggtcttcgag cgcgtcgtcg gtgatgtgtc
    11881 gcacgagcgc gtactcgatc ggggtgaagg tgcgctccac cggttgcacg ggccgggtgc
    11941 cgcccagcat cgagttgatc caggtcaggg cggagggcgt ggggaattgc aagacggcac
    12001 gcggctcgac gtcgctcagc ccgagaagga tcatggcggt cgtcgcgggc agcccggagg
    12061 cgtactcgtc gtagcccagc atccgaacgc tttggaacgt gacctgggag agcacgcgga
    12121 tcttcgccgt cagctgggtg ccccactggc gggcgaacgt gtcgaaggcg agttcgaggg
    12181 tgcgagaatg ctcccgcacc agggtcgtcg gccggcgaaa gtcatacagc cgggcctcgc
    12241 cggcccggtc catcgcgacg gcgctcatgc ggcgggcact tcttgcccga ggcccccggc
    12301 gggcgcgcct tgcgccgtgc cggccggcgg tgcggcgggc ccggcaccct gctcctcccg
    12361 gagaagcttc gggatgagca tgcgtacgtc ttcggactgg ccagagagga tcaccatgtc
    12421 gactcgacgc tcgtcggcca tgtcttgcgc gctgtcgccc ccctccttcg cctgcacgtc
    12481 gccgtagctg accgcgtcga gccgggaggc gggcacgccg tcaccgttca aggagcgcaa
    12541 gacggcgata gaacgggccg cggccagctc ccagttcgtc gggtaaggcg gcagtggagg
    12601 gcggccgtcg gcatgcccct cgagcgagac cttgttgtcg accgtgtcga tgatcggtgc
    12661 gatcgccgca ataacccgtt ccgccgtggg ggtcaggtcg gcgaggttcg tgtgaaagaa
    12721 agtcttgttg ccgatcaagc cgatcgtgag gccgcgctgg tcgatggtgt acgagacgtc
    12781 tttttcgaga cccttctcgg tgaggctctt gttgatcgct gccttcaacg cctccagctt
    12841 gtcgagttcg accttcgcga gggccaccgg agaggggtgg gtcgccttcg ggtcgttcac
    12901 gtctttcggc ggcacgacca cgccgtgggc cgtgtcgacc ttctgcgtct tgacgatgcc
    12961 gaacccggtc tgcaaagagt tcttcagttc tttgaacttg ttggcgttga cgctcgacat
    13021 cgcgaacatg acgaggaacg tgcacatcaa gacggtcacc atgtcgaggt acgaggccat
    13081 ccaccgctct tcgccggcgc cgtgcgccgc ctcgtcgtcg gtgctcgcac cgcgccgggc
    13141 gcggccgggg cggtgccggg tcacgcgttc accgattcct tgcgctcggc cttcgtgccc
    13201 cggcccttgg ctttcgcccc gcgctccggc gcctcgccgc ccttgccgtc tttgtctttc
    13261 ttctccttgt cgccgccttt gccggtctcg gccggcacca gaacggccag gcgctcggcg
    13321 agcgcccgcg aggagacgcc ggcttggatc gcgagcacgc cctcgagcac gaggttctgc
    13381 cgctccacct cgaggtcgca cagccggccg atgcggctgc cgatcggcag ccagatgaag
    13441 ttcgcggaga gcagccccca gagggtggcg atgaaggcgc tcgcgatcat cgggcccagc
    13501 gcgttcgggg aagaaaggtg ggccagaacg tgggtgagcg agacgacggt gccgatgatg
    13561 ccgacggtcg gcgcgtaccc gcccaagacg ccgaaaaact tcgcaccccg gcgaaggccg
    13621 gccgcccgcg tgtcgatctc gtcttgcagg cgggtggcga ggtcgtcgga gtcggcgccg
    13681 tcggcgacgc cctgcatcgc ggtcttcagg aacgggtcgt cgatctcctc cgccgcgctg
    13741 tcgagagcga gcagcccgtc gtggcgcacc aggtcggcca gccgcacgag ggcggcgatc
    13801 gtgcctgcca tatcaggccg cctgccgcgg aaggcggcgg gaacgagctt gaaggcggct
    13861 atcgtgtcgt gcatcgtggc cccggcgatg ccgacgccga tcgtgccgcc gaagaccagg
    13921 atgagcggcc cgggcagaag cagggaactc gggtcggaac cctcgatgaa gatcgtggcc
    13981 gcgagcgagc cgaaggcggc gagaaggccg acgatggtgg cgagatccac gatcactcgc
    14041 ctccgatcat cgtcaggagc gagtcgccgc cgccgtcttt cgacgaggcg accgccagca
    14101 cccgggcgcg gtagtcgacg atgcggtcga tgacggtctc gaggctctcc cgcacgacgt
    14161 agcagacgcc gtcgaccatc gtgatggtgg aatccggcga cgcggtgatc cgttcgatga
    14221 ggtcggggtt cacggcgagc cggccaccgt tcagcctggt cacgaggatc atgcggcagc
    14281 tgctcctggt tgtcggtgcg ccgcgacggc cgcggccttg atcgtcagtt ggtcttcagg
    14341 ttgacgagtt cttgcaagac gtcgtcagag gtggtgatga cgcgggcgga ggcctggaat
    14401 gcacgctggg cgacgatgag gttcgtgaac tcacgcccca ggtcgacgtt ggagccctcc
    14461 accgcgtcgg cgctgatcgt gccgctgccg ccgctgcccg gcgcgccgac ctgtgcgagg
    14521 ccggagttgg ctgtggagga gaagagcgag tcgccgagct tctctaagcc ggaagggttg
    14581 gagaacgtcg caatcgcgac ctggccgatc gcctgtttcg cgccgttcga gaacgcgcct
    14641 tcgatggtgc cgtcggtgcc gatcgtgaac gactgcaacg ttccggccgc gttgccgttc
    14701 tggtcggcga cattgaccga ggtcgtgccg gcgtatccgg tcagcttcga catgtcgacg
    14761 gtgatgccgt cgctcaagct catcgtgccg ccggaggtca ggctgccgtc gctgccgaac
    14821 acgacggtgc cggtgccgag gctcgcgccg gtctcgtcgg tcgccgcgac attccaggtg
    14881 gcgttgttcg acccgtcgac gccgccgaag gtgtacgtga gggtgatgct gtgcggcttg
    14941 ccggcggagt cgtagacctg agcctggctc gtgagggtcg tgggggtggg cgtcgtcgtc
    15001 gcgtcctccg gcagattgcc gctcatcgtg atgctcgtcg tcgcggtcgc cggcaccatg
    15061 ctgccgagcg ggatgtgtag cgtgccgggc gcggcgccac gctgaacctg gccggttccg
    15121 ttcgccgacc agccctgcac aagggagccg tcgggcgcga cgaggtcgcc ggcgccgtcg
    15181 agagtgaacg cgccggcccg cgtgtaggcg gtctggccgc cgttgttcac gatgaagaag
    15241 ccgttgccgt tgatcatcat gttcgtgcct tgcccggtgg tctgggtggc gccgccggtg
    15301 aagtcggtgg cgatgcccgc cacctcgacg ccgaggccga cctgcgcggg gttcgtgccg
    15361 ccgttgttcg cgttcggccc ggaggcgttg tgcagcactt gggagagcgt gtcttcgaac
    15421 tccaccgagg tggccttata gccggtggtg ttgacgttgg cgatgttgtt ggcgaccacg
    15481 tcaagcatgg tctgctgcgc ctggaggccg gagacaccgg agtcgagaga acggagcatg
    15541 aggtgccttt ctgagctgtc cggttcgaag tcgtgggctg tgcgtctgat gtggtgtggg
    15601 gatgcgtggc ccgccgccgc gccttagggg cgggcgctca ggcggtggat ccggccgcag
    15661 cggcgccgga ggtgacgccc gccaccgaag acagcggcaa cgagacgccg ccgatcgtca
    15721 ccgtcggcgt ggtggtaccg aacgtgaccc cttgcacgac tcccgtcgcg ctggcgccgt
    15781 tcgcgtccgt gtactgcacg gtcagcccga caagggaggc cgccgtctgg cgcatctgaa
    15841 aggtgagggc ctggttggcg tcgctctcca tctgagtgag ctgctgcagc atgccgagct
    15901 gcgtggtctg cgccaccatc tgggtggcgt ccatcgggga gctcgggtct tgattctgca
    15961 actgcgcgag gaggaggctc atgtaatcga tgctctggcc ttgcgcgtcg tcggcggccg
    16021 gggcaggtgg cgccccggca ctcgtgacgg gcaccgagga tcccgccgtg gcgggtgtcg
    16081 gtgcgcccag tggagcgagt gctgacatct ttctccttcg tcccgtccaa accgtctggc
    16141 cggtcaggcg aaaatatcga gcaccgacgt gctgccgggt aaggggcgcc tgaccgggtg
    16201 ctcggaatcc tgcctccgct cgtctgtgag gcgagcgccg tcctgtggcg ggccccaggt
    16261 cgccgtttga ccctgacctc ctgcagcctg ccctccacta tcggcaccgg atccgctcgg
    16321 gtcagaaagc gtgagaaacc cgccggagac ggtctggccg aggtcgtgat gcagctgcgg
    16381 caagagctgt cgcagggcat tctgcccggc gtcggtggag gagaagagct gcacgtgcac
    16441 gccttgctcg gtcacgtcga cggtgaccgt gacggtaccc agattctcgg gggtgacccg
    16501 cagctgcagc gtgtgcatcc cgctcccctg cgccgcgagg gccgcaatct gcggcacgag
    16561 ctgcggcagg aggccgccgg ccaggggaga aagcgccgcc tgggctggtg tgggtgccac
    16621 accgacaagc gcgttttggg aggaaggtgc tgccgcgccc agggcctggg cggcggccga
    16681 tgcgggcgcc ggagccgggc ccgatgcgtc gcccgacgcg ctcttcgaag aaccgccgtc
    16741 accgggcccg ggtgagggcc tgacggccgt ggccggggcc tggctggcga gggcctgggc
    16801 gcccgagacg ctcgcccgcg gcgcagccgt gccggtttga gcggccgccc ggccggcgag
    16861 gcggcccggc ccttgcggct gcggggctcc gggcaggccg cggccgctct gctccatacc
    16921 tccgcctgca cccgcacggc cggccggcat ggcgacgccc gcctgcgaca cggcacccga
    16981 cagggcggcg ctcgccgtgg ccgtcgtcgc tcgtgaggcg gacgccgtgc ctgcgccggc
    17041 ggcgcccgac gccaagaccc ccgcgtaccc gccgcccgcg acggcggcac gcggcgtgcc
    17101 ggcgacaccc gcctgtggca ggagcgtgga cgccccagca atcgccgtgc ccgtgacggg
    17161 cgacgacggc acgccggccc ccggcccggc cttgccgccg accgccgagt cgaccgccga
    17221 ggcctcgccc accgtgccct gcactgcggc gaacgggccc gcggcgacgc ccgataggcc
    17281 gggtacggca cggcccccgg ccgaaacccc ggcggcagcg gacccgccgg ccccaagcat
    17341 cgtcgcggct agcgtcgcga cgccaggccc gggcgccgcc acgtgcgccg cggccgcaga
    17401 agacgccgtc atgccctgcg cggcgagaga cgcggtgccc ggcgctccgg cagggccggc
    17461 cggcgctgcc gcggcggccg ccaacgcagc ctgcacggcc gggccgaccc gcgcggccga
    17521 agcatcctgg gcgaccgagc cggctcgcgc tgccgaacca gcctgtgtgg tcgggccggg
    17581 ctgcgtcgcc gggcccgcct gcgccgatcc ggcctgcgcc ggggccgcgg ccgagagcgc
    17641 tgcgaagcca agggcggccg tgcaggcggc cccgggaccg ccgtcatcct tctcgtgttg
    17701 gctgcttgaa cgggcccgcg cgctactcgc gccggggcct gcaaggtcgc gcccgcccgc
    17761 ggcgaggcgc gctccccgcc ctgggtgctc cggccctggg ccgttcggcc gtactgctgc
    17821 ggatgcgggc cgcgcctgcc ccgcgcccgg gtcggcggcc gcgtgcagaa tctgggcgaa
    17881 gagatcccca cccgtgccgc ccggctgcgc ggccggccgc g
//
LOCUS       DASTNL010000041         6552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_402174, whole genome shotgun sequence.
ACCESSION   DASTNL010000041 DASTNL010000000
VERSION     DASTNL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402174"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..952
                     /locus_tag="VFQ96_01205"
     CDS             227..952
                     /locus_tag="VFQ96_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0256437.1"
                     /translation="MMVHAAKNGQAGGATATRRPPVVRHYGDGVDRVFEEYGKAGDPD
                     VVLLHGGFWRADTNRGYLRPMSRQLAAAGTHVYLPEYRRTAGSPMHSLDDVEDFLGFL
                     AGRGRLVRVMVGHSAGGHLALLHAAAYKTNSPQVVALAPVADLFVHAKDAANGEADIR
                     SWIGASAQERPALWHRLNPAEHLGRRPSRVTVLHGDADEVVPLSVTAGIERTVLPGAG
                     HEDLIDPESRHFRAVLEAVATRL"
     gene            982..1581
                     /locus_tag="VFQ96_01210"
     CDS             982..1581
                     /locus_tag="VFQ96_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEU0256438.1"
                     /translation="MGVGAALALALVGLGAAGPAAAASAEGGLQPVSWSPGPSSVVTT
                     TLSQVQVTFNEPIKLPSQGVALWVSGPNYESQYFETACATLRGRTLSVPVAMGTPGSY
                     RVSYNVVAPNGKLIFNTMNFDYDPPRGTPEAAGNDSLVCSGNHAASADTSNAPGTQAV
                     PLSRGTLAGLGLLAALCVAALVFAGTRLFGGEPKSRPKG"
     gene            complement(1609..4077)
                     /locus_tag="VFQ96_01215"
     CDS             complement(1609..4077)
                     /locus_tag="VFQ96_01215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU0256439.1"
                     /translation="MTQTPPRHGRARQGRFARPRRRRRRIAVIASVTAASLLAVLIVG
                     VGLGAASLALLPGYLKIPTLQQASTIYANENGKPVPIATFFAQNRVLATWDEVSAKVK
                     DAAVDTEDPNFYKEGGISIRGTLRGAIQTFLRHDTQGGSTITQQYVKNVRTQLCNEEY
                     MAPPDASEKVAQKVREEWQACNRDASGVTVTRKLQEIRYATALEKRYSKKQILLGYLN
                     IAFFGGNVYGIQAASHYYFDTTAKNLTLPEAATLVAIVNSPNNLRLDEDAKANPGNNA
                     GNGFAAAKDRRDYVLGKMLKAKDITQAQYQAAVETKVSPKITPVHSGCMSADKYDAGF
                     FCNYVQHEILTDPAFGANPEDRLATFQRGGMKIYSTLDLNLQKTAHDALGAYIPPTRQ
                     GMELGGTQVAMRVGTGQIVTMVENRRYNDTNSPPAGSTSINYATDFNQGGSAGFQTGS
                     TFKAFVLAAWLEAGNTLGQIVNANNHYFPASDFHSTCGDSAWANWPVQNSEASEGGYM
                     SVLNATANSVNTAYAEMGTQLDLCSISKIAESLLVHSASPSTNPWQVLPPMILGSNYI
                     SPLSMATAYAAFANKGVVCTPVAISSVVGPGGKKMQAPKSQCSQAIPENIANTVAYAL
                     QSVMRYGTAVAANPNDGIPILSKTGTTTNAVDNWLVSSTTKVASALWIGNVKGNTNLR
                     TVYFSPTSGYGPVYSGMNVKFAVDKPILQALNARFGGAAFGAPDQALVYGSQGGPPSS
                     APATTSAPATTSAPADTATPPPTVSTPPAATSPVTPPPGQVTSPADPGGEGGSQGGAG
                     GSAGQKSPDGQGPQPTAGDLRPGG"
     gene            complement(4142..5050)
                     /locus_tag="VFQ96_01220"
     CDS             complement(4142..5050)
                     /locus_tag="VFQ96_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256440.1"
                     /translation="MAIPFGLSGLAVAWTLAQKDLGAWAWMGVLAWALAAAAWLYFSA
                     AWLRGALTAHRVLADLRHPLLGPFVALVPVVAILLGGQSAHYVPAVGAGLVVAALVVI
                     GIMSAWSLSAILAGTVDAHVFHSGYFLFILAGGLIGAIGLAEIGALRAATAAFAIGVF
                     FWAVLAAVVVARLAAGPAIADAYVPTLAIFSVPPSVAGLGWFGIHGSQIDWVQDTLLA
                     LTVFLVLSQGFLVHRYAKLPFSLGFWSFTFTAAAPVSYGMHWLAATTPPGWRIWSWVM
                     LLAVTLWIVWIAVRSVPLLFGRGARR"
     gene            complement(5079..5792)
                     /locus_tag="VFQ96_01225"
     CDS             complement(5079..5792)
                     /locus_tag="VFQ96_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU0256441.1"
                     /translation="MPDIDLFPPATGTHIVLLVVAFVLSAIVGLEQQRRLKSAGLRTH
                     TLVGVGAALFTLVSAYGFAPVLGPGVVLDPSRIAAQIASGIGFLGGGVIFVRQNVVNG
                     LTTAASIWVTAAIGMACGADMPVVAAAGTVLYLVTIIGLSPLGRAIRVRQWGKEEQCI
                     IHYKDGKGALRVVLEKASILGFEATLLDTRIVEKSGKRDRVEARFRFSGRTPLESLVE
                     ELSEIDGVTKVALSHADDD"
     gene            complement(5779..>6552)
                     /locus_tag="VFQ96_01230"
     CDS             complement(5779..>6552)
                     /locus_tag="VFQ96_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256442.1"
                     /translation="RSVSLEFIDCPDRRVTRAAVDLVAREAEAPGTQVTAILPRRSFS
                     PLLGRLLHDRTADRLARVISHIPQAAATIVPYDVQAHIRSAMPNLPEERITRSIERLA
                     SRLTGHDDEDLATATTAAPHAGPGSSPIGVLVPGRKAVVEAYLTELSTDGRREHGHTV
                     LACSLADDTARLSVTFSRAADGLEVGQRLRLTGVPHRDGPGRPLVMADPEFHVVLDPD
                     AADDEADAEDAGTVPSSDARNRDAAGDEKAGTGEDDARH"
BASE COUNT         1012 a         2226 c         2286 g         1028 t
ORIGIN      
        1 ccggtcggcc gcgagcacgc cggagaatcc gtgcgcctcg gcgtacgccg acaaccggac
       61 cgcctccgcc ggcgtgaacc gttcgagcat ggcggcgtag ccgatcctcc cctgccccga
      121 caccgtatca atctatcgcc cataggccag gccggccctc cccggcaccc gcccgccgtc
      181 ccatcgaaca cccatccccg cggcgtaggc tcgccgcgag ggaggcatga tggtccacgc
      241 agccaagaac ggccaggcgg gcggggcgac ggcgacgagg cgccccccgg tcgtgcgcca
      301 ctacggtgac ggggtcgacc gcgtgttcga ggagtacggc aaggctggcg accccgacgt
      361 ggtgctgctg cacggcggct tctggcgggc cgacacgaac cgcggctatc tgcgccccat
      421 gagccggcag ctcgcggcgg caggcaccca cgtctacctt cccgaatacc ggcgcaccgc
      481 cggcagccct atgcactccc tcgacgacgt ggaagacttt ctcgggttcc tcgccggtcg
      541 cggccggctc gtgcgtgtca tggtcggcca ctcggccggc ggccacctgg cgttgctgca
      601 cgccgccgcc tacaagacga acagcccgca ggtggtcgcc ctcgcgcccg tcgccgacct
      661 gttcgtgcac gcgaaagacg cggcgaacgg cgaggccgac atccgctcct ggatcggcgc
      721 ctcggcccaa gagcggcctg ccttatggca tcggctgaac ccggccgaac acctcggccg
      781 gcggcctagc cgcgtcacgg tcttgcacgg cgacgcagac gaggtcgtgc cgctgagcgt
      841 caccgccggc atcgagcgca cggttctgcc gggcgccggg cacgaagacc tgatcgaccc
      901 cgagtcacgt cactttcggg cggtgctgga ggccgtcgcg acacggttat gatggcgcgc
      961 cgacccgcta tcgcagctcg cgtgggcgtc ggggcggccc tcgccctcgc gcttgtcggc
     1021 ctgggcgcgg cgggtcctgc cgcagccgca tcggccgagg gcggcctgca gcccgtctcc
     1081 tggtcacccg ggccctcctc cgtcgtgacg acgaccctgt ctcaggtcca ggtgaccttc
     1141 aacgaaccga tcaagctgcc gtcgcaaggc gtggcgttat gggtctctgg gccgaactac
     1201 gagagccagt acttcgagac ggcctgcgcc accttgcggg ggcgaacgct gagcgtgccg
     1261 gtcgcgatgg gcacacccgg cagctaccgc gtctcctata acgttgtcgc gccgaacggc
     1321 aagctcatct tcaacacgat gaacttcgac tacgacccgc cacgcggcac ccccgaggcg
     1381 gccggaaacg attctctcgt ctgctcgggc aaccatgcgg cctcggccga cacgtcgaac
     1441 gcgccgggga cccaggcggt tcctctctcc cgcggcacgc tggcgggcct cgggctgctg
     1501 gccgcgctgt gtgtcgcagc gctcgtcttc gccgggacac ggcttttcgg cggcgagccg
     1561 aagagccggc cgaagggctg agaaccccgc cgaggcgggg cctcttgcct agccgccagg
     1621 cctcaggtcg ccggccgtgg gctgaggccc ctggccgtca gggctcttct gaccggccga
     1681 ccccccggcg ccgccctggg aaccgccctc gcctccgggg tcggcggggc tggtcacctg
     1741 accgggtggc ggcgtcaccg gcgaggtcgc ggccggcggc gtgctcacgg tcggcggcgg
     1801 cgtggcggtg tcggcgggag ccgacgtcgt ggccggcgcc gacgtcgtgg ccggggcgct
     1861 cgacggcggg ccgccctgcg aaccgtatac caacgcctgg tcgggggcgc cgaacgccgc
     1921 tcccccgaac ctggcgttca aggcctgcaa gatgggcttg tcgacggcga acttcacgtt
     1981 catgcccgaa tagaccggcc cgtagccgct cgtcggcgag aagtagacgg tgcgcaggtt
     2041 ggtgttgccc ttcacgttgc cgatccagag cgcactcgcc accttcgtcg tcgaagagac
     2101 gagccagttg tctaccgcgt tcgtcgtcgt gccggtcttc gacaaaatgg ggatgccgtc
     2161 gttcgggttg gcggcgacgg ccgtgccgta acgcatcacg ctttgcaacg cataggcgac
     2221 cgtgttggcg atgttctcgg gaatcgcctg gctgcactgc gatttcggcg cctgcatctt
     2281 tttgccgccc gggccgacca cggaagaaat cgcgaccggg gtacagacga cgcccttgtt
     2341 ggcgaaggcc gcgtaagcgg tcgccatcga caacggcgag atgtagttgc tgccgagaat
     2401 catgggcggc agcacctgcc acgggttggt gctgggtgag gcagaatgca cgagcaacga
     2461 ctcggcaatc ttcgagatgg agcacaggtc gagctgggtg cccatctcgg catacgccgt
     2521 gttgaccgag tttgcggtcg cgttcaagac cgacatgtac ccgccctcgg aggcttccga
     2581 gttctgcacg ggccagttcg cccacgcgga atcgccgcac gtggagtgga agtcagaggc
     2641 cggaaagtag tggttgttcg cattgacgat ctggccgagc gtgttcccgg cctcgagcca
     2701 cgcggcgagt acgaaggcct tgaacgtcga cccggtctgg aagccagccg agccgccctg
     2761 gttgaagtcg gtggcgtagt tgatggaggt cgagccggcg ggcgggctgt tggtgtcgtt
     2821 gtatcgtcgg ttctcgacca tggtgacgat ctggccggtg ccgacccgca tagccacctg
     2881 cgtgccaccc aattccatgc cctgcctcgt gggcgggata tacgcgccaa gcgcgtcgtg
     2941 cgcggtcttc tgcaggttga ggtcgagggt gctgtagatc ttcattcccc cgcgctggaa
     3001 cgtcgcgagc cggtcctccg ggttcgcacc gaaggccggg tcggtcaaga tctcgtgctg
     3061 cacgtagttg cagaagaatc ccgcgtcgta cttgtcggcg ctcatgcagc cgctgtgcac
     3121 cggcgtgatc ttcgggctga ccttcgtctc caccgccgcc tgatactggg cctgtgtgat
     3181 gtccttcgcc ttcagcatct tgccgagcac gtaatcccgt cgatccttgg cggcggcgaa
     3241 cccgttgccg gcgttgttgc cggggttcgc cttggcgtcc tcgtcgaggc ggaggttgtt
     3301 ggggctgttg acgatagcga cgagagtggc cgcctcgggc agggtcaggt tcttggccgt
     3361 ggtatcgaag tagtagtgcg aggcagcctg gatgccgtag acgttgccgc cgaagaaggc
     3421 gatgttgagg tagccgagca atatctgctt cttcgagtag cgcttctcca gcgcggtcgc
     3481 gtagcggatc tcctgcagct tgcgggtcac cgtcaccccc gaggcgtccc ggttgcacgc
     3541 ctgccactct tcgcgcacct tctgtgcgac cttttccgac gcgtcgggcg gcgccatgta
     3601 ctcctcgtta cagagttggg tgcgcacgtt cttcacgtac tgctgggtta tcgtcgaccc
     3661 gccttgcgtg tcgtggcgca agaaggtctg aatcgcgccg cggagggtgc cgcggatgct
     3721 gatgccgccc tctttgtaga agttcgggtc ttcggtgtcg acggccgcgt ctttcacctt
     3781 cgccgagacc tcgtcccacg tggcgagcac ccggttctgc gcgaagaacg tcgcgatggg
     3841 caccggcttg ccgttctcgt tggcgtagat ggtgctcgcc tgttggagag tcgggatctt
     3901 caggtagccc ggcaggagcg cgagggaggc ggcgccgagg ccgaccccga cgatgagcac
     3961 ggcgagaagg ctcgcggcgg tcaccgaggc gatgacggcg atccgacggc gacggcggcg
     4021 aggcctcgcg aaccggcctt gccgggcgcg tccgtggcgc gggggtgtct gtgtcatgcg
     4081 ttttcttccg caggcagcgt accggcccct tcctgggtga gcgctggaac cggagccact
     4141 ctcaccggcg cgcgccgcgt ccaaagagaa gaggcacgga gcgcactgcg atccatacga
     4201 tccagagcgt gacggcgagg agcatcaccc acgaccagat ccgccaaccc ggcggggtgg
     4261 tcgcggcaag ccagtgcatc ccgtacgaga ccggcgcggc cgccgtgaag gtgaaagacc
     4321 agaaccccag cgagaacggc agcttggcgt agcggtgcac gaggaaaccc tggctgagaa
     4381 cgaggaacac ggtcaaggcg agcagagtgt cttgcaccca gtcgatttgc gagccgtgga
     4441 tgccgaacca gccgagcccg gcgacgctcg gcggcaccga gaagatggcc agcgtcggca
     4501 cgtaggcgtc ggctatcgcc gggcccgccg ccaggcgcgc caccacaacg gccgcgagca
     4561 cggcccagaa gaacacgccg atcgcgaagg ccgccgtcgc ggcccgcagc gcgccgatct
     4621 ccgccaaccc gatggcaccg atcaaaccgc cggccaggat gaagaggaaa taccccgagt
     4681 ggaagacgtg cgcgtccacg gtgcccgcga ggatggccga cagcgaccac gcgctcatga
     4741 tgccgatgac gacgagggcc gcgacgacga gaccggcgcc cacggccggc acgtagtggg
     4801 cgctttgccc gcccagcagg atcgcgacga ccggcacgag ggcgacgaac ggcccgagga
     4861 gcgggtgacg cagatcggcc agcacccggt gagcggtgag tgcgccccgc agccacgcgg
     4921 ccgagaagta cagccaggcg gcggcggcga gagcccacgc gagaacaccc atccatgccc
     4981 aggccccgag atccttctgt gcgagagtcc aggcgacggc cagcccgctc aagccgaagg
     5041 ggatcgccag ccggttcggt gcggcccttc tgcccgtttc agtcgtcgtc ggcgtgagac
     5101 aacgccacct tcgtcactcc gtcgatctcg gaaagctcct cgaccagact ctcgagcggc
     5161 gtgcggccgc tgaagcggaa ccgtgcctcg acgcggtcac gcttgcccga cttctcgacg
     5221 atgcgcgtgt cgaggagggt cgcctcaaag ccgaggatgc tcgctttctc cagcacgacg
     5281 cgcaacgccc ccttgccgtc tttgtagtgg atgatgcact gttcttcctt tccccactgg
     5341 cgcacgcgga tcgcgcggcc gagcggcgac aacccgatga tcgtcacgag gtagagcacc
     5401 gtgccggccg ccgcgacaac cggcatgtcc gcgccacagg ccatgccgat ggcggcggtc
     5461 acccagatgg aagccgcggt cgtcagcccg ttgacgacat tctgcctgac gaagatgacc
     5521 ccgccgccca ggaagccgat gccggacgcg atctgggccg cgatgcggga ggggtcgagc
     5581 acgacgccgg gccccaggac cggggcgaat ccgtaggcgg agacgagagt gaagagggcc
     5641 gccccgactc cgacgagcgt gtgggtgcga agccccgcgc ttttcaggcg acgttgttgt
     5701 tcgagcccca cgatcgccga gagcacgaac gccacgacga gcaagacgat atgcgtgccc
     5761 gtcgcgggtg ggaaaaggtc aatgtcgggc atcgtcctcg cccgtgcccg ccttctcgtc
     5821 accagccgcg tctcgattcc gagcgtcgga ggacggcacc gtgccggcgt cttcggcgtc
     5881 ggcctcgtcg tcggccgcgt cggggtcgag gacgacatgg aactccggat cggccatcac
     5941 gagcggccgg cccgggccgt cgcgatgggg cacgccggtc agccgcaacc gctggccgac
     6001 ctcgaggccg tcggcggcgc gcgagaacgt gaccgagaga cgggccgtgt catcggcgag
     6061 gctgcacgcc aagacagtgt gcccgtgctc gcgccggccg tcggtggaaa gctccgtgag
     6121 gtaggcctcg acgaccgcct tgcgtccggg cacgaggact ccgatcggcg agctgccggg
     6181 ccccgcgtgc ggcgcagccg tcgtcgcggt cgccaggtct tcgtcgtcgt ggcccgtcag
     6241 gcggctggcg agacgctcga tcgaccgggt gatgcgctcc tccggcaggt ttggcatcgc
     6301 cgaacggatg tgtgcctgca cgtcgtaggg cacgatcgtg gcggccgcct gtgggatatg
     6361 gctgatgacg cgcgccagcc gatccgccgt gcgatcgtgc agcagccggc cgagcagcgg
     6421 tgagaagctg cgccggggca agatcgcggt cacctgggtg ccgggcgctt cggcctcacg
     6481 ggcgaccagg tcgacggcgg cccgggtcac cctgcggtcg gggcagtcga tgaactccag
     6541 cgacacgctg cg
//
LOCUS       DASTNL010000042         8358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_958302, whole genome shotgun sequence.
ACCESSION   DASTNL010000042 DASTNL010000000
VERSION     DASTNL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958302"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFQ96_01235"
     CDS             complement(<1..756)
                     /locus_tag="VFQ96_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0256443.1"
                     /translation="MGAGASRGGDADAIVVGSGPNGLAAAVTLARAGLRVVVVERSDH
                     VGGGAATRELLVPGVWHDVCSAVHPLALAGEFFRRFHITERVRFLTPEVSYAQAVTPA
                     HAALAWRSLDRTADALGEDGDAWRRLLGPLAAEPARLWNLAGRPAWRVPDDPAFLLRF
                     AARVARHGIRGRAGGFRGDDAPALLAGVFAHTTGPLPGLGAAAAGLVLTGLAHAGGWP
                     IPYGGSASIVRALTADLAAHGGLIETGVEVTDLG"
     gene            821..1105
                     /locus_tag="VFQ96_01240"
     CDS             821..1105
                     /locus_tag="VFQ96_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256444.1"
                     /translation="MDPATRRLMFWLKAPYLVDVALGLVGAALLAAGRAAGWWVILFG
                     AGRAALATAALIWLTPRLRRRSAPGSGAGAARQVTSRRGRRPWRPSRRRT"
     gene            complement(1053..3758)
                     /locus_tag="VFQ96_01245"
     CDS             complement(1053..3758)
                     /locus_tag="VFQ96_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU0256445.1"
                     /translation="MSDDKAARTTGRSADGNARRAWYRRVDSLVLAWALAAAVAGVIP
                     WRGQATAWLSVHLVLLGAATAAIMIWSQYFADTLIHRAGDGRRGLAARLALHTPGTLA
                     VIVGVPFGLWPLVVAGAAAVAVAIVWHVAALYLRVRSTTTRFSIVLRYYVVAGLMLVV
                     GICLGVVMAGADAGQAAHGRLLLAHVAFNVAGWVGLSVLGTVVLLWPAILHTRIEQSA
                     DQAARRALPVGALGILVVGAASLTGIRLLGSVGLLLYLAAVLLVLRECARQAKATPPT
                     SYAGWAMGTALLWFAGCIAALAVAVFVAGWPGIETVIAALLVPFVVGFVAQLISGALS
                     HLIPVTVGGGAASVRRMNAALDAQGAFRVIVVNGALVVALFPLPRLVHVVVVVVAVAT
                     LVAVIPLAVVAIRNSRRTDAPVAPRSAEERRATITLHSLSGSMFAAVGVLVLAVAVGV
                     SFSGEASFSTTGPGIIASSQGGSAASTAAEIKPTGKTTRVEMTMKDLRFWPSTVKVPA
                     GNKLVIHVTNKDDMVHDLTLATGQSSGRLVGGKSATVDVGVVTANINGWCSIPGHRQL
                     GMVMKIVAEGAAATKGGSHAGTTDHSSGGMAMAMGSGQGKSAASDIDLAKNPPASFTA
                     RDPQLPARTGKVHHITLTVRDVKTQVAPGITQDLWTYNGTAPAPIIQGKVGDRFDITL
                     INHGDMGHGIDFHAGELSPNGPMRTINPGQKLTFSFTAEHSGIWLYHCSTMPMSQHIA
                     NGMYGAVIIDPPGMDKVSKQFVFIQSEYYLGPQGGIADPKRIATQNPDLVVFNGYADQ
                     YKYRPITVKAGQRVRIWVLDAGPNVPADFHVVGTQFPTVFKEGNYLIQNGGVTKTGAS
                     QALGLMPAQGGFVELTFPAPGDYTFISHIMSDAEKGAMGVFHAVK"
     gene            complement(3755..5152)
                     /locus_tag="VFQ96_01250"
     CDS             complement(3755..5152)
                     /locus_tag="VFQ96_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256446.1"
                     /translation="MPTLSSDTPDGAKKNGGTATEPGPEPDGDDAASVSHVLSGHAGS
                     RARDLQNGFTIEAPSRLGGARAAGGVTLQRSALSRTSIRLLFLIPGAIALLSGLDAAL
                     LLLGVWSPVMTERLPVVHGPLLVFGFVGTVITLERAVAVRRWWGFGSPSFYGLGGILL
                     LTPLPIQVGQVLFGLGGVVLLFIYLAIWRRQKMLSSAIQSLGAFLAVASALLWIGGYD
                     ADALVPGMAGFMVLTIAGERVELARLVTLSKTTERTAFGVCAGQAVGVLMAMLWPAVG
                     YPIFGLSLIAVVAWLAVFDAAPRLAKLPGLPRYIALCLLAGYGWLIVAGLIWTVAGPV
                     TNGPWYDGAVHSVFLGFTIAMIMAHAPVILPAVLRRPLPYYPIFALPVILLNASLVLR
                     ILIGDLYSIPLMVQIGGIVNVIALLLFVIFSVISVVRGKQPARVPALPRTGVWAARSV
                     APSPAAVAPAPEEAS"
     gene            5358..5597
                     /locus_tag="VFQ96_01255"
     CDS             5358..5597
                     /locus_tag="VFQ96_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU0256447.1"
                     /translation="MSDRPVLDVRNEIPARRHQIIFEEFGKLQPGEGYVLVNDHDPKP
                     LYYQFAAEHAGDFTWEYLEQGPEVWRVDIGRPAAK"
     gene            5603..6115
                     /locus_tag="VFQ96_01260"
     CDS             5603..6115
                     /locus_tag="VFQ96_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256448.1"
                     /translation="MLAAAGGSEETASVYPTPLLALFLIVLLVATSVWIGGWFSLIVI
                     ARTTTATLSPQSRVAFFRHFGKVYGIASTVALVVALAFGLILLVGMPWTALSTWCAAL
                     SVFLIVVLIAGVLQARAQRRMRQQLIASPDQALQARTATGARAAAVLRAGLGVVSIAI
                     LVLAVVRMVS"
     gene            complement(6147..6545)
                     /locus_tag="VFQ96_01265"
     CDS             complement(6147..6545)
                     /locus_tag="VFQ96_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256449.1"
                     /translation="MAGFVANLDRAGNREPQGFAPRRLAKDPEQFGQNGDPLEPADDV
                     DPADPRFIAANLQHWVANSVREGVLLGRTNLATMLTSLTTTSPEMGYDRVLSTLRGYE
                     LMQLHDLVTWSTKYDSVRSIVTLVLNEHFR"
     gene            6673..7506
                     /locus_tag="VFQ96_01270"
     CDS             6673..7506
                     /locus_tag="VFQ96_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0256450.1"
                     /translation="MTESIPQVSLNSGTTIPQLGFGVFLVDPADTQRVVEDALEIGYR
                     HIDTATGYNNETEVGKAIAATGIRREELFITTKLSNDDHRAGDVRGGFERSLDRLGTG
                     YLDLYLIHWPLPGVDKYIDTWRTIQTFAADGRAKAAGVCNFTVAHLTRLLAETDLVPA
                     VNQVELHPAFAQRELQAFGTEHDIRTEAWGPLGQAKYDLFGLAPIRAAAAAHDVTGAQ
                     VVLRWHIQHGRIIFPKSTHRARLQENFAIFDFTLTDDEMAAIDGLDHGKRVGSDPDVV
                     N"
     gene            complement(7526..8056)
                     /locus_tag="VFQ96_01275"
     CDS             complement(7526..8056)
                     /locus_tag="VFQ96_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU0256451.1"
                     /translation="MTNPYDQIAVVPGFTLASPDIADGRELAREQVSGVLGAGGEDVS
                     PGLSWSGFPLSTKSFVVSVFDPDAPTGSGFWHWVVADLPVTVTSLPTGAGREEPGADL
                     PAPAWQLRGDSGHARYIGAAPPAGHGKHRYFTAVHAVDVPSLGVPHDASPAYLMFNLF
                     RHTLARASMAPWWEAH"
     gene            complement(8154..>8358)
                     /locus_tag="VFQ96_01280"
     CDS             complement(8154..>8358)
                     /locus_tag="VFQ96_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256452.1"
                     /translation="AQVMGAVPQLDWLHPWLFSRYWTGFTGLLTKPIDGGAFVKNLVL
                     QGGYVLVAGALAWSRLATKDIFD"
BASE COUNT         1334 a         2842 c         2953 g         1229 t
ORIGIN      
        1 gccgaggtcg gtgacctcga ccccggtctc gatcaggccg ccgtgcgcgg cgagatccgc
       61 cgtcagcgcc cgcacgatcg aggcgctgcc gccgtacggg atgggccagc cgcccgcgtg
      121 ggcgagcccg gtcaagacga ggccggccgc cgccgccccg agcccgggca gtgggccggt
      181 cgtgtgtgcg aagaccccgg cgagaagcgc cggggcgtcg tcgccgcgaa acccgcccgc
      241 ccggccgcgg atgccgtgcc gcgccacgcg ggccgcgaaa cgcagcagga aggcggggtc
      301 gtcgggcacg cgccacgccg gtcggcctgc caggttccag agccgcgccg gctccgcggc
      361 cagcgggccg aggagccggc gccacgcgtc gccgtcttca ccgagtgcgt cggccgtgcg
      421 gtcgaggctc cgccaggcaa gcgccgcatg ggccggcgtg acggcctgcg cgtaggagac
      481 ctccggcgtc aggaaccgga cgcgctcggt gatgtgaaac cggcggaaga attcgcccgc
      541 caatgcgagc ggatgcaccg ccgagcagac gtcgtgccag acgccaggaa cgaggagctc
      601 gcgggtggcc gcccctcccc cgacgtggtc ggaacgttcc acgacgacga cccgcaggcc
      661 ggcccgcgcg agggtgacgg ccgccgcgag gccgttcggg cccgacccga cgacgatcgc
      721 gtcggcgtcg cccccgcgcg acgccccggc acccatgggt cgagactatc ggggcggcac
      781 gggcgcggcg ggcgtctggc cggaggatca cgatgggttc atggatcccg cgacccgccg
      841 cctgatgttc tggctgaagg cgccgtacct ggtcgatgtc gcgctcggcc tcgtcggtgc
      901 ggcgcttctc gccgcggggc gcgcggcggg ctggtgggtc atcctcttcg gggccggccg
      961 ggccgcgctg gcgaccgccg cgcttatctg gctgaccccg cggctccggc gccgaagcgc
     1021 acccggttcc ggcgcggggg cggcccggca ggtcacttca cggcgtggaa gacgcccatg
     1081 gcgcccttct cggcgtcgga catgatgtgg ctgatgaagg tgtagtcgcc cggcgccggg
     1141 aaagtgagct cgacgaagcc gccctgggcc ggcatgaggc cgagcgcctg ggaggccccg
     1201 gtcttcgtga cgccgccgtt ctggatgagg tagttgccct ccttgaagac cgtggggaac
     1261 tgggtgccca cgacatggaa gtcggcgggc acgttcggcc cggcgtcgag cacccatatt
     1321 cgcacgcgct gacccgcctt cacggtgatc gggcggtact tgtactggtc ggcgtagccg
     1381 ttgaagacga cgaggtcggg gttctgcgtc gcgatgcgct tcgggtcggc gatgccgccc
     1441 tgcgggccca ggtagtactc cgactgaatg aagacgaact gcttgctgac cttgtccatg
     1501 ccgggtgggt cgatgatgac cgcgccgtac atgccgttcg cgatgtgttg cgacatgggc
     1561 atggtcgagc agtgatacag ccagatgccg ctgtgctccg cggtgaagct gaaagtcagc
     1621 ttctgacccg ggttgatggt gcgcatcggc ccgttcggcg agagctctcc cgcatgaaag
     1681 tcgatgccgt ggcccatgtc gccgtggttg atgagggtga tgtcgaagcg atccccgacc
     1741 ttgccttgga tgatgggcgc cggtgcggtg ccgttatagg tccagaggtc ttgggtgatg
     1801 cccggcgcca cctgggtctt cacgtcgcgc accgtgaggg tgatgtggtg caccttgccg
     1861 gtcctcgccg gcagctgcgg gtcacgagcc gtgaaagagg cgggcgggtt ctttgccagg
     1921 tcgatgtcgg aggctgcgga tttgccttgc ccggagccca tcgccatggc catgccgccg
     1981 gaggaatgat cggtcgtgcc cgcgtgactg ccgcccttgg tcgccgctgc gccctctgcc
     2041 acgatcttca tgaccatgcc gagctgccgg tggcccggta tcgagcacca gccgttgatg
     2101 ttcgcggtca cgaccccgac gtcgacggtg gccgacttgc cgccgacgag ccgcccggaa
     2161 ctctgcccgg tcgccagcgt gaggtcgtgc accatgtcgt ctttgttggt gacgtggatg
     2221 acgagcttgt tgcccgcggg cactttgacg gtgctcggcc agaatcgcag atctttcatc
     2281 gtcatctcga cccgggtggt cttgcccgtc ggcttgatct cggccgcggt cgacgccgcg
     2341 ctgccgccct gtgaagaggc gatgatgccc ggcccggtcg tcgagaacga ggcttcgccg
     2401 ctgaacgaca ctccgacggc gacggcgagc accagcacgc cgacggcggc gaacatgctg
     2461 ccgctcagcg agtgcaaggt gatggtggca cggcgctcct cggccgagcg tggcgccacc
     2521 ggagcatccg ttcgcctgct gttgcgtatc gcgacgacgg cgagcgggat aaccgcgacg
     2581 agggtggcga cggcgacgac gacgacgacg acgtgtacga ggcgcggcag cgggaagagc
     2641 gccacgacga gcgcgccgtt cacgacgatg acccggaagg cgccttgcgc gtcgagcgcc
     2701 gcgttcatgc gccgcacgga ggccgcgccg cccccgaccg tgaccgggat gaggtggctc
     2761 aaggcgccgc tgatcagctg ggcgacgaag ccgacgacga acggcacgag aagcgcggcg
     2821 atcaccgtct cgatgccggg ccacccggcg acgaagacgg cgacggccaa ggccgcgatg
     2881 cagccggcga accagagaag ggccgtgccc atggcccaac cggcatacga ggtgggcgga
     2941 gtcgccttcg cctgccgggc gcactcccgc aagacgagca gcaccgcggc aaggtacagc
     3001 aggaggccta cgctgccgag caggcggatg cccgtcagag aggcggcgcc gacgacgagg
     3061 atgccgaggg cgccgaccgg cagtgcgcgg cgggccgctt ggtcggcgct ctgctcgata
     3121 cgggtgtgca ggatcgccgg ccagagaaga accaccgtgc cgagcacgct gaggcccacc
     3181 cagcccgcga cgttgaaagc gacgtgggcg agaagcagac ggccgtgcgc cgcctggccc
     3241 gcgtctgccc cggccatcac gactcccagg cagatgccga cgacgagcat gaggccggcg
     3301 acgacgtagt agcgcaagac gatcgagaag cgcgtggtcg tcgagcgaac ccgcaggtac
     3361 aacgccgcca cgtgccagac gatcgccacg gccacagccg ccgcgccggc gacgacgagc
     3421 ggccacaggc cgaacggcac cccgacgatg acggcgagcg tgcccggcgt gtgcagggcg
     3481 agtcgcgcgg caagcccacg gcgcccgtcg ccggcccggt ggatcagcgt gtcggcgaag
     3541 tattggctcc agatcatgat cgccgcggtc gcggcgccca gcagcacgag atgtacgctc
     3601 agccaggcgg tggcctggcc ccgccatggg atcacgcccg cgacggcggc cgcgagcgcc
     3661 caggcgagaa cgagcgagtc gaccctgcga taccaggccc gcctcgcgtt accatcggcg
     3721 ctgcgccctg tggtgcgtgc tgccttgtcg tcactcacga agcctcctcc ggtgccgggg
     3781 cgaccgccgc gggcgacggc gccaccgacc gcgcggccca gactcccgta cgcggcagcg
     3841 cgggcacgcg ggcgggctgt ttgccgcgca cgacggagat gacgctgaag atcacgaaca
     3901 ggagcagcgc gatgacgttg acgatgccgc cgatctgcac catgagcggt atcgaataca
     3961 ggtcaccgat aaggatgcgc aagacgagcg acgcgttcaa gaggatgacg gggagcgcga
     4021 agatcgggta atacggcagc ggccggcgca gcacggccgg cagaatgacg ggcgcgtgcg
     4081 ccatgatcat ggcgatcgtg aacccgagga acacgctgtg cacggccccg tcgtaccacg
     4141 gcccgttggt gacggggccc gcgaccgtcc agatgaggcc ggcgacgatg agccagccgt
     4201 agccggccaa gaggcatagg gcgatatagc gcggcaggcc gggcagcttc gcgaggcgcg
     4261 gcgcggcatc gaacacggcc agccatgcga cgacggcgat gagcgagagg ccgaagatcg
     4321 ggtagccgac tgccggccac agcatcgcca tcagcacgcc gaccgcctgg cccgcgcaca
     4381 ccccgaacgc ggtgcgctcc gtcgtcttgc tgagggtgac gagccgtgcc agttcgaccc
     4441 gctcccccgc gatcgtcagc accatgaacc ccgccatccc ggggacgagc gcatcggcgt
     4501 cgtagccgcc gatccacagg agggccgatg cgaccgcgag gaaggcgccc aacgactgga
     4561 tggcgctgga gagcatcttc tggcggcgcc agatggccag gtagatgaag aggagcacga
     4621 cgccgcccag gccgaacagt acctggccga cctggatcgg cagcggggtc agaaggagga
     4681 tgccgcccag gccgtagaag ctcggcgacc cgaagcccca ccaccgccgc acggcgaccg
     4741 cgcgctccag cgtgatgacc gtgccgacga agccgaacac gaggagcggg ccgtgcacca
     4801 cggggagccg ctcggtcatc accggcgacc agacgcccaa gagcaagagc gccgcgtcga
     4861 gcccgctcaa gagcgcgata gccccgggga tcaggaagag cagccgaatg ctcgtgcggc
     4921 tgagtgccga gcgctgcaag gtgacgccgc ctgccgcccg tgccccgccc agccggctgg
     4981 gcgcctcgat ggtgaacccg ttctgcaggt cacgggcccg ggagccggcg tgaccgctta
     5041 agacgtgcga gacggatgcg gcatcgtcgc cgtccggctc aggcccgggc tcggtggctg
     5101 tgccgccgtt ttttttcgcc ccgtcgggcg tgtcggaaga caatgtaggc acgtgagagg
     5161 gctcgacggg aaccgacgtc tccgccgttt cggtcgaggg tcggggccgt ggctgggggg
     5221 atgccgcggc tcggtcgtgt ccttcgatct cgtccccgtc tcgctggtct ctcacgtgcc
     5281 gacgcacctc attttattac tttttattgg atgggccatg ctagtttcaa cagtgtcctt
     5341 cgataagaga gtgaatcatg agcgatagac cagttctcga cgtccgtaac gagatccccg
     5401 cgcgccgcca ccagatcatc ttcgaggagt tcggcaagct ccagcccggc gagggctatg
     5461 tgttggtcaa cgatcacgac cccaagcctc tttattacca gttcgccgcc gaacacgccg
     5521 gtgacttcac gtgggaatac cttgagcagg gcccggaggt gtggcgcgtc gacatcggtc
     5581 ggcccgctgc gaagtgagga tcgtgcttgc cgccgcgggc ggcagcgagg agacggcgtc
     5641 ggtgtatccg acgccgcttc tcgcgctttt tctcatcgtc ttgctcgtgg cgacgagcgt
     5701 gtggatcggc ggctggttca gcctcatcgt catcgcacgc acgacgacgg ccacactttc
     5761 gccacaaagc cgcgtggcgt tcttcaggca cttcggcaag gtctatggca tcgcgtcgac
     5821 ggtcgcgctt gtcgtcgctc tcgcgttcgg cctgatactg ctcgttggca tgccatggac
     5881 ggcgctgtcg acgtggtgcg cggcgctttc cgtgttcctc atcgtcgtct tgatcgccgg
     5941 ggttctgcag gccagggcgc aacgtcgcat gcggcagcag ctcatcgcct cccccgacca
     6001 ggccctgcag gcccgcacgg cgacaggcgc gcgcgccgcc gccgtcctgc gggcgggcct
     6061 cggcgtggtg agcatcgcga tcctcgtgct cgccgtcgtg cgcatggtgt cgtaaggccg
     6121 ccagccgccc ggtcggcagc cgcggttcag cggaagtgct cgttcaagac cagcgtcacg
     6181 atcgagcgca cggaatcgta cttggtgctc caggtgacga ggtcgtgcag ttgcatgagc
     6241 tcatagccgc gcagcgtgct gagcacccgg tcatagccca tctcgggtga cgtcgtcgtc
     6301 aacgaggtca gcatcgtggc cagattcgtg cggccgagca gcacgccctc ccgcaccgag
     6361 ttcgccaccc agtgctgcag gttcgccgcg atgaaacgcg ggtcggcggg gtcgacgtcg
     6421 tcggctggct ctaacgggtc tccgttctgg ccgaactgtt cgggatcctt ggccaaccgg
     6481 cgcggggcga aaccctgcgg ctctcggttt cccgcccgat cgagattcgc gacgaatccg
     6541 gccaccttcc tcctcctccc gcgggccgcc aaggcctgcc gtcgggaccc ggagtcccgg
     6601 ggcccacggt taaaaggata cggaggaacg ccctgatcgc cgacgcggca tgcgggcggt
     6661 tagcattcga ccatgaccga atcgattccg caggtttccc tcaactccgg cacgacgatc
     6721 ccccaactag gcttcggagt ctttctcgtc gatcctgccg acacccaacg cgtggtcgaa
     6781 gacgcgttgg agatcggcta tcggcacatc gacacggcca ccgggtacaa caacgagacc
     6841 gaggtcggca aggcgatcgc cgcgaccggc atccgccgcg aggagttgtt catcaccacc
     6901 aagctcagca acgacgacca tcgggccggc gacgtgcgag gtgggttcga gcgcagcctc
     6961 gaccggctcg gcaccggtta cctcgacctg tatctcatcc attggccgct gccgggggtg
     7021 gacaagtaca tcgacacgtg gcgcaccatc cagaccttcg cggccgacgg gcgcgcgaag
     7081 gcagccggtg tgtgcaattt cacggtggcc cacctgacga ggcttctcgc cgagaccgac
     7141 ctcgtgccgg ctgtcaacca ggtggagctg catccggcat tcgcgcagcg ggaattgcag
     7201 gccttcggca ccgagcacga catccgcacc gaggcgtggg ggccgctcgg ccaagcgaag
     7261 tacgacctct tcggcctcgc accgatccgg gcggcggccg ccgcacacga cgtcacgggc
     7321 gcgcaggtcg tgctgcgctg gcacattcag cacggccgca tcatcttccc gaagtcgacg
     7381 caccgcgcac ggctccaaga gaacttcgcg atcttcgact tcacgctgac cgacgacgag
     7441 atggccgcca tcgacggcct cgaccacggc aagcgggttg gcagcgaccc ggacgtcgtc
     7501 aactgacgcc ccggccggct cgagctcagt gcgcctccca ccagggggcc atcgacgccc
     7561 tcgccagcgt gtggcggaaa aggttgaaca tgaggtaggc ggggctcgca tcgtgcggca
     7621 cgccgaggct cggcacgtcg acggcgtgca cggcggtgaa gtagcggtgc ttgccgtgac
     7681 cggcaggcgg ggccgcaccg atgtagcgcg cgtgcccgct gtcgccgcgc agctgccagg
     7741 ccggcgcggg caggtcggcg cccggctcct ccctgcccgc gccggtcgga agactcgtca
     7801 cggtgacggg caggtcggcg accacccagt gccagaagcc gctgcccgtc ggcgcgtcgg
     7861 ggtcgaagac cgagaccacg aagcttttcg tcgacagggg aaagccagac cacgacagcc
     7921 ccggggagac gtcttcccca cccgcaccca ggacgccgct gacctgttcc cttgcgagct
     7981 ctcggccgtc ggcgatgtcc gggctcgcca aggtgaatcc ggggaccacg gcgatctggt
     8041 cgtacgggtt tgtcacggga gcctccctcg ccttgcccgc cggtttccgc gggccctcgg
     8101 ctttgagact acgcgtgcgc cgggaggggc gttacggccg cttcgggtgc gtgtcagtcg
     8161 aagatgtctt tcgtcgcgag gcggctccac gccagggcgc cggcgacgag cacgtagccg
     8221 ccctgcagca cgaggttctt gacgaaggct cccccgtcga tgggcttcgt caagagcccg
     8281 gtgaagccgg tccagtaacg gctgaagagc cacgggtgca gccagtcgag ttgcggcacg
     8341 gcgcccatca cctgcgcg
//
LOCUS       DASTNL010000043         6724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1329906, whole genome shotgun sequence.
ACCESSION   DASTNL010000043 DASTNL010000000
VERSION     DASTNL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329906"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /gene="atpA"
                     /locus_tag="VFQ96_01285"
     CDS             complement(<1..1451)
                     /gene="atpA"
                     /locus_tag="VFQ96_01285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0256453.1"
                     /translation="MAELTISPEEIRAALQDFVGSYEAKRASATEVGHVIDAADGIAH
                     VEGLPGVMANELIRFANGVEGLAQNLDETEVGAIVLGEFGGIVEGMEVTRTGEVLSVA
                     VGDGYLGRVVDPLGNPIDGLGEVKTETRRALELQAPGVMQRKSVHEPLQTGIKAIDAM
                     IPIGRGQRQLIIGDRQTGKTALAVDTIINQKANWESGDPNRQVRCIYVAVGQKGSTIA
                     SVKGALEDAGAMEYTTIVAAPASDPAGFKYLAPYTGSAIGQHWMYAGKHVLIVFDDLS
                     KQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGAGSMTGLPIIETK
                     ANDVSAYIPTNVISITDGQIFLQSDLFNANQRPAVDVGISVSRVGGDAQLKSIKSVSG
                     TLKIELAQYRALQAFAMFASDLDAASRRQLDRGARLTELLKQPQYSPYPIEEQVASIW
                     AGSNGKMDDLPVEEVLRFEAEMLEHLRRNTTLLT"
     gene            complement(1516..2307)
                     /locus_tag="VFQ96_01290"
     CDS             complement(1516..2307)
                     /locus_tag="VFQ96_01290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU0256454.1"
                     /translation="MGSATRHAMTAAVGAIKDLDGVDIEVARQVFGVGRVIASSRALR
                     GVLADASVPGEAKEALVHKLLPDFLEPRAGRVVGVALGQRWSHDDDLVSGLEELGVRL
                     AAEAGDAERVQRELFSFGRAVASSPALELALDDKVGQADEKITLIDALLAGRASEASA
                     VILREVVGHARGRSARSAIESAARVVADERGQVVATVTVAATLPGRRREGVAAHLARQ
                     YGRDVTVNYVIDPAIVGGMRIRVGDEVIDGTVTSKLTDARLKLAG"
     gene            complement(2307..2873)
                     /locus_tag="VFQ96_01295"
     CDS             complement(2307..2873)
                     /locus_tag="VFQ96_01295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU0256455.1"
                     /translation="MIHAMRVLAAAENTNPLVPAPADILWSTVCFLVILFFFWKVGIP
                     RLTKLLDERSAAIEGNVAKADEAQVKAEALLEEYTAQLAEARAEAARIREQAREEGKR
                     ILAELKEQANAEAERVTTAAQAQIDAERHAALVSLRSEVGSLALDLASGIITQKLQDD
                     ETASVVVERFLAELEDEQPVGTPAGSAD"
     gene            complement(2892..3125)
                     /gene="atpE"
                     /locus_tag="VFQ96_01300"
     CDS             complement(2892..3125)
                     /gene="atpE"
                     /locus_tag="VFQ96_01300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU0256456.1"
                     /translation="MDATTVLAAISGNIGTVGYGLAAIGPGIGIGIVAGKTVEAVARQ
                     PELSGRLQVLMYIGIAFTEALAFIGIATFFFMR"
     gene            complement(3171..3965)
                     /gene="atpB"
                     /locus_tag="VFQ96_01305"
     CDS             complement(3171..3965)
                     /gene="atpB"
                     /locus_tag="VFQ96_01305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU0256457.1"
                     /translation="MNLLTRTADIPPFQGPGIGEFFPPAVLFDGTWFAINRVMLVRFA
                     VVVAIVLLFWIGTRRMKVVPGRFQSVIEMGMDFVRVSLAEDILGKKDGRRFLPLIMAV
                     FFTVLIMNFAGVVPGMNVAGTAVIGLPGLMTLFAYVAFIYAGCRRSGWGFFRNALFPP
                     GVPWFFYPVISPLEFISTFLVRPFTLTLRLMMNMIVGHLLIVLFFSATQWFFFSVPGI
                     LKVIGVGTLAFGFAFTVFELLIIVLQAYIFALLTTVYIQLALLEEH"
     gene            complement(4140..4646)
                     /locus_tag="VFQ96_01310"
     CDS             complement(4140..4646)
                     /locus_tag="VFQ96_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256458.1"
                     /translation="MTTSEAAGPQPPDGQSGRPAMPTSNPVLARILRWGGLLAAAIAL
                     AGAIAGGVLAGGPGVAGGLLGAVMAAVFVAVTAASILVANRFIGSELYGAFFFAIVLG
                     GWIVKFVVFIVLLALLRDRPWLNGTVMFVTLVAGVVGSLVIDVVVVKTSRIGYASDTH
                     LPDASSGT"
     gene            complement(4643..5824)
                     /locus_tag="VFQ96_01315"
     CDS             complement(4643..5824)
                     /locus_tag="VFQ96_01315"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU0256459.1"
                     /translation="MTLLLVLTVVSAVITFGLSVAVWKISVHFHLYLGIRERDMHTHP
                     TPRLGGIAMFIGVLGAFGVALALAARHPVLNVVFADPTPVLAVLGGAFLIVVMGVVDD
                     LWDLDWMTKLAGQFIAAGVVAWLGVQIYSLPIGGAITVGSSWMSLAITVFAIVLVMNA
                     VNFIDGLDGLVAGVALIANAAFLVYVYLLTRQISPTNYFSLSAVIAAILVGACAGFLP
                     LNWHPARLFMGDAGALLIGMLMAVSAISVTGKINPSTIQGLGTGQLFPVFLPIILPFA
                     VLVVPLLDFTMAVLRRLRAGKSPFSADRKHLHHRLIDMGHSTLGAVVVFYSWTSVVSV
                     GCLLFFFRPYWIGVLFLVVGTLVCAFVTLAPLLRRRWRERTARRENRRTLPTALKEAQ
                     R"
     gene            complement(5821..6513)
                     /locus_tag="VFQ96_01320"
     CDS             complement(5821..6513)
                     /locus_tag="VFQ96_01320"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0256460.1"
                     /translation="MSETFDCSESDALLAGMRRARAALGRGELVVLPTDTVYGVAADA
                     FTPRAVAALLAAKGRGRQAPPPVLIPDLPTLDALAQNVPDEVRRLVSEFWPGGLTVVL
                     PARPSLAWDLGETRGTVALRMPDDTIALEILKETGPLAVSSANLTGEPAAGSAAEAEG
                     MLGESVAVYLEAGHRPARQGDSVVEQASTIIDATALAAGDGGIRILRHGAVSDDRIRA
                     IVGDLLEEPADP"
BASE COUNT         1201 a         2250 c         2289 g          984 t
ORIGIN      
        1 gtaaggaggg tcgtgttgcg gcggaggtgc tcgagcatct ccgcctcgaa acgcagcacc
       61 tcctcgacgg gcaggtcgtc catcttgccg ttgctgcccg cccagatgga agcgacctgc
      121 tcctcgatcg ggtacggcga atactgcggc tgcttcagaa gctccgtgag ccgcgcgcct
      181 cggtcgagct ggcggcggct cgccgcgtcg aggtcggagg cgaacatcgc gaaggcttgc
      241 agcgcccggt actgggcgag ctcgatcttg agcgttcccg agaccgactt gatggacttc
      301 aactgcgcgt cgccaccgac acgggagacg gagatgccca cgtcgaccgc cgggcgctgg
      361 ttggcgttga agaggtcgga ctgcaagaaa atctggccgt cggtgatgga gatcacgttc
      421 gtcggaatgt aggccgacac gtcgttggcc ttcgtctcga tgatcggaag ccccgtcatc
      481 gacccggcac ccagctcgtc ggagagcttg gcgcaacgct ccaggagcct cgagtgcagg
      541 tagaaaacgt cgcccgggta ggcctcacgc cccggcggcc gacgcagcaa gagcgacacc
      601 gcacggtacg cctcggcctg cttggagagg tcgtcgaaga cgatgagcac atgcttgccg
      661 gcgtacatcc aatgctggcc gatggcgctt cccgtatacg gtgcgaggta tttgaacccg
      721 gccgggtcgg aggcgggggc ggcgacgatc gtcgtgtact ccatcgcccc ggcgtcttcg
      781 agggcaccct tgacggaggc gatcgtggag cccttctggc cgacggcgac gtagatgcac
      841 cgcacctgcc tgttcgggtc gccggactcc cagttggcct tctggttgat gatcgtgtcg
      901 accgcgagcg ccgtcttgcc tgtctggcgg tcgccgatga tgagctggcg ctgaccccgg
      961 ccgatcggga tcatggcgtc gatcgccttg atgccggtct gcaacggctc gtgcacgctc
     1021 ttgcgctgca tgacgccggg cgcctgcagc tccagcgcac gccgggtctc cgtcttgacc
     1081 tcgccgagcc cgtcgatcgg gttgccgagc gggtcgacga cacggccgag atagccgtcg
     1141 ccgaccgcga cggagagcac ctcgcccgtg cgggtgacct ccatgccctc gacgatgccg
     1201 ccgaactcgc ccaagacgat cgcgccgacc tcggtctcgt cgagattctg tgccaggcct
     1261 tcgacgccgt tcgcgaaccg gatgagctcg ttggccatga cgccgggcag cccttcgacg
     1321 tgcgcgatgc cgtcggcggc gtcgatgaca tggcccactt cggtcgcact ggcccgcttg
     1381 gcctcgtacg acccgacgaa atcttgcagc gccgcacgga tctcttcggg gctgatcgtt
     1441 agttctgcca tctctcgttt ccttctcacg ggaccgatga tcccgactgt cgtcctgggt
     1501 cgcagcgccg ccgggtcagc cggccagctt gagcctggca tccgtcagct tcgacgtgac
     1561 tgtgccgtcg atcacctcat cgccgacacg gatgcgcatg ccgccgacga tggcgggatc
     1621 gatcacgtag ttgacggtaa catcgcggcc gtactgtcgc gccagatgag cggcgacgcc
     1681 ctcgcggcgc cggccgggca gcgtggccgc aacggtgacg gtagccacca cctggccgcg
     1741 ctcgtcggcg acgacgcggg cggccgattc gatcgcgctg cgggcgctgc ggccgcgggc
     1801 gtggccgacg acctcgcgca ggatgaccgc gctcgcctcg ctggcacggc ccgccaggag
     1861 ggcgtcgatg agcgtgatct tctcgtcggc ctggccgacc ttgtcgtcca gcgccagctc
     1921 cagggccggg gaagacgcga cggcgcggcc gaaggagaag agctcgcgct gcacacgctc
     1981 ggcgtcaccg gcctcggccg ccagcctcac gcccagctcc tccaggcccg agacgaggtc
     2041 gtcgtcgtgc gaccagcgct gcccgagcgc cacgccgacc acgcgaccgg cgcgcggctc
     2101 gagaaagtcg ggcaacagct tgtgcacgag ggcctctttg gcctcgcccg gcacggaggc
     2161 atcggccaga acgccgcgca acgcgcgact ggaggcgatg acccgcccga cgccgaagac
     2221 ctgccgggcg acctcgatgt cgacgccgtc gaggtctttg atcgcgccga ccgcggcggt
     2281 catcgcgtgc cgtgtggcgc tgcccatcag tcggccgatc cggccggggt gccgacgggc
     2341 tgctcgtctt ccaactcggc aaggaagcgt tcgacgacga cggaggccgt ctcgtcgtct
     2401 tggagcttct gcgtgatgat gcccgaagca aggtcgagcg cgagggagcc cacctcggag
     2461 cgcaacgaga cgagggcggc gtgtcgttcc gcgtcgatct gcgcctgcgc ggccgtcgtg
     2521 acccgctcgg cttcggcgtt cgcctgctct ttcagctcgg cgaggatacg cttgccctct
     2581 tcacgggcct gctcgcggat tctcgccgcc tcggcacgcg cctccgcaag ctgtgccgtg
     2641 tactcctcga gaagagcctc agccttcacc tgtgcctcgt cggccttggc gacgttgcct
     2701 tcgatcgcgg cggagcgctc gtcgagcagc ttcgtcagac gagggatgcc gaccttccag
     2761 aagaagaaga ggatgacgag aaagcagacc gtcgaccaga gaatatcggc cggcgccgga
     2821 acgagcggat tcgtgttctc cgccgcggcc aacacgcgca tcgcgtgaat catggtgtct
     2881 ccttagtcgg atcagcgcat gaagaagaac gtggcgatgc cgatgaacgc cagcgcctcg
     2941 gtgaacgcga tgccgatgta catcaagacc tggagccggc cggagagttc tggctgacgc
     3001 gccacggcct cgaccgtctt acccgccaca atgccgatgc cgatgcccgg gccgatcgcg
     3061 gcaagaccgt atccgaccgt gccgatgttg ccgctgatcg cagcgagaac ggttgttgcg
     3121 tccacgtatg tttccttcct tagtgcggcc aggcattcgc ctgcccgttg tcaatgctct
     3181 tcgagcaggg cgagctggat gtagacggtg gtgagcagcg cgaagatata tgcctgcagc
     3241 acgatgatca gcagttcgaa gacggtgaac gcgaatccga aagcgagggt gccgaccccg
     3301 atcaccttga ggatgcccgg caccgagaag aagaaccact gcgtggcgct gaagaacaag
     3361 acgatgagga ggtgccccac gatcatgttc atcatgagac gcaaggtgag ggtgaagggc
     3421 cgcaccagga acgtcgagat gaactcgagc gggctgatca ccgggtagaa gaaccacggc
     3481 accccgggcg ggaagagggc gttgcggaag aagccccaac cgctgcgccg gcacccggcg
     3541 tagatgaacg cgacgtaggc gaacagtgtc atcaggccag gcaggccgat cacggcggtg
     3601 cccgccacgt tcatgcccgg cacgaccccg gcgaagttca tgatgaggac ggtgaagaag
     3661 accgccatga tgagcggcag gaaccggcgc ccatccttct tgcccaggat gtcctccgca
     3721 aggctgaccc gaacgaagtc catgcccatc tcgatgaccg actggaacct gccgggcacc
     3781 accttcatgc gcctggtgcc gatccagaac agcaggacga tggcgaccac gacggcgaag
     3841 cgcaccaaca tgacgcggtt gatcgcgaac caggtgccgt cgaacagcac cgccggcggg
     3901 aagaactcac cgatgccggg gccctggaac ggggggatgt ccgcggtccg ggtaagcagg
     3961 ttcacggcgt gaataagcag cgctatctcc cggattcagg gcgtcgagat aaactcaaag
     4021 cggaaacgaa caagaattgc gcacggcgcg aatgcggacg gtgcggtatc gctgtcaact
     4081 ctacagtaat ctgctgcacc cgccgaatca tgccgtgtca atccgggccg gacgcccggt
     4141 catgtaccgc tggaggcatc cggcagatgc gtgtctgacg cgtagccgat tcgcgaggtc
     4201 ttgacgacca cgacatcgat caccaatgag ccgacgacgc ccgccacgag ggtcacgaac
     4261 atcaccgtgc cgttcaacca gggccggtcg cgcagaagcg cgagcaagac gatgaagacg
     4321 acgaacttca cgatccagcc gccgaggacg atcgcgaaaa agaaagcacc atagagctca
     4381 ctgccgatga accggttggc gacgaggatg cttgcggccg tgaccgcgac gaagacggcc
     4441 gccatcacag cgccgaggag gccgcccgcg acgcccggcc cgccggcgag gaccccgccg
     4501 gcgatcgcac cggccagagc tatcgccgcc gcaagcaggc cgccccagcg caggatcctc
     4561 gcgagcaccg gattcgaggt gggcatggcg ggtcgccccg actgcccgtc gggcggttgc
     4621 gggcctgcgg cctcggatgt ggtcatcgtt gcgcctcctt gagggcggtc ggcagggtgc
     4681 ggcgattctc acgccgcgcg gtcctctccc tccaccggcg gcgcagaagc ggggccaggg
     4741 tgacgaacgc gcaaacgagc gtgccgacga cgaggaacag cacaccgatc cagtagggcc
     4801 ggaagaaaaa gagcaggcag ccgacgctca cgaccgaggt ccacgaatag aacaccacga
     4861 ccgccccgag ggtcgaatgg cccatgtcga tcaggcggtg atgaagatgc ttgcggtcgg
     4921 cgctgaacgg gctcttgccg gcacgcaggc gccgcagcac ggccatggtg aagtcgagga
     4981 gcggcaccac caagaccgcg aagggcagga tgatcggtaa gaagaccggg aagagctgcc
     5041 ccgtgccgag gccctggatc gtcgacgggt tgatcttgcc cgtcacggag atcgccgaga
     5101 ccgccatcag catgccgatg agcagggcgc cggcgtcccc catgaacagc cgcgccggat
     5161 gccagttcaa cggcaggaaa ccggcgcacg cgcccacgag gatcgccgcg atcaccgcgg
     5221 agaggctgaa gtagttcgtc gggctgatct ggcgggtgag caggtagacg tagacgagga
     5281 acgcggcgtt ggcgatcaag gcgacgcccg cgacgaggcc gtcgagcccg tcgatgaagt
     5341 tgacggcgtt catcacgagc acgatcgcga agaccgtgat cgcaaggctc atccacgacg
     5401 agccgaccgt gatcgccccg ccgatcggca acgaatagat ctgcacgccg agccaggcga
     5461 cgacgcccgc ggcgatgaac tgaccggcga gcttcgtcat ccagtcgaga tcccacaggt
     5521 cgtcgacgac gcccatgacg acgatcagaa acgccccgcc gagcacggcg aggaccgggg
     5581 tcggatcggc gaagaccacg ttcagcacgg ggtgccgggc cgccagcgcg agcgccacac
     5641 cgaaggcgcc caagactccg atgaacatgg cgattccgcc gagccgcggc gtcgggtggg
     5701 tatgcatgtc ccgttcccgg atgccgaggt agagatgaaa gtgcacgctg atcttccaca
     5761 ccgcgacgga gagcccgaag gttatgacgg cggagacgac ggtgaggacg agcaggaggg
     5821 tcacggatcg gccggctcct ccagcaggtc accgacgatc gcgcggatgc ggtcgtcgct
     5881 caccgccccg tgtcgaagga tgcggatgcc gccgtcgccc gccgcgagcg ccgtggcgtc
     5941 gatgatggtc gaggcctgct cgacgacgga gtcgccctgc ctcgccggcc ggtgcccggc
     6001 ctccagatag acggcgaccg actcgcccaa catgccttcg gcctccgccg cgctgccggc
     6061 cgccggctcg ccggtgaggt tcgcggagga gacggccaac gggccggtct ctttcaagat
     6121 ctccagggcg atcgtgtcgt cgggcatccg caaggcgacc gtgcctcgcg tctcgccgag
     6181 gtcccaggcc aacgacgggc gggccggcaa gaccacggtc aggccgcccg gccagaactc
     6241 ggagacgagc cggcgcacct cgtcgggcac gttctgggcc aacgcgtcca gcgtcggcag
     6301 gtcggggatc agcaccggcg gcggcgcctg gcgcccgcgc cccttggcgg ccaacagcgc
     6361 cgcgaccgcc cggggcgtga aggcgtcggc agccaccccg tagacggtgt cggtcggcaa
     6421 gacgacgagc tctcccctac ccagcgccgc gcgggcgcga cgcatgccgg cgaggagggc
     6481 gtcgctctcg gagcagtcaa aagtctcgga catgacggcc taatgatact gtccgatccc
     6541 cggcgccttc ctgtcacgcc gtcagtcggc ggcctccggt caggcggccc ggcgcgcgag
     6601 ggtggcacgc tcgcgcccgg tcaggtcgcg gtatgtccgt gcgccacgcc agcctgccgc
     6661 ggcgaagatc tcccgcaccg cccgagcctg cagggcgccg tgttccacca cgacggcgcc
     6721 gccg
//
LOCUS       DASTNL010000044        18156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1147380, whole genome shotgun sequence.
ACCESSION   DASTNL010000044 DASTNL010000000
VERSION     DASTNL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147380"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VFQ96_01325"
     CDS             complement(<1..1404)
                     /locus_tag="VFQ96_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU0256461.1"
                     /translation="MRSLGITRQPGWIGGVCAGIAARLGIDAIIVRGIVVVFALFGGP
                     VLLLYAAAWLLLPDTEDRIHLERLVHGEFTPPVIGVGVLFLFALLPVGPAWWVGHHFG
                     LAGFWSGAWHILWMLIVLGAIVWFVVWAVHRTRAGGPGGPGSGWPGAAPPRAPGHPPF
                     APPFTGAAPSRGPASAPPSWEGSASAGPAAPASSPSPGSGAAPSGVAGAGAGGSAAFA
                     TRGFASAAPASPPTAPDAPDPADPAALAAWKLRQAEWKREYDAWRAGQAESAEAERAL
                     ARLRAAEQRHLRQEQNAARQRAWQARVRRTRSNPLYSVIAIGLSLVAGATVTLLLGAG
                     SWTPQAALAGVAVTLGVCGLAVVVNGFLGRRSGGAGGVAILAVIALVLGSPFAWVAGP
                     VVTGRSVSWSPSYSPATVERTVVGGSARLDLTHYFDGGSSETAGSGSQAGTVILRVVG
                     GPAEVIVPADASSRVTAR"
     gene            1651..2868
                     /locus_tag="VFQ96_01330"
     CDS             1651..2868
                     /locus_tag="VFQ96_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU0256462.1"
                     /translation="MDSRVPRYDRAPLLRPRKAIVGGVCSALATHLGFPVSVVRLAMV
                     VFALFGGSGVLFYLWLWAFVPLAPALTRVDAGAPVSRRAPVGALLLAAGTVVAGAAVV
                     LANSATTPFAAIVGATLLAGAAVTWSLAFDQTDATRSARYRTLVRTLGAALLILTGML
                     LLASHGARPDAVTAVLAVLMIVLGAGVAAAPSVMRLWTELMAERAGRVRQEQRAEIAA
                     HLHDSVLQTLALIQNRAGASSEVARLARAQERELRDWLFAGTAPAEADLATQLRDIAA
                     AIEIDYPARLDVVAAGESAGPVNPALVAASREAMLNAARHAGGEVSIYLESSREAFDV
                     FVRDRGPGFDLTALPADRLGVRESIVGRMSRAGGAATVRRGSGGIGAEVHLHLDRALR
                     KESPQESTGGGPA"
     gene            2865..3521
                     /locus_tag="VFQ96_01335"
     CDS             2865..3521
                     /locus_tag="VFQ96_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0256463.1"
                     /translation="MTRVLIVDDHSIFRSGLRTDLDETIEVVGEAADVDSAVARIAEL
                     VPEVVLLDVHLPGGGAGAGGGGAEVIRRSAALLGSTRFLALSVSDAAEDVVGTIRAGA
                     RGYLTKTSSGAEVSRAIAAVAAGDAVFSPRLAGFVLDAFGAVAGEQAVADDELDRLSA
                     REREVMRLIARGYSYKEVASDLYISVKTVESHVSAVLRKLQLSSRHELTAWALERKLL
                     "
     gene            complement(3572..3958)
                     /locus_tag="VFQ96_01340"
     CDS             complement(3572..3958)
                     /locus_tag="VFQ96_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256464.1"
                     /translation="MSVRASHARAFLRSAELVDQLGEDAGILGAGNVIGSLAVLAGIA
                     AVDAVCGAVLGERAAGQDHAEAIRLLAATHSGKAAVPPFRRLMDAKTETQYSSGMLTD
                     SRSATLLRSAAKIVAVMEGVLRLAQP"
     gene            complement(3991..4614)
                     /locus_tag="VFQ96_01345"
     CDS             complement(3991..4614)
                     /locus_tag="VFQ96_01345"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU0256465.1"
                     /translation="MDLSHPEYTLFGADKARVLRRLAMLDEPTSGRRIHQLSGVRTLR
                     TTQRILDELVRVGLVRSRVVGRANAYRANRNHVLWPVIEAAIGVPAATEARIAEILAE
                     DLGEQAVSTALYGSAARREAGPDSDVDIFAVWKDGAPAEHAADLVNSAATRIEEVTGN
                     PAQIFAVDEEELGRLVAQKAPLVESLRRDARNLTGQDLRDLIVRAAR"
     gene            4747..6006
                     /locus_tag="VFQ96_01350"
     CDS             4747..6006
                     /locus_tag="VFQ96_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0256466.1"
                     /translation="MSRLRWMNAAGGRWAQRGWMGAVRHNRRVHISERADGSAALNAI
                     ARVRGELTESGIAVTELSDSNPTRHGLLHPGVLQAVARHAGDAARYDPQPRGARSARE
                     ALAARFGGEADDYWLTASTSQSYAWLLAVLTDPGEAVAAPAPGYPLVAPLARLSGART
                     AEYRLHYAHPHGWVLDTASLAAAVASPGLRAVIAVNPGNPTGAYVGGTIDDIARACAP
                     RGLPLIADEVFAPFALDGTASSVAGERRIVTITLGGVSKLLCAPQLKAAWIRLEGPEE
                     ALPPLRAALDAVADTFLPVSGPIAAALPELLALAEPSVEATLRRLRTNLAETRRVFAG
                     TGFRVRRCDGGWNVIVDAPRAAVTGGPGSDGLAVRLLRHAHLAVHPGWFYDLARDGAL
                     VVSLLPEPEDFADRLARLRRAVEDLAA"
     gene            complement(6183..6824)
                     /locus_tag="VFQ96_01355"
     CDS             complement(6183..6824)
                     /locus_tag="VFQ96_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU0256467.1"
                     /translation="MARLVIFGGTGYSGGNIARDALRRGHTVVSYARHAPREPIDGVE
                     YRIGSVTDRDIVARAAADADELVVALRAASVEGGLAAHVPALVAAAVASGARLSFVGG
                     AGSSLVAAGGPRLVDTAGFREEWKPEALAHAAVLAALQAAPEGLDWFYVSPAGLYGAQ
                     VPGETTGAYRDGGDVLVVKDDGGSEISGTDFALAYVDEIERHAHPRRRFTVGH"
     gene            7214..8278
                     /locus_tag="VFQ96_01360"
     CDS             7214..8278
                     /locus_tag="VFQ96_01360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEU0256468.1"
                     /translation="MDPENRRDATRADVEAYYETGSGAEVTLRENEAAWSALRFRPHV
                     LRDVGTVTTRTTLFGATLDVPIGIAPSALHGLAHPEGECATARAANASGTLFVLSMRS
                     SRSVEEVAAAAGGAPWWYQVYATVDRSVALRLAANASAAGAQALVLTGDTPLVARKRR
                     AFPGVVPIRAHVANLRHRLTPPPTASDIRQDPTVTPSFIAELAKATGLPVLVKGVVRG
                     DDADRLIAAGAAGIVVSNHGGRQLDRTVATADALPEVVVAVAGRVPVLVDGGIRTGGD
                     VLAALCLGADAVLVGRPVLRALGRGGEAEVTRLLHGLQQELAEALALAGVRSPAETSP
                     DMVRVSPRPLGRAALPQRLS"
     gene            complement(8383..9075)
                     /locus_tag="VFQ96_01365"
     CDS             complement(8383..9075)
                     /locus_tag="VFQ96_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0256469.1"
                     /translation="MSGWNVAPIGRVAAPLREQVIEMMRDAILNFQLQPGQRLIEREL
                     IEHFGVSRTTIREALRDLASKGLVTVIPQKGAIVAAPSAEEAADLYDVRAHLESLAVE
                     RFVGRATAEQVARLRGAVDELGRIAKTDPSDTQGLLHAKDDFYATLIEGAGSKVLEQL
                     LASLQARVRVLRATSLHASGRPAESARELRAVMDAIERSDADTAAQLCERHIQNAATT
                     ALQALVAKESVG"
     gene            9223..9996
                     /locus_tag="VFQ96_01370"
     CDS             9223..9996
                     /locus_tag="VFQ96_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0256470.1"
                     /translation="MTLTARQQEIKDAFRAERGWWGEPWDTVLKIDPEFVAAYQEMYS
                     VPHKKSHLTPEFRELCYISINAAATHLYIPGILPHIKKALDLGASAEEIVEVLELAAT
                     LGIHTMNIGVPLLVEVLEERGRTGPAPLTQYQEDLKAEFTELRGYWHDFWNEMLELDP
                     EMFEAYTHFSTVPWRTGTLTPAEKELIYISYDIAATHLYVPGTKLHIRNALNHGATVG
                     QVLEVMEISSIIGIHDLTTAVPILARELARREDGEVEPD"
     gene            complement(10194..10988)
                     /locus_tag="VFQ96_01375"
     CDS             complement(10194..10988)
                     /locus_tag="VFQ96_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0256471.1"
                     /translation="MTNDKKGKVMALTARQQEVKDEFIRVRGTWGETWEAILTLDAEF
                     LHAYLNFSAVPWRKNHLDTKTKEFLYIAIDSAVTHLYGPGTRQHIKAALAAGATPQEI
                     MEVLECTATLSIHAMNVGVPVLTEILAERGIRTEPAALNDYQERLKADFTRNRGYWNP
                     TWDEMLELDPELFDAYTEYSSHPWKNGTLSPKMREFIYMAFDTSATHLYKVGLKLHIE
                     NALGYGASVGEILEVMEIAAVIGIHSVALGAPMLLEEAEAAGVSVS"
     gene            complement(10985..11725)
                     /locus_tag="VFQ96_01380"
     CDS             complement(10985..11725)
                     /locus_tag="VFQ96_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0256472.1"
                     /translation="MPPVEATDDAWSITPVGRVAAPLREQVVTSLRQAIFSFDLKPGQ
                     RLVERELIERLGVSRTTVREALRELISDGLVTVMPQRGAIVSIPSAEEAADLYEIRAV
                     LESLLVRHFVERVDEAGVARLSASVDEFERTVHDGSDVQEVLAAKDGFYAVLLEGAQS
                     STLEQLLAGLKARVHMLRATSLGQEGRAEEVVAELRATVEAIGRRDGAAAARLFADHV
                     HRASRTAMRALDHEQSVALAGAAKTSDE"
     gene            complement(11764..12660)
                     /locus_tag="VFQ96_01385"
     CDS             complement(11764..12660)
                     /locus_tag="VFQ96_01385"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0256473.1"
                     /translation="MADAPATVGFVGLGNMGAPMTARLVQAGHRVQAYDVSGKARAHA
                     AELGAVPASSLADAVAGASVVILMLPNSDIVEAVAGDAGFSAALTTETLVIDMSSSEP
                     MRTRALAERFHAKGIDYIDAPVSGGVSGATKGTLTIMVGCPPELFDRVTTVVEPLGRP
                     VRVGSVGSGHAIKALNNLLSATHLWVTSEAMVAGQRFGIDPAVMLSVFNSSSGRSGST
                     DNKWPNYILPKTYASGFGLRLMLKDMKIAAGLAEQLGAPTRLGADAIALWSAAAEGLE
                     PAADHTEVARWIQERADDIKPG"
     gene            complement(12653..13054)
                     /locus_tag="VFQ96_01390"
     CDS             complement(12653..13054)
                     /locus_tag="VFQ96_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0256474.1"
                     /translation="MTDKDMTHRETLERGIATRRAVLGDAHVDRSMAKVSDFSRPIQE
                     LVSEYCWGEIWSGDELPRRTRSLINLAMLTALNRGHELGAHVRGALNNGVTVAEIQAV
                     LMQTAIYVGVPAALESFRIAEKVMKEIDENG"
     gene            complement(13054..13452)
                     /locus_tag="VFQ96_01395"
     CDS             complement(13054..13452)
                     /locus_tag="VFQ96_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0256475.1"
                     /translation="MIVIHPEGQEGVAGHRRSNVTGKAYPYVTKPAGEGAVVNTVNFT
                     PGAHTFWHAHERGQILQVLAGRGLVQSEGGPVEVVRAGDTVWCPPGEKHWHGAAPDAY
                     MVHTAISLGVTSWADEVDDADYQDPEARKE"
     gene            complement(13449..14363)
                     /locus_tag="VFQ96_01400"
     CDS             complement(13449..14363)
                     /locus_tag="VFQ96_01400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0256476.1"
                     /translation="MTEKRIGFIGLGNMGGRMTGCIVRAGQEVLGFDTKTENIAAVGA
                     SPAASVAEVVENTDLVFLSLPNSHIIESIAYGDGGLVAHAHAGQVIVDLSTAAPSSTR
                     KIHDALFEKGANYLDAGISGGAARAEKADLTLMVGGNATVLDSVRAELGYFASNIFYI
                     GVSGTGHITKLLNNFLNAVSLSATAEVMVAAKKANLDLETVLKVLNTSSGVNFATTNR
                     FPKIIHGDYLKGGLTNSLMLKDITLYTELAAQLGVASLNSSGPVAAFGLARALGYADE
                     ISNTVVDAIGDVSGGVRLHEGTSQEAAR"
     gene            complement(14374..15210)
                     /locus_tag="VFQ96_01405"
     CDS             complement(14374..15210)
                     /locus_tag="VFQ96_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0256477.1"
                     /translation="MSERFRGKVAVVTGAGSGLGRASARRLAAEGANVVAVDVNARAV
                     DAVVEGLPTPSLAVEADISDEAAVERYMAAGVERFGHIDLYHLNAGIFGTFAALPDID
                     VAEFDRVVAVNVRGQFLGLRAAFRQFAAQSSTGSIVVTGSIAGITGSADLLPYTTSKH
                     AVHGLVHGAGAYGGPLGIRVNAVAPGIVPTELFADAATTTGGKNDMERRASTTPMRRA
                     GTPDEIAGVVAFLLSSDASYMAGQIVSVDGGATIVNTVRPAAGAGAWDPVAADRVIYG
                     SK"
     gene            complement(15207..16043)
                     /locus_tag="VFQ96_01410"
     CDS             complement(15207..16043)
                     /locus_tag="VFQ96_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU0256478.1"
                     /translation="MTSAGHDDYLVTIARHGTRVTARSDVFLNYPIYRQPDAPLGMDY
                     FVWVLESAERTIVVDTGYSRHGAQVRKRDVLVEVPELLRRLGVDPARVETVIITHAHY
                     DHIGNLDAFPAARFVLSRKEYEFWTGPNANQPLFHHSVEDDELTTLAGLVEAGRVDFF
                     DGSTRPAPGVEVIEVGGHTPGQSVVKVQTTQGTVLLASDAVHYYEELTNDMPFSSVAD
                     LVEMYDTFARIRAMLDSGEVKHVVAGHDPDTIGRFTPAAGALAGVAATIGTPVTTREA
                     RS"
     gene            complement(16040..16852)
                     /locus_tag="VFQ96_01415"
     CDS             complement(16040..16852)
                     /locus_tag="VFQ96_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU0256479.1"
                     /translation="MVTTAESHDGAVSVWSVKYGERVVRKSDVFHDYAQYGLPDGNLD
                     MDYNFWVIRSQGKIILLDTGYDIPKRDWLGEISVTPTPEGLQIIGIDPADVSMVITSH
                     YHYDHIGYLKLFANAQIVSAQVEYDYWIGKLHAGALEGEFSSEDDLRPVLEAEKQGRL
                     WLVTDETEVFPGITVYPIGGHCPGELLTKVQTAGGGLILTVDAAHFYEQIEHEWPFFA
                     FTSLDEMKRGLSFINELARTTGATVVPGHDARVRDRFPAAPGAAGEIATVLA"
     gene            complement(17045..17770)
                     /locus_tag="VFQ96_01420"
     CDS             complement(17045..17770)
                     /locus_tag="VFQ96_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0256480.1"
                     /translation="MAQKLLGKVAIVTGAAQGMGRAITRLFAEEGATVYAGDINAFEG
                     EAAPGVIPVHLDVANEGSWQGLLDRVSGEQQRLDVLVNNAGVIAYEPILDVTLESWNR
                     VMSVDMTGVFLGMRAGIPLMRDSGGGSIVNFSSIWGLAAVSGAHAYHAAKGAIANMTK
                     NVAVTHAADGIRANSVHPGHINTPLTERQAADVSDAVISKTAMKRAGEPIEVARAVLF
                     LASDDSSYVTGTQMVVDGGYLAQ"
     gene            complement(17834..>18156)
                     /locus_tag="VFQ96_01425"
     CDS             complement(17834..>18156)
                     /locus_tag="VFQ96_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEU0256481.1"
                     /translation="AGICGGISAMILLGRLGSATPTGGTGLELQVIAAVVIGGTSLFG
                     GKGSMLGTFFGVLLIGVINNGLTLINVSPFWVQFVQGALIFVAIVLDSFNTHRLARST
                     SRDN"
BASE COUNT         2842 a         6323 c         6135 g         2856 t
ORIGIN      
        1 ccgagccgtc acccggctgg aggcgtcggc gggcacgatg acctcggccg ggccgccgac
       61 gacccgcagg atcacggtgc cggcctgcga tcccgaccct gcggtctccg aagagcctcc
      121 gtcgaagtag tgggtgaggt cgagccgtgc cgagccgccc acgacggtgc gttccacggt
      181 ggccggcgag tagctcggcg accacgacac cgaacggccg gtgacgaccg gcccggcgac
      241 ccaggcgaag ggcgaaccga gcacgagggc gatcaccgcg aggattgcga cgccccccgc
      301 accgccgctg cgtctgccga ggaacccgtt gacgacgacc gcgagcccgc agacgccgag
      361 cgtgacagcg acaccggcaa gcgccgcctg cggcgtccag ctgcccgcgc cgagaagcag
      421 cgtgacggtt gcgccggcga cgagcgagag gccgatcgcg atgacggaat acagcgggtt
      481 ggagcgggtg cggcgcaccc tcgcctgcca cgcgcgttgc cgcgcggcgt tctgctcctg
      541 gcgcaagtgc cgctgttccg cggcacgcag ccgggcgagc gcgcgttccg cctcggccga
      601 ttcagcctgc ccggcacgcc acgcgtcgta ttcccgcttc cactcggctt ggcgcagctt
      661 ccatgcggcg agcgcggcgg gatccgccgg atccggggcg tcgggtgccg tcggcggtga
      721 ggccggcgcg gcggaggcga agccgcgagt ggcgaaggcg gcggagcccc ccgcgcccgc
      781 gcccgcgaca ccggaaggcg cggcgcccga gcccggcgac ggcgacgaag cgggagccgc
      841 cgggccggcg gaggccgagc cctcccagga cggcggcgcc gaggccggcc cccgggaagg
      901 tgccgcgccg gtgaacggcg gggcgaacgg cgggtggccg ggcgcccggg gtggtgccgc
      961 gccgggccat ccgctgcccg gcccccccgg gccgcccgca cgggtacgat gcacggccca
     1021 gacgacgaac cagacgatcg cgccgaggac gatgagcatc cagaggatgt gccacgcgcc
     1081 gctccagaac ccggcaagcc cgaagtgatg ccccacccac caggccgggc cgacgggcag
     1141 cagggcgaag aggaacagca cccccacccc gatcaccggc ggcgtgaact cgccgtgcac
     1201 gagccgttcc aggtggatgc ggtcctccgt gtcgggcagg agcagccagg ccgccgcgta
     1261 caacagcagc acggggccgc cgaagagggc gaacacgacg acgatgccgc gcacgatgat
     1321 cgcgtcgatc ccgagccggg ccgcgatgcc ggcgcagacg ccgccgatcc agccgggttg
     1381 gcgcgtgatg ccgaggctgc gcatccacca gaagaagcgg tgggcgggcg tcggccccgg
     1441 cgcgcgcggg cccctcgtcg ggggtccttg tggcggcggg ccctgcggtg gcgcatcccg
     1501 ggggggcgga tcctgcggcg ggggcgttcg ggtcgagtct gtcggcatga ttcgactgtg
     1561 cccgagccgg taccggcgcc gctatggggg aacaccctga ccgggccctg atttcctgtt
     1621 ccggggcggc ccggggtggc tccgatctgg ttggattcca gagtgccccg ctacgaccgc
     1681 gcgcccctct tgcgaccccg caaggcgatc gtcggcggcg tctgcagcgc gctcgccacc
     1741 cacctggggt tcccggtgag tgtggtgcgc ctggccatgg tcgtgttcgc cctgttcggc
     1801 ggctccggcg tgctgttcta cctctggctg tgggccttcg tgcctctcgc cccggccctg
     1861 acgagggtcg acgcgggggc gccggtcagc cgacgcgcgc cggtcggggc gctgctcctc
     1921 gccgccggca ccgtcgtcgc gggggccgcc gtcgtgctgg ccaattccgc gaccaccccg
     1981 ttcgcggcga tcgtgggcgc gacgctcctc gccggcgcgg ccgtcacctg gagcctcgcg
     2041 ttcgaccaga ccgacgccac gcgcagcgcc cggtaccgca ctctcgtgcg caccctcggc
     2101 gcggcgctgc tcatcctcac ggggatgctc ttgctggcct cgcacggcgc acggccggac
     2161 gcggtcaccg ccgtgctcgc ggtgctcatg atcgtgctcg gcgcgggcgt agccgccgcg
     2221 ccttccgtga tgcgcctctg gacggagctg atggcggagc gggccggccg ggtgcgccag
     2281 gagcagcgtg cggagatcgc cgcccacctg cacgactcgg tgctgcagac gctcgcgctc
     2341 attcagaacc gggccggcgc gtcgagcgag gtcgcgcgcc tggcgcgggc gcaagagagg
     2401 gagctgcgcg actggctctt cgccggcaca gccccggccg aggcggatct ggccacgcag
     2461 cttcgcgaca tcgccgccgc gatcgagatc gactatccgg cccgcctcga cgtggtggcc
     2521 gccggcgagt cggcggggcc ggtgaacccg gccctcgtcg ccgcctcccg cgaggcgatg
     2581 ctgaacgcgg cccggcacgc cggcggtgag gtctccatct acctggagag ctcgagggag
     2641 gcgttcgacg tgttcgtgcg cgaccgcggg ccgggcttcg acctgaccgc gctccccgcg
     2701 gatcgtctcg gcgtgcggga atccatcgtc ggccgcatgt cgcgggcggg gggcgccgcc
     2761 accgtgcgcc gcggctcggg cggcatcggc gccgaggtgc acctgcacct cgaccgcgcc
     2821 ctccgcaagg agagcccgca agagagtaca ggaggaggcc ccgcatgaca cgcgtgctca
     2881 tcgtcgacga tcactcgatc ttccgatccg ggcttcggac ggatctcgac gagacgatcg
     2941 aggtggtcgg cgaggcggcc gacgtcgact cggcagtcgc gcggatcgcc gaactggtgc
     3001 cggaggtcgt gctgctcgac gtgcacctgc ccggcggcgg ggcgggcgca ggcggcggcg
     3061 gggcggaggt catccggcgc agcgccgccc tgctaggctc gacgcgattc ctcgcgctga
     3121 gcgtctccga cgcggccgag gacgttgtcg gcacgatccg tgccggcgcc cgcggctacc
     3181 tcaccaagac cagctccggg gcggaggtca gccgggcgat cgccgccgtg gcggccggcg
     3241 acgccgtgtt ctcgccgcgc ctggccggtt tcgtgctcga tgccttcggg gccgtcgcgg
     3301 gggaacaggc cgtcgccgac gacgagctcg accggctctc ggcccgcgag cgcgaggtga
     3361 tgcggctgat cgcccgcggt tactcctaca aggaggtcgc ctccgacctc tacatctcgg
     3421 tcaagaccgt ggaaagccac gtctcggcgg tgctgcgcaa gctgcagctc tcctcccgcc
     3481 acgagctgac ggcatgggcc ttggaacgca agctgctgtg atccgccacc gcaatcgggt
     3541 ctgccgcgat ccggccctgc ccaccaccct gtcagggctg cgccaatcgg aggaccccct
     3601 ccatcacggc gacgatcttc gcggccgacc tgagtaacgt cgccgagcgg ctgtcggtga
     3661 gcatgccgga ggaatattgg gtctcggtct tcgcatccat gagccgacgg aacggcggga
     3721 cggcggcctt tcccgagtgc gtggcggcaa ggagtctgat cgcctccgcg tgatcctggc
     3781 ccgccgcccg ttcgcccagc acggccccac agacggcgtc gaccgccgcg atgccggcaa
     3841 ggacggccag ggagccgatc acattgccgg cgccgagtat ccccgcgtcc tcgccgagtt
     3901 ggtcgacaag ctccgcggac cgaaggaacg cacgcgcatg gctggccctg acactcacct
     3961 ccgcaggtga gatcggtctt gtgcgcgggg tcatcgtgcg gccctcacga taaggtctcg
     4021 gaggtcttgc ccggtgaggt ttcgggcgtc cctccgcagc gattcgacga ggggtgcctt
     4081 ctgggccacg agcctgccga gttcctcctc gtccacggcg aagatctggg cagggttgcc
     4141 ggtgacctct tcgatccgcg tggccgcgga attcacgaga tccgcggcgt gctcagcggg
     4201 cgccccatcc ttccagacgg cgaagatgtc gacatccgag tcggggcccg cctcacgccg
     4261 cgcggcagac ccgtagaggg cggtggagac ggcctgctct ccgaggtctt ccgccaggat
     4321 ctccgcgatg cgggcttccg tcgcggccgg caccccgatc gccgcctcga tcacgggcca
     4381 gagcacatga ttgcgattgg cccgatacgc attcgcgcga ccgacgaccc ggctcctgac
     4441 gaggccgact cgaaccaact catccaggat gcgttgcgtg gtccttaacg tccttacgcc
     4501 actgagctga tggatgcgcc ggcccgacgt aggctcgtcg agcatggcga gacgcctcaa
     4561 gactcgggcc ttgtcggcgc cgaacagcgt gtattcgggg tgggagagat ccaagacacc
     4621 tccaatcata tgactacata aacagtcata tgactatata tcgtcgtcat ttggctgacc
     4681 atcccgttcc cgtgaccgcg taagtcccgg aacgcaaatt agcttggcgc gtccacgccg
     4741 tcggtcatgt cgcgactccg gtggatgaac gccgcggggg gtcggtgggc gcagcgcggg
     4801 tggatgggcg ccgtgcggca caatcggagg gtgcacatct ccgagcgggc agacggttcg
     4861 gccgcgctca acgcgatcgc ccgggtgcgc ggtgagctta cggaatcggg catcgccgtg
     4921 acggagctct ccgattcgaa cccgacccgg cacggcctgt tgcacccggg cgtgctccag
     4981 gcggtcgccc gccacgccgg cgacgccgcc cgttacgacc cgcagccgcg cggcgcccgg
     5041 tcggcccggg aggcgctggc cgcccggttc ggcggcgagg ccgacgacta ctggctgacc
     5101 gcctccacct cgcagtcgta cgcgtggctc ttggccgtgc tgaccgaccc cggagaggcg
     5161 gtcgcagcgc ctgcacctgg ctacccgctg gtcgcgcctc tcgcccgact ctccggcgcc
     5221 cgcaccgccg agtaccggct gcactacgcg cacccgcacg gctgggtgct cgacacggcg
     5281 tcgctggctg ccgccgtcgc gtcgcccggc ctccgggcgg tcatcgcggt caacccgggg
     5341 aacccgacgg gcgcctacgt gggcggcacg atcgacgaca tcgcccgggc gtgcgcgcca
     5401 cgcggcctgc cgctcatcgc cgacgaggtc ttcgcgccgt tcgcgttgga cggcacggcg
     5461 tcgagcgtgg cgggtgagcg gcgaatcgtg acgatcacgc tcggcggggt ctcgaagctg
     5521 ttgtgcgcgc ctcagttgaa ggcggcgtgg atccgactgg aggggccgga ggaggcgcta
     5581 cccccgctgc gtgcggccct cgacgccgtg gccgacacgt tcctgccggt cagcgggccg
     5641 atcgcggccg cgctgccgga gctgctggcg ctcgcggagc cctccgtcga ggcgaccctg
     5701 cggcgactgc gcacgaacct cgccgagacg cgccgggtgt tcgccggcac gggcttccga
     5761 gtgcgccgct gcgacggcgg atggaacgtc atcgtcgacg cgccgcgtgc cgccgtgacc
     5821 gggggccccg gcagcgacgg cctcgccgtg cgcctcttac gccacgccca tcttgccgtg
     5881 caccccggct ggttctacga cctggcccgc gacggcgcgc tcgtcgtctc gctcctgccg
     5941 gagccggagg acttcgccga ccggctggcc cgcctgcgcc gagccgtcga agacctcgcc
     6001 gcctgacgtc ggcacaccgt cacggtcgcc gtggcacacc gtcaccaccc ttctgccgcc
     6061 gttgcccata gccacagcac gactgcggta cacgccgggg ccccgatcgg cagagagtcc
     6121 gagcagacgg ggccgaggat atgacgggcc gacggtcgcc gacgcagtca cgccagagcc
     6181 gatcagtggc cgacggtgaa tcggcggcgc gggtgggcgt gccgctcgat ctcgtcgacg
     6241 taggccagcg cgaaatcggt gcccgagatc tcggagccgc cgtcgtcttt gacgacgagc
     6301 acgtcgccgc cgtcccggta ggctccggtc gtctcgcccg gcacctgcgc cccgtagagg
     6361 ccggcagggc tgacgtagaa ccagtcgagg ccctcggggg cggcctgcag ggcagcgagc
     6421 acggcggcat gggcgagggc ctccggcttc cattcctccc ggaatccggc agtgtccacc
     6481 aggcgggggc ctccggcggc gacgagggag gagcccgcgc cgccgacgaa ggagagccgg
     6541 gctcccgagg ccacggccgc cgcgacgagt gccggcacgt gggcggccag gccgccctcg
     6601 accgaggcgg cgcgcagtgc gacgacaagc tcatccgcat cggcggcagc acgggcgacg
     6661 atatcgcggt cggtgacgga cccgatgcgg tactcgaccc cgtcgatcgg ctcccggggc
     6721 gcatggcgag cgtaggagac gaccgtgtgg ccgcgccgca acgcatcgcg ggcgatgttc
     6781 ccgcccgaat acccggtgcc gccgaagatc accagtcttg ccatcgctct actccccttg
     6841 ctccccgtcg ccctggagga caccatcggc gtcccggacc ctcccgatcg agggcaacgg
     6901 cggcacgggc cgccttattc cgcggccttc ggcaccgaag ctcgcacggc gcaagcgcga
     6961 gaacgacgag actctgcgat catgaccgca tgtacgacga ccgaaggcac ccgtctaccg
     7021 gcccgccgca ccggtcgtgt cagggtgctc cgcgcttcat ctcccgcttc cgggcaacag
     7081 gttcgtgacc cgtggtctat ggccggcggc ccgtgcgcgc atgcccgcaa tcctcaccgc
     7141 cgtgacagcg gccgacggat gccgacgggg cggggcggcg cggccgtgga gaaaggaaga
     7201 acagaatggt gccatggatc cggaaaatcg ccgcgatgcg acgcgggcgg atgtcgaggc
     7261 gtactacgag accggttccg gcgcggaggt gacgctgcgg gagaacgagg cggcgtggtc
     7321 ggcactgcgg ttccggccgc acgtgctgcg cgacgtcggc acggtcacga cccgaacgac
     7381 cctcttcggc gccaccctcg acgtgccgat cggcatcgcg ccgagcgcgc tacacggcct
     7441 cgcgcacccc gaaggcgaat gcgccaccgc ccgggcagca aacgcctcgg gcacgctgtt
     7501 cgtgctctcc atgcgctcct cccgttcggt ggaagaggtc gcggcggccg caggcggcgc
     7561 gccctggtgg tatcaggtgt atgcgacggt cgaccgttcc gtcgccctgc gtctagcggc
     7621 caatgcgtcg gcggccggcg cgcaggcgct cgtcctcacc ggggacaccc ccctcgtcgc
     7681 ccgcaagcgc cgcgcctttc ccggcgtggt gccgatacga gcgcacgtgg cgaacctgcg
     7741 acaccgcctc actcccccgc cgaccgcgag cgacattcgg caagacccca cggtgacgcc
     7801 atcgttcatc gcggagctgg ctaaggcgac gggcttgcct gtgctcgtga agggcgttgt
     7861 acgcggcgac gacgccgacc ggctgatcgc cgccggggcc gccggcatcg tcgtctccaa
     7921 ccacggcggc cggcaactcg accgcacagt cgcgacggct gacgcgctgc ccgaggtggt
     7981 cgtcgccgtc gccggccgcg tgccggtact cgtcgacggg ggtatccgca ccggcggcga
     8041 cgttttggcg gcgctctgcc tgggggccga cgccgtgctt gtcggcaggc ccgtcttgcg
     8101 ggccctcggg cgaggcggcg aggcggaagt cacgcggctc ctgcacgggc tgcagcagga
     8161 gcttgccgag gcgctggcgc tggccggcgt gcgcagtccc gccgagacct ctcccgacat
     8221 ggtccgggtc tcgccccgac ccttggggcg agcggccctc ccgcagcggc tatcgtagga
     8281 caccgcgccc gcggaacgtg gtgtggctca cggcctacga cagacaggac cccgccgggc
     8341 ggcggcacag ggaaggtgcg aggcccagcg ggtcaggtca ggtcagccga cgctctcttt
     8401 ggcgacgaga gcctgcaagg ccgtcgtcgc cgcattctgg atgtgccgct cacacagctg
     8461 tgcggccgtg tccgcatcgg aacgctcgat cgcatccatg acggcgcgca gctcgcgcgc
     8521 cgactccgcc gggcgcccac tggcgtgcag cgacgtcgcc cggagcaccc gcacgcgggc
     8581 ctgcagcgag gcgagcaact gctcaagaac cttgctgccg gcgccctcga taagggtcgc
     8641 gtagaaatcg tctttggcgt ggagcagccc ctgcgtgtcg ctcgggtcgg tcttggcgat
     8701 acgacccaac tcgtcgacgg cgcctcgaag cctcgccacc tgctccgcgg tggcacgccc
     8761 aacgaagcgc tcgaccgcga gggattcgag gtgcgcacgc acgtcataga gatccgccgc
     8821 ctcttcggcc gagggcgccg cgacaatggc gcccttctgc gggatcaccg tgacgaggcc
     8881 ctttgaggcg aggtcgcgaa gagcctcacg aatcgtcgta cgcgaaacgc cgaagtgttc
     8941 gatgagctcg cgttcgatga gccgctgacc gggctgaagc tgaaaattga ggatggcgtc
     9001 gcgcatcatc tcgatcacct gttcgcgcaa cggcgccgcg acgcgcccga tcggcgcgac
     9061 attccaacca ctcaacttcg tctgccctgt cgacaacggc gaacacctcc cgcatcctgt
     9121 cagccaagta tatgatcata cactttgata acaatgagag ccgttgcgtt gtttgtatga
     9181 cggtcatatg ataggaggat ggtcgagcca aagggggcgc acgtgacatt gacagcgcgg
     9241 caacaagaaa tcaaagatgc cttccgagcc gagcgcggct ggtggggtga gccgtgggac
     9301 acggtgttga agatcgatcc cgagttcgtc gccgcctacc aagagatgta ttcggttccg
     9361 cacaagaagt cacacctcac tcccgagttc cgcgaactct gctacatctc gatcaacgcc
     9421 gcggcgacgc acctctacat ccccggaatc ctgccccaca tcaagaaggc actcgacctg
     9481 ggagcgagcg ccgaagagat cgtcgaagtg ctcgaactcg ccgcgaccct cggcatccac
     9541 acgatgaaca tcggggtgcc cttgctcgtc gaggtcctag aggagcgcgg ccgcaccggc
     9601 ccggcgccgc tgacccaata ccaagaagac ctcaaggcgg aattcacgga gctgcgcggc
     9661 tactggcacg acttctggaa cgagatgctc gagctcgacc ccgagatgtt cgaggcctac
     9721 acccacttct ccaccgtgcc gtggcgcacc ggaacgctga cgccggccga gaaggagctc
     9781 atctacatca gctacgacat cgcggccacg cacctctacg tgccgggcac caagctgcac
     9841 atccgcaacg ccctgaacca cggcgccacc gtcggccaag tgctcgaggt gatggagatc
     9901 tcgtcgatca tcggaatcca cgacctgacg actgctgttc cgatcctcgc ccgcgagttg
     9961 gcgaggcgtg aagacggcga ggtcgaaccc gactgaacca caccgccccg ccgcgtcgcc
    10021 gcagggtcgg ggtgcgccgg gtgtcgtact caagccagat tcgcggcggg gcgcgaggaa
    10081 gaggcagaga aaaaggaaaa agggagcgaa agaggaggag gggcgacccg gtggtcgccc
    10141 ctcctcctct ttcccgtact gacgcctttt tatcgcacgc cggccgaacg cgcctaagac
    10201 accgagacgc cggccgcctc cgcctcttcg agaagcatcg gcgcgcccaa cgcgaccgag
    10261 tggatgccga taacggccgc gatctccatc acctcgagaa tctcgcccac gctcgcgcca
    10321 tagccgagcg cgttctcgat atgcagcttg aggcccacct tgtacaggtg ggtcgccgag
    10381 gtgtcgaagg ccatgtagat gaactcacgc atcttcggcg aaagcgtgcc atttttccac
    10441 ggatgtgagg agtactcggt gtatgcgtcg aaaagctccg ggtcgagttc gagcatctcg
    10501 tcccacgtgg ggttccaata gccgcggtta cgcgtgaagt cggccttcaa tcgctcctga
    10561 tagtcgttca gggcggccgg ctcggtgcga atacccctct cggccaggat ctcggtcagc
    10621 accgggacgc cgacattcat ggcgtgaatg ctgagggtcg cggtgcattc aagcacctcc
    10681 atgatctcct gcggggtcgc gccggccgcc agggccgcct tgatgtgctg tcgggtgccc
    10741 ggcccgtaca ggtgcgtcac cgccgagtcg atggcgatgt agaggaactc cttcgtcttc
    10801 gtgtcgaggt ggttcttacg ccacggcacc gccgagaagt tcaggtaggc gtgaagaaac
    10861 tccgcatcga gggtgaggat cgcctcccac gtctcacccc aggttccgcg gacccgaatg
    10921 aactcgtctt tgacttcttg ctgcctcgcg gtgagtgcca tgacctttcc cttcttatcg
    10981 ttcgtcattc gtccgatgtc ttggctgcgc cggccagcgc gacgctctgc tcatggtcga
    11041 gcgcacgcat cgcggttctg ctggcccgat gcacgtggtc ggcgaacaag cgtgccgccg
    11101 ccgccccgtc gcggcgcccg atcgcctcaa ccgtggcgcg caattccgca acgacctcct
    11161 cggcccgccc ttcttgcccc agcgacgtcg cgcggagcat atggacccgg gccttcaggc
    11221 ccgccaagag ctgctccaga gtggaacttt gcgcaccttc gagcagcacg gcgtagaacc
    11281 cgtctttcgc cgccagaacc tcttgcacgt cgctgccgtc gtgaacggtg cgttcgaact
    11341 cgtcgacgct ggccgacagg cgggcgaccc ccgcctcgtc gacccgttcg acgaaatgcc
    11401 gcacgagcag agactccaac acggcacgga tctcgtacag gtcggccgct tcttctgcgg
    11461 aggggatgct gacgatggcg ccgcgctgcg gcatcaccgt gacaaggccg tcggagatca
    11521 actcacgcaa cgcctcgcgc acggtggtgc gagagacgcc gaggcgttcg atcaactccc
    11581 gctccacgag ccgttggcct ggtttcagat cgaagctgaa gatcgcctga cgtagcgacg
    11641 tgacgacttg ttcacgaagc ggcgcagcga cccggccgac cggcgtgatg gaccaggcgt
    11701 cgtctgtggc ctcgacaggc ggcatggcgc cctcctcgtt cgttcggccg ggttgatggt
    11761 ggctcacccg ggcttgatgt cgtcggcccg ctcttggatc cagcgcgcga cctccgtgtg
    11821 atcggcggcg ggttccaggc cctcggcggc cgcgctccac aaggcgatgg cgtcggcgcc
    11881 gagcctcgtg ggtgcgccga gctgctcggc cagcccggcc gcgatcttca tgtctttgag
    11941 catgaggcgc agcccgaagc ccgacgcata ggtcttcggc aagatgtagt tcggccactt
    12001 gttgtcggtg gagccgctgc gcccgctcga actgttgaag acggagagca tcacggccgg
    12061 gtcgattccg aagcgctggc ctgcgaccat cgcctcgctc gtcacccaga ggtgtgtggc
    12121 ggagagcagg ttgttgagcg ccttgattgc gtgccccgac ccgacggagc ccacgcgcac
    12181 gggccgccca agaggctcga cgacggtcgt gacgcggtcg aaaagctccg gcgggcaacc
    12241 gaccatgatc gtcagcgtgc ctttcgtcgc cccgctgacc ccgcccgaca ccggcgcgtc
    12301 gatgtagtcg atgcccttcg cgtggaatct ctcggccagt gctcgggtgc gcatcggctc
    12361 cgatgagctc atgtcgatga cgagcgtctc cgtcgtgagt gcggcagaga aaccggcgtc
    12421 gccggccacc gcctcgacga tgtcggagtt cgggagcatc aggatgacga cggacgcccc
    12481 ggccaccgcg tcggcgagac tggaggcagg caccgcgcca agctccgccg cgtgcgcgcg
    12541 agcctttcca ctcacgtcgt aggcctgaac acggtggccg gcctgcacga ggcgtgcggt
    12601 catgggcgcg cccatgttgc cgagaccgac gaaaccgacc gtagccgggg cgtcagccat
    12661 tctcgtcgat ctccttcatg accttctcgg cgatacggaa cgattcgagg gccgcaggca
    12721 cgccgacgta gatcgcggtc tgcatgagca cggcctggat ctcggcgacg gtgacgccgt
    12781 tgttcagcgc cccgcgcacg tgcgcgccca actcgtgacc ccggttcagc gcggtgagca
    12841 tcgcgaggtt gataaggctg cgggtgcgcc tgggcagctc gtcgccgctc cagatctcgc
    12901 cccagcagta ttcgctgacg agctcctgaa tcggccgcga gaagtcgctg accttcgcca
    12961 tggaacgatc gacgtgagcg tcgccgagca cggcccggcg cgtcgcgatc cctcgctcga
    13021 gagtctctcg gtgcgtcatg tctttgtcgg tcatcactct ttccttgctt ccggatcttg
    13081 atagtcggcg tcgtcaacct cgtcggccca gctcgtcacg cccagggaaa tggccgtatg
    13141 caccatgtat gcgtcgggcg ccgcgccgtg ccaatgcttc tcaccgggcg ggcaccagac
    13201 ggtgtcgccg gcccgaacca cctcgacggg gccgccctca ctctgcacga ggccgcggcc
    13261 tgcgagcacc tgcaagatct gaccgcgctc gtgcgcgtgc cagaacgtgt gcgcccccgg
    13321 cgtgaagttg accgtgttga cgaccgcccc ctcgccggca ggcttcgtca cgtagggata
    13381 ggcctttccc gtcacattgg agcggcggtg ccccgccaca ccctcttggc cttctgggtg
    13441 gatgacgatc atcgtgctgc ctcctggctg gtgccctcgt ggagtcgtac gccgcccgag
    13501 acatccccga tggcgtcgac aacggtgttg ctgatctcgt cggcgtaacc gagcgcccgg
    13561 gcgagcccga aggccgcgac aggtcccgac gagttgaggc tggcaacgcc caattgggcc
    13621 gccagctcgg tgtagagcgt gatgtctttg agcatcagcg agttggtcag gccacccttg
    13681 agatagtcgc cgtgaatgat cttgggaaag cggttcgtgg tggcgaagtt cacgcccgaa
    13741 ctggtgttca gcaccttgag aacggtctcg agatcgagat tcgccttctt cgccgccacc
    13801 atgacctcgg cggtggcgga gagtgatacc gcattcaaga agttgttgag cagtttcgtg
    13861 atgtggccgg tgccgctgac accgatatag aagatgttcg acgcgaagta acccaattcg
    13921 gcgcgcaccg agtcgagcac cgtcgcgttg ccgccgacca tcagggtgag gtctgccttc
    13981 tctgctcgcg ccgcgccgcc cgagatcccc gcgtcgaggt agttggcccc cttctcgaag
    14041 agagcgtcgt gaatctttct cgtcgagctg ggtgccgccg tgctgaggtc gacgatcacc
    14101 tggccggcgt gcgcgtgggc gacgaggcca ccatcgccgt acgcgatcga ctcgatgatg
    14161 tggctgttgg gcagtgacag gaagacgagg tcggtgttct cgaccacctc ggcgaccgac
    14221 gcggcaggcg acgcgccgac ggccgcgatg ttctcggtct tcgtgtcgaa gcccagcacc
    14281 tcttggccgg cacgcacgat gcatccggtc atccggccgc ccatgttgcc cagcccgatg
    14341 aatccgattc tcttctcagt catgctgcat ccctcatttc gatccgtaga tgacccggtc
    14401 ggcggcgacc ggatcccaag cccccgcgcc cgcagccggc cgcaccgtgt tgacgatggt
    14461 ggccccgccg tcgaccgaga cgatctggcc cgccatatac gacgcatccg agctcagcaa
    14521 gaacgccacg acgccggcga tctcgtcggg ggtgcccgct ctgcgcatcg gcgtagtgga
    14581 ggcgcggcgt tccatgtcgt tcttgccgcc ggtcgtggtg gccgcgtcgg cgaacagttc
    14641 ggtgggcacg atgccggggg cgaccgcgtt gacacggatg cccagcgggc cgccgtaggc
    14701 gcccgccccg tggacgaggc cgtgcacagc gtgcttcgac gtggtgtagg gcaagaggtc
    14761 ggcactgccg gtgattcctg cgatcgaccc ggtgacgacg atcgacccgg tgctggactg
    14821 cgccgcgaac tgccggaacg ctgcccgcag accgaggaac tgcccacgta cgttcacggc
    14881 gacgacgcga tcgaactcgg cgacgtcgat gtcgggcagg gcggcgaacg tgccgaagat
    14941 gcccgcgttc aggtggtaaa ggtcgatgtg gccgaagcgc tccaccccgg ccgccatgta
    15001 tcgttcgacg gcggcctcgt cggagatgtc ggcctcgacg gcgagagacg gcgtaggcag
    15061 gccttcgacc accgcatcga ccgcgcgggc gttgacgtcg accgcgacga cgttggcgcc
    15121 ttcggcggcc agacgccggg cagacgcgcg gccgaggccg ctgccggcgc cggtgacgac
    15181 ggcgactttg cctctgaagc gttcgctcat gaccgtgcct ccctggtcgt gaccggtgtg
    15241 ccgatcgtgg ccgcgacgcc ggcgagcgcg cctgcagcgg gtgtgaaccg gccgatggtg
    15301 tcggggtcgt ggccggccac gacgtgcttc acctcacccg agtcgagcat ggcgcggatg
    15361 cgggcgaagg tgtcatacat ctcgacgagg tcggcgacgg aactgaacgg catgtcgttc
    15421 gtcagctcct cgtaatagtg cacggcgtcg ctggcgagga ggacggtgcc ttgcgtcgtc
    15481 tgaaccttga cgacgctctg gcccggcgtg tgtcccccca cctcgatgac ctcgacgccg
    15541 ggggcaggcc tggtgctgcc gtcgaagaag tccacacgac cggcctcgac aaggccggcc
    15601 aaagtcgtga gctcgtcgtc ttcgaccgaa tggtggaaca ggggctggtt tgcgttcggc
    15661 cccgtccaga actcgtattc cttgcgcgag agcacgaacc gggccgccgg gaaggcatcg
    15721 agattgccga tgtggtcgta gtgggcgtgg gtgatgatga cggtctcgac ccgcgcggga
    15781 tcgacgccca accggcgcaa caactccggc acctcgacaa gaacgtctct tttacgcacc
    15841 tgcgcaccgt gccgcgagta cccggtgtcg accacgatcg tgcgctctgc actctccagc
    15901 acccaaacga agtagtccat gcccagcggg gcatcgggct gccgatagat cgggtaattc
    15961 aagaacacgt cgctacgcgc ggtcacccgg gtgccgtgcc gggcgatcgt gacgagatag
    16021 tcgtcgtggc cggcggaggt cacgccagca ccgtagcaat ctcgccggcg gcaccggggg
    16081 ccgccgggaa gcggtcacgg acgcgggcgt cgtgccccgg cacgaccgtc gcacccgtcg
    16141 tgcgggcgag ctcgttgatg aagctcagac cacgcttcat ctcgtcgagg ctcgtgaatg
    16201 cgaagaaagg ccactcgtgt tcgatctgct catagaaatg ggcggcgtca acggtgagaa
    16261 tgagcccacc gccggcggtc tgcaccttcg tcagaagctc tccggggcag tgccccccga
    16321 tcgggtagac ggtgattccg gggaacacct cggtctcgtc ggtcacgagc cacagccgac
    16381 cctgcttctc ggcctcgagc acgggccgca gatcgtcctc ggaggagaac tcgccttcga
    16441 gcgcgccggc atgcagcttg ccgatccaat agtcgtactc gacctgcgcg gacacaatct
    16501 gcgcattggc gaagagcttc agatagccga tgtggtcgta gtggtaatgg ctcgtgatga
    16561 ccatgctcac gtcggccggg tctatcccga tgatctgcag gccttcgggg gtgggggtga
    16621 cgctgatctc accgagccag tcacgcttgg gaatgtcgta tcccgtgtcg agcaggatga
    16681 tcttgccctg gctgcgtatg acccagaagt tgtagtccat gtcgaggttg ccgtcgggga
    16741 ggccgtattg ggcgtagtcg tggaagacat cgctcttacg cacgacccgc tcgccgtatt
    16801 tgaccgacca gaccgacacc gcgccatcgt gagattcggc tgtagtcacc atcgtgcaac
    16861 tcctttgttt gcagggcgcc gccggcccat cgccccgcca gaagcgaatc cggtctcaca
    16921 cccattgtgc ctgaccatat gatcatcata caagatttgt gctggcccag ccatacgtca
    16981 tcgacgtccg gagccgcggg gcacggccca ccgggcagga acagcgaccc cgacgcctcg
    17041 ccgcctactg agccaggtat ccgccatcga cgaccatctg cgtgccggtg acgtagctgg
    17101 agtcgtcgct ggccaagaac aagaccgccc gcgcgacctc gatcggctcg ccggcccgtt
    17161 tcatggccgt cttcgagatg accgcgtcac tgacgtcggc ggcctgcctc tccgtgagcg
    17221 gagtgttgat atggccgggg tgcacggaat tggctctgat gccgtcggcc gcatgggtaa
    17281 ccgcgacgtt cttggtcatg ttcgcgatcg cgcccttcgc cgcatgatag gcatgcgcac
    17341 ccgaaacggc cgccagcccc catatagagg agaaattcac gatcgacccc ccgccggagt
    17401 cgcgcatgag cggtataccc gcacgcatgc cgagaaacac ccccgtcatg tccaccgaca
    17461 tgacccggtt ccacgattcc agtgtcacgt ccaagatcgg ctcgtaggcg atcacgcccg
    17521 cattgttgac caagacgtcg agccgctgct gctcgccact cacccggtcg agaagacctt
    17581 gccacgagcc ttcgttcgcc acgtcgagat gaaccgggat aacaccgggg gccgcctcgc
    17641 cctcgaaagc gtttatgtcg cccgcgtaca ccgtggcgcc ctcttcggcg aacagccggg
    17701 tgatcgcacg gcccattccc tgtgccgcgc ccgtgacgat cgcgacctta cccagcaact
    17761 tctgtgccat ttctcctcca ctacctactc ggcccggccg ggccggcacc ttagaacgcc
    17821 actcaccacc cactcagttg tctcgtgacg tcgaccgcgc caatcggtgg gtgttaaagg
    17881 agtccagcac gatcgctacg aagatcaacg caccttgtac aaactgaacc cagaacgggc
    17941 tcacgttgat cagtgtgagc ccattgttaa tcacaccgat cagcagcacg ccaaagaatg
    18001 ttccgagcat gctacctttg ccaccgaaca ggctggtacc gccaatgacg acagctgcaa
    18061 taacctggag ctctaaccct gtgccgcccg tcggtgtcgc ggagcctaac cgccccagaa
    18121 ggatcatcgc cgaaatcccg ccgcagatcc ccgcga
//
LOCUS       DASTNL010000045         4397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_408081, whole genome shotgun sequence.
ACCESSION   DASTNL010000045 DASTNL010000000
VERSION     DASTNL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408081"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFQ96_01430"
     CDS             complement(<1..1241)
                     /locus_tag="VFQ96_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HEU0256482.1"
                     /translation="MRFYDATPTYDLTYSDVFLVPGHSDVTSRLDVSLAPEDGSGLTT
                     PIVSANMNSVTGRRLAATMARRGGIGVLPQDMPVEELAEAIRWVKEQPVAFDTPFFFD
                     EDQTVFDARQRLAPADGQGLVVRDAEGRLVGCVAATRLATALADARLGDLAQGPTATL
                     DAARIPDGRAAFDAMTEAEVDFAPVVSEGTVVGTMSLTSALRSTLYAPALDATGRLRV
                     AAAVGVNGDAAAKSRLLQAAGVDMIVVDTAHGDQDAMLRAVGQVARLDLGLPIVAGNV
                     VTAGAVRSLVAAGASIVKVGVGPGAMCTTRMMTAVGRPQFSAVLETAEAARAAGASVW
                     ADGGVRYPRDVALALAAGASAVMIGSWFAGTVEAPGVLRTDRDGRLYKESWGMASTKA
                     VQARFGRLGAYELARKTLFAEG"
     gene            1387..>4397
                     /locus_tag="VFQ96_01435"
     CDS             1387..>4397
                     /locus_tag="VFQ96_01435"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEU0256483.1"
                     /translation="MSSQLTGTGTNDGVTSRFGANEWLVEEMYEQYVADKNAVDPSWW
                     PILENYRSPLTAPSSPARRPAAAPSPAASAPVRPAVPASPSPARPATQPVARTTSVAP
                     KPQPVPADVPVSAPKAAGQEDSDEAGDTRPLRGMAKALAKNMDQSLAVPTATSARTIP
                     AKLMIDNRIVINNHLRRNRGGKVSFTHLIGWAMIQAIKTFPGQNVHYAEVDGKASIVS
                     PAHVGLGIAIDVKKADGSRALVVPSIKRADTMGFGEFLAAYEDLVSRARDGKLTADDY
                     AGTTISLTNPGGIGTVHSVPRLMAGQGCIIGAGALAYPAEFQGASTKTLTNMAIGKTI
                     TLTSTYDHRVIQGAASGEFLKILHGLLIGENRFYEDIFAALRIPYEPIRWAPDISVDL
                     ASAVDKTARVQELINSFRVRGHLMADVDPLEYRQRSHPDLDVATHGLTFWDLDRTFVA
                     GRLGGNDRTRMSLRDILGVLRDSYCRTTGIEYMHIDDPAERAWIQEHVEKPYAKPTHD
                     EQLHILGKLNQAEAFETFLQTKYVGQKRFSLEGGESTIALLDRILQGAADAGLDGVGI
                     GMAHRGRLNVLTNIAGKTYGQIFREFEGTTETGGDAGSGDVKYHLGTEGTFTAADGTQ
                     IPIYLAANPSHLEAVDAVLEGIVRAKQDHRPAGTFTTLPILIHGDAALAGQGVVSETL
                     QMSQLRPYRIGGTIHITINNQIGFTTSPNEGRTSVYSTDVAKAIQAPIFHVNGDDPEA
                     VVRVGELAFAFRQQFKKDVVIDLVCYRRRGHNEGDDPSMTQPLMYNLIGQKRSVRKLY
                     SEALIGRGDITEEEYDQAHQDFQDRLERAFAETHAAQTHSIPIVLPETQAGAQERQEP
                     ESTGVGEEVLHTIGDAFNNPPAGFTVHSKVARVLARRQEMSRNGKIDWAFGEMLALGS
                     LLLEGTPVRMAGQDSRRGTFVQRHAVMHDRENGQEWLPLANLSDNQARTWIYDSLLSE
                     YSAVAYEYGYSVERPDALVMWEAQFGDFMNGA"
BASE COUNT          776 a         1594 c         1431 g          596 t
ORIGIN      
        1 ccctccgcga agagggtctt gcgggccagc tcgtaggcgc cgagccggcc gaaacgcgcc
       61 tggacggcct tcgtgctggc catcccccag ctctccttgt agagccggcc gtcgcggtcg
      121 gtgcgcaaaa ccccgggcgc ctccaccgtg ccggcgaacc aggagccgat catgacggcc
      181 gaggcgccgg ccgcgagcgc gagcgcgaca tcgcgcgggt agcgaacgcc gccgtcggcc
      241 cagacgctcg caccggccgc gcgggcggcc tcggcagtct ccagcaccgc cgagaattgc
      301 gggcggccca ccgcggtcat catgcgggtc gtgcacatcg cgcccggccc caccccgacc
      361 ttgacgatgc tcgcgcccgc ggcgacgagc gagcgcaccg cgcccgccgt gacgacgttg
      421 ccggccacga tcggcaggcc caggtcgagt cgcgcgacct gccccaccgc gcgcaacatc
      481 gcgtcttggt cgccgtgcgc cgtgtcgacg acgatcatgt ccaccccggc cgcctggagc
      541 aaccgcgact tggcggcggc gtcgccgttc accccgacgg cggcggccac ccggagacgt
      601 cccgtcgcat cgagcgccgg cgcgtacagc gtcgagcgca gcgcgctcgt gaggctcatg
      661 gtgccgacca ccgtgccctc gctcaccacc ggggcgaagt cgacctccgc ctcggtcatc
      721 gcgtcgaacg cggcccggcc gtccgggatc cgtgccgcgt cgagcgtcgc ggtgggcccc
      781 tgcgcgaggt ctcccagccg ggcgtccgcc agggcggtcg cgagcctcgt ggccgcgacg
      841 cagccgacga gccgcccttc ggcgtcgcgc acgacgagcc cctgcccgtc ggccggcgcg
      901 aggcgctgcc tggcatcgaa cacggtctgg tcctcgtcga agaagaacgg cgtgtcgaag
      961 gcgaccggct gctccttgac ccaccggatc gcctccgcga gctcttcgac gggcatgtct
     1021 tgcgggagca ccccgatgcc gccccgccgt gccatggtcg cggcaagccg tcggccggtc
     1081 accgagttca tgttcgccga gacgatgggg gtggtcaggc cgctgccgtc ttccggcgcg
     1141 agcgagacgt cgagccggct cgtcacgtcg gaatgcccgg gcaccaggaa cacgtcagaa
     1201 tacgtcaagt cataggtcgg cgtggcgtcg tagaagcgca tgcgaacacg gtaccgtcac
     1261 ggctgcggga gggccggtcc cacgacttcc ggccaggccg atggattagg ctgatgccct
     1321 gaagtggggc ggcttcatgc ctcgccacac agcaactgga tcaacgggag agagtgggcg
     1381 atcggcttgt cgagccaatt gaccggtacc ggcaccaacg atggtgtaac cagcagattc
     1441 ggcgccaatg aatggctcgt cgaagagatg tatgaacaat acgtcgccga caagaacgcg
     1501 gtcgacccgt catggtggcc gatcctcgag aactaccgct ccccgttgac agccccgtcg
     1561 tcccccgccc gcaggccggc cgcggcgccc agccctgcgg cgtcggcccc ggtgaggccg
     1621 gccgttcctg cttccccgtc gccggcacgg ccggcgacgc agccggtcgc ccgcacgacc
     1681 tcggtggcgc cgaagccgca gcccgtgccc gccgacgtgc cggtcagcgc cccgaaggcc
     1741 gccgggcagg aggattcgga cgaggccgga gacacccggc cgctgcgggg catggcgaag
     1801 gcgctcgcca agaacatgga ccaaagcctc gcggtcccga ccgcgacgag cgcgcgcacc
     1861 ataccggcca agctgatgat cgacaaccgc atcgtcatca acaaccatct gcgccgcaat
     1921 cgcggcggca aggtctcgtt cacccacctc atcggctggg cgatgatcca ggcgatcaag
     1981 acgttccccg gccagaacgt gcactatgcc gaggtcgacg gcaaggcctc catcgtctcc
     2041 ccggcacatg tgggcctcgg catcgcgatc gacgtgaaga aggcagacgg gtcacgggcg
     2101 ctggtcgtgc cgagcatcaa acgggccgac acgatgggct tcggcgagtt cctcgccgcc
     2161 tacgaagacc tcgtctcccg ggcgcgagac ggcaagctca ccgccgacga ctacgcgggc
     2221 acgacgatct cgctgacgaa cccgggcggc atcggcacgg tccattcggt accccgcctc
     2281 atggcaggtc aaggttgcat catcggcgcg ggcgctctcg cctacccggc cgagttccaa
     2341 ggcgccagca ccaagaccct cacgaacatg gcgatcggca agacgatcac cctgacgagc
     2401 acctacgacc accgcgtcat ccagggcgct gccagcggcg agttcctgaa gatcctgcac
     2461 ggcctgctca tcggcgagaa ccgcttctac gaagacatct tcgccgcgct gcgcatcccc
     2521 tacgagccga tccgctgggc ccccgacatc agcgtcgacc tggccagcgc ggtggacaag
     2581 acagcgcggg tgcaagaact catcaactcc ttccgagtgc gcgggcactt gatggccgac
     2641 gtcgacccgc tcgaataccg ccagcgctcc caccccgacc tcgacgtcgc gacccacggc
     2701 ctcaccttct gggatctcga ccgcacgttc gtcgccggcc ggctgggtgg taacgaccgc
     2761 acacggatgt cgttgcgcga cattctcggc gtgctgcgcg actcgtactg ccgcacgacc
     2821 ggcatcgaat acatgcacat cgacgacccg gccgaacggg cctggatcca ggagcatgtc
     2881 gagaagccgt acgccaagcc gacccacgac gagcagctgc acattctcgg caagctcaac
     2941 caggcggagg cgttcgagac gttcctgcag acgaagtacg tcgggcagaa gcggttcagc
     3001 ctggagggcg gcgagtcgac gatcgcgctg ctcgaccgca tcctgcaagg cgcggcggat
     3061 gccggcctcg acggcgtcgg catcggcatg gcacaccgcg gccggctgaa cgtcttgacg
     3121 aacatcgcgg gcaagaccta cggccagatc ttccgtgagt tcgagggcac caccgagacg
     3181 ggcggcgacg ccggctccgg cgacgtcaaa taccatctgg gcacggaggg cacgttcacc
     3241 gccgccgacg gcacgcagat cccgatctac ctcgctgcga acccctccca cctcgaggcg
     3301 gtcgacgccg tcttggaagg tatcgtcagg gccaagcaag accaccggcc ggccggcacc
     3361 ttcacgacgc tgcccatcct catccacggg gacgcggcgc tcgccggcca gggcgtcgtc
     3421 agcgagacgt tgcagatgtc tcagctgcgg ccgtaccgca tcggcggcac catccacatc
     3481 acgatcaaca accagatcgg cttcacgacc tcgccgaacg agggacgcac gtccgtctac
     3541 tcgaccgacg tggccaaggc gatccaggcg cccatcttcc acgtcaacgg cgacgacccg
     3601 gaggcggtcg tgcgggtggg cgagttggcg ttcgccttcc ggcagcagtt caagaaagac
     3661 gtcgtcatcg acctggtctg ctaccgccgg cgcggccaca acgagggcga cgacccgtcg
     3721 atgacgcaac cgctcatgta caacctcatc ggccagaaac gcagcgtacg caagctgtac
     3781 agcgaggcgt tgatcggccg cggcgacatc accgaagaag aatacgacca ggcgcaccaa
     3841 gacttccaag accgcctcga gcgtgccttc gccgagaccc acgcggccca gacccactcg
     3901 atacccatcg tgctgcccga gacgcaggcc ggcgcgcagg aacggcaaga gccggagtcg
     3961 accggggtcg gcgaagaggt gctgcacacg atcggcgacg cgttcaacaa cccgcccgcc
     4021 gggttcacgg tgcactccaa ggtggcgagg gtgctggccc ggcggcagga gatgagccgg
     4081 aacggcaaga tcgactgggc cttcggcgag atgctcgccc tggggtccct gctcctggaa
     4141 ggcacccctg tgcgcatggc gggccaagac tctcgccgcg gcaccttcgt gcaacgccac
     4201 gcggtcatgc acgatcgaga gaacggccag gaatggctgc cgctcgcgaa cctcagcgac
     4261 aaccaggccc gcacctggat ctatgactcg cttctctcag aatattcggc ggtcgcctac
     4321 gagtatggct actcggtcga gaggccggac gcgctcgtca tgtgggaggc gcagttcggc
     4381 gacttcatga acggcgc
//
LOCUS       DASTNL010000046         4745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1519019, whole genome shotgun sequence.
ACCESSION   DASTNL010000046 DASTNL010000000
VERSION     DASTNL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519019"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFQ96_01440"
     CDS             complement(<1..321)
                     /locus_tag="VFQ96_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEU0256484.1"
                     /translation="MTRTETAAADEGFELDIGGMTCTACANTIERRLNRLDGVSATVN
                     FATERAIVAGRGREHAALAIAAVEKAGYTAAVHTPGDDTWSMRAAQMRTASLRRRLAV
                     SAVLA"
     gene            complement(336..2399)
                     /locus_tag="VFQ96_01445"
     CDS             complement(336..2399)
                     /locus_tag="VFQ96_01445"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU0256485.1"
                     /translation="MQTDPVSAEWLGLDQDPVRPAPPRPRMDLRRLWPLPAAAAPVAI
                     VVATVAILGPTPYRLLHDQEPGLATSMLSALLRLSNDASCAVTLGSLAVAAFVLARKS
                     RTGINLDRDLAVARVASIVWLVGSALSIGVDGADQEGLSLSKLFTPGALGYLITASYL
                     PGAWIASTCCAGVVFVASWLVHRWTSVAFLAAVTALAGLAPVMVGQVLVGPNHDFASD
                     ATIFATPAAAILFGAPVAMLARWHAVGPPGPQTIRRLRRLVFWCAGVAAAGDGITYWV
                     QSAGRPLTSATGLLFLPRIIVPLAAVAIFFVIPRRLRRGLRRFQLAGLAAACALALGV
                     TDAMVRIPPPVFFGPTDAQDQYLGYSVNAPLNPASLFLGARVNILFLVMAVAGVACYL
                     GWLRTLRRRGESWPAGRTVAWLLGWAGVVVTTSSGVGPYSAASYAVHMGLHMSLNMLC
                     PLLLVLGGPVTLALRALRTHAPSEHAGAREWLEALRHSRLNKIATNPMLILGVFVGSY
                     YAIYLTPFFGWAMRYHWAHQAMTVEYLEIGYAFFALVIGVDEAPRPLPHLGKLGLVLA
                     AMPFHAFFGVIVMTAKAPLAGLYFHYLDPVWLGNLMHDQQVAGAIAWSASEVPLLVVV
                     ILLVAQWTRQDARSAARAGVGIEASRADAAYDQMLVRLAERRAADPVAARAAPRPNEK
                     GEPPR"
     gene            2623..3624
                     /locus_tag="VFQ96_01450"
     CDS             2623..3624
                     /locus_tag="VFQ96_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0256486.1"
                     /translation="MAGSLRRVLSAAAVLAVPLLALAGCSAGDGQAAAQPAASSHPSA
                     GAQGRAVSVVDGTGAKVSLSRPATRVVALDPSSIEVLHDLGVPHIAYDSSEPLFVKSL
                     FGRTTGGGLTQIGGTWSKPDVEGIIAFRPDLVVGDAYPHAEIKPALKGVAPMYLVTRS
                     GGYRQAMKDFRNLGILAGRQAVADKDVASFTADLARARGASPDDQTSLIIWGQSASSF
                     QVPTVDDPSASVLAQVSKYPWGGSGAQGMSLSLEDILKVNPDVIFVESLARLANPHAP
                     SLSSQMAADPLWRQLKAVKDHRVYDVDPDVWHDDRGGLGLKEILDQAMPLLYPHTKR"
     gene            3621..4655
                     /locus_tag="VFQ96_01455"
     CDS             3621..4655
                     /locus_tag="VFQ96_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0256487.1"
                     /translation="MSAAAPARTTGIARPERGRPRPGLVAGVALAGLLVLAAADLVLG
                     IPDLGFGQVARALAGGGSPTVHALVVGSRLPRLVVGAEGGALLALAGLLLQDVMRNPL
                     AGPELLGVSSGSAAVMAVIITLALPVPPALQVWLALAGGALGGGVVVWGARFQRNALG
                     VILIGVAVSALLNAVITTLISLGSQANAALFYSYLVGTLQGRGWLQAGLIAPWLAVVA
                     AAFALAGRLSLLRVGETAAVGLGVRVGALRVTTLILAVAAVCAVVAVCGPIGYVALLA
                     PHVARRLVGTGDTRRVLPAALVIGAVLLTAADLLGRVVFTPLEVPAGVWTTLLGGPLL
                     ILVLGNRFER"
BASE COUNT          691 a         1645 c         1720 g          689 t
ORIGIN      
        1 cgccagcacg gccgacacgg cgaggcgccg gcgcagcgac gcggtgcgca tctgcgccgc
       61 ccgcatgctc cacgtgtcgt cgcccggggt gtgcacggcg gccgtgtagc cggccttctc
      121 cacggcggcg atcgccaggg ccgcgtgctc gcggccgcgg cctgcgacga tcgcgcgttc
      181 ggtcgcgaag ttgaccgtgg cgctcacgcc gtcgaggcgg ttcaggcggc gctcgatggt
      241 gttcgcgcag gccgtgcatg tcatgccgcc gatgtcgagc tcgaagcctt cgtcggctgc
      301 cgcggtctcg gtgcgtgtca tgggactgga tgtcgtcatc gcggcggctc ccccttctcg
      361 ttcggtcgag gggccgcccg ggcggccacc gggtcggcgg ctcgacgttc ggcgagtctt
      421 acgagcatct gatcgtatgc ggcgtcggcg cgcgaggcct cgatgccgac accggcccgg
      481 gcggcagagc gcgcatcctg tctcgtccac tgtgccacga gaaggatcac gacgacgaga
      541 agcggcacct cgctggcgct ccaggcgatc gcgccggcga cctgctggtc gtgcatgaga
      601 ttgccgagcc acaccgggtc gaggtaatgg aagtacaggc cggccagggg agccttggct
      661 gtcatgacga tcacgccgaa gaaggcgtgg aacggcatcg ccgccaacac caggccgagt
      721 ttgccgagat gaggcagcgg ccggggcgcc tcgtcgacgc cgatcacgag ggcgaagaac
      781 gcgtacccga tctccaggta ttcgacggtc atcgcctgat gtgcccagtg ataacgcatg
      841 gcccagccga agaacggggt caggtagatc gcgtagtacg agccgacgaa gacgccgagg
      901 atcagcatcg ggttggtcgc gatcttgttc aatcgtgagt gccgcagggc ctcgagccat
      961 tcacgcgccc ccgcgtgctc cgatggcgca tgggtgcgaa gcgcccgcaa ggcgagggtc
     1021 accgggccgc ccaagacgag aagcagcggg cagagcatgt tgaggctcat gtgcagcccc
     1081 atgtgcaccg cgtacgaggc cgccgaatag gggccgaccc cgctggatgt cgtcacgacg
     1141 acacccgccc agccgaggag ccatgccacg gtgcgccccg ccggccagct ctcacctcgg
     1201 cgacgcaacg tgcgcaacca gccgagatag cacgcgacgc ccgcgacggc catcacgaga
     1261 aagaggatgt tcacgcgcgc gccgagaaag aggctcgcag gattcagcgg ggcgttgacg
     1321 ctgtagccga ggtactggtc ttgcgcgtcg gtcggcccga agaacaccgg cggcgggatg
     1381 cgcaccatcg catccgtcac cccgagtgcc agggcgcacg cggccgcgag gccggcaagc
     1441 tggaagcgcc gaaggcctcg ccgcagccgc cgcggtatga cgaaaaatat cgccacggcg
     1501 gcgagcggca cgatgatccg gggcagaaag agcaggccgg tggcgctcgt cagcgggcgg
     1561 cccgccgact gcacccagta cgtgatgccg tcgccggcgg ccgccacgcc cgcgcaccag
     1621 aagacgaggc gccgaagacg ccggatcgtc tgcggccccg gcgggccgac cgcgtgccag
     1681 cgtgcgagca tcgccacggg cgcgccaaaa aggatcgcgg ccgccggggt cgcgaagatc
     1741 gtcgcgtcgg aggcgaagtc gtggttcggg ccgaccaaaa cctggccgac catgacgggg
     1801 gcgaggcccg cgagcgcggt gacggccgcg aggaaggcca cggaggtcca gcggtgcacc
     1861 agccacgagg cgacgaagac gacgcctgca cagcaggtcg aggcgatcca ggcgcccggc
     1921 aggtagcttg ccgtgatcag gtagccgagg gcgcctggcg tgaagagctt gctgagggag
     1981 agcccctcct ggtcggcgcc gtcgacgccg atcgacaggg ccgagccgac gagccagacg
     2041 atggaggcga cgcgtgcgac ggcgaggtcg cggtcgaggt tgatgccggt gcgcgacttt
     2101 ctggccaaga cgaaggccgc gacggcgagg ctgccgaggg tcaccgcgca cgaggcgtcg
     2161 ttggagaggc gcaagagggc gctcagcatc gaggtggcga ggccgggctc ctggtcgtgc
     2221 agcagccggt agggcgtggg gccgaggatc gcgacggtgg cgacgacgat cgccacgggc
     2281 gccgccgcgg ccggcagcgg ccacagccgc cgcagatcca tgcgggggcg gggcggggcg
     2341 gggcgcacgg ggtcctgatc gaggccgagc cattcggcgc tgaccggatc cgtctgcacc
     2401 tgaccctccg attcccgatc gacgccacga ctctacagct tgtcgagtgt acgggcggct
     2461 gctatcggcc ggatgagagt gtgaagatcg gtggccttcg cctgtgttcc catggatgcg
     2521 ccgcgaacat cgtgccgtct gctctactag ttgtagagtc gtctcgcggg cgttcgggcc
     2581 ggatccggcc ctgcgagagc gaaggatcgg gtgaaataac ggatggctgg atcgttacgg
     2641 cgggttctga gcgctgcggc ggttctggcc gtgcctcttc tcgccctggc cggctgctcc
     2701 gccggcgacg ggcaggccgc cgcgcagcct gcggcctcct cgcacccttc cgccggcgcc
     2761 cagggccggg cggtctccgt cgtcgacgga accggcgcga aggtgagcct ttcccggccg
     2821 gccacacgcg tggtggccct agacccgtcg tcgatcgagg tgcttcacga cctcggcgtg
     2881 ccacacatcg cctacgactc cagcgagccg ctcttcgtga aaagcctctt cgggcgcacg
     2941 acaggcgggg gcctcacgca gatcggcgga acctggagca aacccgacgt cgaaggcatc
     3001 atcgccttcc gccccgacct cgtcgtcggc gacgcttatc cgcacgccga gatcaagccg
     3061 gcgttgaagg gcgtggcgcc gatgtacctc gtcacccgtt ccggcggcta ccggcaggcg
     3121 atgaaagact tccgcaacct cggcatcctg gccggccgtc aggcggtcgc cgacaaggac
     3181 gtcgcctcgt tcacggctga cctggcccgt gcgcgggggg cctcgcccga cgatcagacc
     3241 tcgctcatta tctgggggca gtcagcatcg agcttccagg tgccgactgt cgacgacccc
     3301 tccgcctccg tgctcgccca ggtcagcaaa tacccgtggg gcggcagcgg ggcgcaaggc
     3361 atgtcgctct cgctggaaga catcctcaag gtcaaccccg acgtcatctt cgtcgagtcg
     3421 cttgcccggc tggccaaccc gcacgcgccg tcgctgtcct ctcagatggc ggccgatccg
     3481 ctgtggcggc agttgaaagc ggtgaaggac caccgcgtgt acgacgtcga tccggatgtc
     3541 tggcacgacg accgtggcgg gctcggcctg aaggaaatcc tcgaccaggc gatgccgctc
     3601 ctgtatccgc acacgaagcg gtgagcgccg ccgccccggc ccgcacgacg ggcatcgccc
     3661 ggccggagcg aggcaggccg cggccggggc tcgtcgcagg cgtcgcgctc gcgggtctcc
     3721 tcgtcctcgc cgcggcggat ctcgtgctcg gcattcccga cctggggttc ggccaggtgg
     3781 cgcgggccct ggccggcggc ggcagcccga ccgtgcacgc cctcgtcgtc ggctcacggc
     3841 tgccgcgact cgtcgtgggg gccgagggcg gggcgctcct cgccctggcc gggctgcttc
     3901 tgcaagacgt catgcgcaac ccgcttgccg gccccgaact gctcggcgtc tcctccggct
     3961 ccgcggccgt catggccgtc atcatcacgc tcgcgctgcc cgtgccgcct gcgctgcagg
     4021 tctggctcgc gctcgcaggc ggcgcgctcg gcggcggcgt cgtcgtctgg ggcgcgcgtt
     4081 tccaacgcaa cgccctcggc gtcatactca tcggtgtcgc cgtctcggcg ctcctgaacg
     4141 ccgtcatcac gacgctcatc agcctcggca gccaggcgaa tgccgcgctc ttctacagct
     4201 acctcgtcgg cacgctacaa ggccgcggct ggcttcaagc cgggctcatc gcaccgtggc
     4261 tggcggtcgt cgcggccgcg ttcgcgcttg ccggcaggct cagcctgctg agggtcggcg
     4321 agactgcggc cgtcggcctc ggcgtgcggg tcggagcgct gcgcgtcacg accttgatcc
     4381 tcgcggtcgc ggcggtgtgc gcggtcgtgg ccgtctgcgg gccgatcggc tacgtcgcgt
     4441 tgctcgcacc ccacgtcgcc aggcgcctgg tcggcacggg agacacccga cgggtgttgc
     4501 ccgcggcgct cgtgattggc gccgtgctgc tcaccgccgc cgacctcttg gggcgcgtcg
     4561 tcttcacgcc gttggaggtg ccggcgggcg tatggacgac gctcctcggc gggccgctcc
     4621 tgatcctcgt gctcggcaac aggttcgaac ggtgacgcat acaggcggtt cggggcagcg
     4681 ccggggtgcc gtggcccggc gtcgccccgg cgtggcgggc gccccggaaa tcctgcgccg
     4741 tgccg
//
LOCUS       DASTNL010000047        32320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_593453, whole genome shotgun sequence.
ACCESSION   DASTNL010000047 DASTNL010000000
VERSION     DASTNL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 32320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32320
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593453"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..979
                     /locus_tag="VFQ96_01460"
     CDS             38..979
                     /locus_tag="VFQ96_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0256488.1"
                     /translation="MTTSVPGEISTDRVASAAPRAVSAGLTAPRPEGRPVTVAGGAGA
                     APAATEKTPLLGGDVPLARMPAHWMLGRLGKKVMRPGGLTPSLHMLGALQVGERDDVV
                     DLWPGLGVTTQRTLAAHPHSYLAIERGPAEAARVRRLLTRDEEQVVVGPVHKTGLADG
                     AASVLYGEALLTLEPARRKTAIVAEAARLLRPGGRYGIHELLLTPDTLSEAAKDDIER
                     TLTKVLRVGARPLTVSEWRRLVEEGGFEVQTEETGKMLLLDIPTFLSDEGVWGSTLFT
                     ARCLAHPSVIPRITQMWNVFRRYRDNLGAIVMTARRA"
     gene            1054..1482
                     /locus_tag="VFQ96_01465"
     CDS             1054..1482
                     /locus_tag="VFQ96_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU0256489.1"
                     /translation="MPDSHAPGRAHRHTAQKAAIRAALEHSDSFVSAQDLHAALRDDG
                     VTLGLATVYRNLNEMAESGEADVLQAEPNVQLYRFCGEEHHHHLYCVSCGRTIEIEAP
                     IEEWVDAVTAEHGFTRVRHVVDVFGVCADCRASGRGDEAH"
     gene            1978..2925
                     /locus_tag="VFQ96_01470"
     CDS             1978..2925
                     /locus_tag="VFQ96_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEU0256490.1"
                     /translation="MRIGKMMAAGLAAIAVVAGLAGCAPAEGLVGRMGAGAGGGADRI
                     LVVGAENQYSDVAAQIGGKYVTATAIIQNPNTDPHTFEASVSVAKDLSAARIVVQNGL
                     GYDDFMTKLESASPSKDRIVITAQDVLGLPNDTANPHLWYDPKTMPAVAKRIAASLGR
                     IDPSHKAYFAKNLATFDASMKTWGDQLAAFRSTHAGTPVAVTEPVADDLLKAAGVDVE
                     TPWSLQAAIMNDTDPSPQDSALQNALLTGKKVKALLYNRQVTDQVTERFLRVARANGI
                     PVVGVSETMPAGYHYQGWMEATLKALRVAIETGASTDKR"
     gene            2975..3886
                     /locus_tag="VFQ96_01475"
     CDS             2975..3886
                     /locus_tag="VFQ96_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU0256491.1"
                     /translation="MKARRAMVMPGGAGTAEAREPAAGAVLVARDIHVALAGRSILDG
                     VDLTVGAGEFVGLIGANGAGKTTLLRVLLGVLSPGSGEVMRPDGAGLLTDARHGRGVG
                     YLPQKVALDPEAPLRARDVVALGLDGGRPGLRLRGRAAMRERVDETLRAVGAAEFGDR
                     RVGELSGGQQQRVLLAHALISDPALLLLDEPLANLDPASAHDIVALLDRLRHTRPVAI
                     VMTAHDMNLLLPVMDRVVYLAGGRAATGAPAEVVRGDVLTRLYGRPISVIHAKGRVLV
                     IAGDDAAPADEVHHDTAFGSTGGQGRP"
     gene            3901..4800
                     /locus_tag="VFQ96_01480"
     CDS             3901..4800
                     /locus_tag="VFQ96_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU0256492.1"
                     /translation="MTGFLASPTVHTALIVGGFVAVITGIVGVFTVVRGQSFAGHALA
                     DLGAVGGSGAFLIGLSQVWGFVGAGIIAALLMESIGVRRLQGRDVATGVVFAFGMGVT
                     ALFLYWGTTVGGSSNAAVSVLFGSLFVIDPAVIPVVVGLSVLALVVVVVCYRWLLVES
                     VDRDLAAARGVPVRLTGVLFLVALALAVELSSLTIGAILSTGLLIGPAATALLVTRRF
                     GRAVLLSAGLALAITWAGCLVSYESYYWTGRQGNWPVSFCIVALIFLVYLGVRLGAAL
                     GRRHAAAHAGARPAAPRPLKAGV"
     gene            4800..5618
                     /locus_tag="VFQ96_01485"
     CDS             4800..5618
                     /locus_tag="VFQ96_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU0256493.1"
                     /translation="MFAPFMTNAWVAGTIIAVVAGVVGFFVVARGNSFLAHAIPHGAF
                     AGAAGAVLVGIDPLLGLGLFAAGGALTISFLGRRARSDVVTALPLVMMLGLGVFFLSR
                     SNQYSAEVFALLFGQVLGVSGAEIVPILLLGLVCLATVALLYRPLLLASVLPDMAAPR
                     GVSTQAVSVMFGLILACATTMSVPVVGALLMFALLVGPPAAAGFFVSRPGRAIALAVA
                     FALVTVWVSVALAYATDWPVGFFVTAVSALLYFVGRLFAVLRGRRSFAMRDALP"
     gene            5781..5853
                     /locus_tag="VFQ96_01490"
     tRNA            5781..5853
                     /locus_tag="VFQ96_01490"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5814..5816,aa:Trp,seq:cca)
     gene            5897..6169
                     /gene="secE"
                     /locus_tag="VFQ96_01495"
     CDS             5897..6169
                     /gene="secE"
                     /locus_tag="VFQ96_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU0256494.1"
                     /translation="MARRAVDEPGEDVVARRTRGDAAASQGFFARIIRFFHEVMAELR
                     KVTTPTRSELVNFTIVVLAFVIVMVGIVALFDWIFGMVVVWAFGGS"
     gene            6231..7016
                     /gene="nusG"
                     /locus_tag="VFQ96_01500"
     CDS             6231..7016
                     /gene="nusG"
                     /locus_tag="VFQ96_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU0256495.1"
                     /translation="MSETEQDGAEATATAPVTTSREAPAGADPVERGLDAALDAVAQA
                     QGSEPDADAADDEADPYEEFRTELRMKPGKWYVIHSYAGFERRVKSNIEGRMVSMEME
                     DYVYQVEVPMEDVVEIKNGQRKMVTRVRVPGYVLVRMELNEDSWSMVRHTPGVTGFVG
                     NAHNPTPLRFNEAFEMLKSLVQVQPTAKAGAVGGRGGAARTLAAEVDFEIGETITIKD
                     GSFAGLPGTISEIKPESGKLTVLVSLFERETPVELSFDQVTKL"
     gene            7119..7547
                     /gene="rplK"
                     /locus_tag="VFQ96_01505"
     CDS             7119..7547
                     /gene="rplK"
                     /locus_tag="VFQ96_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU0256496.1"
                     /translation="MAPKKKVSGLIKLQIQAGAANPAPPIGPALGQHGVNIMEFCKAY
                     NAATESQRGNVIPVEITVYEDRSFTFVLKTPPAAELIKKAAGVPKGSGTPHSSKVGKI
                     TLDQVRSIAEQKMSDLNANDIDAASKIIAGTARSMGITVE"
     gene            7565..8254
                     /gene="rplA"
                     /locus_tag="VFQ96_01510"
     CDS             7565..8254
                     /gene="rplA"
                     /locus_tag="VFQ96_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU0256497.1"
                     /translation="MAKKSKAYRAAASKIAADTYYAPGDAVALARETGSARFNSTVEV
                     ALKLGVDPRKADQMVRGTVILPHGTGKTARVIVFATGAAAEAAIAAGADEVGDDDLIA
                     KVQGGWTDFDSAVAVPALMGKVGRLGKILGPRGLMPNPKTGTVTPDPAKAVTEIKGGK
                     IEFRVDKHANVHFIVGKASFSSDQLSENITAALDEIVRLRPSTTKGRYIQKGTVSTTF
                     GPGIPLDVNAI"
     gene            8522..9889
                     /locus_tag="VFQ96_01515"
     CDS             8522..9889
                     /locus_tag="VFQ96_01515"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0256498.1"
                     /translation="MARWTLRGRLRPRIWIIAGTIVVVLAGAAVYWFGFGMPHAAPAE
                     AATRTVQAATQTLEETVSGTGSLQPTVDEDVNFAASGTVTAVKVAAGNTVKKGQALAT
                     IDTLTVQANLLSAQASLDSAQASLSDDEAAGDATTAQQAKIAADKAALQQAEASVSAA
                     KKARAATTLKAPVAGLVTAANLQVGDAVTASSSSNANAAGASSSSSAGSTSSGQGSSS
                     SGFGGVGSGSSSSTSSSSAGSGAQFEIVGTTSWQTSVSLSAADVKQVAKGDQVELSTD
                     DNPSFFGTVSSIGLLPTVSSGAATYPVVVTVTGSPDDLYDGVSVTAKIVYKRIQNALV
                     VPSLAVTTRGGSTSVQKIVNGKQVTTPVTTGETSGEFTQITKGLKAGDQVALQIFTPT
                     AGRGSSDGGAPGGGQGGFPAGSFGGGQRGFSGGGGFPGGGNFSGGGAGGFGGGQGGFG
                     GGNRG"
     gene            9882..10637
                     /locus_tag="VFQ96_01520"
     CDS             9882..10637
                     /locus_tag="VFQ96_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256499.1"
                     /translation="MAEAQTPVAGAATSGDPVIELVDAGKTYRSGSLEFEALRGIDLR
                     IEAGDYVAVVGPSGSGKSTIMNILGCLDVLSRGRYRLGGQDVEDLDEVELARIRNRRI
                     GFVFQQFNLLPSLSALHNVELPMVYAGVARPERVRRAREALERVGLGERLRNRPGELS
                     GGQQQRVAVARAIVTEPTIVLADEPTGNLDSVATRDILTLFDELHDAGRTIVLITHEA
                     DVAAHAERTIQVRDGRVLEADAADPMVGRAGAR"
     gene            10634..11866
                     /locus_tag="VFQ96_01525"
     CDS             10634..11866
                     /locus_tag="VFQ96_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256500.1"
                     /translation="MTWLETLHTAWTAVHTHTMRSILTALGVLIGIAAVILTVGLGLG
                     TQQNVTAQIDALGSNLLIVSPGSSTSTSGVRGGFGTGSTLTLADAEALQSKVNAPDVK
                     AVAPQKSTELSLTAGQENWTTSVVGVTPSWLAVRGRSLSEGSFITAKQNADQADVVVL
                     GTQTAEELFGTTRVVGQQVTISGQSLEVVGVLASAGSNSSSNLDDMAVVPISTAASQI
                     IGGTNQDSVSSVYVEATSQSTLSAADQETTNLLLNLHGIATSTDADFTVSSQDSLVST
                     ATAVYRTLTVLLTGIAALSLIVGGIGVMNIMLVSVTERTKEIGLRKALGAPPSAIRWQ
                     FLVEAAILGLSGGILGAALGIGSALALPGVIGSPIVVSAPAVAGSIAVAVAVGLVFGV
                     YPVARAARLAPIDALRSE"
     gene            11878..12885
                     /locus_tag="VFQ96_01530"
     CDS             11878..12885
                     /locus_tag="VFQ96_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256501.1"
                     /translation="MTFPTASITPAVPVKENPMSSSRSARCIFAVAVALAVPLALVGC
                     STGSHAKASPTTAAAQAPSASGGGAGGRGGQGFPGVSGEIAAIQGSTLQVQGSGQQTA
                     VTYTDSTAITQTVTGSLSDVSVGECVTGITEPAGQGSSASAKPGTAATTVTISKASSD
                     GTCTGGFGGGGFGGSGGRGGFSGARPSGFPTPRAGSSRPTFRPGAGGRSGFTPPVSGV
                     VTAVGSSTITVKETLPARGSSSSSASGSSSTKTITVGAKTKYQKNATATGSAVKVGLC
                     VVAGGKAGSNGTVAATSLRLSPKTSQGCTGFGGAGFGRGGFGGAGGAGAGQGGGSGSN
                     D"
     gene            12878..14830
                     /locus_tag="VFQ96_01535"
     CDS             12878..14830
                     /locus_tag="VFQ96_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0256502.1"
                     /translation="MTSAHKHRRHRRRRIVVGSALLVAVLLAAGGAAYAATRPQAPSY
                     RTAAAAMGAVHQTVDLDGQVASSNRRDVAFQTSGTVQAVDVSLGQAVTAGQKLAGLDS
                     SSLESALTDAQNAVTQAKQTLADDLASQTQTTEASSAAAGGRAGGASSGQGTSGGQAG
                     GASPFAGAGGSGTASSGASMGSHPSTGGQGTSADSAAVAAARKAVTTAQQALLAQVTA
                     AQKSLTATDTIVTTATTTCAPFLKAALGGDSSGGAGGTSSTAQQLTDCQGVIDQALAS
                     QKATAEAQSKVQSLAATLNTAVAKLQALLTGASTGASSGKTSSGGASPTPGSTGSPSA
                     GRDAPSVSLGRGAGATVELAADVSTSATSPQDGTAQDGSAQSGSGRSASSLSGSASQP
                     ASAATIVADQAAVAEKQAAVDVAKQALTFATLTSPIAGTVAAIGVAPGDSVTGGSTTE
                     VITVIGAGGNQVTSTATLTQVKELTVGQTVSVTADGRPLSGHVTSIGVQNVSTDTAAT
                     AYDVTIDLDGSSADLLNGTPAQAIVKVASLQHVLTVPTSAIHRNGTAYTVDVLQGGKA
                     VTKTVTVKVQGSIRTAITGVSTGAKVILADLDQQIAGTDSSSSSSSTSGLGGLSGLGG
                     STGGASRFGGVGGFPAGGFRGRTVGR"
     gene            complement(14923..15888)
                     /locus_tag="VFQ96_01540"
     CDS             complement(14923..15888)
                     /locus_tag="VFQ96_01540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0256503.1"
                     /translation="MLMRAAVIDRTGDPDVFHIAQVTAPHRISAEFLVRVHAAGINPI
                     DTKTRAGGGTSAAIAAFPAVLGHDFSGTVLESPDAAHPIRPGDEVFGMIMSPRFSGSF
                     GEIAAVPSLSVARKPATLGHVEAAAVPIAALTAWRCVTELASAAPGRRVLIHAAAGGV
                     GHFAVQFAHHLGAHVIATSSASHADFVRSLGADEVVDYRRQRFEEVIDPVDAVIDLIG
                     NVHDDTGTRSLGVLKPGGLIVNVPTGSWPSFFDDVARAGVRGTDVKVAPDARVLAKIG
                     ALIDEGTVRVHLEEVFNLADMGRAHELVETGHVAGKVAVRIAREE"
     gene            complement(16013..16603)
                     /locus_tag="VFQ96_01545"
     CDS             complement(16013..16603)
                     /locus_tag="VFQ96_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEU0256504.1"
                     /translation="MLAESLRRRVVADSGHRAAWLQARLRGVTATDAAHLATDRSVVQ
                     AAWHKLHPPTFTGNAYTEHGKAREPIVAEWARRRYGLNPSTLLFRSETSAEHLATPDG
                     MILRPDGRLELEEIKTTTKPWRHIPRPYLRQVWWQQYVLGAERTLVVWEEHRDFVPVE
                     PEPRCRWVDRDENEIEILVRRAERMLAIIHERTPAA"
     gene            16757..17524
                     /locus_tag="VFQ96_01550"
     CDS             16757..17524
                     /locus_tag="VFQ96_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0256505.1"
                     /translation="MSIKSVEDKAGSGHRTQAVLRRAGGGKVRVLVVDDENTLTDLLS
                     MALRYEDWEVHTADTGQKALAQAREFRPDLVVLDIMLPDIDGLTVLSRLRSDGFDAPV
                     LFLTAKDALDDRVAGLTAGGDDYVTKPFSLEELVARLRGLLRRSRIAVPETSDPVLVV
                     GDLTLDEDSYEVTRAGESISLTATEFELLRFLMRNPRRVLSKAQILDRVWSYDFGGSS
                     SVVELYISYLRKKIDAGRAPMIHTVRGAGYMVKAASA"
     gene            17521..19101
                     /locus_tag="VFQ96_01555"
     CDS             17521..19101
                     /locus_tag="VFQ96_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0256506.1"
                     /translation="MTGGARAAPETGASPRRPALLRRLAAAARLGAWSLRARLVVTAV
                     VMMALISVIIGVLSVLALRNSMMADLDSQLHSTIDRGRAVVLHEMQNFAAPGRPLIGA
                     VVAATGQPTGTFGAYVTDQSITVAYLDPAATTKNVTRNLSTTLTDIPADQGAQTVDLG
                     GGFGDYRLAATRVGPGVLVIGLPVAGVDQTISRLIVTIVVVSLLGLIVLAVAAAMIVR
                     LALSPLERMAAAARKVSELPLDRGEVALGVRMPQKDAQMHTEVGMVAAALNRMLGHIA
                     AALAARQRSEQKVREFVADASHELRTPLASIRGYAELTRRSPQELPADAGRALGRIES
                     EAARMTGLVEDLLLLARLDAGRDLEYGVVDLTSLVIDSVSDAHAAGPGHRFELDLPEE
                     PVEIYGDAARLHQVVVNLLANARVHTPDGTTVTAVVRREDGRAAGAPLAVPHAVVSVV
                     DDGPGIPEPQQARLFERFARGDASRSRATGSTGLGLAIVKAVVDAHHGLVKVSSVPGR
                     TEFTVRLPLDSPPPPPNA"
     gene            19344..19859
                     /gene="rplJ"
                     /locus_tag="VFQ96_01560"
     CDS             19344..19859
                     /gene="rplJ"
                     /locus_tag="VFQ96_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU0256507.1"
                     /translation="MADKHAAVAELTQKFRDSTAVLLTEYRGLTVTAMKNLRRSISAD
                     ATYAVVKNTLTKIAAHDAGITVFDDQLAGPSAIAFVHGDPVAAAKGLRDFANDNPLLV
                     IKGGYFDGHALNADEVNQLAELESRDVLLAKLAGAFKASLFGAAYLFNAPLSKAVRTV
                     DALREKQEEAA"
     gene            19914..20300
                     /gene="rplL"
                     /locus_tag="VFQ96_01565"
     CDS             19914..20300
                     /gene="rplL"
                     /locus_tag="VFQ96_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU0256508.1"
                     /translation="MAKLSTDELLDAFKELTLIELNEFVKKFEETFEVTAAAPVAVAA
                     PAAAGGAGEPAAEEKDSFDVILETVGDKKIQVIKEVRSLTSLGLGDAKALVDGAPKPV
                     LEGANKEDAEKAKAKLEAAGATVTLK"
     gene            20417..20956
                     /locus_tag="VFQ96_01570"
     CDS             20417..20956
                     /locus_tag="VFQ96_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0256509.1"
                     /translation="MIPPEPDARTARLQETISDLIQVSHRMARLAAHVSGSTESPASW
                     RTLSVLQAYGPMRLGELAERSRVSQPTMTKLVRGLVDRGRARRLADSDDARAWQIGIT
                     ADGDEALEGWRHTIGTALAPLFADITDEELRTLQEGVRIVSAGERRADGIDLLGSAKG
                     SATPPRGASPAAPAAGGAL"
     gene            21034..22206
                     /locus_tag="VFQ96_01575"
     CDS             21034..22206
                     /locus_tag="VFQ96_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256510.1"
                     /translation="MLHQPTAVWAVAFACVIAFMGIGLVDPILPAIAAELKASPTQTE
                     LLFTSYLIITAIAMFFSSWISSRIGAKRTLLIGLALIVLFAAAAGAAGNVEGVIGFRG
                     GWGLGNALFISTALAAIVGAASGGASSAIVLYEAALGLGIAVGPLLGGLLGGISWRGP
                     FFGTAVLMAVAFLSILILLKPGRARPAPSPLAAPFRALGRPGLTVMAVAALFYNFAFF
                     VLLAYTPFALVPLGLASAMTLGLIFCAWGVTLAISSVWVAPLFTRRMRRTRVMWLVLP
                     FFAADMVAAAFVIHSGAALIGCVIAGGLFLGILNTVMTQCSMESTDLPRMVASSAYSG
                     VRFLGGAIAPPLATILARLVDPATPFYVAAGALLVSLGITAVGVKALRRADAAPAS"
     gene            22279..24318
                     /locus_tag="VFQ96_01580"
     CDS             22279..24318
                     /locus_tag="VFQ96_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0256511.1"
                     /translation="MAAFASGWMRELVRPKSAPVPWARVFRCAVSITAPVALGAVVGR
                     LDIGLLVAIGGLTGALNDRDAPYLARMTRMAVVAVGGTVGFGLGGLVLGDEVLTAVFV
                     LAGGLVAALVSVLGGAASSAALQFLIYLIVASGVDFGPGPAWLGPACYLGGIAWALAL
                     SLVGGLGRSAAPERLAVAEVYRCLALVMESAGGPSAQADAARQKLTGAMNEAYDAVAT
                     RRTLSAGRDSHARLLAALVNAVTPAVETTTALVRSGTRVPVSFIARVREMSSEIRRGA
                     RGVPRPAGWVVSAQHPRAAWLRLLHDELDTVDEILEATRHARRPARPATTGGVTPVTR
                     ALKMAGMTTSVPVSGAGRQAIRSLRDAVASGPATWSPIVRLVLCLAVAEAVAILDPEP
                     RPYWIAMTVAIVLKPDFGSVFARAVQRGTGTIVGVLLGSVVLALAPGVVLPIVAIAIL
                     SALLPVAVGRNYGMYATFLTPLIVLLLDLGQGASGPGGAQLVVSRLVDTVLGCVIVLL
                     VGYVPWPDTWRSRGRLGARFATAVVAVRDYVTAALGAPADPGPSRQSARRTAYRALSD
                     LRTYLQQSLAEPPAVSAVAAGWWPAVVALERVTDAVTGVVVGARAGRARPGADGVRRV
                     VTALDVLAGRLRGEPAEAAGAATSSTVTDPALAGVWAELRTAQAVFGTRTPPPGP"
     gene            24331..25479
                     /locus_tag="VFQ96_01585"
     CDS             24331..25479
                     /locus_tag="VFQ96_01585"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU0256512.1"
                     /translation="MTSTGGFATRAIHAGQEPDRQTGAVVPPIYQTSTFVQDGINKLR
                     GGYEYSRSANPTRTMLESLLASLEGGARALSFASGLAAEDALLRTVLAPESRVVVSRD
                     AYGGTYRLIDRVHGAWGVRNTPADLTDLAAAREALAAAAVDLVWVETPSNPLLTVTDI
                     AALARLGHEAGALVVVDNTFASPALQNPLALGADVVVHSTTKYLGGHSDVLGGALVFA
                     DGDLAERVAAVQFSAGAVSSPMDAWLTVRGIKTLAVRIARHCANAQAVAEHLAGHPAI
                     ETVYYPGLDTHPGHALAARQMRGFGGMVSVAVRGGAGAAMRLVESTRLFQLAESLGGV
                     ESLICYPSEMTHAAVKGTPLAVADNVVRLSVGLEEADDLIADLDAALA"
     gene            complement(25476..26447)
                     /locus_tag="VFQ96_01590"
     CDS             complement(25476..26447)
                     /locus_tag="VFQ96_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU0256513.1"
                     /translation="MSADLSTPTRARLGRGAAGAPRSVRVAGWVSLVTEILIIGTGGA
                     VRLTGSGLGCKWPLCAPDSLVPVPGMSFHSYVEFGNRMMSGVVGLAALAVLLLVWRMR
                     KERRDLFTLAWIVIGGVLLQALMGGVTVLSHLNYDVVGSHFLISMILIIVAAVFVARA
                     PLAPGPRRWAAPRWIVGMTHTAAVLTALTLILGVVTSESGPHSGNADVRHHLFNAVVM
                     AHVHSGPAYALLAVSIVLVIGMARARLRPLGWSIGLLVLLLIQIPLGIYQARDGLPAV
                     AIESHMLLAALITATMAILVMRLRVPAAAPGPEAPTTQDSPAAAPVR"
     gene            26802..27338
                     /locus_tag="VFQ96_01595"
     CDS             26802..27338
                     /locus_tag="VFQ96_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEU0256514.1"
                     /translation="MNGVEALADGFGRISGVVHAVLDGISDEALTFRADPDANTVAWL
                     VWHLARSQDAQVAHLVAGDAGAGAADAAGAWQAEGFGERFALPFGPGETGYGHTSAKV
                     AAVRAPAGLLGEYYDAVAARTTAYVAGLADADLDRVIDTSWTPPVTVGVRLVSILADG
                     LQHAGQAAFVRGVAQRVR"
     gene            27379..28572
                     /locus_tag="VFQ96_01600"
     CDS             27379..28572
                     /locus_tag="VFQ96_01600"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU0256515.1"
                     /translation="MRVVIAPDSFKGTLGAAQVAASLAAGWREVRAADEVACLPMADG
                     GEGTLDAIRAARPDARRMPVAVQGPDGRTHAAEWLLLPDGGGVADDHDRNGDDRGGGI
                     GVIELAATSGLTLLDRLRPFDAHTVGFGQAIAAALRHGVRGLLLAIGGSSSTDGGTGM
                     LRALGARFQDAGGAPIPLGGGGLDRLVVADLDGLPALPPGGAEVLSDVTNPLCGPRGA
                     AFVYGEQKGADAAARSRLDAGLAHLAGVVGQARASDPGAGAAGGTGFGLLVWGDHAAK
                     RRDAGAEGEAAGRLVIVSGARRVARAIGLPQAVAGADVIVTGEGRFDGQSSEGKAPWL
                     VSGLPSPARRYLAAGSIEAPTPGFAAAVSLVDLASGLAAAKAEPERWLQRAGAELARL
                     AQREN"
     gene            complement(28524..30101)
                     /locus_tag="VFQ96_01605"
     CDS             complement(28524..30101)
                     /locus_tag="VFQ96_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU0256516.1"
                     /translation="MPAVTDGAAPVRVHRITPRAHGAGPSGVRTTRTLVAGIDSAAGG
                     CTVVVRDAATGERVRSGRAAAPAGAEADPHAWWQALLDAIEASGGFADVSAFGIGAAD
                     GQAVTLDAEGHLVRAALVGDERGFARAALDLVAEVGRQALAARVGSIPAGSHPLAKLR
                     WLRDGEPENAGRVEAVALRHDWLMWRLRGFGPRDEMPNGLRLEELVTDRSGASGTGYW
                     NPATGKWDHDLFGQALGRPAIKPRVMPPDAWGGESWKMPSHGIKSERVISIGGTRPAI
                     AALGLGLAPGDIMVSLGSGAVSTMAPAPVVDETGAIRSLADAAGGFLPTVVTPGDASA
                     LAGVARLLGVTNDGLARLAGAARPGAGGVVFTPRTAEFHAPDATAKTPENLARAALEG
                     VLCTLAAAADALATAGLPARRVIVIGPGAGRGVVQQATAQVFGLPVAVPQAAGPSSAA
                     SSPTAVRAAGGAASGPAAARSIVADGAAAQAVWAKTHRRASWHTPLTPEREADPRPEV
                     RDQFSRWASRASSAPAR"
     gene            complement(30167..31912)
                     /locus_tag="VFQ96_01610"
     CDS             complement(30167..31912)
                     /locus_tag="VFQ96_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0256517.1"
                     /translation="MSTAHHPRSTVAHHTDAGGVKLKRSVGPVGLLFAAVGSIIGSGW
                     LFGAYQASAIAGPAAIFSWLIAAVMIILIGLCYAELGPMLPISGGVVRYPHLVWGSFG
                     SYSLGFITWIAAAAVPAIEVTGALTYMTKFAPFTTKATSGSTTAQILTPLGIVVAVLL
                     LALFVVVNYFGVKLFAEINTVVVWWKLFVVCLVIVVFLITAFGTTRMGSPANFSQFGF
                     APTGFAGVFICISTAGITFSYLGFRQGIELAGETSNPRRNIPLALIGSVAITAVLYAL
                     IQVVYTLAVPHSVLVKSHGWDGLQFSGDFGPLAALAGMAGLAWLAYVLYFDAFISPAD
                     TGFIYTTVTSRLSYAMGRNGNSPRFLAKNNKYAVPHWSLLVTFLVGVVLLAPFPSWQS
                     LVGFITSATVMSFGSGPLVVAVLRRTHPELKRPFKLPGGDIIPYLAFFSANMIMFWAG
                     WSTNFKVIITILIGYAVLVLFQLFGHKEGRPPLLFKVGAAWIIPWFALYGLVSFFFSD
                     SAVFGTAWVQHSFFWVFLVNAVVSAFIYWLALKITLPKRLIDQYVADAARESASDDAA
                     SDDAGQSSGSTAHRA"
BASE COUNT         4689 a        11224 c        11536 g         4871 t
ORIGIN      
        1 ctggagatga acaattaggt atgcctaaac taaccgcatg acgacttccg ttcccggcga
       61 gatctccacc gaccgcgtcg cctccgccgc gccccgcgcc gtgtcggcgg ggctcaccgc
      121 gccgcggccc gagggccgcc ccgtgaccgt ggcgggcggg gccggggcgg caccggccgc
      181 cacggagaag acgccgcttc tgggaggcga cgtccccctg gcgcgcatgc ccgcgcactg
      241 gatgctgggc aggctcggca agaaggtcat gcggccgggc ggcctgacgc cgtcgctgca
      301 catgctcggc gccctgcagg ttggggaacg cgacgatgtc gtcgacctgt ggccggggct
      361 cggcgtcacc acgcagcgca ccctcgcggc ccatccgcac tcgtacctcg cgatcgagcg
      421 gggcccggcg gaggcagccc gcgtgcgtcg cctcctcacc cgcgacgagg agcaggtcgt
      481 cgtcggcccc gtgcacaaga cgggccttgc cgacggcgcg gccagcgtgc tctacggcga
      541 ggccctgctg accttggaac ccgccaggcg caagaccgcg atcgtcgccg aagccgcgcg
      601 cctcctgcgg ccgggcggcc ggtacggcat ccacgagctg ctgctcaccc ccgacacgct
      661 gtcagaggct gccaaagacg acatcgagcg caccttgacg aaggttctgc gggtcggcgc
      721 ccgcccgctg acggtcagcg aatggcgccg gctcgtcgaa gaaggcgggt tcgaggtgca
      781 gacggaggag accggcaaga tgctccttct cgacatcccc accttccttt ccgacgaagg
      841 agtctggggc tcgaccctgt tcaccgcgcg ctgcctcgcg cacccgtcgg tcattccacg
      901 catcacgcag atgtggaacg tctttcgccg gtatcgcgac aatctcggcg cgatcgtcat
      961 gacggcccgg cgggcgtgac ccggccgtca tggtcgggtt ctcgctaaag ttgaagggaa
     1021 cccgccgtct gcgaggcctg ggaggagaac gtggtgcctg actcgcatgc ccccggcagg
     1081 gcccaccggc acacggcgca gaaggctgcg atccgcgccg ccttggagca ctccgattcg
     1141 ttcgtcagcg cgcaagacct gcacgccgcg ctgcgcgacg acggcgtgac gctcggtctg
     1201 gcgaccgttt atcgcaacct caacgagatg gccgagagcg gcgaggccga cgttctgcag
     1261 gcggagccga atgtgcagct ctatcgtttc tgcggcgagg agcaccatca ccacctttac
     1321 tgcgtctcgt gcggccgcac gatcgagatc gaggctccga tcgaggaatg ggtcgatgcc
     1381 gtcaccgccg agcacggctt cacgcgcgtg cggcacgtcg tcgacgtctt cggcgtgtgt
     1441 gccgactgcc gcgcctccgg tcgtggtgac gaggcacact gacgcttccg gtcggggtgg
     1501 cgaagcacgc tgacgtctcc cgtcgtggtg acgaagcaca ctgacgcttt caggccggga
     1561 gggcggcggc cgtgtgccgt gtgtgccggg ccgcctgcgt cgaggggccc cgccgtccgg
     1621 cgcccggtct gacgcgcacg gggttccgcg aggcgggccg tgcatgccgg caccggcggc
     1681 ctgtcacaat gcttagttgt aatcattacg attagcgtta tcgccggtag cggcgtagtc
     1741 tctgtctgta ttgagaaagg ttctcgacag caggattccg acgcgcatgt ctgacgcgag
     1801 gggttccggt gcccgtgggt acccggcagg cccctcttgc ggcaggcccg atatggcgcc
     1861 gaggtacggg gcccgaaaca gggtgccggc agcacggcgt ggtggcggca ccggggagtt
     1921 gtgtaacggt gacggcgtcg tggtgatggc gcgtggtcat agaaaaaggg ggcggcagtg
     1981 cgtatcggga agatgatggc cgccggccta gcggcgatcg ccgtcgtggc gggcctcgcc
     2041 ggctgtgctc cggccgaagg gctcgtcggt cgaatgggag ccggcgccgg gggcggggca
     2101 gacaggatcc tcgtcgtcgg ggcagagaac cagtactccg atgtggccgc gcagatcggc
     2161 ggtaagtacg tgaccgcgac ggcgatcatc cagaacccga acaccgaccc ccacacgttc
     2221 gaggcgagcg tgagcgtggc gaaggacctc agcgccgccc ggatcgtcgt gcagaacggg
     2281 ctcggctacg acgacttcat gaccaagctg gagagcgcct cgcccagcaa ggatcgcatc
     2341 gtcatcaccg cccaagacgt gctcggactg ccgaacgaca cggcgaatcc gcacctgtgg
     2401 tacgacccga agacgatgcc ggcggtggcg aagcgcatcg ccgcgagcct cggccgcatc
     2461 gacccctcgc acaaggccta cttcgcgaag aacctggcga cgttcgacgc ctcgatgaag
     2521 acgtgggggg accagctggc cgcctttcgc agcacccacg cgggcacgcc cgtcgccgtc
     2581 accgagccgg tggccgacga cctgctgaag gcggcggggg tagacgtcga gacgccgtgg
     2641 tcgctgcagg ccgccatcat gaacgacacc gacccctcgc cgcaggactc cgccctgcag
     2701 aacgccctgc tcaccggcaa gaaggtcaag gcgctcctct acaacaggca ggtgaccgac
     2761 caggtcaccg agcggttcct gcgggtcgcg cgcgcgaacg gtatccccgt ggtgggcgtc
     2821 tccgagacga tgcccgccgg ctaccactac caggggtgga tggaggcgac gctgaaggcg
     2881 cttcgcgtcg cgatcgagac cggtgcctcc acggacaagc ggtgaagacg acgggccgcg
     2941 cgcggggcgt ctcgtgcaga ggagcggggg atcggtgaag gcacgaagag ccatggtcat
     3001 gcccggcggg gcgggcacag ccgaggcacg ggagccggcg gcgggcgccg tgctcgtggc
     3061 ccgagacatc catgtcgccc tcgccggcag gagcattctc gacggtgtcg acctgacggt
     3121 cggcgcgggg gagttcgtcg ggctgatcgg cgcgaacggc gccggcaaga cgacgctctt
     3181 gcgggttttg ctcggcgtct tgtcgccggg ttccggggag gtcatgcggc cggatggcgc
     3241 cggcctcctg acagatgccc ggcacggccg tggcgtgggg tatctgccgc agaaggtggc
     3301 tctcgacccg gaggcgcccc tgcgcgcccg cgatgtcgtc gccctcgggc tcgacggcgg
     3361 ccgaccgggg ctccggctgc gcggccgcgc cgccatgcgg gaacgcgtcg acgagaccct
     3421 tcgcgccgtg ggcgcggccg agttcggcga ccggcgcgtc ggcgagctct ccggcgggca
     3481 gcagcagcgg gtgcttctcg cgcacgctct tatcagtgac cccgcgctgc tcctgctcga
     3541 cgagccgctc gccaacctcg acccggcgag tgcgcacgac atcgtcgctc ttctcgaccg
     3601 cctgcgccac acccgcccgg tcgcgatcgt catgaccgcg cacgacatga accttcttct
     3661 gccggtcatg gatcgcgtcg tctacctggc cggcggccgt gccgccaccg gggcgccggc
     3721 cgaggtggtg cgcggcgacg tgctcacccg cctctacggg cgacccatca gcgtcatcca
     3781 cgctaagggc cgggtactgg tcatcgccgg cgacgacgcc gcgccggccg acgaggtgca
     3841 ccacgacacg gccttcggtt ccacgggtgg gcagggccgc ccgtgatggc gccggccgcc
     3901 gtgacgggtt ttctcgccag cccgaccgtg cacacggcgc tcatcgtcgg cgggttcgtg
     3961 gccgtcataa ccggcatcgt cggtgtcttc accgtcgtgc gggggcaatc gttcgccggg
     4021 cacgccctcg ccgacctcgg tgcggtcggc ggctccggcg cgttcctcat cggcctcagc
     4081 caagtctggg ggttcgtcgg cgcgggcatc attgccgccc ttctcatgga gtcgatcggc
     4141 gtgcgccgcc tgcaggggcg cgacgtcgcg acaggtgtcg tcttcgcgtt cggcatgggg
     4201 gtgaccgcgc ttttcctgta ttggggcacg acggtgggcg gctccagcaa tgccgccgtg
     4261 tcggtgcttt tcggctcgct cttcgtcatt gatccggcgg tcatccccgt ggtcgtcggc
     4321 ttgagcgtgc tcgccctcgt cgtcgtcgtc gtgtgctacc ggtggctgct cgtcgaatcc
     4381 gtcgaccgcg acctcgcggc ggcccgcggc gtgcccgtgc gcctcaccgg agtcttgttc
     4441 ctcgtagcgc tcgccctcgc ggtcgagctg tcgtcgctga cgatcggggc gatcctctcc
     4501 accggcctcc tcatcgggcc ggcggcgacg gcgctcctcg tcacccggcg cttcggccgc
     4561 gcggtgctgc tctccgccgg cctcgccctg gcgatcacct gggcgggctg cctcgtctcg
     4621 tacgagagct actactggac gggccggcag ggcaactggc cggtcagctt ctgcatcgtc
     4681 gccctcatct ttctcgtcta cctcggggtg cgattaggcg cggcgctggg gcgccggcac
     4741 gcagccgcgc acgccggcgc tcgcccggcc gcgcctcggc ccctcaaggc gggagtctga
     4801 tgtttgcgcc gttcatgacg aacgcctggg tggcaggcac gatcatcgcc gtcgtggcgg
     4861 gtgtcgtcgg cttcttcgtc gtcgcgcgcg gcaactcttt tctggcgcac gccattcctc
     4921 acggggcgtt cgcgggcgcg gccggcgccg tgctcgtcgg cattgaccct ctcctcggcc
     4981 tggggctgtt cgccgccggc ggcgcgctca ccatctcgtt cctcggccgc cgcgcgcgca
     5041 gcgacgtcgt caccgcgttg ccgctcgtca tgatgctcgg gctcggcgtt ttctttctca
     5101 gccggtcgaa ccagtattcc gccgaggtgt tcgcgctgct gttcgggcag gtgctgggcg
     5161 tctccggcgc cgagatcgtg ccgatcctcc tgctcggcct cgtgtgcctc gccactgtcg
     5221 cgctgctgta ccggccgctg ctcttggcct ctgtgctacc cgacatggcg gccccgcgcg
     5281 gcgtcagcac gcaggccgtc tctgtgatgt tcgggctgat cctcgcctgc gcgacgacga
     5341 tgagcgtgcc ggtcgtcggg gcactcttga tgttcgcgct cttggtcggc ccgccggccg
     5401 cggcaggatt cttcgtgtct cgcccgggcc gcgcgatcgc gctcgcggtg gcgttcgcgc
     5461 tcgtcacggt gtgggtctcc gtcgcgttgg cctacgccac cgactggccg gtcggcttct
     5521 tcgtcacggc ggtgagcgcg ctgctctact ttgtcggcag gcttttcgcc gtgctgcgag
     5581 gtcgtcgctc ctttgccatg cgcgacgcgc tcccgtagac taggcaggcc ggttgactgt
     5641 tcgatacttg ttcgcgccca tttttcggct gggagatgcc gactgagaag gggagcgaac
     5701 aagcagcggc ggtcggccgg aatgacgcgg cctgggtcgc tgtgacgtgt gtgagcatgc
     5761 cacagggcgg gtagcggcat agggcggtgg ctcaattggt agagcagcgg tctccaaaac
     5821 cgcaggttgc aggttcgagt cctgcccgcc ctgcacgtcg gcacgagcgg tgccgaacca
     5881 cgaaaggtgt gaccaggtgg cccggagagc agtcgatgaa cccggcgaag acgtcgtcgc
     5941 acggcgcacg cgcggcgatg cggcggcgtc gcaggggttc ttcgcccgca tcatccggtt
     6001 cttccacgag gtgatggccg agctgcgcaa ggttacgacc ccgacgcgta gcgaactcgt
     6061 caacttcacc atcgtcgtgc tggcgttcgt catcgtgatg gtcggcatcg tggcgctttt
     6121 cgactggatc ttcggcatgg tcgtggtgtg ggcgttcggc ggatcgtgat cgggccgggc
     6181 gcgtggcgtg aacacatgag catgagagtg aatgggtagg tagaagatca gtgtctgaga
     6241 cagagcagga cggtgctgag gctacggcga ccgcgccggt cacgacctcc cgagaggcgc
     6301 cggccggcgc cgacccggtg gagcggggcc tcgacgcggc actcgacgcg gtcgcacagg
     6361 cacagggctc cgagccggat gctgacgcgg cagacgatga ggccgacccc tacgaggagt
     6421 tccgcaccga gctgcgcatg aagccgggca agtggtacgt cattcactcg tatgccggtt
     6481 tcgagcgccg ggtgaagtcg aacatcgaag gccgcatggt ctcgatggag atggaggact
     6541 acgtctacca ggtcgaggtg ccgatggaag acgtcgtcga gatcaagaac ggccaacgca
     6601 agatggtcac ccgtgtgcgg gtgcccggct acgttctcgt gcgcatggaa ctgaacgagg
     6661 acagctggtc gatggtgcga cacacgcccg gcgtgacggg cttcgtcggc aacgcccata
     6721 acccgacgcc gctgcgcttc aacgaggcgt tcgagatgct gaagtccctc gtgcaggtgc
     6781 agccgaccgc gaaggcgggg gccgtgggag gccgcggggg cgcggcgcgc accctcgccg
     6841 cggaggtcga cttcgagatc ggggagacca tcaccatcaa ggacgggtcg ttcgccgggc
     6901 tgcccggcac gatcagcgag atcaaacccg agagtggcaa gctcaccgtg ctcgtctcgc
     6961 tgttcgaacg cgagaccccg gtcgaattga gcttcgacca ggtcaccaag ctgtaacacc
     7021 ccgggcgggg gccacgatgc tccccgcctg caccgccgcc cggagatccc gggccgcggg
     7081 agagcatctg cggatgttcg atcgagaaag agagagaaat ggcaccgaag aagaaggtct
     7141 cgggtctgat caagcttcag atccaggccg gcgccgccaa ccccgccccg ccgatcggcc
     7201 cggcgctcgg ccagcacggc gtgaacatca tggagttctg caaggcctac aacgcggcca
     7261 ccgagtcgca gcgcggcaac gtcataccgg tggagatcac cgtctacgaa gaccgctcct
     7321 tcacgttcgt cttgaagacc ccgcccgccg ccgagctgat caagaaggcc gccggcgtgc
     7381 cgaagggctc cggaactccg cactccagca aggtcggcaa gatcacgctc gaccaggtgc
     7441 gcagcatcgc ggagcagaag atgtctgacc tgaacgccaa cgacatcgac gcggcgtcga
     7501 agatcatcgc gggcaccgcc cgctccatgg gcatcacggt cgagtaggcg gaggcaggca
     7561 catcatggca aagaagtcca aggcctaccg ggccgcagcg tcgaagatcg ccgccgacac
     7621 ctactatgcc ccgggcgacg cagtcgccct ggcacgcgag accggctcgg cccgcttcaa
     7681 ctccaccgtt gaagtggcgt tgaagctggg cgtcgacccg cgcaaggcag accagatggt
     7741 gcgcggcacc gtcatcttgc cgcacggcac gggcaagacc gcccgcgtca tcgtcttcgc
     7801 gacgggcgcg gctgccgagg ccgcgatcgc tgccggagcc gacgaggtgg gcgacgacga
     7861 tctgatcgcc aaggtgcagg gcggctggac cgatttcgac tccgctgtcg ccgtgccggc
     7921 cctcatgggc aaggtcggcc ggctgggcaa gatcctcggc ccgcgcggcc tcatgccgaa
     7981 ccccaagacc ggcacggtca cccccgaccc ggcgaaggcg gtcaccgaga tcaagggcgg
     8041 caagatcgag ttccgagtcg acaagcacgc gaacgtccac ttcatcgtgg gcaaggcgag
     8101 cttcagcagc gaccagctct cggagaacat cacggcggcc ctcgacgaga tcgtgcggct
     8161 gcgcccctcc accacgaagg gccgatacat ccagaagggc accgtctcga cgacgttcgg
     8221 gccgggcatc ccgctggacg tgaacgcgat ctgacgccca ctccgacgcg gcgtgacggc
     8281 ggggcccctg agggccccgc cgttctttcg tctccgccgc gcgtcgcgcc cctccaggcg
     8341 acgtgggtgc ccgatgctgc cggcccggtc gcacctgtcg gccacgtcgg cctgctcgac
     8401 gcccccctcg tcatccttcc cagattctca gtcttgccat ggggatgcca tagacggcgc
     8461 tgtgtcgggt gcttgtacgg taaggccgtt caccgacgag gggaaggtta cgggcacacc
     8521 tatggcgcga tggacgcttc gcggaaggct gaggcctcgc atttggatca tcgccggcac
     8581 gatcgtcgtc gtcctggccg gcgccgcggt gtactggttc ggtttcggta tgccgcatgc
     8641 ggcgccggcc gaggcggcga cgcgcacggt gcaggccgcc acgcagacgt tggaggagac
     8701 ggtctcgggc acggggagcc tgcagcccac cgtggacgag gatgtcaact tcgcggcctc
     8761 gggcaccgtg accgcggtca aggttgcggc agggaacacg gtgaagaagg gccaggccct
     8821 ggccacgatc gacaccttga ccgtgcaggc gaatctgttg agcgcgcagg cgagcctcga
     8881 ctcagcccag gcctcgctct ccgacgatga ggcggccggc gacgcgacga ccgcgcaaca
     8941 ggcgaagatc gcggcggaca aggcggctct ccagcaggcc gaggcctccg tgagcgcggc
     9001 gaagaaggcg agagcggcga cgaccctgaa agcgcccgtg gccggcctcg tcaccgcggc
     9061 gaacctgcag gtcggcgacg ccgtgacggc gtcgagctcc tcgaacgcga acgccgcggg
     9121 ggcctcgtcg tcttcgtccg ccgggtcgac gtcgtcgggt cagggctctt cgagcagcgg
     9181 gttcggcggc gtcgggtcgg gcagttccag cagcacgtcg tcgtcctcgg ccggatccgg
     9241 tgcgcagttc gagatcgtgg gaaccacctc gtggcagacc agtgtgtctc tcagcgcggc
     9301 cgatgtgaag caggtcgcga agggcgacca ggtggagctc tccaccgatg acaatccgtc
     9361 gttctttggc acggtctcct cgatcggcct gctgccgacg gtgtcgtcgg gcgccgccac
     9421 ctacccggtc gtcgtgacgg tgaccgggag ccccgatgac ctctacgacg gcgtctcggt
     9481 gaccgcgaag atcgtctaca agcgcattca gaacgctctt gtggtgccga gcctcgcggt
     9541 gacgacccgc ggaggctcca cgagcgtgca gaagatcgtg aacggcaagc aggtcacgac
     9601 cccggtaacc acgggcgaga cctctgggga gttcacgcag atcacgaagg gcctgaaagc
     9661 cggggaccag gtggctctgc agatcttcac tccgacggcc ggtcggggct cctcggatgg
     9721 cggtgcgcca ggtggcgggc agggtggttt cccggccggc agcttcggtg gcgggcagcg
     9781 cggcttctcc gggggcggtg gcttccctgg cggcggtaac ttctccggcg gcggtgcggg
     9841 cggcttcggt ggcggccagg gtggcttcgg cgggggcaac cgtggctgag gcacagacgc
     9901 ctgttgccgg tgcggcgacc tccggtgacc cggtcatcga gctggtcgac gcgggcaaga
     9961 cctatcgcag cggcagcctc gagttcgagg cgctgcgggg gatcgacctg cgcatcgagg
    10021 cgggggacta tgtcgcggtg gtcgggccga gcggctcggg caagtcgacg atcatgaaca
    10081 ttctgggctg cctggacgtc ttgagccgcg gccggtatcg gctgggcggc caggatgtgg
    10141 aggatctgga cgaggtggaa ttggcgagga ttcgtaaccg gcggatcggg ttcgtcttcc
    10201 aacagttcaa cctgctcccc tcgctgtcgg cgctgcacaa cgtggagctg ccgatggtgt
    10261 atgcgggggt cgcgaggccg gagcgggtgc ggcgtgcgcg tgaggcgttg gaaagggtcg
    10321 ggctggggga gcggttgcgc aacaggccgg gcgagctctc cggcggccag cagcagcggg
    10381 tcgcggtcgc ccgggcgatc gtcaccgagc ccacgatcgt cttggccgac gagccgacgg
    10441 ggaacctcga ctcggtggcc acccgggaca tcctcaccct cttcgacgag ctgcacgacg
    10501 cgggtcgcac gatcgtgctg atcacgcacg aggcggatgt cgccgctcac gcggagcgca
    10561 ccatacaggt gcgggacggc cgggtcctcg aggccgacgc cgcggacccg atggtgggac
    10621 gggcgggtgc gcgatgacgt ggctagagac cctgcacacc gcgtggaccg cggtgcacac
    10681 gcataccatg cgctcgatcc tgacggccct gggcgtcctg atcgggatcg cggcggtcat
    10741 tctgaccgtc gggctggggc tgggcacgca gcagaacgtg accgcgcaga tcgacgcgct
    10801 cggcagcaac ctgctgatcg tctccccggg cagcagcacc tcgacgagcg gggtccgcgg
    10861 cgggttcggc accggttcga cgctgacgct ggcggatgcc gaggcgttgc agtcgaaggt
    10921 caacgctccg gacgtgaagg cggtcgcccc gcagaagagc acggagctct cgctgacggc
    10981 cgggcaggag aactggacga cgagcgtcgt cggggtgacc ccgtcctggt tggcggtgcg
    11041 cggccggagc ttgtccgagg gatccttcat cacggcgaag cagaatgccg accaggccga
    11101 cgtggtcgtg ctgggcaccc agaccgccga agagctcttc ggcacgacgc gggtggtcgg
    11161 ccagcaggtc acgatctcgg ggcagagcct cgaggttgtc ggcgtgctgg cctccgccgg
    11221 ctcgaactcc tccagcaacc tggacgacat ggccgtcgtg ccgatatcga ctgcggcctc
    11281 gcagatcatc ggcggcacga accaggacag cgtctccagc gtctatgtcg aggcgacgtc
    11341 gcagtccacg ctctccgccg ccgaccagga gacgacgaat ctgctgctga acctgcacgg
    11401 catcgcgacc tcgaccgacg cggacttcac ggtcagcagc caggactcgc tggtgagcac
    11461 ggcgacggcc gtctaccgca ccctgaccgt gcttttgacc ggcatcgctg ccctctcgct
    11521 catcgtcggc gggatcgggg tgatgaacat catgctcgtc tcggtcacgg aacgcacgaa
    11581 ggagatcggc ctgcgtaagg cgctgggcgc gccgccgagt gcaatccgct ggcagttcct
    11641 ggtcgaggcc gcgatcctcg ggctgtcggg cgggatcctc ggggcggcgc tcggcatcgg
    11701 ctctgccctg gctctgccgg gcgtcatcgg cagcccgatc gtcgtctcgg cgccggccgt
    11761 cgccggatcg atcgcggtcg ccgtcgcggt cgggcttgtc ttcggcgtct acccggtggc
    11821 acgcgcggcc cgcctggccc ccatcgacgc gctgcgctcc gagtgagagc ggcgccgatg
    11881 acctttccca ccgcatccat cacccccgcc gtgcctgtca aggagaaccc catgtcctcg
    11941 tctcgttccg cccgctgcat cttcgccgtg gcggtcgcct tggcggtgcc gctcgcactc
    12001 gtcggctgca gcactggctc gcatgccaag gcctccccga ccaccgcggc ggcgcaggcg
    12061 ccgtcggcat cggggggcgg cgccggcggg cgaggcgggc agggcttccc cggcgtctcg
    12121 ggtgagatcg ccgcgatcca gggcagcacc ctccaggtgc agggctccgg ccagcagacc
    12181 gccgtcacct acaccgactc gacggcgatc acgcagacgg tgaccggctc gctctccgac
    12241 gtgtcggtcg gcgaatgcgt gacgggcatc accgaacctg ccggccaggg ctcctccgcg
    12301 tcggcgaagc cgggcaccgc cgcgacgacg gtgaccatct cgaaggcctc cagtgacggg
    12361 acctgcacgg gcgggttcgg tggtggcggg ttcggcggct cgggcggccg gggcggcttc
    12421 agcggcgccc ggcccagcgg attcccaacc ccgcgggccg gttccagccg gccgacgttc
    12481 cggcccggcg cgggcggccg gagcggcttc acaccgccgg tctcgggcgt cgtcaccgcc
    12541 gtgggatcct ccaccatcac ggtgaaagag acccttccgg cccgcggatc ctcgtcgtcc
    12601 tccgcctcgg gaagctcctc gaccaagacg atcaccgtcg gcgcgaagac caaataccag
    12661 aagaacgcga cggccaccgg cagcgcggtg aaggtcggcc tctgcgtcgt cgccggcggc
    12721 aaggccgggt cgaacggcac cgtcgcggcc acgtcgttgc gcctctcccc gaagacctcc
    12781 cagggctgca ccggcttcgg tggagcgggc ttcgggcggg gcggcttcgg gggcgccggg
    12841 ggcgccggcg ccggccaggg cggcgggagc ggcagcaatg actagcgcgc ataagcatcg
    12901 gcgacaccgc aggcgccgca tcgtcgtggg ctccgccctc ctcgtcgcgg tcctcctcgc
    12961 ggccggcggg gcggcctacg ccgccacccg cccgcaagcg cccagctacc gcaccgcggc
    13021 ggcggcgatg ggggcggtgc accagacggt cgatctcgac ggccaggtcg cctccagtaa
    13081 tcgccgcgac gtcgccttcc agacctcggg caccgtgcag gcggtcgacg tgagcctcgg
    13141 ccaggccgtg acggcggggc agaagctcgc cggcctggac tccagcagtc tcgagtccgc
    13201 gctgaccgac gcccagaacg ccgtcaccca ggccaagcag accctggctg acgacctcgc
    13261 ctcgcagacg cagacgacgg aggcttcctc tgcagccgcg gggggccggg cgggcggcgc
    13321 ctcgagcggg cagggcacct ccggcggcca ggcgggaggg gcctctccct tcgctggggc
    13381 aggaggctcc ggcaccgcgt cgtcgggggc gagcatgggg agccatccgt ccaccggcgg
    13441 tcagggcacc tccgcagact ccgccgccgt cgcggcggcc cggaaagcgg tcaccacggc
    13501 gcaacaggcc ctcctggcgc aggtcaccgc cgcgcagaag tctctcaccg cgaccgacac
    13561 catcgtcacc accgcgacca cgacctgtgc gccgttcctc aaggccgcgc tgggaggcga
    13621 ctcctccggc ggtgccggcg gcacgtccag cacggcccag cagctgaccg actgccaggg
    13681 cgtgattgac caggcgttgg cctcgcagaa ggcgaccgcc gaggcacaga gcaaggtgca
    13741 gagcctcgct gcgacgctga acaccgcggt cgcgaagctg caagccctcc tgaccggggc
    13801 gtccaccggc gcctcgtccg gcaagacctc gtccggcggc gcctccccga cacctggctc
    13861 cacgggatcg ccgagcgctg gccgtgacgc gccttcggtc agcctgggca ggggcgcagg
    13921 cgccaccgtc gagctcgccg ccgacgtgag cacctccgcg acctcgcccc aggacgggac
    13981 ggcgcaggac ggatcagcgc agagcgggtc tggccggagt gcgtcgtcgc tgagcggctc
    14041 ggcgagccag ccggcctcgg ccgcgacgat cgtcgccgac caggccgccg tcgcggagaa
    14101 gcaggcggcc gtcgatgtcg ccaagcaggc gctcaccttc gccaccctga cgagcccgat
    14161 cgccgggacg gtcgccgcga tcggcgtcgc cccgggagac tcggtcaccg gcggatcgac
    14221 gacggaggtg atcaccgtga tcggcgcggg cggcaatcag gtcacgtcga ccgcgacctt
    14281 gacccaggtg aaggagctca cggtcggcca gaccgtctcg gtcacggccg acggccggcc
    14341 actctccggc cacgtgacct cgatcggcgt ccagaacgtg tccacagaca cggcggcgac
    14401 cgcctatgac gtcaccatcg atctcgacgg atcctccgca gaccttctga acggcacccc
    14461 cgcccaggcg atcgtgaagg tcgcttccct ccagcacgtc ttgaccgtcc cgacctccgc
    14521 gatccaccgg aacggcaccg cctatacggt ggacgtcctg cagggcggca aggccgtcac
    14581 caaaaccgtc actgtcaagg tgcagggcag catccgcacc gcgatcaccg gcgtctccac
    14641 cggcgcgaag gtgatcctcg ccgacctcga ccagcagatc gccggcacgg attccagctc
    14701 gagctccagc tcgacctcgg ggctgggcgg gctcagcggc ctgggtggct cgactggcgg
    14761 tgcgagccgc ttcggtggcg tcggcggctt ccccgccggt gggttccgcg gccgcaccgt
    14821 cggtcgatga gtgcgtgcgg cgcgccgaca tggtcggcgc gccgcgaatc gtggcatcgt
    14881 ccccgtgaag atcgcgggcg gccgttcggg tggccggccg cgtcattcct cgcgggcgat
    14941 acgcaccgcg accttgccgg cgacgtggcc ggtctcgacg agttcgtggg cgcggcccat
    15001 gtctgccagg ttgaagacct cttcgagatg cacgcgtacg gtgccctcgt cgatgagggc
    15061 gccgatcttg gcgagcaccc gcgcgtcggg ggcgaccttg acgtcggtac ctctgacgcc
    15121 ggcgcgcgcg acatcgtcga agaacgatgg ccagctgccg gtcggcacgt tcacgatgag
    15181 cccgccgggc ttcaacacgc ccagcgaacg cgtgccggtg tcgtcgtgca cgttgccgat
    15241 gaggtcgatc acggcatcca ccgggtcgat gacctcctcg aagcgctgcc gacggtagtc
    15301 gacgacctcg tcggcgccga gggaacgcac gaagtcggcg tgcgatgcgg acgaggtcgc
    15361 aatcacgtgc gcgccgagat gatgcgcgaa ctgcaccgcg aaatgcccca cgccgccggc
    15421 cgcggcgtgg atgagaacgc gccggccggg cgcggccgag gcgagctcgg tgacgcaacg
    15481 ccacgccgtc aatgccgcga tcggcaccgc cgccgcctcc acgtggccga gagtggccgg
    15541 cttgcgggcg acgctgagag acggcacggc cgcgatctcg ccgaagctgc cgctgaagcg
    15601 cggcgacatg atcatgccga atacctcgtc gcccggccgg atggggtgtg ccgcgtccgg
    15661 cgactccaga accgtgccgc tgaagtcgtg gccgagaacc gcgggaaaag ccgcgatcgc
    15721 ggccgacgtg ccgccgcccg cccgggtctt cgtgtcgatc gggttgatgc ctgcggcgtg
    15781 cacgcgcacg agaaactcgg cgctgatgcg atggggggcg gtgacctggg cgatgtgaaa
    15841 aacatcggga tccccggtcc ggtcgatgac ggcggcgcgc atgagcatgc ctccattctc
    15901 tgcggcccgc gaggcacagt ccacacactg tgggctatcg gtgtgcgcct gcccggaata
    15961 tttcctggcc cgcccggccg gccgggcggg caggcgcggg atgctccagg gttcaggcgg
    16021 cgggcgtccg ctcgtggatg atggccagca tgcgctccgc gcggcgcacg agaatctcga
    16081 tctcgttctc gtcgcggtcc acccagcggc aacgcggctc gggctccacc ggcacgaagt
    16141 cgcgatgctc ctcccacacc acgagggtgc gctcggcgcc caacacgtat tgctgccacc
    16201 acacttgacg caggtacggg cgggggatgt ggcgccacgg cttggtggtc gtcttgatct
    16261 cctcaagctc cagccgtccg tcgggccgga ggatcatgcc gtcaggggtc gccaggtgct
    16321 cggcgctcgt ctcggagcgg aacagcagcg tgctcgggtt cagcccgtaa cgccgtctcg
    16381 cccactcggc gacgatcggc tcgcgcgcct tgccgtgttc cgtgtaggcg ttcccggtga
    16441 aggtcggcgg atgcagcttg tgccaggcgg cctgcaccac ggagcggtcg gtggcgaggt
    16501 gcgccgcgtc cgtcgccgtc acgccgcgca ggcgggcctg aagccaggcg gcccggtgcc
    16561 cggagtcggc gacgacccgt cgacgcagac tctccgcaag cacggcctta ttctctcgcc
    16621 ggcgggcctg atcgcgggga ccggccgtcg ctcggcgtgc ctcgcgcctc tggccggccg
    16681 cgatcatcga agattcacag tctaaacaaa ggcgccgtgt agcgggttct ctattcgcac
    16741 cttgatactg gcaggcgtga gcatcaaaag cgttgaggac aaggcgggtt ccggccaccg
    16801 cacccaggct gttctgcgtc gcgcgggcgg cggcaaggtt cgtgttctcg tcgtcgacga
    16861 cgagaacacg ctgaccgacc tgctttcgat ggcgttgcgt tacgaggatt gggaggtgca
    16921 tacggccgac accggccaga aggcgctcgc ccaggcgcgc gagtttcgcc ccgacctcgt
    16981 cgtgctcgac attatgcttc ccgacatcga cggtttgacc gtgctctccc ggttgcgcag
    17041 tgacggcttc gacgcgcccg tgctcttctt gaccgccaag gatgcgctcg acgatcgggt
    17101 ggcgggcctt actgccggcg gcgacgacta tgtgaccaag cctttcagcc tggaagagct
    17161 cgtcgcccgg ctgcgcgggc tcctgcgccg ctcccgcatc gccgtgcccg agacctccga
    17221 cccggtgctg gtcgtcggcg acctcacgct cgacgaggac agctacgagg tgacgcgggc
    17281 gggggagagc atctcgttga cggcgacgga gttcgagctg ctgcggttcc tcatgcgcaa
    17341 cccgcgccgg gtgctcagca aggcgcagat cctggatcgg gtgtggagct acgacttcgg
    17401 cggctcttcg agcgtcgtcg agctgtacat ctcgtatctg cgcaagaaga tcgacgccgg
    17461 ccgcgcgccc atgattcaca cggtgcgtgg cgccgggtac atggtcaagg ccgcgagcgc
    17521 atgaccggcg gcgcgcgtgc cgcgccagag acaggggcct ccccgcgccg cccggcgctg
    17581 ctgcggcggc tcgcggccgc tgcgaggctg ggcgcctggt cgctgcgtgc ccggctcgtc
    17641 gtgacggccg tcgtgatgat ggcgctcatc agcgtgatca tcggcgtgct cagcgtcttg
    17701 gccctccgca actcgatgat ggccgacctc gactcccagc tacacagcac gatcgaccgg
    17761 ggtcgggccg tcgtgttgca cgagatgcag aacttcgcgg cgcccggcag gccgctgatc
    17821 ggggcggtcg tcgcggcgac cggccagccg acgggaacgt tcggtgccta cgtcaccgac
    17881 cagtcgatca cggtcgccta cctcgacccg gccgcgacga cgaagaacgt cacccgcaac
    17941 ttgtcgacga cgctcaccga cattcccgcc gatcagggtg cgcagaccgt cgacctgggc
    18001 ggcgggttcg gcgactaccg gctcgcggcg acccgggtcg ggcccggggt gcttgtcata
    18061 gggctgccgg tggccggggt cgaccagacc atctcgcgcc tcatcgtcac gatcgtcgtc
    18121 gtctcgctgc taggcctcat cgtgctcgcc gtggccgcgg cgatgatcgt gcgcctggcg
    18181 ctcagcccac tggagcgcat ggccgcggcg gcccgcaagg tctccgagtt gccgctcgac
    18241 cgcggcgagg tggcgttggg ggtgcgcatg ccgcagaagg atgcgcagat gcacaccgag
    18301 gtcggcatgg tcgcggccgc gttgaaccgc atgctcggcc acatcgccgc ggcgctcgcc
    18361 gcccgacaac gaagcgagca gaaggtgcgg gagttcgtcg ccgacgccag ccacgagttg
    18421 cgcacgcccc tcgcctcgat ccgcggctac gcggagctga cgaggcgcag cccgcaggag
    18481 ctgccggccg acgcgggccg ggcgctcggc cgcatcgagt cggaggccgc gcggatgacc
    18541 gggctcgtcg aagacctgct gctcttggcc cggctcgacg cggggcggga ccttgagtac
    18601 ggggtcgtcg acctgacgag cctcgtgatc gattcggtca gcgacgcgca cgccgccggc
    18661 cccgggcatc ggttcgagct ggacctgccc gaggagccgg tggagatcta cggcgatgcc
    18721 gcccggctgc atcaggtcgt cgtgaacctc ctcgcgaacg cgcgggtcca cacccccgac
    18781 ggcacgaccg tgacggcggt ggtgcggcgg gaggacggcc gggcggcagg ggctcccttg
    18841 gctgtgccgc acgccgtcgt gtctgtggtc gacgacgggc cgggcatccc cgagcctcag
    18901 caggccaggc tcttcgaacg gttcgcgcgc ggcgacgcct cccgctcccg agccaccggc
    18961 agcacgggcc tcggcctcgc gatcgtgaaa gccgtggtcg acgcccatca tggtcttgtc
    19021 aaggtgtcga gcgtgccggg ccgcacggag ttcacggtgc ggctgccgct cgactcgccg
    19081 cccccgccgc cgaacgcctg accgttcggc gggccgcgcg aattgcgtgg cggccggcgg
    19141 ccccgtaaag tagtgggtgc cgaagaccgc tggttgtcgg gtgcacgcat gcgtggcacc
    19201 tgaccgaagg ttcgtacgac gaacggcccg cgcaggtgag tagggattca cgtcgaaaga
    19261 cgtacgagct ccgtgccgcc tgggccgggg cttttttcgt gttcgcacca agggccgcca
    19321 gcacctaatc acaaggagtg gccatggcgg acaagcacgc cgcggttgcc gagttgacgc
    19381 agaagtttcg cgactcgacc gccgtcctct tgaccgagta tcgcggcctc actgtcacgg
    19441 cgatgaagaa tcttcgtcgc agcatcagtg cagacgcgac ctacgccgtg gtgaagaaca
    19501 cgctcaccaa gatcgcggca cacgacgccg gaatcacggt gttcgacgac cagctcgccg
    19561 ggcccagtgc gatcgccttc gtacacggcg accccgtcgc cgctgcgaag ggtctgcgtg
    19621 acttcgctaa cgacaaccct ctgctggtga tcaagggtgg ctatttcgat ggtcacgccc
    19681 tgaacgccga cgaggtcaat cagttggccg aactcgaatc ccgcgatgtg ctgctggcca
    19741 agctggccgg cgctttcaag gcttcgctgt tcggcgccgc atacctgttc aacgcaccgc
    19801 tctcgaaggc cgttcgcacg gtcgacgcgc tgcgtgagaa gcaggaagaa gccgcgtagc
    19861 ccacaccggc gcgcatctga cccacgaaga ctgcacacga caaggagacc atcatggcca
    19921 agctttccac cgacgagctg ctcgacgcat tcaaagagct gaccctcatc gagctgaacg
    19981 aatttgtgaa gaagtttgag gagaccttcg aggtgaccgc ggccgcgccg gtcgccgtcg
    20041 cggcgcccgc ggccgcgggt ggcgcgggcg agcctgccgc agaagagaaa gactcgttcg
    20101 acgtgatctt ggagaccgtc ggcgacaaga agatccaggt catcaaagag gtccgctcgc
    20161 tgaccagcct gggcctcggc gacgcgaagg cgctcgtcga cggtgcgccg aagcccgtgc
    20221 tcgagggcgc gaacaaggaa gacgcagaga aggcgaaggc caagctcgag gcggccggcg
    20281 ccacggtcac cctcaagtag ccgtcgcccc cggaagaccc agggggctta ggcgacatcg
    20341 accgggcggc attctttcgg gggtgtcgcc cggccggctt ttaacataga tagatgtgct
    20401 atgtataatg ggctccgtga taccccctga acccgacgca aggacggcgc gcctgcaaga
    20461 gaccatcagt gatctcatcc aggtcagcca tcgcatggcg cggttggccg cccacgtctc
    20521 cggcagcacc gagtcaccgg cttcgtggcg caccctcagc gtgctccagg cctacggacc
    20581 gatgcggctc ggggagctcg ccgagcgcag ccgggtcagc cagccgacga tgaccaagct
    20641 cgtgcggggc ctggtcgatc ggggccgcgc ccgccggctc gccgacagcg acgacgctcg
    20701 agcctggcag atcggcatca cggccgacgg tgatgaggcg ttggagggtt ggcggcatac
    20761 catcgggacc gcgctcgcgc cgcttttcgc cgacatcacc gacgaagaac tgcgtaccct
    20821 gcaagaaggc gtgcgtatcg tcagcgccgg cgagcgtcgt gccgacggca tcgacctgct
    20881 cgggtcggcg aagggttcgg ccaccccgcc ccggggggcc tcgccggcgg cgcccgccgc
    20941 ggggggtgcc ctgtgagcgc cgacacggag acgtcgcccc tcgtccacgc cggcgacgac
    21001 accgagttct cgggggcacg gccggccccg agcatcctcc accagccgac cgccgtgtgg
    21061 gcggtcgcct tcgcctgcgt catcgcgttc atgggcatcg ggctcgtcga cccgattctg
    21121 ccggccatcg ccgcagagtt gaaggccagc ccgacgcaga ccgagctgtt gttcacgagc
    21181 tatctgatca tcaccgccat cgcgatgttc ttcagcagct ggatctcgag tcgcatcgga
    21241 gccaaacgca cgctcctcat cggcctcgcc ttgatcgtgc tcttcgcggc cgcggccggc
    21301 gccgccggaa acgtcgaagg cgtcatcggc ttccgtggcg ggtgggggct cggcaacgcg
    21361 ctgttcatct ccaccgcgct cgccgcgatc gtcggcgcgg cctcaggcgg tgcgtcctcg
    21421 gcgatcgtgt tgtacgaggc ggccctcggg ctcggcatcg ccgtcgggcc gcttttgggc
    21481 ggccttctcg gcggcatcag ctggcgcggc ccgttcttcg gcacggccgt gctcatggcc
    21541 gtcgcgttcc tgtcgatctt gatcctcttg aagcccggcc gcgcgcgacc cgcgccgagc
    21601 ccgctcgcgg cacccttccg ggcgcttggc aggccgggtc tcaccgtcat ggccgtcgcc
    21661 gcgctcttct acaacttcgc cttcttcgtg ctgctcgcct acacgccctt tgcgctcgtg
    21721 ccgctcggcc ttgccagcgc gatgaccctc gggctgatct tctgcgcctg gggcgtgacg
    21781 ctcgcgatca gctcggtgtg ggtggcgccg cttttcacga ggcggatgcg acgcacgcgc
    21841 gtcatgtggc tggtgcttcc gttctttgcc gccgacatgg tggccgccgc cttcgtcatc
    21901 cactccggcg cggccctgat cggctgcgtc atcgcgggcg ggctgttcct cggcatcctc
    21961 aacacggtga tgacgcagtg cagcatggaa tcgaccgatc tgccccgcat ggtcgcctcc
    22021 tccgcctatt ccggggttcg attcttgggc ggcgcgatcg cgccgccgct tgcgacgatt
    22081 ctggcgaggc tcgtcgaccc cgcgacgccg ttctacgtgg ccgcgggggc gttgctggtc
    22141 agcctcggca tcaccgccgt gggcgtcaag gcgctgcgcc gggcagacgc cgcgccggcg
    22201 tcgtaggcac atcgccttgc cggccgtgcc gcgagccggg actcatcgcc cgctttcggc
    22261 gcccctaggc tgagaggcat ggcggcgttc gcatcggggt ggatgcggga actggtgcga
    22321 ccgaagagcg cgccggtgcc gtgggcgcgg gtcttcaggt gtgctgtctc catcacggcg
    22381 cccgtcgccc tcggcgccgt cgtcggccgg ctcgacatcg gcctgctcgt cgcgatcggc
    22441 ggcctcacgg gggcgctcaa cgaccgtgat gcgccgtacc tggccaggat gacccgcatg
    22501 gcagtcgtcg ccgtgggcgg caccgtcggt ttcgggctcg gcggcctggt cttgggcgac
    22561 gaggtgctga cggccgtctt cgtgctggcg ggcggcctcg tcgccgcgct cgtgagtgtg
    22621 ctgggcggcg ccgcctcgtc ggccgccctg cagttcctca tctacctcat cgtcgcttcg
    22681 ggcgtcgact tcgggcccgg cccggcctgg ctggggccgg cctgttacct cggcggcatc
    22741 gcctgggcgc tggcgctgtc tctcgtgggt ggccttggcc gctcggccgc gcccgaacgt
    22801 ctcgccgtgg ccgaggtgta tcgatgcctg gcgctcgtca tggagtcggc cggcgggccg
    22861 tcggcccagg cggacgccgc ccggcagaag ctcaccggcg cgatgaacga ggcctacgac
    22921 gccgtcgcca cccggcgcac gctgagcgcc ggccgcgact cccacgcccg actgctcgcg
    22981 gcccttgtga atgccgtgac gccggccgtc gagacgacga cggcgctcgt gcgttccggc
    23041 acacgtgtgc cggtgtcttt catcgcccgg gtgcgagaga tgtcgtcgga gatccgccgc
    23101 ggcgcgcgcg gcgtcccgcg ccccgccggc tgggtcgtct ccgcacagca cccgcgcgcc
    23161 gcatggctcc ggcttctgca cgacgagctc gacacggtcg acgagatcct cgaggccacc
    23221 cggcacgccc ggcggccggc ccggccggcg acgaccggcg gcgtcacccc ggtgacgcgc
    23281 gccctgaaga tggccggcat gaccacctcg gtacccgtct cgggcgccgg gcggcaggcg
    23341 atccgctcac tgcgtgacgc ggtggcctcg gggccggcga cctggagccc catcgtgcgc
    23401 ctggtgctct gcctcgcggt cgccgaggcg gtcgccatcc tcgacccgga gcctcgcccg
    23461 tactggattg ccatgaccgt cgcgatcgtg ttgaagcccg acttcggcag cgtgttcgcc
    23521 cgcgccgtgc agcgcggcac gggaacgatc gtcggcgttc tcctcggcag cgtcgtgctc
    23581 gctctcgcgc cgggtgtcgt gttgccgatc gtcgccatcg cgatcctttc ggcgctcctg
    23641 cccgtcgccg tcggccgcaa ctacggcatg tatgcgacgt ttctgacgcc gctcatcgtg
    23701 ctgctcctcg acctgggcca gggcgcctcc gggcccggcg gcgcacagtt ggtcgtcagc
    23761 cgcctcgtcg acaccgtcct cggctgtgtc atcgtgctgc tcgtcggcta cgtgccgtgg
    23821 cccgatacct ggcgttctcg tggccggctg ggtgcccgct tcgccacggc ggtcgtcgcc
    23881 gtgcgcgact acgtgaccgc ggcgctcggc gcccccgccg accccggccc cagccggcag
    23941 tcggcgagaa ggacggccta tcgggcgctc tccgacctgc gcacgtacct gcagcagagc
    24001 ctcgccgaac cgccggccgt gagtgccgtc gcggccggct ggtggccggc cgtggtcgct
    24061 ttggaacggg tcaccgacgc cgtcacgggc gttgtcgtcg gtgctcgggc ggggcgggcc
    24121 cggccgggcg ccgacggcgt gcggagggtc gtgaccgcac tcgacgtgtt ggccggccgg
    24181 ctgcggggcg agcccgccga ggcggccggt gccgcgacgt cgtcgaccgt caccgacccg
    24241 gccctggccg gcgtctgggc agagttgcgc accgcccagg cggttttcgg cacgcgcacg
    24301 ccgcccccgg gcccttagta ggctggcgac atgacgagca ccggcgggtt cgcaacccga
    24361 gcgatccacg cgggccaaga gccagatcgg cagacgggcg ccgtggtgcc gccgatctac
    24421 cagacgtcga ccttcgtgca agatggcatc aacaagctgc gcggcggtta cgaatacagc
    24481 cgcagcgcca acccgacccg cacgatgctc gagagcctgc tcgcctcact ggagggtgga
    24541 gcgcgcgccc tctccttcgc ctccggcctc gccgccgaag acgcgctgct gcgcaccgtg
    24601 ctcgccccgg aatcccgcgt cgtcgtcagc cgcgacgcgt acggcggaac gtaccgcctg
    24661 atcgaccgcg tgcacggcgc gtggggcgtg cgcaacaccc ccgccgacct gaccgacctc
    24721 gcggccgccc gcgaagcgct cgccgccgca gcggtcgacc tcgtctgggt cgagacgccc
    24781 agcaatccgc tcctgaccgt caccgacatc gcggcgctcg ctcgcctggg gcacgaggcg
    24841 ggcgcactcg tcgtcgtcga caacacgttc gcctcgccgg ccctgcagaa cccgctcgcg
    24901 ctcggtgccg atgtcgtcgt gcactccacc accaagtacc tgggcggcca ctccgatgtg
    24961 ttgggcggcg ccctcgtctt cgccgacggc gacctcgccg agcgggtcgc ggccgttcag
    25021 ttctctgccg gtgcggtgtc gtcgccgatg gatgcctggc tgacggtgcg cggcatcaag
    25081 acgctcgccg tgcgcatcgc ccggcactgc gccaacgcgc aggcggtcgc cgagcatctg
    25141 gccggccatc cggccatcga gacggtctat taccccggcc tcgacaccca cccgggccac
    25201 gcgctcgcgg cccggcagat gcgcgggttc ggcggcatgg tctccgtcgc cgtgcggggc
    25261 ggcgccgggg ccgcgatgcg cctcgtcgag tcaacccggc tgttccagct cgccgagtct
    25321 ctgggcgggg tcgaatcgct catctgctat ccgtcggaga tgacgcacgc ggcggtgaag
    25381 ggcacgccgc tcgccgtcgc cgacaacgtc gtgcgcctct cggtgggcct ggaagaggcc
    25441 gacgacctca tcgccgacct cgacgccgcg ctggcctagc gcaccggggc ggccgcggga
    25501 gagtcttgcg tcgtcggcgc ctcggggccg ggcgcggccg ccgggacacg cagccgcatc
    25561 acgaggatcg ccattgtcgc ggtgatgagc gccgccagca gcatatgcga ctcgatggcc
    25621 accgcaggca ggccgtcgcg cgcctggtag atgccgagcg gtatctggat gagcagcagc
    25681 acgagcaggc cgatcgacca gccgagcggg cgcagccttg cgcgtgccat gccgatgacg
    25741 agcacgatcg agacggcgag cagcgcgtag gcgggccccg aatgcacgtg cgccatgacg
    25801 acggcgttga acaggtggtg gcgcacgtcg gcgttgcccg agtgcgggcc cgattccgag
    25861 gtgacgacgc cgagaatcaa cgtcagagcg gtcagcaccg ctgccgtgtg tgtcatgccg
    25921 acgatccagc ggggtgcggc ccagcggcgc gggccgggcg ccagcggggc acgggccacg
    25981 aagaccgccg cgacgatgat gaggatcatc gaaatgagaa agtgggagcc gacgacgtcg
    26041 tagttgaggt gtgagagcac cgtcacgccg cccatgagcg cctgcagcaa cacgccgccg
    26101 atgacgatcc acgcgagggt gaagaggtct cgcctctctt tgcgcatccg ccacacgagg
    26161 agcagcacgg ccaaggcggc gagtcccacc acgccggaca tcatccgatt gccgaactcg
    26221 acgtaggagt ggaagctcat gcccggcacc ggcacgagcg agtcgggcgc gcacaacggc
    26281 catttgcagc cgagccccga accggtcagc cgcacggcgc cgcccgtgcc gatgatgagg
    26341 atctcggtga cgagggagac ccaaccggcc acgcgcacgg atcgcggcgc ccccgccgcc
    26401 cccctgccga gtcgtgcgcg ggtgggggtg gaaaggtcag cggacatgaa gcctccgttc
    26461 ggccgccttc gtgatgccgc ggggcctgac gcgtcgtgct gtcggaagcc tggatgcgac
    26521 gcgggtcgcg gcatgccggg ctcgccagaa agtctacggg gtgggtgtgt ggagaggcgc
    26581 ttcccccgac cgttctgcgg cggagcatcg ctttgtcacg gcgcccggcg ccttccgcat
    26641 gcagaatatt gccaggaagg ccacagggcc ggaggatcga ataggggcgg gcagtccgtc
    26701 gcgcggcatg caggtactcg ggtgccgtgg ggtcgcctcg cgcaggcgcc ggtgcggccg
    26761 cccgcacggc agcgcacgcc gggcggaggg gagaaggaac gatgaacggt gtcgaggctt
    26821 tggccgacgg attcggccgg ataagcggcg tggtgcacgc ggtgctcgac ggcatctcgg
    26881 atgaggcgct gacttttcgc gccgaccccg acgcgaacac ggtggcgtgg ctcgtctggc
    26941 acctggctcg cagccaggac gcgcaggtgg cgcacctcgt cgccggcgac gcgggtgccg
    27001 gggcggccga cgccgccggg gcttggcagg cggagggatt cggcgagcgt ttcgcgctgc
    27061 ccttcggccc gggggagaca ggttacgggc atacgagcgc caaggtcgcg gcggtgcgtg
    27121 ccccggccgg ccttctcggc gaatattacg acgccgtcgc cgcccgcacg acggcctatg
    27181 tcgcggggtt ggccgacgcc gacctggatc gggtcatcga cacctcgtgg accccgcctg
    27241 tcacggtcgg cgtgcggctc gtcagcattt tggccgacgg ccttcagcac gccggccagg
    27301 cggccttcgt gcgcggggtc gcccagcgcg tacgctgacg ggccctggta gaaggccctc
    27361 gccccgtagc attcagtcat gcgcgtcgtc atcgcccccg actccttcaa gggcactctt
    27421 ggcgccgccc aggtcgcagc ctcgcttgcg gccggctggc gcgaggtgcg cgcggcagac
    27481 gaggtcgcct gcctgccgat ggccgacggg ggagaaggga cgctcgacgc gatccgcgcc
    27541 gcccggccgg acgcgcggcg tatgcccgtc gccgtgcagg gtccggacgg ccgcacgcat
    27601 gcggcggaat ggctgctcct tcccgacggc ggcggtgtcg ccgacgacca cgaccggaat
    27661 ggcgacgacc ggggcggcgg catcggcgtg atcgaactgg ccgcgacgag cggcctcacc
    27721 ctgctcgatc gcctccggcc gttcgacgcc cacacggtgg gcttcgggca ggccatcgcg
    27781 gccgccctcc ggcacggcgt gcggggtctg ctgctcgcga tcggcggcag ttcctcgaca
    27841 gacggtggga cgggcatgct ccgtgccttg ggtgcgcggt tccaagacgc cggcggcgct
    27901 ccgataccgc tcggtggcgg agggctcgat cgcctcgtcg tagccgacct cgacgggttg
    27961 ccggccctgc cgccgggcgg cgccgaggtg ctgagcgacg tcacgaaccc gctgtgcggg
    28021 cctcgtggcg ccgccttcgt ctatggcgag cagaaggggg cggatgcggc ggcgcggagc
    28081 cggctcgacg cggggctcgc ccacctggcc ggcgtcgtcg gccaggcgcg ggcgagcgat
    28141 ccgggcgccg gcgcggccgg cggcaccggg ttcggcctgc tcgtctgggg tgaccacgcg
    28201 gcgaagcggc gcgacgcagg ggcagagggc gaggcagccg gccggcttgt catcgtctcc
    28261 ggcgcacgcc gcgtcgcccg cgcgataggg ctgccgcagg ccgtcgccgg cgccgacgtc
    28321 atcgtcacgg gcgagggccg gttcgacggc cagtcgtcgg agggcaaagc gccctggctc
    28381 gtctccggtc tgccgtcgcc cgcccgccgg tatctcgccg cgggcagcat cgaggcgccg
    28441 acgcccggtt tcgctgccgc cgtctccctc gtcgatctcg cgtcggggct cgccgcagcc
    28501 aaggcggagc cggagcgttg gcttcagcgc gcgggcgcgg aactggcgcg gctcgcccag
    28561 cgggagaact gatcacgcac ctccggccgc gggtcggcct cgcgctcggg tgtgagaggc
    28621 gtgtgccacg acgcccggcg gtgggtcttc gcccacaccg cctgggcggc ggccccgtcg
    28681 gcgacgatgc tgcgggccgc ggccggcccg gatgccgccc cgccggcggc ccgtacggcg
    28741 gtgggcgacg aggcggcgga tgacgggccg gcggcttgtg gcacggcgac gggaaggccg
    28801 aagacctgcg ccgtcgcctg ctgcacgacg ccccgccccg ccccgggccc gatcacgatg
    28861 acgcgcctgg ccggcaggcc tgccgtcgcg agggcgtctg ccgccgcggc gagggtgcac
    28921 agcacgcctt cgagggcggc gcgcgcgagg ttctcgggcg tcttcgccgt ggcgtcgggt
    28981 gcgtggaatt ctgccgtgcg cggcgtgaag acgaccccgc ccgcgccggg ccgggcggcg
    29041 ccggcaagcc gggcgaggcc gtcgttcgtc acgccgagaa ggcgggcgac gccggccagg
    29101 gccgaggcgt cgcccggcgt cacgacggtg ggcaggaacc cgccggcggc gtcggccagc
    29161 gagcgtatcg caccggtctc gtccacgacg ggggcgggcg ccatcgtgct gaccgccccg
    29221 gagcccagcg agaccatgat gtcgccgggc gcaagaccca agccgagtgc agcgatcgcc
    29281 gggcgggtgc cgccgatcga gatcacgcgc tccgacttga tgccgtgcga cggcatcttc
    29341 cagctttcgc cgccccaggc gtcgggaggc atgacccgtg gctttatggc gggccggccg
    29401 agggcctgcc cgaacaggtc gtgatcccat ttgccggtcg cgggattcca gtagccggtg
    29461 cccgaggcgc cggaacggtc ggtgacgagt tcttccaggc gcagaccgtt cggcatctca
    29521 tcgcgcggcc cgaacccgcg cagccgccac atcagccagt cgtgccgcag ggccacagcc
    29581 tccacccggc cggcgttctc cggctcgccg tcgcgcagcc agcgcagttt cgccagcggg
    29641 tggctgccgg ccggaatcga tccgacccga gccgcgaggg cctgcctgcc gacctcggcg
    29701 acgaggtcga gggccgcgcg ggcgaagccc cgctcgtcgc ccacgagcgc ggcccgcacc
    29761 aggtgacctt cggcgtcgag ggtcacggcc tgcccgtcgg ccgcgccgat gccgaacgcc
    29821 gagacgtcgg cgaaccctcc ggaagcctcg atggcgtcga gcagagcctg ccaccaggcg
    29881 tgcggatcgg cctcggcgcc ggcgggcgcc gccgcccggc ccgaacggac ccgctcgccg
    29941 gtggcggcgt cacgcacgac gacggtgcaa ccgcccgccg ccgagtcgat tccggccacg
    30001 agcgttctcg tcgtgcggac gcccgagggc cccgccccgt gggcgcgcgg cgtgatgcgg
    30061 tgcacccgca cgggcgcagc cccgtctgtc accgccggca tcgccccacc ttccaccccg
    30121 cccgtaacac tctgtcacac ccgcgggcga gggcggtcgt cgggtctcag gcccgatgcg
    30181 ccgtgctccc agaagactgc cccgcgtcgt cgctggccgc gtcgtcgctg gccgactccc
    30241 gtgccgcgtc ggccacgtac tggtcgataa gcctcttcgg cagcgtgatc ttgagagcca
    30301 gccagtagat gaacgccgag acgaccgcgt tcacgaggaa cacccagaag aacgagtgct
    30361 gcacccacgc ggtgccgaag acggcggagt cactgaagaa gaagctcacc aggccgtaca
    30421 gcgcgaacca ggggatgatc catgccgcgc ccaccttgaa gagcagcggc ggccggccct
    30481 ccttgtgccc gaagagctgg aagagcacga gcaccgcgta gccgatcaag atggtgatga
    30541 tcaccttgaa gttggtgctc cagcccgccc agaacatgat catgttcgcg ctgaagaacg
    30601 cgaggtaggg gatgatgtcg ccgcccggca gcttgaaggg ccgcttcagc tccggatgcg
    30661 tgcgccgcag cacggcgacg accagcgggc cggagccgaa cgacatgacg gtcgcgctcg
    30721 tgatgaagcc gacgagggac tgccagctgg ggaacggtgc caggagcacc acgcccacca
    30781 ggaacgtcac gagaaggctc cagtgcggca ccgcgtactt gttgttcttc gcgagaaacc
    30841 gcggtgagtt gccgttgcgg cccatcgcat acgagaggcg ggaggtgacc gtcgtgtaga
    30901 tgaagccggt gtcggcgggg gagatgaagg cgtcgaagta cagcacgtag gccagccacg
    30961 cgagccctgc catgccggcc aacgccgcca gcgggccgaa gtcgccgctg aactgcaggc
    31021 cgtcccagcc gtgcgacttc acgaggacgc tgtgcggcac ggcgagcgtg tagacgacct
    31081 ggatgagcgc atacaagacg gccgtgatcg cgacggagcc gatcaaggcc aaggggatgt
    31141 tccggcgcgg gttgctcgtc tcgcccgcca actcgatgcc ttgccggaag ccgaggtacg
    31201 agaacgttat gcccgcggtg gagatgcaga tgaacacgcc ggcgaagccg gtgggcgcga
    31261 agccgaattg gctgaagttg gccggcgacc ccatccgcgt cgtgccgaac gcggtgatga
    31321 ggaagacgac gatcaccagg cagacgacga acagcttcca ccacacgacg accgtgttga
    31381 tctcggcgaa gagcttgacg ccgaagtagt tgacgacgac gaagagggcg aggagcagca
    31441 cggcgacgac gattccgagg ggcgtcaaga tctgcgcggt cgtgctgccg gaggtcgcct
    31501 tcgtcgtgaa cggcgcgaac ttcgtcatgt aggtcaaggc gccggtgacc tcgatggcgg
    31561 gcacggctgc tgccgcgatc caggtgatga agccgaggga gtagctgccg aaggagcccc
    31621 acacgaggtg cgggtaacgc acgacgccac cggagatcgg cagcatgggc ccgagctccg
    31681 cgtagcacag gccgatcagg atgatcatga cggccgcgat cagccaggag aagatcgcgg
    31741 cgggcccggc gatcgccgag gcctggtacg cgccgaagag ccacccggag ccgatgatcg
    31801 agccgaccgc ggcgaagagc aagcccaccg gcccgacgga acgtttgagc ttcaccccgc
    31861 cagcgtcggt gtgatgcgcg actgtgcttc gcggatggtg cgctgtcgac atgacgtggc
    31921 ctctttagtg ttcgtctaat gggcgggatt cctcacaaaa cgctcctgtg cgtttactgg
    31981 ggggtggttc cctgaggtct tatgcccgga gtctgagagc catctttctg agttttatca
    32041 tttcgccgat accgcgtgcc cggaggaagc ggcccgtttg ggccgcgcaa cgcgtggcgc
    32101 gggtgtccga aaggcgcgtg gcggggcgcc gcgcaggcgc gtccgacccg tcgctgaatt
    32161 tttgctatga atattgaccg gccggtcagt cgatgcctag cgtgagagat gaggaagccg
    32221 atgacggatg ggttgccatc cgccagacac ggagccatct gacggtagga ggatgagttc
    32281 aatgacaagg tggacacgct ggcaggactg ggtcgcagtg
//
LOCUS       DASTNL010000048        10586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_965041, whole genome shotgun sequence.
ACCESSION   DASTNL010000048 DASTNL010000000
VERSION     DASTNL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965041"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFQ96_01615"
     CDS             <1..483
                     /locus_tag="VFQ96_01615"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0256518.1"
                     /translation="GGAKDALPAPGKAETGTELSAAQIETIRKANTFFIGTSHPDGPA
                     DASHRGGEPGFIEVDSPHLLRWPDYNGNAMFMTLGNISLNPSVGLLFPDWDTGGLLQV
                     SGRAQIDWDPASAARLVGAERVVDFQVDAVQETRHALPTRWSPARLSRYNPKPAPVAA
                     "
     gene            complement(443..1066)
                     /locus_tag="VFQ96_01620"
     CDS             complement(443..1066)
                     /locus_tag="VFQ96_01620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0256519.1"
                     /translation="MEWTSFFAASAGRAPRPLVRQVLERRGDLPAGVALDLGCGDGTE
                     ARFLAARGWRVHAYDGAPGTPARVRAGVSGPAAALLTATRAAFEDLASLPDADLVYAG
                     HALPFCPEESFATLWQAVRDCLRPGAWLIADFFGPRDLWVGRTDMSFRGRTQIEALLG
                     GLDAVRIDEVEGPAGTPFGPKHLHLLTAVAHRPVLRRPPAPASGYSG"
     gene            1240..2178
                     /locus_tag="VFQ96_01625"
     CDS             1240..2178
                     /locus_tag="VFQ96_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256520.1"
                     /translation="MTAAIVTRDLHKHYGRLRALDGLDLVVDAGQVHGFLGPNGAGKT
                     TAIRTLLGLARATSGEARVLGMDPWKDAVAIHRRLAYVPGDVSVWPNLSGGEAVDLVV
                     RLRGTAVKDRQYRLERERLMDAFRFDPRKKGRSYSKGNRQKVALIAAFAVPADLYILD
                     EPTSGLDPLMSVVFHHEVTRVAAAGATVLLSSHILSEVEQLCDRLSIIRRGRVVEAGT
                     LADLRHLTRTEVSFAARGITPDRPPGVPGAHDVVVAGGRVRFTLDDDRVAEALPALAE
                     LGVVGLRVTPPSLEELFLRHYGDDPAAAQTAETRRR"
     gene            2175..3797
                     /locus_tag="VFQ96_01630"
     CDS             2175..3797
                     /locus_tag="VFQ96_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide antibiotic transporter"
                     /protein_id="HEU0256521.1"
                     /translation="MRRFAALWRLRVRRDRLQLPLWIVGTVLLAYAGYAGVTESYGSL
                     ADRQGILAAARADPVILMFRGLPSGPSAGAFLAFEVLPWLAVLAALMTTFLAVRHTRA
                     EEEEGTAELVSATPAGRLSPTVATLTHGLTANLVLGALTGLALTSTGLDPEGSWLTGA
                     ATAATGVVFVGIGLTAAQVVRTSRAASSLTIWVLVVTFLLRGVGNAAGTPQGGLLRTA
                     SAWPAWLSPFGWAEQTRPFDENLWWPVLLAAGCGIALGILAVLLQHARDMGAGFLAER
                     PGPAQARPALSSSTALVWRLAFGGIVGWAVGGALTGLLATTLGGVLGRVAGTNPAVVH
                     VITRLAGSGSLDEAAITVFFTMLGIVAGCCAVQTVVRARQEEVRGTAEPVLAAAVGRV
                     RWLADHLVVATGAVVIIVAVAVAAGCIGLAAGGHDASDLYRTVLVDGGGQLVAASVFT
                     AITGLILSLAPRVTVSLAWLLLILATMLGMFGPLLGMPGWTMNLSPFGVTPVVSGSGV
                     EARGLWWLLLAVAAGTSLCLAAMRRRQLAPAR"
     gene            3808..4293
                     /locus_tag="VFQ96_01635"
     CDS             3808..4293
                     /locus_tag="VFQ96_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256522.1"
                     /translation="MTHYRAVEAAEKGAAIMVAAGMPRMPARVMMALIAAPADGYTAA
                     DLRERLGVSAAAVSGAVRYLQQLHFIQRRSLPGRRRERYLLAPRAFYRSVVNNAPVYD
                     QSANALERMAADLGDDEAAGRRATQIAGFFRFLSRRMPQLIDEWESLQADDATALRPG
                     E"
     gene            complement(4458..4964)
                     /locus_tag="VFQ96_01640"
     CDS             complement(4458..4964)
                     /locus_tag="VFQ96_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256523.1"
                     /translation="MPDGSTVPYGFGDLLALARQSWVRELQAVLEGAGYADYRRSDAL
                     ALRLLVPGPLAVGRLGGALGVTRQAAKKVATGLAARGYAAILHDAHDGRQVNVVLTER
                     GQRYAQAIADARRRLDAGLARRLAPGLLAAADTVLRTALFDERARSLAARIPRPREGP
                     GGADATVT"
     gene            4985..5761
                     /locus_tag="VFQ96_01645"
     CDS             4985..5761
                     /locus_tag="VFQ96_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEU0256524.1"
                     /translation="MQPGYLVRRGLARAALGGIALYVVIDVALVFLRPEFSVLHNAES
                     DYGSAGGWAWLMDVNFVLRCLASLAAVGGLALAVPTGDTSSDITGGRATGRNAKHAGG
                     RDTDERVAGRRGGRLRVALALLVVWALASGLLALFPDDPAGTPLRATGRVHLALASTA
                     FLAALIGTILATAVLSRQPEWQGRRVSLLALSWGSIVPLTLVSRTGFRADSLGGLYEK
                     VFLAAQLAWLVVASVPVARGRAAPHSRPAAGEEPTARLSD"
     gene            5847..6371
                     /locus_tag="VFQ96_01650"
     CDS             5847..6371
                     /locus_tag="VFQ96_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0256525.1"
                     /translation="MEMKQPFESVVAQHGVTVLRVCRARLSIADADDAWSETFLAALR
                     AYPELPETANVEAWLVTIARHKVIDIRRAAERAPLPVAAVPEGTTGLGMPGTGDTVGD
                     DALWAAVGRLPAKQRRALTLRYLAGMSYREVAAALGGTAEAARRAAADGRKRLRDYYQ
                     DARSGAGSEKGKGR"
     gene            6368..6982
                     /locus_tag="VFQ96_01655"
     CDS             6368..6982
                     /locus_tag="VFQ96_01655"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0256526.1"
                     /translation="MNDDTPGGIAADALPTPIDPVDLSALHDRLETGAQAEGLLDIAY
                     TIVDSPVGRLLLAASPRGLLRVAYEREGHDLVLDSLTAVSPRILRAPKRLDEAARELE
                     AYFGGTRKAFDLRLDLSLSHGFRRLVQQHLPQIGYGTTLSYGQVARLVGNSKAVRAVG
                     TACATNPLPVVIPCHRVLRSDGTLGGYVGGAEAKATLLRLEAAA"
     gene            complement(6979..8544)
                     /locus_tag="VFQ96_01660"
     CDS             complement(6979..8544)
                     /locus_tag="VFQ96_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256527.1"
                     /translation="MMTTTSLAHPRAAVLLAPPKAPRRTRSPWVWRCLVAAIVVLALA
                     TRVVYSLRHSDVIDLQGYDSGVYYSAADSLVHGRLPYRNFLFVETPGIAVLVAPFALL
                     GTITHDSVGYAAANLFFTVIGAANAGMVATVLRRFGLSAAIFGGVFYAVTDVAVQSER
                     FIKLEPAATLLILIALVLLGDVRSPARRRHTLVAGILLGLACGFKIWYVVPVIVVTAA
                     LPGLRNRVRLFLAAAGSAAALMLPFFLAAPSAMFREVVVDQLGRRRTPVTVMGRLHEI
                     LGNPVVPHTVTRLLGLSVGEVTGGLGVLAAVATVFVLTRRSAGLYVWLLLSGMLVLFV
                     APSFFDYYTSLTSAPLALVLGVGFARLTALVSTRTGRLALTSSALVGLTLLNLPGQWH
                     AAPEPQPSIGLRRAVLAVPGCVVSDNPEILAATDVLSRDLVRGCVVWPDVTGWTYDAD
                     SGMRDGVYTPRSKNQRWQRHVTGYLTSGAAVIAYRPETELDARSRRVLASGPELFHDG
                     PWTLYATPRHTPA"
     gene            complement(8798..9124)
                     /locus_tag="VFQ96_01665"
     CDS             complement(8798..9124)
                     /locus_tag="VFQ96_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU0256528.1"
                     /translation="MTPLDAVDVDRVEEALSNVIDPELGIDIVNLGLIYALGWDEAGE
                     SLVIRMTLTSAGCPLSETIEEQVDDALDGVVPAHRIDWVWMPPWSPSLATDDGREMMV
                     SLGFTV"
     gene            complement(9295..>10586)
                     /locus_tag="VFQ96_01670"
     CDS             complement(9295..>10586)
                     /locus_tag="VFQ96_01670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0256529.1"
                     /translation="ESGTFDGIFIADVLGTYDVYGGNNVAAIRNGAQVPVNDPVLLIS
                     AMAAVTKNLGFGVTAGTAYEHPYPFARRMSTLDHLTKGRIGWNVVTGYLPSAARNMGH
                     DDQMEHDERYAYADEYLEVLYKLWEGSWEDDAVVEDRESGVFTDPSKVHEIGHRGKNF
                     TVPGIHLCEPSVQRTPVIYQAGASPRGVRFAAENAEAIFVAAPTKETLASTVTRIRDA
                     LEAAGRSRHAAKIYTLLTIITDATPEKARAKYEDYLQYVSEEGALTFMSGWMGVDLSK
                     FDPDEPVGNVKSNAIQSVLASFNESTEKEGVEWKVRDIATHAAIGGLGPFIVGSGEEI
                     ADQLQEIVDDTDVDGFNLAYALSPGTWEDVIRHVIPVLRERGAYPTEYVEGSLRHKLH
                     GRGDRLPAEHRGASYRVGGSRSTETGSVAQKAAAHAG"
BASE COUNT         1577 a         3745 c         3663 g         1601 t
ORIGIN      
        1 gggggtgcca aagacgccct gccggccccc ggcaaagcgg agaccggcac ggagctgtcg
       61 gccgcccaga tcgagacgat ccgtaaggcc aacaccttct tcatcgggac gtcgcacccc
      121 gacggcccgg cagacgcgtc acaccgcggc ggcgaacccg gcttcatcga ggtcgactcg
      181 ccgcacctgc ttcggtggcc tgactacaac ggcaacgcga tgttcatgac gctcggcaac
      241 atcagcctga atccgagcgt cggcctgctc tttcccgatt gggacacggg cgggctcctg
      301 caagtctcgg ggcgcgcgca gatcgactgg gatcccgcct cggcggcccg gctcgtgggt
      361 gcggagcgcg tcgtcgactt ccaggtggac gcggtgcaag agacccgaca tgccctgccg
      421 actcgctgga gcccggcccg gctcagccgc tataacccga agccggcgcc ggtggccgcc
      481 tgagcaccgg gcggtgtgcg accgcggtga gcaggtgcag gtgcttcggc ccgaacgggg
      541 tgccggcagg cccctcgacc tcgtcgatcc tcaccgcgtc gaggccgcct agcaacgcct
      601 cgatctgggt cctcccccga aaactcatgt cggtacggcc cacccacaag tcgcgcggcc
      661 cgaagaagtc ggcgatgagc cacgctcccg ggcgcaggca gtcgcgcacc gcctgccaca
      721 acgtcgcgaa cgactcctcc gggcagaatg gcagcgcgtg gccggcgtag acgaggtcgg
      781 cgtcaggcag cgaggcgaga tcctcgaagg ccgcacgcgt cgcggtgagg agcgcggcgg
      841 cggggcccga caccccggcc cgtacccgtg ccggggttcc cggcgcgccg tcgtaggcgt
      901 gcacgcgcca gccgcgggcg gctaagaacc gggcctccgt gccgtctccg cagccgaggt
      961 cgagggcgac gccggctggc aggtcgcccc gcctctcgag tacctggcgc acgagaggcc
     1021 tcggcgcgcg tcctgccgag gcggcgaaga acgacgtcca ctccaccctc tgactgtagc
     1081 ctccgcctct gcgaccgggt gaggccggcg gtgcctgcct tcggcggtcg tagcggcaat
     1141 ctcggcgccg cgctccccca cgcgaggctg cccgaggcca gttgcgaagc cgcttcacaa
     1201 atattcacaa ttttgtgaaa tttatgtaca ctggcgacca tgactgcggc catcgtcacg
     1261 cgcgacctgc acaagcacta cggtcgcctt cgggctctcg acgggctcga cctcgtcgtc
     1321 gacgccggcc aggtgcacgg ctttctcggg ccgaacggtg caggaaagac cacggcgatc
     1381 cgcacactgc tcggtttggc ccgcgccacg agcggggagg cccgggttct cgggatggac
     1441 ccgtggaagg acgccgtcgc gatccatcgg cgcctcgcct acgtgcccgg tgacgtgagc
     1501 gtgtggccga acctctccgg cggtgaggcc gtcgaccttg tggtgcggct tcgcggcacg
     1561 gctgtgaaag accgtcaata ccggctcgag cgggaacgcc tgatggacgc cttccgattc
     1621 gacccgcgca agaaaggccg cagctattca aagggcaacc ggcagaaggt cgcgctgatc
     1681 gctgcattcg cggtgcccgc agacctctac attctcgacg agcctacgag cggcctcgac
     1741 ccgctcatgt cggtggtgtt ccaccacgag gtgacgcggg tcgcggcggc aggggctacc
     1801 gtgttgctgt cgagccacat tctctccgag gtcgagcaac tctgcgaccg gttgtcgatc
     1861 atccgcaggg gccgggtcgt cgaggccggc acgctggcag acctgcgtca cctcacccgc
     1921 accgaggtgt cgttcgccgc cagagggata acgcccgacc ggccgcctgg cgtgcccggc
     1981 gcccacgacg tggtcgtggc ggggggccgg gtgcgcttca cgctcgacga cgaccgggtc
     2041 gccgaggcgt tgcccgcgct cgccgagctc ggtgtcgtgg ggctgcgggt gaccccgccg
     2101 tcgctcgagg agctcttctt gcgccattac ggcgacgatc cggccgctgc ccagacggcc
     2161 gagacgaggc ggcggtgaga aggttcgcgg cgctctggcg actccgggtg cgccgcgacc
     2221 ggctgcagct gcctctctgg atcgtcggca cggtgcttct ggcctatgcc ggctatgcgg
     2281 gggtgaccga gtcgtatggg agcctcgccg atcggcaggg catcctggcg gccgcccgcg
     2341 ccgaccccgt catcctgatg ttccgcgggc tgccctccgg gccgtccgcg ggcgcgttcc
     2401 tcgcgttcga ggtcttgccg tggctggccg tgctcgccgc actcatgacg acgttcctcg
     2461 ccgtgcggca cacccgtgcc gaagaggagg agggcacggc cgagttggtg tcggcgacgc
     2521 cggccggccg cctgtcgccg accgtcgcga cgttgacgca cggccttacg gcgaacctcg
     2581 tgctgggcgc cctcaccggc ctggcattga cgtccacggg gctagacccg gaaggctcct
     2641 ggctcacggg cgccgccacg gccgcgaccg gcgtggtctt cgtcgggatc ggcctcaccg
     2701 cggcgcaggt cgtgcgtacg tcgcgggcgg cctcctccct caccatctgg gtgctggtcg
     2761 tgacgttcct cctgcgcggc gtgggtaacg ccgccggcac gccccaaggc ggcctccttc
     2821 gcacggcaag cgcctggcct gcgtggctct cgccgttcgg gtgggccgaa cagacccgcc
     2881 cgttcgacga gaatctctgg tggcccgtgc tgctcgcagc cggctgcggt atcgccctcg
     2941 gaatcctcgc cgtcctgctg cagcatgcgc gcgacatggg cgccggcttt ctcgccgagc
     3001 ggccaggccc ggcgcaggcc cgacccgcgc tctcctcgtc aacggcactc gtctggcgtc
     3061 tcgccttcgg cgggatcgtc ggctgggcgg tcggcggcgc gctcacgggg ctcctcgcga
     3121 cgacgctcgg cggcgttctc ggccgggtgg ccggcaccaa cccggccgtg gtgcacgtca
     3181 tcacccggct cgccgggtcc ggctcgctgg acgaggcggc gatcaccgtg ttcttcacca
     3241 tgctgggcat cgtcgccgga tgctgtgccg ttcagacggt cgtccgggcc cggcaggagg
     3301 aggtgcgtgg cacggccgag cctgtgctcg cggcggccgt cggacgcgtc cggtggctcg
     3361 ccgaccatct cgtcgtcgcg accggcgccg tcgtgatcat cgtggcggtg gcggtcgccg
     3421 cggggtgcat cggcctcgcc gccggcgggc acgatgcgag cgacctctat cgcacggtgc
     3481 tcgtcgacgg cggtgggcag ctggtcgccg catccgtctt cacagcgatc accgggctca
     3541 tcctgagcct cgcaccacgg gtgacggtga gcctcgcatg gctgctgctc atcctcgcga
     3601 cgatgctggg catgttcggc ccgctcttag gcatgccggg gtggacgatg aatctgtcac
     3661 ccttcggcgt cacgcccgtg gtctccggca gcggcgtcga ggcgcgcggg ctgtggtggc
     3721 tgctcctcgc cgtcgccgcg ggcaccagcc tctgcctggc cgcgatgcgg cgccgccaac
     3781 tggccccggc cagataggtt cggtgtgatg acccattacc gcgcggtcga agccgccgag
     3841 aagggcgccg ccatcatggt ggccgcgggc atgccacgca tgcccgcccg cgtgatgatg
     3901 gccctcatcg cggcccccgc cgacggttac accgccgccg acctgcgcga gcgtctcggg
     3961 gtgagcgccg cggcggtctc cggcgccgtg cgttacctgc aacagctgca cttcatccag
     4021 cgccgttcgc tgccgggccg gcggcgagaa cgatatctct tggcgccgcg ggcgttttac
     4081 cgctccgtcg tcaacaacgc cccggtgtac gaccagtcgg cgaacgctct ggaacggatg
     4141 gccgccgacc ttggcgacga cgaggcggcc ggccggcgcg cgacccagat cgcggggttc
     4201 tttcgtttcc tctcgcgacg catgccgcag ctgatcgacg aatgggaatc gctgcaggcc
     4261 gacgatgcca ccgcccttcg gcccggcgaa tgaccccggg caggctcaca cgggcaccgt
     4321 ggcaccctgg cgagagcggc atccgcccga ttccttccgg cacctcgtcg aagcccgcgt
     4381 cgtcgagata tgccccaccg acacgtcggg caccgcgccc gcgcacctcg gcgcccggcc
     4441 tcgctaccgg tcctcgctca cgtcaccgtc gcgtcggcac cgcccgggcc ctccctcggc
     4501 cgcgggatcc gtgcggcgag ggagcgggcg cgctcgtcga agagcgccgt gcgtaacacc
     4561 gtgtcagccg ccgccagcag gcccggcgcc agccgtcgtg ccagccccgc gtcgagccta
     4621 cgccgggcgt cggcgatggc ctgggcgtag cgctgcccac gctcggtgag caccacgttg
     4681 acctgccggc cgtcgtgcgc gtcgtgcagg atcgccgcgt aaccgcgcgc cgcgaggccg
     4741 gtcgcgacct tcttggccgc ctgccgggtg acacccaggg ccccgcccag ccgaccgacg
     4801 gccagcgggc cgggcacgag caggcgcagc gccaaggcgt cgctacgccg gtagtcggcg
     4861 tagccggccc cttccagcac cgcctgcagt tcccgcaccc aactctggcg ggccagggcg
     4921 agcaggtcgc cgaatccata cggcacagtt gacccgtcgg gcatcagggc acacaatacc
     4981 aggtatgcaa ccaggttacc tagttcgtcg agggctggcg cgcgcggcgc tcggcggcat
     5041 cgcgctctac gtcgtgatcg acgtggcgct ggtctttctg cgaccggagt tctcggtgct
     5101 gcacaacgcc gagagcgact acggcagcgc cggcggctgg gcatggctca tggacgtgaa
     5161 cttcgtgctg cgttgccttg ccagcctcgc ggcggtcgga ggcctggcgc tcgccgtgcc
     5221 cacgggcgac acgagcagcg acatcacggg cgggcgggcg acgggccgca atgcgaagca
     5281 cgcagggggc cgcgacaccg acgagcgggt ggcgggtcgc cgtgggggcc ggctgcgagt
     5341 cgcactcgcc ctgctggtgg tgtgggccct cgcctcgggg cttctcgcgc tgttccccga
     5401 cgatcccgcc ggcacgccgc tgcgcgcgac cgggcgcgtc cacctggccc tcgcctccac
     5461 cgccttcctc gcggcgctca tcggcacgat cctcgcgacc gccgtgctca gccgccaacc
     5521 cgaatggcag ggacggcgcg tgtcccttct cgccttgtcg tggggctcga tcgtgccgct
     5581 caccctcgtg agccgcaccg gtttccgcgc ggactcgctg ggcggtctct acgagaaagt
     5641 attcctcgcc gcgcagctgg catggctcgt ggtggcctcc gtcccggtcg cccgcgggcg
     5701 ggcggcgccg cacagtcgcc cggccgcggg cgaggagccg acagcgcggc tgtcagacta
     5761 gacaggaaga cgacgggcgc cgtcggcatc gtggcccccc tttccgtaaa cctcacgttc
     5821 ggcgcatttc caccgtcttc aatgcgatgg agatgaagca accttttgag agtgtggtcg
     5881 cccaacacgg cgtgaccgtg ttacgggtct gtcgggcgcg cctcagcatt gccgacgccg
     5941 acgacgcgtg gtcggagacg ttcctcgccg cgctgcgggc gtatcccgag ctgcccgaga
     6001 cggcgaacgt cgaggcgtgg ctcgtgacca tcgcacgcca caaggtgatc gacatccggc
     6061 gtgccgcgga gcgtgccccc ctgccggtgg ccgccgtgcc ggaggggacg accgggctcg
     6121 gcatgcccgg caccggcgac actgtcggcg acgacgcctt gtgggcggcg gtcggccggc
     6181 tgcccgcgaa gcagcgacgc gccctgacgt tgcgctacct cgcggggatg tcgtaccgcg
     6241 aggtcgcggc ggccctcggt ggaacggcag aggcggcgcg cagggccgcg gccgacggcc
     6301 gcaaaaggct ccgagactac taccaagacg cccggtcggg cgcgggctcc gagaaaggaa
     6361 aaggacgatg aacgacgaca cgccaggcgg gatcgcggcc gacgcgctcc ccacgccgat
     6421 cgatcccgtc gatctttcgg ccctgcacga ccggctggag acgggggcac aggccgaggg
     6481 ccttctcgac atcgcctaca cgatcgtcga cagcccggtc gggcggctgc tgctcgcggc
     6541 gagcccacgg ggccttctcc gcgtcgccta tgagcgagaa ggccacgacc tggtcttaga
     6601 ttcgctcacc gcggtcagcc cacgcatcct gcgcgccccg aaacggctgg acgaggccgc
     6661 gcgcgaactc gaggcctact tcggaggcac gagaaaggcc ttcgacttgc gcctcgacct
     6721 ctcgctctcg cacggcttcc gccgtctcgt gcaacagcat ctgccgcaga tcggctacgg
     6781 gacgactctc agctacgggc aggtcgcccg acttgtcggc aactcgaagg ctgtgcgggc
     6841 cgtcggcacc gcgtgtgcca ccaacccgtt gcccgtggtg atcccctgcc accgggtgtt
     6901 gcgcagcgat ggcaccctcg gcggctacgt gggtggcgcg gaggcgaagg ccacactgct
     6961 acgcctggag gccgcagcct aggccggagt gtgccgcggt gtggcgtaaa gggtccatgg
     7021 gccgtcgtgg aagagctccg ggcccgaggc gagaacacgt cgagaacgag cgtcgagttc
     7081 cgtctctggg cggtaggcga taaccgccgc gccggaggtg aggtagccgg tgacatgcct
     7141 ctgccatctc tgattcttcg accgtggcgt atagaccccg tcccgcatgc cggaatcggc
     7201 gtcatacgtc cacccggtca catccggcca cacgacacat ccccgcacaa gatcacgaga
     7261 gagcacatcg gtggcagcca gaatctccgg gttgtcagag acgacacagc cgggcaccgc
     7321 aagaacggca cgacgcaacc cgatgctcgg ctgaggttcg ggggcggcgt gccactggcc
     7381 gggaaggttg agcagggtca gcccgacaag cgccgacgag gtcagcgcaa gcctgcctgt
     7441 cctcgtggac acgagcgcgg tgagccgcgc gaagccgaca ccgaggacga gcgccaacgg
     7501 tgcgctcgtc agcgacgtgt agtagtcgaa gaacgacggt gcgacgaaca gcacaagcat
     7561 tcccgacagc agaagccaga catagagccc ggccgagcgc cgggtgagca cgaagactgt
     7621 cgcgacggcg gcgagaaccc cgagcccgcc ggtgacctcc cccacgctga ggccgaggag
     7681 gcgcgtgacc gtatggggaa cgaccgggtt tcccagaatc tcgtggagtc gccccatcac
     7741 ggtgacagga gtgcggcggc gtcccaactg gtccacgacc acctcgcgga acatggcaga
     7801 cggcgccgcc agaaaaaacg gcagcatgag cgccgcagcg ctgccggccg ctgcgaggaa
     7861 cagtcgcacc cggttccgca ggccgggcag ggcggccgtc acgactatca ccggcaccac
     7921 ataccagatc ttgaacccac aggccaaacc caacaagatc ccggcgacga gggtgtggcg
     7981 ccgccgggcc ggtgaacgca cgtctcccaa tagcaccagg gcgatgagga tgaggagggt
     8041 cgccgccggc tccagtttga tgaagcgttc cgactgcact gcgacatcgg tcaccgcgta
     8101 gaacacgccg ccgaagatcg ccgccgacaa accgaagcgc cgcagcacgg ttgcaaccat
     8161 gccggcattg gccgcgccta tcactgtgaa gaacaggttc gccgccgcat accccaccga
     8221 atcatgtgtg atggtgccga ggagagcgaa cggcgccacc aagacagcga tccccggcgt
     8281 ctcgacgaag aggaagttgc ggtagggcag gcggccatgc acgagcgaat cagcggccga
     8341 gtagtagacg ccggagtcgt agccctgcaa gtcgatcaca tcgctgtggc gaaggctgta
     8401 gacgacccgg gtggcgagcg ccaagacgac gatcgcggcc acgaggcagc gccagaccca
     8461 cggtgagcgg gtacggcgtg gcgccttcgg gggtgcgagg aggaccgcgg cgcgggggtg
     8521 ggccagggat gtcgtcgtca tcatgccacc gcgtcgccgg tgttgcggat gccacaggca
     8581 tcgcgtatgg cgtcaccgga ggtgggcgca tcgcatggcg aaccggtcga ttcacgagcc
     8641 gctggaaaga ccacgactcg gagcgtatga gcgggcgccg cagaaacgat taagaatcgt
     8701 gccggtcggc accgcggtcg cgggcgggtg gcgagcgccg agcatgcgac cgccacccgt
     8761 tgctgtggct acatcgcacg atggcacggc atagcgctca caccgtgaag ccgaggctca
     8821 ccatcatctc gcggccgtcg tcggtggcca gggaggggct ccacggcggc atccacaccc
     8881 agtcgatccg atgcgccgga acgacaccgt ccagtgcatc gtcgacttgt tcctcgatcg
     8941 tctccgacag cgggcatccg gcactcgtga gcgtcatgcg gatgacgagg ctctcgcccg
     9001 cctcgtccca ccccaacgcg tatatcaggc caaggttcac gatgtcgatc cccagttcgg
     9061 gatcgatcac gttcgaaagc gcctcttcga cccggtcgac gtctacggcg tccagcggcg
     9121 tcatgccgcc gccaccgtcc gtgccccgcg gtgtcccctc atcactcatc agaccaccgt
     9181 atatgggtcg cgtgactcgt gacggccggc gccgcccgtt cacgtcaggc ccgtgggcag
     9241 cgctccctcg cggtggcgcc ggccgccgtt ctggggatca ccggagtgag ggtctcaccc
     9301 ggcgtgggcc gccgccttct gggccacgga accggtctcg gtggagcgcg atccgccgac
     9361 gcggtagctg gcgccgcgat gctccgcggg cagcctgtca ccccggccgt gcagcttgtg
     9421 ccgaagactg ccctcgacgt actcggtcgg gtaggcgccg cgctcgcgca gcaccgggat
     9481 gacatggcgg atgacgtctt cccaggttcc cggcgagagg gcgtaggcca ggttgaagcc
     9541 gtcaacgtcg gtgtcgtcga cgatctcttg cagctggtcg gcgatctcct cgcccgagcc
     9601 gacgatgaac ggcccgagcc cgccgatcgc ggcgtgcgtc gcgatgtcgc gcaccttcca
     9661 ctcgaccccc tccttctcgg tcgactcgtt gaagctcgcg agcaccgact ggatggcgtt
     9721 gctcttgacg ttgccgaccg gctcgtcggg gtcgaacttc gacaggtcga cgcccatcca
     9781 gcccgacatg aaggtcagag cgccctcctc gctgacgtac tgcaggtaat cctcgtactt
     9841 cgcccgggcc ttctcggggg tggcgtcggt gatgatcgtc aagagggtgt agatcttcgc
     9901 tgcgtggcgg cttctgcccg ccgcctccag cgcgtcccgg atgcgggtga cagtcgaggc
     9961 caaggtctcc ttcgtcggcg ccgccacgaa gatggcctcg gcgttctcgg cggcgaacct
    10021 cacgccgcgc ggcgacgctc ctgcctggta gatgacgggg gtgcgctgca cggagggctc
    10081 acacaggtgg atgcccggca cggtgaagtt cttgcctcga tgcccgatct cgtgcacctt
    10141 cgacgggtcg gtgaagacgc cgctctcgcg atcctcgacg accgcgtcgt cttcccacga
    10201 gccctcccac agtttgtaga gcacctcgag gtactcgtcg gcgtaggcgt aacgctcgtc
    10261 gtgctccatc tgatcgtcgt ggcccatgtt ccgcgccgcc gagggaaggt agccggtcac
    10321 cacgttccag ccgatgcgcc ccttggtgag atggtcgagc gtcgacatgc gccgggcgaa
    10381 cggatacgga tgctcgtagg cggtgcccgc ggtcacgccg aagccgagat tcttggtgac
    10441 ggccgccatg gcggagatga ggagcaccgg atcgttcacg ggcacctgcg caccgttgcg
    10501 aatggccgcg acgttgttgc cgccgtacac gtcgtacgtg ccgagcacgt cggcgatgaa
    10561 gatgccgtcg aaggttccgc tctcca
//
LOCUS       DASTNL010000049         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1151452, whole genome shotgun sequence.
ACCESSION   DASTNL010000049 DASTNL010000000
VERSION     DASTNL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151452"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFQ96_01675"
     CDS             <1..435
                     /locus_tag="VFQ96_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU0256530.1"
                     /translation="ARADGAYRLAFGSGDYRRDTGAENTALAMSYPRTRLVLASRISG
                     LPGPIDGPTVGSTHALLREQAADAVSLGMTGKLCLDGEQPAIINESMSPSPADVAWAI
                     DFLADFEARGSVIRDGSDKPRLARARTITRRAELFHIRPAGH"
     gene            648..1613
                     /locus_tag="VFQ96_01680"
     CDS             648..1613
                     /locus_tag="VFQ96_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0256531.1"
                     /translation="MIHARGLTRTFVTRHGKARKEIKAVAGVDIDVAEGEIVGFLGPN
                     GAGKTTTLRMLTTLLKPTSGTASVASFDLFRDAVKVRRSIGYVSQSGSTSRNALAGPE
                     IVDHGMLYGIGKAKATARGRELLDQLDLAGTWDRPTKTLSGGQRRRLDIAMGLVHDPR
                     LVFLDEPTTGLDPQARANLWTHISALRTVRGSTVFLTTHYLDEADALCDRILVIDHGV
                     IVAGDTPDRLKRQVSGDLVSLTLAAGSDVATAVATLSRAAEDAAQAEGLVVSARIRDG
                     GAALPALLRALDASGVTLDSIEVRRPTLDDVFLTMTGRSLREEAS"
     gene            1610..2365
                     /locus_tag="VFQ96_01685"
     CDS             1610..2365
                     /locus_tag="VFQ96_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256532.1"
                     /translation="MNFVHETGIIFRRQLRLSLREPAWIAIGVLQPVLYLALFGPLLK
                     PLASQLGTTNAYTFFVPGLLVQLAIFGALFVGFGLLGEYRDGVIEAERVTPASRMALL
                     LGRVLRDVLQVAVQSVILLVLGFVFGMRGSFWGMLLGFVLALILTGAISAGGYALALT
                     VKTEDSLAAVANGVSLPVLLLSGILLPMTLAPVWLQRVSDVIPVKHIVTGIRDLFTGG
                     AWGPTVAWAVFWAAVLIAAGLWIGTRTFRRQNA"
     gene            complement(2375..2800)
                     /locus_tag="VFQ96_01690"
     CDS             complement(2375..2800)
                     /locus_tag="VFQ96_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256533.1"
                     /translation="MTPHENANNGKNANDETPTGENANDQDVSDKKEGPARAEAVAAG
                     AEEPGVAGSGGGDAGRGAEPAASEPAPAPYDDYAERPVRVGTVVWGFLLVGIAAVFFT
                     ASQVDLSGVSPALIAVWVVLGIGAITITGGVTGALLRRR"
     gene            complement(2797..>3508)
                     /locus_tag="VFQ96_01695"
     CDS             complement(2797..>3508)
                     /locus_tag="VFQ96_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256534.1"
                     /translation="RRTRSNPLYSVIAIGLSLVAGATVTLLLGAGGWTPRAALAGVAV
                     TLGVCGLAVVVNGFLGRRSGGAGGVAILAVIALVLGSPFAWVAGPVVTGRSVSWSPSY
                     SPATVERTVVGGSARLDLTHYFDGGSSETAGSGSQAGTVILRVVGGPAEVIVPADASS
                     RVTARALGGSVTLDGRTSDRPFASERASFGPVTPGAAGRRVVVEVWVLGGEITATQAR
                     LPEGVTGSQTRQTKEPTT"
BASE COUNT          511 a         1283 c         1200 g          514 t
ORIGIN      
        1 gcgcgggccg acggcgccta ccggttggcg ttcggctcag gagactaccg acgcgacacg
       61 ggggcggaga acacggcgct cgcgatgtcg tatccgcgca cccggctcgt cttggccagc
      121 cggatcagcg gcctgccggg cccgatcgac gggccgaccg tcggcagcac gcacgcgctc
      181 ctgcgcgaac aggcggcgga tgcggtgagc ctcggcatga cgggcaagct gtgtctcgac
      241 ggggagcagc ccgccatcat caacgagtcg atgagcccct ccccggcaga cgtcgcgtgg
      301 gcgatcgact tcttggccga cttcgaggcg cgcggcagcg tcatccgcga cggcagcgac
      361 aagccgcgcc tcgcccgagc acgcaccatc acccgccgtg ccgagctctt ccacatccga
      421 cccgccggtc actgaacgcc cggcacccac agcgggttcg ccgacggcct cacacccgaa
      481 aggtcgcacg cccgagaagc cacacccggt tcgcactcag tctgcggccc ggcaggtctc
      541 gcacgccgcc accgggggct acggttgtcg ttcacggccg ttcacgcgaa cgttccgccc
      601 gacatcgcca ccacgagaca ccgagacacc gagagagagg cgtgttcatg atccatgccc
      661 ggggtctgac ccgcacgttc gtgacgcggc acggcaaggc gcgcaaggag atcaaggccg
      721 tcgccggggt ggacatcgac gtggcagaag gcgagatcgt cggctttctc ggccccaacg
      781 gcgcagggaa gaccacgacg ctgcgcatgc tcacgacttt gctcaaaccg acctccggca
      841 cggcgagcgt cgccagcttc gacctgttcc gcgacgccgt gaaggtgcgc cgctcgatcg
      901 gctacgtctc gcagagcggg tcgaccagcc ggaacgcgct cgccggcccc gagatcgtgg
      961 accacggcat gctctacggc atcggcaagg cgaaggcgac cgcgcgcggc cgggagcttc
     1021 tcgaccagct cgacctggcc ggaacatggg atcggccgac gaagacgctg agcggcgggc
     1081 agcggcgccg actcgacatc gccatggggc tcgtgcacga tcccaggctc gtcttcctcg
     1141 acgaaccgac gaccggcctc gacccgcagg ctcgcgccaa cctgtggacg catatctcgg
     1201 cgttgcgcac ggtgcgaggc agcaccgtct ttctcaccac gcattacctc gacgaggcgg
     1261 atgcgctctg cgaccgcatc ctcgtcatcg accacggcgt gatcgtggcc ggtgacacgc
     1321 ccgatcgcct gaagcgccaa gtctccggcg acctcgtgtc gttgaccctc gccgcgggct
     1381 ccgacgtcgc cacggccgtg gcgacgctga gccgcgcggc agaagacgcg gcacaggcgg
     1441 aggggctcgt cgtcagcgca cgcatccgcg acggcggcgc ggcgctgccg gccttgctgc
     1501 gcgcgctgga cgcttccggg gtcaccctcg actccatcga ggtgcgaagg cccaccctcg
     1561 acgacgtctt cctcacgatg acgggccgct cgctgcgaga ggaggcctca tgaacttcgt
     1621 gcacgagacc ggcatcatct tccgccgcca gctgcgactc tcgctgcggg agcccgcctg
     1681 gatcgcgatc ggcgtgctgc agccggtgct ctaccttgca ctcttcggcc cgctcctgaa
     1741 gccgttggcc tctcagctcg gcacgaccaa cgcatacacc ttcttcgtgc cggggctgct
     1801 cgtgcagctg gcgatcttcg gcgcgctctt cgtcggcttc gggctgctgg gcgaataccg
     1861 cgacggtgtc atcgaggccg agcgggtgac cccggccagc cggatggcgc tccttctcgg
     1921 ccgggtgctg cgcgacgtgt tgcaggtggc cgtgcagagc gtcatccttc tcgtgctcgg
     1981 cttcgtgttc ggcatgcgcg gctccttctg gggcatgctg ctcggcttcg tcctggcgct
     2041 gatcctcacc ggcgcgatct cggcgggcgg ctacgcgctg gccctcaccg tgaagacgga
     2101 ggattcgctc gccgccgtcg cgaacggggt gtctctgccg gtgctgctcc tgagcggcat
     2161 cctcctgccg atgaccctcg cgccggtatg gttgcagcgg gtttccgacg tcattccggt
     2221 gaaacacatc gtgaccggca tccgcgacct cttcaccggc ggagcctggg ggccgaccgt
     2281 cgcctgggcg gtcttctggg cggcggtgct gatagccgcc gggctgtgga tcggcacccg
     2341 caccttccgg cgccagaacg cgtagtcttc cccctcaccg ccggcgcagg agcgccccgg
     2401 tcacgccgcc cgtaatggtg atcgcgccga tgccgagcac cacccagacg gcgatgaggg
     2461 ccggggaaac accactcaga tccacctggg acgcggtgaa gaagacggcg gcgatgccga
     2521 cgagcaggaa gccccacacg acggtgccga cgcgcaccgg ccgttcggca tagtcgtcgt
     2581 atggcgccgg cgcgggctcg gaggcggcgg gttccgcgcc tcggccagca tcgcccccac
     2641 cggagccggc cacgccgggc tcctcggcgc cggcggcgac tgcctcggcc cttgccgggc
     2701 cctctttctt gtcgctcacg tcttggtcgt tcgcgttctc gccggtcggg gtttcgtcgt
     2761 tcgcattctt accgttgttc gcgttctcgt ggggggtcat gtcgtcggct ccttcgtctg
     2821 tctcgtctgc gatcccgtca cgccctcggg caaccgggcc tgggtcgccg tgatctcgcc
     2881 gccgagcacc cacacctcga cgacgacgcg gcggcctgcc gccccggggg tcacaggccc
     2941 gaacgatgcc cgctcggagg cgaacggccg gtcagaggtg cgcccgtcga gggtgaccga
     3001 gccgccgagc gcccgagccg tcacccggct ggaggcgtcg gcgggcacga tgacctcggc
     3061 cgggccgccg acgacccgca ggatcacggt gccggcctgc gatcccgacc ctgcggtctc
     3121 cgaagagccg ccgtcgaagt agtgggtgag gtcgagccgc gccgagccgc ccacgacggt
     3181 gcgttccacg gtggccggcg agtagctcgg cgaccacgac accgaacggc cggtgacgac
     3241 cggcccggca acccaggcga acggtgaacc gagcacgagg gcgatcactg cgaggattgc
     3301 gacgcccccc gcaccgccgc tgcgtctgcc gaggaacccg ttgacgacga ccgcgagccc
     3361 gcagacgccg agcgtgacgg cgacaccggc aagcgccgcc cgcggcgtcc agccgcccgc
     3421 gccgaggagc agcgtgacgg tcgcaccggc gacgagcgag agcccgatcg cgatgacgga
     3481 gtacagcggg ttggagcggg tgcgtcgg
//
LOCUS       DASTNL010000050         7329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_39555, whole genome shotgun sequence.
ACCESSION   DASTNL010000050 DASTNL010000000
VERSION     DASTNL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39555"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1298)
                     /locus_tag="VFQ96_01700"
     CDS             complement(27..1298)
                     /locus_tag="VFQ96_01700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0256535.1"
                     /translation="MNDSSLPTLERYCDVVVIGGGPAGLAAALETGRRRRSVIVIDAE
                     DPALTDGTGTADDDALAAAREKVRGWGGEIITGWVERVTHTDDDLFRAELAGGGRVVA
                     RRVVIAPEGPADVLPEVESAHPEAKGTGPAPRLPEQGMPAPEKAASSTKIPGVYAIAP
                     ATRAEGWAALAAEGAQAGIAAASSLADEDARAGSAPRPSGNEADWDHRYSGDPLWSGN
                     PNGALVAEAGEIAPGSALDVGAGEGGDALWLAEHGWRVTASDISRRALERVEAEAQRR
                     GLCVACHHADANAPGAFPHEAFDLVCASYASIPRTPDDRAVHGIVGSVAPGGTLLVIG
                     HDLRPAEEHADTATPCVAGHPDLTAGLEHAGHGTSFDPEAYVRVDDFAAALAGRADWV
                     VEVHEARPRPAGAASASHHTSDLVFRARRRA"
     gene            complement(1550..2809)
                     /locus_tag="VFQ96_01705"
     CDS             complement(1550..2809)
                     /locus_tag="VFQ96_01705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEU0256536.1"
                     /translation="MVTRQIPNPSEIFELMKFKKPTLDFKKRRLENALTIEDLRRIAK
                     RRTPAAAFDYTDGAAEGEISITRARQAFRDIEFRPGILRGTDKVDTTRQIFGGPSALP
                     FGIAPTGFTRLMQTEGETAGASAAGAAGIPFSLSTLGTTSIEDVKKANPAGRNWFQLY
                     VMRDREISYDLVRRAAAAGFDTLFFTVDTPVAGARLRDKRNGFSIPPQLTAKTILNAI
                     PRPWWWWDFLTTPPLEFASLTSTGGTVGELLDAAMDPTISFDDLKTIRGLFPGKLVVK
                     GVQNLPDAKRLVDLGVDGLVLSNHGGRQLDRAPVPFRLLPEVVREVGRDVDVAMDTGI
                     MSGADIVAAIAMGARFTLVGRAYLYGLMAGGRAGVDRAIQILRDGVIRTMKLLGVATL
                     DELTPDHVVQLTRLSPVRRAQQETTRV"
     gene            complement(2973..3776)
                     /locus_tag="VFQ96_01710"
     CDS             complement(2973..3776)
                     /locus_tag="VFQ96_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0256537.1"
                     /translation="MPRTTTSPSGDGHTATDQRPGARAWQSVLTTIEGRLLTGELAPG
                     DHLPPERTLAAELAVGRSSVREALRVLEALGLIRTATGSGPSAGAIIVAAPRGGMSAL
                     LRLQVAAQGFPVHDIVHTRLLLETGIVEELARTTPRPALASCRSLLTAMSDQALPSGD
                     FLALDTRFHVALAEATHNTVVTAMMAGLRESIESYVFAGSAALPDWGRTAARLRCEHQ
                     AVIDAIEQGESAKARTRIRDHITGYYAEISAPSAAAATDEGPRPPAPAR"
     gene            4545..4685
                     /locus_tag="VFQ96_01715"
     CDS             4545..4685
                     /locus_tag="VFQ96_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256538.1"
                     /translation="MAVTPVATVLLFFLTGLMFDFRWAWLWFLAVPVMGAIIYAPWYE
                     EE"
     gene            complement(4695..5579)
                     /locus_tag="VFQ96_01720"
     CDS             complement(4695..5579)
                     /locus_tag="VFQ96_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0256539.1"
                     /translation="MQRIVPNVWCQGTAAEAGEYYAATFPDTSWEVTASYPTEDLPDF
                     QRSLSGQPLVVDVAIEGYQIRLINAGDELRPTPAVSFIVNVDPPSFEGGEDEARSVIH
                     ELWEALADGGEERMPLDEYPYSKLYGWVEDRYGVNWQLMLTDPNGEPRPRIVPQLFFA
                     GDVAQAREAIDFYTGLFPDSRIGTLAPSPDDAARVLYGDFTLAGQWFSAMDAGPGHDI
                     TFTPGVSLQADCTDQTEIDRLWHALSAVPEAEQCGWLTDRFGVSWQIVPDSMADLMQH
                     PGAYQRMLAMKKIVIDEL"
     gene            5904..6329
                     /locus_tag="VFQ96_01725"
     CDS             5904..6329
                     /locus_tag="VFQ96_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0256540.1"
                     /translation="MATQLNPYLSFRDTARQAMEFYHTVFGGELTLSTFGEFHASEDP
                     AEADLIMHGALTTSGGLTLMASDTPKGMEYTAGNCSVSLTGQDEAELRRYWDGLSTGA
                     KVGMPLEKAMWGDVFGMCTDKFGVTWLVNISAAPGEAAG"
     gene            6597..6941
                     /locus_tag="VFQ96_01730"
     CDS             6597..6941
                     /locus_tag="VFQ96_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEU0256541.1"
                     /translation="MDSQAAVIRVTTLVMRPGQRTGLLEAAAENATAARRAPGCLSAE
                     VCEQRGEEGRFLVVSRWSSRKALDKFLEWHKSVAHASLADFTLGKPTSLHHEVVAGGE
                     RARTGGLNARPA"
     gene            complement(7090..>7329)
                     /locus_tag="VFQ96_01735"
     CDS             complement(7090..>7329)
                     /locus_tag="VFQ96_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256542.1"
                     /translation="LLPDVFYAPRGLTTIVLMLGIPAALRMPSFSQGVLFFVVMLSTI
                     VMTVGSIFFTPKSARREQPEDGPAETLPTAAARRE"
BASE COUNT         1022 a         2447 c         2683 g         1177 t
ORIGIN      
        1 gtgtcgcccg cccgcgccgc cccgtctcag gcgcggcgcc gggcgcggaa cacgaggtcg
       61 ctcgtgtgat gcgaggcgga ggccgccccg gcgggccgtg ggcgggcctc atggacctcg
      121 accacccagt cggcccggcc cgcgagcgcc gccgcgaaat cgtcgacacg cacgtaggcc
      181 tccgggtcga acgacgtgcc atggcccgcg tgttccaggc cagctgtcag gtcgggatgc
      241 ccggccacgc agggcgtggc cgtatcggcg tgctcctcgg ccggcctgag gtcgtggccg
      301 atcacgagaa gcgtgccccc gggggcgacg gagccgacga tgccgtgcac ggcccggtcg
      361 tcgggcgtgc gggggatcga cgcgtagctg gcacagacga ggtcgaaggc ctcgtgtggg
      421 aacgcgcccg gggcgttggc gtcggcatgg tggcaggcga cgcacaggcc acggcgttgc
      481 gcctcggcct cgacgcgttc cagggcgcgg cgggagatgt cgctggccgt cactcgccag
      541 ccgtgctccg ccagccagag cgcgtcgccg ccctcgccgg cgccgacgtc gagggcactg
      601 ccgggagcga tttcgccggc ctcggcgacc agggcgccgt tcgggttgcc gctccacagc
      661 gggtcgccgc tgtagcggtg gtcccagtcg gcttcgttgc ccgaggggcg cggggccgaa
      721 cctgcccggg catcctcgtc ggcgaggctt gacgccgccg ctatgccggc ctgggcgccc
      781 tccgcggcca gggctgccca cccctccgcc cgggtggcag gcgcaatcgc gtagacgcca
      841 gggatcttgg tggagctggc cgccttctcg ggcgcgggca tgccctgttc gggcaggcgg
      901 ggcgccggcc cggtgccctt ggcctcaggg tgcgcgctct cgacctccgg gagcacgtcg
      961 gcaggcccct cgggtgcgat gacgacccga cgtgccacga ctcggccacc gccggccagt
     1021 tcggcgcgaa agaggtcgtc gtcggtgtgc gtcacgcgct ccacccaccc ggtgatgatc
     1081 tcgccgcccc agccgcggac cttctcgcgc gcagccgcga gcgcatcgtc gtctgcggtc
     1141 ccggtcccgt ccgtcagggc cggatcctcg gcgtcgatca cgatcaccga acgtcggcgg
     1201 cgcccggttt cgagtgcggc ggcaaggccc gcggggccgc ctccgatgac cacgacgtcg
     1261 caataacgct caagtgtcgg gaggctggaa tcgttcatga agacgacgct accgaccccg
     1321 atgcgcgacg tgggcttatt cgcggctcgc ggctcggtgc ccgcccgggt atgtgtcgag
     1381 tcggcgcatg acgctgcctc cctccgtggg tttcgtcacc tgcgggccag tcacgcccgc
     1441 gcgctgcctg cccgaggtca gcaccacgaa tgcacataca ggaccagacg gcatcggtca
     1501 cgcctgcgcg ctgcccgagg tcagcatgag gcagctacgg ttccacccct cagacgcgcg
     1561 tcgtctcttg ctgggcgcgc cgtaccgggg agagccgcgt gagctgcacg acgtggtcgg
     1621 gggtgagctc gtcgagcgtc gcgacgccca acagcttcat ggtgcgaatg acgccgtcgc
     1681 gcagaatctg gatcgcacga tccacgcccg cccggccgcc ggccatgagc ccgtagaggt
     1741 aggcgcggcc gacgagcgtg aagcgggcgc ccatcgcgat cgcggcgacg atgtcggcgc
     1801 ccgacatgat gccggtgtcc atcgccacgt ccacgtcacg gccgacctcg cgcacgacct
     1861 ccgggaggag gcggaacggc acgggagccc ggtcgagctg gcggccgccg tggttggaga
     1921 gaaccaggcc gtcgacgccg aggtcgacga gcctcttcgc gtcgggcagg ttctgcacgc
     1981 ccttgacgac gagcttgccg gggaagaggc cgcggatggt cttcaggtcg tcgaagctga
     2041 tcgtcgggtc catcgccgcg tcgagaagtt ccccgaccgt gcccccggtg gaggtgagcg
     2101 aggcgaactc gagcggcggt gtcgtcagaa agtcccacca ccaccagggg cgtgggatcg
     2161 cgttcaggat cgttttggcc gtcagttggg gcggaatgga gaagccgttg cgtttgtcgc
     2221 gcagccgggc gcccgcgacg ggcgtgtcca ccgtgaagaa gagcgtgtcg aagccggccg
     2281 ccgccgcgcg ccgcacgagg tcgtaagaga tctcgcgatc gcgcatcaca tacagctgga
     2341 accagtttcg gccggccggg ttcgccttct tgacgtcttc gatcgaggtg gtgccgaggg
     2401 tcgacagcga gaaggggatg ccggccgcgc cagccgccga ggcgcctgcc gtctcaccct
     2461 cggtctgcat gaggcgtgtg aacccggtgg gtgcgatgcc gaacggcagc gcggaggggc
     2521 cgccgaagat ctgcctggtc gtgtcgacct tgtcggtgcc gcgcaggata cctggccgga
     2581 actcgatgtc gcgaaacgcc tgcctcgccc tcgtgatcga gatctcgccc tccgcggcgc
     2641 cgtccgtgta gtcgaaggcg gccgccggcg tgcggcgttt ggcgatgcga cgcaggtctt
     2701 cgatggtcag cgcgttctcc agccggcgct tcttgaagtc gagggtcggc ttcttgaatt
     2761 tcatcagctc gaagatctcc gacgggttgg gaatctgtct ggtgaccatc gtggtccttt
     2821 ctctggaggg gtctcggttg cttgtttggc ggtgtgcgga tcgggtcgga ggcgccatca
     2881 cggcgccgcg ccccacggtc tgttcccggc cctctccgcg gtgttcatcg catgatctcg
     2941 gtgctgccgc gcgccgcggc cggttggtgt gttcatcgcg ccggagcggg aggccggggg
     3001 ccctcgtcgg tcgcggcggc agccgagggg gcggagatct cggcgtagta gccggtgatg
     3061 tggtcgcgga tgcgcgtgcg cgccttcgcg ctctctcctt gctcgatcgc gtcgatgact
     3121 gcctggtgct cgcaccgcag gcgcgccgcc gtccggcccc agtcgggcag cgcggctgag
     3181 ccggcgaaga cgtacgactc gatcgactcc cgcagcccgg ccatcatcgc ggtcacaacc
     3241 gtgttgtgcg tagcctcggc cagggccacg tgaaagcggg tgtcgagggc gagaaagtcg
     3301 ccggagggca gcgcctggtc gctcatcgcg gtgagaagag agcggcagga ggcgagggcc
     3361 gggcgcggtg tggtgcgggc gagctcttcg acgatgccgg tctccaggag caggcgggtg
     3421 tgcacgatgt cgtgcacggg gaaaccctgc gccgcgacct gaaggcgcag gagggcgctc
     3481 atgccgccgc gaggggcggc gacgatgatc gcgccggcgg aaggcccgct gccggtggcg
     3541 gtgcggatga gaccgagcgc ctctaagacg cgcaacgcct cccgtacgct ggagcggccg
     3601 acggccagct ccgcggcaag ggtgcgctcg ggcggcaggt ggtcgccggg cgcgagctcg
     3661 cccgtcagca gccgcccctc gatcgtcgtg agcacgcttt gccacgcccg tgcgcccggc
     3721 cgttggtcgg tggcggtgtg cccgtcaccg cttggcgacg tcgtggtcct cggcatcctg
     3781 gcaccctttg ctttgtggtc agaccacatg ataatcgggt gcctcgcgct tgtccatcgc
     3841 gggcacatag cgaggtgggg caccatggag agatggtcga ttaccggaac cctgaccgac
     3901 ccgaagaacg gctgagtcat gcggagcgtg acgccgcggt cgcgaccctg cgccgccatg
     3961 cgatcgacgg ccgaatcacc gcggctgaat tcgaggaacg ttcgcgcatc gtctcttccg
     4021 cacgcactcg cgacgacctg atgggaccgt tcgcagacct tcccaccctc cccgacctgc
     4081 cgtgggaggc gcacggcacg gccgccgtcg atcccggcag ccgcgggggt gaggcgtggc
     4141 gctcatcccg ggcgccggag ggggtcgcgg agcctgcgtc ggacgcgccg gctccgtcct
     4201 cctcggccgt gccacagcgg cccgcccggg agagacgcgt ggactcctcg tccgcggtgg
     4261 aggggtcctc cccagagagg cgtgcgccgc gggtgccggc gcaccccgct ccagacccgg
     4321 cggagcgtct gccgggagga gtagcaccgc agccagagga tgggcgcacg tcggacctgt
     4381 cggctccgcc tttcgtgtca cgtccggtgg gcggtgggca gcaggggtcg gccgcggtgc
     4441 cgcaggagcc gcacggtgag cccgctcctt cgcgccgccc gcggggcagg cggctacgtc
     4501 gtggcggggt ccggcccttg ggcggcagga tcggagccac ggtcatggcc gtcacgccgg
     4561 tcgcgacggt tctactcttc tttctgaccg ggctgatgtt tgatttccgg tgggcgtggc
     4621 tctggttcct cgcggtgccg gtcatgggcg ccatcatcta cgcgccctgg tacgaggagg
     4681 agtgaggggc cgtctcagag ctcgtcgatg acgatcttct tcatggccag catccgttga
     4741 tatgcgccgg ggtgttgcat gaggtcggcc atgctgtcgg gcacgatctg ccagctcacg
     4801 ccgaaccggt ctgtcagcca gccgcactgc tcggcttcgg gcacggcgga gagggcgtgc
     4861 cacagccggt cgatttccgt ctggtcggtg cagtcggcct gcagggagac tcccggcgtg
     4921 aacgtgatgt cgtggcccgg gccggcatcc atcgccgaaa accattggcc ggccagcgtg
     4981 aagtcgccgt agaggacgcg tgcggcatcg tcggggcttg gcgcgagggt gccgatgcgc
     5041 gagtcgggga acaggcctgt gtagaagtcg atcgcctccc tggcctgggc gacgtcgccc
     5101 gcgaagaaga gctggggcac gatcctcggg cgcggctctc cgttcgggtc ggtgagcatc
     5161 agctgccaat tcacgccgta tcggtcttcc acccagccgt agagcttgct gtacgggtat
     5221 tcgtctagag gcatacgttc ctcgccgccg tcggcgaggg cctcccacag ctcgtggatc
     5281 acggagcggg cctcgtcttc gccaccctcg aaggacggcg gatccacgtt cacgatgaac
     5341 gacacggccg gcgtcggccg taactcgtct ccggcgttga tcaaccgaat ctggtagccc
     5401 tcgatggcga cgtcgacgac cagcggttgg cctgacaggc tgcgttgaaa gtcgggtaga
     5461 tcctccgtcg gatacgacgc ggtcacctcc cacgatgtgt cggggaacgt cgcggcatag
     5521 tactcgccgg cttcggccgc ggtgccttgg caccacacgt tcggcacgat cctttgcatg
     5581 attcctcccc cggtaaagct gcaaacgcta gcacgccggc gtgtcgcaca caatggattt
     5641 cgcgccgctc gccgagagcg aatgagaggc gccgagagcc gttctcggcc ggagcgcggc
     5701 cggactcttc tcggtcggcc acggcacggt cgaggtgacc cggtgggcac gtcgtgggcg
     5761 cgtcggcgaa gccgtcatcg tgggcgtgtt gcggtgcgac gtgcccctcg cgtggagcgg
     5821 cggatgggct cataatctac gcaacgcgcc cggcgacgcc accctggtgc tgccgtggcc
     5881 gcgtcaaggg ggggcatgac cagatggcta cgcagctcaa cccatacctc agctttcgcg
     5941 atacggcgcg gcaggcgatg gagttctatc acacggtgtt cggcggcgag ctcacgctga
     6001 gcacgttcgg agagttccat gcctccgaag acccggccga ggccgatctg atcatgcatg
     6061 gcgcgctgac gacgagcgga gggctgacct tgatggcctc tgatacgccg aaaggcatgg
     6121 aatataccgc cggcaactgc tcggtctcgc tcaccgggca ggacgaggcc gagctacgcc
     6181 gctattggga tggcctgagc accggagcga aggtcggcat gccgttggag aaggcgatgt
     6241 ggggcgacgt gttcggcatg tgcaccgaca agttcggcgt cacctggctc gtgaacatct
     6301 ctgccgcgcc cggggaggcc gcggggtagg tcggggccgc ccggggcatc cggtgtcggc
     6361 cgccgatacc gatacggccg cggggtagcc ttcggccgac ctggccctgg ccccggggct
     6421 ctgccggcgg ggcgaaagca tgccggatcg gccgtctggc cgtcgggcgc ggcgagtccc
     6481 gcccgcgagg ggcgaaagag cccgctttcg gcgtcggtga gactgctcgt cggcgaggct
     6541 gctcgagggc tggtaatttc cggcgtcggc cgctccctgg gctgcaaact ggtgtcatgg
     6601 actctcaggc ggccgttatt cgtgtcacga cgctcgttat gcggcccggc cagcgtaccg
     6661 gccttctcga ggcagcggcg gagaacgcca cggccgcgcg gcgggcgccc gggtgcttga
     6721 gcgccgaggt gtgcgaacag cggggcgagg agggccgatt tctcgtcgtc tcccgttggt
     6781 cgtcgcgaaa agccctcgat aaattcctcg aatggcataa aagcgtcgca cacgcgagcc
     6841 tcgccgactt caccctcgga aagcccacat ccttgcacca cgaggtcgtc gctgggggcg
     6901 agcgtgcgcg tacggggggc ttgaacgccc gccccgcgtg aggggccagc ggcggaggcc
     6961 tgcgacgccg cccggcgagc gcggaccccc ggcgggcgtt cccgagctct ccccagcgcg
     7021 ccgtcgtgga ttctgggggt gcttccgcgc gttccctggc tgctgtgagt cttagccgac
     7081 gctctcgcgc tattcccgac gagccgcggc ggtcggcaac gtctccgcag ggccgtcctc
     7141 tggttgttcg cgcctcgcag acttcggtgt gaagaagatg ctgccgaccg tcatgacgat
     7201 tgtcgagagc atgacgacga agaagagcac gccctggctg aagctcggca tgcgcaatgc
     7261 ggccgggata cccagcatca agacgatcgt cgtcaggccc cggggcgcgt agaacacgtc
     7321 gggcaaaag
//
LOCUS       DASTNL010000051         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_225841, whole genome shotgun sequence.
ACCESSION   DASTNL010000051 DASTNL010000000
VERSION     DASTNL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225841"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFQ96_01740"
     CDS             complement(<1..426)
                     /locus_tag="VFQ96_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0256543.1"
                     /translation="MRDPDDTIAQFGLGADDVAAWHASGLHGDRLDDWLTDRVARRPA
                     GERARAVYGADDVHDFARRAVLDALALEPGERLLELGCGGGLLLREAVEAGVVATGLD
                     HSEEMVGLARERAPGAEVVVGRAEQLPFEKATFDAVAMSV"
     gene            567..1145
                     /locus_tag="VFQ96_01745"
     CDS             567..1145
                     /locus_tag="VFQ96_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256544.1"
                     /translation="MKAKISAAIAVAACVYAAAAWGAATDPRPFVLRPADFPSGFQRN
                     APHYVSKRLAAKGVPPKTFARFGYVNGYSVVYNKTGTALLGILRAQSNATVYQAPGGA
                     HAAMRYVEANVESTKKFPTHRLAVGVPLGNEARIYKVTEKSSKLSADVVVVLWRSGRV
                     LSTVTASGISGTVDPAQVEILALRQQRHISGK"
     gene            1328..1747
                     /locus_tag="VFQ96_01750"
     CDS             1328..1747
                     /locus_tag="VFQ96_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256545.1"
                     /translation="MKLKTLTGVSMAALALASIAIAAPPSGKGKPPKTGPGCKPMVSV
                     VLTGKLAADGAAAPSTLSVTVTGGNRFARAYKKGTQPVPVALTTSTKVNRSGDHMAAD
                     LKSGDRVNIHARSCAADLANGATPALTATRVVAHPSA"
     gene            2028..>2219
                     /locus_tag="VFQ96_01755"
     CDS             2028..>2219
                     /locus_tag="VFQ96_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU0256546.1"
                     /translation="MERRNMVLGGRDSVKVAIAQVSPVYLDRDRSIERACETIREAAA
                     GGAELIVFPEVWLAGYPYWT"
BASE COUNT          350 a          797 c          768 g          304 t
ORIGIN      
        1 gacggacatc gcgaccgcgt cgaaggtcgc cttctcgaac ggcagctgct ccgcgcggcc
       61 gacgacgacc tcggcgcccg gcgcgcgctc acgcgcgagc ccgaccatct cctcgctgtg
      121 gtcgagaccg gtcgcgacga cgccggcttc gaccgcctcg cggagcagca gcccgccgcc
      181 gcagccgagc tcgagcagac gctcgcccgg ctcgagagca agcgcatcga ggacggcgcg
      241 ccgtgcgaag tcgtggacgt cgtcggcgcc gtacacggcc cgggcgcgct ccccggccgg
      301 ccgccgggcg acgcggtcgg tcagccagtc gtcgaggcga tcgccgtgga gcccgctcgc
      361 gtgccaggca gcgacatcgt cggctccgag gccgaactgc gcgatggtgt cgtcggggtc
      421 cctcatcagg cgaaggcgca cggccggtcg ggctcgcgcc tttcagcgag cggcgaggag
      481 caccggtcga ccctagacga ggcgctagcc tgccgctgac gaaagctgct cttgacgggt
      541 tgtcggcaac agcaaaggat gcgacggtga aggcgaagat ctcggcggcg atcgccgtgg
      601 cggcgtgcgt gtacgcggct gcggcatggg gtgctgcgac cgacccgagg ccgttcgtgt
      661 tgcggcccgc cgatttcccg agcggtttcc agcgcaatgc tccgcactac gtctcgaagc
      721 ggctcgccgc gaaaggcgtc ccgccgaaga cgtttgcccg gttcggctac gtcaacggct
      781 attcggtcgt ctacaacaag accgggacgg ccctgctcgg catcctcagg gcgcagtcga
      841 atgcgaccgt ctaccaggcc cctggcggag ctcatgcagc catgcggtac gtggaggcca
      901 atgtcgagtc cacgaagaag ttcccgacgc accggctcgc cgtgggcgtt ccgctcggca
      961 acgaggcccg catctacaag gtcacggaga agtcgtcgaa gctctcggcc gacgtcgtcg
     1021 tcgtcctgtg gcggtcggga cgggtcctct ccacggtgac ggcgtccggc atctcgggca
     1081 cggtcgaccc cgcccaggtc gagatcctcg ccctgcggca acagcggcac atcagcggca
     1141 agtaggcggc tcgcgccctg atccgaccct cgccggaggg tcggacgccg gccggtcgcg
     1201 ctcgaatcac ccgttcgagg ttcgccgcct tacacggctc ttacgttcgc atgacccgct
     1261 gcttgcgcgg ccggaaacga tccgagacga ccgctcgaca cggactaccc gaaggagacc
     1321 cgactccatg aagctgaaga cactgaccgg cgtttcgatg gcagccctcg ccctcgcctc
     1381 gatcgcgatc gccgccccgc cctcgggcaa gggcaagccg cccaagaccg ggccagggtg
     1441 caagccgatg gtcagcgtgg tgctgaccgg caagctcgcg gccgacggag cggcagcccc
     1501 ctcgacgctg tcggtcacgg tgaccggcgg caaccggttc gcccgggcct acaagaaggg
     1561 gacccagcct gtcccggtcg cgctgaccac gtcgaccaag gtcaaccgga gcggcgacca
     1621 catggccgcc gacctcaagt ccggcgaccg ggtgaacatc cacgcgcgca gctgcgccgc
     1681 cgacctggcc aacggggcca cgccggcact gacggcgact cgcgtggtcg cccacccctc
     1741 ggcataggtt tttcggccca gccagggact gcggggggcg gccgaagccg cccccccgca
     1801 gcgtctcgag gttccgcatc gtgtcgatcc gcaagggcgc tccgacagaa gccggccggc
     1861 acgtcggtcg gcgacgatcg ctccgaccgg atcatcgccg gcgcgtcccg ttcagggaac
     1921 cacgtcatgt cgacccgccg caaggagtac gcgatcgggt gacaactgcg cccttccccg
     1981 acccgtaggc tcgccgcgag atctacgacc cgacggaggt agccgcgatg gagaggcgga
     2041 acatggtgct cggcggccgc gacagcgtca aggtggcgat cgcgcaggtc tcgcccgtct
     2101 acctcgaccg tgaccggtcg atcgagcgag cgtgcgagac gattcgggag gcggcggcgg
     2161 ggggagccga gctcatcgtc ttccccgagg tctggctcgc ggggtacccg tactggacc
//
LOCUS       DASTNL010000052         6804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_595751, whole genome shotgun sequence.
ACCESSION   DASTNL010000052 DASTNL010000000
VERSION     DASTNL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595751"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFQ96_01760"
     CDS             complement(<1..315)
                     /locus_tag="VFQ96_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256547.1"
                     /translation="MAAADEVGPSRLLLEHIELDGDVEEGTGLVLDAEALFFGEEFVT
                     EPPPLPFATDFGNISHLVPAALIGVGRPGGWGFHTARGAAEFSSPDALRSALTIARVL
                     ALS"
     gene            969..2117
                     /locus_tag="VFQ96_01765"
     CDS             969..2117
                     /locus_tag="VFQ96_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0256548.1"
                     /translation="MTTSHRRHAEVAGAGFAGLTMATALAQRGWSVRVHERGKKLREE
                     GAGILLWQNSLAVLDALGVTDTLMTRSMTPPFYETRMQNRTVSAEALEGIRWRTMTRP
                     FLHKTLLDAARAAGAEVIPGSEVASATDDGVMTLRDGTRLEADLVVGADGVGSNVRDS
                     LGFRYDRQRSRDGISRFLVGRRKADLAAIEPETDWDNMIDFWNLEPRVLRVLYTPVND
                     KELYLALMAPAADEAGTRVPIDLDLWTSIHPQLAPVLEEVARKEGKHYGYQTTRLDEW
                     TRGRVALVGDSAHAMCPALAQGAGCSMMNAYTLAQVVSDNPLGDLPDAIREWERLERP
                     YTDRAQTRSQGYADTRDMSKGNQFTGEVNETTLYDPTDPHRHDAVRAG"
     gene            2240..2833
                     /locus_tag="VFQ96_01770"
     CDS             2240..2833
                     /locus_tag="VFQ96_01770"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEU0256549.1"
                     /translation="MSANEIEESYEVREWFGFVQEIRHEGGTPPDSPLVKAAAGVVFR
                     NPFAGRWQDDLAALTGPSAALGTELGRRAVALLAGLPVEGYGKGGIAGLDGEQEHVVA
                     CVTTVFGNAFRAAVGGGRAWISSASKTAAAGSSIDIPLAFKDEIYVRSHYDAVTVQVP
                     DGPRADELLIVVAVSSGGRIHQRVGGLSRDEALARLA"
     gene            2909..3670
                     /locus_tag="VFQ96_01775"
     CDS             2909..3670
                     /locus_tag="VFQ96_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0256550.1"
                     /translation="MSYTSGQYQQDESIANMLTDVARAHRILELEGHGDMSMGHLSLR
                     DPFGRGLWLKRGNLALSEVVGDDFILIDFDGNVLEGQGLRHLEWPLHAEIMRARPDVN
                     VVGHSHAHYATVFGATDLELGPYNNHGVWFAEEGVPRFTQTSHIVTTVELGVAVAKQL
                     GKAEAILLEHHGIAFVGDNVIEATLAGIFLEWAARFQLDLVASKAAFSLPDAEESREK
                     RRRIYPPKAQRNYWMYFNRRLDRVEAVQGVGIASS"
     gene            3769..4698
                     /locus_tag="VFQ96_01780"
     CDS             3769..4698
                     /locus_tag="VFQ96_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0256551.1"
                     /translation="MSLPTTATVVGSGTMGPGIAATLKHAGVAVRVYDISREALARAE
                     KSFGMAEKVLDGLGAGVAGGTIAFLDDLAAALDGTEYVIEAVPEKHDLKASVIAQIEG
                     LVGDDVIIASNTSGIPISSLSPAMKHPGRLIGMHWSNPPHLIPMIEVIPGDQTDAAVV
                     ERVREVVKAFGYHPVLEKEIAGFIENRVLYAIMRECVALVQAGIATQEDVDTCVKWGI
                     GYKLSVVGPMRLLDMAGLDIYQSVSSYLNKELDASTDTPQVVKDLIAEGKLGFKTEGG
                     MYTYGPGDVDATRGAIVRGLITARKALETIDQV"
     gene            4703..5575
                     /locus_tag="VFQ96_01785"
     CDS             4703..5575
                     /locus_tag="VFQ96_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0256552.1"
                     /translation="MSLRWDADGFGHARGGGPDLAFRACGAGAPVVLLHGTSASHAVW
                     GPVADALADEAFVVSLDQRGHGRSFKPRPAEGGPDSGYDGASFADDIVTVLDALGLPT
                     AVLAGHSLGARNAWVAAARHPSRVRAVVAVDYVPYVESAVLDALQTRVAGGDRVFEGL
                     PEVEAYLQRRYPLMPADAVARRARWGYSRRAEGGWVPLAPGWALDRVVEGFRTSWEEE
                     FLRLAAPLVCLRGERSAVVSDDAWARARRARPDARWVDVAGADHYVPEERPLLVAEEI
                     RRVIRATADTASPG"
     gene            5700..6155
                     /locus_tag="VFQ96_01790"
     CDS             5700..6155
                     /locus_tag="VFQ96_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU0256553.1"
                     /translation="MPLFDGSLTVRCSTIAPLSRIPEAHSADGGNQTPQIAFTGVPDG
                     TVELALVCHDPDAPLPHGFTHWTVYGIPADAGRVDLAARGVREGPSGIGGRGWTGPQP
                     PAGHGPHHYYFWLYALGRAVAGEPTRQEFLADYADAVIEQSRLVGIFER"
     gene            complement(6298..>6804)
                     /locus_tag="VFQ96_01795"
     CDS             complement(6298..>6804)
                     /locus_tag="VFQ96_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0256554.1"
                     /translation="NVLVWGFGSIGRTLAPLLAALGAKVKGVARTGGPRDGFPVVTAR
                     ALPKELPKTDVLVGILPGAADTTGAIGADVFAALPERALVVNVGRGVTLDQKALADAL
                     RSRSIAGAALDVATPEPLPADDPLWEAPRLIVTPHAAGGRPLGANERIAHNLAALDGG
                     APFLHRAR"
BASE COUNT         1060 a         2320 c         2406 g         1018 t
ORIGIN      
        1 ggagagcgcg aggacccggg cgatggtgag ggcgcttcgc aacgcgtccg gcgacgagaa
       61 ctctgccgct ccccgcgccg tgtgaaagcc ccacccgcca gggcgcccga ccccgatgag
      121 ggcggcgggc acgaggtgcg agatgttgcc gaagtcggtt gcgaacggca gcgggggcgg
      181 ctctgtcacg aactcctcgc cgaagaagag cgcctccgcg tcgaggacga gcccggtgcc
      241 ctcctccacg tcgccgtcca gctcgatgtg ctcgagcagc aggcgcgacg ggccgacctc
      301 gtcggcggcg gccacgagtg ccgcgagcgc tcgaagagga gtctgggcgc gcccggaacg
      361 cagtgggcat cccgccgacg ccttcctcga ccgtgagccc cgcctcggcc agccggccgc
      421 gtagataggc cgaggttcga tgctcctcgt gcgccagttc cgggtgagcg aacacggcgt
      481 cgagcacctg ccgagtggcc tcttcgccct gggagatctc cttcagcacg gaatcgtcgc
      541 tcatggctct cttctcttca gcgcccggcg gctcggtggc aggccgcccc tccgttcggc
      601 ctggcgggct gactgatcag ccggccagat ggccgtggtc ggcagggtcg ccgtgcggtc
      661 ggtgcaggca cccgagcttt gtcggctttc gtacgcgact ttcgctggtg gtatcacgat
      721 gtacaggttg accgttacca tacgagaact tatacgcatg gtcaagggct tgctggcagg
      781 tctcgaggcc ggatatgtcg tgagtttccg gtctttttgc gtgcgccaga tccttgcgac
      841 gcccctcgtc ggcgaagaga gtcgccgtaa gaccctattg cgtgatgtga gaatcagttt
      901 gtaacatatg acaacaagct ggcccccgac ggaggaggcc cgcatgcaga gcaaaggggc
      961 acgaccggat gactacttcc cacagacggc atgccgaggt cgcaggggcg gggttcgccg
     1021 gcctcacgat ggcgaccgct cttgcacagc ggggctggtc ggtgcgggtg cacgagcgtg
     1081 gcaagaagct gcgcgaagag ggtgccggca ttctgctctg gcagaacagc ctcgcggtgc
     1141 tcgacgcgct cggcgtgacc gacacgttga tgacgcgctc aatgaccccg ccgttctatg
     1201 agacgcgcat gcagaaccga accgtctccg ccgaggccct ggagggcatc cgctggcgca
     1261 cgatgacccg gccgttcctg cacaagaccc tgctggatgc cgcccgggcc gccggggccg
     1321 aggtcatccc cggctctgag gttgcctccg cgaccgacga cggggtcatg acgctccgcg
     1381 acggcacgcg gctggaggcc gatctcgtcg tcggggccga cggcgtgggc tcgaacgtgc
     1441 gagattccct tggctttcgc tacgaccggc agcgttcccg cgacggcatc agccggttcc
     1501 tcgtcggccg gcgcaaggcg gatctcgcgg cgatcgagcc ggagaccgac tgggacaaca
     1561 tgatcgactt ctggaacctg gagccgcgtg tgctgcgagt gctctacacg ccggtgaacg
     1621 acaaggaact ctacctggct ctcatggcgc ccgccgcaga cgaggcgggc acccgggtgc
     1681 ccatcgacct cgacttgtgg acctccatcc accctcagct cgcgcccgtc ttggaagagg
     1741 tcgcgcgcaa ggagggcaag cactacggct accagacgac ccgcctcgac gaatggaccc
     1801 gcggccgggt cgccctggtc ggcgactcgg cgcacgctat gtgcccggcc ctcgcccagg
     1861 gcgccggatg ttcgatgatg aacgcgtaca ccctcgccca ggtggtctcc gacaatcccc
     1921 tcggcgacct gcccgacgcg atccgagagt gggagcgcct ggagcggccg tacaccgacc
     1981 gtgcccagac ccggtcgcag ggctacgccg acacgagaga catgtcgaag ggcaaccagt
     2041 tcaccggcga ggtgaacgag accacgctgt atgacccgac tgacccgcac cggcacgacg
     2101 cggtgcgggc gggctgagcg gcggccgtct caacggcggg ctcgccgccg agaggtcggg
     2161 cggccggagg gcgacggcga cccgcggctg ggaacgcgag aacgacacga gcaggaagac
     2221 gggaagggcg aagacgagga tgtctgcgaa cgagatcgaa gagtcgtacg aggtgcgcga
     2281 gtggttcggc ttcgtgcaag agatccggca cgaggggggc acgccgcccg atagcccgct
     2341 cgtgaaggcc gccgcgggag tcgtctttcg gaacccgttc gccgggcgtt ggcaagacga
     2401 cctcgccgcg ctgaccggcc ccagcgccgc actcggcacc gagcttgggc ggcgggcggt
     2461 ggcgcttctt gccggcctgc cggtcgaggg ctatggcaag ggcggcatcg ccggcctcga
     2521 cggtgagcag gaacacgtcg tcgcctgcgt cacgaccgtc ttcggcaacg cgttccgcgc
     2581 cgcggtgggc ggcggcagag cgtggatctc ctccgcatcg aagaccgccg ccgccggatc
     2641 gtcgatcgac atcccgctcg ccttcaagga tgagatctac gtgcgttcgc actacgatgc
     2701 cgtcactgtt caggtgcccg acgggccgcg cgccgacgag cttctgatcg tggtcgcggt
     2761 ctcatcgggc ggccggattc atcagcgggt cggcggcctc agccgagacg aggcgctcgc
     2821 cagattggcg tgagggccga cgagccgcag gagcgtctgg cacaagaaca tccggaacga
     2881 tccccaacga agaggaagtc atggagtcat gagttatacg tcagggcagt accagcagga
     2941 cgagtcgatc gcgaacatgc tcaccgacgt cgcccgagcc caccgcatcc tcgaactgga
     3001 gggccacggc gacatgtcga tgggtcatct gtcgctgcgc gacccgttcg gccgcggcct
     3061 ctggctgaag cgcgggaacc tcgcgctctc cgaggtggtc ggcgacgact tcatcctgat
     3121 cgacttcgac ggcaacgtgc tggagggtca gggcctgcgt cacctcgaat ggccgctgca
     3181 cgcggagatc atgcgggccc ggcccgacgt gaacgtcgtc ggccattccc acgcccacta
     3241 cgccaccgtg ttcggcgcca ccgatctgga acttgggccc tacaacaacc acggcgtctg
     3301 gttcgcggag gagggggtgc cgcgattcac gcagacgagc cacatcgtca ccacggtcga
     3361 gctcggcgtc gcggtcgcga agcaactcgg taaggcagag gcgatcctcc tcgagcacca
     3421 cggcatcgcg tttgtcggcg acaacgtgat cgaggccacc ctggccggca tctttctcga
     3481 atgggccgca cggtttcagc tcgacctggt cgctagcaaa gcggcgttca gcctgccaga
     3541 cgccgaagag agccgggaga agcgccgccg catctacccg ccgaaggcgc aacggaacta
     3601 ctggatgtac ttcaatcgca ggctcgaccg cgtcgaggct gtgcagggtg tcggcatcgc
     3661 ctcgtcctga ggcctcggcc cggaccggcc cggaagcgtg ggggtcggcg cccggcgccg
     3721 ctcggcccat cgactgacat ctatccgcat acagaaggag tacgcatcat gagtcttccc
     3781 accaccgcca ccgtcgtcgg gtcgggaacc atgggccccg gcatcgccgc gaccctcaaa
     3841 cacgcgggtg tcgccgttcg cgtctacgac atcagcaggg aggcgctcgc tcgagcggag
     3901 aagtcgttcg gcatggctga gaaagtcctc gacggcctcg gcgcgggtgt cgccggggga
     3961 acgatcgcct ttctcgacga cctggccgcg gccttggacg gcaccgaata cgtcatcgag
     4021 gcggtgccgg agaagcacga cctcaaagcg tcggtgatcg cccagatcga aggcctcgtc
     4081 ggcgacgacg tgatcatcgc cagcaacacc tccggcatcc cgatctcctc gctctcgccg
     4141 gcgatgaagc accccggccg cctcatcggc atgcactggt cgaacccgcc gcatctcatc
     4201 ccgatgatcg aggtgatccc gggagaccag acagacgccg ccgtcgtcga gcgggtgcgc
     4261 gaggtcgtga aggcgttcgg gtaccacccg gtgctcgaga aggagatcgc cggcttcatc
     4321 gagaaccgcg tgctttacgc gatcatgcgc gaatgcgtcg ccctcgtgca ggcgggcatc
     4381 gccactcagg aagacgtcga cacgtgcgtc aaatggggca tcggctacaa gctctccgtc
     4441 gtcggcccga tgcgcctgct cgacatggcg ggcctcgaca tctatcagag cgtctccagt
     4501 tacctcaaca aagagctgga cgccagcacc gacaccccac aggtcgtcaa agacttgatc
     4561 gccgaaggca agctcggctt caagaccgag ggcggcatgt acacctacgg gccgggcgac
     4621 gtcgatgcga cgcggggggc gatcgtgcgg ggcctcatca ccgcccgtaa ggcgctcgag
     4681 acgatcgacc aggtgtgacg aggtgagcct ccggtgggac gccgacgggt tcggccacgc
     4741 gcggggcgga gggcctgacc tggccttccg cgcctgtggc gccggggcgc cggtcgtcct
     4801 gctgcacggc acctcggcga gccacgccgt gtggggtccg gtcgccgacg cgctcgcaga
     4861 tgaggcgttc gtcgtctcgc ttgaccagcg cggccacggg cgaagcttca agccccggcc
     4921 tgctgaaggc gggccggatt ccggctacga cggggcgtct ttcgccgacg acatcgtcac
     4981 ggtgctagac gcgctggggc ttccgaccgc ggtgctcgca ggccattcgc tgggcgcgcg
     5041 caacgcctgg gtggcggccg cccggcatcc ctcgcgcgtg cgggccgtcg tcgcggtcga
     5101 ctacgtgccg tatgtcgagt ctgcggtgct cgacgccctg cagacgcggg tggccggtgg
     5161 agatcgggtg ttcgagggtc tgccagaggt cgaggcgtac ctgcagcggc ggtatccgct
     5221 gatgcccgcg gatgcggtcg cccggcgggc acgctggggg tatagccgca gggctgaggg
     5281 cggctgggtt cccctggcgc ccggttgggc cctcgaccgt gtggtcgaag ggttccgcac
     5341 ctcgtgggag gaagagttcc tgcgactcgc cgcgccgctc gtgtgtctgc gcggcgagcg
     5401 cagcgccgtc gtcagcgacg acgcctgggc acgggcccgc cgggcccggc ccgacgcgcg
     5461 gtgggtcgac gtggcgggtg ccgaccatta cgtgcccgag gagaggccgc tgctcgtcgc
     5521 cgaagagatc cggcgggtga tccgcgccac ggccgacacg gcgtcgcccg gctgacctgc
     5581 cctgccctgc cccggctctt cagaccatcc ggacccccac catttcgacc ctcgccgttc
     5641 gggctgcggg ccggccggct ggctcggcgg caacgcaacg agaagaagga gaagagatca
     5701 tgcccctttt cgatggctcc ctgacggtgc gctgctcgac catcgcaccg ctctctcgca
     5761 tccccgaggc gcattcggcc gacggcggca accagacgcc gcagatcgcg ttcaccggtg
     5821 ttcccgacgg cacggtcgaa ctcgcactcg tctgccacga ccccgacgcc ccgctgccgc
     5881 acggcttcac tcattggacg gtgtacggca taccggccga tgccggtcgg gtggacctcg
     5941 ccgcacgcgg cgtgcgcgag ggccccagcg ggatcggcgg gcgaggctgg acggggccgc
     6001 agccgccggc cggccacggc ccgcaccact actatttctg gctctacgcg ctcggccgcg
     6061 cggtcgcggg cgagccgacc cggcaggagt tcctggcgga ctacgcagac gccgtcatcg
     6121 agcagtcccg cctcgtgggg attttcgagc gttgacgctc ccccgccccg cccgtggcgg
     6181 gcccgcaggg tgtgggtgtg aggcgcggga ggcctgcgcg ggcctgcgag gtctagatgg
     6241 ctcggccccg gcgacggagg ttccttctgc gtcgacgacg ctgccgagag atgctctcta
     6301 gcgggcgcga tgcaggaatg gtgccccgcc gtcgagggcg gccagattgt gggcgatccg
     6361 ctcgttcgcg ccgagcggcc ggccgccggc ggcgtgcggc gtgacgatca gccggggcgc
     6421 ctcccacagc gggtcgtcgg ccggcagggg ctccggtgtg gcgacgtcga gcgcggcgcc
     6481 ggcgatcgaa cgggagcgga gggcgtcggc cagggccttc tggtcgagcg tgacgccgcg
     6541 ccctacgttc acgacgaggg cccgctcggg cagcgccgcg aagacgtcgg cgccgatcgc
     6601 cccggtcgtg tcggcggcgc cgggcaggat tccgaccagc acgtcggtct tgggcagctc
     6661 cttgggcaac gcccgggcgg tgaccaccgg gaagccgtcg cgcggtccgc cggtgcgggc
     6721 gacgcccttc accttggccc cgagcgcggc gaggagcggc gcgagggtgc gcccgatgct
     6781 tccgaagccc cagacgagca cgtt
//
LOCUS       DASTNL010000053         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1522780, whole genome shotgun sequence.
ACCESSION   DASTNL010000053 DASTNL010000000
VERSION     DASTNL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522780"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFQ96_01800"
     CDS             <1..388
                     /locus_tag="VFQ96_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEU0256555.1"
                     /translation="LADDEVGRIVDLVTRLGLDGIIATNTTTSRHGLRTSAEAVAAAG
                     PGGLSGPPVRARSLEVLRLIRARAPRLCVVSVGGVTSAADVAERLNAGASLVQGYTAF
                     LYAGPLWVRQINRRLSRGADGTWFAA"
     gene            complement(427..891)
                     /locus_tag="VFQ96_01805"
     CDS             complement(427..891)
                     /locus_tag="VFQ96_01805"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEU0256556.1"
                     /translation="MAETVTAAADGSSLGNPGPAGWAWYAGEDRWHAGGWPRSTNNRG
                     ELMAVIDLFDATAAARCPLVVICDSQYVINAVTTWMPAWKRRGWRKSDGKPVLNRDLV
                     ERLDAALAGRDYVFRWVKGHAGHPLNEAADERARGFAEAIRDGRQGLTGPGF"
     gene            complement(898..1503)
                     /locus_tag="VFQ96_01810"
     CDS             complement(898..1503)
                     /locus_tag="VFQ96_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HEU0256557.1"
                     /translation="MARRQTPGVRTEGVPDDGHQEASEEARQPAAGKGRATPSRRERE
                     AANKRPLVPADRRQAAKDARVRSRELRQQARSGMAAGEERYLTARDRGPQRRFVRDYV
                     DARRNVAELMIPAMIIIIVLSTIKITAVQLVSTFALWAYFTVAIIDSFLLSRRIVRRL
                     GERFGESHVQRGVRWYGIMRAMQLRVIRLPKPQVRRGQYPR"
     gene            1543..3033
                     /locus_tag="VFQ96_01815"
     CDS             1543..3033
                     /locus_tag="VFQ96_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0256558.1"
                     /translation="MTSEAGRRHETDDEWMRENVAGSMPASLADLASLIRIPSVSWPT
                     FDPQNVADSAAAVRRLALGTGLFETVEIRRAPVAPPAGSPAGGAGRDRAEGEPQGGGP
                     QEPELGQPAVVARRPARNGRPTILLYAHHDVQPEGAEGDWESAPFEPVVVGDRLYGRG
                     AADDKAGIMVHIAALRALAARFGDDLELGLVLYVEGEEEFGSRSFAAFLRENRELLAA
                     DAMVVADSDNWDARTPALTVALRGNVTFTLGVRTLAHASHSGMFGGAVPDAMLAMTRL
                     ITSLYDEDGSVAVAGLTTHEAPVPDYPVDDLRRDAAFLPGVAPIGTGPVRSRLWFQPA
                     ISITGIDAPSLATASNTLLPTVSVRVSCRVAPSQPAREAFEALARHLEDHAPFGAEVT
                     ISGVDLGEAFLADTDGWASLEMRRAMEFAWGRPPMLTGTGGSIPFIAELVREFPAAQI
                     LVTGVEDPDTRAHSPNESLHLGVFRRAATTEAVFLARAAARPAGRI"
     gene            3153..4043
                     /gene="coxB"
                     /locus_tag="VFQ96_01820"
     CDS             3153..4043
                     /gene="coxB"
                     /locus_tag="VFQ96_01820"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU0256559.1"
                     /translation="MRHKRRSRWAVLPLAALLTAVLAGCTQAQLHGFLPGFVKGEKPV
                     TTNTPLVDGLWVTAWIVLLIVGFTVWGLIIWTLVVYRRRKGQTGLPVQMQYNMPIEIF
                     YTVVPLILIFGFFSFTAHDQNIIEQRYQHPDQTIEVIAKQWSWDFNYKTENVYSPGIQ
                     AQPAPGGPQGSVVESKLPTLYLPVNKKVEILLTSRDVIHSFWVPAFLYKKDMFPDRIN
                     YMSVTPERIGEYAGKCAELCGEYHSQMLFNVKIVSDKVFMQHMNQLKSEGYSGQLSDS
                     YDRNQNLNGTGAPRENTPSK"
     gene            4117..>4647
                     /locus_tag="VFQ96_01825"
     CDS             4117..>4647
                     /locus_tag="VFQ96_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU0256560.1"
                     /translation="MSRGPNQTHRKGNLLVSWITSTDHKTIGYMYLMTSFVYFCLGGV
                     LALIIRAQLFEPGLHVVATKEQFNQLFTMHGTIMLLLFATPLFAGFANAIMPLQIGAP
                     DVAFPRLNALSFWLYFFGSLIVMMGFLTPQGAASFGWFAYVPLSTTTFSPGIGGNVWV
                     FGLALSGFGTILGAVNF"
BASE COUNT          728 a         1636 c         1513 g          770 t
ORIGIN      
        1 cctcgccgac gacgaggtcg ggcgcatcgt cgacctcgtg actcgcctgg gtctcgacgg
       61 catcatcgcg acgaacacga cgacgagccg tcacggcctc cggacctcgg cggaggcggt
      121 cgcagcggcc ggaccaggcg gcctctccgg cccgcccgtg cgggcccgtt ccctcgaggt
      181 gctccgtctc atccgggcca gggcgccgcg actgtgcgtc gtctcggtgg gcggggtgac
      241 gagcgccgcc gacgtggccg aacggctgaa cgccggagca agcctcgtgc agggttatac
      301 ggccttcctc tacgccgggc cgctgtgggt gcggcagatc aaccgccggc tctcccgcgg
      361 ggccgacggc acgtggttcg cggcgtgacg gccggctaag cgcgtatcgc ctggaacccg
      421 gactccctag aaccccggcc ccgtcaggcc ttgtctgccg tcgcggatcg cctcggcgaa
      481 gccgcgggcc cgctcgtccg ccgcctcgtt caacggatgg ccggcatgcc ccttgaccca
      541 ccggaagaca tagtcccgcc ccgcaagcgc ggcgtcgagg cgctccacga gatctcggtt
      601 aagcacgggc ttgccgtctg acttgcgcca gccgcgcctc ttccacgccg gcatccacgt
      661 cgtcaccgcg ttgatgacgt actgactgtc acagatgacg acgagcgggc accgtgccgc
      721 ggcggtcgcg tcgaacaagt cgatgaccgc catcagctcg ccccggttgt tggtgctgcg
      781 cggccagccg cccgcgtgcc agcggtcttc tcccgcatac caggcccagc cggccggccc
      841 ggggttgccc aacgacgagc cgtccgcggc cgccgtcacc gtctcagcca ccgcccgcta
      901 cctcgggtac tggccgcgtc ggacctgcgg cttcggcagg cggatgaccc gcagttgcat
      961 ggcccgcata atgccatacc agcgcacccc gcgctgcacg tggctctcgc cgaacctctc
     1021 gcccagccgc cgcacgatcc gccgtgagag aaggaacgag tcgatgatcg cgacggtgaa
     1081 gtacgcccag agcgcgaacg tgctcaccag ctgcaccgcg gtgatcttga tggtgctgag
     1141 cacgatgatg atgatcatcg ccgggatcat cagctcggcg acgttgcgcc tggcatccac
     1201 gtaatcgcgc acgaaacgac gttgtgggcc ccggtcgcgg gccgtcagat agcgttcctc
     1261 gccggccgcc atgccgctgc gggcctgctg gcgcagctcc cgagagcgca cccgggcgtc
     1321 tttcgccgcc tgacgccggt cggcgggcac gagcgggcgc ttgttcgccg cctcacgctc
     1381 gcgccggctc ggcgtcgcgc gacccttgcc cgccgcgggc tgccgcgcct cttctgaggc
     1441 ctcctggtgc ccgtcgtcgg gcacaccttc ggtacgcacc ccgggcgtct gtcgtctcgc
     1501 cacactcatc ctcggtcgaa tcgtcctctt aagattaccg gtatgacgag cgaggccggg
     1561 cggcggcacg agacagacga cgagtggatg cgcgagaacg tggcgggttc gatgccggcg
     1621 agcctcgcag atcttgcgtc gctcatccgt atcccttcgg tgtcgtggcc cacgttcgac
     1681 ccgcagaacg tcgcagacag cgccgccgcg gtgcgaaggc tcgccctggg cacggggctg
     1741 ttcgagaccg tcgagatccg cagggcaccg gtcgccccgc ccgcagggtc tccggccggc
     1801 ggggcgggga gagaccgagc cgagggggaa ccgcagggcg gcggcccgca agagccggaa
     1861 ctcggccagc ccgcggtcgt ggcccgccgg cccgcccgga acgggcgacc gactatcttg
     1921 ctctacgcgc atcacgacgt gcagcccgag ggtgccgaag gcgactggga gagcgcgccg
     1981 ttcgaacccg tcgtcgtcgg agaccgcctc tacggccgtg gcgccgccga cgacaaggcc
     2041 gggatcatgg tgcatatcgc cgccttgcgt gccctggccg ccaggttcgg cgatgacctc
     2101 gagctgggcc tcgtgctcta cgtcgagggg gaggaggagt tcggctctcg ctccttcgcc
     2161 gcgttcctcc gcgagaaccg cgagctgctc gcggccgacg cgatggttgt cgccgacagc
     2221 gacaactggg atgcccggac gccggccctg acggtcgcgc tgcgcggcaa cgtcacgttc
     2281 acgctggggg tgcgcaccct cgcgcacgcg tcgcattccg gcatgttcgg cggcgccgtg
     2341 cccgatgcga tgctcgccat gacgcgtctc atcacgagcc tgtacgacga agacggctcc
     2401 gtcgcggtcg ccggtctgac gacccacgag gcgcccgtgc ccgactaccc ggtggacgat
     2461 ctgcggcgcg atgccgcctt ccttccgggg gtggcgccga tcggcaccgg cccggtgcgc
     2521 tcccgtcttt ggttccagcc cgccatctcg atcaccggca tcgacgcgcc cagcctcgcg
     2581 acggcttcca acacgctgct accgactgtc tcggtacggg tcagctgccg ggtggcgccg
     2641 agccagccgg cccgcgaggc gttcgaggcc ctcgcccgcc acctcgagga tcatgccccg
     2701 ttcggggccg aggtgaccat cagcggtgtc gacttggggg aggcgttcct cgccgacacc
     2761 gacggctggg ccagtctcga gatgcgtcgc gcgatggagt tcgcatgggg cagacccccg
     2821 atgctgacgg gcaccggcgg ctccatcccg ttcatcgccg agctcgtgcg ggagttcccc
     2881 gcggcgcaga ttctcgtgac gggggtggaa gaccccgaca ctcgggccca cagccccaac
     2941 gaatctctcc acctcggggt tttccggcgg gcggcgacga ccgaggcggt ctttctcgcc
     3001 cgcgccgcag cccgcccggc gggccgtatt taaccggcca tccggcgtcg gcacgccgcc
     3061 acggggccgc aacccatggc cgtcacgcca catcgacgcg ataggctgaa agacgagcgc
     3121 gctacgcgcg aagttctcac gaaaggtcac cggtgcgcca caaacgtcgt tcccgctggg
     3181 ccgttctgcc gttggcggca ctgctcacag cagtgctcgc cgggtgcaca caggctcagc
     3241 tgcacggatt cctccccggc ttcgtaaagg gcgaaaagcc cgtcacgacg aacaccccgc
     3301 tggtcgacgg cctgtgggtc accgcgtgga tcgtgctgct catcgtggga ttcacggtgt
     3361 ggggcctcat catctggaca ctggtcgtct accggcgccg caaggggcag accggcctgc
     3421 cggtgcagat gcagtacaac atgccgatcg agatcttcta cacggtcgtg ccgctcatcc
     3481 tgatcttcgg cttcttctcg ttcacggcgc acgaccagaa catcatcgag cagcgctacc
     3541 agcatcccga ccagaccatc gaggtcatcg cgaagcaatg gtcgtgggac ttcaattaca
     3601 agacggagaa cgtctactcg ccgggtatcc aggcgcagcc ggcaccgggc gggccgcagg
     3661 gcagcgtggt cgagtcgaag ctgcctaccc tctacctacc ggtgaacaag aaggtcgaga
     3721 tcctgctcac ctcccgcgac gtcatccact ccttctgggt tcccgccttc ctctacaaga
     3781 aagacatgtt cccagaccgc atcaactaca tgtcggtcac gccggaacgc atcggggaat
     3841 acgccggcaa atgcgccgag ctgtgcggcg agtaccactc gcagatgctt ttcaacgtca
     3901 agatcgtctc agacaaagtc ttcatgcaac acatgaacca gttgaagagt gagggctaca
     3961 gcggccagct gagcgatagc tacgaccgca accagaatct caacggcacg ggggcgccgc
     4021 gtgagaacac accgagcaag taagtaaaaa gggcgaggcg taagagcaat gagtacgaca
     4081 acggcaccgg ctgcggccag ggctcagaca ccaccggtgt ctagggggcc caaccagacc
     4141 catcgcaagg gcaaccttct cgtttcgtgg atcacgtcga cggatcacaa gacgatcgga
     4201 tacatgtatc tgatgacgtc cttcgtctac ttctgtctgg gtggggtttt ggcgttgatc
     4261 atccgggcgc agcttttcga gccgggcctg catgtcgtcg ccacgaaaga gcagttcaac
     4321 cagctgttca cgatgcacgg cacgatcatg cttctgctgt tcgcgacgcc gcttttcgcc
     4381 ggcttcgcca acgcgatcat gccgctgcag atcggcgcgc ccgacgtcgc gttccccagg
     4441 ctgaacgccc tgtcgttctg gctttacttc ttcggcagcc tcatcgtgat gatgggcttc
     4501 ttgactccgc agggtgcggc gtcgttcggc tggttcgcgt atgtgccgtt atcgacgacg
     4561 acgttctcgc cgggtatcgg cggcaatgtg tgggtgttcg ggctggccct gtcgggcttc
     4621 ggcacgatcc tgggcgccgt gaacttc
//
LOCUS       DASTNL010000054         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_967640, whole genome shotgun sequence.
ACCESSION   DASTNL010000054 DASTNL010000000
VERSION     DASTNL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967640"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFQ96_01830"
     CDS             <1..766
                     /locus_tag="VFQ96_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0256561.1"
                     /translation="LSKHERNSMIMDLMAFRVPWRVRLGDAAGVARLEQSPTATLRWR
                     LLVAGGCGLAAVMAGGLAVSAHPSWWEFGGGVGDLLQKHGAHELAVGAAVVNTMLDTF
                     AVLALLLVAAIVVISGTNITDTILFVLVVACSWAVAWGIALLVDRPGGPVTNASEAFW
                     FRGLTYPSRPVTLSAALLAGILIITRRPGDRVIEICVGLTGLAAVVWARLYLGLDYPL
                     DAAAGIVTGVATTTVCYSGAAAFDGRRHARGRPTRL"
     gene            824..2005
                     /locus_tag="VFQ96_01835"
     CDS             824..2005
                     /locus_tag="VFQ96_01835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0256562.1"
                     /translation="MITPDDDGQQPNLTPRGEAADVSVIVPTRNEAGNIAPLTARLTK
                     ALHGLRAEVIFVDDSDDATPQVIGQVAAEAALPVRLIHRTAPEGGLSGAVVAGLAASD
                     APWCVVMDGDLQHPPELVPALVARGHADAADVVVASRHRPGGSTTGLDGRLRRTVSEA
                     STWLARGLFPHRLHGCTDPMSGFFAVRRDRLRLDAMHPRGFKILLEILARHELVVTEE
                     PFVFGVRSSGHSKASLGQGMRFAVQLAALRFGRLSEFGVIGAVGAVLNLVIMAGLIRA
                     GMWYLPAAIIGGAVTIAVNFALQEWLVFRDLRDGAHGFTGRLTRSAGFDTAETTLRTA
                     VLWLVVRFLFWPSVAVQALLLCVGFVARYAYRSLVVYRRSDLALEAATIDDRPPVEAA
                     R"
     gene            complement(2043..2390)
                     /locus_tag="VFQ96_01840"
     CDS             complement(2043..2390)
                     /locus_tag="VFQ96_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256563.1"
                     /translation="MPAFPTVVLMVFLGACAATGAWMSAVMVVWSAHRRIAAGHARGR
                     APRRAARRRGTSLYLILVLGFVLACVATGALSGDWFALKIALVLCAGIVLIGFASIGI
                     VRLAVKPGGSAQA"
     gene            2567..3400
                     /locus_tag="VFQ96_01845"
     CDS             2567..3400
                     /locus_tag="VFQ96_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256564.1"
                     /translation="MTTHRPANSSTDPRVAVRVPAPTVSFWAAKAVSTALGEAASDFS
                     IHVLPPVVAVLAGFVFFVAALAWQLHRRRYVPVSYWLAVAAVGVFGTMAADVLHVALG
                     IPYEITASLYAAVLAAVFVLWWLLERDLSVHDITTPRRELLYWAAVVATFAMGTAAGD
                     LTATGFGIGYLPSLIVFAALILLPLFGYRYLHFGGVFAFWFAYVLTRPLGASAADWLG
                     KSRSAGGLGLHSGWVALLFTVVLAALVAVEIGRRGRAAAASARHREAPAATPANADTA
                     A"
BASE COUNT          462 a         1254 c         1184 g          589 t
ORIGIN      
        1 cttatcgaaa catgagcgga actccatgat catggatctc atggctttcc gcgtaccctg
       61 gcgtgtgcgc ctcggcgacg ctgcgggagt cgcgcggctc gagcaatccc ccacggcgac
      121 cctgcggtgg cgactcttgg tcgccggcgg gtgcggcctc gctgcggtca tggccggcgg
      181 actggccgtc tcggcgcatc cgtcgtggtg ggagttcggc ggcggtgtgg gcgacctgct
      241 gcaaaaacac ggcgcccacg aactcgctgt cggcgccgcc gtcgtcaaca cgatgctcga
      301 caccttcgcc gtactcgctc tgctgctcgt cgcggcgatc gtcgtgatct cgggcacgaa
      361 catcaccgac acgatcctct tcgtcttggt cgtcgcctgc tcgtgggcgg tggcctgggg
      421 catcgcgctc ctcgtcgacc ggccgggcgg ccccgtgacg aacgcctcgg aggcgttctg
      481 gttccggggc ctgacctacc cgagcaggcc ggtgaccctg agcgctgcgc tcctggccgg
      541 catcctcatc atcacgcgtc ggccgggcga ccgggtgatc gagatctgcg tgggcctgac
      601 gggcctcgcg gcggtcgtct gggccaggct ctacctgggc ctcgactacc cactcgacgc
      661 ggccgccggc atcgtgacgg gagtcgcgac caccaccgtc tgctactcgg gcgctgccgc
      721 cttcgacggc cggcgccatg cccgaggccg gccgacgagg ctatgaccgg ccggccgtgg
      781 catcctttgc caatcctggg aacggcctcg atagcttcgc tgcatgatca cacctgacga
      841 cgacgggcag cagccgaacc tcactcctcg aggcgaggcg gcggacgtgt ctgtcatcgt
      901 ccccacccgc aacgaggccg ggaacatcgc cccgttgacg gcgcggctga cgaaggcctt
      961 gcacgggctg cgcgccgagg tgatcttcgt cgacgactcc gacgacgcca cgccgcaggt
     1021 catcggccag gtcgcggccg aggctgcgct gcccgttcgg ctgatccacc gcaccgcgcc
     1081 ggagggcggg ttgtcgggcg cggtcgtcgc ggggctcgcc gcgagtgacg ccccatggtg
     1141 cgtcgtcatg gacggcgatc tgcagcaccc gccagagctc gtgcctgccc ttgtggcgcg
     1201 aggccatgcc gacgcggcag acgtcgtcgt cgcctcccgc caccggccgg gcggcagtac
     1261 cacggggctc gacggcaggc tgaggcgcac cgtgtcggag gcatcgactt ggctggcccg
     1321 gggcctcttc ccacaccgcc tccacggctg caccgacccg atgtctgggt tcttcgccgt
     1381 gcgccgcgac cgcctacgac tcgacgcgat gcacccgcgc gggttcaaga ttctgctgga
     1441 gatcctcgcc cggcacgagc tcgtcgtcac cgaggagccc ttcgtcttcg gcgtgcgctc
     1501 ctccgggcat tcgaaggcct cgctcggcca gggcatgcga ttcgccgtgc agctggcggc
     1561 gctgcggttc ggcaggctct ccgagttcgg cgtcatcggg gccgtcggcg ccgtgctgaa
     1621 cctcgtcatc atggcgggtc tcatacgtgc cggcatgtgg tacctgccgg cggcgatcat
     1681 cggcggcgcg gtcacgatag ctgtgaactt cgcgttgcag gagtggctcg tctttcgcga
     1741 cctgcgtgac ggggcgcacg ggttcaccgg ccgcctcacc cgctccgccg ggttcgacac
     1801 ggccgagacg accttgcgca cggccgtact ctggctggtc gtacgctttc tcttctggcc
     1861 cagcgtcgcc gtgcaggcgc tactgctctg cgtcggcttc gtcgcccgct acgcgtaccg
     1921 ctcgctcgtc gtctaccgcc gttcggatct cgcgctcgag gccgcgacga tcgacgaccg
     1981 gccgccggtc gaggcggcgc ggtagcgagg ccctaccggc cgggcggcca gccccagact
     2041 cgctacgcct gtgccgaccc gccgggcttt actgccaacc ggacgatgcc tatgctcgcg
     2101 aacccgatca gcacgatccc ggcgcacaag accagcgcga tctttagggc gaaccagtcg
     2161 ccggagagcg ctcccgtcgc gacacatgcc agaacgaagc ccagcacgag gatcaggtag
     2221 agactcgtgc ccctgcgcct cgccgcgcgc cgcggtgccc tgccgcgcgc atggccggcc
     2281 gcgatgcgcc gatgcgccga ccagacgacc atgaccgcgc tcatccacgc gcccgtggca
     2341 gcgcaggcgc cgagaaagac catgaggacg acggtaggaa aggccggcac agagccttct
     2401 aagattcgcc ggcactccca ccgcgtctcg ggccggggcg gactgggaaa gcctccgcga
     2461 cccgatgccg atgcttccct cgcggcaaag cgcacgagag gcggccccgg ccgagcgacg
     2521 gccgattctc agaggcgtct ggggaaaggg ggagcacact tcacgcgtga cgacgcaccg
     2581 ccccgcaaac tcctcgaccg acccgcgcgt tgcggttcgc gtgcctgcac cgactgtctc
     2641 gttctgggcc gcgaaggccg tctcgacggc gctgggagag gcggcgtcag acttctcgat
     2701 ccacgtcttg ccgccggtgg tcgccgtgct cgcaggcttc gtgttcttcg tcgccgctct
     2761 ggcctggcag ttgcacaggc gccggtacgt gccggtctcg tattggcttg ccgtggcggc
     2821 ggtcggcgtc ttcggaacga tggccgccga cgtgttgcat gtcgcactcg gcatacccta
     2881 cgagatcacg gcgagcctct acgccgcagt gctggccgcc gtgttcgtcc tctggtggct
     2941 cctggagcgt gacttatcgg tgcacgacat cacgacgccg cgtcgcgaac tcttgtactg
     3001 ggcggccgtg gtggcgacgt tcgcgatggg caccgccgca ggcgacctga cggcgacggg
     3061 gttcggcatc ggctatctgc cctcgttgat cgtcttcgcc gcgctcatcc ttctgcccct
     3121 cttcggctac cggtatctgc atttcggggg cgtgttcgcc ttctggttcg cctacgtgct
     3181 cacccgtccg ctgggtgcgt cggcggcgga ttggctcggc aagtcccgta gcgcaggagg
     3241 cctcgggctg cacagcggct gggtcgcgct cttattcacg gtcgtgctcg ccgcgctggt
     3301 tgccgtcgag atcgggcgac gtggtcgggc ggccgccgcc tccgcccgac accgggaagc
     3361 ccccgcggca acgccggcaa acgctgacac cgccgcctga ggtgccctcg gcaccatcgt
     3421 gtcctcacgc gctgtggagc agcgtcgcaa gcaagacgag gagcagcaga gccatgtagc
     3481 cgagcgcgt
//
LOCUS       DASTNL010000055         8062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_228627, whole genome shotgun sequence.
ACCESSION   DASTNL010000055 DASTNL010000000
VERSION     DASTNL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228627"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFQ96_01850"
     CDS             <1..587
                     /locus_tag="VFQ96_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU0256565.1"
                     /translation="VSARDPESGLIVIKPSGIRFGDLTPENMVIVDVDGNVVEGTHKP
                     SSDTASHCYIYRQRPEVNGITHTHSRYATAFAAVARPIPCVLTAMADEFGGEIPVGPF
                     AIIGDDSIGRGIVATLSGHRSRAVLMQNHGVFTIGHGPRDAVKAAVMAEDVARTVHVA
                     RQGGTPIPLPPETVDRLYDRYQNVYGQNPEGSLA"
     gene            587..2104
                     /gene="araA"
                     /locus_tag="VFQ96_01855"
     CDS             587..2104
                     /gene="araA"
                     /locus_tag="VFQ96_01855"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEU0256566.1"
                     /translation="MPKLTTSLAAYEVWFLTGSQHLYGPETLAEVARQSREIADRLAD
                     ARNIPVRIVWKPVLTGTDAIRRVMLEANAADEVIGVIAWMHTFSPAKMWIQGLAALQK
                     PLLHFHTQANVALPWGEIDFDFMNLNQSAHGDREFGYIQTRLAVPRKTVVGHSSDPAT
                     TAKIGTWSRAAAGWAAVGRLRLARFGDNMRQVAVTEGDKTEAELLFGVQVNTWGVNEL
                     ASAVERASDAEIDALVDEYEERYDVAPELRRGGGRHRSLRDGAAIEIGLRSFLEEGDF
                     GAFTTTFEDLGSLPQLPGLAVQRLMAEGYGFGAEGDWKTAVLVRAAAVMGAGLPGGAS
                     LMEDYTYDMTPGREVVLGAHMLEVSPSLTTHRPRLEVHPLGIGGKDDPVRLVFTADPG
                     PGVVVALSDMRDRFRLVANAVRLVEPAAPLPKLPVGRAVWQPQPDFTTALTCWLQAGG
                     AHHTVMSTALGVEAFEDFARMTRTEIVVIDEGTRARDFERELEWSAAYHRLARGI"
     gene            2263..3561
                     /locus_tag="VFQ96_01860"
     CDS             2263..3561
                     /locus_tag="VFQ96_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0256567.1"
                     /translation="MAPELTVEQQEVFRLIESGRDHVFVTGRAGTGKSTLLNHLAWHS
                     EKQLVICAPTGVAALNVGGQTIHSLFRLPIGVIADHELDQPRALRSLLNTIDMLVVDE
                     VSMVDADLLDAVDRSLRQARQRAAEPFGGVQVVLFGDPYQLAPVPGRDPAARAYFADA
                     YRSRWFFDAHVWSETTLRIVELSQVHRQSEREFTDMLDAVRHGRVTKEIADRLNTVGA
                     RPAPTDGTITLATRNDAVRRINRRRLEGLSGPLLTAEAEIDGDFGSRAYPAETTLELK
                     EGAQVMFLRNDTAPGAEPPRWVNGTVGTVVKIDRNVVVEVEGQDHEVEPAVWERYRYS
                     YDAAQKKLTKDVVAEFRQFPLRLAWAVTIHKSQGKSYDAAVIDLGARAFSPGQTYVAL
                     SRLTRLDGLYLTRALRPSDVTVDRDVERFMTARRAASPAG"
     gene            complement(3524..4816)
                     /locus_tag="VFQ96_01865"
     CDS             complement(3524..4816)
                     /locus_tag="VFQ96_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256568.1"
                     /translation="MTADRGEAAPRQAVPRRWQAAIIVAFGLGGVTVTSWGPRMPAVQ
                     KELAASTATIGALLACVTVGSICGLLLSTPFLHRFGNRRTVGGAILTAALGMAGIGAG
                     TMAGSDPVVACAFAVTGLGIGLMDVLINVEASEIERRLGRIRMPLMHGAWSVGAAAGA
                     GIGAACSALRISAAHQFLGEAVLLALVSVPLVRAIPLRREEAGKREPRLARLARWARG
                     WLDWRLLLIGAVMLGVEFGEGSANSWLSLAAHGGHGFDATTAAALLIVFAISEAGARI
                     FGGPVVDRIGRPATIRLTAGIGIVGVALFILGGSPVLIIAGVALWAAGVSMGFPLGMS
                     AAAEGADAVSRVSVVASIGYFASLAGPPVVGVIAEQTGLITALWLVAVLFAAAFLASG
                     SLRPAAEAPRTRRPPAAGGMRGARVGATRRARQPGEPS"
     gene            complement(4830..6347)
                     /locus_tag="VFQ96_01870"
     CDS             complement(4830..6347)
                     /locus_tag="VFQ96_01870"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEU0256569.1"
                     /translation="MAGAADDEKRPTTRPSHTAPGVGATPTGSTAQAASTTQALATAQ
                     FPEGFLFGSATASYQVEGAVHEDGRGASIWDTFSHTPGATAGGATGDVACDHYHRLDS
                     DLDLMKSLNLNAYRFSIAWPRIQPTGHGAARAEGLDFYSRLVDGLLERGITPVATLYH
                     WDLPQSLEDAGGWTERATAEAFADYAALMGEALGDRVAVWTTLNEPWCSAFLGYASGV
                     HAPGRRDPVAALRAAHHLNLAHGLATKRLRQVVPGGKVSVTLNLHVVRPDTPADADAA
                     RQIDAVGNRIFTGPMFRGAYPQDLLDDTAGITDWSFVADGDVAQIHQDVDYLGVNYYS
                     TSRVRRFDGTGSANHSAGHNASPDSPWPGADRVEFLPQPGPHTEMGWNIDPGGLHEIL
                     MRMHDEFPNLALFVTENGAAYPDEVTIENGTKAVHDEARIEYLRRHILAARRALADGA
                     DLRGYFVWSLLDNFEWAYGYTKRFGIVRVDYDTQERTLKDSARWYARLAATKKVA"
     gene            6728..7783
                     /locus_tag="VFQ96_01875"
     CDS             6728..7783
                     /locus_tag="VFQ96_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0256570.1"
                     /translation="MRHVTISEIARQLGISTASASYALNGRPGVSETTRKRVLDLAAR
                     LDWRPSSSARALSRSRSDTIGMVLKRDPDLLGSEPYYMSLLRGVEDVLSEAGQGLLLR
                     MVGAARGADIAVYRRWAAERRVDGVIVLDLAVDDPRPELLHELGMPFVLHGIRLDPET
                     GEQVVEDLVTDARIIVDHMAELGHRRIVYITGPLFLAHEVRRRRAVAAEAARHGIDVI
                     FAECDYTSAGAERLMRERAAGWPQFTGVVSSNDVMALGVVTALRDLKADRFALVSWDD
                     SLLCNVCVPGITALARHPEVQGRRSARLLLQVLDGRELLPEPPIPSSLVIRDTSVAAA
                     FCGPLGVAAPVGADATE"
BASE COUNT         1184 a         2758 c         2864 g         1256 t
ORIGIN      
        1 acgtcagcgc gcgcgacccg gaaagtggtc tgattgtgat caagccaagc ggcattcgct
       61 tcggtgattt gacgccagaa aacatggtga tcgtcgatgt cgatggcaac gtggtcgaag
      121 gtacacacaa accctcatcg gacaccgctt cgcattgtta tatttaccgt cagcgtcctg
      181 aagtcaacgg catcacccac acccattcgc gttacgcaac ggccttcgcg gcagttgccc
      241 gcccgattcc gtgcgtcttg acggcgatgg cagacgagtt cggcggcgag atcccggtcg
      301 gcccgttcgc gatcatcggc gacgactcga tcggccgcgg catcgtcgcc accctctccg
      361 ggcaccgcag ccgcgccgtg ctcatgcaga accacggcgt gttcacgatc gggcacggcc
      421 cgcgcgacgc ggtgaaggcc gccgtgatgg cggaagacgt ggcacgcacc gtgcacgtgg
      481 cccgccaggg cggaaccccc atcccgctgc cgcccgagac cgtcgatcgc ctttacgacc
      541 gttaccagaa cgtgtacggc cagaacccag aaggaagcct tgcctgatgc cgaagctcac
      601 cacgtctctc gcggcctatg aggtctggtt tctgaccggc agccagcacc tgtacgggcc
      661 ggagaccctc gccgaggtcg cacggcagtc tcgcgagatc gcggatcggc tggccgacgc
      721 ccggaacatc cccgtgcgca tcgtgtggaa gccggtgctc accggcaccg acgcgattcg
      781 gcgggtcatg ctcgaggcga acgcagccga cgaggtgatc ggtgtgatcg cgtggatgca
      841 caccttcagc ccggcgaaga tgtggattca ggggctcgcc gcgctgcaga aaccgctttt
      901 gcattttcac acccaggcga acgttgcgct gccgtggggt gagatcgact tcgacttcat
      961 gaacctcaac cagtcggccc acggtgaccg ggaattcggc tacatccaga cacggctcgc
     1021 ggtgccgcgc aagaccgtgg tcggccactc cagtgacccc gccacgaccg cgaagatcgg
     1081 cacctggtcg cgcgccgccg ctggctgggc ggccgttggc cggctgcggc tcgcgcgctt
     1141 cggcgacaac atgcgccagg tcgcggtgac ggagggcgac aagacggagg cggagctgct
     1201 cttcggcgtg caggtcaaca cctggggcgt gaacgagctg gcctcagccg tcgaacgcgc
     1261 ctccgacgcc gagatcgacg cgcttgtcga cgagtacgag gagcgctacg acgtcgcgcc
     1321 ggagctgcgc cgcggcggcg ggcgacaccg gtcgctgcgc gacggcgccg cgatcgagat
     1381 cgggctgcgc tccttcctcg aagagggcga ctttggcgcg ttcaccacga cgttcgaaga
     1441 tctggggagc ctgccgcagc tgcccgggct tgccgtgcaa cgcctcatgg ccgagggcta
     1501 cggcttcggc gcggagggcg actggaagac cgcggtgctc gtgcgcgccg ccgctgtgat
     1561 gggcgcgggc ctaccgggtg gcgcctcgct catggaggac tacacgtacg acatgacccc
     1621 cggtcgtgag gtcgtgctgg gcgcgcacat gctcgaggtg agcccgtctc tcaccacgca
     1681 caggccgcgg ctggaggtgc atccgctggg aatcggcggc aaggacgacc cggtgcggct
     1741 cgtcttcacc gccgaccccg ggcccggcgt cgtcgtggcg ctctctgaca tgcgcgaccg
     1801 gttccggctc gtcgcgaacg ccgtgcgcct cgtcgagccc gcggcgcccc tgccgaagct
     1861 gcccgtcggg cgcgccgtgt ggcagcccca gcccgatttc acgacggcac tcacctgctg
     1921 gctgcaggcc ggcggcgcgc accacacggt catgtcgacg gcgctcggcg tcgaggcctt
     1981 cgaagacttc gcccgcatga cccgcaccga gatcgtcgtc atcgacgaag gcacgcgcgc
     2041 ccgtgacttc gagcgcgagc tggagtggag cgccgcctac caccgcctcg cccgcggcat
     2101 ctgacccggc ggccgcgacc gggcggtgcg ctgcaccggt gtggtgcgca ctgcggcgtc
     2161 cgccggtctc gcggggccgt gggtcggcct cccagcccgg cggtagagtc tcgtgaggag
     2221 tcgcgggttc cgtcgggcgg cgacgcgggc gggaggtgtg gcatggcgcc ggagctgacc
     2281 gtggagcagc aggaggtctt ccggctgatc gagtcgggcc gggatcatgt ctttgtcacc
     2341 ggccgcgccg gcaccggcaa gtcgaccctc ttgaaccatc tcgcctggca cagcgagaaa
     2401 cagctcgtca tttgcgcgcc gaccggtgtg gccgcgttga acgtgggtgg gcagacaatc
     2461 cattcgctct tccgcctgcc gatcggcgtg atcgccgacc acgagctcga ccagccgcgc
     2521 gccctgcgct ccttgttgaa cacgatcgac atgcttgtcg tcgacgaggt ctcgatggtc
     2581 gacgcagacc tgctcgacgc cgtcgaccgc tcgttacgcc aagcccgcca gcgcgccgcg
     2641 gagccgttcg gcggggtaca ggtggtgctc ttcggcgacc cctaccagtt ggcgcccgtg
     2701 ccgggccgtg acccggccgc acgcgcctac ttcgccgacg cgtatcgctc ccggtggttc
     2761 ttcgacgcgc acgtctggag tgagacgacg ctgcgcatcg tcgagctctc tcaggtgcac
     2821 cgtcaatcgg agcgggaatt caccgacatg ctcgacgcgg tgcggcacgg cagggtcacg
     2881 aaggagatcg ccgaccggct gaacacggtc ggcgcccggc ccgcgcccac cgacggcacg
     2941 atcaccctcg cgacccgtaa cgacgcggtg cgccgcatca accggcggcg tctggagggg
     3001 ctttccggcc cgctcttgac ggccgaggcg gagatcgacg gcgacttcgg ctcccgcgcc
     3061 tacccggccg agacgacgct tgagctgaag gaaggcgcgc aggtcatgtt cttgcgcaac
     3121 gacacggcgc ccggcgccga accgccacgt tgggtgaacg gcacagtcgg caccgttgtg
     3181 aagatcgatc ggaatgtcgt cgtcgaggtg gaggggcaag accacgaggt cgagcccgcc
     3241 gtctgggaac gctaccggta cagctacgac gccgcccaga agaagctgac gaaagacgtc
     3301 gtggccgagt ttcggcagtt tccgctgcgg ctggcgtggg cggtcacgat ccacaagtcg
     3361 cagggcaaga gttacgacgc ggccgtcatc gacctcggcg cccgcgcctt cagccccggt
     3421 cagacctacg tcgcgttgag ccggctgacg cggctcgacg gcctgtacct cacgcgggcg
     3481 ttgcggccct ctgacgtgac cgtggaccgc gacgtggagc gcttcatgac ggctcgccgg
     3541 gctgcctcgc ccgccgggta gcgccgaccc gggcgcctcg cataccgccg gccgcgggtg
     3601 gccgccgcgt tcgcggagcc tctgccgccg gccgcagcga cccggaggcg agaaatgcgg
     3661 cggcgaagag caccgcgacc agccagagcg ctgtgatgag tcccgtctgc tcggcgatga
     3721 cgccgacgac gggcgggccg gcgaggctcg cgaagtatcc gatcgaggcg acgacgctca
     3781 cccgcgagac ggcgtcggcg ccctccgcgg ccgccgacat gccgagcggg aagcccatcg
     3841 agactcccgc cgcccacagg gcgacgccgg cgatgatcag cacggggctg ccgccgagga
     3901 tgaagagcgc cacgccgacg atcccgatgc ctgccgtcaa ccggatcgtc gcgggccggc
     3961 cgatccggtc gacgacgggg ccgccgaaga tgcgggcgcc ggcctccgag atcgcgaaga
     4021 cgatgaggag cgcggcggcg gttgtcgcgt cgaagccgtg cccaccgtgc gcggccaggc
     4081 tcagccagct gttcgccgag ccttcgccga actcgacgcc cagcatgacg gcgccgatca
     4141 gaagcagccg ccagtcgagc cagccgcggg cccatcgggc gagccgtgcc aggcgcggct
     4201 cgcgtttgcc cgcctcctcg cgccgcaggg ggatcgcgcg caccagcggc acgctgacga
     4261 gtgcgaggag caccgcctcg ccgaggaact ggtgggcggc cgagatgcgc aaggcggagc
     4321 atgccgcacc gatgccggcg ccggccgcgg caccgaccga ccaggcgcca tgcatcagcg
     4381 gcatgcggat gcgacccagc cggcgttcga tttcggaggc ctcgacgttg atgagcacgt
     4441 ccatcagccc gatgccgagg ccggtcacgg cgaaggcgca ggccaccacg gggtcggagc
     4501 cggccatggt gccggcgccg atgcccgcca taccgagcgc ggcggtcagg atggcgccgc
     4561 cgaccgtgcg gcggttgccg aagcggtgca ggaacggggt ggagagcaga aggccgcaga
     4621 tcgagccgac cgtcacgcag gccagcagtg ccccgatagt ggcggtgctg gcggcgagtt
     4681 ccttctgcac ggccggcatg cgcggccccc acgaggtgac ggtgacgccg cccagcccga
     4741 acgcgacgat gatcgccgcc tgccagcgcc tgggcacggc ctgcctgggc gccgcctcgc
     4801 cccggtccgc ggtcaccgcg tcgggcgcct caggcgacct tcttcgtggc ggccagccgc
     4861 gcataccacc gtgcactgtc tttcagcgtc ctctcctgcg tgtcatagtc cacgcggacg
     4921 atgccgaacc gcttggtgta gccgtaggcc cactcaaaat tatcaaggag agaccagaca
     4981 aagtagccgc gcagatccgc accgtcggca agggcgcggc gtgccgccag gatgtgtcgg
     5041 cgcaggtact cgatgcgtgc ctcgtcatgc accgccttcg tgccgttctc gatcgtcacc
     5101 tcgtcggggt acgccgcgcc gttctcggtg acgaagagcg cgaggttggg gaactcgtcg
     5161 tgcatgcgca tcaagatttc atgcagcccg cccgggtcga tgttccagcc catctcggtg
     5221 tgcgggccgg gctgcgggag gaactccacc cggtcggctc ccggccaggg cgagtcgggg
     5281 gaggcgttgt gtccggccga atggttggcc gagccggtgc cgtcgaagcg gcgcacccgg
     5341 ctcgtcgagt agtagttgac gcccaggtag tcgacgtctt ggtggatctg tgccacgtcg
     5401 ccgtcggcga cgaacgacca gtcggtgatg cccgccgtgt cgtcgaggag gtcttgcggg
     5461 taggcgcccc ggaacatcgg gcccgtgaag atacggttgc cgaccgcgtc gatctggcgg
     5521 gcggcgtcgg cgtcggcggg tgtgtcgggg cgcacgacat gcaggttgag tgtgaccgac
     5581 accttgccgc ccggcacgac ctggcgcagc cgcttcgtcg cgaggccgtg ggcgaggttc
     5641 aggtggtgtg cggcacgcag cgctgcgacg gggtcgcgtc ggccgggcgc gtgaacgccc
     5701 gaggcgtaac cgaggaaagc cgagcaccac ggctcgttga gggtcgtcca gacggcgacc
     5761 cgatcgccca gcgcctcgcc catgagggcg gcgtagtcgg cgaaggcctc cgccgtcgcg
     5821 cgttcggtcc atccgcccgc gtcttcgagg ctctgcggca gatcccagtg gtagagggtg
     5881 gcgaccggcg tgatgccgcg ctccagcaga ccgtcgacaa gccttgagta gaagtcgagg
     5941 ccttcggccc gtgcggcacc gtgcccggtc ggctggatgc gtggccaggc gatggagaac
     6001 cggtaggcgt tgagattcag agacttcatg aggtcgaggt cggagtcgag ccggtggtag
     6061 tggtcacacg cgacgtctcc ggtcgccccg cccgcggtcg cgcccggggt gtggctgaac
     6121 gtgtcccaga tggaggcgcc gcggccgtct tcgtgcaccg caccctcgac ctggtacgac
     6181 gcggtcgccg agccgaacag gaacccttcg gggaactgcg cagtcgcgag ggcctgagtc
     6241 gttgacgcgg cctgtgccgt cgagccggtc ggtgtcgccc cgacgcccgg ggccgtgtgg
     6301 gagggtcgtg tcgtgggtcg tttctcgtcg tcggcggcac ccgccatggc tgcacccttg
     6361 cctttcctgc cagtgctctg gcttatcctg cctaccagtg aatcgtactg aagcgattaa
     6421 gtccattcgt cactgtgtaa ggtggttggc tattacgccc taagctaaac cggttaagta
     6481 ttatgcttcg gctgttcggc tgttcggctg ttcggctgtt cggttatccg gctgccggat
     6541 cgccgggatc tcaggtcgcc gggttccccg gccgtccggt ccaggctcgg cgactccggc
     6601 ggccggcgct aggccggagc gggtgagacg ggcggcgagc agccggcgcg atgcgaccgg
     6661 tcgggcgtgc ccgatattct cggaagcggc gacggtagag gctcgcaaag ctgtgaaggg
     6721 gggcgggatg cgccatgtga cgatcagcga gatcgctcgg cagttgggca tctccacggc
     6781 ttccgcgtcg tacgcgttga acggccgccc gggggtcagt gagacgacgc gcaagcgggt
     6841 actcgacctg gccgcacgac tcgactggcg gccgagttcc tccgcccgcg ccctgtcgcg
     6901 atctcgctcc gacacgatcg gcatggtgct gaagcgcgac cccgatctct tgggctccga
     6961 gccctactat atgagtctct tgcgcggggt tgaggacgtg ttgtctgagg ccggccaggg
     7021 ccttcttctg cgcatggtcg gtgcggcccg cggcgcggac atcgccgtgt accgccgttg
     7081 ggcggctgaa cggcgcgtcg acggtgtgat cgtgctcgac cttgccgtcg acgaccctag
     7141 gcccgagctt ctgcacgagc tcggcatgcc gttcgtcttg cacggcattc gcctcgaccc
     7201 cgagaccggc gagcaggtcg tcgaagactt ggtgaccgac gcgcggatca tcgtcgacca
     7261 tatggccgag cttggccatc ggcgcatcgt ctacatcacg ggcccgttgt tcctcgcgca
     7321 cgaggtaaga cggcggcgcg cggtggccgc ggaggcggcg cggcacggca tcgacgtgat
     7381 cttcgccgaa tgcgactata cgagcgccgg tgcggaacgg ctgatgcggg agcgtgcggc
     7441 aggctggccc cagttcaccg gcgtggtcag ctcgaacgac gtcatggccc tcggcgtcgt
     7501 gacggcgtta cgcgacctga aggccgaccg tttcgcgttg gtcagctggg acgactcgct
     7561 gctctgcaac gtgtgcgtgc cggggatcac ggcgctggcg aggcaccctg aggtgcaggg
     7621 ccggcgtagt gcccggctgc tgctgcaggt tctcgatggg cgagagctcc tgccggagcc
     7681 cccgatcccg agttccctcg tcatccgcga cacgagcgtc gcggccgcct tctgcggtcc
     7741 gctcggcgtc gcggcgcccg tcggcgccga cgcgacagag tgaccggccc cctcgcgccc
     7801 cgccccccgg cccctcgtgc cccggcccct cgtaccctgg ctcacccgct cgtcgtgccc
     7861 cggccccact cgaccgtcgt gatctaaccc gccacctgtg gcctttgcct ggttctggtt
     7921 accgaaagca ggccggaccg gcggggctgg gccggcttgg cggcgtgtcg cggtggaggg
     7981 cctgttccgg cctgctttcg gcaccgccgg gcgggtgact agagattgca gcgcggtgcc
     8041 gggccggtca gcggccgggt gc
//
LOCUS       DASTNL010000056        14017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_414447, whole genome shotgun sequence.
ACCESSION   DASTNL010000056 DASTNL010000000
VERSION     DASTNL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 14017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14017
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414447"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFQ96_01880"
     CDS             <1..646
                     /locus_tag="VFQ96_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0256571.1"
                     /translation="AVFHLAVRAPEEDVMSRAAGTPWWLGVKGADWRHPGGRLSSVEG
                     MGEHPVVHVSWNDAQAYCGWAGRSLPSEAQWECASRGGLAGAKYPWGDAEVDEGGWRA
                     NIWQGVFPTTNTREDGWLATAPVHTFEPNGYGLWQTVGNVWEWCQDYSDTHYYEHSPR
                     RDPAGPAQGAQRVLRGGSYLCHISYCNRYRCSARSQNTPDSSMGNAGFRTITTL"
     gene            complement(674..1606)
                     /locus_tag="VFQ96_01885"
     CDS             complement(674..1606)
                     /locus_tag="VFQ96_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0256572.1"
                     /translation="MNSRQLTVFLAVVDTGGVGKAARLLHVAQPSLSQTIRRLEAEAG
                     VALFTRVGRRLALTPAGEALITPARMTVNGLDQAARALSDIRRLHAGRLDIAALSTLA
                     TVPVAGLIGRFRRQHPDVVVRLAAPETAGQVAQLVRDGTAELGFAETPLDEEGLSVRP
                     LGTQRLVVVLPPGTAAKPAAHNHPDPCLPPILTLGELARLEWVAAPEGTSTRNLLEEA
                     LRTLGVTPRIAVETPHRDAIIPLVLAGAGATLLPETHAEDARAQGALIRATDPPVTRH
                     VALLHRTGEPSPAATAFLNQGFAPGTGAQAGLGG"
     gene            1678..3165
                     /locus_tag="VFQ96_01890"
     CDS             1678..3165
                     /locus_tag="VFQ96_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0256573.1"
                     /translation="MVSTARSGSPRPPWWRAALTEPARPAWVRARPGAHWLVVATVCV
                     GAFMGQLDASIVTVAFPTMQRDFAVPLGSVEWVALSYLLVLVSAVVGVGRLADMVGRK
                     LLYTYGFVVFALASLGCGLAPSLTWLIVFRLVQALGAAMLQANSVALIATSLPASILG
                     RGIGVQGAFQALGLALGPTVGGLLIGLGGWRWIFFVNVPAGVIGVVSAWFLLPRSRGL
                     AMRQPFDWSGLALFVPVAAALLLALSLGRDVGFARPGILALFLVSAAALGGFIVWERH
                     SVHPMVDLRLFGRMQFTAGIGSGFLSYAVLFGVLFVVPFLLERGLGMSTATAGLLLTV
                     LPAALGLTSPFAGRLADRVGARPLTVTGMILTAAGLGTMAVLPPTWPGLVVTLAVVGL
                     GQGLFTAPNNAAIMAAAPRTSSGSAGGLLNMTRGIGTSMGVALAGVTLGQTATHGGAD
                     TIGQGFTTTCVMLAALALVAAATTGLRGRPRRSPDGSGAAPETAG"
     gene            3186..3977
                     /locus_tag="VFQ96_01895"
     CDS             3186..3977
                     /locus_tag="VFQ96_01895"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0256574.1"
                     /translation="MKIVVLGGTGVLGRHVTREAAQRGHLVRVVSRHAPRSLPDGARH
                     IALDLAVRAGRQADAARAAALDAALTGADAVIDTVGGGRHVLVDGLRALLEEEARRGV
                     GHHVTISIVGCDVVPMPYYRTKVQQEGVVTAGPVPWSVLRATQFHDLVARLLAAAGKL
                     GLRPTGRILLQPVEPAALAPLLVDAAEQGPAGRLPDVGGPQVASLGELSALWARSGGG
                     RRLAVPLPVGGKVGAALKSGGLCLEAGGRSVGADFGTWLAAAGTP"
     gene            4116..4514
                     /locus_tag="VFQ96_01900"
     CDS             4116..4514
                     /locus_tag="VFQ96_01900"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEU0256575.1"
                     /translation="MARNPRRPRRALLAGAFDLVAVILFVVVGRAVHHEPGDFVDFLV
                     TLWPFVTGLVIGWLAAQAWRRPFRLPLPGIPIWVITVVGGMMFRVASSQGVAWAFVGV
                     AAGTLGVLFLGWRAIALVSVRGAVPAAGRS"
     gene            complement(4563..5390)
                     /locus_tag="VFQ96_01905"
     CDS             complement(4563..5390)
                     /locus_tag="VFQ96_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0256576.1"
                     /translation="MATLSPTLEFSDGHRIPQLGFGVYKIPDDEATRAVRVALETGYR
                     HIDTAAFYRNEAGVGRAVRESGIDRSEIFVTTKVWNDRHGYDETLRSFDESLSQLGLD
                     YVDLYLIHWPAPKQDRYVPTWHALERLRDEGRARSIGVSNFKPHHIERLLAESGTTPV
                     IDQVELHPMLPQRETREWCAAHSILVEAWSPLARGRMLSSPVIGRIAQKHGVTPAQCV
                     IRWHLEQGLVVIPKSAHAERIRSNFDVFDFTLDDSDLAAIAGLESGERTGRDPDDDD"
     gene            5709..8150
                     /locus_tag="VFQ96_01910"
     CDS             5709..8150
                     /locus_tag="VFQ96_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEU0256577.1"
                     /translation="MGITVVKRNGQREPYDANKINLAIEAASEGLGGDITWVTQIASE
                     LELTLFDGITTQQLDEAVIQVALQNVKDDPAFDTVAARLLLKTIYKRVLGDYADAAEL
                     AELHRQKFAEYVHRGVDEELLDPRFTNLYDLDRLAEVLDPTRDGMLKYIGVITLNNRY
                     GITARNGDPLEVPQFFWMRIAMGLALNEKDPTGIAMRFYEKMSRLDYLAAGSTLVNAG
                     TRYPQLSNCFVMEMQDDIEHIAKSVRDVMWITKGTGGIGLSVTKLRSQGSPIHSNNTV
                     STGPIPFMHTIDSVLRAVSRGGKKFGALAFYMENWHLDFGDFLDLKQNSGDPYRRTRT
                     ANTAAWLSDEFMKRVERDEPWYLFDPLEVADLNELYGKAFSERNNHYIAEAEAGRMRM
                     YKKIGARQQFKDMLMSLQTSAHPWLTWKDTINNRALNNNTGTIHLSNLCTEICLPQDR
                     ENIAVCNLASINLSRHLHDGVFDWQQLEESARLAVRQLDNLVDITLSSVPESEHSNKE
                     NRAIGLGVMGFTDVVERLGLCYESDEAYDVIDEVLEHVSYAAISESADLAEERGSYRN
                     FAGSRWSEGLVPFDTIDLMEKDRGVPVRVARTTRLDWDALRARVKNGMRNATLMAVAP
                     TASIGLVAGTTPGFDPQFTQIFSRSTSSGKFLEVNRNLVADLKRLGLWETVHDKILRS
                     QGDIQGIPEIPDELKAVYKTSFQLSPYAFLNVAARAQKWIDQAVSRNIYLESRDVDDM
                     IDIYTGAWERGVKTTYYLHVKPRHQSEQTSIKVNKAKEISRGRRGFGAFAAGSQEPKP
                     QRPVGAARRGFGGLA"
     gene            8147..8350
                     /locus_tag="VFQ96_01915"
     CDS             8147..8350
                     /locus_tag="VFQ96_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256578.1"
                     /translation="MSERTGTRSRAGAQESRARGSEERETQVREGAVQNAGARARRGG
                     GAHTDEYVVPVDPADEEQCEACQ"
     gene            8387..9379
                     /locus_tag="VFQ96_01920"
     CDS             8387..9379
                     /locus_tag="VFQ96_01920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEU0256579.1"
                     /translation="MGILGTGIQEGLLLKPVKYPWAMDLYDQAVANTWFPNEVQLGED
                     LADFKRMTDEERHAVTFLVSYFNPNELLVNKALAFGVYPYLKAAEAHVYLAKQMWEEA
                     NHSMAFEYVLDTFPIDRDAAYNSHLDVPSMLRKEEFEIKFIKRMTEVHLDITTTEGKR
                     DFVRNLVAYNIILEGIWFYSGFMVALSFRQRNLLRNFGSLIDWIVRDEALHLKFGMNL
                     ILTVLDENPDLQTPEFAAEIKNMVLEAVEMEAQYNRDLLPAGILGLNADYINQYVKYL
                     ADRRLEELGFETIYNVTNPAKWMAAANDTYELVNFFESINTSYESNAHASSTKH"
     gene            complement(9401..9631)
                     /locus_tag="VFQ96_01925"
     CDS             complement(9401..9631)
                     /locus_tag="VFQ96_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256580.1"
                     /translation="MAKRKWRELPPALRTAIIAAAAVQIALVGAAHADMSRRPAEQIR
                     GPKGLWRLATLVNFVGPVAYFRWGRLAPGTRP"
     gene            complement(9641..13792)
                     /locus_tag="VFQ96_01930"
     CDS             complement(9641..13792)
                     /locus_tag="VFQ96_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEU0256581.1"
                     /translation="MSTPPTLPQAAPPLVISYPPDLPVSARREEIAKVIRDNQVVIVA
                     GETGSGKTTQLPKICLEMGRRSIGHTQPRRLAARTIAERIADELGVEVGGLVGYQVRF
                     TDHAGPETRIKLMTDGILLNQMHHDRLLRAYDTIIIDEAHERSLTIDFLLGYLKRILP
                     RRPDLKVIVTSATIDPESFARHFASAGEPAPVVEVSGRTFPVEIRYRPRVDTADGKTT
                     GQRGTQPAARGDSGARGDADGAGSRGAAAGRRAPARPGDGDDDVNDAILAALAELDRE
                     KPGDVLVFLSGEAEIRDAADAIRAALSHAGDSRTEVLPLYGRLSAADQHRVFQRPAPG
                     VRRRIVLATNVAETSLTVPGIRYVIDAGMARISRYSARAKVQRLPIEPISQASAKQRS
                     GRSGRQSSGIAIRLYSEEDYAGRSEYTEPEILRTNLASVILQMLALGLGEIEDFPFLT
                     PPDRRGVKDGLGLLAELGAIDGAGRITPTGRSLARLPIEPRFGRMVLEAERLGVARPV
                     RAIVAGLTVQDPRERPMERRAAADQAHARFVNRDSDFLTLLNLWNYLHDKRKELSSSA
                     FRRLCKAEFLNYLRVREWQDVDRQLKRLTATLRRGHATNSTAPQGTEDHGIHQALLAG
                     LLSQIGLRDDRAASSPASRKRHGSPRGATPRRRTSDYIGSRQTRFTLHPSSALAKKPP
                     AAVMSAELVETSRLYARMNAAIDPQWAERLAGDLVKRAYGEPHWEKRQGQAVAYETVT
                     LYGVPIVTRRRVRYAPIDQALSRELFLRHALVAGEFDPHGGHTYDFLRANDRLRGRLA
                     EREERTRRRDVLYDDEAVFDFYDARIPPEVTGVRDFEAWWNRTRPENPGLLTMAADEW
                     GETVDPDTDAYPETWRQGDQTFALEYHFAPGSDNDGVSVLVPMPLLARLRPEAFTWQV
                     PGLRPDLVTALLKSLPKALRRHVVPAADWAAKVLADLPETPPEGRSLTEAAAASIRRL
                     TGTLAAAGDFSWERVPPHLRMSFCAVDGEGRVAGRDKDLAALQQRLSERARRSVARAV
                     APRPLSTGPAPARPGRGPAVAARDAAGRRGSGDGRGAPLSGDGRASATPNGIERSGIT
                     SWDFDEVPQTIDTRLPAPGGRYNVVRGYPSLALRGGAVDLVVLSTPEERRRVFPAAVL
                     RLVTGTVASPASYVADHLTPGEKLSLGVSGYRTTAALFDDCLTAIAADVLHARHPDGM
                     VFTRAEFEEVRDALHGVVVDRMFQTVALVARILQAARAAKKAVEETANLAWLTALTDM
                     RTQLEALVFPGFVTRTGLTALVQLPRYLAGVAHRLAVLPRDPARDRARMNELDEAAAL
                     FARAGGSLPLPADAPAALRHARWMLEEFRVSLFAQQLGTAEPVSLKRIRRALAEAKAT
                     ARPGA"
     gene            13852..>14017
                     /locus_tag="VFQ96_01935"
     CDS             13852..>14017
                     /locus_tag="VFQ96_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEU0256582.1"
                     /translation="MTNRLAEELSPYLRAHADNPVDWYPWGAEAFAEARRRDVPILLS
                     IGYAACHWCHV"
BASE COUNT         2065 a         4625 c         5067 g         2260 t
ORIGIN      
        1 cgcggtcttc catctggccg ttcgggcgcc ggaggaggac gtcatgagcc gggcggcggg
       61 tacaccgtgg tggcttggtg tgaagggcgc cgattggcgg catccgggtg gccggctttc
      121 ctcggtcgag ggcatggggg agcatccggt ggtgcatgtg tcgtggaatg acgcgcaggc
      181 gtattgcggc tgggcgggca gaagcctgcc gtcggaggcg cagtgggagt gtgcttcgcg
      241 gggcggtctt gccggggcga agtacccgtg gggggacgcg gaggtcgatg agggcggctg
      301 gcgggccaac atttggcagg gcgtcttccc gaccacgaat actcgggagg acggctggtt
      361 ggcgaccgcg ccggtccaca ccttcgagcc gaacgggtat ggcctgtggc agacggtggg
      421 gaatgtgtgg gagtggtgtc aggactactc ggacacgcac tactacgagc actcaccccg
      481 ccgggacccg gccggcccgg cgcagggtgc gcagcgggtg ttgcggggcg gctcgtatct
      541 gtgccacatc tcgtactgca atcggtaccg ttgctcggcc cgttcccaga acacgcccga
      601 ctcctcgatg ggcaacgccg gattccgcac catcaccacc ctttgagggt gagccggacg
      661 cggctctgac ccgtcaccct ccgaggccgg cttgcgcgcc ggttcccggc gcgaagccct
      721 ggttgaggaa ggctgttgcg gcgggggagg gctcgccggt gcggtggagg agcgcgacgt
      781 gtcgggtgac gggtgggtcg gtggcgcgga tcagtgcgcc ttgtgcgcgg gcgtcttcgg
      841 cgtgcgtctc ggggagcagg gtggcgccgg caccggcgag gacgaggggg atgatggcgt
      901 cgcggtgtgg ggtctcgacg gcgatgcgcg gggtcacgcc gagcgtgcgt aaggcctctt
      961 cgaggaggtt tctggtggag gttccctccg gggcggcgac ccattccagc cgggccagtt
     1021 cgcccagggt caggatcggg gggaggcatg ggtcggggtg gttgtgcgcc gcgggctttg
     1081 cggccgtgcc ggggggcagg acgaccacga ggcgttgcgt gccgagcggg cgcacgctga
     1141 ggccttcctc gtcgagcggg gtctcggcga agccgagctc cgcggtgccg tcgcgcacga
     1201 gttgggcgac ctggcctgcc gtctccggtg cggccagccg taccacgacg tcggggtgct
     1261 ggcgtcggaa gcggccgatg aggcccgcga cggggaccgt ggcgagggtc gagagggctg
     1321 cgatatcgag ccggccggcg tgcaggcgcc ggatatcgga gagtgcccgt gctgcttggt
     1381 ccagcccgtt cacggtcatg cgggcgggtg tgatgagcgc ctcgcctgcc ggcgtgaggg
     1441 cgaggcggcg gccgacgcgc gtgaacaggg cgaccccggc ctcggcctcc aaccggcgga
     1501 tcgtctgcga caaagagggc tgggcgacgt gcagaagccg ggcggccttg ccgacaccgc
     1561 cggtgtcgac gacggcgaga aacacggtca actgacgcga gttcatagct tcacattatc
     1621 gaaaacaaca aataatgatc tttgacgtat gcgttgggcg tgaggtggac tagatccatg
     1681 gtgtccacgg cgcgttccgg ctctccccgt ccgccgtggt ggcgcgccgc cctgacggag
     1741 ccggcgaggc cggcatgggt gcgggcccgg ccgggcgcgc actggttggt cgtggcgacc
     1801 gtatgcgtgg gcgcattcat gggccagctg gacgcgagta tcgtcacggt cgccttcccc
     1861 acgatgcagc gggacttcgc cgtgccgttg ggatcggtgg aatgggtcgc cctctcttac
     1921 ctgctggtgc tcgtctcggc cgtggtcggc gtgggccggc tggccgacat ggtcggccgc
     1981 aagctgctct acacctacgg gttcgtcgtc ttcgccctgg cctcgctggg ctgcgggttg
     2041 gccccgtcgt tgacctggct gatcgtgttc cgcctcgtcc aggcgttggg cgccgcgatg
     2101 ctgcaggcga acagtgtcgc gttgatcgcg acctcgctgc cggcctcgat cctcgggcgg
     2161 ggtatcggcg tgcagggtgc gttccaggcg ttgggtctcg ccctggggcc cacggtcggc
     2221 ggcctcttga tcgggttggg cggctggcgg tggatcttct tcgtcaacgt gccggccggc
     2281 gtgatcggcg tggtctcggc ctggttcctc ctgccgcgga gccgtggcct tgccatgcgg
     2341 cagcccttcg actggagcgg cctcgccctt ttcgtgccgg tggccgcggc gttgcttctc
     2401 gcgctctccc tgggccgcga cgtgggtttc gcgcggcccg gcatcctcgc cctgttcctc
     2461 gtatcggctg ccgcgctcgg cgggttcatc gtatgggagc ggcactcggt ccaccccatg
     2521 gtcgacctgc gcctgttcgg tcggatgcag ttcaccgccg gtatcggcag cggattcctc
     2581 tcgtacgccg tgctgttcgg ggtgctcttc gtcgtgccgt tcctcctgga gcgggggttg
     2641 ggcatgtcga ccgcgacggc cgggctgctt ctgaccgtgc tgcccgccgc gctgggcctg
     2701 acctccccct tcgccggccg gctggcagac cgggtgggcg cgcgccctct gaccgtgacc
     2761 ggcatgatcc tgaccgccgc cggccttggt accatggcgg tcctcccgcc cacctggccc
     2821 gggctcgtcg tcaccctcgc cgtcgtgggg ctggggcaag gcctcttcac ggcgccgaac
     2881 aacgcggcga tcatggccgc cgccccccgc acctcttccg gctccgcagg cggcctcttg
     2941 aacatgacgc gaggcatcgg cacctcgatg ggcgtcgccc tggccggagt cacgctgggc
     3001 cagaccgcga cacacggcgg cgccgacacg atcgggcagg gcttcaccac cacctgcgtc
     3061 atgctcgccg ccctcgctct cgtcgcggcc gccacaacag gactccgcgg cagaccacga
     3121 agatcccccg atgggtccgg cgcggcaccg gagacggccg gatagtctcg agacgggagg
     3181 tcgccatgaa gatcgtcgtt ctcggcggca ccggggtgct cggccggcac gtgactcgtg
     3241 aggcggccca gcgcggccac cttgttcggg tcgtcagcag gcatgccccg cgctcgttgc
     3301 ccgacggggc ccgccacatc gccctcgacc tcgcggtgcg agccggcaga caggctgacg
     3361 ccgcccgggc ggcggcgctc gacgccgcgc tgaccggggc ggacgccgtg atcgacacgg
     3421 tgggcggcgg ccggcacgtg ctggtcgacg ggctgcgcgc gctccttgaa gaggaggcca
     3481 ggcgcggcgt cggccaccac gtcacgatct ccatcgtcgg gtgcgacgtc gtgccgatgc
     3541 cttattaccg cacgaaggtg caacaagagg gggtcgttac ggcggggccg gtgccgtgga
     3601 gcgtgctgcg ggcgacccag ttccacgacc tcgtcgcgag gctcctcgcc gcggccggca
     3661 agctcggcct gcggcccacc gggcgtatcc tgctgcaacc cgttgagccc gccgcgctcg
     3721 cgccgctcct cgtcgacgcg gcggagcagg ggcctgcggg ccggctgccc gacgtgggcg
     3781 gcccgcaggt ggcgagcctc ggtgagctct ctgctctttg ggctcgatcg ggcggcgggc
     3841 ggcgcctcgc cgtgccgctg cccgtcggcg gcaaggtggg cgccgcgctg aagagcggcg
     3901 ggctgtgcct ggaggcaggc ggccgcagcg tgggagcgga tttcggcacc tggttggcgg
     3961 ccgccggcac cccgtgaacg gcgtgccgtc acggctccgg tcgggatcgg gtcggcctcg
     4021 acgggcggct gagcgtcggg cccttctcgt ccggggacgg ccgccgagcc agggcccagc
     4081 tctggggcgc cctcctcgcc tcttaagatt gggtcatggc acgaaacccg cgacgcccgc
     4141 gccgcgccct tctggccggc gccttcgacc tggtcgccgt catccttttc gtcgtcgtcg
     4201 gccgggcggt gcaccacgag ccgggagact tcgtcgactt cctcgtcacc ctgtggccgt
     4261 tcgtgacggg gctcgtcatc ggctggctgg cggcgcaggc ctggcggcgc cccttccggc
     4321 tgccattgcc ggggatccca atctgggtca tcaccgtggt gggcggcatg atgttccgcg
     4381 tcgcctccag ccagggcgtg gcctgggcgt tcgtcggcgt ggccgcgggc acgctgggcg
     4441 tgctcttcct cggctggcgg gcgatcgccc tcgtcagcgt gcgtggtgcc gtgcctgcgg
     4501 cggggcgctc ctaggccttg gcgggggccc ggcgctctga gggcctgtcc ggccccagaa
     4561 gctcagtcgt cgtcgtccgg gtcgcggccc gtgcgctcac ctgactcgag ccccgcgatc
     4621 gcggcgaggt cgctgtcgtc gagagtgaag tcgaagacgt cgaagttgct gcggattcgc
     4681 tcggcgtgcg cggacttcgg gatgacgacg agcccctgct caagatgcca ccggatgacg
     4741 cactgcgccg gcgtcacgcc gtgcttctgc gcgatgcgcc cgatgacggg ggaggagagc
     4801 atgcgcccgc gggcgagcgg cgaccaagcc tcgacgagga tgctgtgtgc cgcgcaccac
     4861 tcgcgggtct ctcgctgcgg gagcatcggg tgcagttcta cctggtcgat gacgggggtc
     4921 gtccccgact ccgccaagag gcgttcgatg tggtgcggct tgaagttcga gacgccgatc
     4981 gagcgcgccc gaccctcgtc gcgcagcctc tccagcgcgt gccaggtggg cacgtagcga
     5041 tcctgcttgg gcgccggcca gtggatgaga tacaggtcga cgtagtcgag ccccagctgg
     5101 ctgaggctct cgtcgaacga gcgcagggtc tcgtcgtagc cgtgccggtc gttccagacc
     5161 ttggtggtga cgaagatctc ggagcggtcg atgccgctct cgcgcaccgc tcgcccgacg
     5221 ccggcctcgt tgcggtagaa ggcggccgtg tcgatgtgcc ggtacccggt ctccagcgcg
     5281 acacgcacgg cccgggtcgc ctcgtcgtcg ggaatcttgt atacgccgaa cccgagttgc
     5341 gggatacggt ggccgtcgga gaactccaga gtcggtgaca aggtggccat gaaccccatt
     5401 atgccgggtg tcgcaggtgg ggaatatgct gtgggagcgg caccgggtgg ggcgacgggg
     5461 taaatacaac acgccactac atctggggct gacgggggaa ggcaagcccg ttatatagtg
     5521 gttcgacggt cagagagggc cctgcgcgag gcgcatgatc gtcgcggggg acagcttctc
     5581 gccgccctgc ccgcgtgctg cggcgagacg gcggggaagc attgaggaaa ggccctggca
     5641 ggcgcgacgc cgatcgtcgg tgacgtggcg gatgcgctcg cggcggccgg ggcagaggga
     5701 gcggagacat ggggatcacc gtcgtcaagc gcaacgggca acgcgagccg tacgacgcga
     5761 acaagatcaa cttggcgatc gaggcggcct ccgaggggct cggcggtgac atcacctggg
     5821 tcacgcagat cgccagcgag ctggaactca ccctgttcga cggcatcacg actcagcaac
     5881 tcgacgaggc cgtcatccag gtcgcgctgc agaacgtgaa agacgacccg gccttcgaca
     5941 cggtggcggc gcggctgctt ctgaagacga tctacaagcg ggtgctcggc gactacgccg
     6001 acgcggccga gctggccgag ctgcaccggc agaagttcgc cgaatacgtg catcgcggtg
     6061 tcgacgaaga gctgctcgac cctcgtttca cgaacctcta cgacctcgac cgcctcgccg
     6121 aggtcttaga cccgacacgc gacggcatgc tgaaatacat cggggtcata accctcaaca
     6181 accgctacgg catcacggcg cgtaacggtg acccgctcga ggtgccgcag ttcttctgga
     6241 tgcgcatcgc gatggggctc gccctgaacg agaaagaccc taccggcatc gcgatgcgct
     6301 tctacgagaa gatgtcgcgc ctcgactatc tcgcggccgg ctccaccctc gtcaacgccg
     6361 gcacccggta tccgcagctg tcgaactgct tcgtcatgga gatgcaagac gacatcgagc
     6421 acatcgccaa gagcgtgcgc gacgtcatgt ggatcacgaa gggcaccggc ggcatcgggc
     6481 tgtcggtgac gaagctacgc tcgcagggct cgcccatcca ctccaacaac acggtctcca
     6541 ccggcccaat cccgttcatg cacacgatcg actcggtgct gcgggccgtc agccggggcg
     6601 gcaagaagtt cggtgcgctg gccttctaca tggagaactg gcatctcgac ttcggcgact
     6661 tcctcgacct gaagcagaat tccggcgacc cctaccggcg cacccgcacg gcgaacacgg
     6721 cggcgtggct gagtgacgag ttcatgaagc gtgttgagcg cgacgagccg tggtacctct
     6781 tcgacccgct cgaggtcgcc gacctcaacg agctgtacgg caaggcgttc agcgagcgta
     6841 acaaccacta catcgccgag gccgaggccg gccggatgcg catgtataag aagatcggcg
     6901 cccgtcagca gttcaaagac atgctgatga gcctgcagac gagcgcccac ccgtggctga
     6961 cgtggaagga cacgatcaac aaccgcgccc tcaacaacaa cacgggcacg atccacctct
     7021 ccaacctgtg caccgaaatc tgcctgccgc aagaccgcga gaacatcgcc gtgtgtaacc
     7081 tcgcctcgat caacctctcc cggcacctgc acgacggcgt cttcgactgg cagcagctgg
     7141 aagagagcgc ccggctggcc gtgcgtcagc tcgacaacct ggtcgacata accctgtcga
     7201 gcgtgccgga gagtgagcac tccaacaaag agaaccgggc gatcggcctc ggcgtcatgg
     7261 gcttcaccga cgtggtcgag cggctcggcc tgtgctacga gtccgacgag gcctatgacg
     7321 tgatcgacga ggtcttggag cacgtctcct acgccgccat ctcggagagc gcagaccttg
     7381 ccgaggaacg cggctcgtat cgcaacttcg ccggctcccg ctggtcggag ggcctcgtgc
     7441 ccttcgacac gatcgacctg atggagaaag atcgcggcgt cccggttcgg gtcgcacgca
     7501 cgacgcgcct cgactgggac gcgttgcgcg cgcgggtgaa gaacggcatg cgcaacgcga
     7561 cgctcatggc cgtggcgccg accgcctcca tcggtctcgt cgcgggcacg acacccggct
     7621 tcgacccgca gttcacgcag atcttcagcc gctccacctc gtctggcaag ttcctcgagg
     7681 tgaaccgcaa tctcgtcgcc gacctgaagc gcctgggctt gtgggagacg gtgcacgaca
     7741 agatcttgcg cagccagggc gacattcaag gcattcccga gattcccgac gagttgaagg
     7801 cggtctacaa gacgagcttc cagctctcgc cgtacgcgtt cttgaacgtg gcggcccggg
     7861 cacagaagtg gatcgaccag gccgtcagcc gcaacatcta cttggagagc cgcgacgtcg
     7921 acgacatgat cgacatctac acgggcgcgt gggagcgcgg cgtgaagacc acgtactacc
     7981 tgcacgtgaa gccccgccac cagtcggagc agacatcgat caaggtgaac aaggcgaaag
     8041 agatctcccg cggccgtcgg ggcttcggtg ccttcgcggc cggctcgcag gagcccaagc
     8101 cgcagcggcc ggtcggggcg gcgaggcgag gcttcggggg gctggcatga gcgagcggac
     8161 gggcacgcgt tcgcgggccg gggcgcagga gtcccgagca cgggggtccg aggagcgcga
     8221 gacccaggtg cgagagggtg ctgtgcaaaa cgcgggcgcc cgggcgcgcc ggggtggtgg
     8281 cgcccacacg gacgaatacg tggtgccggt cgacccggcc gacgaggagc agtgcgaagc
     8341 ctgccagtag gcaccctgcc ggcaggcaca aggaggagaa gagacgatgg gaattctggg
     8401 taccggcatt caagaggggc tgctgttgaa gccggtcaag tacccgtggg cgatggacct
     8461 gtacgaccag gcggtcgcga acacctggtt ccccaacgag gtgcagctgg gcgaggatct
     8521 tgccgacttc aagaggatga ccgacgagga acgccacgcc gtgacgtttc tcgtcagcta
     8581 cttcaacccg aacgagctgc tcgtcaacaa ggctctcgcg ttcggcgtct acccgtacct
     8641 caaggcggcg gaggcgcacg tctacctcgc caagcagatg tgggaggagg cgaaccattc
     8701 gatggcgttc gagtacgtgc tcgacacgtt ccccatcgac cgcgacgccg cctacaactc
     8761 ccacctcgac gtgccgtcga tgctgcgcaa agaagagttc gagatcaagt tcatcaagcg
     8821 gatgaccgag gtgcatctcg acatcacgac gaccgagggc aaacgcgact ttgtgcgcaa
     8881 cctcgtcgct tacaacatca ttctcgaagg catctggttc tactcgggct tcatggtggc
     8941 cctttcgttc cgccagcgga accttctgcg caacttcggc tcgctcatcg actggatcgt
     9001 gcgtgacgag gcgctgcatt tgaagttcgg catgaacctc atcctcaccg tgctcgacga
     9061 gaaccccgac ttgcagacgc cggagttcgc cgccgagatc aagaacatgg tgcttgaggc
     9121 ggtcgagatg gaggcgcagt acaaccgcga tctgctgccc gccggcatcc tgggcctgaa
     9181 cgccgactac atcaaccagt acgtgaaata cctcgccgac cggcggctgg aggaactcgg
     9241 attcgagacg atctacaacg tcacgaaccc cgcgaagtgg atggcggcgg cgaacgacac
     9301 ctacgagctc gtcaacttct tcgagtcgat caacacgtcg tacgagtcga acgcgcacgc
     9361 gagttcgacc aagcactgag cgggtgggcc gccgcgcagc ctatggccgg gtgccaggcg
     9421 cgaggcggcc ccaccggaag taggcgaccg ggccgacgaa gttcaccagc gtggccagtc
     9481 gccacaggcc cttcgggccg cggatttgtt cagccgggcg gcggctcatg tcggcgtggg
     9541 ccgcccccac cagggcgatc tgcacggccg ccgcggcgat gatcgcggtg cgcaaggccg
     9601 gagggagttc tcgccatttt cgcttcgcca taggcggagc ctacgcgccg ggccttgccg
     9661 tggcctttgc ctccgcgagg gcgcgccgga tgcgcttcag cgacaccggt tcggcggtgc
     9721 ccagctgctg ggcgaaaagg ctcacccgga attcctccag catccaccgc gcgtgccgga
     9781 gggcggccgg ggcgtcggcc ggcagcggca ggctgccgcc ggcccgggcg aagagcgcgg
     9841 cggcctcgtc gagctcgttc atgcgggccc ggtcgcgcgc cgggtcgcgc ggcagcacgg
     9901 ccaaccggtg cgcgacgccg gccaggtacc ggggcagctg taccagcgca gtcaggccgg
     9961 tacgggtgac gaagccgggg aagacgagcg cctccagctg ggtgcgcatg tcggtgagtg
    10021 ccgtaagcca ggcgaggttc gcggtttcct ccacggcctt tttggccgcc cgggcggcct
    10081 ggaggatgcg ggcgacgagc gcgaccgtct gaaacatacg gtcgacgacc accccatgca
    10141 gggcgtctcg tacctcttcg aactcggcac gggtgaagac catgccgtcc ggatgccggg
    10201 cgtggagcac gtcggcggcg atcgcggtga ggcagtcgtc gaagagcgcg gccgtcgtac
    10261 ggtatccgct cacgccgagg ctgagcttct cgcccggcgt cagatggtcg gcgacgtacg
    10321 aggccgggga ggcgaccgtg ccggtcacga gccgcagaac ggcggcgggg aacacgcggc
    10381 gccgttcctc cggggtgctg aggacgacga ggtcgacggc gccgccccgc agcgcaaggc
    10441 tcggataacc gcgtacgacg ttgtaccggc cgcccggcgc gggcaggcgg gtgtcgatcg
    10501 tctgcggcac ctcgtcgaaa tcccacgacg tgatgccgga gcgctcgatg ccgttcggcg
    10561 tggcgctcgc gcgaccgtcg ccgcttaggg gggcaccgcg gccgtcgccc gaacctcggc
    10621 ggccggccgc gtcccgcgcg gccaccgcgg ggccccgccc cggtcgggcc ggcgccgggc
    10681 cggtcgagag aggacgcggc gcgacggccc gtgcgacgct gcgtcgggcc cgctcgctaa
    10741 ggcgctgctg caacgcggcc agatccttgt cgcggccggc cacccggcct tcgccgtcta
    10801 ccgcgcagaa cgacatcctg aggtgtggcg gcacacgctc ccacgagaaa tcgccggccg
    10861 cggcgagcgt gcccgtcagc cgccggatgc tcgccgcggc cgcctcggtg aggctgcggc
    10921 cctccggcgg cgtctcgggg aggtctgcca gaaccttcgc cgcccagtcg gcggcgggca
    10981 cgacgtggcg acgcagcgct ttcggcagtg atttgagaag cgcggtgacg agatcggggc
    11041 gaaggccggg aacctgccag gtgaacgcct ccggccgaag ccttgccaaa agcggcatcg
    11101 gcacgagcac gctgaccccg tcgttgtcgg agcccggcgc gaaatggtat tccagggcga
    11161 aggtctggtc gccttgccgc caggtctccg ggtaggcgtc tgtatcgggg tcgaccgtct
    11221 cgccccattc gtcggccgcc atggtgagga ggccggggtt ttctggccgg gtgcggttcc
    11281 accaggcctc gaagtcgcgc actcccgtaa cctctggcgg aatgcgggcg tcgtagaagt
    11341 cgaagacggc ctcgtcgtcg tacagcacgt ctcgcctgcg ggtgcgttcc tcccgctcgg
    11401 ccagccggcc ccgcagccgg tcgttggcac gcaagaagtc gtaggtgtgc ccgccgtggg
    11461 gatcgaattc acccgcaacg agagcgtggc gtagaaacag ctcccggctg agggcctggt
    11521 cgatcggcgc gtagcgcacc cgccgtcggg tgacgatggg cacgccatag agcgtgaccg
    11581 tctcgtacgc gacggcctgg ccctggcgct tctcccaatg tggctcgccg taggcgcgct
    11641 tcacgaggtc tccggcgagg cgttcggccc actgcgggtc gatcgccgcg ttcatccggg
    11701 catagagcct gctcgtctcc acgagctcgg ccgacatcac ggcggcgggt ggcttcttcg
    11761 ccaacgccga cgaggggtgc agtgtgaagc gcgtctgccg ggaaccgatg tagtccgacg
    11821 tgcggcggcg cggtgtggcg ccgcgtgggg agccgtggcg cttcctgctg gccgggctcg
    11881 aggccgcccg atcgtcgcgc aggccgatct gcgagagcag tcctgcaagg agtgcctggt
    11941 ggatgccgtg atcctcggtg ccctgcggcg cggtgctgtt cgtagcgtgg ccccgtcgca
    12001 gcgtcgcggt gagccgtttg agctgacggt cgacgtcttg ccattcccgc acccgcaggt
    12061 agttgagaaa ctcggccttg cacaggcgcc ggaacgcgct tgacgacagc tctttgcgct
    12121 tgtcgtgcag gtagttccag aggttcagca aggtgagaaa atcgctgtcg cggttcacga
    12181 agcgggcgtg tgcctggtcg gcggcggcgc ggcgctccat cggccgctcc ctggggtctt
    12241 gcaccgtgag cccggcgacg atcgcgcgca ccggccgggc gacgcccaga cgttccgcct
    12301 ccagcaccat gcggccgaac cggggctcga tcggcagcct cgcaaggctc cgcccggttg
    12361 gcgtgatgcg gcccgcgccg tcgatcgcgc cgagctcggc gagaaggccc aacccgtctt
    12421 tgacgccgcg gcggtcgggg ggcgtgagaa acgggaagtc ttcgatctcg ccgagaccga
    12481 gcgcgagcat ctgcaagatg acggaggcga ggttggtgcg cagaatctcg ggttctgtgt
    12541 actcgctgcg gccggcgtag tcctcttcgg aatacaggcg gatggcgatg ccggagcttt
    12601 ggcgcccgga acgcccggaa cgttgcttcg cggatgcctg ggagatcggc tcgatcggca
    12661 ggcgctgcac cttcgcgcgc gccgagtagc gggagatgcg tgccatgccc gcgtcgatga
    12721 cgtagcggat gccgggcacg gtgaggctcg tctccgccac gttcgtcgcc aagacgatgc
    12781 gacggcgcac cccgggcgcc ggcctctgga acacccggtg ctggtcggcg gccgagagcc
    12841 tgccgtagag gggcaggacc tctgtgcgag agtcgcccgc gtgcgacaag gcggcccgga
    12901 tcgcatcggc ggcgtcccgg atctctgcct cgccggagag gaagacgagc acatcgcccg
    12961 gtttctctcg gtcgagttcg gccagcgcgg cgaggatcgc gtcgttgacg tcgtcgtcgc
    13021 cgtcgcccgg ccgcgccggc gcccgccgtc cggctgcggc tccccgtgag cccgcgccgt
    13081 cggcgtcgcc tcgcgccccc gagtcgcctc gtgccgccgg ttgcgtgccc cgttgcccgg
    13141 tcgtcttccc gtctgcggtg tcgacccgcg ggcggtagcg gatctcgacg ggaaacgtgc
    13201 gtcccgacac ctcgacgacc ggcgccggct ccccggccga cgcgaaatgc cgggcgaagc
    13261 tttccgggtc gatcgtcgcc gacgtgacga tgaccttcag gtcggggcgg cgggggagga
    13321 tccgcttcag gtagccgagt aagaagtcga tcgtcaggct gcgctcgtgc gcctcgtcga
    13381 tgatgatcgt gtcgtaggcg cgtagcagcc ggtcgtggtg catctggttc aagaggatgc
    13441 cgtcggtcat cagcttgatc ctggtctctg gcccggcatg gtcggtgaag cgcacctggt
    13501 agccgacgag gccgccgacc tcgacgccga gttcgtcggc gatccgttcg gcgatcgtgc
    13561 gggctgcgag ccggcgcggc tgggtgtggc cgatggagcg acgccccatc tccaggcaga
    13621 tcttcggcag ctgcgtcgtc ttgccggagc ccgtctcgcc cgcgacgatg acgacctggt
    13681 tgtcgcggat gaccttggcg atctcctccc ggcgggcgct caccggcagg tcgggcgggt
    13741 acgagatcac gagtggcggt gccgcttgag gcagggtcgg tggggtggac atcagccctt
    13801 catcgtatct ggcgcaccgc caacggggcg tcgggcccgt agcctggggg agtgaccaac
    13861 agactcgccg aagagctcag cccctatttg cgtgcacacg ccgataaccc cgttgactgg
    13921 tatccgtggg gggcggaggc gttcgccgag gcgcgccgac gagatgtgcc gatcctgctt
    13981 tcgatcggct acgccgcctg ccactggtgt catgtga
//
LOCUS       DASTNL010000057        19224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_968776, whole genome shotgun sequence.
ACCESSION   DASTNL010000057 DASTNL010000000
VERSION     DASTNL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968776"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..2746)
                     /locus_tag="VFQ96_01940"
     CDS             complement(227..2746)
                     /locus_tag="VFQ96_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEU0256583.1"
                     /translation="MSLLDELPHPFDADAFFEAFSAWAARGGLELYPAQEEAMIGLVS
                     DAHVILSTPTGTGKSLVALGAHALALAQGRRSAYTAPIKALVSEKFFALVDTFGADRV
                     GMVTGDTHVNTDAPILCCTAEILANQALRHGPQTPFSVVVMDEFHYYGDPERGWAWQV
                     PLLEMTGSQFLLMSATLGDTTAIAADLSSRTGRETVSVTGVERPVPLSYRYVATPVHE
                     TVRALLDEALAPLYLVHFSQAAAVEAAQALAGAGVATRAQRDGIAEAIGAFRFQTGFG
                     RTLSRLLRLGVGVHHAGMLPGYRRLVEQLAQKGLLPVICGTDTLGVGINVPIRTVVLT
                     ALTKFDGVRTRRLRAREFHQIAGRAGRAGFDDAGDVVALAPEHEIENQKALAKAAGDP
                     KKKHKITRKKPPAGFVSWNEATFTKLIEAQPEELVPRLRMTHAMVINVIARGGDVYRH
                     LHSLVFDNHEPWRRQLALARRALGIYRSLLTGGVVEKTVAGPRLTLDLQPDFALNQPL
                     SPFALAAFELLDPASESHAVDVVSIIESILDDPRAVLAAQQYKARGQALAEMKAEGTE
                     YEERMERLEAITWPKPLDELIGQAFATYSQSQPWAKDFEPSPKSVVRDMHENAMTFAE
                     YVSWYGLTRSEGVLLRYLSDAYRALRQSVPPALTQRDPALADLVAWLGELVRQVDSSL
                     VDEWEELAHPDAAGELRALPRRPDAPLSRPAPVTANRHAFTVLLRNAMFRLVLLASRG
                     RLVELHDAAAPGGPDAAGWQGMLAAYGEEHDEILTGAPARGPQMLQIDTSTPGLWRLR
                     QVVDDPEGDRDWAIEAEADLAASDEAGEPVLRVAGLVRLDR"
     gene            complement(2844..3254)
                     /locus_tag="VFQ96_01945"
     CDS             complement(2844..3254)
                     /locus_tag="VFQ96_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HEU0256584.1"
                     /translation="MIDRQHAQELREAGLLWRHPAAGDRFTMVAEEFAGEVFTVSEMT
                     VESHEFTGGTILGFNGTTEWALDSVALDDALWMPRDDQLRGLLGRAFQSLVLKEGLFT
                     ATVLLSGAPRGFTAPDASTAYAEALLALIRDARG"
     gene            complement(3308..4336)
                     /locus_tag="VFQ96_01950"
     CDS             complement(3308..4336)
                     /locus_tag="VFQ96_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU0256585.1"
                     /translation="MKIGILTSGGDCPGLNAVIRGAVLKGDRVYGDEFVGFRYGWRGV
                     VDADFVPLDRRGVRGLSRQGGTILGSSRTNPFEGEHGGPENIQRMLDDNGIDAVIAIG
                     GEGTLTAARRLTDAGLRIVGVPKTIDNDLAATDYSFGFDTAVSIATEAIDRLRTTAES
                     HQRCMVVEVMGRNVGWIALHSGMAGGAHAILIPEEPQSVDQICRWVEHVRDRGRAPMV
                     VVSEGFHLDTMEGAHSHKGLDAFNRPRLGGIGELLAPLIEERTGIESRATVIGYVQRG
                     GAPSAYDRVLATRLGLAAVDAVEEGHWGSMVSLRGTEIATVSIAEATGGLNRVPKGRY
                     EEAALLFG"
     gene            complement(4412..5062)
                     /locus_tag="VFQ96_01955"
     CDS             complement(4412..5062)
                     /locus_tag="VFQ96_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU0256586.1"
                     /translation="MAHRIQVEVERKYDAGAQAPVPDLVGVGAVAVVEPATEALLVAV
                     YYDTEHLDVSAARRALRRRVGGADEGWHVKVAPEGSEGRTEHHWPLTDGEDVPAEIAE
                     ELAPLTAGRTLRPVARVVNRRVTTRLLDAAGYPVAEFCDDHVVAEDLRDGTTRRWREW
                     EVELAPAAPEHPAARAALWNGIEGRVARAAGRPSSSSSKLARALGIDPMPEDTPMS"
     gene            complement(5107..7206)
                     /locus_tag="VFQ96_01960"
     CDS             complement(5107..7206)
                     /locus_tag="VFQ96_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0256587.1"
                     /translation="MRHANNDGIIPVLARRVREVEAKVTAGKRLGPTNRTKFQVIATL
                     MREERARAKTDTALGDAGRAEQLKRLDGIATILAKIAARDTSLIALLENTSVSSETAQ
                     RMRRDWLVESGTELSPDDLIISTSAPRIAGETVLSPELAGRQVIPPSVRSRALSNPFL
                     PPDFAAVEAVHPRRRLDSWELLGPLYKAFERGSGGERASMTLPPAPRIDRFSPRGSRL
                     MNHQLRLIQSVREGHRTFLLADEPGLGKTAQALLAASVAGAYPMLAVVPNVVKMNWAR
                     EVEKWTPQRRVAVVHGDGADVDAFADIVVINYEILDRHLEWLSRLGFGGMVVDEAHFI
                     KNLHSQRSQYVLGLADSIRKRAHGHEPLLMALTGTPLINDIDDFRATWQFLGWLDGDR
                     PAPRLLDRLEETGLTPADPAFYAEARTAVIDLGMVRRRKIDVAADLPGRRVVDLPVEL
                     DGEAGRSIAQAERNLAQRLVERYRAAAASLGALGAERRERLIRTVAQAELEDSNARGD
                     GQNVFAMVRKIGQAKAHLAADYAIQLARSVGKVVFFAKHIDVMNEAERAFADAGLRSI
                     SVRGEQSARTRQAGIDAFANDPAVSLAVCSLTAAGVGLNLQAASNVVLAELSWTAAEQ
                     TQAIDRVHRIGQAEPVTAWRIVAAQTIDAKIAELIDQKQSLAARALDGSDVTVGSGDS
                     VQLDALVGVLRQALHLG"
     gene            complement(7567..7911)
                     /locus_tag="VFQ96_01965"
     CDS             complement(7567..7911)
                     /locus_tag="VFQ96_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU0256588.1"
                     /translation="MTVRIKRVYEAAEAGDGYRILVDRIWPRGLTKEAARVDEWLKEA
                     GPSTQLRKWFGHVPERFEEFAARYRDELRGSEALGRLRLATTEHDVVTLVYSAKDTVH
                     NQAVVLREELTA"
     gene            complement(7966..8331)
                     /locus_tag="VFQ96_01970"
     CDS             complement(7966..8331)
                     /locus_tag="VFQ96_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6804 family protein"
                     /protein_id="HEU0256589.1"
                     /translation="MKDDRYGSAEFSRAAVAPGLLGAIALLAGLALIGGSWFIGVQYA
                     ACLFACILCIIAGQAKVWWWLIGLVPIVVVWNPVFPLTLGEEWWRLAGIAGSAVFVAA
                     GLFIHSPLPENTPPRRRRR"
     gene            complement(8408..9262)
                     /locus_tag="VFQ96_01975"
     CDS             complement(8408..9262)
                     /locus_tag="VFQ96_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0256590.1"
                     /translation="MSRLAVPKTYKLFVAGAFRRSESGRTYPVVDARGTFAANVPAAS
                     RKDLRDAVQASRRAFPAWSGTTAYNRGQVLYRIAEVMEGRRAQFADEVRRTSGVGRAR
                     AEEEVDAAIDLWVWYAGWSDKLVQVAGGANPVAGPFFNLSVTEPTGVAAIVAPQESGL
                     VGLVSVVAPALVAGNTVVAMAARRHPFAAVGLAEVLATSDVPCGTVNILTGDPGELMP
                     VAAAHADVNALDLTGAGALEWVDAQIAAAETLKRVLTPVPGPATRSLQRILAFTETKT
                     IWHTTSTR"
     gene            complement(9259..10857)
                     /locus_tag="VFQ96_01980"
     CDS             complement(9259..10857)
                     /locus_tag="VFQ96_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0256591.1"
                     /translation="MSVTEGFTDYAPAPQARSLVTLRDEYGLFVDGRFVRGHGTPIEA
                     IAPASGERLATVTTASPADIDLAVAAARRAYEGVWSSMTGSRRGRYLYRIARIVQERS
                     RELAVAESLDNGKPIKETRDVDVPLVAAWFFYYAGWADKLDYAGLGSSPRPLGVAAQV
                     IPWNFPLLMLSWKIAPALAAGNTVVLKPAETTSLTALLFAEILQQAELPAGVVNIVAG
                     AGDAGRTLVEHPGVDKIAFTGSTAVGRQIARSVAGTDKRLTLELGGKAANIVFDDAPI
                     DQAIEGIVNGIFFNQGEVCSAGSRLIVQESVADEVVERLKRRLATLRMGDPLDKNTDI
                     GAINSAEQLERITRLCAVGEAEGATRWTSDVAVPEAGFWFPPTIFTGVAPAHRIAREE
                     IFGPVLSVLTFRTPAEAIAKANNTPYGLSAGIWTDKGSRMLAVADRLRAGVVWVNTFN
                     RFDPSSPFGGYKESGYGREGGRHGLAAYLAPGGDMEAGVRSGEGRALARSRSAAENSD
                     EGSVSRAAVELAGGGKGKEKGAHR"
     gene            complement(10916..11935)
                     /gene="deoC"
                     /locus_tag="VFQ96_01985"
     CDS             complement(10916..11935)
                     /gene="deoC"
                     /locus_tag="VFQ96_01985"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU0256592.1"
                     /translation="MIEVTDLRDAALRPARRAIDVLGTDAGEAALARYLRGIPGVDAV
                     GLEQRAASLAGRSIKTTSKARALDLVISLVDLTTLEGADTPGKVRDLAAKALRPDPSD
                     RTAPMVAAVCVYSDMVPYAVEALGAGHDGTHRAGVAVASVATAFPSGRASRAVKLAQV
                     AEAVEAGADEIDMVIDRGAFLAGRFGQVFDEIVAVRETCRRPDGGRAHLKVILETGEL
                     ATYDNVRRASWLAILAGADFIKTSTGKVSPAATLPVTLCMLEVVRDWYRMSGERVGVK
                     PAGGIRTAKDAIRYLVTVAETAGEEWLTPDLFRIGASSLVGDVLMQRQKIALGRYSGP
                     DYVTV"
     gene            complement(11932..12912)
                     /locus_tag="VFQ96_01990"
     CDS             complement(11932..12912)
                     /locus_tag="VFQ96_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU0256593.1"
                     /translation="MSDTDEILAVKAAELYYEAGKTQDEIGVALNISRWKVGRLLVQA
                     RERGFVRIEIVHPSARRFPLERELRGRFGLSDAVVVPVLDSDVQARVAEAAAEYLTTL
                     RPVPRSLGVSWGRTLQAVAARLRPGWAAGVNPVQINGSVSHNDGAAQDDTAVTIARKA
                     QGTATLLPSPAIFEHAETRRAVEADRSVQRVLALARSAAAYLFSAGVADQRSVHVSSG
                     YLTDREVADLAARGAVGDVVGRFITATGEVADPALDERTLGITLDELRRSRRTIAVIA
                     GQAKHAVCRAVVTSGLCSTLVTDEATATMLLESAPEGSGGLEKISKEGSS"
     gene            complement(13033..13965)
                     /locus_tag="VFQ96_01995"
     CDS             complement(13033..13965)
                     /locus_tag="VFQ96_01995"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0256594.1"
                     /translation="MRVYRGLESLPAGLPPSAVTIGKFDGVHRGHRSVIDAVVAAARR
                     DGLTATAVTFDRNPLRLLAPEKCPADLTGPEQKVALLGETGLDATLVLTFDHGLAATS
                     AEDFVADVLVGSLGMRRVLVGADFRFGARNAGSVETLRSLAPRHGYEVEVIDDVCRSG
                     GRRISSSWIRELLAAGDVEAAAQLLGRLPAVTGEVVHGLERGRTLGFPTANLSADSEG
                     MIPADGVYAGWLGLGGRRYPSAISVGDNPTFGDVPAKQVEAHVVDADLDLYGKRVRVE
                     FAHRIRGMVAFAGPEALALRIRDDVDETRALLAR"
     gene            complement(13992..14468)
                     /locus_tag="VFQ96_02000"
     CDS             complement(13992..14468)
                     /locus_tag="VFQ96_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256595.1"
                     /translation="MTFLAKSTPKPPRVQGAGRVLILVYGILALGATGRSVYQLSERF
                     AEAPLAYSLSALSAVVYIVATIALIAPGRAWYRVAWSTISFELTGVVVIGTISTFAPG
                     LLGLDGVDPFGQQSTVWSVYGAGYVFIPLVLPVLGMLWLHRHHPAGAVLAKAPGGA"
     gene            complement(14502..14870)
                     /locus_tag="VFQ96_02005"
     CDS             complement(14502..14870)
                     /locus_tag="VFQ96_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256596.1"
                     /translation="MIGWFTIVVVTIATLSGVLCVVLGLIGKAPNDLSMGGTALVALL
                     MFAQLVITIVAPFAGNHPTGSLFEFYLYLVSAVLLPFAGGLWALMDRTRWSTVVLGAI
                     CLAIAVMMVRMSIIWNVQGA"
     gene            complement(14867..15841)
                     /gene="truB"
                     /locus_tag="VFQ96_02010"
     CDS             complement(14867..15841)
                     /gene="truB"
                     /locus_tag="VFQ96_02010"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU0256597.1"
                     /translation="MGERAEPGSVRGLLIVDKPAGPTSHGVVARIRRLAGTRKVGHAG
                     TLDPMATGLLIVGLGPATRLLTFLVGLGKVYRATLRLGWETVTDDAEGQACGPPADAA
                     RLAGLSDDEIRAALRAFVGTIRQVPSSVSAVKVAGRRAYDVVRAGGVVELPARTVTIE
                     RLDVETIRRPAGAGGAAPAVDVTLEVACSSGTYIRALARDAGRALGVGGHLTALRRSV
                     VGPFGLGEAVGLTRLEADGVAPHLVCPAAAAARLFPTRLLDASQARDLGHGKRLSAAA
                     VAGGGDADAAGRFAAIAPDGRLVGIAEARDGVVRSLVNIAPGIEGAQQ"
     gene            complement(15884..17128)
                     /locus_tag="VFQ96_02015"
     CDS             complement(15884..17128)
                     /locus_tag="VFQ96_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0256598.1"
                     /translation="MPIFHVAAEAATYTQADTLWLMVATILALLMAPGVAFFYGGLVR
                     VTSVVSMMMMSFGAMAVVGVLWVLIGYGVAFGSPLIPHWIGDPFTDLGLSGIIGPGGT
                     VDIHLVAVAAFQAAFAIDVVALISGAIADRAKFGSWLIFAAVWSILVYFPVAHWIFDP
                     ADGWAAQMGVNDFAGGVAVHVNAGAAALALALVLGKRNGFGKGLDKPHNVPLTMLGAG
                     LLWIGWFGFNAGSAGAVDGTALLAFINTLVAPAASILAWLAVEKIKDGKPTSVGAASG
                     LVAGLVAAAPSCNVLTPGWTLVLGAIAGAVCALVADLRYKIGIDDSLNVVGLHLVGGV
                     IGTLFVGVAGIGIGFLATGSLVQFGVQTLVTVVVMCYSFGMAWLIGTGIERTVGFRLA
                     PDEAGTAPDVAIHGEEAYTLQA"
     gene            17267..18259
                     /locus_tag="VFQ96_02020"
     CDS             17267..18259
                     /locus_tag="VFQ96_02020"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0256599.1"
                     /translation="MRVGVIGAGAIGGTIAALLADAGHEVWVTARGAHAAAIRRTGLV
                     VEGAWGTHRPRVRIARELPAGCEVVFVCTKANDARRALEGSRRALAGACVVIVQNGLH
                     GTAEAARLLPGTGVVGALALFAATIAEPGRIVVTAPGDLWVGVVAGDPSAAQKVAALL
                     GQALPSHAIDDLAACQWTKLMINQVNAMPAITGLSVQETIARPRLRRIIARSMREATR
                     LAFATGVRFGRLQGLGPGILRLVSRGPRAAAGALLLAMARRMGDVPNLGSTLQSIRRG
                     RPSEVDALNGAVVAWAKEHGRAAPVNEALAAMVHEVEASGRFLTADEVARRFTR"
     gene            complement(18285..18959)
                     /locus_tag="VFQ96_02025"
     CDS             complement(18285..18959)
                     /locus_tag="VFQ96_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU0256600.1"
                     /translation="MELTTTPRVELWHALTDEITHNYSRGRIFLAVDGILGSGTAAFA
                     DGLADALRERGHETVRASLTDFAAPREARERQGADSGHGFYDDEYDEAAFRRLLVEPF
                     SLAGRPFHTASYDRAAERSLETAPQTAGDDAFCIVDGVFLNRPELHGIWHYSLYLEVP
                     WAVAYARLQAAGQAPSADPDDPANRRTRKGQDRYLAEVFPRGVANAIIDNTDDNRPLR
                     VFADSC"
     gene            19030..>19224
                     /locus_tag="VFQ96_02030"
     CDS             19030..>19224
                     /locus_tag="VFQ96_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0256601.1"
                     /translation="MDDAARTRFVRSLTRWYAGSARDLPWRRPGFSPWGVLVSEIMLQ
                     QTPVARVIPRLEQWLTRWPTP"
BASE COUNT         2892 a         6701 c         6890 g         2741 t
ORIGIN      
        1 gccatcgcgt tcgcggtgtc gtggaagccg ttggtgaagt cgaagaacag tgcgaggacc
       61 acaacgagca cgacgatcgt ggtcacgtgc acgggcatcc ctcgaatggt cgacatccct
      121 gcccgcgacg gccgctgccc cgtgccccgc agggaccgca tcgccaggtg cggccacgtc
      181 gatactcaca cctcgcgcgg cgagggtcaa ctcggcatgc gaaccgctaa cgatccagcc
      241 gcacgaggcc ggcgacccgc aacaccggct cgccggcctc gtcggaggcg gccaggtcgg
      301 cctcggcctc gatcgcccaa tcccggtcgc cctccgggtc gtcgacgacc tgtcgcagcc
      361 gccacaggcc gggcgtcgac gtgtcgatct gcagcatctg cggcccgcgt gccggggcgc
      421 ccgtcaagat ctcgtcgtgc tcttcaccgt aggccgccag catgccctgc cagccggcgg
      481 cgtcggggcc gccgggggcc gcggcgtcgt gcagctcgac gagacggccg cgcgaagcca
      541 acagcacgag gcggaacatc gcgttgcgca agagcaccgt gaaggcgtgc ctgttcgcgg
      601 tgaccggggc cggccgtgac aagggcgcgt caggtcggcg cggcaaggcc cgcagctccc
      661 cggcggcgtc ggggtgggcg agctcttccc actcgtcgac gaggctggag tcgacctggc
      721 gcaccaactc ccccagccac gcgacgaggt cggcgagcgc cgggtctcgt tgcgtgagcg
      781 cgggcggcac gctctggcga agcgcccgat aggcgtcgga gagatagcgc aagaggacgc
      841 cttcggaccg ggtcagcccg taccaggaca cgtattccgc gaaggtcatc gcattttcgt
      901 gcatgtcgcg cacgacagac ttcgggctcg gctcgaaatc ctttgcccac ggctggctct
      961 ggctgtaggt ggcgaaggcc tggccgatga gctcgtcgag gggtttcggc caggtgatcg
     1021 cctcgaggcg ctccattcgc tcctcgtact cggttccctc ggctttcatc tcggccagag
     1081 cctggccgcg ggccttgtac tgttgggcgg cgagcaccgc ccgcgggtcg tcgaggatcg
     1141 actcgatgat cgagacgaca tccaccgcgt ggctctcgct cgccgggtcg agcagctcga
     1201 aggcggcgag ggcgaagggc gagagcggct ggttcaaggc gaagtcgggc tgcaggtcga
     1261 gcgtcaaccg gggcccggcg acggtcttct cgacgacccc gcccgtgaga agagagcggt
     1321 agatgccgag ggcgcggcgg gcgagcgcga gctgacggcg ccacggctcg tggttgtcga
     1381 acaccaggga atgcaggtgc cggtagacgt cgccgccacg ggcgatcacg ttgatgacca
     1441 tcgcgtgggt catccggagg cgcggcacga gctcctccgg ctgggcctcg atgagcttcg
     1501 tgaaggtcgc ctcgttccac gagacgaacc cggcgggcgg cttcttgcgc gtgatcttgt
     1561 gcttcttctt cgggtcgccc gccgccttgg cgagcgcctt ctggttctcg atctcgtgct
     1621 ccggggcgag ggcgacgacg tcgccggcgt catcgaaccc ggcccggccg gcgcggcccg
     1681 cgatctggtg gaactcccgg gcccgaaggc ggcgcgtccg cacgccgtcg aacttcgtca
     1741 gtgccgtcag gacgaccgtg cggatcggca cgttgatgcc gacgccgagc gtgtcggtgc
     1801 cgcagatcac aggcaggagc cctttctggg ccagctgttc cacgaggcgg cgataccccg
     1861 gcagcatgcc cgcgtggtgc acgccgacgc cgaggcgcag aagccgcgag agggtgcgcc
     1921 cgaacccggt ctggaaccgg aaagctccga tcgcctccgc gatgccgtct cgctgcgccc
     1981 tcgtcgcgac gccggcgccg gccagcgcct gggccgcctc gacggccgcg gcctgcgaga
     2041 aatgcacgag gtagaggggc gccaaagcct cgtcgaggag ggcgcgcacg gtctcgtgca
     2101 ccggggtggc gacgtagcgg tagctgagcg gcacgggccg ctccaccccc gtcaccgaca
     2161 cggtctcgcg tccggtacgg ctcgagaggt ctgctgcgat ggcggtcgtg tcgccgagcg
     2221 tcgccgacat gaggaggaac tgggatcctg tcatctccag gagcgggacc tgccacgccc
     2281 agccgcgctc ggggtcgccg tagtagtgga actcgtccat gacgacgacc gagaacgggg
     2341 tctgcgggcc gtgacgcaag gcctggttgg cgaggatctc ggccgtgcag cacaggatcg
     2401 gcgcgtcggt gttcacgtgg gtgtcgccgg tgaccatgcc cacacggtcg gcgccgaacg
     2461 tgtcgacgag tgcgaagaac ttctcgctga cgagcgcctt gatgggagcc gtgtaggcgc
     2521 tgcgccggcc ctgggccagg gcgagggcgt gcgcgcccag cgcgacgagc gacttgcccg
     2581 ttcctgtagg tgtgctgaga ataacgtggg cgtcggagac gagcccgatc atcgcttctt
     2641 cctgggccgg gtagagttcg agccccccgc gcgcggccca ggccgagaac gcctcgaaga
     2701 aggcgtcggc gtcgaacgga tgaggcagct cgtcgagaag agacatgacc tctcgatcat
     2761 gccgcaccgg gcgtcagccg acccggcccc gccccggcat tcgacgaacc tgcgttgcga
     2821 gcgtgtggtc tggccggccc cgatcagccg cgcgcgtcgc ggatcaaggc gaggagggcc
     2881 tcggcgtagg ccgtgctcgc gtctggcgcg gtgaagcccc gcggcgcgcc ggagaggagc
     2941 accgtggcgg tgaagaggcc ttctttcaga acgagagact ggaaggcccg gccgaggagg
     3001 ccgcgtagct gatcgtcgcg cggcatccac agcgcgtcgt cgagcgccac cgaatcgagc
     3061 gcccactcgg tcgtgccgtt gaagcccaga atcgtgccgc cggtgaactc gtgagactcg
     3121 accgtcatct cgctgacagt gaagacctcg ccggcgaatt cctccgcgac catcgtgaac
     3181 cggtcgccgg cggcggggtg gcgccagagg agcccggcct cacgaagctc ctgggcgtgt
     3241 tgccgatcga tcatgccgcc tcccgggcta ccaccctacc ggggcgcccc gccgcctgac
     3301 cggcggatca gccgaagagc agtgccgcct cttcgtaacg ccctttcggc acccggttca
     3361 gcccgcccgt cgcctcggcg atgctcacgg tggcgatctc ggtgccccgc aacgagacca
     3421 tgctgcccca atgcccctct tcgaccgcgt cgacggcggc caggccgagt cgcgtggcca
     3481 acacgcggtc gtaggcgctc ggcgcaccgc cgcgctgcac gtagccgatg acagtggccc
     3541 gggattcgat gccggtacgc tcctcgatga ggggtgcgag cagctccccg ataccgccca
     3601 ggcgcggcct gttgaaggcg tcgaggccct tgtgggagtg tgcaccctcc atcgtgtcga
     3661 ggtggaagcc ctccgacacg acgaccatgg gcgccctgcc gcggtcgcgc acgtgttcca
     3721 cccatcggca gatctggtcg acggactgcg gctcctcggg gatcaagatg gcgtgcgcgc
     3781 cgcccgccat gccggaatgc agggcgatcc agccgacgtt gcgccccatg acctccacga
     3841 ccatgcagcg ctgatgcgat tcggcggtgg tgcgcagccg gtcgatcgcc tccgtcgcga
     3901 tcgagaccgc cgtgtcgaag ccgaacgagt agtcggtggc ggccaggtcg ttgtcgatcg
     3961 tcttcggcac cccgacgatg cgtaacccgg cgtcggtcag ccggcgcgcc gccgtgagcg
     4021 tgccctctcc gccgatggcg atgacggcgt cgatgccgtt gtcgtcgagc atccgctgga
     4081 tgttctccgg accgccatgc tcgccctcga acgggttggt gcggctggag ccgaggatcg
     4141 tgccgccctg ccgggacagg ccgcgcacgc cgcggcgatc gagcggcacg aagtcggcgt
     4201 cgacgacgcc gcgccagccg taccggaacc cgacgaactc gtcgccgtag acccggtcac
     4261 ccttcaagac ggcgccgcgg atgacggcgt tgagccccgg acagtcgccg ccggaggtca
     4321 gaatcccgat tttcatgcag cccgctttct gctgtccgtg tcattgcggc gaggccgaac
     4381 gccatgccga gcctagaacg ccgggccgga atcacgacat cggcgtgtct tcgggcatcg
     4441 ggtcgatgcc gagcgcccgg gcgagcttcg acgagctcga cgacggccgg cctgcggcgc
     4501 gggcgacgcg gccctcgata ccgttccaga gcgccgcccg ggcggccgga tgctcggggg
     4561 cggcgggcgc gagctcgacc tcccattccc gccaacgacg ggtcgtgccg tcgcgcaggt
     4621 cttcggcgac gacgtggtcg tcgcagaact ccgccacggg gtagccggcg gcatccagca
     4681 ggcgggtcgt gacgcgccgg ttcacgacgc gggcgacggg gcgaagcgta cgaccggcgg
     4741 tgagcggggc gagttcctcc gcgatctcgg cggggacatc ctcgccgtcg gtcaagggcc
     4801 agtggtgctc ggtgcgcccc tcggagcctt cgggggcgac cttcacgtgc cagccttcgt
     4861 cggcgccgcc gacccggcgg cgcagggcgc gccgggcggc actcacgtcg aggtgctcgg
     4921 tgtcgtaata gacggcgacc aacagggcct cggtcgcggg ctcgacgacc gcgacggcac
     4981 cgacaccgac gaggtcgggc acgggagcct gggcgccggc gtcgtacttg cgttcgacct
     5041 caacctggat ccggtgcgcc atacctcttg tctagcgcag gatcgccgcc caggcgaatc
     5101 ggagcctcag ccaaggtgca gggcttggcg cagcacgccg accagggcgt ccagttgcac
     5161 cgaatcgccg gagccgaccg tgacgtcgga gccgtcgagc gcccgcgccg cgagtgactg
     5221 cttctggtcg atgagctccg cgatcttcgc gtcgatcgtc tgggcggcga cgatgcgcca
     5281 cgcggtgacg ggttcggcct ggccgatccg gtgcacccgg tcgatggcct gggtctgctc
     5341 cgccgcggtc cacgagagct cggcgaggac gacgttggag gcggcctgaa ggttcagccc
     5401 gaccccggcg gcggtgaggg agcagacggc cagcgagacg gccggatcgt tcgcgaacgc
     5461 atcgatgccg gcctgacgcg tgcgggcgct ctgctcgcca cgcaccgaga tgctcctaag
     5521 gcccgcgtcg gcgaacgccc gctcggcctc gttcatcacg tcgatgtgct tggcgaagaa
     5581 gaccaccttg ccgacggagc gcgcgagttg gatcgcgtag tcggcggcga gatgcgcctt
     5641 cgcctggccg atcttgcgca ccatcgcgaa gacgttctgg ccgtcgccgc gcgcgttgga
     5701 atcctccagc tcggcctgcg ccaccgtgcg gatgaggcgc tctcgccgtt cggccccgag
     5761 ggcgccgaga gaggccgcgg cggcccggta acgctccacg agccgctggg cgagattccg
     5821 ctcggcctgc gcgatggagc ggccggcctc gccgtcgagc tccaccggga ggtcgacgac
     5881 acggcggccc ggcaggtcgg cggcgacgtc gatctttcgc cgccgcacca tgccgaggtc
     5941 gatgacggcg gtgcgcgcct cggcatagaa cgccgggtcg gcgggggtga gccccgtctc
     6001 ctccaaccgg tcgaggaggc gaggagccgg gcgatccccg tcgagccagc cgaggaactg
     6061 ccaggtggcg cggaagtcgt cgatgtcgtt gatgagcggc gtgcccgtca acgccatgag
     6121 cagcggctca tggccgtgcg cacgcttacg gatcgagtcg gcgaggccca acacgtattg
     6181 ggagcgctgg gagtgcaggt tcttgatgaa atgcgcctcg tcgacgacca tgccgccgaa
     6241 cccgagccgg gagagccatt cgagatgccg gtcgaggatc tcatagttga tgacgacgat
     6301 gtcggcgaag gcgtcgacgt cggcgccatc gccgtggacc actgcgacgc gccgctgcgg
     6361 cgtccacttc tcgacctcgc gtgcccagtt catcttgacg acgttcggca cgacggcaag
     6421 catcgggtag gcgcctgcca ccgacgccgc gaggagcgcc tgcgcggtct tgccgaggcc
     6481 cggctcgtcg gcgagcaaga acgtgcgatg cccttctcgc acactctgga tgaggcgcag
     6541 ctggtggttc atcagcctgc tgccccgcgg cgagaagcgg tcgatgcgtg gcgccggcgg
     6601 gagcgtcatg ctggcccgtt cgccgcccga gccgcgttcg aaggccttgt agagcggccc
     6661 gagcagttcc cacgaatcga ggcggcgacg cgggtgcacc gcctcgacgg ccgcgaagtc
     6721 ggggggcagg aacgggttcg acaacgcccg cgagcgcact gacggtggaa tgacctgccg
     6781 cccggcgagc tccgggctca gcaccgtctc gccggcgatc ctgggggcgc tcgtgctgat
     6841 gatgaggtcg tcggggctca actcggtgcc cgactccacc agccagtcgc gacgcatccg
     6901 ttgagcggtc tcgctggaga ccgacgtgtt ctcgagaagc gcgatgagcg acgtgtcgcg
     6961 ggccgcgatc ttcgcgagga tcgtcgcaat gccgtcgagg cgcttcagct gttcggcgcg
     7021 gccggcgtct ccgagcgccg tgtcggtctt cgcccgggca cgctcctcgc gcatcagggt
     7081 cgcgatcacc tggaacttcg tgcgattcgt aggacccaga cgcttgccgg ccgtgacctt
     7141 ggcctcgacc tcccgcaccc ggcgggccaa gacggggatt atcccgtcgt tgttggcatg
     7201 ccgcagtcgg gacgtgccgt ggtgaggcgg ccgggtctgc gtagctgcac ctgtgcgcgg
     7261 cactgatcct ccctaaggat gacgtcgatg gcgggtgacg acgacgggcg cgggcttctg
     7321 ccttacggtg gggtatccct gccgccggcc tgccgcgcgg gcgctcgacc aacgtcgcac
     7381 acgatcgttg gaggtgccca taccggccga cacgccccgg cgttcactca cctgccgcca
     7441 ccattgtagg gcacgccatg ccgctcgacg cgcgccgggc tgaacggcac gccggccgca
     7501 gacgaggcac cggcggcatt tccggcacga ggtgctacgg gcacgggcgc cctgtgtcgg
     7561 ccgccgtcac gccgtgagct cctcccgtaa gacgaccgcc tgattgtgca cggtgtcttt
     7621 ggcggagtac acgagagtca cgacgtcgtg ctcggtcgtc gccagacgca gccgacccaa
     7681 cgcctcgctc ccccgcaact cgtcacggta gcgggcggcg aactcttcga aacgctccgg
     7741 gacgtgcccg aaccacttgc gcagctgtgt cgaagggccg gcctccttca gccactcgtc
     7801 gacgcgagcg gcctctttcg tgagccccct gggccagatg cgatcgacga ggatgcgata
     7861 tccgtcgccc gcttcggccg cctcgtagac gcgtttgatg cgaacagtca tgtttccact
     7921 gtggcacggc acgcgcggcc gcgaggccgg cgcgacagag ggcccttacc gacgcctgcg
     7981 cctgggcggc gtgttctccg gcagcgggct gtggatgaaa aggccggccg ccacgaacac
     8041 cgccgagcct gcgatgccgg ccagacgcca ccattcctcg ccgagggtga gaggaaaaac
     8101 cggattccag acgacgacga tcggcacgag cccgatgagc caccaccaca ccttggcctg
     8161 gccggcgatg atgcacaaga tgcaggcgaa gaggcatgcc gcgtactgca ccccgatgaa
     8221 ccaagagccg ccgatcaacg ccaagccggc caggagcgcg atggcgccga gcagccccgg
     8281 agccaccgcg gcacgggaga actcggcgga gccgtatcgg tcgtctttca tcggtcttgc
     8341 cagcatacgc gaccggccgg tcgccgcagc ctcgcccggt tcacgggcgg gcgcgacgaa
     8401 cgtcgcgtca gcgcgtcgac gtcgtgtgcc agatggtctt cgtctcggtg aaagcgagga
     8461 tgcgctgcag gctccgcgtg gccgggccgg gcacgggtgt cagcacccgc ttcaacgtct
     8521 cggccgccgc gatctgcgca tccacccact ccagcgcccc ggcccccgtc aggtcgagcg
     8581 cgttaacgtc ggcgtgggcc gccgccaccg gcatgagctc gcccgggtcc ccggtgagga
     8641 tgttcacggt gccgcacggc acgtcgctcg tggcgagcac ctcggccagg ccgacggcgg
     8701 cgaacggatg acgcctcgcg gccatggcga cgacggtgtt gcccgccacg agcgcggggg
     8761 cgacgacgct gacgaggccc acgaggccgc tttcttgcgg tgcgacgatc gctgcgacgc
     8821 cggtgggctc cgtcacggag aggttgaaga acggcccggc gaccgggttc gccccgccgg
     8881 cgacctgcac gagcttgtcg ctccacccgg cgtaccagac ccacaggtcg atcgccgcat
     8941 cgacctcctc ttcggcccgc gcccggccga cgccgctcgt gcgccgcacc tcgtcggcga
     9001 actgggcccg gcgcccctcc atgacctctg cgatgcgata gagcacctga ccccggttgt
     9061 aggccgtggt gcccgaccag gccgggaagg ctcgcctcga cgcctgcacg gcgtcgcgca
     9121 ggtctttgcg ggaggcggcc ggcacgttcg cggcgaatgt gccgcgtgcg tcgacgacgg
     9181 ggtaggtgcg ccccgactcg ctgcgccgga aggcaccggc gacgaacagc ttgtacgtct
     9241 tcggcacggc aaggcggctc atcggtgcgc ccctttctct ttccctttac cgcccccggc
     9301 caactccaca gccgcccggg aaaccgatcc ctcgtccgag ttctcggccg cagatcgcga
     9361 ccgtgccaag gcccggccct cgcccgaccg gaccccggcc tccatatcgc cgcccggtgc
     9421 gagataggcg gcaaggccgt gtcggccgcc ctcccgccca taacccgact ccttgtaacc
     9481 gccgaacggg ctcgacgggt cgaaccggtt gaacgtgttc acccagacga caccggcgcg
     9541 cagccggtcg gcgacggcga gcatgcggct gcccttgtcg gtccagatgc cggcggagag
     9601 cccatacggg gtgttgttcg ccttggcgat cgcctcggcg ggggtgcgga acgtcaagac
     9661 ggagaggacc gggccgaaga tctcctcacg ggcgatgcgg tgggcggggg ccacgcctgt
     9721 gaagatagtc ggggggaacc agaagccggc ctcgggaacc gcgacgtccg aggtccaccg
     9781 cgtggcgccc tccgcctcgc ccaccgcgca cagccgcgtg atgcgctcca actgctcggc
     9841 ggagttgatc gccccgatgt cggtgttctt gtcgagcgga tcgcccatcc gcagcgtcgc
     9901 gagccggcgt ttcagccgct ccaccacctc gtcggccacc gattcctgca cgatcaaccg
     9961 gctgccggca ctgcacacct cgccttgatt gaagaagatg ccgttcacga tgccttcgat
    10021 ggcctggtcg atgggcgcgt cgtcgaagac gatgttcgcg gccttgccac ccagctcgag
    10081 cgtgagacgc ttgtcggtgc ccgcgacgga gcgcgcgatc tggcggccga ccgccgtcga
    10141 gcccgtgaag gcgatcttgt cgacgcccgg atgctcgaca agggtgcgcc cggcatcgcc
    10201 ggcgccggcc acgatgttga cgacgccggc gggcagttcc gcctgctgca ggatctcggc
    10261 gaagaggagc gccgtcagcg aggtcgtctc tgccggcttc aagacgaccg tgttgcccgc
    10321 cgcgagcgcg ggcgcgatct tccacgaaag catcagcagg ggaaagttcc acgggatgac
    10381 ctgcgccgcc acaccgagcg ggcggggcga agagcccaag ccggcatagt cgagcttgtc
    10441 ggcccagccg gcgtaataga agaaccaggc ggccaccagc ggcacgtcga cgtcgcgggt
    10501 ctctttgatc ggcttgccgt tgtccagact ttcggcgacc gccagctcgc ggctgcgctc
    10561 ctgcacgatg cgggcgatcc ggtacaggta gcggccccgg cgggagcccg tcatcgacga
    10621 ccacacgccc tcgtaggcgc gacgggccgc ggcgaccgcg aggtcgatgt cggcggggct
    10681 tgccgtcgtc acggtcgcga ggcgttcacc ggaggccggc gcgatcgcct cgatcggcgt
    10741 gccgtggccc cgcacaaaac ggccgtcgac gaagagcccg tactcgtccc gcagggtgac
    10801 gagggagcgc gcctgcgggg cgggcgcgta gtcggtgaag ccttcggtga cgctcatggc
    10861 aatcctgctc tcttagggtg ccagggccgg cctggcgggt tcgcgcctag cccggtcata
    10921 cggtgacgta gtcggggccg gagtaacggc cgagcgcgat cttctgtcgc tgcatgagca
    10981 cgtcgccgac gaggctggag gccccgatac ggaagaggtc gggcgtcagc cactcctcgc
    11041 cggcggtctc ggcgacagtg acgaggtagc ggatggcgtc tttggcggtg cggatgccgc
    11101 cggccggctt caccccgacg cgttcgcccg acatgcggta ccaatcgcgc accacctcga
    11161 gcatgcacag agtgaccggc agagtcgctg ccggcgagac ctttccggtc gaggtcttga
    11221 tgaagtcggc cccggcgagg atcgccagcc acgaggcgcg gcgcacgttg tcgtaggtcg
    11281 ccagttcccc cgtctcgaga atcaccttga ggtgggcgcg gccgccgtcg gggcggcggc
    11341 aggtctcacg caccgccacg atctcgtcga acacctggcc gaagcggccg gccagaaacg
    11401 cgccccggtc gatgaccatg tcgatctcgt cggcgccggc ctcgacggcc tcggccacct
    11461 gggcgagctt caccgcccga gaggcccgcc cgctcggaaa cgcggtcgcg acagaagcga
    11521 ccgcgacacc cgcgcggtgc gtgccgtcat gccccgcgcc gagggcctcg acggcgtagg
    11581 gcaccatgtc gctgtagacg cagacggccg ccaccatcgg ggcggtgcgg tccgacgggt
    11641 cgggcctcaa cgcctttgcc gcgaggtctc gcaccttgcc gggcgtgtcg gcgccctcca
    11701 acgtcgtcag gtcgacgagc gagatgacga ggtcgagggc ccgcgccttg gaggtggtct
    11761 tgatcgatcg gcccgccaga gacgcggcac gctgttcgag accgacggcg tcgaccccgg
    11821 gtatgccgcg caggtacctg gccaacgccg cctcgccggc atccgtgccc aagacgtcga
    11881 tcgcgcgccg ggccgggcgc agggcggcgt cacgaagatc cgtcacttcg atcatgagga
    11941 cccctccttc gagatcttct ccaggccccc tgagccttcg ggggccgatt ccagaagcat
    12001 cgtcgcggtg gcctcgtcgg tcaccagggt gctgcacagc ccgctcgtga cgacggcgcg
    12061 gcagaccgca tgcttggcct ggcccgcgat caccgcgatc gtgcgccggg agcgccgcag
    12121 ctcgtcgaga gtgatgccga gcgtgcgttc gtcgagcgcc gggtcggcga cctctccggt
    12181 cgccgtgatg aagcgaccca ccacgtcgcc caccgcgccg cgtgcggcga ggtcggccac
    12241 ctcacggtcg gtcaggtaac cgctggagac gtgcacggag cgttggtctg cgacacccgc
    12301 gctgaagagg tatgcggcgg cggagcgggc cagagccagc acgcgttgca cggaacggtc
    12361 ggcctcgacc gcccgcctgg tctcggcgtg ttcgaagatc gcggggctcg gcaggagggt
    12421 ggccgtgccc tgcgccttac gggcgatggt cacggccgtg tcgtcttggg cggcgccgtc
    12481 gttgtgcgag acgctgccgt tgatctgcac cgggttcacc ccggcagccc agccggggcg
    12541 caggcgggcc gcgaccgcct gcagcgtgcg cccccaactc accccgaggc tgcggggcac
    12601 ggggcgcagg gtcgtcaggt attcggcggc ggcctcggcg acacgagcct gcacgtcgga
    12661 gtcgagcacg ggaacgacga cggcgtcaga gagcccgaag cggccgcgca actcccgttc
    12721 cagcgggaac ctgcgggcgc tcggatgcac gatctcgata cgcacgaacc cgcgctcgcg
    12781 ggcctgcacc aagagccggc cgaccttcca gcggctgatg ttcagcgcga ccccgatctc
    12841 gtcctgcgtc ttgccggcct cgtaatagag ctcggcagcc ttgacggcga ggatctcgtc
    12901 ggtgtcgctc atcggcatcc gcccgtctcc tcccgtcggc gcggcgcata cagacggcac
    12961 cagcgcgcgt cttcagccta aggaatcgcg tggcaaagca ccacacatgt gcaaaggatg
    13021 ctcagatgag cattatcggg cgaggagtgc gcgcgtctcg tcgacgtcgt cgcggatgcg
    13081 cagggccagg gcctcgggcc cggcgaaggc gaccatgccc cgaatgcggt gtgcgaactc
    13141 gacccgcacc cgcttgccgt aaaggtcgag gtcggcgtcg acgacgtgcg cctccacctg
    13201 tttcgccggc acgtcgccga aggtcgggtt gtcgccgacc gagatggcac tcggataccg
    13261 ccggcccccg agaccgagcc aaccggcgta cacgccgtcg gccgggatca tgccctccga
    13321 gtcggcggaa aggttcgccg tcggaaagcc gagcgttctg ccgcgttcga gcccgtgcac
    13381 gacctcgccg gtcaccgccg gcagcctgcc caaaagctgc gccgccgcct cgacgtcgcc
    13441 cgcggccaga agctcgcgga tccagctgga agagatgcgg cgaccgccgc tgcggcagac
    13501 gtcgtcgatg acctccacct cgtagccgtg gcggggcgcg aggctgcgca gcgtctcgac
    13561 gctgccggcg ttgcgggcgc cgaagcggaa gtcggcaccg acgagcaccc ggcgcatgcc
    13621 gagcgagccg acgagcacgt cggcgacgaa gtcctccgcg ctcgtggccg ccaggccgtg
    13681 atcgaacgtc aagacgagcg tcgcgtcgag ccccgtctcc cccaagagcg cgaccttctg
    13741 ttccgggcct gtgaggtcgg cagggcattt ctcgggggcc agaagccgca acgggttgcg
    13801 gtcgaaggtg acggcggtcg ccgtgaggcc gtcgcgccgt gcggcggcga cgaccgcgtc
    13861 gatgaccgag cgatggcccc gatgcacccc gtcgaacttt ccgatcgtga ccgccgaggg
    13921 tggcagcccc gcaggcaaag actcaaggcc gcgatagacg cgcatctcgt cacggggtgc
    13981 cgggccgggc atcacgcccc tcccggcgcc ttggccagga ccgctccggc tgggtggtgc
    14041 cggtgcagcc acaacatgcc gagcaccggc aaaacgagcg ggatgaacac gtagccggcc
    14101 ccgtagaccg accacacggt cgactgctgg ccgaacgggt cgacgccgtc aagaccaaga
    14161 aggccggggg cgaaagtgct gatcgtcccg atgacgacga cccccgtcag ctcgaagctt
    14221 atggtcgacc aggcgacgcg gtaccacgcg cgaccggggg cgatcaaggc gatcgtggcg
    14281 acgatgtaga cgaccgcgga gagcgccgag aggctgtagg cgagcggcgc ctcggcgaaa
    14341 cgctccgaaa gctggtagac gctacgcccc gtcgcgccga gcgcaaggat gccatagacc
    14401 aggatgagga cgcgcccggc gccctgcaca cggggcggct tgggggtgct cttcgcgagg
    14461 aatgtcatga cgccttcgat tatcgcagcc gcgcgagccg ctcaggcgcc ctgcacgttc
    14521 cagatgatgc tcatgcgcac catcatgacg gcgatggcga ggcagatcgc acccaagacg
    14581 accgtgctcc accgggtgcg gtccatgagc gcccacaggc cgccggcgaa cggcagaagg
    14641 accgccgaga cgagatagag gtaaaactcg aaaagcgagc cggtcgggtg gttgcccgcg
    14701 aacggggcga cgatggtgat gacgagctgg gcgaacatga ggagcgcgac gagcgcggtg
    14761 ccgcccatcg acaggtcgtt cggtgccttg ccgatgaggc ccaggacgac gcaaagcacc
    14821 ccggagaggg ttgcgatcgt gacgacgacg atggtgaacc atccgatcac tgctgggctc
    14881 cttcgatgcc gggcgcgatg ttgacaaggc tgcgcacgac gccgtcgcgg gcctcggcga
    14941 tgccgacgag ccgaccgtct ggcgcgatcg ccgcgaaccg gcccgcggcg tcggcgtcgc
    15001 cgccgcctgc gacggccgcc gcggacagcc gtttgccgtg gccgaggtcg cgggcctgcg
    15061 aggcgtcgag gagccgggtc ggaaagagcc tggccgccgc tgcggccggg cagacgagat
    15121 gcggggcgac accgtcggcc tccaggcggg tgaggccgac ggcctcgccc aggccgaacg
    15181 gcccgaccac gctgcgccgc agggccgtca ggtgcccgcc cacgccgagg gcgcggcccg
    15241 cgtcgcgggc gagcgcgcga atataggttc ccgaggagca ggccacctcg agcgtcacgt
    15301 cgacggcggg ggctgcgccg cccgcacccg cagggcgccg aatcgtctcg acgtcgaggc
    15361 gttcgatcgt gaccgtgcga gccggaagct cgaccacgcc gcccgctcgc acgacgtcgt
    15421 aggcgcgccg gcctgcgacc ttgaccgcgc tgacgctgct cggaacctgc cggatcgtgc
    15481 cgacgaaggc gcgcagcgcg gcccggatct cgtcgtcgct caggccggcg agccgggccg
    15541 catccgccgg ggggccacag gcctgcccct ccgcgtcgtc ggtgaccgtc tcccacccga
    15601 ggcgcagagt ggcccgatag accttgccca gcccgacgag aaaggtcagg agcctcgtcg
    15661 ccgggccgag gccgacgatg aggaggccgg tcgccatggg gtcgagtgtg ccggcatgcc
    15721 ccaccttgcg cgtgccggcc agccggcgga tgcgggcgac cacaccgtgg ctggtcggcc
    15781 cggccggctt gtcgacgatg agcagccccc gcaccgatcc cggctccgcg cgctccccca
    15841 cggccgccat gctatcaacg ggctgcggcc ggatgcccgg ccctcaggcc tgcagggtgt
    15901 aggcctcttc gccgtggatg gccacatccg gcgcggtgcc cgcctcgtcg ggtgccagcc
    15961 ggaagccgac ggtgcgctcg atgccggtgc cgatcagcca ggccatgccg aacgagtagc
    16021 acatgacgac gaccgtgacg agggtctgca cgccgaactg cacgagggag ccggtggcga
    16081 ggaagccgat gccgatgccg gcgaccccaa cgaacagggt gccgatgacg ccgcccacca
    16141 ggtgcagccc gacgacgttc aggctgtcgt cgatgccgat cttgtaacgc aggtcggcaa
    16201 cgagggcgca gacggcgccg gcgatcgcgc ccagaacgag ggtccagccc ggcgtcaaca
    16261 cgttgcagga aggcgccgcg gcgacgaggc cggcgacgag gccggaggcg gcgccgaccg
    16321 aggtcggctt gccgtctttg atcttctcca cggcgagcca ggcgaggatc gaggcggccg
    16381 gcgcgacgag cgtgttgatg aatgccagga gcgcggtccc gtcgacggcg ccggcgctgc
    16441 cggcgttgaa cccgaaccag ccgatccaga ggaggccggc acccagcatg gtgagcggca
    16501 cgttatgcgg cttgtcgagc cccttgccga acccgttgcg tttgccgagc acgagggcca
    16561 aggcgagcgc ggcggcgccc gcgttgacgt gcaccgcgac cccgcccgcg aaatcgttca
    16621 cgcccatttg tgcggcccac ccgtcggccg ggtcgaagat ccagtgcgcg accgggaagt
    16681 acacgaggat cgaccagacc gccgcgaaga tgagccacga gccgaacttt gcccggtcgg
    16741 cgatcgcgcc ggagatgagg gcgacgacgt cgatggcgaa cgccgcctgg aaggccgcga
    16801 ccgcgacgag gtggatgtcg acggtgccgc cggggccgat gatgccgctc aggccgagat
    16861 cggtgaacgg gtcgccgatc caatgcggga tgaggggcga gccgaacgcg acaccgtaac
    16921 cgatcagcac ccagagcacg ccgacgacgg ccatcgcgcc gaaactcatc atcatcatgc
    16981 tgacgacgct cgtgacccgc accaggccgc cgtagaagaa cgcgacgccg ggggccatca
    17041 gcagggccag gatcgtcgcg accatcagcc acagcgtgtc cgcctgcgta tacgtcgccg
    17101 cctcggccgc gacatgaaat atcggcatcc ggtctccgtt cccgtcatca cgcggcggag
    17161 tcggccatgc ggcccgcatc gtgcgaataa ccgcagtctg gcgcaccgac gtttcacgac
    17221 ggtgtcgcaa acgtttcggt cttgttacac gtcgtagcgt gggctcatgc gggtgggagt
    17281 gatcggggcc ggggcgatcg gcggcacgat cgcggcgctt ctggcggatg ccggtcacga
    17341 agtctgggtg acggcgcgcg gcgcgcacgc cgccgcaatc aggaggacag gcctcgtcgt
    17401 cgagggcgcc tggggcacgc atcgccctcg ggtgcggatc gcccgtgaac tgccggccgg
    17461 ctgcgaggtc gtcttcgtct gcacgaaggc caacgacgcc cgccgggcgc tcgaaggctc
    17521 gcgtcgggcg ctcgccgggg cgtgcgtcgt gatcgtgcag aacggcctgc acggcaccgc
    17581 cgaggctgcg cggctgctgc ccggcacagg ggtggtgggg gccctggcgc tcttcgcggc
    17641 gacgatcgcg gagccgggcc gcatcgtcgt gacggccccg ggcgacctct gggtgggcgt
    17701 tgtcgcgggc gacccctccg cggcccagaa ggtcgcggcc cttctgggcc aggcgctgcc
    17761 cagccacgcg atcgacgacc tggccgcctg ccagtggacg aagctcatga tcaatcaggt
    17821 gaacgcgatg cccgccatca cggggctgag cgtgcaagag acgatcgccc gcccgcgcct
    17881 tcgccggatc atcgcccgga gcatgcggga ggcgacgcgg ctcgccttcg cgaccggcgt
    17941 gcgtttcgga cggctgcagg gcctcggccc ggggatccta cgtctcgtct cccggggccc
    18001 ccgcgccgcg gcgggcgcgc ttctcctggc gatggcccgg cggatgggcg acgtgccgaa
    18061 cctcggctcg acgttgcaga gcatccgccg tggcaggccg agcgaggtcg acgccctgaa
    18121 cggcgccgtg gtcgcctggg ccaaggagca cggccgggcc gcccccgtca acgaggcgct
    18181 ggcggcgatg gtgcacgagg tggaggcctc gggccgtttt ctgacggccg acgaggtcgc
    18241 ccggcgcttc acccgctgac cgtgctgccg ccggccgccc cccgtcagca gctgtcggcg
    18301 aagacgcgca gcggccggtt gtcgtcggtg ttgtcgatga tcgcgttcgc gacgccgcgc
    18361 gggaacacct cggcgaggta gcggtcttgc cccttgcggg tgcggcggtt cgccggatca
    18421 tccgggtcgg cggagggcgc ctggccggcc gcttggagac gcgcgtacgc cacagcccac
    18481 ggcacctcca gatagagcga gtagtgccag atgccgtgca gttcgggtcg gttcaggaaa
    18541 accccgtcga cgatgcagaa cgcgtcgtcg cccgccgtct gcggcgcggt ctcgaggctc
    18601 cgctcggccg cccggtcgta cgaggcggtg tggaacggcc ggccggcgag gctgaacggc
    18661 tcgacgagaa gccggcggaa ggccgcttcg tcgtattcgt cgtcgtagaa cccgtgcccc
    18721 gagtctgcgc cctgcctctc tcgcgcctcg cgcggagcgg cgaagtcggt gagggaggct
    18781 cgcacggtct cgtggccccg ctcccgcagc gcgtcggcga ggccgtcggc gaaggccgcg
    18841 gtgccggagc ccagaatgcc gtcgaccgcc agaaagatgc ggccgcgcga gtagttgtgc
    18901 gttatctcgt cggtgagcgc gtgccacagt tccacgcgcg gcgtcgtggt cagttccatg
    18961 cttcgagcct acgtcggggc ggccgaacac gtcgcgtggc acccgggccc gcttctatag
    19021 ccttgggggg tggatgacgc ggcccggacc cgattcgtgc gcagcctcac ccgctggtat
    19081 gcggggagcg cccgcgacct gccgtggcgt cggcccggct tcagcccgtg gggcgtgctc
    19141 gtgagcgaga tcatgctgca gcagacgccc gtcgcgcgcg tgattccccg gctcgaacag
    19201 tggctcacgc gctggccgac cccc
//
LOCUS       DASTNL010000058         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_598733, whole genome shotgun sequence.
ACCESSION   DASTNL010000058 DASTNL010000000
VERSION     DASTNL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598733"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFQ96_02035"
     CDS             complement(<1..915)
                     /locus_tag="VFQ96_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256602.1"
                     /translation="MSETLLYRPDHAVNLRETLAPLGRGPADPTTLWDEHGLWRSFRT
                     PQGPATLRLEDARPAVQVTAWGPGAPWAVAAAPQLLGSRDEPGMLERTPARGGGARGA
                     AGPDLIPRAADRRNDLAGPDAPGYHLVSESAHRHPGLRLMRTGLLLEALIPAILEQRV
                     TSTEAFRSWARLVRAYGEPAPGPRVDGAPRVPERLRVVPAPETWRRIPSWGWHKAGVD
                     PRRSRAVVEACRHAAALERLALLDGADARRRLTSIPGIGVWTAAEAVQRSHGDPDAVS
                     VGDYHLAAHVGTALIGRPVDDARMLRLLS"
     gene            1033..1872
                     /locus_tag="VFQ96_02040"
     CDS             1033..1872
                     /locus_tag="VFQ96_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEU0256603.1"
                     /translation="MARERIRQTMGFRRARVTGVTRPTPDFVRVTLAGADLADFRADG
                     PADHVKVYFPNPVTGVLTAPTWSEEHGVERPTDGLVISRDYTPRAWRPAAEGRPAELD
                     IDFFLYEGLGEHGNGPASSWAAGAAPGGEVAVGGPKGSLLAPAEARHALLVADETGLP
                     ALARWIDMLPAGCAITAVVLASDEATEAYLTAEEEARAGVEWLYRFDGPGQLEEAVHS
                     IDLADDAYVFAAGEAGELLPVRRMLKRDRHVDADHLQVQGYWKRGVTNRDHHAPIDPS
                     DPD"
     gene            1934..2377
                     /gene="panD"
                     /locus_tag="VFQ96_02045"
     CDS             1934..2377
                     /gene="panD"
                     /locus_tag="VFQ96_02045"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU0256604.1"
                     /translation="MLRSMLTGKIHRATVTHADLNYVGSITVDSTLLAAADIVEGEKV
                     TVVDITNGARLETYTIAGTADSGVIGMNGAAAHLIHPGDLVIIIAYGLVSDAEARTLR
                     PRIVHVDAANHIVSQGADAAAAPAGVPSGTAAPLSRPPLADEPAR"
     gene            2603..2944
                     /locus_tag="VFQ96_02050"
     CDS             2603..2944
                     /locus_tag="VFQ96_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256605.1"
                     /translation="MTEPQVWTLIGVFATATFGTITLISSLFVRVIRTEIGGLRGEVM
                     GEIGGLRGELTGEIGGLRGEVMGEIGGLRGEINGLRGEMNARFEAVDRRFDYLDRDVQ
                     ALTKHVFGTRD"
     gene            complement(3127..>3636)
                     /locus_tag="VFQ96_02055"
     CDS             complement(3127..>3636)
                     /locus_tag="VFQ96_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU0256606.1"
                     /translation="DEAAKIGGRIPVFDVNDPRFLPPGSRPGDPGGDMPTRIARWLRE
                     HDEQVPDSPAGFARSIIESLATAFARTMGEVGELTGERVRVIHLVGGGSQSALLCQRT
                     AELSGLPVVAGPAEATAIGNVLVQARALGAFRSSSPSASGSGAVASLEELRRLVARTT
                     RPIRYEPAA"
BASE COUNT          547 a         1305 c         1302 g          482 t
ORIGIN      
        1 ggagagcaag cgcagcatac gagcgtcgtc gacgggtcga ccgataagcg cggtgccgac
       61 gtgggcggcg aggtggtagt cgccgacgct caccgcgtcg gggtcaccgt gggaacgttg
      121 cacggcctct gcggcagtcc agacgccgat gcccgggatg ctcgtcagcc gccgacgggc
      181 gtcggcgccg tcgagaaggg ccaacctttc cagcgcggcg gcgtgcctgc atgcctcgac
      241 gacggcgcgg ctgcgacgcg ggtcgacacc cgccttgtgc cagccccacg acgggatacg
      301 ccgccaggtc tccggggccg gaacgacccg caaccgctcg ggcacgcgcg gcgcgccgtc
      361 gacacgcggc ccgggcgccg gctccccata ggcgcgtacg agccgcgccc acgagcggaa
      421 cgcctcggtg ctcgtcaccc gctgctccag gatggcgggg atgagcgcct cgagaagaag
      481 cccggtgcgc atcagccgca gaccgggatg ccgatgggcg ctctcgctca ccaagtgata
      541 tccgggggca tccggccccg caagatcgtt ccggcggtcg gccgcgcggg ggatgagatc
      601 gggcccagcc gcaccgcggg cacccccgcc ccgggcaggg gtgcgctcga gcatgccggg
      661 ttcgtcgcgg gagcccagca gctgcggcgc ggcggccacg gcccatgggg cacccggccc
      721 ccaggcagtg acttgcacgg cgggccgggc gtcttccaga cgtaaggtcg cggggccctg
      781 cggggtgcga aagctccgcc acagcccgtg ctcgtcccac agagtcgtcg ggtcggcagg
      841 gccccggccg agcggcgcga gggtctcccg cagattcacg gcgtgatccg gccggtagag
      901 aagagtctcg ctcacgcctc gccttcctcc ctcgtgtacg gtagcggcca ccgccgacac
      961 acgtcggcct gaacccccgc aaaccctcga aatggcggct tgaccgggcg aggcgcctgc
     1021 gagaataagc gaatggcacg cgaacgcatc cggcagacca tgggttttcg gcgcgcgaga
     1081 gtgacggggg tgacacggcc gacgcccgac ttcgtgcgcg tgacgctcgc cggcgccgac
     1141 ctcgccgact ttcgggccga cggcccggcc gatcacgtca aggtgtactt tccgaacccg
     1201 gtgacgggcg tcttgaccgc ccccacctgg agcgaggagc acggcgtcga acggccgacg
     1261 gacgggctcg tcatctcgcg cgactacacg ccgcgcgcgt ggcgcccggc ggcggagggg
     1321 cggccggcgg agcttgacat cgacttcttc ctctacgaag ggctcggcga gcacggcaac
     1381 ggcccggcgt cgtcgtgggc ggcgggggcc gcaccgggtg gcgaggtcgc ggtcggcggc
     1441 ccgaagggct cgctcctggc ccccgcggag gcgaggcacg cgctgctcgt cgccgacgag
     1501 acggggctgc ccgccttggc gcgctggatc gacatgctgc cggccggctg cgcgatcacc
     1561 gcggtcgtgc ttgccagtga cgaggcgacc gaggcgtacc tcacggcgga ggaagaggcg
     1621 cgggccggcg tggagtggtt gtaccgattc gacgggcccg gccagctgga agaggccgtg
     1681 cactcgatcg acctcgccga cgacgcctac gtcttcgcgg ccggcgaggc gggcgagctc
     1741 ttgccggttc gccgcatgct gaagcgcgac cgccacgtcg acgccgacca ccttcaggtg
     1801 cagggctact ggaagcgggg cgtcacgaac cgcgaccatc acgcgccgat cgacccgagc
     1861 gaccccgact gagcgcccgc cgtcagcctc agccacgggc ccgatctcgt cgggtggcgt
     1921 attctggagc gttgtgttga ggagcatgct gacgggcaag attcaccgtg ccacggtgac
     1981 gcatgccgac ctgaattacg tcggctcgat caccgtcgac agcacgttgc tcgccgcggc
     2041 cgacatcgtg gagggcgaga aggtcaccgt cgtcgacatc acgaacggcg cgcgcctcga
     2101 gacctacacg atcgccggca ctgccgacag cggcgtgatc ggcatgaacg gtgcggccgc
     2161 acacctcatc caccccggcg acctcgtcat catcatcgcc tacggcctcg tctccgatgc
     2221 ggaggcccgc acgttgcgcc cgcgcatcgt gcacgtcgac gcggcgaacc acatcgtgtc
     2281 gcagggcgcc gacgccgcgg ccgcaccggc cggcgtgccg tcgggcaccg cggccccgct
     2341 ctcccgccca ccgctcgccg acgagccggc ccgctagcat cccgcactcg cccgagacct
     2401 actcgtctct accgcccgcg acgctcccgc gtcggatcac ggcggcgagt gcggttcctc
     2461 cacagccgaa tccttcacaa cgcttctccc ccggtcggca gcggcgccga gccgggcatc
     2521 ccgctggcag ttagcgtcgc atcaggcgcc cgggaggctg gcgccgggtc gagcggttcg
     2581 ggagaataga agggtgttgc agatgacgga gcctcaggtg tggacgctga tcggcgtctt
     2641 cgccaccgcc accttcggca cgatcaccct catctcctct ctcttcgtgc gcgtcattcg
     2701 caccgagatc ggtgggctgc gcggcgaggt tatgggggag atcggcgggc tacgcggcga
     2761 actcacgggc gagatcggcg ggctccgggg cgaggttatg ggcgagatcg gcgggctccg
     2821 gggcgagata aacgggttac gcggggagat gaatgcccgg ttcgaagccg tcgaccgacg
     2881 cttcgactac cttgaccgcg acgtgcaagc cctgacgaag cacgtcttcg gcacccgcga
     2941 ctgagtcgag cctgccagcg aagcccatcg acggcccggc cccgcccggg ctgggttgct
     3001 cgtgcacccc gcggcacgcg taagcccagg accagcacgc ggcagcctca ccgcgcggtt
     3061 cctcaacgga cggcacgccc cgacatggcc gacctactcc gatcggtgcg gccgccacgt
     3121 cacggttcag gcggcaggtt cgtagcggat cgggcgagtg gtgcgggcga cgagacggcg
     3181 aagctcctcg aggctcgcca cggcccccga acctgacgcg ctcgggcttg acgagcgaaa
     3241 agcgccgagg gcgcgtgcct ggacgagcac gttgccaatg gcggtcgcct cggccgggcc
     3301 ggcgacgacg ggcaggcccg agagctctgc cgtgcgctgg cagagcagcg cgctctgaga
     3361 accgccaccc acgaggtgga tcacccgcac ccgctcgccc gtgagctcac caacctcgcc
     3421 catcgtgcgc gcgaacgccg tggcgagaga ttcgatgatg cttcgcgcga agccggccgg
     3481 cgagtcgggc acctgctcgt catgttcgcg cagccaccgg gcgatgcggg tgggcatgtc
     3541 gccgcccggg tcaccgggcc gggagcctgg gggcaagaag cgcgggtcgt tcacatcaaa
     3601 gacgggtatc cggcccccga tcttggccgc ctcgtc
//
LOCUS       DASTNL010000059         8382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_417704, whole genome shotgun sequence.
ACCESSION   DASTNL010000059 DASTNL010000000
VERSION     DASTNL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417704"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..552
                     /locus_tag="VFQ96_02060"
     CDS             79..552
                     /locus_tag="VFQ96_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256607.1"
                     /translation="MADNRNGQGRLSATERAAVKQRSAELRNEARAGKAAERRERDAQ
                     ACAAAIEALTGADRTVAERLNAIVTAEAPELLPKTWYGFPSYARDGKVVVFYQPASKF
                     GTRYGTVGFSEDARLDDGAAWPVAYAVLAVTEEVEQDLRTLVRRAAGASAGQPGA"
     gene            complement(596..3208)
                     /gene="valS"
                     /locus_tag="VFQ96_02065"
     CDS             complement(596..3208)
                     /gene="valS"
                     /locus_tag="VFQ96_02065"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU0256608.1"
                     /translation="MTDLDRVPDKPALEGLEATWSARWEEQGTYRFSRDQLRHDAIYS
                     IDTPPPTASGSLHIGHVFSYTHTDIIARYHRMRGKNVFYPMGWDDNGLPTERRVQNYF
                     GVRCDPSLAYEPGFVPPHQGDGASVKAADQQPVSRRNFIELCQRLTIEDERQFESLWR
                     ALGLSVDWSQTYRTIGEKSLRASQLAFLRNVKRGEAYQAMAPTLWDVTFRTAVAQAEL
                     EDRDRPGAYHRVAFHGDDGPVYIETTRPELLPSCVALVAHPDDERYRHLFGTTVTTPL
                     FGVRVPVLPHRLAEPDKGSGIAMICTFGDVTDVVWWRELKLPNRSVLGFDGRLSAETP
                     DWLSLAEARSAYGRLAGKTVFSARREVVDMLRESGLLDGEPKPVTHPVKFFEKGDRPL
                     EIVSTRQWYVRNGATDEALRAGLMAAGRQLHWHPDFMRVRFENWVAGLAGDWLISRQR
                     FFGVPIPVWYPVDDAGDPVYDTPILPDEARLPVDPSSEPAPGFSEKQRGVPGGFVGEV
                     DVMDTWMTSSLTPQLAAGWERDPELFQAVFPYDLRPQGQDIIRTWLFSTLLRSRLEHG
                     VLPWSDTTLSGFIVDPDRKKMSKSKGNVVTPAAILEKHGSDAVRYWAASSRLGTDAAF
                     DVNNPTQIKIGRRLAIKVLNAAKFILGFDATDEAASEPLDLALLAALATVVDEATRAL
                     EEYDHAKALETIESFFWTFCDDYLELVKERAYGSGEQGEAGRRSAVATLRRALFVMLR
                     LFAPFLPFVAEEAWSWSHDTSVHRSPWPDRTGLLPNAGKAGQGHHRAVTESDTAALTA
                     ASAALVGIRRAKTDAKVSQKTAVVSCRLTAPADRLADLQAVRADLKAVGKIASLDVVA
                     GDAFAVTDVVLAER"
     gene            3326..3976
                     /locus_tag="VFQ96_02070"
     CDS             3326..3976
                     /locus_tag="VFQ96_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256609.1"
                     /translation="MTDQQRHRGRRPASSRATLVDAADELFLEQGYAATTIEQITRRA
                     GVSRNTFFNYFAAKGDLLFAGVDDICAALAARLGEAGAAGSLAALRDAIVRTAAEVDV
                     RRIPLAATQWGAMGILGEARAAGLARFATLAETMRRFLERGAADPGGEVGAAAGAGPQ
                     GKALWAQAAAYALTAASVAAGLRWLASGVGRGPLAGFIVAAVDPVCAGFSWPPASL"
     gene            4314..7640
                     /gene="ileS"
                     /locus_tag="VFQ96_02075"
     CDS             4314..7640
                     /gene="ileS"
                     /locus_tag="VFQ96_02075"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU0256610.1"
                     /translation="MPHGGAGAAFGLDPSPVFPRLEEDVLDFWRRDDTFAASVQARQG
                     CPEWVFYDGPPFANGLPHYGHLLTGYAKDLFPRYQTMRGMLVHRRFGWDTHGLPAELE
                     AERQLGITDKSQIEKMGIAQFNEAARQSVLKYTREWQEYVTRQARWVDFEGGYKTLDT
                     DYMESVLWAFKQLYDKGLAYEGYRVLPYCWRDQTPLSNHELRMDDDVYKMRQDQTVTI
                     TFPLEGEKARAAGLEGVKAIAWTTTPWTLPTNLALVVGPDIVYAVVPAGPEGAADTAD
                     GGAGSYLLARDLVGAYAKDLGYHDEAAAVAAVTATIPGRELADIRYRRLWDYYADAER
                     FGTGGAWRILVDDYVATDEGTGIVHQAPAYGEDDQRVCAAAGIPVVVSVDAGGRFLPD
                     VPEVAGLQVFDANMPIVRMLRLAGRLLRLASYEHSYPHCWRCRNPLIYKAVSSWFIRV
                     PEFRDRMVQINQQIEWVPENVKDGQFGKWLAGARDWSISRNRYWGSPIPVWKSDDPQY
                     PRIDVYGSLAELERDFGALPHNDDGEVDLHRPYVDALTRPNPDDPTGGSTMRRITDVL
                     DVWFDSGSMPYAQVHYPFENREWFDTHNPADFIVEYIGQTRGWFYLLHVLATALFDRP
                     AFKRVVSHGIVLGNDGQKMSKSLRNYPDVNEVFDRDGSDAMRWFLMSSPVLRGGNLIV
                     TEEGIREGVRQVILPLWNTWYFFALYANACGYEARHDVDSTDVLDRYLLAKTHDLIVT
                     VTANLDALDATSAAAELRDFADILTNWYVRRSRDRFWAGVREADGRRTGTEAFDTLYT
                     VLEAFARLAAPLLPMVTERVWKGLTGGRSVHLTDWPAAAAFPEDGALVRAMDRVRAVT
                     STALSLRKRAGLRVRLPLSRLSVIAPEASSLAPFSDILHEELNVKAVDFDELAPDSAA
                     RYGISTRLSVNARAAGPRLGRDVQKAIAAARAGEWSAEGGEVVAGGIRLIEGEYEIVL
                     DAGSASGGTALLPGGGFVLLATDTTPELEAEGLARDVIRAVQDTRKAAGLDVSDRIHL
                     VVAFASEDDREAVSLAHEAVDLAGETLATLLQIVSLGEPGETGAAAAEQGEAFGQEFA
                     AGRFANAGVFRVSVAKEPVR"
     gene            7637..>8382
                     /locus_tag="VFQ96_02080"
     CDS             7637..>8382
                     /locus_tag="VFQ96_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU0256611.1"
                     /translation="MSATGPARGEASAEADAIYAELLLRLGEGAPERRLHATRKAVEL
                     LGDPHRAYPIIHVTGTNGKTSTSRMIEALLRAHNLTTGLFTSPHLARFTERIVIGGRP
                     ISDADLVRNWNDVAPYIAMTDRELHEAGEQRLTFFEAMTVLGFACFADAAVDVAVIEV
                     GMGGEWDSTNVGDGQVAVITPISLDHMAMLGNTVAEIAHTKSGIIKPGSRVVSSAQPE
                     PALQEIRLAARRLDVPLAVQGEDFDVTADA"
BASE COUNT         1314 a         2837 c         2956 g         1275 t
ORIGIN      
        1 caggagaaat ccgcctgagt gtgggccgcc ggagtcggtc cggggcgctt gggccaagcc
       61 tggacgaggg ggaacatcgt ggcagacaac aggaatgggc agggccggct gagcgccacc
      121 gagcgcgccg cggtgaagca gcgttccgct gaattgcgaa acgaggcgcg ggcgggtaaa
      181 gcggccgagc ggcgagagag ggacgcgcag gcctgcgccg ccgcgatcga agctctgacc
      241 ggcgcggatc gcaccgtggc cgagcggctg aacgccatcg tcactgcaga ggcgccagaa
      301 ctattgccga agacgtggta cgggttcccc tcctatgccc gtgacggcaa agtcgtggtc
      361 ttctaccagc cggcctccaa gttcggcaca cggtacggca cagtcgggtt cagtgaagac
      421 gcgcgtctcg acgacggcgc cgcctggccc gtcgcctacg ccgtcctggc ggtgacggag
      481 gaggtcgaac aggacctgcg cacccttgtt cgccgggccg ccggagcgag cgcagggcag
      541 ccgggcgcct gacgggcact gtgccacccg cggccacccc cgcgtgcccg gcggctcagc
      601 gctcggccag caccacgtcg gtgacggcga aagcgtcgcc tgccaccacg tcgagggagg
      661 cgatcttgcc gacagccttg aggtcggcgc gcaccgcctg aaggtctgcg aggcggtcgg
      721 cgggcgccgt caaccggcag gagacgaccg ccgtcttctg tgacaccttg gcgtcggtct
      781 tcgcccgacg gatgccgacc agggcggcac tggctgcggt gagtgcggcg gtgtcggatt
      841 ctgtcaccgc ccggtggtgg ccttggccgg ccttgcccgc gttcggcaga agtcccgtgc
      901 ggtcgggcca aggggagcga tgcaccgagg tgtcgtgcga ccacgaccag gcctcctcgg
      961 ccacgaacgg caggaatggc gcgaagaggc gcagcatgac gaagagtgcc cgccgcagcg
     1021 tggcgacggc ggagcggcgc cccgcctcgc cttgctcgcc gctgccgtag gcgcgttcct
     1081 tgacgagctc gaggtagtcg tcgcagaagg tccagaagaa cgactcgatg gtctccaacg
     1141 ccttggcgtg gtcgtattcc tcgagcgccc gggtcgcctc gtcgacgacc gtcgccaggg
     1201 ctgccagaag ggcgaggtcg aggggttcgc tcgccgcctc gtcggtcgcg tcgaagccta
     1261 ggatgaactt cgccgcgttc agcaccttga tcgcgagccg gcggccgatc ttgatctgcg
     1321 tcgggttgtt cacgtcgaat gcggcgtcgg tgccgagcct cgacgacgcg gcccagtagc
     1381 gcaccgcgtc ggagccgtgc ttctcgagga tcgccgcggg cgtcacgacg ttgcccttgg
     1441 acttcgacat cttcttgcgg tcggggtcga cgatgaagcc gctcagcgtg gtgtcgctcc
     1501 acggcagcac gccgtgctcc agacgggagc gaagcagcgt cgagaagagc cacgtgcgga
     1561 tgatgtcttg cccctgtggc cgtaggtcgt agggaaagac ggcctggaag agttcgggat
     1621 cccgttccca gccggccgcg agttgtgggg tcaacgaaga ggtcatccac gtgtccatca
     1681 cgtcgacctc gccgacgaag ccgcccggca cgccacgctg tttctcggag aagcccggtg
     1741 ccggctccga cgacgggtcg accggcagcc gcgcctcgtc gggcaagatc ggcgtgtcgt
     1801 agaccgggtc gccggcgtcg tcgaccgggt accagaccgg gatcggcacg ccgaagaaac
     1861 gttgacgcga gatgagccag tcgccggcga gccccgcgac ccagttctcg aaccgcacgc
     1921 gcatgaagtc ggggtgccag tgcaactgac gccccgcagc catgagcccc gcccgcaagg
     1981 cctcgtcggt cgcgccgtta cgcacgtacc actgacgggt cgagacgatc tcgagcggcc
     2041 ggtcgccctt ctcgaagaac ttcacggggt gcgtgacggg cttcggctcg ccgtcgagga
     2101 ggcccgactc gcgcagcatg tcgaccacct cgcgccgggc gctgaagacc gtcttgcccg
     2161 cgagccgtcc gtacgccgag cgtgcctccg ccaggctcag ccagtcgggg gtctcggcgc
     2221 taagacgccc gtcgaagccc aacacggaac ggttcggcag cttcagctcg cgccaccaga
     2281 cgacgtcggt cacgtcaccg aaggtacaga tcatcgcgat gccgctgccc ttgtcgggct
     2341 cggcgagccg gtggggcaga accggcaccc gcaccccgaa gagaggcgtc gtcaccgtgg
     2401 tgccgaaaag gtgccggtag cgctcgtcgt ctggatgggc gacgagagcg acgcacgagg
     2461 gcaggagctc cggcctcgtc gtctcgatgt agaccgggcc gtcgtcgccg tgaaaggcca
     2521 cgcgatggta ggcgcctggc cggtcgcggt cttccagctc ggcctgcgcg acggccgtgc
     2581 ggaacgtcac atcccacagg gttggggcca tcgcctggta tgcctcgccg cgcttgacgt
     2641 tgcgcaggaa cgccagttgg gaggcacgca acgacttctc cccgatggtg cggtaggtct
     2701 ggctccaatc cacggagagg ccgagcgcgc gccagaggct ctcgaactgc cgctcgtcct
     2761 cgatcgtcag gcgctggcac agctcgatga agttccggcg tgagacgggc tgctggtcgg
     2821 ccgccttgac gctcgcgccg tcgccctggt ggggcggcac gaagccgggc tcgtaggcga
     2881 ggctcgggtc gcagcgcacg ccgaagtagt tctgcacccg gcgttccgtg ggcaggccgt
     2941 tgtcatccca gcccatggga tagaagacgt tcttgccgcg catccggtgg tatcgggcga
     3001 tgatgtcggt atgcgtgtaa ctgaacacgt ggccgatgtg cagggacccc gacgcggtcg
     3061 gcgggggcgt gtcgatcgaa tagatggcgt cgtggcggag ctggtcgcgt gagaagcggt
     3121 aggtgccctg ctcctcccac cttgccgacc aggtggcctc cagaccctcg agcgcgggct
     3181 tgtcgggaac acggtcgagg tcggtcatgg cgtcgctttc ggctcgttat gggcggcatc
     3241 gtgtctctgg atgcctggat gtggggtgta accagcctaa acgatgaggg tgacgcgccc
     3301 ggcgccgacg ggcgagaatg atcgggtgac agaccagcaa cgacaccggg gccgcaggcc
     3361 cgcgtcgtct cgggcgacgc tggtcgacgc ggccgacgag ctgtttctgg aacagggcta
     3421 cgccgccacc acgatcgagc agatcacccg ccgggccggc gtcagccgca atacgttctt
     3481 caactatttc gcagccaagg gcgacttgct cttcgccggc gtcgacgaca tctgtgccgc
     3541 cttggcggcc cgcctcggcg aggccggcgc ggcgggatcg ctggcggcat tgcgcgatgc
     3601 gatcgttcga accgcggccg aggtggacgt gcggcggatc ccgctggcgg cgacccagtg
     3661 gggcgcgatg gggattctgg gtgaggctcg cgcggcgggt ctcgcccgct ttgcgacgct
     3721 cgctgagacg atgcggcgtt tcctggagcg gggggcagcc gaccccgggg gcgaggtcgg
     3781 cgccgcagcg ggggccgggc cgcaggggaa ggcgctgtgg gctcaggcgg ccgcgtacgc
     3841 gctcaccgcc gcgagtgtcg cggcgggatt gcgctggctc gcctccgggg ttgggcgcgg
     3901 gccgttggcc ggcttcatcg tggccgcggt cgacccggtg tgtgcgggtt tctcttggcc
     3961 gccggcgtct ctgtgaacgt cccgcagccg atagtatgag ggcacacgcg acaatccggc
     4021 gatcaccggg gagcggtcgg aagagcggcc ttgcggccgg gtagaaccga acgggtgggc
     4081 ccgtcatagc ccgagagcgg ttgcgaaggc ggcaccgggc ccgcaaggtt ccggaagtcg
     4141 cgggcagcaa gcgaggtggt accgcgacac ccggagccgg gcggcgtcct cgcagaagga
     4201 tgaaccctga cgttgccggg agaataatgc cgtatccgcg atccgacgac cgccgcgagc
     4261 cgagcctcca gagccccggg ccgggggctc ctgccgcgca gcccgacggg ccgatgccgc
     4321 atgggggtgc cggcgccgcc ttcgggctcg acccgagccc cgtgtttccg aggctcgaag
     4381 aggacgtgct cgacttttgg cgccgggacg acacgttcgc cgcgtcggtc caagcccggc
     4441 agggctgccc cgaatgggtc ttctacgacg gcccgccgtt cgccaacggc ctgccgcact
     4501 acggccacct cctcaccggg tacgccaaag acctctttcc gcgctaccag acgatgcgcg
     4561 gcatgctcgt gcaccgccgg ttcggctggg acacccacgg tctgcccgcc gagttggagg
     4621 cggaacgcca gctcggcatc accgacaaga gccagatcga gaagatgggc atcgcccagt
     4681 tcaacgaggc ggccaggcag tcggttctga agtacacccg cgaatggcag gaatacgtca
     4741 cccgccaggc ccgctgggtc gacttcgagg gtggctacaa gaccctcgac accgactaca
     4801 tggagagtgt cctgtgggcg ttcaagcagc tctacgacaa gggcctcgcc tacgagggct
     4861 accgggtgct gccctactgc tggcgcgacc agacgccgct gtcgaaccac gagctgcgga
     4921 tggacgacga cgtctacaag atgcggcaag accagaccgt gacgatcacc ttccccctcg
     4981 agggggagaa ggcgcgtgcg gcgggcctcg aaggggtcaa ggcgatcgcc tggacgacca
     5041 cgccgtggac cttgccgacg aacctcgcgc ttgtcgtcgg ccccgatatc gtttacgctg
     5101 tcgtgccggc cggcccggag ggcgccgcgg acacggccga tgggggggca ggcagctacc
     5161 ttctcgcccg cgaccttgtg ggcgcctacg cgaaagacct cggctatcac gatgaggccg
     5221 cggctgtcgc ggcagtcacg gcgacgattc cgggccgcga gctcgccgac atccgctacc
     5281 gccggctctg ggactattac gccgacgccg aacggttcgg cacgggcggc gcctggcgca
     5341 tcctcgtcga cgactatgtc gccaccgacg aggggacggg catcgtgcat caggcgcccg
     5401 cgtatggcga agacgatcag cgggtgtgtg ccgccgccgg catcccggtc gtcgtctccg
     5461 tcgacgccgg cggacgtttc ctgcccgacg tgccggaggt ggcgggcctg caggtcttcg
     5521 acgcgaacat gccgatcgtg cgcatgctgc gccttgccgg ccggctcctc aggctggcca
     5581 gctacgagca ctcgtacccg cactgctggc ggtgccgcaa cccgctgatc tacaaggcgg
     5641 tctccagctg gttcattcgg gtgccggagt tccgcgaccg catggtgcag atcaaccagc
     5701 agatcgagtg ggtaccggag aacgtcaaag acggccagtt cggcaaatgg ctcgccggcg
     5761 cccgcgactg gtccatcagc cgcaaccggt attggggctc gcccattccg gtgtggaaga
     5821 gcgacgatcc ccagtacccg cgtatcgacg tgtacgggtc gctggcggag ttggaacgcg
     5881 acttcggcgc gctgccgcac aacgatgacg gcgaggtcga cctgcatcgc ccgtatgtcg
     5941 acgccctgac gaggccgaac cccgacgacc cgaccggcgg ctccaccatg cgtcgcatca
     6001 ccgacgtgct cgacgtctgg ttcgattcgg gctctatgcc gtacgcgcag gtgcattacc
     6061 cgttcgagaa ccgggaatgg ttcgacaccc acaacccggc cgacttcatc gtcgagtaca
     6121 tcggccagac ccgcggctgg ttctacctgc tgcacgtgct cgccaccgcg ctcttcgacc
     6181 ggcccgcatt caaacgcgtc gtcagccacg gcatcgtgct cggcaacgac ggccagaaga
     6241 tgtccaagtc gctgcgcaat taccctgacg tcaacgaggt tttcgatcgc gacggttccg
     6301 acgcgatgcg ctggttcctg atgtcgagcc ccgtgctgcg cggcggcaac ctcatcgtca
     6361 ccgaggaggg catccgggag ggcgtgcgtc aggtgatcct gccgctctgg aacacgtggt
     6421 acttcttcgc cctctacgcg aacgcgtgcg gctacgaggc gcggcacgac gtcgactcga
     6481 cggacgtgct cgaccggtat ctgttggcga aaacccacga cctcatcgtc acggtcaccg
     6541 cgaatctcga cgcgttggat gcgacgagcg cggccgcgga gctgcgcgac tttgccgaca
     6601 ttctcaccaa ctggtacgtg cggcgttccc gcgaccggtt ctgggcgggc gttcgtgagg
     6661 ccgacggacg ccgcaccggc accgaggcgt tcgacacgct gtacaccgtg ctcgaggcgt
     6721 ttgcgaggct cgccgcgccg ctcctgccga tggtcaccga gcgggtctgg aagggcctga
     6781 cgggcgggcg cagcgtgcac ctgaccgact ggcccgcggc ggcggccttc cccgaagacg
     6841 gggcgctggt ccgggcgatg gaccgcgtgc gcgccgtcac ctccaccgct ctctcgttgc
     6901 gcaagcgagc cgggctgcgg gtgcgcctgc cgctttcccg gctttccgtc atcgcccccg
     6961 aggcatccag cctcgcgccg ttctccgaca ttctgcacga ggagctcaac gtcaaagcgg
     7021 tcgacttcga cgagctggca cccgacagcg cggcacggta cggcatcagc acgcggttga
     7081 gcgtgaacgc gagggcggcg ggcccccggc tggggcgcga cgtgcagaaa gcgatcgccg
     7141 ccgcgcgggc cggcgagtgg agtgccgagg gcggcgaggt cgtcgccggt ggcatccggc
     7201 tcatcgaggg cgaatacgag atcgtgctcg acgccggctc cgcctccggc ggcaccgccc
     7261 tcttgccggg cggcggcttc gtgctgctcg ccaccgacac cacgccggag ctggaggcgg
     7321 aaggcctggc ccgcgacgtc atccgtgccg tgcaagacac gcgcaaggcg gcggggctcg
     7381 acgtgagcga ccggatccac cttgtcgtcg ccttcgcctc ggaagatgat cgggaggccg
     7441 tcagtctcgc ccacgaggcc gtcgacctgg ccggcgagac gctggcgacc ctgctgcaga
     7501 tcgtctctct cggcgagccc ggcgaaaccg gggccgcagc agcagagcag ggtgaagcct
     7561 tcgggcagga gttcgccgcc ggccggtttg cgaacgccgg cgttttccgc gtcagcgtag
     7621 cgaaggagcc cgtccgatga gcgcgacggg tcccgcccgg ggcgaggcgt cggccgaggc
     7681 agacgccatc tatgccgagt tgctgttgcg cctgggggag ggcgcccccg agcggcggct
     7741 gcacgccacg cgaaaggccg tcgaactctt gggcgaccca caccgcgcct acccgatcat
     7801 ccacgtgacg ggcaccaacg gcaagacgtc gacgagtcgg atgatcgagg cgctcctgcg
     7861 cgcccacaac ctgacgaccg ggctcttcac gagcccgcac ctggcacgct tcaccgaacg
     7921 catcgtcatc ggcgggcggc cgatctccga cgccgacctg gtgcgtaact ggaacgacgt
     7981 tgcgccgtac atcgccatga ccgaccggga gcttcacgag gccggcgagc aacgcctcac
     8041 cttcttcgag gcgatgaccg tgctcggctt cgcctgcttc gccgacgcgg ccgtcgatgt
     8101 cgcggtcatc gaggtcggga tgggcggcga atgggattcc accaacgtcg gagacggcca
     8161 ggtggcggtc atcaccccga tctcactcga tcacatggcg atgctcggca acaccgtcgc
     8221 cgagatcgcg cacaccaaat ccggcatcat caagcccggt tcccgcgtcg tcagctccgc
     8281 ccagccggag ccggccctcc aagagatccg cctggccgcg cgccgtctcg acgtacccct
     8341 cgcggtgcaa ggtgaggatt tcgacgtcac ggccgacgcg at
//
LOCUS       DASTNL010000060         4599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_232170, whole genome shotgun sequence.
ACCESSION   DASTNL010000060 DASTNL010000000
VERSION     DASTNL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232170"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..899)
                     /gene="rplQ"
                     /locus_tag="VFQ96_02085"
     CDS             complement(297..899)
                     /gene="rplQ"
                     /locus_tag="VFQ96_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0256612.1"
                     /translation="MPQPTKGPRLGGSPAHERLMLANLATSLFQYKSIKTTHTKAKRL
                     RPVAERLITAAKRGDLHARRRVAAVIRDKSVVHELFTQIAPLVADRPGGYTRITKLGF
                     RKGDNAPMSQIELILDPVAPKHRKSADRPAARDEARARGAAAATESEAEEAPEAAAAA
                     SGDAAGVEPTASAEGSAVEDGPSAAADVADDAATKKDEAE"
     gene            complement(945..1937)
                     /locus_tag="VFQ96_02090"
     CDS             complement(945..1937)
                     /locus_tag="VFQ96_02090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU0256613.1"
                     /translation="MLIAQRPTLVEENVSEYRSRFVIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFSTVPGVKEDVTEVILNIKGLVVSSEHDEPITAYLRKTGA
                     GQVTAADISAPAGVEVHNPELVIATLNENAKLEIELTIERGRGYVSAVQNRNEYAEAG
                     QIPIDSIYSPVLKVTYRVDATRAGERTDFDRLVVDVETKPAISPRDAVASAGRTLVEL
                     FGLARDLNEDAEGIEIGPAPVEQMLDSELLVPIEDLDLSVRSYNCLKREGINNVSELV
                     SLSENQLMNIRNFGQKSVDEVKEKLTQMGLSLKDAVPGFDGAHFYSGYDEEEIN"
     gene            complement(2027..2425)
                     /gene="rpsK"
                     /locus_tag="VFQ96_02095"
     CDS             complement(2027..2425)
                     /gene="rpsK"
                     /locus_tag="VFQ96_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU0256614.1"
                     /translation="MAAPKTAVRKPRRKDRKNIAVGQAHIKSTFNNTIVTITDTTGGV
                     ISWASSGGVGFRGSRKSTPYAAQMAAESAARKAQEHGMKKVDVFVKGPGSGRETAIRS
                     LQAAGLEVGSINDVTPQAHNGCRPPKRRRV"
     gene            complement(2459..2833)
                     /gene="rpsM"
                     /locus_tag="VFQ96_02100"
     CDS             complement(2459..2833)
                     /gene="rpsM"
                     /locus_tag="VFQ96_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU0256615.1"
                     /translation="MARLAGVDIPREKRVEVALTYIYGVGRTRALKTLAETGIDGDTR
                     VRDLSDDQMLALRDYIEGNFKVEGDLRREVAADIRRKVEIGSYEGIRHRRGLPVRGQR
                     TKTNARTRKGPKRTVAGKKKAR"
     gene            complement(2973..3086)
                     /gene="rpmJ"
                     /locus_tag="VFQ96_02105"
     CDS             complement(2973..3086)
                     /gene="rpmJ"
                     /locus_tag="VFQ96_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU0256616.1"
                     /translation="MKVKPSVKKICDKCKIVRRHGRVMVICENPRHKQRQG"
     gene            complement(3156..3377)
                     /gene="infA"
                     /locus_tag="VFQ96_02110"
     CDS             complement(3156..3377)
                     /gene="infA"
                     /locus_tag="VFQ96_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0256617.1"
                     /translation="MAKKDGVIEIEGHVTEALPNAMFRVELTNGHKVLARISGKMRQH
                     YIRILPQDRVVVELSPYDLTRGRIVYRYR"
     gene            complement(3539..4372)
                     /gene="map"
                     /locus_tag="VFQ96_02115"
     CDS             complement(3539..4372)
                     /gene="map"
                     /locus_tag="VFQ96_02115"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU0256618.1"
                     /translation="MRGMRHTIYKTPAQLRLMIPPGLATAASLDACEAALRPGITTGE
                     LDRLAERAIRDLGAEPNFVLEPGYHNTVCASVNDEVVHGIPGTRVVRAGDLVSIDSGA
                     IQNGWNGDAARSFVVPDEDRPDVVAARARLNEVTRRSLWHGIAALAAASYLYEVGAAI
                     EGFVRSQGDYGVLTDYVGHGIGRHMHEDPPVPNYRVKQKGPEVRPGLVVAIEPMITQG
                     SPATSVRPDGWTVVTDDHGDAAHWEHTVAVHKDGIWVLTAADGGARELAEFGIVPVRP
                     A"
     gene            complement(4373..>4599)
                     /locus_tag="VFQ96_02120"
     CDS             complement(4373..>4599)
                     /locus_tag="VFQ96_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU0256619.1"
                     /translation="VRRLRHRAEEEGRSDDTDEGIRHRQAVYREQTAPILGLFRDRGL
                     LAEVDGLGSPDEVLGRVLDALLQNGVGANA"
BASE COUNT          641 a         1526 c         1569 g          863 t
ORIGIN      
        1 gtcgggcggc gaagtttatg tcaccgcgct acgggccgcg ttgcaacagc ttgtcgatga
       61 gccctgccga gactgtttcc tgcggggcgg tcccgccgag cgactccttc gcggtgactg
      121 aggcgcgaaa ccgcacgctc cggccgagga gtccgctcga gagggcgtcg tagcacgacg
      181 ccgatccgca cgcctcggcc cgcggcgagg gccgggaaac acgaagggcc cccgtcgtgg
      241 gggcccttcg cagggttcac tcgcaggccg gctgcgcggc ccgggcgaag aaggacctac
      301 tcggcctcgt ccttcttcgt cgccgcgtcg tcggcgacgt ctgccgcggc cgaaggcccg
      361 tcctcgaccg cggacccctc ggcagaagcc gtcggctcca cgcctgcggc atcccccgag
      421 gctgccgccg cggcctcggg ggcctcctcg gcctcggatt ctgtcgccgc ggcagcgccg
      481 cgcgcacgcg cctcgtctct cgcggccggt cgatcggcac tcttgcggtg cttgggcgcc
      541 accgggtcga ggatcaactc gatctgcgac atcggggcgt tgtccccctt gcggaagccg
      601 agcttcgtga tgcgggtgta gccgcccggc cggtccgcga cgagcggcgc gatctgcgtg
      661 aagagctcgt gcacgacgct cttgtcgcgg atgaccgcgg cgacgcggcg acgggcgtgc
      721 aggtcgcccc gtttggctgc cgtgatgagc cgttcggcga ccgggcgcag gcgcttcgcc
      781 ttcgtgtggg tcgtcttgat cgacttgtat tggaaaagcg acgtcgccag gttggcgagc
      841 atcagccgct cgtgcgccgg gctgcccccg agacgagggc ccttggtcgg ttgaggcatt
      901 gctattctcc cgtgctgaaa gggtggtcag gtcggcagcg ccggctagtt gatctcttct
      961 tcgtcgtagc cgctgtagaa gtgcgcgccg tcgaaaccgg gcaccgcgtc cttcaacgac
     1021 aggcccatct gcgtcagctt ctccttgacc tcgtccaccg acttctggcc gaagttgcga
     1081 atgttcatca actggttctc ggagagcgag acgagttcgc tgacgttgtt gatgccctcg
     1141 cgcttgaggc agttgtacga gcgcaccgag aggtcgaggt cttcgatcgg cacgagtagt
     1201 tcggagtcga gcatctgctc gaccggggcg gggccgatct cgatgccttc cgcgtcttcg
     1261 ttcaggtcgc gggccaggcc gaacagctcg acgagcgtgc ggccggcgga ggcgacggcg
     1321 tcgcgcgggc tgatggcggg cttggtctcg acgtcgacga cgagacggtc gaagtcggtg
     1381 cgctcgccgg cccgcgtcgc gtccacccgg taggtcacct tcaagacggg cgagtagatg
     1441 gagtcgatcg ggatctgccc cgcctccgcg tactcgttcc ggttctggac ggccgagaca
     1501 tagccgcggc cacgctcgat cgtcagctcg atctccagct tggcgttctc gttcaaagtg
     1561 gcgatgacga gctccgggtt gtgcacctcg acccctgccg gcgcggagat gtccgccgcc
     1621 gtgacctggc cggcgccggt cttgcgcagg taggccgtga tcggttcgtc atgctcgctg
     1681 gagacgacga ggcccttgat gttcaggatg acctcggtca cgtcttcttt cacacccggg
     1741 acggtgctga actcgtgcag caccccgtcg atgcggatgc tcgtgacggc cgcacccgga
     1801 atcgacgaca ggagggtgcg tcgaagagag ttcccgaggg tgtagccgaa gcccggctcc
     1861 aacggttcga tgacgaaccg cgaacggtac tccgacacgt tctcttcgac gagcgtggga
     1921 cgctgtgcaa tgagcactgt tgattccttt cggccaagtg tccgctatat gacacctgcg
     1981 tatgggagaa gtggtgttga gttatgccga cggtcttcgg ggcccgtcag acccggcgcc
     2041 tcttcggcgg ccggcaaccg ttgtgcgctt gcggcgtcac atcgttgatc gaaccgacct
     2101 cgagccccgc ggcctgcagg gagcggatcg ccgtctcgcg acccgagccc gggcccttca
     2161 cgaagacgtc caccttcttc atgccgtgct cctgcgcctt gcgggcggca gactcggccg
     2221 ccatctgtgc cgcatagggg gtcgacttgc gactgccgcg gaagccgacg ccaccggaag
     2281 aggcccagct gatgacgccg ccggtcgtgt cggtgatcgt gacgatggtg ttgttgaacg
     2341 tcgacttgat gtgggcctgg cccacagcga tgttcttgcg gtcttttctg cgcggcttgc
     2401 gaacggccgt cttgggtgct gccatgagtt ctcctggagt ccttgtccta tgcccggcct
     2461 agcgtgcctt cttcttaccg gcgaccgtgc gcttcgggcc cttgcgggtg cgcgcgttcg
     2521 tcttggtgcg ctgaccgcgc accggcaggc cgcggcgatg gcggatgccc tcgtagctgc
     2581 cgatctcgac cttgcgtcga atgtctgcgg cgacctcgcg acgaaggtct ccctcaacct
     2641 tgaagttgcc ctcgatgtag tcgcgcagcg cgagcatctg gtcgtcgctc aggtctcgca
     2701 cccgggtgtc gccgtcgatg ccggtctcgg ccagcgtttt cagggctcgg gtgcgtccca
     2761 ctccatagat ataggtcaat gcgacctcca cgcgcttctc gcgcgggatg tcgactcctg
     2821 ccagacgtgc catgacgcct tctcctgtcg gtgagtggag gtgttcagca ttcccggtgc
     2881 cgcggcctcc gaccgcgggt gtcgcccgtc acgggttcgt gggatgcggg gttcattgtg
     2941 gtgccgcctg ggcgagagcc tcgttgcggc gctcagccct ggcgctgctt gtgcctcggg
     3001 ttctcgcaga tgaccatcac gcgaccgtgc cggcgcacga tcttgcactt gtcgcagatc
     3061 ttcttgacag agggcttgac cttcatgttc ttccttcgcg ctgtcttcgt accgcgaccc
     3121 ggcgggggtc acggccgtta ctttccaacg atggctcacc gatagcgata gacgatgcgg
     3181 ccgcgggtca gatcgtacgg gctcagttcc accaccacgc ggtcttgcgg aaggatgcgg
     3241 atgtagtgct gacgcatctt gccggatatg cgggcgagca ccttgtgacc gttcgtcagc
     3301 tctacccgga acatcgcgtt ggggagagct tcggtcacat gcccttcgat ctcgatgaca
     3361 ccgtcttttt tggccatagc ctcactgtcg cttgatggac ccatcactgg tcgtgcggga
     3421 tatgacgcct tcgccggcca caacgacggt ggccgcgcgc gggcacagca atgcccagcg
     3481 tgagacgcca acgatcaata ctacgcgatc tggcaggccc ggcgcaaacc ggccggcgtc
     3541 atgcggggcg caccggcacg atgccgaact cggcgagttc gcgtgcgccg ccgtcggcgg
     3601 cggtcagcac ccaaatgccg tctttgtgaa ctgcgacggt gtgctcccag tgcgccgcgt
     3661 cgccgtggtc gtcggtgacg acggtccacc cgtcggggcg caccgaggtc gcgggactgc
     3721 cctgggtgat catcggctcg atcgcgacga cgaggcccgg gcgcacctcc ggccccttct
     3781 gcttgacccg atagttcggc acgggcgggt cctcgtgcat gtgccggccg atgccatggc
     3841 cgacgtagtc ggtcaagacc ccgtaatcgc cctgcgagcg cacgaaccct tcgatcgcgg
     3901 cgcccacctc gtagaggtac gacgccgcgg cgagcgcggc gatgccgtgc cagagggagc
     3961 gtctcgtgac ctcgttcaac cgggcacgcg cggcgacgac atcgggccgg tcctcgtcgg
     4021 gcacgacgaa gctgcgggcg gcgtcgccat tccagccgtt ctggatcgcg ccgctgtcga
     4081 tggagacgag gtcgccggcc cgcaccaccc gggtgccggg gatgccgtgc accacctcgt
     4141 cgttgaccga tgcgcagacg gtgttgtggt agccgggctc caaaacgaag ttcggctccg
     4201 cgccgaggtc gcgaatggcc cgctcggcga gccggtccag ctcccccgtc gtgatgccgg
     4261 gacgcagggc cgcctcgcag gcgtcgaggg aggcggcggt cgcgaggccg ggcgggatca
     4321 tgagccgaag ctgcgccggc gtcttataga tggtgtgacg catgccgcgc attcaggcgt
     4381 tcgcccccac gccgttctgg agaagcgcgt cgagcacccg gcccaacacc tcgtcggggc
     4441 tgccgagccc gtcgacctcg gccagaagac cacggtcgcg gaagaggccg agaatcggcg
     4501 ccgtctgctc gcgatagacg gcctggcggt gtcggatgcc ctcgtcggtg tcgtcggaac
     4561 ggccctcttc ttcggcccgg tgtcggagcc gccgcacca
//
LOCUS       DASTNL010000061         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1343682, whole genome shotgun sequence.
ACCESSION   DASTNL010000061 DASTNL010000000
VERSION     DASTNL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343682"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFQ96_02125"
     CDS             <1..736
                     /locus_tag="VFQ96_02125"
                     /inference="COORDINATES: protein motif:HMM:NF037316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEU0256620.1"
                     /translation="AAELAAPPAAPRRDPWRRLSGIHAVKGRRRLAVARALWGARDGL
                     ARQKDVSPGRVLPDSALVAAAMAMPASRHDLLGIKGFTGRGGHSHADLWWAAVEEGRT
                     TDDLPSLKKADDATPPARSWKHHNAAAAARLDAAKAALRLIGEEQRIPVENLVRPATV
                     RALAWQPPEEPTVATVDRFLAEHDARRWQRALVAAVLSQAMHAVPAAPDELDSRGPAA
                     GPEDSAAGAAAPEPALAPAASAPPAA"
     gene            775..1962
                     /locus_tag="VFQ96_02130"
     CDS             775..1962
                     /locus_tag="VFQ96_02130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU0256621.1"
                     /translation="MTDVMFIDGARTPFGRAGQKGVYWQTRADDLVVKALLGLMARNP
                     GVPAASVDDVAIAATTQSGDQGLTLGRTCALLAGFPPSVPGFAIDRMCAGAMTSAAML
                     GSAIGFGADDIVVAGGVEHMGRHPIGQDATPNPRFLTERLIDPDGFSMGATAERLHDR
                     FPALTKERADRFGMRSQQKMAAAYAAGKIQPDLVPVAAPSDSGWQLVTEDEGMRPETT
                     MAALAGLATPFRPGGRVTAGTAAPLTDGATVCLLASAKTAKKRGLTPKMRLVGFAFAG
                     VEPELMGLGPIPATERALERSGLRFDDLGLIEVNEAFAVQVLAFLDHFGLGDDDPRVN
                     VWGGALAIGHPLAASGVRLMIQLAAQFAERPDVRYGLTTMCVGLGQGGSMIWENPHWR
                     RRR"
     gene            1959..>2334
                     /locus_tag="VFQ96_02135"
     CDS             1959..>2334
                     /locus_tag="VFQ96_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEU0256622.1"
                     /translation="MTQTPTPEEEFAPLVALAAGEVITHALPNDVRLADGRTLVLVTL
                     DNGVERPNTFGPRGLLELRGVLAEVARRAAAGEVAAVAVTGKPAMFAAGADVTAPIPP
                     DDAAAALLPRLGHETFSMLHSCG"
BASE COUNT          334 a          874 c          819 g          307 t
ORIGIN      
        1 cgccgcggaa ctcgcggcac ctcctgccgc accccgccga gacccctggc ggcgactctc
       61 cggcattcac gcggtgaagg gtcgccgcag gctcgccgtc gcccgagccc tctggggcgc
      121 cagagacggc ctggcgcgcc agaaagacgt ctcgccgggg cgcgtgctgc ccgattccgc
      181 gttggtcgcc gcggcgatgg ccatgcccgc ctcacggcac gacctcttgg gcatcaaggg
      241 attcaccggc cgcggcggcc acagccacgc agacctctgg tgggcggccg tcgaggaggg
      301 ccgcacaacc gacgacttgc cgagcctcaa gaaggccgac gacgcgaccc ctccggcgcg
      361 ctcctggaag caccacaacg cggccgcggc cgcgcggctc gacgccgcca aggccgcact
      421 gcggctcatc ggcgaggaac agcgaatccc cgtggagaac ctcgtgcgcc cggcgacggt
      481 gcgggcgctg gcttggcagc cgcccgagga gccgaccgtc gccaccgtcg accgcttctt
      541 ggcggagcat gacgcgcgcc ggtggcagcg tgctctcgtc gccgccgtct tgtcgcaggc
      601 catgcatgcc gtgcccgcgg cgcctgacga gctcgatagc cggggccctg ccgccggccc
      661 ggaggatagc gcggccggcg cggcggcgcc ggaacccgcg ttggcacccg cggcgtcggc
      721 accacccgcc gcctagtatc gaggaaccgc gtgccgtagg cgaggaggct gacgatgacc
      781 gacgtgatgt tcatcgacgg ggcgcgcacg cctttcggcc gtgccgggca gaagggcgtc
      841 tactggcaga cccgcgccga cgacctcgtc gtcaaggccc tcttgggcct catggcgcgt
      901 aatcccggcg tgcccgccgc atccgtggat gacgtcgcga tcgcggcaac cacgcagagc
      961 ggcgaccagg ggctcacact cggccgaaca tgcgcactcc tggccggatt ccccccttcg
     1021 gtgcccggct tcgccatcga ccggatgtgc gcgggcgcga tgacgagcgc cgccatgctc
     1081 ggctcggcga tcgggttcgg cgccgacgac atcgtcgtgg ccggcggcgt ggaacacatg
     1141 ggccggcacc ccataggcca agacgccacc ccgaacccgc gcttcctcac cgagcggctt
     1201 atcgaccccg acgggttcag catgggtgcc acggcggagc ggctgcacga ccgcttcccc
     1261 gccttgacga aggagcgcgc cgaccggttc ggcatgcgca gccagcagaa gatggcggcc
     1321 gcctacgccg cgggcaagat ccagcccgac ctcgtcccgg ttgccgcgcc ctccgatagc
     1381 ggctggcagc tggtgacgga agacgagggg atgcgccccg agaccaccat ggcggccctg
     1441 gccgggctcg ccacaccgtt ccggccgggc ggccgggtga ccgccggcac cgccgcgcca
     1501 ttgaccgacg gcgcgaccgt gtgcctcctc gcctcggcca agacggcaaa aaagagaggc
     1561 ctcacgccca agatgcggct cgtcggtttc gccttcgccg gcgtggagcc ggaactgatg
     1621 ggtctcggcc cgatccccgc caccgaacgg gccctcgaac gcagcggcct gcgtttcgac
     1681 gacctcggcc tcatcgaggt gaacgaggcc ttcgccgtgc aggttctcgc tttcctcgac
     1741 catttcggcc tgggcgacga cgacccgcgg gtgaacgtgt ggggcggcgc cctggcgatc
     1801 ggccacccgc tggccgcctc gggcgtgcgc ctcatgatcc agctcgccgc ccagttcgcc
     1861 gagcggcccg acgtgcgcta tggcctgacg acgatgtgcg tggggctcgg gcagggcggc
     1921 agcatgatct gggagaaccc acattggagg cgcagacgat gacgcagacc ccgacgccgg
     1981 aggaggaatt cgcgccgctg gtcgcccttg ctgccggcga ggtgatcacg cacgcgctgc
     2041 cgaacgacgt gcggttggcc gacggacgca ccctcgttct cgtcacgctc gacaacggcg
     2101 tggaacggcc gaacaccttc ggcccgcggg ggcttctcga gctgcgcggc gtcttggcgg
     2161 aggtggcccg tcgggcagcg gcgggcgaag tcgccgcggt ggccgtcacc ggcaagcccg
     2221 ccatgttcgc agccggtgcc gacgtgaccg cgccgatccc gcccgacgac gccgccgcgg
     2281 ctcttctgcc gcgcctcgga cacgagacgt tcagcatgct gcactcttgc ggcg
//
LOCUS       DASTNL010000062         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_972297, whole genome shotgun sequence.
ACCESSION   DASTNL010000062 DASTNL010000000
VERSION     DASTNL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972297"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFQ96_02140"
     CDS             complement(<1..565)
                     /locus_tag="VFQ96_02140"
                     /inference="COORDINATES: protein motif:HMM:NF045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256623.1"
                     /translation="MTLSLLDRAIYSYSDVDRLVGLHSGTARRWLEGYERSGRFYDPV
                     LRPEPTGAQVVTWGEMVEARLLAEFRSRRVPVQRLRPAIVRLRHEFGRYPLAHARPFL
                     EVEGRELVRAVQEATGVDRLLRFVVVRNGQAMLAEATRRFSDAVQYDGGVVARLNPSP
                     RTPVVLMDPARTFGQPAIRNVRTETLAE"
     gene            complement(662..737)
                     /locus_tag="VFQ96_02145"
     tRNA            complement(662..737)
                     /locus_tag="VFQ96_02145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(702..704),aa:Thr,seq:cgt)
     gene            complement(812..1204)
                     /locus_tag="VFQ96_02150"
     CDS             complement(812..1204)
                     /locus_tag="VFQ96_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0256624.1"
                     /translation="MILDTGVLIAGVRGRVDVAVLADSDDVAVPAVVVAEYLAGTLLD
                     RDPGRAAAQRAFLEEVLRVVPVCDYDRAVADHHAALLSHVRRLGTRRGAHDLIVAATA
                     RATGRTILTTDERARFGDLPDVTARLFG"
     gene            complement(1201..1467)
                     /locus_tag="VFQ96_02155"
     CDS             complement(1201..1467)
                     /locus_tag="VFQ96_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU0256625.1"
                     /translation="MREMTASEASRRFSAVLDRAERGETVIVTRAGRRVAVIGPSPRA
                     NGEALRAVFDRWRGEPALDDEFAERVSQVREVASSESDVDPWRE"
     gene            complement(1472..2533)
                     /locus_tag="VFQ96_02160"
     CDS             complement(1472..2533)
                     /locus_tag="VFQ96_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0256626.1"
                     /translation="MRRRIDDLEALDVLDAVARCGSMSAAARECRLSQQAVSARIRDI
                     ERRVGFALFERSPRGVALTAEGESVVAWSREVLVAAARLDAAVDLMHRPVQHTLTVGG
                     SQTIAAELLPGWLLGLRQRQLQTGEAPTPVRLRTGNSAEVARLVASGELDVGFIESPA
                     VPRRLSSVTVATDRLVAVVAGSHPWAAARAVRPASERAPEHAREGAEGRGEKHAQEYA
                     WQPQRSEARSRGVTLAEVAATPLVTREQGSGTRLAYETAVRERLAVRPADPALVLGTS
                     VAVLHAVAGGVAPAVLSELAVADEAALGRVCRIPIVGDPITRPLTALWSWGVDGLRGA
                     ARLLVDLAQQEHDHQNLIF"
     gene            2627..>2899
                     /locus_tag="VFQ96_02165"
     CDS             2627..>2899
                     /locus_tag="VFQ96_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256627.1"
                     /translation="MTLQNIACYQEELDGRTMGCVPLSPTPRHTGHPVMPSTRHPAGP
                     GRDPHANPNRLAARRGRFTALGARITRRSAWLAARAREVWPGVALCA"
BASE COUNT          504 a         1080 c          932 g          383 t
ORIGIN      
        1 cctccgcgag cgtctcggtg cgcacattcc ggatagcagg ctgcccgaag gtgcgcgccg
       61 gatccatgag aacgaccgga gtccgagggc tcggattcag ccgagccacg acgccgccgt
      121 catactgaac ggcgtcactg aaccgccgtg tcgcctccgc gagcatcgcc tgtccattgc
      181 gtacgacgac gaaacgtagc aggcgatcca ctccggtcgc ttcttgcacc gcgcgcacca
      241 actctcgccc ttcgacctcg aggaaggggc gggcgtgcgc aagcgggtag cggccgaact
      301 cgtgacggag ccgaacgatc gcagggcgca gacgctgcac aggaacacgc ctgctgcgga
      361 actcggcgag cagccgagcc tcaaccatct ctccccacgt cacaacctgc gcacccgtgg
      421 gttccggacg caagaccggg tcgtagaagc gaccggaacg ctcgtatcct tcgagccacc
      481 gccgtgcggt gcccgagtgc aggccgacga ggcgatccac gtctgagtac gaatagatcg
      541 cccgatcgag caaggaaagg gtcacgccct catgatggca tcatgcgccg acgtttcgct
      601 gtaacgctcg acaggtaggc ccgtcgagat tcccaccgat gcgcacagcc gcccaatggc
      661 atggagccgc ctgtcggaat cgaaccgacg acctattcat tacgagtgaa tcgctctacc
      721 gactgagcta aggcggcgcg gcatgcggcg gcgcatgcac aatgagacat cttagcggat
      781 gcgccgaagc accggccgac gcccgtaggc gctacccgaa cagtcgggcg gtcacgtcgg
      841 gcaggtcgcc aaaccgggcg cgctcatcgg tcgtgaggat cgtgcggccg gtggcgcggg
      901 ccgtggccgc gacgatcaga tcgtgtgcgc cgcggcgcgt gccgaggcgc cgaacgtgag
      961 acagcagtgc ggcatgatga tccgcgacgg cacggtcgta gtcgcagacc gggaccaccc
     1021 gaagcacctc ctcaagaaag gcgcgctgtg cggcggcccg gccgggatcc cgatccagca
     1081 gcgtaccggc gagatattcg gccaccacga cggccggcac cgcgacgtcg tcagagtcgg
     1141 ccaatacggc cacatcgacg cgcccgcgca ccccggcgat aagcacgccg gtgtcgagga
     1201 tcactcgcgc cacggatcca catccgactc agacgaggcc acctcgcgca cctgggagac
     1261 ccgctctgcg aactcgtcat ctagcgcggg ctcaccgcgc cagcgatcga acacggcccg
     1321 cagcgcctca ccattcgccc gcggcgacgg cccgatcact gccacccgcc gccccgccct
     1381 ggtcacgata accgtctcgc cccgttcagc acgatccagc acggccgaga atctccgcga
     1441 cgcttccgag gccgtcatct cccgcattgt ttcagaatat cagattctga tggtcatgct
     1501 cctgctgagc gaggtcgacg agcaggcgtg cggcgccgcg cagcccgtca acgccccacg
     1561 accacagcgc ggtcaggggg cgagtgatgg ggtcgcccac gatcgggatt cggcacacac
     1621 gaccgagagc cgcctcgtcg gcgacggcca gctcgctgag cacggccggc gcgactccgc
     1681 cggcgaccgc gtgcagcaca gccacgctcg tgcccaaaac cagggcaggg tccgcaggcc
     1741 gcaccgccag ccgctcgcgc acggccgtct cataggccag gcgggtgccg ctgccttgct
     1801 cccgagtgac gagcggcgtc gcggcaacct ccgccaacgt cacacctcga gagcgggcct
     1861 ctgatcgctg aggctgccag gcgtactcct gggcatgctt ttcgccccgc ccttcggcac
     1921 cctcccgggc gtgctctggg gcgcgttccg aggcgggtct cacggcgcgt gccgcagccc
     1981 agggatgcga gcccgcgacg accgcgacga gccgatccgt cgcgaccgtc accgacgaca
     2041 gccggcgcgg cacggccggg ctctcgatga aaccgacatc gagttccccc gacgcgacga
     2101 gccgcgcgac ctcagcgctg ttgccggtgc gcagacgcac gggcgtgggc gcctcgccgg
     2161 tctgcagctg acgctggcgc aacccgagca gccagcccgg cagaagctcg gccgcaatcg
     2221 tctggctgcc gccgaccgtg agcgtgtgct gcacggggcg atgcatcagg tcgacggccg
     2281 cgtcaagccg ggctgccgcg accagaacct cccgcgacca cgccaccacc gactcgccct
     2341 ccgccgtcag cgcgacgccg cgcggcgaac gttcgaatag ggcgaacccc acgcggcgct
     2401 cgatgtctct gatacgggcg gagaccgcct gctgcgacag gcggcactcc cgggccgccg
     2461 ccgacatgct gccgcagcgc gccacggcgt caagcacatc caacgcctcg aggtcgtcga
     2521 ttcgccgccg cattccgccc ccctacaatc ctgctctgta gtgtgacggg ctgccgccgg
     2581 cggggagtgc cggcgatccc gattcgtcac catacaacgt gtgtttgtga cgctacagaa
     2641 cattgcctgc taccaagaag agctcgacgg tcgaacgatg ggatgcgtgc cgctctcccc
     2701 caccccacgc cacaccggcc accccgtgat gcccagcacg agacaccccg cgggcccggg
     2761 ccgggacccg catgccaacc ccaaccggtt ggcggccagg cgtggccgat tcaccgccct
     2821 cggggcccgg atcacacgac gaagtgcatg gctcgcggcc cgcgcccgcg aggtgtggcc
     2881 gggcgtcgca ctctgcgcc
//
LOCUS       DASTNL010000063         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_787655, whole genome shotgun sequence.
ACCESSION   DASTNL010000063 DASTNL010000000
VERSION     DASTNL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787655"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1070
                     /locus_tag="VFQ96_02170"
     CDS             195..1070
                     /locus_tag="VFQ96_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEU0256628.1"
                     /translation="MRAYGGDLQELAEYCLAQGIAECEGLRLDVVRAWLGLGAEAGWA
                     KTTLARRSASVRAFSRWLARQADITVPGIDRLRTPRIGRSLPRVLTSEQMADLVGRMA
                     AAAQGGTARAVRDRAIIELLYASAMRVSELTGLDRADVDLARLTVRVVGKGGKERVVP
                     FGVPAARALDDYLDRSRPRLAVDDADPAFFLGVRGRRIGVRVVYRIVNRLLGETSEPA
                     WPGASEEQGVRVHRPSGPHTLRHTAATHMLDGGADLRAVQEMLGHASLGTTQIYTHVS
                     AARLAEAYRLAHPRA"
     gene            complement(1136..1675)
                     /locus_tag="VFQ96_02175"
     CDS             complement(1136..1675)
                     /locus_tag="VFQ96_02175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0256629.1"
                     /translation="MALVAAPGGALAEPRAATPHTLVTAAPAVSQAPGQTPASAEPEP
                     GAGAWQWPLPTRHVLRGFEAPATRYGAGHRGIDLPAAPGARVVAPAGGVVLFDGPVAG
                     RGVITIEVSLGLLLSMEPVTGGPVAGEVVSAGAPVGDIGSGGHCAGCLHLGVRLDGEY
                     VSPLNYLAGVPRAVLLPLH"
     gene            2129..3043
                     /gene="rpsB"
                     /locus_tag="VFQ96_02180"
     CDS             2129..3043
                     /gene="rpsB"
                     /locus_tag="VFQ96_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0256630.1"
                     /translation="MAVVTMRQLLDSGVHFGHQTRRWNPKMKRFILTERSGSHIIDLQ
                     QSLTYIDSTYRFVKDLVARGGTVLFVGTKKQAQNAIAEQAQRVGQPYVNQRWLGGLLT
                     NFQTVSKRLARMKELEELDYEGTTSGFTKKELLLKKREMDKLHKSLGGIRNLSRTPSA
                     LWVVDTKKEHLAVDEAGKLGIPVIAILDTNCDPDEVQYPVPGNDDAIRSVALLTRIVA
                     DAAAEGLIERHKKAGDADAPVEPLAEWESELLASADGTTAEAQQAETATGAGEAEQAS
                     APESPEQQADAAPPAEPAASTEPAAQAE"
     gene            3069..>3275
                     /locus_tag="VFQ96_02185"
     CDS             3069..>3275
                     /locus_tag="VFQ96_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU0256631.1"
                     /translation="MAKVSIADIKALRERLGTGMVATKKALEEADGDVDKAVELLRLK
                     GVKDVAKRATRTAANGLVAAALDGT"
BASE COUNT          530 a         1187 c         1161 g          397 t
ORIGIN      
        1 ccggccgtga cgaggaggcc ctcggccgca tcggccggcc gggcacccga cgactccggc
       61 ggtacccggg ccggcggcga gtcgcccggc ggcgcacgag gcgggtgggc gagactgtca
      121 gcgtgcgatg ggatgcggcc attgcggcct tcacccgcca cctgcgggca gagcgcggct
      181 actcgctcgc caccgtgcgt gcctacgggg gtgacctgca ggaattggcg gagtactgcc
      241 tggcgcaggg gatcgccgag tgcgagggcc tgcggctcga cgtcgtccgc gcctggctgg
      301 gccttggcgc cgaagcaggc tgggcgaaga ccaccctggc caggcgttcg gccagcgtcc
      361 gcgcgttctc ccgctggctg gcgcgtcagg ccgacatcac ggtgcccggc atcgaccgcc
      421 tgcgtactcc tcgtatcggg cgcagcctcc ccagggtgtt gaccagcgag cagatggccg
      481 acctcgtcgg acgcatggcg gccgcggcgc agggtggcac ggcccgcgcg gtgcgcgatc
      541 gggcaatcat cgagcttctc tacgcttctg cgatgcgcgt cagcgaactt accggcctcg
      601 accgcgccga cgtcgacctc gcccggttga ccgtgcgggt cgtgggcaag gggggcaaag
      661 agcgcgtcgt gccgttcggc gttccggccg ccagggccct cgacgactac ctcgatcggt
      721 caaggccccg tctcgcagtg gatgacgcgg atcccgcgtt cttcctcggc gtgcgtggca
      781 ggcggatcgg ggttcgcgtg gtctaccgca tcgtgaaccg gctcctcggc gagacgtcgg
      841 agcccgcgtg gcccggcgcc tcggaagagc aaggcgtgcg ggtgcaccgc ccttccggcc
      901 cgcacacgct gcggcacacc gcggcgaccc acatgctcga cggcggtgcg gacctccggg
      961 ccgttcaaga gatgctcggg catgccagcc tcggcaccac gcagatctac acccacgtct
     1021 ccgcggccag gctcgccgag gcgtatcggc tggcacatcc acgcgcctga gacggcccgg
     1081 ccttctacag cggcgatcgt gccgggcccc ggccggtggg ggccgttcgg cgctgtcagt
     1141 gcaagggcag aagcaccgcc cgcggcacac ccgccagata gttcagcggc gagacgtact
     1201 cgccgtcgag gcgcacgccg aggtgcaggc atccggcaca gtgcccaccg gagccgatgt
     1261 cgccgaccgg ggcgccggcg ctcacgacct cgcctgccac gggcccgccc gtcaccggtt
     1321 ccatggaaag gaggaggccg agcgagacct cgatcgtgat cacgccccgg cccgccaccg
     1381 ggccgtcgaa gaggacgacc ccgccggccg gcgcgacgac ccgggcgccc ggcgcggccg
     1441 gtagatcgat gccgcggtgc ccggcgccat aacgcgtcgc cggcgcctcg aaccctcgaa
     1501 ggacgtgccg tgtcggtagc ggccactgcc aggcgccggc accgggttcg ggttctgccg
     1561 aggccggcgt ctgtcccggc gcctgcgaca cggccggtgc cgccgtaacc agggtgtggg
     1621 gcgtggcggc acggggctcg gccagcgcgc cgccgggggc ggccacgagt gccacgcaca
     1681 ggcaaaaggc cgcgacccct gcccggggcg ctcgcccgcc gccgccccga ggtcgcgtcg
     1741 ctcgccccga tcccacgtcg tctcctcctc gtcagtcatc ggaaccgact cttcctcgcc
     1801 cggcgagcgg ggcggcccgg gggcgcccaa caggggaacg atgctcgtgc gggagcgcgg
     1861 tgcaggtcgc ggccggggag ggggcgcagg cgggcccgga cgtcggccgg gcttcggcgc
     1921 gatgatacga tgaggcccgc accacgtgtg agcgtggtga ctacgcgcgc ccttcacgcg
     1981 ccgcgcatcc aacggtcctg cgatacgccg ccttgacaag gcggaagcgg gcgggtgccg
     2041 gccgggcgcc aggaggcgga cgatccggtt cgccacacac aaccgaatgc ggcaaggcct
     2101 agccctgcca cggagaagga gaacggcaat ggccgtcgtc accatgcgcc agctgctcga
     2161 cagcggcgtc cacttcgggc accagacccg tcgctggaac cccaagatga agcgcttcat
     2221 cctcacggag cgaagcggca gccacatcat cgacctgcag cagtctctga cctatatcga
     2281 cagcacctac cggttcgtca aagaccttgt cgcccgcggc ggcacggtgc tcttcgtcgg
     2341 caccaagaag caggcgcaga acgccatcgc cgagcaggcg cagcgcgtcg gccagcccta
     2401 cgtcaaccag cgttggctcg gcggcctcct cacgaacttc cagaccgtct ccaagaggct
     2461 cgcccgtatg aaggagctcg aagagctcga ctatgaggga acgacgagcg ggttcacaaa
     2521 gaaggagctc ctcctcaaga agcgtgagat ggacaagctc cacaagtcgc tgggcggaat
     2581 ccgcaacctg tcacggaccc cgagcgcgct ctgggtcgtc gacacgaaga aggagcacct
     2641 ggccgtcgac gaggcgggca agctcggcat ccccgtgatc gcgatcctcg acacgaactg
     2701 cgaccccgat gaggtgcagt acccggtgcc cggcaacgac gacgccattc gctccgtcgc
     2761 cctcctgacc cgcatcgtcg ccgacgccgc agcagaaggc ctcatcgaac ggcacaaaaa
     2821 ggcgggcgac gcggacgcgc ccgtcgagcc cctcgccgag tgggaatcgg agttgctcgc
     2881 ctctgccgac ggcaccacgg ccgaggccca gcaggccgaa acagccaccg gcgccgggga
     2941 ggcggaacag gcgtcggcac cggagagccc ggagcagcag gccgatgccg ccccgccggc
     3001 cgagcccgcc gcatccaccg aaccggccgc gcaggccgag tgacccggcc taccctcagg
     3061 agcagaacat ggcaaaagtg agcatcgccg acatcaaggc gctgcgcgag cggctcggca
     3121 ccggaatggt ggcgacgaag aaggcactcg aagaggccga cggcgacgtc gacaaggcgg
     3181 tcgaactgct gcgcctcaag ggcgtgaagg acgtggccaa gcgtgccacc cggacggcgg
     3241 ccaacgggct ggtcgcggcc gcgctcgacg gcacc
//
LOCUS       DASTNL010000064         6265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_47337, whole genome shotgun sequence.
ACCESSION   DASTNL010000064 DASTNL010000000
VERSION     DASTNL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47337"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFQ96_02190"
     CDS             <1..296
                     /locus_tag="VFQ96_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU0256632.1"
                     /translation="VHTAVGGDVEVTDDGRLVIPGTSFLAGASDTLAHGVARLASLPS
                     FSLAQALRLATRNPARVVGDTSDAALRPGTRNVMTFRWRPGDASLKDVQLVEG"
     gene            complement(308..1897)
                     /locus_tag="VFQ96_02195"
     CDS             complement(308..1897)
                     /locus_tag="VFQ96_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU0256633.1"
                     /translation="MGFLPPLLPLPNEVRQTGEGFELTAATTVAAESHLVGVAVQLQN
                     MLRPATGLPLRLVGASVPGSALRLYRDSNLAAEGYRLEVGPDGVRVAGGSAAGVFYGC
                     QALRQLLPAEVFRSARVPGVTWSLPGVHIADAPRWAWRGMMLDVARHFIPKPAVLRMI
                     DLMAAHRLNVLQLHLTDDQGWRLEIRRYPRLTDAGAWRRSSQVGAGEAAPQDGRPHGG
                     YYTQADAREIVAYARERAITVVPEIDAPGHAQAVLAAYPELGVTGRPVETSTVWDIGE
                     NVLNVEESTVRFVTDVLDEVMDLFDSPFIGVGGDEYPTVQWQGDPRTRQRMRELRVAT
                     PDGLRAWFLGQLDSHVSAAGRRIVGWEEILEGELTPGAVVASWRGMAGARAAALAGHD
                     VVSCPDERAYLDYRESDAPDEPVPVSVPVTVRDVYELDPRPQSLPADAAAHILGGQAN
                     VWTEHMDSPRVVDYHVFPRLCALAEALWSRRKDWPGFERRLRGHLRRLDAAGVEYRHS
                     GGPLPWQRRPGVAGWPRERQG"
     gene            complement(2006..3343)
                     /locus_tag="VFQ96_02200"
     CDS             complement(2006..3343)
                     /locus_tag="VFQ96_02200"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0256634.1"
                     /translation="MTQALSTEPITTLGPGHKGLAPSAWGMSQEEFVDSRPRLGDQQT
                     PLLTLDKAALEHNVAEMFAWAEGAGLTLAPHAKTTMAPVLWAALLEAGAWGVTVATGW
                     QAQVARSFGITHVMVANALADPVAVRWAADEMNRDPTCEILSWVDDSRTISAMEKVLR
                     QAGAERKMPVIVELGAMHGRTGVRGVRRAIELARQVHDSRLLELAGVGGYEGVLAHDR
                     SAVSLARVDAYLDDIATLHRSVSQCDLLRDASPIVTAGGSAYFDRVAARLGPLSDAAI
                     VLLRSGAFQLHDDGFYAGISPMGRLPGTRPLRSAMHVRTRVLSHPEPHLALLDAGKRD
                     LPFDEGLPVPLFVVGVSEDESRRLLAGARVSALNDQHAFLQLPGGLADDLPPGTVVVL
                     GLSHPCTAMDRWRLIPIVERAAGRAPAGPSAGGTVPACVYDDTVITALAETYF"
     gene            3592..4044
                     /locus_tag="VFQ96_02205"
     CDS             3592..4044
                     /locus_tag="VFQ96_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0256635.1"
                     /translation="MAELKLEELSASNIAAANSLTLKPGQEQFVAPVSYSVAASVADP
                     NTSWQRVVVLDGRVVGFIHGNFDPDDPHEEFRACIWRINVDAEVQGQGVGSFAARALA
                     EEARKRGFERITVIWEPGTEGPEAFFHRIGFKDVGETRYGETIGALTL"
     gene            4028..4390
                     /locus_tag="VFQ96_02210"
     CDS             4028..4390
                     /locus_tag="VFQ96_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU0256636.1"
                     /translation="MPSHSERGPAGHVSPHVCEDEFAEEVLHVVAQIPPGRVMTYGGI
                     AAQLGSRASRRVGKVMAHYGSAVPWWRVVHADGSAPQCHDGTALRHYVRENTPLRGSA
                     ERFRIVMREAAWRPPDDA"
     gene            complement(4435..5655)
                     /locus_tag="VFQ96_02215"
     CDS             complement(4435..5655)
                     /locus_tag="VFQ96_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256637.1"
                     /translation="MSSTAEATTPTMSTARRRPAAPAVGYAGRLLAAVVTAFCVTAAG
                     YIATIVLLFAANGASAAALASLGAYFALGALVTAIVLFIAAAFGLFARWYGALLAGLV
                     GTPIGVLLGLLLVLSGAKFTGSLWLQMLGSLLSVNFLLWFTVTAAAPLLGPIVWAATV
                     RGRRRSGPVLLVRLPAAGEGAGGAADADEQWDGLVHTLTGLGADAVEVPVAEHSADSV
                     FVGDIVVVLGAAAIPAAGGGRQAEVGDLRLRLRERSAAIEELDEPEDLFGADVLVAGR
                     DVYVAAGGATSAAGIRELREIAAVRGYGVVALEGVTGRLADAATALPDGTVLGDPERL
                     GQAAELVRALRPAPEPAGASVLVVDEQTVLVSAAAPQTQAMLERLGFATVSVDVSAFE
                     SRGGGLGRLAARLG"
     gene            complement(5803..>6265)
                     /gene="purH"
                     /locus_tag="VFQ96_02220"
     CDS             complement(5803..>6265)
                     /gene="purH"
                     /locus_tag="VFQ96_02220"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159103.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0256638.1"
                     /translation="APHDFKQVSGGLLLQTRDSFDGFSSEGWRLVAGPEADEATRADL
                     EFAWRACRSVKSNAILLAHDGASVGVGMGQVNRVDSCGLAIRRAGERAEGSVAASDAF
                     FPFADGVEALVGAGVRAIVQPGGSIRDEEVIAAAAAAGVTMYVTGRRHFFH"
BASE COUNT          982 a         2294 c         2175 g          814 t
ORIGIN      
        1 tcgtgcacac cgcggtcggc ggcgacgtcg aggtgacgga cgacgggcgc ctcgtcatcc
       61 ccggcacgtc gttcctggcc ggtgcaagcg acaccctcgc gcacggcgtc gcccgcctgg
      121 cctccctccc gtcgttctcg ctcgcccagg cgctgcggct cgccacccgg aacccggcgc
      181 gggtcgtcgg cgacacgtcg gacgccgcgc ttcggcccgg cacgcggaac gtcatgacct
      241 tccgatggcg gccgggagac gcgagcctga aagacgtgca gctggtcgag ggctgacttc
      301 aggcccctca cccctgccgc tctcgcggcc agccggcgac cccgggccgc ctctgccacg
      361 gcaggggccc gccggagtgc cggtactcga cccccgcggc gtcgagcctt cgcaggtggc
      421 ctcgcagccg tcgctcgaag cccggccagt ctttacgccg cgaccagagc gcctcggcca
      481 acgcgcacag cctcgggaag acgtggtagt cgacgacgcg cggggaatcc atatgctccg
      541 tccacacgtt cgcctggccg ccgagaatgt gggcggcggc atcggcgggg aggctctgcg
      601 gccgaggatc gagctcgtag acgtctcgca ccgtgaccgg cacggagacc gggacgggtt
      661 cgtcgggggc gtcggactcc cggtagtcga ggtaggcacg ctcgtccggg catgagacca
      721 cgtcgtggcc ggcgagcgcg gcggcacgag cgcccgccat gccccgccac gaggcgacca
      781 cagcgcccgg cgtgagctcg ccctcgagaa tctcctccca gccgacgatg cggcggcctg
      841 ccgcgctcac gtgggagtcg agctggccga ggaaccaggc gcgcaaccca tcgggcgtcg
      901 cgacgcgaag ctccctcatc cgctgccggg tgcggggatc gccctgccac tgcaccgtcg
      961 gatactcgtc gccgccgacc ccgatgaacg gcgagtcgaa caggtccatg acttcgtcga
     1021 gcacgtcggt gacgaagcgc accgtcgatt cctccacatt cagcacgttc tcgccgatgt
     1081 cccacacggt ggaggtctcg accgggcggc cggtgacgcc gagctcgggg tacgcggcga
     1141 gaacggcctg cgcatgaccg ggcgcgtcga tctcggggac gacggtgatc gcgcgttccc
     1201 gcgcgtaggc gacgatctcg cgggcgtcgg cctgcgtgta atacccgccg tgcggccgcc
     1261 cgtcttgcgg ggcggcctcg cccgcgccga cctggctgct gcgccgccac gcacccgcgt
     1321 cggtgagccg cgggtagcgc ctgatctcga ggcgccagcc ctgatcatcc gtcaggtgca
     1381 gctgcagcac gttcaggcga tgggccgcca tgaggtcgat catgcgcagt accgccggct
     1441 tcggtatgaa gtgtcgtgcc acgtcgagca tcatgccgcg ccacgcccaa cgcggcgcat
     1501 cggcgatatg cacgcccggc agagaccacg tgacgcccgg cacgcgagcg gagcggaaga
     1561 cctcagccgg cagaagctgc cgcagcgcct gacagccgta gaacacgccc gcggccgaac
     1621 cgcccgccac acgcacacca tccggcccca cctcgaggcg ataaccctcg gccgcgaggt
     1681 tcgagtctcg gtaaaggcga agggcagagc ctggcacgct cgcgcccacc aggcgcagcg
     1741 gaaggccggt cgccggccgc agcatgttct gcagctgcac ggcgacgccc acgagatggc
     1801 tctcggccgc caccgtggtc gcggctgtca actcgaagcc ttctccggtc tgccgaacct
     1861 cgttcggcaa cggcagcaag ggaggaagaa accccatgat gctccttcgg ctcgaggcgc
     1921 gggccgcccg gcaccggagg gccgccgcac gccgacgccg ctcccacctt aggtgaccga
     1981 tgcccggccg aggcccggat cgcggtcaga agtacgtctc ggcgagcgcc gtgatcaccg
     2041 tgtcgtcgta gacgcaggcc ggcacggtgc cccctgccga ggggcctgcc ggtgcacgtc
     2101 ccgccgcgcg ttcgacgatg gggatcaacc gccaacgatc catcgccgtg cagggatggg
     2161 agagccccaa gacgaccact gtgccgggcg gcaggtcgtc ggccaggccg cccggcaact
     2221 gaaggaaggc gtgctggtcg ttcaacgcgc tcacccgggc tcccgccagc aggcggcgac
     2281 tctcgtcttc actcaccccg acgacgaaaa ggggcaccgg caggccctcg tcgaacggca
     2341 ggtcacgctt gccggcgtcg aggagcgcca agtgcggctc ggggtgcgag agcacccggg
     2401 tgcgtacgtg catggcggag cgcaacggac gggttccggg cagcctgccc atcgggctga
     2461 tgcccgcgta gaacccgtcg tcgtgcagct gaaaggcgcc ggagcgcagc agcacgatcg
     2521 cggcgtcgct gagggggccg agccgggcgg cgacccgatc gaagtaggcg ctgccgcccg
     2581 cggtgacgat gggcgaggcg tcgcgcagca ggtcgcattg gctcaccgaa cgatgaagag
     2641 tcgcgatgtc gtcgagatag gcgtcgaccc tcgccagcga gacggcgctg cggtcgtggg
     2701 cgagtacgcc ctcgtaaccg ccgacgccgg ccagttcgag gagccggctg tcgtgaacct
     2761 ggcgtgcgag ctcaatcgcc cgtcgcacgc cgcgcacgcc ggtgcggccg tgcatggcgc
     2821 ccagctcgac gatgacaggc atcttgcgct ccgcgccggc ctgccgcaga accttctcca
     2881 tggccgaaat cgtgcgtgag tcgtcgaccc agctgaggat ctcgcaggtc gggtcgcggt
     2941 tcatctcgtc ggcggcccag cgcaccgcga cggggtcggc gagggcgttc gccaccatga
     3001 cgtgcgtgat gccgaagctg cgggccacct gcgcctgcca gccggtcgcg acagtgacgc
     3061 cccaggcgcc cgcctccaaa agcgccgccc acaggaccgg cgccatcgtc gtcttcgcgt
     3121 ggggagccag ggtgaggccc gcgccttcgg cccacgcgaa catctcggcg acattgtgtt
     3181 cgagggctgc cttgtcgagg gtgagcaacg gcgtctgctg gtcgccgagg cggggccggg
     3241 aatccacgaa ctcttcttgc gacatgcccc aggccgacgg ggcgagaccc ttgtggcccg
     3301 ggccgagggt cgtgatcggc tccgtcgata acgcctgcgt catccgcagc ctcccgcgtc
     3361 ttggcctcgc cgccgcaccg cggccacagc cttacagtct cgcgcctcat cagcccggcg
     3421 tgtcacaccg taccgccgtc acatgccccg tacacggata ctctagaacc ccggcgcccg
     3481 gcaggcatag gccccctttc acacaccgcc acagtcggta caaatccgat ggaggcccgg
     3541 ccgggcacct ccgctcgtcc acggcatcgg ggcgtcggat actctgaatt catggctgag
     3601 ttgaaactgg aagagctgtc tgcctcgaac atcgcggccg ccaacagcct caccctcaag
     3661 ccgggccaag agcagttcgt cgcgccggtc tcgtattcgg tggcggcctc ggtcgccgac
     3721 ccgaacacgt cgtggcagcg cgtcgtcgtc ttagacggcc gggtcgtcgg cttcatccat
     3781 ggcaatttcg accccgacga cccccacgag gagtttcggg cctgcatctg gcgtatcaac
     3841 gtcgacgccg aggtacaagg ccaaggggtc ggcagcttcg cggcgcgcgc cctcgcagag
     3901 gaggcccgca agcggggctt cgagcgcatc acggtcatct gggagcccgg gaccgaaggc
     3961 ccggaggcct tctttcaccg gatcgggttc aaagacgtcg gagagacccg ctatggcgag
     4021 acgatcggtg ccctcacact ctgagcgcgg ccccgccggg cacgtctcgc cgcacgtttg
     4081 cgaagacgag ttcgccgagg aggtcttgca cgtcgtcgcc cagatcccgc ccggtcgggt
     4141 catgacgtac ggcggcatcg ccgcacagct tggttcccgg gcgagccggc gggtcggcaa
     4201 ggtcatggca cattacggct cagccgtgcc ctggtggcgg gtcgtccacg ccgacggcag
     4261 cgcgccacag tgccacgacg gcacggcgct tcgccattac gtgcgcgaga acacgccgtt
     4321 gcgcggctcg gcagaacggt tccgcatcgt catgcgcgaa gcggcctggc gcccgcccga
     4381 cgacgcctga ctcgacacac gacctacccg gcagggtccc ggcgcccgtt ttcgtcagcc
     4441 gagacgggcg gcgagacggc cgagtcctcc gccgcggctc tcgaacgcgc tcacgtcgac
     4501 cgacacggtg gcgaagccga gccgttcgag catcgcctgg gtctggggtg cggcggccga
     4561 gacgagcacg gtctgttcgt cgacgaccaa gacggaggcc ccggcaggct ccggcgcggg
     4621 ccggagtgcc cgaacgagtt ccgccgcctg accgagacgc tcggggtcgc ccagcaccgt
     4681 gccgtccggc aacgccgtcg ccgcatccgc caaccgaccg gtgacgccct cgagcgcgac
     4741 gaccccgtag ccgcgtacgg ccgcgatctc gcgcagctcg cggatcccgg cagcgctggt
     4801 cgccccgccc gccgcgacat acacgtcgcg gcctgcgacc agcacgtcgg cgccaaaaag
     4861 atcctccggc tcgtcaagct cttcgatcgc ggcggaacgc tcccgcagcc gcagccgcag
     4921 gtcgcccacc tcggcctgcc tgccgccgcc ggcagccggg atggcggccg cccccagaac
     4981 gacgacgatg tcgccgacga agaccgagtc ggcggaatgc tcggcgaccg ggacctcgac
     5041 ggcgtcggcg ccgaggccgg tgagcgtgtg gacaaggccg tcccactgtt cgtcggcatc
     5101 cgccgccccg cccgcaccct cgccggccgc cggcagccga acgagcaaca ccgggcccga
     5161 ccgacgacgg ccgcgcacgg tcgcagccca gacgatcggc cccaagagcg gtgccgcggc
     5221 cgtcaccgtg aaccagagaa gaaagttgac gctcaacaag gagccgagca tctgcagcca
     5281 gagcgatccg gtgaacttcg cgccggagag tacgaggaga agccccagaa gcaccccgat
     5341 cggggtgccg acaagccccg cgaggagagc cccgtaccag cgggcgaaga ggccgaacgc
     5401 ggcggcgatg aagagtacga tcgcggtgac gagcgcgccg agcgcgaaat aggcgccgag
     5461 gctcgcgagc gccgccgcgc tcgcgccgtt cgccgcgaag agcagcacga tggtggcgat
     5521 gtagcccgcg gcggtcacgc agaacgcggt gacgaccgcc gcgaggagcc ggcccgcgta
     5581 gccgaccgcc ggtgccgccg ggcgccgccg ggcggtcgac attgtcgggg tcgtggcttc
     5641 tgctgtgcta ctcacagtgt gaccttaccg gagcattccc tgtgcgatat gtaccgggcg
     5701 aatagtcacg gcgtccgcgc caaaggcagg ccgcacggcg gggcaggagg ccggccgtgc
     5761 ggctcgacgc cgccccgtgg ccgccgggcg ggacgaggcc ggtcagtgga agaagtgccg
     5821 gcgccccgtg acatacatgg tgaccccggc ggcggccgcg gcggcgatga cctcctcgtc
     5881 gcggatggag ccgcccggct gcacgatcgc ccgcacgccc gcccccacga gggcctcgac
     5941 gccgtcggcg aaggggaaga acgcgtcgga ggcggcgact gacccctcgg cccgctcccc
     6001 cgcccggcgg atcgcgagcc cgcacgagtc gactcggttg acctggccca ttccgacccc
     6061 gaccgacgcg ccgtcgtggg cgagaaggat ggcgttcgac ttcaccgaac ggcacgcccg
     6121 ccacgcgaac tccaggtcgg cgcgcgtcgc ttcgtcggcc tcgggcccgg cgacgagccg
     6181 ccagccctcg ctcgagaatc cgtcgaacga gtcgcgggtc tgcagaagca accccccgga
     6241 aacctgtttg aagtcgtgcg gtgcg
//
LOCUS       DASTNL010000065         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1159601, whole genome shotgun sequence.
ACCESSION   DASTNL010000065 DASTNL010000000
VERSION     DASTNL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159601"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VFQ96_02225"
     CDS             <1..947
                     /locus_tag="VFQ96_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256639.1"
                     /translation="NALTHPDLSFRIADRDEAGANALTRLHGRGVNLVAGALARSTDH
                     ILSFFTMLRDELGFYVGCIRAAAALEEHDLPVARPDVRPAGGVLLDAHGLFDVALAVM
                     NRTSVVGNDVAAADDDLIVITGVNSGGKSTLLRAIGQAQLMAQCGMFVPAASLTAAVR
                     HGVFTHFKREEDDELTGGKLDEELARMSAIADQLASGALVLFNESFASTNEKEGSEIA
                     RQVVHALIRRGVKAVYVTHMYDLAHGLATEAEGGRARALFLRAEPARDFRLVEGTPLP
                     TSHGSDLYTRVFGRSPADNDERTTSCATSPSPATAAPR"
     gene            905..1486
                     /locus_tag="VFQ96_02230"
     CDS             905..1486
                     /locus_tag="VFQ96_02230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0256640.1"
                     /translation="MRYVTLARHGRTALNTEGRLRGLADPPLDDEGREEAARLAGDLA
                     GWNPLLVLCSPLHRARDTAWAIAGRGGAELRTDKHFNDRDYGAWTGEVKAEVVARFGS
                     VDAAPGVESLDTVLERARRGLARVVSGRPERVVVVTHDAVIRAVLAWIDPAVEAAASV
                     PTASWCRLAYDDRADAWRVDLVDQKPTSSWSAS"
     gene            1483..1674
                     /locus_tag="VFQ96_02235"
     CDS             1483..1674
                     /locus_tag="VFQ96_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256641.1"
                     /translation="MTDREPVGGADAQQPGRHAPVIVVGLVPHQPERVALEAARFAGL
                     LGAELVCAHVDPERYVVQR"
     gene            complement(1692..2267)
                     /locus_tag="VFQ96_02240"
     CDS             complement(1692..2267)
                     /locus_tag="VFQ96_02240"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEU0256642.1"
                     /translation="MAHRRFTREELERFRDADVPDLVGPGVKLLFIGVNPGLWTAATA
                     TPFAHPGNRFYPALDKAGIVALPKNFADGFSDDDRAHIMAAGIGITNLVHRATVRADE
                     LSREELRAGASRVAEDVARWRPAVVAVVGITAYRTGFRRPKAVRGEQPELLSGARLWV
                     VPNPSGLNAHDTVASLAEAYAEPARAAGLLR"
     gene            complement(2332..>3968)
                     /locus_tag="VFQ96_02245"
     CDS             complement(2332..>3968)
                     /locus_tag="VFQ96_02245"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU0256643.1"
                     /translation="QIRHVTKSGTVTVLRQEIPVTAGEIVDGTVMSRAALERFLTEQI
                     ARAARENVLFSVQLKATMMKVSDPLIFGSAIRVFFPQVFERWGEALGAAGLNPANGLA
                     GILAGLDAVPEGEKIRAAIHERLTREPAIAMVDSERGITNLHVPSDVIIDASMPAMIR
                     NSGKMWNAAGELQDTLAVIPDSSYAGVYQAVIDDCRAHGAFDPATMGSVPNVGLMAQK
                     AEEYGSHDKTFEIEAAGMVQVVDSAGRVLIEHAVEAGDIWRACQAKDVPIKDWIELAV
                     RRSKVSHTPAVFWLDETRAHDAQLIKKVREHWAEIDTDGLELTIMAPVEATRYSLERI
                     RRGLDTISVTGNVLRDYLTDLFPIMELGTSAKMLSIVPLIAGGGLFETGAGGSAPKHV
                     AQLLKEDHLRWDSLGEFLALASSLDHLAETTGNKRAGILGRALDRATGVLLEKNMSPR
                     RVVGELDNRGSHFFLATQWAKELAAQTDDTALAASFAPLAKKLAAAANVIDAELLGVQ
                     GASVDLGGYYRPDEAKTDAVMRPSSTFNDILATLTA"
BASE COUNT          575 a         1345 c         1400 g          648 t
ORIGIN      
        1 tgaacgcgct gacccacccg gatctgtcgt tccgcatcgc tgaccgcgac gaggccggcg
       61 ccaacgcact cacccggttg cacggacgcg gcgtgaacct cgtcgccggt gcgttggccc
      121 gctccaccga ccacatcctg agcttcttca cgatgctgcg cgacgagctc ggcttctacg
      181 tcggctgcat ccgtgccgcg gcggcgttgg aagagcacga cctgccggtc gcacggcccg
      241 acgtgcggcc ggcgggcggg gtgctcctcg acgcgcacgg cctctttgac gtggccctgg
      301 ccgtcatgaa ccgcacgagc gtcgtcggca acgacgtggc ggccgccgac gacgacctga
      361 tcgtcatcac cggggtgaac agcggcggca agtccacgct gctccgggcg atcgggcagg
      421 cacagctgat ggcgcaatgc ggcatgtttg tacccgccgc ctcgctcacg gcggccgtgc
      481 gccacggggt tttcacccac ttcaaacgcg aagaggacga cgagctcaca ggcggcaagc
      541 tcgatgagga attggcgcgg atgagtgcca tcgccgacca gctcgcctcc ggggccctgg
      601 tgctcttcaa cgagtcgttc gcctcgacga acgagaagga gggctccgag atcgcccggc
      661 aggtcgtgca cgcgctcatt cgccgcggcg tgaaggcggt ctacgtcacc cacatgtacg
      721 accttgccca cggccttgcg acagaggctg aaggcggccg cgcccgggcg ttgttcttgc
      781 gggccgagcc ggcacgcgac tttcgcctcg tcgagggcac acccttgcct accagccacg
      841 gcagtgacct gtacacacgg gtcttcggcc gctcccccgc agacaacgac gaaaggacca
      901 cctcgtgcgc tacgtcaccc tcgcccgcca cggccgcacc gcgctgaata ccgaagggcg
      961 attgcgcggc ctggccgatc ccccgctgga cgacgaggga cgcgaggagg ccgcccggct
     1021 cgccggcgac ctggccggtt ggaatccgct gctggtgctc tgcagcccgc tgcaccgtgc
     1081 ccgggacact gcctgggcga tcgctgggcg cggcggtgcc gagttgcgta ccgacaagca
     1141 tttcaacgac cgcgactacg gcgcctggac cggtgaggtc aaggcggagg ttgtcgcccg
     1201 gttcggcagt gtcgacgccg cccccggcgt cgaaagcctc gacacggtgc tggagcgggc
     1261 gcgaaggggg ctggcgcgcg tcgtctccgg ccgtccggaa cgggtggtcg tcgtcacgca
     1321 cgatgccgtg atccgtgccg tcctggcctg gatcgacccg gcggtcgagg ccgccgcgag
     1381 cgtgccgacg gccagctggt gccggctcgc atacgacgac cgcgccgacg catggcgcgt
     1441 ggacctcgtc gaccagaagc ccacgtcgtc atggagcgcg agttgacaga tcgggaaccc
     1501 gtcggagggg ccgacgcgca gcagccagga cggcatgcgc cagtcatcgt cgtcggcttg
     1561 gtcccgcacc agccggagcg cgtagcgttg gaagcggcgc ggttcgccgg gttgctcggg
     1621 gcggagttgg tgtgcgccca cgtcgacccg gaacgctacg tcgtgcagag atagttcggt
     1681 gcggcgggcc gtcagcgcag gaggccggcg gcgcgggcgg gctcggcgta ggcctcggct
     1741 aggctcgcca ccgtgtcgtg cgcgttcagg ccgctggggt tcggcacaac ccaaagccgg
     1801 gcgcccgaga ggagttccgg ctgttcgccc cgtaccgcct tcgggcgacg gaagcctgtg
     1861 cggtatgcgg tgatccccac taccgccacc acggcgggcc gccagcgggc cacgtcctct
     1921 gcgacccgcg aggcgccggc ccgtagctct tcacgactca gctcgtcggc gcgcacggtg
     1981 gcgcggtgca cgaggttcgt gatgccgata cccgctgcca tgatgtgagc acggtcgtcg
     2041 tcggagaagc cgtcggcgaa gttcttgggc agcgcgacga ttcctgcctt gtcgagcgcc
     2101 gggtagaacc ggttgcccgg gtgtgcgaag ggcgtcgccg tggctgcagt ccagagcccc
     2161 gggttgacgc cgatgaagag gagtttgaca ccggggccga cgaggtcggg cacgtccgcg
     2221 tcgcggaacc gctccaattc ctcccgcgtg aaacgtcgat gtgccacctg ccccgtgcct
     2281 cccctctcgt ggtgcgtcgt cgttcgccca tgccgagccc gtcggcgtag gtcaggcagt
     2341 gagggtggcg aggatgtcgt tgaacgtcga cgacgggcgc atgacggcgt cggtcttcgc
     2401 ctcgtcgggg cggtaatagc cgccgaggtc gacggaggcg ccttgtacgc cgagcagctc
     2461 ggcatcgatg acgttcgcgg cggcggcgag cttcttagcg agcggtgcga acgaggcggc
     2521 gagcgccgtg tcgtcggtct gcgccgcgag ctccttcgcc cattgggtgg caaggaagaa
     2581 atggctgcct cggttgtcga gctcgcccac cacgcgccga ggggacatgt tcttctccag
     2641 cagcacaccg gtggcgcggt cgagggcccg gccgaggatg ccggcgcgtt tgttgccggt
     2701 cgtctccgcc aggtggtcga ggctcgaggc gagggcgagg aattccccca ggctgtccca
     2761 gcgcaggtgg tcttctttga ggagttgcgc gacgtgcttc ggtgccgagc cgcccgcgcc
     2821 ggtctcgaag aggccgccgc ccgcgatgag cggcacgatc gagagcatct tcgcgctcgt
     2881 gccgagttcc atgatgggga acaggtcggt caggtagtcg cggagcacgt tgccggtgac
     2941 ggagatcgtg tcgaggcccc ggcggatccg ctccagcgag taccgggtcg cctcgaccgg
     3001 cgccatgatg gtgagctcga ggccgtcggt gtcgatctcg gcccagtgct cccgcacctt
     3061 cttgatcagt tgcgcatcgt gcgcccgcgt ctcgtcgagc cagaacacgg ccggcgtgtg
     3121 cgagaccttc gagcgtcgca cggccagctc gatccaatcc ttgatcggca cgtccttggc
     3181 ctggcaggcc cgccagatgt cgccggcctc gaccgcgtgc tcgatgagca cccggccggc
     3241 ggagtcgacg acctgcacca ttccggcggc ctcaatctcg aacgtcttgt cgtggctgcc
     3301 gtactcttcc gccttctgcg ccatgagccc gacgttcggc actgagccca tcgtggccgg
     3361 gtcgaaggcg ccgtgtgcgc ggcagtcgtc gatgacggcc tggtagacgc ccgcgtagct
     3421 ggagtcgggg atgacggcga gcgtgtcttg caactcaccc gccgcgttcc acatcttgcc
     3481 cgaattacgg atcatggccg gcatcgacgc gtcgatgatg acgtcgctcg gcacgtgcag
     3541 gttcgtgatg ccgcgttctg agtcgaccat cgcgatggcc ggctcccgtg tcagccgctc
     3601 gtggatggcg gcccgtatct tctcgccctc cggcacggcg tcgagccccg cgagaatccc
     3661 ggcgagaccg ttggccgggt tcaggccggc cgcccccaga gcctcgcccc acctctcgaa
     3721 gacttggggg aagaagacgc ggatcgcgct gccgaagatg agcgggtcgg agaccttcat
     3781 catggtggcc ttcagctgaa cggagaaaag cacgttctcc cgtgctgcgc gggcgatttg
     3841 ttcggtgagg aagcgttcga gggctgcgcg gctcatcacc gtgccgtcga cgatctcgcc
     3901 ggccgtgacg gggatctcct gccgcagcac cgtgaccgtg ccagacttcg taacgtgccg
     3961 gatctgca
//
LOCUS       DASTNL010000066         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1345251, whole genome shotgun sequence.
ACCESSION   DASTNL010000066 DASTNL010000000
VERSION     DASTNL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345251"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFQ96_02250"
     CDS             complement(<1..560)
                     /locus_tag="VFQ96_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0256644.1"
                     /translation="MGHHDHSYPPQPSPLAVAVYDNHTHLDPEVSDTDRVGQPLSMHE
                     HLGRASAAGVAGIVQVGTDLESSRWSAEAAARDDRVLAAVAIHPNEAPRLAARGELED
                     ALATIDDLAGRPRVRAVGETGLDWFRTGDDGRAAQVSSFEAHIEIAKRHGLALQIHDR
                     QAHRGVIETLDRVGAPERTVLHCFSG"
     gene            complement(568..2145)
                     /gene="metG"
                     /locus_tag="VFQ96_02255"
     CDS             complement(568..2145)
                     /gene="metG"
                     /locus_tag="VFQ96_02255"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU0256645.1"
                     /translation="MADGSSFFITTPIFYVNDVPHIGHAYTEVAADVLARWHRQAGDD
                     TWLLTGTDEHGQKILRTATANGATPKQWADKLVTEAWHPLLETVDIANDDFIRTTDER
                     HERNVQRFLQKLYDAGYIYAGEYEALYCVGCEEFKPLSETLDGTGEYEGQKVCAIHSK
                     PLELLQEKNYFFRMSKFQDRLLELYRARPDFVQPESVRNEVMQFVRQGLADLSISRSS
                     FDWGIKVPWDPSHVVYVWFDALLNYITAIGYGEDEAAFHRRWPATHIVGKDIVRFHAV
                     IWPAMLMAAGLEVPQKVFGHGWLLVGGEKMSKSKLTGIAPAQITDTFGSDAFRYYFMR
                     AINFGQDGSFSWEDLAARYQAELANGFGNLASRVTAMIARYDDGAVPGAGEPAAADRD
                     VRKTVAEAAAAADAAMNRLAIHEALDAIWTIVDELNGYITAQEPWALAKDPARRGRLD
                     TVLYTTAEALRALTVLLSPVLPKATRRLWSALAEDALGPLADQPVRSAGQWGQLPAGA
                     RVQKIEALFPRIEAVVD"
     gene            complement(2272..3102)
                     /gene="rsmI"
                     /locus_tag="VFQ96_02260"
     CDS             complement(2272..3102)
                     /gene="rsmI"
                     /locus_tag="VFQ96_02260"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU0256646.1"
                     /translation="MIILAGTPIGNLGDASARLRDTLESAAIVAAEDTRVAGRLLHGL
                     GIANRPKLIALHEHNERQRAEELAEEARALDLVVVTDAGMPTVSDPGYRIVEAAARVG
                     VPVTVVPGPSAVLAALAVSGLPTDRFTFEGFISKRAGERAAAFAALAAEERTMVFFDS
                     PHRLAASLAALAEAFGRERRAAVCRELTKLHEEVRRDTLGGLQAWAEEGLRGEIVLVV
                     AGAPAAAGDPDAALARVLELVASGTGLKEASATVAAREGLGRRELYEAALAARRSPRP
                     "
BASE COUNT          471 a         1100 c         1146 g          505 t
ORIGIN      
        1 tcgccggaga agcagtgcag cacggtgcgt tccggagcgc cgacgcgatc aagggtctcg
       61 atgacacctc ggtgcgcctg gcggtcgtgg atctgcagcg ccaggccgtg cctcttcgca
      121 atctcgatat gcgcctcgaa ggaggatact tgggccgccc ggccgtcgtc gccggtgcgg
      181 aaccagtcga ggccggtctc gccgacggcc cgcacccgtg gccggccggc aaggtcgtcg
      241 atggtggcga gcgcgtcctc cagctcaccc cgggccgcga gccggggtgc ctcgttgggg
      301 tggatggcga cggccgcgag cacccgatcg tcgcgggcag ccgcctcggc cgaccagcgg
      361 gaggattcca ggtcggtacc cacctgcacg atgcctgcca cgccggcggc cgacgcccgc
      421 ccaaggtgct cgtgcatgct caggggctgc ccgacccggt cggtgtcgga gacctcgggg
      481 tcgagatggg tgtggttgtc gtagacggcg accgcgagcg gcgaaggctg cggagggtag
      541 ctgtggtcgt ggtggcccat cggggcgtca gtccacgacg gcttcgatgc gcgggaaaag
      601 cgcctcgatc ttctggaccc gggccccggc cggcaactga ccccactgcc cggcggagcg
      661 caccggctgg tcggcgagcg ggccgagcgc atcctcggcg agcgccgacc agagccggcg
      721 ggtcgccttc ggcagtacgg ggctcaagag cacggtgagc gcccgcaacg cctccgccgt
      781 cgtgtagagg acggtatcga ggcggccacg gcgtgccggg tctttcgcga gggcccacgg
      841 ctcctgcgcg gtgatatagc cgttcagctc gtcgacgatc gtccagatcg cgtcgagcgc
      901 ttcgtggatc gcgagccggt tcatcgcggc gtcggcggcc gcggcggcct cggcgacggt
      961 cttgcgaacg tcgcggtcgg cggcagcggg ctcgccggcc cccggcacgg cgccgtcgtc
     1021 gtagcgcgcg atcatcgccg tgacccggga ggcgaggttg ccgaacccgt tggcaagttc
     1081 cgcctgatac cgggcggcaa ggtcttccca gctgaacgag ccgtcctggc cgaagtttat
     1141 ggcccgcatg aagtagtagc gaaacgcgtc ggagccgaag gtgtcggtga tctgcgccgg
     1201 cgcgatgccc gtcaatttcg acttcgacat cttctcgccg ccgaccagca gccagccgtg
     1261 gccgaagacc ttctgcggca cctcgaggcc cgccgccatg agcatcgccg gccagatgac
     1321 ggcgtgaaag cgcacgatgt ctttgccgac gatgtgcgtg gccggccatc gcctgtggaa
     1381 ggcggcctcg tcttcgccgt agccgatcgc cgtgatgtag ttgagaagcg cgtcgaacca
     1441 cacgtagacg acgtggctcg gatcccacgg caccttgatg ccccagtcga agctggagcg
     1501 cgagatcgag aggtcggcca ggccctgccg cacgaactgc atgacctcgt tgcgcaccga
     1561 ctccggctgc acgaagtcgg gccgggcacg gtagagctcg agcagccggt cttggaactt
     1621 gctcatgcga aagaaatagt tcttctcttg caagagctcg agcggtttcg aatggatggc
     1681 gcagaccttc tgaccctcat attcgccggt gccgtcgagg gtttcactca ggggcttgaa
     1741 ctcctcgcag ccgacacagt agagcgcctc gtattccccc gcatagatgt agccggcgtc
     1801 atacagcttc tgcagaaagc gctgcacgtt gcgctcgtgc cgctcgtctg tcgtgcggat
     1861 gaagtcgtcg ttggcgatgt cgacggtctc caaaagcgga tgccacgcct cggtgacgag
     1921 cttgtcggcc cactgcttgg gtgtggcgcc attcgcagtg gccgtgcgca ggatcttctg
     1981 cccgtgttcg tccgtgccgg tcaagagcca ggtgtcgtcg cccgcctgcc ggtgccagcg
     2041 ggcgagaacg tcggcggcga cctccgtgta tgcgtggccg atatgcggaa cgtcgttgac
     2101 atagaagatc ggggtggtga tgaagaaaga cgagccgtcg gccatgcgac ctatcttaaa
     2161 gggaaggcgt tctcgccctg acctcgtgcc aaggcggcct cgtgccgtca cccccgctgc
     2221 ggggcgcgcc gcgatccctc ccgccggcca cgtcgactgg tcgatgcctg ttcacggccg
     2281 cgggcttcga cgtgcggcga gtgccgcctc gtagagctcg cggcggccga gcccctccct
     2341 ggcggcgacg gtggcgctgg cctccttgag ccccgtgccg ctcgcgacga gttccagcac
     2401 gcgggcgagg gccgcgtcgg ggtcgcccgc cgcggcggga gccccggcga cgaccagcac
     2461 gatctcaccg cgcagcccct cttcggccca cgcctgcagc ccgccgagcg tgtcacgacg
     2521 cacctcttcg tgcagtttcg tcagttcccg gcacaccgcc gcccgccgct ctcggccgaa
     2581 cgcctcggcc agcgccgcaa ggctcgccgc cagccgatgc ggggagtcga agaacaccat
     2641 ggtgcgttcc tcggcggcga gcgcggcgaa ggcggcagcc cgctcgccgg cccgcttgga
     2701 gatgaacccc tcgaaggtga aacggtcggt cggcaggccg gagacggcca gggcggcgag
     2761 taccgccgaa gggcccggca cgacggtgac cggcacgccg actcgtgccg ccgcctcgac
     2821 gatgcggtag ccggggtcgg agacggtcgg catgcccgcg tccgtcacga ccacgagatc
     2881 gagagcccga gcctcttccg cgagttcctc ggccctctgc ctctcgttgt gctcgtgcag
     2941 ggcgatgagc ttcggccggt tggcgatacc gaggccgtgg agaaggcgcc cggcgacccg
     3001 cgtgtcctcc gccgcgacga tggccgcgga ctccagcgtg tcacgcagcc tcgccgaggc
     3061 gtcgccaagg ttcccgatgg gcgttccggc gaggatgatc atggcatcag tctgacagtc
     3121 cggcatggct acgatgacga ggtgatgacc acgcggccgg tccctgcccg aggggacgcc
     3181 cgcgggcccg gcccggcgca ccgtgcggcg cggggcgggg ca
//
LOCUS       DASTNL010000067         6802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_234133, whole genome shotgun sequence.
ACCESSION   DASTNL010000067 DASTNL010000000
VERSION     DASTNL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234133"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..484)
                     /locus_tag="VFQ96_02265"
     CDS             complement(86..484)
                     /locus_tag="VFQ96_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0256647.1"
                     /translation="MTSHLVCDASAIVALLLDSGPDGQWSASVLTGTSLAAPTLLAFE
                     AANIIRRHKAAHLITVDQAAQAHADLVALGIEQWPYEVLATRIWELRQNLTSYDAGYV
                     ALAEFLRVPLVTLDRRIGRAPGVRCTIITP"
     gene            complement(481..717)
                     /locus_tag="VFQ96_02270"
     CDS             complement(481..717)
                     /locus_tag="VFQ96_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256648.1"
                     /translation="MGVSMTVRDVPDAVRDELAARAARAGQSLQEYVRGMLVDSASRP
                     SAGDVIARARARVDATGVRVDARSILVARDADRR"
     gene            complement(946..2316)
                     /locus_tag="VFQ96_02275"
     CDS             complement(946..2316)
                     /locus_tag="VFQ96_02275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0256649.1"
                     /translation="MTATAHGPRDPGALPVDPESKARLARDGLRLGLVDTSSASFALW
                     LQAGARGFHNARPTREEVGRVTPESAYRRTTAVWDDAADPGTPVATVSSWVSPLSVPG
                     PLGPTLDVDAWAISSVTVSPTHRRHGIARALLEAELRTAHAAGVPAAMLTVSEATIYG
                     RYGFAPAARCSDFVVDTRRAGWRGPDPVAERRGAVRFVEPEVLRQVARPVFEAARRRA
                     PGQVPADDALWSEITHATLSAEDAELAPNLRAIRYEAEGETRGFALYRVEKLPEDHDV
                     TTHTATVLHLAAATDDAYAALWRFLVGLDLVAWVRASLRSVDEPLRWLIRDQRAMGIT
                     RDYDHLWVRLLDVPAALEARGYAAPGAAVVEVADPLGFAAGRFLLEVDAAGGGRVTPG
                     AAEVPAGVPVLRLGVAELGALYLGGVGAGTLARAGRVTEGAPGDAARVDALFHSPAAP
                     WLGIWF"
     gene            complement(2316..3191)
                     /locus_tag="VFQ96_02280"
     CDS             complement(2316..3191)
                     /locus_tag="VFQ96_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0256650.1"
                     /translation="MLIPARAPRLVAFDLDDTLAPSKSPVPEEMTVRLRRLLAVVPVA
                     IISGGRFAQFRQQVIERLGTVPAADLAGLHLLPACGTQYYRWAPDAEGSELVPDGPAG
                     GSWRREYSEDLNDEEKDGAIRVLEEEARRLGLWEEHPWGPIVEDRESQITFSALGQSA
                     PVDAKAAWDPDGAKRRVLRAAVAARLPGLEVRAGGSTSVDITRPGIDKAYGMRKLAGL
                     TGLALTDMLFVGDRLEPDGNDYPVKALGVPCVAVGGHRETPAVVDRILERLRDREAAA
                     TVATAASPATGRSLG"
     gene            complement(3243..4187)
                     /locus_tag="VFQ96_02285"
     CDS             complement(3243..4187)
                     /locus_tag="VFQ96_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEU0256651.1"
                     /translation="MRRVTILDDYQEVALSSADWSAVTDRFRVDVVTRRLVGDDLEAR
                     LAESEVVVAMRERTPFPAALLGRLPALRLLVTTGGRNASIDLAAARAQGVTVAGTRGA
                     GNGVAELTLGMMIALTRHFAEEDAAVRAGGWQHTIGPGLAGLTLGVLGLGRIGGPVAD
                     LARAFGMHVIAWDRSLTPERAAAHGARAVGREALFRECDILTIHLPLAEGSRGLVGER
                     EFSLLKPTAYLVNTSRGPIVDERALVAALDEGRIAGAALDVYDTEPLPAKHPLRRAPR
                     TLLLPHIGYVTTDQYARFYADAVEDIVAWDGGAPVRVL"
     gene            complement(4286..4837)
                     /locus_tag="VFQ96_02290"
     CDS             complement(4286..4837)
                     /locus_tag="VFQ96_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU0256652.1"
                     /translation="MPQEVFTIGHSTRTIEEFTALLHGSGIEVLADVRTVPRSRHNPQ
                     FAEDELRSSIPAAGIDYERMPGLGGLRYTRKADATGLNDGWRNASFRHYADYMQTGAF
                     ADALAGLVARVREHTVAIMCAEAVPWRCHRSLIGDALLVRGFAVRDIMGPTSVKPHTL
                     TRFARVDGLTITYPSAEPLPGAD"
     gene            complement(4927..5799)
                     /locus_tag="VFQ96_02295"
     CDS             complement(4927..5799)
                     /locus_tag="VFQ96_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0256653.1"
                     /translation="MKDRSVPGAETDDGRAPGAIPTLRLNDGARLPQIGLGTWPLDDD
                     EVEEAIVAAARLGYRHIDTGEPYGNEVGVGRGVRSSNLPRSEFFISTKLDGKYQGGDR
                     AIAGLEGSLERMQLDYVDLVLIHWPLPLLGEYVSTWHTFETLLARGLTKSIGVSNFAP
                     AHLAKLIAESSIVPAVNQIQVSPHVPRADYLAFHEQHGIATIAWSPLGGGTLLTDPVI
                     TKIAAEHDVSPAQVVLRWYVQQGMAAVPKTKNPARMLENRTVFDFQLTSHDMVAMKSL
                     AGGAEAVDPETDGH"
     gene            5882..6406
                     /locus_tag="VFQ96_02300"
     CDS             5882..6406
                     /locus_tag="VFQ96_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256654.1"
                     /translation="MTPQPTGRLVRREDGVYVVLDRVFLASRDDVWGSLTRPYRLAGW
                     MGECTGERETRAYRIRKRIPGMAWEDLAILECDSPSFFRGVITSDDGEERRHVYWKLA
                     RHDSYTTLTVGQRQEGRTLDPITGVRTEYLLDCLVAARAKRPLPTWEVYEAALLPFYE
                     RVVQQGAPLGTPTH"
BASE COUNT         1101 a         2396 c         2337 g          968 t
ORIGIN      
        1 agtccagacc cacgcaggcg ctcgggcagt gacagccacc gtcatgggat ggacggccga
       61 ctccatggac ccacccacgc gggactcaag gtgtgatgat cgtgcagcga acgccgggag
      121 cccggccgat gcggcggtcg agggtgacga gcggaacgcg gaggaactcg gcgagtgcca
      181 cgtaaccggc gtcatagctc gtgaggttct gccgaagctc ccaaatacgc gtggcaagca
      241 cctcgtacgg ccattgctcg atcccaaggg cgacgaggtc ggcgtgagct tgggcggcct
      301 ggtccacggt gatgaggtgc gccgccttgt gccggcggat gatgttcgcg gcctcgaatg
      361 ccaacagcgt gggcgcggcg aggctggtac ccgtcaacac cgacgccgac cactgtccgt
      421 caggccccga gtcgaggagc agcgcgacga tggcagaggc gtcgcatacg aggtgagagg
      481 tcaccggcga tccgcgtcgc gggcgacaag aatcgagcgc gcgtcgaccc gtaccccggt
      541 ggcgtcgacc cgggcccgcg cgcgggcgat cacatcgccc gccgacgggc gagacgcgct
      601 gtcgacgagc atgccgcgca catactcctg cagggattgc cccgcccgtg ccgcgcgcgc
      661 ggccagttca tcgcgcacag catccggcac atcgcgcaca gtcatcgaca cgcccatggc
      721 atcaccatag cggcaacagc tgcattgtgc atgtagaccc tgcgcgcggc caccccgcgc
      781 catgacaccc gacagacacc gacacgggcc agcacccgtc ggattcccga atcacgaagg
      841 gcgcgacgag gccggaggca tcacccatac gacaggccgg agcccctttg cgatcgccgt
      901 tgagggcggc aggaaccgac cggtgccacc gtgcaccgga gagcttcaga accagatgcc
      961 cagccacggc gcggccggcg agtggaacaa ggcatccacc cgggcggcgt cccccggcgc
     1021 accctccgtg acccgcccgg cccgggcgag ggttccagcg ccgacgccgc ccaggtagag
     1081 cgcgcccagt tcggccacgc cgagccgcag taccggcacg cccgccggca cctcggcggc
     1141 cccgggggtg acccggccgc cgcccgccgc atcgacctcc agaagaaaac ggccggcagc
     1201 gaaccccagc gggtcggcga cctcgacgac ggccgcgccg ggcgccgcat agccgcgcgc
     1261 ctccaacgcc gccggaacat cgagcagccg cacccagaga tggtcgtagt cgcgggtgat
     1321 ccccatggcg cgctgatcgc ggatcagcca ccgcaaaggc tcgtccacgc tgcgcagcga
     1381 cgccctcacc caggcgacga ggtcgagccc gacgagaaag cgccacaggg ccgcgtaggc
     1441 gtcgtcggtg gcggcggcga ggtgcagaac ggtcgccgtg tgggtagtga cgtcgtggtc
     1501 ttcggggagc ttctccacgc ggtaaagcgc gaacccgcgg gtctcgcctt cggcctcata
     1561 ccggatggcg cgcaggttcg gcgccaattc ggcgtcctcc gccgagagcg tcgcgtgcgt
     1621 gatctccgac cagagggcgt catcggcggg aacctggccg ggagcacgcc tgcgcgccgc
     1681 ctcgaacacc ggtcgagcga cctgccgcaa cacctcgggc tccacgaaac gcacggcgcc
     1741 gcgccgctcg gccaccgggt caggtccgcg ccagccggca cggcgagtgt cgaccacgaa
     1801 gtcggaacac cgcgctgccg gcgcgaaccc gtaccgcccg tagatcgtcg cctccgagac
     1861 tgtcaacatc gccgccggaa cgccggcggc gtgcgccgtg cgcaactccg cctcgagaag
     1921 cgcgcgggcg atgccgtggc gccggtgtgt agggctgacc gtgacggaac tgatggccca
     1981 cgcgtcgaca tccagcgtcg ggccgagcgg gccggggacg ctcagcggcg acacccacga
     2041 cgagacggtg gcgaccgggg tcccgggatc ggccgcgtca tcccagaccg ccgtggtgcg
     2101 gcgatacgcc gattcgggcg tgacgcgccc gacctcttct cgagtggggc gtgcgttgtg
     2161 aaaaccgcgt gcacccgcct gcagccagag cgcgaacgat gcggaggagg tgtcgacgag
     2221 cccgagccgc agcccatcgc gggcgagcct ggccttcgac tccggatcga cggggagcgc
     2281 cccggggtcg cgagggccgt gcgcggtcgc tgtcatcacc ccaggctacg gccggtcgcc
     2341 ggcgacgcgg ccgtcgccac cgtggcggcg gcctcccggt cgcgcaaccg ctccaggatc
     2401 ctgtcgacga cggcgggagt ctcgcggtgg ccgccgacgg cgacgcacgg cacaccgagg
     2461 gccttcaccg ggtagtcgtt gccgtccggc tccagccggt cgccgacgaa cagcatgtcg
     2521 gtcagggcca ggccggtgag gccggccagt ttgcgcatgc cgtaggcctt gtcgatgccg
     2581 ggccgggtga tgtcgacgga ggtggagccg cccgcgcgca cctcgaggcc cggcaagcgc
     2641 gctgcgacgg cggcccgcag cacccgccgc ttggcgccgt ccggatccca tgccgccttc
     2701 gcgtcgaccg gcgcggactg cccgagggcg gagaaggtga tctgcgactc gcgatcctcc
     2761 acgatggggc cccaggggtg ctcttcccag aggcccagcc ggcgtgcctc ctcctccaac
     2821 acccggatgg cgccgtcttt ctcctcatcg ttcaggtctt cggagtactc ccgccgccag
     2881 cttccgccgg caggcccgtc gggaaccagc tcggaaccct ccgcatccgg cgcccagcgg
     2941 taatactggg tgccgcacgc cggcaagagg tgcagcccgg cgaggtctgc ggcgggcacc
     3001 gtgccgagcc gctcgatcac ctgttgccgg aactgggcga acctgccacc cgagatgatc
     3061 gcgacgggca cgacggcgag caagcggcgc agccgaaccg tcatctcctc gggcacgggc
     3121 gacttagaag gggcgagggt gtcgtcgaga tcgaacgcca cgagtcgcgg cgcccgggcc
     3181 gggatcagca tcacggcgac aaccgtaaca tcgaagcctg accgcaccgc tggcggcccg
     3241 cgtcacagca cccgcacggg tgccccgccg tcccacgcga cgatgtcctc gacggcgtcg
     3301 gcgtaaaagc gcgcgtattg gtcggtcgtg acgtacccga tatgcggcag cagcaaggtg
     3361 cgaggcgccc gccgcagcgg atgcttcgcc ggcagcggct cggtgtcata cacgtcgagg
     3421 gccgcgccgg cgatccgccc ctcgtcaagg gccgccacga gcgcccgttc gtcgacgatc
     3481 ggcccgcgcg acgtgttgac gaggtatgcc gtgggcttca agagcgagaa ctcccgttcg
     3541 ccgaccagcc cgcggctgcc ctcggcgagc ggcaggtgga tcgtgagaat gtcgcattcg
     3601 cggaacagcg cctcccgccc gacggcccgg gcaccgtgcg ccgccgcgcg ctcgggggtc
     3661 aatgagcggt cccaggcgat gacgtgcatc ccgaaggcgc gggcgagatc ggcgacgggg
     3721 ccgccgatcc ggccgagccc aagcaccccg agcgtcagcc cggcgaggcc cgggccgatc
     3781 gtgtgctgcc agccgccggc ccgaacggcc gcgtcttctt cggcgaagtg gcgggtgagc
     3841 gcaatcatca tgccgagggt gagctcggcg acaccgttgc cggcgccgcg cgtgccggca
     3901 acggtgaccc cctgggcgcg ggcggcggcg aggtcgatcg acgcgttgcg gccaccggtc
     3961 gtgacaagca gccgcaacgc gggcagccgg ccgaggagcg ccgcgggaaa aggagtgcgc
     4021 tctcgcatcg cgacgacgac ctcgctctcg gccaggcgcg cctcgaggtc gtcaccgacg
     4081 aggcgccgcg tcacgacatc cacccggaac cggtcggtca ccgccgacca gtcggcgctg
     4141 gagagcgcga cctcttgata gtcgtcgaga atcgtcaccc gtcgcatgtt cccgctcccc
     4201 tcaccgcccg accgccgggc aacatcgttc acaccgcgcg gcgacccggg cggccccatc
     4261 acccggccgg ggtccgaggc ataccttagt cggcgccggg caacggctcg gcggagggat
     4321 acgtgatcgt caacccatcc acccgcgcga agcgggtgag ggtgtggggt ttgacggagg
     4381 tgggtcccat gatgtcgcgc acggcgaagc cgcgtacgag cagggcgtca ccgatgagcg
     4441 aacggtggca gcgccacggc accgcctccg cacacatgat ggcgacggtg tgctctcgga
     4501 cgcgtgcgac gaggccggcg agcgcgtcgg cgaacgcgcc ggtctgcatg taatcggcat
     4561 aatgccggaa cgaggcgttg cgccagccgt cgttcaggcc ggtcgcgtcg gccttgcggg
     4621 tgtagcgcag gccgccgagc ccgggcatcc gctcgtagtc gatgcctgcg gccgggatgc
     4681 tcgagcgcag ctcatcctcc gcgaactgcg ggttgtggcg ggatctcggc acggtacgca
     4741 cgtcggccag cacctcgatg ccactcccgt gcaagagcgc cgtgaactcc tcgatcgtgc
     4801 gcgtcgagtg gccgatcgtg aagacctctt gtggcatgcc cgcgcctctt ctcctcccac
     4861 ctattcaacc gcgccccgcg cggccgtgag ccgcaggcac cgcggcggcg cctgcggccg
     4921 gcagcgtcag tgaccgtcgg tctccgggtc gacggcctcg gcgccgccgg caagcgactt
     4981 catcgcgacc atgtcgtgcg aggtcagctg aaagtcgaac acggtgcggt tctctaacat
     5041 gcgggccgga ttcttggttt tcggcacggc cgccatgcct tgttgcacat accaccgcaa
     5101 gacgacctgc gcagggctca cgtcatgttc cgccgcgatc ttcgtgatga ccgggtcggt
     5161 cagtagcgtt ccgccaccca agggactcca ggcgatggtc gcgatgccgt gttgctcatg
     5221 aaaagccagg tagtctgccc tcggcacatg tgggctcacc tgaatctggt tcacggccgg
     5281 aacgatggag gattcggcga taagcttcgc gaggtgcgcg ggcgcgaagt tggagactcc
     5341 tatcgacttg gtgaggccgc gcgccagaag agtctcaaag gtgtgccagg tagagacata
     5401 ttcgcccagc aacggcagcg gccagtggat gaggacgagg tcgacatagt cgagttgcat
     5461 tctctctagc gaaccttcga ggcccgcgat cgcccggtcg cctccttgat acttgccatc
     5521 gagtttcgtg ctgatgaaga actcgctgcg cggcaggttg ctcgagcgca cgccacggcc
     5581 gacaccgacc tcgttgccat acggttcgcc ggtatcgatg tgccgatagc ccaggcgggc
     5641 cgccgcaacg atggcctctt ccacctcgtc atcgtcgagt ggccaggttc cgagcccgat
     5701 ctgtggcagg cgcgcgccgt cgttcagccg aagggtcggg atggcgcccg gggcacgccc
     5761 gtcgtcggtt tcggcgccgg gcaccgagcg atctttcaca cccgccagat taccgggatg
     5821 tcacagcttc tccccattat ttgcgggact agtgtggggc acgaacgagc gggaggtcat
     5881 catgacacca caacccacgg gccgcttggt gcggcgcgaa gacggcgtct acgtagttct
     5941 cgaccgagtg ttcctggcgt cgcgtgacga cgtctggggc agtctcaccc ggccttaccg
     6001 gttggctggc tggatgggcg aatgcaccgg ggagcgcgag acgcgggcct accgcatccg
     6061 caagcgtatt ccgggcatgg cctgggaaga cctcgcgatc ttggaatgcg attcgccgag
     6121 cttcttccgc ggcgtgatca cgagcgacga cggcgaggaa cgccgccacg tttattggaa
     6181 gctcgcgcgg cacgactcat acaccacgct gaccgtgggg cagcgtcaag agggccgcac
     6241 cctcgacccg atcacgggcg tgcgcaccga ataccttctc gactgcctcg tggcggccag
     6301 ggcaaagcgc ccgctgccca cgtgggaggt ctatgaggcg gcgctgctgc ccttctatga
     6361 gcgcgtggtt cagcagggtg caccgctcgg caccccgacg cactgacgct ctgccgcggg
     6421 cggggttcgc ctcgcagcgc tcttcacgac gacccccgcc gccgcgtaag cgctgggtag
     6481 ggcagcggcg ggccgcttca cgccgggcga cggttcttcc tccgcgtgac tctccggcac
     6541 tacaacacat agcgtctctc gcgacggccg accctgttgc gatacttctc aggcctccct
     6601 atgttgcttg ccgccttcgc tcgcgccgcc agaatccatg cccgttctta ctttttgctg
     6661 tgacgaccgc gcgacaggta attctcttcg ctactattaa cgttgcaagc gttttcgtcg
     6721 cccgaaaagg ggcgacgtca cacacaacct acggcgggag acacctgtgg catcaacagt
     6781 agagaacgag accatcaccc aa
//
LOCUS       DASTNL010000068        11712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_234143, whole genome shotgun sequence.
ACCESSION   DASTNL010000068 DASTNL010000000
VERSION     DASTNL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234143"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFQ96_02305"
     CDS             complement(<1..586)
                     /locus_tag="VFQ96_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0256655.1"
                     /translation="MSKTVRHATVVVAGLGRLGTAFALRAAVDGAAHLVVWDPDRHAL
                     AALDERLAGVAGDTTVHFQHVDLGDAGAIAAAAQRVRHRIGRVDVVITDSTTGRRAFF
                     WRSDSGEDIRRVMRVNALSPLYVAREFLPGMLAGRAPGRSIRPARILTVVPGPGPAPR
                     PRASVTAAAAAAVTTWGKSLRLELRHAGARHVGVT"
     gene            707..1723
                     /locus_tag="VFQ96_02310"
     CDS             707..1723
                     /locus_tag="VFQ96_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEU0256656.1"
                     /translation="MVQHRYLGNSGFKVSEITYGNWVTHGAQIDDDRAIATVHAALDA
                     GITTFDTADTYANTEAEKVLGRALAGQRREGLEILTKVFWPIGPKGPNDMGLSRKHIR
                     EGIDGSLRRLGVDYVDLYQAHRYDVETPLEETMQAFADVVRAGKALYIGVSEWSAEQL
                     RAAAALARELHVPLVSNQPQYSLLWRVIEEKVVPTSKELGLSQVVWSPLAQGVLTGKY
                     LPGKPAPEGSRGAGKETSKQMGERMLRDEVLTAVQRLAPIAAKAGLTMPQLAIAWVLQ
                     NPNVAAALVGATRPEQITANAAASGVTLGADVLAAIDQALGDVVVRDPSRTYAFTPKT
                     RPAQ"
     gene            complement(1810..3981)
                     /locus_tag="VFQ96_02315"
     CDS             complement(1810..3981)
                     /locus_tag="VFQ96_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0256657.1"
                     /translation="MANMQGAPASGEKQKSALEQFGVNLTEIARQGGLDPVIGRDAEI
                     RRISQVLTRRTKNNPVLIGEPGVGKTAVVEGLAQRIVAGDVAESLKDKTLIALDISAL
                     VAGAMYRGQFEERLKKVLKEVDDSNGQIITFVDELHLLMGAGGGEGSVAASNMLKPML
                     ARGELRLIGATTLDEYRQYIEKDAALERRFQQVYVGEPSVEDTIAILRGLKGRYEAHH
                     RVTITDDALVAAASLSNRYISARQLPDKAIDLIDEAMSRLKMEIDSSPVEIDELKRAV
                     DRLKIEELALKREKDDASKERLAKLQEDLAARQGELAGLEERWSRERAGLTRVGELKK
                     QLDDAVTARNKAMRDQDYAEVSRLEYTTIKELTEQLDAAQSAEGNAEGRMVGDQVTAD
                     DIATVISAWTGIPVGRLLQGESEKLLNLEGELGKRIIGQKEAVRAVSDAVRRSRAGLG
                     DADRPTGSFLFLGPTGVGKTELAKALADFLFDDAKAVVRIDMSEYGEKFAVSRLVGAP
                     PGYVGYEQGGQLTEAVRRRPYSVVLFDEVEKAHPEVFDLLLQVLDDGRLTDGQGRTVD
                     FRNTILILTSNLGAHILVDPSLTFDEKERAVLDTVRGTFKPEFVNRLDDIVVFQALTQ
                     AELGEIVSLYVDRLQARLAERRLEVGVTPDARTWLAEHGYDPVYGARPLRRLMQREID
                     DRLARRLLDGTVHDGDTVRVDLGADGRGLTVDPVDPGPEGV"
     gene            4090..5142
                     /locus_tag="VFQ96_02320"
     CDS             4090..5142
                     /locus_tag="VFQ96_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256658.1"
                     /translation="MTRQPAPTQGPVGVDFAPLESRPDWPDVKAFRRLARKAHTPGSR
                     QAPGTLPLVALAVLPLFLLAWLAFTQVGAHLGSGRTALVPPVILAAGLAVIAFLWLAA
                     LAGRSGWMRWYRIDRFATANGFQWHITSPDPGYPGTVFQQGTDRVCGETIETTTGRPL
                     RLGEYRYTLGKGKNRKIRRWGFIALRLDHRMPHMMLDGRGNNGLLGVSNLPASFARDQ
                     VLHLEGDFDRYFTLYCPRDYEKDALYLFTPDIMALFIDHAAAFDAEIVDDWLFLYAAA
                     PFDIGHPATMRRMLGVASAVGERLAQAAGRYRDDRATARAGRQAAGAGRLRPRVPWLG
                     ILVTAAAVAAILIFLL"
     gene            complement(5227..7575)
                     /gene="yicI"
                     /locus_tag="VFQ96_02325"
     CDS             complement(5227..7575)
                     /gene="yicI"
                     /locus_tag="VFQ96_02325"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF007940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEU0256659.1"
                     /translation="MKFTDGYWQLRDGVSARYARETYAVEPAEEGGVVALAVTKHIAS
                     RGDTLNTPTLTVTVTSPLPDVIGVRVEHFARHLGRGFALAKTHPPVSVEVSPEAAVVR
                     SGRLQARLGVTGPWDLTFSAEGERLTAAAERSLAYVEVAPGKTAEEIGVMGPGRLAAD
                     PALPRHYVCAQLSLGVGELVYGLGERFGPLVKNGQSVDIWNADGGTSSEQAYKNVPFY
                     LTNRGYGVFVNDSGPVSYEIGSEATERVQFSVPGESLEYFVIYGPGPKEILERYTALT
                     GRPAVVPAWSYGLWLSTSFTTRYDEETVTKFVDQMAKRRIPLSVFHFDCFWMREFAWC
                     DFEWDPRTFPDPEGMLERLHAKDLHVCVWINPYIGQRSALFAEAADAGYLLRRPDGSV
                     WQWDRWQAGMALVDFTNPAAVSWYQSKLRRLIGQGVDAFKTDFGERVPLDVRYADGSD
                     PALMHNRYTELYNSAVHEVLTQERGEAGAVLFARSATAGGQRHPVHWGGDSTSTFESM
                     AETLRGGLSLAFSGFSFWSHDIGGFEGTPDPAVFKRWLAFGLLSSHSRLHGSDSYRVP
                     WAFDAGPSAEADPQSAVSVARKFARLKNRLMPYLFAAGWQAHERGIPVLRPLQMEFPG
                     DPAVDYLDRQYMLGPDLLVAPVFDAPGRVTFYLPGGRWTSLLTGSVVEGGSWRTETHG
                     YDSLPLYLRPGALLPLGARADRPDYDYLDGLEFVVARSPRGETTSEVEILTPSGRRGV
                     FRATRSATSVRIDGDVERGWGARWAGDERAARAERGSVTLTA"
     gene            complement(7609..8424)
                     /locus_tag="VFQ96_02330"
     CDS             complement(7609..8424)
                     /locus_tag="VFQ96_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0256660.1"
                     /translation="MKRTRHTSWPSTVLAIIFLAIMIFPIYWMVNASFQSNSAAQSST
                     IFPIHPTLSGYRVALSQQGGNLVTSLIISLGTVVLSLAIATPAAYALAKFRIKGTNVI
                     MLALLITQMIPGIVIANSLYTAFNNLHLLNNYFGLILADASNCIPFSVILISAFMRSI
                     PDSLVEAARVDGAGDLRAFRSVVLPVSRNSVITAGLFSFLFTWSDFLFALTLTSTTAV
                     RPVTLGIYNYLSANVQQWAPVMATAVLASIPAMVLLLAAQKYIAAGALGGAIK"
     gene            complement(8434..9255)
                     /locus_tag="VFQ96_02335"
     CDS             complement(8434..9255)
                     /locus_tag="VFQ96_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0256661.1"
                     /translation="MVLFFGYPVVKNVTMSLQHYTASTFFTGQAPWVGFQNYVTVVKS
                     SLFTTTIVNTALFTLGSIVGQFVLGMLLALFFRRRFPLSNLLRGLLLMPWLLPLIASS
                     AVWKWILDQDSGVLNQLLGVFHLSAVPWLVSPHIALLSIVLVNVWLGIPFNVTILYSG
                     LQEIPTELYEAAELDGAGGWNMFWHITWPSLKSVVTVVLVLGVVYTLKVLDIILGLTG
                     GGPANSTQTLTTNSYQTSFTLFHFGQGAAISNILILVSLVFALIYLRFAARSNTE"
     gene            complement(9416..10678)
                     /locus_tag="VFQ96_02340"
     CDS             complement(9416..10678)
                     /locus_tag="VFQ96_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0256662.1"
                     /translation="MRGARKGALVGTVAVAALSALALTGCSSGGSSGGSGSSGSTSGG
                     AKTSIAVEDYYTQPTTGIMDKVYQACAAEENVKVTSSHVPGAGFIPKVLQQASSKTLP
                     DVLMLDNPNVKQIAQTGALSPLSDYGISAKGFAPAIAQAGSYDGKVYALAPAINTIDL
                     IYNTTMFQEAGITTPPTTWAELQTDAQKLTKKGVYGFAFSADNDGEGTWQFLPFMWSN
                     GGTETDITTAPTVQALQFLTGMIQKGTVSKSVVNWGQSDVLNQFLAGKAAMMINGPWE
                     FPQLSAQKKIVWKQSPIPTRTASQTLQSPLGGETFTVPNTGDKTKMAAAGKLVKCILS
                     PANQAKIAASEGDVPGQISVATSFAQSHPLYAAIATSVKTARARTAKLGAKWPATQTA
                     INVAEQTAFSGKASPKAALQQANSQKEQ"
     gene            10867..>11712
                     /locus_tag="VFQ96_02345"
     CDS             10867..>11712
                     /locus_tag="VFQ96_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0256663.1"
                     /translation="MKTPNAPGAAGHLERRPAATMADVARRAGVSVTTVSFVVNGTKH
                     VAPKTAARVRTAMQELHYQRNALARALASQRSHILALLFPALDRRLGRTDLTFITAAV
                     TAAAKRGYHLVIWPIGQDAGQLSELLAARLADGVLVMEVTLEDARVALLRQAGVPFTM
                     IGRTRDLTGLSYVDVDFELATSEALDHLRGLGHTRIVLVLEDLAGTPMRDYGPHVRVR
                     STYEEEMRRHGLSPQILYCAATSDGGRRAAQEILAEAPDTTAVVALGDDVANGILNEF
                     HRLGRR"
BASE COUNT         1906 a         3997 c         3940 g         1869 t
ORIGIN      
        1 gcgtcacgcc cacgtgtctg gcgcccgcgt ggcgaagttc cagccgcagc gacttgcccc
       61 acgtcgtgac cgcggcggcg gccgccgccg tcacactcgc ccggggcctc ggtgcgggcc
      121 cgggacccgg cacgacggtc aggatgcggg cgggccttat cgagcggccg ggggcacggc
      181 cggccagcat gccgggcaag aactcgcggg cgacgtagag cggcgaaagc gcgttgaccc
      241 gcatcacgcg gcggatgtcc tctcccgaat cgctcctcca gaagaacgcg cggcggccgg
      301 tcgtggagtc ggtgatgacg acgtcgaccc ggcctatccg atgccgcacg cgctgcgcgg
      361 ccgccgcgat cgcccctgcg tcgcccaggt cgacgtgttg gaagtgcaca gtcgtgtcac
      421 ctgccacgcc ggcgagcctc tcgtcgagcg cggcgagggc gtgccggtcg ggatcccaga
      481 cgacgagatg ggcggcgcca tccacggccg cccgcagggc gaatgccgtt cccagccttc
      541 cgaggcccgc gaccacgacc gtcgcatgac gcaccgtctt gctcatgcgt ccacgctaca
      601 tgcccacccc gacgccggcc gccgacgccg gctgcccgcg ccgagcaggc ccctcttcaa
      661 agcgccctct taggagcacg gccggcggtt cacgtagcct gacgctatgg ttcagcaccg
      721 gtatctcggc aacagcggtt tcaaggtctc agagatcacg tacggcaact gggtcaccca
      781 cggcgcgcag atcgacgacg atcgggcgat cgccaccgtt catgcggcgc tcgacgcggg
      841 gatcaccacg ttcgacacgg ccgacacgta cgccaacacc gaggccgaga aggtcttggg
      901 ccgggctctc gccggccagc gccgcgaggg cctggagatt ctgacgaagg tgttttggcc
      961 gatcgggccg aaggggccga acgacatggg cctgtcgcgc aaacacatcc gcgaaggcat
     1021 cgacgggtcg ctgcgccggc tcggcgtcga ctacgtcgac ctgtaccagg cccaccggta
     1081 cgacgtggaa accccgctgg aagagacgat gcaggccttc gcagacgtcg tgcgcgccgg
     1141 caaggccctc tacatcggcg tctcggagtg gtcggcagag cagctgcgcg ccgcggcggc
     1201 cctcgcccgc gaactgcacg tgccgctcgt ctcgaaccag ccgcagtatt ccctgctgtg
     1261 gcgggtcatc gaagagaaag tggtgccgac cagcaaggaa ctcggcctct cacaggtcgt
     1321 ctggtcaccg ctcgcgcagg gcgtgctgac cggcaagtac ctgccgggca agcccgcgcc
     1381 ggaaggctcc cgcggcgccg gcaaagagac cagcaagcag atgggcgagc ggatgctccg
     1441 cgacgaggtg ctcaccgccg tgcagcgcct cgcgccgatc gccgccaagg cgggattgac
     1501 gatgccacag ttggccatcg catgggtcct gcagaacccg aacgtggccg ccgccttggt
     1561 cggcgccacc cggccggagc agatcacggc caacgccgcc gcctcgggcg tcacgctcgg
     1621 ggccgacgtc ttagccgcca tcgaccaggc gctcggcgac gtggtggtgc gcgacccctc
     1681 gcgcacctac gccttcacgc cgaagacccg ccccgcccag taacagggcg aggccggcag
     1741 ggcacgagac gcggcccagg gcgccccgca cggggcagga accccgggcg gcgcagaacc
     1801 cgtcgaccct cagacgccct ccgggccagg gtcgaccgga tccacggtga ggcctcgccc
     1861 gtcggcgccg agatccacac gcaccgtgtc gccgtcgtgc accgtgccgt cgagcagacg
     1921 acgagccagc cggtcgtcga tctcccgctg catgagtcgg cgcaagggcc gcgccccgta
     1981 cacggggtcg taaccgtgct cggccagcca ggtgcgcgcg tccggcgtga cgccgacctc
     2041 cagccggcgc tcggcgagcc tcgcctgcag ccgatcgacg tagagcgaga cgatctcgcc
     2101 cagctccgcc tgggtgagcg cctggaagac gacgatgtcg tcgagtcgat tgacgaactc
     2161 cggcttgaag gtgccccgca cggtgtcaag cacggcccgc tctttctcgt cgaacgtcag
     2221 cgacgggtcg acgagaatgt gggccccgag gttcgaggtg aggatgagga tcgtgttgcg
     2281 aaagtcgacg gtgcggccct gcccgtcggt gaggcggccg tcgtcgagaa cctgcaggag
     2341 caggtcgaag acctcggggt gcgccttctc cacctcgtcg aaaagcacga cggagtatgg
     2401 cctgcgccgc accgcctctg tcagctggcc gccctgttcg tagccgacgt accctggggg
     2461 cgcgccgacg aggcgcgaga ccgcgaactt ctcgccatac tcgctcatgt cgatacgcac
     2521 gacggccttg gcgtcgtcaa agagaaagtc ggccagagcc ttcgcgagct ccgtcttgcc
     2581 gacgcccgtg gggccgagga agaggaacga gccggtcggt cggtcggcgt cgccgagccc
     2641 ggcacgggag cgccgcaccg cgtcggagac ggcccgcacc gcctctttct ggccgatgat
     2701 ccgcttgcca agctcgccct ccaggttgag gagcttctcg ctctcgccct gcaggaggcg
     2761 gcccaccgga atgccggtcc acgcggagat gaccgtggcg atgtcgtcgg cggtcacctg
     2821 gtcgccgacc atgcgcccct ccgcgttccc ctcggcgctc tgggccgcat cgagctgctc
     2881 ggtgagctct ttgatcgtgg tgtattccag gcgggagacc tcggcgtagt cctggtcacg
     2941 catcgccttg ttgcgcgcgg tgacggcgtc atcgagctgc tttttgagct cgccgacccg
     3001 ggtgaggccg gcccgctccc ggctccagcg ctcctccagc cccgcgagct cgccttggcg
     3061 ggcggccagg tcttcttgca gcttggcaag acgctccttc gaggcgtcgt ctttctcccg
     3121 cttcagcgcc agctcttcga ttttcagccg gtcgacggcg cgcttcagct cgtcgatctc
     3181 gacgggtgac gagtcgatct ccatcttgag ccggctcatc gcctcgtcga tcaggtcgat
     3241 cgccttgtcg ggcagttggc gggccgagat atagcggttc gagagggagg cggcggcgac
     3301 gagcgcgtcg tcggtgatcg tgacccggtg gtgcgcctcg taacggccct tcaacccccg
     3361 caagatcgcg atggtgtctt cgacgctcgg ctcgccgacg tacacctgct ggaagcgccg
     3421 ttcgagcgcc gcatccttct cgatgtactg ccggtactcg tcgagggtcg tcgcgccgat
     3481 cagccgcaac tcgccgcggg ccaacatggg cttcaacatg ttggaggcgg cgacggagcc
     3541 ctcgccgccg cccgcgccca tcagcaggtg cagttcatcg acgaacgtga tgatctgtcc
     3601 gttcgagtcg tcgacctctt tcaagacctt cttcagccgt tcttcgaact ggccgcgata
     3661 catggctccg gcgacgaggg cggagatgtc aagcgcgatg agcgtcttgt ctttcagcga
     3721 ctcggcgacg tctcccgcga cgatgcgttg ggcgaggccc tcgacgacgg cggtcttgcc
     3781 gacgcccggc tcgccgatga gcaccgggtt gttcttcgtg cgccgggtga gcacctggct
     3841 gatccggcga atctccgcgt cgcggccgat cacggggtcg agcccgccct gccgggcgat
     3901 ctccgtgaga ttgacgccga actgttcgag cgcgctcttc tgcttctccc cgctcgcggg
     3961 agccccctgc atgttcgcca tggatctcct tgtctggtct tgtgccggcg ccgatcacca
     4021 acttgagtct aattggctca actttattgc aaggcgggtt attccgccag ggccgatagc
     4081 ctagacggcg tgacccgcca gcccgcaccg acccagggcc cggtcggggt ggatttcgcg
     4141 ccgctggaaa gccggccaga ctggcccgac gtgaaggcct tccgccgcct ggcacgcaag
     4201 gcccacacgc ccggctcccg ccaggctccc ggcaccctgc cgctcgtcgc gctcgcggtg
     4261 ctgccgctct tcctcctggc gtggctggcg ttcacgcagg tcggcgcgca cctgggcagc
     4321 ggccgcacgg ccctcgtgcc gccggtgatc ctcgccgcgg gcctcgccgt gatcgccttt
     4381 ctgtggttgg cggcgctcgc aggccgtagc ggctggatgc ggtggtaccg catcgaccgc
     4441 ttcgccacgg ccaacgggtt ccaatggcac atcacctcgc ccgaccccgg ataccccggc
     4501 accgtgttcc aacagggcac cgaccgcgtg tgcggcgaga cgatcgagac gacgaccggc
     4561 cgccccctgc gcctggggga ataccggtac acgctcggca agggcaagaa ccgcaagatc
     4621 cgacgctggg gattcatcgc gctgcgcctc gaccacagaa tgccgcatat gatgctcgac
     4681 ggccgcggca acaacgggct cctgggcgtc agcaacctgc ccgcctcctt cgcccgagac
     4741 caggtgctgc acctggaggg cgactttgac cgctatttca cgttgtactg cccccgcgac
     4801 tacgaaaagg acgcgctcta cctcttcacg cccgacatca tggcgttgtt catcgaccac
     4861 gcggcggcgt tcgacgccga gatcgtagac gactggctgt tcctttacgc ggccgctccg
     4921 ttcgacatcg gccatccggc gaccatgcga cgcatgctcg gcgtcgcctc ggccgtgggc
     4981 gaacgcctgg cccaggcggc cggccgctac cgcgacgacc gcgcgacggc cagggccggc
     5041 aggcaggcgg caggtgccgg ccggctgcgg ccccgggtcc cctggcttgg catcctcgtg
     5101 accgcggccg ccgtcgcggc gatcctgatc ttcctgctct gaggcacgcg gaagccgttc
     5161 cgcagcgccc ggcgcgtgcg ggtcggcaag gaacgagaac gggggaggag gagcgagaga
     5221 ccggggtcag gcggtgagcg tgacgctgcc gcgctccgcc cgggcggccc gctcgtctcc
     5281 ggcccaccgg gcaccccacc cgcgctcgac gtcgccgtcg atgcggacgg atgtcgccga
     5341 acgggtcgca cgaaagacgc cccgccggcc agacggcgtc aaaatctcga cctccgacgt
     5401 cgtctcgccc cgcggcgaac gcgcgacgac gaactccagc ccgtcgagat agtcgtagtc
     5461 aggccggtcg gcccgggcgc cgagcggcag gagggccccg gggcgcaggt agagcggcag
     5521 cgagtcatag ccgtgggtct ccgtgcgcca tgacccgcct tccaccacgg aaccggtcag
     5581 aaggctcgtc caccggccgc ccggcaggta gaacgtcacc cggccgggag cgtcgaagac
     5641 cggcgcgacg agaaggtcgg ggccgagcat gtactgccgg tcgaggtagt cgacggcggg
     5701 atcgccggga aactccatct gcaggggccg cagcacgggg atgccccgct cgtgcgcctg
     5761 ccagcctgcg gcgaagaggt agggcatgag ccggttcttc aaccgcgcga acttgcgcgc
     5821 cacgctgacg gcgctctgcg ggtcggcctc cgccgaaggc cccgcgtcga acgcccacgg
     5881 cacccgatag gagtcgctgc cgtgcaggcg cgaatggctg gagagaaggc cgaaggcgag
     5941 ccagcgtttg aagaccgccg ggtcgggcgt gccctcgaag cctccgatgt cgtggctcca
     6001 gaacgagaac ccgctgaacg ccagagagag cccgccgcgc agcgtctctg ccatcgattc
     6061 gaacgtcgag gtgctgtcgc cgccccagtg aacggggtga cgctggccac cggccgtcgc
     6121 cgagcgggcg aagagcaccg ctccggcctc gccgcgctcc tgggtcagca cctcgtgaac
     6181 ggcgctgttg tacagctccg tgtagcggtt gtgcatcagg gccgggtctg acccgtctgc
     6241 gtagcgcacg tcgagcggca cacgctcgcc gaagtctgtc ttgaaggcgt cgacgccttg
     6301 gccgatgagc cttcgcagct tcgactggta ccacgagacc gccgcagggt tggtgaagtc
     6361 gaccaacgcc atgccggcct gccacctgtc ccactgccac acgctgccgt cgggccggcg
     6421 caagagatag ccggcgtcgg cggcctcggc gaagagcgcc gagcgctgcc cgatatacgg
     6481 gttgatccag acgcagacgt gcagatcctt cgcgtgcagc cgctccagca tcccctccgg
     6541 gtcggggaag gtgcggggat cccactcgaa gtcgcaccag gcgaactccc gcatccagaa
     6601 gcagtcgaaa tggaagacgc tgagcgggat gcgccgcttc gccatctggt cgacgaactt
     6661 ggtgacggtc tcctcgtcgt accgggtcgt gaagctcgtc gagagccata aaccgtacga
     6721 ccaggcgggg acgacggctg gccggccggt caaggccgtg tagcgttcga ggatctcttt
     6781 cgggcccggc ccgtagatga cgaagtattc gaggctctcg ccgggcaccg agaattgcac
     6841 ccgttccgtg gcctccgagc cgatctcgta cgagacgggg cccgaatcgt tgacgaagac
     6901 gccatacccg cggttcgtca ggtagaacgg cacgttctta taggcctgct cgctggaggt
     6961 gccgccgtcg gcgttccaga tgtcgacgct ctgcccgttt ttcaccaggg ggccgaagcg
     7021 ctcccccagg ccgtagacca gctcgccgac gcccagggac agctgcgcgc acacataatg
     7081 ccgcggcagg gcggggtctg cggccagccg gcccgggccc atgacgccga tctcctccgc
     7141 ggtcttgccg ggcgcgacct cgacgtaggc cagcgaacgt tccgcggcgg ccgtcagcct
     7201 ctcgccttcg gcgctgaacg tcagatccca gggccccgtc acgccgagcc gcgcctgcag
     7261 ccggcccgac ctgaccacgg ccgcctccgg cgacacctct acggacaccg gagggtgggt
     7321 cttcgcgagc gcgaacccgc ggccgaggtg ccgcgcaaaa tgctcgaccc gcacgccgat
     7381 cacgtcgggg agcggagagg tgacggtcac ggtcagcgtc ggagtgttga gggtgtcgcc
     7441 tctggacgcg atgtgtttcg tcaccgcgag ggctacgaca ccgccctctt ccgcaggctc
     7501 gacggcgtac gtctcgcggg cgtaacgtgc cgacacgccg tcgcgtagct gccaatagcc
     7561 gtcggtgaac ttcatgtgtg cctttcgctt gccggcaagg gtggtcggct atttgatcgc
     7621 gccgccgagc gccccggcgg cgatgtattt ctgggcggcc agcagcaaga ccatggcggg
     7681 gatcgacgcc aagaccgcgg tcgccatgac cggcgcccac tgttggacgt tcgcgctcag
     7741 atagttgtag atgcccaacg tcacggggcg caccgccgtt gtgctcgtca gcgtcagcgc
     7801 gaaaaggaaa tcgctccacg tgaacaaaaa gctgaagagc ccggcagtga tgacggagtt
     7861 gcgactgacc ggcaagacca cggaacggaa cgcccgcaga tccccggcgc cgtcgacacg
     7921 tgccgcctcg acgagggagt ctgggatcga acgcatgaac gcgctgatca agatcacgga
     7981 gaacggaatg cagttggagg catccgcgag gatcaagccg aagtagttgt tgagcaggtg
     8041 caggttgttg aaggccgtgt agagcgagtt ggcgatgacg atgcccggga tcatctgggt
     8101 gatgaggagc gccaacatga tcacgttggt gcccttgatg cggaacttcg ccagtgcgta
     8161 ggccgcgggg gtcgcgatcg cgagcgacaa gacgacggtg cccagcgaga tgatgaggct
     8221 cgtgacgagg ttgccgcctt gctgcgacaa cgcgacgcga tagcccgaca gcgtcgggtg
     8281 gatcgggaag atcgtgctgc tctgcgcggc gctgttgctt tggaagctcg cgttgaccat
     8341 ccagtagatc ggaaagatca tgatcgcgag aaaaatgatg gccaagacgg tcgacggcca
     8401 gctcgtgtgt cgggttctct tcattctcgg cgcctactcc gtgttcgagc gcgcggcgaa
     8461 gcgcagatag atcagtgcga agacgaggga gaccaggatg aggatgttgc tgatcgcggc
     8521 gccctggccg aaatggaaga gcgtgaacga ggtctggtag gagttcgtcg tcaacgtctg
     8581 cgtcgagttc gccggacccc cgcccgtcag cccgaggatg atgtcgagca ccttcaacgt
     8641 gtagacgacc ccgagcacga gcaccacggt gacgaccgac ttaaggctcg gccaggtgat
     8701 gtgccagaac atgttccacc ctccggcgcc gtcgagctcg gccgcttcgt acagctccgt
     8761 gggaatctcc tgcagcccgc tgtacaagat ggtcacgttg aacggaatgc cgagccatac
     8821 gttgacgagg acgatcgaga gaagtgcgat atgcgggctc accagccagg gcacggcgga
     8881 gaggtggaag acgccgagca gctggttcag cacgccactg tcttggtcga ggatccactt
     8941 ccagaccgcg ctcgaggcga tcaggggcag caaccacggc atgagcaata ggccccgcag
     9001 caggttggag agcgggaagc gccgccggaa gaagagcgcc agcagcatgc caagcacgaa
     9061 ctggccgacg atcgagccga gggtgaagag ggcggtgttg acgatcgtcg tcgtgaacag
     9121 gctgctcttc acgacggtga cgtagttctg gaaaccgacc cacggggcct gtcctgtgaa
     9181 gaaagtgctc gccgtgtagt gctgcaggct catcgtcacg ttcttgacga ccgggtagcc
     9241 gaagaacaag accatatagg cgatggccgg tgccaagaag agccagcgcg gcccctcccg
     9301 ggtgagccac gacggcctgc gccgtggctc acccggaact gccgcggcgg ctgccccgcg
     9361 ggtggcgggg tggtcgaccg aggtcgttga cattatcgtt cgcgccagcc gatctctact
     9421 gttctttttg gctgttggcc tgctggagcg ccgccttggg agacgccttg cccgagaagg
     9481 ccgtctgctc cgccacgttg atggcggtct gcgtcgcggg ccacttggcg ccgagcttgg
     9541 cggtgcgggc gcgcgcggtc ttgaccgagg tcgctatggc cgcatacagc ggatgcgact
     9601 gggcgaagct cgtcgccacg gagatctgcc cgggcacatc gccctccgac gccgcgatct
     9661 tcgcctggtt ggcagggctg aggatgcact tcacgagctt gcccgcggcg gccatcttcg
     9721 tcttgtcgcc ggtgttcggc acggtgaagg tctcgccgcc cagcggcgac tgcaacgtct
     9781 gcgacgcggt gcgcgtcggg atgggagact gcttccagac gattttcttc tgcgcagaga
     9841 gctgcgggaa ctcccacggc ccgttgatca tcatggcggc cttgccggcg aggaactggt
     9901 tgagcacatc gctctgcccc cagttcacga cgctcttcga gacggtgccc ttctggatca
     9961 tgcccgtcaa gaactgcagc gcctgcaccg tcggcgcggt cgtgatgtcg gtctccgtgc
    10021 cgccgttcga ccacatgaac ggcaggaact gccaggtgcc ttcgccgtcg ttgtcggcgc
    10081 tgaacgcgaa cccgtacacg cccttcttcg tcagcttctg cgcgtcggtc tgaagctctg
    10141 cccacgtcgt aggcggcgtc gtgatgcccg cctcttggaa catcgtggtg ttgtagatga
    10201 ggtcgatcgt gttgatggcc ggggccaggg cgtagacctt gccgtcgtag ctgcccgcct
    10261 gggcgatggc aggagcgaag cccttcgccg agatcccgta gtcgctaagc ggcgacaacg
    10321 cccccgtctg cgcgatctgc ttgacgttcg ggttgtcgag catcagcacg tcgggaagag
    10381 tcttcgacga cgcctgctgc agaaccttgg gtatgaagcc cgccccggga acgtgcgatg
    10441 aggtaacctt cacgttctcc tcagccgcgc aggcctggta gaccttgtcc atgatcccgg
    10501 tggtcggctg ggtgtagtag tcctcgacgg cgatcgacgt cttcgcgccg cccgacgtgc
    10561 tgccgctcga acccgacccg ccgctcgacc cgccgctcga gcagccggtg agcgcgagag
    10621 cggacaaagc cgccaccgcg acggtgccga cgagggcacc ttttcttgca ccacgcatcc
    10681 tgaagcccct ttgcagtctg tgacctgtgt tcgcgccgat cgaggcgacc cgagaacggg
    10741 agatctcgtg ggcacaaagc gattaaagcg ctttactaac tcgctgtcaa accgctttac
    10801 tatgctgtcc accaggggct gtcgccctgc ccagccgaca caaggaatcg atccgccgtg
    10861 agcgccatga agacaccgaa cgcccccggg gcagccgggc acctcgaacg acgccccgcc
    10921 gcgacgatgg ccgacgtcgc acgccgggcc ggggtgtcgg tgacgaccgt ctccttcgtg
    10981 gtcaacggaa ccaagcacgt cgcgccgaag acggccgccc gggtgcgcac cgcgatgcag
    11041 gagctgcatt accagcgcaa cgccctcgcc cgggcgctgg cgagccagcg ctcgcatatc
    11101 ttggcgttgc tcttcccggc gctggatcgc cgcctcggcc gcacggacct gacgttcatc
    11161 acggccgccg tcaccgccgc cgcgaagcgc ggctaccacc tcgtcatctg gccgatcgga
    11221 caagacgccg gccagctctc cgagctgctc gccgccaggc tcgccgacgg ggtcctcgtc
    11281 atggaggtca cgttggaaga cgcgcgcgtc gcactcctac gccaggcggg ggtgccgttc
    11341 acgatgatcg gccgcacccg cgacctcact gggctctcct atgtcgatgt cgacttcgag
    11401 ctggccacct cggaggcgct cgaccacttg cgagggctgg gccacacccg catcgtgctc
    11461 gtgctggaag acctcgccgg cacgccgatg cgagactacg gccctcacgt ccgagtgcga
    11521 tcgacatacg aggaggagat gaggagacac ggcctcagcc cgcaaatttt atattgcgct
    11581 gcgacctcgg atggcgggcg tagggcggca caggagatct tggccgaggc acccgacacg
    11641 acggcggtcg tcgccctcgg tgacgatgtc gccaacggca tcctcaacga gttccaccga
    11701 ttaggccgcc gt
//
LOCUS       DASTNL010000069         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_604739, whole genome shotgun sequence.
ACCESSION   DASTNL010000069 DASTNL010000000
VERSION     DASTNL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604739"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFQ96_02350"
     CDS             <1..233
                     /locus_tag="VFQ96_02350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEU0256664.1"
                     /translation="KLATVVVAVITSNTSLAALPGNVFLPASAIGLPKDSVVNVTAIV
                     TLNKTDLTDLVGAVPTGLVLDVDRGLRQVMGL"
     gene            288..1094
                     /locus_tag="VFQ96_02355"
     CDS             288..1094
                     /locus_tag="VFQ96_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0256665.1"
                     /translation="MSTLAERFLALHTPGKPLVIPNPWDVASARILAKIGFRALATTS
                     SGYAASVGRRDGQVSRAEALDYAARIVRATDLPVSADLERGFGAGPKEVAETYRLAAR
                     IGLAGASIEDASGEADRPLYDIPRAADRVRAAAEAAHGTVQGLVLTARAENFFHGSTD
                     VSDVVARLQAYQEAGADVLYAPGPTDLADIRAIVSSVDRPVNVLLRPGGPGVAELAEA
                     GVARVSVGGGPTFAVYGAFATAARELFEHGTAGYGEAARAGHEATASFGA"
     gene            1164..2015
                     /locus_tag="VFQ96_02360"
     CDS             1164..2015
                     /locus_tag="VFQ96_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0256666.1"
                     /translation="MSVPGAIVRFAETRESLDKLNDLASGWVKRLTRRRAVKNLLSGT
                     GLGHPLHPLLTDVPIGAWTMASMLDLTAGRRGADAARRLVGVGVLGAVPTIASGAADW
                     SDTYGPEQRLGHVHAIANIVGTLLQTGSWLARRRGRRGTGVLLSLTGIGVTAAAGYLG
                     GALSFGRGVGVNHTAFEEAPEGWVDVAADADLQQDRPLRVDAAGTPVMLVRHRERIRA
                     LSATCVHAGGPLDEGEIVGDAVRCPWHGSEFRLADGQPTRGPAAISQPVWNVKVEAGR
                     VLVRPRA"
     gene            complement(2027..>3706)
                     /locus_tag="VFQ96_02365"
     CDS             complement(2027..>3706)
                     /locus_tag="VFQ96_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU0256667.1"
                     /translation="PARRPHSGQASVARFLQLRRIARSHHLTPLRRLDFSRAPESSAL
                     RVRQAEGMRHALEEAGGAFVKMGQMLSTRDDLLPAEWLRALSQLQKNVKPAPWPAVRR
                     MLENEWETGLKDIFAEFDETPLASASIAQVHKARLRDGSVVAVKVRRPGIVPLIRRDV
                     HIALRMAGLLARTNAQARRLGIEQVARQYTGDLLRQTDFRLEAANLVALTEAQERGRA
                     DDQVRLPRLYAGLSTSSVLVMEFLPGETLSAVVDGRHGRAAGREHRDDLTEVLRTVVR
                     TFLDELIFDGVYHADLHPGNIMIGPDDSPAFIDFGSVGRLSRDLRQTVQDLLIGYLAG
                     DTRRIADAILTMSSLPDGADEREFRSDVSSFVTYRLGPGARISISTVDAAVEMLAKHR
                     LALPPEFVAAARAFAVLEATARNLQADFDTLQEAKRLTGRVVSGRMTRAGIRQFTAAG
                     LSELLPGLRRLPRRLDRITAALEEGRLNVNLRLLADRRDRRLLMSFVRQAVLTVIGIV
                     AGVQGVLTLAAAPPAHPGLIRTATAGLILCTVAALAFVGVGFDMWRTRRRG"
BASE COUNT          523 a         1335 c         1319 g          529 t
ORIGIN      
        1 gcaagcttgc gacagttgtc gtcgcggtga tcacctctaa cacctccctc gccgccttgc
       61 ctggcaacgt gttcctgccg gcctccgcga tcgggctgcc gaaagactct gtggtcaacg
      121 tcaccgcgat cgtcacgctg aacaaaaccg acctgacgga tctcgtcggc gcggtgccga
      181 cgggcctcgt gcttgacgtc gaccgcggcc tacggcaggt gatgggcctc tgatcccgcg
      241 atccggagcg tgacgcgtag caccacgcgg cggcctaggg tgaaggcgtg agtacactcg
      301 ccgaacgttt tctcgccctg cacacccccg gaaagccgct cgtcatcccc aacccgtggg
      361 acgtcgcctc cgcccgcatc ttggccaaga tcgggtttcg ggccctcgcc acgacgagtt
      421 ccggttacgc cgcctcggtc ggccgccgcg acgggcaggt ctctcgcgcg gaggcgctag
      481 actacgccgc ccgcatcgtg cgggcgaccg atctgccggt cagcgccgac ctggagcgcg
      541 ggttcggcgc cgggccgaaa gaagtggccg agacataccg cctggcggcc cgcatcgggc
      601 tcgccggcgc ctcgatcgaa gacgccagcg gcgaggcaga ccgcccgctc tacgacattc
      661 cgcgggccgc cgaccgggta cgcgccgcgg cagaggcggc gcacggcacc gtgcaaggcc
      721 tcgttctcac cgcgcgtgcc gagaacttct tccacgggtc gaccgacgtc tccgatgtcg
      781 tcgcccgcct gcaggcgtac caagaggccg gcgccgacgt gctctacgcc cccggcccga
      841 ccgacctcgc cgacattcgc gccatcgtgt cctccgtcga ccggccggtg aacgtgctgc
      901 tgcgccccgg agggcccggc gtcgccgaac tcgccgaggc gggcgtggca cgcgtctcgg
      961 tcggcggtgg ccccacgttc gcggtctatg gcgcgttcgc gacggcagcc cgcgagctct
     1021 tcgagcacgg caccgcagga tacggcgagg cggcacgcgc cggccacgag gcgaccgcct
     1081 cgttcggcgc ctgagccgcg ccgcacggcg ggcccggccg catgccatag tgagaacgtc
     1141 ggtgtgatct gcaagggggc aatatgtcgg tgccgggcgc catcgttcgt ttcgcggaga
     1201 ccagggaatc gctagacaag ctgaacgacc ttgcctcggg ctgggtgaaa agactcaccc
     1261 gccgtcgcgc cgtcaagaac ctgttgtcag gcacgggcct ggggcacccg ctgcacccgt
     1321 tgctcaccga cgtgccgatc ggcgcgtgga cgatggccag catgctcgac ctcacggccg
     1381 ggcggcgcgg ggcggatgcg gcgcgcaggc tcgtcggcgt gggcgttctc ggggccgtgc
     1441 cgacgatcgc ctccggcgcc gccgactggt ctgacaccta cggcccggaa cagcggctcg
     1501 gccacgtgca cgccatcgcc aacatcgtcg gcacgcttct gcagaccggc tcctggctgg
     1561 cccgtcggcg cggccggcgc ggcaccggcg tgctcctctc cctcaccggc atcggcgtca
     1621 cggcggcggc cggctacctc ggcggcgcac tctccttcgg ccgcggtgtc ggcgtgaacc
     1681 acacggcgtt cgaagaagcc cccgaaggat gggtcgacgt cgccgccgac gccgacctgc
     1741 agcaagatcg cccgctgcgc gtcgacgcgg cgggcacgcc cgtcatgctc gtgcgccaca
     1801 gggagcgtat tcgcgccctc tcggcgacct gcgtgcacgc cggcgggccg ctcgacgaag
     1861 gcgagattgt gggcgacgcc gtgcgctgcc cgtggcacgg cagcgagttc cgtctcgccg
     1921 acggccagcc gacccgcggg ccggccgcga tcagccagcc ggtctggaac gtgaaggtcg
     1981 aggccggccg tgttctcgtg cggccccgcg cctgaccggc ggccgatcag ccgcggcgcc
     2041 gggtgcgcca catatcgaag ccgacgccga cgaaggcgag agcggccacg gtgcacaaga
     2101 tgagtcccgc cgtcgcggtg cggatgaggc ccgggtgggc gggcggcgcg gcggccagcg
     2161 tcaagacgcc ctgcacgccg gcgacgatgc cgatcaccgt caacaccgcc tgcctgacga
     2221 acgacatgag cagccgccgg tcgcgccggt cggccagcag gcgcaggttg acgttcaggc
     2281 ggccttcttc cagtgccgcc gtgatccggt cgaggcggcg cggcaggcgc cgcagccccg
     2341 gcagcagctc ggaaagcccc gccgcggtga actgccgtat gccggcccgg gtcatccgcc
     2401 cgctcaccac ccgcccggtg agtctcttcg cctcttgcag cgtgtcgaag tcggcctgca
     2461 ggttgcgtgc ggtcgcctcc agcaccgcga acgcgcgcgc cgccgcgacg aactcgggcg
     2521 gcagagccag gcgatgcttg gcgagcatct cgacggcggc gtcgacggtc gagatcgaga
     2581 tgcgagcgcc tgggcccagc cggtaggtca cgaacgagct cacgtcggag cggaactctc
     2641 gctcgtcggc accgtcgggc aggctcgaca tggtgaggat cgcgtcggcg atgcgccgcg
     2701 tgtcgccggc gaggtagccg atcaacaggt cttgcacggt ctggcgcagg tctcgcgaaa
     2761 gccggcccac ggagccgaag tcgatgaagg cggggctgtc gtccggcccg atcatgatgt
     2821 tgccggggtg cagatcggcg tggtagacgc cgtcgaagat caactcgtcg aggaacgtgc
     2881 gtaccacggt gcgcagcacc tcggtcagat cgtcgcggtg ttcccggccg gcggcccggc
     2941 cgtgcctgcc gtcgaccacg gcactcaggg tctcgccggg caggaactcc atgaccagca
     3001 ccgaacttgt cgacaggccc gcatacagcc ggggcagccg cacctggtcg tcggcgcggc
     3061 cgcgctcctg cgcctcggtc agggcgacga ggttcgccgc ttcgagacga aagtcggtct
     3121 gccgaaggag gtcacccgtg tattggcgcg ccacctgctc gatgccgagt cgccgggcct
     3181 gcgcgttcgt ccgcgccaga aggcccgcca tgcgcagcgc gatgtgcacg tcgcggcgaa
     3241 tgagcggcac gatgccgggc cggcgcacct tgacggccac cacgctgccg tcgcgcagtc
     3301 tcgccttgtg cacctgggcg atggaggcgg aggcgagggg cgtctcgtcg aactcggcga
     3361 agatgtcctt cagccccgtc tcccactcgt tctcgagcat gcgccgcacg gccggccagg
     3421 gggccggctt cacgttcttc tgtaactggg agagtgcgcg gagccactcg gcgggcagca
     3481 ggtcgtcgcg ggtggagagc atctggccca tcttcacgaa cgcgccgccg gcctcttcga
     3541 gggcgtgccg catgccctcc gcttgccgta cgcgcagcgc cgacgactcc ggggcgcggc
     3601 tgaagtcgag tcggcgcagg ggcgtcaggt ggtggctgcg tgcgatgcgg cgcaactgca
     3661 gaaaccgggc gacgctcgct tgcccgctgt gtggcctgcg ggcggg
//
LOCUS       DASTNL010000070         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_237168, whole genome shotgun sequence.
ACCESSION   DASTNL010000070 DASTNL010000000
VERSION     DASTNL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237168"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFQ96_02370"
     CDS             <1..271
                     /locus_tag="VFQ96_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256668.1"
                     /translation="ARAIAAGPKVLVCDEPTSSLDVLVQARVLDLLDEMQRRLGLAYL
                     FISHDLGVIRHVSDEVAVMEHGRIVAQGPADAVLPGTGVRAVRWA"
     gene            347..817
                     /locus_tag="VFQ96_02375"
     CDS             347..817
                     /locus_tag="VFQ96_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0256669.1"
                     /translation="MAGKPIVPPFSEEDAAAKVRAAEDLWNTRDPERVAAAYAPESHW
                     RNRTTWVNGRDEIVAFLTQKWERERDYRLIKELWSFGGNRIAVRFASESMGKDGLWYR
                     SYGNEQWQFDEEGRCLFRHASINDLQIAEADRLYRWEAPGPRPAGHPGLTELGL"
     gene            complement(879..2432)
                     /locus_tag="VFQ96_02380"
     CDS             complement(879..2432)
                     /locus_tag="VFQ96_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEU0256670.1"
                     /translation="MGTEQNDNTPSPVAQAPQPPRAGLTRRSLVIGGGLAGVVALGVG
                     AGIGVAVSRDDAKKPTPAPSAKPSDGLRHFHGTNLAMPAVAGWRVSDTQKGHLFVTQQ
                     TDTMGGKGFRGTIMDDRGEPVWIEPTGANVTGLTVQTFEGKPVLTYWSGTSIGGHGEG
                     VGRILDTSYEPVATVHAGDGLKADLHEFHLTTSGTALVTAYQTIPADLTSIGGPKKGY
                     IYNCHAQEVDIRSGKVLFDWNVNDHVPVSDTYLGLKQDKGHDGTSASRAFDAYHINAI
                     DYWDDMLLVSLRHTHTVYAVSKADGSIRWRLGGKHSYFTVQPDAAFAWQHDARRQPDG
                     TITLFDNHLYSGTNGQSHGKRFRLDETARTATLLTDLTFNRHLGTAMGSVQPMADGHT
                     LVGWGVAPFVTEFDANGEVVYQADLGAMSYRAFRHDWTGAPKTKPEISASTSADTTAV
                     HMSWNGATEIAHWRILAGETGSSLHEVATVPHSHFETLARIAATKAVRAQALDKSGAV
                     LGTTRAIKA"
BASE COUNT          461 a         1117 c         1130 g          514 t
ORIGIN      
        1 cgcccgggcg atcgccgccg gccccaaggt gctggtctgc gacgagccga cctcgtccct
       61 cgacgttctc gtgcaggccc gggtgctcga cctgctcgac gagatgcagc gccggctcgg
      121 cctggcctac ctgttcatct cccacgacct cggcgtcatc cggcacgtca gcgacgaagt
      181 cgcggtcatg gagcacggcc ggatcgttgc ccaaggcccg gccgacgccg tgctgcccgg
      241 caccggagtg cgggccgtac gctgggcttg agccgtctcg gcccgaaccg gtcgccaaga
      301 ccacggccgc gggtcgccca agaagctcac caaggagatg caggccatgg caggcaaacc
      361 gatcgttccc ccgttcagcg aggaagacgc cgccgcgaag gttcgcgcgg cggaagacct
      421 gtggaacacg cgcgacccgg agcgggtcgc cgccgcgtac gcacccgaga gtcactggcg
      481 caaccgcacg acctgggtca acggacgcga cgagatcgtg gcgttcttga ctcaaaaatg
      541 ggagcgcgag cgcgactacc ggctgatcaa ggaactgtgg tcgttcggcg gcaaccgcat
      601 cgccgtgcga ttcgcctcgg agtcgatggg caaagacggc ctctggtacc gctcgtacgg
      661 caacgagcag tggcagttcg atgaggaggg gcgctgcctc ttccgccacg ccagcatcaa
      721 cgacctgcag atcgccgaag ccgaccgcct ctaccggtgg gaggcgccgg gcccgcgccc
      781 cgccggccac ccgggcttga ccgagctcgg tctgtaggct ctctcgcccg gcccgcgcgc
      841 gggcgtcgcc gacgcggcgc gtccttgggc gggccgggtt atgccttgat cgcccgggtg
      901 gtgccgagca ccgcgccgct cttgtcgagc gcctgcgcgc ggaccgcctt cgtcgccgcg
      961 atgcgggcaa gggtctcaaa gtggctgtgg ggcacggtcg ccacctcgtg aagcgaggag
     1021 cccgtctcgc ccgccagaat ccgccaatgc gcgatctcgg tggcgccgtt ccagctcatg
     1081 tgcaccgccg tggtgtcggc gctcgtgctg gccgagatct ccggtttcgt cttcggagcc
     1141 cccgtccaat cgtggcggaa ggcgcggtac gacatcgcgc cgagatccgc ctggtagacg
     1201 acctcgccgt tcgcgtcgaa ctcggtgacg aacggggcga cgccccagcc gaccagcgtg
     1261 tgaccgtcgg ccatcggctg cacgctgccc atcgcggtgc cgagatgccg attgaaggtg
     1321 aggtcggtga ggagggtcgc cgtgcgcgcc gtctcgtcga ggcggaaccg tttgccgtgc
     1381 gactgcccgt tcgtgcctga gtagaggtgg ttgtcgaaga gggttatcgt gccgtccggc
     1441 tgccgcctgg cgtcgtgctg ccacgcgaag gcggcatccg gctggactgt gaagtagctg
     1501 tgcttgccgc ccagccgcca ccgaatcgac ccgtccgcct tgctgaccgc atacacggtg
     1561 tgagtgtgcc gcaacgacac gagcagcatg tcgtcccagt agtcgatggc attgatgtgg
     1621 tacgcatcga acgcccggct cgccgacgtg ccatcatgcc ccttgtcttg cttcaaaccg
     1681 agataggtgt cggagacggg aacgtggtcg ttcacgttcc agtcgaagag caccttgccg
     1741 gagcggatgt cgacctcttg cgcgtggcag ttgtagatgt accccttttt cggcccgcct
     1801 atcgaggtga gatcggccgg tatcgtctgg taggcggtca caagggcggt gcccgaggtc
     1861 gtcaggtgga actcgtgcag gtcggccttc aggccgtcgc cagcgtggac cgtcgcgacc
     1921 ggctcgtacg aggtgtcgag gatgcgcccg acgccctcac cgtgaccccc gatgctggtc
     1981 cccgaccaat aggtgagcac gggcttgccc tcgaacgtct gcacggtgag gcccgtcacg
     2041 ttcgcgccgg tcggttcgat ccacacgggc tcgccgcggt cgtccatgat cgtgccgcgg
     2101 aatcccttgc cgcccatcgt gtcggtctgc tgcgtgacga agaggtgtcc cttctgagta
     2161 tcgctcaccc gccagcccgc gaccgcaggc atggcgaggt tcgtgccgtg gaagtgacgc
     2221 aacccgtcag acggtttcgc ggagggcgcc ggcgtgggct tcttcgcgtc gtcgcgactg
     2281 acagcgacgc cgatgccggc gccgacaccc agcgcgacga ccccggccag cccgccgccg
     2341 atgacgagcg agcgccgcgt gaggccggca cgcggcggct gcggcgcctg cgctacgggg
     2401 gagggagtgt tgtcgttctg ctcggttccc ataatgccgc cagagtagta agacgacatt
     2461 tgcgtctgct atgtccgcta catggccttc cttcgagtgg ggccacgggc ggggcccgcc
     2521 cggcgagtga cgcccggggc ctcgacccgc ttggcgcgct gccggccggc acccttgggg
     2581 tccggcggcg ccccgtgtgg acacgggcgc ggcacacttg gcgcactgcc agccgacacc
     2641 tctgggggcc cggcgacacc ccgtgtggac acgggcgtcc aggcggtctc ttctgtggcg
     2701 cccccggggt ccggcgacac cctacgggcc ggcgcccagg gcacggtcgg cgcagatgcc
     2761 gaccctgggg ccgacccagg cccctgcagg ccgacgaagt cttcgtccgg tcgcggttta
     2821 cctgtgcttg gctgcccgaa gcgctgctgg ggtcggcgcc cccgtgccac gacccactgc
     2881 acgcaacctc ccgcgggccg ggcgacaccc tggggtccga cgacgcccta ccgaggcgac
     2941 gcccccgcgc cgagacccgc tgcacacgac cttccgtagc ctcccggggg tcaggttcga
     3001 cactccgaca gcacggcacg ggaatacgtc acggcccggc ccgacttctt gtgagaggaa
     3061 cacccgcgcg cctcatggcc gccgggcgtg gcgcgggcgg cgtgcgggaa aggattatgg
     3121 cgtggatgca ggacggctgg cattgcgggt cgtggtcggc gggctcttga tcgggcacgg
     3181 cacccagaag ctcttcggct ggttcggcgg cgggggccag gc
//
LOCUS       DASTNL010000071         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_793212, whole genome shotgun sequence.
ACCESSION   DASTNL010000071 DASTNL010000000
VERSION     DASTNL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793212"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1485
                     /locus_tag="VFQ96_02385"
     CDS             235..1485
                     /locus_tag="VFQ96_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0256671.1"
                     /translation="MARTTAPDFVSAPEADYEQIAGVFRPIFARIAEGASAREQDHRL
                     PYEEIKQLTDAKFGALRVPVEYGGFGASLPQLFRLLTELAAADSNIPQALRGHFAFVE
                     DRLVARDGRRDAWLRRFARGEIAGNSWTEVGTVALGDVITKVSPIPGGDGSEFHVNGT
                     KYYSTGSIFADWIDTFAQRTDDGRKVIAVIDAHQEGVTHGDDWDGFGQQTTGSGTSTF
                     VDAVVKSEDVIDFDTRFKYQTAFYQLVLLTVLAGSITAAEREVAAEVRARTRIFSHGN
                     ADSFAADPQILQVVGEVSAQAYAAEATVERAAQALQEAFEGAFLDDEAEDERRNDVAE
                     LESAKAQVVLTDLATRATSNIFNALAASGVSTHKNFDRHWRNARTAANHNPWVYKARI
                     VGDHSVNGAIPPRVWSIGAGPKKS"
     gene            complement(1555..>2071)
                     /locus_tag="VFQ96_02390"
     CDS             complement(1555..>2071)
                     /locus_tag="VFQ96_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0256672.1"
                     /translation="TGRGKREAMRPYMDKVVPEAWQAAEEYSAAVRHAAAAKGLEAQE
                     AELISLRASQMNACAFCLDVHARRARAAGLTQQKLDMLPAWRESDLFTVREAAVLSIA
                     EAATRLPPTEEGEADLEAARGVLGDETFAAAEWIAVTINTFNRISVLSRHPVRPRDAD
                     GAVIGRGATRR"
BASE COUNT          360 a          717 c          675 g          319 t
ORIGIN      
        1 ggcacgcgcc gagcggcaaa cataccggcc gtcgtcgcag gaggagacgc cgtcgactcg
       61 ctgcccacct gacgcactgc ccacccccgg catatgtcgt ctgccacaga cggcctgccc
      121 ggcctcattc gccgcaccgc atctgccgca cctcctctgt cccacctcgt ccgtccgatg
      181 ccgtctgtca caacacggca tgcacccaac ctcttcatca cgaaaggcat tcccatggca
      241 cgtacaactg cacccgattt cgtctcggcc ccagaagccg actacgagca gatcgcaggc
      301 gtcttccgcc cgatcttcgc gcgtatcgct gagggggcgt ccgccagaga acaagaccac
      361 cggctgccct acgaggagat caagcagctc acggacgcca agttcggtgc gttgcgggta
      421 cccgtcgaat atggcgggtt cggcgccagc ctgccgcagc tgttccggct gctcaccgag
      481 ttggcggcgg ccgactcgaa catcccgcag gcgttgcgcg ggcatttcgc gttcgtcgaa
      541 gaccggctcg tggcccgcga cggccggcgt gacgcctggc tgaggcgatt cgccagagga
      601 gagatcgccg gcaactcgtg gacggaggtc ggcacggtgg ccctcggcga cgtgatcacc
      661 aaggtgagtc ccatccccgg aggcgacgga agtgagttcc acgtcaacgg aacgaagtac
      721 tactccacgg gcagcatctt cgcggattgg atcgacacgt tcgcgcagcg cacagacgac
      781 ggccggaagg tgatcgcggt gatcgatgcg caccaggagg gcgtgacgca cggggacgac
      841 tgggacggct tcggacagca gaccaccggt tcggggacca gcacgttcgt ggacgccgtg
      901 gtgaagtcgg aggatgtgat cgacttcgac acgcgcttca aatatcagac tgcgttctac
      961 cagctggtcc tcttgaccgt ccttgccggc agcatcaccg cagccgaacg ggaagtcgcc
     1021 gccgaagtac gtgcccgcac ccgcatcttc tcccacggca acgcggactc gttcgcggcc
     1081 gacccgcaga tcttgcaagt ggtcggcgaa gtctcggctc aggcttatgc ggcggaagcg
     1141 acggtcgagc gtgcggcgca ggcgttgcag gaggcgttcg agggcgcctt cctcgatgac
     1201 gaggccgagg acgagcgtcg aaacgacgtc gccgaactcg agtcggcaaa ggcgcaagtt
     1261 gtcctgaccg acctggcgac ccgggcgaca tcgaacattt tcaacgcact ggccgcatcc
     1321 ggcgtcagca cgcacaagaa tttcgaccgg cactggcgta acgcccgcac ggccgccaac
     1381 cacaatccat gggtgtataa ggcgcggatc gtcggcgacc actcagtcaa cggcgcgata
     1441 ccgccacggg tctggtcgat cggcgccggc cccaagaagt cctagggtcc gccggccttc
     1501 ccgtggcccc gtgcatgggg tggccgacac agccggcccg acctgggcgg cacctcagcg
     1561 acgtgtggct ccccggccga tcacggcccc gtcggcgtcg cgtgggcgca ccggatgccg
     1621 gctgaggact gagatgcggt tgaaggtgtt gatggtcacg gcgatccatt cggcggcggc
     1681 gaaggtctcg tcgccgagca cgccgcgggc cgcctccaga tccgcctcgc cttcctcggt
     1741 gggcggcagg cgcgtggcgg cctcggcaat ggagagcacc gcggcctccc ggacggtgaa
     1801 gagatcggat tcacgccaag ccgggagcat atcgagcttc tgttgcgtga ggcccgcggc
     1861 ccgggcacgg cgcgcatgca cgtcgaggca gaaagcgcag gcgttcatct gcgaggcgcg
     1921 cagcgagatc agctccgcct cctgcgcctc gagacccttc gcggcggccg cgtgacgcac
     1981 agccgctgaa tactcctcgg cggcctgcca cgcctccggc acgaccttgt ccatatacgg
     2041 ccgcattgcc tctcgcttcc cccggcccgt g
//
LOCUS       DASTNL010000072        21268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_794847, whole genome shotgun sequence.
ACCESSION   DASTNL010000072 DASTNL010000000
VERSION     DASTNL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 21268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21268
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794847"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFQ96_02395"
     CDS             complement(<1..547)
                     /locus_tag="VFQ96_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0256673.1"
                     /translation="MRPRSLDEVVGQQGLLRDGSPLARLAQAAPETRGAVSVILWGPP
                     GTGKTTLAGAIAGTSGRNFVELSAVTAGVRDVRAVMDKALGDRDLYGISTMLFLDEIH
                     RFSKAQQDALLPGVENGWVVLVAATTENPSFSVIAPLASRSLVLRLEPLSDEDLGVVL
                     DRAVTDPRGFGGRISVDPDARA"
     gene            689..1915
                     /locus_tag="VFQ96_02400"
     CDS             689..1915
                     /locus_tag="VFQ96_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEU0256674.1"
                     /translation="MATSDQHPWGRVDETGTVFVRTADGERAVGQFPDGSADEALAYF
                     ERKFADLEGQVRLLEQRARLGSAANAVAKAAATLRANLDDAAAVGDLAALVARVDALV
                     PMIQNLGERQDAEAKEALRAAIDERTLVVEEMERLAAQDPATIRWKDASARMDELFAQ
                     WRAQQAATRLPKATAEGLWKRFRQARSGLERGRRAFFAQLDETHRAAKRRKEELVGEA
                     EALIAAGSSGTRTYQGLLTAWKAAGRAGKKTDDALWARFKAAGDAIYAAKGAEDAEAH
                     QEQEENLAAKRALLDEAEPALKGADRAEARQRLRSLQEHWDEIGPVPRESLRAVEGRL
                     RKMEQAVRKMDDDAWREHDPEKKARSDGMVAQLTDSIAQLERDLAAASAAGDARRSAD
                     IAEALETRRHWLDALR"
     gene            2017..2574
                     /locus_tag="VFQ96_02405"
     CDS             2017..2574
                     /locus_tag="VFQ96_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256675.1"
                     /translation="MSRLPRVLRETDLPAPELGAARLDGEVFALDAGRYACVDECADP
                     ALRAACLASLIPAAAIVEGGSASWVYGARARPPARHTLCIEVTRRARIMPDVRWKVRE
                     RRLGDADTVDLAGIRILAPLRLACDLARGAVWETPQQTEFREVLRAGHVSLNTVRESL
                     QQGRALPGTKAAVARLQSPPARALA"
     gene            complement(2588..4840)
                     /locus_tag="VFQ96_02410"
     CDS             complement(2588..4840)
                     /locus_tag="VFQ96_02410"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0256676.1"
                     /translation="MVDTRTQQSTTLRRLVPRLFSRSQHTQAVERLIRTVRTHHPKAD
                     TALIERAYTTAEHAHAGQKRYSGEPYIIHPVAVAQILAELGTGAKTVTAALLHDTVED
                     TDYSLDALRRDFGDEVAMLVDGVTKLDKVTYGESAQAETVRKMIVAMSKDIRVLIVKL
                     ADRLHNARTWGFVPVESAQRKAKETLEIYAPLAHRLGIQTIKLELEDLSFAVLQPKIY
                     EEIDSLVKRRSPQRKRVVGEVIQIVKRELKEARIRGEVSGRPKEYYSIYQKMVVRGRE
                     FDDIYDLVGIRVIVGSVRDCYAVLGQVHSLWTPMPGRFKDYIATPKFNLYQSLHTTVI
                     GPEGRPVEIQIRTEEMHRRAEYGVAAHWKYKEQASGRGVATTANASGADLAWLAHISD
                     WQAETADPGEFLDSLRFEIGAKEVYVFTPKGRVIGLPAGSTPVDFAYAVHTEVGHRTM
                     GARVNGRLVPLESSLNTGDVVEVFTSKNPDAAPSRDWLNFVKSPRARTKIRQWFTKER
                     RDEAIEAGKDAIARAMRKQNLPVQKLMGQDVLGEVAADLHYTGVSPLYAAVGEGHVST
                     QSIIEKVLATAQDDAEREDDDQLRPVGQRRGVRHSDSGVLVRGAPDILVKLAKCCAPV
                     PGDAIIGFVTHGSGVSVHQAGCVNVAMLRKQPDRIIEVEWAPGSHSLFLVQIQVEALD
                     RSGLLSDVTRVLSEYHVNILSATVHTSKDRLALSRFAFEMGDTTHLDRVLNAVRRIDG
                     VYDVYRVSSN"
     gene            complement(4930..5952)
                     /gene="secF"
                     /locus_tag="VFQ96_02415"
     CDS             complement(4930..5952)
                     /gene="secF"
                     /locus_tag="VFQ96_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU0256677.1"
                     /translation="MPTRFQRFGNDLYTGALSIDFVGHRRRKIWYSIAAVLIIASVLI
                     PIFHRGFNFGIDFVGGSQFSITQVKTTDQGLARSAVASVDPSITATVTTINGNAVQVQ
                     TEQLTSQKSLEVGQALATAYKVPVADVTSTFIGPSWGADVSKKAIQGVVVFLLLAFIA
                     MALYFRTWKMSAAAILSLLHDMIITAGVYAAVGFEITPATMIGFLTILAYSLYDTVVV
                     FDQINENTVDDATKLKRTFAESVNLAVNQTLVRSINTGVIAVLPVAAILFIGTLVFGA
                     DTLRDISLSLFIGIILGTYSTIFIASPLYAEFREGEDKVAKHDKRVLALRAKAASSEQ
                     PELVAE"
     gene            complement(5952..7682)
                     /gene="secD"
                     /locus_tag="VFQ96_02420"
     CDS             complement(5952..7682)
                     /gene="secD"
                     /locus_tag="VFQ96_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU0256678.1"
                     /translation="MAKSTPVKSAWRALIWLGAIVVALGVVLAGGVQFSHAGWAPKLG
                     LDLEGGTEIILAPQLEHGAKITSDELNQAVSIIRQRIDASGVSEAQINVQSGNNIDVT
                     IPGRHLDNSTRQRIEASSKMELRPVLVAGAATAPVPSPSATAGAKALSNTPKTKPKNG
                     SDPAWVTPALQQKFTDLDCKTAENKLTSVQDPNKPLVTCDDAGQNKYILGPVEVDGSS
                     ISDASAGLVTSQSGVTTGEWAVNVTFSGQGKTNFATVSKRLYALEQANQNDPRARFAF
                     VIDGKVLEAPSMNGIITNGQAQITGGFTETSAKNLANELKFGALPVSFRVQSNNTISS
                     TLGSSQLKSGLLAGLIGLGLVVLYTLFQYRLLGLVTITSLLGSAVITYLVIVLLSWRI
                     DYRLSLAGVAGLIVAIGFTADSFIVYFERIRDELREGRGLASAVEAGWKRARHTIYAS
                     KATNLLAAIVLYVLAVGDVQGFAFTLGVTTVIDVIIVLLFTHPTLRLLARTNYFATGH
                     KMSGLDPEAHGVAVYRGAGHFRTHVTERRESKLASSGREAAKRQTIAERKAAEREAAK
                     VGQGTGGKES"
     gene            complement(7754..8143)
                     /locus_tag="VFQ96_02425"
     CDS             complement(7754..8143)
                     /locus_tag="VFQ96_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU0256679.1"
                     /translation="MDPIMYVMLAVLAVMVFFMFRNGKKRQREQAELRSKVVTGADVM
                     TNFGVFGRVIDIDEEKNEVLLETTPGTILRVHRQVVSRVVTPDEEPADDAAGEAREGS
                     FDDARPADAEPEFGERVDGERPSADNK"
     gene            complement(8205..9245)
                     /gene="ruvB"
                     /locus_tag="VFQ96_02430"
     CDS             complement(8205..9245)
                     /gene="ruvB"
                     /locus_tag="VFQ96_02430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0256680.1"
                     /translation="MNGTVVDPSPEGDAELAFEGALRPHSLAEFVGQEKVKGQLRLLL
                     EAAALQGRTADHILLAGPPGLGKTTLAMIVAAEGGHALRISSGPAIQHAGDLAAVLSS
                     LTPGEVLFIDEIHRMARSAEEMLYLAMEDFRIDIMVGKGAGATSIPLDLAPFTLVGAT
                     TRSGLLPNPLRDRFGFTAHLEFYAPKELAEVVARTAAKLGVAIDGRAVAEIAGRCRGT
                     PRIANRLLRRVRDYALVHGTPVGLDTVRRALELYDVDEMGLDRLDREVMTGILDRFDG
                     GPVGLSTLAVSVGEEPETIESVVEPFLVRTGLIARTPRGRVATRAAWQHFGRVPPAER
                     AASQGLPGIGDL"
     gene            complement(9242..9865)
                     /gene="ruvA"
                     /locus_tag="VFQ96_02435"
     CDS             complement(9242..9865)
                     /gene="ruvA"
                     /locus_tag="VFQ96_02435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0256681.1"
                     /translation="MISSLRGTAVAVTPTAVELEVGGVGYTVAVTPRHASRLRVGAEA
                     LVYTRLVMRADAVSLYGFVGVDERAVFDLLTGVNGVGPKSAMGVLTELSPAQIGEAVA
                     REDDAAFRAVSGIGTKTAKLIVVQLTGKMEHFRTPSAGEAAPAAGGVDAAVVEALVGL
                     GWPERVARETVEEARGSEGADADVPTLLRLALRRMVPGARPAERVAR"
     gene            complement(9862..10458)
                     /gene="ruvC"
                     /locus_tag="VFQ96_02440"
     CDS             complement(9862..10458)
                     /gene="ruvC"
                     /locus_tag="VFQ96_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU0256682.1"
                     /translation="MTRRVVGIDPGLTRCGVGVIDVGPTRSAALVYVGVVRSAAQARL
                     GARLVAIADGVERLIDEYQPQAFAIEEVFAQHNVRTVIGVAQISGILVAAATRRGLPV
                     GIHTPTQVKSAVTGYGRAGKAQVQAMVARILGLAAPPSPPDAADALAIALCDAWGGGT
                     GPAGTGEQGAAGSELATPALRRWREAERKAGAGRRLGR"
     gene            complement(10467..11231)
                     /locus_tag="VFQ96_02445"
     CDS             complement(10467..11231)
                     /locus_tag="VFQ96_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0256683.1"
                     /translation="MSGHSKWATTKHKKAVIDQRRAKSFAKLIKNIEVAAKIGGADLS
                     GNPTLVDAVQKAKKTSVPNENIDRAIKRGAGLTGESVEYTTIMYEGYGAGGVAFLIEC
                     LTDNKNRAAADVRTAMTRNGGTMADPGSVAYNFTRKGVIVVPEADGVDEDAVLAAVLE
                     AGAEDVTGHAAGFEVVTEPTDMVAARTALQEAGIDYDSADVEFVANVSIDADTETARK
                     VLRLIDALEDSDDVQNVFTNIELSAAVQAELDMEDA"
     gene            complement(11342..11989)
                     /gene="pdxT"
                     /locus_tag="VFQ96_02450"
     CDS             complement(11342..11989)
                     /gene="pdxT"
                     /locus_tag="VFQ96_02450"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU0256684.1"
                     /translation="MTPAPAAPGEAAQPRVGVLALQGDFREHLAVLKALGADAIPVRR
                     PHELASVYGLVIPGGESSVMDKLSRQFGLAEPLREAIRDGMPIYGTCAGLIMLSETIL
                     DGIAGQESLGGLDICVRRNAFGSQVDSFEVDLDVPRLEGDRPVHAVFIRAPIVESLGP
                     GARPLACLADGRVVAVEQGNLIGTSFHPEMTGEDRFHRYFLAKVAEYRDHVLARV"
     gene            complement(11997..12914)
                     /gene="pdxS"
                     /locus_tag="VFQ96_02455"
     CDS             complement(11997..12914)
                     /gene="pdxS"
                     /locus_tag="VFQ96_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU0256685.1"
                     /translation="MVAQENAVTGSETKFGSNRVKRGLAEMLKGGVIMDVVTREQARI
                     AEDAGAVAVMALERVPADIRAQGGVARMSDPDLIEGIIDEVSIPVMAKARIGHFVEAQ
                     VLQALNVDYVDESEVLSPADYVNHIDKWRFTVPFVCGATNLGEALRRITEGAAMIRSK
                     GEAGTGDVSEATKHMRKISAQIKALTAMSDDELYVAAKELQAPYGLVQEVASLGKLPV
                     VLFTAGGIATPADAAMMMQLGADGVFVGSGIFKSGDPAKRAAAIVKATTFYEDASVIA
                     EVSRGLGEAMVGINVSDIPAPHHLADRGW"
     gene            complement(12991..13533)
                     /locus_tag="VFQ96_02460"
     CDS             complement(12991..13533)
                     /locus_tag="VFQ96_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU0256686.1"
                     /translation="MDVVDPGRLTGVPDEFQRLWTPHRMAYIAEGADHTAHGCPFCAA
                     PSRSDEAALIVYRGKSAYALLNLYPYNSGHLLVCPYRHVALYDEASAEEVAEIGEITQ
                     TAMRVIRQVSANDGFNIGMNQGRVAGAGISEHLHQHIVPRWAHDANFLPIIAKTKALP
                     QLLGEMRDAIAAAWPKTAAE"
     gene            complement(13593..15560)
                     /gene="thrS"
                     /locus_tag="VFQ96_02465"
     CDS             complement(13593..15560)
                     /gene="thrS"
                     /locus_tag="VFQ96_02465"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0256687.1"
                     /translation="MADGFELFTDRTVVAMRVNGILKDLAATVTPQDTVEPVTITSPD
                     GLAILRHSAAHVLAQAVQSVNPEVKLGIGPPIADGFYYDFDVAAPFTPDDLKALRKAM
                     ERIVRQGQRFVRRVVTEDEARAELADQPYKIELIGLKAHVANATDNESVEVGAGELTM
                     YDNVDPATGETVWKDLCRGPHLPNTRMIGRAFDLMRVAAAYWRGSEKNPQLQRIYGTA
                     WASKDDLKAYQARLEEAAKRDHRKLGAELDLFSFPDEIGSGLAVFHPKGGIVRAEIEG
                     YLRTQLLRNGYELVSTPHITKGHLFEISQHLNWYKEGMFPPMHLDEERDEAGNITRQG
                     QDYYLKPMNCPMHNLIFRARGRSYRELPLRLAEFGTVYRYEKSGTLAGLTRVRGLTQD
                     DAHVYVTEDQVKDEVAGQLRFMLATLRGYGLDDFYLELSTKDPEKYVGSDAVWEAATE
                     TLREVALGSGLELVPDPGGAAFYGPKISVQARDAIGRTWQLSTVQLDFNQPELFDLQY
                     TAPDGSRKRPVMIHRALLGSIERFFAILLEHYAGAFPVWLAPTQAVGIPIADHHRDYL
                     AGILGRLRERDVRAVLDDSDDRMQKKIRTHTRQRVPYLLIAGDEDQAAGTVSFRFRDG
                     SQRNGVPVDEAIRRITQTIDAHEQVVTAWPA"
     gene            complement(15635..16054)
                     /locus_tag="VFQ96_02470"
     CDS             complement(15635..16054)
                     /locus_tag="VFQ96_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU0256688.1"
                     /translation="MAEDPREWNRSIIEEFRSGGGTTRIFGRNLVLLHHRGAKTGTER
                     VTPLIARHPDPSTWLLSASANGAPHNPAWYHNLVAHPDDVQIETPDDGTVPVRAEDLS
                     GPAREKAWAQFVSFSDRYTGYQEKTTRTIPVLALHRQ"
     gene            complement(16094..16165)
                     /locus_tag="VFQ96_02475"
     tRNA            complement(16094..16165)
                     /locus_tag="VFQ96_02475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16131..16133),aa:Val,seq:gac)
     gene            complement(16190..16260)
                     /locus_tag="VFQ96_02480"
     tRNA            complement(16190..16260)
                     /locus_tag="VFQ96_02480"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16226..16228),aa:Cys,seq:gca)
     gene            complement(16310..16381)
                     /locus_tag="VFQ96_02485"
     tRNA            complement(16310..16381)
                     /locus_tag="VFQ96_02485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16347..16349),aa:Gly,seq:gcc)
     gene            16703..16777
                     /locus_tag="VFQ96_02490"
     tRNA            16703..16777
                     /locus_tag="VFQ96_02490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16735..16737,aa:Val,seq:cac)
     gene            complement(17047..17991)
                     /locus_tag="VFQ96_02495"
     CDS             complement(17047..17991)
                     /locus_tag="VFQ96_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0256689.1"
                     /translation="MSETSSVTRPPCTTGAAHDLLVCDVEPCDEAYPPGFQVWVDYFQ
                     ANPERQRRLERAWDPAAPVVLTTRQRRALVRSFQRFELGEGGDGVHLLAKARAAGDPA
                     YTRALELLVVEEQKHSALFGVGLRRMKAPSLRGHWSDVAFTRLRRLLGLRTELALFLI
                     AEAVAIEYFEALADSAPDPVLRGIGERIRTDEREHLRFQVDRLRVGFADASRVTKAIA
                     GAAWWVVAVGAASVLCVDHAAALRICGRRPVAYWGRALRRFRGLARSALSIPRDLGTA
                     SAGRSRVFLLGPGVRVAGDGGHDGPSVGRAARATGVAL"
     gene            18053..18535
                     /locus_tag="VFQ96_02500"
     CDS             18053..18535
                     /locus_tag="VFQ96_02500"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEU0256690.1"
                     /translation="MSQVIIVFIRVFIGLIALGTLAAQVLVIPEFAAELGTVSDMPSA
                     SVPYAVAGIIVGACFEVALTATWMLVSMVSRDAIFTENAFRWVDAIIGASLTALALVV
                     AFGAHAVFVLQPRLDSPSIELVVGATVICGATFVLLVIVLRGLLRNATNLRSELAEVI
                     "
     gene            18535..18747
                     /locus_tag="VFQ96_02505"
     CDS             18535..18747
                     /locus_tag="VFQ96_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0256691.1"
                     /translation="MAIIVDLDVMLARRKMSVGDFASAIGITPANVAVLKNGRAKAVR
                     FTTLDAICRVLQCQPGDLLRWQPDDE"
     gene            complement(19149..19502)
                     /locus_tag="VFQ96_02510"
     CDS             complement(19149..19502)
                     /locus_tag="VFQ96_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256692.1"
                     /translation="MRLYRGTVVSASDPSVDIVFADETHDLVILRLRGGTREEMLGGR
                     HAILRSTASRPDYVVTDDRGVTVAVRESIDYSANLPGTLEVIVPSPSRLNLAGILTLT
                     SRSDPITITFSLQPL"
     gene            complement(19867..20478)
                     /locus_tag="VFQ96_02515"
     CDS             complement(19867..20478)
                     /locus_tag="VFQ96_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEU0256693.1"
                     /translation="MIETYGRWPRVAAAALCPAWAAALANRRRLARDGGWVAGLALPI
                     LLAHQTEEWVRPGGFLPFANKRLLGSRRPDWPLTRRVGFHVNVTAGWGSAVAGMLLWK
                     RSPGVAAGVVAMEAGNVAMHAGMAVRQRRYNPGLATAVVLMAPHAVASARWLARSGRM
                     SRGGTAMAALIGAAFAALPVSMKLRMRDGRHLRPPSPATASEI"
     gene            complement(20609..21031)
                     /locus_tag="VFQ96_02520"
     CDS             complement(20609..21031)
                     /locus_tag="VFQ96_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0256694.1"
                     /translation="MSVTGRTDVSDRDYAKLLRLRTRLREFEYWSAQQAAARGLTSAQ
                     HQLMLAVRGHKGDKPPTIGQIAGYLMIGHAAAVGLADRTEALGLVCRHRDRDDHRIVR
                     LTLTAVGAEKLAELTGAHLAELDHIEPLLAELYGDLNS"
BASE COUNT         3501 a         7164 c         7101 g         3502 t
ORIGIN      
        1 cggcacgggc atccggatcc acactgatgc ggccgccgaa cccgcggggg tccgtcacgg
       61 cccggtcgag cacgacgccc aagtcctcgt cactgagggg ctcgagtctc aagacgagcg
      121 agcgggaggc cagcggtgcg atgacggaga acgacgggtt ctccgtcgtc gccgccacca
      181 ggacgaccca gccgttctcc accccgggca agagagcatc ttgttgtgcc ttggagaagc
      241 ggtggatctc gtcgaggaag agcatcgtgg agattccgta aagatcccgg tcacccagtg
      301 ctttgtccat gacggcccgc acgtcgcgca cccccgccgt gacggccgaa agctcgacga
      361 aattgcggcc ggacgtaccg gcgatagccc cggcgagggt ggtcttgcct gttccgggcg
      421 gtccccagag gatgacggag acggcaccgc gggtctcggg tgcggcctgg gcgaggcgcg
      481 cgagcgggga gccgtcacgg agcaggcctt gctggccaac cacttcgtcg agcgatcgtg
      541 gccgcatgcg cacagcgaga ggggtacccg acgctctgcg ttccggcgtg ctcaccaccc
      601 caggctaacg gcgtgggcct ctggtggcgt gcaataggct ggcaccggca cgcggcgcat
      661 ctgccgcctg aggacaaggt gacatccatt ggctacgagc gatcagcatc cctggggccg
      721 ggtcgacgag accggcaccg tcttcgtgcg caccgccgac ggtgagcgcg cggtcgggca
      781 gttccccgac ggcagcgccg acgaggcgct ggcctacttc gagcgtaagt tcgccgatct
      841 ggaggggcag gtgcgtctgc tcgaacaacg cgcccgactc ggaagcgccg cgaacgccgt
      901 cgcgaaggcc gccgccacgc tccgggcgaa cctagacgac gcggcggcgg tcggcgacct
      961 cgccgcactt gtcgctcgcg tcgacgcgct cgttcccatg atccaaaacc tgggcgaacg
     1021 ccaagacgcc gaggcgaaag aggccctgcg cgcagcgatt gacgagcgca cgctcgtcgt
     1081 cgaagagatg gaacggctgg ccgcccaaga cccggcgacg atccgatgga aagacgcgtc
     1141 ggcccgtatg gacgagctct tcgcccagtg gcgggcgcag caggccgcga cgcgcctgcc
     1201 gaaggcgacg gcggaagggc tttggaagcg cttccgccag gcgaggtccg gcctggaacg
     1261 cggcaggcgg gcgtttttcg cgcagctcga cgagacacac cgggcggcga agcggcgtaa
     1321 ggaagagctc gtgggcgagg ccgaggcgct catcgctgct gggtcgtccg gcaccaggac
     1381 gtaccagggc ctcttgaccg catggaaggc cgcgggtcgc gccggcaaga agacggatga
     1441 cgccctctgg gcccgcttca aggcggccgg cgacgcgatc tacgcggcga agggcgccga
     1501 agacgccgag gcgcatcagg aacaagagga gaaccttgcg gcgaaacgcg ccctcctcga
     1561 cgaggccgag ccggcgctga aaggcgccga ccgggccgag gcacggcaaa ggctgcgttc
     1621 gctgcaagag cactgggacg agatcgggcc ggtgccacgc gagagcctgc gtgccgtgga
     1681 gggccggctg cgcaagatgg agcaggccgt ccgcaagatg gacgacgacg cgtggcggga
     1741 gcacgacccc gagaagaagg cccgttccga cggcatggtg gcgcagctga ccgattcgat
     1801 agcccagctg gaacgcgacc tggccgcggc ctccgcggcc ggagacgccc gccgcagcgc
     1861 agacatcgcc gaggccttgg agacgcgtcg acactggctc gacgcgctgc gctgagtcgg
     1921 cgcgcgaggt atccacagcc ggtgccgccc ggcccggatc tccacaatcg cagcttcggc
     1981 gcatcgggcg gcgagtgccg gccggatgct tagtgcatga gccgactccc ccgtgtcttg
     2041 cgtgagaccg acctgccggc acccgagctg ggcgctgcac gcctcgacgg cgaggtcttc
     2101 gcgcttgacg ccgggcgtta cgcctgcgtc gacgaatgcg ccgaccccgc cctccgggcg
     2161 gcctgtctgg caagcctcat tcccgccgcc gcgatcgtcg aaggcggcag cgcctcgtgg
     2221 gtgtacggcg cccgtgcccg ccctcccgcg cggcacacgc tgtgcatcga agtcacacgg
     2281 cgggcccgca tcatgcccga cgttcggtgg aaggttcggg agcggcggtt gggcgacgcc
     2341 gacaccgtcg accttgccgg catccgcatc ctggcgccgc tgcgcctcgc ctgcgacctc
     2401 gcgcgaggag cggtatggga gacgccgcag cagaccgagt ttcgagaggt tctgcgggcc
     2461 ggccacgtga gtctcaacac ggtacgcgag agcctgcagc agggccgggc gctgcccggc
     2521 acgaaggccg ccgtggcacg cctgcagtca ccgcccgcgc gggccttggc atgaggagag
     2581 gccgacccta gttggaagag acgcgataga cgtcgtagac gccgtcgatg cggcgcaccg
     2641 cgttcagcac acggtcaagg tgcgtcgtgt cgcccatctc gaacgcgaac cggctgagcg
     2701 cgaggcggtc cttcgaggtg tgcaccgtcg ccgaaaggat gttcacgtgg tattcggaga
     2761 gcacccgggt cacgtcggag agcagccccg accggtcgag cgcctccacc tgtatctgca
     2821 cgaggaacag gctgtgcgag ccgggtgccc actccacttc gataatgcgg tctggttgtt
     2881 tgcgcagcat cgccacgttg acacaaccgg cctgatgcac cgaaaccccg gaaccgtgcg
     2941 tgacgaaacc gatgatcgcg tcgcccggca ccggggcgca gcatttggcc agcttgacga
     3001 gaatgtcggg agcaccgcga acgagcacac cggaatcgct gtgtcgcacg ccgcgccgct
     3061 ggccgacagg cctcagctgg tcgtcgtctt cgcgttccgc gtcgtcttgc gcggtcgcca
     3121 gcaccttctc gatgatcgac tgcgtcgaga cgtggccctc gccgacggcc gcgtacaagg
     3181 gcgagacgcc tgtgtagtgc aggtcggccg cgacctcacc gagcacgtcc tgccccatga
     3241 gcttctgcac cggcaggttc tgcttgcgca tcgcccgggc gatcgcgtct ttgccggcct
     3301 cgatcgcctc gtcgcggcgc tccttcgtga accattgccg gatcttggtg cgcgcccgcg
     3361 ggctcttcac aaagttcagc cagtcgcggc tgggcgccgc gtctgggttc ttcgaggtga
     3421 agacctcgac gacgtctccc gtgttcaggg aactctccaa cggcacgaga cggccgttga
     3481 cacgcgcgcc catcgtgcga tgtcccacct cggtgtgcac ggcataggcg aagtcgacgg
     3541 gggtcgagcc cgccggcagg cctatcacgc ggcccttcgg cgtgaagacg tacacctctt
     3601 tcgcaccgat ctcgaagcgc agcgagtcga ggaactcccc tgggtctgcc gtctcggcct
     3661 gccagtcgga gatgtgggcg agccaggcga ggtcggcgcc gctggcgttg gcggtggtgg
     3721 cgacgccgcg cccgctggcc tgctccttgt acttccaatg cgccgcgacg ccgtattcgg
     3781 cccggcgatg catctcttcg gtgcggatct gaatctcgac cgggcgcccc tccggcccga
     3841 tcaccgtcgt gtgcaacgac tggtacaggt tgaacttggg ggtggcgatg tagtccttga
     3901 accggccggg catcggggtc cacagcgaat gcacttgccc cagcacggcg tagcagtcgc
     3961 gcaccgagcc gacgatcaca cgaatgccga cgaggtcgta gatgtcgtcg aactcgcggc
     4021 cgcgcaccac catcttctga tagatcgagt agtactcctt gggccgcccg ctgacctcgc
     4081 cgcggatgcg ggcctctttg agctcacgtt tgacgatctg gatgacctcg ccgacgacgc
     4141 gttttcgctg cggggagcgc cgcttcacca gggaatcgat ctcttcgtag atcttcggct
     4201 gcagcacggc gaacgagagg tcttcgagct cgagtttgat cgtctggatg ccgagcctgt
     4261 gggcgagcgg cgcgtagatc tccagggtct ctttcgcctt gcgctgcgcc gactcgacgg
     4321 gcacgaaacc ccaggttcgg gcgttatgca gccggtcggc gagcttcacg atgagcaccc
     4381 tgatgtcttt cgacatcgcg acgatcatct tgcgcaccgt ctcggcttgg gcgctctcgc
     4441 cgtaggtcac cttgtcgagc ttcgtcaccc cgtcgacaag catcgccacc tcgtcgccga
     4501 agtcgcgccg tagcgcgtcg agcgagtagt cggtgtcttc gacggtgtcg tgcaggagtg
     4561 ccgccgtgac ggtcttggca cccgtgccga gttccgccaa gatctgcgcc accgctacgg
     4621 ggtggatgat gtaaggctcc ccgctgtagc gcttctgccc ggcatgggca tgctcggccg
     4681 tcgtgtaggc ccgctcgatg agcgccgtgt cggccttggg gtggtgcgta cgcaccgtgc
     4741 ggatcagtcg ttcaacggcc tgggtgtgct gcgacctcga gaacaggcgc ggcacgagcc
     4801 ggcgcagcgt cgtggactgt tgcgttcgcg tgtccaccat cgccgtgcct ccccgatcat
     4861 tatcgtcgtt cgctgaagca tccggccagg aatggcgaac cctcggccgc ggcagccgcc
     4921 gccaggcggc tactcggcga cgagctcggg ctgctcactc gaggccgctt tggcgcgcag
     4981 ggcgagcacg cgcttgtcgt gcttcgccac cttgtcttcg ccctctcgga actcggcata
     5041 gagcggcgag gcgatgaaga tcgtggagta cgtgccgagg atgatgccga taaagagcga
     5101 gagcgagatg tcgcgcagcg tgtctgcccc gaaaacgagc gtgccgatga agagaatggc
     5161 ggcgaccggc aagacggcga tgacgcccgt gttgatggac cgtaccagcg tctggttgac
     5221 ggccaggttc accgactccg cgaaggtgcg tttgagcttc gtcgcgtcat cgaccgtgtt
     5281 ctcgttgatc tggtcgaaga cgacgaccgt gtcgtacagg gagtaggcga gaatcgtgag
     5341 aaagccgatc atcgtcgccg gggtgatctc gaacccgacg gcggcgtaca cgccggccgt
     5401 gatgatcatg tcgtgcagca gcgacaggat cgcggcggcc gacatcttcc aggtgcggaa
     5461 gtagagcgcc atcgcgatga acgcgagcag caggaacacg acgacgccct gaatggcctt
     5521 cttgctgacg tcagcgcccc agctgggccc gatgaacgtg gacgtgacgt cggcgacggg
     5581 aaccttatag gccgtggcca acgcctggcc cacctcgagc gacttctgcg aggtgagctg
     5641 ctccgtctgc acctgcacgg cgttgccgtt gattgtcgtc accgtggccg tgatgctcgg
     5701 atcgacgctc gcgaccgcgc ttcgcgcgag cccctgatcg gtcgtcttca cctgcgtgat
     5761 ggagaactgc gagccgccga cgaagtcgat gccgaaattg aatccgcggt gaaagatcgg
     5821 gatgagcacg gaggcgatga tcaagacggc cgcgatggag taccagatct tgcgccggcg
     5881 gtggccgacg aagtcgatcg agagggcccc cgtgtagagg tcgttgccga aacgctgaaa
     5941 gcgggtaggc atcaggattc ctttccgccg gttccctgcc cgaccttggc cgcctcgcgt
     6001 tcggccgcct tgcgttccgc gatggtctga cgcttggcgg cctcgcgccc gctggaggcg
     6061 agcttggact cgcgcctctc ggtgacgtgg gtgcggaagt ggcctgcgcc gcggtagacg
     6121 gcgacgccgt gtgcctcagg gtcgagaccc gacatcttgt gcccggtggc gaagtagttg
     6181 gtgcgcgcca ggagccgcaa cgtcggatga gtgaacagca gcacgatgat gacgtcgatc
     6241 acggtcgtga cgccgagcgt gaaggcgaag ccttgcacgt cgccgacggc caagacgtaa
     6301 agcacgatgg cggccaagag gttggtggcc ttcgacgcgt agatcgtgtg cctggcgcgc
     6361 ttccagccgg cctcgacggc agaagcgaga cccctcccct cgcgcagttc gtcgcgtatt
     6421 cgctcgaagt agacgatgaa cgaatccgcc gtgaaaccga tggcgacgat gagcccggca
     6481 acgcccgcca gcgagagccg atagtcgatc cgccacgaga gcagcacgat gacgaggtag
     6541 gtgatcaccg cggagccgag gagcgacgtg atcgtcacga ggcccagcag gcggtactgg
     6601 aagagggtgt acaagacgac gagcccgagg ccgatgaggc ccgccaagag gccgctcttc
     6661 aactgggagg agcccagggt cgaggagatc gtgttgttgc tctgcacccg gaagctgacg
     6721 ggcagcgccc cgaacttcaa ctcgttcgcg aggttcttgg cgctcgtctc cgtgaagcct
     6781 ccggtgatct gcgcctggcc gttcgtgatg atgccgttca tgctgggcgc ctcgaggacc
     6841 ttaccgtcga tgacgaaggc gaaccgtgcc cgcgggtcgt tctggttggc ctgctcgagc
     6901 gcatacagtc gcttgctgac cgtggcgaaa ttggtcttgc cctggccgct gaaggtgacg
     6961 ttgaccgccc actcccccgt cgtcacgccg ctctgcgacg tgacgaggcc ggcagaggcg
     7021 tcggagatgc tggaaccgtc gacctcgacg gggccgagga tgtacttgtt ctgcccggcg
     7081 tcatcacagg tgacgagcgg cttgttcggg tcttgcacgc tcgtgagctt gttctccgcc
     7141 gtcttgcaat cgaggtcggt gaacttctgc tgaagggccg gcgtcaccca ggccgggtcg
     7201 ctgccgttct tcggcttcgt cttcggcgtg ttgctgaggg ctttggcgcc agcggtcgcg
     7261 ctcgggctcg gcacgggcgc ggtcgccgca ccggcgacga gaaccggccg caactccatc
     7321 ttcgacgagg cctcgatgcg ctgcctcgtc gagttgtcga ggtggcggcc cgggatcgtg
     7381 acgtcgatgt tgttgccgct ttgcacgttg atctgtgcct cggagacccc ggaggcgtcg
     7441 atgcgctgcc ggatgatcga caccgcctgg ttgagctcgt cgctcgtgat cttcgcgccg
     7501 tgctccaatt gcggtgccaa gatgatctcg gtgccgcctt cgaggtcgag gccgagcttc
     7561 ggcgcccagc cggcatgcga aaactgcacg cctcccgcca ggacgacccc gagggcgacg
     7621 acgatggcgc ctagccagat cagcgcgcgc catgcgctct tcaccggagt cgacttggcc
     7681 acgggcagct cagctttctt tccgcggcgc atgaaggctc gccgcgttca ggttagagac
     7741 gaagaagtgg gtcttacttg ttgtccgcgg aagggcgttc gccgtcgacg cgctccccga
     7801 actcgggctc cgcgtcggcg gggcgggcgt cgtcgaacga gccttcccga gcctcaccgg
     7861 cggcatcatc ggcgggctct tcgtcgggcg tcacgacccg cgagacgacc tggcgatgca
     7921 cccgcaagat ggtgcccggg gtggtctcta agagcacctc gtttttctcc tcgtcgatgt
     7981 cgatgacgcg gccgaagacg ccgaagttcg tcatcacgtc ggcacccgtg acgaccttcg
     8041 agcgcaactc cgcctgttcg cgctggcgct tcttgccgtt gcggaacatg aagaaaacca
     8101 tgacggccaa gacggccaac atgacgtaca taatcgggtc catgactaga aaacctccgc
     8161 cgcgtcaggc accaacacta gtgtgtcggc acgaccagtg tgaattatag gtcaccgatg
     8221 ccgggcaggc cctggctcgc ggcacgctcg gcgggcggca cacgcccgaa gtgctgccac
     8281 gccgcccgag tcgcgacacg gccgcgcgga gtccgggcga tgaggccggt gcgtaccaag
     8341 aagggctcga cgaccgactc gatcgtctcc ggctcttcac ccaccgacac ggccaacgtc
     8401 gagagcccca cgggacctcc gtcgaaacgg tccaggattc ccgtcatcac ctcgcggtcg
     8461 agccggtcaa gccccatctc gtcgacgtca taaagttcga gggctcgacg aacggtgtcg
     8521 agacccaccg gcgtgccgtg cacgagcgcg tagtcgcgca cccggcgcaa caggcggttc
     8581 gcgatacgcg gcgtgccgcg gcagcgcccg gcgatctccg ccacggcgcg cccgtcgatc
     8641 gcgacgccga gcttcgcagc ggtgcgggcg acgacctcgg caagctcctt aggcgcatag
     8701 aactccaggt gcgcggtgaa cccgaacctg tcccgcagcg gattcggcaa gaggccggaa
     8761 cgcgtcgtcg cgccgaccag ggtgaaaggc gcgaggtcaa gcgggatcga cgtcgcaccg
     8821 gcccccttgc cgaccatgat gtcgatgcga aagtcttcca tcgccagata gagcatctcc
     8881 tcagccgagc gcgccatgcg gtggatctcg tcgatgaaca gcacctcgcc gggcgtcagc
     8941 gacgacagga cggcggccaa gtcgccggca tgctggatgg ccggcccgct ggagatgcgc
     9001 aacgcatggc cgccctcagc tgcgacgatc atcgcgagcg tcgtcttgcc gaggccgggc
     9061 gggccggcca gcaggatatg gtcggccgta cgcccttgta acgcggccgc ctcgaggagg
     9121 agacgcagct ggcccttgac cttctcctga ccgacgaact ccgcgaggga atgcgggcgc
     9181 aacgccccct cgaaggcgag ttcggcgtcg ccttcgggcg acgggtcgac gacggtgccg
     9241 ttcaccgggc cacccgctcg gccggtcggg cgcccggaac catgcgccgc agtgcgagcc
     9301 gcagaagggt cgggacgtcg gcgtcggcgc cctcggagcc ccgggcctcc tccaccgtct
     9361 cgcgcgcgac ccgctccggc cagccgaggc cgacgagcgc ctcgacgacc gcagcgtcga
     9421 cgccgccggc ggcgggtgcc gcttctcccg ccgagggggt gcggaagtgt tccatcttgc
     9481 cggtcagctg cacgacgatg agcttcgcgg tcttcgtgcc gatgcccgag accgcacgga
     9541 acgcggcatc gtcctcccgg gcgaccgcct cgccgatctg cgccgggctc agctcggtga
     9601 gaacccccat cgcgcttttc ggcccgacgc cgttcacgcc cgtgagcaag tcgaacacgg
     9661 cccgttcatc gacgcccacg aacccgtaca ggctcacggc gtcggcacgc atcacgaggc
     9721 gcgtgtagac gagcgcctcg gcgcccactc gcaacctgct tgcgtgccgc ggcgtgacgg
     9781 cgacggtgta tcccacccca cccacctcga gctcgaccgc agtcggtgtg acggcgacgg
     9841 ccgtgccgcg cagcgatgag atcaccgacc cagcctacgg cccgccccgg cctttctctc
     9901 ggcctcgcgc cagcgccgca aggcgggcgt ggcgagttcc gaccccgcgg cgccctgctc
     9961 tcctgtaccc gccggccctg ttccgccgcc ccaggcgtcg cacaacgcga tggccaaggc
    10021 gtccgcggca tcgggcggcg agggcggggc ggcaagcccc aggatccgtg ccaccatcgc
    10081 ctgcacctgc gccttgccgg ctctgccata gccggtcacg gcagacttca cctgggtggg
    10141 ggtgtggatg ccgacgggca ggccgcgacg ggtcgccgcc gcgacgagga tacctgagat
    10201 ctgcgcaacc ccgatgacgg tgcgcacgtt gtgctgcgcg aagacctcct cgatggcgaa
    10261 agcctgcggc tgatattcat cgatcagcct ctccacaccg tcggcgatcg cgacgagccg
    10321 ggcgccaagc ctggcctgcg cagcactgcg taccacgccg acataaacga gggctgcgga
    10381 acgggtcggg ccgacgtcga tgacgccgac cccgcaacgg gtcagccccg ggtcgatgcc
    10441 cacgacccgc cgggtcacgg tgccggctat gcgtcttcca tgtcgagctc agcctgcacg
    10501 gcggcgctca gctcgatgtt ggtgaagacg ttctgcacgt cgtcggagtc ttcgagagcg
    10561 tcgatcagcc gcagcacctt gcgggccgtc tccgtgtcgg cgtcgatgct gacattggcg
    10621 acgaactcga cgtcggcgga gtcgtagtcg atgcccgcct cttgcagggc ggtgcgcgcg
    10681 gcaaccatgt cggtcggttc ggtgacgacc tcaaacccgg ccgcgtggcc cgtcacgtct
    10741 tcggcaccgg cctcgagcac ggcggccagt accgcgtctt cgtcgacccc gtcggcctcg
    10801 ggcacgacga tgacaccctt gcgggtgaag ttgtaggcga cgctgcccgg atcggccatc
    10861 gtgccgccgt tgcgggtcat cgccgtgcgc acgtcggccg ccgcacggtt cttgttgtcg
    10921 gtcaggcatt cgatgaggaa ggcgacgccg cccgccccgt aaccctcata catgatcgtc
    10981 gtgtattcga ccgactcacc ggtcaggccg gcaccgcgct tgatggcgcg atcgatgttc
    11041 tcgttcggca cggaggtctt cttggccttc tgcaccgcgt cgacgagcgt cgggtttccc
    11101 gagaggtcgg cgccgccgat cttcgccgcc acctcgatgt tcttgatgag cttggcaaac
    11161 gacttcgcac gacgttgatc gatgacggcc ttcttgtgtt tcgtcgtcgc ccacttggag
    11221 tgccctgaca tacctctccc gttcccgctg cgcgtgcctg catagacatt ctaaggtgcc
    11281 ggccccgcgg cgcccaccgg cggcatcgca cgaccggcgt cacggccggc ctctcgtggt
    11341 gtcagacccg cgcgagcacg tggtcgcggt attcggccac cttcgccaga aaatagcggt
    11401 ggaaccggtc ttcaccggtc atctccgggt ggaacgacgt gccgatcagg ttgccctgct
    11461 cgacagcgac gacgcgccca tcggcaaggc acgccaaagg ccgggcgcca gggccgagcg
    11521 actcgacgat cggggcgcgg atgaagacgg cgtgcaccgg ccgatcgccc tccagccgcg
    11581 gcacgtcgag gtcgacctcg aacgagtcga cctgcgagcc gaacgcgttg cgtcgcacgc
    11641 agatatccag gccgcccagg ctctcctggc cggcgatgcc gtcgaggatc gtctcggaga
    11701 gcatgatcaa gccggcgcag gtgccgtaga tgggcatccc gtcgcgaatc gcctcgcgca
    11761 acggctccgc cagcccgaac tgccgggaaa gcttgtccat gacgctcgat tccccgcccg
    11821 gtatgacgag cccatagacg gaggcgagct cgtggggccg gcgcaccggg atcgcgtcgg
    11881 cgccgagagc cttaagcacc gcgaggtgct cccgaaagtc gccttggagc gccaagaccc
    11941 cgacgcgagg ctgcgccgcc tctcccggcg cagcgggcgc gggtgtcacc gagacgtcac
    12001 cagcctcggt ctgccaggtg gtggggcgcc ggaatgtcgg agacgttgat gcccaccatc
    12061 gcctcgccga gaccgcgcga gacctcggcg atcacggagg cgtcctcgta gaaagtcgtc
    12121 gccttcacga tcgcggcagc ccgtttcgcc gggtcgcccg acttgaagat gccgctaccg
    12181 acgaacacgc cgtcggcgcc caactgcatc atcatcgccg cgtcggcggg cgtcgcgatg
    12241 ccacccgccg tgaaaagcac gaccgggagc ttgccgagcg acgcgacctc ttgcacgagg
    12301 ccgtagggtg cctgcaactc tttcgccgcg acgtacaact cgtcgtcact catcgccgtc
    12361 aacgccttga tctgggcact gatcttacgc atatgcttgg tcgcctcgga gacgtcaccg
    12421 gtgcccgcct cacccttgga acggatcatc gccgccccct cggtgatgcg ccgcaacgcc
    12481 tcccccaggt tcgtcgcgcc acacacgaac ggcaccgtga aacgccattt atcgatgtgg
    12541 ttcacgtagt cggcggggct caacacctcg gattcgtcga cgtagtcgac gttcaaggcc
    12601 tgcaagacct gcgcctcgac gaaatggccg atgcgagcct tcgccatgac gggaatcgac
    12661 acctcatcga tgatgccctc gatcaggtcg ggatcactca tgcgtgcgac gccgccttga
    12721 gcgcgaatat cggccggcac gcgctccaac gccatgaccg ccaccgcgcc cgcatcctcc
    12781 gcgatccgcg cctgctcccg agtgacgaca tccatgatga cgccgccctt caacatctcg
    12841 gcaaggcctc gcttgacgcg gttcgagccg aacttcgtct cactgcccgt taccgcgttc
    12901 tcctgcgcca ccatcactcc tcgtcctcgg gtcaggccaa gcgaaccgcc aggccgtgcc
    12961 gtatgccaca ccgcccggcg ggttctcagt ttactctgcg gctgttttcg gccacgcggc
    13021 cgcgatcgcg tcgcgcatct ctcccaagag ttgcggcagc gccttcgtct tcgcgatgat
    13081 cggcagaaaa ttcgcgtcgt gcgcccaccg gggcacgatg tgctggtgca gatgctcgga
    13141 gatgccggca cccgccaccc ggccttggtt cataccgatg ttgaacccgt cgttcgcgct
    13201 cacctggcgg atgacccgca tcgccgtctg cgtgatctcg ccgatctcgg ccacctcctc
    13261 ggcgctcgcc tcgtcgtaca acgcgacgtg ccgatacggg cagacgagca gatgcccgct
    13321 gttgtaaggg tagaggttga ggagcgcata ggcgctcttg ccccgataca cgatgagtgc
    13381 agcctcgtcg ctgcgggacg gggctgcaca aaaggggcaa ccgtgggcgg tgtggtcggc
    13441 gccctcagcg atgtaggcca tgcggtgcgg ggtccacagg cgctggaact cgtcgggcac
    13501 accggtcagg cggccgggat cgacgacgtc gatcaccggc tcgccggaac ccgagaggtc
    13561 ggccgtcgtg ccgagccccg ttcgaccttc tgctacgccg gccacgcggt cacgacctgc
    13621 tcgtgcgcgt cgatcgtctg cgtgatgcgc cggatcgcct cgtcgacggg tacgccgttg
    13681 cgctgtgagc cgtcgcggaa ccggaagctg acggtgcccg ccgcttggtc ctcgtcgccg
    13741 gcgatcaaaa ggtagggcac gcgctggcgg gtgtgagtgc gaatcttctt ctgcatgcga
    13801 tcgtcggagt cgtcgagcac cgcacgcaca tcgcgctcgc gaagcctgcc cagaatgccg
    13861 gccagatagt cacgatggtg gtccgcgatg gggatcccca ccgcctgggt gggcgcgagc
    13921 cacacgggga aggcgccggc gtaatgctcg agcaggatcg caaagaagcg ctcgatcgac
    13981 cccaagagcg cccggtggat catgacgggc cttttgcggc tgccgtcagg cgccgtgtat
    14041 tgcaggtcga agagctccgg ctggttgaag tcgagttgca ccgtggagag ctgccacgtg
    14101 cgaccgattg catccctcgc ctgcacggag atcttcgggc catagaaggc ggccccaccc
    14161 ggatcgggca caagctccag gccggagccg agcgcgacct cgcgcagcgt ctcggtcgcg
    14221 gcctcccaca ccgcgtcgct gccgacatac ttctcgggat ccttcgtcga cagttccagg
    14281 tagaagtcgt cgagcccgta accccgcagg gtggcgagca tgaaccgcaa ctgccccgcg
    14341 acctcgtctt tcacctggtc ttcggtgacg tagacgtggg cgtcgtcttg cgtgaggccg
    14401 cgcacccggg tgaggccggc gagcgtgccg ctcttctcgt atcggtagac cgtgccgaac
    14461 tccgccaggc gcagcggcaa ctcgcggtag ctgcgcccgc gcgcccggaa tatgaggttg
    14521 tgcatggggc agttcatggg cttcaggtag tagtcctgtc cctgccgggt gatgttgccc
    14581 gcctcgtcgc gctcctcgtc gaggtgcatc ggggggaaca tcccctcctt gtaccaattg
    14641 aggtgctggc tgatctcgaa gagatggcct ttggtgatat gcggggtcga gacgagctcg
    14701 tagccgttgc ggagcagctg ggtgcgcagg tagccctcga tctcggcacg cacgatgccg
    14761 cccttggggt ggaagacggc aaggcccgaa ccgatctcgt cggggaagct gaacaggtcg
    14821 agttcagcgc ccagtttgcg gtggtcgcgc ttcgcggctt cttccagccg cgcctgatac
    14881 gccttgagat cgtctttcga ggcccacgcc gtgccataga tgcgctgcaa ctgcgggttc
    14941 ttctcgctgc cgcgccagta ggcggcggcg acccgcatga gatcgaaggc ccggccgatc
    15001 atgcgggtgt taggcaggtg ggggccgcgg cagaggtctt tccagacggt ctcgccggtc
    15061 gccgggtcga cgttgtcgta catcgtcagc tcgccggcgc cgacctcgac actctcgttg
    15121 tcggtcgcat tcgcgacatg tgctttcaac ccgatgagct cgatcttgta cggctggtcg
    15181 gcgagctccg cccgggcttc gtcttcggtg acgacccggc gcacgaagcg ttggccctgc
    15241 cgcacgatgc gctccatcgc cttgcggagt gccttcaagt cgtcgggcgt gaacggggcc
    15301 gccacgtcga agtcatagta gaagccgtcg gcgatcggcg ggccgatgcc gagcttcacc
    15361 tccggattca cagattggac cgcttgcgcc agaacgtggg cggccgagtg ccgaaggatc
    15421 gccagaccgt cgggagaggt gatcgtgacg ggttccacgg tgtcttgcgg tgtcacggtg
    15481 gcggcaaggt ctttgagaat accgttgacc cgcatcgcca cgaccgtgcg gtccgtgaaa
    15541 agctcgaacc cgtctgccac ccgtctgcct ctctgttgcc cgaccttctc actgtagtgg
    15601 gtggccgaag gcgagaggcc cgcgagggct ggcgctactg tcggtgcagc gcgaggaccg
    15661 ggatcgtgcg ggtcgtcttc tcttgatacc cggtgtatcg gtcactgaac gagacgaact
    15721 gcgcccatgc cttctctcgc gccgggccgg agaggtcttc agcccgcacc ggcacagtgc
    15781 cgtcgtcggg cgtctcgatc tgtacgtcgt cggggtgggc gacgaggttg tggtaccagg
    15841 ccggattgtg tggcgccccg ttggcggagg ccgagagcag ccaggtcgac gggtcggggt
    15901 gccgagcgat gagcggcgtg acccgttccg tgccggtctt cgcaccccgg tggtgtagca
    15961 gcacaaggtt gcgaccgaag atacgggtcg tgccgccgcc cgaacggaac tcttcgatga
    16021 tggaacggtt ccactcgcgc gggtcttctg ccataactgc aggctaccgc ggccagacgc
    16081 ccgacacgtt cggtgggcga tactgggttc gaaccagtga cctcttccgt gtcagggaag
    16141 cgcgctacca ctgcgccaat cgcccagtct tgatatctct cggctggaga ggtggatacc
    16201 ggattcgaac cggtgtatac ggctttgcag gccgctgcct cgcctctcgg ccaatccacc
    16261 gtttcgagtt cgccaccgaa caagagggca cagaatcgtg gacccctcga gcggatgacg
    16321 agattcgaac tcgcgaccct caccttggca aggtgatgcg ctaccactgc gccacatccg
    16381 catgcatgtt tccgcgtgct tagtgactct aaccgaaaac gcgagctttt tccaaacgct
    16441 ggagcgtcgc cctgcgaccg gtcgtcacac gtcggcgagc acgccgtgtg cgtcaagggt
    16501 cgcccagata cggcagtcat cgatccgggc gcgcagcgcc ccccagcccc acgccgggcg
    16561 gcggtctagg cggcctcgac aataggcgcc gcacccgcag acggggagca gaagaggatg
    16621 cgcggctacc cgcagggagg gggcgccggc cgcacggtcg acttccgcta tgatcggtgg
    16681 gcgggccgca ggcgtgcggc gcgggcgatt ggcgcagtgg tagcgcgctt ccttcacacg
    16741 gaagaggtca ctggttcgaa cccagtatcg cccaccagac aggcggcgct tctcggaatg
    16801 acgggaaaac cagtgacctc gcgagaatcg gcgggagcga tgctcccgcc ccaagattct
    16861 cgcgctggtt cgaacccagt atcgcccacc acacaggccg cgcctctcgg aatgacgcgg
    16921 aaaccggtgg ctgcctggca ctcgaggtat cgactcggcg gcacggcggt cggcgcggcg
    16981 acgagagtcg cgacgaaacc ggtcggccag cgagacggcc gggcggatcc acccgcctgt
    17041 tcgctatcag agggccacgc cggttgcccg cgccgcacga ccgacggaag ggccatcgtg
    17101 accgccgtcg cccgcaacac gcaccccggg gcccagcagg aagacacgac tccggcccgc
    17161 cgatgcggtg cccaggtcac ggggaatgct cagggccgag cgcgcgagac cgcggaatcg
    17221 ccgcaacgct cgcccccaat aggcgactgg ccgccggcca catatccgta gcgccgcggc
    17281 atggtcgacg cagagcacgc tcgcggcacc gaccgcgaca acccaccatg cggcgcctgc
    17341 gatcgccttc gtcacacgag aggcgtctgc gaatccgacc cgcagcctgt ccacctggaa
    17401 tcgaagatgc tcacgttcgt cggtgcgaat gcgttcaccg ataccgcgca gcaccgggtc
    17461 aggtgcgctg tctgcaagag cttcgaaata ctcgatcgcc accgcctcgg cgatcaagaa
    17521 cagcgcgagc tccgtacgca gcccgagcag ccgacgcagg cgcgtgaagg cgacgtcgga
    17581 ccagtgccct cgcagcgacg gcgccttcat gcggcgcagc ccgacaccga acagggcgga
    17641 atgcttctgc tcctcgacca cgagcagctc caacgcccgc gtgtatgcgg ggtcgcccgc
    17701 cgcgcgcgcc ttcgccagga gatgcacgcc gtcaccgccc tccccgagtt cgaagcgctg
    17761 aaaggagcgc accagtgccc gccgctgcct cgtcgtcaac acaacgggcg cggcgggatc
    17821 ccacgctcgt tcgagccgcc gctgccgctc cggattcgcc tgaaaatagt ccacccagac
    17881 ctggaaaccg ggaggatagg cttcgtcgca cggttcgacg tcacacacca ggaggtcgtg
    17941 cgcggcacca gtcgtgcacg gcggccgtgt cacgctcgaa gtctcactca tcacaggctc
    18001 cttcgataaa tagctattga tatacgatcg gtatttaccc ataatcgata gtgtgagtca
    18061 agtcatcatc gttttcattc gcgtcttcat cgggctgatc gcgttgggca cgctcgccgc
    18121 acaggtgctc gtcatccccg agttcgccgc cgagctcggc accgtgtcgg acatgccgtc
    18181 ggcgagcgtt ccctacgccg tcgcgggaat catcgtcggc gcgtgtttcg aggtggccct
    18241 cactgccacc tggatgctgg tgtccatggt gagccgcgac gcgatcttca ccgagaacgc
    18301 ctttcgatgg gtagacgcga tcatcggcgc ctcactcacg gcgctggcac tcgtcgtggc
    18361 cttcggtgca cacgcggtgt tcgtcctcca gccccggctc gactcaccga gcatcgagct
    18421 cgtcgtcggg gccaccgtga tatgcggcgc cacattcgtg ctgctcgtca tcgtgttgcg
    18481 cggtctgttg cgcaacgcga cgaacctgcg cagcgaactc gccgaggtca tctgatggcg
    18541 atcatcgtcg acctcgacgt catgctcgcc cgccggaaga tgtccgtcgg cgacttcgcg
    18601 agtgcgatcg gcatcacccc ggccaacgtc gccgtcctca agaacggccg cgccaaggcg
    18661 gtgcgtttca ccacactcga cgccatctgc cgcgttctcc aatgccagcc cggcgacctg
    18721 ctccgctggc aacccgacga cgagtgacag cgagcacgtg aggtctgcgt ctctcagccc
    18781 acacgacaac gtacgctgtc gattcgcgcc gtctgggcgt gcgcagactt gcggcctgtg
    18841 cgccgcctcg cctcacccgg tcgtcagtcg gcggccttcg cgaggaagcg ctgccaggag
    18901 gtaggcggcg ccgaccgcga cagtcgcgcc gatcacgatc cacccggcat gcatggttgg
    18961 aaggagggag ctgagcgggc ccgtatcgcc acgatcgcgg cgaccaccat ggccgccaga
    19021 gagaccagcg acaagaccac gcggccgacc cagcacccga ggtccgttgc ctcccgggcg
    19081 gctggcggcg actgggaggg cgcaaaacga ctcacgtagg gcggcgagca ggccacaagc
    19141 cggcgtcgtc acaagggctg tagcgagaac gtgatcgtga tcggatctga cctggaggtc
    19201 agggtgagga tgccggcaag attgagccgg ctcggggaag ggacgatcac ttcaagcgtg
    19261 cccgggaggt tggcgctgta atcgatggat tcccgcaccg cgaccgtaac gccgcgatca
    19321 tcggtcacga cgtagtccgg gcgcgaggct gtgctgcgca agatggcgtg gcgaccaccg
    19381 agcatctctt ctcgggtgcc gccacgcagt cggaggatca cgaggtcgtg cgtttcgtcg
    19441 gcaaaaacga tgtccacaga tgggtccgaa gcggacacca ccgtccccct gtagagccgc
    19501 atcggctcac gctactccgt gaccccatgg gctgtgtcgt agcggtctac acccttcgaa
    19561 tccagccccg catacctctg ccgacttgcc tggtatcgag cagaattcga cttgtgaggt
    19621 cgtgagagac gttcccctcg ttgtgacagg accgccgcag gactgccatg tcccaccacc
    19681 ggtctcgtat tcgcgggtca gtgtcttgag acccgaaacg agagaactgg cgtcggccct
    19741 tgccttatac acgctcgggg cgagagtcag acgcgccgca agatacccca caccgggaat
    19801 agtggtgcac cgtcgtctcg ggccgtgggc acagcgtgtg acgtatagct acatcatgtt
    19861 gcgcactcag atctcactcg ccgtcgcggg cgacgggggc ctcaagtgcc gtccgtcacg
    19921 catgcgcagc ttcatcgaga cgggtagtgc ggcgaaggca gcaccgatca gcgccgccat
    19981 ggcggttccg ccccggctca tgcggccgga gcgcgccaac catcgggcgg aggcgacggc
    20041 gtgcggggcc atcaacacga cggccgtggc aaggcccggg ttgtatcgac gctgccgaac
    20101 cgccatgccg gcgtgcatgg cgacgttgcc ggcctccatc gcgacaacgc ccgcggcgac
    20161 accggggctg cgcttccaca gcaacatccc cgcgaccgca ctgccccacc cggccgtcac
    20221 gttcacgtga aagccgacgc gcctcgtcag cggccaatcg ggccggcgcg agccgagcag
    20281 ccgcttgttg gcgaacggca agaagccgcc cggcctcacc cattcctcgg tctgatgcgc
    20341 caagaggatg ggcaaggcga gccctgcgac ccatccgccg tcgcgggcca gccggcgcct
    20401 gttggcgagc gccgccgccc atgccgggca gagcgcagcc gctgcgacgc gcggccatcg
    20461 tccgtatgtc tcaatcacga tgtcgggttc ctctccgtct actcggggtg gtgcgcggga
    20521 atttacgacg cgatcggcga cgatcaggag tcgaggtcgc cgtacggcgg ggtggtacgc
    20581 gggagtctac ggcgagcatc gcccgcgatc aggaattgag gtcgccgtac agctcagcca
    20641 agagcggttc gatgtggtcg agttccgcga ggtgtgcgcc ggtcagctcc gcgagcttct
    20701 cggcccccac cgcggtcaag gtgagccgga cgatccggtg atcatcccgg tcgcggtggc
    20761 ggcacacaag accgagcgcc tcggtgcggt cggccagccc gacagcggcc gcatggccga
    20821 tcatgagata gccggcgatc tgcccgatcg tgggaggctt gtccccctta tggccgcgga
    20881 cggccaacat cagctggtgc tgcgccgacg tcagaccccg tgcagccgcc tgttgcgcgc
    20941 tccagtactc gaactcacgc agccgcgtgc gcagcctgag gagcttggca tagtcgcggt
    21001 cgctgacatc cgttcgcccg gtcacagaca cgagacctcc gtctccgacc ggacggccca
    21061 ccagacgata aggcgactgg tccaacgcac cggcaccaac atgtatcgta taacgattac
    21121 ttcacgccgt tgtataatcg tggccgaatg taacggagtg tggttccaca ccccgtgatg
    21181 acgaaagtga gacgtaccgg tggttcacgg ttcgcggggt cgacgggtcg acagaaagaa
    21241 cgaggtcagt ggagagaacg ggtccggg
//
LOCUS       DASTNL010000073         5481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_54294, whole genome shotgun sequence.
ACCESSION   DASTNL010000073 DASTNL010000000
VERSION     DASTNL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54294"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="upp"
                     /locus_tag="VFQ96_02525"
     CDS             complement(<1..573)
                     /gene="upp"
                     /locus_tag="VFQ96_02525"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU0256695.1"
                     /translation="MRVHVADHPLITHKLTMLRNRDTASVTFRSLVEELVTLLAYEAT
                     RTVRVAPVEVQTPVALAHGVTIADPKPLVVPILRAGLGMLGGMVKLVPSAEVGFLGMM
                     RDETTLRPTIYAERLPENLAGRQCFVLDPMLATGGSLGTAIRFLLQRGATDVTAICIL
                     AAPEGIAAVEKLTAGLNVSLVVGGVDDHLNE"
     gene            706..1155
                     /locus_tag="VFQ96_02530"
     CDS             706..1155
                     /locus_tag="VFQ96_02530"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU0256696.1"
                     /translation="MAKALVEARRSLSSADVPVGAVVVDAKGRTIGRGHNRRQELFDP
                     TAHAEILALREAARASRSWRLAGATLVVTLEPCAMCAGAALAARVARVVFGAWDAKAG
                     AAGSVYDLPRDARLPVQMEVYGGVDEASCARLLTEFFEARRGPDGQG"
     gene            1187..2158
                     /gene="proC"
                     /locus_tag="VFQ96_02535"
     CDS             1187..2158
                     /gene="proC"
                     /locus_tag="VFQ96_02535"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU0256697.1"
                     /translation="MISRANPGSPVPVGPAASGAHADAGTDAPAGGRPAAGADRPLVV
                     AILGTGSMGTAVLRGLTGPEAGPAEIRVTTRSAAHAAALGGQDGVTSYAVESDPDANR
                     LAARGADLVVLAVKPHLIPGLAGEIGDVIDAHTIVVSVAAGVAIATIAGRLPAGVAVL
                     RAMPNTPAVIGRGVTGLSVTGGRGSAAALRVVHDLFARVGEVVEVPEDRLDALSAVSG
                     SGPAYVFLLIEKLTAAAVRLGFAEREAARLVAGTFRGAAELLAAGEAAPAELRRQVTS
                     PHGTTERAIAVLEEAGLDDLFGRALEAALARARELAAEGASRSAPEA"
     gene            complement(2321..2992)
                     /locus_tag="VFQ96_02540"
     CDS             complement(2321..2992)
                     /locus_tag="VFQ96_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU0256698.1"
                     /translation="MVDRIRHDAPVLVIGLGRFGAATAGQLDRLDREVLALDIDSALV
                     QKWSDRVTHTVQADARNIDALKQIGAGEFSIAVVAVGSSIEASVLITANLVDLKIPQI
                     WTKAISQSHGKILERIGASHVIYPEAEAGERVAHLVSGRMLDFIEFDDDFALVKMYPP
                     RSIRGLTLAEANVRHKHHVTIVGVKSPGKPYTHAAPETTISDHDLIIVSGESADIEHF
                     ASLDH"
     gene            complement(2985..4421)
                     /locus_tag="VFQ96_02545"
     CDS             complement(2985..4421)
                     /locus_tag="VFQ96_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEU0256699.1"
                     /translation="MPRTGSPRQPRVGRPGGLRGWLSHVTGRSPARFAVVVFTLLILV
                     FTVLLSLPAASATHTVTPLSDALFSAVSVVCVTGLSTVDMATHWSLFGHVIIYVGVQI
                     GGLGVLTIATMLGTVISRRLGLRAKLLAASEGNPLRLHRGPVAEGQEVRLGEVGNLLA
                     TVAISAAVIEAAVAVLLFPRILAHGRSVTEAIFNSIYFSAMAFTNTGFTPDPEGLTPY
                     ATDYWFLTVLMAGVFLGAIGFPVLLAFARNLRARKKHWSVHVRLTLFAFAILFVAGGV
                     LYFVLEFDNPHTLAGLGAGDAIYQSFFLSAMTRSGGFATIDVGHLNNSSLLITDMLMF
                     IGGGSASTAGGIKVTTLAVLFLAAVAEAKGDDTMSAFRRTIPHDVLRLAVSVVLWGAT
                     TIAVSTILLLNITGQSLDDVLFESISAFATTGLSTGITQHLPPLGSYILSGTMFMGRV
                     GTVTLAAALAQSQRRQLFHHPEERPIVG"
     gene            4586..4819
                     /locus_tag="VFQ96_02550"
                     /pseudo
     CDS             4586..4819
                     /locus_tag="VFQ96_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189414.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5225..5323
                     /locus_tag="VFQ96_02555"
     CDS             5225..5323
                     /locus_tag="VFQ96_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEU0256700.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRKTRHQRRNKK"
BASE COUNT          814 a         1845 c         2006 g          816 t
ORIGIN      
        1 ttcgttcagg tggtcgtcga cgccgccgac caccagagac acgttcaagc cggcggtgag
       61 cttctccacc gccgcgatcc cctccggtgc cgcgagaatg cagatcgccg tcacgtccgt
      121 cgccccgcgt tgcaacagaa agcggatggc ggtgccgagc gagccacccg tcgcgagcat
      181 cgggtcgagc acgaagcatt gccggccggc caggttttcg ggcagtcgct cggcgtagat
      241 cgtcgggcgc agggtcgtct cgtcgcgcat catgccgaga aagccgactt cggccgaggg
      301 cacgagcttc accatgccgc ccagcatgcc gaggccggct cgcaggatgg gcacgacgag
      361 cggcttgggg tcggcgatcg tcacgccgtg cgccagggcg accggggtct gaacctcgac
      421 gggtgccacc cgcacggtgc gggtcgcctc gtacgccaga agcgtgacga gctcttccac
      481 gagggagcgg aacgtgacgg aggcggtgtc acggttgcgc aacatggtca gtttgtgggt
      541 gatgagaggg tgatccgcca cgtgcactcg cataggctca aatctaacgg cccggtcgac
      601 gccggccgtc gcgtgttcgg cggcccggcc cccgccgtgg tgctgggccc gtgagggagg
      661 cgttcattcg tgggcatcgc cgtgccgcag cgttatcgtg cctggatggc gaaggctctc
      721 gtcgaggccc gccggagtct ctcctcggcc gacgtgccgg tcggcgcggt cgtggtcgac
      781 gccaaagggc gcacgatcgg tcgcggtcac aaccggcggc aagagctctt cgaccccaca
      841 gcgcacgccg agatcctcgc gttgcgcgag gctgcccggg ccagccgttc ctggcgcctt
      901 gcgggcgcga cgctcgtcgt gacgctggag ccgtgcgcga tgtgcgcggg ggcggccctc
      961 gccgcccgcg tggcccgcgt cgtcttcggc gcgtgggacg ccaaggcggg cgcggccggc
     1021 agcgtctacg acctgccgcg cgacgctcgc ctgccggttc agatggaggt gtacggcggt
     1081 gtcgacgaag cgtcgtgcgc ccgccttctg acagagttct tcgaggcccg ccgtggccca
     1141 gacggccagg gatagggccg ccggggcgga actttaaaat gacctcatga tctctcgcgc
     1201 gaaccccgga tcccccgtac ctgtcggccc cgccgcctcc ggcgcccacg ccgatgccgg
     1261 cacggatgcc ccggccggcg gccgcccggc tgctggtgcg gatcggccct tggtcgtcgc
     1321 catcctgggc acgggttcga tgggcacggc agtgctccgc ggcctgaccg ggcccgaggc
     1381 cgggcctgcc gagatccgcg tgaccacccg cagcgcggcg catgcggcgg cattgggcgg
     1441 ccaggatggg gtcacgtcgt atgccgtcga gagcgacccc gacgcgaatc gcctggcggc
     1501 acgcggcgcc gacctcgtcg ttcttgccgt caaacctcac ctgatccccg ggcttgccgg
     1561 tgagatcggc gacgtcatcg acgcccacac gatcgtcgtc agcgtcgcgg cgggcgtcgc
     1621 gatcgccacg atcgcagggc ggctgccggc cggcgtcgcc gttctgcggg ccatgcccaa
     1681 cacgcccgcc gtgatcggcc gcggcgtgac ggggctgagc gtcacgggcg gccgaggctc
     1741 cgctgccgcc ctgcgtgtcg tgcacgacct tttcgcccgg gtgggcgagg tcgtcgaggt
     1801 ccccgaagac cggctcgacg ccctgagcgc cgtctcaggt tcggggccgg cgtacgtctt
     1861 tctccttatc gagaagctga cggcggcagc cgtgcggctc ggtttcgccg agcgggaggc
     1921 ggcgcgcctc gtcgcgggca cgttccgcgg cgccgccgag ttgctggccg cgggggaggc
     1981 cgcgcccgcc gagcttcgcc gccaggtcac gagcccgcac ggcaccacgg aacgggccat
     2041 cgcggtctta gaagaggccg ggttggacga tctcttcggc cgtgcgctgg aggctgcgct
     2101 cgcccgcgcg cgggagctgg cggcggaggg tgcctcgcgc tccgcccccg aagcctaacg
     2161 cctctggcgc cccggcttgc ggccctgggc ccgggggtct tctcgcgcct ccgacgtggc
     2221 ggctttcccg accccgcgtc aaggccgccg gcgttctggc gccgtgcctc cgacgtggcg
     2281 gctctccggg cctcgtcccg aggtcgcggg cgttcgcctg tcagtggtcg agagaggcga
     2341 aatgctcgat gtccgccgac tcgcccgaga cgatgatgag gtcgtggtcg gagatggtcg
     2401 tctcgggcgc cgcgtgcgtg tagggcttgc cgggcgactt cacaccgacg atcgtcacgt
     2461 ggtgcttgtg ccgcacgttc gcctcggcga gcgtcaggcc gcggatcgag cgcggcggat
     2521 acatcttcac gagcgcgaag tcgtcgtcga actcgatgaa gtcgagcatg cgacccgaca
     2581 cgaggtgtgc cacgcgctcg ccggcctctg cctccgggta gatgacgtga ctggcgccga
     2641 tgcgctccag gatcttgccg tgcgactggc tgatcgcctt cgtccagatc tgcggaatct
     2701 tgaggtcgac gaggttcgcg gtgatgagca cgctcgcctc gatcgaggag cccaccgcga
     2761 cgaccgcgat ggagaactcg cccgcaccga tctgcttcaa ggcgtcgata ttgcgggcgt
     2821 cggcttgcac ggtgtgggtg acgcgatccg accacttctg cacgagggcg gaatcgatgt
     2881 cgagcgccaa gacctcgcgg tcgagccggt cgagctggcc cgccgtcgcg gcgccgaaac
     2941 gaccgaggcc gatgacgagc acgggggcgt cgtgtctgat gcggtcaacc aacgatcggc
     3001 ctttcctcag ggtggtggaa cagctgccgg cgttggcttt gcgccagcgc tgcagccagg
     3061 gtcacggtgc cgacccggcc catgaacatt gtgcctgaca ggatgtacga acctagcggc
     3121 ggcagatgct gggtgatccc cgtcgacaag ccggtcgtcg cgaacgcgct gatcgactcg
     3181 aagagcacgt cgtcgagcga ctggccggtg atgttcaaga gcaggatcgt cgacacggcg
     3241 atggtcgtcg ccccccagag gacgacgctc actgcgaggc ggagcacgtc gtgcgggatc
     3301 gtgcggcgga atgccgacat cgtgtcgtcg cccttcgcct ccgcgactgc cgcgaggaac
     3361 aagacggcca gtgtcgtgac tttgatgccg cccgccgtgg aggccgagcc gccgccgatg
     3421 aacatcagca tgtcggtgat cagcaggctc gaattgttga ggtggccgac gtcgatcgtc
     3481 gcgaacccgc ccgagcgggt catggcagag aggaagaacg attggtagat ggcgtcgccg
     3541 gcgccgagcc ccgcgagggt gtgcgggttg tcgaactcca agacgaaata caagacgccg
     3601 cccgcgacga agaggatcgc gaaggcgaac agcgtcaggc gcacgtgcac cgaccagtgc
     3661 ttcttgcggg cccgcaggtt gcgtgcgaac gccagcagca ccgggaaccc gatggcgccg
     3721 aggaacactc ccgccatcag caccgtgaga aaccagtagt ccgtcgcgta gggggtaagg
     3781 ccctcggggt cgggtgtgaa gccggtgttc gtgaacgcca tcgccgagaa gtagatgctg
     3841 ttgaagatcg cctcggtgac ggaacgcccg tgcgcaagga tgcgcgggaa gagcagcacc
     3901 gccaccgcgg cctcgatgac ggccgcgctg atcgcgaccg tggcgagaag attgcccacc
     3961 tcgccgagcc ggacctcttg gccctcggcc acggggccgc ggtgcaggcg cagcgggttg
     4021 ccctcgctgg cggccaggag cttggcgcgc agcccgagcc ggcgggagat caccgtgccc
     4081 agcatggtgg cgatcgtcag tacgccgaga cctccgatct gcacgccgac gtagatgatg
     4141 acgtggccga agagcgacca gtgggtggcc atgtcgacgg tggaaagccc cgtcacacag
     4201 acgacggaaa cggccgagaa cagcgcgtcg ctgaggggcg tcaccgtgtg cgtggcggag
     4261 gcggccggca gcgagagcag cacggtgaac acgaggatca gcagggtgaa gacgaccacc
     4321 gcgaaccgtg ccgggctgcg gcccgtcacg tgactcagcc agccgcgcag cccgccgggc
     4381 cgaccgacgc gaggctgacg gggcgaaccc gtgcggggca tggcacacct ctcctgcgtc
     4441 gacccttgct gtgacccagc cagtcggggt gtcatcgtac tctgccggcc ggcgcgcccc
     4501 atgcgttgtc gtggccccga acgcgtcccc ggccccggcg aggcgtgcgg gccgaagggc
     4561 acggctgggt cgctaacctg gagccatggt cgacatcttt gccgttgtgg cagacgggac
     4621 caggcgggag atcctgcggg ttctccgcga acgggcggag cgtggcgagg cggaaagcag
     4681 cgtgtcgcag atcgtcgccg acctcggcct cagccagccg accgtgtcga agcatctgaa
     4741 ggttttgcgc gagtcgggcc tcgtgagcgt gcgggagcaa ggccagcgcc gcttctaccg
     4801 catcgacccg cgccctttgg cagaggtcgc gtcgtggctg gagcatctgc gcggcccggc
     4861 ggccggcgct caggcggtgc cggagccggc cccggccggc cgggcagtgc gggccgaggc
     4921 cacgagagcg ggcgacgccg atcgggaggt cacggagccg gccgcggtcg acaccgtagc
     4981 cttggcaggc ggccccgacc gtgccgccct cgggccacac acccgggagt tcgccggcgc
     5041 gctcggccgt tccctcgccg gcgccggcca ccgcgtgggc gatgccgcca ggcgggtgtg
     5101 gcctgcccgg cgtgccggtc gttgaccggc gcccggccgc gagcggccgg cagggcatgc
     5161 ggtaagcttg agcgctgtgc ctgccgcggc tcccgcggca gcccctgttc tgtgtgaggt
     5221 tttcgtgggt tctgtcatca aaaagcgccg caagcgcatg tcgaagaaga agcatcgcaa
     5281 gctgctgcgc aagacgcggc accagcgccg caacaagaag tagacacgaa aaagacgaac
     5341 gcgcccggcg tcacggtgac gttgatcggc aagccgggct gtcatctctg tgacgacgcc
     5401 cgtgcggcgg tgaccgaggt cgtgggcggg cttgaggcga cgcccgggtc tcccgccgtg
     5461 acattcgaag aggtctcgat t
//
LOCUS       DASTNL010000074         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_54871, whole genome shotgun sequence.
ACCESSION   DASTNL010000074 DASTNL010000000
VERSION     DASTNL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54871"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VFQ96_02560"
     CDS             complement(<1..1170)
                     /locus_tag="VFQ96_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256701.1"
                     /translation="MTKTVRRLASLLAAGALAAALLVAGQIGQAPPAQALSGSSFNAG
                     NLISNSVFTNTGSMTTAQVQSFLNAKGAACTTTSTHTCLKDYTQTTTTPSDQNQHCAP
                     YSPAKNETAAAIIVKVAKACNVNPEVILVTLQKEQGLVTSTAPTTTAYKIAMGFGCPD
                     TAPCNTEYYGFFNQVFRAARQFDVYRTNPNIFNYAAGRTSNVPYNPKSSCGSAPVTMA
                     NWATAALYDYTPYQPNAAALANLGGTGDSCSSYGNRNFWVYFTTWFGSTQVQETTVNV
                     TVTSTAGQPLSGVAVAFYKAGTTTASVTKPASTATTAADGTATVVLQKGTYKAAFVAA
                     SGTTITGPPGPSDLPSMTTWAGGTTSAADATVITAGTAIRQSLPVTIATWQAPAKS"
     gene            1506..2480
                     /locus_tag="VFQ96_02565"
     CDS             1506..2480
                     /locus_tag="VFQ96_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0256702.1"
                     /translation="MLTDVPSSGKASDVDRWAAGPGPVVAGLRDALGGHASHLSGDAA
                     VVFTRVPRTAVGLVVGVALGLAGTVMQGVTRNPLADPGILGVNGGAAFGVVLALYAFG
                     VNGISGYLWFALAGAAAASVLVYAIGSLGASGATPLKLTLAGAALSAGLGSLVNAYLI
                     ISQQTFDSFRFWQVGALAGRNWGVIAPVLPILLAGAALALATGPTLNGLALGDDAARS
                     LGQRVGWSRAVSALGVTLLCGGATALAGPIAFVGLVVPHAVRAVTGPDYRWILPGAAL
                     AAPSLVLIADVVGRVILPPGEVPAGIVTALIGGPVFIWLVRRGKKAAL"
     gene            2624..>3257
                     /locus_tag="VFQ96_02570"
     CDS             2624..>3257
                     /locus_tag="VFQ96_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU0256703.1"
                     /translation="MVPILAVAAAAAFLACCLIGQYAVSLPDFFRVLAGADLPGTSFI
                     VLDDRLPKAATAVLVGGCLGVSGTLFQTMLHNPLASPDIIGISFGASASAVVAIVVFG
                     LSGLPVSIVAGLGALGVAALIHTLSRGSLVYDHRLILIGIGVAAVLQAIVSFLLTRAD
                     ISTASDALLWLTGSLDNSTWGGVAQVGVAAAVLLPVAWALPRPLGILGLGD"
BASE COUNT          415 a         1168 c         1124 g          550 t
ORIGIN      
        1 cgacttcgcg ggggcctgcc aggtggcgat cgtcaccggc aacgattgac ggatcgccgt
       61 accggccgtg atcacggtcg cgtcggccgc gctcgtcgta ccgcctgccc acgtggtcat
      121 cgacggcagg tcagagggcc cgggcgggcc ggtgatggtc gtgccgctcg ccgccacgaa
      181 cgccgcctta tacgtgccct tctgcagcac caccgtggcc gtgccgtcgg ccgccgtcgt
      241 cgcggtgctc gccggtttcg tcaccgaggc ggtcgtcgtg ccggccttgt agaacgcgac
      301 cgcgaccccg gaaagcggct ggccggccgt gctcgtgacc gtcacattca cggtcgtctc
      361 ttgcacctgg gtggagccga accacgtcgt gaaatacacc cagaagttgc gattgccgta
      421 cgaggagcag gagtcgcccg tgccgcccaa gttggcgagg gcggccgcgt tgggctggta
      481 cggcgtgtag tcgtagagcg ccgcggtcgc ccagttcgcc atcgtcaccg gggcggagcc
      541 gcacgacgac ttcgggttgt acggcacgtt gctcgtgcgg ccggccgcat agttgaagat
      601 gttcgggttc gtgcgataca cgtcgaactg gcgggccgcg cggaagacct ggttgaagaa
      661 cccgtaatac tcggtgttgc atggtgccgt gtcggggcag ccgaagccca tcgcaatctt
      721 gtaggcggtc gtcgtcggcg cggtcgaggt gacgaggccc tgctccttct gcagcgtgac
      781 gaggatcacc tcgggattca cgttgcaggc cttcgccacc ttgacgatga tcgccgccgc
      841 cgtctcattc ttggcgggac tgtacggcgc gcaatgctgg ttctgatcgg agggggtcgt
      901 cgtggtctgc gtgtagtcct tgaggcaggt gtgggtggag gtcgtcgtgc aggcggcgcc
      961 cttcgcgttc agaaaagatt gcacctgcgc ggtcgtcatc gacccagtgt tcgtgaaaac
     1021 gctgttgctg atgaggtttc cggcgttgaa gctgctgccg gaaagagcct gtgcgggcgg
     1081 cgcctggccg atctggcccg ccacgagaag cgccgcagcc aacgctccgg ccgccaggag
     1141 cgacgcgagt cgccgcacag tcttcgtcac ggtgtgcatg ggtacgccgt tcctccatct
     1201 ggtcttgcag gcatgcccgc gccggtgacg gcgcgccgag gcggcacgca gatcatttcg
     1261 ccgagcccta tcggcacgag tcgcgcggcc gacagagaaa agactgtctc atgctacgcg
     1321 cgcgtgtcaa tcgatcttgt gcgacacaac gccacacagc caagacggcc acacgcgtgg
     1381 cggctcccgc gcccttggca ggccaaccga ccagcggggc acaaaagaca ttggctccgg
     1441 acgagccgga gccaatgcaa ctgattcacg aggcaatcgg ctaagaagcc tgcgcgatcg
     1501 gctccatgct aaccgatgtc ccgtcaagcg gcaaagcgag cgatgttgac cggtgggcag
     1561 cagggcccgg cccggtcgtc gcaggcctgc gcgacgcgct cggcgggcac gcgagccacc
     1621 tcagcggcga cgccgccgtg gtcttcaccc gggttccccg caccgccgtc ggcctcgtcg
     1681 tcggcgtcgc gttgggcctc gccggcaccg tgatgcaggg cgtcacacgc aaccccctgg
     1741 ccgacccggg aatcctcggc gtcaacggtg gcgcggcgtt cggcgtggtg ctcgccctct
     1801 acgcgttcgg cgtgaacggc atcagcggct acctgtggtt cgcgctggcc ggcgccgccg
     1861 cggcatccgt gctcgtctac gcgatcggct ccctcggcgc ctccggggcg acgccgctga
     1921 agctcacgct ggcgggcgcc gccctctccg cgggcctcgg ctcactcgtc aacgcttacc
     1981 tcatcatcag ccagcagaca ttcgactcct tccgcttctg gcaggtcggc gcgctggccg
     2041 gccgaaactg gggcgtcata gcccccgtgc tgccgatcct cctggccggc gcggccctgg
     2101 cgctcgccac cggccccacg ctgaacgggc tcgccctcgg cgacgacgcg gcccgcagcc
     2161 tcggccagcg ggtcggctgg agccgcgcgg tctcggccct cggcgtgacc ctgctctgcg
     2221 gcggggcgac ggccctggcc gggccgatcg cgttcgtcgg cctcgtcgtg ccacacgccg
     2281 tccgcgccgt caccggcccc gactaccggt ggatcctgcc cggcgcagcc cttgccgcgc
     2341 cgagcctcgt cttgatcgcc gacgtcgtcg gccgcgtcat cctgccgccg ggcgaggtgc
     2401 cggccggcat cgtcaccgcc ctcatcggcg ggccggtgtt catctggctg gtgcggcgcg
     2461 gcaagaaggc cgcgctgtga acgacatcgc gacaacgccc tcgccgcgga gcatgcgcac
     2521 cccgcaggcc gctgtccggc cctcgcgggg cgacgggcgg gcatgcccgc ctcggcggcg
     2581 tcgcgcgggg ggaaggcgca gccacggtgg cacccgcctg cccgtggtcc cgatcctcgc
     2641 ggtcgccgcc gcggcggcct tcctcgcctg ctgcctcatc gggcagtacg ccgtctcact
     2701 gccagacttc ttccgggtgt tggcgggcgc cgatctgccc ggcacctcgt tcatcgtgct
     2761 cgacgatcgg ctgccgaagg cggccaccgc cgtgctcgtg ggcggttgcc tcggcgtctc
     2821 cggaaccctg ttccagacga tgctgcacaa cccgttggcg agccccgaca tcatcggcat
     2881 cagcttcggc gcgagcgcct ccgccgtcgt cgccatcgtc gtcttcgggc tctccggcct
     2941 gcccgtctcg atcgtcgcgg ggctgggcgc gctcggcgtg gcggctctca ttcacaccct
     3001 gtcccgcggc agcctcgtct acgatcaccg gctcatcttg atcgggatag gcgtggccgc
     3061 ggtgctgcag gcgatcgtgt cgttcctgct cacccgggcc gacatttcga cggcctccga
     3121 cgccctcctg tggctcaccg gatccctcga caacagcacg tggggcggcg tcgcccaggt
     3181 cggcgtcgcc gcggcggtgc tgctgccggt ggcgtgggcc ctgccgcggc cgttgggcat
     3241 cctggggctc ggcgacg
//
LOCUS       DASTNL010000075         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_980944, whole genome shotgun sequence.
ACCESSION   DASTNL010000075 DASTNL010000000
VERSION     DASTNL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980944"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1428)
                     /locus_tag="VFQ96_02575"
     CDS             complement(7..1428)
                     /locus_tag="VFQ96_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU0256704.1"
                     /translation="MTVHVLPSRPTAAVLQVETIPLDDDDPLIPLLDPARPLLWMSGA
                     EGIAGRGEAIRLEFHGEHRIADAARAWQELTAHTRVDDAVHLPGTGLVAFGAFTFAPT
                     SRVPSVLIVPSLVVGRRGGQRWLTRLRACPPEQGAEPGTAGAQGTAPGVPPHGGAARL
                     GELLGRARQALLNGGAAGIQGPGQARHGTVPRLPQPAPAGAEYRITFTPGAMSADRYR
                     AAVAAGVARIGRGDLTKVVLARDVVGHLPIGADLRLALHTLSTSYPQCWTYAVDGLLG
                     SSPETLITVRDGAFSARVLAGTTSRGGDAAADHTHSRDLQTNGKDHREHELAVQSVVS
                     TLQAHSNDLSRSPEPYALKLPNLWHLATDVHGTLADGSTSLDLVAALHPTAAVAGTPA
                     AAASEAIDELEPFDRGRYAGPVGWLGADGDGEWAIALRGAQIDEERTITAYAGCGIVA
                     GSDPESELKESAMKLRPIVETFG"
     gene            1494..2267
                     /locus_tag="VFQ96_02580"
     CDS             1494..2267
                     /locus_tag="VFQ96_02580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0256705.1"
                     /translation="MSKADLTKQPDQVAAMFDDVGARYDRTNAILSAGNAALWRIATT
                     RAVRPRAGERILDVAAGTGTSSVALTRTGAHVVAADFSAGMLDVGRQRHGDDPRIEFV
                     AADATDLPFKTGEFDAVTISFGLRNVVDVPAALAEFLRVTRPGGRLVVCEFSTPPNPF
                     LRRLYHAYLRYGVPVVARLVSSDAPAYEYLMESIHAWPGQARLAAMLRDAGYEEVGYR
                     NLSAGIVALHRGRKPVQAAQAGAVRAAGRAKKEPAAVTA"
     gene            2264..>2447
                     /locus_tag="VFQ96_02585"
     CDS             2264..>2447
                     /locus_tag="VFQ96_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256706.1"
                     /translation="MSAPRGTLESRRRTHAARRASLAGQLGLSEKVFATASERRFART
                     VEEGLDRVEAGLRDEVR"
BASE COUNT          315 a          886 c          913 g          333 t
ORIGIN      
        1 ggccggtcag ccgaaggtct cgacgatagg gcgcagcttc atggccgatt ccttcagctc
       61 cgactcgggg tcggagccgg cgactatgcc gcacccggca tacgccgtga tggtgcgctc
      121 ttcgtcgatc tgcgcgcccc gcagggcgat cgcccactcg ccgtcgccgt cggcgccgag
      181 ccagccgacg ggccccgcgt accggccgcg gtcgaacggc tccagctcgt cgatcgcctc
      241 gctcgccgct gccgccgggg tgccggccac cgcggccgtc gggtgcaggg ccgccaccag
      301 gtcgaggctc gtcgagccgt cggcgagcgt gccgtgcaca tcggtggcga ggtgccacag
      361 gttcggcagc ttgagggcgt aaggctccgg cgagcgggag aggtcgttgc tgtgcgcctg
      421 cagtgtggag acgacgctct gcaccgccag ctcgtgctcg cggtggtcct tgccgttcgt
      481 ctgcaggtcg cgggagtggg tgtggtcggc cgccgcatcg ccgccccgcg aggtcgtgcc
      541 cgccagcacg cgggcggaga aggcgccgtc acgcacggtg atgagcgtct ccgggctgga
      601 gcccagcagg ccgtccaccg cgtaggtcca gcattgcggg taactggtcg acagcgtgtg
      661 cagcgcgagg cgcaggtcgg cgccgatcgg caggtggccg acgacatcgc gggccagcac
      721 gaccttcgtc agatcgcctc gcccgatgcg ggccaccccc gccgcgacgg ccgcgcggta
      781 tcggtcggcc gacatcgcgc cgggggtgaa cgtgatgcgg tattcggccc ctgccggcgc
      841 cggctgcggc agccggggca cggtgccgtg ccgtgcctgg ccgggcccct ggatgccggc
      901 cgcgccgccg ttgagcaggg cctgcctcgc ccggccgaga agctcgccga gccgcgccgc
      961 cccgccgtgt gggggaacgc cgggtgccgt gccctgcgca ccggccgtgc ccggctcggc
     1021 gccctgctcg ggcgggcagg cgcgcagccg ggtaagccat cgctgcccgc cccggcgacc
     1081 gacgacgagc gaggggacga tcaagaccga ggggacacgg ctcgtcggcg cgaaggtgaa
     1141 agcgccgaac gcgacgaggc cggtgcccgg caggtgtacc gcatcgtcta cccgcgtgtg
     1201 cgcggtcagc tcctgccagg cgcgcgccgc gtcggcgatg cggtgctccc cgtggaattc
     1261 gaggcggatg gcctcccccc gcccggcgat gccctccgcg ccgctcatcc agaggagcgg
     1321 ccgcgcgggg tcgagcaagg ggatgagggg gtcatcgtcg tcgagcggga tggtctccac
     1381 ctggagaacc gctgccgtgg gccgggacgg caacacgtgt acggtcacca gccaagccta
     1441 ttgcgcccgg cctgcccgcg ccgcgcggcc cggcgcacgc atagacttga gaagtgagca
     1501 aggccgactt gaccaagcaa cccgatcagg tcgccgcgat gttcgacgat gtcggcgctc
     1561 gctacgatcg cacgaacgcg atcctctcgg cgggcaacgc ggcgctgtgg cgtatcgcga
     1621 cgacccgggc cgtgcgcccc cgggcggggg agcgcattct cgacgtcgcg gccggcaccg
     1681 gaacctccag cgtcgcgctc acccgcacgg gcgcccacgt ggtggcggcg gatttctcgg
     1741 ccggcatgct cgacgtcggc cgccagaggc atggcgacga cccgcgcatc gagttcgtcg
     1801 ccgccgacgc gaccgacctg ccttttaaga cgggggagtt cgacgcggtc accatctcgt
     1861 tcgggctgcg caacgtggtc gacgtgcccg ccgcgctcgc cgagttcctc cgcgtcacga
     1921 ggcccggcgg ccggcttgtc gtctgcgagt tctcgacgcc gccgaacccg tttctgcgca
     1981 ggctctatca cgcctacctc cgctacggcg tgcccgtcgt ggcacgcctc gtcagctctg
     2041 acgcgcccgc ctacgaatac ctcatggagt cgatccatgc ctggcccggg caggcgcggc
     2101 ttgccgcgat gctgcgcgac gccggctacg aagaggtcgg ctaccgcaac ctgagcgccg
     2161 ggatcgtggc gctgcatcgc ggccggaagc cggtgcaggc ggcccaggcg ggggccgtgc
     2221 gggctgccgg ccgggcgaag aaggagcccg cggcggtgac cgcgtgagtg caccccgcgg
     2281 cacgttggag tcccggcgcc gcacccacgc cgcccgacgc gcgtcgctgg ccggccagtt
     2341 gggcctcagc gagaaggtct tcgcgacggc ctcggaacgg cgattcgccc gcacggtcga
     2401 agaaggcctc gaccgggtgg aggcgggcct tcgtgacgag gtgcgct
//
LOCUS       DASTNL010000076         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_611166, whole genome shotgun sequence.
ACCESSION   DASTNL010000076 DASTNL010000000
VERSION     DASTNL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611166"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1267)
                     /locus_tag="VFQ96_02590"
     CDS             complement(5..1267)
                     /locus_tag="VFQ96_02590"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEU0256707.1"
                     /translation="MKRVAVLSVAALDALVAAAVGLAVCLIPAVVIWIAQYGMAGGAR
                     TPWQAAGDVWLVGHGVDLTVAVSTRRMAALGLPDHAGGFTVTLAAAGLGLLTALVAAR
                     VGRRSDRAGHPWAGVIGVAAVLLAVGALVTTTTPGPAVFAETWQGVLLPAAVGVVGAG
                     VGVAYGRATTKAADRRRWRLPAGLAGRALAAVRLGDVRVRSGLAAAVRGAAVAALVFA
                     ALAALALFVSLFGHYAQVVGLYEGVGSGVGGGIILTLAQVILVPNAVAWSMAWLAGPG
                     FAVGAGTVASPLGVQLGGMPALPLLGAVPRNGGSFGLVTVLVPVAAGVVAGLLVRRRT
                     GRAPYAVPTWLGAVALGIGVLCGVAVGLVLWWCSGGVGPGGLARFGPAPLPVGAAVAA
                     EVALGAALGLFSARLPDFGRVRRLERHR"
     gene            complement(1357..2262)
                     /gene="sucD"
                     /locus_tag="VFQ96_02595"
     CDS             complement(1357..2262)
                     /gene="sucD"
                     /locus_tag="VFQ96_02595"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU0256708.1"
                     /translation="MSIFLDQDSKVIVQGITGSQGSKHTALMLKAGTKIVGGVNARKA
                     GTTVQHTAADGSPVTLPVFATVREAMAQTGATVSVIFVPPAHAKEAMVEAIDAEIPLL
                     VVITEGIPVQDAADAWARAVAAGGRTRIIGPNCPGIITPGQSLVGITPASITGPGPIG
                     LVSKSGTLTYQMMYELRDLGFSTALGIGGDPVIGTTHIDALAAFEADPETKAIVMIGE
                     IGGDAEEQAARFIQEHVTKPVVGYIAGFTAPKGKTMGHAGAIVSGSAGTAQAKKEALK
                     AAGVQVGKTPSEAARLLREAVAALV"
BASE COUNT          336 a          839 c          857 g          264 t
ORIGIN      
        1 cagcctaccg gtgcctctcc aggcgacgaa cgcgcccgaa gtcgggcagc cgggccgaga
       61 agagcccgag cgccgcaccg agcgccacct ccgccgcgac cgccgcgccg accggcagcg
      121 gggcggggcc gaaccgcgcc aggccgccgg ggccgacgcc gccagagcac caccacagga
      181 cgaggccgac ggccacgccg cacagcaccc cgatgccgag ggcgacggcg cccaaccagg
      241 ttggaaccgc gtacggcgca cgccccgttc ggcgccgcac gagaaggccc gccacgaccc
      301 cggcggccac gggcacgagg accgtgacga ggccgaagct tccgccgttc ctcggcacgg
      361 cgccgagcaa gggcagggcg ggcatgccgc ccagctgcac gccgagcggg cttgcgacgg
      421 tgcccgcgcc cacggcgaag ccggggcccg ccagccacgc catcgaccac gcgacggcgt
      481 tgggcacgag gatcacctgg gccagggtga ggatgatgcc gccgccgacg ccggatccca
      541 caccttcgta gaggcctacg acctgggcat agtgcccgaa aagagagacg aagagcgcca
      601 gggccgcgag cgccgcgaag acgagggccg cgaccgcggc accccgtacg gcggccgcga
      661 ggcccgaccg cacgcggacg tcgccgagac gcacggcagc gagggcacgg ccggcgagcc
      721 cggcaggcag ccgccatcgt cggcggtcgg cagccttagt cgtggcccgg ccgtaggcga
      781 cgccgacgcc cgcccccacg accccgacgg cggcgggcag gagcacgccc tgccacgttt
      841 cggcgaagac ggccgggccg ggcgtcgtcg tggtgacgag cgcgcccacg gcaaggagca
      901 cggcggcaac cccgatgacg cccgcccacg gatgcccggc acggtcgctg cgccggccga
      961 cgcgggccgc gaccagcgcg gtgaggaggc ccagccctgc ggcggcaagc gtcacggtga
     1021 agccgccggc gtggtcgggc agcccgaggg cggccatccg gcgtgtcgac acggcgaccg
     1081 tgaggtcgac gccgtggccc acgagccaca cgtcgccggc ggcttgccac ggagtgcggg
     1141 cgccgccggc catgccgtat tgcgcgatcc agatcacgac cgcggggatc aggcacacgg
     1201 cgaggccgac cgctgccgcg accagcgcgt cgagtgcggc gacggagagg actgcgacgc
     1261 gtttcactgc agcggagcct accgtagcgc ctacccgggc cggtgccggc gcgcgggccc
     1321 agggctctca cgcccggccc gtgcgccggc accaggtcag acgagcgcgg cgacggcctc
     1381 ccgcagcagg cgtgcggcct cgctgggggt cttgccgacc tgaacgccgg ccgccttcag
     1441 cgcctctttc ttggcctgcg cggtgccggc cgagccggag acgatcgcgc cggcgtgccc
     1501 cattgtcttg cccttcggcg cggtgaatcc ggcgatgtag ccgacgacgg gcttcgtcac
     1561 gtgctcctga atgaaccggg cggcctgctc ttccgcgtcg ccgccgatct cgccgatcat
     1621 gacgatggcc ttcgtctcgg ggtcggcctc gaacgcggcg agcgcgtcga tgtgggtggt
     1681 gccgatgacc gggtcgccgc cgatgccgag ggcggtcgag aagcccaggt cgcgcagctc
     1741 gtacatcatc tggtaggtga gggtacccga cttggagacg aggccgatcg ggccgggccc
     1801 ggtgatcgag gccggcgtga tgccgacgag cgactggccg ggcgtgatga tgcccgggca
     1861 gttcgggccg atgatgcggg tgcggccgcc cgcggccacg gcgcgcgccc aggcatccgc
     1921 cgcgtcttgc accggaatgc cctcggtgat gacgacgagc agcgggatct cggcgtcgat
     1981 cgcctcgacc atcgcctctt tggcgtgggc cggcggcacg aagatgacgg acacggtggc
     2041 gcccgtctgc gccatcgcct cccgcaccgt ggcgaagacc ggcaacgtca cgggcgagcc
     2101 gtcggccgcg gtgtgctgaa cggtggtgcc ggccttgcgg gcgttcaccc cgccgacgat
     2161 cttcgtgccg gccttcagca tgagggcggt gtgcttgctg ccctggctgc cggtgatgcc
     2221 ctgaacgatg accttggagt cttggtcgag aaagattgac atgcgcgttc cttcgttctt
     2281 cgaggccggt tcagtt
//
LOCUS       DASTNL010000077         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_612102, whole genome shotgun sequence.
ACCESSION   DASTNL010000077 DASTNL010000000
VERSION     DASTNL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612102"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFQ96_02600"
     CDS             <1..343
                     /locus_tag="VFQ96_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256709.1"
                     /translation="RRWAHTLLANTPDDGSTSPLMTVLSRIGFAPEDPASPAPERTIR
                     LHACPFRDAANDYPNVVCSVHRGLVDGSLEHCGKTAKDAVLKPFVGPHLCEVEMLASD
                     TAADEKPAALE"
     gene            427..2076
                     /locus_tag="VFQ96_02605"
     CDS             427..2076
                     /locus_tag="VFQ96_02605"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU0256710.1"
                     /translation="MVFPRSGAAATLTATRPASRPATADRRRRRWAWWGSIGGLAVAA
                     FVARLLFTLHDGGFLDVHDYDAGVYYAAADALIHHQMPYQSFLFIEAPGVTLITAPFA
                     ALGSLTTDAVGLAAANLAFAAMGTASTAMVTVNLRRFGMPAALAGGFFYAVTDAVVTS
                     ERSIRLEPPATLLILISLVLLTNTRPTRRSRRAFIAGILLGAAIGFKIWYVVPALVVL
                     LVSRGRRRPILAGVVAGAFVIYLPFFLPAPTLMLRQVVLDQLGRPRDPISLASRVHGI
                     LGEPSVPGSLTRLGFTTTELSAILLVVAAAAVVVAWRVRRARLYVLLLVTGAGVLLAS
                     PSFFAYYAALTSFPLAVVLGVAFGRLVAALHAPGGMTAAASVLVMAGMLAVSLPKEIH
                     ASDPAAPLTGLRSAVARVHGCVLSDNNAILAATNVLTRDLRQGCLIWPDTTGWAFDAY
                     AVHPADHRAVSRMANPLWQAHAVGYLTSGAAVIPYYAGTLLDHRSTLAVRHGTTLFQG
                     GEWAVYATPRRLGPAPSTPALLAWAAHGGAHEAPASSRNPH"
     gene            complement(2073..>2409)
                     /locus_tag="VFQ96_02610"
     CDS             complement(2073..>2409)
                     /locus_tag="VFQ96_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256711.1"
                     /translation="PVLSMLVAACVFFGLILVAEALFRVLGPAAHAVVTRLSFAGILV
                     VLLHPLVWWIVLSFVPALPGVMIFVVCAVVPWTVGTLSLWSKPAGWLAGTGRSELRRV
                     WRAQLGRGA"
BASE COUNT          339 a          914 c          782 g          374 t
ORIGIN      
        1 gcggcgttgg gcacacaccc tgctcgcgaa cacaccggac gacggatcga cctcgccgct
       61 catgaccgtg ctctcccgca tcggcttcgc ccccgaagac ccggcaagcc cggctcccga
      121 gcgcaccatc cgtctgcacg cctgtccgtt ccgcgacgcg gcgaacgact acccgaacgt
      181 cgtctgctcc gtgcaccgtg gcctcgttga cggctccctg gagcattgcg gcaagacggc
      241 gaaagacgcg gtgctgaagc cgttcgtcgg gccgcacctg tgcgaggtcg agatgctcgc
      301 ctcagacacg gcggccgacg agaagccggc agccctcgag tagaggccca ccgcaggcag
      361 gcgccgtcgg cgcccgaccg agtgttcctt agtgctgtct gtgtgacagc tccctagcgt
      421 cgaggcatgg tcttccccag gtcgggcgcc gcggccaccc tcacggcgac gcgcccggcc
      481 agtcgacctg ctacggccga ccggcgccgg aggcggtggg cctggtgggg ctcgatcgga
      541 gggctggctg tcgccgcgtt cgtcgcccgc cttctcttca ccctgcacga tggcgggttc
      601 ttagacgtgc acgactatga cgccggcgtc tattatgcgg ccgccgacgc cctcatccac
      661 caccagatgc cctatcagtc gttcctcttc atcgaggcgc ccggcgtgac gctcatcacc
      721 gcgccgttcg cggctcttgg tagcctcacg accgacgccg tgggcctcgc ggccgcaaac
      781 ctcgccttcg cggcgatggg caccgcctcc accgcgatgg tcaccgtgaa cctgcgtcgg
      841 ttcgggatgc cggcggccct ggccggcggg ttcttctatg ccgtcaccga cgcggtcgtc
      901 acgtcggagc gcagcattcg gctggagccg cccgcgacac tcctgatcct gatctcgctc
      961 gttctgctca cgaacacccg cccgacgcga cgcagccggc gtgcattcat cgccggaatc
     1021 ctcctcggcg ccgcgatcgg attcaagatc tggtacgtcg ttcccgccct cgtcgtcctt
     1081 ctggtgtcgc gcggccggcg acgcccgatc ctcgccgggg tcgtggccgg cgccttcgtg
     1141 atctacctgc ccttctttct cccggcgccc acgctgatgc tccggcaggt cgtcctcgac
     1201 cagctcggca ggccgcgaga tcccatctcc ctcgcctccc gcgtgcacgg aatcctcggc
     1261 gaacccagcg tgccggggag cctcacacgg ctcggcttca cgacgacgga gctctccgcc
     1321 atactgctcg tcgttgccgc ggcggccgtc gtcgtcgcct ggcgggtgcg cagggcccga
     1381 ctctacgtgc tcctgctcgt gacgggcgcc ggggtgctcc tggcgtcgcc gtcgttcttc
     1441 gcgtattacg ccgccttgac ctcgttcccg ctcgccgtcg tgctcggcgt cgcgttcggg
     1501 cggctcgtcg cggcgctgca tgcccccggc ggcatgacgg ccgcggcgag cgtgctcgtc
     1561 atggcaggaa tgctcgccgt cagcctgccc aaggagatcc atgcgagcga ccctgcggca
     1621 ccgctgaccg ggttgcgctc cgccgtcgcg cgggtgcacg gctgtgtcct ctccgacaac
     1681 aacgcgatcc tcgcggccac gaacgtgctc acccgagacc tccggcaggg ctgccttatc
     1741 tggccggata cgacagggtg ggccttcgac gcctacgccg tgcatcccgc cgatcaccgg
     1801 gcggtgtccc ggatggcgaa cccgctctgg caggcgcacg cggtcgggta cctcacctcg
     1861 ggcgccgctg tcatcccgta ctacgccggc acgctgctcg accaccgttc caccctcgcc
     1921 gtgcgccacg gcacgacact cttccagggg ggcgagtggg cggtgtacgc cacgccgcgt
     1981 cgcctcggcc cggcgccttc cacgcccgcc ctcctggcct gggcggcaca cggtggggca
     2041 catgaggctc ctgccagcag tcggaacccc cactaggcac cgcggccgag ctgggctcgc
     2101 cagacgcggc ggagctccga gcggcccgtt cccgcgagcc agccggcagg cttactccac
     2161 aacgacaggg tgcccaccgt ccacggcacc acggcgcaga cgacaaagat catgacgccc
     2221 gggagcgcag gcacgaacga caacacgatc caccagacga gcgggtgcag gagaaccacg
     2281 aggatgcccg cgaacgacag gcgggtgacg acggcatggg cggccgggcc gaggacgcgg
     2341 aagagtgcct cggcgacgag gataaggccg aagaaaacac acgcggcgac gagcatcgag
     2401 aggaccggc
//
LOCUS       DASTNL010000078         6086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_982721, whole genome shotgun sequence.
ACCESSION   DASTNL010000078 DASTNL010000000
VERSION     DASTNL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982721"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFQ96_02615"
     CDS             <1..762
                     /locus_tag="VFQ96_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0256712.1"
                     /translation="KPRDEYLLSTKVGRLLRPDPAGRGRLDTEHDFWVDAGTKRVWDF
                     TAAGLRRSLEESLTRLGVDRVDTVFLHDPEKYDLGLADREAYPALAAMRDEGLVGRVG
                     VGSMSVEALARAARHSELDVMMIAGRLTLAEQPVLPEVLESARRHGLGVVAAGVFNSG
                     LLSHAAPRADSRYEYGATPAALLAHVRQIAAVCEEHGVDLPTAALQYPMTIDVVESVV
                     VGGSRPRHVLQNVARMSAPVPPGLWSDLRERGLIA"
     gene            759..1634
                     /locus_tag="VFQ96_02620"
     CDS             759..1634
                     /locus_tag="VFQ96_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0256713.1"
                     /translation="MIAPTIDAHLHLWRKRPGRYRWLEKADPALQRDVTATDAQAALA
                     TCGVRQAILVQAEDTIADTEEMLAAAGRAPWVVGIVGWVRLDSAEADGQLENWARHRL
                     IRGIRHLVHDDPRDDFLDLPQVRRSLRRVASRGFVFEVPDAWPRHLGAVGRLAAALPD
                     LTVVVDHLAKPPLGEEAYGAWRAALYDVARRDNTVAKVSGLYDTPGPLSGGALRRVWD
                     DALDMFGPDRLLWGGDWPMSIPHGGYRPTWEALRPLVAELSVGEQEALLGGTARQLYR
                     LDDDPPGTGLGTAGT"
     gene            1666..2088
                     /locus_tag="VFQ96_02625"
     CDS             1666..2088
                     /locus_tag="VFQ96_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HEU0256714.1"
                     /translation="MLKGLDPLLTGQLLQILDEMGHSDTIVVTDAHFPAASLATRLIA
                     LPGVGSPEVVRAICSVLPLDQAPTLALMTSASGEVLPVHSELMAAAGVAQRDTRFVDR
                     FAYYDEAKAAFAIVRTGERRTYANALLSKGVVATSAEG"
     gene            2255..3118
                     /locus_tag="VFQ96_02630"
     CDS             2255..3118
                     /locus_tag="VFQ96_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0256715.1"
                     /translation="MRLGRIGERGREIPVAAVGEAVYDLTPVTRDIDGEFLAAGGIDL
                     VRERIAAGALPRLADALELRVGAPVARPQAVYCIGMNYAAHAAESGNKPPENMVMFLK
                     SPNTVSGPDDDVEIPFSSVKTDWEVELAVVIGRRAWRLGSEADVASYVAGYTVGNDLS
                     ERDWQIAVSGGQWSKGKSAPGFLPLGPWLVTPDEIDPTGVRLRSWVNGQVRQDSNTAD
                     MIFPVDHVVWELSQYLVLEPGDVILTGTPQGVALSGRFPYLKAGDVVELEIDGIGRQR
                     QAYVPAGQGRR"
     gene            3122..3874
                     /locus_tag="VFQ96_02635"
     CDS             3122..3874
                     /locus_tag="VFQ96_02635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0256716.1"
                     /translation="MGEFDGLVAVVTGGASGIGAAIAVRLRHDGARVAVFDVNPEAAD
                     ADLAVAVDVTDDASVRAGVDRVAAELGGIDILVNNAGIGAQGDVAANTDEEWHRVWDV
                     NVVGMVRVARAALPHLRRSAHAAVCNTSSIAATAGLPQRVLYSATKGAVLAMTRAMAA
                     DHLREGIRVNAVNPGTADTPWIGRLLSKAPDPAAERAALAARQPHGRLVDPAEIADAV
                     AYLVSPSSRSTTGVGLAVDGGMQDLRLRPQQS"
     gene            3975..4625
                     /locus_tag="VFQ96_02640"
     CDS             3975..4625
                     /locus_tag="VFQ96_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEU0256717.1"
                     /translation="MLRLYADSADTDLFADLLSSGVLVGVTTNPTILQAAGRSLADSA
                     ALFERWHALGAREVFFQAVGASEDDYLDSARRICAIDPSVSVKIPATGEGFRAAARLA
                     RGGASVLVTAVYTPAQAAVASAVGAKYIAPYLGRLDDSGRDGLAVIRSMMAAMRGSDT
                     EVLAASVRTPRALAGLAEAGVRHITAKPAMLRQSFESASTRAAVADFDAATAASAE"
     gene            complement(4727..4990)
                     /locus_tag="VFQ96_02645"
     CDS             complement(4727..4990)
                     /locus_tag="VFQ96_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0256718.1"
                     /translation="MTWVIRTDRDFDRALKKLDRQVAARVLKALIALEDLDDPTKRCK
                     ALSGPYAGLWRLRVGDYRVILDIRRAELIIIALDLGNRSTVYG"
     gene            complement(4987..5289)
                     /locus_tag="VFQ96_02650"
     CDS             complement(4987..5289)
                     /locus_tag="VFQ96_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEU0256719.1"
                     /translation="MEVRCGSERVAVKEVPRSLRLPVEVDERLSRLAAATGRSKSYYL
                     RELVTTGIDDLEYAYGLAERAEAIRAGRRTTRPLDDLLSEMSISRDDLAAMPDQSA"
     gene            5534..>6086
                     /locus_tag="VFQ96_02655"
     CDS             5534..>6086
                     /locus_tag="VFQ96_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256720.1"
                     /translation="MPNRPDYVPDPRRWRVLGVLSAALFMALIDVSIVNVALPSIQHG
                     LDATESDLQWVLSGYALTFGIGLVTAGRAGDLYGRGGYFLIGVVVFTASSAWAGLAPD
                     ALWLNIARAVEGIGSGLISPQVIGMIQQYFRGAERGRAFGIFGGVVGVSVAIGPVLGG
                     LLIQLGGPVDGWRWVFFVNIPVGV"
BASE COUNT          883 a         2018 c         2195 g          990 t
ORIGIN      
        1 aagcctcgtg acgagtatct gctctcgacg aaggtcggcc ggctgttgcg gcccgacccc
       61 gccggccggg ggcggctcga caccgagcac gacttctggg tcgacgccgg cacgaagagg
      121 gtctgggact tcacggccgc cggcctgcgg cgcagccttg aggagtcgct gacccgtctg
      181 ggtgtcgacc gcgtcgacac ggtctttctt cacgatccgg aaaagtacga cctcggcctg
      241 gctgaccgag aggcctatcc tgctctcgcc gccatgcgcg acgaagggct cgtcggccgc
      301 gtcggtgtgg gctcgatgtc tgtcgaggcg ctcgcccgcg cggcccggca ctccgaactg
      361 gatgtcatga tgatcgccgg ccggctcacc ctggcggaac agcctgtcct gcccgaggtg
      421 ctggaatccg cgcggcgcca cgggctgggg gtcgttgcgg ccggcgtctt caattcgggc
      481 ctgctctcgc acgccgcacc tcgcgccgat tcccgctacg aatacggcgc gacgcccgca
      541 gcacttctcg ctcacgtgcg gcagatcgcc gcggtgtgcg aggagcacgg cgtcgatctg
      601 ccgactgccg cgctccagta cccgatgacg atcgacgtcg tcgaatccgt cgtggtcggc
      661 gggtcgcggc cgaggcacgt gctgcaaaac gtggcaagga tgtcggcgcc cgtgccgccg
      721 ggcctctgga gcgacctgcg cgaacggggg ctgatcgcgt gatcgcgccg acgatcgacg
      781 cccacctgca tctgtggcgc aagcggcccg gccgctaccg atggctcgag aaggccgacc
      841 ccgctctgca gcgcgacgtg acggcgaccg acgcgcaggc ggccctcgcc acctgcggcg
      901 tccgacaggc gatcctcgtg caggccgagg acacgatcgc agacaccgag gagatgctgg
      961 cggcggccgg ccgggcgccg tgggtggtcg gcatcgtggg ttgggtccgt ctcgattcgg
     1021 ccgaagccga cgggcagctg gagaactggg cacggcatcg cctcatccgc ggcatccgcc
     1081 accttgtcca cgacgatccg cgagacgatt ttctcgacct gccgcaggtg cgccgctccc
     1141 tgcgtcgcgt ggcgtcgcgg gggttcgtct tcgaggtgcc agacgcttgg ccgcggcacc
     1201 tcggtgcggt gggccggctc gccgccgcat tgcccgacct gaccgtcgtc gtcgaccacc
     1261 tggccaagcc tcccctcggc gaggaggcgt acggcgcctg gcgggccgcc ctctacgacg
     1321 tggctcgccg cgacaacacc gtcgcgaaag tctctgggtt gtatgacaca cctgggccgc
     1381 tgagcggcgg tgcactgcgc cgcgtgtggg acgacgcgct cgacatgttc gggccggacc
     1441 gccttctctg gggcggcgac tggccgatga gcatccccca cggcggctac cggccgacct
     1501 gggaggcgct gcggcccctc gtggccgagc tttccgtcgg cgaacaggag gccctgctcg
     1561 gcgggacggc tcgacagctg taccgactgg acgacgatcc gccgggcacc ggcttgggaa
     1621 cggccggcac gtgacgtatc gaacgatggg gcaaggggag acatcatgtt gaagggtttg
     1681 gacccgcttc tgacagggca gttgctgcag attctcgacg agatggggca ttccgacacg
     1741 atcgttgtca ccgacgcaca ttttccggcg gcgagtctcg cgacgcggct catagccctg
     1801 ccgggagtgg ggtcgcccga ggtcgtgcgg gcgatctgca gcgtcttgcc gctcgaccag
     1861 gcacccacct tggctctcat gacctcggcc tccggtgagg tgctaccggt gcatagcgag
     1921 ttgatggcgg ctgccggcgt ggcgcagcgg gacacgcggt tcgtcgaccg cttcgcgtat
     1981 tacgacgagg cgaaggctgc cttcgccatc gtgcgcacag gagagaggcg cacctatgcg
     2041 aacgccttgc tgagcaaggg ggtcgtcgcg acgtcggctg aaggttgagc cgggtggccg
     2101 gccccgatac cggggtcggg aggtacgagg tacgcgagca ccggtcatag ggccggctcg
     2161 ggcaggcagg accaagccga gcacgcgaga acccgccggc acaccgggcc ggacgagaga
     2221 ctacgagaac gagcgaggag acaggagaca acggatgcgt ttgggacgaa tcggtgagag
     2281 gggccgggag attcccgtcg cggcggtcgg agaggccgtc tacgacctca ccccggtcac
     2341 tcgcgacata gacggtgagt ttctcgccgc cggcggcata gacctcgtgc gggagcgaat
     2401 cgcggccggc gcgttgccgc gcctggctga cgccttggaa ctgcgcgtcg gggcgccggt
     2461 ggcccgcccc caggcggtgt actgcatcgg catgaactac gccgcgcacg ccgccgaatc
     2521 cggcaacaag ccgcccgaga acatggtgat gttcctgaaa tcgccgaaca cggtctcggg
     2581 gccagacgac gacgtggaga tacccttcag cagcgtcaaa acggattggg aggtcgagct
     2641 cgccgtcgtg atcggccgcc gtgcctggcg cctggggagc gaggccgacg tggcgtcgta
     2701 cgtcgccggc tacacggtcg gcaacgacct ctcggaacga gattggcaga tcgccgtctc
     2761 cggcgggcag tggtcgaagg gcaagtcggc gccgggtttt ctccctctgg ggccgtggct
     2821 cgtcacgccc gacgagatcg acccgaccgg ggtgcgtctg cgttcctggg tcaacgggca
     2881 ggtgcggcag gattcgaaca cggccgacat gatcttcccc gtcgaccacg tcgtgtggga
     2941 gctgagccag tacttggtgc tggagccggg ggacgtcata ctcaccggga cgccgcaggg
     3001 ggtggccctt tctggccggt ttccctacct gaaggccggc gatgtggtcg aactggagat
     3061 cgacggcatc ggccggcagc ggcaggccta tgtgcctgcc gggcagggga ggcgctgagt
     3121 catgggtgaa ttcgacgggc tagtcgccgt cgtcacgggc ggggcctccg gcatcggtgc
     3181 ggccatcgcc gtccggctgc gccacgacgg cgcccgggtg gcggtcttcg atgtgaaccc
     3241 ggaggcggcg gatgcggacc tcgcggtcgc cgtcgacgtc accgacgacg cctcggtgcg
     3301 cgccggcgtc gaccgggtgg ccgccgagct gggcggcatc gacattctcg tgaacaacgc
     3361 cggcatcggc gcccagggcg acgtggcggc gaacaccgac gaggaatggc atcgggtgtg
     3421 ggacgtgaac gtggtgggca tggtgcgcgt ggcgcgggcc gccttgccgc acctgcgccg
     3481 ctcggcgcat gcggccgtct gcaacacctc ctccatcgcc gcgaccgccg gcctgccgca
     3541 gcgcgtcctc tacagtgcga cgaagggggc ggtgctcgcc atgacccggg cgatggcggc
     3601 cgatcacctg cgggagggca tccgcgtgaa cgccgtcaac ccgggcaccg ccgacacccc
     3661 ctggatcggc cggctcctga gcaaggctcc agacccggcg gccgagcgcg ccgccctggc
     3721 ggcacgccag ccgcacggtc gcctcgtcga ccccgcggag atcgccgacg ccgtcgccta
     3781 cctggtcagc cccagctcac ggtcgaccac gggcgtcggc ctcgccgtcg acgggggcat
     3841 gcaagacctg cgcttgcggc cgcagcaaag ctgacgccgc cgggcttcgt ggtcgacggg
     3901 tgccgcacga gatgcccgcc gccgacgcgc gtgagtcggt cgggtgatcg ctcgcggaga
     3961 gaaggagtca ttaaatgttg agactgtacg ccgattcggc cgataccgac cttttcgccg
     4021 acttgctttc ctccggagtg ctcgtcggcg tcaccacgaa cccgaccatt ctgcaggcgg
     4081 ccgggcgctc gctcgccgac agtgctgctt tgttcgaacg ctggcacgcg ttgggcgccc
     4141 gagaggtgtt ctttcaggcg gtgggcgcgt cggaagacga ctatctcgat tcggcgcgcc
     4201 ggatttgcgc cattgatcct tcggtgagcg tcaagatccc cgcgacgggg gaggggttca
     4261 gggccgcggc ccgcctggcg cggggtggcg cctcggtgct cgtcaccgct gtctacacgc
     4321 cggctcaggc ggccgtggcc tcggccgtgg gggcgaaata tatcgccccc tacctcggcc
     4381 ggcttgacga cagtggcaga gacgggctcg cggtcatccg ctcgatgatg gcggcgatgc
     4441 ggggctccga caccgaggtc ttggcggcca gcgtacgcac gccgcgggcg cttgccgggc
     4501 tggccgaggc cggcgttcgg cacatcaccg cgaagcccgc gatgctgcgc cagtcgttcg
     4561 agagcgcgag cacgcgtgcg gcggtcgccg atttcgatgc cgccacggcg gcctcggccg
     4621 agtgaacgcg atccggggga gaatgccatg aacgcgtcga cgccggccat ttgaccggat
     4681 cacgggcgtt accactgacg ccgtccgccg gaatggcgga tgttcgtcag ccgtagacgg
     4741 tgctgcggtt gcccaggtcg agggcgatga tgatcagttc cgcccggcgg atgtcgagga
     4801 tcacgcggta gtcgcccacc cgtagccgcc agaggccggc gtaggggccg gacagggctt
     4861 tgcaccgctt cgtcggatca tccaggtcct ccagcgcgat gagtgctttc agcacgcggg
     4921 cggcgacctg ccgatctagc ttcttcagcg cccggtcaaa gtcacgatct gtgcggatga
     4981 cccatgtcat gcggattggt cgggcatcgc ggccagatca tcgcggctga tgctcatctc
     5041 ggacaggaga tcgtccagcg ggcgggtggt gcgccggccg gcccggatgg cctcggcgcg
     5101 ctcggccagc ccgtaggcat attccaggtc gtcgatcccg gtcgtgacca gctcgcgcag
     5161 gtaatagctc ttggatcggc cggtggccgc agccaatcgg gagagccgtt cgtcgacttc
     5221 caccgggagg cgaagggacc gtggaacctc tttcactgcc actctttcac tgccacaacg
     5281 cacctccatg actacatgta gtcaccagcc tatcaggagg cggcgtgcct cgtgcagggc
     5341 ccgggctgca agcgcggcgc gacagccggt catcggtgag ggactgaccg tatttctggg
     5401 cgaatgacat cggcgctggc agcgtaggac ccaagccgcg ccgcaagcct cagtcggagt
     5461 gttctctatg ccctggccgg gttgccgctc gaggtttgtc gaaaggtcgc gccgtagagt
     5521 tgatcgagtg accatgccga accgcccgga ctacgttccc gacccgcggc gatggcgtgt
     5581 tctcggcgtc ttgagcgcgg cgctgttcat ggcgttgatc gatgtgagca tcgtcaacgt
     5641 cgcgctgcca tcgatccagc acggcctcga cgcgaccgaa tccgacctgc aatgggtgct
     5701 ctccgggtac gcgttgacgt tcggcatcgg ccttgtcacc gcgggccgcg ccggcgacct
     5761 gtatgggcgc ggtggctact ttcttatcgg cgtcgtggtg ttcaccgcgt cgtccgcatg
     5821 ggcggggctg gcccccgacg ccctgtggct gaacatcgcc cgcgcggtgg agggcatcgg
     5881 ttcgggcctc atctcgccgc aggtgatcgg catgatccag cagtacttcc gcggggcgga
     5941 gcggggccgg gcgttcggca tcttcggcgg cgtcgtgggt gtctccgtgg cgatcgggcc
     6001 ggtcttgggc gggctcctca tccagctggg cgggccggtg gacggctggc gatgggtgtt
     6061 cttcgtcaac atacccgtcg gtgtcg
//
LOCUS       DASTNL010000079         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1169416, whole genome shotgun sequence.
ACCESSION   DASTNL010000079 DASTNL010000000
VERSION     DASTNL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169416"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /gene="pgk"
                     /locus_tag="VFQ96_02660"
     CDS             <1..644
                     /gene="pgk"
                     /locus_tag="VFQ96_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU0256721.1"
                     /translation="PYTVVLGGSKVSDKLGVIEHLLPRVDSLLIGGGMLFTFLAAQGH
                     KVGASLLETDQLETVRGYLARASELGVQLLLPEDVVVAERFSADAAHVVSDVDAIEET
                     PWGAAGIGLDIGPRTAAAFAKVVAASQTVFWNGPMGVFEFPAFAAGTRALAEALSLAP
                     GLSVVGGGDSAAAVRTLGFSDDAFGHISTGGGASLEFLEGKELPGLEVLGWQQ"
     gene            632..1432
                     /gene="tpiA"
                     /locus_tag="VFQ96_02665"
     CDS             632..1432
                     /gene="tpiA"
                     /locus_tag="VFQ96_02665"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0256722.1"
                     /translation="MAAVSPTLRRPFIAGNWKMNLDHLQAIAVVQKLAWSLADAHHRH
                     DEVEVSVFPPFTDLRSVQTLIDADKLDITFGAQDVSEHESGAFTGEVSASLLARLGCR
                     YVIVGHSERRALHGESDEVVARKAAAAHAHGLVPVVCVGENAEDRERFGQAAVPIKQL
                     RAVVAALPRDAEFVVAYEPVWAIGSGNPATPEEAQAVAAALRELVADTLGDEAAGRTR
                     IVYGGSVKAANIAGFMRQPDVDGALVGGASLKPEEFSAIAQYRNHVGV"
     gene            1502..1747
                     /gene="secG"
                     /locus_tag="VFQ96_02670"
     CDS             1502..1747
                     /gene="secG"
                     /locus_tag="VFQ96_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0256723.1"
                     /translation="MAILSIVLQVLLGITSLLLTLMILLHKGNGGGLSDLFGGGVTSN
                     LGASGVAERNLNRITIVFGIIWIACVIVLGLITRFTA"
     gene            1758..2126
                     /locus_tag="VFQ96_02675"
     CDS             1758..2126
                     /locus_tag="VFQ96_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEU0256724.1"
                     /translation="MASGGSAIRGSRVGAGPVGEQDHGFHAERRAVSYWDALGHETVR
                     YFAADVADEDIPDTIASASTGLPAGRDKANPPSAEKNEPYKTHLAYVKERRSEEEAAA
                     LLEGALRQLRERRGKLTATN"
     gene            complement(2176..>2378)
                     /locus_tag="VFQ96_02680"
     CDS             complement(2176..>2378)
                     /locus_tag="VFQ96_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU0256725.1"
                     /translation="PVLNSSVRIWLVMAGSDKASALGLALAGAAYTEVPVAGAKGRER
                     TVFFVDKEAAAEVPENLISPSY"
BASE COUNT          375 a          814 c          816 g          373 t
ORIGIN      
        1 agccttacac cgtcgttctc ggaggctcca aggtctccga caagctgggg gtcatcgagc
       61 atctcctgcc gcgggtggat tcactgctca tcggcggcgg tatgctcttc accttcctgg
      121 ctgcccaggg ccacaaggtg ggcgccagcc tgctcgagac cgaccagctg gagaccgttc
      181 gcggctatct cgcccgtgcg agtgagctcg gcgtgcagct tctcctgccg gaagacgtcg
      241 tcgtcgccga gcgcttctcg gccgacgcgg cgcacgtcgt cagcgacgtc gacgcgatcg
      301 aagagacgcc gtggggggcc gccggcatcg gcctcgacat cggcccgcgc accgccgcgg
      361 ccttcgcgaa ggtcgtcgcg gcctcgcaga ccgtcttttg gaacggcccg atgggcgtct
      421 tcgagttccc cgcgttcgcc gcaggcactc gcgcgctcgc cgaagccttg agcctggcgc
      481 ccgggctctc tgtcgtcggc ggcggcgact cggcggccgc ggtgcgcacg ctgggcttct
      541 ccgacgacgc tttcgggcat atctcgacgg gcggcggcgc gagcctggaa ttcctcgaag
      601 gcaaagagct ccccggactg gaggtcctcg gatggcagca gtgagcccga cgctaaggcg
      661 cccgttcatc gccggcaatt ggaagatgaa cctcgaccac ctgcaggcga tcgccgtggt
      721 gcagaagctc gcgtggagcc tcgccgacgc ccaccaccgg cacgacgagg tggaggtctc
      781 ggtgttcccg ccgttcacag acctgcgcag cgtgcagacg ctcatcgacg cggacaagct
      841 cgacatcacg ttcggggcgc aagacgtctc ggagcacgag tccggagcgt tcaccgggga
      901 ggtcagcgcg agccttctcg cacggctcgg ctgccggtac gtgatcgtcg gccattcgga
      961 acggcgcgcg ttgcacggcg aatccgacga ggtcgtcgcc cgcaaggcgg cggccgccca
     1021 cgcgcacggc ctcgtgccgg tggtctgcgt cggcgagaac gccgaagacc gggaacggtt
     1081 cgggcaggcc gcggtgccga tcaagcagct gcgggccgtc gtcgccgcgt tgccgcggga
     1141 cgcggagttc gtcgtggcct acgagccggt ctgggcgatc gggtccggca acccggcgac
     1201 cccggaagag gcgcaggccg tcgcggcggc actgcgcgag ctcgtcgcgg acaccctggg
     1261 agacgaggct gcaggccgca ccaggatcgt ctacggcggc tccgtcaagg ccgccaacat
     1321 cgcggggttc atgcgccaac ccgacgtcga cggtgccttg gtcggcggcg cgagcctcaa
     1381 gccggaggag ttttcggcga tcgcgcagta ccgcaatcat gtcggcgtct agccgcgacg
     1441 tatactaagc atcgtccggc ggcttcgccg tggcggcgaa acccgaaagg taattccgag
     1501 cgtggccatc ctctccatcg tcttgcaggt tctgctcggc atcacgagcc tgctcctgac
     1561 gctgatgatc ttgctgcaca agggcaacgg cggggggctc tccgacctct tcggcggcgg
     1621 cgtgacctcc aacctggggg cgtcgggcgt ggccgaacgc aacctgaacc gcatcaccat
     1681 cgtcttcggc atcatctgga tcgcctgcgt gatcgttctc ggtctgatta cccgtttcac
     1741 ggcctaggag ggatgggatg gcgtcaggag gaagtgctat tcgcggctcc cgtgtggggg
     1801 ccggccctgt cggcgagcag gatcacggct ttcacgcaga gcgccgagcc gtgagctact
     1861 gggatgcgct gggccacgag acagtgcgtt atttcgcggc cgacgtcgcc gacgaagaca
     1921 tccccgacac catcgcgtcg gcctccaccg gcctgccggc cggccgagac aaggcgaacc
     1981 cgccgtcggc agagaagaac gagccgtaca agacacacct cgcctacgtc aaggaacgcc
     2041 gtagcgaaga agaggcggcc gcgctcctcg agggtgccct gcgccagctg cgggagcggc
     2101 gcggcaagct gaccgccacg aattgaccct catccgtcgt ctggccaccg tccggcgacc
     2161 agacgacgga tggcgtcagt agctcggcga gatgaggttc tccggaactt cggccgcggc
     2221 ctctttgtcg acgaagaaca cggtacgctc ccgtcccttg gctccggcga ccggaacctc
     2281 ggtgtatgcg gcgcccgcca gcgcgagccc gagcgccgaa gccttgtcgc tgccggccat
     2341 gacgagccag atgcgcaccg acgagttcaa gaccggcc
//
LOCUS       DASTNL010000080         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_243923, whole genome shotgun sequence.
ACCESSION   DASTNL010000080 DASTNL010000000
VERSION     DASTNL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243923"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VFQ96_02685"
     CDS             complement(<1..165)
                     /locus_tag="VFQ96_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU0256726.1"
                     /translation="MPKVADSLARLSALAASPAVEAVARAFEEAGFELALVGGPVRDA
                     LLGRPVHDLDF"
     gene            302..2545
                     /locus_tag="VFQ96_02690"
     CDS             302..2545
                     /locus_tag="VFQ96_02690"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEU0256727.1"
                     /translation="MSRRIPEILRWAASALVAGAMLAAFGAPAHAQSPETTPAPAASG
                     ISLTVAVPNGVAAEHGSTTVTALVTNQDGLFLPSGTVSFWRAGQLTDRAAIRRWDAGV
                     GAEPPGSTTALGSATVPSLAPGESASVTASFPRSALGASRHAPTVYSLGATLSMTGAA
                     TVRAHGTVLVHQSTPAAKQPHLAVAMPITVPASTSGVIDAADLARYTAPHGVLTREIS
                     GLEGHPEVAVGIDPKIIASIRVLGGAAPRSAIAWQQQLVNLPNEIFPLQYGDADAAAE
                     VQAGFPKLLTPTSFDWVTGPANVHSTTDAIGEPATQPPETTSPAPKPTGPVLPTSADI
                     LAWPYTLSGIVWPPEDVLSPADVTAFRKNNLTTVIASSTNVTGFDENAPQAHFRSGGA
                     DVLVSDNALSQDLREAVSAGTDTQFNAAMAAVNAQLAVLGGGQETVLATLSRASAGGS
                     AEWARVLESLDTSPGERLVPLSDVSAQPAAPGLTLAKKSEPRGRISDVESLADTAKKV
                     GSFASTLVEPRLLTGQERVRLLSLLGVAWLEPDADWHGAFERATARSSAILSAISIIP
                     SGLINVASTEAPLPVTVENKLAYPVNLLLVAAPSNARLEIDSTARKTIPSDARGTVLV
                     PVKARLGNGKVDLSLQLYSATGVPVGPSALIPVDVHAQWEGVAATVLAALIVGLFGFG
                     IARNVRRHVRARRATEADVEVPGDGEVSGADGGAGATSSVAGAAGEAPTDHETGTRPT
                     GGAATDG"
     gene            2538..>3492
                     /locus_tag="VFQ96_02695"
     CDS             2538..>3492
                     /locus_tag="VFQ96_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU0256728.1"
                     /translation="MAEIGRASVVLASGTVVSRVLGFVNMFVLASAIGSFGAAADTFA
                     AANQLPNTVHMIVVGSVVSAVFVPQVVRASLAEDGGQAYVNKLVTLTLVVLLAATGIA
                     LALAPILVRVTVSGFSGPKIVLTTIFAYWCLPQVFFYGLYTVLGEILNARKVFGPTTW
                     APVFNNVVAIIGVGIFIAAFGADPGGSRAVTHWTGGMVMTIGASASLGVACQALMLLA
                     FWRRAGLRYRPDFHWRGVGLRDTGRMAGWTFGMLAVTTLAGLAKSNVASRAATGDPSV
                     NVLAKSWLIFMLPHSVVTVSVATAYFTRMSEASAAGRLRAVK"
BASE COUNT          517 a         1283 c         1177 g          515 t
ORIGIN      
        1 gaagtcgagg tcgtgcacag ggcgaccgag gagggcgtca cgcaccggcc ccccgacgag
       61 cgcgagctcg aaccccgcct cctcgaaggc gcgtgccacc gcctcgacgg ccggcgaggc
      121 cgcgagcgcg ctcagccgcg caagggagtc ggcgaccttg ggcatgattt ctcattttat
      181 gggcaagccc gggcgccgac ccccgaacgc cacccgtggg gcgctcacca gggcaccata
      241 gaattgcgca catgccgacc ccggtggagc cacgcgcacg ttcgcgcgct gcgacgaccc
      301 gatgagccgg cggatcccag aaattctccg ctgggccgcc tcggcgctcg tcgccggcgc
      361 gatgctggcg gcgttcggcg cccccgccca cgcgcaaagc cccgagacga cacccgcgcc
      421 ggccgcctcc gggatctccc tgacggtcgc cgtgccgaac ggcgtcgccg ccgaacacgg
      481 ctcgacgacc gtgaccgcgc tcgtgacgaa ccaagacggc ctgttcctcc cctccggaac
      541 cgtctcgttc tggcgggccg gccagctgac ggatcgggcc gcgatccgca ggtgggacgc
      601 cggcgtcggt gcggaacccc cgggttcgac cacggcgctc ggcagcgcca ccgttccctc
      661 gctggcgccc ggcgagtcgg cgagcgtcac tgcctccttt ccccgttccg cgttgggggc
      721 aagccggcac gcgcctaccg tgtacagcct gggcgcgacg ctctccatga cgggcgccgc
      781 cacggtgcgc gcgcacggca cggtgctggt gcatcagagc acgcccgcag ccaagcagcc
      841 gcacctcgcc gtcgccatgc ccatcaccgt gccggccagc acgagcggcg tgatcgacgc
      901 cgccgacctg gcccggtaca ccgcgccgca cggcgtcctg acacgggaga tctccggcct
      961 ggaagggcac ccagaggtcg cggtcggcat agacccgaag atcatcgcgt cgatccgggt
     1021 gctcggcggc gcggcgccac gctcggcgat cgcctggcag caacagctgg tcaacctgcc
     1081 caacgagata ttcccgctgc aatacggcga cgccgacgcg gcggccgagg tgcaggccgg
     1141 ctttccgaag cttctgacgc cgacctcgtt cgactgggtc accgggccgg ccaacgtgca
     1201 cagcacgacg gatgcgatcg gtgagcccgc cacccaaccc cccgagacga cgagcccggc
     1261 accgaagccc accgggccgg tgctgccgac aagcgccgat attctcgcct ggccatacac
     1321 gctctccggc atcgtctggc cgccggaaga cgtcttgtcg cctgccgacg tgaccgcctt
     1381 tcgcaaaaac aacctcacca cggtgatcgc gtcgtcgacg aacgtgacgg gcttcgacga
     1441 gaacgcaccc caggcgcatt ttcgaagcgg cggcgctgac gtgctcgtct ccgacaacgc
     1501 tctgtcacaa gacctgcgag aagccgtgtc tgctggcacc gacacgcaat tcaacgcggc
     1561 catggcggcc gtgaacgcgc agctggcggt gctcggcggc ggccaggaga cggtgctcgc
     1621 gaccctgtcc cgcgcgagcg cgggcggcag cgcagagtgg gctcgggtgc tcgagtcact
     1681 cgacaccagc cccggcgagc gcctcgtacc gctctcggat gtctcggcac agccggctgc
     1741 gcccggcctc accctcgcga agaagtcgga gccccgcggc cgcatctccg acgtggagag
     1801 cttggccgac acggcgaaga aggttggctc cttcgcctcc accctcgtcg aaccccgcct
     1861 tctcaccggg caagagaggg tgcgccttct cagcctgctc ggggtggcct ggctcgagcc
     1921 ggacgccgac tggcacggtg cgttcgagcg agcgacggcg cgcagctcgg ccattctgag
     1981 cgcgatatcg atcatcccga gcggcctcat caatgtggcc agcaccgagg ccccgctgcc
     2041 ggtgaccgtc gaaaacaagc tcgcctaccc ggtgaatctg ctcctggtcg ccgccccgtc
     2101 gaacgcccgc ctcgagatcg acagcacggc ccgcaagacg atcccgtccg acgcgcgcgg
     2161 aacggtgctc gtgccggtga aggccaggct cggcaacggc aaagtggatc tcagcctgca
     2221 gctttatagc gccacgggtg ttccggtcgg cccatccgcg ctcatcccgg tcgatgtgca
     2281 tgcgcagtgg gaaggtgtcg cggccaccgt gctcgccgcc ctgatcgtcg ggctgttcgg
     2341 gttcggcatc gcccgcaacg ttcgtcggca cgttcgcgcg cgccgcgcga ccgaggccga
     2401 cgtcgaggtc cccggggacg gcgaggtctc cggcgctgac ggaggcgccg gggcgaccag
     2461 ctccgtcgct ggggccgccg gcgaagcccc gaccgaccac gagaccggca cgaggccgac
     2521 cggcggggcg gcgacggatg gctgagatcg gcagggcgag cgtcgtcttg gcctcgggca
     2581 ccgtcgtgtc gcgggttctc gggttcgtga acatgttcgt cctcgcctcg gcgatcggct
     2641 ccttcggcgc ggcggccgac acattcgccg cagcgaatca gctgcccaac acggtgcata
     2701 tgatcgtggt cggcagcgtg gtcagcgccg tgttcgtacc gcaggtggtg cgggcgagcc
     2761 ttgccgaaga cggcggccag gcctacgtca acaagctcgt caccctcacc ctcgtcgtgc
     2821 tcctcgcggc gacaggcatc gccctggcct tggccccgat cctcgtgcgg gtgaccgtgt
     2881 ccggcttctc cgggccgaag atcgtgctca ccacgatctt cgcgtattgg tgtctgccgc
     2941 aggtcttctt ctacggcctc tacacggtgc tcggcgagat tctgaacgcc cgcaaggtgt
     3001 tcggccccac cacctgggca ccggtcttca acaacgtggt cgcgatcatc ggcgtcggca
     3061 tcttcatcgc cgcattcggc gcagaccccg gcggaagccg cgccgtcaca cactggacgg
     3121 gaggcatggt gatgacgatc ggggcgagcg cgagcctcgg ggtcgcctgc caggccctca
     3181 tgcttctcgc cttctggcgc cgtgccggcc tgcgctaccg gcccgacttc cattggcgtg
     3241 gcgtcgggtt gcgcgacacg gggcgcatgg cggggtggac cttcgggatg ctggccgtga
     3301 cgacgctcgc ggggctggcc aagagcaacg tcgcctcccg cgcggcgacc ggagacccct
     3361 ccgtgaacgt gttggcgaag tcgtggctga tcttcatgtt gccgcactcg gtcgtcaccg
     3421 tctctgtggc caccgcctac ttcacccgga tgagcgaggc gagcgcggcc gggcgcctcc
     3481 gggcggtgaa ag
//
LOCUS       DASTNL010000081         5029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1172568, whole genome shotgun sequence.
ACCESSION   DASTNL010000081 DASTNL010000000
VERSION     DASTNL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172568"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..948)
                     /locus_tag="VFQ96_02700"
     CDS             complement(145..948)
                     /locus_tag="VFQ96_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU0256729.1"
                     /translation="MTRIGHDSDHDVVVTPAGQRIGLSWSGLTDIGKRRAVNEDSLVT
                     KPPIFAVADGMGGHSAGDVASAAVVTRLAGEAAARFTARGALDRALRRAVVDIERRAG
                     HSDLGTGTTVTGVALTLVGDEPYWLVFNIGDSRVYRQTTSRGLERLTIDHSVVQELVD
                     AGEITADEADGHPQSNIITRAVGFNELPVPDYRLVPLDPGSRLLLCSDGLTKEVGEKK
                     IAAVLAHGRPASATAGDLVELALGGGGRDNVTVVVVDVAPFPGAAHGEG"
     gene            complement(1071..1706)
                     /locus_tag="VFQ96_02705"
     CDS             complement(1071..1706)
                     /locus_tag="VFQ96_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 family protein"
                     /protein_id="HEU0256730.1"
                     /translation="MEHLDRGSPAAGPDDCAALAARIALWENALRSGESSRADVVASL
                     TDSIGSPCGRDVALLQVAFGRQAALLAAESDARWQAARRAGLAPFAPGGARASIAWAG
                     GSPADAARVDAFVGDLVAGHSDIRPDPARMRRGIDLLVAVTRAADAGRRAQAHSMIAW
                     LQWAGGRPAEADRHVGYALEAEPGHVFARLLRGMFRSGWRPHWLIDTPVTA"
     gene            complement(1827..3869)
                     /locus_tag="VFQ96_02710"
     CDS             complement(1827..3869)
                     /locus_tag="VFQ96_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256731.1"
                     /translation="MSTPAGRRPAAGGHRGPFRGGGAPAQKARNFGPSAKRLLGTLRP
                     DAVWLGIVVALGVVSVALSVWGPKLLGDATNVVVAGFVSRQFRNLPAGVTRGDVIAQL
                     QHHGQANVADMLRSVQFTPGQGMDFGKLGLIVGTVTAVYILSAVFMWLQGRILNIVTQ
                     RAMHRLRMQVEDKLHTLPLRYFDRMPRGELISRVTNDVDNIGQTLQQSMSQVVISVLT
                     VVGVLVMMFTISPLLAVIALVTVPLTLLVTVLVAKRSQKLFIAQWDATGKLTTRVEET
                     FSGHAIVKVFGHQQDARDRFRAENDAVYRASFGAQFVSGIIMPAMMFIGNLVYVAIAV
                     VGGIQVAGGLISIGAVQAFIQYSRQFTQPLSQLGSMANLLQSGVASAERVFELLDEPD
                     EEPDARDSETVPDEAGHLAFEEVGFRYVADQPLIRDVSLAATPGSSVAIVGPTGAGKT
                     TLVNLIMRFYDVDSGRITLDGVSTRQMTRADLRRRTGMVLQDTWLFSGTIRENIAYGR
                     PGASEEEITRAATAAYVDRFVHALPDGYDTVLNDEATNLSAGERQLITIARAFLADPH
                     ILILDEATSSVDTRTELLIQRAMSALAADRTSFVIAHRLSTIRDADLILVMEHGDIVE
                     QGTHERLLAARGAYWRLYNAQFEAPTLDAGAPAIPAGGAPAAAVLPDALGASGSVE"
     gene            complement(3866..>5029)
                     /locus_tag="VFQ96_02715"
     CDS             complement(3866..>5029)
                     /locus_tag="VFQ96_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256732.1"
                     /translation="DRVNQVMREQLAGIRVVRAFVREGQERSRFEDASVNVRDYATTA
                     GNLMAIMFPVVMLVLNVSGAAVIWFGAAQVDHGDAQIGTLFAFLTYLMQILMGVMMAT
                     FMFVMIPRAAVCAGRIGEVLDTRPSVAAGASHAEVPAPEGLVEFEHVDFTYPGADDAV
                     LKDLTFTVRPGTTTAIIGATGSGKSTLIGLVPRLFDVTAGSVRVDGVDVRDYDPDELW
                     TRIGLVPQRAFLFSGTVASNLRYGDESATDDDLWQALAIAQADGFVRRMPEGLESPVA
                     QGGTDVSGGQRQRLAIARALVRSPEIYIFDDSFSALDLRTDARLRAALDEHLPNATRI
                     IVAQRVSTIQHADQIIVIDHGRVVGVGRHDELRETNDTYREIVDSQLAAEETV"
BASE COUNT          779 a         1724 c         1793 g          733 t
ORIGIN      
        1 ggcaggccgt ccctcaccgg acccgtctcg tcgggatctc gcctgcgcat gggaccagtg
       61 aaccgggccg cagggccgga tcggcaggcc gaggagaaac ctgaggaaaa tctccgccgc
      121 cgtggcgggg tggcggacgg gtgctcagcc ttcgccgtgc gcggccccgg ggaacggggc
      181 gacgtcgacg acgacgacgg tcacgttgtc gcggcccccg ccgcccagcg cgagctcgac
      241 gaggtcgccg gccgtcgcgg aggcaggccg gccgtgggcg agaaccgccg cgatcttctt
      301 ttcgccgact tctttcgtca gcccgtcgga gcacagaagc agccggctgc cggggtcgag
      361 cggcacgagc cgatagtcgg gcaccggcag ctcgttgaag ccgacggcgc gggtgatgat
      421 gttgctctgc ggatgcccgt ctgcctcgtc ggcggtgatc tctcccgcgt cgacgagctc
      481 ctgcacgacc gaatggtcga tggtcagccg ttcgagcccc cggctcgtcg tctgtcggta
      541 gacacgggaa tcgccgatgt tgaagaccag ccagtacggt tcgtcaccga ccaacgtcaa
      601 cgcgacgccg gtcaccgtcg tgccggtgcc caagtcgctg tgccccgcgc ggcgctcgat
      661 gtcgacgacg gcacgacgca gcgcccgatc gagcgcgccg cgcgcggtga agcgtgccgc
      721 cgcctcgccg gccaaccgcg tgacgacggc ggcactggcg acatcgcccg cgctgtgccc
      781 acccatgccg tcggcgaccg cgaagatcgg cggcttcgtc accaggctgt cctcgttcac
      841 cgcgcggcgt ttgccgatgt cggtcaaacc agaccaggag aggccgatac gctggccggc
      901 cggtgtcacg acgacgtcgt ggtcgctgtc gtgcccgatc cgagtcacgc gcatcccttc
      961 cgcatcacct cgggcccctg tgagttcccg tcgggtgcct cacgtcgggc gccacgcccg
     1021 cgcaagccgg ctcgtgccag agccccggca cgctcgtcga tcgtacagcc tcacgcagtc
     1081 accggcgtgt ctatgagcca gtgcgggcgc cacccagagc ggaacatgcc ccgcagcagc
     1141 cgggcgaaga cgtggccggg ttccgcctcg agtgcgtagc cgacgtgccg atccgcctca
     1201 gccgggcgtc cgcccgccca ctgcagccag gcgatcatgc tgtgagcctg cgcccgccgc
     1261 ccggcgtcgg cggcccgggt gacggcgacg agcaggtcga tgccgcggcg catccgcgcc
     1321 gggtcggggc ggatatcgct gtgccccgca acgaggtcgc cgacgaaggc gtcgacgcga
     1381 gcggcgtcgg ccggcgaccc gccggcccat gcgatgctcg cgcgggcgcc gcccggtgca
     1441 aacggtgcga ggccggcgcg acgggccgcc tgccagcggg cgtcgctttc ggccgcgagc
     1501 aacgcggctt gccgcccgaa ggcgacctgg agaagcgcga cgtcacggcc gcagggcgag
     1561 ccgatgctgt cggtgagaga ggctacgacg tcggcccggg acgactcgcc agagcgtaac
     1621 gcgttctccc acagggcgat acgggccgcg agcgccgcgc agtcgtcagg tccggccgcc
     1681 gggctgcctc gatcgagatg ttccatcgcc acagcctcgc cggccggcca caggcaccgg
     1741 gatgcgaacc aggaaccatg gagaaccggt gccgcgggcg cagcctgtgg aggggcaagg
     1801 agagcccacg aggcaccccg cccccgctac tcgaccgagc ctgacgcgcc gagcgcatcc
     1861 ggcaacacgg cggccgccgg ggctcccccg gccggtatcg ccggcgcccc ggcgtcgagg
     1921 gtgggtgcct cgaactgcgc gttgtacagc cgccagtagg ctccccgtgc ggccagcaac
     1981 cgctcgtggg tgccctgctc gacgatgtcg ccgtgctcca tcacaaggat gaggtcggcg
     2041 tcacggatgg tcgagagccg gtgggcgatg acgaacgacg tgcggtcggc ggcgagcgcc
     2101 gacatggccc gctggatgag cagctccgtg cgggtgtcga cactgctcgt cgcctcgtcg
     2161 aggatcagga tgtgcgggtc ggcgaggaag gcgcgggcga tggtgatcag ctgccgctca
     2221 cccgccgaaa gattcgtcgc ctcgtcattc agaaccgtgt catatccgtc gggcaacgcg
     2281 tgcacgaacc ggtcgacgta ggcggcggtg gccgccctgg tgatctcctc ctcgctcgcg
     2341 cccggccgcc cgtaggcgat gttctcgcgt atggttccag agaacagcca ggtgtcttgc
     2401 agcaccatgc cggtacggcg ccgaaggtcg gcgcgcgtca tctgccgggt gctcacgccg
     2461 tcgagggtta tgcggccgga gtcgacgtcg tagaagcgca tgatcaggtt gacgagggtc
     2521 gtcttgccgg ccccggtcgg cccgacgatc gcgacgctcg aacccggcgt cgcagcgagg
     2581 ctcacgtcgc ggatcagagg ctggtcggcc acgtaccgaa acccgacctc ctcgaaggcg
     2641 aggtgcccgg cctcgtcggg cacggtctcg gagtcccggg catccggctc ctcgtcgggc
     2701 tcgtcgagga gttcgaacac gcgctcggca ctcgcgacgc cggactgcaa caggttcgcc
     2761 atggagccca gttggctgag gggctgcgtg aactggcggg agtattgaat gaaggcctgc
     2821 accgcgccga tggagatgag gccgcccgcg acctggattc cgccgaccac cgcgatggcg
     2881 acgtagacga ggttcccgat gaacatcatg gcgggcatga tgatgccgga gacgaactgg
     2941 gcgccgaagc tcgcacggta caccgcgtcg ttctccgccc ggaaccggtc gcgggcgtct
     3001 tgctggtggc cgaagacctt gacgatggcg tggccggaga acgtctcttc cacccgggtg
     3061 gtgagcttgc cggtcgcatc ccactgcgcg atgaaaagct tctgcgagcg cttggccacc
     3121 aacaccgtga cgaggagggt caagggcacg gtgacgaggg cgatcacggc gaggaggggc
     3181 gagatcgtga acatcatgac gagcacgccg acgaccgtga gcaccgagat gacgacctgc
     3241 gacattgact gctgcagcgt ctggccgatg ttgtcgacgt cgttggtgac ccggctgatg
     3301 agctcgccgc gcggcatacg gtcgaagtaa cgcagcggca gggtgtgcag cttgtcttcc
     3361 acctgcatgc gcaggcggtg catcgcgcgc tgcgtgacga tgttcaggat gcggccctgc
     3421 agccacatga acacggccga caggatgtag acggctgtga cggtgccgac gatgaggccc
     3481 agcttgccga agtccatgcc ctggccgggc gtgaactgca cgctgcggag catgtcggcg
     3541 acgttcgcct ggccgtgatg ctgcagctgc gcgatgacat cgccgcgggt gacgccggcc
     3601 ggcaggttgc ggaactggcg ggagacgaag cccgcgacca ccacgttggt ggcatctccc
     3661 aggagcttcg gcccccagac gctcaaggcc acgctgacga cgcccaaggc gacgacgatc
     3721 ccgagccaga cggcgtcggg ccgcagcgtt ccgagcagcc gcttggcgct ggggccgaag
     3781 ttgcgggcct tctgcgccgg cgccccgccg cctcggaacg ggccgcggtg cccgccggct
     3841 gccggcctac gcccggccgg ggtgctcata ccgtctcctc cgccgcaagc tgcgaatcga
     3901 cgatctcgcg atacgtgtcg ttcgtctctc gcagctcgtc gtgcctgccc acgccgacga
     3961 cgcggccgtg gtcgatgacg atgatctggt ccgcgtgctg gatggtggag acccgctgcg
     4021 cgacgatgat gcgggtcgcg ttcggcagat gctcgtcgag agccgcccgc agtcgcgcgt
     4081 cggttcgcag gtcgagggcg gagaacgagt cgtcgaagat gtagatctcg gggctgcgca
     4141 cgagcgcccg ggcgatggcg aggcgctgcc gctgcccccc ggagacgtcg gtgccgcctt
     4201 gcgcgacggg gctttcgaga ccctccggca tccggcgcac gaacccgtct gcctgcgcga
     4261 tggcgagcgc ctgccacagg tcgtcgtcgg tggccgactc gtcgccgtag cgcaggttgc
     4321 tggccacggt gccggagaac aggaacgccc gctgcggaac gagcccgatg cgggtccaca
     4381 gctcgtcggg gtcatagtcg cgcacatcca ccccgtcgac acgcacgctg cctgcggtga
     4441 cgtcgaaaag ccgcggcacg aggccgatga gggtggattt gccggaaccg gtcgcgccga
     4501 tgatcgcggt cgtggtgccc ggccgcacgg tgaaggtgag atctttcaac accgcatcgt
     4561 cggctcccgg gtaggtgaag tcgacatgct cgaattcgac cagcccctcc ggagccggaa
     4621 cctctgcgtg ggaggccccg gcggcgaccg acggccgggt gtcgagcacc tcaccgatgc
     4681 ggcccgcaca cacggccgcg cgggggatca tgacgaacat gaaggtcgcc atcatcactc
     4741 ccatgaggat ctgcatgagg taggtgagaa aggcgaacag ggtgccgatc tgggcgtcgc
     4801 cgtggtccac ctgcgcggcg ccgaaccaga tcaccgccgc gccggagacg ttcagcacca
     4861 gcatgacgac ggggaacatg atcgccatca ggttgcccgc ggtcgtcgca tagtcgcgca
     4921 cgttcacgct ggcatcctcg aaccggctgc gctcctggcc ctcccgcacg aacgcgcgta
     4981 cgacgcggat gccggcgagc tgttcgcgca tcacctggtt gacccggtc
//
LOCUS       DASTNL010000082         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_617368, whole genome shotgun sequence.
ACCESSION   DASTNL010000082 DASTNL010000000
VERSION     DASTNL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617368"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1994
                     /locus_tag="VFQ96_02720"
     CDS             168..1994
                     /locus_tag="VFQ96_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0256733.1"
                     /translation="MAHLLGAEGLGLAYPTRAVFDAVTLGIGDGDRIGIVGRNGDGKS
                     SLLRMLSGDLTPDAGRVTRRSGVTVGLLSQADALDDDETVGHAVVGDRPEHEWAGDAR
                     TRDVLAGLVPDLDIRARIGTLSGGQRRRVALAALLVGEWDIVALDEPTNHLDVEGIAW
                     LARHLKTRWARNAGGLLVVTHDRWFLDEVATTTWEVHDRVVEPFDGGYAAYVLQRVER
                     DRQAAAKEARRQNLMRKELAWLRRGAPARTAKPRFHIEAANRLIADVPPPRDRLELSR
                     MAVARLGKDVVDLVGAAASFDGREVLRDVEWRLSPGERSAVLGANGAGKSTLLGLIAG
                     TVQPSAGRVKRGATVRLGVLDQRFRELEEIADDRVREVLARAKTSYVVGGKELTPAQL
                     LERLGFSHEHLSARVGELSGGQKRRLQLLLVLLDEPNLLLLDEPSNDVDTDFLAAMED
                     LLDSWPGTLIVVSHDRYLVERVTDQQYAILPGDDGVGRLRHLPGGVEEYLRLKTAAGH
                     ADADARAVSSAEAGHKSAGPEHPGVRLSGAERRAIEKELAALERRIDRLTRQAGDARA
                     GLASLDQSDYQLLDSRMAKITAMEAEAEGLEERWLELSTAAE"
     gene            complement(2025..2630)
                     /locus_tag="VFQ96_02725"
     CDS             complement(2025..2630)
                     /locus_tag="VFQ96_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0256734.1"
                     /translation="MSLSEDSGSPAPKRRRGEALEAALLDAAWEELSDKGYDRFTIDS
                     VAARARTSRAVVYRRWQGKAELIRAALVRAGTRESAPVPDTGSLRGDVIELLRQANRS
                     RAQIGVQMILQLGGYYAETGEGIAELRGAFLSRRSSSMQKVLERAVERGEVDPAKLTP
                     RVVSVPFDLYRHELMMTLKAVPDEVAQAIVDEVFLPLVVKR"
     gene            2690..>3063
                     /locus_tag="VFQ96_02730"
     CDS             2690..>3063
                     /locus_tag="VFQ96_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256735.1"
                     /translation="MPATKTESTKVDAAVWKTIGAVLVGALAVLFDTTIVAVALHTLA
                     ADLHTSVATIQWVTTGYLLALGVTVPITGWAQKVVGGKRLWIFALALFLAGSVLSSLA
                     WSAGSLIAFRVVQGAGGGVMLPL"
BASE COUNT          474 a         1083 c         1049 g          457 t
ORIGIN      
        1 cacgttcctt ctcccggccg cacgccttag cggcccgata cgtttttctc aaagagtcct
       61 agcctaatgc acagcggccg caccgcaccg cgtaccaccg accacgcagg cctgccgccg
      121 gcggccggga cgcacaccgc ctcccgctcc tctacggtgg aagggtcatg gcacatcttc
      181 tcggcgcgga aggcctcggc ctcgcctacc cgacccgcgc ggtcttcgac gcggtcacgc
      241 tcgggatcgg cgacggcgac cgcatcggca tcgtcggccg caacggtgac ggcaagtcgt
      301 ccctcttgcg catgctgagc ggcgacctga cgccggatgc cggccgagtg acccgccgca
      361 gcggcgtcac ggtgggactc cttagccaag ccgacgcgtt ggacgacgac gagacggtcg
      421 gccacgcggt ggtcggcgac cggcccgagc acgaatgggc gggcgatgcc cggacccgcg
      481 acgtgctcgc cgggctcgtg ccggatctcg acatccgggc gcggatcggc acactttcgg
      541 gcgggcagcg ccgcagggtc gccctcgccg cgctgctcgt gggcgagtgg gacatcgtcg
      601 cgttggacga gccaacaaac cacctcgacg tggagggcat cgcctggctt gcgcgccacc
      661 tgaagacccg ttgggcgaga aacgccggcg gcctcctcgt cgtcacccac gaccgttggt
      721 tcctcgacga ggtcgcgacg acgacgtggg aggtacacga ccgggtcgtc gagccgttcg
      781 acggcggcta cgccgcgtac gtgctgcagc gggtggagcg cgaccgacag gcggcggcga
      841 aggaggcgag acggcagaac ctgatgcgca aagagcttgc ctggctgcgc cgcggcgccc
      901 cggcccgcac cgcgaaaccc cggttccaca tcgaggcggc gaaccggctg atcgccgacg
      961 tgccaccgcc gcgcgaccgg ctggaactct cacgcatggc ggtggcgagg ctcggcaaag
     1021 acgtcgtcga cctcgtggga gccgcagcct ccttcgacgg ccgggaggtc ttgcgcgacg
     1081 tcgaatggcg cctttccccc ggcgagcgaa gtgccgtgct cggcgccaac ggcgccggca
     1141 agtcgaccct cttgggcctc atcgccggta ccgtgcagcc ctccgccggc cgggtgaaac
     1201 gcggcgcgac ggttcgcctc ggcgtcctcg accagcgctt ccgcgagctg gaggagatcg
     1261 cagacgaccg ggtgcgcgag gtgttggcgc gcgcgaagac gagctacgtc gtcggcggca
     1321 aggagctgac gcccgcgcag cttctcgaac gcctcggttt ctcccacgaa cacctctcgg
     1381 cccgggtcgg agagctctcc ggcggccaaa aacgcagact gcagctcttg ctcgtgcttc
     1441 tcgacgagcc gaacctgctg cttctcgacg agccgtcgaa cgacgtggac accgacttct
     1501 tggcggcgat ggaagacctg ttggattcct ggccgggcac cctcatcgtc gtctcgcacg
     1561 accgttacct cgtcgagcgg gtcaccgacc agcagtacgc gatcctgccg ggcgacgacg
     1621 gggtcgggcg cctacgccac ctgcccggcg gcgtggagga gtacctgcgg ctgaaaacgg
     1681 ctgcggggca tgccgacgcg gacgcccggg cggtgtcgtc ggccgaggcc ggccacaagt
     1741 cggcggggcc cgaacacccc ggcgttcgtc tctcgggcgc agaacggcga gcgatcgaga
     1801 aggagctggc cgcgctggag cgccgcatcg accgtctgac gcgccaggcc ggcgacgccc
     1861 gtgccgggct cgccagcctc gaccagtcgg attaccagct tctcgactcc cggatggcga
     1921 agatcaccgc gatggaggcc gaggccgagg gtctcgagga gcgttggctg gagctgtcga
     1981 ccgccgcgga gtgagcgatc ggccccgacg gcgacagtgc cgggtcagcg cttcacaacg
     2041 agcggcaaaa acacctcgtc gacgatggcc tgcgcaacct cgtcgggcac ggccttcaac
     2101 gtcatcatca actcgtggcg atagaggtcg aagggcaccg agaccacacg gggggtgagc
     2161 ttggccgggt cgacctcgcc gcgttcgacg gccctctcca gcactttctg catcgaactg
     2221 gaacggcggg agaggaaggc gccgcgcagt tcggcgatgc cctcgccggt ctcggcgtag
     2281 tagccgccca gctgcaggat catctgcacg ccgatctgcg cccgcgagcg gttcgcctgg
     2341 cgcagaagct cgatgacgtc gccgcgcagg ctacccgtgt cgggcaccgg agccgactca
     2401 cgcgtgccgg ctcgcacgag ggcggcgcgg atcagttcgg ctttgccctg ccaacgccgg
     2461 tagacgacgg cacggctcgt gcgggcgcgc gcggcgacgg agtcgatcgt gaacctgtcg
     2521 tagcccttgt cgctgagctc ctcccacgca gcgtcgagca gggcagcctc cagggcctca
     2581 ccccgccgac gcttcggtgc agggctccct gaatcttcac taagagacat tgtgtttctt
     2641 ctccccacgc cgtaacttag agatacagaa tgtaccttaa ggagcttcga tgcccgccac
     2701 gaagaccgaa tctaccaagg tcgacgccgc cgtctggaag acgatcgggg ccgtgctcgt
     2761 cggcgccctg gccgtgctct tcgacacgac catcgtcgcg gtcgccctgc acaccctcgc
     2821 cgccgacctg cacacctccg tcgccacgat ccaatgggtc acgacgggct atcttctcgc
     2881 cctcggcgtg accgttccga tcacgggctg ggcgcagaag gtcgtgggcg gcaagcggct
     2941 gtggatcttc gccctcgcgc tgttcctcgc agggtcggtg ctctccagcc tggcgtggag
     3001 cgcaggcagc ctcatcgcgt tccgcgtcgt gcagggcgcc gggggcggcg tgatgctgcc
     3061 gct
//
LOCUS       DASTNL010000083         8830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_247545, whole genome shotgun sequence.
ACCESSION   DASTNL010000083 DASTNL010000000
VERSION     DASTNL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247545"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFQ96_02735"
     CDS             complement(<1..808)
                     /locus_tag="VFQ96_02735"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192631.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEU0256736.1"
                     /translation="MDPRAGTPAEPSDLVDIEALIDAYHERRPDPSIPAERVVFGTSG
                     HRGSSLTTSFNEDHIAATTQAIVDYRNKEGIAGPLFLGADTHALSAPAVQTALRVLAA
                     NAVHVLVDSRDDVVPTPALSHAIVRHNRTLSQGDPGRADGIIVTPSHNPPSDGGFKYN
                     PPHGGPAGSDTTAWIANRANEIIADGMRAVGDGRARAGRGQGGADETDGAASRGEVGR
                     YDFREHYVADLARIINVSVIAASGVRIGADPLGGASVHYWEAIRDRYGLNL"
     gene            complement(889..1449)
                     /locus_tag="VFQ96_02740"
     CDS             complement(889..1449)
                     /locus_tag="VFQ96_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0256737.1"
                     /translation="MESIRDRLRAVPSFPDQMPALETEAVPDDPDRLFLGWLDDAIAS
                     GARQPNALSLATLSAEGTPVTRMVILKDRDERGYWFSTHRDSKKGLQVAANPRASMLF
                     FWRESGRQVRITGTVVPLDDETSQADWRTRPAYAGAPNPAWQVYALQATEFEFLQARL
                     DRKHVRVAYHKTTSGWTHARVTTPAG"
     gene            1577..2548
                     /gene="pheA"
                     /locus_tag="VFQ96_02745"
     CDS             1577..2548
                     /gene="pheA"
                     /locus_tag="VFQ96_02745"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU0256738.1"
                     /translation="MQTQPAGETYSFLGPSGTFTEAALAQVPEARGKAWRPVNNVPEA
                     LDDVVTGQSVAAMIAIENSIEGGVSVAQDALATTPGLRIVGEYLVPVSFILVARPGTR
                     LADVRTVNAHPVAYAQCHRWLNRTLPRHVHVPATSNVTAASALLESDAPGGPSDAAIA
                     PPTIVEHHDLDVLATGIGDDSNAMTRFVLVGRPPRLPAPTGADKTSLIAELPEERAGT
                     LLTMLQQFSTRGVNMSLIESRPVGDALGRYRFVIDLDGHIRDERVADALLGLHRFSPN
                     VIFLGSYPRADRQPVQFDPRYRDEVFTAARTWLGGIIDGGAEARRPF"
     gene            complement(2555..3577)
                     /locus_tag="VFQ96_02750"
     CDS             complement(2555..3577)
                     /locus_tag="VFQ96_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU0256739.1"
                     /translation="MGDADPAEVRFAAVVANPVKVNIERLRAAVERSSREHGWAAPVW
                     RETTVSDPGARAAREALSTGAALIITAGGDGTVRAVAGIVRGTGVPLAIVPAGTGNLL
                     ARGLNLPLNSIEKAVALAFTGEPRSVDVGVVRALPERASQSAHEHVFVVMAGMGVDAT
                     LLANTRSSLKKRVGWLAYVDGGMRSLVTLKPMHLRFAVDGGTPHGARISTILVANSGR
                     LPGGIDLFPDAAVDDGLLDVAVLQPRSIFGWLLVWRRVTWENRVLRRSALGRSLIRFT
                     GALLHVTRPGRRRTVTYLAGKEVTVTSREPQPFEVDGDPAGIARELHFRVEPGALVIK
                     TPRRLR"
     gene            3621..4889
                     /gene="serS"
                     /locus_tag="VFQ96_02755"
     CDS             3621..4889
                     /gene="serS"
                     /locus_tag="VFQ96_02755"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU0256740.1"
                     /translation="MIDPVVLRENPDLIRDSQRARGASVDLVDLAIEADAARRGALAA
                     FESLRAEQKQLGKKVAKAAKEEKAGLLAAAQDLAAQVKAASGEADVAVGRFESAVRRL
                     GNVVLAGVPAGGEENFVTLRTGGVKPRFDFTPRDHLELGEMLGAIDMARGAKVSGARF
                     YFLRGIGARLDLALMRLALDTALDAGFTPMITPTLVKPEIMQGTGFLGEHADEVYHLD
                     IDDLYLTGTSEVALAGYHADEILDFAHGPIRYAGWSTCYRREAGSGGKDTRGIIRVHQ
                     FNKLEMFVYTSPDQAAAEHERLVGWQEGLLASLGLHYRVIDVAAGDLGQSAARKYDIE
                     AWVPTQDTYRELTSTSNCTTFQARRLGTRYRTSLGETTPVATLNGTLATTRWIVALLE
                     THQQADGSVRVPEVLRPYLGVDVMTPVEAR"
     gene            4891..5697
                     /locus_tag="VFQ96_02760"
     CDS             4891..5697
                     /locus_tag="VFQ96_02760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0256741.1"
                     /translation="MGPAPRRRLVALDVDGTILNADDTVSRPVVEAIAAAARAGHEVM
                     LATGRSWEAAEPVMRKAGIRSRYVVCANGAITMKRDPRSPGGYRRFHIDTFDAHEVLA
                     TIRPRLPDGRFLVEDATGFRRYTEAAGDWLLERAEKVPFDRLAEVPATRVVVVSPGHD
                     EEDFFRVVESMGLHRVSYSIGWTAWLDISPEGVTKATALERVRELLGFERRDVICVGD
                     GRNDIEMLRWAAEEGRGVAMGHASDDVVAAANEVTGSIDDDGVAALLAAI"
     gene            5834..7195
                     /locus_tag="VFQ96_02765"
     CDS             5834..7195
                     /locus_tag="VFQ96_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU0256742.1"
                     /translation="MSDEPGGERRRGRRSALHGTLARHGRLPRSGPWRSILRFVAAGV
                     AVVVVSAFSIAAIATWDVAHTLEKNTVHLVDAKGNPVTPEVGTIAGAFNVLLVGTDTR
                     QGQGPGYGGLRESSGSGLNDVTMLLHVSADHDHATVVSLPRDMFVPIPSCPRPGGGFY
                     SAMSRQKINTTLRYGGLSCTVLTVQQLTGMTIPYAAEVTFDGVIAMSDAVGGVTVCTT
                     GTINDPHTGLHLAAGEHTLKGKQALEFLRTRHGVGDGSDLGRISNQQVFLSAMLRQMM
                     RAGTLTNLPEVYGLAKAAASNMRLTDSLNVSTLASMAMALKGVNLSKVAFIQYPNATL
                     PDGSGVIPLPQAAATLVTALQQGKSLTITGGTGVAGVGSVAQTPSPSGTGSASGSSLT
                     GSATGSPTTTSPGSPGTSSSTSAATDGTTGTGSPADEAVALPDTVHGQTAAEKTCSRA
                     YDF"
     gene            7375..8718
                     /locus_tag="VFQ96_02770"
     CDS             7375..8718
                     /locus_tag="VFQ96_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU0256743.1"
                     /translation="MSMVGGERRRGRRSTATATLARHGRLKRRGPWRSVLRFVVAGVA
                     VVAISAVSITAITVWDLSSTLTSHTIHLVDAKGKPVVPAVDSLKGAFNVLLVGTDTRT
                     GQGSGYGALTESEGAGQNDVTILLHVSANHNRATVVSYPRDMFVSVPSCPRPQGGFSP
                     AQVGRKINTTLIDGGLSCTVLTVEQLTGVTIPYAAEVTFDGVIAMSDAVGGVPVCVSG
                     TINDSHTHLHLTSGEHTLVGKEALQFLRTRHGVGDGSDLGRISNQQVFLSSLMRTIMA
                     RGTLTNISKVYGLAKAAATNMRLSDSLNISTLASMAVAMRHIDLSKVTFVQYPTTIVP
                     GGVDALQPAGNLLSQAIQHDQPITLTSGTGAPRVGSVVKGSSSSSSSGAKASTSGGSS
                     TGSASSGSSAPPASSAKGASPAPTPQASAPPSVVLPHGVYGQTATEQTCSRAFDF"
BASE COUNT         1317 a         3109 c         3081 g         1323 t
ORIGIN      
        1 cgaggttcag cccgtatcgg tcgcggatcg cctcccagta gtgcacgctc gccccgccca
       61 acgggtcggc gccgatccgg acgcccgagg cggcgatcac cgacacgttg atgatccggg
      121 cgagatccgc gacgtagtgc tcgcgaaagt cgtaccggcc gacctccccg cgtgaggctg
      181 cgccgtccgt ctcgtctgcg cccccttgcc cccggccggc ccgcgcccgg ccgtcgccga
      241 cggcgcgcat gccgtcggcg atgatctcgt tcgcacggtt cgcgatccag gcagtcgtgt
      301 cgcttcctgc cggcccgccg tgcggcgggt tgtacttgaa accgccgtcg gagggcgggt
      361 tgtggctcgg agtgacgatg atcccgtcgg cgcgtcccgg atcgccttgt gacagcgtgc
      421 ggttgtggcg cacgatcgcg tgggagaggg ccggtgttgg cacgacgtcg tcgcgagaat
      481 cgacgagcac gtgcaccgcg ttcgcggcga gcacccgcag ggccgtctgc acggccggcg
      541 cgctcagcgc gtgcgtgtcg gcgcccaaga agagggggcc cgcgatgccc tctttgttgc
      601 ggtaatcgac gatcgcctgg gtggtggcgg cgatgtgatc ctcgttgaac gatgtcgtca
      661 gcgagctgcc gcggtgcccc gaggtgccga agacgacccg ctcggccggt atcgacgggt
      721 cgggtctgcg ctcatggtag gcgtcgatca gcgcctcgat gtcgaccagg tcggagggtt
      781 cggccggggt tcctgcgcgc gggtccatca ccccagtctc ccatcctcgc taagcctggg
      841 gagagggccg cggggtggcg cgatgcgtcg tagccggccg gccggcgctc agccggcggg
      901 cgtcgtcacc cgcgcgtgcg tccagccgct cgtcgtcttg tggtaggcga cgcgcacgtg
      961 cttgcggtcg agcctggcct gcaggaactc gaactcggtg gcctgcaagg cgtatacctg
     1021 ccatgccgga ttcggcgcgc cggcgtaggc cggccgggtg cgccaatcgg cctgcgaggt
     1081 ctcgtcgtcg agcggcacca ccgtgcccgt gatgcgcacc tgcctgccgg attcccgcca
     1141 gaagaagagc atcgacgcac gcgggttcgc ggccacctga agacccttct tcgagtcgcg
     1201 gtgagtggag aaccagtagc cgcgctcgtc gcggtctttg aggatcacca tgcgggtgac
     1261 gggagttccc tctgctgaaa gcgtcgccag cgacagggcg ttcggctggc gggctccgct
     1321 tgcgatggcg tcgtcgagcc agccgaggaa cagccggtcc gggtcgtcgg gcacggcctc
     1381 cgtctcgagc gcaggcatct ggtcgggaaa actcggcacg gcacgcagcc ggtcacgaat
     1441 cgattccata ccgtcaccct acgttcgcgt ggccccgcgt ggcctcgtgc ggccgagccc
     1501 cgtgccggag ggccaggccg cggcatggcg ggcgtgggcg cttcgggccg cccgccagct
     1561 cccgtagcct gagtgcatgc agacccaacc ggcgggcgag acctacagct tcctcggccc
     1621 gagcggaacg ttcaccgagg cggcactcgc gcaggtgccg gaggcgcgcg gaaaggcgtg
     1681 gcggcccgtc aacaacgtgc cggaggcgct cgacgacgtc gtcaccgggc aaagcgtcgc
     1741 cgcgatgatc gcgatagaga actcgatcga gggcggggtc tccgtcgcgc aagacgcgct
     1801 cgccaccacg ccgggcctcc gaatcgtcgg cgaatacctc gtgccggtca gcttcatcct
     1861 cgtggccagg cccggcaccc ggctggccga cgtgcgcacc gtcaacgccc accccgtggc
     1921 ctacgcgcag tgtcaccgtt ggctgaatcg cacgctgccg cgccacgtgc acgtgcccgc
     1981 gaccagcaac gtgaccgccg cctccgccct gctcgagagc gatgcaccgg gcggtccgag
     2041 cgacgcggcg atcgccccgc cgacgatcgt cgaacaccac gacctcgacg tgctcgccac
     2101 cggcatcggc gacgacagca acgccatgac ccgcttcgtg ctcgtcggcc gcccgccgcg
     2161 cctgcccgca ccgaccggcg cagacaagac gagtctcatc gccgaactgc cggaggagcg
     2221 ggccggcacg ctcctcacca tgctgcaaca gttttctacg cggggcgtca acatgagcct
     2281 catcgagtct cggcccgtgg gcgacgcgct cggccgctac cgcttcgtca tcgaccttga
     2341 cgggcacatc cgcgacgaac gggtcgccga cgccctgctc ggcctgcacc ggttcagccc
     2401 gaacgtgatc tttttgggct cgtatccgcg ggccgaccgc cagccggtgc agttcgatcc
     2461 gcgataccgc gacgaggtgt tcacggccgc acgcacctgg ctggggggca tcatcgacgg
     2521 gggcgccgag gcgaggcggc ccttctgacg ccggtcagcg caggcgtcgg ggtgtcttta
     2581 tgacgagagc ccccggctcc acgcgaaagt gcagttcgcg ggcgatgccg gccggatccc
     2641 cgtcgacctc gaacggctgg ggttcgcggg aggtcacggt gacctccttg ccagccaggt
     2701 aggtgaccgt gcgccgccga cccggtctcg tgacgtgcag gagggcgccg gtgaagcgga
     2761 tcaggctgcg ccccagggcg ctgcgccgca acacccggtt ctcccatgtc acccgccgcc
     2821 agacgagcag ccagccgaag atgctcctcg gctgcagcac ggcgacgtcg agcagaccgt
     2881 cgtcgacggc cgcgtcgggg aacaggtcga tgccacccgg cagcctgcca ctgttcgcga
     2941 cgaggatcgt gctgatgcgg gccccgtgcg gggtgccgcc gtcgactgcg aagcgcaggt
     3001 gcatcggctt cagcgtgacg agcgagcgca ttccgccgtc gacgtacgcc agccagccca
     3061 ccctcttctt cagagacgac cgggtgttcg cgaggagcgt cgcgtcgacc cccatgcccg
     3121 ccatgacgac gaagacgtgt tcgtgggcgc tttgggatgc ccgctcgggc agggcgcgca
     3181 cgacgccgac gtcgacgctt cgcggctcac ccgtgaacgc gagggccacg gctttctcga
     3241 tcgagttcaa cggtaggttc aagccccggg cgaggaggtt gccagtgccc gccggcacga
     3301 tggcgagcgg cacgcccgtg ccccgcacga taccggccac cgcgcgcacc gtgccgtcac
     3361 cgccggccgt gatgatgagg gccgcgccgg tggaaagcgc ctcgcgtgcg gcacgcgccc
     3421 cgggatcgga gacggtcgtc tcccgccata ccggtgccgc ccagccgtgc tcgcggctgg
     3481 agcgttcgac ggccgcgcgg agcctttcga tgttcacctt gacggggttc gcgacgacgg
     3541 cggcgaagcg cacctctgcc gggtccgcgt ctcccatccc gccacgctac agcccggatg
     3601 gccgcccata gactggcggg gtgatcgatc ctgtcgtctt acgcgagaat cctgacctga
     3661 tccgtgactc ccagcgtgcc cgcggcgcgt cggtcgacct cgtcgacctc gccatcgagg
     3721 ccgacgccgc ccgccgcggc gcgctcgccg ccttcgagtc gctgcgtgcc gagcagaagc
     3781 aactcggcaa gaaggtggcg aaggcggcga aagaggagaa ggccgggctg ctcgccgcgg
     3841 cgcaggatct cgccgcacag gtgaaggccg cctccggtga agccgacgtc gcggtggggc
     3901 gcttcgagtc ggcggtgcgc aggctcggca acgtcgtgct cgccggggtt cctgccggcg
     3961 gcgaggagaa cttcgtcacg ctgcgcaccg gcggtgtgaa gccccgcttc gacttcacgc
     4021 cccgcgacca cctggaactg ggcgagatgc tcggcgccat cgacatggcc cggggcgcga
     4081 aggtctccgg cgcccggttc tactttctgc gcggcatcgg ggcgcgtctc gacctcgccc
     4141 tcatgcgtct cgccctcgac acggcgctgg acgccggctt cacgccgatg atcaccccca
     4201 ccctcgtgaa gcccgagatc atgcagggca ccgggttcct cggtgaacat gccgacgagg
     4261 tctaccacct cgacatcgac gacctgtacc tgaccggcac gagcgaggtc gcgctcgccg
     4321 gctaccacgc cgacgagatc ctcgacttcg cccatgggcc gatccgttat gccggatggt
     4381 ccacctgtta ccggcgcgag gccggctccg gcggcaaaga cacccgcggc atcattcggg
     4441 tgcaccagtt caacaagctg gagatgttcg tttacacaag cccggaccag gccgcggccg
     4501 agcacgagcg gctcgtcggc tggcaagagg gtctgctcgc ctcgctcggc ctgcactacc
     4561 gcgtcatcga cgtcgctgcc ggcgacctcg gccagagtgc cgcccgcaaa tacgacatcg
     4621 aggcgtgggt gccgacgcaa gacacctacc gcgaactgac ctccacgtcc aactgcacca
     4681 cgttccaggc caggcggctg ggcacccggt accgcacgag cctgggggag acgacgccgg
     4741 tcgcgacgct caacggcacc ctcgcgacca cccggtggat cgtcgccctc ttggagacgc
     4801 accagcaggc ggacggctcc gtgcgggtgc ccgaggtgtt gcgcccctac ctcggtgtcg
     4861 acgtgatgac gcccgtcgag gcccgctgac gtggggcccg cgccacggcg caggctcgtc
     4921 gcgctcgacg tcgacggcac tatcctgaac gccgacgaca ccgtcagccg acccgtcgtc
     4981 gaggcgatcg cggcggcggc gcgggccggc cacgaggtga tgctcgcgac ggggcgcagc
     5041 tgggaggcgg cggagccggt catgcgcaag gcgggcatcc gctcccgcta cgtcgtgtgt
     5101 gcgaacgggg cgatcacgat gaaacgcgac cctcgctcgc cgggcggcta ccgtcggttc
     5161 cacatcgata ctttcgacgc gcacgaggtc ttggcgacga tccgcccgcg gttgcccgac
     5221 ggtcggttcc tcgtcgaaga cgcgacaggc tttcgtcgct acaccgaggc ggccggcgac
     5281 tggctgctgg agcgcgccga gaaagtgccg ttcgacaggc tcgccgaggt gccggcgacc
     5341 cgggtcgtcg tcgtctcgcc cggccacgac gaagaggact tctttcgcgt ggtcgagagc
     5401 atggggctgc accgggtgag ctactcgatc ggctggaccg cgtggctcga catctcgccc
     5461 gaaggcgtga cgaaggcgac ggcgctggag cgggtgcgtg agctgctcgg cttcgagcgg
     5521 cgcgacgtca tctgcgtcgg cgacggccgc aacgacatcg agatgctgcg ttgggcggcc
     5581 gaagaaggcc gcggggtggc gatggggcac gcctccgacg acgtggtcgc cgccgcgaac
     5641 gaggtcaccg gcagcatcga cgacgacgga gtcgcggcgt tgctcgccgc gatctgagcg
     5701 actcccgggt gtcgtccagc accctgccag ccggtcatgc cacaatgtgg cacgccgcac
     5761 ggcacgggcg aagacccaaa gcgccgcatc gatcgggtgt ggcaacgcgg ttcgctgaac
     5821 cggaaggatg gggatgagcg acgagcccgg cggcgagagg cggcgcggac gccggtctgc
     5881 gttgcacggc acgcttgcgc gccacggcag gctgccaagg tcggggccgt ggcgcagcat
     5941 cttacggttc gtcgccgcag gcgtcgccgt cgtcgtggtg agcgcgttct cgatcgccgc
     6001 gatcgcgacg tgggacgtcg cccatacgct cgagaagaac accgtgcatc tggtcgatgc
     6061 caaagggaac ccggtcaccc cggaggtcgg cacgatcgcc ggggccttca acgtgctgct
     6121 cgtcggcacc gacacgcggc aggggcaggg gcccggctac ggcggcctgc gagagagctc
     6181 cgggtcgggg ctgaacgacg tgaccatgct gctgcacgtc tctgccgacc acgaccacgc
     6241 cacggtcgtg agcctgccgc gcgacatgtt cgtgcccatc ccctcctgcc cgaggccggg
     6301 cggcggcttc tacagcgcaa tgagcaggca gaagatcaac acgacgctgc gctacggcgg
     6361 cctctcgtgc acggtcttga ccgtgcagca gctgaccggc atgacgatcc cttacgcggc
     6421 cgaggtgacg ttcgacgggg tcatcgccat gtctgacgcg gtcggcggcg tgacggtgtg
     6481 caccacgggc acgatcaacg acccccacac cggcctgcat ctggcggccg gcgagcacac
     6541 gctgaagggc aaacaggcgc tcgagttctt gcgcacccgg cacggggtgg gtgacggcag
     6601 cgacctcggc cgcatcagca accagcaggt gttcctctcc gcgatgctgc gtcagatgat
     6661 gcgggccggc acgttgacca acctgcccga ggtgtacggc cttgccaagg ccgccgccag
     6721 caatatgcgc ctgaccgact cgctgaacgt ctccaccctc gcctcgatgg cgatggcgtt
     6781 gaagggcgtc aaccttagca aggtcgcctt catccagtac ccgaacgcga cccttcccga
     6841 cggcagcggg gtgatcccgc tgccccaggc ggcggcgacc ctcgtgacgg cgctgcagca
     6901 gggcaagtct ttgacgatca cagggggcac gggcgtagcc ggcgtcgggt ccgtcgctca
     6961 gacgccgtcg ccgtcgggaa cggggtcggc ctctggcagt tccctgaccg gctccgccac
     7021 gggctcgccc accacgacgt cgcccggttc cccgggtacg tcgtcttcca cctccgcagc
     7081 cacagacggc acgacgggca ccggctcccc ggcggatgag gccgtggccc tccccgatac
     7141 ggtgcacggc cagaccgcag ccgagaagac ctgttcgcgc gcgtacgact tctagcgacg
     7201 gccggctcgc cggcgagctc ctggcaggcg tcgcggccgg gggcgccctg gggcgccctc
     7261 ggctcgtgga accaactgtg aggtgctgca cagcacgcgg tcagcctggg tggacagaat
     7321 cggcacggct gatggtctcg tcgactgtga tcgatcctcg gccggagggt gtggatgagc
     7381 atggtgggcg gcgaacgccg ccgtggtcgg cgttcgaccg cgacggccac gctggcgcgt
     7441 cacggccgcc tgaagcgtcg tggcccatgg cgcagcgtgc tcaggttcgt cgtcgcgggc
     7501 gttgcggtcg tcgcgatcag cgccgtctcc atcacggcca tcacggtgtg ggatctctcg
     7561 agcaccctca cgagccacac catccacctg gtggacgcga aaggcaagcc cgtcgtgccg
     7621 gcggtcgact cgttgaaggg cgccttcaac gtgctgctgg tcggcacaga cactcgcacc
     7681 ggccagggct cgggctacgg ggcgctcacc gagagcgagg gtgctgggca gaacgacgtg
     7741 acgatactct tgcacgtctc ggcgaaccac aaccgggcga cggttgtcag ctatccacgt
     7801 gacatgttcg tctctgtgcc gtcgtgcccg aggccgcagg gcgggttctc gccggcgcag
     7861 gtcggccgca agatcaacac gacgctcatc gatggcggcc tctcctgcac cgttctcacg
     7921 gtggagcagc tgaccggggt gacgatcccc tacgcggcgg aggtgacgtt cgacggggtc
     7981 atcgctatgt cggatgcggt cggcggcgtg ccggtatgcg tctcgggcac catcaacgat
     8041 tcccacacgc acctgcacct gacgtcgggt gagcacacgc tggtcggcaa ggaggccctg
     8101 caatttctgc gcacccggca cggggtgggc gacggcagcg acctgggccg catcagcaac
     8161 cagcaggtgt tcctctcctc cctgatgcgc accatcatgg cgcgcggcac cctcacgaac
     8221 atctcgaagg tgtacgggct cgcgaaggcg gccgccacga acatgcggct gagcgactcg
     8281 ttgaacatct cgaccctggc gtcgatggcc gtcgcgatgc ggcacatcga cctcagcaag
     8341 gtgacgttcg tgcagtatcc gacgacgatc gtgccgggcg gcgtggatgc gctgcagccc
     8401 gccggcaacc tgctctcgca ggccattcag cacgaccagc cgatcacatt gacgtcgggc
     8461 accggcgcgc ccagggtggg gtcggtcgtc aaggggtctt cgtcgtcctc gtcctccggt
     8521 gcgaaggcgt cgacgagtgg cgggtcgtcc acgggatctg cctcttcggg atcctccgcc
     8581 cctccggcct cgtcggcaaa gggcgcgtca cccgccccga ccccccaggc ctcggcgccc
     8641 ccgtcggttg tcctgccgca cggcgtctac gggcagacgg cgacggagca gacctgctcc
     8701 cgggcgttcg acttctgacc cgcggccggg cccgtgccgg acgacgctcc cgccgataga
     8761 ctctggtgcg acgcgcggtc acgcgggtcg ggagggctgt ccgagcggcc gaaggagccg
     8821 gtcttgaaaa
//
LOCUS       DASTNL010000084         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_803109, whole genome shotgun sequence.
ACCESSION   DASTNL010000084 DASTNL010000000
VERSION     DASTNL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803109"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFQ96_02775"
     CDS             <1..297
                     /locus_tag="VFQ96_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HEU0256744.1"
                     /translation="TGTFKAWQRGTVVRSWLLSLLVRALEQDPEFEHIEGFVNDSGEG
                     RWTVEEALANAVPVPTISASIFARFVSRQEDSPAMKAVAALRNQFGGHAVKAVD"
     gene            320..1456
                     /gene="recF"
                     /locus_tag="VFQ96_02780"
     CDS             320..1456
                     /gene="recF"
                     /locus_tag="VFQ96_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU0256745.1"
                     /translation="MRVTRLSLTDFRNYRTAEISLDPGVTAFVGANGQGKTNLVEAIG
                     FLGSLGSHRVSSNQPLIRDGADFAVVRGLVDEGGRAVLLEVQINRSGPNRARVNHSAV
                     KPRDMLDHVTAVVFAPEDLALVRGDPAGRRDFLDGYLVRSTPRFSAVIADYDRVLRQR
                     NSLLKSARSVRPGASHPARLTTLDIWDERLVDLGSRIIEARLDALAALAGPVRDAYRR
                     IAGTDQRPAIVMQSSVVGEAAGPLPAAQIGHVFLRLVRESRAKELDRGVTLTGPHRDD
                     VLLLLNDLPARNHASHGESWSLALALRLGEADRLRDRSGAAGGPIIILDDVFAELDRR
                     RGRALAGALTGYEQVLVTAAIDDDVPDILGARVVRVTNGTVEVS"
     gene            1456..1953
                     /locus_tag="VFQ96_02785"
     CDS             1456..1953
                     /locus_tag="VFQ96_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEU0256746.1"
                     /translation="MKAEDGDECLRFYLHMKDLMHGSAHRSRPSGRVRRASVGAATEP
                     FSAGRDPRGLGEALDQLSVDLGWESSLAEAALLAGWRDLVGEETAAHTEPVSIERGVL
                     AVRCTSTAWATQLRLLRQDLLNRIDQRFPRAGIEMIRFEGPDVPSWKRGLRAVPGRGP
                     RDTYG"
     gene            2159..>3021
                     /locus_tag="VFQ96_02790"
     CDS             2159..>3021
                     /locus_tag="VFQ96_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0256747.1"
                     /translation="MHTNHSYTASDIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEI
                     VDNSVDEALAGYCDTIAVTIHPDGSVMVVDNGRGIPVAPHPVEKVSTVQVVLTKLHAG
                     GKFGGGGYAVSGGLHGVGSSVVNALSHRFDVEVKRNGYVWTMTFRDGVPVAPLKRGEP
                     STETGTTIRFWPNGEIFETTEFDYETLRTRFQQMAFLNKGLRIGLRDLRPGAEGDEGG
                     GRSDDFRYENGLRDYVHYLNSTKRSEVVHDEIISFESEDHDRGIALEVAMQWTTSYSE
                     SVYTYANTINT"
BASE COUNT          507 a          987 c         1013 g          514 t
ORIGIN      
        1 accggaacat tcaaggcctg gcagcgcggc actgtcgtgc gctcatggct tctgagcctc
       61 ctggtgcggg ctctcgaaca agaccccgag ttcgagcaca tcgaaggttt cgtcaacgat
      121 tccggggaag gccggtggac ggtagaagag gcgctggcga acgcggttcc ggtgccgacc
      181 atcagcgcct cgatcttcgc ccggttcgtc tcgcggcaag aggactctcc cgcgatgaaa
      241 gcggtcgcgg cgttgcgcaa ccagttcggc gggcacgcgg tgaaggccgt cgactgacgg
      301 aaccgaagga aggccctgga tgcgggtgac gcgactctcg ctcaccgact ttcgcaacta
      361 ccgcaccgcg gagatctccc tcgaccccgg tgtgactgcg ttcgtcggag cgaacgggca
      421 gggcaagacc aacctcgtcg aggcgatagg attcctcggc tcgttgggct cgcaccgcgt
      481 ctcctccaac cagccgctca tccgagacgg cgccgacttt gccgtcgtgc gcggcctcgt
      541 cgacgagggc ggtcgcgccg ttctcctcga ggtgcagatc aatcgctcgg ggcccaatcg
      601 ggcccgggtg aatcactccg cggtgaagcc ccgcgacatg ctcgaccacg tgaccgcggt
      661 cgtcttcgca ccggaagatc tcgccctggt acgcggtgac ccggccggcc gacgggattt
      721 tctcgacggc tatctcgttc ggagcacccc tcgcttctcg gccgtcatcg ccgactacga
      781 ccgtgttctg cggcaacgaa actctctctt gaagtcggcc cggtcggtgc gccccggcgc
      841 aagtcatccc gcccgcttga cgacgctcga catctgggat gaacggctcg ttgacctcgg
      901 cagccgcatc atcgaggccc ggctggatgc gctcgcggcg ctcgcaggcc ccgtccgcga
      961 cgcctatcgc cggatcgccg gaaccgacca acgaccggcg atcgtgatgc aatcctccgt
     1021 tgtcggcgag gcggccggcc ccctgccggc ggcacagatc ggccacgtct ttcttcgact
     1081 ggtgcgggag tcccgggcga aagagctcga tcgaggcgtg acgttgacgg gcccgcaccg
     1141 cgacgacgtg ctccttctct tgaacgactt accggcgagg aaccacgcca gccacgggga
     1201 atcttggtcg ctcgcgctgg ccctgcgcct gggcgaggcc gaccggcttc gcgaccggtc
     1261 gggcgccgcc ggcgggccga tcatcattct cgatgacgtg ttcgccgaac tggatcggcg
     1321 ccgcggccgt gcgctggcgg gcgctttgac gggttacgag caggttctcg tgacggccgc
     1381 gatcgacgac gacgtgcccg acatcctggg tgcgcgtgtc gtccgggtca cgaacggcac
     1441 ggtggaggtc tcgtgatgaa ggcggaggac ggcgacgagt gccttcggtt ctaccttcac
     1501 atgaaggacc tcatgcacgg ctcggcccac cgctcgcggc cgtctggtcg cgtgcgtcgg
     1561 gcgagcgtcg gggcggccac ggaaccgttt tccgccggcc gcgacccgcg agggctcggc
     1621 gaggcgctcg accagttgag tgtggatctt ggctgggagt catcgttggc cgaagcagct
     1681 ctcttggcag gctggcgtga cctggtcggt gaggagacgg cggcgcacac tgagccggtc
     1741 tcgatcgaac ggggcgtgct cgcggtgcgc tgcacgtcga cggcctgggc gacacagctg
     1801 cgtctgttgc gccaagacct cctgaaccgc atcgatcagc gtttccctcg ggcgggtatc
     1861 gagatgatcc ggtttgaggg accggacgtc ccttcgtgga aacgtggcct cagagccgtt
     1921 ccagggcgtg gaccacgcga tacctacggg tgacgagtca aaaaactccg gtgatgtggg
     1981 ttcgtgcctt cagcaggccg tatgggaggt ttctgggccg ccttcttgat agactggggt
     2041 cgtcatcgat cacatggtca ggagccgcac ccctatgaca ttggaacccg ccgacgcccg
     2101 ggacgcgaat ccctcccacg aagaatcgca ccacacggac gacgccgatg ttctggggat
     2161 gcataccaac cacagctaca ccgccagcga catccaggtt ttggaagggc tcgaggcggt
     2221 gcgcaagcgc ccgggcatgt acatcggctc aaccgggccg cgcggtctgc accacctcgt
     2281 ctacgagatc gtcgacaatt cggtcgacga ggcgctcgcc ggttactgcg acacgatagc
     2341 cgtcacgatc caccccgatg gcagcgtcat ggtcgtcgat aacggccgcg gcatacccgt
     2401 ggccccgcac ccggtcgaga aggtctccac cgttcaggtg gtgctcacca agctgcacgc
     2461 cggaggcaag ttcgggggcg gtggatacgc ggtgtcgggc ggcctgcacg gtgtcgggtc
     2521 gtcggtcgtc aacgcgctct cacaccggtt cgacgtcgag gtgaagcgca acggttatgt
     2581 ctggacgatg accttccgcg acggagtgcc ggtcgcgccg ttgaagcggg gcgagccgag
     2641 caccgagacg ggcacgacga tccgcttctg gccgaacggc gagatcttcg agacgacgga
     2701 gttcgactac gagacgctgc gcacgcggtt tcagcagatg gcgtttctca acaaggggct
     2761 gcgtatcggg ctgcgagatc tgcggcccgg ggccgagggc gatgagggcg gcggccgcag
     2821 tgacgatttc cgttacgaga acggcctgcg cgactacgtg cactacctca acagcacgaa
     2881 acgttccgag gtcgtgcacg acgagatcat ctcgttcgag tcggaagacc acgaccgcgg
     2941 catcgcgctc gaggtggcga tgcagtggac gacctcatac tccgagagcg tctacaccta
     3001 cgccaacacc atcaacaccc a
//
LOCUS       DASTNL010000085         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_434755, whole genome shotgun sequence.
ACCESSION   DASTNL010000085 DASTNL010000000
VERSION     DASTNL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434755"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFQ96_02795"
     CDS             <1..356
                     /locus_tag="VFQ96_02795"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244474.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU0256748.1"
                     /translation="YFEVLHEMKLIVDLINEGGIAKQRWSVSDTAEYGDYVSGPRVID
                     ARVKENMKAVLSDIQDGTFAKRFIADQDAGAPEFLKLRAEGEGHPIEETGRKLRGLFA
                     WNSVQDDYTDGQVAR"
     gene            630..2222
                     /gene="serA"
                     /locus_tag="VFQ96_02800"
     CDS             630..2222
                     /gene="serA"
                     /locus_tag="VFQ96_02800"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU0256749.1"
                     /translation="MSKPVVLIADQLSPATVEALGPDFEVRHVDGTDRSALLAALGEA
                     NAVLIRSATKIDAEAVAAAPNLTIIARAGVGLDNVDIKAATAAGVMVVNAPTSNIISA
                     AELTVGHMLGLARNIAAANTSLTGGAWKRSSFTGTEMYEKTIGIIGLGRIGALVTARM
                     QAFGTTVVAYDPYVTSARAQQLGVRLVGLDELLSVSDFITIHMPKTPETLGMIGHDQL
                     AKMKPSAFILNVARGGLIDEEALYDALSNRRIAGAAVDVWSSEPPTGSPLLGLGNIVV
                     TPHLGASTHEAQEKAGVSVARSVRLALAGELVPDAVNVAGGVIDEYVRPGIPLVEKLG
                     QVFSGLVTSALTSIDVEVHGELVDYDVSVLRLAALKGVFTNIVSESVSYVNAPVLAEQ
                     RGITVRLLTDAVSADYRNVITLRGALSDGTQISVSGTLTGPKQIEKIVGIDGYDVEVP
                     IARHHIVLKYVDRPGIVAVFGNELGRAGVNIAGMQIARTAAGGEALSVLTVDSPVPEG
                     VLAAVRDAIDAHTLREIDITED"
     gene            2378..3445
                     /locus_tag="VFQ96_02805"
     CDS             2378..3445
                     /locus_tag="VFQ96_02805"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU0256750.1"
                     /translation="MSRTVRLAAIPGDGIGPEVVAEALKVLSAATAADDVAFETTRFS
                     LGAARYLETGDILTEDDLARIAGHDVILLGAVGGKPDDPRLKNVGIERKLLLTLRFRL
                     DQYVNLRPTRLFPNVASPLKDPGDVDFVVVREGTEGPYVGTGGSIRTGTPHEIANEVS
                     VNTAFGVERVVRYAFGQAAARRRKLTLVHKTNVLTFAGALWRRIVERVGADYPDVEVD
                     YLHVDAATIFLVTQPARFDVIVTDNLFGDILTDLAGAVSGGIGLAASGNVNPEGRFPS
                     MFEPVHGSAPDIAGRQIADPTAAILSVALMLHHLGLPDAAARVEGAVTADLASRGDEA
                     RTTRQVGDAIAAACQPATADN"
     gene            3464..4570
                     /locus_tag="VFQ96_02810"
     CDS             3464..4570
                     /locus_tag="VFQ96_02810"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU0256751.1"
                     /translation="MTITDPGTQPATFALTRNPHPTPEARRSAILADPGFGNYFTDHM
                     VSVTWTPETGWANAAVTPYGPLQLDPAAAVLHYAQEIFEGMKAYRHADGSIHTFRPEA
                     NAARFRRSAHRLALPEMPEDLFVRSLKELIAVDGAWVPDAPEMSLYLRPFMFAEEAFL
                     GVRPAKRVAYHLIASPAGAYFAGGVEAVKIWLSTDYARAGKGGTGAAKTGGNYAASLL
                     PQAEAYAEGCAQVVFLDSVEEKYLEELGGMNIVVVYKDGTLATPKSESILAGITRESV
                     LQLAVDEGHPVEERRIAIDELKEGIASGEVTELFACGTAAIVTPISELKAKDFSLTPV
                     KPEGDLSLRLRAKLTDIQYGRAADTHQWLTRLDA"
BASE COUNT          785 a         1624 c         1528 g          711 t
ORIGIN      
        1 cgtacttcga ggtacttcac gagatgaagc tcatcgtcga cctgatcaac gagggcggca
       61 tcgccaaaca gcgttggagt gtctcggaca cggcggagta cggcgactac gtctccggcc
      121 cgcgcgtcat cgacgcccgc gtgaaggaga acatgaaggc ggtgctctcc gacattcaag
      181 acggcacctt cgcaaagcgc ttcattgccg accaagacgc gggcgcgccg gagttcttga
      241 agcttcgtgc cgagggtgaa ggccacccga tcgaggagac cgggcgcaag ctgcgcgggc
      301 tcttcgcctg gaacagtgtg caagacgact acaccgacgg tcaagtcgcg cgctgagaag
      361 cagaagtgag gcggtgcggc accctggggt gctgcaccgc ctttgcgtct gcctgctgag
      421 ccgcttcggg tgagagcacc cggccggccc gcatcgaggc cgcctcgccg cggcagcggc
      481 ccagggagcc ttggccggtg cgtccttcgg cctgttttcg gcggcctggt tttatcggcg
      541 ctcgccccac acgatagcct ggaagccacg tggcgcccca aaggcggtgg cgcacgcgac
      601 cgtcgagacc agagagaagg aacagccacg tgtccaagcc cgtcgtgttg atcgccgacc
      661 agctctcacc cgccaccgtc gaggccctcg gcccagattt cgaggtgcgc cacgtcgatg
      721 gcaccgaccg ttcggcgctt ctcgcggcgc tcggcgaggc caacgccgtc cttatccgtt
      781 ccgcgacgaa gatcgacgcg gaggcggtgg ccgcggcccc gaacctcacg atcatcgcca
      841 gggcgggcgt ggggctcgac aacgtcgaca tcaaggccgc cactgcggcc ggcgtcatgg
      901 tcgtgaacgc gccgacctcg aacatcatct ccgccgcgga gttgacggtc ggccacatgc
      961 tggggctcgc ccgcaacatc gcggcggcca atacgtctct cacaggcggc gcgtggaagc
     1021 gctccagctt caccggcacc gagatgtatg agaagacgat cggcataata ggcctcggcc
     1081 gcatcggcgc cctcgtcacc gcgcgcatgc aggcgttcgg caccaccgtc gtcgcctacg
     1141 acccgtatgt gacgagtgcg cgggcgcagc aactcggggt gcgcctcgtc gggctcgacg
     1201 agctgctctc ggtcagcgac ttcatcacga tccacatgcc gaagaccccg gagacgctcg
     1261 gcatgatcgg ccacgatcag ctcgcaaaaa tgaagccgag cgcgttcatc ctgaacgtcg
     1321 cccgcggcgg cctcatcgac gaggaggcgc tctacgacgc cctctcgaat cgccggatcg
     1381 cgggtgcggc ggtggacgtc tggtctagcg agccgccgac cggttctcct cttctcgggc
     1441 tcggcaacat cgtcgtcacg ccgcacctcg gcgcctccac ccacgaggcg caggagaagg
     1501 ctggggtctc ggtggcgaga tctgtgcgcc tggccctggc cggcgagctc gtgccggacg
     1561 ccgtgaacgt ggcgggcggc gtcatcgacg agtacgtgcg gcccggaatc ccgttggtag
     1621 agaaactcgg ccaggtcttc agcgggctcg tgacgagtgc gcttacgagc atcgacgtcg
     1681 aggtgcacgg cgagctcgtc gactacgatg tgagcgtctt gcggctcgcc gcgttgaagg
     1741 gcgtgttcac caacatcgtc agtgagtctg tctcgtacgt caacgctccg gtgctcgcag
     1801 aacagcgcgg catcacggtg cggctcttga ccgacgcggt gagcgccgac taccgcaacg
     1861 tcatcacgct gcgcggcgcc ttgagcgacg gcacccagat ctcggtgtcc ggcaccttga
     1921 cggggccgaa gcagatcgag aagatcgtcg gcatcgacgg ctacgacgtc gaggtgccga
     1981 tcgcgcgtca tcacatcgtg ctgaagtatg tggacaggcc cggtatcgtc gccgtcttcg
     2041 gcaacgaact cggccgagct ggcgtcaaca tcgccggcat gcagatcgcc cgtacggccg
     2101 ccgggggaga ggctctcagc gtcttgaccg tcgactcccc ggtgccggag ggtgtgctcg
     2161 ccgccgtgcg tgacgccatt gacgcccaca cgctgcggga aatcgacatc accgaagact
     2221 gaggccgcgc tcggccggcc ggcccgggat gcctcgtgcg cgagggtact tgccgaggcg
     2281 ctgtgctgcg cccggcggcg tgccgggggc cggcagcgga cgagtacgct gagatcgtct
     2341 cccacccatc caggcgtcgt attcgctaag gagcctcatg tcgcgcaccg tcagactggc
     2401 cgcaataccg ggcgacggca tcggccccga ggtcgtcgcc gaggcactca aggtgctgag
     2461 tgcggcgacg gcggccgacg acgtggcctt cgagacgacc cgcttctcgc tcggcgccgc
     2521 acgttacctc gagacgggcg atatcctcac cgaagacgac ctggcccgga tcgccggcca
     2581 cgacgtgatc ctcctcggcg cggtcggcgg caaacccgac gacccgcgcc tgaagaacgt
     2641 gggcatcgag cgcaaactgc tcctgaccct ccgcttccgg ctcgaccagt acgtcaacct
     2701 gcgacccacc aggctgttcc cgaacgtggc gagccccttg aaagacccgg gcgacgtcga
     2761 cttcgtcgtc gtgcgagagg gtacggaggg gccgtatgtc ggcaccggcg ggagcatccg
     2821 caccggtacg ccgcacgaga tcgccaacga ggtctctgtg aacaccgcat tcggcgtgga
     2881 acgcgtggtg cgttacgcct tcgggcaggc cgctgcccgc cgccgcaagc tgacgctcgt
     2941 gcacaagacg aacgtgctca ctttcgcggg ggccttgtgg cggcgcatcg tcgagcgggt
     3001 cggcgccgac tatcccgacg tcgaggtcga ctacctgcac gtcgatgcgg cgacgatctt
     3061 cctcgtcacc cagccggcac gcttcgacgt gatcgtcacc gacaacctct tcggcgacat
     3121 tctcaccgac ctggcgggcg ccgtcagcgg cggcatcggc ctcgccgcct ccggcaacgt
     3181 gaaccccgag ggccggttcc ccagcatgtt cgagccggtg cacggctccg cacccgacat
     3241 cgcggggcgt cagatcgccg atccgaccgc ggcgatcctc tccgtcgcct tgatgctcca
     3301 ccacctgggc ctgcccgacg cggccgcccg tgtcgaaggc gcggtcaccg ccgacctcgc
     3361 gtcccggggc gacgaggccc gcaccacccg tcaggtcggc gacgccatcg ccgcggcctg
     3421 ccagccggcc acggccgaca actgagaaca cgaaggatcc tctatgacca tcaccgaccc
     3481 aggcacccag ccggccacat tcgcgctcac gcgtaacccc cacccgactc ccgaggcacg
     3541 ccgcagcgcg atactcgccg accccgggtt cggtaactat ttcaccgacc acatggtcag
     3601 cgtcacgtgg accccggaga ccggatgggc gaacgccgct gtcacgccgt acggaccgtt
     3661 gcaactcgac ccggccgccg ccgtcttgca ctacgcgcaa gagatcttcg aaggcatgaa
     3721 ggcttaccgc cacgccgatg gctcgatcca caccttccgg ccggaagcca acgccgcccg
     3781 ctttcggcgc tccgcccaca ggctcgcgct gcccgagatg cccgaagacc tcttcgtgcg
     3841 ctccttgaaa gagctgatcg cggtcgacgg cgcatgggtt cccgacgcac ccgagatgag
     3901 cctgtatctt cggccgttca tgttcgccga agaggcgttc ctcggtgtgc ggcccgcgaa
     3961 acgggtggcc taccacctca ttgcgagccc cgccggcgcc tatttcgccg gcggcgtcga
     4021 ggccgtcaag atctggctct ccaccgacta cgcccgcgcc ggcaagggtg gcaccggggc
     4081 ggcgaagacc ggcggaaact atgctgccag cctgctgccg caggcggagg cctacgccga
     4141 gggctgcgct caggtggtgt ttctcgactc ggtggaggag aagtacctcg aagagctcgg
     4201 cggtatgaac atcgtcgtcg tttacaaaga cggcacgctc gccacgccga agtccgagag
     4261 cattctggcc ggcatcaccc gggagtcggt gctacagctg gcggtcgacg aagggcatcc
     4321 ggtcgaggaa cgtcgcatcg cgatcgacga gttgaaagag gggattgcct cgggcgaggt
     4381 gacggagctc ttcgcctgcg gcaccgccgc catcgtgacc ccgatcagcg agctcaaggc
     4441 caaagacttc agcctcaccc cggtgaagcc ggagggtgat ctcagcctgc ggctgcgtgc
     4501 gaagctcacc gacatccagt acggccgcgc cgccgacacc caccagtggt tgacgaggct
     4561 cgacgcatga gcgccgcttg ccagacaagc ggcccgggcg cgggggcgcg tcggtcagcg
     4621 tcgccagaac gtcgggggag gcacgggc
//
LOCUS       DASTNL010000086         6788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_66057, whole genome shotgun sequence.
ACCESSION   DASTNL010000086 DASTNL010000000
VERSION     DASTNL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66057"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="prs"
                     /locus_tag="VFQ96_02815"
     CDS             complement(<1..393)
                     /gene="prs"
                     /locus_tag="VFQ96_02815"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU0256752.1"
                     /translation="MSGIKANGQKRLVLISGRAHPQLAQQIADTLGSELVPTDTRTFA
                     SSEIYSRFDESVRGSDAFVIQSHTSPINEWLMEQLIMVDALKRASAKRITVVAPYYPY
                     ARQDKKGRGREPISARLVADLFKVAGADR"
     gene            complement(394..1818)
                     /gene="glmU"
                     /locus_tag="VFQ96_02820"
     CDS             complement(394..1818)
                     /gene="glmU"
                     /locus_tag="VFQ96_02820"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU0256753.1"
                     /translation="MPDERLAVIILAAGRGTRMKSAIPKLLHPLGGVPMISHVLASAR
                     ELDAARVLVVVRYERDQIARVVASELPDAVIVDQDEIPGTGRAVEQAVAALPAGFDGD
                     VLVVNGDVPLLNAATLRGVVETHRASGAPMTILTTNLDEPDGYGRVLRAGDGGVERVV
                     EHADAGVEELVVTEINAGVYLFQAQALREGLPAIGVENAQGEKYLTDLVGVLHAAGAR
                     VEAKRVTPSWLVAGINNRAQLGEVARRLNAMIVRGWQLAGVTVQDPATTWIDLRVTLA
                     PDVTILPGTQLHGTTSIATGATIGPDTTLVDCRVGQGASVRRTDATEAVIEAEAKVGP
                     FAYLRPGTRLAAGGKIGTFVETKNAVIGRGSKVPHLSYVGDATVGEGANIGAATIFAN
                     YDGVNKHASKVGSHVRTGSHNVFVAPVEIGDGAYTGAGTVVRRDVPAGALAINEAPQR
                     NIEGWVARKRPGTEAAKAAGEGDD"
     gene            complement(1884..1955)
                     /locus_tag="VFQ96_02825"
     tRNA            complement(1884..1955)
                     /locus_tag="VFQ96_02825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1921..1923),aa:Gln,seq:ttg)
     gene            2078..2608
                     /locus_tag="VFQ96_02830"
     CDS             2078..2608
                     /locus_tag="VFQ96_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0256754.1"
                     /translation="MPEASDEVDEIVAAWSRERPELDFSPLQVFSRLDRLSKLADHNR
                     RLAFELSGLEPWEFDVLSALSRSGEPYVMSPKSLLRQTFVSSGTMTNRIDRLVARGLV
                     ERRTDPHDRRGVLVHMTREGLSRADAAIGALVEREREALEGLSPADRRALAGLLRRLG
                     NDLRPTGASRPRAARR"
     gene            complement(2686..3345)
                     /locus_tag="VFQ96_02835"
     CDS             complement(2686..3345)
                     /locus_tag="VFQ96_02835"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEU0256755.1"
                     /translation="MSDFLANLPLFFQSVWETIYIVGIALIVSGFLGLALGVGLTVTR
                     PGHLLDNRAVYMVINVVVNLVRPIPFVLFITAIGPVTILAVHTTVGNAAVVFALSIAA
                     CFAVARIVEQNLVTVEPGVIEAARAMGAGPWRIIATVLIPEGLGPLVLGYTFAFVGIV
                     DMSAQAMLIGGGGLGSFAIVYGYQRYNWPIVYISVAVIVVLVQAGQFFGNHLARRALR
                     R"
     gene            complement(3345..4412)
                     /locus_tag="VFQ96_02840"
     CDS             complement(3345..4412)
                     /locus_tag="VFQ96_02840"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU0256756.1"
                     /translation="MSALVRLEHVTKTFVTPSRSVTAVDDVSLDIEQGGILGVIGYSG
                     AGKSTLVRLINALEQPTAGAVTVGGVRLTGLPERALREERRQIGMIFQQFNLFHSRTV
                     AGNVAYPLQVAGVPKGRHDARVAELLDFVGLLDRAHSYPEQLSGGQKQRVGIARALAT
                     SPRLLLADEATSALDPDTTAEVLALLDRINRELGVTIVVITHEMEAVRAIAHQVAVMD
                     SGRIVEAGDVYDVFATPASPVTARFVSSVLRDRPSAHTVERLRSSHPGRIVSVRVGEE
                     RGFLGMVQGVFAEHGVTPELVYGGISELRDRTIGSLTYELTGAAADVASSVAALRGRG
                     CEVVDEASAGDGGSRRVSAGA"
     gene            complement(4453..5433)
                     /locus_tag="VFQ96_02845"
     CDS             complement(4453..5433)
                     /locus_tag="VFQ96_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0256757.1"
                     /translation="MSDSQNGQNPPRQAPKLPEKATKKRGRKTLGWVIVAVVVAAAIT
                     VTGIVVGNRTTGETAKASAKTVSIGVTDAAQPYWKTFSRLAAKELDVKVKIVNFTDYS
                     QPNPALSQGQLDLNEFQHIEFLADYNVSAHDDLQPIGATAVYPLPLYSTKYTSVSALP
                     KGGTVALPNDAINEARGLLVLQSAKLLTLKDGGTPFSTASDIVSSKVKVLPVDAAQTA
                     VALKNGSAVAAIVNNNFAVSAGLPHKDAIFTDDPASPSAKPYVNVFVARAADKNNALY
                     KKLAALYQNPAVKAGVQKSNGGTAVFRTTSAKELQAELAKVEAQERATKK"
     gene            complement(5644..>6788)
                     /locus_tag="VFQ96_02850"
     CDS             complement(5644..>6788)
                     /locus_tag="VFQ96_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256758.1"
                     /translation="LAGSFPALLIGRMVQACGTAVMIPLVMTTVMRLVAPGRRGTVMG
                     TISIVIGVAPAVGPTVGGAILAGLGWRWMFWLVLILAAVMLALALLWLHVPGTTRAVP
                     LDAVSVVLSALGFAGLVYGLSTLGQEGQAVPPWLALPVGALALAGFAWRQARLQRRDR
                     PLLDLRTLAVPRYLIALILSMLVFMALLGAGAVLLPIYLQHVLGHGVFVAGLALLPGG
                     VMMAAVSRPVGALYDRVGAKALVIPGAAGMSVALFLLALLGSAAPLAAVIGIDMLMMS
                     SLGVMMTPLMADSLGALTDDLYSHGSALLTTLQQVAGALGSAVFVAVAALGGVDSSGL
                     PGASGIRLAFVVAGLVGVAAFGVALAYPRGKPEGVGSAGGPGIAHA"
BASE COUNT         1086 a         2305 c         2380 g         1017 t
ORIGIN      
        1 gcggtccgcc cccgccactt tgaagagatc ggcgacgaga cgggccgaga tgggctcgcg
       61 gccgcggccc ttcttgtctt gtcgcgcata tggatagtag ggcgcgacga cggtgatgcg
      121 tttcgcggag gcccgcttca acgcatcgac catgatgagc tgctccatga gccactcgtt
      181 gatgggggac gtgtgcgact ggatgacgaa cgcgtcggag ccccgcacgc tctcatcgaa
      241 acgagagtag atctcgctgc tcgcgaacgt tcgcgtgtcg gtcgggacga gctcactgcc
      301 gagggtgtcg gcgatctgct gggccagctg agggtgcgcg cgccccgaga tgagcacgag
      361 gcgtttctgc ccgttggcct tgatacccga cattcagtcg tcgccctccc ccgcggcctt
      421 ggccgcctcg gtgccggggc gtttgcgcgc cacccagcct tcgatgttgc gttgcggtgc
      481 ctcgttgatc gccaaggcgc cggcaggcac gtcacggcgc acgacggtgc ccgccccggt
      541 gtatgccccg tcgccgatct cgaccggggc gacgaagacg ttatgcgacc cggtgcgcac
      601 gtgtgagccc accttcgagg cgtgcttgtt gacgccgtcg tagttggcga agatcgtggc
      661 ggcgcctatg ttggcgccct cccccaccgt cgcgtcgccc acatagctca agtgcggaac
      721 cttcgaaccg cggcctatga cggcgttctt cgtctcgacg aacgtgccga tcttgccgcc
      781 ggcggccagc ctggtgccgg gcctcaagta agcgaacggc ccgaccttcg cctccgcttc
      841 gatgacggcc tcggtcgcgt cggtgcgcct cacgctcgca ccctgcccca cccggcaatc
      901 gacgagggtc gtgtcggggc cgatcgtcgc gccggtggcg atgctcgtcg tgccgtgcag
      961 ttgggtgccg ggcaggatcg tcacgtcggg cgcgagggtg acgcgcaggt cgatccaggt
     1021 ggtggcgggg tcttgcacgg tgacgccggc cagctgccaa ccgcgcacga tcatcgcgtt
     1081 cagccgccga gccacctcgc cgagctgagc ccggttgttg atgccggcga cgagccacga
     1141 gggcgtcacg cgcttcgcct cgacccgggc gcccgcagca tgcagcacgc cgacgaggtc
     1201 ggtcaggtac ttctcaccct gcgcgttctc gacgccgatc gcaggcaggc cctcccgcaa
     1261 cgcctgtgcc tggaagagat acaccccggc gttgatctcg gtcacgacga gctcttccac
     1321 gcccgcgtcg gcgtgttcga cgacgcgttc gaccccgccg tcgcccgccc gcagcacacg
     1381 accgtagccg tcgggctcgt cgagattcgt cgtgaggatc gtcatcggcg cccccgaggc
     1441 acggtgcgtc tcgacgacac cccgcagggt cgccgcgttc aggagcggca cgtcgccgtt
     1501 cacgacgagc acatctccgt cgaatccggc aggcaacgct gcgaccgcct gttcgacggc
     1561 tcggccggtg ccggggatct cgtcttggtc gacgatcacg gcgtcaggca gctcgcttgc
     1621 caccacgcgg gcgatctgat cgcgctcgta ccgcaccacg acaagcacgc gggcggcatc
     1681 caactcgcgg gcgctcgcga gcacgtgaga gatcatcggc acgccgccga gcgggtgcag
     1741 aagcttgggg atggccgact tcatgcgggt gccacggccg gcggcaagga tgatgacggc
     1801 gagcctctcg tcgggcatgg tccggcttct cccctctgtc tgagcgccgc cccacgcccg
     1861 ccctcaggac atcggctgcc tcgctccgcc tccaggattc gaacctagac ctaacagctc
     1921 caaaggctgt cgtgctgcca ttacaccaaa gcggaccacg ggcgcccatc cggatgccct
     1981 gcatccagtt tgccagacat cggcgaacgc ttcgaccgac ctaggcgtcg gccggaccgg
     2041 cccggggcac cacggcgaga cctggataat gaaaaccatg cctgaagcat cggacgaggt
     2101 cgacgagatc gtcgccgcct ggagcagaga acgccccgag ctcgactttt ccccccttca
     2161 ggtgttctcg cgcctcgacc gcctctcgaa actggccgac cacaatagac gcctggcgtt
     2221 cgagctttcc gggctggagc catgggagtt cgacgtgctc tcggcactca gtcgctccgg
     2281 cgagccgtac gtcatgagcc ccaagtcgct tctgcggcag acgttcgtct cgagcggtac
     2341 catgacgaac cgcatcgacc ggctcgtcgc gcgcggcctc gtggaacgcc gtaccgaccc
     2401 ccacgaccgg cgcggcgttc tcgtccacat gacccgggag ggtttgagcc gcgccgacgc
     2461 cgcgatcggg gcgctggtcg agcgggaacg cgaggcgcta gaaggcctca gccccgccga
     2521 ccgccgcgcg ctcgcgggcc tcttgcggcg cctcggcaac gacctgcgcc cgacgggcgc
     2581 gagccggccc cgtgccgccc gacgctgacg gtgggccgcc gcgaggcggt cggcgccggc
     2641 gggcggatca cgccgcggcc cgggtaggcg gtgagacggg gtccgtcagc ggcgcagcgc
     2701 ccggcgggcg agatggttgc cgaagaactg gcccgcctgc accagcacga cgatgacggc
     2761 gaccgagatg taaacgatag gccagttgta gcgttggtag ccgtagacga tcgcgaagct
     2821 gccgagccca ccgccgccga tcaacatagc ctgcgccgac atgtcgacga tgccgacgaa
     2881 cgcgaatgta tagccgagca cgagcggccc caggccctcc gggatcagca cagtggcgat
     2941 gatccgccac ggcccggccc ccatcgcccg cgccgcctcg atgacgcccg gctccacggt
     3001 gacaaggttc tgctcgacga tgcgggccac cgcgaaacac gccgcgatgg agagcgcgaa
     3061 gacgacggcc gcgttgccga ccgtggtgtg cacggccagg atcgtgacgg gcccgatcgc
     3121 cgtgatgaag agcacgaacg ggatcggccg cacgaggttc acgaccacgt tgatcaccat
     3181 gtagacggcg cggttgtcga ggagatgccc cggccgggtg acggtcagcc cgacgcccag
     3241 cgcgaggccg aggaacccgc tcacgatgag ggcgatgccg acgatgtaga tcgtctccca
     3301 gacggactgg aagaagagcg gcaggttcgc gaggaaatcg ctcatcacgc gcccgccgaa
     3361 acccggcggg acccgccgtc gccggcggaa gcctcgtcga ccacctcgca gcccctgcca
     3421 cgcagggccg cgaccgagga ggcgacgtcg gccgcggcgc cggtcaactc gtaggtgagg
     3481 ctgccgatcg tccggtcgcg cagttcgctg atgccgccgt agacgagctc cggggtgact
     3541 ccgtgttccg cgaagacacc ctgcaccatg ccgagaaaac cccgctcctc ccccacccgc
     3601 acgctgacga tgcggcccgg atggctggag cgcagccttt ccaccgtgtg ggcggagggg
     3661 cggtcgcgca gcaccgagct gacgaaacgc gccgtgacgg gcgaggcggg ggtggcgaac
     3721 acgtcataga cgtcgcccgc ctcgacgatg cgtccggaat ccatgacagc gacctggtgc
     3781 gcgatggccc gcaccgcctc catctcgtgg gttatgacga cgatcgtgac gccgagctcc
     3841 cggttgatgc ggtcgagcag ggcgagaacc tcggccgtgg tgtcggggtc gagcgcgctc
     3901 gtcgcctcat cggccagaag caggcgcggg ctcgtcgcga gcgcccgcgc gatgccgacc
     3961 cgttgcttct gcccgccgga gagttgttcc gggtaactgt gggcccggtc taacaagccc
     4021 acgaagtcga gcaattcggc gacccgggca tcgtggcggc ccttcggcac cccggcgacc
     4081 tgcagcggat aggcgacgtt gccggcgacg gtgcgggagt ggaacaggtt gaactgctgg
     4141 aagatcatgc cgatctgccg gcgttcctcg cgtagcgcgc gctccggcag ccccgtcagc
     4201 cgcaccccgc cgaccgtgac ggcgccggcg gtcggctgtt ccagcgcgtt gatgagcctc
     4261 acgagcgtgc tcttgcccgc gcccgaatag ccgatgacgc cgaggatgcc gccctgctcg
     4321 atgtcgaggc tgacatcgtc gacagccgtc acagagcggc tgggggtgac gaacgtcttc
     4381 gtcacgtgtt ctagccgaac gagggcgctc atcgcttcgt tccgctccgt tcccgatgct
     4441 ggggccgtca gactacttct tggtcgccct ctcctgggcc tccactttag ccaactcggc
     4501 ctggagttct ttcgccgagg tcgtacggaa cacggcggtt ccgccgttgg acttctgcac
     4561 gcccgccttg acggccgggt tctggtagag agccgcgagc ttcttgtaga gggcgttgtt
     4621 cttgtcggcg gcacgagcga cgaacacgtt gacgtacggc ttcgcgctgg ggctggccgg
     4681 gtcgtcggtg aagatcgcgt ccttgtgggg aaggcccgcg gagacggcga agttgttgtt
     4741 gacgatcgcc gcgacggcag agccgttctt cagtgcgacg gcggtctgtg cggcgtcgac
     4801 cggcagcacc ttgaccttgc tggagacgat gtcgctggcc gtcgagaacg gcgtgccgcc
     4861 gtctttcaac gtcaggagct tcgcggactg cagcacgagc agcccccggg cctcgttgat
     4921 cgcgtcgttc ggcagtgcga cggtgccacc cttcggcagg gcggagaccg acgtgtattt
     4981 cgtggaatac agcggcagcg ggtagacggc cgtcgcgccg atcggctgca gatcgtcgtg
     5041 ggcggagacg ttgtagtcgg caaggaactc gatgtgctgg aattcgttca ggtcgagctg
     5101 gccttggctg agtgcgggat tgggctgtga gtaatcggtg aagttgacga tcttgacctt
     5161 gacgtcgagc tctttggccg cgagacggga gaacgtcttc cagtacggtt gcgcggcgtc
     5221 ggtgacgcca atggagacgg tcttcgcgct cgccttcgcc gtctcgcccg tggtgcggtt
     5281 gccgacgacg atgcccgtga cggtgatggc ggcggccacg acgaccgcga cgatcaccca
     5341 gccgagggtc ttacggcccc gcttcttcgt cgccttctcc ggcagcttcg gcgcctggcg
     5401 gggcgggttt tgaccgttct gcgaatcgga catgctcttc ttcctccgtg gacctcatgg
     5461 tgtgtgggcg tctgacgcac gcctcgccga gtgccgtggc tcccgccggc cggagcccgt
     5521 cgatgcggcg cttcgacgcc gaaccggggg catcgccgcg cacgcgcccg tctatcatcc
     5581 ctgcggcatc gggcgccggc aaagtcgtgt gacgcatcgt gacgcgcctg gcggcagaaa
     5641 cgctcaggcg tgggcgatgc caggcccgcc ggcagagccg acgccctccg gcttacctct
     5701 ggggtaggcg agcgcgacgc cgaatgccgc gacgccgacg aggccggcca cgacgaacgc
     5761 gagccgaatg ccggaggcgc cgggcaggcc ggaggagtcg acgccgccca gagccgcgac
     5821 ggcgacgaac acggcgctgc cgagggcgcc ggcgacctgt tgcagggtgg tgaggagcgc
     5881 gctgccgtgc gaatagaggt cgtcggtcaa ggcgccgagg gaatcggcca tgagcggcgt
     5941 catcatgacc ccgagcgacg acatcatgag catgtcgatg ccgatcaccg ccgcgagcgg
     6001 ggccgcggag ccgaggaggg ccaagaggaa aagggcgacc gacattccgg cggcgcccgg
     6061 tatcacgagc gccttcgcac cgacccggtc gtagagcgcg ccgacggggc gggagacggc
     6121 ggccatcatg acgccacccg gcaagagcgc gaggccggcg acgaagacgc cgtggccaag
     6181 cacgtgctgc aggtagatgg gcagaaggac cgcgccggcg ccgagaagcg ccatgaagac
     6241 cagcatcgag aggatgaggg cgatgagata acgcggcacg gccagtgtgc gcaggtcgag
     6301 gagtggccgg tcacggcgtt gcaggcgggc ctggcgccag gcgaagccgg ccaaggcgag
     6361 ggcgccgacc ggcagggcaa gccacggggg caccgcctgg ccctcctgcc caagcgtgga
     6421 aaggccgtag acgagaccgg cgaagccgag cgcggagagc acgacagaga cggcgtcgag
     6481 cggcacggcg cgtgtcgtgc ccggcacgtg cagccagaga agggcgaggg cgagcatcac
     6541 cgccgcgagg atgagaacca gccagaacat ccaccgccag ccgaggcccg ccagaatcgc
     6601 gccgccgacc gtcggcccga cggccggcgc caccccgatg acgatcgaga tcgtgcccat
     6661 gacggtgccg cggcggccgg gcgcgacgag gcgcatgacg gtggtcatga cgagcgggat
     6721 catcaccgcc gtgccacagg cctgcaccat gcggccgatg agcagggctg gaaagctccc
     6781 ggcgagcc
//
LOCUS       DASTNL010000087        14150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1178333, whole genome shotgun sequence.
ACCESSION   DASTNL010000087 DASTNL010000000
VERSION     DASTNL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 14150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14150
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178333"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /locus_tag="VFQ96_02855"
     CDS             <1..2623
                     /locus_tag="VFQ96_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU0256759.1"
                     /translation="AWLATAHGVGALARTLGPEMPAEERRDGLPFALVLLAVAGAVVE
                     WFRPGREVASWLDAYSFGGLFGLAAYVLPVVMFLFAVWLFRHPAPASDNTRIGIGLAL
                     LVVAGSGLCHLYGGRPSPGEGMPALATGGGVVGWLAAAPLALLVTPLVTTVVMALVMA
                     LAALIVTKTPPTSVVRRLRQLYDYLFGVERADGAEGGDGAPGEEGLAPGEAEDRLPWW
                     RRHAGRGEEEPAYDSPVLSDDEPGGVAAQTGAGAEAGHGEEPAVIEGSPFGADAGPVL
                     TPEAAAGRTPSHSESPTSVIPRGASGADGDLPDGADSADASAQGAGERPYRLPDIRLL
                     VPGAPTKTRSEANDRVVAAITDVLSQFKVRARVTGYSRGPTVTQYEVELGTGVKVERV
                     TALTKNLAYAVASNEVRILSPIPGKSAIGIEIPNSDREIVALGDVLRSPIAAKSAHPM
                     TIGLGKDVGGGYVLAKLTKMPHLLVAGATGSGKSSFVNSMITSILMRATPAEVRLVLV
                     DPKRVELSVYAGVPHLITPIITSPKKAAEALEWVVKEMDMRYDDLASYGFRHIDDFNA
                     AVRAGEIQPPEGSKRALKPYPYLLVVVDELADLMMVAPHDVEDSIVRITQLARASGIH
                     LVLATQRPSVDVVTGLIKANIPSRLAFSVASVTDSRVILDQSGADKLIGQGDALFLPM
                     GASKPIRVQGAWVSEAEITDVVAHVTGQARPEYRKDVQAAAAKKQVDADIGDDLEVLL
                     AAAELIVSTQFGSTSMLQRKLRVGFAKAGRLMDLLESRDVVGPSEGSKARTVLVKPEQ
                     LHDVLAMLRGEGGGAADARQASQDSGGPRGFAADPSEQGAGPAVSHAGDPVDEQYAGY
                     PVVESSDEDAWQLTGRD"
     gene            2625..3245
                     /gene="pgsA"
                     /locus_tag="VFQ96_02860"
     CDS             2625..3245
                     /gene="pgsA"
                     /locus_tag="VFQ96_02860"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU0256760.1"
                     /translation="MRQAGVAGVTAPTAGPGNWNLPNAITIVRILFAPAFLWLLLADQ
                     HADGTLRWVATALFVIGIATDGVDGHIARSRGLVTDLGKLLDPIADKILTGAALVGLG
                     LLAELPWWVVAVILVREWGITVWRFAVIKHGVVAASRGGKLKTVFQSVAIGCALAPLW
                     TLTGGWVFWLDGILMTIAVLLTVVTGIDYLVSYARARRAGGAGRRS"
     gene            3242..3793
                     /locus_tag="VFQ96_02865"
     CDS             3242..3793
                     /locus_tag="VFQ96_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEU0256761.1"
                     /translation="MSGVAGRHDGREEDPAQTAVGADLTARVLAALAAARQTVAVAES
                     LTAGAVLAELTRPAGASASVLGGFVVYATELKHTLLGVDEGLLEREGPVHPEVARQLA
                     EGVRHTLVVAGRHPDFALATTGVAGPASQGGRPPGTVYLGIARAGHVRAVELSLTGDR
                     GEIRERTVACAVRELAREVGLPL"
     gene            3982..4293
                     /locus_tag="VFQ96_02870"
     CDS             3982..4293
                     /locus_tag="VFQ96_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0256762.1"
                     /translation="MILVRQEIGDVLRDCRLRKGHTLRQVASRASVALGYLSEVERGQ
                     KEASSEILASVADALDTPISTIMREVGDRIAVLEGVDPMIPDTVPEEFVSALDVDLVA
                     H"
     gene            4309..4533
                     /locus_tag="VFQ96_02875"
     CDS             4309..4533
                     /locus_tag="VFQ96_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HEU0256763.1"
                     /translation="MRLSEFRRAVDDEYGSRYGRVVTHDLALASLGGRTADEALGEGE
                     EAMAVWLALCEATEVPPDRRYGVGRPLPRR"
     gene            4710..5777
                     /gene="recA"
                     /locus_tag="VFQ96_02880"
     CDS             4710..5777
                     /gene="recA"
                     /locus_tag="VFQ96_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU0256764.1"
                     /translation="MPSSADREKALETALAQIDRQFGKGSVMRLGSDERAPVEVIPTG
                     SVALDVALGIGGLPRGRVVEIYGPESSGKTTLTLHAIANAQQAGGIAAFIDAEHALDP
                     EYAAKLGVDIDALLVSQPDTGEQALEIADMLVRSGSLDIIVVDSVAALVPRAEIEGEM
                     GDSHVGLQARLMSQALRKLTGGLSQTGTTMIFINQLREKIGVFFGSPETTSGGKALKF
                     YASVRLDIRRIETLKDGVEAVGNRTRVKVVKNKMAPPFKKAEFDILYGTGISREGSLL
                     DFGVDHGIVKKSGAWYTYEGDQLGQGKENSRKFLLANPDIAAEIEHKIKVKLGLIAAP
                     EEASVDNVDSLAEKLAARKGA"
     gene            5774..6490
                     /locus_tag="VFQ96_02885"
     CDS             5774..6490
                     /locus_tag="VFQ96_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEU0256765.1"
                     /translation="MTGATVTEEGHLAPVVYLHASARRAARQAGLVRAGQAGRSAPIG
                     QAGPNDQASEKLPGGRTAPGTGDDAEAARLALQQLARHDASRRELADMLRRRGIGGQV
                     AEQEVARLAAEGLADDGRLARNLVETRFARKGLGLAGMRRELGRRGIADDDIDAALAG
                     LSDEDDRGRALEVARARATHLGQADRRSARRRLGAYLARRGYSGLVVESVVREVLSDS
                     DRDDATFGGEGGGAGAPEPA"
     gene            6487..7419
                     /gene="miaA"
                     /locus_tag="VFQ96_02890"
     CDS             6487..7419
                     /gene="miaA"
                     /locus_tag="VFQ96_02890"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU0256766.1"
                     /translation="MSRPAHFVAIVGATGTGKSSLSLSLAEHIAACGGRAEIVNADAM
                     QLYRGMDIGTAKVPQAERRGVAHHLLDVLAVTDEASVARYQREARAAVTEILLRGHVA
                     ILVGGSGLYVSAVIHDFTFPPHDVALRRSLEEEAARHGLAPLVRRLRAHFPEAAQRVD
                     LENPRRVVRALEAAQLGEAGQIGRLPDPPRPWRDVTVVGLHAERGNLVSALDERVRRM
                     WRDGLVREVEGLLAAGLARATTAGRAIGYAQALAQLRGESAADEAIAETQRRTRRYAR
                     RQEGWFRRYAEARWITVVPGEAPPTRAAAAHAGW"
     gene            7452..8333
                     /gene="dapF"
                     /locus_tag="VFQ96_02895"
     CDS             7452..8333
                     /gene="dapF"
                     /locus_tag="VFQ96_02895"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU0256767.1"
                     /translation="MARTLEFTKGQGTGNDFVLFTDPDDEIRLTDRQVAEICDRRFGV
                     GADGMIRAVRTQSLAEGEPLVEADPGCEWFMDYRNADGAAAEMCGNGIRVYARYLVEN
                     KLASLEPGRTLTIGTRAGLVDVQTNRTGFQADLGTWRLAGGEPLVRARGLPVARPGLG
                     IDVGNPHVVVALADDDELDGMDLGYVPKLEPQPQAGANVEFVVPGEPLVQDGIGRIRM
                     RVHERGSGETLSCGTGAVAAALATRHWAGKGAPDMWRVRVPGGTLGVRMFGAEDGEHV
                     SLSGPAELTFTGRLHLA"
     gene            complement(8308..8952)
                     /locus_tag="VFQ96_02900"
     CDS             complement(8308..8952)
                     /locus_tag="VFQ96_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0256768.1"
                     /translation="MVSEHYFTRTPAGDPTRRPLTALLAGKAVHVHSAGGVFSPRRVD
                     TGTSLLLECAPPPPARGELLDLGCGWGPIALTLALLSPEATVWAVDVNDRALELVREN
                     SSALGLPGVRPARPEEVPAQIRFQTIWSNPPIRVGKKALHDMLAAWLPRLAADGEAWL
                     VVQKNLGADSLQGWLNEQPGLTAERAGSRKGYRIVRVGRSPGQAPALRPGAAGR"
     gene            9036..10562
                     /gene="hflX"
                     /locus_tag="VFQ96_02905"
     CDS             9036..10562
                     /gene="hflX"
                     /locus_tag="VFQ96_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU0256769.1"
                     /translation="MTGIQGRTSATSHPAQTHGGVSSEPLDAEASALMRPGTAGGDTD
                     GDQFDRQARHALRRVAGLSTELADVTEVEYRRVRLERVVVVGVYQNDQAAAEESLREL
                     AALCETAGSAVLDGLLQRRPHPDPATYLGRGKAEELRGIVLALEADTVVADTELPPSQ
                     RRALEDVVGVKVIDRTAVILDIFGQHATTREGKAQVELAQLQYLLPRLRGWGESMSRQ
                     AGGQVGAGSGMGSRGPGETKIELDRRRIHVRMARLRDEIAKFGPAREVKRSRRRARAV
                     PSVAIVGYTNAGKSSLLNRITRAGLLVENALFATLDATVRRTRTADGRVFTLTDTVGF
                     VRDLPEELVEVFRSSLEAVEECDLVVHVVDAAHPDPAGQIATVRGVLGEVGVHDMPEI
                     VAFNKADLVGADERLVLRGVEPGALFVSARTGEGVGELFDAIAARLPEPPVRVDALIP
                     YGRGDLVSLLYERGRVLDMEYRPEGTRIAARVFPADENAFRPFLTAAVAPGAAGDVVV
                     "
     gene            10731..11729
                     /locus_tag="VFQ96_02910"
     CDS             10731..11729
                     /locus_tag="VFQ96_02910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU0256770.1"
                     /translation="MTARVDVGFSFELYPPRSAQVAARMPQIVRALAETAPRFISVTY
                     AASGASRDASRAVLAEVRASGARPMAHLTCVRSSFDAAHRLVRRFLDDGITDFLAVRG
                     DPPADGVGDGLGEIRSAAELVQLIHRVHAERVPYDEVPVPGLPGAARVGRRSDEVHIA
                     VAAFPGGHPASRSLAQDVDTLLAKEAAGATLALTQLFFHADDYFRLVERARAGGVRMP
                     IVPGIMPVTSPARLARMLELSGQPEPYELAIALEVEPSVEGRRAIGIGHAARLARTLI
                     EGGAPGIHLYTFNREHTALAVLREAGLVGDAHRPAARIGPQRIPNLVPNLPQEGES"
     gene            11810..14134
                     /gene="metE"
                     /locus_tag="VFQ96_02915"
     CDS             11810..14134
                     /gene="metE"
                     /locus_tag="VFQ96_02915"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0256771.1"
                     /translation="MGYPRIGRGRELKRALEAYWSGKSDRATLETTARRLRAESRSRL
                     STLGLDPRADAIPEDFSYYDQVLDAAVTVGAVPTRFSDLRGPDGHVDLDGYFTLARGD
                     GKRAPLEMTKWFNSNYHYLVPEIGPETRFRLDARRLLAHVAEAHEQGIAVRPVIVGPV
                     TLLALSKPEESHAGFRPLKVLDAVVAVYGELLERLAAAGVRWVQFDEPALVSEGLEPP
                     AGAGEPRLADLAAGAYRTLQAIVQRPAIHVAAPYGDLGDGFQALALSGVEAIGLDLVH
                     GDVPACSDLDLSGLTLVGGVVDGHNVWRTDLGAALAGLRQLRRLGASVSVSTSTSLFH
                     VPYDVGVERALPGRLQSWLAFADQKVEEVVTLGRALTDGEDCVRAALDASAALLAERA
                     AAPGVRDAGVRERAAALDEAAYRRGDYETRRRAQARRLGLPLLPTTTIGSFPQTADIR
                     RSRAAFARGELGRDDYVARMRKEIRDVIERQERLGLDVLVHGEAERNDMVQYFAENLA
                     GFAVTRDGWVQSYGSRCTRPSILWGDVRRTHPITVEWSSYAQSLTDKPVKGMLTGPVT
                     ILAWSFVRDDQPLASTATQVALALRDEIADLEKAGLAVIQVDEPALRELLPLKRADQP
                     DYLAWSVDAFRLATAGAADDTQVHTHLCYSEFGVVMDAIRRLDADVTSIEAARSRMEV
                     VTDIVAAGFRHGIGPGVYDIHSPRVPGEEEIQGLLRRAVDTIDARQVWVNPDCGLKTR
                     TYAEVTPSLEHLVEAARTVRAELAGTGAVATAGA"
BASE COUNT         2057 a         4955 c         5039 g         2099 t
ORIGIN      
        1 ggcctggctt gcgaccgcgc acggcgtggg cgccctggca cgcaccctcg gccccgagat
       61 gccggcggag gaacgccgtg acgggctgcc cttcgccctc gtcctcctcg cggtcgccgg
      121 ggcggtcgtc gaatggttcc ggccgggccg cgaggttgcc tcgtggctcg acgcctacag
      181 cttcggcggg ctcttcggcc tggccgccta cgtcttgccc gtcgtcatgt tcctctttgc
      241 cgtgtggctg ttccgccacc cggcgccggc gtcggacaac acgagaatcg gcatcggcct
      301 ggccctgctc gtcgtggcgg gctcgggcct gtgccacctg tatggcggcc gtccgtcgcc
      361 gggcgagggc atgcccgccc tggcgacggg cggcggcgtc gtcggctggt tggcggccgc
      421 gcccctggcg ctcctcgtga cgccgctcgt gacgaccgtc gtgatggccc tcgtcatggc
      481 actcgcggcc ctgatcgtca cgaagactcc gccgaccagc gtcgtgcgca ggctgaggca
      541 gctttacgac tacctcttcg gcgtcgagcg tgccgacggc gccgagggcg gtgacggggc
      601 gccgggcgag gagggcctcg cgccggggga ggcggaggat cgcctgccgt ggtggcggcg
      661 tcatgccggg cggggcgagg aagagccggc ctacgactcg ccggtgcttt ccgacgacga
      721 gccggggggc gtcgccgccc agaccggcgc cggcgccgag gctggccacg gcgaggagcc
      781 ggcagtcatc gaaggctcgc ccttcggggc cgacgccggc ccggtgctga ctcccgaggc
      841 ggctgcgggc cggacgcctt cgcattccga gtcgccgacg agcgtcatac cgcgcggagc
      901 ctccggcgcg gacggcgacc tgcccgacgg ggccgactcg gccgacgcga gcgcgcaggg
      961 cgcgggcgag cggccgtacc ggctgcccga catccggctc ctcgtgccgg gtgccccgac
     1021 caagacgcgt tccgaggcca acgatcgcgt cgtcgccgcc atcaccgacg tgctctcgca
     1081 gttcaaggtg cgcgcgcggg tcacgggcta ttcgcgcggc ccgacggtga cgcaatacga
     1141 ggtggagctc ggcaccggcg tcaaggtgga acgggtcacg gcgctcacga agaacctcgc
     1201 ctacgcggtc gccagcaacg aggtgcgcat cctctctccg atccccggca agagcgcgat
     1261 cggcatcgag atccccaact ccgaccgcga gatcgtcgcg ctgggcgacg tgttgcgctc
     1321 cccgatagct gcgaagagcg cccaccccat gacgatcggg ctcggcaagg atgtcggcgg
     1381 tggctacgtt ctggcgaagc tgacgaagat gccgcatctg ctcgtcgccg gggccaccgg
     1441 atccggcaag tcgagcttcg tgaactccat gatcacgagc atcctcatgc gcgcgacgcc
     1501 ggccgaggtg cgcctcgtgc tggtcgatcc gaagcgggtg gagctctccg tctacgcggg
     1561 cgtgccgcac ctcatcacgc ccatcatcac gagcccgaag aaggccgccg aggcgctcga
     1621 atgggtcgtc aaagagatgg atatgcgcta cgacgacctg gccagctacg ggttccgcca
     1681 tatcgacgac ttcaacgccg cggtgcgcgc gggtgagatc cagcctcctg agggcagcaa
     1741 gcgggccctc aagccctatc cctaccttct tgtcgtcgtc gacgagctgg cggacctcat
     1801 gatggtcgcc ccgcacgacg tggaggattc gatcgtgcgc ataactcagc tggcccgcgc
     1861 cagcggcatt catctggtgc tcgcgaccca acggccgagc gtcgacgtcg tcaccggcct
     1921 catcaaggcg aacatcccct cccggctcgc gttctccgtg gccagcgtca ccgactcccg
     1981 cgtgatcctc gaccagtccg gcgccgacaa gctcatcggg cagggcgacg cgctcttctt
     2041 gccgatgggt gcctcgaagc cgatccgggt acagggggcg tgggtctccg aggcggagat
     2101 caccgacgtc gtggcgcatg tcaccggcca agcgcggccc gagtatcgca aagacgtgca
     2161 ggcggcggcc gccaagaaac aggtggacgc cgacatcggc gatgacctcg aggtgcttct
     2221 cgccgccgcc gagctcatcg tctcgacgca gttcggctcg acatccatgc tgcaacgcaa
     2281 gcttcgcgtc ggcttcgcga aggcggggcg cctcatggac ctgttggaat ctcgcgacgt
     2341 cgtcgggccc tccgaaggct ccaaggcgcg taccgtcctc gtgaaacccg agcaactgca
     2401 cgacgtgctg gcgatgctgc gcggcgaggg aggcggcgcg gccgacgcgc ggcaggcttc
     2461 acaggattcc ggcgggccgc ggggcttcgc ggcagacccc tcggagcagg gtgccggccc
     2521 ggccgtgagc catgccggcg acccggtcga cgagcagtat gcggggtatc ctgtggtcga
     2581 atcctcggac gaggatgcgt ggcaactgac cggcagggac tgatatgcga caggctggcg
     2641 tcgccggggt gacggcgccg acggcgggcc ccggaaactg gaacctgccg aacgcgatca
     2701 cgatcgtgcg catccttttc gcccccgcgt tcctgtggct gctcttggcc gaccagcacg
     2761 ccgacggcac gctgcgctgg gtcgcgacgg cgctcttcgt gatcggcatc gccaccgacg
     2821 gcgtcgacgg gcacatcgcc cgcagccgcg gcctcgtcac cgacctcggc aagcttctcg
     2881 acccgatcgc cgacaagatc ctgaccgggg cggcccttgt cgggctgggg ctcctcgccg
     2941 aactgccctg gtgggtcgtc gcggtgatcc tggtgcgtga atggggcatc accgtgtggc
     3001 gtttcgcggt gatcaagcac ggcgtcgtcg cggcctcgcg gggcggcaag ctgaagacgg
     3061 tcttccagtc ggtcgcgatc ggctgcgccc tggcgccgct ctggaccttg accggcggat
     3121 gggtcttctg gctcgacggc atcctcatga cgatcgcggt gcttttgacg gtcgtcaccg
     3181 gcatcgacta tctcgtctct tacgcccggg cccgccgggc gggcggtgcg gggcgcaggt
     3241 cgtgagcggg gtcgcgggtc ggcacgacgg ccgggaggaa gaccctgcgc agacggccgt
     3301 gggcgcagac ctgacggcgc gcgtcctcgc ggccttggcc gcggcccggc agacggtggc
     3361 cgtggcggag tcgctgacgg caggcgccgt gctcgccgaa ctcactcggc cggcgggcgc
     3421 ctccgcgtcg gtgctcggcg gcttcgtcgt ctatgcgacc gagctgaagc acaccctcct
     3481 cggagtcgac gagggccttc tcgaacggga ggggcctgtg cacccggagg tcgcccgcca
     3541 gcttgccgag ggggttcgcc atacgctcgt ggtcgccggc cgtcatccgg atttcgcatt
     3601 ggcgacgacc ggggtcgccg gcccggcgag ccaagggggc cggccgccgg gcactgtcta
     3661 cctcggcatc gcgagggccg ggcacgtgcg tgcggtcgag ctctccctga ccggtgatcg
     3721 cggcgagatc cgggaacgca cggtggcgtg cgccgtccgg gagctggcgc gggaggtggg
     3781 cctgcccctg tgaggcacga tgcccgggaa taccgtggtg cgtcacgggc gttggctctg
     3841 taacgaaggc agaacgatgg caaccataca cgcgcgatcc agccggcctg ggtaggctgt
     3901 cattcgatga cgcagtgtag tgttgggcac ccgcacgggt tcccgtatca tccggcagac
     3961 cagcagaggg aggcgcctcc gatgattctc gtccgtcaag agatcggtga tgtgctgaga
     4021 gactgccgtc tccgcaaggg gcacaccctt cgacaggtcg cgagccgtgc cagcgtcgcg
     4081 ctcggctact tgagcgaggt cgagcgaggc cagaaagagg cgtcgagcga aatcctcgcc
     4141 tcggtggctg acgcgctcga caccccgatc tcgaccatca tgcgtgaggt cggcgaccgc
     4201 atcgccgtgc tcgaaggggt cgacccgatg attcccgaca ccgtgcccga ggagttcgtc
     4261 tcggcgctcg acgtggatct cgtcgcgcac tgagcgcggc ccgtcggcat gcgcctgagc
     4321 gagttccgcc gcgcggtgga tgacgagtac ggcagccggt acggccgggt ggtgacgcac
     4381 gacctggcct tggcgagctt gggcggccgc accgccgacg aggcactggg tgagggcgaa
     4441 gaggcgatgg ccgtgtggct cgcgttgtgc gaggcgaccg aggttccgcc ggatcgccga
     4501 tatggtgtgg gccggcctct gccacgccga tagggctatc gaagatgtgt tcgatttttg
     4561 ctatgctctg cacagcgagc tttcttcggg gggttcacca ccgggcggcg tgattccgcg
     4621 gcgatgtcgg cggcaacgca tacagtcgtc atcgccgggg gtgccatgca ggggtgcggt
     4681 gcccggaccg agtgaagaaa agagacacga tgccatcgtc agcagaccgt gagaaggcgc
     4741 tggagaccgc gctggcccag atcgatcgcc aattcggcaa ggggtccgtg atgcggctgg
     4801 gcagcgacga gcgtgcgccc gtcgaggtga tcccgaccgg gtcggtggca ctcgatgtcg
     4861 ccctcggcat cgggggcctg cctcgcggcc gggtggtcga gatatacggc cccgagtctt
     4921 ccggcaagac cacgctcacc ttgcacgcca tcgcgaacgc gcagcaggcg ggcggcatcg
     4981 ccgccttcat cgacgcagaa cacgccctcg acccggaata cgcggcgaag ctgggcgtcg
     5041 acatcgacgc cctcctcgtc tcacaacccg acacaggaga acaggcgctc gagatcgccg
     5101 acatgctcgt gcgcagcggg tcgctcgaca tcatcgtggt cgactcggtc gccgccctcg
     5161 tgccgcgggc cgagatcgag ggagaaatgg gcgactcgca tgtcggcctt caggcgcggc
     5221 tcatgtcgca ggcgttgcgc aagctgaccg gcgggctcag ccagaccggc acgaccatga
     5281 tcttcatcaa ccagttgcgt gagaagatcg gggtcttctt cggcagcccc gagacgacct
     5341 ccggcggcaa ggcgctcaag ttctacgcgt cggtacgcct cgacatccgc cgcatcgaga
     5401 cgttgaagga cggcgtcgag gcggtcggca accggacccg ggtcaaggtg gtcaagaaca
     5461 agatggcgcc gccattcaag aaggcggagt tcgacatcct ctacggcacc ggcatctccc
     5521 gcgaggggag cctgctcgac ttcggcgtcg accacggcat cgtgaagaag tccggcgcct
     5581 ggtacaccta cgagggcgac cagctcggcc agggcaaaga gaactcgcgc aaattccttc
     5641 tcgcgaaccc cgacatcgcg gcggagatcg agcacaagat caaggtcaag cttggcctca
     5701 tcgctgcacc agaagaggcc tccgtcgaca acgtcgactc cctcgccgag aagctcgcgg
     5761 cccgcaaggg cgcgtgacag gcgccactgt gacggaagaa ggccacctgg cgcccgtcgt
     5821 ctacctacac gcgtcggctc gacgggctgc gcggcaggcc ggtctcgttc gggccggcca
     5881 ggccggccgg agtgccccga tcggccaggc cggcccgaac gaccaggcat cggagaagct
     5941 gccggggggg cggacggcgc cgggcacggg tgacgacgcc gaggcggcgc ggctcgccct
     6001 gcagcagttg gccaggcacg acgcctcccg ccgggagctg gccgacatgc tgcgtcggcg
     6061 gggcatcggg ggccaagtcg ccgagcaaga ggtcgcaagg ctcgctgccg agggcctcgc
     6121 cgacgacggg cgcctcgccc gcaacctggt cgagacccgc ttcgctcgca aaggcctggg
     6181 gttggccggc atgcgccgcg aactgggccg gcgcggcatc gccgacgacg acatcgacgc
     6241 cgcgttggcg ggcctcagcg acgaagacga ccgcggcagg gccctcgagg tggcgcgggc
     6301 ccgcgccacg catctggggc aggccgatcg gcgctcggcc cgccgtcggc tgggggctta
     6361 cctggccagg cgcgggtact cgggtctcgt cgtcgaatcc gtcgttcgtg aggtgctttc
     6421 cgactccgat cgcgacgacg cgacgttcgg cggcgagggt ggtggcgccg gcgcgccgga
     6481 acccgcatga gtcggcccgc gcacttcgtc gcgatcgtcg gtgcgaccgg gacgggcaaa
     6541 tccagcctgt cgctctctct cgccgagcac atcgcggcgt gcggcggccg cgccgagatc
     6601 gtcaacgccg atgcgatgca gctctaccgc ggcatggata tcggcacggc gaaggtgccg
     6661 caagccgagc ggcggggcgt tgcccaccat cttctcgacg ttctcgccgt caccgacgag
     6721 gcttcggtcg cccgctatca acgtgaggcc cgcgccgcgg tgaccgagat cctcctgcgc
     6781 ggtcacgtcg cgatcctcgt cggcggttcg ggcctttatg tctccgccgt gatccacgac
     6841 ttcacgttcc cgccgcacga cgtggccctg cgccgaagtc tcgaggagga ggctgcccgg
     6901 catggcctgg cacccctcgt ccggcggctg cgggcgcact ttcccgaggc ggcgcagcga
     6961 gtcgacctgg agaacccacg gcgcgtcgtt cgcgccctcg aggcggcgca gctgggggag
     7021 gccgggcaga tcggccggct gcccgacccg cccaggccgt ggcgtgacgt cacggtggtc
     7081 gggttgcacg ccgaacgcgg caaccttgtg tcggcccttg acgagcgggt gcgccgcatg
     7141 tggcgcgacg ggctcgtgcg ggaggtcgag ggtctcctcg ccgccgggct cgcccgggcc
     7201 acgaccgcgg ggcgcgccat cgggtacgcg caggcgctcg cccagctgcg gggcgagagc
     7261 gcggccgacg aggcgatcgc cgagacgcag cggcgcaccc ggcgttacgc acggcgtcaa
     7321 gaaggctggt ttcgccggta tgccgaggcg cggtggatca ccgtcgtgcc gggcgaggct
     7381 ccacctactc gggcggcggc cgcgcacgcc gggtggtgag gcggggtctc gccggccctt
     7441 acactggtcg ggtggcgagg accctggaat tcacgaaagg ccaaggaacc ggcaacgact
     7501 tcgtgctctt caccgatccg gatgacgaga tccgtctgac cgaccggcag gtcgccgaga
     7561 tctgtgaccg ccggttcggg gtcggcgccg acggcatgat ccgcgcggtg cgcacccaga
     7621 gcctcgccga gggcgagccg ctcgtcgagg cagaccccgg gtgcgaatgg ttcatggact
     7681 atcggaacgc cgacggcgcg gcggccgaga tgtgcggcaa cggcatccgc gtctatgccc
     7741 gatacctcgt cgagaacaag ctggcaagcc tggagccggg ccgcaccttg accatcggca
     7801 cccgagccgg ccttgtcgac gtgcagacca atcgcaccgg gttccaggca gacctcggca
     7861 cctggcgcct ggccggcggc gaaccgctgg tccgcgcgcg ggggctcccc gtcgcccggc
     7921 ccggcctcgg gattgacgtc ggcaatcccc atgtcgtcgt cgcgctggcc gacgacgacg
     7981 agctcgacgg catggatctc gggtacgttc cgaagctgga accgcagccg caggcggggg
     8041 cgaatgtcga attcgtcgtg cccggggagc cgctcgtgca agacggcatc ggccgcatcc
     8101 gcatgcgggt gcacgaacga ggcagcggcg agacgttgtc gtgcggcacg ggcgccgtcg
     8161 ccgcggcact cgcgacgaga cactgggccg gcaagggggc gcccgacatg tggcgggtgc
     8221 gtgtgcccgg cggcacgttg ggcgtgcgga tgttcggggc cgaagacggc gagcatgtct
     8281 cgttgtcggg cccggccgag ttgacgttca ccggccggct gcacctggcc tgagggcggg
     8341 cgcctgccct ggggaacgcc cgacccgcac gatgcggtaa cccttgcgag agccggcccg
     8401 ttctgccgtg aggccgggct gctcgttcag ccagccctgc aacgagtcgg caccgaggtt
     8461 cttctgcacc accagccatg cctcgccgtc ggccgccagc cgtggcagcc acgccgccag
     8521 catgtcgtgc aacgcctttt tacccacccg gatcggcggg ttcgaccaga tcgtctggaa
     8581 ccggatctgc gccgggacct cctccggccg agcaggcctg acgcctggca gacccaaggc
     8641 gctggagttc tcgcgcacga gctccagtgc ccggtcgttc acgtcgaccg cccacaccgt
     8701 cgcctccggc gagaggagcg cgagcgtgag cgcgataggc ccccatccgc agccaaggtc
     8761 gagaagttcg ccccgggccg gcggcggtgg ggcgcattcc aagagcagac tcgtgccggt
     8821 gtccacccgg cgagggctga acacgccgcc cgcgctgtgc acgtgcaccg ccttgcctgc
     8881 caggagcgcc gtgagggggc gtctcgtggg gtcgccggcc ggcgtgcggg tgaagtagtg
     8941 ctcggatacc acgagaaaac cgtatcgaac gctcgttccc gcggcgggaa cgggctgccc
     9001 gccccgttcg gcctcgtcgc ataggatggt cccagatgac gggtatccaa ggccgcacgt
     9061 cggccacatc gcaccccgcc cagacgcacg gcggggtgtc ctccgagccg cttgacgcag
     9121 aggcctctgc gctcatgcgc cccggcacgg ccggcggcga caccgacggt gaccagttcg
     9181 accggcaggc gaggcatgcg ctgcgtcgcg tcgccgggct gtccaccgag ctggccgatg
     9241 tcaccgaggt cgaataccgg cgggtgcggc tggagcgggt cgtcgtggtc ggcgtctacc
     9301 agaacgacca ggccgccgcc gaggagtcgt tgcgcgagtt ggcggccctc tgcgagaccg
     9361 ccgggtcggc ggtgctcgac gggctgctgc aacgccgccc gcatcccgac cccgccacct
     9421 acctcggccg aggcaaggcc gaagaactgc gaggcatcgt gctcgccctg gaggccgaca
     9481 cggtcgtcgc cgacacggag ctgccgccca gccagcgccg ggcgctcgaa gatgtcgtag
     9541 gcgtgaaggt catcgaccgc accgccgtga tcctcgacat cttcggccag cacgcgacga
     9601 ctcgggaggg caaggcgcag gtcgagctcg cgcagctgca gtatctgctg ccccggcttc
     9661 ggggctgggg cgagtcgatg tcgcgtcagg ccggcggcca ggtcggcgcc ggatcgggaa
     9721 tgggttcgcg tgggcccggc gagacgaaga tcgagctgga ccggcgtcgt atccacgtgc
     9781 gcatggcccg gctgcgcgac gagatcgcga agttcggccc cgcacgcgag gtcaaacgat
     9841 cccggcgccg ggcgcgggca gtgccttccg tcgcgatcgt cggctacacg aatgccggca
     9901 agtcgagcct cttgaaccgc atcacacggg cgggtctcct cgtcgagaac gcgttgttcg
     9961 ccaccctcga cgcgacggtg cgacgcaccc gtacggccga cggccgggtc ttcaccttga
    10021 ccgacacggt cggcttcgtg cgggaccttc ccgaggagct cgtcgaggtc ttccgctcca
    10081 gcctcgaggc ggtcgaagaa tgcgacctgg tggtgcatgt cgtcgacgcc gcgcaccccg
    10141 acccggcggg gcagatcgcg acggtgcgcg gcgtcttggg cgaggtcggc gtgcacgaca
    10201 tgcctgagat cgtcgccttc aacaaggccg acctggtagg ggccgacgaa cgcctcgtct
    10261 tgcgtggcgt cgagcccggg gcactcttcg tctccgcccg caccggcgaa ggcgtcggcg
    10321 agctcttcga cgcgatcgcc gcgcgactgc cggagccgcc ggtgcgggtc gacgcgctca
    10381 tcccctatgg ccgcggcgac ctggtgtcgc tcttgtacga acgcggccgg gtcctcgaca
    10441 tggagtaccg gcccgagggc acccgcatcg cggctcgcgt ctttccggca gacgagaacg
    10501 cctttcgccc cttcctcacc gcagccgtgg caccgggcgc ggccggtgat gtcgttgtgt
    10561 gacatcttcc gtccggccgc tcggcgcggt tgctactgtt acatcaaccg gtcctagggc
    10621 cggaggaggc agcgccgagc ccggcaattg ccccgcaacg agccgtgagg ctctaccctg
    10681 ccgcagtctt cggcggcagg atatgcgagg agagacggga ggcacggcgt atgacggcac
    10741 gggtcgacgt cgggttctcc ttcgaactgt atccgccgcg ctcggctcag gtggccgcgc
    10801 ggatgccgca gatcgtgcgc gccttggccg agaccgctcc ccgtttcatc tcggtcacgt
    10861 acgcggcctc gggcgcctca cgagacgcct ctcgcgcggt gctggccgag gtcagggcgt
    10921 cgggcgcgcg ccccatggca cacctgacgt gtgtgcgctc gagcttcgac gcggcgcacc
    10981 ggctcgtccg gcggtttctc gacgacggca taaccgactt cctcgccgtg cgcggcgacc
    11041 cgcccgcaga cggcgtcggt gacggcctgg gagagatccg cagcgccgcc gaactcgtgc
    11101 agctgatcca ccgggtgcac gccgaacgcg tgccctacga cgaggtgccc gtgccgggcc
    11161 tgcccggggc ggcccgggtg gggcgccgca gcgacgaggt gcacatcgcc gtcgcggctt
    11221 ttccgggcgg gcaccccgcc tcgcgcagcc tggcccaaga cgtcgacacg ctgctcgcca
    11281 aagaggcggc cggcgcgacc ctcgcgctga cgcagctgtt ctttcacgcc gacgactact
    11341 tccgcctcgt cgaacgtgcc cgcgccggcg gcgtgaggat gccgatcgtc ccggggatca
    11401 tgcccgtgac gagcccggcc cgtctcgccc gcatgctgga actgagcggg cagcccgagc
    11461 cttacgaact cgcgatcgcg ctcgaggtcg agccgagtgt cgagggccgg cgtgccatcg
    11521 gcatcggcca cgccgcccgt ctcgcccgga ccctcatcga gggcggcgcg cccggcatcc
    11581 acctctacac cttcaaccgt gaacacaccg cgctcgcggt gctgcgcgag gctggactgg
    11641 tcggcgatgc ccatcgcccc gccgcccgga tcggcccaca gcgcataccg aacctcgtac
    11701 ccaacctccc acaggaaggc gaatcgtgac cgcccagcaa aaccccgccc aggcccgttc
    11761 ggaaaggccc agcccggccg cccgcgcggc gttcccgaca gccacgatca tgggttaccc
    11821 gcgcatcggt cgggggcgcg agctgaaacg cgccctcgaa gcgtattggt ccggcaagag
    11881 cgaccgggcg acgctcgaga cgaccgcgcg gcgactgcgt gccgaaagcc gctcgcggct
    11941 ttcgacgctc ggcctcgatc ctcgggcaga cgccatcccc gaagacttca gctactacga
    12001 ccaggtgctc gacgccgccg tcaccgtcgg ggccgttccc acccgcttca gcgacctccg
    12061 ggggcccgac gggcatgtcg acctcgacgg ctatttcacc cttgcccgcg gcgacggcaa
    12121 gcgtgcgccg ttggaaatga cgaaatggtt caactccaac tatcactacc tggttccgga
    12181 gatcgggccc gagacccgct tccgcttaga cgcccggcgc ctcctcgccc acgtcgccga
    12241 ggcacacgag cagggcatcg ccgtcaggcc ggtgatcgtc ggcccggtca cgctgttggc
    12301 gctcagcaaa ccggaggagt ctcacgcggg attccggccg ctcaaggtgc tggacgcggt
    12361 cgtcgcggtc tatggcgagc tcctcgagcg cctggcggcc gccggcgtgc gttgggtgca
    12421 gttcgacgag ccggcgctcg tcagcgaagg cttggaaccc ccggccggag ccggcgagcc
    12481 gcggctcgca gacctggcgg cgggcgccta ccgcacgctc caggcgatcg tgcagcgccc
    12541 cgcgattcac gtcgcggccc cctacggcga tctcggtgac ggcttccagg cgctcgcgct
    12601 ctcgggcgtc gaggcgatcg ggctcgacct cgtccacggc gacgtgcccg cgtgctccga
    12661 cctcgacctc tccggcctga ccctcgtcgg cggcgtggtc gacgggcata acgtgtggcg
    12721 caccgatctt ggtgcggcgc tcgccgggct ccggcagctt cggcggctgg gggccagcgt
    12781 gagcgtgtcg acctccacct cgcttttcca cgtgccctat gacgtcggcg tcgagcgggc
    12841 gctgcccggc cggctccagt cgtggctggc cttcgccgac cagaaggtcg aagaggtcgt
    12901 cacgctcggt cgagcgctga ccgacggcga ggattgcgtg cgtgcggccc tcgacgcctc
    12961 cgcggcgttg ttggccgagc gtgccgccgc cccgggcgtg cgcgacgccg gagtgcgcga
    13021 gcgtgcggcc gcgctcgacg aggccgcgta ccggcgtggc gactacgaga cccgcaggcg
    13081 ggcgcaggcg aggcgcctgg gattgcctct gctgccgacg acgaccatcg gatccttccc
    13141 tcagacggct gacatccgcc gctcccgggc ggctttcgcc cgcggcgagc tcgggcggga
    13201 cgactacgtc gcgcggatgc ggaaagagat tcgcgacgtc atcgaacgac aggaacgcct
    13261 gggcctcgac gtcctggtgc acggcgaggc cgagcgcaac gacatggtgc agtacttcgc
    13321 cgagaacctc gccggttttg ccgtcacccg tgacgggtgg gtgcagtcgt atggcagccg
    13381 ctgcactcgg ccgtccattc tttggggcga tgtgcgccgc acgcacccga tcacggtgga
    13441 atggtcgtct tacgcgcaat ctctcaccga caagccggtg aagggcatgc tcaccgggcc
    13501 cgtgacgatt ctcgcgtggt cgttcgtgcg cgacgaccag ccgctggcct cgacggccac
    13561 ccaggtggcc ttggcattgc gcgacgagat cgccgacctg gagaaggccg gccttgccgt
    13621 catccaggtg gacgagcccg ccctgcggga gctgctcccc ttgaaacggg cggaccagcc
    13681 ggactatctc gcctggtccg tcgacgcctt ccggttggcg accgcgggcg cggccgacga
    13741 cacccaggtc cacacccacc tgtgctattc cgagttcggc gtcgtgatgg atgcgatccg
    13801 caggctcgac gccgacgtca ccagcatcga ggcggcccgc agccgcatgg aggtcgtcac
    13861 cgatatcgtg gccgccggtt tccggcacgg catcgggccg ggcgtctacg acatccattc
    13921 gccgcgcgtg cctggcgagg aggagatcca aggtctcttg cgccgcgccg tcgacacgat
    13981 cgatgcacgc caggtctggg tgaaccccga ctgcgggctg aagacgcgta cctacgccga
    14041 ggtgacaccc tcgctggaac acctcgtcga ggcggcacgc acggtgcggg cggagttggc
    14101 cggaacgggg gccgtggcta cagctggcgc atgaccgcga ccacccggcc
//
LOCUS       DASTNL010000088         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_991618, whole genome shotgun sequence.
ACCESSION   DASTNL010000088 DASTNL010000000
VERSION     DASTNL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991618"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..376)
                     /gene="rpsL"
                     /locus_tag="VFQ96_02920"
     CDS             complement(2..376)
                     /gene="rpsL"
                     /locus_tag="VFQ96_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011096633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU0256772.1"
                     /translation="MPTIQQLVRKGRTPKTVSTKAPALKANPQQRGVCTRVYTTTPKK
                     PNSALRKVARVKLSNGTEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIVRGAL
                     DTQAVKGRQQARSRYGAKKEKK"
     gene            complement(661..825)
                     /locus_tag="VFQ96_02925"
     CDS             complement(661..825)
                     /locus_tag="VFQ96_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256773.1"
                     /translation="MNFFLVVIAVVAVILLFAGGLIAVVKWLFWVGVVLALVAIIVWL
                     LRLIRGSRSV"
     gene            997..1530
                     /locus_tag="VFQ96_02930"
     CDS             997..1530
                     /locus_tag="VFQ96_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0256774.1"
                     /translation="MEGDNTTRRVVVGTDGSGHSRQALRWAARIAAAANTQLDVVMAW
                     VPASLAVPTLGADLSGAGPVHPLADRDKEEVARDLAASVEKAFGPDKPKGMRLLVVEG
                     LPDAVLVKASQQALMLVVGSRGRGGLTGLLLGSVSQKVSARASCPVLVVHDGDDPDAP
                     GVSEPNGSAAEPTPDAP"
     gene            complement(1619..2104)
                     /locus_tag="VFQ96_02935"
     CDS             complement(1619..2104)
                     /locus_tag="VFQ96_02935"
                     /inference="COORDINATES: protein motif:HMM:NF040330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6121 family protein"
                     /protein_id="HEU0256775.1"
                     /translation="MGNFRAYAAMVALFAAVLNAALVIGVFGPVSLLTGLEVIPEADA
                     GVLLGPAMVLAATLALFAMLLSRIRIPPDEQRVAVGGALAAGVVAGVVYLVVGGLGYA
                     WARSDPLAGFLFVGRQAALPYVFLAAAIAAVVMFLDTLVLASRVGERGRPRWPWEHDG
                     P"
     gene            complement(2301..>5140)
                     /gene="rpoC"
                     /locus_tag="VFQ96_02940"
     CDS             complement(2301..>5140)
                     /gene="rpoC"
                     /locus_tag="VFQ96_02940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU0256776.1"
                     /translation="RNNRLRRLLDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRPVTG
                     TGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIIVGPTLKMHQCGLPKDMALEL
                     FKPFVIKRLIDLSHAQNIKAAKRMVERARPEVWDVLEEVIRERPVLLNRAPTLHRLGI
                     QAFEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHLPLSVEAQAEARLLMLASNNILK
                     PSDGRPVTVPSQDMIIGLHHLTTLKEGATGEGRAFSSVAEAILAFDQGSLDLNAKVRI
                     RLHDVVFPEGTAPEGYTDGPVIKETTLGRALFNETLPDDYPYVERVTDKSAIAGIVND
                     LAERYPKVKVAEALDNLKDAGFHWGTRSGVTIALSDIVTPPEKGEIIARHEKKAAKVQ
                     AEFDKGLTTEAERRAELVKIWSEATDEVSDAMRKAFPADNTIFRMVSSGARGNWLQVR
                     NIAGIRGLVSDPKGEVIPRPILSSYRDGLSVAEYFIATHGARKGLADTALRTADSGYL
                     TRRLVDVSQDVIIREDDCGTTRGLDKVIAARGADGVLVRDANVENSVFARSLAADAVA
                     ADGTVVAAAGSDVGDVLIDKLVAADIERIKVRSVLTCESAVGVCAACYGRSLATGKLV
                     DIGEAVGIIAAQSIGEPGTQLTLRTFHAGGSASADDITQGLPRVQELFEARTPKGASP
                     IAEAAGRIHIEETDKGRKIVLTPDDGDEVHSYPVLKRSTLLVEDGQHVELGDQFLIGT
                     LDPKEVLRVKGVRAVQEYLVGGVQGVYRSQGVPIHDKHIEVIVRQMLRKVTVVDHGDT
                     DLLPGELVDRSRYNTVNRAALTEGKKTASARQEVMGITKASLATESWLSAASFQETTR
                     VLTQAAMEGKSDPLVGLKENVIIGKLIPAGTGLRRYRDVTVQATAEAREERYPNRLFA
                     GDGQFDPEDLGFVDFDDFTKTENYTPGGTYN"
BASE COUNT          780 a         1777 c         1777 g          806 t
ORIGIN      
        1 attacttctt ctccttcttc gcgccgtacc ggctgcgggc ctgttggcgg cctttaacgg
       61 cctgcgtgtc gagtgcgcca cgcacgatct tgtaccgcac gccggggagg tccttgacgc
      121 gaccgccgcg cacgagcacc atcgagtgct cttgcaggtt gtggccttcg cccgggatgt
      181 aggcggtcac ctcggtgccg ttggagagct tgacgcgggc gaccttgcgt agcgccgagt
      241 tcggcttctt cggcgtggtc gtgtagacgc gggtgcagac cccgcgctgc tgggggttcg
      301 ccttgagggc gggcgccttg gtcgacacgg tcttgggcgt gcgacccttg cgcaccaact
      361 gctgaatggt tggcactgag tgctccttga tctgtactgc acggtgacag gtgttgctgt
      421 gtttgccttg ttccgcccgg cgggcggagc caaactggac cacgacccca cctcgtgccg
      481 ccgtgacgac ggccgaagga cgatgccgtg atatgccgtg ggggccgcgg ctcgcggagc
      541 ctgggcccta catggatcag cgcgaacccg agccttctcg cgcgtgcgca atcggccacg
      601 cgcgtcgagg cgcggcaaag cgcacaccgg gataactata gaggaccacg ggctgggcgg
      661 tcaaacgctc ctgctgcccc ggatgaggcg gaggagccag acgatgatcg cgacgagcgc
      721 gagcacgacg ccgacccaga aaagccattt cacgacggcg atgaggccgc cagcgaagag
      781 caggatcacc gcgaccacgg cgatgaccac caagaagaag ttcacgtcgc cccctttccg
      841 cgagcggccg ccgcggcgcc cgcccgcacc gatccctcta ctgtgcgcgc tcttctcgcc
      901 gggcgctccc ctaaaagaac gatcgggccg gcgtggcgga gccggccccg aagggccatc
      961 ctgggcgcag acgcaggcga gcgagaggag acgatgatgg aaggcgacaa caccacgcgg
     1021 cgggtcgtcg tcggcaccga cgggtcgggg cactcccgtc aggccttgcg gtgggcggca
     1081 cgcatcgcgg cggccgcgaa cacccagctc gacgtggtca tggcctgggt ccccgcctca
     1141 ctcgcggtgc cgacgctcgg cgccgatctc tccggggcgg ggccggtgca tccgctcgcc
     1201 gaccgcgaca aagaagaggt cgcccgcgac ctcgccgcgt cggtggagaa ggccttcggc
     1261 cccgacaagc ccaagggcat gcggctcctc gtcgtcgagg gcctgcccga cgccgtgctc
     1321 gtgaaggcct cgcagcaggc actgatgctc gtcgtcggca gtcgcggccg gggaggactt
     1381 accgggctcc tgctcggctc cgtcagccag aaggtctcgg cccgagcctc gtgcccggtg
     1441 ctcgtcgtgc acgacgggga cgaccccgac gcacccggcg tcagcgaacc gaacggcagt
     1501 gcggcggagc cgacgcccga cgcgccgtga ccctgccgta acccgccgac ccccgggtca
     1561 tgaccgtgcc gtgacgggcc gccgccatgc ccgctctcgg cagcggggcc cgtgtcgctc
     1621 agggcccgtc gtgttcccac ggccagcgtg gccggccgcg ctccccgacg cgggaggcga
     1681 gcaccagcgt gtcgaggaac atcacgaccg cggcgatcgc agcggcgagg aacacgtacg
     1741 gcagcgcggc ctgacggccg acgaagagga accccgcgag ggggtcggag cgcgcccacg
     1801 catagccgag gcctccgacg accaggtaga cgaccccggc gaccacgccc gccgcgaggg
     1861 ccccgcccac cgcgacccgc tgttcgtcag gcgggatgcg gatcctggac aagagcatgg
     1921 cgaagagggc gagcgtggcc gccaggacca tggcggggcc gagcaacacg cccgcatccg
     1981 cctcggggat cacctcgagg cccgtgagaa ggctcaccgg gccgaagacc ccgatgacga
     2041 gcgccgcgtt cagaaccgcg gcgaagagcg ccaccatggc cgcgtaagcg cgaaagttcc
     2101 ccatgcctcc acgctaggcg accgtcggac acccggcatc ccccacgggg cggccggcaa
     2161 ttccgacgac cccggcagga cgggcagccg aagaccgccg cggccacacg gcggccagac
     2221 gcctccgagg cctcgaaacg ccgcacgaga acacgagggc ggggtcggcc atgaagccgc
     2281 cccgccctcg cgcccctcga tcagttgtag gtgccgcccg gcgtgtagtt ctcggtcttg
     2341 gtgaagtcgt cgaagtcgac gaaaccgaga tcttccgggt cgaactgccc gtcgccggcg
     2401 aacagccggt tcgggtagcg ctcttcgcgc gcctccgccg tcgcctgcac ggtgacgtcg
     2461 cggtagcggc gcagcccggt ccccgcgggg atcagcttgc cgatgatgac gttctccttc
     2521 aggccgacga gcgggtcgct cttgccctcc atcgccgcct gggtgaggac ccgggtggtc
     2581 tcctggaacg aggccgcgga aagccacgac tcggtcgcca acgaagcctt ggtgatgccc
     2641 atgacctctt gacgggccga tgccgtcttc ttgccctccg tgagcgccgc acggttcacc
     2701 gtgttgtagc gcgaccggtc gacgagctcg ccgggcaaca gatcggtgtc gccgtggtcg
     2761 accacggtga ccttgcgcag catctggcgc acgatcacct cgatgtgctt gtcgtggatc
     2821 ggcacaccct gcgaacggta tacgccctgc acgccgccca ccaggtattc ctgcacggcc
     2881 cgcacgccct tgacgcgcag cacctccttc gggtcgagcg tgccgatgag gaactggtca
     2941 cccagctcga cgtgttgccc gtcttcgacg agcagcgtgg aacgcttcag taccgggtaa
     3001 ctgtgcacct cgtcgccgtc gtcgggcgtc aagacgatct tgcggccctt gtcggtctcc
     3061 tcgatgtgga tgcgccccgc ggcctcggcg atcggcgagg ctcccttcgg cgtgcgggcc
     3121 tcgaagagct cctgcacgcg aggcagaccc tgcgtgatgt cgtcggcgga ggcggaacct
     3181 ccggcgtgga aggtgcgcag cgtcagctgc gtgccgggct cgccgatcga ctgggcggcg
     3241 atgatgccga ccgcctcgcc gatgtcgacg agcttgccgg tggccaacga acggccgtag
     3301 caggccgcgc acaccccgac cgccgactcg caggtgagca ccgaacgcac cttgatgcgt
     3361 tcgatgtcgg ccgcgaccag cttgtcgatg agcacgtcac ccacatcgct gcccgccgcc
     3421 gcgacgaccg tgccgtctgc cgccaccgca tccgcggcga gagagcgggc gaacaccgag
     3481 ttctcgacgt tggcgtcgcg cacgaggaca ccgtcggcgc cccgggcggc gatgaccttg
     3541 tcgaggcctc gcgtcgtgcc acagtcgtct tcgcggatga tgacgtcttg cgacacgtcg
     3601 accagccgcc tcgtgaggta gccggagtcg gcggtgcgca gcgccgtgtc ggccaggccc
     3661 ttgcgggcac cgtgggtcgc gatgaagtac tcggccacgc tcaggccgtc gcggtaggag
     3721 gagaggatcg ggcgcgggat gacctcgccc ttcgggtcgc tcacgagccc ccggatgccg
     3781 gcgatgttac gcacctgcag ccagtttcct cgggcgcccg acgagaccat gcggaagatg
     3841 gtgttgtcgg cggggaacgc cttgcgcatc gcgtcggaga cctcgtctgt cgcctccgac
     3901 cagatcttca cgagctcggc ccggcgttct gcctcggtcg tgagtccctt gtcgaactcc
     3961 gcctgaacct tggccgcctt cttctcgtgc cgggcgatga tctcgccctt ctccggcggg
     4021 gtgacgatgt cggagagcgc gatcgtgacg cccgaacgcg tgccccaatg gaacccggcg
     4081 tccttcaggt tgtcgagtgc ctcggcgacc ttcaccttgg ggtagcgctc ggccaggtcg
     4141 ttgacgatgc cggcgatcgc gctcttgtcg gtgacgcgtt ccacataggg gtagtcgtcg
     4201 ggcagcgtct cgttgaagag cgcacggccg agcgtggtct ctttgatgac cggcccgtcg
     4261 gtgtagccct cgggtgcagt cccctccggg aacacgacgt cgtggaggcg gatgcgcacc
     4321 ttcgcattca ggtcgaggct gccctggtcg aaggccagga tcgcctcggc gaccgacgag
     4381 aacgcgcgtc cctcgcccgt ggcaccctcc ttgagggtgg tcaggtgatg caggccgatg
     4441 atcatgtctt gcgacggcac cgtgacgggg cggccgtcgg agggcttcag gatgttgttc
     4501 gaggccagca tgagcagcct cgcctcggcc tgggcctcta ccgagagcgg caggtgcacg
     4561 gccatctggt cgccgtcgaa gtctgcgttg aacgcggcgc agacgagcgg gtgcagctgg
     4621 atggccttgc cctcgatgag caccggctcg aacgcctgga tgccgaggcg gtgcagcgtc
     4681 ggcgcgcggt tcaagagcac cgggcgctcc cggatgacct cttcgagcac gtcccacacc
     4741 tcgggccgag cccgttcgac catgcgcttg gccgccttga tgttctgcgc gtgcgacagg
     4801 tcgatcaggc gcttgatgac gaacggcttg aagagctcca acgccatgtc cttcggcagg
     4861 ccgcactggt gcatcttcag ggtcgggccg acgatgatga cggaacggcc ggagtagtcg
     4921 acccgcttgc ccagaaggtt ctgccggaac cggccctgct tgcccttcag catgtcggag
     4981 agcgacttca gcgcacgatt gccggtgccc gtgaccgggc gaccgcggcg gccgttgtcg
     5041 aacagcgcgt cgacagcctc ttgcagcatc cgcttctcgt tgttgacgat gatctcgggg
     5101 gcgcccaggt cgagaaggcg gcgcagccgg ttgttgcggt
//
LOCUS       DASTNL010000089        13981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1364425, whole genome shotgun sequence.
ACCESSION   DASTNL010000089 DASTNL010000000
VERSION     DASTNL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364425"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1655
                     /locus_tag="VFQ96_02945"
     CDS             138..1655
                     /locus_tag="VFQ96_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU0256777.1"
                     /translation="MPDNTDGREAGNDEGQAPYTGALATFEELINESPAPTPDFTTVF
                     RGYDKSEVDQALGAVTARLERQEKESRRALREARQAEDKWEAAQAGLEKMRADLTVAK
                     AQLAQAKQEARALAEEITGADGDGSPTKERFEKILRVAEDQAAELVENAASQARQMLE
                     TTRRDIKVGKDQAKNEAEEILERARNDARQEILRAQQDATQARTQAEREAAAATEKTR
                     QAEQESAAVRSAAERDAAALRAQVTRQTEAARGEAETAIRELQVRTAEFEEAFSRRQA
                     DAQQEFLTLHEQAVAHAERITDDANRQVADALDHARRTTARADDFDRLTRARAEKILA
                     GAKDRAAADLDKARVKAEKILGLISDHALTVLREAEDRARQLRWEQHQTESLTAQIRD
                     LTTPGAAAQPPAPEKPADSAARAVATPPAPGDHTAPRAATPETPGEHAAKTNDAASPA
                     EGSGRRTGPKTSPAGGGEGKVTPTSPAEGVAGMRERAKATRDDSALTVPITRPKA"
     gene            complement(1782..3188)
                     /locus_tag="VFQ96_02950"
     CDS             complement(1782..3188)
                     /locus_tag="VFQ96_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256778.1"
                     /translation="MGDVAEDDWTSLTVVAFGPLAVAAGFGVTVWAAGPPAAAAAFLL
                     GAAVSAARARGAASARRPVAELGIAGVVAAAAALVGGAVALVAAAACLGFLSLVLTGV
                     SLSVLARKFRTRRGDAEAWVAALVGAGVLIGGVPTAVLPVGLGWRVALLVEAAIAIAA
                     AAVLRRHGAVTDRGRLGGPAASRPLVVGSLSNGASGGAGGVLGASFAADLAAALCCVT
                     AVAVLAVLPWSADGRYLLPVGEVAAAVTVAGLAGVGGFAAAGVLPGARPSNGILWCAL
                     AASLAAAAGMLWPPVLLRFYPVLWFTAVAALLGGSFGVAAARLRGMAGRPSLPRTAAR
                     WALVGVAGILAVGTFAYFAAGRSGVAVVAASAIAWLSLTVAGVTWCVVARGRGTSARA
                     SVPAAAAPSQAPDSARVAVPVTTVVMPTAEAASEVPRVSAVEEQHRAPGHVSRVFAWV
                     PRRPATKAKGRAGRPRLR"
     gene            complement(3285..4592)
                     /locus_tag="VFQ96_02955"
     CDS             complement(3285..4592)
                     /locus_tag="VFQ96_02955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0256779.1"
                     /translation="MSEPHDPGRHDRPTASRRLTIVIGADTFPPNVNGAARFAQHLAS
                     GMAARGHDVHIVAPAASRRHGTWSEEYDGQRVTAHRLLSWRWYSPSWLRFALPWRIKE
                     NSARILDEVMPDVVHFQSHIITGRGLSIEAKRRGIRVVGTNHFMPENLFDHAPIPKAL
                     RRLSIRMAWRAAARSFARADVVTSPTPTAAHFLEEATGLTGVLAVSCGIDISRYIASF
                     EPRPVPRILFVGRVMSEKQLPVLLRAFAVLPRDLGARLDIVGDGDQLHELEGLARTLG
                     VDGRVTFAGYLDEARLHQAYTTASVLAMPSTAELQSIVTMEAMASGLPVVAADAMALP
                     HLVHDGENGYLFPPGDVAALAARLQDVLRAPAAKMRAMQEASLRIVAAHDMERTLDTF
                     ERLYRGLAAEPAGQGTDGEVAARHDTASIGIVGDGVPGGESTE"
     gene            complement(4703..5617)
                     /locus_tag="VFQ96_02960"
     CDS             complement(4703..5617)
                     /locus_tag="VFQ96_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0256780.1"
                     /translation="MTGELPLDPGQFLGIPIALVGALFLSAGAQLQHRGVNRVDGASS
                     ASGGLGLGQLRMLVSRPSWVVGTLMLGLAIVFQLAALVPSTLAMVQPLGALSLVVTAI
                     VNSRVSGVALNRLSIVAICLCIGGIGLFVSVTAVTTRNTNVSDRALDGILLILAVVVA
                     LAAALFVTLRHRFTAIYYVVGAGLLYGFVATLAKVVIARTVQHNPSWLLVLCVVGILV
                     AVTAGAYFVQNAYASGPPDLVIAGLTVIDPIVAVTIGLVILREAAESPLWAVALYVLS
                     GLIAICGVFLLARHHPQIHEPATPRRDP"
     gene            5687..6250
                     /gene="def"
                     /locus_tag="VFQ96_02965"
     CDS             5687..6250
                     /gene="def"
                     /locus_tag="VFQ96_02965"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0256781.1"
                     /translation="MALLPIRICGDPVLHSPAKPVHTIDSGVTDLVHDMTETMREAPG
                     VGLAAPQVGVALRLFVYHWVDGEGVTHEGEAINPTLWITPVPAVEATEDDEEGCLSFP
                     GERFALTRAARARLIATRLDGSNAEVDATGWLARIFQHEYDHLDGVLYVDRLRGAHRK
                     AALKARRRHEWGVPGLEWLPGRDHPEG"
     gene            complement(6279..6351)
                     /locus_tag="VFQ96_02970"
     tRNA            complement(6279..6351)
                     /locus_tag="VFQ96_02970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6316..6318),aa:Asn,seq:gtt)
     gene            complement(6398..8482)
                     /gene="dnaG"
                     /locus_tag="VFQ96_02975"
     CDS             complement(6398..8482)
                     /gene="dnaG"
                     /locus_tag="VFQ96_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU0256782.1"
                     /translation="MPGMIRQSDVEEVKARTNLADVIGDYVVLKPAGVGSLKGLCPFH
                     DEHSPSFNVRPQAGFYHCFGCGESGDVYSFLQRIDGLSFVEAVERLAARLGYQLTYDE
                     TAGARSDRSGRARLLAANTEAEKFYRASLLRPEAREGRRFLGGRAFDAAAAERFGVGY
                     APSGWDALTRHLTALGYSKEELVQAGLASLGQRGVYDRFRGRVVWPIRDITGQTVGFG
                     ARRLGADDTGPKYLNTPETAIYHKSQVLYGLDVAKRQITRTHRVVVVEGYTDVMACHL
                     AGVETAVATCGTAFGADHAGILRRLLGDEGSAEVIFTFDPDEAGQKAAVHAFSQSHDL
                     AAGRYVAVGPRGLDPCDLRIQAGDVAIRDMIEAKKPLFDFMIRRIVLPFDLDTVDGRA
                     AALMAARPIVASVGDPILRRGYVRHLAGLLGMELDEVAGAVRQVGTPRRGRAVPRRTR
                     GSAAPAGLAEAGPPASRISRPSPHGTASQGLGPLVNAPAGDSRRDGGAGTLRPGEAVP
                     GGAVPGEPGLGAPGGRDAGAEARRPAVPILTSRQGLPRDPDTRLERDALQALLQRPDA
                     PAAELLGRALRSRVDNPSLAMLRDAMLARSGGAPTPDWLADVLHDLPEELRPLAEDLA
                     AAPLPGRDSDDVRAYCQGMARALVARDLQLAKRDLVRRLQRLDPDDVAGRRGLQRQIA
                     DAEVERRRLRDE"
     gene            complement(8493..8984)
                     /locus_tag="VFQ96_02980"
     CDS             complement(8493..8984)
                     /locus_tag="VFQ96_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEU0256783.1"
                     /translation="MDVPPPPPDAWSKDEAGRAEPSASEGATPGQRPARPDSRRPSPA
                     EAFADRLDEGRFFPALFDFTFTRFATRRLAGPLYAVGLAVILLGVVYGFIISFAAAVA
                     TGVSWGVLLFLFGVILTVVAAMLAILLLRVGIEMVVAIVAIAQQTRARAGLSRRGQRR
                     EGH"
     gene            complement(8995..10272)
                     /locus_tag="VFQ96_02985"
     CDS             complement(8995..10272)
                     /locus_tag="VFQ96_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEU0256784.1"
                     /translation="MAAEPGAGATARPPGRYGPQDRQRWLPEDHANRRSDFARDRARL
                     VHSSALRRLAAKTQVVSPTSGVGFARNRLTHSLEVAQVGRELAVNLGVEADVVDTACL
                     AHDIGHPPFGHNGERALNEFARGIGGFEGNAQTLRLLTRLEPKVFATDGTPYGLNLTR
                     ACLDASCKYPWPVDDAALARGERVKFGYYADDAAAFAWLRQGAPGRRRCFEAQIMDLS
                     DDIAYSVHDFEDAVFGGYVDVTVLGTRDEEGLVGAIHAWVGNDFTHDGLRAALERLKG
                     LEVWLHRWNGGRLAQARLKNLTSQLIGRFAGAAIEATRRAQPADSLSRYHADVIVPAG
                     IRAEIAVLKGIVATFIMSSDTRQPLYARQRELILDLCRVLLAGAPESLEPAFAEEWRG
                     AVDDAGRRRVVVDQVASLTDQTALSWAERLVRG"
     gene            complement(10262..11413)
                     /gene="dusB"
                     /locus_tag="VFQ96_02990"
     CDS             complement(10262..11413)
                     /gene="dusB"
                     /locus_tag="VFQ96_02990"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU0256785.1"
                     /translation="MVGDIALDVPVELAPMAGITNAAFRRLCQEFGVGLYVSEMTTAR
                     ALVTRNAKSMQLIRHHESEKTRSVQLYGVDAASIREAVTMLVAEDRADHIDLNFGCPV
                     PKVTRRGGGAALPWKLGLFRSIVEAAVQAAGPVPVSVKMRKGIDADHLTYLEAGTIAE
                     GSGVASIALHARTAADFYSGHSDWSAIARLKERIAGIPVFGNGDIWSADDALAMVDET
                     GCDGVVVGRGCLGRPWLFAELAAAFRMRRGELRADEARARIRHPSLGEVGRALARHAR
                     LLVEFFGDEDQGCRDIRKHVAWYFKGYPVGGEVRSRMAMVRSLAELDDLIGLLDPDQP
                     YPGAGAEGPRGRAGTPRKPHLPEGWLASHELGGAERAGLTEGAGDTSGG"
     gene            complement(11827..12546)
                     /locus_tag="VFQ96_02995"
     CDS             complement(11827..12546)
                     /locus_tag="VFQ96_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEU0256786.1"
                     /translation="MSTPIKVDIWSDVACPWCYIGKRKFEAGAGLFAGEGQGRAVDVE
                     YHSFELAPDTPVDFEGSEFEYLSERKGIALDQVTQMLGRVSSIAESVGLNYDFPAVQH
                     TNTVKAHQALHFAKAHGKQAQLKERLLKAYFVEGGHVGHIDTLVGYGAEVGLDPEELR
                     RSLESDEYLAAVRADQTKAQEYGIQGVPFFVIDGKYGVSGAQEAATFAQVLGQVWEER
                     SADETSEPAAEPAAAAREGAR"
     gene            complement(12594..13394)
                     /locus_tag="VFQ96_03000"
     CDS             complement(12594..13394)
                     /locus_tag="VFQ96_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU0256787.1"
                     /translation="MKPYTHRDAVPFRPLDWTGVYPPAFAAGTVPAHVAIVMDGNGRW
                     ANRRGLPRVEGHRAGEAALLDVVAGAIQAGVRNLSVYAFSTENWRRSPDEVRFLMGFN
                     REVLHRRRDQLNEWGVRVRWSGRTPRLWRSVVKELRFAEELTRTNTALTLTMCVNYGG
                     RTELVDAARALAADAAAGRLAPRAVTERTLRRYLYQPDMPDVDLFVRSSGEQRTSNFL
                     LWQSAYAEMVFLDTLWPDFTRRELWSAIERYGARHRRFGGAVDTPRVT"
     gene            complement(13391..>13981)
                     /gene="recO"
                     /locus_tag="VFQ96_03005"
     CDS             complement(13391..>13981)
                     /gene="recO"
                     /locus_tag="VFQ96_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0256788.1"
                     /translation="EPFMVADLQLYRGRSLDIVTQAVTLATYGTRIAGDYRLYTVANS
                     MAEVAARLTEAAAGVQQYLLLVGALRSLSRGEHDPAATLDSYLLRALSVAGWAPSFDA
                     CARCGAVGPCRHVVVQMGGAVCDACAPPGAPRLDEATVRLLGTLLAGDWAEVDGAGEQ
                     TRQAAGGIVSAYAQWHMEKGLRSLRLVDRRPDEEAV"
BASE COUNT         2281 a         5015 c         4942 g         1743 t
ORIGIN      
        1 tcgccgcccg ccacccagca cgacccggcg cacggcggcc cggggcactg atcctcgccg
       61 cctcgtacga cgccgcgaga gccgcccaag acctaccccg cccggcacat ggccgggcag
      121 aggagaaacg gagaacgatg cccgacaaca ccgacggaag agaggccggg aacgacgaag
      181 ggcaagcccc ctacaccggg gcgcttgcca ccttcgagga gttgatcaac gaatcccccg
      241 ccccgacccc cgacttcacg accgtgttcc gcggctacga caagagcgag gtcgaccagg
      301 cgctcggcgc ggtgacggca cggctggaac gccaagagaa agagtcgcgc cgagccctgc
      361 gcgaggcgcg gcaggccgaa gacaagtggg aggcggccca ggcggggctc gagaagatgc
      421 gggccgacct cacggtcgcg aaggcacagc tggcccaggc taagcaagag gctagggcgt
      481 tggccgaaga gatcaccggc gccgacggcg acggcagccc gacaaaggag cgcttcgaga
      541 agatcctgcg cgtcgccgag gatcaggcgg cagaactcgt cgagaacgcc gccagccagg
      601 cccggcagat gctggagacg acgcgtcgcg acatcaaggt cggcaaagac caggccaaaa
      661 acgaggccga ggagattctg gagcgggcac gcaacgacgc gcggcaagag atcctgcgcg
      721 cccagcaaga cgcgacccag gcccgcacgc aggccgaacg cgaggctgcc gcggcgacgg
      781 agaagacccg gcaggcagaa caggaatccg ccgccgtgcg cagcgccgcc gagcgcgacg
      841 ccgcggccct gcgggcgcag gtcacccggc agaccgaggc ggcgcgcggc gaggcggaga
      901 ccgccatccg ggagctgcag gttcgcacgg ccgagttcga agaggcgttc agcaggaggc
      961 aggcagacgc gcagcaggag ttcctcaccc tgcacgagca ggccgtcgcc cacgccgaac
     1021 ggatcaccga cgacgccaac cggcaggtcg ccgacgccct cgatcacgcc cggcgcacca
     1081 cggcgcgcgc cgacgacttc gaccgcctca cccgcgcacg cgccgagaag atcctcgccg
     1141 gcgcgaaaga ccgtgccgcg gccgacctcg acaaggctcg cgtgaaggcc gagaagattc
     1201 ttggcctgat ctcagatcac gcgctcaccg ttctgcggga ggccgaagac cgtgcccggc
     1261 agctgcgctg ggaacaacac cagacggaaa gcttgacggc ccagatccgt gacctcacga
     1321 ccccgggggc cgccgcccag ccgccggcac cagagaagcc cgctgacagc gcggcacgcg
     1381 cggtggccac gcctcccgcc ccgggggacc acaccgctcc ccgggctgcg accccggaga
     1441 cgcccggcga gcacgctgcg aagacgaacg atgccgcgag cccggccgaa ggatccgggc
     1501 ggcgaaccgg cccgaaaaca agccccgcgg gcggcgggga aggcaaggtg accccgacga
     1561 gcccggccga aggagtcgcc ggcatgcggg agcgtgcgaa ggccacccgc gacgactccg
     1621 cgctcaccgt gccgataaca cgcccgaagg cctgaccggc accgacgagc gggccgcttg
     1681 atccgaggcg cggaggggca ggtcgctgat cctcgcgccg ccgcaaggcc gaggcggccg
     1741 ctgccctcgc ccgagcggca cgtggctggc gaccgcgcgg gtcagcgtag gcgaggcctg
     1801 cccgcccggc ccttcgcctt cgtcgccggc cgccgcggca cccaggcgaa cacccgcgat
     1861 acatgaccgg gcgcgcggtg ctgttcctcg acggccgata cgcgtggcac ctccgacgcc
     1921 gcctcggcgg tcggcatcac gacggtggtc accggcacgg ccacgcgcgc gctgtcaggc
     1981 gcctggctcg gcgccgcagc agccggcacc gacgcgcgcg ccgacgttcc gcggccgcgc
     2041 gccacgacac accaggtgac cccggccaca gtcagagaaa gccaggcgat cgccgaggcc
     2101 gccaccacgg cgacgcccga acgaccggcg gcgaaatagg cgaaggtgcc cacggcgagg
     2161 atgccggcga ccccgacgag cgcccaccgc gcggcggtgc gaggcaggct cggccggcct
     2221 gccatgccac gaagacgcgc cgccgccacg ccgaaggagc cccccagcag cgcggcgacg
     2281 gccgtgaacc acaagacggg atagaagcgc aggagcaccg gcggccacag catgccggcg
     2341 gccgcggcga gggaggccgc caaagcgcac cagaggatgc cgttcgacgg ccgtgcaccg
     2401 gggagcacgc ccgcggcggc gaaaccaccc acgccggcga gccccgcgac ggtgacggcg
     2461 gccgccacct cccccaccgg cagcagatac cgcccgtcgg ccgaccacgg caagaccgca
     2521 agcacggcga cagccgtgac gcaacatagg gcggcagcca ggtctgcggc gaacgacgcg
     2581 ccgagaacgc cgcccgcacc ccccgacgca ccgttcgaaa gcgaacccac gacgagcggc
     2641 ctcgacgcgg ccgggccgcc cagccgacct ctgtcggtga cggcgccgtg ccggcgcagc
     2701 acggcggccg cggcgatcgc gatggccgcc tccacaagga gcgccacccg ccaccccagg
     2761 cccacgggca agacggcggt cggcaccccg ccgatgagga cgcccgcccc gacgagggcc
     2821 gccacccacg cctccgcgtc gccccgccgc gtgcggaatt tacgggccag aacagacagc
     2881 gagacgccgg tgagaacaag ggacaaaaac ccgaggcagg cggcggccgc gacgagcgcc
     2941 acggcgccgc ctacaagcgc ggcggccgcg gcgacgacgc cggcgatgcc gagttccgcc
     3001 acaggccggc gagcgcttgc ggcacctcgc gcccgggctg ccgacacggc ggcaccgagc
     3061 agaaaggcgg ccgcggcggc cggcggcccg gccgcccaca ccgtgacacc gaagcccgcc
     3121 gcaacggcga gcggaccgaa agccacgacg gtgagggagg tccagtcgtc ttccgcaaca
     3181 tcacccacgc tataagccta aggagcatgg gctcggcgtg cgctgtgcgt cacccctgtg
     3241 tcgcccgaag tgcccgccgc ctggccggca cgcggaattg ctgcctattc ggtgctctcg
     3301 ccgccgggca cgccgtcgcc gacgatcccg atcgaggcgg tgtcgtgacg cgcggcgacc
     3361 tcaccgtctg tgccttggcc ggcgggttcg gccgccagac cacggtagag gcgttcgaac
     3421 gtgtcgaggg tgcgctccat gtcgtgtgcg gcgacgatgc gcagcgacgc ctcttgcatg
     3481 gcgcgcatct tcgcggcggg ggcacgcagc acatcttgca gccgggcagc caacgccgca
     3541 acgtcgccgg gcggaaacag gtagccgttc tcgccgtcgt gcacgagatg cggcagcgcc
     3601 atcgcgtcgg cggcgacgac cggcagcccg gaggccatcg cctccatcgt cacgatggat
     3661 tgcagctccg ccgtcgaggg catcgcgagc acactcgccg tcgtgtatgc ctgatgcagt
     3721 cgcgcctcgt ccagatagcc cgcaaaagtg acccggccat cgacgcccag agtgcgggcg
     3781 aggccctcca actcgtgcag ctggtcgccg tcaccgacga tgtccagcct cgctccgaga
     3841 tccctcggca gcacggcgaa ggcccgcagc agcaccggaa gctgtttctc gctcatcacc
     3901 cggccgacga agagaatccg cggaaccggt cgcggctcaa aacttgcgat gtagcggctg
     3961 atgtcgatgc cgcacgagac ggcgagcacg ccggtcagcc cggtggcctc ttcgagaaag
     4021 tgcgcggccg tcggcgtggg cgaggtgacg acgtcggcgc gggcgaagct gcgcgcggcc
     4081 gcccgccagg ccatgcggat cgagagcctg cgcagcgcct tcggtatggg cgcgtggtcg
     4141 aacaggtttt ccggcatgaa atggttcgtg ccgacgacgc ggatccctcg acgtttggcc
     4201 tcgatagaaa ggccccggcc ggtgatgata tgcgactgga aatgcaccac gtcgggcatg
     4261 acctcgtcga ggatgcgggc gctgttctct ttgatgcgcc acgggagggc gaagcgcagc
     4321 caggagggcg aataccaccg ccagctgagc aggcggtgcg ccgtgactcg ctgcccgtcg
     4381 tactcctcgc tccaggtgcc atgccgtcgg ctcgcggccg gcgccacgat atgcacgtcg
     4441 tggccgcgcg ccgccatgcc cgaggcgagg tgctgggcga agcgggccgc gccgttcacg
     4501 ttcggcggaa acgtgtcggc gccgatgacg atcgtcagtc gccgggacgc ggtcggcctg
     4561 tcgtgccggc ccgggtcgtg cggttctgac aacacggcat ccttccgaat gatccggccg
     4621 cccggcgggc gccgtgggca ggccgcccca caccggccgg caccctccag cgtcggtcag
     4681 gggttcagtc tatgcgacgc cttcaggggt cgcgacgagg cgtggccggc tcatggatct
     4741 gcggatgatg gcgggccaaa agaaagacgc cgcagatcgc gatgaggccc gagagcacgt
     4801 agagcgcgac ggcccacagg gggctttcgg cggcctcccg caagatgacg aggcctatcg
     4861 tgacggcgac gatcgggtcg atgacggtca ggcccgcgat cacgaggtcc ggcggccccg
     4921 aggcgtaggc gttctgcacg aaatacgccc cggccgtcac ggcgacgaga atgccgacca
     4981 cgcacaagac gagcagccac gacgggttgt gctgcaccgt gcgggcgatg acaaccttcg
     5041 cgagggtcgc gacgaacccg tagagcagcc cggcgccgac gacgtagtag atcgccgtga
     5101 aacggtgacg cagggtgacg aacagcgcgg cggcgagggc gaccacgacg gcgaggatga
     5161 ggaggatccc gtcgagcgcg cggtcagaga cgttcgtgtt gcgggtcgtc accgccgtga
     5221 cgctgacgaa gaggcctatg ccgccgatgc acaggcagat cgccacgata gagagtcggt
     5281 tgagcgcgac gccgctcacc cgggagttga cgatcgcggt gacgacgagc gagagggcgc
     5341 cgagcggctg caccatcgct agcgtcgagg gcacgagggc ggccagctga aagacgatcg
     5401 cgaggccgag catcagggtc ccgacgaccc acgagggccg cgagacaagc attcgcagct
     5461 ggccgaggcc gaggccgccg ctcgccgagg aggcgccgtc gacccggttg acgccccggt
     5521 gctgcagctg cgcgccggcg gaaaggaaca acgccccgac gagggcgatc gggatgccga
     5581 ggaactggcc cgggtcgagc gggagttcgc cggtcacggc cgcccgttca ccgatgcggc
     5641 ccgcccggcg ggggcgccgg gcacagcgag ccgatactgt tgagcaatgg cgcttctccc
     5701 gatccggatc tgtggagacc ccgttctgca ttctcccgcg aagcctgtcc atactatcga
     5761 ctcgggcgtg accgacctcg tgcacgacat gacagagacg atgcgcgagg cgcccggcgt
     5821 gggcctcgcc gcgccgcagg tgggcgtggc cttgcgcctc ttcgtctacc actgggtaga
     5881 cggcgagggc gtcacccacg agggtgaggc gatcaacccg accctgtgga tcacacccgt
     5941 gcctgcagtc gaggcgacgg aagacgacga ggagggctgc ctgtcgttcc ccggcgagcg
     6001 tttcgccctg acgcgtgcgg cgcgggcgcg tcttattgcg acaaggctcg acggcagcaa
     6061 cgccgaggtg gatgcgaccg gctggctggc gcgcatcttc cagcatgagt acgaccatct
     6121 cgacggcgtg ctctacgtcg accggcttcg cggcgcacac cgcaaggcgg cgttgaaggc
     6181 gcgccgtcga cacgagtggg gcgtaccggg cctcgaatgg ctgcccggcc gcgaccaccc
     6241 cgagggctga ccctgcctac ggccagaatg ctctgaagct cctcgagtag gactcgaacc
     6301 tacaacctgc cggttaacag ccggctgctc tgccaattga gctatcgagg aatacggcga
     6361 cgtgcagtaa ctttatcaaa ccttcggcgc ccgccgctca ctcgtcgcgc aggcgccgtc
     6421 gctccacctc ggcgtcggcg atctggcgct gcaagccccg ccggcccgcc acatcgtcgg
     6481 ggtcgaggcg ttgcagacgc cgcacaaggt ctcgtttggc gagttgcagg tcgcgggcga
     6541 cgagcgcacg ggccatgcct tggcaatagg cgcgcacgtc gtcggaatcc cggccgggca
     6601 agggtgcggc ggcgaggtcc tcggcgagcg ggcggagttc ctcaggcagg tcgtggagga
     6661 cgtcggccaa ccagtccggc gtcggcgcgc cgcccgagcg agccagcatc gcgtcgcgca
     6721 gcatcgccag cgacgggttg tcgacccggc ttcgcagggc gcgccccagg agctcggcgg
     6781 ccggcgcgtc ggggcgctgc aagagggcct gaagcgcgtc tcgctccagg cgggtgtcgg
     6841 ggtcacgcgg caaaccctgt cgtgaggtga ggatcggcac ggcagggcgt ctggcctcgg
     6901 ccccggcatc acggccaccc ggggcgccga ggccgggttc ccccggcacg gcaccgcccg
     6961 gcacggcctc ccccggccgt agagtgccgg caccgccgtc gcgccgcgag tcgcccgccg
     7021 gagcgttcac gagcgggccg aggccctgcg atgcggtgcc gtggggcgac ggccgagaga
     7081 tacgcgaggc cgggggcccc gcctccgcaa gaccggccgg ggcggcggag cctcgggtgc
     7141 gacgcggcac ggcacggcca cgccgggggg tgccgacctg tcgcacggcg cccgccacct
     7201 cgtcgagctc catgccgagg aggcccgcga ggtgacgcac gtagccgcgg cgcaggatcg
     7261 ggtcgccgac ggaggcgaca atggggcggg cggccatgag ggcggcggca cgaccgtcga
     7321 ccgtgtcgag gtcgaagggc agcacgatgc gccggatcat gaagtcgaag agaggcttct
     7381 tggcctcgat catgtcgcgg atcgcgacgt cgcccgcctg gatgcgcagg tcgcacgggt
     7441 cgaggccgcg agggccgacc gcgacgtaac gcccggcggc caggtcgtgc gattggctga
     7501 aggcgtgcac ggcggccttc tgcccggcct cgtcggggtc gaaggtgaag atcacctcgg
     7561 cgctgccctc gtcgccgagc agacgccgca ggatcccggc gtggtcggcg ccgaacgccg
     7621 tgccgcatgt ggcgacggcc gtctccacgc cggccaggtg gcaggccatg acgtcggtgt
     7681 agccctcgac gacgacgacg cgatgcgtgc gggtgatctg ccgcttcgcg acgtctaacc
     7741 cgtacagcac ctgagacttg tggtaaatcg ccgtctcggg cgtgttgaga tacttcggcc
     7801 cggtgtcgtc ggcgccaagg cgcctcgcac cgaaccccac ggtctggccg gtgatgtcgc
     7861 ggatcggcca cacgacgcgg ccgcggaacc ggtcgtagac gccgcgctgg ccgagggagg
     7921 ccagacccgc ctgcaccagt tcctccttgc tgtagcccag agccgtcagg tgccgggtca
     7981 gggcatccca gccggagggc gcgtagccca ccccgaagcg ttcggccgcg gccgcgtcga
     8041 acgcgcggcc gccgagaaaa cgccggccct ccctggcctc cggtcgcagg agcgaggcgc
     8101 gatagaactt ttcggcctcg gtgttcgcgg ccagaaggcg ggccctgccg ctgcggtcgc
     8161 tgcgcgcgcc ggccgtctcg tcgtaggtga gctggtagcc gagccgggcg gcgagccgtt
     8221 ccaccgcctc gacgaagctc aagccgtcga tacgttgcag aaaactgtag acgtcgccgc
     8281 tctcgccgca tccgaaacag tggtagaagc cggcctgggg gcgcacgttg aagctggggc
     8341 tgtgctcgtc atggaacggg cagaggcctt tgagtgagcc gaccccggcg ggtttcagca
     8401 cgacgtagtc gccgatcacg tcggcgaggt tcgtgcgtgc cttgacctct tcgacgtcag
     8461 actgtcggat catcccgggc acgagtcgcc cctcagtgcc cttctcgccg ctggccgcgg
     8521 cgggagagcc cggcacgtgc ccgggtctgt tgcgcgatcg cgacgatggc gacgaccatc
     8581 tcgatgccga cccgcaataa gaggatcgcg agcatcgcgg cgacgacggt gaggatcacg
     8641 ccgaaaagga aaagcagcac accccacgac acgcccgtcg cgacggcggc agcgaaactg
     8701 atgatgaacc cgtagacgac ccccaagagg atgacggcga gccccacggc atagagcggc
     8761 cccgcgagcc gcctcgtcgc gaagcgggtg aaggtgaagt cgaagagggc cggaaagaac
     8821 cggccttcgt cgagccggtc ggcgaaggcc tcggcgggcg agggccgacg cgaatccggc
     8881 cgcgccggcc gctgccccgg cgtcgcaccc tccgacgccg aaggctccgc acgccccgcc
     8941 tcgtctttcg accaggcgtc gggcgggggc ggtggcacat ccatgtcgcc caccttaacc
     9001 tcgcaccagc cgttcggccc acgacagcgc ggtctggtcg gtgaggctcg ccacctggtc
     9061 gacgacgacc cgccggcggc ccgcgtcgtc gacggcgccg cgccactcct ccgcgaacgc
     9121 cggttccaac gattccgggg cgccggccag gagcacccgg cagaggtcga gtatgagttc
     9181 ccgctgccgc gcatacagcg gctgccgcgt gtcgctcgac atgatgaagg tcgcgacgat
     9241 gcctttcaag acggcgatct ctgcgcggat gccggccggc acgatcacgt cggcgtggta
     9301 gcggctcagg ctgtctgccg gctgcgcccg ccgggtcgcc tcgatggcgg cgcccgcaaa
     9361 ccggccgatg agctggctcg tcaggttctt caggcgggcc tgggcgaggc ggccgccgtt
     9421 ccagcggtgc agccacacct cgagcccctt caggcgttcc aaggcggcgc gcagcccgtc
     9481 gtgcgtgaag tcgtttccca cccacgcgtg aatcgcgccc acgagcccct cttcgtcgcg
     9541 ggtaccgaga accgtgacgt cgacgtagcc accgaagacg gcatcttcga agtcgtgcac
     9601 cgagtaggcg atgtcgtcgg agagatccat gatctgcgcc tcgaagcaac gcctgcggcc
     9661 gggggcgccc tggcgcagcc aggcgaaggc ggccgcatcg tcggcgtaat agccgaactt
     9721 gaccctctcg ccgcgggcga gcgccgcgtc atcgaccggc cacgggtact tgcaactcgc
     9781 gtcgaggcac gccctcgtca ggttcagtcc gtacggggtg ccgtcggtcg cgaaaacctt
     9841 cggctcgagc ctcgtcagaa ggcgcaacgt ctgcgcgttg ccctcgaacc cgccgatccc
     9901 gcgggcgaac tcgttgagag cccgttcgcc gttatggccg aacggcgggt gcccgatgtc
     9961 atgggcaagg catgccgtat cgacgacgtc ggcctcgaca ccgaggttca cagcgagttc
    10021 ccggccgacc tgggcgacct cgagcgaatg agtgagccga ttgcgggcaa aacccacccc
    10081 ggatgtcggg ctgacgacct gggtcttggc ggccaggcgc cgcaacgcgc tggaatgcac
    10141 caggcgggcg cggtcgcggg cgaagtcgct gcgccggttg gcgtggtctt ccggcagcca
    10201 cctctgccga tcctgagggc cgtagcggcc cggcggccgc gccgtggctc ccgcccccgg
    10261 ctcagccgcc actcgtgtcg cccgcccctt cggtgagccc tgcccgctcc gcgccgccca
    10321 gttcgtggct ggccagccag ccctcgggca ggtggggctt gcgcggcgtg ccggcgcggc
    10381 cgcgtggccc ctcggcgccc gccccggggt agggctggtc aggatcgagc agcccgatga
    10441 ggtcgtcaag ctcggcgagg ctgcgcacca tcgccatacg cgagcgcacc tcgccaccca
    10501 cgggatagcc cttgaaatac caggcgacgt gcttgcgaat gtcgcggcag ccttggtctt
    10561 catcgccgaa gaactccacg agaagccgcg cgtggcgggc aagcgcacgg cctacctcgc
    10621 cgagtgatgg gtgccggatg cgcgccctgg cctcgtcggc acgcagctct ccccggcgca
    10681 tgcgaaacgc cgccgcaagc tccgcgaaaa gccagggccg gccgaggcag ccgcggccga
    10741 cgacgacgcc gtcgcagccg gtctcgtcga ccatggccaa cgcgtcgtcg gccgaccaga
    10801 tgtcgccgtt gccgaagacc gggatgccgg cgatgcgttc tttgaggcgt gcgatcgccg
    10861 accagtcaga gtgtccggaa tagaagtcgg cggcggtgcg ggcgtgcagg gcgatggagg
    10921 cgacgcccga gccctcggcg atcgtgccgg cctcaaggta ggtgagatgg tcggcatcga
    10981 tgcccttgcg catcttgacc gacacgggca ccgggccggc cgcctggacg gccgcctcga
    11041 cgatgctgcg gaacaacccg agtttccacg gcagcgcggc gccgcccccg cgcctcgtga
    11101 ccttgggaac ggggcagccg aagttcaggt cgatgtggtc ggcccggtct tcggcgacga
    11161 gcatcgtcac cgcctcgcgg atgctcgccg catcgacacc gtacagctgc acggaacgcg
    11221 tcttctccga ctcgtggtgc cggatcaact gcatcgactt cgcgttgcgc gtgacgagag
    11281 cccgcgcggt cgtcatctcg ctgacgtaaa gccccacgcc gaactcttgg cagagccgcc
    11341 ggaaggcggc gttcgtgatt cccgccatgg gcgccagctc gaccgggacg tcgagcgcga
    11401 tgtcgccgac catgaccggg ccgcgccccc ggtgcgacag gtcgcgcccg gccgcgtcgc
    11461 gccccgccgt cggcgcatgc ccgctccggg ccgaggcgtc ggcggtggcg ccggccctgg
    11521 agtcggcgag cggagcaccc ggcagccggg cgtcacgaac ggtggtaggc atcgccccca
    11581 ttctcgcaga cccgggtacg gctcagcggg cgaaggccga tccggcctcg acgggctcgg
    11641 agcccgccgc aggcgacgca ccttccacgg tgtgggcgag cgcagaaaca gcgcctgacg
    11701 ccgacgggtc aggctctgcc tcgcctgccg ccgtgacgcc cgacgccccg gctgtggcgc
    11761 ccgggatgaa gcacacgccg tcgacgcaca caacggcgtc ggggtcgccg agcggcgtca
    11821 ggccgttcac cgggcgccct cccgcgcagc cgcagcgggc tccgccgccg gctcgctcgt
    11881 ctcgtcggcc gaccgctcct cccacacctg gccaagcact tgggcgaacg tggcggcctc
    11941 ttgcgcgccc gagacgccat acttgccgtc gatcacgaag aagggcaccc cctggatgcc
    12001 gtactcctgc gccttcgtct ggtcggcgcg caccgcggcg aggtattcgt cggattccag
    12061 cgagcgacgc agctcttctg gatcgagccc cacctcggcg ccgtagccga cgagcgtgtc
    12121 gatgtggccg acgtggccgc cctcgacgaa gtacgccttc agaaggcgct ccttcagctg
    12181 ggcttgcttg ccgtgcgcct tggcgaaatg gagcgcttgg tgtgccttga ccgtgttggt
    12241 gtgctgcacc gccggaaagt cgtaattcaa cccgacgctc tcggcaatgg aagagacccg
    12301 gcccagcatt tgggtcacct ggtcgagcgc gatccccttg cgttccgaga ggtattcgaa
    12361 ctcgctgccc tcgaagtcga cgggggtgtc gggagccagc tcgaaggagt ggtactccac
    12421 gtcgacggcc cggccctgcc cctcccccgc gaacagcccg gcgcccgcct cgaacttgcg
    12481 cttgccgata tagcaccacg ggcatgccac gtcggaccag atatcgacct tgatcggtgt
    12541 gctcataccg gggagaacgg acacgccggc ccggtcattc ccgcccgcgg gcctcaggtg
    12601 acgcgcggcg tgtcgacggc gccgccaaac cggcggtgcc tagcaccgta tcgctcgatc
    12661 gcgctccaca gctcccgcct cgtgaaatcg ggccatagcg tgtcgaggaa gaccatctcg
    12721 gcgtaggccg actgccagag aaggaagttg ctggtgcgtt gctcccccga actgcgcacg
    12781 aaaagatcga cgtcgggcat gtccggctga tacaggtagc ggcgcagggt gcgctcggtg
    12841 accgcccgcg gcgcaagccg gccggcggca gcgtcggccg cgagcgcccg ggcagcgtcg
    12901 accaattcgg tgcgcccgcc gtagttcacg cacatcgtca acgtgagggc cgtgttcgtg
    12961 cgcgtgagct cttcggcgaa gcgcaactcc ttcacgacgc tgcgccagag cctgggggtg
    13021 cgacccgacc agcgcacccg cactccccac tcgttcagtt ggtcgcgacg gcggtgcagc
    13081 acctcacggt tgaaccccat caagaagcgc acctcgtcgg gtgagcggcg ccagttctct
    13141 gtcgagaacg catagacgct caggttcctg acgccggcct gaatcgcacc ggccaccacg
    13201 tcgagcaggg ccgcctcgcc cgcccggtga ccttcgaccc ggggcaggcc ccgccggttc
    13261 gcccagcggc cgttgccgtc catgacgatc gcgacatgtg ccggcaccgt acccgccgcg
    13321 aaggccggcg ggtagacacc ggtccagtcc agaggccgga acggcaccgc gtcccgatgg
    13381 gtgtaaggct tcataccgcc tcttcgtcgg ggcgacggtc gacgaggcgc aacgagcgca
    13441 ggcctttctc catatgccac tgcgcgtatg cggagacgat gccgcctgcc gcctgccggg
    13501 tctgctcgcc ggcaccgtcg acctccgccc agtcgccggc gaggagcgtg cccagcaggc
    13561 gtaccgtcgc ctcgtcgagc cgcggcgcgc cgggcggcgc gcacgcgtcg cagaccgcgc
    13621 cgcccatctg caccacgacg tgccggcacg gccccacggc gccgcatcgg gcgcacgcgt
    13681 caaagctcgg cgcccacccc gcgaccgaga gggcccgcag caggtaggag tcgagcgtcg
    13741 cagccggatc gtgctcgccg cgggaaagcg agcgcagggc tcccacgagc agcaggtact
    13801 gttgcacgcc ggccgcggcc tccgtgagcc gcgccgcgac ctccgccatc gagttcgcga
    13861 cggtgtacag gcggtagtcg ccggcgatcc gcgttccgta cgtcgcgagc gtcaccgcct
    13921 gggtgacgat gtcgaggctg cgcccccgat acagctgcaa gtcggccacc atgaacggct
    13981 c
//
LOCUS       DASTNL010000090         7463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1548566, whole genome shotgun sequence.
ACCESSION   DASTNL010000090 DASTNL010000000
VERSION     DASTNL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548566"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFQ96_03010"
     CDS             complement(<1..227)
                     /locus_tag="VFQ96_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HEU0256789.1"
                     /translation="MALDHVRRLAALAAAACLVPLVAGCTATVSLPPAAHADSPDCAA
                     VTVRLPASVAGLAKRATDSQATAAWGSPPAVL"
     gene            253..1308
                     /gene="thiL"
                     /locus_tag="VFQ96_03015"
     CDS             253..1308
                     /gene="thiL"
                     /locus_tag="VFQ96_03015"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU0256790.1"
                     /translation="MSASDGTAPRAGALAETVAQLGEEAVLRRLTPLLPVSGAASLGI
                     GDDAAVVAAPDGRFVVTTDMMVEGPDFRTAWSTPFDLGWKAAATNLSDVAAMGARPSA
                     LVVALAVAPDTALRRLEDFARGLAAACLALAPSCGVVGGDLSTSVTHTITVTALGDLE
                     GRAPVTRAGARPGDVVAYCGELGLAGRGLRLLFEQGVDGNGRPDRSRAEGLRRLHRTL
                     IDAQLRPAPPIASGPVAARAGAHAMMDVSDGLAKDARRLARASGVSVIFDREALGDDP
                     EMAMRGGEDHGLLATFAPTAPLPEGFRRLGRAGPADRGARVWVGRHPYRDDGGWDPYR
                     DASRAGARDSHDTTVSP"
     gene            complement(1248..1847)
                     /locus_tag="VFQ96_03020"
     CDS             complement(1248..1847)
                     /locus_tag="VFQ96_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU0256791.1"
                     /translation="MTRIIGGYAGSLSLVVPKNGTRPTSDRVREAVFSALDARGMLPG
                     AHVLDLYAGSGALGLEAASRGAARVTLVESAAAAVRACRRNAAAVVAAAAGRVDVPTI
                     DIRATTVMRFLEREAHTGAGWNVVFLDPPYALAAGEMASALRRVIPGLAPGASIVAER
                     GVHRDAFTPPAGLEEVRQSRYGDTVVSWLSRAPAREASR"
     gene            1907..4216
                     /locus_tag="VFQ96_03025"
     CDS             1907..4216
                     /locus_tag="VFQ96_03025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0256792.1"
                     /translation="MGTPGRASPTPGETPAPAGPRSSAGAGLRDPLRGIIGGRSAGSL
                     HKAFGFETVRDLVTHYPRRYASRGELSPLAALTEGEAVTVVAEVQGVRERPMRARRGT
                     ILDVRLSDGRGSLALTFFNQPWRANELRPGVRGMFAGKVSLYRGQLQLAHPDYELFDE
                     AEGQPSETEARRWATTPIPVYAATAAIASWRIQRAVGVVLDALGELDDPVPEEIRRRH
                     GMPGYREALEMVHRPTSDAQWRRAREALRRIEALVLQTALLERRSESEASSSVARSAA
                     KGGLREKFDARLPYALTADQSAVGAEIEADLARPHPMSRLLQGDVGSGKTLVAVRAML
                     VAAESGGQCALVVPTEVLAGQHLRSIVRLLGPDLAALLEPTLVTGALSARERRTALVR
                     IASGRARIVIGTHALFGDAVVFDDLALVVIDEQHRFGVEQRDVLRTKGRRAPHTLVMT
                     ATPIPRTIAMTVFGDLDVSTLRTMPPGRRPVQTIAVPLAEHPRWEERIWARSAEEVAA
                     GRQVFLVCPAIDAEPAPGGRPDDDVPDDPDHGAPPPGRATLQAGIPLLPEGEEAPGVP
                     RSSVEQVAATARSRAELAGARIGVLHGRLPAEEKNSVMEAFAAGTIDVLVSTTVIEVG
                     VDVPNASLMVIFDADRFGLSQLHQLRGRVGRGGAAGLCLLVTRALAETPGRLRVDAVA
                     ANADGFELARIDLELRREGDVLGADQSGRRSSLRLLRVTRDGDLIAEARADAARLLAA
                     PGGLAAHPALGAALQDRLDDIERDFLARN"
     gene            4393..4875
                     /gene="coaD"
                     /locus_tag="VFQ96_03030"
     CDS             4393..4875
                     /gene="coaD"
                     /locus_tag="VFQ96_03030"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU0256793.1"
                     /translation="MNRIAVVPGSFDPVTLGHLDVIERAAKLFDEVHVVVVHNPDKDA
                     MLPVAQRVSLIEQSLDSARLGATVLVASWSIGLLVDYCSDVGAHVLVKGIRSQVDVAY
                     ETPMAIVNRNLADVETVFLLPDPAHAHVSSSLVRQVAALGGDVRPYVPTAVYEYLQAT
                     "
     gene            4872..5906
                     /locus_tag="VFQ96_03035"
     CDS             4872..5906
                     /locus_tag="VFQ96_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0256794.1"
                     /translation="MSLRGDTSVSTLGRPAAPWAKDIAARITASVETVIQGKPDAIRT
                     ALTVLLSGGHLLIEDVPGVGKTVLVKALARCVGCSVARIQFTPDLLPGDVTGVAVYDQ
                     REARFVFKPGPVFADIVIGDEINRASPKTQSALLECMAERQVTVDGATYPLAVPFTVF
                     ATQNPIEMEGTYALPEAQRDRFTARIAMGYPGRVAEVAMLGARDGAGPLERLAPVIDV
                     EDLRDVMGLVRRVYVSAPVKQYVVDIVRATRDDPEVRIGASPRATLQLVAVAKAQAVM
                     DGRDFAIPDDVDSLAVDVLAHRLILLDDVRRPDARRAAGEVVGRALRRAAAALPAAPG
                     PLPGALRHGR"
     gene            5988..7247
                     /locus_tag="VFQ96_03040"
     CDS             5988..7247
                     /locus_tag="VFQ96_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0256795.1"
                     /translation="MAGFLVLALAVLVGRQDLLVVGVLLVALPAAALVSLRLAGSLPP
                     PQLSVDPPAVPVGGTAVLTIVSGAGSAAPAEAGAWGTMRRLSTAPQAAPRAAPWRFVM
                     PDGLEDLGPAGAAADGAGGGGRITGRGTRRLIRASRRGDYSIGPLFLEVTDRFGLARR
                     RLCADGRGRVLVLPEPARLSWPVTMPPGVRENASRSGRGVGGPLEGTTARPYLPGDPI
                     RRMQWRATARFGKLMVRQEEERRRPEVWLYVDTRGRPGTAKRVRSAYAYDDESFELLV
                     RAVAAAASFSAARRCDLHVVEAGARQLFGERPGGGHASYSAARAHTLLPRELATLHPA
                     ARGEPEAALFRLTAALRRPGALVVALRPECDDLVGVLGAARPGGAATGTVVAAGPLGP
                     AVRARLLAAGWRIADVARGRAGRRVAS"
     gene            7244..>7463
                     /locus_tag="VFQ96_03045"
     CDS             7244..>7463
                     /locus_tag="VFQ96_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256796.1"
                     /translation="MTAAREGTATGAGQWRTVAALLFLIAAASTSLGGLFDGPAWWFL
                     AQAVAVVAFTVAALSRLAAPAWCASLGAA"
BASE COUNT          949 a         2700 c         2768 g         1046 t
ORIGIN      
        1 agcacggcgg gcgggctgcc ccacgccgcg gtcgcctgag agtcggtcgc ccgcttggcg
       61 agtccggcca cgctcgcggg cagacgcacc gtgaccgcgg cacagtcggg tgagtcggcg
      121 tgtgcggcgg gcggcagcga gaccgtcgcc gtgcacccgg cgacgagcgg aacgaggcag
      181 gccgctgcag cgagcgccgc gagtcggcgg acatgatcga gggccatcac ccaccaggct
      241 agcgttgtgg gggtgagcgc atccgacggc acggcccccc gagccggggc cttggccgag
      301 acggtggccc agctgggcga ggaggcggtg ctgcggcggc tgacgccgct cttgcctgtg
      361 tcgggcgcgg cgtcgctcgg catcggcgac gacgcggcgg tcgtcgccgc tccggacggg
      421 cgcttcgtcg tcaccaccga catgatggtg gaaggccccg actttcgaac ggcctggtcc
      481 acgccgttcg acctcggctg gaaggcggcg gcgacgaacc tctccgacgt cgcggcgatg
      541 ggcgcccggc cgagcgccct cgtcgtggcg ctggccgtcg cacccgacac agccctcagg
      601 cgcctcgagg acttcgcccg gggcctcgcg gcggcgtgcc tcgcactcgc accgagctgc
      661 ggcgtcgtcg gcggcgatct gtcgacctcg gtgacacaca cgatcacggt gaccgctctc
      721 ggcgacctgg agggccgggc gcctgtgacc cgtgccggcg cccggcctgg cgacgtcgtc
      781 gcctattgcg gcgagctcgg cctcgccggc cggggcctgc gcctgctttt cgaacagggc
      841 gtcgacggga acggccggcc cgaccggtcg cgtgcggagg gcttgcggcg tctgcataga
      901 accctcatcg acgcgcagtt gcggccggcc ccaccgatcg cctccgggcc ggtcgcggcg
      961 cgcgcaggtg cccacgcgat gatggatgtc agcgacgggc tggcgaagga cgccaggcgg
     1021 ctcgcccggg cgagcggcgt cagcgtgatc ttcgatcggg aggcgctcgg cgacgatcca
     1081 gagatggcga tgcgcggtgg tgaggaccac gggcttctgg cgaccttcgc cccgacggcg
     1141 ccgctgcccg aggggtttcg ccgcctcggc cgggctgggc cggccgatcg gggtgcccga
     1201 gtctgggtcg gccggcatcc gtaccgtgac gacggggggt gggaccctta ccgagacgcc
     1261 tcacgggcgg gcgcccgaga cagccacgac accacggtgt cgccgtagcg gctctggcgc
     1321 acctcttcga gcccggccgg cggggtgaag gcgtccctgt gcaccccgcg ctcggcgacg
     1381 atactcgcgc cgggtgcgag gcccgggatc acgcggcgaa gcgccgacgc catctcgcct
     1441 gccgcaagcg catacggcgg gtcgaggaac accacgttcc acccggcacc ggtgtgcgcc
     1501 tcccgttcga ggaagcgcat caccgtggtc gcccggatgt cgatcgtcgg aacgtccacc
     1561 ctgccggccg cggccgcgac gaccgccgcg gcgttgcgcc ggcatgcccg caccgccgcg
     1621 gccgcgctct cgacgagcgt cacccgggcg gctccgcggc tcgcggcctc gagccccagc
     1681 gccccgctgc cggcgtacag gtcgagcacg tgggcgcccg gcagcatgcc gcgggcgtcg
     1741 agtgccgaga acaccgcctc ccgcacccgg tcgctggtgg gtctcgtgcc gttcttcggc
     1801 acgacgaggc tcagtgagcc cgcgtatccg ccgatgatcc tcgtcactcc accacggtag
     1861 cgacaccgcg gccggggctg tcggcggccg accgtagact cgcagcatgg gcaccccggg
     1921 ccgtgcatcg ccgacgcctg gcgagacccc ggccccggcc ggcccgcgtt cgtcggcggg
     1981 agcgggtctt cgcgacccgc tgcgcggcat catcggcggc aggagcgccg gctcgctgca
     2041 caaggccttc ggtttcgaga ccgtgcgcga ccttgtgaca cactatccgc ggcgttacgc
     2101 cagccggggc gagctctcgc cgctcgccgc gctcaccgag ggcgaggccg tgacggtcgt
     2161 ggccgaggtg caaggcgtgc gggagcgacc catgcgggcc aggcgcggca cgatcctcga
     2221 cgtgcgcctc tccgacggcc ggggcagcct cgcgctgact tttttcaacc agccgtggcg
     2281 ggcgaacgag ctacgcccgg gggtgcgcgg catgttcgcc ggcaaggtct cgctgtatcg
     2341 cggccagctg cagctcgccc accccgatta cgagctcttc gacgaagcgg aggggcagcc
     2401 gtcggagacg gaggcgagac ggtgggcgac gacgccgatc ccggtctacg ccgcgaccgc
     2461 cgcgatcgcc agctggcgga ttcagcgcgc ggtcggcgtg gtcctcgacg ctctggggga
     2521 gctcgacgac ccggtgcccg aagagatccg ccggcggcac ggcatgcccg gctatcggga
     2581 ggcgttggag atggttcacc gcccgacgag cgacgcgcaa tggcgccggg cccgcgaggc
     2641 cttgcgtcga atcgaggccc tcgtgctcca gacggcgttg ctcgagcggc gttcggagtc
     2701 ggaggcctcc tcatcggtgg cacggtctgc ggcgaagggc ggtcttcggg aaaagttcga
     2761 cgccaggctg ccgtacgcct tgaccgccga ccaaagcgcg gtgggggccg agatcgaggc
     2821 cgacctcgcc aggccccacc cgatgagtcg attgctgcag ggggacgtcg gcagcggcaa
     2881 gaccctcgtc gccgtgcggg cgatgctcgt cgcggccgag tcgggcgggc agtgcgccct
     2941 ggtggtgccg accgaggtgc tcgcggggca acatctgcgc tccatcgtgc gccttctcgg
     3001 ccccgacctt gccgccttgc tcgaacccac cctcgtgacg ggtgcccttt ccgcccgcga
     3061 gcgccgcacc gcgctcgtga ggatcgcctc cggccgggcg cggatcgtca tcggcacgca
     3121 cgcgttgttc ggtgacgccg tcgtcttcga cgacctggcg ctcgtcgtca tcgacgagca
     3181 gcaccgcttc ggcgtggagc agcgcgacgt gctgcgcacg aagggccgcc gggcgccgca
     3241 cacgctcgtt atgacggcga cgccgatacc gcgcaccatc gcgatgacgg ttttcggcga
     3301 tctcgacgtg tcgaccctgc gcacgatgcc gcccggccgg cggccggtac agacgatcgc
     3361 ggtgccgctc gccgagcatc cgcggtggga ggagcgcatc tgggcacgct cggccgaaga
     3421 ggtcgcagcc gggcggcagg tgttcctcgt ctgccccgcc atcgacgccg agccggcgcc
     3481 cgggggccgg cccgacgacg atgtgccgga cgatcccgac cacggtgccc cgccgcccgg
     3541 gcgcgccacg ctccaggccg gcatccccct cctgccggaa ggggaggagg cgccgggggt
     3601 cccccggtct tccgtcgagc aggtggcggc gacggccagg tctcgtgcgg aactcgccgg
     3661 cgcccgtatc ggggtcttgc atggccggct gccggccgag gagaagaact ccgtcatgga
     3721 ggccttcgcc gcgggcacga tcgacgtgct cgtgtcgacc acggtgatcg aggtcggggt
     3781 cgacgtgccg aacgcctctc tcatggtgat cttcgacgcc gaccggttcg gcctctcgca
     3841 gctgcaccag ctgcgcggcc gggtgggccg ggggggcgcc gctggcctgt gcctgctcgt
     3901 cacccgcgcg ctcgccgaga cgccggggcg cctgcgtgtc gacgccgtcg cggcgaacgc
     3961 cgacggattc gagcttgctc ggatagacct ggaactgcgc cgggagggcg acgtgctcgg
     4021 cgccgaccag tcgggccggc gctcctcgct gcggcttttg cgtgtgacgc gcgacggcga
     4081 cctgatcgcc gaggcgcgcg ccgacgcggc gcgcctgctc gcggcgccgg ggggtcttgc
     4141 ggctcacccc gcgctgggcg cggccctgca agaccggctc gacgacatcg agcgtgactt
     4201 tctggcgaga aactgacgac ggcatgcgtc aatccgcccg tgtcgcacgg cggaggccga
     4261 gggggccgag ggggccgagg gggccgggga agtgtgatca cgtcggcggg gacaaaaata
     4321 acggaaatat aacaatgaga taacgatgca ataacggccc tggtggctgg tagccggtag
     4381 tcttatctcg atatgaacag gatcgctgtc gtccctggat cgttcgaccc cgtcacgctc
     4441 gggcatctcg atgtcatcga gcgagccgcg aaactgttcg acgaggtgca cgtcgtcgtc
     4501 gtgcacaacc ccgacaaaga cgcgatgctg cccgtcgccc agcgggtctc cctcatcgag
     4561 cagtcgctcg acagcgctcg cctgggcgca acggtcctcg tcgcctcctg gagcatcggg
     4621 ctgcttgtcg actactgcag cgacgtgggc gcgcacgtgc tggtgaaggg catccgctca
     4681 caggtcgatg tggcctacga gacgccgatg gcgatcgtga accgcaacct ggccgacgtc
     4741 gagacggtct tcctgctgcc cgatcccgca cacgcgcacg tctcaagttc gctcgtgcgc
     4801 caggtcgcgg cgctgggcgg ggacgtgcgg ccgtacgttc ccacggccgt ctacgagtac
     4861 ctgcaggcga catgagcttg cgcggcgaca cctccgtcag caccctcggc cggccagcgg
     4921 cgccgtgggc gaaggacatc gccgcccgaa tcaccgccag cgtcgagacc gtcatccagg
     4981 gcaagcccga tgcgatccgc acagccctca ccgtgctgct ctcgggcggc catctgctga
     5041 tcgaagacgt gccgggcgtc ggcaagaccg tgctcgtcaa ggcgctcgcg cggtgcgtcg
     5101 gatgcagcgt cgcgaggatc cagttcacac ccgacctcct gcccggtgac gtcaccggtg
     5161 tcgccgtcta cgaccagcgc gaggcacgtt tcgtcttcaa acccggcccg gtcttcgccg
     5221 acatcgtgat cggcgacgag atcaaccggg ccagccccaa gacacagtcg gccctgctcg
     5281 aatgcatggc cgagcgtcaa gtcacggtgg acggcgccac gtacccgctc gcggttccct
     5341 tcacggtgtt cgccacgcag aacccgatcg agatggaggg aacctacgcg ctgccagagg
     5401 cgcaacggga tcgcttcacc gcgcgcatcg cgatgggcta tcccggccgg gtggcagagg
     5461 tcgcgatgct cggggcgaga gacggcgccg gcccgctcga gaggctcgcc ccggtcatcg
     5521 acgtcgagga tctgcgcgac gtgatggggc tcgtgcgccg ggtgtacgtc tcggcgccgg
     5581 tgaaacagta cgtcgtcgac atcgtgcgtg cgacgcgcga cgacccggag gtgcggatcg
     5641 gcgcgagccc gagggccacg ctgcagctgg tcgccgtcgc gaaggcgcag gccgtcatgg
     5701 acggccgcga cttcgcaatt cccgacgacg tggattccct ggccgtcgac gtgctcgccc
     5761 accgcctcat cctcctcgac gacgtgcggc ggccagacgc ccggcgcgcg gccggcgagg
     5821 tcgtcgggcg ggcgcttcgc cgggccgcgg ccgcgctgcc tgccgcaccg gggcccctgc
     5881 cgggtgcctt gcggcacggg aggtgaagcc acgtggccag gggacgacgg cccgcccgca
     5941 caccgcgggg catcctcgcc ctgaccggcc gaggcacggc tctcgctgtg gccggctttc
     6001 tcgttctcgc gctcgccgtg ctcgtggggc ggcaagatct actcgtcgtc ggggtgctgc
     6061 tcgtggcact gcccgcggcg gccctcgtct cgctgcgttt ggcaggtagc cttccgccgc
     6121 cgcagttgag cgtcgaccca ccggcggtcc ccgtgggcgg cacggccgtg ctcacgatcg
     6181 tttccggggc tgggtcggcg gccccggccg aggcgggcgc ctggggcaca atgcggcggc
     6241 tgtcgacagc gccgcaggcg gctccccggg ccgcaccgtg gcgattcgtc atgcccgacg
     6301 gcctggaaga cctcgggccg gccggtgccg cggcggacgg tgccggcggg ggtgggagga
     6361 tcaccgggcg agggacgcga cgcctcatcc gggccagccg tcgcggcgac tactcgatcg
     6421 gcccgctctt cctcgaggtc accgatcggt tcggcctcgc gcggcggcgc ctgtgcgccg
     6481 acggccgggg gcgcgtgttg gtgctccccg agcccgcgag gctctcctgg cccgtcacca
     6541 tgccgcccgg cgtgcgcgag aacgcctcac gcagcggacg aggtgtcggc ggcccgctgg
     6601 agggcaccac cgcgcggccc tatctgccgg gcgacccgat acgccgcatg cagtggcggg
     6661 cgacggcgcg attcggcaag ctgatggtgc gtcaggagga agaacggcga aggcccgagg
     6721 tgtggctgta cgtggatacc cgggggcggc cgggcacggc caagagggtg cgctctgcct
     6781 acgcctacga cgacgagtcc ttcgaactcc tggtgcgggc cgtcgcggcg gcggcgtcct
     6841 tctcggcggc gaggcgctgc gacctgcacg tcgtagaggc cggcgctcgc cagcttttcg
     6901 gcgagcggcc cggcggcggg cacgcctcct actccgcggc gcgggcacac acgctgctgc
     6961 cgcgagaact cgcgacgctg caccccgcgg cccgggggga gccggaggcc gccctcttcc
     7021 gcctgactgc ggcgctgcgg cgcccgggcg cgctcgtcgt cgcgttgcgg cccgaatgcg
     7081 acgacctcgt cggcgtgctc ggtgcggccc ggcccggggg cgccgccacg ggcactgtcg
     7141 tcgccgcggg ccccctcggc cccgccgtgc gggcgaggct cctcgccgcc ggatggcgga
     7201 tcgcagacgt cgcccggggt cgcgccgggc gaagggtcgc ctcatgaccg ccgcccgcga
     7261 gggcacggcc acgggcgccg ggcagtggcg caccgtcgcg gcgctcctct tcctcatcgc
     7321 cgcggccagc acctcgctcg gcggcctctt cgacgggccc gcgtggtggt tcctggccca
     7381 ggccgtcgcg gtggtggcgt tcaccgtggc ggcactgtcg cgcctggccg cgccggcctg
     7441 gtgcgcctcg cttggcgctg ccg
//
LOCUS       DASTNL010000091         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_992717, whole genome shotgun sequence.
ACCESSION   DASTNL010000091 DASTNL010000000
VERSION     DASTNL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992717"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFQ96_03050"
     CDS             <1..446
                     /locus_tag="VFQ96_03050"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0256797.1"
                     /translation="ALACDIRIAADTALFGQPEITLGNTPGWGGTQRLPRAIGAGRAT
                     SLMLTGRPIDAAAALAYGLVTEIVPPEALAGRAEALARDLAARSTHAVAAIKQAVRIG
                     AADFDAGLRAERLGVQTCCGTPEQVHAVQAFLGRHAAGTQKKGQR"
     gene            443..1951
                     /locus_tag="VFQ96_03055"
     CDS             443..1951
                     /locus_tag="VFQ96_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0256798.1"
                     /translation="MTLTKDSTTHAEPTRYGLVIDGQIVYGPKDDVLERRNPATGQVV
                     SIYARASRYQVDDAVAAARRAFDSGVWSDAAGMTRLRTLTALAGLMREHKGELARLDS
                     DEGGKPLRVALNDAEGAIGLMEFAASLAAADHGDIHTNLGGDFTGLLVREPIGVVGAI
                     VPWNFPLLILCQKVAFALAAGCTVVAKPSEFTSGSALLLAQLALEAGVPAGVYNVITG
                     EGATGQALAEHRDVDMITFTGSTATGRHVLEASKTNLKKTSLELGGKAAQIVFADADL
                     DAAVEGVVFGATHNQGECCVAGARLLVQAQIADEFDRRLAERAARLRVGGAEIDADYG
                     AMIHEGHLEKVLNAVEEAQAQGARVLTGGTRLTDPQHADGLFVAPTVLADVAPETDAF
                     QNEIFGPVLTVTKFDTAEEAVNLANGVAYGLANTIWTSSLDTALTVMRKLRSGTVYIN
                     TTIDGAPQMSFGGYKASGYGREMGRAGFEEFTQLKAVQIRTGNRVGTFGLRD"
     gene            2006..>2293
                     /locus_tag="VFQ96_03060"
     CDS             2006..>2293
                     /locus_tag="VFQ96_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0256799.1"
                     /translation="MAEDRPRPLDGVTVLDLTQILAGPYCTRLLADAGADVIKIEPPG
                     GDPSRGLPPVISPERSGYFANLNAGKRSVVADLRTPEGIALVRELATEADVV"
BASE COUNT          411 a          825 c          737 g          320 t
ORIGIN      
        1 tcgctttggc ctgcgacatt cgcatcgccg ccgacacggc gctcttcggc cagcctgaga
       61 tcacgctcgg caacacgccg ggctggggcg gcacacaacg cctgcctcgc gcgatcggcg
      121 ccggccgggc gacgtcgctc atgctgaccg gccggcccat cgacgctgcc gccgcgctcg
      181 cgtacggcct cgtcaccgag atcgtcccgc ctgaagcgtt ggccggccgg gcagaagccc
      241 tcgcgcgcga tctcgccgcc cgctcgacgc acgccgtggc cgccatcaaa caggcggtcc
      301 gtatcggcgc ggccgacttc gatgccgggc tgcgggccga acgcctcggt gtccaaacct
      361 gctgcggcac ccctgaacag gtgcacgccg tacaagcttt ccttggccgt catgcggccg
      421 gaacacaaaa gaaaggccag agatgacact cacaaaagac agcaccaccc acgccgaacc
      481 gacccgttat ggcctcgtca tcgacggcca gatcgtctac ggcccgaaag acgacgtcct
      541 cgagcggcgt aaccctgcga cggggcaggt cgtgtcgatc tacgcccgtg ccagccgcta
      601 tcaggtggac gacgcggtcg ccgcggcccg ccgtgcattc gactcggggg tgtggtccga
      661 cgccgccggc atgacgcgcc tgcgcacgct caccgccctg gcgggtctca tgagagagca
      721 caaaggagag ctggcacgcc tcgactccga cgagggcggc aaaccgctac gggtcgcgct
      781 gaacgacgcc gagggagcga tcggcctcat ggaattcgcc gcgagcctcg cggcagccga
      841 tcacggcgac atccacacca acctcggggg cgatttcacg ggcctcctcg tgcgggagcc
      901 gatcggcgtg gtcggggcga tcgtgccatg gaacttccca ttgctcatcc tgtgccagaa
      961 ggtcgccttc gcgctcgccg ccggttgcac cgtcgtcgcg aagccttccg agttcacctc
     1021 cggttccgcg ttgctcctgg ctcagctggc gttggaggca ggggtgccgg ccggcgtgta
     1081 caacgtcatc accggcgaag gggcgaccgg gcaagcgctc gccgaacacc gcgacgtcga
     1141 catgatcacc ttcaccggtt ccacggcgac gggtcgtcac gttctcgagg catcgaagac
     1201 gaacctcaag aaaacctcgc tggaactggg tggaaaggcc gcgcagatcg tcttcgccga
     1261 cgccgacctt gacgccgccg tggaaggcgt cgtcttcggc gcgacccaca accaaggcga
     1321 atgctgcgtc gcaggagcac gtctgctcgt ccaggctcag atcgccgacg aattcgaccg
     1381 gcggctggcc gaacgcgccg cacgcctgcg ggtgggcggc gccgagatag acgccgacta
     1441 cggcgcgatg atccacgagg ggcatctgga gaaggttctg aacgccgtcg aagaggcaca
     1501 ggcacaaggt gcgcgcgtcc tgaccggcgg cacgcgcttg accgacccgc aacacgccga
     1561 cggccttttt gtcgcaccca ccgtgctggc cgacgtggcg cccgagacgg atgcgttcca
     1621 gaacgagatc ttcggccccg tcctgaccgt cacgaagttc gacactgccg aagaggcggt
     1681 gaacctcgcc aacggcgtcg cctacggcct cgcaaacacg atctggacgt cgagcctcga
     1741 cacggcgctc accgtcatgc gcaagctgcg cagcggcacc gtctacatca acacgaccat
     1801 cgacggcgcg ccgcagatgt cgttcggcgg ctacaaggcc tccgggtacg ggcgagagat
     1861 gggtcgggcc ggcttcgaag aattcaccca gttgaaggcc gtgcagatcc gcaccggcaa
     1921 ccgggtcggc acgttcggcc tgcgggattg acgccatagc gacaccgcgc gacgcgacac
     1981 cgtgccagaa gagaagaaag aagcgatggc agaagacaga ccccgaccgc tcgacggcgt
     2041 gacggtcctc gacctcaccc agattctcgc cggcccgtac tgcacacgcc tgctggccga
     2101 cgccggggcc gacgtcatca agatcgagcc gcccggcggc gacccctccc gcggcctgcc
     2161 ccccgtcatc tcgccggaac gcagcgggta tttcgcgaac ctcaacgccg gcaagcgcag
     2221 cgtcgtcgcc gacctgcgca ccccggaagg catcgccctc gtgcgagaac tcgccaccga
     2281 ggccgacgtc gtg
//
LOCUS       DASTNL010000092         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_624785, whole genome shotgun sequence.
ACCESSION   DASTNL010000092 DASTNL010000000
VERSION     DASTNL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624785"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1107
                     /locus_tag="VFQ96_03065"
     CDS             109..1107
                     /locus_tag="VFQ96_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0256800.1"
                     /translation="MTEKSAEVTEGSGGLVGVAAAQFAPVADKQANIEHIGELTAQAR
                     GRGARLVVFPEYSAFFTPRLGPRMLAAAEAVDGPFVAGLATLADRLDVTIVAGLAEKS
                     PEAARFFSTVVAVAPRHGVVASYRKQHIYDAFGARESEWVRPGALGEPQTFSLGPFRV
                     GLQACYDVRFPEVTRTLLDAGADVVAVPAEWVPGPSKQEHWRTLLSARAIENTVFVIG
                     AGQTAPGGIGDSVILDPAGTALSELSTEPGLAVATLSPRLLSHVRETNPSLRLRRYAV
                     VPGRPGEAGSVREGADGRSEGGLAQFIPKGEGRHRHRIDDAQQGDGSFMHKEGAGR"
     gene            1104..>2197
                     /locus_tag="VFQ96_03070"
     CDS             1104..>2197
                     /locus_tag="VFQ96_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0256801.1"
                     /translation="MTDMPDETGNRPEKNASGVPGGAGSPTGPTQPYARPEGGMTPPP
                     APAAPEAGRDAFGRPYPGYPQPYGVGGQGGYENGYGAPADSAPAGRVKPLRRRNAGLI
                     VASLAVGALIGGAAGAAGGGLIGSYTASHGGQVSNVTTTGPANITVNDAKSATVVTAV
                     AAKASPSVVTINVTGAQESGTGSGIVLTKDGYVLTNTHVVTLDGDAANVQISVTDNNG
                     KIYPATIVGTDPVVDLAVIKLQGASDMAPATFANSEKVNVGQAAIAIGAPLGLSGTVT
                     DGIVSSINRSISISSSAAPKSSSGDSQDQQGGGGSGSENPFNFWNFTFPGQGGTDSGE
                     GGQQQQQQQTQASNQISLPVLQTDAAINPG"
BASE COUNT          378 a          743 c          775 g          301 t
ORIGIN      
        1 cgcggcacaa gaagagactc tagcacagcg tcccccaggg cctctgtggc ggtaccggga
       61 ggccgcgccg atgtgtcgcg gccggcgtcg ggccgggaga atagagacat gaccgagaag
      121 agcgccgagg tgaccgaagg ctcgggcggg cttgtcggcg tcgcggcggc acagttcgcg
      181 ccggtcgccg ataagcaggc taacatcgag cacatcgggg agttgaccgc gcaggcccgt
      241 ggcaggggag cccgactggt tgtcttcccc gaatactccg ccttcttcac gccccgcctc
      301 gggcctcgga tgctcgccgc ggccgaggcc gtcgacggcc cgttcgtggc ggggctcgcc
      361 accctggccg accgtctcga cgtcacgatc gtcgcggggc tcgcggagaa gagccccgag
      421 gcggcgcggt tcttcagcac ggtcgtggcg gtcgcacccc ggcacggtgt cgtcgcctcg
      481 tatcgcaaac agcacattta cgacgcgttc ggcgcccggg aatccgagtg ggtgcgtccc
      541 ggtgctctgg gtgagccgca gaccttttcg ttgggcccgt ttcgcgtcgg cctgcaggct
      601 tgctacgacg tgcgcttccc cgaggtgacg aggacgcttc tcgatgccgg cgccgacgtc
      661 gtcgccgtgc cggccgaatg ggtaccgggg ccttcgaagc aggagcattg gcgcaccctg
      721 ctctcggctc gggcgatcga gaacaccgtc ttcgtcatcg gagccggcca gacggcaccc
      781 ggcggcatcg gcgacagcgt catactcgac ccggcgggaa cggccctctc cgagctcagc
      841 acggagccgg gcttggccgt cgccacgctt tccccacgcc tcctgtcaca cgtcagggaa
      901 acgaacccga gcctacggct gcgtcgttat gcggtcgttc cggggcggcc cggcgaggcg
      961 gggtcggtgc gggagggtgc tgacggccgg tcggagggcg gactggcgca gttcataccg
     1021 aaaggtgagg gccggcatag acatcgcata gacgatgccc agcagggtga cggaagcttt
     1081 atgcacaagg aaggagcagg acgatgactg acatgcccga cgagaccggc aacaggcccg
     1141 agaagaacgc gagcggggtg ccgggcggcg caggttcgcc gaccgggccg acgcagccgt
     1201 acgcccgccc cgaggggggg atgacacctc cgccggcacc agccgcgccg gaagctggcc
     1261 gtgacgcatt cgggcggccc tacccaggct acccacagcc ctatggcgtc ggcggccagg
     1321 gcggctacga gaacggctac ggcgccccgg cagactcggc gccggccggc cgcgtcaagc
     1381 cgctgcgtcg gcgcaatgcg gggctcattg tggcctcgct cgccgtcggc gcgctgatcg
     1441 gcggggccgc aggggctgcg gggggcggcc tcattggttc ttacaccgcc tcgcacggcg
     1501 gccaggtgag caacgtcacg acgacggggc ctgcgaatat caccgtcaac gacgccaaga
     1561 gcgccaccgt ggtgacggcg gtagccgcga aggcatcacc cagcgtcgtg acgatcaacg
     1621 tcaccggcgc gcaggagagt ggcacggggt cggggatcgt cctcaccaaa gacggctatg
     1681 tcctcaccaa cacgcatgtc gtgacgctgg acggcgatgc cgcgaacgta cagatcagcg
     1741 tcaccgacaa caacggcaag atctacccgg cgacgatcgt cggcaccgac ccggtcgtgg
     1801 atctcgcggt catcaagctg cagggtgcca gcgacatggc gccggccact ttcgcgaact
     1861 cagagaaggt gaacgtcggc caggccgcga tcgcgatcgg cgcgccgctc gggctctccg
     1921 gcaccgtcac cgacggcatc gtcagctcga tcaaccggag catcagcatc tcctcgtcgg
     1981 cggcgccgaa gagcagcagc ggagactcgc aagaccagca gggtggcgga gggtccggct
     2041 cggagaaccc gttcaacttc tggaatttca ccttccccgg ccaagggggc accgacagcg
     2101 gcgaaggcgg gcagcagcaa cagcagcagc agacgcaggc ctcgaaccag atctccctgc
     2161 ccgtgttgca gactgatgcg gccatcaacc ccggcaa
//
LOCUS       DASTNL010000093         4245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_811686, whole genome shotgun sequence.
ACCESSION   DASTNL010000093 DASTNL010000000
VERSION     DASTNL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811686"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFQ96_03075"
     CDS             <1..799
                     /locus_tag="VFQ96_03075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEU0256802.1"
                     /translation="TLALLEAGARVAAVEIDGRLAGRLRTTVEERAAGAADRLTVVHQ
                     DAMTLTGPADVTGPGDGVASAAPTGGGPVQARGAAAAGEAARAGAPDRLVANLPYNVS
                     VPVLLRVLERFPSLASGVVMVQAEVGERLAASPGSKTYGAPSVKVAWYGVWRVRARIG
                     RGVFWPVPNVDSVLVGFERTPPPDGRAAAGAQEGGRGLLRERVFALVDAAFGQRRKML
                     RRTLAPLYGGAMAAARALTAAGIDPALRGERLTVGDFIRLAAGSEER"
     gene            796..3189
                     /locus_tag="VFQ96_03080"
     CDS             796..3189
                     /locus_tag="VFQ96_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU0256803.1"
                     /translation="MINRLRMVLRHNHTLRRLRFAPYALAYRIFTRFAQVRPGRVLFL
                     SDSRGGFSGNIAFLRDEILRQRPDTEVIGLFKAGLRELRVAHDMVRLPWLAATAQTIV
                     LDDYYPVIYPFRIRPETRLVQVWHAAGAFKRVGYSREGLPGGPVPGSIVHRNYTDVTV
                     SSEAVRANYAEAFGIDLARVAALGVPRTDAFFDEERIARATRAVRQRYGIPDGTKIVL
                     YAPTFRGNGQLSAHFDYESVDWEALTAGLGTGWSVLVKMHPFVRPLREARPWLRDVID
                     VSADREITELLMAADVLVTDYSSTIFEYGLLRRPIVFFCPDLEQYTADRSFYYPFEDY
                     VTGPLVRESSGLAAAIREARVDQKHADFLARQMGACDGHSSERVVRELILPPRAAGHP
                     AAVAPGSGPSPTPATGKMWLRVVVAYAARVLLNMAYAPMRILPRRRKVVLLSREHDTV
                     PADFTDLAAAIRRADPRVRVVTLVRMIPPGYAAKLGYAVHMLVQLYHVATARVLIIDT
                     YAMIASLLRHGDELTVVQVWHALGSFKKFGLSILGHAEGRDRRLAKAMRMHEGYDVVL
                     ASSAACRPALAEAFGTPEERIEVAPLPRVDRLRDPRRAAAIRQRITAAYPDLVGRRVA
                     VFAPTFRLDGAVSVDVGELAKALAAEGIHLVVKMHPLMSAEFGAGVDTAPGFSTQEML
                     QVADLFVTDYSSTLFEAAVVGVPCYFLAPDLDEYVSSRDFYLDYRHDLPGPVVRSIGG
                     LVTAVRAGGGTAAKARRFAERWVEVPETAGPSATVCADRVATLVVAALDRPALPGRP"
     gene            complement(3316..3774)
                     /locus_tag="VFQ96_03085"
     CDS             complement(3316..3774)
                     /locus_tag="VFQ96_03085"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF009084.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0256804.1"
                     /translation="MADNTQPARIAVVSARWHSDIVDRAVAGFEANIHDLLPTETEHF
                     TVPGAYEIPLHAQRLARTGRYDAIIACAFVVDGGIYRHEFVANTVVEALMRIQLETDV
                     PIFSGVLTPHNFHAHQEHIDYFTRHFEIKGKELAEAAAATLKSLRSLARV"
     gene            complement(3891..>4245)
                     /locus_tag="VFQ96_03090"
     CDS             complement(3891..>4245)
                     /locus_tag="VFQ96_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEU0256805.1"
                     /translation="DEQSLLGSLGSAGARYLEATLSEHEHLGISPWSSTLLATGAVGD
                     VCLRFFDADGRPTITELDERVLGIGRQTLLAVPRRIGIAGGERKHAAIRGALLGGWIN
                     ILITDKQTALALLKD"
BASE COUNT          569 a         1427 c         1542 g          707 t
ORIGIN      
        1 caccctcgcc cttctcgagg cgggagcgcg ggtcgccgcg gtcgagatcg acgggcggct
       61 ggccgggcgg cttcgcacga cggtcgagga acgtgcggcg ggggcggccg accggctcac
      121 cgtcgtgcac caagacgcga tgacgttgac cgggccggcc gatgtgacgg gccccggcga
      181 cggcgtggcg tcggcggcgc ctacgggggg agggccggtg caggcacgag gagcggcggc
      241 tgcgggggag gccgcgcgtg ccggcgcccc ggaccggctc gtcgcgaatc tgccctacaa
      301 cgtgtcggtg ccggtgcttc tgcgcgttct ggaacgattc ccctcgctgg cctccggtgt
      361 cgtcatggtg caggccgagg tgggggagcg cctggccgcc tcgccgggat cgaagaccta
      421 cggcgcaccc agcgtcaagg tcgcctggta cggcgtgtgg cgggttcgcg cgcgcatcgg
      481 gcgcggcgtc ttctggccgg tgccgaacgt cgactccgtg ctcgtgggct tcgagcgcac
      541 gcccccgcca gacggccggg cggctgccgg ggcgcaggaa ggtgggcggg gcctgctgcg
      601 tgagcgggtg tttgccctcg tcgacgccgc tttcggccag cgccgcaaga tgctgaggcg
      661 cacgctcgcg cccctttacg gcggtgcgat ggccgcggcg cgagccttga cggcagccgg
      721 catcgacccc gcgctacggg gggagcggct cacggtcggc gactttatcc gcctcgcggc
      781 ggggagcgag gaacgatgat caacaggctc cgcatggttt tgcggcacaa tcacacgctg
      841 agacgtctgc gcttcgcacc gtacgccctg gcctatcgga tcttcacgcg cttcgcgcag
      901 gtccgtccag gcagggtgtt gttcctctcc gactcgcgcg gcggcttctc gggcaacatc
      961 gcgttcttgc gggacgagat tcttcggcag cggccagaca ccgaggtcat aggcctcttc
     1021 aaggcggggc tgcgtgagct gcgcgtggcc catgacatgg tccgactgcc ctggctcgcc
     1081 gcgaccgccc agacgatcgt cttagacgac tattaccccg tcatctaccc gttccgtatc
     1141 cggccggaga ctcgtctcgt gcaggtgtgg cacgcggccg gggcgttcaa acgggtcggc
     1201 tacagccgcg aggggttgcc cggtggcccg gtgcccggat cgatcgtgca ccgcaactac
     1261 acggacgtga cggtgagcag cgaggcggta cgcgccaact acgccgaggc tttcggcatc
     1321 gacctcgccc gtgtggctgc gctcggggtg ccgcggaccg atgcgttctt cgacgaggaa
     1381 cgcatcgcgc gcgccacacg ggccgtgcgg caacgctacg ggattcctga cgggacgaaa
     1441 atcgttctct atgcgccgac gtttcgtggc aacggccagc tgagcgccca cttcgactac
     1501 gagagcgtcg actgggaggc gctcacggca ggcctcggca ccggctggtc ggtgctggtg
     1561 aagatgcatc cgttcgtgcg gccgctccgc gaggcgcggc cgtggctgcg cgacgtcatc
     1621 gacgtctccg ccgaccggga gatcacggag cttctcatgg cggcagacgt gctcgtcacc
     1681 gattactcct ccacgatctt cgaatacggc ttgctccgcc ggcccatcgt cttcttctgc
     1741 cccgacctcg agcaatacac cgccgatcgg tcgttctact accccttcga ggactacgtg
     1801 acgggtccgc tcgttcgcga gagctcgggc ctcgcggcgg cgatccgtga ggcgcgggtg
     1861 gaccagaagc atgcggactt cctcgcgcgg cagatgggag cctgcgacgg gcactcctcg
     1921 gagcgggtcg tgcgcgagct gattctcccg ccccgtgccg ccggccaccc ggccgcggtc
     1981 gctccgggct ccggccccag cccgacgccg gcgaccggca agatgtggct gcgtgtcgtc
     2041 gtcgcctatg ccgcccgcgt gctcttgaac atggcatatg cccccatgcg tatacttccc
     2101 aggcgacgca aggtggtgct gctgagccgt gagcacgaca cggtgcccgc cgatttcacc
     2161 gaccttgccg ccgcgattcg gcgcgccgac ccgcgcgtgc gggtcgtcac gctggtgcgc
     2221 atgattccgc cgggatacgc cgcgaagctc ggctatgcgg tgcatatgct cgtgcagctg
     2281 tatcacgtgg cgaccgcgcg ggtgctgatc atagacacct acgcgatgat cgccagcctt
     2341 ttgcgacacg gcgacgagtt gacggtcgtg caggtttggc acgccctggg ctcgttcaag
     2401 aagttcgggc tcagcatcct cggccacgca gagggccgcg accggcggct cgcgaaggcg
     2461 atgcggatgc acgaggggta cgacgtcgtc ctggccagca gcgccgcgtg ccggccggcc
     2521 ttggcggagg ccttcggcac gcccgaggag cggatcgagg tggcgccgct gccccgtgtc
     2581 gaccggctgc gcgaccctcg gcgtgccgcg gcgatcaggc agagaatcac ggcggcctat
     2641 cccgacctgg tggggcggcg ggtcgcggtg ttcgcgccca cgttccgcct cgacggcgcc
     2701 gtcagcgtgg atgtcggcga gctcgcgaag gccctcgccg cggagggcat ccacctcgtc
     2761 gtcaagatgc acccactcat gtcagccgag ttcggggccg gggtcgacac ggcgcccggc
     2821 ttctcgacgc aagagatgct gcaggtggcc gacctcttcg tgacggacta ttcgtcgacc
     2881 ttgttcgagg ccgccgtcgt cggcgtgcca tgctatttcc tcgcgcccga cctcgacgag
     2941 tatgtgtcgt cgcgagactt ctatctcgac tatcggcacg acctgccggg gccggtcgtt
     3001 cggagcatcg gtgggcttgt gacggctgtt cgcgcgggtg gcggcaccgc ggcgaaggct
     3061 cgccggttcg ccgaacgttg ggtggaggta cccgagacgg ccggtccgtc ggcaaccgtc
     3121 tgcgccgatc gcgtggcgac tctggtcgtc gcggctctcg accgccccgc cctgcctggc
     3181 cgcccgtaag cctcggccgg cgggcggcgg gggaagcctc ccggcgtcgc aggtcggccg
     3241 ccggcgggcg tggccgcctc tcggcggggg aagggagggg tggctggagg cgaagccggc
     3301 cgggtcttcg ggcggctaca cccgcgcgag cgatcgcagg cttttgagcg tggccgccgc
     3361 agcctcggcg agctccttgc ccttgatttc gaagtgccgg gtgaagtagt cgatgtgctc
     3421 ctggtgggcg tggaagttgt gcggggtgag cacccccgag aagatcggca cgtcggtctc
     3481 gagctggatg cgcatgagcg cctcgaccac cgtgttcgcg acgaactcgt ggcggtagat
     3541 cccgccgtcg acgacgaagg cacaggcgat gatggcgtcg taacggcccg tgcgcgccag
     3601 acgctgggca tgcagcggga tctcgtacgc gccgggcacc gtgaaatgtt ctgtctcggt
     3661 cggcagcagg tcgtgaatgt tcgcctcgaa gcccgccacc gcccgatcga cgatgtcgga
     3721 atgccaccgg gcggagacga ctgcgatccg agcgggttgt gtgttgtctg ccatctctgc
     3781 ctttcctcgg gtgtccgaca cgccgccgtg cgggccggcg ggcccggcaa cgcctcccgg
     3841 acgatcccgg ccacggtcgg gtgagcaggc tctcgtcgcc ccgccggact ctagtctttc
     3901 agcagggcga gggccgtctg cttgtcggtg atgagaatgt tgatccagcc gcccagcagg
     3961 gcgccgcgga ttgccgcgtg tttgcgttcc ccgccggcga tcccgatgcg gcggggaacg
     4021 gcgagcagcg tctggcggcc tatgccgagc acgcgctcgt cgagttccgt gatggtcggc
     4081 ctgccgtcgg cgtcgaagaa ccgaagacat acgtctccga ccgctccggt ggccagaagc
     4141 gtcgacgacc agggggagat gccgaggtgc tcgtgctcgc tgagggtcgc ctccagatag
     4201 cgggcgccgg ccgagccgag tgagcccagg agcgactgct cgtcg
//
LOCUS       DASTNL010000094         7704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1184123, whole genome shotgun sequence.
ACCESSION   DASTNL010000094 DASTNL010000000
VERSION     DASTNL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184123"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..714
                     /locus_tag="VFQ96_03095"
     CDS             277..714
                     /locus_tag="VFQ96_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEU0256806.1"
                     /translation="MSIADRTARTVWQGSIGAGSGSVTSGSGALGTLPVTWAARTEQP
                     GGKTSPEELLAAAHSSCFSMALAVTLGQKKLTPTSLDVQAKVTLDEVDGKPTITTSAI
                     TVVGTVPGVDAAGFQAIIDDAAKLCPVSRLFAGATITVEARLA"
     gene            complement(786..3512)
                     /gene="polA"
                     /locus_tag="VFQ96_03100"
     CDS             complement(786..3512)
                     /gene="polA"
                     /locus_tag="VFQ96_03100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU0256807.1"
                     /translation="MPHEKPTLLIIDGHSLAFRAFYALPLDSFVNDEGQHTNAIHGFV
                     AMLISLLKKEKPTHVGVAFDRSRHSFRTREYPAYKGTRGATPPEFLSQIPLLEKVLAA
                     MNIVTVSIDDYEADDIMATLARQGADEGYRVLVVSGDRDAIQLIRDDVTLLYPSVRGV
                     SALKRYDRDAVVERYGVEPGQYPEVAALVGETSDNLVGIAKVGDKTAVKWVQKYGTVD
                     EILAHADEITGVVGRNLREQKENALLNRRLNRLVDDVALPVGPSDFTRRAVDEAALRE
                     IFGVLKFRTLLDRVLDLDAPAEGTRAEEGGGAPRPEELRTDKDLAAWLQRVGAPGGAV
                     ARPSGGAPGGPATGAEATAPGGFAVVLTVEDAAASDDPARAVTLGAGIADETRCAWLA
                     DGGEALAAWLQGADPKTVFDAKAFYTSLDRAGVTPGGTVFDTKITGWLLDPAAPSKDL
                     AGQVRSLLGEELPGPAPEQLLPVPDPAGPGVEAWYLGRVASAIRERLTDGMLRVWEDI
                     ERPLTPVLARIEQDGVACDRGVLDALAGELDERAAELARQAYAEIGHEVNLGSPKQLQ
                     SVLFDELQMPKTRRNKSGYTTDAAALADLQQSHPHPFLDLLLRHRDATKLRQIVESLR
                     KAVGPDGRIHTTYDQVGTSTGRLSSADPNLQNIPARTPEGRRIRSAFVAGAGYETLLT
                     ADYSQIEMRIMAHLSGDDGLIEAFRQGEDLHRYVGSHVFGVAPEDVTAQMRTKVKAMS
                     YGLVYGLSAYGLSRQLHIETAEARRLMAEYFERFGAVRDYLRNVVEQARVDGFTQTIF
                     GRRRFFPDLSSTNRVRRDNAERAALNAPIQGSAADIIKIAMVAIARDMTRQNMGSRML
                     LQVHDELIFEVAPGEWEALETIVRGRMAGAAELTVPLVVQVGRGADWNSAAH"
     gene            complement(3583..4191)
                     /locus_tag="VFQ96_03105"
     CDS             complement(3583..4191)
                     /locus_tag="VFQ96_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0256808.1"
                     /translation="MNDQEPSASAPRRVVVAEDESLIRLDIVEILRDNGFEVVGEAGD
                     GETALALATELRPDLVVMDVRMPKMDGIAAAKKLSEDHVAPVVLLTAFSQKDLVERAT
                     EAGALAYVVKPFTPNDLLPAIEIALARHAQIIALEAEVSDLVERFETRKLVDRAKGLL
                     NEKMGLTEPEAFRWIQKASMDRRLTMHDVSLAIIDQLGAKKK"
     gene            4229..4311
                     /locus_tag="VFQ96_03110"
     tRNA            4229..4311
                     /locus_tag="VFQ96_03110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4262..4264,aa:Leu,seq:caa)
     gene            complement(4339..5790)
                     /gene="pyk"
                     /locus_tag="VFQ96_03115"
     CDS             complement(4339..5790)
                     /gene="pyk"
                     /locus_tag="VFQ96_03115"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0256809.1"
                     /translation="MRRAKIVATLGPATSDYENIRAIIDAGVDVARMNLSHGTYAIHE
                     GVYANVRRAAADAGRAIAVMVDLQGPKIRLGKFEGGPYQLEVGDTFRITTDDVLGTKE
                     LSSTTFKGLPHDVKPGDFLLIDDGKVKVEVTGVDGPVVATRVVVAGAVSNNKGINLPG
                     VAVSVPALSEKDEADLRWGLRLGADLIALSFVRGAADIERVHEIMAEEGRTLPVIAKI
                     EKPQAVDALEEIVAAFDAIMVARGDLGVELPLEAVPIVQKRAVELARRMAKPVIVATQ
                     MLESMIGSPVPTRAETSDVANAVLDGADAVMLSGETSVGKYPVVTVRTMARIVESTEE
                     HGLDRIPPLGTTPHTQSGALTLAAAEVADFVGARFLCVFTQSGDSAHRVARLRCGIPM
                     LAFTPLPEIQRQLSLCWGIRSYLVDMVTHTDQMFAQVDDIVLAGGLAEVGDKVVVISG
                     SPPGIAGSTNDVRIHVVGDAQNGAAPAYSALRP"
     gene            complement(5887..6879)
                     /gene="lgt"
                     /locus_tag="VFQ96_03120"
     CDS             complement(5887..6879)
                     /gene="lgt"
                     /locus_tag="VFQ96_03120"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU0256810.1"
                     /translation="MTVLSMLPLSIPSPPLSWASFTIPLFGLHLTVRTYALCILAGII
                     VAAAWSSRRLSKRGAEPGVILDIVLWAVPLGIIVARFWHVFTHPHDYFYPGANLLNVF
                     YIWDGGNAIFGAILGGAVGAFIACRIAGLRFWVFADALAPAMLVAQAIGRLGNYANNE
                     LFGLPTNLPWGLQISSGNPAFPAGLPSGTLFQPLFLYEIIWDVLGAVVIVAIERRHAM
                     HWGIGIGFYGLWYGAGRSFLEMIRIDPSEYSFLSIPFNAWAAILAAVLGIVIMAVQHR
                     RHPGAEPGAYLPGREWIGKHTLPDSKQNDWEEGTEPLGPLPIADDDQQHITSGA"
     gene            complement(7063..>7704)
                     /gene="trpA"
                     /locus_tag="VFQ96_03125"
     CDS             complement(7063..>7704)
                     /gene="trpA"
                     /locus_tag="VFQ96_03125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU0256811.1"
                     /translation="ELGIPYSDPVMDGPVIQHATHRALEAGFRVRDTFTAIRRITQQV
                     PIPVIVMTYWNPVLRFGPQRFTEELLESGGTGLITPDLIPDEAGEWLSVSGAAGAERV
                     FLAAPTSTDERLRRIADLSRGFVYAVSTMGITGERRDVDQAARTLVGRLRGFDTPRVC
                     VGIGISTGDQVREVLGYADGAIVGSALIAALDGSGVAGAAAKAAELSAGKSGG"
BASE COUNT         1188 a         2619 c         2645 g         1252 t
ORIGIN      
        1 ggcctgcttc tcggtcgttt cggttgtcat agaagtgtgt gctccagggt ggacatgaat
       61 cgggcccggc tcggaggcgt catcggccag caacagcggc cgcaccgcat ccgcgctggg
      121 cgaacggatg aaaacgtcac aagtgtgaca tcccatgcta tcggaccagc ctggggcagt
      181 ccaccgcacc ggtcgatcgg ctggcctcgc gcaggccaca cacgatagtt ttgaggagaa
      241 ccctcacgtt aaatgatcga gagagaggat gccactatga gcatcgcgga tcgcacggca
      301 cgaacggtat ggcagggcag catcggcgcg gggtccggct ccgtgacctc gggtagcggc
      361 gccctcggaa cactgcccgt gacctgggcg gcgcgcaccg agcagcccgg cggcaagacc
      421 agtcccgaag agctcctggc cgcggcgcat tcctcgtgct tctccatggc actcgccgtg
      481 acgctcgggc agaagaagct cacgccgacc agcctcgacg tccaggcgaa ggtcacgctc
      541 gacgaggtcg acggcaagcc gacgataacc acctcggcga tcacggtcgt aggcaccgtg
      601 cccggggtgg acgccgccgg cttccaggcc atcatcgacg acgcggcgaa gctctgcccg
      661 gtctcgcgtc ttttcgccgg cgcgacgatc accgtcgagg cgcggctggc ctgaggcctc
      721 ccccgttgca aaggtgggtg tcgcccggcc gggcgacacc caccttcgtt tgacgcctgc
      781 ctcgactaat gggccgcgga gttccagtcg gccccgcgcc cgacctgcac gacaagcggc
      841 acggtgagct ctgcggcacc ggccatccgc ccgcgcacga tcgtctccag cgcctcccac
      901 tcccccggtg cgacctcgaa gatcaactcg tcgtgcacct ggagcagcat gcgtgagccc
      961 atgttttgcc gcgtcatgtc gcgggcgatg gcgaccatgg cgatcttgat gatgtcggcc
     1021 gccgaaccct ggatgggcgc gttcaaggca gcccgttccg cgttgtcgcg gcgtacccgg
     1081 ttcgtgctgg aaaggtcggg gaagaacctt cggcggccga agatcgtctg ggtgaagcca
     1141 tcgacgcggg cctgctcgac gacgtttctc aggtagtcgc gcacggcgcc gaaacgctcg
     1201 aagtactccg ccatgaggcg cctggcctcc gccgtctcga tgtgcaactg gcgcgaaagg
     1261 ccgtaggcgc tcaggccgta caccagcccg taggacatcg ccttcacctt ggtgcgcatc
     1321 tgcgcggtga cgtcttcggg cgccacgccg aagacgtgtg agccgacgta acggtgcagg
     1381 tcttcgcctt gccgaaaggc ttcgatgaga ccgtcgtcgc cgctcaggtg cgccatgatg
     1441 cgcatctcga tctgcgaata gtcggcggtg agaagggtct cgtaccctgc cccagcgacg
     1501 aacgccgagc ggatgcgccg gccctccggg gttctcgcgg ggatgttctg caggttgggg
     1561 tcggcggagg agagcctgcc ggtcgacgtg ccgacctggt cgtaggtggt gtgaatgcgg
     1621 ccgtcgggcc ccacggcctt ccgcaacgac tcgacgatct gccgcagctt ggtggcgtct
     1681 cggtggcgta gcagcaagtc gaggaacggg tgcggatgcg actgctgcag gtcggccagc
     1741 gccgccgcgt cggtcgtgta gccggatttg ttgcgtctgg tcttcggcat ctgcagctcg
     1801 tcgaagagca cgctttgcag ctgcttcggc gagcccaggt tgacctcgtg cccgatctcg
     1861 gcataggcct ggcgggcaag ctccgcggcg cgctcgtcga gctcacccgc cagagcgtcg
     1921 agcacgccgc ggtcacaggc cacgccgtct tgctcgatcc gtgccaagac cggcgtcagg
     1981 gggcgttcga tgtcttccca gacccggagc atgccgtcgg tgagacgttc ccggatcgcc
     2041 gacgcgacac gacccaggta ccaggcctcc actccgggcc ctgccgggtc gggcaccggc
     2101 agcagctgct cgggtgccgg cccgggcagc tcttccccca ggagagagcg cacctggccg
     2161 gccaggtcct tgctcggcgc ggctgggtcg aggagccagc cggtgatctt cgtgtcgaac
     2221 acggtgccgc cgggagtgac gcccgcccgg tcgaggctcg tgtagaaggc tttggcgtcg
     2281 aagacggtct tcgggtcggc gccctgcaac caggcggcga gggcttcgcc gccgtcggcc
     2341 aaccaggcgc agcgcgtctc gtcggcaatc cccgcgccca gggtgacggc gcgagccgga
     2401 tcgtccgacg ccgcggcgtc ctccacggtg aggaccacgg cgaacccgcc cggggccgtc
     2461 gcctcggcgc cggtggccgg gccgcccggg gccccacccg acggccgtgc cacggccccg
     2521 ccgggcgcgc ccacccgctg cagccacgcg gcgagatcct tgtccgtgcg aagctcctcg
     2581 ggccggggcg cgccgccgcc ttcttcggcc cgggtgcctt ccgccggcgc atcgaggtcg
     2641 aggacccggt cgagcagcgt gcggaacttc aagactccga agatctcccg cagcgccgcc
     2701 tcgtcgacgg cgcgtcgcgt gaagtccgac gggccgaccg gcaacgcgac gtcgtcgacc
     2761 aggcggttca ggcgccggtt gagaagcgcg ttctccttct gctcgcgcaa attccggccc
     2821 acgacccccg tgatctcgtc ggcgtgcgcg aggatctcgt ctaccgtgcc atacttctgc
     2881 acccatttca cggcggtctt atcgccgacc ttcgcgatgc cgaccaggtt gtcgctggtc
     2941 tcgccgacga gcgcggcgac ctcggggtac tgcccgggct cgacgccgta tcgctcgacg
     3001 acggcatcgc ggtcgtaacg cttcaaggcc gagacgccgc gcacgctggg gtagagaagg
     3061 gtgacgtcgt cgcggatcag ttggatcgcg tcgcggtcgc ccgagacgac gagtacgcgg
     3121 tagccctcat cggccccttg ccgggcaagc gtcgccatga tgtcgtccgc ctcgtaatcg
     3181 tcgatgctga cggtgacgat gttcatcgcc gcgagcacct tctcgaggag cgggatctgg
     3241 ctcaggaact cgggcggcgt cgcgccgcgc gtgcccttgt aggcgggata ctcacgggtg
     3301 cggaacgagt gccgcgaacg gtcgaacgcc acgccgacgt gggtcggctt ctccttcttc
     3361 agaaggctta tgagcatggc gacgaagccg tggatcgcgt tcgtgtgctg gccctcgtcg
     3421 ttgacgaagc tgtcgagcgg caacgcatag aaggcgcgga acgcgagcga atgcccgtcg
     3481 atgatgagca gggtcggctt ttcgtgcggc accgcaccag cttacaagcg ggcctcgcag
     3541 cgcggcccgt ccggccggcg gggctgcccg aaggcgcccc cgctacttct ttttcgcgcc
     3601 cagctggtcg atgatcgcca acgacacgtc gtgcatcgtg agcctgcggt ccatcgaagc
     3661 cttctggatc caccggaatg cctcgggctc ggtgaggccc atcttctcgt tgaggaggcc
     3721 tttcgcgcgg tcgacgagct tgcgggtctc gaaccgctcg accaggtcgg agacctctgc
     3781 ctcaagggcg atgatctgcg cgtggcgcgc gagcgcgatc tcgatggccg gcagaagatc
     3841 gttcggcgtg aacggcttca cgacgtaggc cagtgccccg gcttcggtcg cccgctcgac
     3901 gaggtccttc tggctgaacg ccgtcagcag cacgaccggc gcgacgtggt cttcgctgag
     3961 tttcttcgcc gcggcgatgc cgtccatctt cggcatgcgc acgtccatga cgaccaggtc
     4021 gggccgcaac tcggtggcca aggccagcgc cgtctcaccg tcgcccgcct cgccgacgac
     4081 ctcgaacccg ttgtctcgca gaatctcgac gatatcgagt ctgatcagcg attcatcttc
     4141 tgccacgacg acgcgtcgcg gcgcggacgc ggacggttct tggtcattca cgggtcaagc
     4201 ttacggtatt ctggatgccg tcgtgccggc cggtgtggcg gaatggtaga cgcggagcac
     4261 tcaaaatgct ttgcccgaaa gggcgtgtgg gttcgactcc caccaccggc acgcggcccg
     4321 gcagggcacc ccgccgactc acggtcggag ggcgctgtag gccggcgcgg cgccgttctg
     4381 cgcatccccc accacgtgga tgcgcacgtc gttcgtcgac ccggcgatcc cgggcgggga
     4441 gccggagatg acgacgacct tgtcgccgac ttctgcgagg cccccggcca gcacgatgtc
     4501 gtctacctgc gcgaacatct ggtcggtgtg cgtcaccatg tcgacgagat atgaccggat
     4561 cccccagcac agcgagagct ggcgttgaat ctcgggcagc ggcgtgaacg ccagcatcgg
     4621 gatgccgcag cgcagcctgg cgacgcggtg tgcagagtcg cccgactggg tgaagacgca
     4681 gaggaagcgg gcgccgacga agtctgcgac ctcggccgca gcgagcgtca acgcaccgct
     4741 ctgggtgtgc ggggtcgtgc cgagcggcgg gatgcggtcg aggccgtgct cttcggtcga
     4801 ctccacgatg cgcgccatgg tgcgcacggt gacgaccggg tacttgccga cgctcgtctc
     4861 gcccgagagc atcaccgcgt cggcgccgtc gagcaccgcg ttggcgacgt cggaggtctc
     4921 ggcgcgggtc ggcacggggc tgccgatcat cgactcgagc atctgggtcg cgacgatgac
     4981 cggcttcgcc atgcgcctgg caagctccac cgcgcgcttc tgcacgatcg gcaccgcctc
     5041 cagcggcagc tccacgccca ggtcgcctcg ggcgaccatg atcgcgtcga acgcggcgac
     5101 gatctcttcg agcgcgtcga ccgcctgcgg tttctcgatc ttcgcgatga cgggcagggt
     5161 gcggccttct tcagccatga tctcgtgtac ccgttcgatg tccgcggcgc cgcggacgaa
     5221 cgagagggcg atgaggtcgg cgccgagccg caggccccat cgaaggtcgg cctcgtcttt
     5281 ctcgctcaag gcgggcacac tgacggcgac acccggcagg ttgatgcctt tgttgttcga
     5341 gaccgcgcct gcgacgacga cgcgcgtcgc gacgaccggc ccgtcgacgc ccgtgacctc
     5401 gaccttgacc ttgccgtcgt cgatcagcag aaagtcgccc ggcttcacgt cgtgcggcag
     5461 acccttgaac gtcgtcgacg agagctcctt cgtgcccagc acgtcgtcgg tcgtgatgcg
     5521 gaacgtgtcg ccgacctcga gctggtaggg tccgccctcg aacttgccga gcctgatctt
     5581 cgggccctgc aggtcgacca tgaccgcgat cgcccggccc gcgtcggccg ccgcccggcg
     5641 cacattcgcg tagacccctt catggatcgc ataggtgccg tgactgaggt tcatccgcgc
     5701 cacgtcgacg cccgcgtcga tgatcgcacg gatgttctca tagtctgatg tcgccgggcc
     5761 gagggtcgcg acgatcttcg cgcgtctcat tgtctgtgtg ttctcctgca tatacgtcgt
     5821 ggcgaagcgc gatgatgtgt gtcgttgtgg ccccgggaag ccggcctgtg ggcggcccgg
     5881 gtgtggtcag gcgccgctcg tgatgtgttg ctggtcgtcg tctgcgatag ggagcgggcc
     5941 gagcggctcg gtgccttctt cccaatcgtt ctgtttcgag tcgggcagcg tgtgcttgcc
     6001 gatccactcc cggccgggca ggtaggcccc gggctccgca ccggggtggc gccggtgctg
     6061 cacagccatg atgacgatgc ccagcaccgc cgcgaggatg gccgcccagg cgttgaaggg
     6121 gatcgagagg aagctgtact cgctcgggtc gatgcggatc atctcgagga aggagcgccc
     6181 ggcgccatac cacaggccgt agaagccgat gccgatcccc cagtgcatcg cgtgacggcg
     6241 ctcgatggcg acgatgacga cggcgcccaa gacatcccag atgatctcgt acaggaacag
     6301 cggctggaag agcgtgccgg agggcagccc ggccgggaaa gccgggttgc cagacgagat
     6361 ctgaagaccc cacggcaggt tcgtcggcag gccgaaaagc tcgttgttcg catagttgcc
     6421 gagccgcccg atcgcctggg ccaccagcat ggccggcgcg agggcgtcgg cgaaaaccca
     6481 gaaccgcagg cccgcgatgc ggcaggcgat gaaagcaccc accgcgccgc ccagtatcgc
     6541 accgaagatg gcgttgccgc cgtcccagat gtagaagacg ttcagcaggt tcgcgcccgg
     6601 atagaagtag tcgtgcggat gcgtgaagac gtgccagaag cgggcgacga tgatgcccag
     6661 cggcacggcc cacaagacga tgtcgaggat gacgcccggc tcggcgcccc gcttcgacag
     6721 ccggcgggac gaccaggcgg ccgcgacgat gatgccggcc aggatgcaca acgcgtaggt
     6781 gcgcacggtc aggtgcagcc cgaagagggg gatggtgaac gacgcccagg acagcggcgg
     6841 actcgggatg ctcaggggca gcatgctcag gacggtcacc cggtgactca cctctctcga
     6901 ttgttcgtct cggcggcgta cggcgccccc gcgcgtcggt cacgggggcc gcggccaccc
     6961 tagcctatcg acttatccga ggctcccgga cgccgcttgg gcggccccga cgggccttgc
     7021 cgggcccctg tggaggaggc ccggcggggc gcctcgcgcc cgtcagccgc ccgacttgcc
     7081 ggccgaaagc tcggccgcct tggcggccgc gccggcgaca cccgagccgt ccagcgccgc
     7141 gatcagagcg gaaccgacga tcgcgccgtc tgcatagccc agcacctcgc gtacctggtc
     7201 gcccgtcgag atgccgatgc ccacgcagac gcgtggcgta tcgaaccctc gaaggcgccc
     7261 gaccagcgtg cgcgccgcct ggtccacgtc gcggcgttcg cccgtgatgc ccatggtcga
     7321 gaccgcatag acgaaaccac gcgagaggtc cgctatgcga cgcagcctct cgtcggtgga
     7381 ggtgggtgcc gcgaggaaaa cccgttccgc acccgccgcc ccggatacgg agagccattc
     7441 gccggcctcg tcgggaatga ggtcgggcgt gatgaggccc gtgccgccgc tttcgagaag
     7501 ctcctcggtg aagcgttgcg ggccgaaacg cagcaccggg ttccagtagg tcatgacgat
     7561 caccggtatc ggcacctgct gcgttatgcg ccgtatcgcc gtgaacgtgt cgcgcacccg
     7621 gaacccggcc tccaacgcac ggtgcgtcgc gtgctggatc acggggccgt ccatgaccgg
     7681 gtctgaatac gggatgccga gctc
//
LOCUS       DASTNL010000095         7397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_629216, whole genome shotgun sequence.
ACCESSION   DASTNL010000095 DASTNL010000000
VERSION     DASTNL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629216"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFQ96_03130"
     CDS             complement(<1..1036)
                     /locus_tag="VFQ96_03130"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU0256812.1"
                     /translation="MSAGEAAEASRHEGAPVEGFDAVDLIHAKRDGRELSGDQIAWLI
                     DAYTRGYVGDEQMAAMAMAVFLNGMTRRETHDLTRSMIASGERLDFGDLGKATVDKHS
                     TGGVGDKITLPLMPLVASFGVAVPQLSGRGLGHTGGTLDKLESIPGWRAELSLAEMRA
                     QLRGPGGVVCAAGAGLAPADKKLYALRDVTGTVESIPLIASSIMSKKIAEGTGALVLD
                     VKFGSGSFMGDIERSRELAQAMVELGHDAGVRTSALLTNMNVPLGRAIGNANEVRESV
                     EVLAGGGPADVVELTLALAREMLALVGLPDADVAGALAGGRAMDVWRATVRAQGGDPD
                     AALPLPRHTHT"
     gene            complement(1033..1455)
                     /locus_tag="VFQ96_03135"
     CDS             complement(1033..1455)
                     /locus_tag="VFQ96_03135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU0256813.1"
                     /translation="MTGKKPSEIDWGALRRRASEALEHAYAPYSRFPVGAAALVTDGR
                     VVAGCNVENASYGLSLCAECGLVSELHATGGGRLVAFSCVDGRGQTLMPCGRCRQLLF
                     EHAAPGMLLETVSGIRTIDEVLPDAFGPGDLTRAEAAA"
     gene            2062..3162
                     /locus_tag="VFQ96_03140"
     CDS             2062..3162
                     /locus_tag="VFQ96_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0256814.1"
                     /translation="MSHRMKLAVSALVAGAALVLAGCSAPPPSASPSSSSSSGSKTAS
                     SKFMPCVVSDTAGFNDHSFNQLSLQAVQEAAKKAGTTYKMAKSQTASDYVPNVNQLVA
                     AGCTATVASGFNLVAAIKGAAKANTNKDFVMVDDNSINLPNVKDVVFATNQAAFLGGY
                     AAAAYSKTGVVATWGGQEIPPVTIYMDGFWDGVQYYNQQNHKNVKVLGWNEKTQKGTF
                     VGNFTDQNTAKTDTTNFINQKADVIMPVAGPLYEGAGAAIRATKANAVLVGVDADLFK
                     TDTSGYKDLILLSVMKGIQPAVATVLDQSAAGQFSATQYIGTLKNNGVAISPFHDFTS
                     KVPKNLSAQLAKIKAGIIDGSIQAPSPAEPKG"
     gene            3242..4762
                     /locus_tag="VFQ96_03145"
     CDS             3242..4762
                     /locus_tag="VFQ96_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256815.1"
                     /translation="MRLELRGITKRFGSLVANDHIDLAIEPGEVHCILGENGAGKTTL
                     MNMLYGLLPIDEGEIVIDGAAQHFQSPRDAMHAGIGMVHQHFMLVPVFTVAENVMLGH
                     EETTFAGRLNLAAARDTVRRIADQFGFDLDPDARIEDLPVGVQQRVEIIKALALDAQV
                     LVFDEPTAVLTPQETDELMAIMRRLKESGRAIVFITHKLREVREVADRITVMRLGKIV
                     GTVHPGASDEEMASLMVGRPVELTVQKGEAAPGEPALVVTGLTMREGGARPVLDNVTF
                     TVHRGEILAIAGVQGNGQTELVEALFGMQPEATGRATIDGRNLLGVGARAAIESGVGF
                     VPEDRQEDGLVGAFTIAENLMLNGSSAAPFVRHGNLQLRYLRRFADERIREFDVRTQS
                     AATLARQLSGGNQQKVVLARELSRDLRLLIAAQPTRGLDVGSIEFVHERIVQARDEGV
                     PVVVVSTELDEVVALADRIAVMYRGTIVGIVPPTTPRETLGLMMAGQMPDGVEKAA"
     gene            4759..6087
                     /locus_tag="VFQ96_03150"
     CDS             4759..6087
                     /locus_tag="VFQ96_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256816.1"
                     /translation="MSIEQQDPSPAGRVAGTDEAPDTPAALPHGAAAAAAGESTPEPE
                     SRSARFLREVTSGSGMITVLAILVGLVAGAVLIVVTNSSVLAAMQYFAAAPGATFRAV
                     GDTVGGAYAALFRGGIYDYTQTSFIAGLQPLTQSMSFAVPLIAAGLGIGIGFRAGVFN
                     IGGQGQMLAGGAFAGWIAYALPMPAALHIPVAILGALVGGAICAGIAGLLKATTGAHE
                     VIVTIMLNYVALYMVGYLLTTALKAPGSNNPISPAALPSAVLPGKTAGLNLGFVLAIA
                     LTLAAAWLMNRSALGFQFRAVGENPRAARVAGISVKKVVILTMIISGAFVGFAGAYEV
                     LGQTTSGFTNVLDAGVGFNAITVALLGRNKPWGIFWAGILFGVFQAGGYAMQASQGVD
                     IDVIQVVQSAIVLFIAAPPLVRAIFRLPEAGGRTKRTRSPKRTTKEAVAR"
     gene            6084..7367
                     /locus_tag="VFQ96_03155"
     CDS             6084..7367
                     /locus_tag="VFQ96_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256817.1"
                     /translation="MSTLATTAAPAGTGEAPLRLRDWKAPILFGVFTLFAVILFFAFG
                     KGGVSTFTFTAPATDVIHVPEIHIAARPVGIVAALVAVAMTVIDARWVRTRRKTPLWF
                     TTLFAVILVVAFLAWSTVGHSLPIVGLLGGAIALSVPLIFGALGGVVSERAGVVNIVI
                     EGQLLAGAFVSAVAASMTHSATIGLIAAAVAGMLVSFMLAAFSITYFVDQIIVGVVLN
                     VLVLGLTNFIYQTSLTQNPQLLNSPKQLPTLPIPGLSAIPIVGPVLFNQTIIVYLMYG
                     VIAAVFYVLFFTGVGLRIRAVGEHPAAADTVGINVNSTRFWSVSLGGAIAGLGGAFFT
                     LGSVGAFTEDVSSGEGYIALAAVIFGGWHPIKAMFAALLFGLANELQSILGILGSPVP
                     SQFLLMVPYVVTIIAVAGLVGQSRAPAADGKPYFK"
BASE COUNT         1150 a         2645 c         2453 g         1149 t
ORIGIN      
        1 ccgtgtgcgt gtgccggggc agcggcaggg ccgcgtcggg gtcgccgccc tgggcgcgca
       61 ccgtggcccg ccagacgtcc atcgcacggc cgccggcgag agcgcccgcc acgtcggcgt
      121 cgggcaagcc gacgagcgcc agcatctccc gggcgagcgc cagcgtcagc tcgacgacat
      181 ccgccggccc gccgcccgcc agcacctcga ccgactcccg cacctcgttg gcgttgccga
      241 tggcgcggcc gagcggcacg ttcatgttgg tgaggagggc gctggtgcgc acccccgcgt
      301 cgtgccccag ctcgaccatc gcctgcgcca gttcgcgaga acgctcgatg tcccccatga
      361 aggagccgga gccgaacttc acgtcgagca cgagcgcacc cgtgccctcc gcgatcttct
      421 tcgacatgat ggaagaggcg atgagcggta tcgactcgac cgtgccggtc acgtcgcgca
      481 gcgcgtagag cttcttgtcg gcgggcgcga ggccggcgcc ggccgcgcag acgacgccgc
      541 ccggcccgcg cagctgcgca cgcatctcgg cgaggctgag ctccgcccgc cagccgggta
      601 tcgactcgag cttgtcgagg gtgccgcccg tgtggccgag gccgcggccg gagagctgcg
      661 gcacggcgac gccgaacgag gccacgaggg gcatgagggg cagcgtgatc ttgtctccca
      721 ccccgccggt ggagtgtttg tcgaccgtgg ccttgccgag gtcgccgaaa tcgagccgct
      781 cgccactggc gatcatcgaa cgcgtgaggt cgtgcgtctc gcgccgggtc atgccgttca
      841 agaagaccgc catcgccatg gccgccatct gctcgtcgcc cacgtagcca cgcgtgtagg
      901 cgtcgatgag ccaggcgatc tggtcgccgg agagctcccg gccgtcgcgc ttggcatgga
      961 tgaggtcgac ggcgtcgaac ccttcgaccg gcgcgccctc gtgacggctc gcttcggccg
     1021 cctcgcccgc gctcacgccg ccgcctccgc ccgcgtgaga tcccccgggc cgaacgcgtc
     1081 gggcagcacc tcgtcgatgg tccggatgcc ggagacggtc tctagaagca tgcctggggc
     1141 ggcatgttcg aagagcagct gccgacaccg gccgcacggc atgagcgtct gcccgcggcc
     1201 gtcgacgcag ctgaacgcga cgagccgccc gccgccggtg gcgtgcagct cggagaccag
     1261 accgcattcg gcgcacaggc tcagcccgta gctggcgttc tccacgttgc agcccgcgac
     1321 gacgcgcccg tcggtgacga gcgcggccgc gccgacggga aagcgggaat acggcgcgta
     1381 ggcgtgctcc aacgcctcgc tcgcccggcg gcgcaaggcg ccccaatcga tctctgacgg
     1441 cttttttccg gtcatggtcg gtacggcttt cccttcgcgc ggggccccgg ccgcccgacc
     1501 ggccccggcg cacgaagatc gtgacgacgc ccggcggcgg ctgcgggcgg agccgtggcg
     1561 gccccgggcg cgccggcgat cagacgtggt tgtgctgtcg ggcagcacca tgcagcatac
     1621 ggctcttacc gccactcagg caccaacaaa tgagcaaaaa cccacggttg cgcagcctga
     1681 agagggcagt gtaaacctga acggtatccg tcgacgcgcg tagacatcgc gcgtcttccc
     1741 gaggagcctg tcgatgagga gggccggcat gccaccgtgc gaagcaagcc gccagaaggc
     1801 agccgagcac cctgccgtgc gcccgacgcc ggccttctcg agggcctgcc tacgcccgtc
     1861 tcggctggcg aacgcgcatc cgcgacgcgt agtatccacg cggcggcgcc gtccgtcgaa
     1921 aggatcgccc gcgtacccgt cgcgacgagt cttcaccgac cgccggctcc cccaccccgg
     1981 tccggccgcg ttcgccgtcg cagggccatg ccccgcgacg ggcgacgacg cgcggacaca
     2041 ccaccaagga ggcacatcac catgtcgcat cgtatgaagc tggccgtctc tgcgctcgtc
     2101 gcaggtgcgg ccctcgtgtt ggccggctgt tccgcgccgc cgcccagcgc ctcgccgagc
     2161 agctcatcct cgtccggcag caagacggcc tcgtcgaagt tcatgccctg cgtggtctcc
     2221 gacaccgccg ggttcaacga ccactcgttc aaccagttga gcctgcaggc cgtgcaagag
     2281 gcagcgaaaa aggccggcac gacgtacaag atggcgaagt ctcagaccgc gtcagactac
     2341 gtgccgaacg tcaaccagct ggtcgccgcg ggctgcaccg cgacggtcgc ctccggcttc
     2401 aacctcgtgg cggccatcaa gggcgctgcg aaggcgaaca cgaacaaaga cttcgtgatg
     2461 gtggacgaca actcgatcaa cctgccgaac gtgaaagacg tggtgttcgc gacgaaccag
     2521 gccgcgttcc tcggtggcta cgcggcggcg gcctacagca agaccggcgt cgtcgccacc
     2581 tggggcggcc aggagatccc gcccgtgacg atctatatgg acggcttctg ggacggcgtg
     2641 cagtactaca accagcagaa ccacaagaac gtcaaggtct tgggctggaa cgagaagacg
     2701 cagaagggca ccttcgtcgg caacttcacc gatcagaaca cggccaagac cgacacgacg
     2761 aacttcatca accagaaggc tgacgtcatc atgcccgtcg ccggccccct ctacgagggt
     2821 gccggggccg cgatccgcgc caccaaggcg aacgcggtgc tcgtcggggt ggatgccgat
     2881 ctcttcaaga ccgacacgag cggctacaaa gatctcatcc tcctctcagt gatgaagggc
     2941 atccagcccg ccgtcgcgac ggtgctcgac caatccgccg ccgggcagtt ctccgccacg
     3001 cagtacatcg gcacgctgaa gaacaacggc gtcgcgatct ctccgttcca cgacttcacg
     3061 agcaaggtgc cgaagaacct ctccgcgcaa ctcgcgaaga tcaaggcggg catcatcgac
     3121 ggctcgatcc aggcgccgtc gccggccgag ccgaagggct gagtaagaag gatcggaacg
     3181 gccggtgggg cggctcgcac atgccgcccc accggccgcg ggcgtggagg aagagcagag
     3241 gatgagactc gagttacgcg gcatcacgaa acggttcggc tccctggttg ccaacgacca
     3301 catcgatctc gccatcgagc ccggtgaggt gcactgcatc ctgggtgaga acggcgccgg
     3361 caagacgacc ctcatgaaca tgctctacgg cctgttgccg atcgacgagg gcgagatcgt
     3421 catcgacggg gcggcgcagc atttccagag cccgcgcgac gcgatgcatg ccggcatcgg
     3481 catggtccac cagcatttca tgctcgtgcc ggtcttcacc gtcgccgaga acgtcatgct
     3541 cggccacgag gagacgacgt tcgcgggccg cctgaacctt gccgcggcac gcgacacggt
     3601 gcgccgcatc gccgaccagt tcggcttcga tctcgacccc gacgcccgca tcgaagacct
     3661 gcccgtcggc gtgcagcaac gggtcgagat catcaaggcc ttggcgctcg acgcccaagt
     3721 gctcgtcttc gacgagccga ccgcggtact cacgccgcaa gagaccgacg agctgatggc
     3781 gatcatgcgc cggctgaaag aatccggccg cgcgatcgtg ttcatcaccc acaagctccg
     3841 cgaggtgcgc gaggtcgccg atcgcatcac ggtcatgcgg ctcggcaaga tcgtcggcac
     3901 cgtgcacccg ggagcctctg acgaagagat ggcctcgctg atggtgggcc gccccgtgga
     3961 gttgacggtg cagaaaggcg aggccgcgcc cggcgagccg gcgctcgtcg tcaccggtct
     4021 gacgatgcgg gagggcgggg cacgcccggt gctcgacaac gtcacgttca ccgtgcatcg
     4081 cggcgagatc ctcgcgatcg ccggcgtgca aggcaacggc cagacggagc tcgtcgaggc
     4141 gctcttcggc atgcagcccg aggcgaccgg gcgggcgacg atcgacggcc gcaaccttct
     4201 cggggtcggc gcccgggcgg ccatcgagtc gggcgtgggc ttcgtgccgg aagaccggca
     4261 agaagacggc ctcgtgggcg cgttcacgat cgccgagaac ctcatgctga acggatcgtc
     4321 ggccgctccc ttcgtcaggc acggcaactt gcagctgcgc tacctgcggc gtttcgccga
     4381 cgagcgcatc cgcgaattcg acgtgcgcac acaatccgcg gcgaccctgg cccggcagct
     4441 ctccggcggc aaccagcaga aggtcgtgct cgcccgggag ctcagccgcg acttgaggct
     4501 cctgatcgcg gcgcaaccga cccgggggct cgacgtgggc tcgatcgagt tcgtccacga
     4561 acggatcgtt caggcgcgcg acgaaggcgt gcccgtggtc gtcgtctcca ccgagctcga
     4621 cgaggtcgtc gccctcgccg accgcatcgc ggtcatgtac cgcggcacga tcgtcggcat
     4681 cgtgccgccc acgacccccc gcgagaccct cggcttgatg atggccggcc agatgcccga
     4741 cggagtggag aaggcggcat gagcatcgag cagcaagacc cgtcgccggc cggccgcgtg
     4801 gcgggcaccg acgaggcgcc cgacacgcct gccgccttgc cgcacggcgc cgccgccgcc
     4861 gccgccggcg agtcgacacc ggaacccgag tcgcgcagcg cgcgctttct tcgcgaggtc
     4921 acctccggct ccggcatgat cacggtcctc gcgatcctcg tcggcctcgt cgccggcgcg
     4981 gtgttgatcg tcgtgaccaa ctcttccgtg ctcgccgcta tgcagtattt cgcggccgcc
     5041 ccgggcgcca cgttccgggc ggtcggcgac accgtcggcg gcgcctacgc cgccctgttc
     5101 cgcggcggaa tctacgacta cacgcagacg agcttcatcg ccggccttca gccgctgacg
     5161 cagagcatga gcttcgccgt gcccctgatc gcggcggggc tcggcatcgg catcggcttc
     5221 cgtgccggcg tcttcaacat cggcggccaa ggccagatgc tcgccggcgg cgccttcgcg
     5281 ggctggatcg cctacgcgct gcccatgccg gccgcgctgc acatccccgt agcgatcctc
     5341 ggcgcactcg ttggcggcgc gatctgcgcg ggcatcgccg ggctcttgaa ggcgacgacc
     5401 ggcgcgcacg aggtgatcgt gacgatcatg ctcaactacg tcgccctgta catggtcggc
     5461 taccttctga ccacggccct caaagccccc ggctcgaaca acccgatcag cccggccgcc
     5521 ctgccctctg ccgtgctgcc cggcaagacg gccgggctga acctcggttt cgtgctcgcc
     5581 atcgccttga ctctcgccgc ggcgtggctg atgaaccgat ccgcgctggg cttccagttc
     5641 cgggcggtgg gcgagaaccc gcgcgccgcc cgcgtcgccg gcatcagcgt caagaaagtc
     5701 gtgatcctga cgatgatcat ctccggggcg ttcgtcggct tcgcgggcgc ctacgaggtt
     5761 ctcgggcaga cgacgagcgg attcaccaac gtgctcgacg cgggcgtcgg cttcaacgcg
     5821 atcacggtgg cgctgctcgg ccgcaacaag ccgtggggca tcttctgggc ggggattctc
     5881 ttcggcgtct tccaggccgg cggttacgcc atgcaggcct cgcagggcgt cgacatcgac
     5941 gtgatccagg tcgtgcagtc cgcgatcgtg ctcttcatcg ccgcgccgcc cctggtgcgg
     6001 gcgatcttcc gtctgcccga ggccggcggc cgcacgaaac gaacccgttc cccgaagcgc
     6061 acgacgaaag aggcggtggc ccgatgagca cgctcgcgac gacagccgca ccggccggca
     6121 cgggcgaggc cccgctccgg ctgcgtgact ggaaggcgcc gatcctcttc ggcgtcttca
     6181 ccctcttcgc cgtgatcctc ttcttcgcct tcggcaaggg cggggtgtcg acgttcacct
     6241 tcacggcgcc ggcgacagac gtgatccacg tgccggagat ccacatcgcg gcacgacccg
     6301 tcggcatcgt cgcggcgctc gtcgcggtcg cgatgacggt gatcgacgcc cggtgggtgc
     6361 gcacccgccg gaaaaccccg ctttggttca cgacgctctt tgccgtcatt ctcgtcgtcg
     6421 cgttcctcgc ctggtcgacg gtgggccact cgttgccgat cgtgggcctc ctgggcggcg
     6481 ccatcgcact cagcgtgccg ctgatcttcg gggcgttggg cggcgtggtc tcggaacgcg
     6541 ccggggtcgt caacatcgtg atcgaaggcc agttgctcgc cggcgccttc gtctctgcgg
     6601 tggcggcgtc gatgacgcat tcggcgacca tcggcctcat cgccgcggcc gtcgcgggca
     6661 tgctggtctc gttcatgctc gccgcgttct cgataaccta cttcgtcgac cagatcatcg
     6721 tcggcgtcgt cttgaacgtc ttggtcctgg gcctcacgaa cttcatctac cagacctcgc
     6781 tgacgcagaa cccgcagctc ttgaactcgc cgaaacagct gccgaccctg cccatccccg
     6841 gcctctcggc gatccccatc gtggggccgg tgctgttcaa ccagacgatc atcgtctacc
     6901 tcatgtacgg ggtgatcgcg gccgtcttct atgtgctctt cttcaccggt gtggggctgc
     6961 gcatccgtgc cgtcggggag catcccgccg cggccgacac cgtcggcatc aacgtgaaca
     7021 gcacccggtt ctggagcgtc tcgctgggcg gggcgatcgc gggcctcggc ggcgccttct
     7081 tcacgctcgg ctcggtgggc gccttcaccg aagacgtctc cagcggcgaa ggctacatcg
     7141 ccctggcggc cgtgatcttc ggcggctggc accccatcaa ggcgatgttc gccgcgctgc
     7201 ttttcgggct ggccaacgag ctgcagtcga ttctcgggat tctgggctcg cccgtgccca
     7261 gccagttctt gctcatggtg ccctacgtcg tgacgatcat cgccgtcgcg gggctcgtcg
     7321 ggcagtcgcg ggcgcccgcc gccgacggga aaccctactt caaatgacgc gggagggcgc
     7381 cgtgggcggg gttctcg
//
LOCUS       DASTNL010000096         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_997778, whole genome shotgun sequence.
ACCESSION   DASTNL010000096 DASTNL010000000
VERSION     DASTNL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997778"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1218
                     /locus_tag="VFQ96_03160"
     CDS             256..1218
                     /locus_tag="VFQ96_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0256818.1"
                     /translation="MNLRDLSYLVAVAEEGHFGRAAATCFVSQPTLSTQIRKLERELG
                     VILVERARGGALLTTAGRRIVGRARAALEATAEIKEIARLERDPDSTVVRLGILPTSG
                     PYVMPHVLRLIRRRLPRLRLHLVEDKTEPLLDQLRTGGLDAVVCALPVEATDLHTEPL
                     FDEPFVLATPGGHPLAGKPDLVVSDLGDERLLLLEEGHCLRAQALEVCRLAGAREKEQ
                     FRATSLETLREMVAVNVGITLLPVLSVQPPVAPAENVLLTRFTGDVPVRHMAMLWRSS
                     AVIGGLLSALAAAFRSAPGPLLDPRTIPHPAGHDSSWPRQTLAA"
     gene            1333..1938
                     /locus_tag="VFQ96_03165"
     CDS             1333..1938
                     /locus_tag="VFQ96_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HEU0256819.1"
                     /translation="MTEVHGPRRLGPLSWLVGEWEGDVGKDVSYHNKDDETTPTGYFE
                     KAWFRPIPVQENGRQSLEGLNYKMTAWRHGEEAMDPFHDEVGYLLWEKATGQVLRTAV
                     FGRGIAMIAGGDAGLYDEAFTMRATPGDANYGVLQNKYLSERAELRHFECAFTHNADG
                     TVGYRQTLVLRLAALGGIDMEHTDQNTLRRVRRLHPSALVA"
BASE COUNT          344 a          686 c          689 g          307 t
ORIGIN      
        1 aaagaatgcc acacccgcac ggccgcgcca accggttcac ggtgcggcgc cgattttgcc
       61 cgtgtcgcgc tcttcaacgc gacgcgcgcg ggttgcgggc ggtcggacgg cgccgtggcc
      121 ggcgccccgc cgggacccag aaacgcgcac ccggagtcct tcgtagaatg gttccggcgg
      181 gggcggaaag cgcatcggcc ggcccgaaac cgcctatgag gaaggtctgt caggcttgtc
      241 tgtgagcgtt cacccgtgaa cctgcgagat ctctcatacc tcgtcgccgt ggccgaagaa
      301 gggcatttcg gcagggccgc cgccacgtgt ttcgtcagcc agccgacgct gtcgacccag
      361 atccgcaagc tggagcggga gctgggcgtg atcctcgtcg aacgggcccg cggcggcgcg
      421 cttctgacga cggccggcag acggatcgtg gggcgggctc gggccgcact cgaggcgacg
      481 gcagagatca aagagatcgc ccggttggaa cgtgaccccg actccacggt cgtgcggctc
      541 ggcattctgc ccacgtcggg cccgtacgtg atgccgcacg tgctgcggct catccggcga
      601 cgactgccgc ggctgcgcct gcacctggtc gaagacaaga ccgagccgct attagaccaa
      661 ctgcgcaccg gcgggctcga cgcggtggtc tgcgcgctgc ccgtggaggc caccgacctg
      721 catacagagc cgctgttcga cgagccgttc gtgctggcga caccgggcgg gcatccgctg
      781 gccggaaaac cagacctcgt cgtctccgac ctcggcgacg agcgcctcct gcttctggag
      841 gagggccact gtctgcgggc ccaggcactc gaggtgtgcc gcctggcggg cgcccgagaa
      901 aaggagcaat tccgggcgac gagcctcgag acgttgcgcg aaatggtcgc cgtcaacgtc
      961 gggatcacct tgctgccggt actgtccgtg cagccgccgg tcgcccccgc ggagaacgtg
     1021 ttgctcaccc gtttcacggg cgacgtgccg gtacggcaca tggccatgct gtggcgctcc
     1081 tcggcggtga tcggcggcct gctgagcgcc ttggctgcgg ctttccgaag cgccccgggg
     1141 ccgcttctcg acccgcggac catcccgcac ccggccggtc acgactcgtc gtggccccgc
     1201 cagacgctcg cggcctgatc gcagacaggc tcgataggca gcgcctatcg acagcatacg
     1261 ccgaatcgat tggacccccg cttcggcttt tcctagcctg aaaaggcgat gtgttcgcgt
     1321 tggaggcagg caatgacaga ggttcacgga cctcggcggc tcgggccgct ctcgtggctg
     1381 gtcggcgagt gggagggcga cgtcggaaag gacgtctcgt accacaacaa agacgacgag
     1441 accacgccga ccgggtactt cgagaaggca tggtttcgac ctataccggt gcaggagaac
     1501 ggcaggcaga gcctcgaggg cctgaactac aagatgaccg cctggcggca cggtgaagag
     1561 gcgatggatc cgttccacga cgaggtcggc tacctcctgt gggagaaagc gaccggtcag
     1621 gtgctgcgta ccgccgtctt cggccgaggt atcgcgatga tcgccggcgg ggatgccggg
     1681 ctctacgacg aggccttcac catgcgcgcc acgcccgggg acgccaacta cggcgtcctg
     1741 cagaacaagt acctctcaga acgcgccgag ctgcgccact tcgaatgcgc gttcacgcat
     1801 aacgccgacg gcaccgtcgg ctaccggcag accctcgtct tgcgtctggc ggccctgggc
     1861 gggatcgaca tggaacacac cgaccagaac acgctgcgtc gcgtacgacg cctgcacccg
     1921 agcgccttgg tcgcctgaat cccgaatgct ccgccggcgt ccgggcgccg gccaggccga
     1981 ccctagcatg agaccatgca gaatcgcacg gcacaacggt ggggaa
//
LOCUS       DASTNL010000097         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_258438, whole genome shotgun sequence.
ACCESSION   DASTNL010000097 DASTNL010000000
VERSION     DASTNL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258438"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFQ96_03170"
     CDS             complement(<1..344)
                     /locus_tag="VFQ96_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0256820.1"
                     /translation="MTLIEEGAGGATTLLVNGRIWAGAGTPASRREEAIAVAGGRVRA
                     LGGTREVRERFPHAKEIDLAGHTVVPGLIDGHNHAARGGATWASELHWAGLSSREAAL
                     ESIRQAVVKAGSG"
     gene            complement(471..2099)
                     /locus_tag="VFQ96_03175"
     CDS             complement(471..2099)
                     /locus_tag="VFQ96_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0256821.1"
                     /translation="MKEKYDVPVLVVGGSLVGMTTSALLGTQGVTSLVVERHRGAAIH
                     PRAALMLQRSMEIFRSMGVEDTMRERSFQQFDPDGAIMAVETVAGNDLAWYMTRVNDG
                     VRDLSPSERIFVTQIAVEPVLKERAEQLGSEVLYGTEMVDFHQDADGVTARIRDRDTG
                     QEATVRAKYMIAAEGPHSHSRELLGITRSGHGTLSKSITIYFRADVGDLMRGRNLSVI
                     MVVNPEVQGFFRIEKPYESGFLVVHGVGDPENPQTDLWEGLSDEKCVEWVRAALGVPD
                     IPVEIYNVMKWEATAITADRYRDGRVFLVGDSAHEMPPYGGYGGNTGIHDAHNLAWKI
                     AAVLSGAAGDELLSSYEAERRPVGAFTVEQGYSRFVVRAVTSRLKDGIEPVVPDDNID
                     LGYRYRSSAVVAAEGNDAVQGSPRSWRAEPGTRAPNYELLIDGKKTSTIDLFGHGFVL
                     LTGEKAAAWRESGKRASEEVAMGLTTYTIDGEADPTHTFCNEYGITSTGAVLVRPDGF
                     VVWRATTDIDAHPETIAQTMRSVLGLPVSDAVTA"
     gene            complement(2231..2830)
                     /locus_tag="VFQ96_03180"
     CDS             complement(2231..2830)
                     /locus_tag="VFQ96_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU0256822.1"
                     /translation="MGTAILDPITAQNSVVLLVDFQGALFGGVGSGDRDAIHNSLVAT
                     AKAAHILDIPTVLSSIWEGGNGPIIEEVMEQFPGQKVFERAGQSFDAFEDEGVAAAVR
                     ATGRKKLVVSGLWTSMCFAYTALHGIQEGYEVYGLIDAAGDSTPDAHNYGQARMYQAG
                     VIPITWMPLVSHWMHDWNNPKAGQLKAEVFGRYDKMLSI"
     gene            3128..>3978
                     /locus_tag="VFQ96_03185"
     CDS             3128..>3978
                     /locus_tag="VFQ96_03185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0256823.1"
                     /translation="MADTSTTIGWLGTGRMGFQLVRRLLAAGYDVTVYNRTRAKAEPL
                     QEYGARVVDTPVELADRDVVFSMVSASKDLHSVMLGENGLLTGITTPRVICDSSTVSV
                     EAGEELREAAAKTGTAVLATPVSGNPKVIESGKLTVAASGPRSAFEEAEPLLRAFGRG
                     VTYVGEGEVARLVKIAHNVFLGVVTQSLAEITVLAEKGGVSREAFLAFLNDSVMGSVF
                     SRYKTPAFVNLDFTATFTMPLLQKDFDLGLHAGDELGVPMPIASATRQIVAAEAGAGN
                     RESDFATL"
BASE COUNT          590 a         1285 c         1372 g          731 t
ORIGIN      
        1 ccggagccgg ccttgacgac ggcctgccga atgctctcca gcgcggcttc tctcgacgag
       61 aggccggccc aatgcagctc ggatgcccag gtcgcgccgc cgcgcgccgc gtggttgtgg
      121 ccgtcgatca gaccgggcac cacggtgtgg ccggccaggt cgatctcttt cgcgtgcgga
      181 aagcgctccc gcacctcgcg cgtgccgccg agcgcccgca cgcggccgcc ggcgacggcg
      241 atggcctctt cacgtcggct cgccggggtg ccggcaccgg cccagatgcg gccgttgacc
      301 aagagggtcg tcgcgccgcc ggcgccttcc tcgatgaggg tcattctccg ccgccttcgt
      361 tcgtcgttcc tcgccggcct tgccgggccg tacgtctttg atgagccggg atggccgact
      421 gcggggtgcg gcgacggtcc gcagaccgct gccgcacccc ggcgggtgtg tcaggcagtc
      481 accgcatccg acaccggcag gcctagcacg gagcgcatgg tctgcgcgat cgtctcgggg
      541 tgtgcgtcga tgtcggtcgt cgcccgccat acgacgaaac cgtcgggccg caccagcaca
      601 gccccggtgg aggtgatgcc gtactcgttg cagaacgtgt gggtcgggtc cgcctcgccg
      661 tcgatggtgt aggtcgtaag ccccatcgcg acctcctcgc tcgcccgctt gccggattcg
      721 cgccaggcgg cggccttctc tccggtcaga agcacgaagc catggccgaa caggtcgatc
      781 gtcgaggtct tcttgccgtc gatgaggagc tcgtagttcg gcgcgcgggt gccgggttcg
      841 gcacgccacg agcgcgggct gccctgcacg gcgtcgttgc cttcggccgc gacgacggcg
      901 gatgaccgat agcggtagcc caagtcgatg ttgtcgtcgg ggacgacggg ctcgataccg
      961 tctttcaggc gggaggtcac ggctcgcacg acgaagcgcg agtagccctg ctccaccgtg
     1021 aacgcgccaa ccgggcgccg ctctgcctca taggaggaga gcagttcatc gcctgccgcg
     1081 ccggagagca cggcggcgat cttccatgcc agattgtgtg cgtcgtggat gccggtgttg
     1141 cctccgtagc cgccgtacgg cggcatctcg tgggcggagt cgccgacgag aaagacccgg
     1201 ccgtcgcggt aacggtcggc ggtgatcgcg gtcgcctccc acttcatgac gttgtagatc
     1261 tcgaccggga tgtcgggcac gccgagcgcc gcccgcaccc attcgacaca tttctcgtcg
     1321 ctgagcccct cccacaggtc ggtctgcggg ttctcggggt cgcccacgcc gtgcacgacg
     1381 aggaagcccg actcgtacgg cttctcgatg cggaagaagc cctgcacctc ggggttcacg
     1441 accatgatga cgctcaggtt gcgcccacgc atcaggtcgc cgacgtcagc acggaagtag
     1501 atggtgatgc ttttggagag cgtgccgtgg ccgctgcgcg tgatgccgag cagttcgcgg
     1561 ctgtggctgt gcgggccttc cgcggcgatc atgtacttcg cccgaaccgt cgcctcttgc
     1621 ccggtgtcgc ggtcgcggat cctcgccgtc acgccgtcgg cgtcttgatg aaagtcgacc
     1681 atctcggtgc cgtagagcac ctcggagccg agctgttcgg cgcgttcctt caacaccggc
     1741 tcgacggcga tctgcgtcac gaagatgcgc tccgacgggc tgaggtctcg cacgccgtcg
     1801 ttcacgcggg tcatgtacca ggccaggtcg ttgccggcca ccgtctcgac ggccatgatt
     1861 gcgccgtcgg ggtcgaactg ttggaaggag cgctcccgca tggtgtcttc gacgcccatg
     1921 ctgcggaaga tctccatcga gcgttgcagc atgagggcgg cgcgggggtg gatggccgcg
     1981 ccgcgatgcc gctccaccac gaggctcgtg accccctggg ttccgagcaa cgccgaggtg
     2041 gtcatgccga ccaggctgcc gccgacgacc aggaccggta cgtcgtattt ctctttcatg
     2101 gttttctacc cacttcgttg tgtgccgagc ctcgtgatcg cgtatgcgcc gcgccggccg
     2161 gggctccggg ccgggggtgg tcgatggagc tgccggcgcc gtgttggcga ggccggcgat
     2221 gcgtctcggc ctagatggag agcatcttgt cgtagcggcc gaacacctcg gccttcagct
     2281 ggccggcctt cgggttgttc cagtcgtgca tccagtgcga gacgagcggc atccaggtga
     2341 tcgggatgac gcccgcctgg tacatgcgtg cctggccgta gttgtgtgcg tcgggcgtcg
     2401 agtcgccggc cgcgtcgatg agcccgtaca cctcatagcc ttcttggatg ccgtgcaagg
     2461 cggtgtaggc gaagcacatg ctcgtccaca accctgagac gacgagtttc ttgcggcccg
     2521 tcgcgcggac cgccgcggcg accccttcgt cttcgaacgc gtcgaagctc tggccggcac
     2581 gctcgaagac cttctggccg gggaactgct ccatgacctc ctcgatgatc ggcccgttgc
     2641 cgccctccca gatggagctc aagacggtcg ggatgtcgag gatatgggcg gctttcgcgg
     2701 tggcgacgag gctgttgtgg atcgcgtcgc gatcgccgga gccgacgccc ccgaacagtg
     2761 cgccctgaaa gtcgacgagc agcacgacgc tgttctgtgc ggtgatgggg tcgagtatcg
     2821 ctgtgcccat gtgaactcct atgttccagt tgcgaaccag cagccctcct gggccgcgat
     2881 cgattgcaat tatggtaccc cgggccgccc cggtcaagca ccggctggtc agagaatcga
     2941 aaaagtgcgg gatcgcgagt actggccgtt ttatcaggta ttccgtttga ccaaaggtca
     3001 taggagtggc catgaatgtt gggagcacgg tgtttcacgg tcttcttgac cgatgcgaca
     3061 gaccgtagga tgattgcaac cggttgcgtt aagggcgtgg ccacgtgggc acgccgaggg
     3121 aggactcatg gccgacactt caacgacgat cggatggctg ggtaccgggc gcatgggctt
     3181 tcagcttgtg cgccggttgt tggcggcggg gtatgacgtc accgtctata accgcacccg
     3241 tgcgaaggcg gagccgttgc aggaatacgg tgcccgggtc gtcgacaccc cggtggagct
     3301 ggccgaccga gacgtcgtct tctccatggt ctcggcgtcg aaagacctgc attccgtgat
     3361 gctgggcgag aacgggctcc tgacggggat cacgaccccg agggtcatct gtgactcctc
     3421 gaccgtctcg gttgaggccg gcgaggaact gcgtgaggcg gccgccaaga ccggcacggc
     3481 cgtgttggcg acaccggtga gcggcaaccc gaaggtgatc gaatcgggca aactgaccgt
     3541 cgccgcctcc gggccgaggt cggccttcga ggaggcggag ccgctcttgc gcgccttcgg
     3601 ccgtggcgtg acctacgtag gcgagggcga ggtggcgagg ctggtgaaga tcgcgcacaa
     3661 cgtattcctc ggcgtcgtga cgcagtcgtt ggctgagatc accgtgctgg ccgagaaagg
     3721 cggtgtctcg cgcgaggcgt tcctggcctt cttgaacgat tccgtcatgg gctccgtctt
     3781 cagccgttac aagacgccgg cgttcgtgaa cctcgacttc acggcgacct tcacgatgcc
     3841 gcttctgcag aaagacttcg acctcgggtt gcacgccggc gacgagctgg gcgtaccgat
     3901 gcccatcgcc tccgcgaccc gccagatcgt ggcggccgag gcgggggcgg gcaaccgcga
     3961 gtcggatttc gccacgct
//
LOCUS       DASTNL010000098         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_998551, whole genome shotgun sequence.
ACCESSION   DASTNL010000098 DASTNL010000000
VERSION     DASTNL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998551"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFQ96_03190"
     CDS             <1..930
                     /locus_tag="VFQ96_03190"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEU0256824.1"
                     /translation="NVVRVMPPCLGAASITGPERHTCEADPHADPLSGTASPGAAVPS
                     PAAVHEDIPKTCQASPTSSRLNVCSVGVPASSAVRTVALVGDSHAAEWEPALERVANR
                     EGWHVVMLLKGSCAFNTSQRRAGSGSREARSCAAWNRKAAVKLQQIPGLDLVIVSADI
                     KDLYRASEGASGYENAVSGYVAAWRGLPSTVQVDVIADTPRPVPNVVDCLALHRGQAA
                     AATACARPRTAALGDDPLVSAAEELPARVRLVTMTDDFCSATTCDPIIGHVTVYRDQS
                     HISATYSATLAPALRRALAEADKPPRNSTGMGG"
     gene            complement(1023..2762)
                     /locus_tag="VFQ96_03195"
     CDS             complement(1023..2762)
                     /locus_tag="VFQ96_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0256825.1"
                     /translation="MSAAPIPHAWNVAAADTLPGQAPQHAGRNSDFDPSAFRDVFEHR
                     FTWAAAFERNTHRYGNRTALTDSTTGRSWTYRELGVVVGRLVTGLRERGIGTGSIVAY
                     QLMNVPEFAFLYTAAQGLRAVSSPMNFRLAPAEVAHVLDASTPAVFLYDARADVETTK
                     ALKLARHRPDLIVAVGEGEPIAGAIRFAELLDNDGPTFHVPADATVWDETSRLYTSGT
                     TGMPKAVPLTSLNETLSAHDVVMHFPLAPDDKTMNMSPWFHRGGNYCAGPNTMFFVGG
                     ESVTLPHFDPDLVLDHVQNHGLTYVIGAPTNLERLADAQEARPRDLSSLRGIVTMGAP
                     LSHDAALRYQRVLTPRIANGYGTTEAFWNTYLRPEDLPQAAGAAGRACLDDDVAVVRV
                     YADRVATPDDTVAHDGQEIGEVIMRSVKSGYGYMHNPEEEAKKFHHGWLYPGDLATWD
                     DDEMVTVIGRKDDMIISGGENVHPVQVEEVLAGHPDVADSIVVGLPDEEWGELVVAYV
                     QPREGKFTNPQVAATALDAFCRASDLIAGYKKPRRYAFVDELPYTATGKKQHFVMKER
                     TAADAEAGILLTP"
     gene            complement(2861..>3614)
                     /locus_tag="VFQ96_03200"
     CDS             complement(2861..>3614)
                     /locus_tag="VFQ96_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEU0256826.1"
                     /translation="YRAGLRRAALPRRDGPPATAGRWSLLPPPESDPTVLAHARAWRL
                     LDRYGVVTRGAVAAEGVPGGFAATYRILSALEDAGRVRRGYFVEALGAAQFALAATID
                     RLRAQAADAGPNEAEPRQGDAARPEPVLLAATDPANPFGAALPWPKPVGDGPETTHRP
                     ARKAGALVTLHDGAPGLYVERGGKSMLAFTDDEESLAAAARVLADAVRAHRVAAITVE
                     SVNGVPIAHTSAAQGFAGAGFTEVPRGLRMRP"
BASE COUNT          502 a         1267 c         1303 g          542 t
ORIGIN      
        1 aatgtcgtgc gtgtcatgcc gccgtgcctc ggcgccgcca gcatcacggg gccggaacgg
       61 cacacgtgtg aggccgaccc tcatgccgac ccgctctccg gcaccgcctc gccgggcgcg
      121 gcggtgccga gcccggcagc cgtgcacgaa gacatcccga agacctgtca ggcctcgccc
      181 acgagcagtc gactgaacgt ctgcagcgtc ggcgtgccgg cctcgtcggc ggtgcgcacc
      241 gtcgcgctcg tcggcgactc ccatgccgcc gaatgggagc cggccctcga acgcgtcgcg
      301 aaccgcgagg gatggcacgt cgtcatgctg ctgaaaggct cctgcgcgtt caacacgtcg
      361 cagcgcaggg ccggcagcgg cagccgggag gcccgcagct gcgccgcctg gaaccgcaag
      421 gcggcggtga aactgcagca gatcccgggc ctcgacctcg tcatcgtctc tgccgacatc
      481 aaagacctgt atcgggcctc ggagggcgcc agcggctacg agaatgcggt gtcggggtac
      541 gtggcggcct ggcggggcct gccctccacc gtgcaggtcg acgtgatcgc cgacacgccc
      601 cggccggtgc ccaacgtcgt ggactgcctg gccttgcacc gcgggcaggc cgcggcggca
      661 acggcgtgtg cccggccccg cacggcggcg ctcggcgacg acccgctcgt ctccgccgcc
      721 gaggaactgc ccgcccgggt gcgccttgtc acgatgaccg acgacttctg ctcggcgacg
      781 acctgcgacc cgatcatcgg ccacgtcacc gtctaccggg accaaagcca catcagcgcc
      841 acctattcgg cgacgctcgc cccggccctg cgccgggctc tggccgaggc ggacaagccg
      901 ccgaggaact ccaccggcat gggcggctag ccgctcgcct tccgcccggg gcacccgggc
      961 atcggtgcgg cggcggagcc gagtgttccc ggccgggtgg cgtggccgtc ggagtcagag
     1021 ggtcaaggag tgaggaggat gccggcctcg gcgtctgccg cggtgcgctc tttcatgacg
     1081 aagtgctgct ttttgccggt cgccgtgtag ggcaactcgt cgacgaacgc gtagcggcgc
     1141 ggcttcttgt agccggcgat caggtcgctc gcccggcaga acgcgtcgag cgccgtcgcc
     1201 gcgacctggg gattcgtgaa cttgccttcg cggggctgga cataggcgac gaccaactcg
     1261 ccccactcct cgtcaggcag gccgacgacg atgctgtccg ccacatccgg gtgcccggcg
     1321 agcacctctt ccacctgtac cgggtgcacg ttctcgccgc cagagatgat catgtcgtct
     1381 ttgcggccga tcacggtcac catctcgtcg tcgtcccagg tggcgaggtc tcccgggtac
     1441 agccaaccgt ggtggaactt cttcgcctct tcctccgggt tgtgcatgta tccgtagccg
     1501 cttttgacgg agcgcatgat gacctcgccg atctcttgac cgtcgtgcgc gaccgtgtcg
     1561 tcgggggtgg ccacgcggtc ggcgtacacg cgcacgacgg cgacgtcatc gtcgaggcac
     1621 gcccgcccgg ccgcgccggc cgcctggggc agatcctccg gtcgcagata ggtgttccag
     1681 aacgcctccg tcgtgccgta cccgttcgcg atgcgcgggg tgagcacccg ctggtaacgc
     1741 agtgccgcgt cgtggctgag cggcgcgccc atcgtcacga tgccgcgcag cgacgacagg
     1801 tcgcggggcc tggcctcttg tgcgtcggcg agacgctcca ggttggtcgg cgcgccgatg
     1861 acataggtga ggccgtggtt ttgaacgtgg tcgagcacga gatcggggtc gaaatgcggc
     1921 agcgtgaccg attcgccgcc gacgaagaac atcgtgttcg gccccgcgca atagttgccg
     1981 ccgcggtgga accacggcga catgttcatc gtcttgtcgt cgggtgcgag cgggaagtgc
     2041 atgaccacgt cgtgtgcgga aagggtctcg ttcaggctgg tcagaggcac cgccttcggc
     2101 atgccggtcg tgcccgaggt gtagagacgc gacgtctcgt cccacacggt cgcgtcggct
     2161 ggaacgtgga aggtcggccc gtcgttgtcg agcagctccg cgaaacggat ggcgccggcg
     2221 atcggctcgc cctcgccgac cgccacgata aggtcgggcc ggtgccgcgc gagcttcagc
     2281 gccttcgtcg tctcgacgtc ggcgcgtgcg tcatagagga acacggccgg cgtggaggcg
     2341 tcgagcacgt gcgcgacctc cgccggcgcc aggcgaaagt tcatcggcga gctgacggca
     2401 cgcagcccct gggccgccgt gtacaagaac gcgaactcgg gaacgttcat cagctggtag
     2461 gcgacgatgc tgcccgtgcc gatgccgcgc tcgcgcagcc ccgtgaccag gcggccgacg
     2521 acgacgccga gctcccggta tgtccacgac ctgccggtcg tcgagtcggt cagtgcggtg
     2581 cggttgccgt agcggtgggt gttgcgctcg aaggccgctg cccacgtgaa gcggtgttcg
     2641 aagacgtcgc gaaacgcgga ggggtcgaaa tcgctgttcc gcccggcgtg ttgtggcgcc
     2701 tgacccggca gggtatcggc tgccgcaacg ttccaggcgt ggggaatggg ggccgcagac
     2761 atgaaaacct cacttcgtcg cgtcgcacgc cgcgtcattg cgggcgtgca gggtcacggc
     2821 aagaccccgc ggcacagcct acgcccggcc tcgtagaccg tcatggccgc atcctgaggc
     2881 cccgcggcac ctccgtgaaa ccggcgcccg cgaacccctg ggcggcagac gtgtgcgcga
     2941 tggggacacc gttcacgctt tccacagtga tggccgcgac ccggtgggcg cgcaccgcgt
     3001 cggcgagcac ccgcgcggcg gcggccagcg attcctcgtc gtcggtgaag gcgagcatgc
     3061 tcttgccgcc gcgctcgacg tagaggccgg gagcgccgtc gtgcagcgtg acgagcgcgc
     3121 cggccttgcg cgcggggcgg tgcgtcgtct cgggcccgtc gccgacgggc ttcggccagg
     3181 gcagggcggc gccgaacggg ttcgccgggt cggtcgccgc gagaagcacc ggttccggtc
     3241 gtgccgcgtc gccctgccga ggttcggcct cattcggccc tgcgtcggcc gcttgggcgc
     3301 gcagccggtc gatcgtcgcg gcgagcgcga actgggccgc ccccaacgcc tcgacgaagt
     3361 agccgcgtcg cacccggccg gcgtcttcca acgcgctcaa gatccgatag gtggcggcga
     3421 agccgcccgg cacgccctcg gccgcgaccg cgccgcgggt gacgaccccg tagcggtcga
     3481 ggaggcgcca ggcgcgggcg tgcgcgagca cggtcgggtc ggattcgggc ggcggcagga
     3541 gcgaccagcg tcctgccgtc gcgggcggcc cgtctcgccg gggcagggcg gcccggcgca
     3601 ggcccgcccg gtac
//
LOCUS       DASTNL010000099         5820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1187382, whole genome shotgun sequence.
ACCESSION   DASTNL010000099 DASTNL010000000
VERSION     DASTNL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187382"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1533)
                     /locus_tag="VFQ96_03205"
     CDS             complement(91..1533)
                     /locus_tag="VFQ96_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256827.1"
                     /translation="MSVEHRVDTSAGGPGTPGAPTPQIHLVVVFTTVALALMMMSIDS
                     TIVATALHTLQHGLHTSINWAGWTITAYSFGFVVMLPVTGKLSERFGRRKVFLVSVVA
                     FTAASLACGLSVNIFMLIALRAIQAAGGAGFTPSATGIVVDYFGAARDRAVSLFGSIF
                     PIGAMIGPIFGGLFVAYWTWRGVFLINVPVGIAIIALTLRYVPRDRQASADAARREHL
                     PLGVPGMVLLGVGILAGMLAAGTLGEADDSHGVLSPVFLVPALVAIVALVWFFRHISH
                     TAHPFIDPRFIYGRNFGTVNLVNALYSGVRSGAIALLPLYAANRYGIGALSAGTLLIA
                     QGAAAIILSVLAALALRRTGHRPPIYVGSALATLGLVLFALQPIGLSPYWWLAAAAFL
                     IGAGSGASNPASRNAGLQLAPEHSSTLAALRTMSLQIGQIVTIAIVTAVLTGASDPTL
                     TQGWAYLAIAAVFLVCLPIIHRIPEHRGAW"
     gene            1652..3682
                     /locus_tag="VFQ96_03210"
     CDS             1652..3682
                     /locus_tag="VFQ96_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0256828.1"
                     /translation="MVRGGMHHGGARVSETERIDVAALAETLLGRWSAVRRSARALAA
                     RPDLWKREGLSLAAERQHTLSRMSILSRDAMARRAFPTRFGGDADNGGNVVGFEELTA
                     ADPSLQIKAGVQWGLFGSAVLQLGTPEQHDRLLPDILSFRVPGAFAMTEIGHGSDVAN
                     LATTATYDPERQEFILDTPFRAAWKEFLGNAGSDARAATVFAQLVTDGTRHGVHCFYV
                     PIRDEEGRFLPGVEGLDDGQKGGLNGVDNGRLAFHEVRVPRENLLAKYGAVAPDGTYS
                     SPIRSAGRRFFTMLGTLVQGRVSLDGASVVAAKIALAIAVEYADQRRQFAGPGGLDRV
                     LLDYPAHRRRLLPLVARTYAASFAHERLLGVFDDVFGGRADDDAARQNLETMAAALKP
                     LSTWHALETLQQCREACGGAGFMTENRFTSLRADLDVYVTFEGDNTVLLQLVAKRLLS
                     DYAARLKEAGPAAAREVLLGAAQRAVGHSGLRSLGQRVRDVARSLGGGVAWLDEEDTQ
                     RQLLTDRVETIVAETAGRLHARPGASRDAAVTAEQDSLIEAARAHGELLQWEAFTDAL
                     PTIADPATRQILTRLRDLFGLGLIEEHAAWYLMNGRLAPERARAVGARVTRLLRTLRP
                     HALDLVHAFGYGPEHLRATIATGIERERQEEAQAHRDGSAADAETRAAPHAG"
     gene            3850..>5820
                     /gene="aroA"
                     /locus_tag="VFQ96_03215"
     CDS             3850..>5820
                     /gene="aroA"
                     /locus_tag="VFQ96_03215"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0256829.1"
                     /translation="MHLSVAGTAGRVYGEVQVPNSKYHAHRALILASLADGVSRITGL
                     SDARHVQYTVALLRDLGVTITIDHDTFVVHGCGGTYHPRGESVSAGSSGTTLYFMVGL
                     ASLADADVVVTGQKYFQRRPIGPLLAALRDLGVDASSPTDCPPIQLRHGRPSGGEVHI
                     AGMLSQWVSALLLVAPFATKATTIVVDGEFNERSYIDLTVRMMAQFGLQVTVSADGRR
                     YDVAPGQSARPAEIVMPPDIGSAAFGLALAGIHPADVLLAGLHTTEPGETDHPEGDFL
                     GIVRDMGLPMLYEPGSGGVRVRHDGVRLRATEIDCRRVPDMLPILSTMAAFADGETVF
                     HNVEHVRLKESDRVVAMLQLNEMGGEVDFDGTDLRIRGVERLRAAHLSSFNDHRVLMS
                     LAVAATAAEGRSTLTYPNAYRISYPRFLEAMNTLHVDMQVEKRPAKPTSELPRPEGTA
                     KEASRVTGPEWIRRWAKEKPHAVAVVDAAPGRTTNVSWAELDQAVDKAAALFLSLGVG
                     PGDRVGVQLPNCTEFVVIAAAAMRIGAIVCPIMPIFRQREVSFALRRSQAKLLIVVDN
                     FRGRQHDRETASILDDPEARAGLAVENVLVLHATPGVAHVLPAHENRAVTWQAWHRAI
                     GQSVVDRQAIDARAPKPSDAAQLLFTSGTSGEP"
BASE COUNT          917 a         2039 c         2038 g          826 t
ORIGIN      
        1 ttcagactat ctgtccccgc cctgccgtac caccggcgca tccccccgcc cggccgaccg
       61 gcccggcgga gtctcggtgg ctcgtacggg tcaccacgcg ccgcgatgct ccgggatacg
      121 gtggatgatc ggcaggcaca cgaggaacac cgcggcgatc gcgaggtacg cccagccctg
      181 tgtgagcgtc gggtcggagg ctccggtcaa gactgccgtc acgatcgcga tcgtcacgat
      241 ctgcccgatc tgcagcgaca tggtgcgcag cgcggcgagc gtggaggaat gctccggtgc
      301 caattgcagg cccgcgttgc ggctggccgg gttgctcgcc ccgcttcccg cgccgatcag
      361 gaatgcggcg gcggcaagcc accagtatgg gctgaggccg atcggctgca aggcgaagag
      421 tacgaggccg agcgtggcca aggcgctgcc gacgtagatg ggtggccggt gcccggtgcg
      481 gcgcagcgcg agcgccgcca acacggaaag gatgatcgcc gcggcgccct gcgcgatgag
      541 cagcgtgccg gcgctcagcg ccccgatgcc gtaacggttc gccgcgtaca gcggcagaag
      601 cgcgatcgcg ccgctgcgca cgccgctgta cagggcgttg acgaggttga cggtgccgaa
      661 gttgcgcccg tagatgaacc tcgggtcgat gaaggggtgg gccgtgtggg aaatgtggcg
      721 aaagaaccag accagcgcga cgatcgcgac gagcgccgga acaaggaaca ccggcgagag
      781 cacgccgtgg gagtcgtcgg cctcgcccag ggtgcccgcg gcgagcatcc cggcgaggat
      841 gccgacgccg aggagcacca tgccgggcac gccgagcggc aggtgttcgc ggcgcgcggc
      901 gtcggcggag gcctgccggt cgcgcggcac atagcgcaac gtcaacgcga tgatcgcgat
      961 gccgaccggc acgttgatga ggaacacacc ccgccaggtc caatacgcga cgaacaagcc
     1021 gccgaagatc gggccgatca tcgccccgat cggaaagatg ctgccgaaca ggctgaccgc
     1081 acggtcgcgg gcggcgccga agtagtcgac cacgatgccg gtggcagacg gggtgaagcc
     1141 ggcgccgccg gccgcctgaa tggcgcgcaa cgcgatgagc atgaagatgt tcaccgacag
     1201 cccgcacgcc agcgacgccg ccgtgaacgc gacgacggag acgaggaaga ccttgcgccg
     1261 gccgaagcgc tcgctcagct tgccggtgac gggcagcatg acgacgaagc cgaacgaata
     1321 ggcggtgatc gtccagccgg cccagttgat cgaggtgtgc aggccgtgct gcagggtgtg
     1381 caacgccgtc gcgacgatcg tcgagtcgat cgacatcatc atgagggcca gagcgacggt
     1441 cgtgaagacg accaccaggt ggatctgcgg cgtcggggcc ccgggtgtgc ccggcccgcc
     1501 ggcgctggta tcgacgcggt gttcaacgct cactggtctt agtttagcca gcctaagttt
     1561 cttgtgcctg agcgctagag gcgcccgggc cgcgcgggta ggctgctgcc agctgcacgg
     1621 cccgcggcat tcgcgtcgcg ccctgccggc aatggttcgc ggaggaatgc atcatggcgg
     1681 agcccgagtg agtgagaccg aacggatcga cgtggcggcg ctcgccgaga cactgctcgg
     1741 ccgctggtcg gcggttcgtc gctcggctcg cgcgctcgcg gcccgccccg atctgtggaa
     1801 gcgcgaaggc ctctccctcg ccgcggagcg gcagcacacg ctctcgcgta tgagcatcct
     1861 ctcgagagac gccatggccc gccgtgcgtt cccgacccgg ttcggcggcg acgccgacaa
     1921 cggcggcaac gtcgtcggct tcgaagagct gaccgccgcc gatccctcgt tgcagatcaa
     1981 ggcgggtgtg cagtgggggc tcttcggctc ggccgtcttg cagctcggca cccccgaaca
     2041 gcacgaccgg ctgctgcccg acatcctgag ctttcgggtg cccggggcgt tcgccatgac
     2101 ggagatcggc cacggctccg acgtggcgaa tctcgcgacg accgccacct acgaccctga
     2161 gcgtcaagag ttcatcctcg acacaccgtt ccgtgccgcg tggaaggagt tcctcggcaa
     2221 cgccggcagt gacgcgcgtg ccgccaccgt gttcgcgcag ctcgtcaccg acggcacgag
     2281 acacggcgtg cactgcttct atgtgccgat ccgcgacgag gaggggcggt ttctgcccgg
     2341 ggtggaaggt ctcgacgacg ggcagaaagg cgggctgaac ggcgtcgaca acgggcgcct
     2401 cgcctttcac gaggtgcggg tgcctcgcga gaacctgctg gccaagtacg gggcggtcgc
     2461 ccccgacgga acctacagtt ccccgatacg aagtgccggc cgccggttct tcacgatgct
     2521 cggcaccctc gtgcaaggcc gcgtctccct cgacggcgcc agcgtcgttg cggcgaagat
     2581 cgccttggcg atcgcggtgg aatacgccga ccagcgccgc cagttcgccg gccccggcgg
     2641 tctcgaccgg gtgctcctcg actacccggc gcaccgtcgt cgtctgctgc cgctcgtcgc
     2701 ccgcacctac gcggcgtcgt tcgcgcacga acgcctgctc ggcgtcttcg acgacgtctt
     2761 cggcgggcgg gccgacgacg acgcggcccg tcagaacctg gagacgatgg ccgccgcact
     2821 gaagccgctt tctacttggc acgccctcga gacgctgcag cagtgccggg aggcgtgcgg
     2881 cggggcgggg ttcatgaccg agaaccgctt cacgtcactg cgagccgacc tcgacgtcta
     2941 cgtcacgttc gagggcgaca acacggtgct tctgcagctg gtggccaagc gcttgctgag
     3001 cgactacgcg gcccggctga aagaggccgg ccccgccgcc gcgcgagagg tgctgctggg
     3061 cgcggcccag cgggccgtcg gccacagcgg cctgcgttcc ctcggccaga gggtgcgtga
     3121 cgtggcccgc agcctcggcg gcggcgtggc ctggctcgac gaagaggaca cgcagcggca
     3181 gcttctcacc gaccgggtgg agacgatcgt cgccgagacc gccggtcggc tgcacgcccg
     3241 gccaggcgcc tcgcgagacg ccgcggtgac ggcggaacag gacagcctga tcgaagcggc
     3301 ccgcgcccac ggcgagctcc tgcagtggga ggcgttcacc gacgcgctgc cgaccatcgc
     3361 cgacccggcc acccggcaga tcctcacgcg gctgcgcgac ctgttcgggc tgggcctcat
     3421 cgaagaacac gccgcctggt acctcatgaa cggccgtctc gcccccgagc gggcccgcgc
     3481 cgttggcgct cgggtcaccc ggctgctgcg caccctccgg ccgcacgcgc tcgacctggt
     3541 gcacgcgttc ggctacgggc cggagcactt gcgtgccacg atcgccaccg gcatcgagcg
     3601 ggaacgacaa gaagaggcgc aggcccaccg cgacggctcc gcggccgacg ccgagactcg
     3661 ggcggcgcca catgccggtt aaggcatgaa cgaaggccgg cgggcgggcc gcgggccaag
     3721 acacgggccc ggccggcgcc gagtttgcca cggcggcgcg ccccggtagc gtagattggc
     3781 gctttgacag tctgtgcgct gttccgtgct gcccgtccgg tgagcggcgc gccagagagg
     3841 gagtcggtta tgcacttgtc cgtcgcgggc accgcaggca gagtctacgg cgaggtacaa
     3901 gtacccaact ccaagtatca cgcgcaccgc gcgctcattc tcgcgtccct ggccgacggg
     3961 gtgagccgca tcacggggct cagcgatgcc cgccacgtgc aatacacggt cgcgctgctg
     4021 cgcgacctcg gcgtcacgat cacgatcgac cacgacacct tcgtcgtaca tggctgtggc
     4081 ggcacatatc acccgcgggg agagtcggtc agtgccggca gctccggcac cacgctgtat
     4141 ttcatggtcg ggctcgcctc tctcgccgac gccgacgtcg tcgtcacggg gcagaaatac
     4201 tttcaacgcc gaccgatcgg cccgctcctg gcggccttgc gcgacctcgg ggtcgacgcc
     4261 tcctcgccga ccgactgccc gcccatccag ctgcggcacg gcaggccgtc gggcggcgag
     4321 gtgcatatcg ccggcatgct ctcccagtgg gtctctgccc tgctgctcgt cgcgccgttc
     4381 gcgacgaagg cgacgacgat cgtcgtcgac ggcgagttca acgaacgcag ctacatcgac
     4441 ctgacggtgc gcatgatggc gcagttcggc ctgcaggtca cggtctcggc cgacggtcgg
     4501 cgctatgacg tcgcgccggg ccagagcgcc cgcccggccg agattgtgat gccgcccgat
     4561 atcggctcgg cggccttcgg gctggcgctg gccggcatcc accccgccga cgtgctgttg
     4621 gccggcctgc atacgacaga gccgggcgag acggatcacc ccgaaggcga ctttctcggc
     4681 atcgtgcgcg acatggggct gccgatgttg tacgagccgg gctccggcgg cgtgcgggtg
     4741 cggcacgacg gcgtgcgctt gcgcgccacc gagatcgact gccgacgggt gcccgacatg
     4801 ctcccgatcc tctccacgat ggcggcgttc gccgacggcg agaccgtgtt ccacaacgtc
     4861 gagcatgtgc gcctgaaaga gagcgaccgg gtcgtcgcca tgctgcaact caacgagatg
     4921 ggcggcgagg tcgacttcga cggcaccgac ctgcgcatcc gcggtgtgga gcgcctgcgc
     4981 gccgcacacc tttcctcttt caacgaccac cgggtgctca tgtcgctggc cgtcgcggcg
     5041 acggcggcgg agggccgctc caccctgacc tacccgaacg cataccgcat ttcttaccca
     5101 cgattccttg aggcgatgaa cacgctgcac gtcgacatgc aagtagagaa acgacccgcc
     5161 aagccgacct cggaactgcc ccggccggag ggcaccgcca aagaggccag cagggtgacg
     5221 gggcccgaat ggatccgccg ctgggcgaag gagaagccgc acgccgtcgc cgtcgtcgac
     5281 gcggcgcccg gccgcacgac gaacgtcagt tgggcggaac tcgaccaggc cgtcgacaag
     5341 gccgccgcgc tgttcctcag cctgggcgtc gggccgggcg accgggtggg cgttcagctg
     5401 ccgaactgca ccgagttcgt cgtcatcgcc gccgcggcga tgcgcatcgg ggcgatcgtc
     5461 tgcccgatca tgccgatctt ccggcaacgc gaggtcagct tcgcgttgcg gcgctcgcag
     5521 gcgaagctct tgatcgtcgt cgacaacttc cgcggccgcc agcacgaccg tgagaccgcc
     5581 tcgatcctcg acgacccgga ggcgcgggcg gggctcgccg tcgagaacgt gctcgtcttg
     5641 cacgccaccc ccggcgtggc ccacgtgctg cccgcgcatg agaaccgtgc cgtcacgtgg
     5701 caggcctggc atcgagcgat cggccagagc gtcgtcgacc gccaggcgat cgacgcccgc
     5761 gcgccgaagc cgtcggatgc ggcgcagctg ctcttcacct ccggcacctc cggcgagccg
//
LOCUS       DASTNL010000100         7057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1373336, whole genome shotgun sequence.
ACCESSION   DASTNL010000100 DASTNL010000000
VERSION     DASTNL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373336"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="VFQ96_03220"
     CDS             <1..1997
                     /locus_tag="VFQ96_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0256830.1"
                     /translation="QGTWRTVVDAVHAEGGRIALQLFHAGRYASERSFGLVPVAPSAV
                     YSRFSRCMPEALTDDGIRRTIADFGAGAAVAARLGFDAIEVMGSEGYLINQFASPVTN
                     KRDDAWGGDAERRRAFPVAVLQAVARAAGGLPVIARTSAADLIEGSSTPDEADALAVA
                     LVRAGAAAVSVGIGWHESRVPTVQTLVPHVAWGSVAGRMRAALRAAGEAVPLIASNRI
                     NTVLQAEHVLVKGQADMVSMARPFLADPHIVATSFAGNAAFVNPCIGCNEACIDRSFG
                     LEPVSCTVNPRAGRELEFPSDTQGADPTGADLAARAEPLPGVVGRIVSSGVLGAGGRA
                     DAGAETKGSRRGPGLRDARRGRLVAVVGAGPAGMEAARAAAFAGARVCLFEAQERIGG
                     QFLMAGLVPGKSDFIGTARYFERELRRRGVTVETGHAVAGAEELVGFDHVVLAAGVSP
                     RRVTLPGLGLPGTPPVVAYPEAFRHPERIGRRVAVVGAGGIAVDVAHFLVEEPLPALP
                     WEGQEEERRRRFRAAYRLSAEKAPAPAREITIMRRSGQIGAGMGLTTRWAVVQAIRAA
                     GVRTLTGIRYVGVEAGGIRIAIDGEEELVAADTIVVAAGQVPNDGLAAQLAAANVAHT
                     VIGGALMTSGTNAVAAFEQGLKAGDAAARNMPNSEYVGVE"
     gene            1994..3235
                     /locus_tag="VFQ96_03225"
     CDS             1994..3235
                     /locus_tag="VFQ96_03225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0256831.1"
                     /translation="MSEDERRIVITGLGAITPIGNTVAETWESLVHGVSGVERITAFD
                     PSGCLTQIAAEVKNFDPGEALSVKQVKRMARTSQLAVVAAREAARDAGFDTDFEGQEV
                     GVIVNSAVSGFAEIQDATEVYHSQGERRMSPRFVPSALTNMPACEIAIDMNIHGPVNA
                     SALACASGAYAMIEARRILLAGEADVMFAGGADAAITPVMFQGLNAMHALSKHNDVPA
                     EASRPFDADRDGFVFGEGAVVLTLETLAHAKARGARVYAEVLGGALTSDAFNVVAPEP
                     TAKYATEAVVKGLRKAGLHPSDIDLVCAHGTSTRANDRTETAAIRQGLGEAAYDVRVT
                     APKSMTGHLIGAAGALAGMLCALSITKSVIPPTINYETPDPDCDLDYTPGFAREASVR
                     HAITNAFGFGGQNCVVAFGAV"
     gene            3607..3789
                     /locus_tag="VFQ96_03230"
     CDS             3607..3789
                     /locus_tag="VFQ96_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEU0256832.1"
                     /translation="MDVASGDRIVIRGKAVGNPDRRGTVLEVRGDEGQGPYVIRFDDG
                     RESLVYPGPDFVVTPE"
     gene            complement(3776..5434)
                     /locus_tag="VFQ96_03235"
     CDS             complement(3776..5434)
                     /locus_tag="VFQ96_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEU0256833.1"
                     /translation="MHAHATNHDRRRTWVLLFGGLLAAGALLTAMTPPAPMTPGIGGT
                     PTSRSARALPTDSVAAGTRATSNAPVTLDAQTASATPIVTVPATSPESAPSARLVPAP
                     AGSRAAGPMTLAQAQAGHLAPARTPNWAGYVATGGVFTAVTGTWQVPTLTCGTTTSYS
                     AQWVGIDGYGNGSVEQAGVNAFCHDGAAHYNAWWEMYGDQAENGGASVALPAADAVRP
                     GDTVTAAVRVAGNRWTLTMRDTSAGWSFTKTVADFAAAQASAEWIVERPSICASASNL
                     SSCTATSLAPSDPITLTSATATREAGGHGSARKSAATAPAESFHPISIEMADPKTGSV
                     LAVPSSLAGGSFTVSPLVGRSGKAPIVDEDGRCTTRPFTDVATTGPFCGPIAWLKASG
                     ITTGFKNGAFHPAAPISRAAMAAFLYRRADDGQGATIRCTVRPFSDVPTTSPFCGPIT
                     WLKENGITTGFANGTFQPAAPISRAAMAAFLYRSQTSETHAPACTAEPFADVPTASPF
                     CGSIAWLRQQHVTDGYADGSYRPAKAISRAAMAAFLYRVATTQE"
     gene            complement(5625..6248)
                     /locus_tag="VFQ96_03240"
     CDS             complement(5625..6248)
                     /locus_tag="VFQ96_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEU0256834.1"
                     /translation="MSDVTVIGTGNVGSAVAAITAKGGASVQVLGRDQEKASALAAQV
                     GGTAGSVGDPITGEVVVLAVPYAALAELAQAYGAQLDGKVVVDVTNPVDPTTFDGLTV
                     PADSSAAAELQALVPGAKVLKAFNTNFAGALASGQVGEVPATVLVAGDDADAKQQLVD
                     LVTAGGVAAASVGSLKRARELEAFGFLQITLAAQEVIGWGGGFALRK"
     gene            6350..6871
                     /locus_tag="VFQ96_03245"
     CDS             6350..6871
                     /locus_tag="VFQ96_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0256835.1"
                     /translation="MQQKVPRMDATEARAWVALVSVAELLPATLDQQLTDDIGLINFE
                     YGILSMLNVADGQTLRMGELAAAVKSPAPRLSKAVTRLERKGFVERASCAGDGRAINV
                     RLTRDGRRVWLSATPPHISLARDTILGDLTPDELTRLAELLEVIKARLDPDASLGRIP
                     ERAAPTHQPDHSL"
BASE COUNT         1014 a         2375 c         2570 g         1098 t
ORIGIN      
        1 accaggggac ctggcgcacc gtcgtcgacg cggtgcatgc cgagggcggg cgcatcgccc
       61 tgcaactttt tcacgcgggc cgttacgcgt ccgaacggtc cttcggactg gtcccggtgg
      121 caccctccgc cgtgtacagc aggttctcgc ggtgcatgcc cgaggccctc accgacgacg
      181 ggatccgccg gacgatcgcc gatttcgggg ccggggccgc cgtggccgca cggctcggct
      241 tcgacgcgat cgaggtgatg ggctccgaag gctacctcat caaccagttc gcttcgccgg
      301 tgacgaacaa gcgggacgac gcgtggggcg gtgacgcaga gcgccggcgg gcgttccccg
      361 tcgcggtgct ccaggccgtc gcccgggcgg ccggcggcct gccggtcatc gcgcgcacct
      421 ccgccgccga cctcatcgaa ggctccagca cccccgacga ggcagacgca ctggccgtcg
      481 ccctggtgcg ggcgggcgcg gcggcggtca gcgtgggcat cggctggcac gagtcgcggg
      541 tgcccaccgt gcagaccctc gtgccgcacg tcgcgtgggg gagcgtcgcc gggcgcatgc
      601 gcgccgccct gcgcgctgcc ggcgaggccg tgccgctcat cgcctccaac cgcatcaaca
      661 ccgtcttgca ggcggagcac gtgctggtca aggggcaggc cgatatggtc tcgatggcga
      721 ggccgttcct cgccgacccg catatcgtcg ccacctcgtt cgccgggaac gcggcgttcg
      781 tgaacccgtg catcggctgc aacgaggcat gcatcgaccg cagcttcggc ctggagccgg
      841 tctcgtgcac ggtcaatccg cgggccggcc gcgagctcga gttcccctcc gacacccagg
      901 gcgccgaccc gaccggcgcc gacctggcgg cacgggcgga gccgctgccg ggcgtggtcg
      961 gccgcatcgt gagctccggc gtgctcggcg ccggcggccg cgccgatgcc ggggcagaga
     1021 cgaagggcag caggcggggc ccgggccttc gagacgcccg gcgcggccgg ctggtcgccg
     1081 tcgtcggcgc cggccccgca gggatggagg cggcccgcgc ggcagccttc gccggcgccc
     1141 gggtgtgcct cttcgaggcg caagaacgca tcggcggcca gttcctcatg gccggcctgg
     1201 tgccgggcaa gagcgacttc atcggcacgg cccgctactt cgagcgcgag ttacgtaggc
     1261 gtggtgtcac ggtggagacc ggtcacgccg tggccggcgc cgaagagctc gtcggctttg
     1321 accacgtggt tctcgccgcc ggagtgagtc cgcgtcgcgt gacgctgccc gggctcggcc
     1381 tacccggcac cccgccggtc gtcgcctacc cggaggcgtt ccggcacccg gagcggatcg
     1441 gccgtcgggt ggcggtcgtc ggcgccggcg gcatcgccgt cgacgtcgct catttcctgg
     1501 tcgaggagcc gctgcctgcg ctcccctggg agggccaaga agaggaacgt cgccgccggt
     1561 tccgggccgc ctaccggctg agcgccgaga aagcgccggc acccgcgcgc gagatcacga
     1621 tcatgcgacg ttccggccag atcggcgcgg gcatggggct gacgacccgg tgggccgtcg
     1681 tgcaggcgat tcgtgcggcc ggcgtgcgca ccctgaccgg catccggtac gtgggtgtgg
     1741 aggccggcgg catccgcatc gccatcgacg gcgaggagga gctcgtcgct gccgatacaa
     1801 tcgttgtcgc ggccggccag gttcccaacg acggcctcgc cgcgcagctt gccgcggcga
     1861 acgtcgctca tacggtcatc ggcggggcac tcatgacctc cggcaccaac gcggtcgcgg
     1921 ctttcgaaca gggcctcaag gccggcgacg ccgccgcacg gaacatgccg aattcggaat
     1981 atgtaggagt ggaatgagcg aagacgagcg acgcatcgtc atcaccgggc tgggcgcgat
     2041 cacgccgatc ggcaacacgg tcgccgagac ctgggagagc ctggtgcacg gcgtcagcgg
     2101 ggtggagcgc atcaccgcgt tcgacccgag cggctgcctc acccagatcg ccgccgaagt
     2161 gaagaacttc gacccgggtg aggctctttc cgtcaagcag gtcaaacgca tggcgcgcac
     2221 ctcgcagctg gcggtcgtcg ccgcccgcga ggcggcccgc gacgccgggt tcgacaccga
     2281 tttcgaaggc caagaagtcg gcgtcatcgt caacagcgcc gtgtcgggct tcgccgagat
     2341 ccaagacgcc accgaggtgt atcactcgca gggggagcgc aggatgagcc cgcgtttcgt
     2401 gccgagtgcc ctgacgaaca tgccggcgtg cgaaatcgcg atcgacatga acatccacgg
     2461 cccggtcaat gcgagcgcgc tggcctgcgc gagcggcgcc tacgccatga tcgaagcccg
     2521 gcggatcctc ttggcgggcg aggccgacgt gatgttcgcg ggcggcgccg acgcggccat
     2581 aactcccgtc atgttccagg ggctgaacgc gatgcacgcg ttgtcgaagc ataacgatgt
     2641 gccggccgag gcgagtcggc cgttcgacgc ggatcgcgac gggttcgtct tcggcgaggg
     2701 tgccgtcgtc ttgacgctcg agactctggc gcatgcgaag gcacgcggcg cgcgtgtcta
     2761 cgccgaggtt ttgggcggtg ccctcacgag cgacgcgttc aacgtggtgg cgccggagcc
     2821 gaccgcgaag tatgcgacgg aggcggtcgt caaggggctg cgcaaggcgg ggctccaccc
     2881 ctctgacatc gatctggtgt gcgcgcacgg cacctcgacc cgcgcgaacg accgcaccga
     2941 gacggcggcg atccgccagg gcctcggcga ggcggcctac gacgtgcgag tcacggcgcc
     3001 caagtcaatg accgggcacc tgatcggtgc ggcgggcgcc ctggctggca tgctgtgcgc
     3061 gctgtcgatc acgaagagcg tgatcccgcc gacgatcaac tacgagaccc ccgacccgga
     3121 ttgcgacctc gactacacgc ccggcttcgc gcgtgaggcg agcgtgcgcc acgcgatcac
     3181 caacgcgttc ggcttcggcg gccagaactg cgtcgtcgcc ttcggcgccg tgtgatgccg
     3241 cattgcggtc tgcccggcgc cccggtgccg ggcgggaccc gcataagccc gttcccggcc
     3301 gcctccgggt gaggatcctg gccctgcata tggccgggag tgggtgttcg gggtcgtcag
     3361 gcgcgggtgt tcgcacgccg gcgtgggcgt tcggtcgccg gtcgccgact gtgggcgtct
     3421 gggcgcagat ggccgggtga agcgtcgggg ttgcgagccg ccctgctctg ggcaggcgca
     3481 gtgtcggcgg gtacggtggg ggaccaagac gccggtgcgc agtgccgccc gcctggcagc
     3541 gcggcgcggg gtaccggccg ctgggcgggc cctggcgtgt agtggcgcag accgggaggt
     3601 gcggcgatgg atgtggcgtc tggcgaccgg atcgtgattc gcggcaaggc ggtcggcaac
     3661 cccgaccgtc gcggtacggt gctcgaggtg cgcggcgacg agggccaagg gccgtacgtc
     3721 atccgtttcg acgacggccg cgaatcgctc gtctatccgg gccccgactt cgtcgtcact
     3781 cctgagtagt ggcgacccgg tagaggaacg cggccattgc ggcgcgagag atcgccttgg
     3841 ccggccggta cgagccgtcc gcgtatccgt cggtgacgtg ttgttgccgc agccacgcga
     3901 tggatccgca gaacgggctc gccgtaggca cgtcggcgaa tggttccgcg gtgcacgccg
     3961 gggcgtgggt ttcggaggtc tgggatcggt agagaaacgc ggccatcgcg gcgcgggaga
     4021 tcggcgcggc cggctgaaag gttccgttcg cgaagccggt ggtgatgccg ttctctttca
     4081 gccacgtgat cggcccgcag aacgggctcg tcgtgggcac gtcggagaac gggcgaaccg
     4141 tgcagcggat cgtcgcgccc tgcccgtcgt ctgcccggcg gtagaggaac gcggccatcg
     4201 cggcgcggga gatcggcgcg gccgggtgaa aggcgccatt tttgaagccg gtggtgatgc
     4261 cgcttgcctt cagccatgcg atcggcccgc agaacgggcc ggtggtcgcg acatcggtga
     4321 agggccgcgt cgtgcatctg ccgtcctcgt cgacgatcgg cgctttgcca gaccggccga
     4381 cgagaggtga gacggtgaaa gagccgccgg cgaggcttga gggaaccgcg aggacgctgc
     4441 ccgtcttcgg gtcggccatc tcgatggaga tcgggtggaa gctttcggcc ggtgccgtgg
     4501 cggcgctctt tcgagcgctg ccgtgcccgc cggcttctct cgtcgcggtc gctgaggtga
     4561 gggtgatcgg gtcggagggc gccaggctgg tcgccgtaca gctggagagg tttgaggcag
     4621 aggcgcagat gctcggtctc tcgacgatcc attccgcgga ggcctgcgct gcggcgaagt
     4681 cggcgacggt cttcgtgaag ctccagccgg cgctcgtatc gcgcatcgtc aaggtccacc
     4741 ggttgccggc gacgcggacg gcggcggtga cggtgtctcc cggccgtacc gcgtctgcgg
     4801 cgggcagggc gaccgatgcg ccgccgttct ccgcctggtc gccgtacatc tcccaccagg
     4861 cgttgtagtg cgcggcgccg tcgtgacaga aggcgttcac gccggcctgc tcgacgctgc
     4921 cgttgccgta gccgtcgatc ccgacccatt gcgccgagta cgaggtcgtc gtgccgcagg
     4981 tgagtgtcgg cacctgccac gtgcctgtca cggccgtgaa gacgccgccg gtggcgacgt
     5041 agccggccca gttcggcgtt cgcgccggcg ccaggtgccc ggcctgggcc tgggcgagcg
     5101 tcatcggccc cgccgcccgg ctcccggccg gagccggcac cagcctggcg gaaggggcgc
     5161 tttcggggga agtcgccggc acggtcacga tgggcgtcgc ggatgcggtc tgtgcgtcga
     5221 gcgtgacagg ggcgttcgac gtggcccgcg tgccggctgc gacggaatcg gtagggaggg
     5281 cacgggcact gcggctggtc ggggtgccgc cgatacccgg cgtcatcggc gcgggcggcg
     5341 tcatcgccgt gagaagcgcg ccggccgcga gtagcccgcc gaagagcagg acccaggtgc
     5401 ggcgtcgatc gtggtttgtc gcgtgagcgt gcatgcccgg cgtcccttcg gccttgtggg
     5461 ccggagggtg tgtcgtcggt gccggccttc agtggccgtt atcggacgtt tgcgcccgtc
     5521 gggcaggccg acgcgcctgg taaagcgcag acaaaccatg cccgcgcacc gaacattttt
     5581 gacgccacgt gagtggcggc tcatggttca gatgcggggg cgggttattt gcgcagggcg
     5641 aagccgccgc cccacccgat gacctcttgg gcggcgaggg tgatctgcag gaacccgaat
     5701 gcctcgagct cccgcgcgcg cttcagcgac ccgaccgagg ctgcggcaac gcccccggca
     5761 gtgacgagat cgacgagctg ctgcttcgcg tcggcgtcgt cgccggccac cagcacggtc
     5821 gcaggaacct ccccgacctg gcctgaggcg agggcgccgg cgaagtttgt attgaatgcc
     5881 ttgagaacct tcgcgcccgg caccaacgcc tgcagctctg ccgcagccga ggaatccgcc
     5941 ggcacggtca gcccgtcaaa ggtggtcggg tcgaccgggt tcgtgacgtc taccacgact
     6001 ttcccgtcca gttgggcgcc gtacgcctgt gccagctcag cgagcgccgc ataggggacg
     6061 gcaaggacga cgacctctcc cgtgatcggg tcgccgacgc tccccgctgt tccgccgacc
     6121 tgtgcggcca gggcggaggc cttctcctgg tcccgtccca agacctgtac ggaggcgcct
     6181 ccctttgctg tgatggcggc gaccgcagat ccgacgttgc ctgttccgat gacggtgaca
     6241 tcgctcatgg ttcttctctc cttggagtat gtgacggcaa tacgtttgcc tgggcaacaa
     6301 cactagcatg attagtttcc tgggcaacaa cccaggctat gatggaggta tgcagcagaa
     6361 ggtgccccgt atggatgcca ccgaggcccg ggcgtgggtg gcactggtgt ctgttgcgga
     6421 gttgcttccc gctactcttg atcagcagtt gaccgatgac atcggcctga tcaatttcga
     6481 gtacggcatt ctgagcatgc tcaatgtcgc cgacggtcag acgctccgta tgggcgagct
     6541 cgccgcggcg gtgaagtcgc cggcgccccg gctgtccaaa gcggtcaccc ggctcgaacg
     6601 aaaagggttc gtcgaacgcg cttcctgcgc cggcgatggc cgtgccatca acgttcgact
     6661 cacccgcgat ggccgtcgcg tctggctcag cgcaacgccg ccccatatct cactcgcccg
     6721 cgacacgatc cttggcgatc tcacccccga cgagctcacc cgcctcgccg aactcctcga
     6781 ggtgatcaag gcgcgtctgg acccagacgc gagtctcggc cgcatcccgg agcgcgctgc
     6841 gcccacccac caacctgacc actcgctgta agaggtcaat ccggaagggg tgaagagccc
     6901 gcgaaaaccg cggcacgtca ggggaggtga agagtggctc cgaacggcat cgatccgttg
     6961 acctttcgaa cttcagtcga acgcttatcg gacgtttgcg cccgtcgggc aggccgacgc
     7021 gcctgacaac gcgcagacaa accatgcccg cgcgccg
//
LOCUS       DASTNL010000101         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1558204, whole genome shotgun sequence.
ACCESSION   DASTNL010000101 DASTNL010000000
VERSION     DASTNL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558204"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1162)
                     /locus_tag="VFQ96_03250"
     CDS             complement(101..1162)
                     /locus_tag="VFQ96_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0256836.1"
                     /translation="MTRTLAIAAAVGLALTTAACSNGGSASDTGATGGSAESARTPTT
                     LTVYTDQHMPLVEALTGAYTKKTGVTFKLQGDATVGQIQAEGKASPADVFLSEDPGPV
                     AQLGKAGLVTPISGTTMRQVRPGLSSEKSLWVAYAARARVLYYNPKLITKSALPSTLA
                     AITDPQYKGSFAYAPSGAFAATTQYLISTIGEARTEKFLKAVKANGINEQKNGNVRDT
                     VEAGKHAMGLSNHYYWWVKAAEVGGPDHMTSKIYHFTAGDPGNLVLASGAAILKSSGQ
                     HKAAAAFVAWLTSADGGQQILANGDTDTAGAQYPVAPGLASSVVGSLDDVQGPAYDPD
                     IFADQQAAVDLLQKLGMTS"
     gene            complement(1378..2370)
                     /locus_tag="VFQ96_03255"
     CDS             complement(1378..2370)
                     /locus_tag="VFQ96_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEU0256837.1"
                     /translation="MHTDLPLWFETGSLVVLVLILVADLFLVAYRPHTPSVVECVLWI
                     AFYVALALGFALAITLLGDAPRAGEFVAGWLTEYSLSIDNLFVFVLIMARFAVPQNTQ
                     QEVLMVGIVIALVLRGIFIVIGAGLIANFSWIFYLFGAFLLYTAARQVMPNGGDDATG
                     QSENALTRFLRRRLRVSDQYEGFRLRTTVAGRRMLTPVLLVLVTLGGTDLLFALDSIP
                     AIFGITTSPFIVFTANIFALMGLRQLYFLLGHLIDRLEYLKYGIAVILAFIGVKLVLH
                     ALNTNELPFIAGGKGVSWAPEIGTGASLAVIVGAMAAATGASLIRLRWKARRGA"
     gene            2807..>3251
                     /locus_tag="VFQ96_03260"
     CDS             2807..>3251
                     /locus_tag="VFQ96_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0256838.1"
                     /translation="MVIDTDDGCRGEVGEILTRLGLDVIEAVSTNEALEAVCHARPHI
                     VVIDADIPEAPRSAEYGSGRRGGVETVRRIRSANDCLIMALSVHAEESDVVEGLDAGA
                     DDYVAKPFFRGVFQARVEALLRRPGIGTGLPAGGRHRKKMWPATAM"
BASE COUNT          507 a         1019 c         1161 g          564 t
ORIGIN      
        1 actgaccgac cggccggccg gagggcgcgc gacccggccg gtcagcggga acgtggggcg
       61 cctacctgaa ggccggccgg ccgggcccgt gctcgaaagc tcagctcgtc atgccgagct
      121 tctgcagaag gtcgaccgcg gcctgctggt cggcgaagat atccgggtcg taggcgggcc
      181 cctgcacgtc gtcgaggctg cccaccacgg agctggccag gcccggcgcc accgggtatt
      241 gcgcgccggc ggtgtctgtg tcgccgttcg ccaagatctg ctggcccccg tcggcggagg
      301 tgagccaggc gacgaatgcc gcagcggcct tgtgctggcc gctcgacttt agaatcgccg
      361 cgccggaggc gagcaccagg ttgcccgggt cgccggccgt gaagtggtag atcttcgacg
      421 tcatgtggtc gggcccgccg acctccgccg ccttgaccca ccagtagtag tggttcgaca
      481 ggcccatcgc gtgcttgcct gcctctaccg tgtcgcgcac gttgccgttc ttttgctcgt
      541 tgatgccgtt cgccttcacg gccttcaaga atttctcggt tctcgcctcg ccgatcgtgg
      601 agatcaggta ttgggtggtc gccgcgaatg ctccggaggg ggcgtaggcg aaggagccct
      661 tgtattgcgg gtcggtgatg gcggccagag tcgagggcaa cgcgcttttt gtgatgagct
      721 tcgggttgta gtagagcacg cgtgcgcggg cggcgtaggc gacccacagg ctcttctccg
      781 agctgagccc cggccgaacc tgccgcatcg tcgtgcccga gatcggggtg acgaggcccg
      841 ccttgccgag ctgggcgacg gggcccgggt cttcggagag gaacacgtcg gccggtgagg
      901 ccttgccttc tgcctggatc tgcccgaccg tcgcgtcgcc ttgcagcttg aacgtgacgc
      961 ccgtcttctt cgtgtaggcg ccggtcaacg cctcgacgag cggcatgtgc tggtcagtgt
     1021 aaacggtcag cgtcgtgggt gtgcgggcgc tctcggcgga gcctcccgtg gcacctgtgt
     1081 cgcttgcgga cccgccgttc gagcaggcgg ccgtcgtcag ggcgagcccg acggcggcgg
     1141 cgatcgcgag tgttcgggtc atgcgaggca ggcgcatggg ggtcctttcc gtgggatttc
     1201 tggggtgggt gcgggccggc cttatcccgg cgcgggcgaa gaggtcgcgg cccgctcttg
     1261 gggcggccgg atcggcatgc cgcgccgtct aagcacacgc ggctaggaga tagcttatag
     1321 tgaggtaagg ctaacctcaa cacttcggat tgctgcgttg ggttttatgc gccgcgctca
     1381 tgcgccgcgc cgcgccttcc atcgcagcct gatgaggctc gcgcctgtcg ccgccgccat
     1441 cgcgccgacg atgacggcca gcgaggcgcc cgtgccgatc tccggtgccc acgagacgcc
     1501 cttgccgccc gcgatgaacg gcagctcgtt ggtgttcagg gcgtgcaaga cgagcttcac
     1561 gccgatgaac gccaagatca cggcgatgcc gtatttcagg tattcgagcc ggtcgatcaa
     1621 gtgcccgagc aagaaataga gctgccgcag acccatcaag gcgaagatgt tcgcggtgaa
     1681 cacgatgaac gggcttgtcg tgatgccgaa gatcgcgggg atggagtcga gggcgaagag
     1741 taggtcggtg ccacccagcg tcacgagcac gagcaatacg ggggtgagca tccgccggcc
     1801 ggccaccgtg gtgcgcagcc ggaagccctc atactgatcg ctcacccgca gccggcgccg
     1861 caagaagcgc gtcagagcgt tctcgctctg ccccgtcgca tcgtcgccgc cgttcggcat
     1921 gacctgccgg gcggcggtat agagcaggaa cgcgccgaac aggtagaaga tccagctgaa
     1981 gttcgcgatc aagccggcgc cgatcacgat gaagatgccg cgcagcacga gtgctatgac
     2041 gatgccgacc atcagcacct cttgctgggt gttctgcggc accgcgaacc gcgccatgat
     2101 gagcacgaag acgaacaggt tgtcgatgct gaggctgtat tccgtcagcc agccggcgac
     2161 gaactcgccg gcgcgcgggg cgtcgccgag cagggtgatc gccagcgcga acccgagcgc
     2221 cagggccacg tagaacgcga tccagagcac gcattccacg acgctcggcg tgtgggggcg
     2281 gtatgcgacg aggaacagat cggccacgag gatcagcacg agaacgacga gggaaccggt
     2341 ctcgaaccag aggggcaggt cggtgtgcat gagggccttt cacggtgggc gctgggcgtc
     2401 ggtgcgtgaa agtctctccc gcatccactc gggcgccgca tggcgcttgc cgtcggatgc
     2461 cgccgccccg gggccttacg gcccgtcttg acgaggtcgg tgcggcaggc cctgtgcggc
     2521 ccgcctggga tactccctct ctgtcgacca ccctaccgtc gtgcgccgac ggtttccgtg
     2581 cgggtgccgc cgattccctg tctcagggga ggaggcaccc ggcggtcgtc gtgaggctcg
     2641 tgaacccggc cgattccgtg tctcggggag ggcgacgatt ctcgcctgat cctcaaggtt
     2701 cataaagatg ccaaagtttg tgcaaatttc gaggcttcgt cgtttcgtcg cagaggccgg
     2761 ggggagcaca atttcggttg tggacccgac acccaggctg acctctgtgg tcattgacac
     2821 cgacgacggc tgtcgaggcg aggtgggaga gattctcacc cggttgggcc tcgacgtgat
     2881 cgaggcggtg tcgaccaatg aggccctcga ggcggtgtgt cacgcccggc ctcacatcgt
     2941 cgtgatcgac gcggatattc ccgaggcgcc acggtcggcg gagtacgggt cgggccggcg
     3001 agggggagtc gagacggtgc ggcgcatccg ctcggcgaac gattgcctca tcatggcgct
     3061 cagcgttcac gcggaggagt cggatgtggt cgaagggctc gacgcgggcg ccgacgatta
     3121 cgtcgccaag ccgttctttc gcggcgtctt ccaggcgcgt gtcgaagcct tactgcgccg
     3181 gcccggcatc ggcacggggc tgcccgccgg cggccggcat cgaaagaaga tgtggccggc
     3241 gactgcgatg a
//
LOCUS       DASTNL010000102         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_77031, whole genome shotgun sequence.
ACCESSION   DASTNL010000102 DASTNL010000000
VERSION     DASTNL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77031"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1540
                     /gene="ugpC"
                     /locus_tag="VFQ96_03265"
     CDS             443..1540
                     /gene="ugpC"
                     /locus_tag="VFQ96_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU0256839.1"
                     /translation="MASVNYDKATRVYPGTSRPAVDQLDLQIADGEFLVLVGPSGCGK
                     STSLRMLAGLEEVNEGDIFIGERNVTDVPPKDRDIAMVFQNYALYPHMTVAENMGFAL
                     KIAGVNKDERAQRVLEAAKLLDLEPYLGRKPKALSGGQRQRVAMGRAIVRSPKVFLMD
                     EPLSNLDAKLRVQTRTQIASLTRRLGVTTVYVTHDQTEALTMGDRIAVLKDGVLQQVG
                     TPRDLYADPENVFVAGFIGSPAMNLHTADLTPEGVKFGSSVVPLNRETLAAASGHNAT
                     VGIRPEDVIVSPTRAEGLELHVDLVEELGADGYLYGHHDVSGTRSDFVARVDGRQHPN
                     AGDTVYITPKLGHLHVFDATTGVRLGGAVVD"
     gene            1600..>2513
                     /locus_tag="VFQ96_03270"
     CDS             1600..>2513
                     /locus_tag="VFQ96_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEU0256840.1"
                     /translation="MSGSLTITSAVADPALLDLPWNIPLDDWTKEHIALLPKGISRHL
                     VRFAKLSGAVIAIKETTVEMARREYDMLRTLLKLDVPCVEPVAVITNRTDDNGTPLKP
                     VLVTRHLKFSLPYRALFSQTLRPDTATRLVDALAALLARLHVAGFFWGDVSLSNTLFR
                     RDAGAFAAYLVDAETGQLYDTGLSNGQRENDLEIARVNIAGELMDLAAGGRVADELDP
                     VSVSEGIVAAYHSLWTELTARESFPSSERWRIADRVDRLNRLGFDIEELSIKTDDRGT
                     TVRIQPKVVDAGHHQRRLLRLTGLDVQE"
BASE COUNT          443 a          895 c          778 g          397 t
ORIGIN      
        1 gtcgtgctcg aacagccggc gaggcccaca gtggcaagcc ccaacagcag ggccgcggcc
       61 gtcgcgtgcc gcatcctcat cgtgccgtca tctcttctcc ctcggtatcc ccatgattcc
      121 catgctttgt atccccgtga cctcgcggcc ctcgcggccc ggcgccgatc cggccgcccg
      181 caaggcctcg ggtggggcac gcggccgacg agaacttagc acagcctaac ctcaccgtaa
      241 tgggcgaacg ccgggaaaat tccgtgcggt gcgtcgccac gcgcccgcga cccccgagcc
      301 ggccgcgggc gtgcggcgat cgggcgtggc atgaccggat ttaaaaagtc gtagcatggg
      361 ggtcacgccg cagccgcggc ctctacccgt tcactcggat cgtccggcac gtacctgccg
      421 gtgaaggagt tctgaaagca tcatggctag cgtcaactat gacaaggcga ctcgggtcta
      481 cccgggaacg agtcggcccg ccgtcgacca gctcgacctg cagatcgccg atggcgagtt
      541 tctcgttttg gtcggcccct ccggctgcgg caagtccacc tctctgcgca tgctcgccgg
      601 cctcgaagag gtgaacgaag gcgacatctt catcggcgag cgcaatgtca ccgacgtgcc
      661 gccgaaagac cgcgacatcg cgatggtctt tcagaactac gcgctgtatc cccacatgac
      721 ggtggccgag aacatgggct tcgccctgaa gatcgccggc gtcaacaaag acgagcgggc
      781 acagcgggtt ctcgaggccg cgaagctgct cgacctcgaa ccgtacctcg gccgcaagcc
      841 caaggctctc tccggcggcc agcgtcagcg cgtcgccatg ggccgcgcga tcgtgcgcag
      901 cccgaaggtg ttcctcatgg acgagccgct ctcgaacctc gatgcgaagc tgcgcgtaca
      961 gacccgcacg cagattgcct cgctgacccg gcgtctcggc gtcaccacgg tgtacgtcac
     1021 gcacgatcag accgaggcgc tgacgatggg cgaccgcatc gcggtcttga aagacggggt
     1081 gctgcagcag gtgggcacgc cccgcgacct gtacgccgac ccagagaacg tcttcgtcgc
     1141 cggcttcatc ggcagccctg ccatgaatct gcacacggcc gacctcaccc cggaaggcgt
     1201 gaagttcggc agcagcgtcg tgccgctgaa ccgtgagacc ctggccgcgg ccagcgggca
     1261 caacgcgacg gtcggcatcc ggccggaaga cgtcatcgtc tccccgaccc gggccgaggg
     1321 gctcgagttg catgtcgacc tcgtcgaaga gctcggcgcc gacggctatc tgtatggcca
     1381 ccacgacgtg agcggcacac gttccgactt cgtcgcacgc gtcgacggcc gccagcaccc
     1441 gaacgcgggc gacacggtct acatcacgcc gaagctcggc cacctgcacg tcttcgacgc
     1501 aacaaccggg gttcgcctcg gcggtgccgt cgtcgactag gaacgaacga gagacgagag
     1561 ggtgccggcg ccttcaggcg ccggcaccgt cgtggcatta tgagcggatc actgaccatc
     1621 acgtcggcgg tcgccgaccc ggcgctactc gacctgccgt ggaacatccc attggatgac
     1681 tggacgaagg aacacatcgc ccttcttccg aagggcatct cccgccatct ggtacgcttc
     1741 gccaagctct ccggcgcggt catcgccatc aaagagacca cggtcgagat ggcgcggcgc
     1801 gaatacgaca tgctgcgcac cctcctgaag ctcgacgtgc cgtgcgtcga acccgtcgcc
     1861 gtcatcacga accgaaccga cgacaacggc acgccgttga agcccgtcct cgtcacgcgg
     1921 catctgaagt tctcgttgcc gtaccgcgcg ctcttctcac aaacgctgcg gcccgacacg
     1981 gccacgcgcc tcgtcgacgc cttggccgct cttctggcca ggctccacgt cgccggcttc
     2041 ttctggggcg acgtctccct ctcgaacacc cttttccggc gcgatgccgg cgccttcgcc
     2101 gcctatctcg ttgacgcgga gacggggcag ctgtacgaca ccggcctctc gaacgggcaa
     2161 cgcgagaacg atctggagat cgcccgcgtg aacatcgccg gcgaactcat ggacctcgcc
     2221 gcgggcgggc gcgtcgcgga cgagcttgac ccggtctccg tctcggaggg tatcgtcgcc
     2281 gcctaccact cactctggac ggaactcacc gcccgggagt cgttcccgag ctcggaacgg
     2341 tggcgcatcg ccgaccgcgt cgaccggctg aaccggttgg gcttcgacat cgaggagctc
     2401 tcgatcaaga ccgacgaccg aggcacgacg gtgcgcatcc agccgaaggt cgtcgatgcg
     2461 gggcaccatc aacggcgcct tttacgactc actggcctcg acgtgcaaga gaa
//
LOCUS       DASTNL010000103         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_634185, whole genome shotgun sequence.
ACCESSION   DASTNL010000103 DASTNL010000000
VERSION     DASTNL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634185"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFQ96_03275"
     CDS             <1..799
                     /locus_tag="VFQ96_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256841.1"
                     /translation="LLGLQGLLIFVVNLLAGQSSGGTMSVSNKVINDIAYGHVNDVVA
                     WIALVVIIALYAIFTIVRDRHRRSRNLVAQPVGLAVVKIVFLAAAGAALVLACSAGSG
                     VPWAVPIVLAIVFGWTFLLAQTRFGRYVYAIGGNAEAARRAGINLSRIRLIAFALASL
                     TAGAAGIFYAARLGSISNNINGGQYVLLAVAAAVIGGTSLFGGRGKIVHALLGGLIVA
                     TIYNGMGLLNLSAATQLMVTALVLLAAVTVDALSRRNRATANASATM"
     gene            complement(1002..1877)
                     /locus_tag="VFQ96_03280"
     CDS             complement(1002..1877)
                     /locus_tag="VFQ96_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0256842.1"
                     /translation="MTRFIAIIVEDDDEISGVLSTALGRLGMETIEVRTGAEAVEAAR
                     QNEPTIVTIDVGLPGMDGFEVARRIRAFSSCYVIMVTARGEEIDVLEGLGAGADDYIV
                     KPVRIRELRARVDAMLRRPRTGGTVATATPRIGHETVAPRSAEGTEQAVRREPAAQRQ
                     QVAPVSMIRPAAPADDELETRLHHGDLLLDMRCRIVELDGNPVELTRYEFDLLTTLME
                     SGRRVCSKASLALALRSNGRGDLGGEYVSDADLRTIDTHIANLRRKLGDDYRDPRVIE
                     TVRGVGYRMAAAAAA"
     gene            2132..2284
                     /locus_tag="VFQ96_03285"
     CDS             2132..2284
                     /locus_tag="VFQ96_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256843.1"
                     /translation="MNRVMAKRRANAEASSSESIHRWEDEGGALPDGEQAERDAAAKA
                     ADATEE"
BASE COUNT          358 a          824 c          841 g          418 t
ORIGIN      
        1 cctgctcggc ctgcagggcc ttctgatctt cgtcgtcaac cttctggcag gccagtcgag
       61 cggtggcacg atgtcggtga gcaacaaggt catcaatgac atcgcctacg ggcacgtcaa
      121 cgacgtggtc gcctggatcg cgctcgtcgt gatcatcgcc ctctacgcga tcttcacgat
      181 cgtgcgcgac cggcaccggc gcagccgcaa cctcgtcgcc cagccggtcg gcctcgccgt
      241 cgtgaagatc gtcttcctcg ccgcggcagg tgccgcgctc gtcttggcgt gcagcgccgg
      301 cagcggcgtg ccgtgggcgg tgccgatcgt cttggcgatc gtgttcggct ggaccttctt
      361 gctcgcgcag actcggttcg gccggtacgt gtatgcgatc ggcggcaacg ccgaggcggc
      421 ccggcgcgcg ggcatcaacc tgtcgcgcat ccgcctgatc gcgttcgccc tggcgagcct
      481 gacggccggc gcagcgggca tcttctacgc cgcacggctc ggttcgatct cgaacaacat
      541 caacggcggc caatacgtgc tgctggcggt cgcggccgcc gtgatcggcg gaacgagcct
      601 gttcggcggg cgcggcaaga tcgtgcacgc cctcttgggc ggcttgatcg tcgcgacgat
      661 ctacaacggc atgggccttc tgaacctgtc ggcggcaacc cagctcatgg ttaccgcgct
      721 cgtgctgctc gccgcggtca cggtggatgc gttgagccgg cgcaaccgcg ccacggcgaa
      781 cgccagcgcc accatgtgac gaacccggcg cccgtcgtgt ggcccgccgc cctcctcgtg
      841 gcgcgcgggc cgcacggcca tgtggccggc gtcacgtctg gctgcgggct cggtcaatgg
      901 ccgagtgtgg ccgggcgatc ctggccggcc acgcccgcca gggcgggctc atcggccggg
      961 tctggccggc ctctcccgag cccggccttc ggcctggccg gtcaggccgc tgccgcggcg
     1021 gccatgcggt agccgacgcc acgcacggtc tcgatgaccc gcgggtcgcg gtagtcgtcg
     1081 ccgagctttc tgcgcaggtt cgcgatatgc gtgtcgatgg tgcgcaggtc ggcgtcgctg
     1141 acgtattcgc cgcccagatc gccccggccg ttgctgcgca gagcgagggc gagggaggcc
     1201 ttgctgcata cccggcggcc ggattccatg agggtggtaa gcaagtcgaa ttcgtagcgc
     1261 gtcagctcaa cggggttgcc gtcgagctcg acgatgcggc agcgcatgtc gagcagtagg
     1321 tcgccgtgat gcagccgcgt ctccagctcg tcgtcggcgg gcgccgcagg ccggatcatc
     1381 gagaccggtg cgacctgctg tcgctgggcg gcgggctcgc gcctgacggc ctgctccgtg
     1441 ccttcggccg agcgcggcgc caccgtctcg tggccgatgc gcggtgtcgc ggtcgcgacc
     1501 gtaccgcccg tgcggggtct gcgcagcatc gcgtcgacgc gggcccgtag ctcgcggatg
     1561 cgcaccggtt tgacaatgta gtcgtcggcg ccggcgccga gcccttcgag cacgtcgatc
     1621 tcttcaccgc gcgccgtcac catgatgacg tagcaggagc tgaacgcccg aatgcgccgg
     1681 gcgacttcga agccgtccat gccgggcagg ccgacgtcga tggtgacgat ggtcggctcg
     1741 ttttgccggg ccgcctcaac ggcttcggca ccggtgcgca cttcgatcgt ctccatgccg
     1801 agccgaccca gtgcggtgga aagcacaccg cttatctcgt catcgtcttc gacgataatc
     1861 gcgatgaacc gggtcatacc tagaaaggta gccgcaggat tggccgtaac aaagccctca
     1921 caatgctttt aattgcggct ttgcacacaa ttgaggccgt cttggcacac cctaaaggca
     1981 taccgacgcc gaccggccgc gacacaccgc ggccttctct gaaggctagc cagtgtcgat
     2041 gcgtttgcat agcatgcggt gccgccgatg accctcggcc gggttccgcg aacccctggc
     2101 gggctcgccg gggtcgtctt acagtgcggg tatgaatagg gtcatggcga agaggcgggc
     2161 caacgccgag gcctcctcat cggaatcgat ccaccgttgg gaagacgagg gcggtgccct
     2221 gcccgacggt gagcaggcgg aacgagacgc ggcggcgaag gccgcagacg cgaccgaaga
     2281 gtgaaacggg agaagaaagg gagggccgca cgctgaacgc gtgcggccct ccccctttct
     2341 tggccttggc taccgaatca cttcgaccga tagaggaagg ctgcgaccgc ctcgcgggtg
     2401 atctgcacgg tcggcttgaa caggccgccc ttgtacccgg t
//
LOCUS       DASTNL010000104         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1191251, whole genome shotgun sequence.
ACCESSION   DASTNL010000104 DASTNL010000000
VERSION     DASTNL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191251"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFQ96_03290"
     CDS             complement(<1..732)
                     /locus_tag="VFQ96_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0256844.1"
                     /translation="MTGCTAGQGGKNAPATPSASAATSAPSQAASDGACPSVPAGKAS
                     GSVKVAGAFLKTPKVTFTKPLKASGFQRSYVIKGTGTVTAASDTVDIALAAYNGVTGK
                     ALSVQGFGSSPVLVTAIQSVVPGVQNAVDCVPVGSRVVLTSTVSQAFGGVTAPSQWKL
                     KKTDSIVFVADVTKILPQRADGVVQHDLPKNMPTVAFAPSGQPKVTLPKSAPPTKTTI
                     VDLRKGDGATVQSGDLVTVQYQGVNW"
     gene            complement(898..1368)
                     /locus_tag="VFQ96_03295"
     CDS             complement(898..1368)
                     /locus_tag="VFQ96_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0256845.1"
                     /translation="MAEYDYRVTVAWTGDRGSGTSGWRDYGRQSLVAAEGRPPIEASA
                     DRVFHGDAERWNPELLLLAALSQCHMLSYLRQAALAGVLVVGYRDEAAGRIAVQADGS
                     GRFVGATLHPQVTLAEGSMLPAARRLHEPAARQCFIAASVDFPVHREPSFRVRE"
     gene            complement(1373..>3804)
                     /locus_tag="VFQ96_03300"
     CDS             complement(1373..>3804)
                     /locus_tag="VFQ96_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEU0256846.1"
                     /translation="ALPGIGVAGPPALAWSVTRGYLVQLLDRLPPGHVALCLPAAQGI
                     PGLPHCTTRTAEYRIEVLDAAGQAASGLSGAARAAGAARGTIRVVVAAQLERIPPTID
                     TVIQVLAPGVADIVQGRLALRRIAVEHVSRAQFERFAAELRRHADLLGLTSGAPAGPV
                     LLEDLSDDSDSRAGRGGLPCPVGLRDGVVCRVDLVRDGPHAVVGGTTGSGKSELLTTW
                     VAAMARAASPREVAFLLIDFKGGAAFQALRTLPHCVGVITDLDEAGARRAVTSLAAEV
                     RRRERLLAAAGATDVSDPACATRLGRLVIVVDEFQSLLRALPDLDAVFTDIAARGRSL
                     GMHLILCTQRPAGVVREALLANCGLRLCLRVHAKADSEAVIGDGRAALLPVDAPGRCL
                     VGQVGAAGEVQIALTSNDLIDGIRERYQAAPAPDRPWLDPLPDSIDLQALQGLASSST
                     RGMPAAASGVFGFRPWLGMVDDPAGQRQYPARYDAAAGGALLCVGDAGSGKTTLLESL
                     AAQGEGSAAGARILRGVEECWDAVQDAAEQCLRPGGAILPPLLLDDLDALLARLGDDY
                     AHEFAEALGLLLREGGRRVAVVAACRRLAGPMNALADLFTERLLLRIGDRADYLVAGG
                     EAGLHDARLPAGGGTWHGRRIQIATASPADASRADAGGTGRAGEVTPGAAAAQLVPVE
                     VAEGALIVSRRPGSLAERLRAGGVPVVELDPGRRPEPSEGTTPGAEERPCLVADPESW
                     QSAWALFHAYRARLPVVFEACTPVDVRMLTRRRTLPPVLEPYRGHVWVLDTRDRLRRG
                     ILVTPGTAAAR"
BASE COUNT          570 a         1321 c         1399 g          514 t
ORIGIN      
        1 ccagttcacg ccctgatact gcacggtgac gaggtcgccg ctttgcacgg ttgcgccgtc
       61 tcccttgcgc aggtcgacga tcgtcgtctt cgtcggcggc gccgatttcg gcagcgtgac
      121 cttcggttgg ccggagggcg cgaacgccac ggtcggcatg ttcttcggca ggtcgtgctg
      181 caccacgcca tctgcacgtt gcggcaggat cttcgtcaca tcagccacga agacgatcga
      241 gtcggtcttt ttcagcttcc actggctggg cgccgtgaca ccgccgaacg cctgtgacac
      301 cgtcgaggtg agcacgaccc gggagccgac cggaacgcag tcgacggcat tctgcacgcc
      361 gggcacgaca ctctggatgg ccgtgacgag cacgggcgag ctgccgaagc cctgcaccga
      421 gagagccttg ccggtcacgc cgttgtaggc ggcgagtgcg atgtcgacgg tgtcggatgc
      481 ggcggtgacc gtaccggtgc ccttgatcac gtagctgcgc tgaaagcccg aggccttcag
      541 aggcttcgtg aacgtcacct tcggcgtctt caagaaagcc ccggccactt tcaccgagcc
      601 ggacgcctta ccggcgggaa cgctcgggca ggctccgtcg ctcgcggcct ggctgggggc
      661 ggacgtcgcg gcagaggccg agggcgtcgc cggggcgttc ttgccgccct gcccggctgt
      721 gcatccggtc atgagaagga gaacgcctac ggctgcggtg agggcgaggg ttttacgcac
      781 ggaggaggac ttccctgatc gaggtctgat gggccactcc accgtacacg ccggatctga
      841 ccgtctcctc caacagccct aaggcgggac gccggctcgc cggcacccgc ccgctcctca
      901 ctcccgcacg cggaaggagg gctcccggtg cacgggaaag tcgacggagg cggcgatgaa
      961 gcattgccgg gccgcgggtt cgtgcagccg ccgcgcggcc gggagcatcg atccctcggc
     1021 gagggtgacc tgcgggtgca gagtcgcgcc gacgaacctg ccggaaccgt ctgcctgtac
     1081 ggcgattcgg cctgccgcct cgtcacggta accgacgacg aggacgccgg cgagggcggc
     1141 ctgacgcaga tagctcagca tgtggcactg gctaagagcc gccaaaagca gcagttcggg
     1201 gttccaacgc tcggcgtcgc cgtggaagac gcgatccgcg gaggcctcga tcggtggtcg
     1261 gccttcggcc gccacgaggc tctgccgccc gtagtcgcgc caaccgctcg tgccgctccc
     1321 gcgatcgccc gtccacgcca cggtgacccg atagtcgtat tccgccaccg tctcatcttg
     1381 ccgcggccgt gccgggtgtg acgaggatgc cgcgccgcag gcggtcgcgg gtgtcgagca
     1441 cccagacgtg gccccggtag ggttcaagca cgggtggcag ggtacgccgg cgggtgagca
     1501 tgcgcacgtc cacgggcgtg caggcctcga agacgaccgg cagccgtgcc cggtaagcat
     1561 gaaacagggc ccacgcggat tgccacgact ccgggtcggc gacgaggcaa ggccgttctt
     1621 cggcgccggg cgtcgtaccc tccgaaggtt cgggccgacg gcccggatcg agctcgacga
     1681 cgggaacgcc gcccgcgcgg aggcgctcgg cgagagagcc gggccgtcgg gagacgataa
     1741 gcgccccttc tgccacctcc accggcacca gctgcgccgc ggcggcgccc ggcgtcacct
     1801 cgcccgccct gcccgtgccg ccggcatccg cccggctcgc atcggccggg gatgcagtgg
     1861 cgatctggat gcggcggccg tgccacgtgc cgccgccggc cggcagccgc gcgtcgtgga
     1921 gcccggcctc gccgccagcc acgaggtagt cggcgcgatc gccgatccgc agcaagaggc
     1981 gctcggtgaa caggtcggcc aaggcgttca tcggccccgc caggcgccgg caggccgcga
     2041 cgacggcgac ccgccggccg ccctcccgca gaaggagacc gagcgcttcg gcgaactcgt
     2101 gggcgtagtc gtcgcccagc cgagcaagga gcgcgtcgag gtcgtcgagg agcaggggcg
     2161 ggaggatggc accgcccggg cgcaggcact gttcggcggc gtcctgtacc gcatcccagc
     2221 attcttcgac gccccgcagg attctcgcgc ccgccgccga cccctcgcct tgcgccgcca
     2281 ggctctcgag cagcgtcgtc ttgccactgc ccgcgtcgcc gacgcacagc aacgcgccgc
     2341 ccgcggccgc gtcgtatcgc gcggggtact gccgctggcc cgccgggtcg tcgaccatgc
     2401 cgagccacgg acgaaacccg aagacgcccg aggcggcggc cggcatgccg cgcgtcgatg
     2461 aggaggcgag gccttgcagg gcctggaggt cgatgctgtc gggcagcggg tcgagccacg
     2521 gcctgtcggg ggcgggggca gcctggtacc gctcgcggat gccgtcgatg agatcgttcg
     2581 aggtgagggc gatctgcact tcgccggccg ccccgacctg gccgacgagg cagcggccgg
     2641 gcgcatcgac gggcaggagg gcggccctgc cgtcgccgat caccgcctcg ctgtcggcct
     2701 tggcgtgcac gcgcaggcag aggcgcagac cgcagttggc aaggagtgcc tcgcgaacga
     2761 cgccggcagg ccgctgggtg cacaggatca ggtgcatgcc gagcgagcgg ccgcgagcgg
     2821 cgatgtcggt gaaaaccgcg tcaaggtcgg gcaaggcccg caagagcgat tggaactcat
     2881 cgaccacgat gacaagccgc ccgagccttg tggcacacgc ggggtcggag acgtcggtgg
     2941 cgccggccgc cgccagaagg cgttctcggc gccgtacctc ggcggcaagg ctcgtgacgg
     3001 cgcgtcgggc gcccgcctcg tcgaggtcgg tgatgacgcc gacgcagtgc ggcaacgttc
     3061 gtaaggcctg gaacgcggcc ccgcccttga agtcgatgag gaggaaggcg acttcgcgcg
     3121 gcgaggcggc gcgcgccatg gccgcgaccc atgtggtgag cagctcgctt ttgccgctgc
     3181 ccgtggtgcc gcccacgacg gcatgcgggc cgtctcgtac gagatcaacc cggcagacga
     3241 cgccatctct cagcccgacc gggcaaggaa ggccgccgcg ccccgcgcga gagtcggaat
     3301 cgtcggaaag gtcttcgagg agcaccgggc cggccggcgc gccggaggtg aggccgagga
     3361 ggtcggcgtg ccggcgcagt tcggccgcga accgttcgaa ctgggcgcgg ctgacatgct
     3421 cgacggcgat gcggcggagt gcaaggcggc cctgcacgat gtcggcgacg cccggagcga
     3481 gaacctgtat gacggtgtcg atcgtcggcg ggatacgttc cagctgcgct gcgacgacga
     3541 cacggatcgt gccgcgggcc gcacctgccg cacgggctgc acccgaaagc ccgctggccg
     3601 cctgcccggc cgcgtcgagg acctcgatac ggtattcggc cgttcgtgtg gtgcaatgcg
     3661 gcagccccgg aatcccctgc gccgcgggca ggcacagggc cacgtgtccc ggtggcaggc
     3721 ggtcgagaag ttgcaccagg tagccgcggg tgacggacca tgcgagggcc ggtgggcccg
     3781 ccacaccgat gcccggcagc gcgg
//
LOCUS       DASTNL010000105         5024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1004597, whole genome shotgun sequence.
ACCESSION   DASTNL010000105 DASTNL010000000
VERSION     DASTNL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004597"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFQ96_03305"
     CDS             <1..547
                     /locus_tag="VFQ96_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU0256847.1"
                     /translation="MQQLRNMGSIKKMIAMLPGAGAMRQQLDNFDEREIVRTEAIIQS
                     MTHAERVNPKVLNGSRRLRVARGSGMTVTDVNQLVTRFEQAAKMMKTVAKGGVPNIPG
                     MGPVVGAGRASTRRAQKKHKGSRSGNPAKRAAENAAASVRGTGAGPAEPTPSGAGFGL
                     GPAGAAGRDAIDPIVKKMLRH"
     gene            722..1129
                     /gene="rpsP"
                     /locus_tag="VFQ96_03310"
     CDS             722..1129
                     /gene="rpsP"
                     /locus_tag="VFQ96_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU0256848.1"
                     /translation="MAVKIRLKRIGKIRAPHYRIVVADAHTKRGGRVIEEIGRYVPTA
                     DPSIIEVDSPRAQYWLGVGAQPTEPVAAILKLTGDWGTFKGEKGAKNTVRPQEPKPVF
                     VADENKKPVLVPKSEAPAAPAAEETAAAPAEGE"
     gene            1142..1378
                     /locus_tag="VFQ96_03315"
     CDS             1142..1378
                     /locus_tag="VFQ96_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019127083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU0256849.1"
                     /translation="MLEDALTHLVKGIVGHPDDVTVTARQSHRGDVLEVRVDGTDLGR
                     VIGRGGRTAKALRAVMGALADGSRVLVDIVDTDR"
     gene            1375..1998
                     /gene="rimM"
                     /locus_tag="VFQ96_03320"
     CDS             1375..1998
                     /gene="rimM"
                     /locus_tag="VFQ96_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171094.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU0256850.1"
                     /translation="MSAPRPGAGRRQLRVGRLVKAHGLKGGLRLELYTDDPARRFVPG
                     ASFALQVPAGSRWHGKSVTLAELRSFNGHAVAFFDGVTDREAAQSLVRAILWIDADPA
                     EESGEPNAWYDHQLVGLTAWKDGEPVGKVVRVDHLPAQDLLAVAAGGREILVPFVKEI
                     VPALDIARGTVVLTPPPGLFEDLPDDGSLPDGSPPGDEVTPRPPASS"
     gene            complement(1970..2359)
                     /locus_tag="VFQ96_03325"
     CDS             complement(1970..2359)
                     /locus_tag="VFQ96_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEU0256851.1"
                     /translation="MSILGLLPLIIMVVALVDLITRPDADVRHMPKLVWLLIVVFMPL
                     IGGVLWFTMGRRYGQARPAASHPAGRGRQKTQRVTTAARPAPAAAPRRSTEEQLADLE
                     REIKYYEAQDRIRQLEDEIRKKREGEA"
     gene            2472..3395
                     /gene="trmD"
                     /locus_tag="VFQ96_03330"
     CDS             2472..3395
                     /gene="trmD"
                     /locus_tag="VFQ96_03330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU0256852.1"
                     /translation="MRIDVVTIFPDYFAPLDVSLIGKARAGGLLDVRLHDLRDFTHDR
                     HHTVDDTPAGGGAGMVMRPEPWGEAFDRLLGEAPPTSTGDVTADRADPGTRAACDAGA
                     GRSGTEPAGPVIVFPSPAGHLFTQRTAHRLAARERLVFGCGRYEGIDQRVYEEAARHA
                     PVELLSLGDYVLNGGEVAVLAMLEAIVRLVPGVVGNPESLVEESHEDGLLEAPVYTKP
                     STWRGLSVPPVLLSGDHARVDAWRRQQQRERTGRVRPDLLRVTLPASPARECGPSSVA
                     ANAANAASGVGQAPPERAVPAARFGRPPQTL"
     gene            3516..3866
                     /gene="rplS"
                     /locus_tag="VFQ96_03335"
     CDS             3516..3866
                     /gene="rplS"
                     /locus_tag="VFQ96_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU0256853.1"
                     /translation="MSNLLDQVNAASLKTDIPDFRPGDTVKVHVNIVEGSRSRVQIFQ
                     GVVIARQGSGVHETFTVRKVSFQVGVERMFPVHSPIIDHIEIVTRGDVRRAKLYYLRA
                     LRGKKAKIREKREA"
     gene            3967..4710
                     /gene="lepB"
                     /locus_tag="VFQ96_03340"
     CDS             3967..4710
                     /gene="lepB"
                     /locus_tag="VFQ96_03340"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0256854.1"
                     /translation="MTSDVAQEAPAGRRRHRGGRYGAGKFVRDVIVIILIAILASYLL
                     KTFVIRSFYIPSGSMENTLEINDRIIVDELVPRFFPIRRGDVVVFTDPGGWLPPSPPE
                     HRNAVATGVDWFLSQVGLSASDSDDHLVKRVIGIGGDHVVCCNASGQITVNGHALKEP
                     YTVIPAGHTAAATLHFNVRVPKGELWVMGDNRYNSQDSSRNQNLPGKGFVPVSDVVGR
                     AVVISWPVSRWTWLSRYPQVFAGSSAGGA"
     gene            4758..>5024
                     /locus_tag="VFQ96_03345"
     CDS             4758..>5024
                     /locus_tag="VFQ96_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU0256855.1"
                     /translation="MTVERELFRLGNAAVLGIDEVGRGALAGPVAVGVVAVGRAPGPM
                     PEGLRDSKLLSAARRALLAPACAAWAGLHGVGMATAAEIDRLGIV"
BASE COUNT          778 a         1743 c         1762 g          741 t
ORIGIN      
        1 gatgcagcag ttgcgcaata tgggctcgat caagaagatg atcgcgatgc tccccggcgc
       61 cggggcgatg cgtcagcagc tcgacaattt cgacgagcgg gagatcgtgc gcaccgaggc
      121 gatcatccaa tcgatgacac acgccgagcg agtcaacccg aaggtcttga acgggtcgcg
      181 ccggctgcgt gtcgcccggg gcagcggcat gaccgtcacc gacgtcaacc agttggtcac
      241 ccggttcgag caggccgcga agatgatgaa gacggtcgcc aagggtggcg tgccgaacat
      301 cccggggatg gggcctgtcg tgggggcggg gcgcgcctca acgcggcgcg cgcagaagaa
      361 gcacaagggc tcgcgctccg gaaaccctgc caaacgggcc gcagagaacg cggcggcgag
      421 tgtgcgtggc actggggccg ggccggccga gcccacaccg agcggcgcgg gcttcggcct
      481 gggccctgcc ggcgccgcgg gaagagacgc gatcgacccg atcgtgaaga agatgctgcg
      541 acactgaacg cccgccggga cccccgccgg ttggcataaa gcgggccggg tctgccagaa
      601 tagaggcgga cgtcggcccg accctctatc cggccgtcgt gccgtgacca gacctcctgc
      661 cccgcgcagc cccatgcccg cggcagtcag ttcgacccac caccaagtca tcaggagaat
      721 tgtggccgtt aagatccgcc tcaagcgcat cggcaagatc cgtgccccgc actaccgcat
      781 cgtcgtcgcc gatgcgcaca ccaagcgcgg cggccgggtc atcgaagaga tcggccgtta
      841 cgtgccgacc gccgacccct ccatcatcga ggtcgactcg ccgcgcgccc agtactggct
      901 gggcgtgggc gcgcagccca ccgagccggt cgcggccatc ctgaagctga cgggtgactg
      961 gggcaccttc aagggcgaga aaggtgcgaa gaacacggtg cggccgcagg agccgaagcc
     1021 ggtgttcgtc gccgacgaga acaagaagcc cgtcctcgtg ccgaagagcg aggcgcccgc
     1081 cgcgccggcc gcggaagaga ctgccgccgc gcccgctgag ggcgagtagg cggacccggt
     1141 cgtgctggaa gacgctctca ctcaccttgt gaagggcatc gtcggccacc ccgacgacgt
     1201 gaccgtcact gcgcgacagt ctcatcgggg ggatgtcctt gaggtgcggg tggatggcac
     1261 cgacctcggc cgggtcatcg gccgcggcgg ccgcaccgcc aaggcgctgc gtgcggtgat
     1321 gggagccctc gcagacggca gccgcgtctt ggtcgacatc gtcgacaccg accgttgagc
     1381 gcgccccgcc cgggtgcagg ccgccgtcag ttgcgggtcg gcaggctcgt gaaggcgcac
     1441 ggtttgaagg gtgggctgag gctcgagctt tacaccgacg atcccgcccg ccggttcgtg
     1501 ccgggcgcct ccttcgcctt gcaggtgccg gcaggctcgc gatggcacgg caagtcggtc
     1561 acgctggcag agcttcgctc cttcaacggc cacgcggtgg ctttcttcga tggggtgacc
     1621 gaccgtgagg cggcgcagag ccttgtgcgg gccatcctct ggatcgatgc cgacccggcg
     1681 gaagagagcg gcgagccgaa cgcctggtac gaccaccaac tcgtcggcct gacggcctgg
     1741 aaagacggcg agcctgtcgg taaggtcgtg cgtgtcgacc acttgccggc gcaagacctc
     1801 ctcgccgtcg cggcgggggg ccgtgagatt ctcgtgccgt tcgtcaaaga gatcgtgccg
     1861 gcgctcgaca ttgcccgcgg caccgtggtg ctgacccctc cgccgggcct cttcgaggac
     1921 ctgcccgacg acgggtcgct gcccgacggg tcgccgccgg gcgacgaggt tacgcctcgc
     1981 cctcccgctt cttcctgatc tcgtcttcga gctgccggat gcggtcttgt gcctcgtagt
     2041 acttgatctc gcgttccagg tctgccagct gctcttccgt actgcgccgc ggcgcggcgg
     2101 ccggcgccgg cctcgccgcc gtcgtgaccc gctgggtctt ctgccggccg cgccctgccg
     2161 ggtggctcgc cgccggccgc gcctgcccgt aacgccgccc catggtgaac cacaagacgc
     2221 cgccgatgag gggcatgaag acgacgatca gcagccagac cagcttcggc atgtgccgca
     2281 cgtcggcgtc gggcctcgtg atgagatcga cgagggccac caccatgatg atgagcggaa
     2341 gcaggccgag aatcgacatg cctcaagctt aggcgaaggc cccgaacggc ccctgagacc
     2401 ggccgaccga ggcgccgcct cgcgggcctg gcgctccgag cgctccggcg ctgcctgcct
     2461 acaatcggga aatgcgcatc gacgtggtca cgatctttcc cgactacttc gcgcccctcg
     2521 acgtctccct catcggcaag gcccgagcag gtgggctcct cgacgtgcgg ctccacgacc
     2581 ttcgtgactt cacccatgac cggcaccaca ccgtcgacga cacgccggcg ggcggcgggg
     2641 cgggcatggt catgcgaccc gagccttggg gggaggcctt cgaccggctc ctgggagagg
     2701 ccccgccgac ttctaccggc gacgtcaccg ccgaccgggc ggacccgggg acgcgagccg
     2761 cttgcgacgc gggcgccggc cggtcgggta cggagcccgc gggccccgtc atcgtcttcc
     2821 cctcgcccgc cgggcacctc ttcacgcagc gcacagcgca ccggcttgcc gcgcgcgagc
     2881 gcctcgtctt cggctgcggc cgctacgagg gcatcgacca gcgggtctac gaagaggccg
     2941 cccggcacgc tcctgtcgag ctcttgagcc tcggcgacta cgtgctgaac ggcggcgagg
     3001 tcgccgtgct cgcgatgctg gaggccatcg tgcggctcgt ccccggcgtc gtcggcaacc
     3061 cggagagcct cgtcgaggaa agccacgaag acggcctgct cgaggcgccg gtctacacga
     3121 agccgtcgac ctggcgcggg ctgtcggtgc cgcccgtgct gttgagcggc gaccatgccc
     3181 gggtcgacgc gtggcgccgt cagcagcagc gggagcgtac cgggcgagtc aggcccgacc
     3241 tgctgcgcgt cacgcttcct gccagccctg ccagagagtg cggaccatcc tccgtcgcgg
     3301 cgaacgcggc gaacgcggcg tcgggcgtgg ggcaggcgcc gccggagcgg gccgtgccgg
     3361 cggcgcggtt tggccgcccc ccgcagacgt tatagagtga gacgctgtgc cttgcgctcc
     3421 tctgccacgg gggaggatgc ggtctctcgg cacgacacgc ctttcttgac accccatgtg
     3481 cggccgaccc gtggcgaccg cagagagcga ccatcatgag caacctcctc gatcaggtca
     3541 acgctgccag cctcaagacc gacatccccg acttccggcc cggtgacacg gtgaaggtgc
     3601 acgtcaacat cgtggaaggc agccgttcgc gcgtgcagat cttccaaggg gtcgtgatcg
     3661 cgcggcaggg ctcgggtgtg catgagacct tcacggtgcg caaggtcagt tttcaggtcg
     3721 gcgtcgagcg gatgttcccc gtccactcgc cgatcatcga ccacatcgag atcgtcacgc
     3781 gcggcgacgt gcgccgtgcc aagctctact acctgcgcgc gctgcgcggc aagaaggcga
     3841 agattcgcga gaagcgcgag gcgtaatatc cgtcgaggct cggcgaggta ggggccgccc
     3901 gggcggagca tcctcaaaac cgaccgtgcc taggatgagc cgagatggtg acgggcgggc
     3961 gatggaatga cgagtgacgt ggcgcaggag gcgccggcag gccgtcgcag gcatcgtggc
     4021 gggcggtacg gcgccggcaa gttcgtccgc gacgtgatcg tcatcatcct catcgcgatc
     4081 ctggcctcgt acctcttgaa gaccttcgtc atcaggtcgt tctacatccc ttccggctcg
     4141 atggagaaca ccctcgagat caacgaccgc atcatcgtcg acgagctggt gccccgcttc
     4201 ttccccatcc ggcgcggcga tgtcgtcgtg ttcaccgacc ccggcggctg gctgccgccg
     4261 agcccgcccg agcatcggaa cgcggtagcg acgggcgtcg actggttcct ctcgcaggtc
     4321 gggctttctg cctccgacag cgacgaccat cttgtgaaac gcgtcatcgg catcggcggc
     4381 gaccacgtcg tgtgctgcaa cgccagcggc cagatcacgg tgaacggtca cgccttgaaa
     4441 gagccgtaca cggtgatccc cgccgggcac accgccgcgg cgacgctgca tttcaacgtg
     4501 cgggtgccga agggcgaact ctgggtgatg ggcgacaacc ggtataactc gcaagactcc
     4561 agccgcaacc agaacctgcc cggcaagggc ttcgtgcccg tcagcgacgt ggtgggccgc
     4621 gcggttgtca tcagctggcc ggtgagccga tggacctggc tcagccgcta cccgcaggtc
     4681 ttcgccggct cctccgccgg cggcgcgtga cggcgggccg ggggaggcct gtgcgaccgg
     4741 gtaccggatg gcccgacatg acggtggagc gagagctctt ccgcttgggc aacgcggccg
     4801 tcctcggcat cgacgaggtg ggccgaggcg ccctcgcggg cccggtcgcc gtcggcgtgg
     4861 tcgcggtcgg ccgggccccg ggccccatgc ccgagggact gcgagactcg aaactgctga
     4921 gcgctgcccg gcgtgccctg ctggcgccgg cctgcgcggc ctgggcgggg ctgcacggcg
     4981 tcggcatggc gacggcggcc gagatcgacc ggctgggcat cgtc
//
LOCUS       DASTNL010000106         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1005800, whole genome shotgun sequence.
ACCESSION   DASTNL010000106 DASTNL010000000
VERSION     DASTNL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005800"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFQ96_03350"
     CDS             complement(<1..744)
                     /locus_tag="VFQ96_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256856.1"
                     /translation="MAHHARTIHPRASRVRRWLSVIGAVALAAALLAIADAPAAAEGP
                     APAALYYASPDGSDDPGATCGQVDDPCSLARALDVLVPGTVSTIYLVPARDGGSVFTT
                     DVGWEVSTAGDVAMEPLGAGTATLDGQGVAPHILEVFGGAGNSVNLRLRRIEISGSQV
                     RSYDLGESSGGGIHVHDGASLLASDCIFRNNAVRALVAGSGSAISYGGAILNDGGLVG
                     VEGCLFVGNDVTATAISGSGLAWGGSITNI"
     gene            1168..1428
                     /locus_tag="VFQ96_03355"
     CDS             1168..1428
                     /locus_tag="VFQ96_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU0256857.1"
                     /translation="MPTIVVEVMPKAEILDPQGKAIAGALERTGRGTFAVRAGKRFEL
                     TVPGPVDAALLDSVRQIAHDVLSNDVIEDVVGVRVEPEAGQA"
     gene            1425..2123
                     /gene="purQ"
                     /locus_tag="VFQ96_03360"
     CDS             1425..2123
                     /gene="purQ"
                     /locus_tag="VFQ96_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU0256858.1"
                     /translation="MTRIGVVTFPGSLDDRDARRAVRAAGAEDVPLWHGDRGLAGVDA
                     IVLPGGFSYGDYLRCGAIASLSPIMREVIDAAGRGMPVLGICNGFQMLTEAHLLMGGL
                     VRNDHGRFICRAQRLRVENTSTAWTNAFETGEEIVIPLKNGEGSFVASPDDLARIEGE
                     GLAVFRYAGVNPNGSLGDIAGVTNERGNVVGLMPHPEHATEPGFGPDTTAAMRSGVDG
                     LRFFTSALHSLLAA"
     gene            2165..>2693
                     /locus_tag="VFQ96_03365"
     CDS             2165..>2693
                     /locus_tag="VFQ96_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256859.1"
                     /translation="MTAQPHGPGTTPRGVGGAAPAATQALAALPLAAILVAAVAMRPH
                     LGTTIASHWSGGAHPDGFSPTWGSFWVTVLVTAGLLVFSTVASTFARRASWSRVWPAI
                     ATTVGGMLACSWILSAWATAAAPSPAEATLGPRLVLLLPALACGAGIYLLLPPTPAPH
                     TAGARRPVRQVQPGEA"
BASE COUNT          438 a          947 c          909 g          399 t
ORIGIN      
        1 gatattcgtg atggagcctc cccatgcgag gcccgaaccg ctaatggcag tcgcagtcac
       61 atcattccca acgaacaaac acccctcaac gccgaccaac ccgccgtcat tcaggatggc
      121 tccaccgtac gagatggccg agccactccc tgctaccaag gccctcacag cgttgttccg
      181 aaagatgcaa tccgacgcga gcaaagaagc accatcatgg acatgaatcc caccaccact
      241 agattcgcca aggtcgtatg acctgacctg ggaaccactt atttctatgc gtctcagtcg
      301 caaattgaca ctgtttcctg caccgccgaa gacctcgagg atgtgaggcg ccacgccttg
      361 tccatcgagg gtcgcagtgc cggcgccgag gggctccatg gccacgtcgc cggcggtgga
      421 gacctcccag ccgacatcgg tagtgaagac cgagccgccg tcgcgcgccg gcaccaggta
      481 aatggtgctg acagtgcccg gcacgagcac gtcgagcgcc cgcgcgaggc tgcacgggtc
      541 gtccacctgc ccgcaggtcg cgcccgggtc atcactgccg tcgggggagg cgtagtacag
      601 cgcggcgggt gccgggccct ccgccgccgc aggcgcgtcg gcgatggcaa ggagcgcggc
      661 cgccagcgcc actgcgccga tgacgctcag ccagcgccgc acccgcgagg ctcgtgggtg
      721 gatggttcga gcgtggtggg ccataggagc tcctgccgtc gagacgcgcg tcgggatctg
      781 tcgcgctatg tgccgagcgc gtcggtgcgc cgcggcgaga tgtcgtgaca tggtgccaag
      841 cgggcactcc gacggtgcgg ggcccacgtt ctgcgcccac agtatcgctg tgagattact
      901 atgtacagac cctgtagatt atttacgatt catgagagtc ccatctacgg cgaaccagag
      961 gacggcggca agcggagggc cacctccgcc acggccgcca aatccgtgac aagtcggggc
     1021 aggctcccgg cagcgcaccc atcctctcgt cggcggcgca cgacggtcaa cgcgcggcct
     1081 atgtcgcaag ccgccgggga ggggtgccct agaatcattg cgtccaggtt gggcgaggcg
     1141 acagacgaga cgacgacaag gagcacggtg ccgacaatcg ttgtggaggt catgcccaaa
     1201 gcggagattc tcgaccctca gggcaaggcg atcgcgggcg ccctggaacg caccggccgg
     1261 ggcaccttcg ccgtgcgagc cggcaagcgg ttcgagttga cggtgccggg ccccgtcgat
     1321 gcggcattac tcgactccgt gcgccagatc gcgcacgacg tgctctcgaa cgacgtcatc
     1381 gaagacgtcg tcggcgtccg cgtggaaccc gaggcggggc aggcgtgaca cggatcggcg
     1441 tcgtgacctt tcccggctcc ctcgacgacc gggacgcccg ccgcgcggtg cgcgccgccg
     1501 gggccgagga tgtgccactc tggcacggcg accggggcct ggcgggagtc gacgcgatcg
     1561 tactacccgg cgggttcagc tacggcgact acctgcgctg cggtgcgatc gccagcctct
     1621 cgccgatcat gcgtgaggtc atcgacgcgg caggccgcgg catgcccgtg ctcggcatct
     1681 gcaacggatt ccagatgctc accgaggcgc acctcttgat gggcggcctg gtgcgcaacg
     1741 accacggccg attcatctgc cgggcgcaac gcctgcgggt ggagaacacg tcgaccgcgt
     1801 ggaccaacgc cttcgagacg ggcgaggaga tcgtcatccc cctcaagaac ggggagggct
     1861 ccttcgtcgc ctcgcccgac gacctggcgc ggatcgaggg tgagggtctc gccgtgttcc
     1921 gttacgccgg ggtgaacccc aacggatcgc tcggcgacat cgccggggtg acgaacgagc
     1981 gcggcaacgt cgtcggcctc atgccgcacc ccgagcacgc cacggagccc gggttcggcc
     2041 ccgacacgac ggccgcgatg cgcagcggcg tcgacgggct tcgatttttc acctcggcac
     2101 tgcactctct gctcgccgcc taggggcgcg gcggggccga cttaccacaa gacggaggca
     2161 ggccatgacg gcgcagccac acgggccagg cacgactcct cggggcgtgg gaggcgcggc
     2221 gccggccgcc acccaggcgc tcgcagcgtt gcctctcgcc gcgatcctcg tcgcagccgt
     2281 ggccatgagg ccccacctgg gcacgacgat cgcctcgcac tggtcgggcg gcgcccaccc
     2341 cgacgggttc tcccccacct ggggctcgtt ctgggtcacc gtgctcgtca ccgccgggct
     2401 gctcgtattc tcgaccgtgg cgagcacctt cgcacgccgg gcctcatggt ccagagtgtg
     2461 gccggcgatc gcgacgacgg tcggcgggat gctcgcctgc tcgtggatcc tttctgcctg
     2521 ggccactgcg gccgcgccca gcccggcgga ggcgacgctc ggcccgcgtc tcgtcctgct
     2581 cctgccggcg ctggcctgcg gcgccggcat ctacctgctt ctacccccga cacccgcgcc
     2641 gcacacggca ggtgcgaggc ggccggtacg acaagtccag cccggggaag cgg
//
LOCUS       DASTNL010000107         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_637355, whole genome shotgun sequence.
ACCESSION   DASTNL010000107 DASTNL010000000
VERSION     DASTNL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637355"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..440
                     /gene="ribH"
                     /locus_tag="VFQ96_03370"
     CDS             9..440
                     /gene="ribH"
                     /locus_tag="VFQ96_03370"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0256860.1"
                     /translation="MNVVIVAGTWHKEIADGLVAGARRALDAAGAQHRLVQVPGSFEL
                     PLASKAALTAGADAVVALGVIVRGETPHFDFVASAATEGLTRVALDTGRPVGFGVLTC
                     DTEQQGLDRAGLPGSKEDKGAQAAEAALRMVQVLRGLGPRP"
     gene            complement(594..2471)
                     /locus_tag="VFQ96_03375"
     CDS             complement(594..2471)
                     /locus_tag="VFQ96_03375"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEU0256861.1"
                     /translation="MTITTNPRGASGVAQPGRATTDPTLRAWVEKVASVTQPDRVEWC
                     DGSLAEFDRLTRLLVAEGKLLRVNPEWRPNSFLARSDPDDVARVEARTFICSRAEADA
                     GPTNNWCDPDEMRATLDALLPGSMRGRVMYVMPFSMGPLGGAISQLGVQVTDSAYVVI
                     SMHAMARMGTAALELIEAGQSWVPAIHTVGMPLVDDEGRRIDDVAWPCNTTKKYIVHF
                     PETREIVSYGSGYGGNSLLAKKAFALRIASVMGRDEGWLAEHMLLIRVTSPEGRSYHV
                     AAAFPSACGKTNMAMMQPTLPGWSVETIGDDIAWLRPGADGRLWAINPENGFFGVAPG
                     TGESTNPTAVDTLWGNTIFTNVALQDDGDVWWEGLTDEPPAHLIDWRGDDWTPAVGRP
                     AAHPNSRFTVAAAQCPSIAEGWDAPDGVPLDAIIFGGRRSTNVPLVTEADTWEQGVFM
                     GATIASEKTAAAEGTVGELRRDPFAMLPFCGYNMADYWRHWLQVGAGLDRKAPRVFRV
                     NWFRKGDDGSYLWPGFGENSRVLAWIVRRIEGSAEAEETPVGLVPARPDSAAPDAEGG
                     FDVTGLGLAGEALAELFAVHAEPWLAECDLTEEYFARFGGKVPPALVAELASMRYKLA
                     R"
     gene            2554..>3688
                     /locus_tag="VFQ96_03380"
     CDS             2554..>3688
                     /locus_tag="VFQ96_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256862.1"
                     /translation="MAPTKHPAPEAAREKRPAREPQRDEAAGSPAPDLATLGRRIRHL
                     RTTRGLTLGALGRAVDAAPSLLSLVENGKREPRLSLLQRLAETFEVPMRELLNDEAPD
                     RRSAIEIELLRLQKNPTYLSLGLPQIKPTKGTPTRTLEALVGLYRELAKRDREFVATP
                     EEARRANTEQRMRMRAVNNYLPAIETLAEERVKAAGHTVGALTHREVGIMAEQLGFSL
                     IYVTDLPSSTRSVTDLANKRIYLPPASIPGGHGLRSMALQAIAHRLLGHERPANYAEF
                     LQQRLEITYFAACCLMPRGAAVAFLQNAKKEHNLAVEDFRDAFGTTHESAAMRLTNLA
                     TSHLDMTLHFVRVGGDGALMKGYENDGLNLPTDVTGAIEGQFVC"
BASE COUNT          559 a         1296 c         1286 g          547 t
ORIGIN      
        1 gccgggggct gaacgttgtc atcgtcgccg gaacctggca caaggaaatc gccgacggtc
       61 tcgtcgcagg cgcccggcgg gcgctcgacg cggcgggcgc ccagcaccgg ctcgtgcaag
      121 tgccgggcag cttcgaactg ccgctagcct cgaaggcggc gctcacggcc ggcgccgatg
      181 ccgtcgtcgc cttgggcgtg atcgtgcgcg gagagacccc gcatttcgac ttcgtcgcct
      241 ccgccgccac cgaggggctc acgcgtgtcg cgctcgacac cggcaggccg gtcgggttcg
      301 gcgtgctcac ctgcgacacg gaacagcagg gcctcgaccg ggctggcctc cccggctcga
      361 aagaggacaa gggcgcgcag gccgccgaag ccgcactacg aatggtgcag gtgttgcgcg
      421 gcctcggccc gaggccttga gcctaggtct gcctggaatc cgggcctgtc cggaacccgg
      481 gcctgtccgg aaccgggtca ggcagagcga gatcatgcca ggccggctca tgctcgagac
      541 gaggccccgc cgggtgccag gcccaagggg gtaggcccgg ccgtcgtggg gcttcagcgg
      601 gcgagcttgt aacgcatgct ggcgagctcg gcgacgagcg ccggcggcac cttgcccccg
      661 aagcgggcga agtactcctc ggtcagatcg cattctgcga gccacggttc ggcatgcacg
      721 gcgaagagct ccgccagcgc ctcccccgca agccccaggc cggtcacgtc gaagccgcct
      781 tcggcgtcgg gcgcggccga gtctggccgg gctggcacga ggccgaccgg ggtctcttct
      841 gcctccgccg agccttcgat acgccgcacg atccaggcga gcacccgtga attctcgccg
      901 aagcccggcc acaggtagga accgtcgtcg cccttgcgga accagttgac gcggaagacg
      961 cggggggcct tacggtcgag gccggcgccg acctgcagcc agtgccgcca atagtcggcc
     1021 atgttgtagc cgcagaacgg cagcatcgcg aacgggtcgc ggcgcagctc gccgacggtg
     1081 ccctccgccg cggcggtctt ctccgaggct atcgtcgccc ccatgaagac gccctgctcc
     1141 cacgtgtcgg cctcggtgac gagcggcacg ttcgtggagc ggcgcccgcc gaagatgatc
     1201 gcgtcgagcg gcacgccgtc gggcgcgtcc cacccttccg cgatcgacgg gcactgggca
     1261 gcggccacgg tgaaacggga gttcgggtgg gcggccggcc ggcccacggc cggagtccag
     1321 tcgtcgccgc gccagtcgat caggtgcgcc ggcggctcgt cggtgagccc ctcccaccac
     1381 acgtcgccgt cgtcttgcag ggcgacgttc gtgaagatgg tgttgcccca cagggtgtcg
     1441 acggcggtcg ggttggtgga ttcccccgtg ccgggcgcca cgccgaagaa gccgttctcg
     1501 gggttgatgg cccagagcct gccgtcggcg ccgggccgca gccatgcaat gtcgtcgccg
     1561 atcgtctcga cgctccaccc tggcagcgtg ggctgcatca tcgccatgtt cgtcttgccg
     1621 caggccgagg ggaacgcggc ggcgacatgg tagctgcgac cctcgggcga ggtgacgcgg
     1681 atcaagagca tgtgctccgc cagccaccct tcgtcgcggc ccatgacgct cgcgatgcgt
     1741 aacgcgaacg ccttcttcgc caagagggag ttgccgccgt agccggagcc gtacgagacg
     1801 atctcgcgcg tctcgggaaa gtgcacgatg tatttcttgg tcgtgttgca cggccaggcg
     1861 acgtcgtcga tgcgccggcc ctcgtcgtcg acgagcggca tgccgaccgt gtggatcgcc
     1921 ggcacccaac tctggcccgc ctcgatcagc tccagggccg cggtgcccat gcgtgccatc
     1981 gcgtgcatgc tgatgacgac gtaggcggag tcggtgacct gcacgccgag ctgcgagatc
     2041 gccccgccca aggggcccat ggagaacggc atcacataca tcacccggcc gcgcatcgac
     2101 ccgggcagca gggcgtcgag cgtggcccgc atctcgtcgg ggtcgcacca gttgttggtg
     2161 gggcccgcgt cggcctcggc gcggctgcag atgaacgtgc gcgcctcgac ccgggcgacg
     2221 tcgtcggggt cgctgcgggc gaggaagctg ttgggccgcc attcggggtt gactcgcagg
     2281 agcttgccct ccgccacgag cagacgcgtg agcctgtcga actcggccag cgagccgtcg
     2341 caccactcga cgcggtcggg ctgggtgacg gaggcgacct tctcgaccca ggcgcggagc
     2401 gtggggtcgg tggtcgcgcg gccgggctgg gcgacgcccg aggcaccgcg ggggttcgtc
     2461 gtgatcgtca tggctctagc ttcgggcgaa tcatgcgatg ttttacgcct cttcgcgcgt
     2521 gaagattcgt ggttctttct gtattgtcaa ggaatggccc cgacaaagca ccccgccccc
     2581 gaagccgcac gcgaaaaacg gccggcccga gagccgcagc gtgacgaggc ggcgggttcc
     2641 ccggcgcccg acctcgcgac gctcgggcga cgcatccggc acctgcgcac cacgcgcggc
     2701 ctgacgctcg gcgcgctcgg ccgcgcggtc gacgccgcgc cgagccttct ctccctcgtg
     2761 gagaacggca agcgggagcc tcgcctctcg cttctgcagc gcctcgccga gacgttcgag
     2821 gtgccgatgc gagagcttct caacgacgag gcgcccgacc ggcgcagcgc gatcgagatc
     2881 gagcttctgc gcttgcagaa gaaccccacc tacctgagcc tcgggctgcc gcagatcaag
     2941 ccgacgaagg gcacacccac ccgcacgcta gaggcgttgg tcggcctgta ccgcgagctg
     3001 gcgaaacgcg accgcgagtt cgtcgctacc ccggaggagg cacggcgggc gaacacggag
     3061 cagcgcatgc ggatgcgcgc ggtgaacaac tacctgcccg cgatcgagac gctcgccgag
     3121 gaacgggtga aggccgccgg ccacacggtg ggcgccttga cgcaccgcga ggtcggcatc
     3181 atggcggagc agctgggttt ctcgctcatc tacgtgaccg acctgccctc ttccacccgc
     3241 tcggtcaccg acctcgcgaa caaacgcatc tacctgccgc ccgcctccat tccgggcggg
     3301 cacggcctgc gttcgatggc gttgcaggcg atcgcccacc gcctgctcgg gcacgagcgg
     3361 cccgccaact acgccgagtt cttgcagcag cggctggaga tcacctactt cgccgcctgc
     3421 tgcctgatgc cgcgcggcgc cgccgtcgcc ttcttgcaga acgccaagaa ggagcacaac
     3481 ctcgctgtcg aagactttcg cgacgcgttc ggcacgactc acgagtcggc ggccatgcgc
     3541 ctgacgaacc tcgccacctc acacctcgac atgacgttgc atttcgtgcg ggtgggcggc
     3601 gacggggccc tcatgaaggg ttacgagaac gacgggctga acctgccgac ggatgtcacg
     3661 ggcgcaatcg agggccagtt cgtgtgcc
//
LOCUS       DASTNL010000108         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_638908, whole genome shotgun sequence.
ACCESSION   DASTNL010000108 DASTNL010000000
VERSION     DASTNL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638908"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFQ96_03385"
     CDS             <1..356
                     /locus_tag="VFQ96_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256863.1"
                     /translation="LEDSALTAALGRAAPGEAPAVEVARQRGVLTGVLADIVSTFSPS
                     IVVLGGFLTDVAPRDLDGYARRVCAEARPTVGRSLVVRLAELVENRLLYGAAEAAFDD
                     QVLAAEVLERWCADL"
     gene            complement(384..1421)
                     /locus_tag="VFQ96_03390"
     CDS             complement(384..1421)
                     /locus_tag="VFQ96_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0256864.1"
                     /translation="MSAKTDAPAEQKPRVTLSEIARHADVSVPTVSKVLNGHADVSAL
                     TRSKVEEALRAHQYRRRPRAQTSDLLELVFHELESEWAIEIIQGVERVAREGGLSVVV
                     TESGDRHSPSPGWIDGVLRRRPLGVILVFSGLSEENKRKLRSRRVNFVVVDPSGEPDP
                     DVASVGSANWAGGMSAVRHLTDLGHRRIGMITGPGDMLCSVARGDGYRAALSRAGIPF
                     DPDLVYDGTFHVQSGKDGAAYLLSLPEPPTAIFAGSDLQALGVYETARARNIRVPDDL
                     SVVGYDDLRLARWAGPPLTTVRQPLTEMAATAARLLVRLEEEPDLPLNQRIDLATSLV
                     VRQSTAAPAAR"
     gene            1991..3280
                     /locus_tag="VFQ96_03395"
     CDS             1991..3280
                     /locus_tag="VFQ96_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0256865.1"
                     /translation="MAATSLLAACTTGGGSGSGGATSAASGGGSSKPIKFWNMPWGGT
                     AFNPLDKKITLAYKPKKGLPAATYQEIQWANFTQTFSSAIASNTGPAVSSGGGTQAFQ
                     FAEQGKIAYADHLLDTWKKNGLYDDFLPGVLDSMKTSKGYAAVPYNLDDRVMWYNKAL
                     LQKAGVEPPTDWASYKSACAALKKIGVFGFGVGAGTGNLIGSHILTAFMINNGGGLFN
                     AKQEPAVVTDRNVEAIEYVLELIKSGYSDPRAATYTNDNVYAQWKANKFGMGWDTAGA
                     SVSVGGTVAAQMVVGDPLTGPHGTKGGLYFPNNIMMYTNTPSQEGSEAFMTYYYQHMK
                     ELWTKKTGIGLPPLKSIADTSAVSSDPNTSKIINVWQPILKTWAAPGGAALFYNVANT
                     VDGTQPMITFAQSIFGGKTDAKTALTTLSNSIEGLMK"
     gene            3374..4357
                     /locus_tag="VFQ96_03400"
     CDS             3374..4357
                     /locus_tag="VFQ96_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0256866.1"
                     /translation="MSVGSMRESLKMARRGVGAAGNGRVPKAGRAGNGRGKSKMARSR
                     ASTLLMFAIPSIILLVLLNLYPVVYAAEQSLRNGDLLSAGKFVGLHNYASMLDSPVFW
                     HAAWFTLIFTVTGVFGSWAIGLALALVLRTHIPAGGVFKVLLLLPWVVPIVVSATSWN
                     WLVATPQSPLPILAHALGLGNILFLANPVMAQVTVCVFKVWISFPFMMMMMASALESV
                     DVNVYEASKVDGATSWQTFRQITLPLVSRTTYISWILMTIFCVNDFPTIFLLTGGGPA
                     GATQSLVVLAYSTVFQNFQTGPGVAIAFMMTLVLVVISVVLYRQIRKANVA"
     gene            4354..>5230
                     /locus_tag="VFQ96_03405"
     CDS             4354..>5230
                     /locus_tag="VFQ96_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0256867.1"
                     /translation="MSVSASPVALAPAAPHKADGHHHAELGMRGRWWRFTLLVVITLI
                     VLVPVGVVVLLSVQPALGSTKTGWTLQNFATVFSQTSVGIWLRNSTIVTLVTVVVAVV
                     VAAPAGYVLSRGRSKLVSGYSLTLFVVQSLPVVTAIIPLFILFAKFGLVDNLAGITII
                     YVGSTISVAVWMMAAYYDSIPIDLEEASWIDGCSVLGSFGRIVLRNSLPGILSTAIFS
                     FLLAWNDYLVALVFLRSDHNFTLQIGMETFFQQNQTNWGLVMAVAVVMMLPPVIIFAV
                     LNRYFSVGGIGGSLAG"
BASE COUNT          848 a         1730 c         1695 g          957 t
ORIGIN      
        1 cactcgaaga ctccgcgctg acggcggcgc tcggccgcgc ggcccccggg gaggcgccgg
       61 cggtggaggt ggcgcgtcag aggggcgtgc tgacgggcgt gctcgccgac atcgtctcga
      121 ctttcagccc ctcgatcgtc gtcttgggcg ggttcttgac cgacgtcgcg ccccgtgacc
      181 tcgacgggta cgcccgccgg gtctgtgccg aggcccggcc cacggtcggg cgctcgctgg
      241 tcgtgcgcct ggccgagctc gtcgagaatc gcctgctgta cggcgcggcc gaggcggcct
      301 tcgacgatca agtgctcgcg gcagaggtgc tcgaaagatg gtgcgcagac ctctgacgcc
      361 gaagacggct tcggtgccgc gggctaccgg gctgccgggg ccgccgtgct ctgccgcaca
      421 acgagactcg tcgcgaggtc gatgcgctgg ttcaatggca ggtcgggttc ttcttcgagc
      481 cgcacgagca ggcgtgcggc cgtcgccgcc atctcggtca gcggctggcg caccgtggtc
      541 agcggcgggc cggcccagcg agccaggcga aggtcgtcgt aacccacgac cgagaggtcg
      601 tcgggcacgc gaatgtttct ggcccgcgcc gtctcgtaca cccccagggc ctgcaggtcg
      661 ctgccggcga agatcgccgt cggaggctcg ggcagcgaga gcaggtacgc ggcgccgtcc
      721 ttgccgctct gcacgtggaa cgttccgtcg tagacgaggt cggggtcgaa cgggattccc
      781 gcgcggctga gcgctgcacg gtagccgtcg ccgcgggcga ccgagcacag catgtcgccg
      841 gggccggtga tcatcccgat ccggcgatgg ccgaggtcgg tcaggtgccg caccgcagac
      901 atgccgccgg cccagttggc ggagcccacc gaggcgacgt cggggtcggg ctcgccggag
      961 gggtcgacga cgacgaagtt tacgcgacgg ctgcgcagct tgcgcttgtt ctcttcggag
     1021 aggcctgaga agaccaggat cacgccgagg gggcggcggc gcagcacccc gtcgatccag
     1081 cccggcgagg ggctgtgtcg gtcgccgctc tcggtgacga cgacgctgag cccgccctct
     1141 cgggccaccc gctccacgcc ctggatgatc tcgatcgccc actcgctctc cagctcgtgg
     1201 aagacgagtt cgagaaggtc ggaggtctgg gctcggggcc tgcgtcggta ctggtgggcc
     1261 cgcagggcct cctcaacttt cgatcgggtg agtgccgaca cgtcggcgtg accgttcaaa
     1321 actttcgaca cggtcgggac ggagacatcg gcgtgcctgg cgatctcgct cagcgtcaca
     1381 cgaggcttct gctcggcggg ggcgtcggtc tttgcgctca cctatgcagc atagaacgct
     1441 cttgacagcg gcatggaccg tgcctagtgt cgagcggtaa ggaaatattt cgagggcact
     1501 ttcaaaagtt tttatgaaaa cccgcggtga tcgaaggtga tggcgtcgat ggtcgtttct
     1561 cgcatggggt cgaggcgagg aggcggccgc gtcgtcgcgg cggcgcgctc tttcgtgacg
     1621 cctcggcgag tgtggcagac ccaccgaaaa gttgccgcgg cgggtgtttc cgtaactgaa
     1681 ccggttcagc tatgctgggg cccgcttggt cacgattcga ttccgaccat tgggtgacct
     1741 taaccggtta ggtatagttc atgaccaggc gctgcaagag tgttcgcaag tttcgaaaga
     1801 ggtagcgaag cggccgaacc ggtacgcaca cggcgtcatc ggatgcgggc atctttgccg
     1861 agcacctgtc cattcaacga ggaacaaggg cgccgcgtga gcggcggccg caggaaagga
     1921 cctgaccatg gcatcgcaat ccgcgggcgc ttccttcacc aggcgaggct tcattggcct
     1981 tgcggggggc gtggcagcaa catctcttct cgccgcatgc accacaggcg ggggctccgg
     2041 cagcggcggc gcgacgagcg cagcctcggg cgggggcagc tccaagccga tcaagttctg
     2101 gaacatgccg tggggcggca ccgcgttcaa cccgctcgac aagaagatca cgctcgccta
     2161 caagcccaag aagggcctgc ccgccgcgac ttaccaggag atccagtggg cgaacttcac
     2221 ccagaccttc tcctccgcca tcgcctccaa caccggcccg gcagtcagct ccggcggcgg
     2281 cacacaggcc ttccagttcg ccgagcaggg caagatcgcc tacgccgacc accttctcga
     2341 cacctggaag aagaacggtc tttacgacga cttcctcccg ggcgtgctcg attcgatgaa
     2401 gacctcgaag gggtacgcgg ccgtgcccta caacctcgac gaccgtgtca tgtggtacaa
     2461 caaggcgctt ctgcaaaagg ccggtgtcga gccgcccacc gattgggcct cgtataagag
     2521 cgcctgcgcc gccttgaaga agatcggcgt cttcgggttc ggcgtcggcg ccggcactgg
     2581 caacctcatc ggcagccaca tccttactgc gttcatgatc aacaacggcg gaggcctctt
     2641 caacgcgaag caggagcccg cggtcgtcac cgacaggaac gtcgaggcga tcgaatacgt
     2701 tctcgaactc atcaagagcg gctactccga cccgcgcgcg gcgacctaca cgaacgacaa
     2761 cgtctatgcc cagtggaagg cgaacaagtt cggcatgggc tgggacacgg ccggcgcctc
     2821 cgtctcggtc ggcggcacgg tggccgcgca gatggtcgtc ggcgacccgc tgaccggccc
     2881 gcacggcacc aagggcggcc tctacttccc gaacaacatc atgatgtaca cgaacacccc
     2941 cagccaggag ggctcggagg cgttcatgac gtattactac cagcacatga aggagctgtg
     3001 gactaagaag accggcatcg gcctgccgcc gctgaagtcc atcgccgaca cctcggccgt
     3061 gagttccgac ccgaacacgt cgaagatcat caacgtctgg cagccgatct tgaagacctg
     3121 ggcagcaccc ggcggcgccg cgctcttcta caatgtcgcg aacacggtcg acggcaccca
     3181 gccgatgatc acgttcgccc agtcgatctt cggtggtaag acggatgcca aaaccgcgct
     3241 gaccaccctg tcgaactcca tcgaggggct gatgaagtaa caaccacgcg agtgtcgggc
     3301 gggcgagcag gtcttcgtcc gcccgacatc tgcggggcgg caaggcgatg gtgacaaggt
     3361 cgaaggagag ctgatgtcgg tcggatcgat gcgggaatcg ctgaagatgg cccgccgggg
     3421 agtcggtgcg gccggcaacg ggcgggtgcc gaaggcgggc cgcgccggaa acggccgcgg
     3481 caagagcaag atggcacgtt cgcgtgcctc cactttgctg atgttcgcga tcccctcgat
     3541 catccttttg gttctgctga acctgtaccc cgtcgtctac gccgccgagc agtccctgcg
     3601 caacggcgac ctgctctccg ccggaaaatt cgtcgggctg cacaactacg cctcgatgct
     3661 cgactcgccg gtgttctggc atgccgcctg gttcacgctg atcttcaccg tcaccggcgt
     3721 gttcggcagc tgggcgatcg ggttggcgtt ggccctcgtc ttgcgcaccc acatacctgc
     3781 cggcggggtt ttcaaggttc tgcttctttt accctgggtc gtgccgatcg tcgtctcggc
     3841 cacctcgtgg aactggctgg tcgccactcc ccagtcgccg ctgccgattc tcgcgcatgc
     3901 gctcggcctc gggaacatcc tgttcctcgc caaccccgtg atggctcagg tcacggtctg
     3961 tgtcttcaag gtatggatca gcttcccgtt catgatgatg atgatggcct ccgcgttgga
     4021 gtcggtcgac gtcaacgtgt acgaggcgtc gaaggtcgac ggggcgacga gctggcagac
     4081 gttccggcag atcaccctgc cgctcgtctc tcgcacgacc tacatcagct ggatcctgat
     4141 gacgatcttc tgcgtcaacg actttccgac gatcttcctg ctcaccgggg gcgggccggc
     4201 cggcgccacc cagtcgctcg tcgtcttggc ctactcgacc gtgttccaga acttccagac
     4261 cggccccggt gtcgcgatcg cgttcatgat gacccttgtg ctcgtcgtca tctccgtcgt
     4321 gctctaccgt cagattcgaa aggcgaatgt cgcatgagcg tctcagcctc ccccgtcgcc
     4381 ctcgccccgg ccgcgcccca caaggcggac gggcatcatc acgccgagct cggcatgcgc
     4441 gggcgctggt ggcgcttcac acttctcgtc gtcataaccc tgatcgtgct cgtgccggtc
     4501 ggcgtggtcg tgcttctctc cgttcagccg gcgttgggca gcaccaagac cggctggaca
     4561 ctgcagaact tcgccaccgt gttcagccag acctcggtgg gaatctggct gcgcaacagc
     4621 accatcgtca cgctggtgac cgtggtcgtc gccgtggtcg tcgcggcgcc ggccggctac
     4681 gtgctctcgc gcggccgcag caagctggtc tctgggtact ccctcacctt gttcgtggtg
     4741 cagtcgctgc ccgtggtcac ggcgatcatt ccgctcttca tcctgttcgc gaagttcggc
     4801 ctggtcgaca acctggccgg catcacgatc atttacgtcg gctcgaccat ctccgtggcg
     4861 gtgtggatga tggccgccta ctacgactcg atcccgatcg acctggagga ggcctcgtgg
     4921 atcgacggct gctccgtctt gggaagcttc ggccggatcg tcttgcgcaa ctcgctgccc
     4981 ggcatcctgt cgaccgcgat cttctccttc ctcttggcgt ggaacgatta cctcgtcgcg
     5041 ctcgtgttcc tccgcagcga ccacaacttc acgctgcaga tcggcatgga gaccttcttc
     5101 cagcagaacc agaccaactg ggggcttgtc atggcggtcg ccgtcgtcat gatgctgccg
     5161 ccggtcatca tcttcgccgt cttgaaccgt tacttctcgg tcggcggcat cggcggctcc
     5221 ctcgcgggcc
//
LOCUS       DASTNL010000109         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_639271, whole genome shotgun sequence.
ACCESSION   DASTNL010000109 DASTNL010000000
VERSION     DASTNL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639271"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFQ96_03410"
     CDS             <1..666
                     /locus_tag="VFQ96_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEU0256868.1"
                     /translation="EDRFAGPRPRWELAGATVTDDVRPYERVKLRVLNAAHSMLAYLG
                     ALAGHETIAQAVADPRLHSIVADTLERDVLPTLHAPEGMNLEQYRDQVLERFANPSLP
                     HTTLQVAMDGSQKLPIRMLETAAECLRAGRVPHGLATGVAAWIAFIAEAMTGRFMLDD
                     PAAEALRSAVGSPEDARERPRQVVERVSLIPGIFADEVREPRFLTAVADALRPLIPAT
                     GAA"
     gene            1008..2186
                     /locus_tag="VFQ96_03415"
     CDS             1008..2186
                     /locus_tag="VFQ96_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0256869.1"
                     /translation="MGYAACWLTGDVWNLFDIDLTAVTRTWAALAFAGAAVAVTNFCP
                     IRRRRASWLRMSVAGLAIPLFALVAAAGVNVNFGAYPTVADALGANPYRTITVAKIHH
                     SVAPGGRPSAMRSPASWTDWRPSGPLPKSGKVGTVVIPARVPNFTPRPASIYLPPAAE
                     AANAPALPVIVALSGQPGQPADMFVSGRMPAILNRYAAAHHGLAPIVVVPDQLGAPRR
                     NPMCVNSPLGDVATYLTKDVPRWIRAHLHVAPSPGHWAVAGFSEGGTCSIQFAAGYPG
                     LFHTFLDISGEEAPTMGPRTIAAAFHGSPAAYDAAKPLNLLAAHAPYRHTLGIFGVGA
                     ADARYLPQTKTVEAAARRAGMTTSLIVAPGTAHDWRTVRFVMTRALPRIANRLFDGLS
                     "
     gene            2183..>2793
                     /locus_tag="VFQ96_03420"
     CDS             2183..>2793
                     /locus_tag="VFQ96_03420"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0256870.1"
                     /translation="MSSRQSGTRELGDVLEATATPPAGGSLPRLAVRLAGRTPLTATI
                     GLVVAVATIGTWFLPGDGRLDAHVLFAAGFPAVVQEGRWWTPLTSVLVGDDVGDLLLA
                     LAAIGVLCGFAERRMGTLRTLVAYLACSFASIYAGVGLEALLSLGNPFWAAHVSVFGS
                     LDPTRPVVGVIMASSAFCGTLWRRRIRVIVFCVAVVLVLYSGHP"
BASE COUNT          373 a          986 c          958 g          476 t
ORIGIN      
        1 gaagaccgct tcgccggccc gcggccgcgc tgggagctcg ccggcgcgac cgtcaccgac
       61 gacgtgcgtc cttacgagcg ggtcaagctg cgcgtgttga acgcggccca ttcgatgctc
      121 gcctacctcg gtgcgttggc cgggcacgag acgatcgcgc aggccgtcgc cgacccgagg
      181 ctgcacagca tcgtcgccga caccctcgaa cgcgacgtgc tgccgaccct ccatgcccca
      241 gaaggcatga acttggaaca atatcgcgac caggtgttgg agcgcttcgc gaacccaagc
      301 ttgccgcata cgacgctcca ggtggcgatg gatggctcgc agaagctgcc gatccggatg
      361 ctggagacgg cggccgaatg cctgcgcgcg ggccgcgtgc cgcacggcct cgccaccggg
      421 gtcgcggcct ggatcgcttt catcgccgag gccatgaccg ggcggttcat gctcgacgac
      481 ccggccgccg aggcccttcg atcggccgtc ggctcgccgg aagacgcccg cgagaggccg
      541 cgccaggtgg tcgagcgggt ttccctgata cccggcatct tcgccgatga ggtgcgggaa
      601 ccgcggttcc tcacggcggt tgccgacgcc ctgcgcccgc ttatcccggc caccggcgcc
      661 gcctgagcca gacgggccgc ccgggtatca gacaggcttc tggtgaggag gcgtcccggt
      721 ttacggtcag ggatcttcat gctgccttgc aggatcgcac ccggaaaatg tggaggccgg
      781 gttaggggcg acagagacgt gaggtggccg ccgtgctgca ctgggtgctc acgagaaata
      841 tcgagggcgc gggtttcctc gtgccgctct atgcggcagg cgcggtcgtt tgtctgtatc
      901 tgctggccta accgtgggtg cgcaggcacg tcggccaccg gtcgagacga tcgctgctgc
      961 ctgcgggtgg agtccgcctc ttcgtagccg ggctcacggg cctggcggtg ggttacgccg
     1021 cctgctggct gacgggcgac gtctggaacc tgttcgacat cgacttgacg gcagtgaccc
     1081 gcacgtgggc ggcgctcgcc ttcgctgggg ccgccgtggc ggtgacgaac ttttgtccga
     1141 tccgtcggcg gcgggccagc tggttgcgaa tgagcgtcgc cggcctcgcc atcccgctct
     1201 tcgccctcgt ggcggcggcc ggtgtcaacg tgaatttcgg cgcctacccg accgtcgccg
     1261 atgcgctggg ggccaaccct taccgcacca tcacggtggc gaagatccat cacagcgtcg
     1321 cacccggcgg ccgcccctcg gctatgcggt cgccagccag ctggaccgat tggcgcccct
     1381 ccggccccct gccgaaatcc ggcaaggtgg gcaccgtcgt catcccagcg agggtgccca
     1441 atttcacgcc gcggccagcc tcgatctatc tgccgccggc ggcggaggcg gcgaacgcgc
     1501 ccgcgctgcc ggtgatcgtc gcgctctcgg gccagccggg ccagcccgcc gacatgttcg
     1561 tctccggacg gatgccggcg attttgaacc ggtacgctgc agcccatcat ggcctggccc
     1621 cgatcgtcgt ggtgcccgac cagctgggtg cgccccgccg gaacccgatg tgcgtcaact
     1681 cgccgctcgg agacgtggca acctatctca cgaaggacgt gccgcggtgg attcgcgctc
     1741 atctgcacgt ggcgccgagc cccgggcatt gggcggtcgc cggattctcc gaaggcggca
     1801 catgctcgat ccagtttgcg gcaggctacc ccggcctctt tcacacgttc ctcgacattt
     1861 caggtgagga ggcgccgacg atggggccgc gtacgatcgc ggccgccttc cacggctcgc
     1921 ccgccgcgta tgacgcggcg aaacccctca acttgctggc cgcccacgcg ccctatcgcc
     1981 acaccttggg catcttcggg gtcggtgcag cggatgcgcg atacctcccg cagacgaaga
     2041 cggttgaggc cgccgcacgg cgggcgggaa tgacgacgag cctcatcgtg gcacccggca
     2101 ccgcgcacga ctggcgtacc gtgcgcttcg tcatgaccag ggcgctgccc cggatcgcga
     2161 atcggctctt cgacggcctg tcatgagcag ccgtcagagc ggcacgcggg agctcggtga
     2221 cgtgttggag gcgacagcga caccccccgc cggaggatcc ttgccgcggc ttgccgtgcg
     2281 cctggccggc cgcacgccgc tcaccgcgac gatcggcctg gtcgtcgccg tcgcgacgat
     2341 cggcacatgg tttctgcccg gcgacgggcg cctggacgca cacgtgcttt tcgccgccgg
     2401 gtttcccgcg gtcgtgcaag aaggtcgctg gtggaccccg ctgacctcgg tgctggtcgg
     2461 tgacgacgtg ggcgacctgc tcctggccct ggccgcgatc ggggtcttgt gcgggttcgc
     2521 cgaacgccgg atgggcacgc tgcgcaccct cgtcgcctac ctggcctgct ccttcgccag
     2581 tatctacgcc ggcgtcggcc tggaggcgct cctgagcctc ggcaacccct tctgggcggc
     2641 gcatgtcagc gtcttcggct cgctcgaccc gaccaggccg gtggttggcg tgattatggc
     2701 gtcgagtgcg ttctgcggca cgctgtggcg acgacgcatc cgggttatcg tgttttgcgt
     2761 cgccgtcgtc ctcgtgctct actccggcca ccc
//
LOCUS       DASTNL010000110         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_454578, whole genome shotgun sequence.
ACCESSION   DASTNL010000110 DASTNL010000000
VERSION     DASTNL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454578"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /locus_tag="VFQ96_03425"
     CDS             <1..2028
                     /locus_tag="VFQ96_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0256871.1"
                     /translation="GGGRVETPLTATVRVPRSPGLRGWLRLDRPVSVATTPPRMWLLC
                     AAGLVLGLAAGGAAVALFIMIGFLTHLTLLHDIGTDLPTLRGWKPTLWIIPTAVAGAF
                     VVSLFAKWSPVIRGHGIPESLEAILTRHSRIRPRAAVAKPISAVITIGTGGPFGAEGP
                     IIVTGGSFGSLLGQVLSVSPAERKILLATGAAAGMAATFNTPVAAVILAVELVLFERS
                     LRTIVPLALACGVAASVHILAFGTNPLFFIPGILHVSFLHLPWFLLVGLASGIMAVIL
                     NKGLFLMEAGFRHLPVNIFWWPMIGALIYSVIGYFEPRTLSMGNSAIIDTIEGHFVLG
                     AVAVLFVAKLFSWWVALGSQTSGGTLAPMFLVGALMGSLIGHGVNAVFPAANVSTGAF
                     ALAAMAAVFGASTKAVLASVIFAAEVTGQYQMVIPLLIATAVADLVAQVGLGDRIMTE
                     KLSHRGFRVDFDMVTDVLRQRLVRHAMRGPVTIPATATVANAHQLLVENGLNALAVAD
                     SRQKYVGLATDAIVAAAPPDALMCDIPLADQKPTVRSDYLDIILTRMTNENLDALPVV
                     QDGTVVGLITQGHILAYRHHARALAETRQPGWWATRHEPRKTAMAMAAHRATERATQR
                     ATRAGSARPPAARGTAGAKTQVRPAPAQDVEERDAQTATLAHPHHLDGGGY"
     gene            2114..3067
                     /locus_tag="VFQ96_03430"
     CDS             2114..3067
                     /locus_tag="VFQ96_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU0256872.1"
                     /translation="MTAIVLDPSPIAAGYRDQVRGDVARLTARYGRPPRIRGLLAQHE
                     GPAATYARYARRGCESVGIELDVHHTDAGEVARAIAAANVDPEVDGIFLYYPLTDPTG
                     DRWLRELVDPRKDVEGLHSFWSRLLYENVRFVDAERTIPAILPCTPLAIVKLLQHAGM
                     RGTRAGAPLEGVTACVINRSNLVGRPLAAMLANDGARVHSLDIDGSVTFEPAIGRRTH
                     DLREPGVDRETALAGADVVISAVPSQEFDLIRGDEIRAGAICVDVAEYTNFDKSVLDR
                     AGVFVPRVGPLTIAMAMRNLTRLAAVKSPLPRVGAPTTRGL"
     gene            complement(3159..4229)
                     /gene="zapE"
                     /locus_tag="VFQ96_03435"
     CDS             complement(3159..4229)
                     /gene="zapE"
                     /locus_tag="VFQ96_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEU0256873.1"
                     /translation="MSSTVPALVTHVTERVPATSAAELVGRLVPPPQFARASFDSYRP
                     DKAYPSQAAAVETLRRFATSASGHPGGFFSRWHKQPETPPGVYLDGGFGVGKTHLLAA
                     LWHSVAGRTYYGTFIEYTALVGALGYRQTVDLLAGADLLCIDEFELDDPGDTMVMTRL
                     IGALVDHGTKIAATSNTPPNALGEGRFAAHDFLREIQSIARSFRTLRIDGLDYRRRDA
                     AQAAQVLDPEDLGRAVDALASRGKTVSFDAFRDVVDHLAEVHPSRYADLVGGLDVVAL
                     SDVTVLHDQTEALRLVAFIDRVYDLQIPVLASGAPLDGVFDAEMLAGGYRKKYLRAAS
                     RIVALTSGELPPGDADAGSAHA"
     gene            4431..5330
                     /locus_tag="VFQ96_03440"
     CDS             4431..5330
                     /locus_tag="VFQ96_03440"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0256874.1"
                     /translation="MSVEYDSSAEFAQFAHPERLVSADWLEKHRGDKGLVIVESDEDV
                     LLYDTGHIPGAVKIDWHTDLNDPVERDYVDGKRFAELLGSKGIGRDTTVVIYGDKNNW
                     WAAYALWVFTLFGHEDVRLLDGGRDKWVADGRELTTEVPTPTPVDYPVVERSDAKIRA
                     FKEDVLGHFGNPLIDVRSPQEYSGERTEIPGYPTENALRAGHIPTAASVPWSKAAAAD
                     ATFKSRPELEKIYLEDAGLKSGDDVITYCRIGERSSHTWFVLTHLLGLEKVRNYDGSW
                     TEWGSAVRVPITLGAERGAAPQR"
     gene            5433..>5876
                     /locus_tag="VFQ96_03445"
     CDS             5433..>5876
                     /locus_tag="VFQ96_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEU0256875.1"
                     /translation="MSDDQLAPGLAQVRDEFLELEQPERLQLLLEYANTLPELPERYA
                     SRPELFERVVECQSPVYLFVEVTDDIVTLFATAPKEAPTTRGFASILVRGLSGLHVAE
                     VLAVPDEFPQMLGLTRAVSPLRLRGMSGMLGRTKHQVKEQVAQAGS"
BASE COUNT          927 a         2060 c         1964 g          925 t
ORIGIN      
        1 ggcggggggc gggtcgagac tcccctgacc gcaaccgtgc gagttccccg gtcaccgggg
       61 ctgcgcgggt ggctgcgatt ggaccgcccg gtctcggtcg cgacgactcc gccacgcatg
      121 tggctccttt gcgcggcggg cttggtctta gggctggccg caggcggcgc cgccgtcgcc
      181 ttgttcatca tgatcgggtt cctcacccat ctgaccctgt tgcacgacat cggcaccgac
      241 ctgcccacat tgcggggctg gaaaccgacg ctgtggatca tcccgacggc cgtggcaggc
      301 gccttcgtcg tgtcgttgtt tgcgaaatgg tcgccggtga tccgcgggca cggtatcccc
      361 gagtcgctcg aggccatcct gacgagacac agccggatcc gcccgcgcgc ggccgtggcc
      421 aagccgatct ccgcggtcat cacgatcggc acgggcgggc cgttcggggc ggaggggccg
      481 atcatcgtga cgggcggctc gttcggctcg ctcttgggcc aagtgctgtc cgtctccccg
      541 gcagagcgga agatcttgct agccacaggt gctgcggcag gcatggcggc cacgttcaac
      601 actccggtgg ccgccgtgat cctcgccgtc gagctcgtgc tgttcgagcg ttcgctgcgc
      661 acgatcgtgc cgttggcgct ggcctgcggg gtggccgcgt cggtgcatat cctcgcgttc
      721 ggaacgaacc cgctcttctt cattcccggc atactccacg tgagcttcct gcacctgccc
      781 tggttccttc tcgtgggtct cgcctccggc atcatggcgg tcatcctgaa caagggcctc
      841 ttcctcatgg aggccgggtt ccgtcatctg ccggtgaaca tcttctggtg gccgatgatc
      901 ggggcgctga tctactcggt catcggatac ttcgagcccc gcacgctgtc gatgggcaat
      961 tcggccatca tcgacacgat cgaaggacac ttcgtgctgg gcgccgtcgc ggtgctgttc
     1021 gtcgcgaaac tgttctcgtg gtgggtcgct ctcggttcac agacctccgg tggcacgctc
     1081 gccccgatgt tcctcgtcgg cgccttgatg ggcagcctca tcgggcacgg cgtcaacgcg
     1141 gtgttccccg cagcgaacgt gtcgaccggg gccttcgccc tggcggcgat ggccgccgtg
     1201 ttcggcgcct cgacgaaggc cgtgctcgcc tcggtcatct tcgcggccga ggtcaccggg
     1261 caataccaga tggtcatacc gctgctcatc gcgacggccg tcgccgacct ggtcgcacag
     1321 gtgggcctgg gagaccggat catgacggag aagctgtcgc accgcgggtt ccgggtcgac
     1381 ttcgacatgg tcaccgacgt tctccggcaa cggctcgtac gccatgcgat gcgtggcccc
     1441 gtcaccatcc cggcgacggc gaccgtcgcc aatgcccacc agctgctcgt cgagaacggc
     1501 ttgaacgcgt tggccgtcgc agactcacga cagaagtacg tggggctggc caccgacgcg
     1561 atcgtggcag cggcaccccc cgacgcgttg atgtgcgaca tccccctcgc cgatcagaag
     1621 ccgaccgtga gaagcgacta tctggacatc atcctcaccc ggatgaccaa cgagaacctc
     1681 gacgccctcc cggtcgtgca agacggcacg gtggtgggcc tcatcaccca agggcacatc
     1741 ctggcctacc ggcaccacgc cagggcgctg gccgagacca ggcagccggg ctggtgggcc
     1801 acgcgtcacg aaccgcgcaa gacggcgatg gcgatggccg cgcaccgtgc caccgaacgc
     1861 gccacccaac gcgccacccg tgccggctcg gcccggccgc ccgcggcgcg cggcaccgcg
     1921 ggcgccaaga ctcaggtgcg ccccgccccg gcgcaagacg tcgaggagcg tgacgcccag
     1981 acggcgacgc tcgcccaccc gcaccactta gacggtggcg gttactgagc cgcgcgcctt
     2041 ataggatcga ggaggccgac ggcgggaccg cggcgcccac caccatgcag gcagcgacac
     2101 gggaggatgc cttgtgaccg ccatcgtgtt ggacccgtca ccgatagccg ccggttaccg
     2161 cgatcaggtg cggggcgacg tcgcgcggct cacggcacga tacggccgcc cgccgcgcat
     2221 ccgcggtctg ctcgcccagc acgaggggcc tgccgccacc tacgcccgct acgccaggcg
     2281 cggctgcgag tcggtcggca tcgagctcga cgtgcatcac acggatgccg gcgaggtggc
     2341 ccgggcgatc gccgccgcga acgtcgaccc cgaggtcgac ggcatcttcc tgtattaccc
     2401 gctcaccgac cccacggggg accggtggct gcgagagctt gtcgatcccc gcaaagacgt
     2461 cgagggcctc cactcgttct ggagcaggct gctctacgag aacgtgcggt tcgtcgacgc
     2521 cgaacgcacg attcccgcga ttctgccctg cacgccgctg gccatcgtga agctgctgca
     2581 gcacgccggc atgcgaggca ctcgggccgg agcgccgctc gagggcgtca cggcctgcgt
     2641 catcaaccgc agcaacctcg tcggccgccc gctggcggca atgctcgcca acgacggggc
     2701 gcgggtccac tcgctcgaca tcgacgggtc ggtcacgttc gagccggcga tcgggcgacg
     2761 cacgcacgac ctccgcgagc ccggggtcga tcgcgagacc gctctcgccg gcgccgatgt
     2821 cgtcatctca gccgtgccgt ctcaggaatt cgacctcatc cggggtgacg agatccgggc
     2881 aggggcgatc tgcgtcgacg tcgccgagta cacgaacttc gacaagtccg tcctcgatcg
     2941 ggcgggcgtc ttcgtccccc gggtggggcc cctgacgatc gccatggcca tgcgtaacct
     3001 cacccgcctg gccgccgtga agtcacccct gccgcgcgtc ggtgcgccga cgacgcgcgg
     3061 cctctgaccg gcaagacgtg cccgcacatg cggcctcgac cggccgtgca tgccgcctca
     3121 ccacggcctc cggcagtgac ggtgacagcc ccgaagggtc aggcgtgcgc cgagcccgca
     3181 tcggcgtcac cgggcggcag ttcgccgctg gtcaacgcca cgatgcgcga ggcggcccgc
     3241 aggtatttct tgcggtaccc gccggccagc atctccgcat cgaagacgcc gtcgagcggc
     3301 gcgcccgagg cgagcaccgg tatctgcagg tcgtacaccc gatcgatgaa cgcgacgagg
     3361 cgcaaggcct cggtctggtc gtgcagaacc gtcacgtcgg agagcgcgac gacgtcgagc
     3421 ccgccgacga ggtcggcata gcgggaggga tgcacctcgg cgaggtggtc gaccacgtcg
     3481 cggaacgcgt cgaagctcac cgtcttgccg cgggaggcca acgcgtcgac ggcgcgcccc
     3541 aggtcctccg gatccagcac ctgcgccgcc tgcgcggcgt cgcggcgccg gtagtcgaga
     3601 ccgtcgatgc gcagagtgcg aaagcttcgc gcgatcgact ggatctcgcg cagaaagtcg
     3661 tgtgccgcga accgcccctc ccccaaggcg ttcggagggg tgttcgacgt ggcggcgatc
     3721 ttcgtgccgt ggtcgacgag ggcgccgatg agccgtgtca tgaccatggt gtcgcccggg
     3781 tcgtcaagct cgaattcgtc gatgcacaag aggtcggcgc cggccaagag gtcgaccgtc
     3841 tggcgatagc cgagcgcccc gacgagggcc gtgtactcga tgaaggttcc gtaatacgtg
     3901 cggcccgcga cgctgtgcca cagcgcggcc agaagatggg tcttgcccac gccgaacccg
     3961 ccgtcgaggt acacgcccgg cggcgtctcc ggctgcttgt gccaccggct gaagaagcca
     4021 cccgggtgcc cggacgcgct cgtcgcgaac cgtcgtagcg tctcgaccgc cgccgcctgg
     4081 ctcgggtagg ccttgtcagg ccggtagctg tcgaaagacg cacgcgcgaa ctgcggcggc
     4141 ggcacgagcc tgccgacgag ctccgcggcc gaggttgcag gaacacgctc ggtcacatgc
     4201 gtcaccaacg ccggcacggt cgaactcatg gcgaatcgtt caccttcaca ctctcgggtt
     4261 cgtcccgcgt cgcggtacgg cgcgggcacg gccggattat gtcgtagtct tacgcatccg
     4321 gctctttgcc tgccgaacgg acgtagattc gtagcggggc gggcagcaca agcctaatcc
     4381 gaccccaaca catcgttcgc accgtcacgg tgccggttca ggaggaacac atgagcgtcg
     4441 agtacgactc gtctgcagag ttcgcccagt tcgcgcatcc ggagcgtctc gtcagtgccg
     4501 actggctcga gaagcaccgc ggagacaagg ggctcgtcat cgtcgaatcg gacgaagacg
     4561 ttctgctcta cgacactggt cacatccccg gggcggtgaa gatcgactgg cataccgacc
     4621 tcaacgaccc ggtcgagcgc gactatgtgg acggcaagcg tttcgccgag ttgctcggct
     4681 cgaagggcat cggccgcgac acgaccgtcg tcatctacgg cgacaagaac aattggtggg
     4741 ccgcatacgc cctttgggtg ttcaccctgt tcggccacga agacgtgcgg ctgctcgacg
     4801 gaggtcgcga caaatgggtc gccgacggcc gtgagttgac gaccgaggtg ccgacgccga
     4861 cgcccgtcga ctaccccgtc gtcgagcgct ccgacgcgaa gatccgtgcc ttcaaagaag
     4921 acgtcttggg gcacttcggc aacccgctca tcgacgtgcg cagcccgcaa gagtacagcg
     4981 gcgaacgcac ggagatcccg ggctacccaa cggaaaacgc actgcgcgcc gggcacatcc
     5041 cgacggcggc ctccgtgccg tggtcgaagg cggcggcggc cgacgccacc ttcaagagcc
     5101 gccccgagct ggagaagatc tacctcgaag acgccggcct gaagtcgggc gacgacgtca
     5161 tcacgtactg ccgtatcggt gagcgttcca gccacacctg gttcgttctc acccacctct
     5221 tgggtctgga gaaggtgcgc aactacgacg gctcctggac ggagtggggc agcgcggttc
     5281 gggtgccgat caccctgggc gccgagcggg gtgccgctcc gcagcgctga cccgaccaag
     5341 gcgccgcgca tgccgcgccg gcgtcgtacg agaggggtcc gggccgccaa gaggcccggg
     5401 ccccttcgtc gtctcgtggg atactgaaga caatgagtga cgaccaattg gcccctggcc
     5461 tcgcgcaggt ccgcgacgag ttcctcgaac tcgaacagcc ggagcgcctg caactgcttc
     5521 tggagtacgc caacacgctg ccggaactgc cggagcgcta cgcgtcacgc cccgaactct
     5581 tcgagcgcgt cgtcgaatgc cagtccccgg tgtatctctt cgtcgaggtc accgacgaca
     5641 tcgtcaccct cttcgccacg gcgccgaagg aggcgccgac gacgcgtggc ttcgcgtcga
     5701 tcctcgtgcg cgggctgagc ggcctgcacg tggcggaggt gctggcggtg cccgacgagt
     5761 ttccccagat gctgggcctc acccgcgcgg tctctccgct gcggctgcgc ggcatgagcg
     5821 gcatgctggg ccgcaccaag caccaggtca aggagcaggt cgcgcaggca ggttct
//
LOCUS       DASTNL010000111        10839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1564772, whole genome shotgun sequence.
ACCESSION   DASTNL010000111 DASTNL010000000
VERSION     DASTNL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564772"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..579)
                     /locus_tag="VFQ96_03450"
     CDS             complement(157..579)
                     /locus_tag="VFQ96_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0256876.1"
                     /translation="MTLVVDASALSEYLIGSRRGLAAARAFAGHERSLHMPHLAVSEV
                     TSVLRSWVAREDVTESRARGALEDLADFPATRYAAEPFLTRIWQLRHNLSSYDAHYVA
                     LAEALAAPLLTADARIAHASGHHADVLLLGETHPTTQP"
     gene            complement(576..809)
                     /locus_tag="VFQ96_03455"
     CDS             complement(576..809)
                     /locus_tag="VFQ96_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256877.1"
                     /translation="MSSLQIRNVPEDVLRTLKARASAAGQSLSEYALGELAATTARPT
                     LDELNERIRLRGHVDPDTSAAETLHAERTEHAA"
     gene            1254..2546
                     /locus_tag="VFQ96_03460"
     CDS             1254..2546
                     /locus_tag="VFQ96_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HEU0256878.1"
                     /translation="MDAPVHADALTHAEDLAGFVAASPSSYHAAAEVARRLEDAGFTR
                     VDEAAPWPAQPGGRFVVVRDGAVIAWTVPTNATPTTAAHVFGAHTDSPAFRLKPEPTT
                     GADGWLQAGVEVYGGALLNSWLDRELRLAGRLVLDDGSVRLAATGPLLRLPQLAIHLD
                     RSQNDGLALDRQRHTQPVWGLGDAESADLLRVVAAHAKIDPARIRGYDLVTADAARGQ
                     VFGNDDVFFASGRLDDLASVHAGLAALLRTAQEENTPHIALVAAFDHEEVGSATRSGA
                     AGPFLADVLDRVWRSLGAGGDERARALASSWCVSSDVGHSVHPNFPERHDPVVRPVLG
                     SGPILKINANQRYATDAVGAAAWEGWCQAAGVRGQQFVSNNAVPCGSTIGPMTATRLG
                     IRTVDVGIPILSMHSARELAGIADLWALSRVAESFLRS"
     gene            complement(2780..3364)
                     /locus_tag="VFQ96_03465"
     CDS             complement(2780..3364)
                     /locus_tag="VFQ96_03465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0256879.1"
                     /translation="MSEHELSLAGPEVRLDPIVPGDAETLWRMTDRELWFGMISPWPA
                     SPAAYAARIAAQRARPGTIAFTVRSARDGEVRGTTSFYELVPEQSRVEVGSTWYGRPF
                     WGGRTNPAAKLLLLTHAFETLGLNRVGLRCDSRNTRSATAIRRLGAKPEGVLRSHRIA
                     ADGSVGDTAYFSIIRAEWPEVRAGLEARLRHPEA"
     gene            3519..4904
                     /locus_tag="VFQ96_03470"
     CDS             3519..4904
                     /locus_tag="VFQ96_03470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU0256880.1"
                     /translation="MAAASRLDSVIALARHRGFVFQAGEIYGGSRSAWDYGPLGVELK
                     ENIKRQWWRTMVTGRADVVGLDSSVILPKEVWVASGHVSVFNDPLVECLSCHKRFRED
                     HLLEAYEAKKGHPAPNGMADVACPNCGTRGQWTPPRDFNMMLKTYLGPVQDESGLHYL
                     RPETAQGIFVNFANVLQASRMKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFF
                     VKPGDDETWFDYWLEERRRWYLDLGIDPANLRFFEHPKEKLSHYSKRTIDIEYRFGFT
                     GGEWGELEGIANRTDYDLSTHAKHSGKDLSYFDQAAGERWTPYVIEPAAGLTRSLMAF
                     LVDAYHEEEAPNAKGGTDKRTVLRLDPRLAPVKAAVLPLSRNERLSPLARELADRLRG
                     LWNVDFDDAGAIGRRYRRHDEIGTPFCVTVDFDSLDDHAVTVRDRDTMAQERVSLDKI
                     DDYLAVRLKGA"
     gene            4910..6139
                     /locus_tag="VFQ96_03475"
     CDS             4910..6139
                     /locus_tag="VFQ96_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HEU0256881.1"
                     /translation="MPDTADAGGQPFSAGRAATPAQVRKWRRYLADERAEAAVYRDLA
                     ARRAGEEREILLALAEAEGRHEDHWLTLLGARRGRPARGDARTRVLGFLARRFGSVFV
                     LALAQRAEARSPYETEADATATMAADERIHEEVVRALAARGRRRLSGSFRAAVFGVND
                     GLVSNLSLVLGIGAAGVATHVVLLTGVAGLLAGALSMAAGEYVSVRSQRELLEASRPS
                     RQAALALPHLDLDANELTLVYRARGMSQADAERHAREVLGALRRPAPRLSPPVADVPH
                     DSAVTGLPEDDRATDLPDDAPATSLVSGGPGTAAGADEHEAVGTGATAALSSFCFFAA
                     GALIPVVPYLFGLQGLAAVALAAGMVGVTLLGTGAAVGLLSGAPPLRRALRQLAIGYG
                     AAAVTYLLGLALGGVVG"
     gene            complement(6146..6883)
                     /locus_tag="VFQ96_03480"
     CDS             complement(6146..6883)
                     /locus_tag="VFQ96_03480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0256882.1"
                     /translation="MSIKASSAHAGAHNTLTARQQLEAAREAEAMLAATQSSPRMRAL
                     PLRAAELRTPRLVLRPRVDPRSLPQGDTTRSATSTGEPTDEQRAEAAQYLQWEAHDEA
                     QALDEYAARRARDRLEVDGDGLTFTVELPNDDGSGRVIGQASIFLASAASAQMEIGWV
                     FHPAMHGRGYAAEAVEALLRLCFETLDAHRVMVKLDPRNEASARLCESLGMRREGRMA
                     ENTFTDDAWRDTSIHAILHREWAAITC"
     gene            complement(7006..8406)
                     /locus_tag="VFQ96_03485"
     CDS             complement(7006..8406)
                     /locus_tag="VFQ96_03485"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU0256883.1"
                     /translation="MLFCYSSSHQTTGFDTLEGLGRGTAEVTRRLAHSEGVAGSVVLS
                     TCNRFEVYLDVPGAAEGAALATDAIGEATGMDETTVRDAGTMLHGPDAVEHVISVASG
                     LESVVVGEGEIAGQVRRALESARRNNTVSRSLERAFEQASRTSRGVKNRTDLSAAGRS
                     VVRLALDLAESRVTDWAHTHVLLVGTGRYAGASLAALRERGVRHVAVFSVSGRAQGFA
                     KNHDIRAVPAGDLVDAVAASDVIVTCTVAPSVVLDADIVTAARTREGALPRRLIIDLG
                     LPRNVDPAVTAVPETELLDLETIGKHAPLPELRAAREARALVDKAVADYRRANAEDTV
                     TPALVALRRHVFDVLDGEIAWARRRGDVSPATETALRHLTGVLLHTPSIRARALAREG
                     RAADFAAGIDALFGVRPPEEPDEAERLCPVTDVAEARRSHAAQAAEAADAAPADDGMA
                     LQAPAGHKTTLGVDAS"
     gene            8619..9722
                     /gene="hemE"
                     /locus_tag="VFQ96_03490"
     CDS             8619..9722
                     /gene="hemE"
                     /locus_tag="VFQ96_03490"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU0256884.1"
                     /translation="MLPAGHPLAAGVTTAAPLVRAYRGDRPGRTPVWFMRQAGRSLPE
                     YRALRRGVAMFDACLDPAMACEITLQPVRRYGVDAAVFFSDIMVPLRLAGVPVEIVPG
                     TGPVVEAPVRTADDVAALVDHRLTAEPSGPLAPIIEAVRLTVAELRGTPLVGFAGAPF
                     TLAAYLVEGGPSRDHLAARTMMHADPASWARLMAWCADVTGMFLQVQLAAGASAGQLF
                     DSWAGSLSRADYVASVAPASARALASARLREPGGTVVPLVHFGVGTGHLLADMLRAGG
                     DVAGVDYRTPLDEAAQALGPEVPLQGNIDPAMLAAPWPVLEAHVRDVLRRGGAARSHV
                     VNLGHGVPPATDPDVLSRIVDLVHETPQPARAS"
     gene            9719..>10839
                     /gene="hemG"
                     /locus_tag="VFQ96_03495"
     CDS             9719..>10839
                     /gene="hemG"
                     /locus_tag="VFQ96_03495"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU0256885.1"
                     /translation="MTGPTAAESQGRAAGPAAQRAPHAAVGNDRLPPTRVLVIGGGAA
                     GLVAAWDCARPGFEVTLVEASERTGGSVAAHEVAGVTLDVGAESFATRGGAVEELLGD
                     LGLRDRIVAPNPAGAWLHAAGRSVPLPRGGMLGIPASPLARDVIAAIGWRGALRAYAD
                     RLVPVMKIGREENLARLVARRMGRAVLDDLVAPVVTGVYSTPVEDLDVEAAVPGLNGA
                     LTRAGSLSGAVAELRLAAARRAKEAGTGSKPGSAVLGLQGGMHTLVAALVEGLRARGG
                     RIVTGTRALAVRPLSVHDEGQTPPAFGPGWRVETAGGESLTADAVVVAAPQAEAVALL
                     AEASEAAVGLAALAWPQAGRVDIVTLVLDQPALDAAPRGT"
BASE COUNT         1424 a         3877 c         3844 g         1694 t
ORIGIN      
        1 ggatgatccc ggcgcctgcg cggcgaactt ggacagattc gtgtgttccc cgcgccagcg
       61 gggatgatcc cgtgaagtac cacgatatcg gcgccggaat gctgtgttcc ccgcgccagc
      121 ggcgtgatcc cgtgggtcga cggcgccggc cggtggtcat ggctgggtcg tggggtgcgt
      181 ctcgccgagg agcagcacgt cggcgtggtg gccggaggcg tgggcaatgc gagcgtcggc
      241 agtcaggagg ggtgccgcca aggcctccgc gagggccacg taatgggcgt cgtaactgct
      301 tagattgtgg cgaagctgcc agatgcgggt caggaacggc tcggcggcgt agcgggtcgc
      361 cggaaagtcg gcgaggtctt cgagcgcgcc gcgggcgcgc gattctgtaa catcttcgcg
      421 cgccacccag gagcgcagca ccgaagtgac ctccgagacg gcgaggtgag gcatgtgcag
      481 agaacgctcg tgcccggcga acgcgcgtgc cgcagccagc ccgcgtcgag agccgatcag
      541 gtattcgctg agtgccgatg cgtcaacgac gagcgtcatg ccgcgtgctc ggtgcgttct
      601 gcatgcagcg tctctgcggc ggaggtatct ggatctacat ggccgcgcag ccggatccgc
      661 tcgttcagct cgtctagggt cggccgcgca gtggtcgctg cgagctcgcc gagcgcgtat
      721 tccgacaggc tctggcctgc ggcgctcgcc cgcgccttca gcgtgcgcaa cacgtcctcg
      781 ggaacattgc ggatctgcag gctggacatg tattaagcat atctgacatg agttatgcat
      841 gtattcacga tgtggcctaa cgctgtacta ccgtctcttc gtaccaccgg gacatctgtg
      901 ggccgtgcgg gagcaggtca tgctgcggtg ctcgctggtc ggcgacggag tcgagcgctt
      961 cggcgtaacg tgcccacagc gctccacctc gaactcgcgc gcacgacacg gtcactgcct
     1021 ctggccgaac tcgaaccgca gaatacgtat gcggcgcttc gtgctctcgc tggcgggcgg
     1081 catccggccg tgcttggcgc ccgccaccca cggctggatc gccgcggcac cgcctcaccg
     1141 gcatcggtgg ttcaggtggc gtgctcgtca aggccggtgc cgacgcccac agggcccagt
     1201 agcatcgaac tatcgcagct acccaccatt cgggaggcac cctgaccccc gccattgacg
     1261 cgccggtcca cgccgacgcg ctgactcacg ctgaagacct ggccgggttc gtcgcggcgt
     1321 cgccgtcgag ctatcacgcc gcggccgagg tggcgcgccg gctcgaggac gccgggttca
     1381 cgcgtgtcga cgaggccgcg ccgtggccgg cccaaccggg cggccgcttc gtcgtggtgc
     1441 gtgacggtgc cgtcatcgcg tggacggtgc ctacgaatgc gacgccgacg acggctgcgc
     1501 acgtgttcgg cgcgcacacc gactcgcccg cattcagact caagcccgag ccgaccacgg
     1561 gcgcggacgg ctggctacag gccggcgtcg aggtgtacgg cggcgccctg ctcaactcgt
     1621 ggctcgaccg ggagctgcgc ctggccgggc ggctcgtgct cgacgatggg tcggtgcgtc
     1681 tggcggctac ggggccgctc ctgcggctgc cgcagctggc aatccatctc gaccgcagcc
     1741 agaacgacgg cctcgcgctc gaccggcagc gacacactca gccagtgtgg gggctcggtg
     1801 atgccgaatc ggccgacctg ctccgcgttg tcgccgcaca cgcgaagatc gaccccgcgc
     1861 gcatccgcgg ttacgacctg gtcaccgccg acgccgcgcg cggccaggtg ttcggcaacg
     1921 acgacgtgtt cttcgcctcg ggccggctcg acgatctggc cagtgtccac gccgggctcg
     1981 ctgcgctgct gcgcacggca caagaagaaa ataccccgca catcgcgctc gttgccgcgt
     2041 tcgaccacga ggaggtcggg tcggcgaccc ggtcgggtgc ggcggggccg tttttggccg
     2101 acgtgctcga tcgggtgtgg cgctccctcg gcgccggcgg cgacgagcgg gcccgtgcgc
     2161 ttgcatcctc gtggtgtgtg tcgagtgacg tcggccattc cgtgcatccg aactttcccg
     2221 aacggcacga tccggtcgtg cggccggtgc tcggctcggg gccgatcctc aagatcaacg
     2281 ccaatcagcg gtacgcgacc gacgccgtcg gcgccgcggc ttgggagggc tggtgccagg
     2341 ccgccggagt gcgcggccag cagttcgtgt cgaacaacgc cgtgccctgc gggtcgacga
     2401 tcggcccgat gacggcgacc aggctgggca tccgcacggt cgacgtcggc atcccgatcc
     2461 tgtcgatgca ctccgcgcgg gaacttgccg gcatcgccga tctctgggcc ctctctcggg
     2521 tcgccgaatc cttcttgcgt tcgtgactga gacgccgcga cgccctcctc acccttcggg
     2581 gggtgtgagg gtgccgcagt gctgggtgtc agggcgtcaa agtgccgtga tgtcaagacg
     2641 tcgaggtgcc ggggtgccgg ggtgccgggg tgccggggcg ccggggcgtt ggaacgtcaa
     2701 ggcgttggaa cattggaacg tctgccgtgg tgcccggagg caccggatgc cggaggtacc
     2761 cgccgtcacg cagggcgtgt cacgcctcgg ggtggcgcag gcgcgcctcg agcccggcac
     2821 gcacctcggg ccactccgcg cggatgatcg agaagtaggc ggtgtcgccg accgacccgt
     2881 cggcggcgat gcgatgcgag cgcaagacgc cttcgggctt cgcgcccagg cgccggatcg
     2941 ccgtggcact gcgcgtgttc ctgctgtcgc agcgcagccc tacccggttc agtccgagcg
     3001 tctcgaatgc atgggtgagg agcagcagct tcgcggccgg gttcgtgcgg ccgccccaga
     3061 acgggcgccc gtaccacgtg gagcccacct cgacgcgact ctgctcgggc accagctcgt
     3121 aaaagctcgt cgtgccgcgc acctcgccgt ctcgggcgct gcgcaccgtg aaggcgatgg
     3181 tgccgggccg cgcccgttgg gccgcgatgc gtgccgcgta ggccgccggc gaggcgggcc
     3241 acgggctgat catgccgaac cagagctccc ggtccgtcat gcgccagagg gtctcggcgt
     3301 cgcccggcac gatcgggtcc aggcgcacct ccgggccggc gaggctcagc tcgtgttcgc
     3361 tcacgaccct aatgtagtgg tcggctcggg cggggctcat aagatggagg cttgaccgcc
     3421 gtgccaggcc cggcctcggc acggccacct tcaccccacc gtcgggtccg tgtccgcccg
     3481 tcgcagcgtc ccccatccga gcatccattg gagccaccat ggccgcagcc tcccgcctcg
     3541 attccgtcat cgccctcgcc cgccaccgcg ggttcgtctt tcaggcgggc gagatctacg
     3601 gcggctcccg ctcggcgtgg gactacgggc cgctcggcgt cgagctgaaa gagaacatca
     3661 agcggcagtg gtggcgcact atggtcaccg gccgggcgga tgtcgtgggt ctcgactcca
     3721 gcgtcatcct gcccaaagag gtctgggtcg cctccggaca cgtcagcgtg ttcaacgacc
     3781 cgctcgtcga gtgcttgagc tgccacaagc gcttccgcga agaccatctg ctggaggcgt
     3841 acgaggcaaa gaaaggtcac ccggcaccga acggcatggc cgacgtcgcc tgccccaact
     3901 gcggcacccg cggccagtgg accccgccgc gcgacttcaa catgatgctg aagacctacc
     3961 ttggcccggt gcaagacgaa tccgggctgc actacctgcg ccccgagacg gcgcagggca
     4021 tcttcgtcaa cttcgcgaat gtgctgcagg cctcccgcat gaagccgccg ttcggcatcg
     4081 ggcagatcgg caaatcgttc cgcaacgaga tcacacccgg caacttcatt ttccgcaccc
     4141 gcgagttcga gcagatggag atggagttct tcgtcaagcc cggcgacgac gagacctggt
     4201 tcgactactg gctcgaagag cgtcgtcggt ggtatctcga cctcggcatc gacccggcga
     4261 acctgcgctt cttcgagcac cccaaagaga agctctccca ctattccaag cgcaccatcg
     4321 acatcgagta ccggttcggc ttcaccggcg gggaatgggg ggagctggag ggcatcgcca
     4381 accgcaccga ctacgacctc tccacccacg cgaagcactc cggcaaagac ctctcctact
     4441 tcgaccaggc cgccggcgag cgctggaccc cgtacgtcat cgagccggcc gccggcctca
     4501 cccgctccct catggccttc ctcgtcgatg cctatcacga ggaggaggca cccaacgcga
     4561 agggcggcac cgacaaacgc accgtcttgc gtctcgaccc gcgactcgcc cccgtgaagg
     4621 cagccgtctt gcccctcagc cgcaatgagc ggctctcccc gctcgcccgg gaactcgccg
     4681 accggctgcg tgggctctgg aacgtcgact tcgacgacgc cggcgcgatc ggccgccgct
     4741 accgccggca tgatgagatc ggcacgccgt tctgcgtcac cgtcgacttc gactctctcg
     4801 acgaccacgc cgtcaccgtg cgggatcgcg acacgatggc gcaagagcgt gtgagcctcg
     4861 acaagatcga cgactacctc gccgtgcgct tgaagggcgc ctgacccgca tgcctgacac
     4921 ggccgacgcc ggcgggcagc ctttctccgc cggccgggcc gcgacgccgg cccaggtgcg
     4981 caagtggcgc cgctacctcg ccgacgaacg cgccgaggcg gccgtctatc gtgacctggc
     5041 ggcccgccgt gcgggggagg agcgggagat cctgctcgcg ttggcggagg ccgaggggcg
     5101 gcacgaggat cattggctca ccctcttggg ggcgcggcgc ggccggcccg cacgcggcga
     5161 cgcacgcacc cgggtgctcg gtttcttggc ccgccggttc ggcagcgtct tcgtgctcgc
     5221 cctcgctcag cgcgccgagg ctcgctcgcc gtacgagacg gaggcggatg cgaccgcgac
     5281 gatggcggcc gacgagcgca tccatgaaga ggtcgtgcgc gcgctggctg cccggggccg
     5341 ccgccgcctc tccgggtcgt tccgcgccgc cgtgttcggc gtgaacgacg ggctcgtctc
     5401 gaacctctcc ctcgttctcg gcatcggcgc agccggcgtc gcgacgcacg tcgtcctcct
     5461 caccggcgtc gcgggcctcc tcgccggggc cctctccatg gcggcaggcg aatacgtctc
     5521 ggtgcgctcg cagcgggagc ttctcgaagc ctcacggccg agtaggcagg ctgcgctcgc
     5581 actgccgcac ctcgacctcg atgcgaacga gctcaccctc gtctaccgcg cccgcggcat
     5641 gagccaggcg gatgcggaac gccacgctcg cgaggttctg ggcgccttgc gccggccggc
     5701 gcctcgcctc tcgccgccgg tcgctgatgt tcctcacgac tccgcggtca cgggtcttcc
     5761 tgaggatgac cgtgccacag accttcccga cgatgccccg gctacgtcgc tcgtctccgg
     5821 tgggccgggc acagccgccg gtgccgacga gcacgaggcg gtcggcaccg gggctacggc
     5881 cgcactgtcg agtttctgct tcttcgcggc cggcgccttg atcccggtcg tgccctatct
     5941 cttcgggctg caggggctcg ccgccgtcgc gctggccgcc ggcatggtgg gcgtgacctt
     6001 gctcggcacc ggcgccgcgg tcgggcttct ctccggcgcc ccgccgttgc ggagggcgct
     6061 gcgccagctc gccatcggct atggcgccgc cgccgttacc taccttctcg gcctggccct
     6121 cggcggggtc gtaggctgag gcgcctcagc aggtgatggc ggcccattcg cgatgcagga
     6181 tcgcgtggat cgaggtgtcg cgccacgcgt catcggtgaa cgtgttctcg gccatgcggc
     6241 cttcgcggcg catgccgagc gactcgcaga gccgggccga ggcttcgttg cgcgggtcga
     6301 gcttcaccat gacgcggtgc gcgtcgagcg tttcgaaaca gagccgcagg agggcctcca
     6361 ccgcctcggc ggcgtagccg cggccgtgca tggccgggtg aaacacccac ccgatctcca
     6421 tctgcgcact ggccgcactc gccaagaaga tgctcgcctg gccgatcacg cgcccgctgc
     6481 cgtcgtcgtt cggcaactcc accgtgaagg tgaggccgtc gccgtcgacc tcgagtcggt
     6541 cgcgtgccct gcgggcggcg tactcgtcga gcgcctgagc ctcgtcgtgc gcctcccact
     6601 gcaggtattg cgccgcttct gcgcgttgtt cgtcggtggg ctcacccgtt gacgtcgcgg
     6661 aacgggtcgt gtcgccctgg ggcagcgaac gcgggtcgac gcggggtcgc aagacgagtc
     6721 gcggagtgcg cagctcggcc gcacgcaagg gcagcgcccg catgcgcggc gaactctgcg
     6781 tcgcggcaag catggcctcg gcctcgcggg ccgcctccaa ctgctgccgg gcggtcaatg
     6841 tgttgtgtgc gcccgcatgc gcggacgatg ccttgatgct cacgtgttca ctcccttgtg
     6901 ttcacggttt tggtgcctca cactcttgca cacctggccc cgggcctgaa atcggcgggt
     6961 cgtggcgggc gtcgcctgca gaggaggtgc ggcgaggcgc ccgcgctagg aggcgtcgac
     7021 gccgagcgtc gtcttgtgcc cggcgggcgc ctgcaaagcc atcccgtcgt cggccggcgc
     7081 cgcatctgcc gcctcggcgg cctgcgcggc gtggctcctg cgcgcttcgg ccacatcggt
     7141 caccgggcag agccgttccg cctcgtcggg ctcttctggc gggcgaacgc cgaagagggc
     7201 gtcgatgccg gcggcgaagt cggccgcccg cccctctcgc gcaagcgcac gggcgcggat
     7261 cgacggggtg tgcagcagca cgccggtcag atgccgcagc gccgtctctg tcgcagggct
     7321 cacgtcgcca cgcctgcggg cccacgcgat ctccccatcg agcacatcga agacgtgccg
     7381 gcgcagggcg acgagtgccg gcgtcaccgt gtcttctgcg ttcgcccggc gatagtcggc
     7441 gacggcctta tcgacgagag cacgcgcctc tcgcgccgcc cgcagctccg gcagcggcgc
     7501 gtgttttccg atcgtctcca ggtcgagaag ctcggtctcc ggcacggccg tcaccgcggg
     7561 atcgacgttg cggggcaagc cgaggtcgat gatgagccgc cgtggcagcg ccccctcacg
     7621 cgtgcgcgcg gccgtcacga tgtcggcgtc gagcacgacg ctcggcgcga ctgtgcacgt
     7681 gacgatgaca tcgcttgccg ccaccgcatc gacgaggtcg ccggcaggca ctgcgcgaat
     7741 gtcgtggttc ttcgcaaacc cctgcgcacg cccgctcacc gagaagacag cgacgtgtcg
     7801 cacgccgcgc tcgcgcagcg ccgcgagact cgccccggcg taccggccgg tgccgacgag
     7861 gagcacgtgg gtgtgcgccc agtcggtcac gcgactctcg gcgaggtcga gggcgagacg
     7921 cacgacggaa cggccggctg ccgaaaggtc ggtgcggttc ttcacgccgc gcgacgtgcg
     7981 actggcctgc tcgaacgcgc gctccagact gcggctcacc gtgttgttac ggcgagccga
     8041 ttccaacgcc cggcgtactt ggcccgcgat ctcgccttcg ccgacgacga ccgattcgag
     8101 gccgctggcc accgagatga catgctccac cgcgtcgggc ccgtgcagca tggtgccggc
     8161 gtcgcgcacg gtcgtctcgt ccatgccggt cgcctcgccg atcgcgtcgg tcgccagtgc
     8221 ggcgccctcc gcggcacccg gcacgtcgag gtacacctcg aagcggttgc aggtggagag
     8281 cacgacgctg cccgccacgc cttcgctgtg ggccagccgg cgcgtgacct ctgcggtgcc
     8341 cctccccaag ccttcaaggg tgtcaaaacc cgtcgtctga tggctggagg agtagcagaa
     8401 cagcactcac tgagcctacg cgcgagcata gagtggcgcc gagtgcgccg tttctatgtg
     8461 ccggtgcggt gttcggcaag aaagtgccgc cggcggcgcc tttcggagct cgtgtcgatc
     8521 ccatctgcga gaatcgtggc gtgtatgccc ctctcgaccc acctggtgcc acggcgcggg
     8581 gcgggtcgcc ctggccctcc gcggccggga ccgacccggt gctgcctgcc gggcacccac
     8641 tcgctgcggg cgtgacgacg gccgcgcccc tggtgcgggc ctatcggggc gaccggcctg
     8701 ggcggacccc tgtgtggttc atgcggcagg cgggcagatc gctgcccgag taccgggccc
     8761 tgcgccgggg cgtcgccatg ttcgacgcgt gcctcgaccc ggcgatggcc tgcgagatca
     8821 cgctgcagcc ggtgcgccgc tatggagtgg atgcggcggt cttcttcagc gacatcatgg
     8881 tgccgctgcg cctggccggc gtgccggtcg agatcgtgcc cggcaccggg ccggtcgtcg
     8941 aggcgccggt gcgcaccgcc gacgacgtgg cggcgctcgt cgatcaccgt ttgacggccg
     9001 agccttccgg gcccctggcg ccgatcatcg aggcggtgcg cctcaccgtc gcggagcttc
     9061 gtggcacgcc gcttgtcggc ttcgccggcg ccccgttcac gctggccgcc tacctcgtcg
     9121 aaggcgggcc ctcccgcgac cacctcgccg cccgcaccat gatgcacgcc gaccccgcgt
     9181 cgtgggcacg gctcatggcc tggtgcgccg acgtcacggg catgttcctg caggttcagc
     9241 tggccgcggg agcgagcgcg gggcagttgt tcgactcgtg ggccggctcc ctctcccgcg
     9301 ccgattacgt cgcctccgtc gcacccgcct ccgcccgggc gttggcgtcg gcgcggctgc
     9361 gggagccggg tggcaccgtg gtgccgctcg ttcacttcgg cgtcggcacc ggccacctgc
     9421 tggccgacat gctgcgcgcg ggcggtgacg tcgccggagt cgactaccgc acgccgctcg
     9481 acgaggcggc acaggcgctc gggcccgagg ttccgctgca gggcaacatt gacccggcca
     9541 tgctggccgc gccgtggccg gtgctcgagg cgcacgtgcg cgacgttctg cgtcgcggcg
     9601 gcgccgcccg gtcacacgtc gtcaaccttg ggcacggcgt gccgcctgcg acggatcccg
     9661 acgtgctgtc ccgcatcgtc gacctggtgc acgagacgcc tcagccggcc agggcgtcat
     9721 gacggggccg actgcggcgg agagccaggg ccgggctgcg gggcccgccg cgcagcgcgc
     9781 gccccatgcg gcggtgggca acgaccggct gccgccgacc cgggtgctgg tcatcggcgg
     9841 cggtgcggcc ggtcttgtgg cggcctggga ctgcgccaga cccggcttcg aggtgaccct
     9901 cgtcgaggcg tcggagcgca ccgggggcag cgtcgccgcc cacgaggtgg cgggcgtcac
     9961 gctcgacgtg ggtgcggaga gcttcgccac ccgcggcggc gcggtcgagg agctcctcgg
    10021 cgatctgggg ctgcgcgacc ggatcgtcgc cccgaacccg gccggcgcgt ggctgcacgc
    10081 cgcggggcgc agtgtgcccc tgccgcgcgg cggcatgctc ggcattccgg cctcaccgct
    10141 ggcgcgagac gtgatcgccg cgatcggctg gcgcggcgcc ctgcgggcct acgccgatcg
    10201 gctggtgccg gtcatgaaga tcgggcgaga agagaatctc gcccggctgg tggcgagacg
    10261 gatgggccgt gcggtgctcg acgacctcgt cgcgccggtc gtcacggggg tctactcgac
    10321 gcccgtcgaa gaccttgacg tggaggccgc ggtgcccggc ctgaacggcg ctctcacccg
    10381 tgccggctcc ctgtcggggg cggtggcgga gctgcggctc gccgcggccc gccgggccaa
    10441 ggaggcgggc accggttcga aacccggttc ggccgtgctc ggtctgcaag gcggcatgca
    10501 tacccttgtg gctgcgctcg tcgagggtct tcgtgcgcgg ggcgggcgca tcgtgaccgg
    10561 cacccgcgcg ctcgccgttc gcccgttgtc ggtgcacgac gaggggcaga cgccgccggc
    10621 gttcgggccc ggatggcggg tcgagacggc cggcggcgaa agcctcacgg ccgacgcggt
    10681 tgttgtcgcg gccccgcaag ccgaggcggt ggcgctcctg gcggaggcct ccgaggcggc
    10741 ggtcggcctt gccgccctcg cgtggccgca ggccggccgc gtcgacatcg tgaccctggt
    10801 gctcgaccag cccgccctcg acgccgcgcc gcgcggcac
//
LOCUS       DASTNL010000112        11020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_268811, whole genome shotgun sequence.
ACCESSION   DASTNL010000112 DASTNL010000000
VERSION     DASTNL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268811"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFQ96_03500"
     CDS             <1..498
                     /locus_tag="VFQ96_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0256886.1"
                     /translation="SRQHLEILWEGDSVTARDLGSTNGSRLDGHTFTESALRPDAVLT
                     IGHTRIVYRLVPRRGREEDARDGRAADPSALGDREPWERDDVEGPDSGDPFRANPYAE
                     DGSEHEGLENPYRDDSRREGSAPGGREPWETEPPEPEGAGRDEGSGEEGSGREPWESE
                     PGERQ"
     gene            498..1091
                     /locus_tag="VFQ96_03505"
     CDS             498..1091
                     /locus_tag="VFQ96_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0256887.1"
                     /translation="MSALTLLVLRIGFLLLLWLFVFAVVYALRSDLFGRRARTLDPAS
                     APRPSGAAPSRPSGGPTTMIAMTPTPAGAAQGVVMKKHQPLATPGASPGTAPAATIGV
                     ARNLVITSGPKKGTTLELGREPITIGRARESVLVLRDDYTSTHHARIMVWNDTWMLQD
                     LGSTNGTFLAGTRIGTPTIVPLDTPIKVGMTTFELRR"
     gene            1101..2351
                     /locus_tag="VFQ96_03510"
     CDS             1101..2351
                     /locus_tag="VFQ96_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU0256888.1"
                     /translation="MPEINHAVAVSHVGKVRANNQDSGYAGRELFLVADGMGGHAGGD
                     VASALALRRIKEADRQYADAAEAEDALHAALLAANTSLTDKVMERPELTGMGTTVSAI
                     CRVGDHMAVAHIGDSRIYLFRDGVLTQVSTDHTFVQRLVDSGRITREEARVHPRRSVL
                     MRVLGDVDASPEIDTFSVATRPGDRWLLCSDGLTGVLHDAAIRRELATGDSPRQTASR
                     LLRHTLDGGAPDNVTIVILDIADDLPGTRSSEPVTVGSAAGPLGAEPAAPAGRTIRLP
                     ALRLRPASPLADPSHFESPSQDYLNELIEEDARRARRRKTFWLVAAIVVALALVAAGF
                     TAYRWTQSMYYVGVHDGQVVIYRGVQQSVGPLSLSHVYERSGIPVSGLDSFDRQQVTE
                     TVNTDSLPDARRIVEHLRDDTDSR"
     gene            2348..3727
                     /locus_tag="VFQ96_03515"
     CDS             2348..3727
                     /locus_tag="VFQ96_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0256889.1"
                     /translation="MTAEAWAPTAPAQTRHRWRLPTRLRNLELLLLVFACAINGAAIL
                     LVQLGALGHVDFALLYYCAALAALVFLLHVCLRVSAPHADPLILPIVTVLNGLGIAEI
                     YRIDLELGQSGWQSAGVRQIAWTAIAVACAIAVILVVRHHRMLQGYTYLFGIGAIVLL
                     LLPMVPGLGRTVFGARIWIAVGPFSFQPGEVAKLFLAVFFAGYLVQHRDALSLVGRTV
                     LGVRLPRLRDMGPILVLWALSMAVIVFQRDLGTALLYFGLFLVMLYVATGRVSWVAIG
                     VVLFAGGALVASRTLPYVTARFQNWLDPFNPHVYDATRGSFQLVQGLFGMAYGGILGT
                     GLGQGRPATVPLAQSDYIIASLGEELGLAGLFAILALYLLFVSRGFRIGFSGRDDFGK
                     LLAVGLAFTVALQCFIVIGGITRVIPLTGLATPFLAAGGSSLTANWIIAALLLRLSDT
                     VHDQPRVVV"
     gene            3729..5183
                     /locus_tag="VFQ96_03520"
     CDS             3729..5183
                     /locus_tag="VFQ96_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0256890.1"
                     /translation="MNRELKRVSVVVLAMFVALLVSTSIIGAVRSDSLAADPRNSFTL
                     ADARLAQRGSILVDGRAIAKSVPSKDQYKYQRVYTNGPVYSSVTGFFPVNGAPTGIEG
                     ALNESLSGTSGSQFLTRLTDTLTGKHPQGASVELTIDPSLQKAAYDALGSLKGAVVVL
                     QPKTGRILAMVSRPTFDPNTLAGHNTAQLDATYKALLAAPTRPLVNRAIGGNLNYPGS
                     TFKPIVAAAALDSGTYTADSTLPDVAALRLPGTDSYVHNAERTKCGDGNTVTITLAMI
                     RSCNIPYAELGMKLGADTIRAAAEKFGFGHAFDIPIPVTPSIYPDDYNVPQTGLSSFG
                     QASTRATPLQMAMVQAAIANGGLVMQPNLVEWVKSANLHTLERFTPKVFDRAMSEQTA
                     NTLRQIMVEDVKKGVASNATIDGVDVGGKTGTSQNGPGLPYTLWFTGFAPAQSPKYAI
                     AVVVEDGGGEGQSGVGNTIAAPIAKKVLEAVLKK"
     gene            5180..6985
                     /locus_tag="VFQ96_03525"
     CDS             5180..6985
                     /locus_tag="VFQ96_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0256891.1"
                     /translation="MRPTAGLTFGGRYELQSRIAIGGMGEVWQATDSIIGRTVALKIL
                     KDEYLGDPGFLERFRAEARHAALVNHEGIANVYDYGEEDGSAFLVMELVPGEPLSAVL
                     EREHTLTTDRVLDIVAQTAAALHAAHSAGLVHRDIKPGNLLITPDGRVKITDFGIARV
                     ADQVPLTATGQVMGTVQYLSPEQASGHPASPSTDIYSLGIVAYECLAGRRPFTGESQV
                     AIAMAQINEAPPDLPTTIPGPVRALVFSCIAKNPSDRPESAAHLARAAQALRRGDTAG
                     ATRQVPAIALADDQRTMLLTQQGDTAATMLLPQGARTTGARPATPPGRPAQGQPAWQP
                     AEQKKRRRRGPWTAPLIALIVILLIVLGGTIWALVNQTGGAKPQAKQTSSAPARTTPA
                     TPSSTPDQYMMITKSDYVGLSFDAAASQLAAAGNTASPSQNEGKPATSPSQVGTVYDV
                     NPTGNVRRTQPIQFTVYSKMAAPSTPGAAPGLSQTTAAPGDTVTVTFGQDSCPSGQTL
                     TAHQLYVQAPGQSTAQGEDKVDVNVSPAVWNVPADAAPGTYQISYTIYCGDTESKPSP
                     SVAVTVTAAATSAPPSSSDSPTPGAGGDHGPGNNG"
     gene            7015..8865
                     /gene="pknB"
                     /locus_tag="VFQ96_03530"
     CDS             7015..8865
                     /gene="pknB"
                     /locus_tag="VFQ96_03530"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU0256892.1"
                     /translation="MNGGGRLVAGRYLVGDLVGRGGMSDVYRGRDTRLGRVVAIKFLK
                     STLADDPEFRARFRREAQAAARMTHPTIVRIFDAGEDDTSDGPGGACSSFIVMEYVVG
                     TALREMIHGNRLDVTAAVRIASGILTALEYSHRADIVHRDIKPGNVMITLAGQVKVMD
                     FGIARAITETATALAQTTTILGTAGYFSPEQARGEVLDARTDLYSTGVVLFEMLTGRP
                     LFTGTTAVDVARQHIRTPAPPARRWNPAVSPLLETVVHRALAKDRDERYQSAREFRLE
                     LQAAHRQGAAATRARLATLAATGTPDPAYPHAPDTPDLASAASDSPVAATSPAEEEPA
                     RATPPAHADGRPPEAPLPARPGPAAPSEPSATPRHRTHAAWLWVTACALALAVVVAFG
                     YWFVEMRPSAGAAATARTVPRLAGLTYEHAAHRLSGMGLTPVRSDEASTAVDPGLVIG
                     TRPHAGASLASGDTVRLLVSSGPPVTAVPDVETMHVDTAEETLTAAGFTPGAVDQQDS
                     SEAPAGTVVRTIPEAGSTQAEGTVVDMVVSSGTVDLPDLVGMELSDATDRLQAPPLGL
                     APRARADPNCQASAPRVVSTQSLPPGEVPQHSVVTLGYCTGGATTPEGGE"
     gene            complement(8879..9517)
                     /locus_tag="VFQ96_03535"
     CDS             complement(8879..9517)
                     /locus_tag="VFQ96_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488828.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU0256893.1"
                     /translation="MTRVLVVDNYDSFVYTLNGYLQELGAKTEVLRNDAFEADEAKDR
                     IADYDAVLVSPGPGTPAQAGVSIPLVRAAADTGTPLLGVCLGHQAIAEAFGATVGVAD
                     ELMHGKTSLIEHDDSDLYAGVAQPFTATRYHSLAVRDDTVPKILDVTARTRDGGVIMG
                     LRHREAPIVGVQFHPESVLTESGYRILGNWLESVGVEGAALRAATRSPLPLA"
     gene            9692..9940
                     /locus_tag="VFQ96_03540"
     CDS             9692..9940
                     /locus_tag="VFQ96_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEU0256894.1"
                     /translation="MARTDGTKKPAQPILAPRRKEDLPNPVWFKPVMFGFMLLGLVWI
                     IVFYVSSSMYPIPGLGSWNILIGFGIAFVGFLMTTRWR"
     gene            complement(10031..10882)
                     /locus_tag="VFQ96_03545"
     CDS             complement(10031..10882)
                     /locus_tag="VFQ96_03545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0256895.1"
                     /translation="MSEPDESRANFCYRHPDRQSFVLCQRCGRTICGECQTPAPVGVI
                     CPECMAEQRASAPRTRSPLVKRTRRLATPGQPAVTYAIMGICILAWLVESLPGIGGHV
                     TNALQYAGVYSYPGPVFQPWRMLTSLAVHYSILHIGLNMYTLWIFGKVLEPVVGRGRY
                     LTLFLVSGFGGSVGVLLLASPLVAVGGASGALFGLFGALIVIHRRLGGNITQLLVLLA
                     INLVISFIPGFNIAWQAHLGGLVIGSLVGWIFIETRGRALFGRQVGLVALLSVVLVAA
                     SFLHFVA"
     gene            complement(10895..>11020)
                     /locus_tag="VFQ96_03550"
     CDS             complement(10895..>11020)
                     /locus_tag="VFQ96_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0256896.1"
                     /translation="FGRVEDEASRAVIDEVAAVPTDSRDRPLDDVVIESVTVEDV"
BASE COUNT         1803 a         4021 c         3600 g         1596 t
ORIGIN      
        1 tcacgccaac acctcgagat cctgtgggag ggcgactccg tcaccgcccg cgacctggga
       61 tcgaccaacg gttctcgtct cgacggccac acgttcaccg agtcggcgct gcgccccgac
      121 gccgtcctga caatcggcca cacccgcatc gtgtaccggc tggtgccgcg ccgaggccgc
      181 gaagaggacg cccgggacgg ccgggcggcc gacccgagcg ctctcggcga tcgagaaccg
      241 tgggagcgcg acgacgtcga ggggccggat tccggcgatc cgttccgcgc gaacccgtac
      301 gccgaagacg ggtccgaaca cgaggggcta gagaacccgt accgcgacga ctcccgccgt
      361 gagggctccg cccccggagg ccgcgagccg tgggagaccg aacctcccga accagagggt
      421 gccggccgcg acgaaggctc gggtgaggaa ggttctggcc gcgagccgtg ggagagcgaa
      481 ccaggagaga gacagtgatc agtgcgctga ctctcctcgt gttgcgcatc gggtttctgc
      541 tcctcctctg gctcttcgtc ttcgccgtcg tctacgccct gcgcagcgac ctgttcggcc
      601 ggcgcgcccg taccctcgac ccagcctcgg ctccccgacc cagcggggcg gcccccagcc
      661 ggccgagcgg cgggcccacg acgatgatcg ccatgacgcc gacgcctgcg ggcgccgcgc
      721 agggcgtggt gatgaagaag caccagccgc tcgcgacgcc cggcgcctcg cccggcacgg
      781 cacccgccgc cacgatcggc gtcgcccgca accttgtcat cacctccggg ccgaagaagg
      841 gcacgacgct ggagctcggg cgagaaccga tcaccatcgg ccgggcccgt gagtcggtgc
      901 tcgtgttacg cgacgactac acctccaccc atcacgcccg catcatggtg tggaacgaca
      961 cctggatgct gcaagacctc ggctccacga acggcacctt cctcgccgga acccgtatcg
     1021 gcacaccgac gatcgtcccg ctggacaccc cgatcaaggt cggcatgacc acgttcgagt
     1081 tgcggcggta ggcggcgagg atgcccgaga tcaaccacgc cgtcgccgtg tctcacgtcg
     1141 gcaaggtgcg cgcgaacaac caagattcgg gctacgcggg ccgcgagctc tttcttgtcg
     1201 ccgacgggat gggcgggcac gccggaggag acgtcgccag cgccctcgcc ctgcggcgca
     1261 tcaaggaggc cgaccggcag tacgccgacg cggccgaggc ggaagacgcg ctgcacgcgg
     1321 cgcttctggc cgcgaacacg agcctcaccg acaaggtcat ggagcggccc gaactgacgg
     1381 gcatgggtac gacggtcagc gccatctgcc gtgtcggcga ccacatggcc gtcgcccaca
     1441 tcggcgactc acgcatctac ctgttccgtg acggggtgct cacccaggtc tccaccgacc
     1501 acacattcgt gcaacgcctc gtcgacagcg gccgcatcac ccgggaggag gcacgggtgc
     1561 acccgcgccg ctccgttttg atgcgcgtct tgggcgacgt cgacgcatcc cccgagatcg
     1621 acaccttttc cgtggcgaca aggcccggcg accggtggct gctgtgctct gacgggttga
     1681 ccggagtgct ccacgacgcg gcgatccgcc gggagctcgc gaccggtgac agtccgcggc
     1741 agaccgcgag ccggttgctg cgtcacacgc tcgacggggg cgcccccgac aacgtgacga
     1801 tcgtgatcct cgacatcgcc gacgacctgc ccggcacacg ttcctccgaa cctgtgacgg
     1861 tcggctctgc ggcagggccg ctcggagcgg aaccggccgc ccccgcgggg cgcaccatcc
     1921 gactgccggc actccggctg cgccccgcgt cgccgttggc cgaccccagt cacttcgagt
     1981 cgcccagcca ggactacctg aacgagctga tcgaagaaga cgcccgacgg gcccgccgcc
     2041 gcaagacctt ctggcttgtg gcggcgatcg tcgtggccct cgccctcgtg gcggccgggt
     2101 tcaccgcata ccggtggaca cagagcatgt attacgtcgg agtgcatgac ggccaggtgg
     2161 tgatctaccg cggggtgcag cagagcgtgg gcccactctc cctctcacac gtttacgagc
     2221 gctccggcat cccggtgtcg ggcctcgact cgttcgatcg ccaacaggtc accgagacgg
     2281 tcaacaccga ctcactgccg gacgcgcggc ggatcgtgga acacctgcgc gacgatacgg
     2341 acagccgatg accgccgagg catgggcgcc cacggcgccc gcccagaccc ggcaccgctg
     2401 gcggctgccc acgcggttgc gcaatctcga gcttctgctg ctggtcttcg cctgtgcgat
     2461 caacggggcg gcgatcctcc tcgtgcagct gggggccctc ggccacgtcg acttcgcgct
     2521 gctgtattac tgcgccgctc tggccgccct cgtctttctg ctccacgtct gcctccgggt
     2581 ttccgcgccg cacgccgacc cgctcatcct gccgatcgtc acggtgctga acgggctcgg
     2641 catcgccgag atctaccgaa tcgacctcga gctcggccag tcgggctggc agagcgccgg
     2701 ggtgcgccag atcgcgtgga cggcgattgc cgtggcctgc gctatcgccg tcatcctcgt
     2761 ggtgcgccac caccgcatgc tgcagggata cacctacctc ttcggcatcg gcgcgatcgt
     2821 gctgctcctc ctgcccatgg tgccggggct cgggcgcacc gtcttcggcg cccgcatctg
     2881 gatcgcggtc ggcccgttca gctttcagcc cggcgaggtc gcgaagcttt tcctcgccgt
     2941 cttcttcgcc ggctacctgg tgcagcatcg cgacgcgctc tcgctcgtcg gccgcacggt
     3001 cttgggcgtg cgactgcccc ggctgcgcga catgggcccg atcctcgtcc tctgggcgct
     3061 ctcgatggcg gtgatcgtct tccagcgcga cctcggcacc gcgcttttat acttcggcct
     3121 gttcctcgtc atgctctatg tcgcgaccgg acgggtcagc tgggtcgcga tcggtgtcgt
     3181 cctcttcgcc ggcggggcgc tggtcgccag ccgcaccctg ccctacgtca cggcccgatt
     3241 ccagaactgg ctcgacccgt tcaacccgca cgtctacgac gccacccgag gcagcttcca
     3301 gctcgtgcaa ggcctcttcg gcatggcata cggagggatc ctcggtacgg gtctcggcca
     3361 gggccggccc gccacggttc cgctggccca aagcgactac atcatcgcga gcctcggcga
     3421 ggagctcggg ctggcaggcc tcttcgcgat cctggcgctc tacctcctct tcgtctcccg
     3481 cgggttccgc atcggattct cggggcgtga cgacttcggc aagctcctcg cggtcgggtt
     3541 ggcgttcacc gtggcactgc aatgcttcat cgtgatcggc ggcataaccc gcgtcatccc
     3601 gctgaccggg cttgcgacgc cgttcctcgc ggcgggcggc tcctccctca ccgcgaactg
     3661 gatcatcgcc gcgctcctct tgcgcctgtc tgacacggtg cacgatcagc cgagggtggt
     3721 ggtgtgaggt gaaccgcgag ctcaagcggg taagcgtcgt cgtgttggcg atgttcgtgg
     3781 cactgctcgt gtcgacgtcg atcatcggcg cggtgcgatc cgattccctg gcggccgatc
     3841 cgcgcaacag cttcacgctg gccgacgccc ggctcgcaca acgcggctcg attctcgtcg
     3901 acggccgagc gattgcgaag tcggtgccga gcaaagacca gtacaagtat cagcgtgtct
     3961 acacgaacgg gccggtgtac tcctccgtca ccggcttctt cccggtgaac ggcgccccga
     4021 ccggaatcga gggcgcgctc aacgagagcc tgtcgggcac cagcggatcg cagttcctca
     4081 cccggctgac cgacaccctg acggggaagc atccgcaggg cgcgtcggtg gagttgacga
     4141 tcgacccgag cctgcagaag gcggcgtacg acgcgctcgg ctccctgaag ggggccgtgg
     4201 tcgtgctcca gcccaagacc gggcgcatcc tcgccatggt ctcccggccc acgttcgacc
     4261 cgaacaccct ggccgggcac aacacggcgc aactcgacgc cacctacaag gctctcctcg
     4321 ccgcgccgac ccggccactc gtcaaccggg cgatcggcgg caacctcaac tatccgggct
     4381 ccacgttcaa accgatcgtc gccgcggccg cgctggactc gggcacgtac accgccgaca
     4441 gcacgctgcc cgacgttgcg gcactgcgcc tgccgggcac cgactcgtac gtgcataatg
     4501 ccgagcgcac gaaatgcggc gacggcaaca cggtgaccat aaccctcgcc atgatccggt
     4561 cgtgcaatat tccctacgcc gaactgggta tgaaactcgg cgccgacacg atccgcgccg
     4621 ccgccgagaa gttcggcttc gggcacgcgt tcgacatccc cataccggtg actcccagca
     4681 tctacccaga cgactacaac gtgccgcaga cggggctcag ctccttcggc caggccagca
     4741 cccgagccac acccctgcag atggcgatgg tgcaggccgc catcgcgaac ggcggcctcg
     4801 tgatgcagcc gaacctcgtc gaatgggtga agtcggcgaa tctgcacacc ctcgaacggt
     4861 tcacgcccaa ggtcttcgac cgcgccatgt ctgagcaaac cgccaacacg ctgcggcaga
     4921 tcatggtaga agacgtcaaa aagggcgtgg ccagtaatgc gacaatagac ggggtcgacg
     4981 tcgggggaaa gaccggcacg tcacagaacg gtccagggct cccctatacg ctgtggttca
     5041 ccggtttcgc gccggcacag agcccgaagt atgccatcgc cgtcgtggtc gaggacggcg
     5101 gcggcgaggg tcagagcggc gtgggaaaca ccatcgccgc gccgattgcg aagaaggtac
     5161 tagaggcggt gctgaagaaa tgagacccac agcagggctc acgttcgggg gacgctacga
     5221 actgcaatcg cgcatcgcca tcggcggcat gggcgaggtg tggcaggcca ccgacagcat
     5281 catcggccgc acggtcgcgt tgaagatcct gaaagacgaa tacctcggcg acccgggatt
     5341 cctggagcgg ttccgtgccg aggcgcggca cgcggcgctc gtcaaccacg aaggcatcgc
     5401 caacgtctac gactacggcg aggaagacgg cagcgccttc ctcgtcatgg agctcgtgcc
     5461 cggcgagccc ctttcggcgg tcctggaacg ggaacacacc ctcaccaccg accgggtgct
     5521 cgacatcgtg gcccagaccg cggccgccct ccacgccgcc cactccgcag ggctcgtgca
     5581 ccgcgacatc aagcccggca acctgctcat cacacccgac gggcgggtga agatcaccga
     5641 cttcggcatc gcgcgcgtcg ccgaccaggt gccgttgacg gcgaccggcc aggtaatggg
     5701 caccgtgcaa tacctctcgc ccgagcaggc gtcgggccac ccggcgtcgc cctccaccga
     5761 catctattcg ctcggcatcg tcgcttacga atgcctggcg ggtcgccggc cgttcaccgg
     5821 cgagtcgcag gtcgcgatcg cgatggctca aatcaacgag gcaccgcccg acctgccgac
     5881 gacaataccg ggccccgtgc gggccctggt cttctcctgc atcgcgaaga acccctcaga
     5941 ccggcccgaa tcggccgcac acctcgcccg agcggcgcag gcgctgcggc gcggggacac
     6001 ggcgggcgcg acccgtcagg tcccggcgat cgccctcgcc gacgatcagc gcacgatgct
     6061 cttgacccag cagggcgaca cggcggcgac gatgctgctg ccgcagggtg cccgaaccac
     6121 cggggcgaga ccggccaccc cgcccgggcg tccggcgcag gggcagcctg catggcagcc
     6181 ggccgaacag aagaagcggc gtcggcgcgg cccctggacg gcaccgctca tcgccctcat
     6241 cgtcatcctc ttgatcgtgt tgggcggcac gatctgggcg ctcgtgaacc agaccggggg
     6301 cgcgaagccg caagccaaac aaacctcctc cgcgccggcg cgcacaacgc ctgcgacccc
     6361 gtcgtcgacg cccgaccagt acatgatgat caccaagagc gactacgtgg ggctgtcctt
     6421 cgacgcggcg gcgagccagc tcgcggcggc cggcaacacg gcatcgccga gccagaacga
     6481 gggaaagccc gcgaccagcc ccagccaagt gggcacggtc tacgacgtca accccaccgg
     6541 gaacgtgcgt cgcacgcagc ccatccagtt caccgtctac agcaagatgg cggcaccatc
     6601 gacgccgggc gccgcccccg gcctcagcca gaccacggcc gcaccgggcg acaccgtcac
     6661 ggtcacgttc gggcaagact cctgcccgtc ggggcagacc ctcactgcgc accagctgta
     6721 cgtgcaggct cccggccagt cgacggccca aggggaggac aaggtcgacg tgaacgtctc
     6781 gcccgccgtg tggaacgtgc cggccgacgc tgcgcccggc acctaccaga tcagctacac
     6841 gatctactgc ggcgacaccg agtcgaagcc ctcgccgtcg gtcgccgtca cggtgaccgc
     6901 cgcggcgacc tccgcgccgc cgtcttccag cgactcgccg acacccggcg cgggcggcga
     6961 ccacggcccc gggaacaacg ggtgacccgc tgacgtgacc tgcgggaagg cttcgtgaac
     7021 ggcggcggcc ggctcgtcgc cgggcgctat ctcgtcggcg acctcgtggg ccgcggcggc
     7081 atgtccgacg tctaccgcgg ccgcgacaca cgcctgggcc gtgtggtcgc catcaagttc
     7141 ctgaagtcga cgctggccga cgacccggag ttccgcgccc ggttccggag ggaagcccag
     7201 gcggcggcgc gcatgacaca cccgaccatc gtgcgcatct tcgacgcggg cgaagacgac
     7261 acgtccgacg gcccaggcgg cgcctgttcg tcgttcatcg tcatggagta cgtggtcggc
     7321 acggcactgc gcgagatgat ccacggcaac aggctcgatg tgacggccgc cgtgcgcatc
     7381 gcctccggca tcttgaccgc gctcgaatac tcacaccggg ccgacatcgt gcatcgcgac
     7441 atcaagcccg gcaacgtgat gatcacgctc gccggccagg tcaaggtcat ggatttcggc
     7501 atcgcccgcg ccatcacaga gacggcgacg gcgttggctc agaccacgac gatcctcggc
     7561 acggccggct acttctctcc cgaacaggcg cgcggcgaag tactggacgc gcgcaccgac
     7621 ctgtactcca ccggggtggt gctcttcgag atgctcaccg gccggccgct cttcacgggc
     7681 accacggccg tcgacgtcgc ccgacagcac atccgcactc ctgcgccgcc cgccaggcgc
     7741 tggaaccccg ccgtatcgcc gctgctcgag acggtcgtgc accgcgcgct ggccaaagat
     7801 cgcgacgaac gttaccagag cgcgcgggag ttccgcctcg agctgcaggc tgcacaccgg
     7861 cagggcgcgg ccgccacgcg ggcgaggctc gccaccctgg ccgccaccgg tacgccggat
     7921 ccggcgtacc cgcacgcccc agatactcca gatctcgcgt cggcggcttc ggattcccct
     7981 gtggccgcca cctctccggc agaagaagaa cccgcccgcg ccacaccgcc cgcgcacgcc
     8041 gacggccgtc cccccgaggc tccactcccg gcacggcccg ggccggccgc accatcggag
     8101 ccttccgcca ccccacggca ccgaacgcac gcggcctggc tctgggtgac ggcatgcgcc
     8161 ctcgccctcg cagtggtggt ggctttcggc tactggttcg tcgagatgcg gccgtcggcc
     8221 ggtgctgcgg ccacggcccg caccgtgccg cggctggcag gcctgaccta cgagcacgcc
     8281 gcacaccggc tctccggcat gggcctcacg ccggtgcgtt ccgacgaggc gagcacggcc
     8341 gtcgaccccg gcctcgtcat cggcacccgg ccgcacgcgg gcgcgtctct cgcttcaggc
     8401 gacaccgttc ggctcctggt ctcaagcggg ccgccggtca ccgcggtgcc cgacgtcgag
     8461 acgatgcacg tcgacaccgc ggaagagacc ttgaccgccg cagggttcac gccgggtgcg
     8521 gtcgaccagc aagattcttc ggaggcgccc gccggcaccg tcgtgcgcac cattcccgag
     8581 gcgggcagca cgcaggccga gggcaccgtc gtcgacatgg tcgtctcgag cggcaccgtc
     8641 gacctgcccg acctcgtcgg catggagctt tccgacgcga ccgaccggct ccaggcgccc
     8701 cctctcggcc tggctccgcg cgcgcgggcc gacccgaact gccaggcgag cgccccccgg
     8761 gtcgtgtcga cccagtcgct gccgcccggc gaggtcccgc agcattccgt cgtgacgctc
     8821 gggtactgca cgggaggcgc gacgacgccg gagggcggcg agtgaggccg gccgaccctc
     8881 aggccaaggg cagcgggctg cgggtcgcgg cacgcagggc cgcgccctcg acgccgaccg
     8941 attcgagcca gttgccgaga atgcgataac cgctctcggt gagcaccgac tccgggtgaa
     9001 actgcacgcc gacgatcggc gcctcccggt gccgtaaccc catgatcacg ccaccgtcgc
     9061 gcgtgcgtgc cgtgacgtcg agaatcttcg gcaccgtgtc gtctcgcacc gccagcgaat
     9121 ggtagcgggt cgcggtgaag ggctgcgcga cccccgcgta gaggtcgctg tcgtcgtgtt
     9181 cgatgaggga ggtcttgccg tgcataagct cgtcggcgac gccgaccgtg gcgccgaacg
     9241 cctcggcgat cgcctgatgc ccgaggcaga cgcccagaag cggggtgccc gtgtcggcag
     9301 ccgcccgcac gagagggatc gagacgccgg cctgcgccgg ggtcccggga cccggcgaga
     9361 cgagcaccgc gtcgtaatcg gcgatgcgat ccttcgcctc gtcggcctcg aaggcgtcgt
     9421 tgcgcagcac ctcggtcttc gcaccgagct cttgcaggta gccgttcagc gtgtagacaa
     9481 agctgtcgta gttgtcgacg acgagaacgc gggtcataga gccaccatgc cttgggggtg
     9541 ccagagaatc ccgaaccgcc tgggcgggcg gcgcggaagg gatgcggaac gcctcctatc
     9601 ctagggtcgg ggcaccggca cggaccggcc cgccccggca gaggcctccc ggccgctgcc
     9661 ggggaagagg gcaccggtag actgtggtgt catggcacgc accgacggca cgaagaaacc
     9721 cgcgcagccg attctggcgc cccggcgcaa agaagacctg cctaacccgg tctggttcaa
     9781 gccggtcatg ttcggtttca tgcttctcgg cctggtttgg atcatcgtct tctatgtcag
     9841 cagcagcatg tacccgattc ccgggctcgg cagctggaac attctcatcg ggttcggcat
     9901 cgccttcgtg ggcttcttga tgacgacccg ctggcgctga ggtcccgcgc tcgcgccacg
     9961 ggaccctccc ggactacagc cgtgtaacta tccccagctg tgtacaagcc tgtggataac
    10021 ggcccggccc tcaggcgacg aagtgcaaaa agctcgccgc gacgagcacc accgacagga
    10081 gcgcgacgag gccgacctga cggccgaaga gcgctcgccc gcgggtctcg atgaagatcc
    10141 atcccacgag ggagccgatg acgagaccgc cgaggtgggc ctgccaggcg atgttgaacc
    10201 ccggtatgaa gctgatcacc aggttgatcg ccaagagaac gagcaattgg gtgatgttgc
    10261 cgcccagcct gcggtgtatg acgatcagcg ccccgaacag gccgaacagg gccccggagg
    10321 ccccgcccac cgcaaccagg ggcgaggcga gcagcaacac gccgacggac ccgccgaagc
    10381 cgctcaccaa gaagagcgtg aggtaacgac cgcgccctac cacgggctcc aacaccttgc
    10441 cgaagatcca cagggtgtac atgttcagcc cgatgtgcag gatcgagtaa tgcacggcga
    10501 ggctggtcag catgcgccac ggttgaaaga ccgggcccgg gtacgaatag acccccgcat
    10561 actgcagtgc attcgtcacg tgcccgccga tgcccggcag cgattcgacg agccaggcga
    10621 gaatgcagat gcccatgatc gcgtacgtca cggccggctg gccgggcgtt gccagccgcc
    10681 tggtgcgctt caccaggggg gagcgcgtcc tgggggcgga ggcacgctgc tccgccatgc
    10741 attcggggca gatcaccccg accggcgccg gggtctggca ttccccgcag atcgtgcgcc
    10801 cgcaccgctg gcagagcacg aaactctgcc ggtcaggatg ccggtaacag aagttcgctc
    10861 gcgactcgtc gggctcgctc acggggaggg gccgtcagac gtcctcgacg gtgacggact
    10921 cgatgaccac gtcgtccagc gggcggtccc gggagtcggt cggcaccgcg gcgacctcgt
    10981 cgatgacggc gcgcgaggcc tcgtcttcga cgcggccgaa
//
LOCUS       DASTNL010000113         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1008567, whole genome shotgun sequence.
ACCESSION   DASTNL010000113 DASTNL010000000
VERSION     DASTNL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008567"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1051
                     /locus_tag="VFQ96_03555"
     CDS             53..1051
                     /locus_tag="VFQ96_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0256897.1"
                     /translation="MPYTDMPLEELRAFTPTVREPADFDAFWSKTLTESRSAAKEGGA
                     AATRVAAQTPITEIVVEDLTFPGFGAEPVKAWVTRPKGREGERLPTVVEFNGYNGGRG
                     IAGERAQWALCGYVHVFMDTRGQGSGWGSGGETADPHGSGAAVAGWMTRGIASPHEHY
                     YRRVYTDAVRLLDEVKTWSFVDPARIAVAGGSQGGGIALAAAGLSGGLWAAMPDVAFL
                     CAWEYGAAVSINGPFGEVARYLSVHRDEADRVWNTAAYFDGVNFAKRITIPALFSVAL
                     MDETVPPSTVFAAFNALASADKAIEVYPYNNHEGGQTYQWLKQVAFLAERSAPAPR"
     gene            complement(1085..1675)
                     /gene="dcd"
                     /locus_tag="VFQ96_03560"
     CDS             complement(1085..1675)
                     /gene="dcd"
                     /locus_tag="VFQ96_03560"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU0256898.1"
                     /translation="MLLSDRDIKAGIQRGRIRLDPYDAQMVQPASVDVRLDRLFRLFS
                     NHKYGVIDPAEDQPELTYLVDVDADRPFILHPGEFVLASTYEKVGLPDDVAARLEGKS
                     SLGRLGLLTHSTAGFIDPGFTGHVTLELSNVATLPIKLWPGMKIGQLCFFQLSSPAEQ
                     PYGAANGSRYQGQRGPTASRSHIGFHRTVVAPLDER"
     gene            1873..1943
                     /locus_tag="VFQ96_03565"
     tRNA            1873..1943
                     /locus_tag="VFQ96_03565"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1905..1907,aa:Gly,seq:ccc)
BASE COUNT          368 a          704 c          723 g          338 t
ORIGIN      
        1 gcgcccgggg tcgcccgcct gacgggcagg ccggccctct taggatcgat gtgtgcccta
       61 caccgatatg ccgctggaag agttgcgcgc gttcactccg acggtgcgtg agcccgccga
      121 tttcgacgca ttctggtcca agaccctcac cgagtcgcgt tcggcggcga aggagggcgg
      181 tgcggccgca acccgcgtag cggcgcagac tccgatcacc gagatcgtcg tcgaagacct
      241 caccttcccg ggcttcggcg cggagcccgt gaaggcctgg gtcacgaggc cgaaggggcg
      301 ggagggcgaa cgactgccca ctgtcgtgga attcaacggc tataacggcg ggcgcggcat
      361 cgccggggag cgtgcacagt gggcgctgtg cggctacgtt cacgtcttca tggacacccg
      421 cggccagggc tccgggtggg gcagcggggg cgagacggcc gacccgcacg gctccggagc
      481 ggccgtcgcc gggtggatga cccgcggcat cgcgagcccg catgagcact actaccggcg
      541 cgtctacacc gacgccgtgc ggctcctcga cgaggtgaag acctggagct tcgtcgaccc
      601 ggcccgcatc gcggtcgccg gcggcagcca gggcggcggt atcgcgttgg cggcggcggg
      661 gctgtcggga gggctctggg ccgccatgcc cgacgtggcc ttcctctgcg cctgggaata
      721 cggcgccgcc gtgtcgatca acgggccgtt cggggaggtt gccagatacc tctccgtgca
      781 ccgcgacgag gccgatcggg tgtggaacac cgccgcgtac ttcgatggcg tcaacttcgc
      841 gaagcgcatc accatccccg ccttgttctc cgtcgccctc atggacgaga ccgtgccgcc
      901 gtcgacggtc ttcgcggcct tcaacgctct cgccagcgcc gacaaggcca ttgaggtgta
      961 cccctacaac aaccacgaag gcggccaaac ctaccagtgg ctcaagcagg tcgccttcct
     1021 cgccgagcgc tccgccccgg ctccgaggtg acatgagccc gcagacccgg gcgcagcggc
     1081 acgatcagcg ctcgtcgagc ggggccacga cggtgcggtg aaagccgata tgcgagcggg
     1141 aggcggtcgg cccgcgttgc ccttggtagc gggaaccgtt cgccgcacca tatggctgct
     1201 cggcgggtga cgagagttga aagaagcaca gctggccgat cttcatgccc ggccacagct
     1261 tgatcggcag cgtcgccacg ttgctgagtt ccaatgtgac atggccggtg aatccggggt
     1321 cgatgaagcc ggccgtggag tgggtcagaa ggcccagccg gccgagcgag ctcttgccct
     1381 ccaggcgcgc cgcaacatca tcgggcaggc cgaccttctc gtaggtggaa gccagcacga
     1441 actcgcccgg atgcagaatg aacggccggt cggcgtcgac atccacgagg taagtcagtt
     1501 cgggctgatc ttccgccggg tcgatcacgc catatttatg gttcgagaag agtcggaaaa
     1561 gccggtcgag ccgcacatcc acgctggccg gctgcaccat ctgtgcgtcg tatgggtcga
     1621 gcctgatgcg cccgcgctgg atgcctgcct tgatgtctcg atcggagagc agcacgccct
     1681 cagcctagtt gagcccgagc caccccttgc cagccgcgcg tcacgcgcca tcggccaaac
     1741 cgccaccggc caccggccaa gtagtgacag ggccggaagg aaacgtcggc ggcttacgag
     1801 cagaaacgtg cgaccgatgc tacctgggcc taggtggaag agacacgcgg ctcccggtaa
     1861 ggttgtgggc acgcgagtgt agttcaatgg cagaatctca gcttcccaag ctgatggcgc
     1921 gggttcgatt cccgtcactc gctctccgat aaaaccccag gtcacagcgt gattccgggg
     1981 tagcgtcggc agtgaggcga aggcgagcta agcccctttc gtgtactccc gtgtactcac
     2041 gggttgacga gagggcatta tcgtggccga aaccacctcc ctactagacc cattcgagcc
     2101 agcgagggcc gcactgtgac ggcccgtaag cgc
//
LOCUS       DASTNL010000114         5697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1565711, whole genome shotgun sequence.
ACCESSION   DASTNL010000114 DASTNL010000000
VERSION     DASTNL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565711"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFQ96_03570"
     CDS             <1..842
                     /locus_tag="VFQ96_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0256899.1"
                     /translation="ILLGPVVGSVLLVGLGPTYGIFTNIIFYVPLTVFLATTAFTGHS
                     RDGSAGTAQRMSLLSSFRVLGTVRRNRKILGMLILAALAAVTIGAVLQTAMPVFGNKL
                     AGGADPDLVYGILLFALGAGGVIGGFFLEASGWIKPTGATAVVSTVAFGLFSALFAFT
                     GSIAVAIAALILTGFAQITSTATEQAIVQLEAPVAERGRVIGAYSMFGPGMQTFSGIT
                     VGVVGSLLGIPLTVIAGGAVLVVGGIATAVYMAGGKKGAPHGAGIAGGTGSEAAYSDL
                     SPE"
     gene            complement(818..1624)
                     /locus_tag="VFQ96_03575"
     CDS             complement(818..1624)
                     /locus_tag="VFQ96_03575"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEU0256900.1"
                     /translation="MSTQNPFPSRARRAIRRVPHGDIVASFATYREAQQAVDGLVKAE
                     FPVEQVAIVGSDLKTVERVTGRLTWGRVALAGAASGAWLGIFLGVLFSIFSAGASVGI
                     IGAAVMIGAGFGMLFGVASYAINRRRKDFTSMTQVLADRYDLIVDPQFGGRAGNLLAE
                     AGVRSTASAPRPGVAETLPSATGVAGPPRGPDRRPRPRYGEYVSEPPAQAAGQEAAGT
                     GSTADGPGDGTGAPPEDDGDTPRGDAGADDGRAAEGGAGAGKDYSGERSE"
     gene            1694..3049
                     /locus_tag="VFQ96_03580"
     CDS             1694..3049
                     /locus_tag="VFQ96_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0256901.1"
                     /translation="MSSARVFAARLAGCLVFDPVGDRVGKVRDVLVVYRRNDPPRVVG
                     LVVEIPGKRRVFLSIGRVTSIGAGQVITTGLINVRRFLQRGGEVRIVAEMLGRTITFR
                     DGSGEGTIEDVAIERSAQGEWAIGQLFVRRPKTSAAPFVKGPTVFAHWNEVRERGRLG
                     KAQSAQQLIATFSDLKAADLANTLRDLPDLRMHEVVRELSDDRLADALEEMPTADQLQ
                     ILARFDDDRAADVLDQMQPDDAADLIAQLQETRRERLLQLMEPKEAEDVRFLLNYAPD
                     TAGGLMTTEPIIVSADATVAEGLALIRRHDLPAALGTTVCVTLPPYDPPTGRFLGIVH
                     FQRMLRYPPHERLSTLIDQTLDPVSVDASAAEVTRTLASYDLVAVPVVDDNHRLVGVV
                     TIDDILDYLLPDDWRNTDGEAGDGDRAVAQLKTATGQTALGPVTGEADGPGGKGVGDA
                     R"
     gene            3039..3581
                     /locus_tag="VFQ96_03585"
     CDS             3039..3581
                     /locus_tag="VFQ96_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU0256902.1"
                     /translation="MHARKAGDLLDAPRGLRAPLFSRRAPRGEGDTFGRFTEWIARAM
                     GTPWFLLGLTLFSAIWISWNTLLPAPWRFDSAKLGFIALTLVLSLQASYAAPLILLAQ
                     NRQDDRDRVQIEQDRQRAERNLADTEYLAREVVALRLAIQDVATRDFVRAELRSVLAE
                     LDAARHEGESGAQADEPTGD"
     gene            complement(3645..4088)
                     /locus_tag="VFQ96_03590"
     CDS             complement(3645..4088)
                     /locus_tag="VFQ96_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU0256903.1"
                     /translation="MLSGLSLDKLLIVGVIAVLLLGPTRLPSYAKKLAVLVKRLRAFA
                     SDAQDRMREEMGPGFDDVDWKKLDPRQYDPRRIIREALFDDGDDDADQVRTGISAPSD
                     DAAQALADDEGTADDTGAATGGETPVDAAADEAMVSVPPFDQEAT"
     gene            4209..4838
                     /locus_tag="VFQ96_03595"
     CDS             4209..4838
                     /locus_tag="VFQ96_03595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU0256904.1"
                     /translation="MSKTSSWKFAGELVVEDDILTTARTQSLELGIEAIEPAVGAQAR
                     LVVAASRARSIIEIGTGAGVSGLWLLGAGGEASLTSIDVELDFQQHAREYFSRAGVHG
                     NRVRLITGRALDVLPRMNENSYDIVFVDADPRQVIECFEHALRLARQGGIILVPHALW
                     KDRVAEPARRDAVTAAFRTLLAETAVSDAVTTAVSPAGDGLLQVIKKGA"
     gene            complement(5023..5196)
                     /locus_tag="VFQ96_03600"
     CDS             complement(5023..5196)
                     /locus_tag="VFQ96_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEU0256905.1"
                     /translation="MAAMKPRTGDGPMEAVKEGRLIIVRVPLEGGGRLVVSVNDDEAK
                     ELHDALATAAGIA"
     gene            complement(5309..>5697)
                     /locus_tag="VFQ96_03605"
     CDS             complement(5309..>5697)
                     /locus_tag="VFQ96_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256906.1"
                     /translation="EAPLVPFAPWFEAAAWWSKMVHAPVWVGLATLTAAIVLFMLVLL
                     LPAVRRLGLEVRLWLASYAGYLLAVFFPQSSTFRLLVPLFPLAGPLAAPRSTTYRVVL
                     VAAFLAAQAGWLALCWGVDGADWSPP"
BASE COUNT          881 a         2060 c         1906 g          850 t
ORIGIN      
        1 gcatcttgct gggcccggtc gtcgggtcgg tgcttctcgt aggcttaggg ccgacctacg
       61 gcatcttcac gaacatcatc ttctacgttc cgctcaccgt cttcctcgcg acgaccgcgt
      121 tcaccgggca cagccgagac ggctccgcag gcaccgcgca gcgcatgtcg ctcctctcct
      181 cgttccgggt gctcggcacg gtgcgccgca accgcaagat tctcggcatg ctcattctcg
      241 ccgcgctggc cgccgtcacc atcggggccg tgttgcagac cgcgatgccg gtcttcggga
      301 acaagctcgc gggtggggcc gaccccgacc tcgtgtacgg catcctgctg ttcgccctgg
      361 gcgcgggcgg cgtgatcggc gggttcttct tggaggcgag cggctggatc aagcccaccg
      421 gcgccacggc cgtcgtcagc accgtcgcgt tcggcctgtt ctcggccctc ttcgcgttca
      481 ccgggtcgat agccgtcgcg atcgcagcgc tcattctcac gggcttcgca cagatcacga
      541 gcacggcgac cgagcaggca atcgtgcaac tggaggcccc ggtcgccgag cgtggccgcg
      601 tgatcggcgc atacagcatg ttcggccccg gcatgcagac cttcagcggc atcaccgtcg
      661 gcgtggtcgg cagcctcctc ggcatacctt tgaccgtcat cgcgggcggc gcggtgctgg
      721 tcgtcggagg catcgcgacc gcggtctata tggcgggtgg caagaaaggc gcgccgcacg
      781 gtgccggcat tgccggcggc accggttcgg aggcggccta ctccgatctc tcccccgagt
      841 agtccttccc cgcgccggcc ccgccctcgg cggcacggcc gtcgtcggcg cccgcatcgc
      901 ctcgcggggt atccccatca tcctctggcg gggcgccggt gccatccccg gggccgtctg
      961 cggtactccc cgtgccggcg gcctcctggc ccgcggcctg cgcgggcggc tcggagacgt
     1021 attcgccgta tcgcggccgg ggccgtcggt cggggccgcg gggcgggccc gcgacgccgg
     1081 tggccgacgg cagggtctca gccacgccgg ggcgcggcgc cgacgccgta ctgcgcacgc
     1141 ccgcctcggc gagaagattg ccggcacgac cgccgaactg cgggtcgacg atcaggtcgt
     1201 agcgatcggc caacacctgc gtcatcgacg tgaaatcttt gcgacgccgg ttgatggcgt
     1261 acgaggcgac gccgaagagc atcccgaaac ccgcgccgat cattacggcg gcaccgatga
     1321 tgccgacact cgcgccggcg gagaagatgc tgaagagcac gccgaggaag attcccagcc
     1381 acgcgcccga cgccgcgcct gcgagagcga cccggcccca ggtgagtctg ccggtcaccc
     1441 gttcgaccgt cttcaggtcg ctgccgacga tcgcgacctg ctcgacgggg aactccgcct
     1501 tgacgagccc gtccaccgcc tgctgcgcct cccgataggt ggcgaacgac gcgacgatgt
     1561 cgccgtgagg cacccgacgg atcgcccggc gggcgcgact cgggaacggg ttctgggtgc
     1621 tcatccagca cacgttaccc gcacgccgcg cggccggaca cgggtgcggc gcacgacacc
     1681 gtaagttatc agtgtgagtt ccgccagagt gttcgccgca cggctcgccg gctgcctcgt
     1741 cttcgacccg gtcggcgacc gggtcggcaa ggtgcgcgac gtgctcgtcg tgtatcggcg
     1801 caacgaccct ccacgggtgg tcggcctcgt cgtcgagatc cccggcaaac gtcgcgtttt
     1861 cctgtcgatc ggccgcgtga cctcgatcgg cgccggccag gtcatcacga ccgggctgat
     1921 caacgtgcgg cggttcctgc aacgcggcgg cgaggtgcgg atcgtcgcag agatgctggg
     1981 gcgcaccatc acgttccgtg acggctccgg cgaaggaacg atcgaagacg tggcgatcga
     2041 acgttccgcg cagggcgagt gggcgatcgg gcagctcttc gtgcggcgcc cgaagacgag
     2101 cgccgcgccg ttcgtcaaag gcccgaccgt cttcgcacac tggaacgagg tgcgcgagcg
     2161 cgggcgactc ggcaaggccc aatcggcgca gcagctgatc gcgacgttct ccgacctcaa
     2221 ggcggccgac ctcgcgaaca ccctgcgaga cctgccggac ctgcgcatgc acgaggtggt
     2281 gcgagagctc tccgacgacc ggctggcgga cgcgctggag gagatgccga cggccgacca
     2341 gctgcagatc ttggcacgct tcgacgacga ccgggccgcc gacgtgctcg accagatgca
     2401 gcccgacgac gcggcagacc tcatcgcaca gctgcaggag acccgacggg aacgcctgct
     2461 gcagctgatg gagcccaaag aggccgaaga cgtgcgcttt ctcttgaatt acgcccccga
     2521 cacggcaggc gggctgatga cgaccgagcc gatcatcgtc tcggccgacg cgaccgtcgc
     2581 ggaaggcttg gccctcatcc gccgccacga cctgcccgcc gcgctcggca cgacggtctg
     2641 cgtcaccctg ccgccgtacg acccgcccac gggccgcttc ctcggcatcg tgcactttca
     2701 acggatgctg cgatatccgc cccacgaacg gctcagcacg ctcatcgatc agacgctcga
     2761 cccggtgagc gtcgacgcct ccgccgcgga ggtcacgcga accctcgcca gctacgacct
     2821 cgtcgcggtg ccggtggtcg acgacaacca ccggctggtc ggtgtcgtca cgatcgacga
     2881 catcctcgat tacctgctgc cggacgactg gcgcaacacc gacggcgagg ccggcgacgg
     2941 cgaccgggcg gtcgcgcagc tgaagaccgc caccgggcag acggcgctcg gcccggtcac
     3001 cggcgaggcc gacgggccgg gaggtaaggg ggtgggcgat gcacgctagg aaggcaggcg
     3061 acctgctcga cgcgccccgc ggccttcgcg ccccgctctt ctcgcggcgt gcgccccgcg
     3121 gcgagggcga cacgttcggc cggttcaccg agtggatcgc ccgggcgatg ggcaccccct
     3181 ggttcctcct cggcctgacg ctcttctcgg cgatctggat ctcgtggaac acgctgctgc
     3241 cggcgccgtg gcgcttcgat tcggccaagc tcggcttcat cgcgctgacc ctggtgctca
     3301 gcctgcaggc ctcctacgcc gccccgctca tcttgctggc gcagaacagg caagacgacc
     3361 gcgaccgggt gcagatcgaa caagaccggc aacgcgccga acgcaacctc gccgacaccg
     3421 aatacctggc ccgcgaggtc gttgcgttgc gcctggccat ccaagacgtg gcgaccaggg
     3481 atttcgtgcg cgccgaactg cgctcggtgt tggcggaact cgacgccgcc aggcacgaag
     3541 gggaatcggg cgcccaagcc gacgagccga cgggcgacta gcttcctccg ccccggcctg
     3601 ccatgcggtc ctgtcgtaag cgccggccga cgggccgtcg tctgtcaggt cgcctcttga
     3661 tcgaacggcg gcacgctcac catggcctcg tcggccgccg catccacggg cgtctcgccg
     3721 ccggtcgcgg cacccgtgtc gtcggccgtt ccttcgtcgt cggcgagggc ctgcgccgcg
     3781 tcgtcgctcg gtgcgctgat accggtacgc acctggtcgg cgtcgtcgtc cccgtcatcg
     3841 aaaagcgcct cgcggatgat gcgccgaggg tcgtattggc gggggtcgag tttcttccag
     3901 tcgacatcat cgaagcccgg gcccatttcc tcccgcatgc gatcttgagc gtcgctcgcg
     3961 aacgcacgga ggcgtttcac cagcacggca agcttcttcg cataggaggg caagcgcgtc
     4021 gggccgagca gcagcaccgc gatgacgccg acgatcagca acttgtcgag actgagccca
     4081 ctgagcaccc ccacagacta cccgtgccga tcaggtgacg accgcatgcc tgccggtttc
     4141 ccgcccagaa cgccaagcag cgacccggcg caccgtatcc ttatggtgct gaagagacgg
     4201 gaggcacggt gtcgaagacc tcgagctgga aattcgccgg ggagcttgtc gtcgaagacg
     4261 atatcctcac cacggcccgt acgcagtctc tcgaactggg catcgaggcg atcgagccgg
     4321 cagtcggcgc acaggcccgt ctcgtcgtcg ccgcctccag ggcccggtcg atcatcgaaa
     4381 tcggcacggg ggcgggagtc tcgggcctct ggctgttggg ggcgggcggc gaggcctcgc
     4441 tcacctcgat cgacgtcgaa ctcgattttc agcagcacgc gcgagagtac ttctcccggg
     4501 cgggggtgca tggcaaccgc gtccgcctca tcaccgggcg ggcgctcgac gtgctgccca
     4561 ggatgaacga gaactcgtac gacatcgtct tcgtcgacgc cgatccccgg caggtcatcg
     4621 aatgcttcga gcacgcgtta cggctggccc gccagggcgg catcatcctc gtcccgcacg
     4681 cgctgtggaa agaccgcgtg gccgagccgg ccaggcgcga cgcggtcacg gcggccttcc
     4741 gcaccctcct cgccgagacc gccgtctccg acgccgtcac gaccgccgtc tcgccggccg
     4801 gcgacggcct gctccaggtc atcaagaaag gcgcctgagc tgaggctttc cggcgatccg
     4861 aagccttccc acggctccga ggctaccgga ggcgccgagc ctcccggcag ccccgagccc
     4921 cctcggcggc acggtgtcgg ccgaaaagag agccggcgcc ctgccgaact cgcgttcggt
     4981 cagaggcgcc ggctcgtacg atgtgtcgcc gcgcggtccc cgctaggcga tgcccgccgc
     5041 ggtcgcgagc gcgtcgtgaa gctccttcgc ctcgtcgtcg ttgaccgaca cgacgagtcg
     5101 cccgccaccc tcgagtggta cacggacgat gatgaggcga ccctccttca ccgcctccat
     5161 cggcccgtct ccggtgcgcg gcttcatggc tgccatcagg tcccctttcg tgagtcttca
     5221 cccattatct caatttcgac cgggcccacg aaaaccgtgg cggggtgtga cgtgcccgag
     5281 gccacggccg ataccggccc gcggcgggtc acggcggtga ccagtcggct ccgtccactc
     5341 cccagcacag cgcgagccag ccggcctgtg cggcgagaaa tgcggcgacg agcacgaccc
     5401 ggtacgtcgt gctacgcggc gcggcgagcg gccccgcaag gggaaagagc ggcacgagga
     5461 ggcggaaggt gctcgactgc ggaaagaaga ccgcgagaag atagccggca tacgaggcca
     5521 gccacagcct gacctccaga cccagcctcc gcaccgccgg cagcagaagc acgagcatga
     5581 agagcacgat ggcggccgtg agcgtcgcaa gccccaccca taccggggcg tgcaccatct
     5641 tcgaccacca tgcggcggcc tcgaaccacg gcgcgaacgg cacgagaggt gcctcgc
//
LOCUS       DASTNL010000115         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_640457, whole genome shotgun sequence.
ACCESSION   DASTNL010000115 DASTNL010000000
VERSION     DASTNL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640457"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFQ96_03610"
     CDS             complement(<1..191)
                     /locus_tag="VFQ96_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256907.1"
                     /translation="MPGAVSIELGASAGRVAGVEGLVLMMCGHGAMSAAGILQGAGIN
                     GTYVFDGSPEQWARSTTAPG"
     gene            325..1035
                     /locus_tag="VFQ96_03615"
     CDS             325..1035
                     /locus_tag="VFQ96_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0256908.1"
                     /translation="MDSIGENGADVGDRQLKSEMFDQLASVGKALANGKRLELLELLA
                     QGERGVDALAQAAGLGLSTASAHLQLLRRAGLVVTRREGTSIHYRLAGWDVARLLQSL
                     CTIAESHAPAVEKARAAYLNTRPHPAHDAPEISRQELMNMADDAAVTVLDVRPEEEYA
                     AAHIPGALSIPVEELAARIAEVPAGRQVVAYCRGAYCVLAYEAADTLHAAGRPARRLH
                     GGMLEWRANDLPTESSAA"
     gene            complement(1223..1295)
                     /locus_tag="VFQ96_03620"
     tRNA            complement(1223..1295)
                     /locus_tag="VFQ96_03620"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1259..1261),aa:Glu,seq:ctc)
     gene            1420..2061
                     /locus_tag="VFQ96_03625"
     CDS             1420..2061
                     /locus_tag="VFQ96_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0256909.1"
                     /translation="MHLLKLEHSALVVSNGTGTLYVDPGSFTRPVPVPNGPAAVVISH
                     EHADHWTPQQLDRILGGASGPVPIIGPEGVARAAHGYDVTVARAGESLEASGFTLRFF
                     GGRHAVIHRSIPVVDNLAVLVDGLLFHPGDSFTVPDDTRVDTLAVPASAPWLKASEFM
                     DYVLAVRPKRSFAMHECINSEAGNRMAFERIKTTTEAGGGTFLPLRAGDETDL"
     gene            complement(2137..2400)
                     /locus_tag="VFQ96_03630"
     CDS             complement(2137..2400)
                     /locus_tag="VFQ96_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256910.1"
                     /translation="MSSRQTRAPRKFTDRRAEQLYHAARLSRLQAAFPWSAALPVAER
                     ERFGQELSEHSARTSDRELDRLLAGWKARARAYERRARPRRAA"
     gene            complement(2547..2957)
                     /locus_tag="VFQ96_03635"
     CDS             complement(2547..2957)
                     /locus_tag="VFQ96_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0256911.1"
                     /translation="MPEFRSISHLDLSVSDVARSAAWYVETLGLKQLSRSDLDNRIMV
                     VLLHRETGLVIGLNQHRQSAADRFDERRPGLDHVGFAVAKREDLDAWEARLTELGVEH
                     SPVTDSPSGSGTALVFRDPDNIQLEFWWSRPRKL"
     gene            complement(3038..3682)
                     /locus_tag="VFQ96_03640"
     CDS             complement(3038..3682)
                     /locus_tag="VFQ96_03640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CE1759 family FMN reductase"
                     /protein_id="HEU0256912.1"
                     /translation="MSARRLAAVGAGLATPSSTRMLVDRLVEATLDQLQDHGIEAGAD
                     VFELRDYAHDVTDNMLAGFASPKLESMINAVVSADAVIFATPIFSTSYSGLFKSFIDV
                     LDKEALLDIPVLLAATAGTPRHSLAIDYAMRPLFAYLHADPVSTAVFAASADWGGSGD
                     QVATLAERIRKGATELAAKVARRDPASVSDPFDPDTYLGKGHSFGNLLGGLAGE"
     gene            complement(3679..>4660)
                     /locus_tag="VFQ96_03645"
     CDS             complement(3679..>4660)
                     /locus_tag="VFQ96_03645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CE1758 family FMN-dependent luciferase-like
                     monooxygenase"
                     /protein_id="HEU0256913.1"
                     /translation="TTLITTSDPVKVAEDFAVLQHVSGGRADLMLGRGNTGPVYPWFG
                     KDIRQGLELAVESYALLHRLWREDVVDWSGRFRTPLQGFTSIPRPLDGVPPFVWHGSI
                     RTPEIAEQAAYYGDGFFANNIFWPAEHYQRLIELYRQRFAHYGHGTPEQAIVGLGGQA
                     FMRKNSQDALDEFRPYFDNAPVYGHGPSMEDFTKMTPLTVGSPQQVIDRYAGMRDLFG
                     DYQRQLFLLDHGGLPLKTVLEQIDLLGGEVVPVLRRELAQGRPATVPDAPTHASLVRA
                     QYGDGPVRQSRPNANRGDNLTAGASPYEASVTAGEAESAEAGRGAAEAVR"
BASE COUNT          780 a         1618 c         1545 g          717 t
ORIGIN      
        1 cccggggccg tggtgctgcg tgcccactgc tcggggctgc catcaaacac gtaggtgccg
       61 ttgatgccgg cgccttgcag gatgccggcg gcgctcatcg ccccgtgccc gcacatcatc
      121 agcaccagcc cctcgacacc ggcgacccgt ccggccgagg cgccgagttc gatactcacg
      181 gcgccgggca catggcccat ccccctccga ctcttcccca acggcggccg ccacgggacc
      241 tttcgcgccc gcaaacctcg ctcagggctc acttcgctac taattatcca ttaatacatg
      301 gactaataga atggttgatg gagtttggat tccatcggcg agaacggggc agacgtgggc
      361 gacagacagc tcaaatcgga gatgttcgac cagcttgcaa gcgtgggaaa ggcactggcg
      421 aacggcaaac ggcttgagct actcgaactg ctcgcccagg gcgaacgcgg cgtcgacgcc
      481 cttgcccagg ccgcgggctt gggcctcagc acggcctctg cccacctgca gctgctgcga
      541 cgcgccggcc tcgtcgtcac caggcgagag ggaacgagca tccactaccg cctggccggt
      601 tgggatgtcg cacgacttct ccaaagcctg tgcaccatcg ccgaaagcca cgcgccggcc
      661 gtcgagaagg cacgggcagc ctacctgaac actcgacccc accctgccca cgacgccccc
      721 gaaatcagtc gacaagagct catgaacatg gccgacgacg cagccgtcac tgtgctcgac
      781 gtgcgtccag aagaggaata cgccgctgcc catattcccg gcgccctgtc catccccgtc
      841 gaagagttag ctgcccgtat cgctgaggtg ccggcgggcc gccaagtcgt cgcctactgc
      901 cgcggcgcat actgcgtgct cgcctacgag gccgctgata ccctgcatgc cgccgggcgg
      961 cccgcccgcc gacttcacgg cggcatgctc gaatggcggg cgaacgacct ccccacagag
     1021 agcagcgccg cctgaacacg ctccgcccgg aaacacccac gaccgtccag gatccgaggg
     1081 cgacccgaca gtgtcgccac ggcgctcaac ctcgccgcga ggcaaccaga cgttgccggt
     1141 ctgttgccaa aagccgcctg gcgccctggc agtcttaccc acaaagagta gaaccccggt
     1201 ctgaccggga gttttactat cgtgacccca gcgagatttg aactcgcgtt accgccgtga
     1261 gagggcgacg tcctaggccg ctagacgatg gggccgtaca acccattgag tatgccacag
     1321 cggcaccggg caggccaaac tgcgagggcc gccgccggac ggagccgggg ggccgcgcgc
     1381 ctggttgcag gccggccgca cgcggtgttg gctcaaggaa tgcaccttct gaagctcgaa
     1441 cactcggccc tcgtcgtctc gaacggaacc gggacccttt acgtcgaccc cggctcgttc
     1501 acccggccgg tgccggtgcc gaacggccct gcagcggtcg tcatcagcca cgagcatgcc
     1561 gaccactgga ccccgcagca gctcgatcgc atcctcggcg gggcgagcgg cccggtgccg
     1621 atcatcgggc ccgaaggcgt cgcccgggcc gcgcacggct acgacgtgac cgtcgcccgg
     1681 gccggcgaga gcctcgaggc ttcggggttc acgctgcgct tcttcggcgg gcgccacgcg
     1741 gtcatccacc gctcgatccc ggtcgtcgac aacctcgccg ttctcgtcga cggcctgctc
     1801 ttccacccgg gagactcgtt caccgttccc gacgacacgc gggtcgacac gctggcggtg
     1861 ccggcgagcg cgccgtggtt gaaggcctcg gagttcatgg actacgtgct cgccgtgcgc
     1921 ccgaaacgct ccttcgccat gcacgagtgc atcaactccg aggcgggtaa tcggatggcc
     1981 ttcgaacgca tcaagacgac gacggaggct ggcggcggca ccttcctacc cctgcgggcg
     2041 ggcgacgaga ccgatctcta agagccggcc ccgcggtctc gaaagaacgg cgcccctgcc
     2101 ggacggaacc cggcaggggc gcttcgcgcc gacgcatcat gcggcccggc ggggccgggc
     2161 tcggcgctcg tacgccctgg ccctggcctt ccagccggcg aggagccggt cgagctcacg
     2221 atcgctcgtg cgggccgagt gctcggaaag ctcctgaccg aaccgttcac gttccgctac
     2281 cggcaaggct gcagaccagg ggaaggccgc ctgtaaccgc gagagcctcg ccgcgtgata
     2341 gagctgctcg gcccggcgat ccgtgaactt gcggggcgcc ctcgtttgtc gactcgacat
     2401 gccgctctcc cctttccctc cgatggcatg cgcgctatgc gtatcctcta tcacagacaa
     2461 ccatcgagac gccgggaata ttctcggcgc cggaaacagc cgttcgcggg gaccgcccgg
     2521 caggtggacg gagggcgtgg ggcgcttcag agcttgcgcg gcctcgacca ccagaactcg
     2581 agttggatgt tgtccgggtc acggaagacg agcgccgtgc ccgacccgga gggcgagtcg
     2641 gtgaccggtg aatgctcgac gccaagctcg gtgagcctcg cctcccacgc gtcgagatcc
     2701 tctcgcttcg cgacggcaaa accgacgtgg tccaaccccg gccgacgctc gtcgaaacgg
     2761 tcggcggcgc tctgacggtg ttggttcagc ccgatcacga gcccggtctc tcggtgcagc
     2821 aggacgacca tgatgcggtt gtcgagatct gagcgtgaga gctgcttcaa gcccagggtc
     2881 tcgacgtacc aggctgcgct cctcgccacg tcggagaccg agaggtcgag atgagagatc
     2941 gaacggaatt ctggcatgcc atctccttgt caagaccacg ctacgccggc cggtacggcc
     3001 ccgacgggtc tcggcgatcc gccgggcccg tcacacgtca ctcgccggcg agcccgccca
     3061 agaggttccc gaacgagtgc cccttgccga ggtaggtgtc gggatcgaac ggatcgctga
     3121 cgctcgccgg gtcgcggcgg gcgaccttgg ccgcgagctc ggtggccccc ttacggatgc
     3181 gctccgccag agtggcgact tggtcgccac tgccgcccca atcggcggag gcggcgaaga
     3241 ccgccgtgga gacggggtcg gcgtgcaggt aggcgaagag cggacgcatc gcgtagtcga
     3301 tcgcgaggct gtgccgcggc gtgccggccg tcgcggccaa gagcaccggg atgtcgagaa
     3361 gcgcctcttt gtcgagcaca tcgatgaacg acttgaagag gcccgaatag ctggtcgaga
     3421 agatcggggt cgcgaaaatc accgcgtcgg ccgagacgac cgcgttgatc atcgattcga
     3481 gcttgggcga ggcgaatccg gcgagcatgt tgtccgtcac gtcatgcgcg tagtctcgca
     3541 gctcgaagac gtcggccccc gcctcgatac cgtggtcctg cagctggtcg agcgtcgcct
     3601 cgacgagccg gtcgacgagc atcctcgtcg aggagggcgt cgcgaggcct gcgccgacgg
     3661 cggcgaggcg ccgggcgctc atcgcacggc ctccgccgcg ccacggcctg cctcggcact
     3721 ctcagcctcg ccggccgtca cggacgcttc gtatgggctc gccccggccg tgagattgtc
     3781 gccccggttc gcgttcggcc gcgactgacg cacggggccg tcgccgtact gcgcccgaac
     3841 gagagacgcg tgcgtcggcg catccggcac cgtggcaggc ctgccctggg cgagctcgcg
     3901 tcgcagcacg ggaacgacct cgccgcccaa gaggtcgatc tgttccagaa cggtcttcaa
     3961 cggcagcccg ccgtgatcca gcaggaagag ctggcgctgg tagtcgccga acaggtcgcg
     4021 catgccggcg taacggtcga tgacctgctg cggcgagccg acggtcaggg gggtcatctt
     4081 cgtgaagtct tccatgctcg gcccgtgacc gtacaccgga gcattgtcga aatacggccg
     4141 gaactcgtcg agggcgtcct gcgagttttt gcgcatgaac gcctgcccgc cgagcccgac
     4201 gatggcctgc tcgggtgtgc cgtggccata gtgggcgaag cgctgccggt agagctcgat
     4261 gagcctctgg tagtgctcgg ccggccagaa gatgttgttg gcaaagaagc cgtcgccgta
     4321 atatgccgcc tgctcggcga tctcgggggt gcgaatggaa ccatgccaca cgaagggcgg
     4381 cacgccgtcg aggggccgcg gtatcgatgt gaagccctgc agcggcgtgc ggaaccggcc
     4441 cgaccagtcc acgacgtcct cacgccacag ccgatgcagc agcgcgtagc tttcgaccgc
     4501 caactccagc ccctggcgaa tgtctttgcc gaaccacggg tagaccgggc cggtgttgcc
     4561 gcggcccagc atgaggtcgg cgcgcccgcc ggagacgtgc tggaggacgg cgaagtcctc
     4621 ggcgaccttg accgggtcgc tggttgtgat gagcgtcgtc
//
LOCUS       DASTNL010000116        10097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_84952, whole genome shotgun sequence.
ACCESSION   DASTNL010000116 DASTNL010000000
VERSION     DASTNL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84952"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFQ96_03650"
     CDS             <1..968
                     /locus_tag="VFQ96_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HEU0256914.1"
                     /translation="AVSGVRIGADPLGGASVHYWEAIRDRYGLNLDVVNPRIDPQWAF
                     MTLDWDGKIRMDPSSPSAMASVVARRYDFDILTGNDADSDRHGIVTPDSGLMNPNHFL
                     AVAVDYLYRHRPGWRADAAVGKTLVSSSMIDRVATALGRRLWEVPVGFKWFVPGLEDG
                     SVAFGGEESAGASFLRLDGTAWTTDKDGILLALLASEIRAVTGASPSEMYRGLTDRFG
                     APAYRRTDAKATPEEKARLRGLGAGQVTATTLGGDPITAILSTAPGNGAAIGGVKVAT
                     EQAWFAARPSGTEDVYKIYAESFRGPDHLAQVEAEARAIVNAALT"
     gene            complement(993..1937)
                     /locus_tag="VFQ96_03655"
     CDS             complement(993..1937)
                     /locus_tag="VFQ96_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HEU0256915.1"
                     /translation="MERPEAAISRRTMLLSVLAGSGALALAGCTAAARGPVGRGPATR
                     ATSQKTRASASPTARPTLRLPSRPGDVLGGSVTLTGTGLAAVTAVRFAAAEATIASTT
                     ATSVAVTIPAALDYQPATVPVTLVTPAGTFAAGGYTYAALTPVGRQMAYALKYWKHYN
                     TARYGSLNSVGGDCANFASQTLVARGWAMNDTWYNHDAAEHWTPAWGYVPAMDDYFLS
                     HGAALGLERLAEDQRDRVARGDLGVFDWENNGDRDHVEVVDRITRTDDGTIKVALASH
                     NLDYAYRDLDETITKQHPGATMHFWHFIDPTAVGGLAA"
     gene            2223..2678
                     /locus_tag="VFQ96_03660"
     CDS             2223..2678
                     /locus_tag="VFQ96_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0256916.1"
                     /translation="MALAKRTARVLIGSTIAGLAYGAWRAPAGRVAKAAPLLSKMRAA
                     LPFIPASDEKAVRLNGAVQTAAGVALITGVAPRLASTTLVLTLIPTTLAGHAFWKIKD
                     PAARRAQRMQFQKNAAIIGGLILSAEAPARRRRRRKRSAKVRGELRGRR"
     gene            complement(2675..3625)
                     /locus_tag="VFQ96_03665"
     CDS             complement(2675..3625)
                     /locus_tag="VFQ96_03665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0256917.1"
                     /translation="MSPRGADTRIQYDRYGGPDEMRLEPFTPAAPGEGEVRVRMRAAA
                     VNALDWKIRNGDMKFMTGRRFPRGLGHDFAGVVDAVGGGVDDYHTGDEVFGRASIKAA
                     GAFADRIVVPTSLLARKPAGVSFEQAAAMGTVGMTALQALITNGKLTAGQSVFINGCL
                     GGVGRVAAQIAAAHGIAVAGSCRADQREDAGRLGVSPVVGFDFDPEPFRGRFDLVFDT
                     AGVLPYSAARTLMKPGGRIIDINATPAKLVRSALPGPFRLQFTKTDSGDLRVIADAVQ
                     AGRLDLQIARTVPLDAAIHALTELETHNTPRGGKLVITAG"
     gene            complement(3622..4035)
                     /locus_tag="VFQ96_03670"
     CDS             complement(3622..4035)
                     /locus_tag="VFQ96_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0256918.1"
                     /translation="MLNADDRLAIVQTLSLNGHLVDDGDVDRFGETFTSDVVYELGAA
                     GVGTARGLEAVCAAAQGMAKAGAGPLAHHVTNIVITGGEGDTATARSKVLLVMRTGKL
                     ESAIQRDTLRRDGGTWRISHRVIAPLGDGAKEGER"
     gene            4148..4738
                     /locus_tag="VFQ96_03675"
     CDS             4148..4738
                     /locus_tag="VFQ96_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0256919.1"
                     /translation="MAATVSLVERKRRRARERIVQAADELFSAHGYDKVSVSDIAERA
                     EVGRTTFFRYFGDKPEVVFAKQQELLDAIAAARDRDAGAGPRTTVEAIEQLRPIVLDL
                     CSEVTSNPDGYRHHFALLADHAELRGRDALKTQQVADALNELLLHRGCEPATARLSAE
                     LSLACLQTPIKLANDPETLRADVNATFDRALALGRP"
     gene            complement(4824..5654)
                     /locus_tag="VFQ96_03680"
     CDS             complement(4824..5654)
                     /locus_tag="VFQ96_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0256920.1"
                     /translation="MRVTPGLRRVAYLVAALVIAAAFVAVYEFFVRTHAGQVVDERAF
                     LGASLWRSGLVAPLDRILTVLPLVSFGGGAILVLLIAAVRRNGKVLVAAIAAALAANA
                     TTQLLKYAILDRPNMGIGPQLANSMPSGHTTAAATTALAVFLVSSPGLRPLVATLGAA
                     FSTLIGAATLINQWHRPSDVIAALLVVAFWACAAGFALADSEEARAASPRRGPALPLL
                     VGAASCAIVAGYGFRVTLVSTATPDAHLLTAYAGGIASIAAVALGLAWVGIIAFRWVR
                     "
     gene            complement(5867..6778)
                     /locus_tag="VFQ96_03685"
     CDS             complement(5867..6778)
                     /locus_tag="VFQ96_03685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU0256921.1"
                     /translation="MGVDWSPESTSLVIGAENMRVLPGLPDASFTVVYLDPPFNTGRV
                     QARRTTRHVRREAALEAVAGGGPAPGLIVTGFQGRDYERIRGKLRRYDDRFDDYWAFL
                     EPRLAEAWRLLAPDGTLYLHLDYREAHYAKVLLDALFGRRSFLNEVIWAYDYGGKPRN
                     RWPAKHDTILVYVKDPASYFFDSAAIERIPYMAPGLVTAEKAARGKLPTDVWWHTIVS
                     PTGREKTGYPTQKPEALLRRMLQASSRQGDWVLDFFAGSGTTGAAAASIGRRFVLVDE
                     NPDALAVMRDRLQNVSGIRFEALADGR"
     gene            complement(6810..7859)
                     /locus_tag="VFQ96_03690"
     CDS             complement(6810..7859)
                     /locus_tag="VFQ96_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEU0256922.1"
                     /translation="MHGEYKVPGGKLVVVDLDVVGGLLANVHVAGDFFLEPDSALAVI
                     NDAVTGLPDTADASAIAAAVRGALPADAVLLGFSPEAVGVTVRRALTRATDWRDYDWT
                     LIHDGAVPPAVHLALDEVLAGEIGAGRRGPALRIWEWDESAVVIGSFQSVKNEVDPEG
                     ARKHGFQIVRRISGGGAMMMEKGAIITYSLYVPAALVQGMTFADSYAYLDEWVIEALR
                     SLGIDASYVPLNDISSPSGKIGGAAQKRFGNGAVLHHATMSYDMDGQKMTEVLRIGRE
                     KLSDKGTTSAAKRVDPLRRQTGLTRAQIIERMKETFTARYGATVGHVTADELAQAEAL
                     VASKFATKEWLYRVP"
     gene            complement(7907..8776)
                     /locus_tag="VFQ96_03695"
     CDS             complement(7907..8776)
                     /locus_tag="VFQ96_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0256923.1"
                     /translation="MDVQWFTERDGASIAFREWLVPNPRALVQIVHGVGEHSGRYGAL
                     AADLNAVGYAVYADDHRGHGLTGMRQWHGDARKLGRPGHGGVRAAEGSVRRFTELLQA
                     RNPGLPVVLLAHSWGSFIAQRLLDEDARDGRAAALPYAAVVLSGSAYRMPGWINAGDL
                     NAEFAGEGGTGSEWLSRDPGVARAFAVDPLTTSTPLRTLFGLIDALRILGRPRPGLPT
                     GLPLLLQVGGDDPVGGPRSVERLARAYRERGGLTDVTVLSYPGARHEVYNETNRAEVV
                     GDLLAWVAAHLPA"
     gene            9197..>10097
                     /locus_tag="VFQ96_03700"
     CDS             9197..>10097
                     /locus_tag="VFQ96_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0256924.1"
                     /translation="MNEHAMYWGIAALSAIVIVSYVFSIVSNKTRIPSVLMLLVLGIG
                     LRQAVTATGTTAPVGSQTLQFLGVLGLIVILLEAGLDLTLSRKKAPLIRRAVLSSLIL
                     LATTTAGIAVILYVLFGHSLMRSIVYAVPLSVISSTIVASSIHYLAKDKREFLTYESA
                     LSDIFGILLFNFLIAGGSGAGLIAFDLASLVVAVAASVVVSIVLVFLLAKVNINIKAF
                     LIFAVLLLIYAVGYASRLPTLLTVLIFGLIINNWRASSFRPFHRYLKPGDVEAAAETV
                     KSVTGEAAFLIRTMFFVLFGYTID"
BASE COUNT         1544 a         3532 c         3499 g         1522 t
ORIGIN      
        1 tcgccgtctc gggcgtccgg atcggcgccg acccgttggg cggggcgagc gtgcactact
       61 gggaggcgat ccgcgaccga tacgggctga acctcgacgt cgtgaacccg cgaatcgacc
      121 cgcaatgggc ctttatgacc ctcgactggg acggcaagat ccgcatggac ccttccagcc
      181 cctccgcgat ggcgtcggtg gtcgccaggc gctacgactt cgacatcctg accggcaacg
      241 acgccgacag cgaccggcat ggcatcgtca cgcccgactc ggggctgatg aacccgaacc
      301 atttcttggc ggtcgccgtc gactacctct atcggcaccg gccaggctgg cgggcggacg
      361 ccgcggtcgg caagaccctc gtctcgtcct cgatgatcga ccgcgtcgcc acggcgctcg
      421 ggcgccggct gtgggaggtg ccggtcggct tcaaatggtt cgtgccgggt ctcgaagacg
      481 ggtcggtcgc cttcggcggc gaggagagcg cgggggcgag cttcctgcgt ctcgacggca
      541 cggcgtggac gacggacaaa gacggcatcc tcttggcgct cctcgcctcc gagatccggg
      601 ccgtgacggg cgcgagcccg agcgagatgt accgggggct caccgaccgg ttcggcgcgc
      661 ccgcctaccg gcgaaccgac gccaaggcga cgccggagga aaaggcgcgg ctgcgggggc
      721 tgggggcggg gcaggtgacg gcgacgacgc tcggcggcga cccgatcacg gcgatcctca
      781 gcaccgcgcc cggcaacggg gcggcgatcg gcggggtgaa ggtggcgacc gagcaagcct
      841 ggttcgcggc gcggccgagc ggcaccgaag acgtgtacaa gatctacgcg gagtcgttcc
      901 gcggccccga ccacctcgcc caggtagagg ccgaggcacg cgcgatcgtg aacgccgcgc
      961 tcacctgacc ggccgccacg ccgcgcggcg aatcaggcgg cgagcccgcc gacggcggtc
     1021 gggtcgatga agtgccagaa atgcatcgtc gcgccggggt gctgcttcgt gatcgtctcg
     1081 tcgaggtcgc ggtaggcgta gtcgagattg tggctcgcga gcgcgacttt gatcgtgccg
     1141 tcgtcggtgc gcgtgatgcg gtcgacgacc tctacgtgat cccggtcgcc gttgttctcc
     1201 cagtcgaaga cgccgaggtc tccccgggcc acccggtccc gttggtcttc ggccagccgt
     1261 tcgaggccga gagcggcacc gtgagagaga aagtagtcgt ccatcgccgg cacgtacccc
     1321 caggccggag tccaatgctc tgccgcgtca tggttgtacc aggtgtcgtt catcgcccag
     1381 ccgcgggcga cgagggtctg gctggcgaag ttggcgcagt cgccgcccac ggagttgagg
     1441 ctgccgtatc gggcggtgtt gtaatgcttc caatatttca acgcgtaggc catctggcgc
     1501 ccgaccggcg tcaacgccgc ataggtgtag ccgccggccg cgaaggtacc ggccggcgtc
     1561 acaagcgtga ccggcacagt ggccggctga tagtcgagcg cggcgggaat cgtcacggcc
     1621 acggaggtcg ccgtcgtcga tgcgatcgtg gcctcggcgg ccgcgaaccg cacggccgtc
     1681 acggcggcga gcccggtgcc ggtgagggtc acgctgccgc ccagcacgtc gcctggccgg
     1741 ctcggcaggc gcagcgtggg cctcgccgta ggcgaggcgg aagcccgtgt cttctgcgag
     1801 gtcgcccgcg tggccgggcc gcggcccacc ggcccacggg cggcggcggt gcagccggcc
     1861 agggcaagcg ccccggagcc ggccagcacg gaaagaagca tggtgcggcg gcttatcgcc
     1921 gcctccggtc gctccatcgg cccctcctta cgccctcgcc cgcgcttgcg accttccggg
     1981 gttcgggccc agaatcccga atcggctgcg gccgatcctg ctcgccgccc gcacccgcgc
     2041 gacccgcccc ggtacggcac ccgggccgcc ttcgcgcccc gccctgcaac gacgagacgt
     2101 gccggccacc cggcaacgga gcgtggtgcg tcggcggagc cgagtctaac cggctcgcct
     2161 ggccgcccgg atgcgtctgc cggcgatggt gccgcggggc ggaccggtcg tagtatcgag
     2221 gcgtggcttt ggcgaagagg acggcgcggg tgctgatcgg ctccacgatc gccggcttgg
     2281 catatggggc gtggcgggca ccggccggtc gcgtggcgaa ggcggcgccg ctcttgtcca
     2341 agatgcgtgc cgccctgccg ttcatccccg cctccgacga gaaggcggtg cgcctcaacg
     2401 gcgccgtgca gacggcggcc ggtgtggcct tgatcaccgg ggtcgcgccc aggcttgcct
     2461 cgacgacact cgtcttgacc ctcatcccga cgaccctggc cggccacgcg ttctggaaga
     2521 tcaaagatcc cgcggcacgc cgggcacagc ggatgcagtt tcagaagaat gcggcgatca
     2581 tcggcgggct cattctttcg gccgaggccc cggcacgccg ccggcgacgg cgcaagcggt
     2641 ccgcgaaggt gcgcggggag ttgcgcgggc gccgctaacc ggccgtgatc acgagtttgc
     2701 cgcctctcgg ggtgttgtgg gtctccagtt cggtcagtgc gtggatggcc gcgtcaagcg
     2761 gcaccgtgcg ggcgatctga aggtctagtc gacctgcttg caccgcgtcg gcgatcaccc
     2821 ggaggtcgcc tgagtcggtc ttggtgaact gcagccggaa gggtccgggg agggcgctgc
     2881 gcacaagttt ggccggggtc gcgttgatgt cgatgatccg cccgccgggc ttcatcagcg
     2941 ttcgtgcggc gctgtaaggg agcacacccg ccgtgtcgaa gacgaggtcg aaccggcccc
     3001 gaaagggttc cgggtcgaag tcgaaaccga cgaccggcga aaccccgagg cgccctgcat
     3061 cctcacgctg gtcggcccgg cagctgccgg caacggcgat cccgtgggca gcggcgatct
     3121 gggcggcaac gcgcccgaca cccccgagac agccgttgat gaagacgctc tggccggcgg
     3181 tgagcttgcc gttcgtgata agtgcctgga gcgcggtcat gccgacggta cccatggccg
     3241 cagcctgctc gaagctcacc ccggccggct tcctcgcgag aagcgaggtg ggcacgacga
     3301 tccggtcggc gaacgcgccg gctgccttga tgctggccct gccgaacacc tcgtcccccg
     3361 tgtggtagtc gtcgacgccg ccaccgaccg cgtcgacgac gccggcgaag tcgtgcccca
     3421 acccgcgcgg aaagcgccgg ccggtcatga atttcatgtc gccgttgcga atcttccaat
     3481 ccagggcgtt cacggcggcg gcccgcatcc gcacccgcac ctcgccctcg cccggggcgg
     3541 ccggggtgaa aggttcgagc ctcatctcgt cggggccgcc gtagcggtcg tattggatgc
     3601 gggtgtctgc gccccggggg ctcatcgctc cccctcctta gcgccgtcac ccaagggggc
     3661 gatgacccgg tgactgatcc gccaggttcc accgtcgcgg cgtagcgtgt cacgttggat
     3721 ggcactctcg agctttcccg tgcgcatcac caagagaacc ttggaccggg cggtggcggt
     3781 gtcaccctcg ccaccggtta tgacgatatt cgtcacgtgg tgggcgagcg gcccggcccc
     3841 ggccttagcc atcccctggg cggcggcgca caccgcctcc aggccgcggg ccgttccgac
     3901 cccggcggcg ccgagctcgt agacgacgtc tgacgtgaag gtctccccga agcgatcgac
     3961 gtcgccgtcg tcgacgaggt ggccgttcaa cgacagggtc tgcacgatcg cgaggcgatc
     4021 gtcggcattc agcatgagtg tttcctttcc gaaccggcca tacggttcgt ggtctcgttt
     4081 ggtactcagt accatagcat actaagagcc tatcggaact gtgatcggtt cagggtatga
     4141 tgccctcatg gcagcgaccg tctccctcgt cgaacggaag cgtcggcgcg cccgcgagcg
     4201 catcgttcag gccgccgacg agctgttctc agcgcacggc tacgacaagg tctcggtgag
     4261 tgatatcgcc gaacgtgccg aggtcggccg caccacgttc tttcgctact tcggcgacaa
     4321 gccggaggtc gtcttcgcca agcagcaaga gctgctcgac gccattgccg ccgcccggga
     4381 ccgcgacgct ggcgccggcc cccgcaccac ggtcgaagcg atcgagcagt tgcgcccgat
     4441 cgtcctcgac ctctgctccg aggtcacctc gaaccccgac gggtaccgcc accacttcgc
     4501 cctgctcgcc gaccacgcag agctgcgcgg ccgcgacgcc ctcaaaacac aacaggtcgc
     4561 cgacgccctc aacgagctcc tgctgcaccg gggctgcgag ccggccaccg cacgcctttc
     4621 ggccgaactc tccctcgcct gcctgcagac ccccatcaag ctcgccaacg acccggagac
     4681 actccgggcc gacgtgaacg ctacgttcga ccgggcactc gcgctcggcc gcccgtgagc
     4741 acctgcccga ccgccaaaga cgagacgcac cgactcttca cgcgcagggc atggcggagc
     4801 gagacccggc gcgtgaagga gcctcagcgc acccagcgga acgcgatgat gccgacccac
     4861 gcgaggccaa gggcgaccgc ggcgatcgaa gcgatgccgc ccgcgtaggc ggtgagaaga
     4921 tgcgcgtcgg gcgttgccgt gctgacgagc gtgacgcgga acccgtaccc ggcgacgatc
     4981 gcgcacgagg ccgcgccgac gagcagaggc agggcgggcc cccgccgcgg cgacgccgcc
     5041 cgcgcctcct cgctgtcggc aagcgcgaag cctgccgcgc aggcccagaa cgcgaccacg
     5101 aggagggccg cgatgacgtc gctcgggcga tgccactggt tgatcagcgt cgccgcgccg
     5161 atgagcgtcg agaacgccgc ccccagcgtg gcgacgaggg ggcgcagacc gggcgagctg
     5221 acgaggaaca ccgcgagcgc cgtcgtcgcg gccgccgtcg tgtggccgga gggcatcgag
     5281 ttcgccagct gcggcccgat tcccatgttc ggccggtcga ggatcgcgta cttcaagagc
     5341 tgcgtggtcg cgttcgccgc cagcgccgcg gcgatggccg cgaccagcac tttgccgttg
     5401 cggcgtaccg cggcgatgag aagcacgagg atggcgccgc cgccgaagga gacgagaggg
     5461 agcaccgtga gaatgcgatc cagcggcgcg acgaggccgg aacgccatag gctggcaccg
     5521 agaaaggccc gctcgtcgac gacctgcccg gcgtgcgtgc gcacgaagaa ctcgtagacg
     5581 gcgacgaagg ccgcggcgat gacgagcgcc gccacgaggt atgccaccct tctcaacccg
     5641 ggcgtcacac gcataggaaa accctctcac ggctgaccgt acgcagcctt ggagcgccgc
     5701 gagacgcggc ccgtgccagg tgcggcacac tcacctcggg aaggccgccg gcgggcgacg
     5761 gcgggcgacg gcgggcgacg cgacaaccag cgcctatggc tagcccatac cctgcccgga
     5821 ggccttcgcc gacctcggcc ctccacgacc cgccagggaa gtacggctaa cggccgtcag
     5881 ccagagcctc gaaccggatg ccggagacgt tttggagccg gtcgcgcatg accgcgagcg
     5941 catccgggtt ctcgtcgacc aacacgaaac gccggccgat gctcgccgca gccgccccgg
     6001 tggtgccgct gccggcgaag aagtcgagca cccaatcccc ttgcctgctc gaggcctgca
     6061 gcatgcgacg cagcagggcc tccggcttct gggtggggta tccggtcttc tcgcggccgg
     6121 tgggcgagac gatcgtgtgc caccacacgt cggtgggcag cttgccgcgg gccgccttct
     6181 cggcggtcac gaggccgggt gccatgtagg ggatccgttc gatcgccgcg ctgtcgaaga
     6241 agtacgaggc cggatctttc acgtagacga ggatcgtgtc atgcttcgcc ggccagcgat
     6301 tgcgcggctt gccgccgtag tcgtacgccc agatgacctc gttgagaaag ctgcgccggc
     6361 cgaagagcgc atcgagcagc accttcgcgt agtgcgcctc ccggtagtcg aggtgcagat
     6421 acagggtgcc gtccggggcg agaagccgcc aggcctcggc gagacgcggt tcgaggaaag
     6481 cccagtagtc gtcgaaccga tcgtcgtacc ggcgcagctt accgcggatg cgttcgtagt
     6541 cacggccttg gaagccggtg acgatgaggc ccggcgcggg cccgccgccc gccaccgctt
     6601 cgagcgccgc ttcgcgccgc acgtgccggg tggtgcgcct ggcctgcacc cggcccgtgt
     6661 tgaagggcgg gtcgaggtag acgacggtga aggaggcgtc gggcaggccg ggcaggaccc
     6721 gcatgttctc ggcgccgatg acgagactgg tggattccgg tgaccagtcg acgcccatgc
     6781 ctcccattct cgcagggggt gcatccgcct cacggcacgc ggtagagcca ctccttggtt
     6841 gcgaacttcg aggcgacgag ggcctcggcc tgtgcgagct cgtcggcggt cacatgaccg
     6901 acggtggcgc cgtagcgggc cgtgaacgtc tctttcatcc gctcgatgat ctgggcacgc
     6961 gtcagcccgg tctggcggcg cagcgggtcg acgcgtttcg cggcggaggt ggtgcccttg
     7021 tcgctgagct tctcgcggcc gatgcgcaag acctcggtca tcttctggcc gtccatgtcg
     7081 tagctcatcg tcgcgtggtg cagcaccgca ccgttgccga agcgcttctg agcggcgccg
     7141 ccgatcttgc cgctcgggct ggaaatgtcg ttgagcggca cgtagctcgc gtcgatgccc
     7201 agggaacgca acgcctcgat cacccactcg tcgaggtagg cgtagctgtc ggcgaaggtc
     7261 atgccctgca cgagggcggc cggcacgtag agggaatagg tgatgatggc gcccttctcc
     7321 atcatcatgg ctccgccgcc cgagatgcgg cgcacgatct ggaagccgtg cttgcgcgcg
     7381 ccttccggat cgacctcgtt cttcacggac tgaaagctgc cgatgacgac cgcagactcg
     7441 tcccattccc agatgcgcaa cgccggcccg cgccggcccg cgccgatctc gccggccagc
     7501 acctcgtcga gggcgaggtg cacggcgggc ggcacggcgc cgtcgtggat caaggtccag
     7561 tcgtagtctc gccagtcggt ggcccgggtc agcgcccgcc gcacggtcac gccgaccgcc
     7621 tcgggcgaga agcccagcag aacggcgtcg gccggcagcg cgccgcgcac ggcggctgcg
     7681 atcgccgacg cgtccgccgt gtcaggcagg ccggtcaccg cgtcgttgat caccgcgagg
     7741 gcggagtcgg gctcgagaaa gaagtcgccg gcgacgtgaa cgttcgcgag caagcctccg
     7801 acgacgtcga ggtcgacgac gacgagcttg ccgcccggca ccttgtactc accgtgcatg
     7861 cacccagcct attccgggcc gcgtacaact atccgggcgc ccgtgctcac gcaggcaggt
     7921 gcgccgccac ccaggcgaga aggtcaccga ccacctcggc gcgattcgtc tcgttgtaga
     7981 cctcgtggcg ggcacccggg taagagagca cggtcacgtc ggtcagcccg ccacgttccc
     8041 ggtaggcgcg ggcgaggcgt tcgacgctgc gcgggccgcc gaccgggtcg tcgccgccca
     8101 cttgcagaag gagcggcagg ccggtcggca gccccggccg cggcctgccg agaatgcgca
     8161 gcgcgtcgat gagcccgaag agggtgcgca gcggcgtcga ggtggtgaga gggtcgacgg
     8221 cgaaggcgcg ggcgacgccc gggtcacggc tcagccactc ggagccggtg cctccctcgc
     8281 cagcaaactc ggcgttcagg tcacccgcgt tgatccagcc gggcatgcgg taggcgctgc
     8341 cggagagcac gacggcggcg taaggcagcg cggcggcacg gccatcccgc gcgtcttcgt
     8401 cgaggaggcg ctgcgcgatg aaggagcccc agctgtgcgc caggaggacg accggcaggc
     8461 cagggttccg tgcctggaga agctcggtga aacggcgcac ggagccctcg gcggcacgca
     8521 caccgccgtg gcccggcctg cccagcttgc gggcgtcgcc gtgccactga cgcattccgg
     8581 tgagtccgtg gccgcgatgg tcgtcggcgt agaccgcgta acccaccgcg ttcaggtcag
     8641 cggccagggc gccgtagcgg ccggagtgct cgcccacgcc gtgcacgatc tgaacgagcg
     8701 cgcgagggtt cggcaccagc cactcgcgaa aggcgatcga agccccgtca cgttcggtga
     8761 accattgcac atccacacgc cgatcctgcc gcaccgccac ctggcgcgcg aggacgggcg
     8821 cgcgaaatca atagaccggc gaaataatcg ggtccggcgg gtgtggctca cggcgtcggg
     8881 cgagggatga tgcggcgccc ggccgccggt gagccacgac ccggcagcct ccgctacttt
     8941 gccgcggcgc atcacggcga ggtttgccag actgtgaact tgtgccttct tcgaccgcca
     9001 cgctcgggcc agatcccacc cgcttgtccg ctccccgcct cgcccgcggg cgtggcaggc
     9061 ccgcgacgcc cgcgggcgca gtggacgcgc tcgacacggc accaccatct gacgccccgt
     9121 cgccgccagg ttccgcctgg ccaccggctc gcccgcaggc tcaagccggc gccgcggccc
     9181 tgacggaggc gatgcgatga acgaacacgc catgtattgg ggcatcgcgg cgctgagcgc
     9241 gatcgtgatc gtgtcttacg tcttctccat cgtcagcaac aagacccgga tcccgtcggt
     9301 gctgatgctg ctggttctcg gcatcggcct ccgccaagcg gtgacggcca ccggcacgac
     9361 ggcgccggtc ggctcccaga ccctccagtt cctgggcgtc ttagggctca tcgtgattct
     9421 cttggaggcg gggctcgacc tcaccctgtc gaggaagaag gcgcccctca tacgccgagc
     9481 ggtgctctcc tcactgatcc tcttggcaac gacgacggcg ggcatcgccg tgatcctcta
     9541 cgtcttgttc ggccactcgc tgatgcgcag catcgtctat gccgtgccgc tgtcggtgat
     9601 cagcagcacg atcgtcgcct ccagcatcca ctacctcgcg aaagacaagc gagaattcct
     9661 cacctacgag tcggcgctct ccgacatctt cggcattctg ctcttcaatt tcctcatcgc
     9721 gggaggctcc ggcgccggcc tgatcgcctt cgatctggcg agcctcgtcg tcgccgtcgc
     9781 cgcctcggtg gtcgtctcca tcgtgctggt gttcctcttg gcgaaggtga acatcaacat
     9841 caaggcgttc ctgatcttcg ccgtgctgct gttgatctac gcggtcggct acgcctcgcg
     9901 gctgccgacc ctcctgaccg tgctgatctt cgggctcatc atcaacaatt ggcgggcaag
     9961 cagcttccgc ccgttccacc gctacttgaa gccgggcgac gtggaggcgg cggccgagac
    10021 cgtgaaatcg gtcaccggcg aggccgcctt cctcatccgc accatgttct tcgttctctt
    10081 cggctacacg atcgacg
//
LOCUS       DASTNL010000117         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1009289, whole genome shotgun sequence.
ACCESSION   DASTNL010000117 DASTNL010000000
VERSION     DASTNL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009289"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFQ96_03705"
     CDS             complement(<1..911)
                     /locus_tag="VFQ96_03705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU0256925.1"
                     /translation="MSRSDDTPSIERWDHPVSIRMVVTDIDHTLITDDHRLTERTVRS
                     VRAARQRGVEVVLASSRPPLGMTGYLGELGLIDPEPFIALQGGLIATLADPGTAGDVA
                     AASGAGVAGAGPDLRVLHSSPIPMRLARRVVAVGEAMGVSVNWYSGTRWFVSRYDDRA
                     RREAGVVGTEPVETSLASVRHDPEKILFIARDAEGSERLRELPLPAGVEATFSNNGYL
                     EVTAAGVDKGSALRIVANAAGVPTGQVAAIGDGENDLAMFRAAGVGIAPANASAQVRQ
                     AAGFVTASNNADGVAVALEALLGPLVPR"
     gene            complement(908..1840)
                     /locus_tag="VFQ96_03710"
     CDS             complement(908..1840)
                     /locus_tag="VFQ96_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU0256926.1"
                     /translation="MTAIRLDGKVTAAALKAELAERVRVLREQGVTPGLGTLLVGDDP
                     ASRSYVGGKHRDCAEVGIESVRADLPADASPDDVRRAIDDLNANPAVTGFIVQLPLPA
                     GIDEGEMLRRVDPAKDADGLHPANLGALVLGVSGTVTTPLPCTPAGILELLRRYDVPL
                     AGRHVVVIGRGITVGRPLGLLLTRKGVDATVTLTHSRTVDLAAEVRRADIVVAAVGRP
                     GLVRPDWVRPGAAVVDVGVSRVGTAPSGKAKLAGDVDPAVAEVAGHLSPVPGGVGLMT
                     RAMLLENVVTMAERALGGVSAAAESAATPAGAGR"
     gene            complement(1860..3188)
                     /gene="glyA"
                     /locus_tag="VFQ96_03715"
     CDS             complement(1860..3188)
                     /gene="glyA"
                     /locus_tag="VFQ96_03715"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0256927.1"
                     /translation="MTDSTFTRPLSEVDPEIAALLESELGRQRDYLEMIASENFVPRA
                     VLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVVEQLAIDRAKALFHAEYANVQPHAGAT
                     ANAAVLHALAKPGDTILGMELAHGGHLTHGMRINFSGKFYNAESYQVDPKTFLVDMDA
                     VRDAALQHRPSVIIAGWSAYPRQLDFAAFRAIADEVGAKLWVDMAHFAGLVATGLHPS
                     PVPYADVVSSTVHKTIGGPRSGFLLSRDTSLAKKLNSAVFPGQQGGPLMHIVAAKAVA
                     FKLAASADFQDRQERTVRGAKILAQRLTAADSRAAGVDVLTGGTDVHLVLADLRESAL
                     DGKQAEDRLHEVGITVNRNAVPFDPRPPMVTSGLRIGTPALATRGFDDAAFTEVADVI
                     AEALKPSGDLTALRRRVTALAAQFPLYDGLNAFGRPSSELADAPSAALVH"
BASE COUNT          483 a         1127 c         1152 g          459 t
ORIGIN      
        1 cggggcacga gcggcccgag caacgcctcc agggcaacgg cgacgccgtc ggcgttgttc
       61 gaagccgtaa cgaaacccgc ggcctgccgc acctgggcag aggcgttggc cggcgcgata
      121 ccgacaccgg ccgcccggaa cattgccagg tcgttctcac cgtcgccgat cgccgcgacc
      181 tggcccgtcg ggaccccggc cgcattcgcc acgatccgca gagcggagcc cttgtccact
      241 cccgcggcgg tcacctcgag gtacccgttg ttggagaagg tcgcctccac tccggccggc
      301 aaggggagct ctcggagccg ttcggagccc tcggcgtcac gggcgatgaa aaggatcttc
      361 tccgggtcgt gccgtaccga ggcaagactg gtctcgaccg gctcggtgcc gacgacgccg
      421 gcctcccgcc gcgcccggtc gtcgtagcgg gagacgaacc aacgggtgcc cgaataccag
      481 ttgacgctga cgcccattgc ctccccgacc gccacgaccc gtctggcgag ccgcatcggg
      541 atagggctcg agtgcagcac ccgaaggtca gggccggcgc ccgccacacc ggccccgctt
      601 gcggcggcga cgtcacccgc cgtgccgggg tcagccagcg tcgcgatcaa gccgccctgc
      661 agggcgatga aaggctctgg atcgatcaag ccaagctcac ccaggtaacc cgtcatgccc
      721 agcggcgggc gggaggaggc caggacgacc tctacgcctc tctgccgtgc ggcgcgcaca
      781 ctgcgcacgg tgcgctcggt caggcgatgg tcatcggtga tcagggtgtg gtcgatgtcg
      841 gtgacgacca tccggatcga gaccgggtgg tcccagcgtt cgatcgaggg cgtgtcgtcc
      901 gaccggctca ccggccggca ccggcaggcg tggcagcact ctccgcagcc gccgatacac
      961 ctcccagcgc ccgctcggcc atggtcacga cgttctccaa cagcatcgcc cgggtcatga
     1021 ggcccacacc gccgggcaca ggagagagat ggccggcgac ctccgcgacc gcgggatcga
     1081 cgtccccggc gagcttagcc ttgccgctcg gcgccgtgcc gacacggctc accccgacat
     1141 cgaccacggc ggcgcccggt cgcacccagt cgggccggac gaggcccggg cggcccacgg
     1201 cggccacgac gatatcggcg cgacgcacct ccgccgccag atcgaccgtc cgtgaatggg
     1261 tgagcgtcac ggtcgcgtcc acgcccttgc gggtcaggag caggccgaga ggccgcccga
     1321 ccgtgatacc gcgcccgatc acgacgacat gccgaccggc caagggaacg tcgtagcggc
     1381 gcagcagctc gaggatcccg gccggggtgc agggcagcgg cgtggtcacc gtgccggaga
     1441 ctcccagcac gagcgcgccg agattggcgg gatggagccc gtcggcgtct ttggcgggat
     1501 cgacgcggcg cagcatctcc ccctcgtcga tgccggccgg cagtgggagc tgcacgatga
     1561 acccggtgac cgccgggttg gcgttcaggt cgtcgatcgc ccggcgcacg tcgtccggcg
     1621 acgcgtcggc cggcaggtca gcccggaccg actcgatgcc cacctcggcg cagtctcgat
     1681 gtttgccgcc gacatacgac cgcgaggccg gatcgtcccc gaccagcagg gtgccgagtc
     1741 cgggcgtgac gccctgctct cgcagcacgc gtacgcgctc tgccagctcc gccttcaatg
     1801 ccgccgcggt gaccttgccg tccaaacgga tcgccgtcat gggatctgcc gccctccgct
     1861 cagtggacga gcgcggcgga cggggcgtcg gccagctcgg aggaggggcg cccgaacgcg
     1921 ttcagcccgt cgtagagggg gaactgtgcg gcgagcgcgg tgacccggcg acgcaacgcc
     1981 gtgaggtcgc ccgacggctt gagggcctcg gcgatgacgt cggcgacctc tgtgaacgcg
     2041 gcatcgtcga acccgcgggt cgccaaggcc ggcgtgccga tgcgcaggcc ggaggtgacc
     2101 atcggaggcc gtgggtcgaa cgggaccgca ttccggttca ccgtgattcc cacctcgtgc
     2161 agacggtcct cggcctgctt gccgtccagg gcgctctcgc gcaggtccgc caggaccagg
     2221 tggacatcgg tgccgcccgt gagcacgtcg acgcctgcgg cacgagagtc ggccgccgtc
     2281 aggcgttgcg ccaggatctt cgcgccccgc accgtgcgct cttggcggtc ctggaagtcg
     2341 gcgctggcgg ccagcttgaa ggcgaccgcc ttcgccgcga cgatatgcat gagcgggccg
     2401 ccctgctggc cggggaagac ggcggagttg agcttcttgg cgaggctcgt gtcccggctg
     2461 aggaggaacc cgctgcgggg gccgccgatc gtcttgtgca cggtggagga gacgacgtcc
     2521 gcgtagggga cgggcgaagg gtgcaggccg gtcgcgacca ggccggcgaa atgcgccatg
     2581 tcgacccaca gcttcgcgcc gacctcgtcg gcgatcgccc ggaaggcggc gaagtcgagc
     2641 tggcgcgggt aggcggacca gcctgcgatg atcaccgacg ggcggtgctg cagggccgcg
     2701 tcgcgcacgg cgtccatgtc gacgaggaag gtcttcgggt cgacctgata cgactcggcg
     2761 ttgtagaact tgccggagaa gttgatgcgc atgccgtggg tgagatgccc gccgtgcgcc
     2821 agctccatgc cgaggatcgt gtcgcccggc ttcgccagcg cgtgcagcac ggccgcgttc
     2881 gcggtggcgc ccgcatgcgg ctggacgttc gcgtactcgg cgtggaagag cgccttggcc
     2941 cggtcgatgg ccagctgctc gacgacgtcg acatactcgc atccgccgta gtagcgccgg
     3001 cccggatagc cttcggcgta tttgttggtc agcacggacc cctgcgcctc gagcacggcg
     3061 cggggcacga agttctcgct cgcgatcatc tcgagatagt cgcgctgtct gcccagctcg
     3121 ctttcgagca gagcggcgat ctcgggatcg acctcggaga gcggtcgggt gaacgtggaa
     3181 tcagtcacgg ttgtcctcga tggggagtcg gctcaggtca c
//
LOCUS       DASTNL010000118         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1382301, whole genome shotgun sequence.
ACCESSION   DASTNL010000118 DASTNL010000000
VERSION     DASTNL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382301"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="VFQ96_03720"
     CDS             <1..1760
                     /locus_tag="VFQ96_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEU0256928.1"
                     /translation="FSDPALAERLNRRFVAVKVDREEHPDVDATYLAAAAGFTRQLGW
                     PLTVFLTPEGQAFYAGTYFPPTPSRGLPALSQVLDAVWHAWKGDRKQVSETAARLAAN
                     LGQLAGRAAPSGDALRRADVGALVDAAAEQALGREDEEHGGFGAAPKFPLAPALDFLI
                     EAGSTPDAVGPGDSAAGVSGRAAALRALRAMAGSDLRDPVEGGFFRYATMADWSVPHY
                     ERMLYDNAQLLSLYTHAVAGARNGDAVDDFDSAASGIAGFLIDVLGREGGGFGSAQDS
                     ESIVHGERSEGGYYALDAAGRSRETPPPVDGKVLAGWNGLAIAALADAGVTFARPEWV
                     GAARAAADRILATHRTEEGRLARASLDGVVSRAPATLEDYGLFSVGLLRLALAGGGAR
                     YAEAARRLVDTVLSGPGHEDTAKRPFTPPEAADPLLAAQGVEVLETEGGSTLPGGEVA
                     CAWAAHLLGQMTAEERYDLVAGRVVSARLESAAAEPLAAGDVLALAARRLSRRQIVVV
                     LPDHDVPGGLSAAIVAAALRLTGVHAVVSESESRALAAAGFTLFESRDCREGVPTVYV
                     CHDFVCELPITDPSLLGVS"
     gene            complement(1869..2492)
                     /locus_tag="VFQ96_03725"
     CDS             complement(1869..2492)
                     /locus_tag="VFQ96_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEU0256929.1"
                     /translation="MAVGLAAIGTAAIKGSEPRASAATSMSMNHKPVTLDPGEGVAPK
                     TPSPPPGQTHAPNASPRPKAGRLVVPSVGLSVPLGTMRETDGEITPPGFTSAYQIDNL
                     GVTPSNGRDGTVFVAMHSLRGGAIGPGNYLTDVAKGVAKVAIGALVEVDGVDYRITGW
                     ARVKKADLPYLPFVWNDTPNRLVMITCLELPSGAPSVDNMVLTAERD"
     gene            complement(2700..3104)
                     /locus_tag="VFQ96_03730"
     CDS             complement(2700..3104)
                     /locus_tag="VFQ96_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256930.1"
                     /translation="MPQTTTPPMPPCGPSTASSTPGTAQDPAGSDANGSTGASEPGEA
                     ADTTTSAATPAGQAAQAPSDPNNTNNIAVGVRVFSQAVVGPRVNTGGVLAPSSTTEPD
                     GLLLAGAGLLTLGVAVSAALVWAPRRESAQAR"
     gene            3109..>3386
                     /locus_tag="VFQ96_03735"
     CDS             3109..>3386
                     /locus_tag="VFQ96_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256931.1"
                     /translation="MLDVLGAAGALGQAGALGVVGADGVEAPTGAAGLAGAAGLAGAA
                     GLAGGATCIGCAACATPMPTSERGRAIAAARAYLATDFMLKTSMIFDQL"
BASE COUNT          426 a         1148 c         1268 g          544 t
ORIGIN      
        1 gcttcagcga cccggcgctc gccgaacgtc tgaacaggcg tttcgtcgcc gtcaaggtcg
       61 accgcgaaga gcatcccgac gtggatgcga cgtacctcgc cgcggctgcc gggttcacga
      121 gacagctggg ttggccgctg accgtgttcc tgacccccga gggccaggcg ttctatgcgg
      181 gaacctactt tccacccacg ccctcccgcg ggctccccgc cctcagtcag gtgctcgacg
      241 cggtgtggca cgcctggaag ggggatcgca agcaggtctc ggagacggcg gcccggctcg
      301 ccgcgaacct gggtcagctc gccggccgcg cggcaccctc cggcgacgcc ctccggcgcg
      361 ctgacgtcgg cgccttggtc gacgccgcgg cggagcaggc gttgggccgt gaagacgaag
      421 agcatggcgg gttcggtgcc gccccgaagt ttccgctcgc gccggccctc gacttcctga
      481 tcgaggccgg ctccaccccg gatgcggtgg gtcccggtga cagtgcggcc ggcgtctcgg
      541 gccgcgctgc cgcgttgcgg gcgttgcggg cgatggccgg ctccgacctg cgggatcccg
      601 tggaaggcgg tttcttccgg tacgcgacca tggccgattg gagcgtgccg cattacgagc
      661 ggatgctcta cgacaacgct caactgctct ccctgtacac gcacgccgtg gcgggcgcga
      721 gaaacgggga cgccgtcgac gacttcgact ccgcggcgag cgggatcgcg ggcttcctta
      781 tcgacgtgtt gggccgcgag ggcggcgggt tcggctccgc acaagattcc gagagcatcg
      841 tgcacggcga gcgcagcgag ggcggctatt acgcgctcga cgccgccggg cgaagccgcg
      901 agacgccccc gcccgtcgac ggcaaggttc tcgccggttg gaacggcctg gccatcgccg
      961 cactggccga tgcgggcgtc accttcgcaa gacccgagtg ggtcggtgcc gcccgcgccg
     1021 ccgccgatcg gattcttgcc acgcatcgca cggaggaggg ccggctcgcc cgcgcgtccc
     1081 tcgacggcgt cgtctcccgg gcacccgcga cgctcgaaga ctacggcctc ttctcggtcg
     1141 gcctcctgcg tctcgcactc gcgggcgggg gagcccggta cgccgaggcc gcgcgccggc
     1201 tcgtcgacac agtgctgtct gggcccggcc acgaggacac ggcgaagcgc ccgttcacgc
     1261 cgcccgaggc tgccgacccg ttgctcgccg cgcaaggcgt cgaggtgctg gagacggagg
     1321 gcggcagcac gctgccgggc ggtgaggtcg cctgcgcttg ggccgcgcac ctccttggcc
     1381 agatgacggc cgaggaacgc tacgacctcg tcgcaggccg ggtcgtctcg gcgcggctgg
     1441 agtcggcggc ggccgagccg ctggccgccg gtgacgtgtt ggccctcgcc gcccgccgcc
     1501 tctcccggcg ccagatcgtc gtcgtgctgc cggaccacga cgtgccgggc gggctttcgg
     1561 ccgcgatcgt ggccgcggcg ctgcgcctga ccggcgtgca cgcggtcgtc tcggaaagcg
     1621 agtcgcgggc gctcgcggcg gcggggttca ccctcttcga gagtcgtgac tgccgggagg
     1681 gcgtgccgac ggtctacgtc tgtcacgatt tcgtctgcga gctgcccatc accgacccgt
     1741 cgctcctcgg ggtctcgtga gcctcgggcg gtgtcggtgc ccacacgaac acctcgatac
     1801 ggtgacggtg tgcggcaggg ccgtcgggag cgtgcgcggt gtcgccgcct gccgacctgt
     1861 ggccgtactc agtcgcgttc tgccgtgagc accatgttgt cgaccgaggg agccccgctc
     1921 ggcagttcca agcacgtgat catcacgaga cgattcgggg tgtcgttcca cacgaacggc
     1981 agatagggaa ggtcggcctt cttcacgcgg gcccacccgg tgatccggta gtcgacgccg
     2041 tcgacctcga cgagggcgcc gatcgccact ttggcgacgc ccttcgcgac gtctgtcagg
     2101 tagtttcccg gccctatcgc gccaccacga agcgaatgca tggcgacgaa gacggtgccg
     2161 tctcggccgt tcgacggcgt cactcccaag ttgtcgatct ggtaggcgga ggtgaacccg
     2221 ggcggcgtga tctcaccgtc ggtctcgcgc atcgtgccga gcggcaccga cagcccgacc
     2281 gagggcacga cgagcctgcc ggccttgggc cggggggacg cgttcggtgc gtgggtttgg
     2341 ccgggcggcg gagaaggcgt ttttggtgcc acgccctcgc cggggtcgag agtgacgggc
     2401 ttgtggttca tgctcatgct cgtcgcggcc gacgcccggg gttcggagcc cttgatggcg
     2461 gccgtgccga tggcggccag gccgacggcc acgagtacgg cgcagcctcc cagggcgagg
     2521 cggcgccatg gcgacaggcg gtgtcttctc gcctgaggtg cgcccatcag ccccgcaccc
     2581 cgcgaggctc ggatgcgtca tgctgcgcga tatgtgtctc ttgtgacata ggagtttcct
     2641 tccgtgaggt cgggccggct gtccggcccg acctcaccgg cggtcagttc cgcagcgact
     2701 caccgagcct gcgcggattc gcggcgtgga gcccagacga gagcggcgga gacggcgacg
     2761 cccagagtga ggaggcccgc accggccaga aggaggccgt caggttcggt cgtcgaagac
     2821 ggtgccagca ctccgccggt gttgacgcgg gggccgacca cggcttggga gaacacgcgc
     2881 acgccgacgg cgatgttgtt ggtgttattg gggtcgctgg gtgcctgggc agcttggccg
     2941 gccggcgttg ccgcactcgt cgtcgtgtct gccgcctcgc caggctcgga ggcgccggtg
     3001 gaaccgttgg cgtcggagcc tgcggggtct tgcgccgtgc cgggcgtgct gctggcggtc
     3061 gacgggccgc acggcggcat cggcggcgtg gtcgtttgtg gcacgccggt gctcgatgtg
     3121 ttgggggcgg ccggcgcgct tgggcaggcc ggagcgctgg gtgtcgtcgg cgcggacggt
     3181 gtggaggcac ccacgggggc tgctgggctc gcgggggctg ccgggctcgc gggggctgcc
     3241 gggctggcgg gcggagccac ctgcatcggc tgtgctgctt gtgcgactcc catgccgacg
     3301 agtgagaggg gaagggcgat cgccgccgcc agggcatact tagctactga tttcatgctg
     3361 aaaacctcga tgatctttga ccaact
//
LOCUS       DASTNL010000119        13113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_270539, whole genome shotgun sequence.
ACCESSION   DASTNL010000119 DASTNL010000000
VERSION     DASTNL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270539"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFQ96_03740"
     CDS             complement(<1..548)
                     /locus_tag="VFQ96_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEU0256932.1"
                     /translation="MSGEAPVAGDARGAGRVIVVVGAGPRAIGWLERFAANLPLFAPP
                     RVTIHLVDPYPPGAGRVWRLDQSPLVLMNSMAADVTMFTDDSVQMDGPVAPGPSLAEW
                     AAAVRSGRLTHTPVPEDVRREIQTLGPHGFASRRLQGVYLGWVYRRVLASLPEGSVVL
                     DADTAVGVDDLPGGRQLVRLASG"
     gene            complement(545..1939)
                     /locus_tag="VFQ96_03745"
     CDS             complement(545..1939)
                     /locus_tag="VFQ96_03745"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797504.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEU0256933.1"
                     /translation="MRVFETDHTRRAFPLADDHGHTPGGASHGTIQFGVFFQGVNSST
                     IWNTPASGSQTDFESFRRVAQTAERGLFTAFFLGEGLRLREHLGHLHELDVAGRPDAL
                     TQLAALAALTHTIGLVATQNATYNDPIDLARRLQSLDLLSDGRAAWNVVTTDNAWTGE
                     NFRRGGYLDHADRYTHAADVIAIARQVWDARQAGRPAARVHYDTKYYSVDGVGTLPRS
                     RQEHPVLFQAGDSAEGRDFGAANAEVIFSAHSGLDDALEFAADMRRRTVAAGRPDDDL
                     KIFPGQEFIVADTESEVADKVRWVREHQITPQTAIAFVEALWGTDLSGYDPDGPLPAT
                     DPVVQVTGETRGAGFRAAQAAQTAAKWRALAEEHKFSIRELVIHLNSRRGIAGTAEGI
                     ADQLVPYATQHAVDGFNITPWLIPSGLDDIVNKLVPALQERGIYWSEYPSDTLRGNLG
                     LRPPLTRRGEPARP"
     gene            complement(1936..3066)
                     /locus_tag="VFQ96_03750"
     CDS             complement(1936..3066)
                     /locus_tag="VFQ96_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0256934.1"
                     /translation="MTASSPPGTPGFLLLVAIDAAGAHPAAWAEGRPGGHRADEQGTS
                     GAVAALVRAVRAAERAGFTAATFTDQLLPKDAPGVLDAVLRAAHVAPLTGAIGLIPAV
                     QALYAEPFHLATQVASLDTASHGRAGWIVSADPDPRLAALYGRPALPAAEADGELADI
                     VAAARRLWDTWEDDAVIRESATGRYYDRTKVHYADFVGTRFLVKGPAIVPRSPQGQPV
                     VFGPAGTSAEVDAVLVDAPAAPGEPIVAAAAEPLARARANHGAGVRVILDVEVVLDAA
                     GVRAADRLAALDRRAGWAAKTARYVGDAAGLEALLGQLASIADGVRIAPAQTDVDLEE
                     IGRAVLPPLRQAGIVTSPRAGDTLRDSLGLGRPESRYLEVAR"
     gene            3208..3780
                     /locus_tag="VFQ96_03755"
     CDS             3208..3780
                     /locus_tag="VFQ96_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0256935.1"
                     /translation="MTVRVVAVSGTLHAPSKTDVVVRAAVDSAREQLGSAREQASAAA
                     SGGLEAHTIGVLALREELARSLPGGPTPAVQEALDFVRQADVLVVATPVYKGSYTGLL
                     KLFIDFLGRGDIADVPIVLAAIGGSQEHSLVIEHELRPLFGFFSAAPVAEPLYLQPAD
                     FAGEGVTSAAALRIARAVGQALAAATRRAA"
     gene            complement(3829..5631)
                     /locus_tag="VFQ96_03760"
     CDS             complement(3829..5631)
                     /locus_tag="VFQ96_03760"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU0256936.1"
                     /translation="MAEADETEAAFFARVRAWRDQDPDDETRSELTGLLEAAEATDPP
                     AGAGAAAGRSAALAELHARFDTRLAFGTAGLRGELAAGPNRMNRVLVCQAARGLAAYL
                     LEKAGLPEKPGPTETPTPSDRPGPARRPSVVIGYDGRKNSAVFARDTAEIMAGAGIRA
                     ILLPRLLPTPVLAFAVRHLDASAGVMVTASHNPPRDNGYKVYLGGPDAGSQIVAPADG
                     EIARHIGLAANESVARMPRSLDYETADTALVDAYVARAAEAARRLAGDAALGDVRVAY
                     TAMHGVGWATARRVFARAGIPEPAVVAQQIEPDPTFHTVAFPNPEEPGAMDLAFAAGR
                     ETHADLVVANDPDADRLAVAIPDPGPAGYRRLSGNEVGMLLGWAIAERGRGEGVSAHD
                     TLAVSIVSSPALRQVASRYGLGYEETLTGFKWVSRTPRLLYGFEEALGYLVDPDAVRD
                     KDGISAAAVFLTMARLLAARGVTIQDRLDEFARTFGYHASRQLAIRVADPARLATMMR
                     RLRATPPDHVGRSRVRQIDDLADGFEGLPPSDVLRLHLEGGARVVVRPSGTEPKLKVY
                     LDAASEAGSLEERKAAALTLVDDLAGAMAGLLDA"
     gene            complement(5721..6560)
                     /locus_tag="VFQ96_03765"
     CDS             complement(5721..6560)
                     /locus_tag="VFQ96_03765"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU0256937.1"
                     /translation="MADTNPQNNPLDDPGADPFAVAQEAARKISQLTGVAHHDIALTL
                     GSGWGKAADLVGETTATIDAATVPGFSRPALAGHSGTLRSVRLADGRRALVIGARTHF
                     YEGHGVRRVVHSVRTAAACGARTMVLTNGAGGIRPHWAPGTPVLISDHINLTAASPLE
                     GATFVDLTDLYSSRLRGIAREIEPGLDEGVYCQFRGPQYETPAEVRMARAVGGDIVGM
                     STALEAIAAREAGMEVLGLSLITNPAAGITGVPLSHDEVIEAGRRAEPVIGALLARIV
                     ARL"
     gene            6653..8089
                     /locus_tag="VFQ96_03770"
     CDS             6653..8089
                     /locus_tag="VFQ96_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEU0256938.1"
                     /translation="MAFEFERKQSVAVLGGGPGGYEAALAAAQLGADVTLVERSGVGG
                     SAVLTDVVPSKTLIATAEATNGIRNASELGVQFFARSAEGKPIRPEVAVNLAAVNKRL
                     LGLARQQSEDVRTELVHSGVRIVAGEGRLDGPGALIVSTGTGGRGTDFDRIEADTIVV
                     SVGASPRILDTAVPDGERILTWKQLYDLSAIPGELVVVGSGVTGAEFASAYTALGSTV
                     TLVSSRERVLPNEDADAASLIEGVFKREGMTVLSRSRAASVERTDDGVLVTLTDGRQV
                     TGTHCLMAVGSVPNTRGIGLEEAGVQLAPSGHIRVNRVARTSIPNIYAAGDCSDLLPL
                     ASVAQMQGRTAVFHALGDEVKPIELRNVTSNIFTQPEIATVGFSQKQIEDGIAQGDIC
                     KVPLRQNARAKMMGLRDGFVKLFARTGSGTVIGGVIVAPKASELVAPLALAVEHRLTV
                     DQVARAFTVYPSLSGALSDAARAMHIVR"
     gene            complement(8114..9865)
                     /locus_tag="VFQ96_03775"
     CDS             complement(8114..9865)
                     /locus_tag="VFQ96_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU0256939.1"
                     /translation="MPHISKVLVANRGEIAVRVIRAARDSGISSVAVYADQDRGAVHV
                     RLADEAFALEGTTSAETYLAIDKLISIAHRSGADAVHPGYGFLSENAHFAQAVIDAGL
                     IWVGPPPQAIERLGDKVSARHIAEAVGAPLAPGTLNPVSGAAEVLEFADQNGLPVAIK
                     AAFGGGGRGLKVAYTRAEIPELFDSASREAVVAFGRGECFVEKYLDRPRHVETQCLAD
                     AAGGVVVISTRDCSLQRRHQKLVEEAPAPFLTDRQRQMLYTSSKAILRAAGYTSAGTC
                     EFLIGRDGTVSFLEVNTRLQVEHTVSEEVTGIDLVREQFRIAEGGTLDYDDPEPRAHS
                     FEFRINGEDAGRGFLPSPGPVTVFQPPAGPGVRVDSGVVAGDVITGSFDSLLAKLVVT
                     GASRDEALRRARRALAEFEVAGLPTVLPFHRKVVSDPAFAGHDAAQFAVYTDWIEKGF
                     DNDIEPWSGEAAGEPPAASRHRVVVEVDGKRIEVSLPDTLLSGGPVVPAAPRRRVSGP
                     VHSVSGDSVTAPMQATVVKVAVDDGDAVVKGDLVMVLEAMKMEQPLTAHKDGTVSGLD
                     AAVGDTVPSGRLLLNIV"
     gene            complement(9944..10579)
                     /locus_tag="VFQ96_03780"
     CDS             complement(9944..10579)
                     /locus_tag="VFQ96_03780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU0256940.1"
                     /translation="MSFYLASTSPARLATLRAGGVQPVVIPSHVDELAVVARAEAARG
                     RPLAAAEVVAVLAEAKANAVATMPGLDGLVLGCDSVFEVGGTIYGKPHTPEEALRRWR
                     LQRGATGTLFSGHHLIEMRGGTPGASVSEVASAVVTFAGDLDEQEIDDYVATGEPLEV
                     AGAFTIDGLGGPFIRRIEGDPSTVVGLSLSTVRTLVRRLGVSWTSLWARKL"
     gene            10609..11940
                     /locus_tag="VFQ96_03785"
     CDS             10609..11940
                     /locus_tag="VFQ96_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU0256941.1"
                     /translation="MAETAGRSAGEPGDIIELEPSNPAHGGEFVARYEGRVVFVSDAI
                     DGERVEARLVDTTHDGFWRAQTVRVLKPSPYRRAHVWPEAGLERDPSARPGGAEYGHI
                     ALPYQRALKGRVVGEALSRLAGLERVVEVAPAPGDDAAGGLAWRTKVRLHAGPRGFGP
                     YAGRSHRVVEVGSLPLAVPEVAQAAPLPGRGEADERLAGARAVDVVADDGAAVLAYRR
                     GPATGRRRPRAREKWHTLPAGHAVVRRRVAGHSFEVHAAGFWQVHRRAAGVLFDAVTA
                     ALGDLDPSAAHLDLYGGVGLFAAALAAKTGPDGTITSVESADEATRHARHNLARWPRV
                     AAVGAPVEPYLARLGRVASADERARLAAGVVVLDPPRSGAGKAVVGHLVRIAPARVVY
                     VACDPVALARDLGYFRAAGFEVEEVRAFDLFPHTHHVEAVATLRPPERRRR"
     gene            complement(12221..12514)
                     /locus_tag="VFQ96_03790"
     CDS             complement(12221..12514)
                     /locus_tag="VFQ96_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HEU0256942.1"
                     /translation="MSGREQGAGDSSGRGARGDAPQPPVLDASDLVFETPNLTPAEIA
                     ATTAVVLAAAKAQAAATVTKGRDPGAGWRHSVRHTPALRGAGEGQWARQFRAL"
     gene            complement(12511..>13113)
                     /locus_tag="VFQ96_03795"
     CDS             complement(12511..>13113)
                     /locus_tag="VFQ96_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0256943.1"
                     /translation="IGVIANQPNAMAGTLNIEAGEKASRFMRFCDAFGIPIVTLVDVP
                     GYLPGTDQEWSGVIRRGAKLLYAYAEATVPLVTVITRKAYGGAYLVMGSKQLGADLNY
                     AWPSAEIAVMGGQGAVNILYRTELKEAQAQGRDMAEVRAQLAHDYTYNIASPYLAAER
                     GELDSVIEPAATRIMVTKALRTLRTKRRAAVPKKHGNIPL"
BASE COUNT         1705 a         4584 c         4886 g         1938 t
ORIGIN      
        1 ccggaggcca gccgcacgag ctgtcggccg cccggcaggt cgtcgacgcc gaccgccgtg
       61 tcggcgtcga gcacgaccga gccctccggc aggctcgcga gcacccggcg gtagacccag
      121 cccaggtaga cgccctgcag ccggcggctg gcgaagccgt gtgggccaag cgtctgaatc
      181 tcacgccgca catcctccgg caccggagtg tgcgtgaggc ggcccgaccg aacggccgca
      241 gcccattcgg ccagcgacgg gccgggagca acgggcccgt ccatctgcac cgaatcgtcg
      301 gtgaacatcg tgacgtctgc cgccatggag ttcatgagga cgagcggcga ctggtcgagc
      361 cgccataccc ggccggcgcc cggggggtac gggtcgacga ggtggatcgt cacccgaggc
      421 ggggcgaaga ggggcaggtt ggcggcgaaa cgctccagcc agccgatcgc ccgaggcccc
      481 gccccgacga cgacgatcac ccgaccggcg ccccgtgcgt cgccggcgac cggtgcctcg
      541 ccgctcacgg ccgggcgggc tcgcccctgc gcgtcaacgg cgggcgcagg ccgaggttgc
      601 cgcgcagcgt gtccgacggg tattcgctcc agtagatgcc gcgctcttgc agggcgggaa
      661 ccagcttgtt cacgatgtcg tcgagcccgg aggggatcag ccacggtgtg atgttgaagc
      721 cgtcgacggc gtgctgcgta gcgtatggca cgagctggtc ggcgatgcct tcggccgttc
      781 cggcgatgcc gcgtcgggaa ttgaggtgga tgacgagctc ccggatcgag aacttgtgct
      841 cctcggcgag cgcccgccat ttcgccgcgg tctgcgccgc ctgcgcggcg cggaagccgg
      901 cgccgcgggt ctcgccggtc acctggacga cggggtcggt ggccggcagc gggccgtcgg
      961 ggtcgtagcc ggagaggtcg gtgccccaca gcgcctcgac gaacgcgatc gccgtctgcg
     1021 gcgtgatctg gtgctctcgc acccaccgca ccttgtcggc cacctccgat tccgtgtcgg
     1081 ccacgatgaa ctcttggccg gggaagatct tcaggtcgtc gtcgggcctt ccggcggcga
     1141 cggtccttcg gcgcatgtct gcggcgaatt cgagggcgtc gtcgaggccg ctgtgcgcgg
     1201 agaagatgac ctccgcgttc gccgccccga agtcgcgacc ctcggcggag tcgccggcct
     1261 ggaagagcac cgggtgctct tgcctgctgc gcggcagggt tccgacaccg tccacggagt
     1321 aatacttcgt gtcgtagtgc actcgggcgg ccgggcggcc ggcttgtcgc gcatcccaca
     1381 cctgccgggc gatcgcgatc acgtcggccg cgtgcgtgta acggtcggcg tggtcgagat
     1441 agccgccgcg gcggaagttc tcgccggtcc atgcgttgtc ggtcgtcacg acgttccagg
     1501 cggcgcggcc gtcggagagg aggtcgaggc tctgcagccg gcgggcgagg tcgatcgggt
     1561 cgttgtacgt ggcgttctgg gtcgccacga ggccgatcgt gtgggtcagg gcggcgagtg
     1621 ccgccagttg ggtgagcgcg tcgggccggc cggcgacgtc gagttcgtgc agatggccga
     1681 ggtgttccct caggcgcagg ccctcgccca ggaagaaggc ggtgaagagc cctcgctcgg
     1741 cggtctgtgc gacgcgtcgg aagctctcga agtcggtctg tgagccgctg gccggagtgt
     1801 tccagatggt tgacgagttg acgccttgaa agaacacgcc gaactgtatc gtgccgtgcg
     1861 acgcgccgcc cggggtgtgt ccgtggtcgt cggcgagcgg gaatgcccgc cgtgtgtggt
     1921 cggtctcgaa gaccctcatc gtgccacctc caggtagcgg ctttcgggtc ggccaaggcc
     1981 caacgaatcg cgcagtgtgt cgccggcgcg aggcgacgtg acgatgccgg cctggcgcag
     2041 cggggggagg acggcgcggc cgatctcttc gaggtcgacg tcggtctggg cgggggcgat
     2101 ccgcacgccg tcggcgatgc tcgccagctg ccccaggagc gcctcgagcc cggccgcgtc
     2161 gccgacgtag cgggcggtct tcgcggccca cccggcgcgc cggtcgagcg cggccagccg
     2221 gtcggcggca cgcacgccgg cggcgtcgag cacgacctcc acgtcgagga tcacgcgcac
     2281 gcccgcgccg tggttcgccc tcgcccgggc gagaggctct gcggcagccg cgacgatggg
     2341 ctcgcccggt gcggcggggg cgtcgaccaa caccgcgtcg acctctgccg acgtgccggc
     2401 ggggccgaac acgacgggtt gaccctgcgg cgagcggggc acgatcgccg ggcccttcac
     2461 taagaaccgg gtgcccacga agtcggcgta gtgcaccttg gtgcggtcgt agtaacgccc
     2521 cgtggcgctc tcgcggatga ccgcgtcgtc ttcccacgtg tcccacagcc ggcgtgccgc
     2581 ggcgacgatg tcggcgagtt ccccgtcggc ctcggcggca ggcagtgccg gccggccgta
     2641 aagcgcggcg aggcgggggt cggggtcggc ggagacgatc cagccggcac ggccgtgact
     2701 ggccgtgtcg aggctcgcca cctgcgtggc gaggtggaac ggttccgcat acaacgcctg
     2761 cactgcgggg atcaacccga tcgcgccggt gagcggtgcg acatgagcgg cccgcagcac
     2821 cgcgtcgaga acgcccggcg cgtctttcgg cagcagctgg tcggtgaagg tcgccgccgt
     2881 gaatcctgcc cgttcggccg cgcgcaccgc ccgcacgagc gctgcgacgg cgcccgacgt
     2941 gccctgctcg tcggcccggt ggccgccggg ccggccttcc gcccacgcgg ctggatgcgc
     3001 acctgctgcg tcgatcgcga cgaggaggag gaatccggga gtgccaggtg gggaagatgc
     3061 cgtcatggca cgaccataag gcgacaccgt ggcggcggac cggcgtcggc gtcacagtgc
     3121 gcaacatgat cacgccgcgc cgggtggcgg ggccagggtg gaagaggcgg gccgcaccgg
     3181 gcaggccttg tgaggggagt tcgtgagatg acggttcggg tcgtcgccgt gtcgggtacg
     3241 ctgcacgcgc cgtcgaagac ggatgtcgtc gtgcgggcgg cggtcgattc cgctcgcgaa
     3301 cagctgggtt ctgcgcgtga gcaggcctct gctgccgcct ccgggggtct tgaggcgcac
     3361 acgatcggcg tgttggcgtt gcgggaggag ctggcacgtt ccctgcccgg cgggcccacc
     3421 ccggcggtgc aagaggcgct cgacttcgtg cgccaggccg acgtgctcgt cgtcgcgacg
     3481 cccgtctaca agggcagcta cacgggcctt ctgaagctgt tcatcgactt tctcggccgc
     3541 ggcgacatcg ccgacgttcc catcgtcctc gcggcgatcg gtggcagcca ggaacattcc
     3601 ctcgtcatcg agcacgagct gcggccgctc ttcggcttct tctcggccgc cccggtcgcc
     3661 gagcctctct acctacagcc tgccgacttt gcgggggagg gcgtcacctc ggccgcagcg
     3721 ttgcgcatcg cgcgggccgt cggccaggcg ctcgccgccg ccacacggcg cgccgcctga
     3781 ccgccggccg ccgcccggcc tcgtgcggcc ctgcccgcct tgccgtgctc aggcgtcgag
     3841 aaggccggcc atggcgccgg ccaggtcgtc gacgagggtg agcgcggccg ccttgcgctc
     3901 ctcgaggctg cccgcctcgc tcgccgcgtc gaggtagacc ttgagcttcg gctcggtgcc
     3961 gctcggccgc acgacgacgc gcgctccgcc ctccaggtgg aggcgcagca cgtcgctggg
     4021 cggcaggccc tcgaagccgt cggcgagatc gtcgatctgc cgcacgcggc tgcgaccgac
     4081 gtggtccggc ggcgtggcgc gcagccggcg catcatggtg gcgaggcggg ccgggtcggc
     4141 cacgcggatc gccagctgcc gggaggcgtg atagccgaac gtgcgagcga actcgtcgag
     4201 tcggtcttgg atcgtcacgc cgcgggctgc cagaagccgc gccatcgtga ggaacacggc
     4261 cgccgccgag atgccgtctt tgtcgcgcac cgcgtcgggg tcgacgaggt agcccagagc
     4321 ctcctcgaag ccgtagagca gccgaggcgt gcgggagacc cacttgaagc cggtcagcgt
     4381 ctcctcgtac cccaggccgt atcggctggc gacctgccgc aaggcggggg aggagacgat
     4441 ggagaccgcg agggtgtcgt gtgcgcttac gccttcgccc cggccgcgtt ccgcgatcgc
     4501 ccagccgagg agcatgccga cctcgttgcc gctcagccgg cgatagccgg ccggccccgg
     4561 gtcgggaatc gccaccgcga gccggtcggc gtcggggtcg ttcgccacga cgaggtcggc
     4621 gtgggtctcg cggcccgccg cgaaggccag atccatcgcg cccggctcct ctgggttcgg
     4681 gaacgcgacg gtgtggaagg tcgggtcggg ttcgatctgc tgggcgacga ccgccggttc
     4741 ggggatgccc gcccgggcga aaacgcgccg ggcggtcgcc cagcccacgc cgtgcatcgc
     4801 ggtgtaggcg acccgcacgt cgccgagcgc ggcatcgccc gccaggcgtc gggcggcctc
     4861 ggcggcgcgg gccacgtagg cgtcgacgag cgcggtgtcg gccgtctcgt agtcgaggct
     4921 ccgcggcatc cgggcgacgc tctcgttcgc cgccaggccg atgtgccggg cgatctcccc
     4981 gtcggcgggc gccacgatct gagagcctgc gtcgggtccg cccaggtaca ccttgtagcc
     5041 gttgtcgcgg ggcgggttgt ggcttgccgt caccatgacg ccggccgagg cgtcgaggtg
     5101 acgcacggcg aaggcgagca cgggggtggg cagaagccgc ggcagcagaa tggcgcgtat
     5161 gccggcgcct gccatgatct ctgccgtgtc ccgggcgaag acggcggagt tcttgcggcc
     5221 gtcgtagccg atcacgaccg acgggcgccg ggcagggccg ggcctgtcgg agggcgtcgg
     5281 cgtttcggtc gggccgggct tttcgggcag cccggccttt tccagcaggt aggcggccag
     5341 gccccgcgca gcctggcaga cgagcacccg gttcatgcgg ttcgggccgg cggccagttc
     5401 gccgcgcagg ccggccgtgc cgaaggcgag ccgggtatcg aagcgtgcgt gcagctcggc
     5461 caaggcggca gagcgccccg cggccgcgcc cgccccggcc ggcgggtccg tggcctccgc
     5521 ggcctcgagc agcccggtca gctccgaccg ggtctcgtcg tcggggtctt gatcccgcca
     5581 ggcgcgcacc cgcgcgaaga acgccgcttc tgtctcgtcg gcctctgcca tctcgtgtcc
     5641 cttcaggctt ttcgcccttg ttgtcaggcc ggtgcttcct tctttgcttt ggcgagccgg
     5701 gcccgcccag gcgcccgggg tcagagtcgc gcgacgatgc gggccagaag cgcgccgatc
     5761 accggctccg cccgccggcc ggcctcgatc acctcgtcgt ggctgagcgg cacgcccgtg
     5821 atgccggccg ccgggttcgt gatgagagaa aggccgagaa cctccatgcc cgcctcgcgg
     5881 gcggcgatcg cctccaacgc cgtcgacatg ccgacgatgt cgccgcctac cgcccgcgcc
     5941 atgcgcacct ctgccggcgt ctcgtattgc ggcccgcgga actgacagta gacgccctcg
     6001 tcgaggccgg gctcgatctc gcgggcgatg ccgcgcagcc tcgacgaata caggtcggtc
     6061 aggtcgacga acgtcgcccc ttccagcggc gaggcggccg tcaggttgat gtggtcgctg
     6121 atcagcaccg gcgtgccggg cgcccagtgt gggcggatgc cgcccgcgcc gttggtgagc
     6181 accatggtgc gggccccgca agcggccgcc gtgcgcacgc tgtgcaccac gcggcgcacc
     6241 ccgtggccct cgtagaagtg tgtgcgggcg ccgatgacga gggcgcgccg gccgtctgcc
     6301 aggcgcacgg aacgtagggt gccgctgtgc ccggccaggg ccggccggct gaagcccggc
     6361 accgtggccg cgtcgatcgt cgccgtcgtc tcgccgacga ggtcggcggc cttgccccat
     6421 ccgctgccca aggtgagggc gatgtcgtgg tgcgccacgc ccgtcagctg cgagatcttc
     6481 cgggcggcct cctgggccac ggcgaagggg tcggcaccgg ggtcgtcgag cgggttgttc
     6541 tgcgggttcg tgtcagccac gtccgtagtc taaattgcgc gcccgccggg gccgcccgcc
     6601 cgcccggccg cgtcggttcg cgcagcctgc tcatgttagc gagaatgggg caatggcctt
     6661 cgagttcgag cgaaaacaat ccgtcgcggt tctcggaggt ggtcctggag ggtacgaggc
     6721 tgcgctcgcg gccgcgcagc tcggcgccga cgtgacgctc gtggagcgca gcggcgtggg
     6781 cggctccgcg gtcttgaccg atgtcgtgcc gtcgaagacc ctcatcgcca cggcggaggc
     6841 gacgaacggc atccgcaacg cctccgagct gggtgtgcag ttcttcgcgc gctcggcgga
     6901 gggcaagccg atacgccccg aggtcgccgt gaacctggcc gccgtcaata agcgcctgct
     6961 cgggctggcc cgccagcagt cggaagacgt acgcaccgaa ctcgtgcatt ccggggtgcg
     7021 catcgtcgcg ggcgagggcc ggctcgacgg cccgggcgcc ctcatcgtct cgaccgggac
     7081 gggtggtcgc ggcaccgact tcgatcggat cgaggcagac acgatcgtcg tctccgtcgg
     7141 cgcgagcccg cgcattcttg acacggcggt gccggacggc gaacggatcc tcacctggaa
     7201 gcagctttac gacctgagcg ccattcccgg cgagctcgtc gtcgtcggct ccggcgtgac
     7261 cggcgcggaa ttcgcctctg cctacaccgc gctcggctcg accgtgacgc tggtctcgag
     7321 ccgggagcgg gtgttgccga acgaagacgc cgacgcggcc tcgctcatcg agggcgtctt
     7381 caagcgcgag ggcatgaccg tgctctcccg ttcccgcgcc gcctcggtgg agcgcaccga
     7441 cgacggggtg ctcgtcacct tgaccgacgg gcggcaggtg accggcacgc actgcctcat
     7501 ggcggtcggc tcggtgccga acacgcgagg catcgggctg gaagaggcgg gggtgcagct
     7561 ggcgccctcc ggccacatcc gcgtcaaccg ggtggcccgc acctcgatac cgaatatcta
     7621 cgcggcgggc gactgctccg accttctgcc cttggcgtcg gtggcgcaga tgcagggccg
     7681 caccgccgtc ttccacgcgt tgggcgacga ggtgaagccg atcgagctgc gcaacgtcac
     7741 ctcgaacatc ttcacgcagc ccgagatcgc gaccgtcggc ttcagccaga agcagatcga
     7801 ggacggcatc gcgcaaggcg acatctgcaa ggtgccgttg cgtcagaacg ccagggccaa
     7861 gatgatgggc ctgcgcgacg gtttcgtgaa gctcttcgcg cgcaccggca gcggcacggt
     7921 catcggcggg gtcatcgtgg cgcccaaggc gagcgagctg gtcgctccgc tcgcgctcgc
     7981 ggtggagcat cgcctgacgg tcgaccaggt cgcccgggcc ttcaccgtct acccgtcgct
     8041 ctcgggcgca ctctccgacg cggcccgtgc tatgcatatc gtgcgctgac cggccggcgg
     8101 gccgcccggg ccctcagacg atgttgagga gaagccgccc ggaaggcacg gtgtcgccga
     8161 cggcggcgtc gaggccggag acggtgccgt ccttgtgggc ggtcagaggc tgctccatct
     8221 tcatggcctc caacaccatc acgaggtcgc ccttgacgac cgcgtcgcca tcgtcgactg
     8281 cgaccttcac gacggtggcc tgcatcgggg cggtcacgga gtcgccgctg acactgtgca
     8341 cggggccgga gacccggcgg cgcggcgcgg ccggcacgac ggggccgccg ctgaggaggg
     8401 tgtcgggcaa gctcacctcg atgcgtttgc cgtcgacctc gacgacgacg cggtgccgcg
     8461 acgcggcggg cggctccccg gcggcctcgc cgctccacgg ctcgatgtcg ttgtcgaagc
     8521 ccttctcgat ccagtcggtg tagacggcga actgcgccgc gtcgtgcccc gcgaacgcgg
     8581 ggtccgagac caccttgcgg tggaacggca gcacggtcgg caggcccgcc acctcgaact
     8641 cggcgagggc gcggcgggcg cggcgcagcg cctcgtcgcg gctcgcgccc gtcacgacca
     8701 gcttcgcgag cagcgagtcg aaggatccgg tgatgacgtc gcccgcgacg acgccgctgt
     8761 cgacgcgcac gccggggccg gcgggcggct gaaagacggt gacggggccg ggcgacggca
     8821 ggaacccgcg cccggcgtct tcgccgttga tgcggaactc gaacgagtgg gcgcgcggct
     8881 cggggtcgtc gtagtcgagc gtgccgccct ccgcgatgcg gaactgctcg cgcacgaggt
     8941 cgatgcccgt gacctcctcc gagacggtgt gctccacctg cagcctggtg ttgacctcaa
     9001 ggaacgacac ggtgccgtcc cgcccgatga ggaactcgca ggtgccggcg ctcgtgtagc
     9061 ccgccgcccg aagaatcgcc ttcgaggagg tgtacagcat ctgcctctgc cggtcggtca
     9121 agaacggcgc cggcgcctct tcgacgagct tctgatgccg gcgctgcagg gagcagtcgc
     9181 gggtggagat gacgacgacg cccccggccg catccgccag gcattgggtc tcgacgtgcc
     9241 gtggccggtc aaggtatttc tcgacgaagc attcgccgcg gccgaacgcg acgaccgcct
     9301 cgcgggaggc cgagtcgaag agctccggaa tctctgccct tgtgtaggcg accttgaggc
     9361 cgcggccgcc gccgccgaac gccgccttga tcgcgacggg cagcccgttc tggtcagcga
     9421 actccagcac ctcggcggcg ccagagacgg ggttcagggt gccgggcgcc agcggcgccc
     9481 cgaccgcctc ggcgatgtgc ctcgcggaga ccttgtcgcc cagccgctcg atcgcctgcg
     9541 gcgggggacc gacccagatg aggccggcgt cgatgacggc ctgggcgaaa tgcgcgttct
     9601 cggagaggaa gccgtagccc gggtgcacgg cgtcggcgcc gctgcggtgg gcgatggaga
     9661 tcaacttgtc gatcgcgaga taggtctcgg cgctggtcgt gccctccagc gcgaaggcct
     9721 cgtcggcgag acgcacgtgc accgcgccgc ggtcttggtc ggcgtagacg gcgacggatg
     9781 agattccgct gtccctggcc gcccggatga cccgcacggc gatctctccc ctgttggcga
     9841 ccagaacctt ggaaatatgt ggcacgaacc tcagcctatg ggcccggcac gccacctttt
     9901 tcaaaggacg tcgacaaagc tatccgtgaa cgttgtgaag tgtctacaac tttctcgccc
     9961 agagggatgt ccacgagacg ccgagccggc gcacgagggt gcgcaccgtc gagagggaga
    10021 gccccaccac ggtgctggga tccccctcga ttcggcgaat gaacggcccg cccaggccgt
    10081 cgatcgtgaa cgcgccggcc acctcgagag gctccccggt ggcgacgtag tcgtcgatct
    10141 cctgttcgtc gaggtcgccc gcgaacgtca cgacggcgct cgccacctcg ctgacgctgg
    10201 cgcccggggt gccgccccgc atctcgatca ggtggtggcc ggagaagagc gtgcccgtcg
    10261 caccgcgttg cagccgccac cggcgcagcg cctcttcggg ggtgtgcggc ttgccgtaga
    10321 tcgtgccgcc gacctcgaag accgagtcgc agccgagtac gaggccgtcg aggccgggca
    10381 tcgtcgccac ggcgttcgcc ttcgcctcgg ccaacaccgc gacgacctcg gccgccgcga
    10441 gagggcggcc gcgcgccgcc tcggcccttg ccacgacggc cagctcgtcg acgtgcgacg
    10501 ggatgacgac cggctgcacg ccgcccgccc gcagggtcgc caggcgcgca ggcgaggtcg
    10561 aggcgaggta gaaggacatc gggccgttcg ttgtgtcatg ctcgacgggt ggccgagaca
    10621 gcggggcgaa gcgccggtga acccggcgac atcatcgagc tcgagcctag caaccccgcg
    10681 cacggcggcg agttcgtggc gcggtacgag gggcgggtcg tgttcgtctc tgacgcgatc
    10741 gacggggagc gggtcgaggc gaggctcgtc gacacgacgc acgacgggtt ctggcgggcc
    10801 cagaccgttc gggtcctcaa gccgtcgccg taccgccggg cgcacgtgtg gccggaggcc
    10861 ggcctcgagc gcgatccctc ggcgcggccg ggcggcgccg aatacggcca tatcgccctg
    10921 ccctatcaac gggcgctgaa ggggcgggtc gtgggcgagg cgctttcgcg cctcgccggc
    10981 ctggagcggg tcgtcgaagt ggcccccgcc ccgggggacg acgcggccgg cggcctggcg
    11041 tggcgcacca aggtgcgcct gcatgccggc ccgcgcgggt tcgggccgta cgccggccga
    11101 tcccaccggg tcgtcgaggt cggctccttg ccgctggcgg tgccggaggt cgcgcaggcg
    11161 gcgcccctgc cggggcgggg cgaggccgac gagcgcctgg cgggcgcccg ggccgtcgac
    11221 gtcgtggccg acgacggtgc cgcggtgctg gcctaccgcc ggggccccgc caccggccgg
    11281 cgccggccgc gcgcgcggga gaaatggcac acccttccag ccggacacgc cgtcgtccgg
    11341 cgccgggtgg cggggcattc gttcgaggtg cacgccgccg gcttctggca ggtgcatcgc
    11401 cgggcggccg gggtgctctt cgacgcggtg accgcggcgc tcggagacct cgacccgagc
    11461 gccgcgcacc tcgatcttta cggcggggtg gggctcttcg ccgcggcgct ggccgcgaag
    11521 acgggccccg acggcacgat cacgtccgtc gaatccgccg acgaggcgac acggcacgct
    11581 cggcacaacc tggcgcgctg gccgagggtg gcggcggtcg gggccccggt cgagccttac
    11641 ctcgcacggc tcgggcgggt ggcctccgcc gacgaacggg cgcgccttgc ggcgggggtc
    11701 gtcgtgctcg acccgccgcg cagcggggcc ggcaaggccg tggtcgggca cctggtgaga
    11761 atcgcacccg cccgcgtcgt gtatgtggcc tgcgacccgg tcgcccttgc tcgtgacctg
    11821 gggtactttc gtgcggcggg gttcgaggtg gaggaggtgc gcgcgttcga cctctttccc
    11881 cacacgcatc acgtcgaggc cgtcgcgacc ctccgcccgc ccgagcgtcg ccggcggtga
    11941 ccggccggtc ggcgcccgca aacgggttcc ggatcggagc gagagaaggg ccggggtggt
    12001 aacccgaata ccaccccggc cggccgatcg cagggcggct tctcagcctg gatgaccccg
    12061 ctcggctggc ggcctagatg ccgccgtgtc cttactatag tgcgccgcag gggccgttct
    12121 gtaaagggtc ttttcgcctt ctttaccggt cggatccacg ctgtcgtgca gggccgagaa
    12181 gcgtggctcg cgggcctttc ccgtcgggtc gggcggcggg tcagagcgcc cggaattgcc
    12241 gggcccattg cccctcgccc gcgccccgaa gggccggcgt gtggcgcacg ctgtgccgcc
    12301 acccggcccc cgggtcgcgg cctttcgtga cggtggccgc ggcctgggcc ttcgccgccg
    12361 cgagaacgac agccgtggtg gctgcgatct cggccggcgt gaggttcggc gtctcgaaga
    12421 ccaggtcgga ggcgtcgagc actggcggct gcggcgcgtc gcccctggcc ccgcggcccg
    12481 aagagtcgcc cgccccctgc tcgcggccgc tcatagcggg atgttcccgt gcttcttcgg
    12541 caccgcggcg cgtcgtttcg tgcgcagcgt gcgcagcgcc ttggtgacca tgatgcgggt
    12601 cgcggccggc tcgatgacgc tgtcgagctc gccgcgctcc gccgcgaggt aggggctcgc
    12661 gatgttgtag gtgtagtcgt gtgcaagctg cgcccgcacc tcggccatgt cgcggccctg
    12721 ggcctgcgcc tctttcagct cggtgcggta gaggatgttc actgcgccct gcccgcccat
    12781 cacggcgatc tcggcgctcg gccaggcgta gttgaggtcg gcgccgagtt gcttggagcc
    12841 catgacgagg taggccccgc cgtacgcctt gcgcgtgatg acggtgacga gcggcaccgt
    12901 cgcctcggca tacgcgtaga gcagtttcgc gccacggcgg atcaccccgc tccactcttg
    12961 gtcggtgccg ggcaggtagc cgggcacgtc gacgagggtg acgatgggga tgccgaacgc
    13021 gtcgcagaac cgcatgaacc gcgacgcctt ctcgccggcc tcgatgttga gcgtgcccgc
    13081 catcgcgttc ggctggttgg cgatgacgcc gat
//
LOCUS       DASTNL010000120         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1011637, whole genome shotgun sequence.
ACCESSION   DASTNL010000120 DASTNL010000000
VERSION     DASTNL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011637"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..724)
                     /locus_tag="VFQ96_03800"
     CDS             complement(95..724)
                     /locus_tag="VFQ96_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256944.1"
                     /translation="MTRRHRTWRRWLVVAYIVLAVAGGVLILIGDLVGAAHGNEGQGI
                     SAPGVWQDYTGAATFILASMLAAVHMIVAARDTYVARSHDEIEAARARRAAERKERAA
                     WVAIQRQRRADLKAAKPQRSDAENALMAAWVGSGVLAVILIIAGVALLANSSSSLDGT
                     TVHGIGELVWGGSFASIFIIAGAAHLAVAAVKHEIKEAVENVTSRLPEA"
     gene            complement(721..1071)
                     /locus_tag="VFQ96_03805"
     CDS             complement(721..1071)
                     /locus_tag="VFQ96_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256945.1"
                     /translation="MGLFNSGSNQAYEQYLPVPPKKAFEALCQAVDSGSQFTLKEADD
                     FALTAIFTSGISLATYGQRFNAQIVPSGDASSLRVTAVAKVGGSLFNAARAQKLVDAV
                     FSGASDLLRPQARR"
     gene            1719..2207
                     /locus_tag="VFQ96_03810"
     CDS             1719..2207
                     /locus_tag="VFQ96_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256946.1"
                     /translation="MSTNDATTYGQQGAWTDRRSRAFLILGIVAVVGGGLLSAIIAPT
                     PSYVGSWAVAYIVLVGGAAQVALGVAQASVARRPLTRGILLTQVLLLNLGVAGTVVAT
                     VANIPVLLYLSAAAQVAAVIFLLAATRKGRPGAALITLRVVAIVLLIGSPTGVILQAV
                     AS"
     gene            2283..3464
                     /locus_tag="VFQ96_03815"
     CDS             2283..3464
                     /locus_tag="VFQ96_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0256947.1"
                     /translation="MRYRLTALRRLAARQAIGLRSRLWRAGHRTGYQQGLLSVVVPVW
                     GVEKYLGRCLDSLRAGAYRNLEIVVVDDGSPDGSAAVARGRRWRDPRIRLIRRPNGGL
                     SAARNTGIRAARGEFLAFLDGDDFVDQGAYAAAIRSLHKTGSDFAVLPYRRWRAGAAH
                     HPAPWIRAAHRRGRARLTLSEAPWVLVDAVAWNKVYRREFWQRQGLSFPPGLLYEDQA
                     VSMTVFAAARRFDVLAGPGVMWRLRDDRSSITDDKAHVRNLADQERAARDSLDVLARL
                     STPDVRRERLRQLLNNNLSGFIEHIPDMAPAAWAAFRSLCRYLADEADRDDTGGIWGG
                     VEARKKIVVALARADRCDLAEAFLAAGGWAAANPAAPPEVAAACDERMLRPGHPPAVP
                     A"
BASE COUNT          563 a         1174 c         1194 g          595 t
ORIGIN      
        1 ggcgatgatc gggtgggcga agccgacgcc gatggtgccg aggaacagga cgatgatgac
       61 gggaagggct gcagcgtcga gcctgcgtgg ctggctacgc ctccggtaga cgtgacgtga
      121 cattctcgac cgcctccttt atctcatgtt tcacggcggc gacggcaaga tgtgcggcgc
      181 cggcgatgat gaagatgctg gcgaaggaac ctccccacac gagttctcct atgccgtgaa
      241 ccgttgtgcc atccaggctg gagctcgagt ttgcaaggag tgcgacgcca gcgattataa
      301 ggatgactgc gagaacgcca ctgccgaccc aggcagccat gagagcgttc tcggcgtcgc
      361 ttcgctgggg ctttgccgct ttcaggtcgg ctcgtcgttg gcgttggatg gcgacccatg
      421 cggctcgttc ttttctctcg gccgcgcgac gtgctctggc tgcctcgatc tcgtcgtgtg
      481 acctggccac gtaagtgtct cgtgcggcga cgatcatgtg cacggcggcg agcatgctgg
      541 cgagaatgaa ggtcgcggca ccggtgtaat cctgccagac acccggcgcg ctgatccctt
      601 ggccttcgtt accgtgtgcg gcgccgacga gatcaccgat aaggatgagc acgccgccag
      661 ctacagcgag gacgatgtaa gcgacgacga gccaacgcct ccacgtgcgg tgacggcgag
      721 tcaacgtcgt gcctgagggc gtaggagatc ggacgcgcct gagaagaccg cgtcgacgag
      781 cttctgtgcg cgtgccgcgt tgaacagtga ccctccgacc ttcgcgacgg cggtgacgcg
      841 gaggctcgat gcgtctcctg acggtacgat ctgggcgttg aagcgttgcc cgtaggtggc
      901 gagggaaatg ccgcttgtga agatcgcggt gagtgcgaag tcgtcggctt ctttgagggt
      961 gaactgggac ccgctgtcga cagcctggca gagtgcttcg aatgctttct ttggggggac
     1021 ggggaggtat tgctcgtagg cctggttgga gccggagttg aagagtccca ttgtcgcctt
     1081 cctgttctgt gggtccacga ttccactgag gcgattcgct gtcgaggtga gtcctcggaa
     1141 gctgtcgggt ggctctccgt ttactctcct ttttcacatg ctcactggct gtaactgggg
     1201 atcactggag agtgccgacc cgtttagatg taacacacgg tagctgcagg taaatgacat
     1261 aggagaatct cagctctcca ggctggtggc gcgggttcga ttcccgtcac tcgctctgac
     1321 gatgaaaccc caggtcacag cgtggttctg aggttctacc gacagcaagg cgaaggcgag
     1381 ccaagcccct ttcgtattct ctgtgggctc acgggtcgac gagaggtacg atcgtggccg
     1441 aaacgaccgc cctccggtag ccgctcaaac ggcattttca ccggcgcgct gggcgtgccg
     1501 cacccaccga ggccaccgct tcagacgcca gactggtgtc atcacgaatc catccgggtg
     1561 cgcacgaacg acgacggcgg ggaagagctc tccgcggtga ggaacgaatc catgacaacg
     1621 actatcagcg ccggcagcga cacgacgagg cgttcgcgac gacctgacgg accgcgctct
     1681 tctaccgccg aaggtgccgg agggaaaggg ctgtcccggt gagcacgaac gacgccacga
     1741 catacggtca acagggcgct tggacggaca ggcgctcccg cgcgttcctc atcctcggca
     1801 tcgtcgccgt ggtgggcggc gggctcctct ccgccatcat cgccccgacc cccagctacg
     1861 tcggcagctg ggccgtcgcc tacatcgtct tggtcggcgg cgccgcccag gtcgccctcg
     1921 gggtggcgca ggcgtcggtc gcccggcgcc cgctgacacg cggcattctt ctcacacagg
     1981 tgctgctctt gaacctcgga gtcgccggta cggtcgtggc gaccgtcgcc aacattcccg
     2041 tcctcctcta cctgtcggcc gcagcacagg tggcggccgt gatcttcctc ctggccgcca
     2101 cgcgaaaggg gcggcccggg gcggccctca taaccctccg cgtggtggcg atcgtcctcc
     2161 tcatcggctc gcccaccgga gttatcctcc aagccgtcgc gtcgtagcca cgccgggcac
     2221 ccgggcatcg acacgggaaa tcaggcggcg gcccggccgg cgccgccttg aggggagacc
     2281 gcatgaggta ccgcctgacg gcgctgcgac ggctggccgc gcgccaggcg atcgggctcc
     2341 gcagccgcct ctggcgggcc gggcatcgaa cgggctacca gcaaggcctt ctctccgtcg
     2401 tcgtaccggt gtggggggtc gagaagtacc tcggccggtg cctcgactcg ctgcgggccg
     2461 gcgcctaccg caatctggag atcgtcgtcg tcgacgacgg ctcccccgat gggagcgccg
     2521 cggtggcgcg cgggcggcgg tggcgagacc cgcgcatccg gctcatccga cgcccgaacg
     2581 gcgggctctc cgcggcgcga aacaccggca tccgggctgc gcgaggcgag tttctcgcct
     2641 ttctcgacgg cgacgacttc gtcgaccaag gtgcctacgc ggccgcgatc cggtcgttgc
     2701 acaagaccgg atcggatttt gccgtactgc cctaccgccg ctggcgggcc ggggcggcgc
     2761 accacccggc gccatggatc cgcgcggccc accgccgcgg ccgggcccgc ttgacgctct
     2821 ccgaagcccc gtgggtgctg gtggacgccg tggcctggaa caaggtgtac cggcgagagt
     2881 tctggcagcg gcagggcctc agcttcccgc ccggcctgct ctacgaagac caagccgtct
     2941 cgatgaccgt gttcgcggcc gcgcgccggt tcgacgtgct ggccggcccc ggcgtcatgt
     3001 ggcggctgcg cgacgaccgc tcctcgatca cggacgacaa ggcccacgtc agaaacctcg
     3061 ctgaccagga acgggccgcc cgcgacagcc tcgacgtgct cgcaaggctc tcgacacccg
     3121 acgttcggcg agaaaggctt cgccaactgc tgaacaacaa cctgagcggc ttcatcgagc
     3181 acatccccga catggcccct gccgcctggg ccgcgttccg cagcctctgc cgctacctgg
     3241 ccgacgaggc cgaccgcgac gacacaggcg gcatctgggg cggggtggag gcacgcaaaa
     3301 aaatcgtcgt cgccctcgcc cgtgccgacc ggtgtgacct ggccgaggcg tttctcgccg
     3361 ccggcggctg ggccgccgcg aacccggccg cgccgcccga ggtcgcggcc gcctgcgacg
     3421 agcggatgct acggccgggc cacccgcctg ccgtgcccgc ataagacggc gaccgcgggg
     3481 cgcgaaggcg cgtcactccg aggaggagtc gtcgaacgac acgtat
//
LOCUS       DASTNL010000121         9600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_458255, whole genome shotgun sequence.
ACCESSION   DASTNL010000121 DASTNL010000000
VERSION     DASTNL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458255"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFQ96_03820"
     CDS             <1..485
                     /locus_tag="VFQ96_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0256948.1"
                     /translation="RGLKGWATRQFMLATGQIPIDRSGGKASEAALNTGMRVLAAGQV
                     LGIYPEGTRSPDGRLYRGRTGIARMILEAKVPVVPVVMVGTREMMPIGARLPKIRRVG
                     MVVGEPLDFSRFEGMENDRFVLRSITDEIMYALRTLSGQEYVDLYASTVKERRAALSR
                     "
     gene            588..1961
                     /locus_tag="VFQ96_03825"
     CDS             588..1961
                     /locus_tag="VFQ96_03825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEU0256949.1"
                     /translation="MQATEPVVAPDKDVIEGLDYWRSLPIKQQPPWPDEAAVHAASAE
                     LATLPPLVFAGEVDQLRQRLAKAAQGEAFLLQGGDCAETFAGATADQIRDRVKTVLQM
                     AVVLTYGASVPVIKLGRMAGQFAKPRSRDTETRGGVTLPAYRGDIVNGYDFTPESRRA
                     DPSRMLAGYHTAASTLNLIRAFTQGGFADLREVHSWNRGFASNPANRRYEGMAHEIDR
                     AIKFMQAAGADFDELKRVEFYSSHEALLMDYERPLTRIDSRTGLPYDTSAHLVWVGER
                     TRDPEGAHVDFLSRVRNPIGVKLGPSTTGDAMMRLVDKLDPEREPGRLTFITRMGAGV
                     IRDALPPLLERAKSEGITPLWVTDPMHGNGMTTPAGYKTRRFDEIIDELRGFFAVHRE
                     VGTNPGGIHVELTGDDVTECLGGSEHIDEESLETRYESLCDPRLNHMQSLELAFLVAE
                     ELRATQY"
     gene            complement(2020..3948)
                     /gene="pknB"
                     /locus_tag="VFQ96_03830"
     CDS             complement(2020..3948)
                     /gene="pknB"
                     /locus_tag="VFQ96_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU0256950.1"
                     /translation="MTTAASDPMIGRLVDGRYRVRSRIARGGMATVYLATDLRLERQV
                     AIKVMHSFLADDAALKDRFVQEARSAARLAHPNVVNVFDQGQDDDMAYLVMEYLPGIT
                     LRDLLRERGRLTGAQAVGIGVAVLSGLAAAHRAGIIHRDLKPENVLLCDDGRIKIGDF
                     GLARAASAATASGQLLLGTIAYLSPELVTRGVADTRSDIYAMGIMLYEMLTGEQPFHG
                     EEPVQIAYQHANDSVPPPSLANPAVSPELDELVLWATAKSPDARPADAQVLLDQLSDI
                     GSTTSGEVSPQATLVLPDAGATAVLTAPIAAPGRLRGRPAIPGSSGNRASGVFGPGAV
                     LAKRASRRKLRGWWVFVLVVVLAAASGGAGWYFGSGPGAFVSLPGLAGASPTRATAAL
                     TRLGLDVGQRTEHSLRVTRGTVIATSPRAGTDVRRGSKVVLRVSSGPAPVTLPKLAGM
                     TAAQATKALAALNVTVLPDRDEVFDKTQAGTVLQARMGARDVSAGGAAHEGEKVALVV
                     SLGPVPDVVGESESAASGALNAAGLEVGPESNSYSDTVPKDHVISAAPASNPPLHKGD
                     AIILTISKGPQQVPVPDVTGMSIKDAEKTLAQAGFHVNVKTNVPKPLWSVIDPVSTDP
                     PAGTPVDKGAKVTINAQY"
     gene            4034..4369
                     /locus_tag="VFQ96_03835"
     CDS             4034..4369
                     /locus_tag="VFQ96_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HEU0256951.1"
                     /translation="MTFVQDSWLTLDDAARMLSVGVGRVRRLIEEHRLAAIRVQGKAR
                     IPQGFVRDGKPLPELRGTLIALSDAGFDNDEAVDWLLRKNDDLDATPLEALRDGRSKM
                     VRWNIQTLG"
     gene            complement(4478..5566)
                     /locus_tag="VFQ96_03840"
     CDS             complement(4478..5566)
                     /locus_tag="VFQ96_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0256952.1"
                     /translation="MAQSTTLTDLVRDRVVAHLAARTPILLGVAPDLAAVTEFSRQFL
                     GGGKRFRARFCHLGWASGKGDGDGHPDAVVIAAAAALEIFHAAALVHDDLIDNSDTRR
                     GGPSAHRHLEAMHRSSGFGGDAARFGRAAALLTGDLLLALSDELFDEAVAACPRPIAA
                     AAKREFSRMRLEVTAGQYLDVLEEDAWVVRDDDDQLARAWTIAVYKSAKYSIEEPLVI
                     GALLGGAGEPQIEALRRYGRPLGVAYQLRDDLLGVYGDPARTGKPAGDDLREGKRTVL
                     VALARRAMTPAKRDRLDSVLGNPKASDEDIRAMQDAIVTCGAVRELERRVESHLARAS
                     DALEGAPFDEASVGRLREIARSAVHRDA"
     gene            5644..6024
                     /locus_tag="VFQ96_03845"
     CDS             5644..6024
                     /locus_tag="VFQ96_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEU0256953.1"
                     /translation="MPLSDHEQRLLDEMERNLYRGDGRFAANMSGKGKRPAYRSVVLG
                     VLAIALGVAVLVTGVFLQVTIVGVIGFAIMFGGVMLTIAPGRGRSGPAGEAGGHRPPP
                     RATTPGFMNRMNDRWDRRQHGDGL"
     gene            6243..6674
                     /gene="mraZ"
                     /locus_tag="VFQ96_03850"
     CDS             6243..6674
                     /gene="mraZ"
                     /locus_tag="VFQ96_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU0256954.1"
                     /translation="MFLGTYTPRLDEKGRIILPAKFREELSEGIVMTRGQEHCIYVFG
                     AREFEALHDRIRQAPVTSKQARDYLRVFLSGASAETPDKQHRVTVPGPLRNYAGLDRD
                     LAVIGAGSRVEIWDAVAWQTYLAAQEAAFAETAEEVIPGLF"
     gene            6774..7727
                     /gene="rsmH"
                     /locus_tag="VFQ96_03855"
     CDS             6774..7727
                     /gene="rsmH"
                     /locus_tag="VFQ96_03855"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU0256955.1"
                     /translation="MTSTPLHIPVLLERSVELLAPALESEGALLVDATVGMGGHAKEI
                     LARLPNVRLIGIDRDEEALEVASRTLAPYQARVTLRHAVYDEIGEVLDDAGESAAAAI
                     LFDLGVSSLQLDRTERGFAYAKDAPLDMRMDTGESRTAADILAEYPEERLSRIFRDYG
                     EERLATRYARRIVAEREARPILTSARLVAIIEEATPAAVRRRGHPAKRVFQALRIAVN
                     DELAALENALPQALARTALGGRVAVMSYQSLEDRIVKRDFAAATASRAPLGLPVEAPA
                     YRPRFVNVFHGAEQADATEIESNPRAASVRLRVVERVRSEA"
     gene            7724..8293
                     /locus_tag="VFQ96_03860"
     CDS             7724..8293
                     /locus_tag="VFQ96_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256956.1"
                     /translation="MSTPRALTSRSSTWHAPAAPERRTDLTIVPGVTRAKRPRIFYAA
                     AAVTAILGVIAAQLLVSLMVTRGAYTVQALQNTQTHVQREYQAASEALARDSSPQNLA
                     AKAHALGMVSTSNVAYLRLSDGKVLGTPKAAKKVAANSVAQKSLVPDALLPGLDQKTP
                     KNARAAGAAGAATTATTPFTGTLASPVTR"
     gene            8349..>9600
                     /locus_tag="VFQ96_03865"
     CDS             8349..>9600
                     /locus_tag="VFQ96_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0256957.1"
                     /translation="MRTTAAIRRRILSIAILAAAIVALFVLRLVNVQVVQANQLTKAA
                     TSDRSVTTTINGLRGPIVDSTGTVLARTVLRWNVQISPSVAITDHDLDKHADEIGKVT
                     GLGGGAVLSIIHQALAQDPKSQYAKIVDGIDATKLDTLTAFHIPWLYFKRVQSRIYPD
                     GQVAGNIVGFVGAQGEAQAGMEYAYNSCLAGTNGTQVYQRGADNVPIPGTTRTTKKAK
                     NGGTLVLTLDADLQWFAQQKLAENVKELGGNYGIVTVMNAKTGQLKAVAEYPSVDPND
                     PGATPAADRGSRAFQAPFEPGSVFKAITASALINEGAATPLTRVLAPYHFTSPGGASL
                     YDAYYHAPWPLTLTGVLVNSSNTGISTLGQRLDDKTRYDYMRKFGVGQAPAIKFPGES
                     AGILNPYQTWGAQTKFATMFGQGVS"
BASE COUNT         1586 a         3412 c         3231 g         1371 t
ORIGIN      
        1 ggcggggcct gaaggggtgg gcgacgagac agttcatgct cgcgacgggg cagatcccca
       61 tcgatcgctc cggcggcaag gcgtctgagg ccgccctcaa cacgggcatg cgggtgctgg
      121 ccgccggcca ggttctcggc atctatccgg aaggcacaag gagccccgac gggcgcctgt
      181 accgcggtcg caccgggatt gcccggatga ttctggaggc gaaggtgccg gtcgttccgg
      241 tcgtgatggt cggtacccgc gagatgatgc cgatcggggc tcgcctgccg aagatccggc
      301 gggtgggcat ggtcgtcggc gagccgctcg atttctcccg tttcgagggg atggagaacg
      361 accggttcgt tctgcgctcg ataaccgacg agatcatgta tgccttgcgc acgttgagcg
      421 ggcaagaata cgtcgacctt tacgcctcga ccgtcaaaga gaggcgtgcc gctctttcac
      481 gataagcttg agcaccagaa tcctggccgt accgggtaca tcccctccgg tacggcttct
      541 cctccggcgc cgtcgtcagg cggtgagatg tgaaatgagc gtgatcggtg caagccacag
      601 agcccgtcgt tgcccccgac aaggacgtca tcgagggtct cgactattgg cggagcctgc
      661 cgatcaaaca gcagccgccc tggcccgacg aggccgcggt gcacgccgcc tcggcggagc
      721 tggccaccct gccgccgctc gtcttcgccg gcgaggtgga ccagttgcga cagcgcctgg
      781 cgaaggccgc gcagggtgag gcgttcctct tacaaggcgg cgattgcgct gagaccttcg
      841 ccggcgccac cgcagaccag atccgcgacc gcgtgaagac ggtgctgcag atggccgtcg
      901 tgctcactta cggcgcgtca gtgccggtca tcaagctggg gcgcatggcc gggcagttcg
      961 cgaagccgcg ctcgcgcgac acggagaccc gcggcggcgt cacgctgccc gcctaccgcg
     1021 gcgacatcgt gaacggctac gacttcaccc cggagtcccg acgcgccgac ccctcccgca
     1081 tgctcgccgg ctaccacacc gccgcatcga ccttgaacct gatccgggcg ttcacgcagg
     1141 gtggcttcgc cgacctgcgc gaggtgcaca gctggaatcg gggcttcgcc tcgaaccctg
     1201 caaaccggcg ctacgaaggc atggcgcacg agatcgaccg tgccatcaag ttcatgcagg
     1261 cggcgggcgc cgacttcgac gagctgaaac gcgtcgagtt ctactccagc cacgaggcgc
     1321 tcttgatgga ctacgagcga ccgctgacgc gcatcgactc gcgcaccggc ctgccgtacg
     1381 acacctccgc gcacctcgtc tgggtgggcg agcgcacccg cgacccagag ggggcgcacg
     1441 tcgacttcct ctcccgggtg cgcaatccga tcggcgtgaa gctcgggccg tcgacgaccg
     1501 gcgacgccat gatgcgcctc gtcgacaagc tcgacccgga acgggagccg ggccggctga
     1561 cgttcatcac ccggatgggg gccggggtca tccgcgacgc gttgccgccg cttctcgaac
     1621 gggcgaagag tgagggcata acgccgctct gggtcacgga cccgatgcac ggcaacggca
     1681 tgacgacgcc ggccggctac aagactcgcc gcttcgacga gatcatcgac gagttgcggg
     1741 gctttttcgc ggtccaccgc gaggtgggca cgaacccggg cggaatccac gtcgagctca
     1801 ccggcgacga cgtgaccgaa tgtctcggcg gctcggaaca catcgacgag gagtccctcg
     1861 agacccgcta cgagtcgctc tgcgaccccc gcctgaacca catgcagtcg ctggaactcg
     1921 ccttcctcgt cgccgaggag ttgcgggcaa cgcagtactg agtctccggc gggcgcccgg
     1981 tggacaccgg gcaggggcca ggtgaggcgg cgagcgcgct caatattggg cgttgatcgt
     2041 gaccttcgca cccttgtcga cgggggtgcc cgccggcggg tcggtcgaca ccgggtcaat
     2101 caccgaccac aggggcttcg gcacgttcgt cttcacgttc acgtggaagc cggcctgggc
     2161 gagagtcttc tcggcgtctt tgatcgacat gcccgtcaca tcgggcaccg gaacctgctg
     2221 cggccccttc gagatcgtga ggatgatcgc atcgcccttg tgcaacggcg ggttgctcgc
     2281 cggagccgcc gagatcacgt ggtctttggg aacagtgtcg ctgtagctgt tcgactccgg
     2341 cccaacctcg aggccggcgg cgttcaaagc gccggaggcg gcggattcgc tctcgcccac
     2401 gacgtcaggc acggggccga gcgagacgac caatgccacc ttttcgcctt cgtgtgccgc
     2461 gccgccggcc gagacgtctc gggcgcccat cctcgcctgg agaaccgttc ccgcctgggt
     2521 cttgtcgaag acctcgtccc ggtccggcaa gaccgtcacg ttgagggctg cgagcgcctt
     2581 cgtcgcctgc gccgccgtca tgccggcgag cttcggcagc gtgaccggcg cggggccgga
     2641 agaaacgcgc agcacgacct tgctgccgcg ccgcacgtcg gtgcccgccc gggggctggt
     2701 cgcgatgacc gtcccgcgcg tcacgcgcag cgaatgctcc gttctctggc cgacgtcgag
     2761 gccgagcctc gtcaaagccg cggtcgccct ggtgggggag gcccccgcga gtcccggcag
     2821 cgagacgaac gcccccggcc cggagccgaa gtaccagcct gcgccgcccg acgcggcggc
     2881 caacaccacg acgaggacga acacccacca gccgcgcagc ttgcggcgtg acgcgcgctt
     2941 ggccaacacc gcgcccgggc cgaagacgcc ggaggcccgg ttaccgctcg accccgggat
     3001 ggcagggcgc ccacgcagcc ggccgggggc ggcgatcggc gcggtgagca ccgcggtcgc
     3061 cccggcgtcg ggcaagacga gcgtggcctg cggcgagacc tcgcccgatg tggtactgcc
     3121 aatgtcggag agctggtcga gcagcacttg ggcgtcggcc ggccgggcat ccggcgactt
     3181 cgccgtcgcc cagaggacga gttcgtcgag ttccggcgag accgccgggt tcgccaggct
     3241 cggcggcggc acggagtcgt tcgcgtgctg gtaggcgatc tgcaccggtt cctcgccgtg
     3301 gaacggctgc tcgccggtga gcatctcgta cagcatgatg cccatcgcgt agatgtcgct
     3361 gcgggtgtcg gcgacgccgc gcgtgacgag ctcgggagag aggtaggcga tcgtgccgag
     3421 gagaagctgg ccgctggccg tcgcggcact ggcggcgcgc gcgaggccga agtcgccgat
     3481 cttgatgcgc ccgtcgtcgc agaggagcac attctccggc ttgaggtctc gatggatgat
     3541 gcctgcccgg tgcgccgccg cgaggccgga gagcacggcc acaccgatcc cgaccgcctg
     3601 cgcaccggtc agcctgcccc gctcacgcag caggtctcgc agcgtgattc ccggcaggta
     3661 ctccatcacg aggtaggcca tgtcgtcgtc ttggccctgg tcgaacacgt tcacgacatt
     3721 gggatgcgcc agacgggcgg cgctgcgcgc ctcttgcacg aaacggtcct tcagtgccgc
     3781 atcgtcggcg agaaagctgt gcatcacctt gatcgccacc tggcgttcca gccgcaagtc
     3841 ggtggccaag tagacggtcg ccatgccgcc gcgggcgatc cgtgaacgca cccggtaccg
     3901 gccgtcgacg agacggccga tcatcgggtc gctcgccgcg gtggtcaccc cagaagtcta
     3961 gggtgtgagc gcacggctcc cgggcgagcc ccggcccgcc gcgaaccggg aagcgaggcc
     4021 ccaggctgct actgtgacct tcgtgcagga ctcatggctc acgctggacg atgccgcccg
     4081 catgctctcg gtcggcgtcg gccgggtgcg acggctcatc gaggaacacc gcctcgcggc
     4141 gatccgggtg caggggaagg cgcgcatacc gcagggtttt gtgcgcgacg gcaagccgct
     4201 gccggagctg cgcggaacgc ttatcgcgct ctccgacgca gggttcgaca acgacgaggc
     4261 ggtcgactgg ctccttcgga agaacgacga cctcgacgcg acacccctcg aggcgctgcg
     4321 cgacggccgc agcaagatgg tgcgctggaa catccagacc ctgggctgag ccccgaccgc
     4381 cgaatctcgc cgcacgtcgg cagcgagtcg cggacggccg gagcagcgcg ccgcccgaca
     4441 cgaccgccgg acgccccgtg atgaaggccg cggactctca ggcgtcgcgg tgcacggccg
     4501 accgggcgat ctcccgcagc cggccgacgc tcgcctcgtc gaacggcgca ccctccaggg
     4561 catccgacgc gcgggcgagg tgcgactcca cccgccgctc caactcccga acggcgccac
     4621 aggtcacgat ggcgtcctgc atcgcgcgaa tatcctcgtc ggaggccttc ggattgccca
     4681 gaacgctgtc cagccgatcg cgcttggccg gggtcatcgc ccggcgggcc agagccacga
     4741 gcaccgtgcg cttgccctcc ctcaggtcat cgcccgcggg cttgcccgtg cgggccgggt
     4801 cgccgtagac ccccagaagg tcgtccctca gctggtaggc gacgccgagc gggcggccgt
     4861 agcgccgcaa tgcctcgatc tgcggctcac cggccccgcc gaggagcgcg ccgatgacga
     4921 gcggttcctc gatgctatat ttcgccgact tgtagacggc gatcgtccac gcgcgcgcga
     4981 gctgatcgtc gtcgtcgcgc acgacccagg cgtcttcttc caaaacgtcg agatactggc
     5041 ccgccgtcac ctccagacgc atgcgggaga actctcgttt cgcggcggcg gcgatcgggc
     5101 gcggacaggc cgcgacggcc tcgtcgaaca gctcatcgct caaggcgaga agcaggtcgc
     5161 cggtcaggag ggcagcggcg cgcccgaagc gtgccgcgtc accgccgaaa cccgacgagc
     5221 ggtgcatcgc ctccagatga cggtgggcgc tcggcccgcc cctgcgggtg tcggagttgt
     5281 cgatgaggtc gtcgtgcacg agcgccgcag catgaaagat ctctaacgcg gcggcggcgg
     5341 cgatgacgac ggcgtcgggg tgcccatcgc cgtcaccctt tcccgacgcc catccgagat
     5401 ggcagaagcg ggcacggaag cgtttgcctc cgccgagaaa ctgccgggag aattccgtca
     5461 ccgcggccag atcgggcgcc acaccgagca gaatgggagt acgggcggcc agatgggcga
     5521 cgacgcggtc ccggacaaga tccgtgagtg tcgtgctttg cgccacctgc ctagcctagc
     5581 cagaaaccgc aagctagaat taggcaagaa tggcattgaa cccgatatga gggggagata
     5641 gagatgccgc tatcggatca cgagcagcgc ctcctcgacg agatggagcg caacctatac
     5701 cgaggtgacg gccgattcgc cgccaacatg agcggcaaag ggaaacgtcc ggcctaccgt
     5761 tccgtcgtgc tcggagtcct cgccatcgcg ctgggggtcg ctgtgctcgt cacaggcgtg
     5821 ttcctccagg tgaccatcgt cggtgtcata ggcttcgcca tcatgttcgg cggggtcatg
     5881 ctgacgatcg cccccggacg aggccgttcc ggcccggccg gcgaggccgg cggtcatcgg
     5941 cctccgccgc gcgccaccac gccgggcttc atgaaccgca tgaacgaccg gtgggaccgc
     6001 cgccagcacg gcgacggcct ctgacagacc caccacaaat ggcctgaccg ggttccggca
     6061 ccaaggcctc acgcaagggc cgcagcacac gctgcggccc tatttttctg tctcccgccg
     6121 gggcgccgtc ggacgcgcca caccgaacat cggaaacgac gccgaaatca tcgaggaggt
     6181 ggatgagagt ggagtagtgt ggggcaatcg tacggcggct cggacggacc aggggggtgg
     6241 cgatgttcct cggcacctac accccccggc tcgatgagaa aggccgcatc atcctgcccg
     6301 ccaagttccg cgaggagctc tcggagggca tcgtgatgac gcgcggccag gaacactgca
     6361 tctacgtgtt cggcgcgcgc gagttcgagg ctctgcatga ccggatccgt caggcccccg
     6421 tcaccagcaa gcaggcccgc gactacctgc gcgtgttcct ctccggcgcc agcgccgaga
     6481 cgccggacaa gcagcaccgg gtcaccgtgc cggggccgct gcgcaattat gcggggctcg
     6541 accgagacct ggcggtcatc ggcgcgggca gccgcgtgga gatctgggac gctgtcgcct
     6601 ggcagaccta cctcgcggcc caggaggccg ccttcgccga gaccgcggag gaggtgatcc
     6661 ccggcctctt ctagcccctg gcccccgact cccagccacg gccgccctga cgccacttcc
     6721 ccggcgccag gtcacctgga tggggatccg ggggcaggga ccggcccggc gccatgacct
     6781 ccactcccct ccacatccct gtcttgctgg aacgcagcgt cgagcttctc gcgcccgcgc
     6841 tcgagagcga gggcgccctc ctggtcgacg ccacggtggg catgggcggg cacgcgaagg
     6901 agatcctcgc gcgcctgccc aacgtgcgtc tcatcgggat cgatcgcgac gaggaggcat
     6961 tggaggtcgc atcgagaacg ctcgcgccgt atcaggcgcg cgtcacgctg cgacacgccg
     7021 tctacgacga gatcggcgag gttctggacg acgcgggcga gagcgcggcc gcggcgatcc
     7081 tcttcgacct cggggtgtcc tccctgcagc tcgaccgcac ggaacgcggc ttcgcctatg
     7141 ccaaggacgc tccgctcgac atgcggatgg acaccggcga gagccgcacc gcggccgaca
     7201 tcctggccga gtaccccgaa gagcggctga gccggatctt tcgggactac ggggaggaga
     7261 ggctcgcgac gcgctacgcc cgtcggatcg tggcggaacg agaagcgcgg cccatcctca
     7321 cctctgcgag gctcgtcgcg atcatcgaag aggccacacc ggccgccgtg cgccgccgcg
     7381 gccacccggc caaaagggtc ttccaggcgc tgcgcatcgc ggtcaacgac gaactggccg
     7441 cgctggagaa cgcactgccg caggccttgg cacgcacggc gctgggcggg cgcgtcgcgg
     7501 tcatgtcgta ccagtcgctg gaagaccgca tcgtgaaacg cgacttcgcc gcggccacgg
     7561 cgtcacgggc acccctcggc ctgcccgtcg aggcccccgc ataccggccg cgcttcgtca
     7621 acgtcttcca cggcgcagaa caggcagacg ccacggagat cgaatccaac ccccgggccg
     7681 cctcggtgcg gttgagggtc gtggaacgag taaggagcga ggcatgagca caccccgtgc
     7741 gttgaccagc cggtcatcga cctggcacgc cccggcggcg ccggagcgcc gcaccgacct
     7801 caccatcgtg cccggcgtga cccgcgcgaa acgcccccgg atcttctacg ccgctgccgc
     7861 cgtcaccgcg atcctcggcg tcatcgccgc ccaactgctc gtgagcctca tggtcacccg
     7921 cggcgcctac acggtgcagg cgctgcagaa cacgcagaca cacgtgcaac gggagtatca
     7981 ggccgcgtcg gaggcgctgg cccgcgactc ctcgccgcag aacctggccg ccaaggccca
     8041 cgcgctcggc atggtctcca cgtccaacgt ggcatacctt cgcctgtcgg acggcaaggt
     8101 gctcggcacg ccgaaggcag ccaaaaaggt cgccgccaat tcggtggcgc agaaatccct
     8161 cgtgcccgac gccctcctgc cgggcctcga ccagaagacg ccgaagaacg ctcgggcggc
     8221 cggagcggcc ggggcagcca ccacggcgac gacccccttc accggcacgt tggcctctcc
     8281 ggtgacccga tgagcggcac cctgacggcg gcccccggcc gcggcaacaa gcccgccgag
     8341 gccctgcagt gaggacgacg gccgcgatcc gccgccggat cctctccatc gcgatcctgg
     8401 ccgcggcgat cgtcgccctc ttcgtcttac ggctcgtcaa cgtgcaggtc gtgcaggcga
     8461 accagttgac gaaggcggcc acgagcgacc gctcggtgac gacgacgatc aacggcctgc
     8521 gcggccccat cgtcgacagc accggcaccg tcctcgcccg cacggtgctg cgctggaacg
     8581 tgcagatctc gccctccgtc gccatcaccg accacgacct cgacaagcac gcggacgaga
     8641 tcggcaaggt cacgggcctg gggggtgggg ccgtgctctc gatcatccac caggcgctcg
     8701 cccaggaccc gaaatcgcag tacgcgaaga tcgtcgacgg catcgacgcg acgaaactcg
     8761 acaccctgac cgcgttccac atcccgtggc tttacttcaa acgggtgcag tcgcgcatct
     8821 acccggacgg ccaggtcgcc ggcaacatcg tcggcttcgt cggggcgcaa ggcgaggccc
     8881 aggccggcat ggagtacgcc tacaactcgt gcctggccgg cacgaacggc acccaggtgt
     8941 accagcgcgg cgccgacaac gtgccgatcc ccggcaccac ccgaaccacg aagaaggcga
     9001 agaacggcgg caccctcgtg ctgacgctcg acgccgacct gcaatggttc gcccagcaga
     9061 agctcgctga gaacgtcaag gagctgggcg gcaattacgg catcgtgacc gtcatgaacg
     9121 cgaagacggg ccagttgaag gccgtcgccg aatacccgag cgtcgacccg aacgacccgg
     9181 gcgcgacccc tgccgccgac cgcgggtccc gcgccttcca ggcaccgttc gaacccggct
     9241 ccgtcttcaa ggcgatcacg gcctcggcgc tcatcaacga gggtgctgcg acgccgctca
     9301 cccgggtctt ggcgccgtac catttcacct ccccgggcgg cgcctcgctc tacgacgcgt
     9361 actaccacgc gccgtggccg ctgactctga ccggcgtgct cgtcaactcg tcgaacacgg
     9421 gcatctcgac gctcggccaa cggctcgacg acaagacgcg ctacgactac atgcgcaagt
     9481 tcggcgtcgg ccaggcgccg gcgatcaaat tccccggcga gagcgcgggc attctgaatc
     9541 cctaccagac ctggggcgcg cagaccaagt tcgccaccat gttcggccag ggcgtctcgg
//
LOCUS       DASTNL010000122         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_644040, whole genome shotgun sequence.
ACCESSION   DASTNL010000122 DASTNL010000000
VERSION     DASTNL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644040"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFQ96_03870"
     CDS             <1..925
                     /locus_tag="VFQ96_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0256958.1"
                     /translation="LYNGQHPQTTEVLVEAFEKATGIRVKVKNGDNQQLANEVIAEGS
                     SSPADVLYTEDSPVLTTVQSKGLLAKVDPGTLAQVPTADSSAKDDWVGVTARAVTLVY
                     DPALISGGGLPTSIMDLAKPAFRGKIGIEQTSGSFEELVTAVRLMKGVSATKAWLAGL
                     KRNAKIYNSHDAILQAVDAGRLPIGLINNYYWFQVRATTKSHTLPSRLLYLGHEDPGA
                     LVSISGAAVLKSAPHAAAAQKFLKYITSPAGQRVIARDQSFEYPLNPEVSPNRQLKPF
                     GQIDPPTLSRLKLGEGQAQSVAMLQQAGFAV"
     gene            922..2529
                     /locus_tag="VFQ96_03875"
     CDS             922..2529
                     /locus_tag="VFQ96_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0256959.1"
                     /translation="MTRREAPARPGPRAQPVPEGRKTAGPGRRRARGPVSLTVLACLV
                     GLLALSPLGYVVGQAAGAGWLPTWRLVTKPATLALLGHTFSLTLAVVAAGIVLGGGGA
                     WLLERTKLPARRFWAVLLPLPIAVPAFVSAFAWVSVLPAMSGFLGAFTVMTLATYPLV
                     LLPTAAVLRDMDPALEEVAASLGQGPWRIFWRTVLPQAMPALAGGALLVALETLAEFG
                     AFSMLNYSTFTTTIYDQYNLTFDGPVASMLAMVLVFACLVLLIAELRVRGRRRYAHVG
                     GGVDRRQSRRSLGRTTPLVMTGLGTLTVAALGVPAGIIAYWITYGRTGRLPLDSLGAA
                     VATSLGLSLAAALTTTAAALPVALLVVRHRSALALLIDRATYLAHGLPGVVVALALVV
                     LSIRYLRPLYQTPALLVIAYVIVFLPLAVVGVRASMARVSAVLPEAARSLGVGPFRAF
                     LRVVLPLIGPGAGAAAALVFLGTMNELTVTLMLAPPGLQTLSTVIWTDTTNLDYPAAA
                     PYAAVMIAVCALPTYLLTNRLGRPAAA"
BASE COUNT          340 a          945 c          858 g          450 t
ORIGIN      
        1 cctttacaac gggcagcatc cgcagacgac cgaggttctt gtcgaggcgt tcgagaaggc
       61 caccggcatt cgcgtgaagg tcaagaacgg cgacaaccaa cagctggcca acgaggtcat
      121 cgccgagggc tcctcgtcgc cggccgacgt gctctacacc gaggattccc cggtgctgac
      181 cacggtgcag tccaaggggc ttctcgcgaa ggtcgacccg ggcaccctcg cccaggtgcc
      241 caccgccgac agttcggcca aagacgactg ggtgggcgtg acggcccgcg cggtgacgct
      301 cgtctacgac ccggccctca tctccggtgg cggcctgccg acctcgatca tggacctcgc
      361 caaaccggcg ttccgcggca aaatcggcat cgagcagacc agcggaagtt tcgaagagct
      421 cgtcacggcg gtgcgtctca tgaaaggcgt ctcggccacc aaagcgtggc tggccgggtt
      481 gaagaggaac gcgaagatct acaacagcca cgacgcgatc ttgcaggccg tcgacgcggg
      541 tcggctgccg atcggcctca tcaacaacta ctactggttc caggtgcggg cgacgacgaa
      601 gtcccacacg cttccctccc gcctgctcta cctcgggcac gaagatccgg gcgcgctcgt
      661 cagcatctcc ggagccgccg tgctgaagtc ggcgccgcac gccgccgccg cgcagaagtt
      721 tctgaaatac atcacgagcc cggccggcca gcgggtcatc gcccgcgacc agagcttcga
      781 atatccgctg aatccggagg tctccccgaa ccgacagttg aaaccgttcg gccagatcga
      841 cccgcccaca ctcagccgcc tgaagctcgg cgaaggccag gcgcagagcg tcgccatgct
      901 gcagcaggcc gggttcgcgg tgtgactagg cgggaggcgc cggccaggcc cggcccccgg
      961 gcgcagccgg tgccggaggg tcgaaagacg gccggccccg ggcggcgccg ggcgcgcggt
     1021 ccggtctccc tcaccgtgct ggcctgcctc gtcgggcttc tcgccctctc gccgctcggc
     1081 tacgtcgtgg ggcaggcggc cggtgccggc tggctgccga cctggcggct cgtcaccaag
     1141 cctgcaaccc tggcccttct cgggcacacg ttctcgttga cgctcgccgt cgtcgcggcc
     1201 ggcatcgtgc tgggcggcgg tggggcgtgg ctcctggagc gcacgaagct tcccgcacgc
     1261 aggttctggg cggttctcct gccgctgccg atcgcggtgc ctgccttcgt ctcggcgttc
     1321 gcctgggttt cggtgctgcc ggcgatgtcc ggctttctcg gcgcgttcac cgtcatgacg
     1381 ctcgcgacgt atccgctcgt gctgctgccg acggccgcgg tgttgcgcga catggacccg
     1441 gcgctggagg aggtggccgc ctcgctcggc caagggcctt ggcggatctt ctggcgcacg
     1501 gtgctgccgc aggcgatgcc ggctctcgcg ggcggtgccc tcctcgtcgc gctcgagacg
     1561 ctcgccgagt tcggcgcatt ctccatgctc aactattcga cgttcaccac gaccatctac
     1621 gaccagtaca acctgacgtt cgacggcccg gtcgcctcca tgctcgccat ggttctcgtc
     1681 ttcgcctgcc tcgtgttgct catcgccgag ctgcgggtgc gcggccggcg ccgctacgcg
     1741 cacgtcggtg gcggggtcga caggcgccag agtcgccgga gtctcggccg gacgacgccc
     1801 ctcgtcatga cggggctcgg caccctcacc gtcgccgccc tcggcgtgcc ggccggcatc
     1861 atcgcgtact ggataaccta tggccgcacg gggcggctgc ccctcgactc gctcggtgcc
     1921 gcggtcgcaa cctcgctggg cctgtcgctg gcggccgcgc tcactaccac ggcggcggcg
     1981 ctgccggtcg cccttctcgt cgtgcgtcat cgcagcgcgc tcgcgctcct catcgatcgg
     2041 gcgacctact tggcacacgg cctgccgggt gtcgtcgtgg cgctcgccct cgtcgtgttg
     2101 agcatccgct atcttcggcc cctttaccag acgcccgccc tcctcgtcat cgcttacgtc
     2161 atcgtgttct tgccgctggc ggtggtcggc gtgcgcgcga gcatggcccg ggtctcggcc
     2221 gtgctgcccg aggcggcacg atcactgggt gtcgggccgt ttcgcgcctt tctccgggtc
     2281 gtcctgccgc tcatcggccc gggcgccggg gccgccgccg cgctcgtctt tctcggaacc
     2341 atgaacgagt tgaccgtcac cttgatgctc gccccgccgg ggctgcagac tctctcgacc
     2401 gtgatctgga cggacacgac gaatctcgac tatccggcgg ccgcgccgta cgcagccgtc
     2461 atgatcgccg tgtgcgcctt gccgacctac ctcttgacca atcgtctggg ccggccggcc
     2521 gcggcgtgag ccgtctggtc gggctcgcgg cacccggtcg gggtgccggg attgtgcgga
     2581 tgactcccag aaa
//
LOCUS       DASTNL010000123        19875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1013332, whole genome shotgun sequence.
ACCESSION   DASTNL010000123 DASTNL010000000
VERSION     DASTNL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013332"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFQ96_03880"
     CDS             complement(<1..294)
                     /locus_tag="VFQ96_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU0256960.1"
                     /translation="MSLGYLVGLDVGTTGAKALVSTAGGQTLAQGHARLDWQATRWGA
                     ETAAQAIFDAALEAVRRALRQAPAGPVAAIGIASFAESSVLLGDTGEPVGPVIA"
     gene            complement(394..1617)
                     /locus_tag="VFQ96_03885"
     CDS             complement(394..1617)
                     /locus_tag="VFQ96_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256961.1"
                     /translation="MAERNIGTVIRFEVVRTLSKRRFWVATLFVPVVLAIVFGLVAAS
                     NSSTSNAADAQKNAHFSFEYSDASGLIAPAIARAAGGTLQANAAEGVADVKSGKTDAF
                     FRYPADPQRQTIRIAAQDVGVFANGKYDAVAQAVLKQSVEQKIGNPTLSALAATTIRT
                     DTVTYTSGARAGGIASIIPPMLFLVLFYLVILLLGNQMLSATLEEKENRVTEMILTTI
                     EARSLILGKVVSLFAVGLVQMLVFAIPVVIGYVFLRDRLNFPSLNLADLIFDPARMIT
                     GALILIGGFALFTGALVALGAVMPTARDAGPIYGALMILIFIPFYAIALIVSDPHAAI
                     VQIFTFFPLSTPITALLRNAFGNLGVGETVAVIAELYIAATLVLWLAVQLFKHGSIEY
                     TRRVNIRAALGAGRR"
     gene            complement(1625..2536)
                     /locus_tag="VFQ96_03890"
     CDS             complement(1625..2536)
                     /locus_tag="VFQ96_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012622966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0256962.1"
                     /translation="MTSGESIVSVRDFRMDFGRQTVIHDLSFEVHAGETFGFLGSNGS
                     GKTTTIRALLGIYEPTAGTLHIRGRRFRPNSSGRLGYLPEERGLYKKEAVIDTMTYFG
                     KLKGMSQAVARSWSLDFLAKVGLAEKEKVRLDKLSGGQQQKVQLGVTIMNQPDLLILD
                     EPTKGFDPVNRRLLMDIIEEQKRSGATVVMVTHQMEEVERLCDRIVLLKDGRSRAYGT
                     VAEVQEQFGGTTVTVRHDGELPRSEDFEVLSDADGAAELALVPGTDLGRLLASFVRAG
                     VGVSEFTPHRRSLDSIFVEVYGESAGE"
     gene            complement(2566..3069)
                     /locus_tag="VFQ96_03895"
     CDS             complement(2566..3069)
                     /locus_tag="VFQ96_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0256963.1"
                     /translation="MTVVRASRDIGLRPLAAKARHRHLEEEIMAGEKISDSISLDDAR
                     RVIDGAIARAIEIGQPQDVAVVDAGGNLKAHVRMDTANVGSISIAINKAYTAIAFQCQ
                     TKDLTAATRPDGSLFGLSDAHGGRIVIFPGGIPLVRDGHIVGAVGVSTGSVEQDQTVA
                     EAGAAAF"
     gene            complement(3120..4469)
                     /locus_tag="VFQ96_03900"
     CDS             complement(3120..4469)
                     /locus_tag="VFQ96_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0256964.1"
                     /translation="MVFDTTVQALSALAPSSAPAAAEAAVSPAAVDSLWLLVGAGLVL
                     LMTPGVAFFYGGMVRAKSVISMMMMSFGSMAVVGVLWVLYGYGFAFGRPIIPHVFGSA
                     DFGMASLLGPNGTTPNMSGLSFAGFQATFAIIAVALISGAIADRARFGAWLLFVAVWV
                     SLVYFPVAYWVFNLRDGWIASVLHVNDFAGGTAVHIAAGSGALALTLVLGKRIGFGRG
                     MYEPHNVPLTLLGAALLWFGWFGFNAGSEGAVDGTTALVWVNTLVAPAAAILGWLGVE
                     RLKSKRMSSIGAASGAVAGLVAITPACNVLTPLWAIVLGALAGAVCALAIDWKFRFGF
                     DDSLDVVGIHMVGGIIGTLFVGVAGMTHVDGDPSKGFSSLLYGGSFRQLAVQAIAVVV
                     VAVFVFVVTYLIGFAIQKTIGFRLPAGAESAGVDLAVHGEVGYALADIAEAPAGAQP"
     gene            complement(4742..6037)
                     /locus_tag="VFQ96_03905"
     CDS             complement(4742..6037)
                     /locus_tag="VFQ96_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256965.1"
                     /translation="MKFLDLLRSAIGNTFRSKLRTTLTVIAIFIGAFTLTITSAIGTG
                     ISNYIDTQVASIGSTNMLTVTKKADAPQAAGSGPAKYNPAQATDSSGFDRGPAASGAV
                     LSKADLTKIARVKGITSVHPVVQISPKYIEYANHGKYQMTVNIAATFTKADLAAGSQL
                     GDANTNEVLLPTSYLKNLGFGGAHSAVGKQVTIAIDDYQGVEHRVDATVVGVQNTTLL
                     NGGVGLNQNLTDQLSEVQATGRPATLAPGYAVAMAHTAKNPTSTQIDAIKKTLSAEGY
                     TGLTVADQIGAFQTVIGGIIGVLNAFAVIALIAAGFGIINTLLMSVQERTREIGLMKA
                     MGMGGGKVYGLFSMEAVFIGFLGSAIGAAVGIGLGSLISNVLSDTVLSGLPGLHVMQF
                     APASIVTIILVVMAIAFLAGTLPARRAARQNPIDALRYE"
     gene            complement(6034..6741)
                     /locus_tag="VFQ96_03910"
     CDS             complement(6034..6741)
                     /locus_tag="VFQ96_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256966.1"
                     /translation="MTTNPVLTAHALHKSYGAGESRFDALKGVSLTVEEGETVAIVGK
                     SGSGKSTLMHLLALLDTPDEGELFLRGKNARTLSRRETNRLRNRDFGFVFQQFFLTPG
                     ASVVDNVILPLKIAGVGSRERRRRALEALDQLELADKAKNKATDLSGGQKQRVVIARA
                     LVNNPTIIFADEPTGNLDTATGTLVEDILFSLNERQGITLVIVTHDTELAERCGRRIF
                     IKDGLEDPTQARQEVES"
     gene            complement(6947..7783)
                     /locus_tag="VFQ96_03915"
     CDS             complement(6947..7783)
                     /locus_tag="VFQ96_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256967.1"
                     /translation="MTALYRLEDVGFRYRTGGDVLDGLSFRLTPGEHLGIVGESGAGK
                     STLLRLLLGLAPVTRGRLVADGRPLNPRDPAQMRAHRRFVQPVFQDPYSSLDPRQRAG
                     RIVGEPLRSLRLADRRSADARARAALAEVGLGDDAAERYPDQFSGGQRQRIAIARALV
                     ARPRVLLADEPVSALDMVTRGRIIDLLERIGRAPGGGTPGERTSTTTSDVPAARQAER
                     ALTMVIVSHDLSIIARLCSVVLVLYRGRAVEYGPTRSVLDSPAQPYTRKLLSSLPRLP
                     RP"
     gene            complement(7785..8642)
                     /locus_tag="VFQ96_03920"
     CDS             complement(7785..8642)
                     /locus_tag="VFQ96_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0256968.1"
                     /translation="MKAPGANDAVLEVRGLTVQTAQGLTLVDGLDLHVAGGERFAVIG
                     ESGSGKSVTGLAVMGLLPQGLTVRGSIRVCGEEVVGAPERRLRRLRGSRVGIVFQEPL
                     TALDPLTRLGAQLGEALARAASAEGRRLRGAGRRRAVREALEAVQIDDADRLARAYPH
                     EASGGQRQRVAIAMALACKPDVLILDEPTTALDVTVQAEVLGLLRRLVADNGMATLFV
                     SHDLAVVAAMAERALVLRAGRAVETGEVTTLLTAPRKAYTRELVAAARALDDSLGDAG
                     RAPGPEGWS"
     gene            complement(8639..9529)
                     /locus_tag="VFQ96_03925"
     CDS             complement(8639..9529)
                     /locus_tag="VFQ96_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256969.1"
                     /translation="MTSNIEAHQVPAGAPSTEPRRQKGGRRRRWSTTGTVGAVLVGAV
                     VVIGVVSLFYLPYPAAQLGGGRLAGPSAQHLLGTDALGRDLLSQFMVGARIALLAGAG
                     SAALGGTLGIVVGLAAAFWKTWADDGAAAVLDVLIAFPSLLLAMLVVAARGATLGSAI
                     LAIGLAIAAIVARLTRILAKRVLGRQFVLAARTSGTGWFGIIRRHILPNIWPTLAVIV
                     ALQFGLAVLAEASLSYLGLGAPPPNASWGSLLHQAQGTVYVAPLGAIMPGIGLVVLVL
                     GVNLLADGMQDAFDPVGGRR"
     gene            complement(9526..10467)
                     /locus_tag="VFQ96_03930"
     CDS             complement(9526..10467)
                     /locus_tag="VFQ96_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0256970.1"
                     /translation="MLRRLAFLLVSLAAASIVTFLLLRLLPGDPANALVAVGATPAQI
                     QAARHAIGSDQPVLQQFITWAGQMARFDLGTSFTSGVAVGPQIVTRMALTLPLTLISF
                     VVAVLIAAPVGFVAARYRERWFGLAASALSQLGLAVPVFWIGILLVWLFALGLHVLPA
                     GGFPEQSWADPADAARSLVLPVVTIALVMSASIARYVRSASIEVIDSDFLRTFRALGA
                     SFGGAMLRHGLKSVAVPLTSVLGIELATTFLGAVVVENVFALPGLGSMLVNGIAAHDY
                     PTIQGVILVTTLLVLVVGFLADLAQKLLDPRLRGSLL"
     gene            complement(10544..12079)
                     /locus_tag="VFQ96_03935"
     CDS             complement(10544..12079)
                     /locus_tag="VFQ96_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0256971.1"
                     /translation="MHKPGALGAVTLAVAAALALAGCSSAGSTGSPAATSGSTAASGY
                     DANATIQVGSIYAPQNLDNTAGGGQGVTEALNGNVYEALYKLGDDGKVTPLLASAEKH
                     SADGLTYTFTLRHGVVFHSGQPLTAQDVKYSIEKVLAPASQSARKSDLQVIKSVATPN
                     DDTVVITLSKPSISLTYNLSYVWIIQRDAKNLKTTEDGTGPYTVTNWNPGTSLTLTRF
                     DKYWGTPAKNKQVTYTYYKDATAENNALLSGQLDVVTSEQGPDALSQFENNPQYKVND
                     GSSTTKLLLAFNDRVAPFNKTAVRVAITRAINRPALLKSVWGSYGQVTGSMVPPTDPW
                     YQNLSSVYPYDPAKAKKELAAAGYPHGFSFTLVTPNYDPHPQAAAFIKQELSKIGVTV
                     KIEIVTPDQWYTKVYQNHDFVATMQEHVNDRDVVWYGNPDFYWGYNNPKVTSLIDQSE
                     EAPSPAGQTADLKKANQIIVKDAASDWLYLYPQIVVAKSTLKGYPLNGLNSQFYVYDI
                     TKS"
     gene            complement(12663..13694)
                     /locus_tag="VFQ96_03940"
     CDS             complement(12663..13694)
                     /locus_tag="VFQ96_03940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU0256972.1"
                     /translation="MSGTVLGDPITNDTIPGGAVRSASVSVITPTLAQPSRTRLLADV
                     YADLSASDVSWEWVIAVDGDNPPEVPERLRTDPRVRVLTIGRRVGAAAARNLALGLAH
                     GRYITCVDDDDRLPAGSLKSRLAAAHSSGAAWVAALLADEHDGAIEVWDSPIARGTLG
                     PGDVWRAWACPCLPFPIGPTTLLVDAGLLRRVGGWQGLPQAEDFGMALAVTSAAPGEM
                     LDRVVYIYHKHPGQTMRRHDFDDLEPLVRHITFERGRLLAEANRKAVPSAMPSSAVVR
                     HHGAPLAPVTTRLTHQFGVEPGDTLWRERALADQAVDRHRRAVHTVRRQLAGHGPHED
                     AQGGLLQTE"
     gene            complement(13767..14552)
                     /locus_tag="VFQ96_03945"
     CDS             complement(13767..14552)
                     /locus_tag="VFQ96_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256973.1"
                     /translation="MPHEPLHPTHGRHAAVPVSPRRTPHLHRAGPARPARARPVPLAP
                     TRQGASPAGAHRHVLTRSTPAWGIVTLSLALTAALSLPSTAAAHAEEITASGAHVPAQ
                     SFLVAGYVHSAQLGHDGLTAEAGNIVITFSTNADWARLVLVDGGWPLTQENVDFMLRW
                     MRQENGPQNWWNRNNPLNNGYGSGGGAGFGSYDNLKDAAHYVAANLQRGYPAVVQALK
                     AGTSADRTAQAIWASPWASSHYAYGAHWSSAPVPIVAAPALAW"
     gene            14639..15577
                     /gene="mvk"
                     /locus_tag="VFQ96_03950"
     CDS             14639..15577
                     /gene="mvk"
                     /locus_tag="VFQ96_03950"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU0256974.1"
                     /translation="MIRLPARAASARAHAKAILIGEHAVVYGGPAIALPVPGLTLTAE
                     VVPADGPARVESALYSGSFENIPPRFAAPATAAAAALELVGAGPTGFRLRISGRIPPA
                     RGLGSSAAVAAAISQAIADAYEAELGEEEHYALVQAAERVAHGTPSGLDARAVLAPGA
                     IWFQAGLSSPLTAGPGGVFVVADTGEAGGTAGAVAAVRRLKAADEARVRGVLGDLDAL
                     TAEAAQSLVAGDTAALGAAMTAAHTLLIRLTVSSPGLDHLVRAAREGGALGAKLTGGG
                     RGGCILALAEGRAAAERLAEALRAAGAAATWILGAP"
     gene            15574..16629
                     /gene="mvaD"
                     /locus_tag="VFQ96_03955"
     CDS             15574..16629
                     /gene="mvaD"
                     /locus_tag="VFQ96_03955"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU0256975.1"
                     /translation="MTPALAEATALAHPNIALIKYWGKEDDDLVLPAAGSLSLTLDVF
                     PTTTTVTPDTGLTRDVVELDGRPAGPTTAYRVGAFLDLVRALAGRAGAKPLHARVRTR
                     NAVPTGAGLASSASGFAALALAASSAYGLRLDRPGLGRLARRGSGSACRSIVPGLALW
                     RAGDDASSYAVPVEGPPLGMVIVVADVGGKKVSSREAMRRTRDTSPYYAAWVRSAAAD
                     LGPALEALRHDDLAALGELVESNALRMHAAIAACRPPIRYLRPESLMVFEAAEELRVS
                     GLGVYATADAGPNVVLVCRLRDLARVSEALVGRLGQAFAGRIVSAGPGPGARLVPVGT
                     AAEDPARGAPPAVRGRP"
     gene            16626..17693
                     /locus_tag="VFQ96_03960"
     CDS             16626..17693
                     /locus_tag="VFQ96_03960"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU0256976.1"
                     /translation="MIRATAPGKLFVAGEYAVVEPAQPAVLVAVDRYITVAVDPSPTT
                     GAGRLVSTVTSPGRAAATWHRAGPGIETAGGTAAFRYLLAAVAIVEELAAERGVPFSG
                     CRMRVTSTLEGAGRKLGLGSSAAVTVAAVRALGEFYGLGLARMAVLKAALAATFRVAP
                     EASGGDVASSVFGGWIVYASPRREALGGMGVAAAELVTREWPGLCVRHLPAPAGLRLL
                     VGWTGNPAVTGRLVADVRGRAGGTPRDFVAQSRRAVLGLVGALQDGDADRVLLEVRRC
                     RALLAGLAGQTGVAIETPLLAALCDAAEGEGAAAKPSGAGGGDCGIALAPPGADAEAI
                     LQEWIRRGIRPLDLRPAPNID"
     gene            17749..18879
                     /gene="fni"
                     /locus_tag="VFQ96_03965"
     CDS             17749..18879
                     /gene="fni"
                     /locus_tag="VFQ96_03965"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU0256977.1"
                     /translation="MSAERKDQHLALADEQQLGGPARNDFDDVRFVHHALAGIDRDGI
                     RSAVSFAGLRWARPLYINGMTGGSARAGRVNRDLAVAARETGVPIASGSMSICFKGEA
                     DEATLDTFRVLRRENPDGFVMANLSADATPDEAARAVDLLAADALQIHLNSVQETVMP
                     EGSRGFSAWPDQIAAIVEAVETPVIVKEVGFGLSRASLERLALLGVGIADVSGRGGTD
                     FARIENARRSGGAAPAGTLGAGDAGFGYGFMAGWGQSAVCSLLDAKAGDEGVPALLAS
                     GGVRTPLDVVRSLALGAHAVGVSGVFLHAVLEGGAAGLVAMIRAWLEQIAKLQALLGA
                     ASPAALTGTDVLLTGAVREYCQLRGIDAASYARRSERPGAGR"
     gene            18876..>19875
                     /locus_tag="VFQ96_03970"
     CDS             18876..>19875
                     /locus_tag="VFQ96_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEU0256978.1"
                     /translation="MSRAGAAAVQGGGAREEREAGRPAETAPIPLSWVGPLLLRGPVA
                     SGEQWVPLATFETPLWPSVRRGAKISTMTGGILTTLVDERMTRSVILEAGDAGEALAA
                     WQRIDAAREELQALVAQVSGHARLLGLNRQIVGNLLFLRLELAVGDAAGHNMVTLAAD
                     RIIDHILTRLPGLRYGSVSGNYCTDKKASAVNGILGRGKNVVAELNVPRRLVERLLHT
                     TPEAISTLNVRKNLIGSTLAGSIRTANAHYANMLLAFFLATGQDAANIVEGSQGITHA
                     ESREGDLYFSCTLPNLVVGTVGNGKDLPFVARNLERLGCRTGSTDGTDARRLAVLCA"
BASE COUNT         2954 a         6810 c         7122 g         2989 t
ORIGIN      
        1 ggcgatgacg gggccgacgg gctcgcccgt atcgcccagg agcacgctgg attctgcgaa
       61 actggcgatg ccgatcgccg cgacggggcc ggcaggggcc tgccggaggg cgcgtcgcac
      121 ggcctccagc gccgcgtcga agatggcttg cgccgcggtc tcggcgcccc acctcgtggc
      181 ttgccagtcg agccgggcgt gcccctgcgc gagcgtctgg ccgcccgccg tcgagacgag
      241 ggctttcgcg ccggtcgtgc ccacgtcgag ccccacgaga tacccgagcg acacgcgcaa
      301 ctccctgaca tcgacgacgt tggaccggcg tggacatcgt cgcatcccgc aggtggaggg
      361 gtcaacttgc cgtggggccc tcggcccttg ccgctaccgc cgcccggcgc cgagagccgc
      421 gcggatgttc acgcggcggg tgtattcgat cgagccgtgt ttgaacagct gcacggccag
      481 ccacagaacg agcgtcgccg cgatgtacag ctcggcgatg acggcgacgg tctcgcccac
      541 gccgagattg ccgaaggcgt tgcgtaagag ggcggtgatc ggcgtggaga gggggaagaa
      601 ggtgaagatc tgcacgatgg ccgcatgggg gtcggagacg atgagggcga tcgcgtagaa
      661 ggggatgaag atcaggatca tgagggcccc gtagatgggc cccgcgtcgc gggcggtggg
      721 catgacggcg cccagggcga ccagcgcgcc ggtgaacagg gcgaacccgc cgatcaagat
      781 cagcgcgccg gtgatcatcc gtgccgggtc gaagatcagg tcggcgaggt tcagcgaggg
      841 aaagttcagc ctgtcgcgca agaagacgta gccgatcacg acgggaatcg cgaagacgag
      901 catctgcacg agtccgaccg cgaacaggct gacgaccttg ccgagaatca ggctgcgggc
      961 ctcgatcgtg gtcaggatca tctcggtgac cctgttctcc ttctcctcga gggtggcgga
     1021 gagcatctgg ttgccgagca gcaggatcac caggtagaag agaacgagga agagcatcgg
     1081 cgggatgatc gaggcgattc cgccggcccg cgcccccgag gtgtaggtga ccgtgtcggt
     1141 gcggatggtc gtcgcggcga gggcgctgag cgtcgggttg ccgatcttct gctccacgct
     1201 ctgtttcagc acggcttggg cgacggcgtc gtatttgccg ttcgcgaaga cgccgacatc
     1261 ttgcgccgcg atacggatgg tctgccgctg cgggtcggcc gggtaacgga agaacgcatc
     1321 ggtcttgccc gacttcacgt cggcgacacc ctcggcggcg tttgcctgca gggtgccgcc
     1381 tgcggcccgc gcgatggcgg gggcgatcaa gccggaggcg tcggagtatt cgaacgagaa
     1441 gtgcgcgttc ttctgtgcat cggccgcgtt cgacgtgctg gagttcgagg ccgcgaccag
     1501 gccgaagacg atcgccagga ccaccggaac gaacagggtc gccacccaga agcgccgctt
     1561 cgaaagggtg cgcacgacct cgaagcgtat gacggtgccg atgttgcgct cagccatcgc
     1621 gcccttactc cccggcgctc tcgccgtaga cctcgacgaa gatcgagtcg agggagcgcc
     1681 ggtgcggcgt gaactctgac acgccgaccc cggcccgcac gaacgaggcc aggagccggc
     1741 cgaggtccgt gcccggcacg agggcgagct ctgccgcccc gtcggcatcc gagagcacct
     1801 cgaagtcttc cgagcggggc agctccccgt cgtgccgcac cgtgaccgtc gtgccgccga
     1861 actgctcctg aacctcggcg acggtgccgt aggcgcggga gcggccgtct ttcaagagca
     1921 cgatgcggtc gcagagccgc tcgacctctt ccatctggtg agtgaccatc accacggtgg
     1981 cgccggagcg cttctgctcc tcgatgatgt ccatgagcag ccggcggttc acggggtcga
     2041 accccttcgt cggctcgtcg aggatcaaga gatccggctg gttcatgatc gtcacgccga
     2101 gttgaacctt ctgctgctgg ccgcccgaga gcttgtccaa ccgcaccttc tccttctcgg
     2161 caaggccgac ctttgccagg aagtcgagcg accacgaccg ggccacggcc tggctcatgc
     2221 ctttgagctt gccgaagtag gtcatcgtgt cgatgacagc ctcttttttg tagaggcctc
     2281 gttcttccgg caggtagccc agccgacccg aactgttcgg ccgaaacctg cggccgcgga
     2341 tgtggagtgt gcccgccgtc ggctcgtaga tgccgaggag cgcccggatg gtcgtcgtct
     2401 tgcccgaccc gttcgagccc aggaaaccga acgtctcccc cgcgtgcacc tcgaacgaca
     2461 ggtcgtggat gaccgtctgc cggccgaagt ccatgcgaaa gtctcgaaca gagacgatcg
     2521 attcccccga agtcatcccg ctactgtagc gcgggccgtg cgcgttcaga acgcggcggc
     2581 accggcctcc gcgaccgtct ggtcctgctc gacggagccc gtcgagacgc cgaccgcgcc
     2641 cacgatgtgg ccgtcacgca ccagggggat gccgccgggg aagatcacga tgcgcccgcc
     2701 gtgcgcgtcg ctcaacccga acaggctccc gtcgggccgc gtcgcggcgg tcaggtcttt
     2761 ggtctggcac tggaacgcga tcgccgtata ggccttgttg atcgcgatgg aaatgctgcc
     2821 gacgttcgcc gtgtccatgc gcacgtgagc cttcaagttg ccgccggcgt cgacgaccgc
     2881 cacgtcttgc ggctgcccga tctcgatcgc ccgcgcgatc gcgccgtcga tgacccgtcg
     2941 tgcgtcatcc agcgagatgc tgtcgctgat cttttctccg gccatgatct cctcctcgag
     3001 atgtcggtgg cgcgctttcg cggccagcgg ccgcagcccg atgtcacgcg aggcgcgcac
     3061 aaccgtcatg ctaggccgcc tcgtcgatgc cgaccagggg aggagagccc gggcggaggt
     3121 cagggctgtg cgcccgcggg agcctcggcg atgtcggcca gggcgtagcc cacctcgccg
     3181 tgcaccgcga ggtcgactcc ggctgactcg gcgcctgccg gcaggcggaa cccgatcgtc
     3241 ttctgaatcg cgaagccgat caggtaggtg acgacgaaca cgaacaccgc gacgacgacg
     3301 acggcgatcg cttgaacggc cagctggcgg aagctgccgc catagagcag gcttgagaaa
     3361 cccttgctcg ggtcgccgtc gacatgtgtc atgccggcca ccccgacgaa gagtgttccg
     3421 atgatgccgc cgaccatgtg gatgccgacg acgtcgagcg agtcgtcgaa accgaagcgg
     3481 aacttccagt cgatcgccaa cgcgcatacg gcgcccgcca gcgcgccgag cacgatcgcc
     3541 cagaggggtg tcagcacgtt gcaggccggg gtgatggcga cgaggcccgc cacggcgccc
     3601 gacgccgccc cgatcgaact catccgcttg ctcttcagcc gctccacccc gagccagccg
     3661 aggatcgcgg ctgcgggcgc caccagcgtg ttcacccaca cgagcgccgt cgtgccgtcc
     3721 accgctccct cggagccggc gttgaagccg aaccagccga accacaggag cgccgcgccg
     3781 agcagggtca acggaacgtt atgcggctca tacatgccgc gaccgaagcc gatgcgcttg
     3841 cccaagacga gcgtcaacgc caacgcgccc gacccggccg cgatgtgcac ggcggtgccg
     3901 ccggcgaagt cgttgacgtg cagcacgctc gcgatccagc cgtctcgcag gttgaagacc
     3961 cagtaggcga ccggaaagta cacgaggctc acccagacgg cgacgaacaa cagccaggcg
     4021 ccgaaccgcg cccggtcggc gatcgcgccg gagatcagcg cgaccgcgat gatcgcgaag
     4081 gtcgcctgga agccggcgaa cgagagcccc gacatgttcg gtgtcgtacc gttcggcccg
     4141 agcaggctcg ccatgccgaa gtcggcgctg ccgaagacgt gcgggatgat cgggcgcccg
     4201 aaggcgaacc cgtagccgta gagcacccac agaactccga ccaccgccat cgagccgaag
     4261 ctcatcatca tcatgctgat gacacttttc gcacggacca tgccgccgta gaagaacgcg
     4321 actcccgggg tcatcaacag cacgaggccc gccccgacga gcagccagag ggagtccacc
     4381 gcggcgggtg agaccgccgc ctcggctgcc gccggcgcag aggatggggc gagcgcggaa
     4441 agcgcctgca ccgtcgtgtc gaacaccatg gtccacctcc tggggcgcgc tcaccggcct
     4501 tcggtgatct tgcctagaag gtgattgtgc ccgggggatg tttcggccga ggcacgcgcg
     4561 tgtttcccgt cgatgacggg atcggcccgg gtgtgaacat cgtgttacgc cggcgtcgcg
     4621 acgacaggca cgaggcgctc gcgtggcctt gtgagagcgt cgggcgagcc acagatgccc
     4681 gcgggatgca actgcggctg ggtcttcggg cgttctttac tcgggccttc gggcgcccta
     4741 cctattcgta gcgcaacgcg tcaatcgggt tctggcgggc cgcccggcgc gcggggaggg
     4801 ttcccgccag gaacgcgatc gccatgacga cgaggatgat cgtgacgatc gaggccggcg
     4861 cgaactgcat gacgtgcagg ccgggcaggc ccgacagcac cgtgtctgaa agcacgttgc
     4921 tgatcagcga gccgagcccg atgccgacgg ccgcgccgat ggcgctgccc aggaagccga
     4981 tgaagaccgc ctccatgctg aaaagcccgt agaccttgcc gccgcccatg cccatcgcct
     5041 tcatgaggcc gatctcacgg gtgcgctcct gcacgctcat caagagtgtg ttgatgatgc
     5101 cgaacccggc cgcgatcagg gcgatcacgg cgaacgcgtt caagacgccg atgatgccgc
     5161 cgatgaccgt ctggaacgcg ccgatctggt cggcgacggt gagcccggta tagccttcgg
     5221 ccgacagggt cttcttgatg gcgtcgatct gcgtgcttgt cgggttcttg gcagtatgcg
     5281 ccatcgccac ggcgtaaccc ggtgcgagcg tggcgggccg acccgtcgcc tgcacctcgc
     5341 tcagctggtc ggtgaggttc tggttcaggc cgacgccgcc gttcaagagg gtcgtgttct
     5401 gcaccccgac cacggtggcg tccacgcgat gctcgacacc ttgatagtcg tcgatcgcga
     5461 tggtgacctg cttgccgacc gcggagtgcg cgcccccgaa accgaggttc ttcagatacg
     5521 acgtgggcag cagcacctcg ttcgtgttgg cgtcgcccag ctggctcccg gcggcaaggt
     5581 cggccttcgt gaacgtggcc gcgatgttca ccgtcatctg gtacttgccg tggttggcgt
     5641 attcgatgta tttcggcgag atctgcacga ccggatgcac gctcgtgatg cctttgacgc
     5701 gggcgatctt cgtgaggtcg gccttgctca gcacggcgcc cgaggcagcg gggccgcggt
     5761 cgaacccgct ggaatcggtc gcctgcgcgg ggttgtactt ggccgggccc gagcccgcgg
     5821 cctgcggcgc gtcagccttc ttcgtgaccg tcagcatgtt ggtcgagccg atcgaggcga
     5881 cctgcgtgtc gatgtagttg gagatgcccg tgccgatggc gcttgtgatc gtgagggtga
     5941 acgcgccgat gaagatcgcg atgacggtga gcgtggtacg cagcttgctg cggaacgtgt
     6001 tgccgatcgc cgagcgtaag aggtcgagga acttcacgat tccacctcct gtcgcgcctg
     6061 cgtcggatct tcgaggccgt ctttgatgaa gatgcgccgg ccgcagcgct ccgccagctc
     6121 ggtgtcgtgc gtgacgatga cgagcgtgat gccctgcctc tcgttcaacg agaacaagat
     6181 gtcttcgacg agcgtcccgg tcgccgtgtc gagattgccg gtgggctcgt cggcgaagat
     6241 gatcgtcggg ttgttcacca gcgcccgggc gatgacgacg cgttgcttct ggccgccgga
     6301 gaggtccgtc gccttgttct tggccttgtc agccaactcc aactggtcga gcgcctccag
     6361 cgcgcgtcgt ctgcgttccc gcgagccgac ccccgcgatc ttcaacggca ggatcacgtt
     6421 gtcgacgacc gacgcgcccg gcgtgaggaa gaactgctga aagacgaaac cgaaatccct
     6481 gttccgcagg cggttggtct ctcgacggga gagcgtgcgg gcattcttgc cccgcaggaa
     6541 caactcgccc tcgtcggggg tgtcgagcag ggcgagcagg tgcatgagcg tcgacttgcc
     6601 cgagccgctc ttgccgacga tcgcgaccgt ctcgccctcc tcaaccgtca aggagacgcc
     6661 cttcagggca tcgaaacggc tctcgccggc gccgtacgac ttatggagcg cgtgtgccgt
     6721 cagaacagga ttcgttgtca ccggtaggtc atctcgtgaa taaggcaggc gcgagaacgg
     6781 gttcgtcgcg ccggcgccgt aggaacgcgg ggcgtgcagg gccatcctaa cggccgcgtt
     6841 ctgcgcgccg gccgagagcc ccctgtcccg gggagggttc tcggcgccgg gtacggtgca
     6901 ggagtctcga ggcatccggg tgtcagggcg ggagacggct gtcgtgtcaa ggtcgcggca
     6961 gccgaggcag gctggaaagg agcttgcggg tgtatggctg ggcgggggag tccaagaccg
     7021 atcgggtggg tccatactcc acggcccgcc cgcggtaaag caccaggacc acgctgcaca
     7081 gccgggcgat gatgctgagg tcgtgtgaga cgatcaccat cgtgagcgcc cgctcggcct
     7141 gtcgcgcggc cggcacatcg ctcgtggtcg tcgatgtgcg ctctcccggg gtgcccccgc
     7201 ccggtgcccg gccgatccgt tccagaaggt cgatgatccg cccgcgcgtc accatgtcga
     7261 gagccgagac cggctcgtcg gcgagcagca cccgggggcg tgcgacgagg gcgcgcgcga
     7321 tggcgatgcg ctggcgttgg ccgccggaga actggtcggg gtagcgctct gccgcgtcgt
     7381 cgccgagccc cacctcggcg agggcggcgc gggcgcgggc gtcggccgag cgccggtcgg
     7441 cgaggcgcaa cgagcgcagc ggctcgccga cgatgcggcc cgcccgttgg cgagggtcca
     7501 acgacgagta cgggtcttgg aagaccggct gcacgaagcg gcggtgcgcc cgcatctggg
     7561 ccgggtcgcg cgggttgagc ggacgcccgt cggcgacgag gcggccccgg gtcacgggcg
     7621 ccaggccgag caacaaacgt aagagcgtcg acttgccggc gccggattcg ccgacgatgc
     7681 cgaggtgttc gcccggcgtc agccggaacg agaggccgtc aagcacgtcg ccgcccgtgc
     7741 ggtagcggaa acccacgtct tccaatcggt ataaggccgt catgtcacga ccacccctca
     7801 gggccgggcg cccggccggc gtcgcccagg ctgtcgtcca gcgcgcgtgc cgccgccacg
     7861 agctcgcgcg tgtaagcctt cctcggtgcc gtgaggagcg tcgtgacctc gccggtctcg
     7921 acggcacggc cggcccgtaa gacgagcgcc cgctcggcca tcgcggcgac cacggcgagg
     7981 tcgtggctga cgaacagcgt cgccatgccg ttgtcggcca cgaggcggcg caagaggccc
     8041 agcacctcgg cctgcaccgt cacgtcgagg gccgtggtcg gctcgtcgag gatcaacacg
     8101 tcgggtttgc aggcgagcgc catcgcgatc gcgacccgct gccgctggcc cccggaggcc
     8161 tcatgcgggt acgcccgcgc caggcgatcg gcgtcatcga tctgcaccgc ctcgagcgcc
     8221 tcccgcaccg cgcgcctgcg ccccgcgccc ctcagccgcc ggccctctgc cgaggcggcc
     8281 cgggccaggg cctcccccag ctgcgcgccg agcctcgtca aggggtcgag ggcggtgagc
     8341 ggctcttgga agacgatgcc cacccgggag ccgcgcagcc gccgcagccg ccgctccggc
     8401 gcgccgacga cctcttcgcc gcagactcgg atgctgcctc ggacggtgag gccctgcggc
     8461 aggaggccca tcaccgcgag cccggtcacc gacttgcccg agcccgactc gccgatcacc
     8521 gcgaagcgct cgccgccggc cacgtgcagg tcgagcccgt cgacgagggt caggccctgc
     8581 gccgtctgca cggtgaggcc ccgcacctcc aacacggcgt cgttcgcgcc aggggccttc
     8641 accgcctgcc gcccaccggg tcgaaggcgt cttgcatgcc gtctgccaag aggttcacgc
     8701 ccaggacgag cacgacgagg ccgatgccgg gcatgatcgc gccgagcggc gccacgtaga
     8761 ccgtgccttg cgcctggtgc aggaggctgc cccacgaggc gttcgggggc ggggcgccga
     8821 ggccgaggta ggacaggctc gcctcggcca agacggccaa cccgaactgc aaggcgacga
     8881 tcacggccag agtaggccag atgttcggca ggatgtggcg tcggatgatg ccgaaccagc
     8941 ccgtccccga cgtgcgggcc gccagcacga attgccggcc gagcacccgt ttggcgagga
     9001 tccgtgtgag ccgggcgacg atggctgcga tcgccaggcc gatcgcgagg atcgcggagc
     9061 cgagcgtggc cccgcgggcg gccacgacga gcatcgccaa gagcagcgac gggaaggcga
     9121 tgagaacgtc gagcaccgcc gcggccccgt cgtcggccca ggtcttccag aacgctgcgg
     9181 cgaggccgac gacgatgccg agagttccgc cgagggcggc ggagcccgcc ccggccagaa
     9241 gggcgatgcg ggcgccgacc atgaactgcg agagcaggtc gcgaccgagc gcgtcggtgc
     9301 cgagcaggtg ctgggcggag ggaccggcca ggcggccgcc gcccagctgc gcggccggat
     9361 acggcaggta gaagagcgag acgacaccga tcacgacgac ggcgccgacg agcacggcgc
     9421 cgacggtgcc cgtcgtcgac catcgtcgcc ggcgcccgcc cttctggcgc cgcggctcgg
     9481 tggacggtgc gccggccgga acctggtggg cctcgatgtt cgacgtcaca gcagagatcc
     9541 tcgcagtcgc gggtcgagca gcttctgcgc gaggtcggcg aggaagccga cgaccaagac
     9601 gagcagggtc gtcacgagga tcacgccttg gatcgtcggg tagtcgtgcg cggcgatgcc
     9661 gttcaccagc atgctgccga ggcccggcag ggcgaagacg ttctcgacga ccaccgcgcc
     9721 gaggaacgtc gtggcgagct ctatgcccag cacggaggtg agcggcacgg ccaccgactt
     9781 caggccgtgc cggagcatcg cgccgccgaa actggcaccc agcgcgcgaa aggtgcgcag
     9841 aaagtcgctg tcgataacct cgatggaggc cgagcgcacg taccgggcga tcgacgccga
     9901 catgacgagg gcgatcgtca cgaccggcaa gacgagggag cgcgccgcgt cggccgggtc
     9961 ggcccaggac tgttcgggaa acccaccggc aggcagcaca tgcagaccga gggcgaagag
    10021 ccagacgagc aggatgccga tccagaagac gggcacggcg aggcccagct gggagagcgc
    10081 cgaggccgcc agcccgaacc agcgctcgcg gtagcgggcc gcgacgaaac cgaccggcgc
    10141 ggcgatgaga acggcaacca cgaaggagat cagggtcaaa ggcagcgtga gggccatccg
    10201 cgtgacgatc tgcggtccca cggccacgcc gctcgtgaac gacgtaccga gatcgaaccg
    10261 cgccatctgg cccgcccagg ttatgaactg ttgcagcacg ggctggtcgg agccgatcgc
    10321 gtgccgtgcc gcctggatct gggccggggt ggcgcccacc gcgaccaggg cgttcgccgg
    10381 gtcccccggc aggagcctca gcagaagaaa cgtcacgatg gaggcggcgg ccagggagac
    10441 gagcagaaat gcgagccggc ggaggatgta tccggccgtc gttcagccct cctccgggcg
    10501 gggatcgacc cgggccgccc gcccgggttc cgtggtgccg cgctcagctc ttcgtgatgt
    10561 cgtagacgta gaactgcgag tttagcccgt tcaacgggta gcccttgagc gtcgacttcg
    10621 cgaccacgat ctgcgggtaa aggtagagcc agtcgctggc tgcatccttc acgatgatct
    10681 ggttcgcctt cttgagatcg gcggtctggc ccgccgggga gggtgcctcc tccgactggt
    10741 cgatcagcga cgtcaccttg gggttgttgt agccccagta gaagtcgggg ttgccgtacc
    10801 agacgacgtc gcggtcgttg acgtgctctt gcatcgtcgc cacgaagtca tggttctgat
    10861 acaccttcgt gtaccactgg tcgggcgtca cgatctcgat cttcaccgtg acgccgatct
    10921 tcgacagctc ttgcttgatg aaagcggccg cttgcgggtg cgggtcgtag ttcggggtga
    10981 caagcgtgaa gctgaagccg tgcgggtagc cggcggcagc cagctccttc ttcgccttcg
    11041 cagggtcgta gggataaacg ctgctgaggt tctgatacca cgggtcggtg ggcggaacca
    11101 tggacccggt cacctgcccg tatgatcccc atacggattt caggagggcc gggcggttga
    11161 tggcccgggt gatcgcgacg cgcaccgcgg tcttgttgaa cggagccacc cggtcgttga
    11221 acgcgaggag cagcttcgtc gtcgacgagc cgtcgttgac cttgtactgc gggttgttct
    11281 cgaactggct gagagcgtca gggccctgct cgcttgtgac gacgtcgagc tgccccgaca
    11341 agagcgcgtt gttctcggcc gtcgcgtcct tgtagtaggt gtaggtcacc tgcttgttct
    11401 tggccggcgt gccccagtac ttgtcgaagc gcgtcagcgt gagggaggtt ccgggattcc
    11461 agttcgtgac cgtgtaaggg ccggtgccgt cttccgtggt cttcaggttc ttggcgtcgc
    11521 gttggatgat ccacacgtag ctcaggttgt aggtgagcga gatggagggc ttcgacagcg
    11581 tgatgacgac cgtatcgtcg ttcggcgtcg cgaccgactt gatgacctgc aggtcgcttt
    11641 tgcgggcaga ctgcgaggcg ggggccagta ccttctcgat gctgtacttc acgtcttggg
    11701 cggtcagcgg ctgcccggaa tggaacacga cgccgtgtcg cagggtgaag gtgtaggtca
    11761 gcccgtcggc ggagtgcttt tcggcggagg ccaggagcgg cgtgactttg ccgtcgtcgc
    11821 ccagcttgta cagcgcctcg tagacgttgc cgttcagcgc ctcggtgacg ccttgcccgc
    11881 cgcccgccgt gttgtcgagg ttctgcggtg cgtagattga gccgacttgg atggtcgcgt
    11941 tcgcgtcgta tcccgaagcg gcggtggagc ccgatgtggc ggcgggcgat cccgtggagc
    12001 cggccgacga gcatccggcc aaagccaatg cggcggcgac ggccaacgtc acggcgccca
    12061 gggcgccagg tttgtgcatc gtgctgttct cctcttgtcg ctcacggggc gccgggccct
    12121 caaagaccat cctctcgtgc gggctttggt gcgggccgct gtgttacgtc gtattgccct
    12181 gttcgggcga accctagggt ggaggcatgg gtacacgttc caaagcagtg ttgatcgtcg
    12241 gagccgggcc gcgtctgggg gcggccatcg cccgcaggtt cgggcgagac gggtacgccg
    12301 ccgccctcgt ctcccgccgg gcggaaggcg tcgcccgcct cggtgaggcg ctctctgccg
    12361 aagggatcga gacgcactgg gcggcggccg acgtcgcccg ccccgccgaa ctgagggccg
    12421 cgatcgatcg cttggccggc gaagtccagg cgatcgacgt tgccgtgcac aacatctcga
    12481 cctggcgtga cgccggggcg ctcgacctca caccggacca gctgcttgcc gatctcgggg
    12541 tcggcaccgc gagcctcttg accatcgccc aggcagtggc gcccgccatg atggaacgcg
    12601 ggggaggaac gatcctcgcc acgggttccg gcgccgcgga caaccccacg ccgggggccc
    12661 cgtcactcgg tctgcagaag gccgccctgc gcgtcctcgt gcgggccatg gccggcgagc
    12721 tggcgcctca cggtgtgcac tgcgcgacgg tgacgatcga cggcctgatc ggcgagggcg
    12781 cgttcgcgcc agagcgtatc gccgggctct actccgaact ggtgcgtgag acgcgtggtg
    12841 accggggcga gtggcgcacc gtggtgccgt acaacggctg acgacggcat cgccgacggc
    12901 accgctttgc ggttcgcctc ggccagaagc cggccgcgtt cgaacgtgat gtgccgcacc
    12961 aacggctcca ggtcgtcgaa gtcgtgccga cgcatcgtct ggccgggatg cttgtgatag
    13021 atgtacacca cccggtcgag catctcgccg ggtgcggcgg aggtcacggc caaggccatg
    13081 ccgaagtctt ccgcctgcgg caggccctgc cagccgccga cacggcgcag cagcccggcg
    13141 tcgacgagga gcgtggtcgg gccgatcggg aacggcaggc acgggcaggc ccacgcgcgc
    13201 cacacgtcac ccgggccgag ggtgccgcgc gcgatcgggc tgtcccacac ctcgatggcg
    13261 ccgtcgtgct cgtcggcgag aagcgccgcc acccaggcgg ctcccgagga gtgggcggcc
    13321 gcgaggcggg acttcagcga gccggccggc agccggtcgt cgtcgtcgac acaggtgatg
    13381 tagcggccgt gcgcgagccc caaggccagg ttgcgggccg cggcggcacc cacccgccga
    13441 ccgatggtga gaactctcac ccgggggtcg gtgcgcagcc tttcgggcac ctccggcggg
    13501 ttgtcgccat ccacggcgat gacccactcc caggagacgt cacttgccga gaggtccgcg
    13561 tagacgtcag cgagcagccg ggtgcgagac ggctgtgcga gggtgggcgt gatcacgctg
    13621 accgacgcag accgcacggc gccgccgggg atcgtgtcgt tggtgatggg gtcgcctaaa
    13681 accgtgccgc tcacccagat cgatgctccc tcgaggcccc cgccacaggc agcttacccg
    13741 cccgcccttc tgccgggtgg gcaggcttac cacgccaacg cgggggcggc gacgatcggc
    13801 acgggggcgc tgctccagtg cgcgccgtat gcgtaatggc tcgaggccca cggcgacgcc
    13861 cagatggcct gggcggtccg gtcggcggag gtgccggcct tcaatgcctg gacgacggcc
    13921 gggtagccgc gctgcaggtt cgcggcgacg taatgcgccg cgtctttcag gttgtcgtag
    13981 ctgccgaagc cggcgccgcc gcccgagccg tagccgttgt tcagcgggtt gttgcggttc
    14041 caccagttct gcgggccgtt ctcctgcctc atccagcgca gcatgaagtc gacgttctct
    14101 tgtgtgagcg gccagccgcc gtccaccaag acgagacgtg cccagtcggc gttggtgctg
    14161 aaggttatga cgatgttgcc cgcctcggcg gtgagcccgt cgtggcccag ttgggcggaa
    14221 tgcacgtatc cggcgacgag aaagctctgt gccggcacgt gagccccgga agccgtgatc
    14281 tcctcggcgt gcgcggccgc cgtcgagggc agggagagcg ccgccgtcag cgccaacgac
    14341 agcgtcacga tgccccacgc cggcgtggag cgcgtcaaca cgtgccgatg agccccggcg
    14401 ggggaggcgc cctggcgggt cggcgcgagc ggaaccggcc gggcgcgtgc cgggcgggcc
    14461 ggcccggcac gatgcaggtg tggcgtgcga cggggagaga ccggcacggc cgcgtggcgg
    14521 ccgtgcgtcg ggtgcagcgg ttcgtgtggc ataccgggca ataggttagc aagtcgccgg
    14581 gccgggcttc ctgggtatac tcaaaaagtt ctgctcacgc ggcgcgctgg aggttatcgt
    14641 gatccgcctt ccggcccgcg cagcctcggc acgtgcccat gcgaaggcga tcctcatcgg
    14701 ggaacacgcg gtggtctacg gcgggcccgc gatcgcgctg ccggtgcccg gcctcacgct
    14761 caccgcagag gtcgtgcctg ccgacgggcc ggcgcgggtc gagtcggctc tctattccgg
    14821 gtcgttcgag aacatcccgc cgcggttcgc ggcacccgcc accgcggcag ccgccgcgct
    14881 ggagttggtg ggcgccggcc cgaccggctt tcgcctgcgg atctccgggc ggataccgcc
    14941 ggcccgcggt ctcggatcga gcgcggcggt cgccgcggcc atcagccagg cgattgccga
    15001 cgcctacgag gcggagctgg gcgaagagga gcactacgcg ctcgtgcagg ctgcggaacg
    15061 tgtcgcccac ggcacgccga gcggccttga cgcccgggcc gtgctcgcac ccggcgcgat
    15121 ctggtttcag gcaggcctct cgagcccgct tacggcaggg ccgggcgggg tgttcgtcgt
    15181 ggccgacacg ggcgaagcgg gtggcaccgc cggggcggtg gccgcggtgc gtcggctgaa
    15241 ggccgccgac gaggcccgcg tgcgcggcgt cttgggcgac ctcgacgccc tgaccgccga
    15301 ggcagcacag agcctcgtcg caggcgacac ggcggctctc ggggcggcta tgacggccgc
    15361 gcacacgctg ctcatccgcc tgacggtcag cagccccggc ctcgaccacc tcgtccgtgc
    15421 agcgcgggag ggcggcgccc tcggtgcgaa gctcacgggc ggcggccgcg gcggctgcat
    15481 ccttgccttg gccgaaggcc gcgcggctgc cgagcgcttg gccgaagctc ttcgcgcggc
    15541 cggcgccgcc gccacctgga ttcttggcgc cccgtgaccc cggctctcgc agaggcgacg
    15601 gcgctcgcgc acccgaacat cgcgctcatc aagtattggg gcaaagagga cgacgacctc
    15661 gtcctccccg cggccggcag cctctcgctc accctcgacg tattcccgac gacgacgacg
    15721 gtcacgccgg ataccggcct cacgcgcgac gtcgtcgagc tcgacgggcg cccggcgggc
    15781 cccacgacgg cgtaccgtgt cggcgccttc ctcgacctcg ttcgcgccct cgccggccgg
    15841 gccggcgcga agcctctgca cgcacgggtc cgcacccgca acgcggtgcc gaccggtgcc
    15901 ggcctcgcct cctccgcctc gggcttcgcc gcgctcgctc ttgctgcctc gtcggcctac
    15961 ggcctacggc tcgaccggcc gggccttggc aggctcgccc ggcgcggttc tggttcggcc
    16021 tgccgctcga tcgtgccggg cctcgccctc tggcgggccg gcgacgacgc ctcgtcttac
    16081 gcggtccccg tcgagggccc cccgctcggc atggtcatcg tcgtggccga cgtgggcggc
    16141 aagaaggtct ccagccgcga ggcgatgcgc cgcacccgcg acacgtcgcc gtactacgcc
    16201 gcctgggtgc gctctgccgc ggccgacctc gggccggcgc tcgaggcgct ccggcacgac
    16261 gacctggcgg cgctcggcga gctggtcgaa tcgaacgcgc tgcgcatgca cgcggccatc
    16321 gccgcctgcc ggccgcccat ccgctatctg cgcccggaga gcctcatggt cttcgaggcg
    16381 gcagaggaac tgcgtgtcag cggactgggt gtctacgcga cggcggatgc gggcccgaac
    16441 gtcgtgctcg tctgccgact tcgcgacctc gcccgggtgt cagaggcgct ggtcggccgg
    16501 ttggggcagg ccttcgcggg ccggatcgtg tcggccgggc ctgggccggg cgcgcgcctc
    16561 gtgcccgtcg gcacggcggc ggaggacccg gcccgcggtg cgccgcctgc ggtccggggg
    16621 cgcccgtgat ccgcgccacg gcccccggca agctcttcgt cgccggcgag tatgccgtcg
    16681 tcgagccggc gcagccggcc gtgctggtcg cggtcgaccg ctatatcacc gtcgcggtcg
    16741 acccgtcgcc gaccaccggc gccggccggc tcgtcagcac cgtcacctcc cccggcaggg
    16801 ccgcggcgac gtggcatcgc gccgggcccg gtatcgagac ggcgggcggg accgcggcgt
    16861 ttcgctacct gcttgccgcg gtcgcgatcg tcgaggagct ggccgccgag cggggcgtgc
    16921 cgttcagtgg ctgccggatg cgcgtcacga gcacgctgga gggcgcgggc cgcaagctcg
    16981 ggctgggctc ctccgccgcc gtcaccgtcg cggccgtgcg cgcgctgggc gagttctacg
    17041 gcctgggtct cgcgcggatg gccgttctca aggccgcgtt ggccgccacc ttccgcgtgg
    17101 cgcctgaggc ctctggaggg gatgtcgcct cgagcgtctt cggcggctgg atcgtctacg
    17161 cctccccgcg gcgtgaggcg ctgggcggta tgggcgtggc agcggcagaa ctcgtcaccc
    17221 gcgaatggcc gggcctgtgc gtgcggcacc tgccggcgcc ggcgggcctc cgccttctcg
    17281 tcggatggac gggcaacccc gccgtcaccg gccggcttgt cgccgacgtg cgggggcgag
    17341 ccggcggcac gccgcgagac ttcgtcgccc agagccggcg cgccgtgctg gggctcgtcg
    17401 gcgccttgca agatggcgac gccgaccgcg tgctcctcga ggtgcggcgc tgccgcgcgc
    17461 ttctggccgg cctggccggc cagacagggg tcgcgatcga gacgccgctt ctcgccgccc
    17521 tctgcgacgc cgccgagggc gagggagccg ccgccaaacc ctccggggcg ggcggcggag
    17581 actgcggcat cgcgctggcg ccgccggggg ccgatgccga ggccatactg caagagtgga
    17641 tcagacgggg catccgcccg ctcgacctgc ggcccgcgcc gaacatcgac tgagggtcgg
    17701 accacgacgc tggcggcggg ccgcgtcggc aaagaagggg gaggggcgat gagcgccgaa
    17761 cgcaaagacc agcacctcgc gctggccgac gagcagcagc tcggcgggcc ggcgcgcaac
    17821 gatttcgacg acgtgcggtt cgtgcaccac gccctggccg gcatcgaccg tgacggcatc
    17881 cgctccgccg tgtcgttcgc gggcctccgg tgggcccggc cgctctacat caacggcatg
    17941 acgggcggca gcgcgcgcgc cggccgggtg aatcgcgacc tcgcagtcgc ggcccgtgag
    18001 acgggcgtgc cgatcgcttc cggctcgatg agcatctgct tcaagggcga ggccgatgag
    18061 gcgacactcg acacgttccg cgtcttgcgg cgggagaacc ccgacggttt cgtcatggcc
    18121 aacctgagcg ccgacgcgac ccccgacgag gcggcgcggg ccgtcgacct gctggcggct
    18181 gacgctctgc agatccacct caacagcgtg caagagaccg tcatgccgga gggcagccgg
    18241 ggtttctcgg cgtggcccga ccagatcgcc gcgatcgtcg aggccgtcga aactcccgtc
    18301 atcgtgaagg aggtcggttt cggcctcagc cgggcgagcc tcgaaaggct cgccttgttg
    18361 ggcgtcggca tcgccgacgt gagcggtcgg ggcggcaccg acttcgcgcg catcgagaac
    18421 gcccgccggt cgggtggggc cgcacctgcc ggcaccctcg gggcgggcga tgccggcttc
    18481 ggttacgggt tcatggccgg ctggggccag tcggcggtgt gcagcctgct cgacgcgaag
    18541 gccggcgacg agggcgtgcc cgcccttctc gcctccggcg gggtgcgcac ccccctcgac
    18601 gtcgtgcgtt cgctcgcgct cggcgcgcac gcggtcggcg tctccggcgt gttcctgcac
    18661 gcggtgctgg agggcggcgc ggcgggcctc gtcgcgatga tccgcgcctg gctggagcag
    18721 atcgcgaagc ttcaggcact cctcggcgcg gcgagccccg cggccctcac cggcaccgac
    18781 gtgctcctca ccggcgccgt gcgggagtat tgccaactac gcggcatcga cgccgcctcg
    18841 tacgcccgcc gcagcgaacg ccccggggcc ggccggtgag ccgcgcggga gcggccgccg
    18901 tgcagggcgg cggggcgcgg gaggagcggg aggccggccg gccggccgag acggcaccga
    18961 tcccgctctc gtgggtcggc ccgctgctgc tgcgcgggcc cgtcgcctcc ggagagcagt
    19021 gggtgccgct ggcaaccttc gagaccccgc tgtggccctc cgtgcgccgc ggcgccaaga
    19081 tcagcacgat gaccgggggc atcctcacga ccctcgtcga cgagcggatg acccgctccg
    19141 tgattctcga ggccggcgac gccggcgagg cgctggcggc ctggcaacgc atcgacgcgg
    19201 cgcgggaaga gctgcaggca ctcgtcgccc aggtgagcgg tcacgcccgg cttctgggcc
    19261 tcaaccgtca gatcgtcggc aacctgctct ttttgcgcct tgagctcgcc gtcggcgacg
    19321 cggccgggca caacatggtc acgctcgccg ccgaccgcat catcgaccac atcctgaccc
    19381 gcctccccgg cctccgctac ggctccgtct ccggcaacta ctgcaccgac aagaaggcga
    19441 gcgccgtcaa cgggatcctc ggccgcggca agaacgtcgt cgccgagctg aacgtgccgc
    19501 gccgcctcgt cgaacgcctc ttgcacacga cgcccgaggc gatctcgacc ttgaacgtgc
    19561 gcaagaacct catcggcagc acgctcgccg gcagcatccg caccgccaac gcccactatg
    19621 cgaacatgct gctcgccttc tttctcgcca cgggccaaga tgcggcgaac atcgtggaag
    19681 gctcgcaggg cataacccat gcggagagtc gggaaggcga cctgtacttc tcgtgcaccc
    19741 tgccgaacct tgtcgtcggc acggtcggca acggcaagga cctgccattc gtggcccgca
    19801 acctcgaacg cctcggctgc cgcaccggca gcacggacgg caccgacgca cgtaggctgg
    19861 cggtgctgtg cgccg
//
LOCUS       DASTNL010000124         5341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_460885, whole genome shotgun sequence.
ACCESSION   DASTNL010000124 DASTNL010000000
VERSION     DASTNL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460885"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFQ96_03975"
     CDS             <1..838
                     /locus_tag="VFQ96_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEU0256979.1"
                     /translation="LREFRVGETDVTEPYPATSTPPSGLGVVLVPWPNRVKDGRWVLD
                     GVEQQLALTEPDRQNAIHGLLRYSPYRLVSRDANRVTLGAEIFPQLGYPFHLQTEVTY
                     ALSDAGLTVEHLLHNAGDRRAPVAVGAHPYLKIGGVPTEDLTLTVRADRHFEVDERLN
                     VVGEHPVEGTAFDLRAGRRVGDLTLDDGWSDVQQDEAGYGRHTVAAPDGRSVTLWSDH
                     NFGYVQAYTSRSLATLPAGQVAVAVEPMSAPTNALNTGRSLTWLEPGDTWRPRWGIGF
                     SL"
     gene            complement(904..3399)
                     /locus_tag="VFQ96_03980"
     CDS             complement(904..3399)
                     /locus_tag="VFQ96_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0256980.1"
                     /translation="MASLLYRLGRFAARRRWSVLITWLVALALAGVGFSLFAGTVSSS
                     ISLPGTPTEKVTSQLTKALPDTGAGSGSLVFATTDGHAFTRAQKKAVGTLLHDVARFD
                     GVAGTTNPFTAQQKLTDQRATIAQSATKIADGTRQVADARAQLDQAQKQLDTQRRQAE
                     AAGIPGAAAQLDGAQKTIDANSAKLDAEAAKLAKAKATLDLGRRLLKLTGDAGVVSHD
                     GSAAIGTVSFDRAITAVPAGLKDSIVDAADHADIAGVTVSVSNEIAETIPSVIGIGEI
                     VGVLIAALVLVLTLSTVIGAAIPLVSALFGVGVAVLAAMSFSSIVEFVSVTPVLGLML
                     GLAVGIDYSLFILNRHRKQLKQGFDVHESIGLANGTSGNAVVFAGSTVIVALLALNVT
                     GISFLGLMGTVGAVAVAVAILIAVTFTPAVLSLVGKHILRKGERARIGDPAAVRIPDR
                     PMKTWRAIVTLVAGMAVLGVVALPATQMRLGLPDGASQPTNSSQYRAYQVVNEKFGAG
                     HNAPLVVLATLPEKATGDAMLRRQVQIGEKLAAQSDVDAVVPIGVGHDNVSAAFEVVP
                     GTGPASEKTEQLVHDLRGLSPLTTASGPVTIGVAGNASANIDISDKLAGVLPLYLALV
                     VGLSLIILIIVFRSFLVPLTATAGFVLSLLAAFGGLTAVYQFGWLGAVFGVHDPAPIL
                     SFLPIIEVGVLFGLAMDYELFLVSGMREAFAHGSPARLAVQQGFHAGRSVVTAAAIIM
                     ISVFAGFVFSDSTMIRPVGFGLAFGVLVDAFVVRMLLIPSAMHLLGKAAWWFPRWLDR
                     LLPDVDVEGAKLERRAPAGQPEAPTTTQPVPTP"
     gene            complement(3500..4135)
                     /locus_tag="VFQ96_03985"
     CDS             complement(3500..4135)
                     /locus_tag="VFQ96_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0256981.1"
                     /translation="MEANATDRRAALKARHREAILEAARALVVERGGPRFSVDELADR
                     ADVARRTVFNHFASLDDVLLTVCADELGVVADEFTASVSALPVGDGSRAAVFDELTVA
                     IHAADLPSAIRRITSLLDIRDANDRRAGWVADTAFSRVGELLRQEISRRYKKADPLDV
                     QLLVGSLMSGLAVIAAHWIADTGASLDDDSRTRWHTLLTRLIDSIRSGYMP"
     gene            4304..4873
                     /locus_tag="VFQ96_03990"
     CDS             4304..4873
                     /locus_tag="VFQ96_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0256982.1"
                     /translation="MNGSRMGQGFGGRGFGGGRAGAGPHMADAFDQLRALFEQRVRPR
                     MARGDVRAAILALLTEKPMHGYQMIAEIEERSGGAWKPSAGSVYPTLQLLAEEGLIDS
                     EVADGRKTYSLTEAGRAEAESAAQGAAPWESAGRREPFGAGGLPRAGVELAQAAAQVR
                     RTGTPEQVEAAVTVLDEARRKLYSILAQD"
     gene            4878..>5341
                     /locus_tag="VFQ96_03995"
     CDS             4878..>5341
                     /locus_tag="VFQ96_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU0256983.1"
                     /translation="MPSAHGHRGNPTNDAAVTRARYRRIVRFALGALAQTWWYEVFLP
                     RVGLSRVTERGRTRRLTRVARRFHALALELGGLMIKVGQFLSSRLDVLPPEITRELAG
                     LQDEVPPAPFPPIGQLAEAELGMPLEHAYASFGEQAVAAASLGQVHRARLGAR"
BASE COUNT          827 a         1776 c         1921 g          817 t
ORIGIN      
        1 gttgcgggag ttccgggtgg gcgagaccga cgtcaccgag ccgtatccgg ccacgagcac
       61 gccgccctcc gggctggggg tcgtcttggt cccgtggccg aaccgggtca aagacggccg
      121 ttgggttctc gacggcgtgg aacagcagct cgccttgacg gagcccgatc ggcagaacgc
      181 catccacggc ctcttgcggt acagccctta ccggctcgtc tctcgcgacg cgaaccgcgt
      241 gaccttgggt gccgagatct tcccgcagct gggttacccc tttcatctgc agaccgaggt
      301 gacgtacgcg ttgtccgacg cgggcctcac cgtcgagcat cttctgcaca acgcgggcga
      361 ccggcgggcg ccggtcgcgg tcggcgcgca cccctatctg aagatcggcg gcgtgccgac
      421 cgaagacctg accctgacgg tgcgcgccga ccggcacttc gaggtggatg agcgcttgaa
      481 cgtggtcggc gagcacccgg tggagggcac cgccttcgat ctgcgggccg gtcgccgggt
      541 cggcgatctg accctcgacg acggctggtc cgatgtacag caagacgagg cgggatacgg
      601 ccgtcacacg gtggccgcgc ccgacgggcg cagcgtgacg ttatggtctg accacaactt
      661 cggctatgtg caggcgtaca cgtcacgcag cctcgcgaca ctgcccgccg ggcaggtggc
      721 ggtcgcggtg gagccgatga gcgcgccgac caacgccctg aacacgggcc ggtcgctcac
      781 ctggctggaa ccgggagaca cgtggcgacc ccggtggggc atcggcttct cactctgaag
      841 cctcccgggc caggctcgcg cgtcgcccgc gcctgagcgc agccggccgt acacctctcg
      901 cggctatggc gtcggcaccg gctgggtcgt cgtcggtgcc tccggctggc ccgcaggagc
      961 acgcctttcg agcttcgcgc cctccacgtc cacgtcggga agaagccggt cgagccacct
     1021 cgggaaccac cacgccgcct tgccgagcag gtgcatggcg gaggggatga gcagcatccg
     1081 cacgacgaag gcgtcgacga gcacgccgaa cgcgagcccg aagccgaccg gccggatcat
     1141 ggtcgagtcg gagaagacga atccggcgaa gacggagatc atgatgatcg ccgcggcggt
     1201 gacgacggaa cggcccgcgt ggaacccctg ctgcacggcc agccgagcag gcgagccgtg
     1261 cgcgaacgcc tcacgcatgc ccgaaacgag gaacagctcg tagtccatcg cgagcccgaa
     1321 cagcacaccg acctcgatga tcggcaagaa gctcaggatg ggcgccgggt cgtgcacgcc
     1381 gaagaccgcg cccaaccagc cgaattggta gaccgccgtc aggccgccga aggctgcgag
     1441 caatgacagg acgaaccccg ccgtggccgt cagcggcacg aggaacgagc ggaacacgat
     1501 gatcaggatg atgagcgaaa gaccgaccac aagcgccagg tagagcggca gcacgccggc
     1561 gagcttgtcg gagatgtcga tattggcgct ggcgttgccg gccaccccga tcgtcaccgg
     1621 cccggaggcg gtcgtcaagg gggagagacc gcgcaggtcg tgcacgagct gttccgtctt
     1681 ttcgcttgcg ggcccggttc ccggcaccac ctcgaaggcc gccgagacgt tgtcgtgccc
     1741 gacgccgatg ggcacgaccg cgtcgacatc cgactgtgcg gcgagcttct cgccgatctg
     1801 tacctggcgg cgaagcatcg cgtcgccggt ggccttctct ggcaacgtgg cgagcaccac
     1861 caagggcgcg ttgtgccccg caccgaactt ctcgttgacc acctggtagg cgcggtactg
     1921 gctgctgttc gtcggctggg aggcgccgtc gggcaggccc aaccgcatct gtgtggccgg
     1981 cagcgcgacg acgcccagca ccgccatgcc ggcgaccaac gtgacgatcg cccgccaggt
     2041 cttcatcggc cggtcgggga tgcgcaccgc ggccgggtcg ccgattctcg cgcgttcgcc
     2101 cttgcgcagg atgtgcttgc ccacgaggga gagcacggcc ggcgtgaagg tcacggcgat
     2161 caagatggcg acggcgaccg ccacggcccc gaccgtgccc atcagcccga ggaacgagat
     2221 tccggtgacg ttcagcgcca agagcgccac gatcacggtg ctgccggcga agacgacggc
     2281 gttgccggag gtgccgttgg cgagcccgat cgattcgtgc acgtcgaagc cttgcttcag
     2341 ctgcttgcgg tgccggttca agatgaacag cgagtagtcg atgccgaccg cgagccccag
     2401 catgaggccg agcaccgggg tcacggagac aaactcgacg atgctcgaga acgacatggc
     2461 cgccagcacc gcgacgccga cgccgaagag cgcgctcacg agcgggatgg cggcgccgat
     2521 cacggtgctg agggtgagga ccaagacgag tgccgcgatc aacacgccga cgatctcgcc
     2581 gatgccgatg acggagggga ttgtctcggc gatctcgttc gagaccgaga ccgtcacgcc
     2641 cgcgatgtcg gcgtgatcgg ccgcgtcaac gatcgaatcc ttgagacctg cgggcaccgc
     2701 ggtgatcgcc cggtcgaacg agacggttcc gatggccgcg ctgccgtcgt gggagacgac
     2761 tcccgcgtcg ccggtgagct tcaggaggcg tcggccaagg tcgagcgtgg ccttggcctt
     2821 cgccagcttg gcagcctccg cgtcgagctt cgccgagttc gcgtcgatcg tcttctgcgc
     2881 gccgtccaac tgtgcggccg cgcctgggat gccggcggcc tcggcctggc ggcgctgtgt
     2941 gtcgagttgt ttctgcgcct ggtcgagctg ggcgcgggca tccgccacct gccgcgtgcc
     3001 gtcggcgatc ttcgtggcgc tctgggcgat cgtcgcgcgc tggtcggtga gtttctgctg
     3061 cgcggtgaag ggattcgtgg tgcctgccac gccgtcgaag cgggcgacgt cgtgcagcaa
     3121 ggtgccgacc gccttcttct gggccctggt gaaggcgtgc ccgtcggtcg tcgcgaagac
     3181 gaggctgccg ctgcctgccc ccgtgtcggg cagggccttc gtcaactggc tcgtcacctt
     3241 ctcggtgggt gtgcccggca ggctgatcga gctggagacc gtgccggcga acaggctgaa
     3301 tcccacgccg gccagcgcga gcgcgacgag ccaggtgatg aggacgctcc agcgtcggcg
     3361 ggccgcgaac cggcccaggc ggtacagcag ggaggccatg tgtgccccgt ttctttcgtt
     3421 gcgacggccg tggatgggcc ggttgtggca ggacccgacg tcggatcctc aggtctgggt
     3481 gtgagtgtgc gggtcggcgt cagggcatgt atcccgaccg gatgctgtcg atcaggcggg
     3541 tgagcagggt gtgccatcgg gtgcgggagt cgtcgtcgag gctggcaccg gtgtccgcga
     3601 tccagtgtgc ggcgatcacg gcgaggccgc tcatcagcga gccgacgagc agctgcacgt
     3661 cgagcgggtc ggccttcttg tatcggcggg agatctcttg ccgcagcagt tcgccaaccc
     3721 tgctgaaggc ggtgtcggcc acccaaccgg cgcgtcggtc gttggcgtcg cggatgtcga
     3781 ggaggcttgt gatccgtcgg atcgcactcg gcagatcggc cgcgtggatg gccaccgtga
     3841 gctcgtcgaa cacggctgcc cggctgccgt cgccgacggg cagggccgag accgaggccg
     3901 tgaactcgtc ggccacgaca cccagctcgt cggcgcagac cgtgagaagc acgtcgtcga
     3961 gagaggcgaa atgattgaac accgtgcggc gcgcgacgtc tgcgcgatcg gcgagctcgt
     4021 cgacggagaa ccgcggcccg ccccgttcta cgacgagcgc ccgtgccgcc tcgaggattg
     4081 cctcgcggtg tcgggccttc agggccgcgc ggcggtcggt ggcgtttgcc tccatcacat
     4141 gattacacta agtgcatcga cgcacgttat gcaatttatc tcggcgttcg cacagcaaag
     4201 atgcgtcgat ctctgagcat gccgtgacgg gtattcgccg gccgaacgat atatcgtagt
     4261 gccacacgag atgggtaatg cctctctgaa cgaggaggtc gtcatgaacg gttcacgcat
     4321 gggccagggc ttcgggggcc gcggtttcgg cggcgggcgc gccggcgccg gcccgcatat
     4381 ggcagacgca ttcgaccagt tgcgcgcctt gttcgagcag cgcgtcaggc cgcggatggc
     4441 ccgcggcgac gtgcgtgccg cgattctcgc cctcttgacg gagaagccga tgcacggcta
     4501 ccagatgatc gcagagatcg aagagcgcag cggcggcgcc tggaagccga gcgccggctc
     4561 ggtgtatccg acgttgcagc tgctggccga ggagggcctc atcgactctg aggtggcgga
     4621 cggccgcaag acctattcgc tgaccgaggc gggccgggcc gaggcggagt ctgcggcgca
     4681 aggagccgcg ccgtgggagt cggccggccg cagggagccc ttcggcgccg gcggtcttcc
     4741 gcgggcgggc gtcgaactgg cccaggcggc ggcgcaggtc aggcgcaccg gcacccccga
     4801 gcaggtggag gcggccgtga cggtgctgga cgaggcccgc cgcaagctct actcgatcct
     4861 cgcccaggac tgaccggatg ccctcggccc acggtcatcg agggaacccg actaacgatg
     4921 cggcggtgac gcgtgcgcgc taccgccgca tcgtccggtt tgcgctcggc gccctcgctc
     4981 agacctggtg gtacgaggtg ttcctgccac gcgtcgggct gtctcgcgtc acggaacggg
     5041 gacgcacccg gcggctgaca cgtgtcgcga ggcgcttcca tgccctcgcc ctcgagctcg
     5101 gcggcctgat gatcaaggtc gggcagttct tgtcgtcccg gctcgacgtg ctgccgcccg
     5161 agatcacgcg ggagctcgcc ggcctccagg acgaggtgcc gcccgccccg ttcccgccga
     5221 tcgggcagct cgccgaggcg gagctcggga tgccgctcga gcacgcgtac gcctcgttcg
     5281 gcgagcaggc ggtggccgcg gcgtctctcg gtcaggtaca ccgcgcccgc ctgggcgcgc
     5341 g
//
LOCUS       DASTNL010000125         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_461140, whole genome shotgun sequence.
ACCESSION   DASTNL010000125 DASTNL010000000
VERSION     DASTNL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461140"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VFQ96_04000"
     CDS             complement(<1..1263)
                     /locus_tag="VFQ96_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU0256984.1"
                     /translation="MTNTAAARRPASRGRAAVWITIGILVALVIAFFIFAGLYANWLW
                     FQQIGFLSVLTTQWLAGSVLFVIGFFGMGLPLWVTVMVAYHARPLYAKLDAQVDRYRQ
                     VVEPLRRLAMYGIPVIFGLFGGVSAASHWQQTLLWLNATPSPVKDPQFHLSVSFYMFD
                     LPFLQGLVGFASAAVIVSIIAVLATSYLYGGIRIQGREVHVSRAARVQISVTAAVYLL
                     LQALSVWLDRYVTLTDSNVNGMINGAAYTDVHAIIPGRTILTGIAILVALLFVFNVFN
                     GRWRFPLVGTALLLVSALIIGGIYPWVVQKFQVVPSEKSLEVPYVKRAIEFTRAAYGV
                     SDVKEQDYNAKTDAKPGALRKDAETTAEIRIIDPNEVSESFAQLQQFKQYYGFPKTLD
                     VDRYKIDGKTQDTVIAVRQMEQSGLSSRN"
     gene            complement(1403..2608)
                     /locus_tag="VFQ96_04005"
     CDS             complement(1403..2608)
                     /locus_tag="VFQ96_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0256985.1"
                     /translation="MPGAEPPVPVGPVPGLPGAASPGARQVGGPGPAPVRRGTPRRVI
                     VGWVSLALGVVLLIALGLTPSPYVIEEPGPVFNTLGVDAPVHGSSAAGVDVARNPRPV
                     ITIDGHPTYPTSGGLYMLTVSSYGQPGRLPNWLNVVSAWFAPTDAVIPVDVAYPPATT
                     VTEQNRANEALMRSSQEDATAAALHHLGIPVGRRVVVETVTPGMPAAGVLKPADRITA
                     VDGRPVGTVSALKKALAAGGPGVARLGIVRAGVHREVTLRPRLQHGSRVIGVGVTTAY
                     SFPFTVKIRLNEVGGPSAGMMFTLGILDKMTPGKLNGGHRVAGTGTIDEEGTVGAIGG
                     IEQKMAAARAAGARYFLAPRGNCPQVVGHVPKGLQVFAVSNLDGALAVLHAVRTGSST
                     SALPRCPAA"
     gene            2849..4303
                     /locus_tag="VFQ96_04010"
     CDS             2849..4303
                     /locus_tag="VFQ96_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU0256986.1"
                     /translation="MADHSDEDDDFESILRSFLSGSGPIDASRLAGAAGLPADPTGVA
                     WLFASLQDAVRQGDDSIHWEPATQQAERLAADGARPVDEKTSAGVDAAFHVAGLWLSE
                     TVQIAELTAPPASMTRTEWVRQTMQLWVQMAEPVALSISDAITRVFREQAPEQLRGMA
                     GGAEQAIRAVGGALFALQLGQVVGQLSREVVSGGDIGIPLLEAGRAGVVPQNIEGFGE
                     GLDIPADQVQLYLAVRELAHARLFRHARWLRLQMISAITAYAKGITIDTGQLQQLAEG
                     FNPSNPDELRQILADGTLIPPKSAAQEAALTRLETTLALIEGWVDVVTRRATTRLPKS
                     DAVAETVRRRRAIGGPAERAFATLVGLELRPRRLREAAALWEAVTEAVGPEGRDALWS
                     HPDLLPTAADLDDPTRLVDQLRERQAGHVPEPDDLDKALADILAADGDGGDPSASGPG
                     PAAGTGPQGPGPAQGPESSDDNDGPHGTGEPDRG"
     gene            4372..>4711
                     /locus_tag="VFQ96_04015"
     CDS             4372..>4711
                     /locus_tag="VFQ96_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256987.1"
                     /translation="MNATPAILQLTDSLPRVWRSPDELQFGLDRPALVLRHVTNLEER
                     LIAALDAGVTRAGLTMLARRAGGKDAQVTELLERLRPVLGRPPRPSGPPRPGNVAVAG
                     GGPVGDLVAHL"
BASE COUNT          771 a         1628 c         1665 g          647 t
ORIGIN      
        1 gttgcgggac gagagccccg actgctccat ctggcgcacg gcgatgacgg tgtcttgcgt
       61 cttgccgtcg atcttgtacc ggtcgacgtc gagggtcttg gggaacccgt aatactgttt
      121 gaactgctgc agctgcgcga acgactcgct gacctcgttc gggtcgatga tgcggatctc
      181 cgccgtcgtc tcggcgtctt tgcgcaacgc cccgggcttc gcatcggtct tcgcgttgta
      241 gtcctgctcc ttcacgtcgg agacgccgta tgccgcccgg gtgaactcga tcgctcgctt
      301 gacgtaaggc acctccagcg acttctcgct cgggacgact tggaacttct gcaccaccca
      361 cggatagatg ccgccgatga tgagggccga gacgagcaag agggcggtgc cgacgagggg
      421 gaagcgccac cgaccgttga agacgttgaa cacgaagagc aaggcgacca aaatcgcgat
      481 gccggtcaga atcgtgcggc ccgggatgat cgcatgcacg tcggtgtatg cggcgccgtt
      541 gatcatgccg ttgacgttcg agtcggtgag cgtgacgtac cggtcaagcc acacgctcag
      601 agcctggagc aacaggtaga cagccgccgt caccgagatc tgcacccgcg ccgcgcgcga
      661 gacatgcacc tcccgcccct ggatgcggat gccgccgtag aggtagctcg tggcgaggac
      721 ggcgatgatg gagacgatga cggcggcgga ggcgaaaccg acgagaccct gcaggaacgg
      781 caagtcgaac atgtagaacg acacgctcag gtggaactga gggtccttga ccggactcgg
      841 cgtcgcgttc agccagagca gcgtctgttg ccaatggctc gccgcagaaa cgccgccgaa
      901 caagccgaag atgacgggaa tgccgtacat ggccaggcgc cgcagcggct cgacgacctg
      961 acgatagcgg tcgacctgtg catccagctt ggcgtagagc ggcctcgcat ggtaggcgac
     1021 catgaccgtc acccacaacg gcaggcccat gccgaagaat ccgatgacga acaagaccga
     1081 gccggccagc cactgcgtcg tcaagacgct caagaagccg atctgctgaa accacagcca
     1141 gttcgcgtac agccccgcga agatgaagaa cgcgatgacc agggcgacga ggatgccgat
     1201 ggtgatccag accgcggcac gcccccgcga agccggacgc ctggctgctg ctgtatttgt
     1261 cacgctgcga ttccctgtgg tcgaggcttg gggcggtcgc ctcccatcct agggtgcggc
     1321 cccaagaggg cgctgaacac gggacgagtg caggcgcgcc ccgccggcgg ccgccgtcac
     1381 gccgacgggc ggcgcggaat cgtcacgctg cggggcaccg cggcaaggcc gacgtcgacg
     1441 acccggtgcg caccgcgtgc agcacggcaa gcgccccgtc gaggttggag accgcgaaga
     1501 cctgcagacc cttcggcacg tgcccgacta cctgcgggca gttcccgcgc ggagccaaga
     1561 agtaccgggc gccggcggcg cgtgcggcgg ccatcttctg ctcgatgcca ccgatcgccc
     1621 cgaccgtgcc ctcctcgtcg atcgtgcccg tgcccgcgac acggtgaccg ccgttcagct
     1681 tgccgggcgt catcttgtcg aggatgccca gggtgaacat catgccggcg ctggggccac
     1741 cgacctcgtt cagtcggatc ttcacggtga acggaaacga ataggccgtc gtcaccccga
     1801 cgccgatgac ccggctgccg tgctggaggc gggggcgcag ggtcacctcg cggtgcacgc
     1861 cggcccgcac gatgccgagt ctcgccaccc cggggccgcc cgccgcgagc gccttcttca
     1921 aggccgacac cgtgccgacc gggcggccgt cgacggcggt gatgcggtcg gcgggcttta
     1981 agacgcctgc ggcgggcatg cccggcgtca cggtctcgac cacgacacgc cggccgacgg
     2041 ggatcccgag atgatgcaag gccgccgccg tcgcatcctc ctgggaggag cgcatcaggg
     2101 cttcgttggc gcggttctgc tctgtgaccg tcgtcgcagg cgggtaagcc acgtccaccg
     2161 ggatcaccgc gtccgtcggc gcgaaccatg ccgagacgac gttcaaccag ttcggcagcc
     2221 tccccggctg cccatacgac gagacggtga gcatatagag cccgcccgac gtcggatagg
     2281 tgggatgccc gtcgatcgtg atgaccgggc gggggtttcg ggcgacatcc acgcctgcgg
     2341 ccgacgagcc gtgcaccggc gcgtcgacgc cgagcgtgtt gaagaccggg cccggctcct
     2401 cgatgacgta gggagaaggg gtgaggccca acgcgatcag gaggacgacg ccgagggcga
     2461 gcgacaccca gcccacgatg acacggcgag gcgtgccgcg ccgcacgggg gccgggccgg
     2521 gccctccgac ctgccgggcg cccggcgagg cggcgccggg gagcccggga acgggcccta
     2581 cgggcacggg cggttctgcg ccaggcatgg aacgctcctt ccggctgtcg aggatatccg
     2641 gtgcaccccg agggccgccg gggccccgcc catggtaggc cgccggcatg ccgcccgagg
     2701 accacgggag gaaggccctg gcacggccag gcgcccgagg cacgcgaagg cttcggagca
     2761 acgacacagc cctcggcggg gcataggcgt tagcgtagtt gcggtgcgcg tattcctcgc
     2821 ccgggatgcg gaaggaaggc gaagtacgat ggccgatcac agcgacgagg acgacgactt
     2881 cgagagcatc ctgcgcagct tcctttccgg ctccgggccg atcgacgcca gccgtctggc
     2941 cggtgcggcc gggttgcccg ccgatccgac cggcgtcgcc tggttattcg ccagcctcca
     3001 ggacgccgtg cgtcaagggg acgacagcat ccattgggag cccgccaccc agcaggccga
     3061 acgcctggcg gccgacggcg cgcggccggt ggacgaaaag acctccgcag gcgtcgatgc
     3121 cgcgttccac gtcgcaggac tgtggctctc cgaaaccgtg cagatcgcgg aactcacggc
     3181 cccgcccgcg tcgatgactc gcaccgagtg ggtgcgccag acgatgcagc tctgggtaca
     3241 gatggccgag ccggtcgcgc tcagcatctc cgacgcgata accagggtgt tccgcgaaca
     3301 ggccccggag cagttgcgcg gcatggccgg cggcgcggag caggcgatcc gcgcggtggg
     3361 cggggcgctg ttcgccctgc agctcggcca ggtggtgggc caactctccc gcgaggtggt
     3421 ctcgggcggc gatatcggca tccccctgct cgaggccggc cgagccggtg tcgtcccgca
     3481 gaacatcgaa ggtttcggcg agggtctcga cattcccgcg gatcaggtgc agctctacct
     3541 cgccgtgcgg gagttggcac acgccaggct cttccggcac gcgcgttggc tgcgtctgca
     3601 aatgatctcc gcgatcaccg cttatgccaa gggcatcacg atcgataccg gccagctgca
     3661 gcagctggca gaggggttca atccctccaa ccccgacgaa ttgcgccaga tcctcgccga
     3721 cggaacactg atcccgccga agtctgcggc gcaggaggcc gccctgacaa ggctcgagac
     3781 gacgttggcg ctgatcgagg gctgggtcga cgtggtcacc cgtcgtgcga cgaccaggct
     3841 cccgaaatca gacgccgtcg cagagacagt gcgccgtcga cgcgcgatcg gcggcccggc
     3901 ggagcgggcg ttcgcgacgc tcgtggggct ggagctgcgg ccgcggcgtc tgcgcgaggc
     3961 ggccgccctg tgggaggcgg tgaccgaggc ggtcggcccc gagggccgtg acgccttgtg
     4021 gtcgcacccc gatctgctcc ccaccgccgc cgacctcgac gacccgaccc ggctggtcga
     4081 tcagctgcgg gagcgccagg ccggacacgt gccggaaccc gacgacctgg acaaggcgct
     4141 cgccgacatc ctcgccgccg acggcgacgg cggcgatccg tcggcttccg gcccgggccc
     4201 cgcggccggc acaggcccgc aaggccccgg cccggcgcag ggccctgaaa gctccgacga
     4261 caacgacggc ccacacggga cgggcgaacc cgaccgcggc tgagcgccag gcggccgtcg
     4321 cgggcctgtg gagaacgcct gccggcgccg gaggtcgggc acgatgggcg catgaacgcg
     4381 acgccggcca ttcttcagct gaccgattcc ctgccgcggg tgtggcgctc gcccgacgag
     4441 ctccagttcg gcctcgatcg gccggccctc gtcctgcggc acgtcaccaa ccttgaagaa
     4501 cgtctcatcg cggcgctcga cgccggggtg acccgggcgg gtctcaccat gctcgcccgg
     4561 cgggcgggcg gcaaggacgc ccaggtgacg gagctgttgg agaggctgcg gccggtgctc
     4621 ggccgcccgc cgcgcccgtc ggggccgccc cggccgggca acgtcgccgt cgcaggcggc
     4681 ggccccgtgg gcgacctcgt ggcacatctc c
//
LOCUS       DASTNL010000126         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_832209, whole genome shotgun sequence.
ACCESSION   DASTNL010000126 DASTNL010000000
VERSION     DASTNL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832209"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFQ96_04020"
     CDS             complement(<1..341)
                     /locus_tag="VFQ96_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256988.1"
                     /translation="MAPRTKPFVDGEPRGIGGWLRETRFGMLTVALTVGIVSGFGAVG
                     FRYLIIVFTWLSTGQIHPLPPGQVSSHLPFLGHWFLWVIPVVGGAVYGPLIHWLAREA
                     RGYGVPQVMMAV"
     gene            659..1192
                     /locus_tag="VFQ96_04025"
     CDS             659..1192
                     /locus_tag="VFQ96_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256989.1"
                     /translation="MGAEDDERAVARLRELLLWAVRELRAGQAPTEALARFESARRRM
                     LVTVKSKMVPLGRVWRLGVVLLDEQGGLYATGSLTRAVDPKWRQYVAQSMEVRRDRRG
                     AAFRGPFAAGESVDFDARPIEVDAAVLRESDGPVLLQGGRVVVLWAPGTKPRLFEDYL
                     RERVDLAVHPPEGDGRS"
     gene            1349..2239
                     /locus_tag="VFQ96_04030"
     CDS             1349..2239
                     /locus_tag="VFQ96_04030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0256990.1"
                     /translation="MSSTKRTPMTDVAASSPLSASGEFTTRRERRDFERRATRAASPL
                     RARKPPLLSVSRPAQRKPGHVRSGAFFLSMGFAAAMAVATSVPAVSLPAAAAARALPP
                     TPVAHASAGQSVTTGDTIVSQVATRDDFSVTTREQLAELSHIQMAGTFTNDPNGTVQW
                     PFPVGVPISSYFGPRIAPMPGASTFHEGIDFDPGPGAGVDAIADGVVTTAHASAVGGL
                     GVYVVIDHVIDGQKIASMYAHMRPGSLTVHVGQHVSVAQMIGRVGDTGISTGPHMHFE
                     ILENDVTPVDPYAWLKAHAN"
     gene            2284..2355
                     /locus_tag="VFQ96_04035"
     tRNA            2284..2355
                     /locus_tag="VFQ96_04035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Thr,seq:ggt)
     gene            2385..2461
                     /locus_tag="VFQ96_04040"
     tRNA            2385..2461
                     /locus_tag="VFQ96_04040"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2419..2421,aa:Met,seq:cat)
     gene            complement(2591..2935)
                     /locus_tag="VFQ96_04045"
     CDS             complement(2591..2935)
                     /locus_tag="VFQ96_04045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEU0256991.1"
                     /translation="MTDRELAPGVVAWASLEPVRGREQGGHRPVLVVASAGYLDAVTT
                     LVIALPITTVDRGWPNHIRVDGPSGLGRPSWIMTEQPRTLSRDRLTGIAGQVSPGCLE
                     QVHTWLGDFLDL"
BASE COUNT          475 a         1053 c         1080 g          459 t
ORIGIN      
        1 acggccatca tcacctgggg cacgccataa ccccgcgcct ccctggccag ccagtggatg
       61 agcggcccgt acaccgcgcc gccgaccacc ggaatgaccc acaggaacca gtgtccgagg
      121 aacggcaggt gggagctcac ctgccccggt ggcagcggat gaatctggcc ggtcgaaagc
      181 caggtgaaca cgatgatgag gtagcgaaag cccacggcgc cgaagccgga gacgatgccg
      241 acggtgagcg ccacggtcag catgccgaag cgcgtctcgc gcagccagcc cccgatgccc
      301 cggggctcgc cgtcaacgaa aggcttcgtg cggggcgcca cgctctcgcc tccgcacaac
      361 ctctcgtgcg ccaccctcgc gacgccttgg atcgcggccc tcgaagccgc ctgccacccg
      421 cccccgacgc ttctccgctg tcggcggccg ggcaggcgac gtgtggccct cttcctctca
      481 ctatggcatg cctgcgctat ggacttgctg aacagtggcg ggtcgaggcg cccaacccgg
      541 cgggcgacgc actgccatcg gggttcggcc ccacccctgc gggcgggcat gtgcggggca
      601 gcccgaagca ggtgacggtt cggccgcgcc ccggcgggcc gcgccgtaaa ctgcggctgt
      661 gggcgcggaa gacgacgaac gggccgtggc ccggctgcgc gaacttctct tgtgggcggt
      721 gcgagaactg cgggcgggcc aggcgccgac ggaggcgctc gcccggttcg aatcggccag
      781 gcgccgcatg ctcgtcaccg tcaaatcgaa gatggtgccg ctcggcaggg tgtggcggct
      841 cggtgtcgtg ctcctcgacg agcagggcgg gttgtacgcg acgggctccc tcacgagggc
      901 cgtcgacccg aaatggcggc agtacgtcgc gcagtcgatg gaggtgcgcc gcgaccggcg
      961 cggcgccgcc ttccgaggcc cgttcgccgc gggggagagc gtggatttcg acgcccggcc
     1021 gatcgaggtg gatgccgccg tgctacggga atccgacggc ccggtgctcc ttcagggcgg
     1081 ccgcgtcgtc gtgttgtggg ccccgggcac gaagccgcgc ctcttcgagg actatctgcg
     1141 ggagcgggtg gatctggcgg ttcatccgcc cgaaggcgac ggccgttcct gaacggcctt
     1201 cccgcaggcc ggcgccgagg cgttctcagc cgcccgttgg aattgtctgc ggtcagccgc
     1261 taacctggtg aggttcgcct ggccgcggta tcggcggcgg gcagcgacgg aacacccgaa
     1321 accgagagaa cggcagaaga acaacgctgt gtcgagcacg aagcgcacgc cgatgacaga
     1381 cgtcgccgct tcgtcccctc tttcggcatc cggagagttc acgacgcgac gggagcgtcg
     1441 tgatttcgag cgccgggcga cgagggccgc ctcccccctc cgcgcccgca aacccccgct
     1501 cctctcggtg tcccgcccgg cacagcgcaa gccgggtcac gtaagaagcg gagcgttctt
     1561 cctctcgatg gggtttgccg cagccatggc ggtcgcgacc tccgtgccgg ccgtgtcgct
     1621 gccggccgcg gccgccgccc gggctcttcc tccgacaccc gtggcccacg cctccgccgg
     1681 gcagagcgtg acgacgggcg acaccatcgt cagccaggtg gccacccgcg acgacttctc
     1741 cgtgacgacg agggagcagc tggcggagct cagtcacatc cagatggccg gcaccttcac
     1801 gaacgacccc aacggcacgg tgcaatggcc gttcccggtc ggggtgccga tcagcagcta
     1861 cttcggcccg cggatcgccc ccatgccggg cgcctcgacc ttccacgagg ggatcgactt
     1921 cgacccgggc cccggcgcgg gcgtcgacgc catcgcggac ggcgtcgtca cgacggccca
     1981 tgcctcggcg gtcggcggcc tcggcgtgta tgtcgtcatc gaccatgtca tcgacggcca
     2041 gaagatcgcc agcatgtacg cgcatatgcg ccccgggtcg ctgaccgtcc acgtcggcca
     2101 gcacgtcagc gtcgcccaga tgatcgggcg ggtcggcgac accggcatct ccaccgggcc
     2161 gcatatgcat ttcgagatcc tcgagaatga cgtgacgccg gtcgaccctt acgcctggct
     2221 gaaggcccac gccaactgag ccggatggta ttctcgtgag gttgccgcgc gacgcgcggc
     2281 ctcgccccct tagctcatcg gtagagcact tccttggtaa ggaagaggta gtgggttcga
     2341 ttcccacagg gggctccggg atgcgccagc cgcaccccga atgcggcggg atagctcagg
     2401 tggttagagc acacggctca taatcgtggt gtcgcgggtt cgagtcccgc tcccgctacc
     2461 aagcagaaca caatgggaac ggcggccgcg ggatggcctc gcctcggtgc ctctggtcac
     2521 ggtggcgaga gccattctgc tgtcggcgtg agagggctgg cggtgctcac ggcctatggt
     2581 gtcggggccc tcaaaggtcg agaaaatcgc caagccaggt atgcacctgt tcaagacagc
     2641 cgggggagac ctggccggcg attcctgtga ggcggtcgcg ggagagcgtg cgaggctgct
     2701 cggtcatgat ccatgagggg cggccgaggc cgctgggccc gtcgacgcgg atgtggttcg
     2761 gccatccgcg atcgaccgtg gtgatcggca gtgcgatcac gagggtcgtc accgcgtcga
     2821 ggtagccggc ggaggcgacg acgagcaccg ggcgatggcc gccctgctca cggccgcgca
     2881 cgggctcgag ggaggcccag gcgacaacgc ccggagcgag ttctcgatcc gtcacgaggc
     2941 ggagtcggca agttcggcac gttcccactc ggcggattcc accgcatgtg atgcggcatc
     3001 tgcggtggtg gtggcggcga tcgcggtctt caaagattgc agacgttcgc ggcgatcctc
     3061 ggcatcc
//
LOCUS       DASTNL010000127         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1571709, whole genome shotgun sequence.
ACCESSION   DASTNL010000127 DASTNL010000000
VERSION     DASTNL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571709"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="cysS"
                     /locus_tag="VFQ96_04050"
     CDS             complement(<1..1027)
                     /gene="cysS"
                     /locus_tag="VFQ96_04050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0256992.1"
                     /translation="MTLRLFDTRAGGMREFRSVHPHRVGIYVCGPTVQSSPHIGHLRS
                     VLVYDQLRRWLTYRGYDVTLVRNVTDIDDKILVNSAAERAEGSTEEWWALAYRYELEF
                     TASYVALGIQPPSYEPRATGNIPQMLELITRLIERGHAYPARDGSGDVYFDVASWPSY
                     GELTRQSIDDMVADDEAELRGKRDPRDFALWKGHKDDEPETASWPSPWGDGRPGWHIE
                     CSAMSSRYLGSHFDIHGGGLDLRFPHHENELAQSRAAGEKFAEYWVHNGLVTVDGQKM
                     SKSLGNSVYASELLAQAPAVVVRYYLGAAHYRSTIDFHDGSLTEARAALERIGGFLER
                     AGRVLTGE"
     gene            complement(1098..1580)
                     /locus_tag="VFQ96_04055"
     CDS             complement(1098..1580)
                     /locus_tag="VFQ96_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU0256993.1"
                     /translation="MLFEVGETVVYPHHGAATITELKKRTIKGEEKLYLKLKVTQGDL
                     VIEVPADNVDLVGVRDVIDEKGLKKVFEVLQAPFTEEPTNWSRRYKANLEKLASGDVI
                     KVSEVVRDLWRRDQDRGLSAGEKRMLAKARQILVSELALAEKTDEEHASSSLDEVLAS
                     "
     gene            1737..2249
                     /locus_tag="VFQ96_04060"
     CDS             1737..2249
                     /locus_tag="VFQ96_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0256994.1"
                     /translation="MTEDLVRGRVAASVILAACIAIGTAGCTFATNQDTKNIKETAFG
                     VSGATGDVSVHNAALITGEGSSANLVVTLVDSGTKSQVVTIQTPTKNGKSTQTVTVTP
                     ANPLQIGGQDGSQRIQFDSFGAAAGTLHDVYFQSGDGNGVALKVPVLTNQLPTYATLT
                     PTPQPAGTLQ"
     gene            complement(2551..3303)
                     /locus_tag="VFQ96_04065"
     CDS             complement(2551..3303)
                     /locus_tag="VFQ96_04065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HEU0256995.1"
                     /translation="MESSSHTLILLRHGESAWNKSNQFTGWVDVRLTEKGAAEAARAG
                     ELLRGAGLRPDVLHTSVLSRAIQTANIALEAADRLWIPVRRSWRLNERHYGALQGKDK
                     AQTLAQYGQEQFMAWRRSYDVRPPALGDDAEYSQAGDERYRELGDALPRTECLKDVVA
                     RLLPYWESAVTADLAAGKTVLVTAHGNSLRALVKHLDGIGDDDIAGLNIPTGIPLVYR
                     LDDDFHPLGAGEYLDPEAAAKGAAAVAAQGRA"
     gene            complement(3352..4416)
                     /locus_tag="VFQ96_04070"
     CDS             complement(3352..4416)
                     /locus_tag="VFQ96_04070"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEU0256996.1"
                     /translation="MTGAAGDDPFSRLPGAVLGAGWDEGVPAHYGNPLGEQRRLTERG
                     AIVDLSHRVVLRLAGAPGSAGAGRLLLERLGTADIVRASSAETLLLDPSGAVRHALHV
                     IDDGRAPWIIAEASQAGDLERRLGGEPGVALEDRSADFGVIGTFTAELPETASVDGVP
                     LVWHDPWPSAGAGGPRYAAAEPHPAQGWSWHESLVPRESLAALAGRVGAPGVAGALAF
                     DALRVEAWRPRQVTEVDGTLTPRELDWARTAVPRDKVGYPGARALRAAEARPAGRRLV
                     MLYLDGSESVLPRHGAPVWLEVGGERLVVGAVTTGAMHHELGPVALAVVEREVPVRAP
                     LNVDEAETVVPALQEQIVAA"
     gene            complement(4413..>4595)
                     /locus_tag="VFQ96_04075"
     CDS             complement(4413..>4595)
                     /locus_tag="VFQ96_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HEU0256997.1"
                     /translation="RIDLTTGGVLRGPGAALMSAGRRMYGLVDGHLLWAWDIAALGQD
                     LRTHASARLARVQAAA"
BASE COUNT          717 a         1619 c         1550 g          709 t
ORIGIN      
        1 gctcgccggt caagacccgc cccgcacgtt caaggaaccc gccgatcctc tcgagcgcgg
       61 cacgcgcctc ggtgagggag ccgtcgtgaa agtcgatcgt ggaacgataa tgtgcggcgc
      121 cgaggtagta ccgcacgacg accgcggggg cttgggcgag cagctcggag gcgtagacgg
      181 aattgccgag cgatttcgac atcttctggc cgtcgacggt cacgagcccg ttgtgcaccc
      241 agtactcggc aaacttctcg cccgccgccc tcgactgcgc caactcgttc tcgtggtgcg
      301 ggaagcgcag gtcgaggccg ccgccgtgga tgtcgaagtg gctgccgagg tagcgcgagg
      361 acatcgccga gcattcgatg tgccagccgg gcctgccgtc gccccacgga ctcggccaac
      421 tcgccgtctc cggctcgtcg tccttgtggc ctttccagag cgcaaagtcg cgggggtcac
      481 gtttgccccg cagctcggcc tcgtcgtcgg ccaccatgtc gtcgatgctc tgacgcgtca
      541 gttcgccgta ggagggccag ctggcgacgt cgaagtagac gtcgccagag ccgtctcggg
      601 cggggtaggc gtgcccgcgt tcgatgagtc gggtgatgag ctccagcatc tgcggtatgt
      661 tgcccgtcgc ccggggctcg tagctgggcg gctggatacc gagcgccacg tagctcgcgg
      721 tgaattccag ctcgtaccgg taggccagcg cccaccactc ctcagtgctg ccttcggccc
      781 gttccgccgc ggaattgacg aggatcttgt cgtcgatatc ggtgacgttg cgcacgaggg
      841 tcacgtcgta gccacggtag gtcaaccagc ggcgcagctg gtcgtagacc aacaccgaac
      901 gcaggtgccc gatgtgcggc gaggactgca cggtcggccc acacacgtag atcccgaccc
      961 gatgcggatg caccgatcgg aattcacgca tcccccccgc gcgggtgtcg aatagtcgca
     1021 gcgtcacgca cccagcctat tggctggcat gggcacaccc gaaccccggc cggtcaaggg
     1081 ccggcggacg gcgaggctca ggaggcgagc acttcgtcga gcgaggagga ggcgtgctct
     1141 tcgtcggtct tctccgccaa cgcaagctcg gagacgagga tctgccgcgc cttggcaagc
     1201 atgcgcttct cccccgccga gagcccccga tcctggtcgc ggcgccacag gtcgcgcacc
     1261 acctcggaga ccttgatgac gtctccggaa gcgagcttct ccaggttggc tttgtaacgc
     1321 ctggaccagt tcgtcggctc ctcggtgaag ggcgcctgca agacctcgaa aaccttcttg
     1381 agccccttct cgtcgatgac gtcgcgcaca cccacgaggt cgacgttgtc tgccgggacc
     1441 tcgatgacga gatcaccctg agtgaccttc agtttgaggt acagcttctc ctcgcccttg
     1501 atggtcctct tcttgagttc tgtgatcgta gccgcgccgt ggtgcgggta tacgacggtc
     1561 tcgccaacct caaaaagcat agattcttaa tcctttcagc cttgtctatt ctaacacaga
     1621 gcggttgtgg tatagttcgc gccgcacgag ttgtacggcc ggtagaactg aaccggtcgt
     1681 gggttagaat cttctcggtt gtgcgtcatc gggccgtacc aggccacgcg ggaccagtga
     1741 cggaggatct tgtgcgaggt cgtgttgcgg catcggtcat cctggcggcc tgcatcgcca
     1801 tcggcaccgc ggggtgcacc ttcgcgacga accaagacac gaagaacatc aaagagaccg
     1861 ccttcggcgt cagcggcgcg accggtgacg tgagcgtgca taacgcggcg ctcatcaccg
     1921 gcgaaggcag cagcgcgaac ctcgtcgtga ccctcgtcga ctccggcacg aagagccagg
     1981 tcgtcaccat tcagaccccg acgaagaacg gcaagtcgac gcagacggtc acggtgaccc
     2041 cggcgaaccc gcttcagatc ggcggccaag acggttcgca gcgtatccag ttcgacagct
     2101 tcggcgcggc cgcaggcacc ctgcatgacg tgtacttcca gtcgggcgac ggcaacggcg
     2161 tggcgttgaa ggtgcccgtg ttgacgaacc agctgccgac ctacgcgacc ctcacgccga
     2221 cgccgcagcc ggccggcacc ctgcagtaga ggccccctcg ggcgcgagca acgcctcggc
     2281 aggtggccgg cgcgtcgacg tcgaaagacg cgccgatacg aggatcatcc ccgcagccaa
     2341 gaggcgccgg tgcggcctac cttgttggtc ttccaccggc cgagcaacct tgcatgctac
     2401 agctacggca ggcggccggc gagcctcgtc gggctagcga cctcggccgc tctgcgggtc
     2461 gacgaccgcc gcgccgctga gcctcgaggg caacggccct agcaggtgtc cggcggccgc
     2521 agctgcccga cggtctcggg gcgccgtggg tcaggcacgc ccctgggcgg cgacagcggc
     2581 ggcgcctttt gcagccgcct ccgggtcgag gtactcgccg gccccgagcg ggtgaaaatc
     2641 atcatcgagc cgatacacga gcggtatgcc ggtcggaatg ttgagacccg cgatgtcgtc
     2701 gtcgccgatg ccgtcgaggt gcttgacgag cgcccgcaga gagttgccgt gcgccgtgac
     2761 gagcaccgtc ttgccggcag cgaggtcggc ggtgacggca gactcccaat acggcagaag
     2821 ccgcgcgacg acgtctttca ggcactcggt acgcggcagc gcatcgccga gctcccggta
     2881 acgctcgtcg ccggcctgcg aatactccgc gtcgtcgcca agggccgggg ggcgcacatc
     2941 gtacgagcga cgccaggcca tgaactgttc ctgaccgtac tgggccagcg tctgcgcctt
     3001 gtctttgccc tgcagggcgc cgtaatgccg ctcgttgagc cgccacgagc gccgcacggg
     3061 gatccacagg cggtcggcgg cctccaacgc gatgttcgcg gtctggatcg cacggctcaa
     3121 gacggaggtg tgcagcacgt cgggtcgcag gcctgcgccg cgcagcagct cacccgcccg
     3181 ggccgcctct gcggcgccct tctcggtcag ccgcacgtcg acccaaccgg tgaactggtt
     3241 cgacttgttc caggcgctct cgccgtgccg aaggaggatc aacgtgtggc tgctggattc
     3301 catggctcta atctaccggc gaccgcgtgt ccctcccgtc gccagccacg ctcacgcggc
     3361 gacgatctgt tcctgaagcg ccggcacgac cgtctccgcc tcgtcgacgt tcaacggcgc
     3421 acgcaccggc acctcgcgct cgacgacggc gagcgcgacc ggccccagtt cgtgatgcat
     3481 ggcaccggtc gtcacggcgc ccacgacgag gcgctcgccg ccgacctcca gccatacggg
     3541 cgcgccgtgc cgcggcagca ccgactcgga gccgtcgaga tacagcatga cgagtcgccg
     3601 gccggccggc cgggcctcgg ccgcccgcag ggcccgggcg cccggatacc cgaccttgtc
     3661 gcgcggcacg gccgtgcgcg cccaatccaa ctcgcgtggc gtgagagtgc cgtccacctc
     3721 ggtgacctgc ctgggccgcc acgcctcgac gcgtagggcg tcgaaagcga gggcccccgc
     3781 gacgcccggc gcgcccaccc gcccggcgag ggcggcgaga ctctcgcgcg gcacgagtga
     3841 ctcgtgccaa ctccaaccct gggcggggtg cggctctgcc gcggcgtacc gcgggccgcc
     3901 ggcgccggcg ctcggccacg ggtcgtgcca gacgagcggc acgccgtcga cgctcgccgt
     3961 ctcgggcagt tcggcagtga aggtgccaat gacgccgaag tcggcgctgc gatcctcgag
     4021 cgcgacgccg ggttcaccac ccagccggcg ttcgaggtcg ccggcctggc tcgcctcggc
     4081 gatgatccac ggtgcccgac cgtcgtcgat gacgtgcagc gcatggcgga cggcccccga
     4141 cggatcgagc aggagcgtct cggcactgct cgcgcggacg atgtcggccg tgcccagcct
     4201 ctcgaggagc aggcggcccg cgcccgcgga gccgggtgcg ccggccagcc gcaggacgac
     4261 gcgatgcgag aggtcgacga tcgcgccgcg ctccgtcagg cgtcgctgct cccccagcgg
     4321 gttgccgtag tgcgcgggca ctccctcgtc ccatcccgcc ccgagcaccg cccccggcaa
     4381 ccgggagaac gggtcgtcgc ccgcggcgcc cgtcatgccg cggcctggac gcgggccagg
     4441 cgggcggagg cgtgcgtgcg caggtcttgc ccgagcgccg cgatatccca cgcccagagc
     4501 aggtggccgt cgacgagacc gtacatgcgc cgcccggcac tcatcagggc ggcgccaggg
     4561 ccgcgcagca cgccgcctgt cgtaaggtcg atgcg
//
LOCUS       DASTNL010000128         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_462211, whole genome shotgun sequence.
ACCESSION   DASTNL010000128 DASTNL010000000
VERSION     DASTNL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462211"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFQ96_04080"
     CDS             <1..1159
                     /locus_tag="VFQ96_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU0256998.1"
                     /translation="ERALRGTLPVLADEAGAQEAIVATITGYGPLQPYLDDPGVEEIW
                     INRPDRVFVARGGVAERVAVSLTAEQVRDLVERMLQSTGRRVDHSMPFVDASLPDGSR
                     LHVAIADVVRDWSVNIRKFNRSIRDLDTLVRLGALPALAAEFLRASVAVGLNILVCGA
                     TQSGKTTLLNALLSAVGPTERIVTVEETFELDLDVEDVVAMQCRQPSLEGVGEITLRR
                     LIKEALRMRPDRIIVGEVREAESLDLLIALNAGLPGACTLHANGTAEALAKLCTLPLL
                     AGRNISADFIVPTVARSVDLVIFCRMEAGGRRRIVEISATDGPDGRGGVMVRPLFCLV
                     DGELAATGALPGRLEPFRARGIDLHAMLTPAAPAGEGRDALPGEAEIGVPV"
     gene            1156..2019
                     /locus_tag="VFQ96_04085"
     CDS             1156..2019
                     /locus_tag="VFQ96_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0256999.1"
                     /translation="MTLLLACVFAAGVLLMAARFLWPQPRGTVRRPPRRMPLVRLRLN
                     QAGLASVSSGAFLTASAFVAVLAAAVLQASFGVAALSVAAGIGCGVLPFPLLTARAST
                     RREAGRASWPDTVDQIVSAVRSGLSLPDALGRLSRTGPPATRAAFARFEKDYAIGSNF
                     SHSIDLVKDHLADPVGDRILETLRMARQVGGSDLVGVLRDLAASLRSEASVRSELLAR
                     QSWVTSAARLGVAAPWVVLLLLAGRAEGAQAYNSASGIAVILVGLAVCLLAYRVMRAF
                     GRLPEEKRWFG"
     gene            2016..2951
                     /locus_tag="VFQ96_04090"
     CDS             2016..2951
                     /locus_tag="VFQ96_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0257000.1"
                     /translation="MTAHGASWAAILGLLLGLGAWCLLAALPRFRRPSLAARVAPALL
                     DVSPAAAADVAARGGDTALPALLRPLRALAGRLLARLTGGSELLAAKLRAAGQTTSPD
                     EYRTKQVLWLLGGAALGSAAVFVILGVRPIAAPFRLLLPALCALGGLLARDQVLHWSV
                     RARRQRIVDEFPTILDFLALCLSAGEGIRDALERVARAGTGEFSRELAEVAAQTRTGV
                     PLAQALRALGARIGVPALSRCVDAVVAALEHGSPLAGVLRAQANDVREEAKRVLLESA
                     GRKEIAMMIPLVFLILPVTVLFAIYPGVFVLQAGF"
BASE COUNT          427 a         1205 c         1193 g          605 t
ORIGIN      
        1 cgagcgggcg ctgcggggta cgttgccggt gctcgccgac gaggccggcg cacaagaggc
       61 gatcgtcgcg acgatcaccg ggtatgggcc gttgcagccc tacctcgatg atccgggtgt
      121 cgaggagatc tggatcaacc ggcccgatcg cgtcttcgtt gcccgcggtg gcgtcgccga
      181 acgggttgcc gtctcgctga ccgccgagca ggttcgcgac cttgtggagc gaatgctgca
      241 atccaccggt cgacgggtcg atcactccat gccgttcgtc gacgcctctc tacccgacgg
      301 ctcacggctt cacgtcgcga tcgcagacgt ggtgcgcgac tggtcggtca atatccgcaa
      361 gttcaaccgg tcgatccgcg acctcgacac gttggtgcgt ctcggcgcac tgccggcctt
      421 ggcggccgag ttcttgcgcg ccagcgttgc cgtcggcctc aacatcctgg tgtgtggcgc
      481 gacgcagagc ggcaagacga ccttgctcaa tgcgctgctc tccgcggtcg gcccgacgga
      541 acgcatcgtc accgtcgagg agacgttcga actcgatctc gacgtcgagg acgtcgtggc
      601 gatgcagtgc cgccagccca gcctcgaagg ggtgggcgag atcactctgc gccggctcat
      661 caaggaggcg ctgcgtatgc ggcccgatcg gatcatcgtc ggcgaggtgc gtgaggccga
      721 gagcctcgac ctgctcatcg cgctgaacgc cggtctgccc ggggcctgca cactgcacgc
      781 caacggcacc gccgaggcgc tcgccaagct gtgcaccctg ccgcttttgg ccggccgaaa
      841 catctctgcc gacttcatcg tgccgaccgt cgcccgcagc gtcgacctcg tcatcttctg
      901 ccggatggag gccggcggcc ggcggcgcat cgtcgagatc agcgcgacag acggtccaga
      961 tgggcgcggc ggcgtgatgg tgcgtcccct tttttgcctc gtcgacggtg agctcgccgc
     1021 caccggggcg cttccggggc gcctcgaacc gtttcgtgct cgcggcatcg acctgcacgc
     1081 gatgctcacg cccgccgcgc cggcggggga gggccgggat gccctgcccg gcgaggcaga
     1141 gatcggggtg ccggtgtgac cctgcttctc gcctgcgtgt tcgccgccgg cgttctgttg
     1201 atggcggcga ggttcctctg gccgcagccg cgcggaaccg tcagacgccc gccgcgtcgg
     1261 atgcccctcg tacggcttcg gctgaaccag gcaggcctcg cttcggtctc atccggcgcg
     1321 tttctcaccg cctcggcgtt cgtggccgtc cttgccgctg cggtcctcca agcatcgttc
     1381 ggcgtcgcgg ccctctccgt cgccgccggc atcggctgtg gcgtgctgcc gttcccgctc
     1441 ttgacggcgc gggcgagcac gcggcgagaa gccggccggg cgagttggcc tgacacggtc
     1501 gaccagatcg tctctgccgt gcgcagcggc ctcagcctgc ccgacgcgct cggcaggctc
     1561 tcccgcaccg gcccgccggc gacccgtgcc gctttcgcca ggttcgaaaa agactatgcg
     1621 atcggcagca acttctcgca cagcatcgac ctcgtcaagg accacctcgc cgacccggtg
     1681 ggcgaccgga tcctcgagac attgcgcatg gcacggcagg tcggtggcag cgacctcgtc
     1741 ggggtcttgc gcgacttggc cgcctcgctg cgcagcgagg cgagcgtgcg ctccgaactt
     1801 ttggcccgtc aatcctgggt gacgagcgcg gcccggctcg gcgtcgcggc accttgggtg
     1861 gtactgctgc ttctcgccgg ccgggcagaa ggggcgcaag cctacaactc ggcctctggc
     1921 atcgctgtca ttctcgtcgg gctggccgtc tgcctgctgg cctaccgcgt gatgcgtgcg
     1981 tttggccggc tgccggaaga gaaacggtgg ttcggatgac ggctcacggc gcatcctggg
     2041 ctgccattct tggcttgctg ctcggcctgg gcgcctggtg tcttctggcc gcgcttccgc
     2101 ggtttcgtag gccgtcgctc gcggcccgtg tcgctccggc cctcctcgat gtatctccgg
     2161 cagcggccgc cgacgtcgcg gcccgcggcg gcgacactgc ccttcccgcg cttctgaggc
     2221 cgctccgggc gctcgccggg cgcctcctcg cccgcctcac gggcggcagc gagcttctgg
     2281 ccgcgaagct gcgggccgcg ggccagacga cgagtcccga cgagtaccgc acgaagcagg
     2341 tcctctggtt gctcggcggt gcggcgctcg ggtccgccgc cgtgttcgtg atcttgggcg
     2401 ttcgccccat cgcggcgccg ttccgcctcc ttctgccggc gctgtgcgcg ttgggcggcc
     2461 tcctggcgcg cgaccaggtg ctgcactggt cggtgcgggc ccgccgacaa cgcatcgtcg
     2521 acgagtttcc caccattctc gacttcctcg ctctgtgcct gtcggcgggg gaaggcattc
     2581 gcgacgccct cgaacgggtc gcccgcgccg gaacgggtga attctcccga gaactggccg
     2641 aggtcgcggc gcagactcgt accggcgtgc ctctcgccca ggcgttgcgt gcgctgggtg
     2701 cccgtatcgg ggtacctgcg ctcagccggt gcgtggacgc cgtcgtcgcg gcactcgagc
     2761 acggcagccc gctggccggc gtattgcgcg cacaggcgaa cgacgtacgg gaagaggcga
     2821 agcgggtcct cctggaatcg gccggccgca aagagatcgc gatgatgatc ccgctcgtct
     2881 tcctgattct gcccgtcact gtcttgttcg ccatctaccc cggcgtgttc gtcttgcagg
     2941 cagggttctg atgggtggcg cgggaaaccg ccaggccgtg ccgaggtcac ccgatgcctg
     3001 gcgagcacgc gagagagaag aggcaagaag atgaaccaac cgttgtcgca cgatgccgac
     3061 catctgtccg ggggcgcggg ccttgtcgtc gggaggagcc cctcccgaag tccccgctcg
     3121 gtcggcgtcg cgactgcgca cgcagatgcc ggcctgcccg gagacgtccg tcggggcctg
     3181 agaagggggc tgcagacagg gtgggggcgc ctggcgcctc gccgacgaga ccttggggag
     3241 gccttctctg cgcggcggcg tgcgtctcct cgcgtcgacg tgaccaccca tggcatgctc
     3301 cggggcgacc gtggcgacgt tccaggctgg gtcctcgtca cgttgatgac ggccggcctg
     3361 gtactcgtca tctgggcgct cgcaggcccc gcgctgagcg gcgtcttcca gcaggccatc
     3421 gaccgtgtca
//
LOCUS       DASTNL010000129         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_276899, whole genome shotgun sequence.
ACCESSION   DASTNL010000129 DASTNL010000000
VERSION     DASTNL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276899"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..877
                     /locus_tag="VFQ96_04095"
     CDS             143..877
                     /locus_tag="VFQ96_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU0257001.1"
                     /translation="MSEITPTAEDYVKAIWSATEWGEPAITTKGLAARFGTTSATASD
                     TIRRLVLQGLVEHEPYGQIGLTPTGVRHALAMVRRHRLIETFLASTLGYSWAEIHDEA
                     ERLEHAASDLLIDRVDALLDHPAHDPHGDPIPAADGRVSYPQGAVSVRRAEPGDYVIV
                     RISDADAERLVYFHEHGLVPGAAARVVGHDLHASTVRLRVGGSTGAGKAASAELALAG
                     EATDAIILAPAGPAGESAPAPETPTH"
     gene            complement(906..2303)
                     /locus_tag="VFQ96_04100"
     CDS             complement(906..2303)
                     /locus_tag="VFQ96_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257002.1"
                     /translation="MTTPASGERPPATRETRLVLWIAILASFVSFLDGTVVTVALPAI
                     QRSLGGGLTTQQWVVDAYLITLGALILVAGSLSDVFGRVAVLRAGLIGFGVTSLAVAA
                     ASGPVFIIVARCLQGVAGALLVPSSLALIMSTFHGRDEARAIGTWTGATTVAMLIGPV
                     LGGVFVDLVSWRLVFLINVLPIGAALVLLAALRSRDRRVPGSTVDWAGAALCTAGLGG
                     TVFALIEQDARGWADPAIWLPLAVGILCLAAFLLRQARIRQPMMPLVLFRIRNFSAGN
                     LATLFIYGALSLNGLVLGVYLQQGAGLAATLAGLASLPSTVIMIVFSSRSGALAGRFG
                     ARIFMTLGPALMAVGALLLLTVGRDFNYWWQVLPSVVVSGVGLTLTVAPLTAAVLSSI
                     DTARSGIASAVNNAVARVAGLVTVALLGLIAGGSLDLVGFRRAALVTAALMVAGAIAS
                     FLGIRDARTRTSVAV"
BASE COUNT          496 a          909 c         1003 g          307 t
ORIGIN      
        1 gtcggcggct atcgtcgttg tgaacatcct ctcggcggcc aagcccctcc gtccgaggcg
       61 cgggcggcgg atcccgggcg acgagagaac tcacatcgtg cggtgaaaaa gccagacgga
      121 aagcacggtc aggaggccga cgatgagcga gatcacaccg acggccgaag actacgtcaa
      181 ggcgatctgg tcggccacgg agtggggcga gccggcgatc accacgaagg ggctggccgc
      241 gcggttcggc acgacgtcgg cgaccgcctc cgacacgatt cgccgactcg tcttgcaggg
      301 gctcgtcgag cacgagccgt acgggcagat cgggttgacg ccgacgggcg tgcggcacgc
      361 cctggcgatg gtgcgccggc accgtctgat cgagacgttt ctcgcctcga ccttgggcta
      421 cagctgggcg gagatccacg acgaggcgga acggctcgag catgcggcca gcgacctgct
      481 catcgaccgc gtcgacgcac tcctcgacca cccggcacac gacccgcacg gcgacccgat
      541 acccgcggcc gacggtcggg tgagctaccc gcagggggcg gtgagcgtgc gtcgggccga
      601 gccgggcgac tacgtcatcg tgcgcatctc ggatgcggac gccgagcggc tcgtctactt
      661 tcacgaacac ggcctggtgc cgggcgcagc agcccgggtc gtggggcacg acctgcacgc
      721 gagcaccgtg cgcctccggg tcggggggag cacaggcgcc gggaaggccg cgagcgcaga
      781 gctggcgttg gccggcgagg cgaccgacgc catcatcctg gcccccgccg ggcccgccgg
      841 ggagtcggcg ccggcccccg agacacccac gcactgagtc gggcgaagat cggctggccc
      901 gaggctcaga cggcgaccga ggtgcgagtt cgggcgtcgc ggatgccgag gaacgaggcg
      961 atcgcaccgg ccaccatgag ggctgcggtg acgagcgcgg cacgacggaa ccctacgagg
     1021 tcgaggctgc cgcccgcgat gaggccgagc agggccaccg tgacgaggcc tgcgacgcgg
     1081 gcgaccgcgt tgttgaccgc cgaggcgatg ccggagcgtg ccgtgtcgat cgaactgagc
     1141 acggcggcgg tcaacggtgc gaccgtgagg gtgaggccga cgccggagac gacgacggag
     1201 ggcagaacct gccaccaata attgaagtcc cgccccaccg tgagtagcag gagcgcgccg
     1261 accgccatca acgccgggcc gagagtcatg aagatccgcg cgccgaagcg gccggcgagg
     1321 gcgccggaac gtgagctgaa gacgatcatg atcaccgtgg agggcaggct ggcgagcccg
     1381 gcgagtgtgg cggcgaggcc ggcgccttgc tgcagataga cgcccagcac caggccgttc
     1441 agcgagagcg caccgtatat gaaaagggtc gcgaggttgc cggccgaaaa attacggatg
     1501 cgaaagagca cgagcggcat catcggctgc cggatgcgtg cttgtctcag gaggaacgcg
     1561 gcaaggcaga ggatgccgac cgcgagggga agccagatcg ccgggtcggc ccagccccgg
     1621 gcatcctgct cgatgagggc gaagaccgtg ccgccgagac ccgctgtgca cagcgccgca
     1681 cccgcccagt cgacggtgct gcccggcacc cggcgatcgc ggctacgcag ggcggcgaga
     1741 agcacgagtg ccgcaccgat gggcaacacg ttgatgagga agaccagccg ccacgagaca
     1801 aggtcgacga agacgccgcc caagacgggg ccgatgagca tcgcgacggt cgtcgccccc
     1861 gtccacgtgc cgatcgcgcg agcctcgtca cggccatgga acgtcgacat gatgagcgcg
     1921 agggagcttg gcacgaggag cgccccggcc acgccctgca ggcagcgggc cacgatgatg
     1981 aagacggggc cggatgccgc cgcgacggcg agggaggtga cgccgaagcc gatcaggccg
     2041 gcgcgcagca ccgcgacacg gccgaagacg tcgctcagcg agccggcgac gaggatgagc
     2101 gcgccgaggg tgatgagata ggcgtcgacc acccactgtt gcgtggtgag cccacctccg
     2161 aggctgcgct ggatggcggg cagtgcgacc gtcacgaccg tgccgtcgag gaaggagacg
     2221 aacgaggcga ggatcgcgat ccacaagacg agccgagtct cgcgcgtggc cggtggccgc
     2281 tcgccggatg ccggtgtggt catagcacca tcatggccgg agacctgggc cccgtgcgct
     2341 cggatgcgcc tggcgaggct caccgagacc ccttaaaatt agagcggcgt ctgtgacgcc
     2401 agccctgtgt atctacctgg tacccggcca caccgggtcg gccaaggcgg cacccgatcg
     2461 cggcgaacct ggccgcgcga gcaaaggtcg tgaccggtct gaccaccacg cacactccac
     2521 cgccgcacgg cacgccttcg gacgacgaac tcgtcgtgac gggcgacgcc cacccgtccg
     2581 cagcgtcggc agagggcgtc cggccccggg cggaagacat tcggccgacg cagaatcttg
     2641 cgcccgcgag ccggtcttcg gcgacggccg ggcactcatc tgtgcgaagc gacccctacg
     2701 ggcgcccgcc agcgc
//
LOCUS       DASTNL010000130         7251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_834207, whole genome shotgun sequence.
ACCESSION   DASTNL010000130 DASTNL010000000
VERSION     DASTNL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834207"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1818)
                     /gene="sdhA"
                     /locus_tag="VFQ96_04105"
     CDS             complement(7..1818)
                     /gene="sdhA"
                     /locus_tag="VFQ96_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEU0257003.1"
                     /translation="MKPKNDEYAVKDGVYYHKHDIIIIGAGGAGMRAALAALESDPGA
                     DVAVLSKLYPTRSHTGAAQGGMAAALANVEEDNWEWHTYDTVKGGDYLTDQDAAEILA
                     KEAIDAVIDLENMGLPFNRTPEGKIDQRRFGGHTREHGKAAVRRSCYSADRTGHMILQ
                     TLYQNCIRVGVNFFDEFYVLQLLMGEVDGVRRPVAVVAHELATGDLHVFQGKGFIFAT
                     GGFGKMFKTTSNAHTLTGDGVGIVWRNGLPLEDMEFFQFHPTGLYKLGILLTEGARGE
                     GAILRNGEGERFMERYAPTIKDLAPRDIVARSIVKEVQEGRGAGPKKEYVVLDCTHLG
                     AEVLETKLPDITEFARTYLGVDPVTTPVPVMPTAHYAMGGIPTTVNAEVLQNADTIVP
                     GLYAAGECACVSVHGANRLGTNSLLDINVFGKRAGHNIVEYVKTVDYPPLPENPANTV
                     RDMLEGLRENTGTERVAALRQELQEQMDKNAQVFRTDETLANVTKTIHALRERYRNVH
                     VDDKGRRFNTDLLEAVELGFLLDLAEVVVYSARNRKESRGGHMRDDYPKRDDKNYLKH
                     TMAYLTGDPHSSEAGDHITLGWKPVVITNYEPKERKY"
     gene            complement(1865..2305)
                     /locus_tag="VFQ96_04110"
     CDS             complement(1865..2305)
                     /locus_tag="VFQ96_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEU0257004.1"
                     /translation="MTTIETPRTPTRPARKGGVNWDKAGWMFMRGSGVLLLVLIFGHL
                     IVNLVLGQGIKQIDFGFVAGKYASPFWRVWDLLMLWFALIHGSNGMRTIINDYAYSRT
                     VNRALKIAVLVAAIVLIVLGTLVVTTFNPCTGSTPGQFPFCPAS"
     gene            complement(2305..2724)
                     /gene="sdhC"
                     /locus_tag="VFQ96_04115"
     CDS             complement(2305..2724)
                     /gene="sdhC"
                     /locus_tag="VFQ96_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU0257005.1"
                     /translation="MPKQAAGTLEPRTSRPGGTLYRGHEGMWSWVLHRITGVAIFFFL
                     LVHVLDTSLIRVSPDAYNAVIGSYKTVILGFGEIALVGAVVYHGLNGLRVILIDFWRQ
                     GPRFQKPMFWIVVALFVILMAGFIPTQLIHVFSEVSH"
     gene            2913..3557
                     /locus_tag="VFQ96_04120"
     CDS             2913..3557
                     /locus_tag="VFQ96_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEU0257006.1"
                     /translation="MAPKNASRASFRRLVFAVLVAVLPALFVAGPAFAGTRIASAAEP
                     APRPVSWSPAPGSTVSTAVKTITVTFDRDIAGLPGRTGGLQLEVVGPNVGKQHFEAAC
                     PKIENRSVVVPVALGGPGSYRIIYQVVTADGQIASGEGQFDYEPAGPVVAADGAAAPV
                     CVDGRVTAGPSTPATTQSGDDSSAVLLGVVAGAIVLALGGMIVAGRRGRPRRSS"
     gene            3660..4763
                     /locus_tag="VFQ96_04125"
     CDS             3660..4763
                     /locus_tag="VFQ96_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0257007.1"
                     /translation="MSRFYSVIPAGGVGSRLWPLSRADAPKFLHDLSGSGRTLLRDTW
                     DRLVPLSGEQRIMVVTGRAHRAAVEAQLPDLRDHNVVLESEPRDSTAAIGLAAAILER
                     REPGVIVGSFAADHVIRNQTLFQHAVRQAVAVADAGYIGLVGIRPTEPAVGFGYIHAA
                     SPVSIPGAPSALAVDAFEEKPGLATAQEYVDSGDYYWNASMFIGRADLLLAEIERSEP
                     RLAAGIAELAEAWDDPATRGPAVDRIWPTLKKVAIDYTVAEPAAAAGRVAVVPGFFDW
                     NDVGDFASLAKMNSRGGLSDLAILGQGARVLADASSGIVVSQTKRVISLIGVRDIVVV
                     DTPDALLVTTAENAQRVKSVVDALKLSGSADVL"
     gene            complement(4795..5745)
                     /locus_tag="VFQ96_04130"
     CDS             complement(4795..5745)
                     /locus_tag="VFQ96_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU0257008.1"
                     /translation="MPTKMILDCDTGIDDAMAIMYAALHPSIDLLAVGTVWGNVPVSM
                     ATRNTVHVLGLAGRTAIPVAQGAAGPLLGGPVEYAFAVHGDDGQGGAYDGTDHGPSVP
                     ISAARQIVETVRANPHEVWLVPVGPLTNIALALALEPALPRLVKGVSLMGGAALAPGN
                     FSPVAEANIAHDPEAAAAVFRAPWPIVMAGLDVTMKILLTPAHRATLAAGGAAGAYLA
                     RVLGFYGDFYAATAFDTWSAAIHDPIAVAAAAGTLGIRRAPTVRVEVDCTAGPSRGAT
                     VCDLRGRHPGTPAQEGAHCTVLLDADDTIASDIVALIAAA"
     gene            complement(5730..6797)
                     /locus_tag="VFQ96_04135"
     CDS             complement(5730..6797)
                     /locus_tag="VFQ96_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257009.1"
                     /translation="MTERERTRRATRTDVARLAGTSVAVVSYVVNGGPRPVAATTRAR
                     VLEAIDAVGYQPNTIARALASGVTSLYGLILPSISNPFFAALAHELEDAIAATGKILL
                     LGDSSGSKEREAELVGVLARRRTDGVFFIGVDDSPDLDALAGTPTVLLDRYSPAKAGQ
                     YSVTIDNDEAAHAATRHLIEHGYTRLAIIAGPPDLAVSRARLDGWRRALHDAGITPDE
                     RWVVAAPFSAGGGFEAGRTLVTSGAPPRAVFASSEQQSLGLLAAAAEAGLRVPEDLAI
                     MSFDGTEASEYAVPRISGIVQRLPDIAAAAVETLEALCARREPPARHVTTGYELRIRH
                     SCGPHETPTHPDREAIPCRRR"
     gene            complement(6794..>7251)
                     /locus_tag="VFQ96_04140"
     CDS             complement(6794..>7251)
                     /locus_tag="VFQ96_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0257010.1"
                     /translation="VSQSTLRAADPLIVNEHEARALLHASAGADASATAPGAAMGIPQ
                     ALAAARTLAEGFAASAVVTLGQSGAVAAAGGDAWHEPAPKTDDVVDSTGAGDAFVGAL
                     AACLAFGANLADAVRCGVAAGTAATGAAGTVASYGRLPRLHPTTPAGTP"
BASE COUNT         1019 a         2440 c         2616 g         1176 t
ORIGIN      
        1 tgggcatcag tacttcctct ccttgggctc gtagttcgtg atgaccacgg gcttccaacc
       61 tagtgtgatg tgatcccccg cctcgctgga gtgtggatcc ccggtgaggt acgccatggt
      121 gtgcttcaga tagttcttgt cgtcgcgctt cgggtagtcg tcgcgcatgt ggccgccgcg
      181 actctctttg cggttgcggg ccgagtagac gaccacttcg gccaggtcga gcaggaagcc
      241 cagctcgacc gcctcgagca ggtcggtgtt gaaccggcgg cccttgtcgt cgacgtgcac
      301 gttgcggtac cgctcgcgca gcgcgtggat ggtcttcgtg acgttcgcca aggtctcgtc
      361 ggtgcggaac acctgcgcgt tcttgtccat ctgctcttgc agctcttgcc gcaacgcggc
      421 gacccgctcg gtgcccgtat tctcacgcag gccctcgagc atgtcgcgca cggtgttcgc
      481 cgggttctcc ggcagcggcg ggtagtcgac ggtcttcacg tattcgacga tgttgtggcc
      541 ggcgcgcttg ccgaacacgt tgatgtcgag cagtgagttc gtgcccagcc ggttcgcgcc
      601 gtgcacagag acgcaggcgc attcgccggc cgcgtacagg cccggaacga tcgtgtcggc
      661 gttctgcaac acctcggcgt tcaccgtcgt ggggatgccg cccatcgcgt agtgtgcggt
      721 cggcatgacc ggcaccggtg tcgtgaccgg gtcgacgccg agataggtgc gggcgaactc
      781 ggtgatgtcg ggcagcttcg tctcgagcac ctcggcgccg aggtgggtgc agtcgagaac
      841 gacgtattcc ttcttcgggc cggcgccccg gccctcttgc acctctttca cgatcgaacg
      901 ggcgacgatg tcgcgcggcg cgaggtcttt gatcgtcggc gcgtaacgct ccatgaaccg
      961 ttcgccctcg ccgttgcgca ggatcgcccc ctcgccgcgc gccccctcgg tcaagaggat
     1021 gccgagcttg tacagcccgg tcgggtggaa ctggaagaac tccatgtctt ctagaggcag
     1081 cccgttacgc cagacgatgc cgacgccgtc acccgtcaag gtatgcgcgt tcgaggtggt
     1141 cttgaacatc ttgccgaagc cgccggtggc gaagatgaag cccttgccct ggaaaacgtg
     1201 caggtcgccc gtcgccagct cgtgggcgac gaccgcgacg gggcggcgca cgccgtcgac
     1261 ctcgcccatc aagagctgca agacgtagaa ctcgtcgaag aagttcacgc ccacgcggat
     1321 gcagttctgg tacagcgtct gcaagatcat gtggcctgtg cggtcggcgg agtagcacga
     1381 acggcgcacg gccgccttgc cgtgctcccg cgtgtggccg ccgaaacgcc gctggtcgat
     1441 cttgccctcc ggcgtgcggt tgaacggcag gcccatgttc tcgaggtcga tgacggcgtc
     1501 gatcgcctct ttggcgagga tctcggccgc gtcttggtcg gtgaggtagt cgccaccctt
     1561 gacggtgtcg taggtgtgcc actcccagtt gtcttcttcg acgttcgcga gcgctgccgc
     1621 catgccgccc tgggccgcac cggtgtgcga cctcgtcggg tagagcttgg agagcactgc
     1681 gacgtcggcg cctgggtcgg attcgagggc ggccagtgcc gcacgcattc cagccccacc
     1741 ggcgccgatg atgatgatgt cgtgtttgtg gtagtagacc ccgtctttga cggcgtactc
     1801 gtcgttcttc ggtttcacgt ctggcgcttt ctgtcgttac tgggcggtca tcgggtcggg
     1861 ccgctcacga ggccgggcag aacgggaact ggcctggtgt ggagccggtg cacgggttga
     1921 acgtggtgac cacgagggtt ccgagcacga tgagcacgat ggcggccacc aagacggcga
     1981 tcttcaacgc gcggttgacg gtgcgtgaat acgcgtagtc gttgatgatc gtgcgcatgc
     2041 cgttcgagcc gtggatcagc gcgaaccaca gcatgagcag atcccacacc cgccagaacg
     2101 gcgaggcgta cttgccggcc acgaagccga agtcgatctg cttgatgccc tggccgagca
     2161 ccaggttgac gatgaggtgg ccgaagatga gcaccaagag cagcacgccg gagccgcgca
     2221 tgaacatcca gccggccttg tcccagttga cgccgccctt gcgtgccggg cgggtggggg
     2281 tgcgcggcgt ctcgatcgtc gtcatcagtg actcacctcg ctgaacacgt ggatcaattg
     2341 ggtcgggatg aagcctgcca tgaggatgac gaagagcgcc acgacgatcc agaacatcgg
     2401 cttctggaac cggggcccct gtcgccagaa gtcgatgagg atgacgcgca ggccgttcag
     2461 gccgtgatag acgaccgcgc cgacgagggc gatctcgccg aagccgagga tcaccgtctt
     2521 atagctgccg atgaccgcgt tataggcgtc tggactcaca cggatgagcg aggtgtcgag
     2581 cacgtggacg aggagaaaga agaagatcgc cacgcccgtg atccgatgca gtacccacga
     2641 ccacatcccc tcgtggcctc gataaagcgt gcctccgggc cgggaagtcc gcggctccag
     2701 ggttcctgcc gcttgttttg gcacgaacaa ccctccttgg ctgtgaatcc ggatggtctt
     2761 gcggccgcct tggcggcggt cgccccaagt ctatgacgtg gccacgacgt agcggagcgc
     2821 ccggactgcc cgcgggggcc gcgccggcgg ccgacccgct cgcggcgagg acgattctgg
     2881 ccggtcccgg cacactcgcg taccctttcc tcgtggcccc caagaacgcc tcccgcgcct
     2941 cgtttcgtcg cctcgtcttc gcggtgctcg tcgccgtgct gccggcactc ttcgtcgccg
     3001 gcccggcgtt cgccggcacg cggatcgcgt cggccgccga gcccgcgcca cggcctgtct
     3061 cgtggtcacc ggcgcccggc tccaccgtct ccacggcggt gaagacgatc actgtcacct
     3121 tcgacaggga catcgcgggc ctgcccgggc gcaccggcgg ccttcagctc gaggtcgtcg
     3181 gcccgaacgt cggcaagcag catttcgagg ccgcctgccc gaagatcgag aaccgctccg
     3241 tcgtcgtgcc cgttgcgttg ggcgggccgg gcagctaccg cattatttat caggtcgtta
     3301 ccgccgacgg ccagattgcc tccggagaag ggcagtttga ctacgaaccg gcggggcccg
     3361 tcgtcgccgc cgacggcgcg gcggcgcccg tctgcgtcga cggccgcgtg acggccggcc
     3421 cgtcgacgcc ggccacgacg cagtcggggg acgactcgtc ggcggtcttg ctgggggtcg
     3481 tagcgggcgc catcgttctt gcgttgggcg ggatgatcgt cgccgggcgc cgcggccggc
     3541 cgcgccgatc ctcctgagcc cgcccgcacg caggcatctg gtggttggct gggcgcgtcg
     3601 tgtggcgcct gggcgcaaaa cggcggcgtg gatattctga gcgggtggcc acgagtgcga
     3661 tgagtcgttt ctatagcgtc atcccggccg gcggcgtcgg ctcccggctg tggccactct
     3721 cccgggcgga cgcgccgaag tttctgcacg acctgagcgg cagcggccgc acgctcttgc
     3781 gtgacacgtg ggatcgcctg gtaccgctct cgggcgagca acgcatcatg gtcgtgacgg
     3841 gccgcgcgca tcgggcggcg gtcgaagcgc agctgcccga cctgcgtgac cacaacgtgg
     3901 tgttagagag cgagccgagg gattccaccg ccgccatcgg gctggccgcg gcgatcttgg
     3961 agcggcgcga gcccggcgtc atcgtgggct ccttcgccgc cgaccacgtc atccgcaacc
     4021 agaccttgtt ccagcacgcc gtacggcagg ccgtcgcggt cgccgacgcc ggctacatcg
     4081 gcctcgtcgg gatccgcccc accgagccgg cggtcgggtt cggttacatc cacgccgcct
     4141 cgcccgtctc gataccgggc gcgccgagcg cgctggccgt cgacgccttc gaggagaagc
     4201 cgggccttgc gaccgcgcaa gagtacgtcg acagcggcga ctactactgg aacgccagca
     4261 tgttcatcgg ccgtgccgac cttctcctcg cggagatcga gcgcagcgag ccgcggctgg
     4321 ccgcgggcat cgcggagctg gccgaggcgt gggacgaccc ggccacccgg gggcccgccg
     4381 tcgatcgcat ctggccgacc ttgaaaaagg tcgccatcga ctacacggtg gcggagccgg
     4441 ctgccgcggc cggccgggtc gccgtcgtgc ccggattctt cgactggaac gacgtgggcg
     4501 atttcgcctc gctggcgaag atgaactccc gcggcgggct ctccgacctc gcgatcctcg
     4561 gtcagggcgc ccgcgtgttg gcagacgcct ccagcggcat cgtcgtcagc cagacgaaga
     4621 gggtcatcag cctcatcggc gtgcgagaca tcgtcgtcgt cgacacgccc gacgcgctgc
     4681 tcgtcaccac cgccgagaac gcgcagcggg tgaagtcggt ggtcgacgcg ttgaagctct
     4741 cgggctccgc cgacgtgctc tgagccgagt gtgcggcatc cggccgcggc ctcgtcaggc
     4801 ggcggcgatg agagccacga tgtcggaggc gatcgtgtcg tcggcgtcga gaagcaccgt
     4861 gcagtgcgcg ccttcttgcg ccggcgtgcc gggatgccga ccgcgcaggt cgcagacggt
     4921 ggcgccgcgc gacggcccgg cggtgcagtc cacctccaca cgcacggtgg gggcccggcg
     4981 gatgcccaac gtgccggccg ccgccgcgac ggcgatcggg tcgtggatgg ccgccgacca
     5041 cgtgtcgaac gcggtggccg cgtagaagtc gccgtagaag cccagaacgc gggccaggta
     5101 ggcgccggcc gcgccgccgg cggcgagcgt cgcgcgatgc gccggagtga ggaggatctt
     5161 catcgtcacg tcgaggcccg ccatgacgat cggccacggc gcacggaaga ccgcggccgc
     5221 cgcctccggg tcgtgggcga tgttcgcctc ggcgaccggt gagaagttgc cgggcgcaag
     5281 cgccgcgccg cccatcagcg agacgccctt cacgagccgg gggagagccg gctccagggc
     5341 cagggcgaga gcgatgttcg tcaacgggcc gaccggcacg agccacacct cgtgcggatt
     5401 cgcccgcacc gtctcgacga tctgccgtgc cgccgagatc ggcacggacg ggccgtggtc
     5461 ggtgccgtcg taggccccgc cctggccgtc gtcgccgtgc accgcgaacg catactcgac
     5521 gggcccgccg agaaggggcc cggccgcgcc ctgcgccacc gggatggctg tgcggccggc
     5581 gagccccaag acgtgcacgg tgttgcgggt ggccatcgag accggcacgt tgccccagac
     5641 cgtgcctacg gccaggaggt cgatggacgg atgcagcgcg gcatacatga tcgccatcgc
     5701 gtcgtcgatt ccggtgtcgc agtcgagaat catcttcgtc ggcatgggat cgcctcgcgg
     5761 tcggggtggg tgggggtctc gtgcgggccg cacgagtgcc ggatgcgcag ctcgtagccg
     5821 gtcgtcacgt gccgggccgg cggctccctg cgcgcgcaca gcgcttccag ggtctcgacc
     5881 gcggcggcgg cgatgtcggg cagcctctgc acgatgcccg agatgcgggg caccgcgtat
     5941 tccgaggcct cggtgccgtc gaacgacatg atggcgaggt cttccggcac gcgcaggcct
     6001 gcttcggccg cagcggcgag aagcccgagg ctctgctgtt cgctggaggc gaagacggcc
     6061 ctgggtggcg cgcccgaggt cacgagcgtg cggcccgcct cgaagccgcc gccggccgag
     6121 aagggcgccg ccacgaccca gcgttcgtcg ggcgtgatgc ccgcgtcgtg gagcgctcgc
     6181 cgccaaccgt cgagccgggc gcggctgacg gccaggtcgg gagggccggc gatgatcgcg
     6241 aggcgtgtgt agccgtgctc gatcaagtgc cgggtggcgg cgtgggcggc ctcgtcgttg
     6301 tcgatcgtga ccgaatactg ccccgccttc gccgggctgt accggtcgag cagcaccgtc
     6361 ggtgttccgg cgagcgcgtc gaggtcgggt gagtcgtcga cgccgatgaa gaacacgccg
     6421 tcggtgcgcc gacgggcgag cacgccgacg agctccgcct cgcgttcctt gctgccggag
     6481 gagtcgccga gcagcagaat ctttcccgtc gccgcgatcg cgtcttcgag ttcgtgcgcg
     6541 agcgccgcga agaacggatt cgagatggac ggcaggatca ggccgtaaag gctcgtcacg
     6601 ccggaggcca gggcgcgggc gatggtgttc ggctggtagc cgaccgcgtc gatcgcctcg
     6661 agcacgcggg cccgggtagt ggccgcgacg ggccgcgggc cgccgttgac gacgtagctg
     6721 acgacggcga cggaggtgcc ggccaggcgc gcgacatccg tgcgggtggc ccttcgggtg
     6781 cgctcccgct cggtcatggc gtgccggccg gtgtcgtggg gtgcagacgc ggcaggcggc
     6841 cgtatgaggc gaccgtgccg gccgccccgg tcgcggcggt tccggcggcg acgccgcacc
     6901 gcaccgcgtc ggcgaggttc gccccgaagg ccaggcaggc ggcgagcgcg ccgacgaacg
     6961 cgtccccggc gcccgtcgag tcgacgacgt cgtcggtctt cggcgcgggc tcgtgccatg
     7021 cgtcgccgcc cgccgcggcg acggccccgg actggccgag ggtcacgacc gccgaggcgg
     7081 cgaagccttc ggcgagcgtg cgggcggccg ccagggcttg tgggatgccc atcgcggcgc
     7141 ccggtgccgt ggcgctcgcg tcggcgccgg cgctcgcgtg gagaagggcc cgggcctcgt
     7201 gctcgttgac gatgagcggg tcggcggcgc gcagcgtcga ctggctgacc g
//
LOCUS       DASTNL010000131         8129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_93144, whole genome shotgun sequence.
ACCESSION   DASTNL010000131 DASTNL010000000
VERSION     DASTNL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93144"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="pth"
                     /locus_tag="VFQ96_04145"
     CDS             complement(<1..527)
                     /gene="pth"
                     /locus_tag="VFQ96_04145"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU0257011.1"
                     /translation="MKNTWLVAGLGNPGPRYAGTRHNAGRATVAELARREGLRFSVHK
                     AQALVAEGAPGADGRFVFAQPNTFMNSSGGPLAQLMRFYDVSPDRLIVVHDDLDLPFG
                     TLRLKFGGGHGGHNGVRDIIARLGTPEFTRVRIGIGRPGTDGAVIDFVLQRFTAVQRR
                     QVDQVFADAADAVEM"
     gene            complement(547..1173)
                     /locus_tag="VFQ96_04150"
     CDS             complement(547..1173)
                     /locus_tag="VFQ96_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEU0257012.1"
                     /translation="MSNDENNDEKIVARRRTEFGKGFARRLRVAGLIPAVIYGHKSQP
                     QHIAVPAHQTSLLLRKANAVLRLEVDGVDQLALVKDVQRNPVKQTIEHLDLIVVRRGE
                     KVRVEVPVHLDGETFPGTIPMIELQTLQLEVEATQIPEHVVVAVGGAEEGTQVFAGAI
                     PLPAGAVLLDDPKELVVNVVVPRASAEDIAADEAAEAAAEGAPAAAAE"
     gene            complement(1439..1963)
                     /locus_tag="VFQ96_04155"
     CDS             complement(1439..1963)
                     /locus_tag="VFQ96_04155"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEU0257013.1"
                     /translation="MAERSNDVPPLVVVMGVSGSGKSTVGEALAGRLGVRFVDGDDLH
                     PASNVAKMAAGHPLTDDDRWPWLARIGGALADARETGLVVACSALKKSYRDVILAKEP
                     RSFFVHLAGPRRVLESRLGARHGHFMPPSLLDSQLATLEPLRDEPGAAVDISGDVGTV
                     VDAALAALARHAAR"
     gene            complement(2047..3276)
                     /gene="manD"
                     /locus_tag="VFQ96_04160"
     CDS             complement(2047..3276)
                     /gene="manD"
                     /locus_tag="VFQ96_04160"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEU0257014.1"
                     /translation="MTIERAEVLVTSPGRNFVTLRLTTSDGTTGLGDATLNGRELAVA
                     EYLREHVAPLLTGLDPTRIEDTWQYLYRGAYWRRGPVTMAAIAAVDMALWDIKAKTAG
                     MPLYQLLGGRSRRGALAYGHASGSDLPSLFQSIRNHLAEGFRAVRVQTSVPGLGQVYG
                     VATDQRPGQRYDYEPAGRGARPTEETWDTRAYLRHVPTVFRAVREEFGPDLPLLHDGH
                     HRMTPNEAAVLGKSLEPYDLFWLEDCTPAENQEALRRVRSQTTTPLAIGEIFNTVFDY
                     QSLITEQLIDYVRSSVTHAGGVTPMKKLMDFAAVYQIKSGFHGPTDVSPVGQAAALHL
                     DLAIHNFGIQEYMKHSEDTLRVFRTSYTFEDGLLNPGEKPGLGVEYDDEAAATFPYAP
                     AYLPVNRLQRDGSMHDW"
     gene            complement(3396..4817)
                     /locus_tag="VFQ96_04165"
     CDS             complement(3396..4817)
                     /locus_tag="VFQ96_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257015.1"
                     /translation="MSTTKATLTMEQSEVERLADQANDVLLKEKGTKVSTYGGWMMIA
                     TILVEAWDLYAMSFVLIFIKAEYKPNALEIGWLAGGVQAGALLGAILGGWLADRLGRR
                     RLFLLTMLLFVILALIQAFSVNVWDLITMRILIGVPLGADIAVGYTYIMESMSRGARD
                     KMGSRWQGMFGLGEVLSIVVVTIMYASGIDHAVLWRVALGIGAVPAFILLLARINLPE
                     TPMALILSGKFVEAKKVARNLFDEPLDMIPDQDALVTKPKVGDFLKVIWADRTKRRAS
                     IFAWISNACQGAEFTAFGFYIPVILVTAGVGVASGGNLIGTNLVTAAIFVVATISGFL
                     APVMIAKTGHRGVARWGFGLAFIGLLLGALGLGANIAWVIIIGAAVLMWGHYWDASNG
                     MTIASMVAPARFKGTASGFGYIFVKGASFFGAFVFPLLTAAWGPVGATLAVSVLSLVG
                     FLAAQFILPEVYRYIENEKVQTA"
     gene            5035..5793
                     /locus_tag="VFQ96_04170"
     CDS             5035..5793
                     /locus_tag="VFQ96_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0257016.1"
                     /translation="MTTEQRRGPSQAQHERILDALGEAIVAGRLAAGERLLAAEIAER
                     YRASRSTVREAVRVLESMGMAQSRRNVGVEILPAERWNSFSPRLIEWRLAAADRLSFL
                     HSLSELRSAIEPLAARLAAQQASAQQCAALVMAAKGMREAASDADGPTYLDHDMRFHE
                     TLLESSHNPLLLGLKGVVAEVLRGRTRHALMPHDADPSAVRLHQAVAARVSEGDAAGA
                     EDAMRAIIAEADTATARLGTTQRRESITSRKDPS"
     gene            5790..7331
                     /locus_tag="VFQ96_04175"
     CDS             5790..7331
                     /locus_tag="VFQ96_04175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEU0257017.1"
                     /translation="MTTRLSPENTPDGSLIGAAAPAETGIVHLGLGNFHRAHVAVYTA
                     LALQREEGPWGIAGFANRSHRVVDAMRRQGLRYSVLRLSDERRGAGVVAVHRAARVAA
                     EDRGAFVAAVADPDCRILTLTISELGYARSPQTNRLDTAQPDVRADLADPTRPHSTIG
                     MLALALARRSEGGLPLSVLSCDNLQGNGDVTRAMLTEYAALSGLGPDVQRFLATQVTF
                     PNSMVDRIVPATTAAMSDEVERLLGVRDACPVPAEEFSMWVFEDDFAAGRPAWQLAGA
                     IPSDEVHAYELVKLRLLNGSHSLMAYLGALEGCETIADCWAEASIREAVLTGIRLDYL
                     PTITVPTGFDADDYVASLGRRFANRAIAHRTAQVATDGSAKLQQRIPDAAAFALDRGR
                     MPHMLALTVAAWICAAVPPDGFTAGPIAARMREPARDLLAAVTRGAHSPAEHARAVMR
                     GGFFPDLLTRHEAFVDRVAGLVTTIARHGARAAAAEATAAAAHEARPAEPASPPNVTT
                     PRDEQ"
     gene            7399..>8129
                     /locus_tag="VFQ96_04180"
     CDS             7399..>8129
                     /locus_tag="VFQ96_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0257018.1"
                     /translation="MKALAIHGKGEIRWEERPIPEPGEGEVRLRVAYVGICGSDLHYY
                     FHGANGEYAVREPLTPGHELSARVDLDPSGTLAAGTPVTVHPARYGHPVPGLAHSPHL
                     WPGGDYLGSAATLPHRQGGGAEYLVVEAGMIRVLPGGLPLTRAALAEPLAVALHAVRR
                     AGGVQGATVLVNGAGPIGLLTLAAALASGASSVDAADIREEPLERARTLGAGATWLST
                     SVETGPPAEGYDVVFECSGAAAGLTT"
BASE COUNT         1293 a         2851 c         2795 g         1190 t
ORIGIN      
        1 atcatctcga ccgcatccgc cgcgtcggcg aacacctggt cgacctggcg acgttgcacc
       61 gccgtgaagc gttgcagcac gaagtcgatg acggccccgt cggtgccggg ccggccgatg
      121 ccgatgcgca ccctggtgaa ctccggcgtg ccgaggcgcg cgatgatgtc ccgcaccccg
      181 ttgtggccgc cgtgtccgcc gccgaacttc agacgcagcg ttccgaacgg caggtcgagg
      241 tcgtcgtgca cgacgatgag ccggtcgggc gacacgtcgt agaagcgcat cagctgggcg
      301 agcggcccgc ccgaggagtt catgaacgtg ttcggctggg cgaagacgaa acgcccgtcc
      361 gcccccggcg caccttccgc gaccaaggcc tgcgccttgt gcacggaaaa gcgcaggcct
      421 tcgcggcgag ccaactccgc cacggtcgcc cggccggcgt tatgccgggt cccggcgtag
      481 cgcgggccag ggttgccgag gcccgcgacg agccaggtgt tcttcattgc gcccggtgcc
      541 ggcgcgctat tcggcagccg cagccggcgc gccctcggcc gccgcctcgg cggcctcgtc
      601 ggccgcgatg tcctcggcag aggcgcgcgg cacgacgacg ttgacgacga gttccttcgg
      661 gtcgtcgagg agcaccgcgc cggccggcag cgggatcgcc ccggcgaaga cctgcgtgcc
      721 ctcttcggcg ccgccgaccg ccacgacgac gtgctccggg atctgggtcg cctcgacctc
      781 cagctgcagg gtctgcagct cgatcatcgg gatcgtgccg gggaacgtct cgccgtcgag
      841 atgcacgggc acctcgacgc gaaccttctc gccgcgccgc acgacgatga ggtcgaggtg
      901 ctcgatggtc tgcttcacgg ggttgcgctg cacgtctttg acgagcgcca gctggtcgac
      961 gccgtcgacc tcgagtcgga gcaccgcgtt cgccttgcgc agcagcaggg aggtctgatg
     1021 cgccggcacc gcgatatgtt gcggctgcga cttgtggcca tagatcacgg ccgggatgag
     1081 gcccgccacc cgcagccgac gggcgaagcc cttgccgaac tcggtgcgtc ggcgggcgac
     1141 gatcttctcg tcgttgttct cgtcgttgct cataactctc cctcgcaggc cggtcgggcc
     1201 cagaatcgat tctcttgttc cactcgaacg catgacagcg tgaggacaag gcctgccagc
     1261 ctgctccaga cgcgtcgatc acggatgcct acggcatccc tcgccgatgt ccggtcatca
     1321 atcttacagg aggcggcgcc cggctcacgc acaggcggcc ggcgcaccca gcccatggcg
     1381 ccggccggtc atcccccacc acggtgccgg ccgcgtggcc gagagccctg ggtcggcgct
     1441 acctcgcggc gtgacgggcc agggcggcca gggccgcgtc gaccacggtg ccgacgtcgc
     1501 cggagatgtc gacggctgca cccggctcgt cgcgcaacgg ctccaacgtg gccagctggg
     1561 agtcgagaag agagggaggc atgaaatggc cgtgccttgc gcccaggcgg ctttccaaca
     1621 cgcggcgcgg cccggccagg tgcacgaaga agctgcgcgg ttctttcgcg aggatcacgt
     1681 cgcgatacga cttcttgagc gcggaacacg ccacgacgag cccggtctcg cgggcgtcgg
     1741 cgagcgcccc gccgatcctc gccagccagg gccagcggtc gtcgtcggtc agcgggtgac
     1801 ctgccgccat cttcgcgacg ttagaggccg gatgcaggtc gtcgccgtcg acgaagcgca
     1861 cgccgagtcg gccggccaac gcctcgccca cggtgctctt gcccgatccg gagacgccca
     1921 tgacgacgac gaggggcggc acgtcattcg agcgttcagc catggcttcc gaatctagcg
     1981 cagcggcgcg gggccggccg agcccgcggg cccgcaggtc ggcgacggcc gcaggctccc
     2041 gccgaatcac cagtcatgca tggagccgtc acgctgcagc ctgttcaccg ggaggtaggc
     2101 cggggcgtac gggaacgtcg ccgccgcctc gtcatcgtat tcgacaccga gcccgggctt
     2161 ctctccgggg ttgaggagcc cgtcttcgaa ggtgtaactc gtgcggaaga cacgcagggt
     2221 gtcctccgaa tgcttcatat actcctggat gccgaagttg tggatcgcga ggtcgaggtg
     2281 caaggccgcc gcctgcccga ccggcgagac gtcggtgggg ccgtggaagc ccgacttgat
     2341 ctggtagacc gccgcgaagt ccatgagctt cttcatgggc gtcacgccgc cggcatgcgt
     2401 gacggaggaa cgcacgtagt cgatcagctg ctcggtgatg aggctctggt agtcgaagac
     2461 ggtgttgaag atctcgccga tcgccagcgg cgtcgtcgtc tgcgatcgga cgcgccgcaa
     2521 cgcctcctga ttctcggcgg gcgtgcagtc ttctagccag aagaggtcgt agggctccag
     2581 ggacttgccg agaacagccg cctcgttggg cgtcatgcga tggtgcccgt cgtgcaggag
     2641 cggcaggtcg ggcccgaact cctcccgcac cgcccgaaac acggtgggca cgtgccgcag
     2701 gtaggcacgg gtgtcccacg tctcttctgt gggccgcgcg cccctgccgg ccggctcgta
     2761 gtcgtagcgc tggccgggcc gttggtcggt ggcgactccg tagacctggc cgaggccggg
     2821 aacactcgtc tgcacccgca cggcgcggaa tccctcggcg aggtggttgc gaatcgactg
     2881 gaagagcgac ggaaggtcgg aaccggaggc gtgcccatag gcaagcgcgc cgcggcgcga
     2941 ccggccgccc agcaactggt agaggggcat gccggccgtc ttggctttga tgtcccacag
     3001 ggccatatcg accgcggcga tcgccgccat cgtcacgggg ccgcggcgcc agtaggcgcc
     3061 ccgatagagg tactgccagg tgtcttcgat gcgggtcggg tcgaggcccg taagaagcgg
     3121 tgcgacgtgc tcgcgcaggt attcggcgac ggcgagctca cggccgttca acgtcgcatc
     3181 ccccagcccg gtcgtaccgt cggaggtcgt cagccgcagc gtgacgaagt tgcggccggg
     3241 actcgtcacc aagacctcgg cccgttcgat ggtcaccatg gaggctcccc catccctttc
     3301 tcgatcgttc gcccgggcgc ctgggcggca ccgcgggccg cgccgcggcc gacccccggc
     3361 ccgccccggc tctgctggcc ggacccggct ttcggctagg ccgtctggac cttctcattc
     3421 tcgatgtagc ggtagacctc cggcaggatg aactgtgccg cgaggaagcc gacgagcgag
     3481 aggaccgaca ccgcgagagt cgcgccgacg gggccccagg ccgcggtcag aagcgggaag
     3541 acgaacgcgc cgaagaagga cgcgcccttc acgaagatgt agccgaagcc cgacgcggtt
     3601 cccttgaacc gggcgggggc gaccatcgag gcgatggtca tgccgttgga ggcgtcccaa
     3661 tagtgacccc acatgagcac ggcggcgccg atgatgatga cccaggcgat gttcgcgccg
     3721 agccccagag cgcccaacag caacccgatg aacgccaggc cgaaccccca gcgggccacc
     3781 ccgcggtggc cggtcttggc gatcatgacg ggcgccagga atccggagat cgtggcgacg
     3841 acgaagatgg cggccgtgac gaggttggtg ccgatgaggt tcccgccgga ggcgacgccg
     3901 acgcccgcgg tgacgaggat cacgggaatg tagaaaccga acgccgtgaa ctcggcacct
     3961 tggcaggcgt tggagatcca cgcgaagatc gacgcgcgac gcttcgtgcg gtcggcccag
     4021 atcaccttga ggaagtcgcc gaccttcggc ttggtgacga gggcatcctg gtcggggatc
     4081 atgtcgagcg gctcatcgaa gaggttccgc gcgaccttct tggcctccac gaactttccg
     4141 gagaggatga gcgccatcgg cgtctcgggg aggttgatgc gcgccagcag caggatgaag
     4201 gcaggcaccg caccgatgcc cagcgccacc cgccagagga ccgcgtggtc gatgccgctg
     4261 gcgtacatga tcgtgacgac gacgatggag agcacttcgc cgagcccgaa catgccctgc
     4321 caacggctgc ccatcttgtc gcgggcaccg cgcgacatcg actccatgat gtacgtgtag
     4381 ccgaccgcta tgtcggcgcc gagcggaacg ccgatcagaa tgcgcatcgt gatgagatcc
     4441 caaacgttca ccgagaacgc ctgaatcagg gcgaggatga cgaagagcag catcgtcaga
     4501 aggaagagtc ggcgccgccc gagccggtcg gcgagccagc cgccgaggat cgccccgaga
     4561 agggcgccgg cctgtacccc gccggcgagc cagccgatct cgagggcgtt cggcttgtat
     4621 tccgccttga tgaagatcaa gacgaacgac atcgcgtaga gatcccacgc ctcgacgagg
     4681 atcgtggcga tcatcatcca gcccccgtag gtgctgactt tggtgccctt ctctttcaag
     4741 agcacgtcgt tggcctggtc ggccagacgt tcgacctcgg actgctccat tgtgagagtc
     4801 gccttcgtgg tgctcatcgc ccggcctccg cccatccggc ggcaccggcg cggtcccgct
     4861 gaagaagaga tccgcggtac gcagtcattc ctgacctcat cgtcgctcct agatcgagtt
     4921 tcaaactgta gagaagccta tacgtatgtt ttatcatgtc aatacctgac atatgtcggg
     4981 tgccatgctt cgcctcacac gctacagtgc ggatacgtac ggaatactcg ggtcatgacc
     5041 acggaacagc gacgcggacc gtcacaagcg caacacgagc gcatcctgga cgccctgggt
     5101 gaggcgatcg tcgccggtcg tctcgcggcg ggcgagcgcc tcttggccgc ggagatcgcc
     5161 gagcgctacc gggcctcacg ctcgaccgtg cgcgaagccg tgcgcgtgct cgaatccatg
     5221 gggatggcgc agtcgcgccg caacgtcggg gtggagatcc tgccggccga acggtggaac
     5281 tccttctcgc cccggctcat cgagtggcgg ctggcggccg ccgaccgact gtcattcctc
     5341 cactcgctca gcgagctgcg ttcggccatc gaaccgctcg ccgcacgcct ggccgcacag
     5401 caggcgagcg cccagcaatg cgctgcgctc gtcatggcgg ccaaggggat gagagaggcc
     5461 gcctccgacg ccgacggccc gacctacctc gaccacgaca tgcgttttca cgaaactctc
     5521 ttggagtcct cgcacaaccc gctgctcctc ggcctgaagg gcgtcgtcgc cgaggttctg
     5581 cgcggccgca cccggcacgc gttgatgccg cacgacgccg acccttctgc cgtgcgactc
     5641 caccaagccg tcgccgcccg cgtctcggag ggagacgccg ccggcgccga ggacgccatg
     5701 cgcgccatca tcgcggaggc cgacacggcg acggcccggc tgggcacgac acagcgacga
     5761 gagtccatca caagtcgaaa ggacccgtca tgacgacgcg cctctccccc gaaaacaccc
     5821 ccgacggctc gctgatcggc gccgcggcgc cggccgagac cggcatcgtt catctcgggc
     5881 tgggcaactt tcaccgcgcg cacgtcgccg tctacacagc ccttgccctt cagagagagg
     5941 aaggcccctg gggcatcgcg ggcttcgcga accgctccca ccgggtcgtc gacgcgatgc
     6001 gccggcaagg cctgcgatac agcgtcttgc gtctttccga cgagcgccgc ggcgcgggcg
     6061 tcgtcgccgt gcaccgagcc gcacgggtgg cggccgaaga ccgcggcgcc ttcgtcgcgg
     6121 ccgtcgcgga ccctgactgt cgcatcctca ccctgacgat cagcgagctg ggctatgcca
     6181 ggtcgccgca gacgaaccgg ctcgacacgg cgcagcccga cgtgcgcgcc gacctcgccg
     6241 atccgacgcg gccgcacagc acgatcggca tgctcgccct ggcactcgcc cggcgatcgg
     6301 agggcggcct gccgctcagc gtcctctcct gcgacaactt gcagggcaac ggagacgtca
     6361 cgcgggcgat gctcaccgag tacgcggcgc tttccgggct ggggcccgac gtccagcgct
     6421 tcctcgccac ccaggtcacc ttccccaact ccatggtgga ccggatcgtg ccggcgacga
     6481 cggccgcgat gagcgacgag gtcgaacgcc tcctcggggt gcgggatgcc tgcccggtgc
     6541 cggcagagga gttctcgatg tgggtcttcg aagacgactt cgcggcgggc aggccggcct
     6601 ggcagctggc cggcgcaata ccgagcgacg aggtgcacgc ctacgagctc gtcaagctgc
     6661 gcctcctgaa cgggtcacac tcgctcatgg cctatctcgg cgccctcgaa ggctgcgaga
     6721 cgatcgccga ctgctgggca gaggcatcca tccgtgaggc ggtcttgacg ggcatccgcc
     6781 tcgactactt gcccaccatc accgtgccga ccgggtttga cgccgacgac tacgtcgcct
     6841 cgctggggcg gcgcttcgcg aaccgggcga tcgcccaccg caccgcgcaa gtcgcgaccg
     6901 acggctcggc aaagcttcag caacgcattc ccgacgccgc ggccttcgcc ctcgacaggg
     6961 ggcgcatgcc ccacatgctc gccctgacgg tggcggcgtg gatctgcgcc gccgtgccgc
     7021 ccgacggctt cacggcgggg ccgatcgccg cccggatgcg ggagcccgca cgcgatcttt
     7081 tggccgcggt gacacgcggc gcacacagcc cggccgagca tgccagggcg gtcatgcggg
     7141 gcgggttctt ccccgacctg ctcacccgcc acgaggcttt cgtcgaccgg gtggcggggc
     7201 tcgtcacgac catcgcacga cacggcgccc gggcggcggc cgccgaggct accgccgccg
     7261 ccgcacacga ggcccggccc gcggagcccg caagcccgcc gaacgtgaca acgccgcgag
     7321 acgagcagtg aggcagcaca aaggacgaag gatgcgccgg gcacgccggg acgcgcatgc
     7381 gagaagaagg gacgagcgat gaaggcactg gcgatccacg ggaagggcga gatccggtgg
     7441 gaggaacggc ccatccctga gccaggtgag ggcgaggtgc ggctgcgcgt cgcctacgtc
     7501 ggcatctgcg gctccgacct gcactattac ttccacggcg cgaacggcga gtacgcggtg
     7561 cgggagcctc tgacacccgg ccacgagctc tcggcgagag tcgacctcga cccctccggc
     7621 accctcgccg cgggaacccc cgtcacggtg catccggccc ggtacggcca ccccgtgccg
     7681 gggctcgcgc attcgccgca cctgtggccc ggcggggact atctgggcag cgccgccacg
     7741 ctgccgcacc gtcaaggcgg cggcgccgaa tacctcgtcg tcgaggccgg gatgatccgg
     7801 gtgctgcccg gcggcctgcc gctcacccgc gcggcgctcg cggaacccct ggcggtcgcg
     7861 ctgcacgcgg tgcgccgcgc cggcggggtg cagggcgcga cggtgctcgt gaacggcgcc
     7921 ggcccgatag gcctcctcac gctcgcggcc gccctcgcgt cgggggcgtc gagcgtggac
     7981 gccgccgaca ttcgcgagga accgctcgag cgggcacgca ccctgggggc gggcgcgacg
     8041 tggctgtcga ccagtgtcga gacgggcccc cctgccgagg ggtatgacgt ggtcttcgaa
     8101 tgctcgggtg ctgcggcggg gctcacgac
//
LOCUS       DASTNL010000132         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1574948, whole genome shotgun sequence.
ACCESSION   DASTNL010000132 DASTNL010000000
VERSION     DASTNL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574948"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFQ96_04185"
     CDS             <1..258
                     /locus_tag="VFQ96_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEU0257019.1"
                     /translation="VPWFLVGFLAVAILNSTGIIAPGLRAFLVQASIFLIATALAGIG
                     LSTDVAGLRRAGWRPLLLGAILWILVACTSLAVIRLTGGLG"
     gene            465..1028
                     /locus_tag="VFQ96_04190"
     CDS             465..1028
                     /locus_tag="VFQ96_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257020.1"
                     /translation="MRARVRDTLPQDGRLGMARRISRQVFESLAQGDYALDDYLAIED
                     TQERDRAIAGTVERMLASLPQESRYNQLIGPFYDTPGLERWSGKTRQAIEKWRRARKV
                     LSITTSTGRILYPSFQFGPKAELLPGLPGVIQTLTEQAPNPLTQARWLNTPTTRFNGH
                     TAAELLRDGHIESVLAAARADAARLAG"
     gene            1025..1717
                     /locus_tag="VFQ96_04195"
     CDS             1025..1717
                     /locus_tag="VFQ96_04195"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU0257021.1"
                     /translation="MTRGTPALRPPTTDLHSFPSTERKTASSHLYRSHRAGRAPWHFD
                     DTLGPAGGRFNLPAPRGTCYAADDLATAVREGLGSVLGQTNVIEEDLAVSRVVSRIAV
                     AGTLHVAELNNPNAVRHGANRELSASTDYPTTQKWAATLADAGFGALHYESRFTTKAA
                     PTAWALFGPLPETMMLWDSRHDVPGHQACRRCGIEILPAMLDDASARVIDPPGDTTAA
                     GRQPRCRWPAGS"
     gene            complement(1714..3270)
                     /locus_tag="VFQ96_04200"
     CDS             complement(1714..3270)
                     /locus_tag="VFQ96_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0257022.1"
                     /translation="MPDARRDPQARGRRGIRALAVFATLAAATLLTACVGPATRNAPS
                     MRSTPTGEHVSAALAPYYHQTLLWAPCGTDLQCTHASVPLDWAHPAGAHISLALIRHP
                     ATGQSDGDLLVNPGGPGGSGVDFVRDSLASAVDATVERHFTVIGFDPRGVGASTPVKC
                     LPPSGLDHYLYGITPGTPGSATWITASIKEATTFADACRKNTGPLLAHVDTASAARDM
                     DVLRAALGQKKLDYLGYSYGTYLGTVYAGLFPGKVGRFVLDGALDPASTNFDVTLEQA
                     KGFERALTAYLTACIGTKGCPFTSVTEGLGEVRRLLAQVTAHPLHSGGRALGANTLVT
                     AIIYPLYDANAWSLLNRLFTQVRRGDAAFAFTLADAYNERQSDGSYKDNSAEAFTAIN
                     CLDYHYDDNVAHMRAQAARLEKAAPVIGRYLAYGDISCAVWPYRSTVHRGPIHAAGAA
                     PIVVVGTTGDPATPYAWAKALASQLDSGRLITYRGQGHTAYNKSNACVDDAVDDYLTR
                     GTLPRRGLTC"
     gene            complement(3318..>4466)
                     /locus_tag="VFQ96_04205"
     CDS             complement(3318..>4466)
                     /locus_tag="VFQ96_04205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU0257023.1"
                     /translation="EAVETLAAAALGGGALTHAWLITGPPGSGRSNLAFAFAAALLSP
                     DGRGEDEATLNQVRARTHPDLDVLTTTGVIIRRDDVREMVQRSHYAPSTGPYRVIVVE
                     DADRMTERTSNFLLKELEEPAERTIWVLCAPSEADMLPTIRSRVRVVRLRTPSVEAVA
                     RLLVERDGVEQGAALRAARQAQSHIGMAHRLATDPPASERRQRTVEAVLAVHTAPDAV
                     AAAAELLEIAKEDADAVTAQLDTEEREHALRALGVEPGARIPPALRGQVRRLEEAQKR
                     RATRGLRDGLDRILVDLLSVFRDVGALQLGAGVPLVNAEAEEALRAWAVRARPQGVLA
                     VLDAVAQARGRLAGNVAPALAVEALLVTVAAAAGGHIPWPSGLAGAAR"
BASE COUNT          662 a         1557 c         1601 g          646 t
ORIGIN      
        1 gtgccctggt ttctcgtggg gttcctcgcc gtggcgattc tcaactcgac cggcatcatc
       61 gcgcccggcc tgcgggcgtt cctcgtgcag gccagcatct ttctcatcgc cacggcgctg
      121 gccggcatcg gcctctccac cgacgtcgcg gggctgcgcc gggcgggctg gcggccgctt
      181 ctgctgggcg cgatcctctg gatcctcgtc gcctgcacgt cgctcgccgt catccggctc
      241 accggggggc tcggatgagc gaggcggagt acgaccagcg gcaacgcggt cttcgcctcg
      301 cccaccggtg ctcgaccgac cgcgccaggc tcgaccagga cgacttcgcc caagcccggt
      361 cgggcccgca cgctccccac gtcacgcgac gaagcagtac ggttgacgca gttgacgaca
      421 ctcgatacga tgggagaagc cgacagccga gaggaggccc gaccatgcgc gctagagttc
      481 gggacaccct gccgcaagac ggccgcctgg gtatggcacg ccggatcagc cgacaagttt
      541 tcgaatcact cgctcagggt gactacgccc tcgacgacta tctggctatc gaagacacgc
      601 aagagcgaga ccgggcaata gccggaacgg tcgagcggat gctcgcctcc ctcccccaag
      661 aaagccgata caaccagctg atcgggccgt tttatgacac gcccggccta gagaggtgga
      721 gtgggaagac ccgccaggcc atcgagaagt ggcgtcgcgc ccgaaaggtg ctctcgatca
      781 ccacgagcac ggggcgcatt ctctacccgt cgttccagtt cggccccaag gcggagctgc
      841 tacccggcct ccccggcgtc atccagacac tcaccgagca ggcaccaaat ccgctgacgc
      901 aagcccggtg gctgaacacc ccgacaacga ggttcaacgg gcacacggcg gcagaactgc
      961 tccgcgacgg ccacatcgaa tccgttctcg ccgcggcccg cgccgacgcc gcccggctgg
     1021 ccgggtgact cgcggcaccc cggccttgcg gccgcccacg acagacctcc actcgtttcc
     1081 ctctaccgaa cgcaagaccg cgtcgagcca cctctaccgt tcccaccggg cgggccgcgc
     1141 tccgtggcac tttgacgaca cgctcgggcc ggccggcggg cgattcaacc tgccggcgcc
     1201 acgcggaacg tgttacgcgg cggatgacct ggccaccgcg gtgcgtgagg gcctcggctc
     1261 ggttctcggc cagacgaacg tgatcgagga ggacctggcg gtctcccgtg tcgtatcccg
     1321 catcgccgta gcgggcacac tacacgtggc agaactgaac aatcccaatg cggtacgaca
     1381 cggcgcgaac cgcgaactct ccgccagcac cgattacccc accacccaga aatgggctgc
     1441 gactcttgcc gacgccgggt tcggggccct tcactacgaa tcccggttca cgacgaaggc
     1501 cgcaccgacc gcgtgggccc tcttcggccc gctgcccgag acaatgatgc tgtgggattc
     1561 acgccacgat gttcccggac accaggcgtg ccgaaggtgc ggcatcgaga ttcttcccgc
     1621 gatgctcgac gacgcatccg cccgcgttat cgatccgccg ggcgacacca cggccgccgg
     1681 caggcaaccg cggtgccggt ggccggcagg gtcttagcag gtgagacccc ggcgcggcaa
     1741 cgtgccgcgg gtgaggtagt cgtcgacggc gtcgtcgacg cacgcgttcg acttgttgta
     1801 ggcggtgtgg ccctggccgc ggtaggtgat gaggcggccg ctgtcgagct ggctcgccaa
     1861 cgccttcgcc cacgcgtagg gggtggccgg gtcgcccgtc gtgccgacga cgacgatcgg
     1921 ggcagcgccg gccgcgtgga tcgggccgcg gtgcaccgtg ctccggtaag gccagactgc
     1981 gcagctgatg tcgccgtagg cgaggtaacg gccgatgacc ggcgcggcct tctccagcct
     2041 cgccgcctgc gcccgcatgt gggcgacgtt gtcgtcgtag tggtagtcga ggcagttgat
     2101 cgccgtgaag gcctcggccg aattgtcctt atagctgccg tcgctctgcc gctcgttgta
     2161 ggcgtcggcc agggtgaacg cgaatgccgc gtcgccgcgg cgcacctgcg tgaacaggcg
     2221 gttcaagagc gaccaggcgt tcgcgtcgta gagcgggtag atgatcgccg tcacgagcgt
     2281 gttggcgccg agcgcgcggc cgccactgtg cagggggtgc gcggtgacct gggcgagaag
     2341 ccgccgcacc tcgcccaggc cctcggtgac gctcgtgaac gggcagccct tcgtgccgat
     2401 acaggcggtg agataggcgg tcagcgcgcg ctcgaagcct ttcgcctgtt ccaacgtcac
     2461 gtcgaagttc gtcgaggccg ggtcgagcgc gccatccaac acgaaccggc ccaccttgcc
     2521 ggggaagagg ccggcgtaga cggtgccgag gtaggtgccg taggagtagc cgagatagtc
     2581 gagcttcttc tggccgaggg cggcgcgcag tacatccatg tcgcgtgccg cgctggccgt
     2641 gtcgacgtgg gcgaggagcg gcccggtgtt cttacggcac gcgtcggcaa acgtggtggc
     2701 ctctttgatc gacgccgtga tccacgtggc gctgcccggc gtgccgggcg tgatgccgta
     2761 gaggtagtgg tcgaggccgg agggcggcag gcatttcacc ggggtggagg cgccgacgcc
     2821 gcgcgggtcg aagccgatca cggtgaaatg gcgctccacc gtcgcgtcga ccgcgctggc
     2881 caggctgtcg cgcacgaagt cgaccccgga gccgcccggg cctcccgggt tcacgagcaa
     2941 gtcgccgtcg ctctgccccg tcgccgggtg ccggatcaac gcgagagata tgtgggcgcc
     3001 ggcggggtgc gcccagtcga gcggcacgct tgcgtgcgtg cattgcaagt cggtgccgca
     3061 cggcgcccag aggagcgtct ggtgatagta gggggcgagc gcggcggaga cgtgttcgcc
     3121 ggtgggcgtg gaacgcatcg acggcgcgtt ccgggtcgcc gggccgacac acgccgtgag
     3181 cagggtcgct gcggcgagcg tggcgaagac cgcgagggcg cggatgccgc gacgcccgcg
     3241 ggcctgcggg tcgcgacggg catccggcat gcgactccga tggagcctca acggagaacg
     3301 ccacggcatc cggaacgtca ccgggcggcc cccgccaggc cggacggcca ggggatgtgc
     3361 ccgcccgccg cagcggcgac cgtgacgagc agcgcctcca ccgcgagcgc cggggcgacg
     3421 ttgccggcca ggcggcccct ggcctgcgcc accgcgtcga gcacggcgag caccccctgc
     3481 gggcgggcac gcacggccca ggcacgcagg gcctcctccg cctccgcgtt gacgagtggc
     3541 acgccggcgc ccaactgcag ggcgccgacg tcacgaaaga cggagagcag gtcgacgagg
     3601 atgcggtcga gcccgtcgcg cagcccgcgc gtcgcccggc gcttctgagc ctcttcgagg
     3661 cggcgcacct gcccgcgcaa cgccggcggg atgcgcgcgc cgggctccac ccccaacgcg
     3721 cgcagcgcgt gctcgcgttc ctcggtgtcg agttgggcgg tgacggcgtc ggcgtcttct
     3781 ttggcgatct cgagaagctc ggcggcggcc gcgaccgcgt cgggcgcggt gtgcacggcg
     3841 agaaccgcct cgacggtgcg ctgccggcgt tcgctcgccg gcgggtcggt ggcgagccgg
     3901 tgggccatgc cgatgtggct ctgtgcctgc cgggcggcgc gcagggccgc cccctgctcc
     3961 accccgtcac gttccacgag aagccgggcg accgcctcga cggagggcgt gcgcagccgc
     4021 acgacccgca cccgggagcg gatcgtgggc agcatgtcgg cctcgctcgg cgcgcacaac
     4081 acccagatgg tgcgctcggc cggctcttcg agctctttca agagaaagtt cgaggtgcgc
     4141 tccgtcatcc ggtctgcgtc ttcgacgacg atgacacggt aggggccggt ggagggcgcg
     4201 tagtgggagc gttgcaccat ctcgcgcacg tcgtcgcggc ggatgatgac cccggtcgtc
     4261 gtcaagacgt cgaggtcggg gtgtgtgcgg gcccgcacct ggttcagggt cgcctcgtct
     4321 tcgccgcggc catcgggtga gaggagcgcg gccgcgaacg cgaacgccag gttggatcgg
     4381 ccggagccgg gcggcccggt gatcagccac gcgtgggtga gcgcgccgcc gcccagcgcc
     4441 gccgccgcga gggtttcgac cgcctc
//
LOCUS       DASTNL010000133         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1019114, whole genome shotgun sequence.
ACCESSION   DASTNL010000133 DASTNL010000000
VERSION     DASTNL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019114"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFQ96_04210"
     CDS             <1..199
                     /locus_tag="VFQ96_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HEU0257024.1"
                     /translation="NPDYAIRTFFLGPPDDALLHGLLDVPGWQQLSFSAAAQAVQNSA
                     YPALYARWEEPARVWLAALSR"
     gene            280..1287
                     /locus_tag="VFQ96_04215"
     CDS             280..1287
                     /locus_tag="VFQ96_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0257025.1"
                     /translation="MTQKARWGILATGGIAHSFAGDLVLDGHTIAAVGSRHRDSADAF
                     AAQYGIPHAYGSYEELVADPDVDIVYVATPHPFHARDAALALRAGKHVLVEKPITLNA
                     AEAQGLVDLAAERGLLILEAMWTRFLPHVVRIREIIAEGTLGTLRSFTADHRQKLPDD
                     PAHRINAPELGGGALLDLGIYPVSFAAHLFGSPRDVLATAVFGPTGVDAQVATLFRYA
                     DGQIATTLSAAHSRGPNTASIVGTDARIDIDGVWYTPTTFRVTDASGTVLETWQNQVP
                     GRGMQYQAREAERLLAAGKTASEILSPAESVSIMTTMDEIRRQIGLAYPSEKAAGAVA
                     R"
     gene            1334..>2724
                     /locus_tag="VFQ96_04220"
     CDS             1334..>2724
                     /locus_tag="VFQ96_04220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0257026.1"
                     /translation="MSETGDRGNETPPGGQQPGDGIFAALGRRKTGWVIAAASAAFVL
                     LGGGAYAFGSALGGAAAPGAPVATSTAQPAAKTARPVPASPAAAEPVRTCSVQTLADD
                     PALGTLQAQVRDARTGTVLFDRDGDTPHAAASVMKIVTGAAALAALGPDFRLTTTVVA
                     GSTPGSVVLVGGGDSTLTRLPDGIRSYYDGAAHLDDLASQAVEAMGGTPITAVYVDTS
                     LYGGPAWQPTWNAHTERIVDGSSDPVTALQVDGGRANPQAQNSTRTSDPVTQAANAFI
                     TALDARGANVPQVADASTIEKTAPAAPATSAPTPAPGGHEASKGGDGHDAGHGHGGGP
                     MPGTVLATVQSQPVSNLLGQALTDSDNTIMETLTRQVALKEGTGNTFAAENAAVLKAL
                     TKYGIATTGLHVADGSGLSVDNRVPPSYLTQLLAQIQAGKNGLGVIAQALPVAGQTGT
                     LGPGYGRFTGRSAV"
BASE COUNT          414 a         1081 c          887 g          342 t
ORIGIN      
        1 gaaccccgac tatgccatcc gcacgttctt tctcggcccg cccgacgacg ccctgctcca
       61 cggccttctc gacgtgcccg gctggcagca gctgtcgttc tcggccgcgg cgcaggccgt
      121 gcagaactcc gcctaccccg cgctctacgc ccgctgggag gaaccggccc gcgtctggct
      181 cgccgcactc agccgctgag ccgcaccacc tacggccgtg ccgggggtgg tcggtaggct
      241 gagaagccga gcatgacgcc aacgaaggga tgccggcaga tgacgcaaaa ggcacgatgg
      301 ggaatccttg ccactggagg catcgcgcac tccttcgcgg gtgacctcgt tctcgacggc
      361 cacacgatcg ccgcagtcgg ctcccggcac cgcgactcgg ccgacgcgtt cgccgcccag
      421 tacggcatac cgcacgccta cggcagctac gaagagctcg tcgccgaccc cgacgtcgac
      481 atcgtctacg tggcgacgcc gcacccgttc cacgcccgcg acgcggcgct cgcgctgcgg
      541 gccggcaaac acgtgctcgt cgagaagccc atcacgctga acgcggccga ggcgcagggg
      601 ctcgtcgacc tcgccgccga acgcggcctg ctcatcctcg aagccatgtg gacgaggttc
      661 ctgccgcacg tcgtgcgcat ccgcgagatc atcgccgagg gcaccctcgg cacgctgcgc
      721 tccttcaccg ccgaccaccg ccagaaactg cccgacgacc cggcgcaccg catcaacgcg
      781 cccgagctgg gcggcggggc gctgctcgac ctcggcatct acccggtctc gttcgccgcc
      841 cacctcttcg gcagcccgcg cgatgttctc gccaccgcgg tcttcggccc caccggggtc
      901 gacgcgcagg tggccacctt gttccggtac gccgacggcc agatcgccac cacgctctcg
      961 gccgcccatt cccgcggccc caacacggcg tcgatcgtcg gcaccgacgc gcgcatcgac
     1021 atcgacggcg tttggtacac gccaacgacc ttccgcgtca ccgacgcctc tggcaccgtg
     1081 ctcgagacgt ggcagaacca ggtgcccggc cgcggcatgc agtaccaggc ccgcgaggcg
     1141 gaacggcttc ttgccgccgg caagaccgcc agcgagattc tctcacccgc cgaatccgtc
     1201 tcgatcatga caaccatgga cgagatacgg cggcagatcg gcctcgccta cccgtcggag
     1261 aaggctgccg gcgccgtcgc gcgatgacgg cagcctccag ccggcgttca cgaggcgccg
     1321 ttaggatcgc cgggtgagcg agaccggcga tcgggggaac gagacgccgc ccggggggca
     1381 gcagcctggc gacggcatct tcgccgcgtt gggcaggcgc aagaccggct gggtcatcgc
     1441 ggccgcctct gccgcgttcg tgttgctcgg cggcggggca tacgcgttcg gttccgccct
     1501 gggcggcgcg gcggcccctg gcgctcccgt cgcgacatcg acggcgcagc ccgcggccaa
     1561 gacggcccgc cccgtgcccg cctcacccgc cgccgccgag cccgtgcgca cctgctccgt
     1621 tcagaccctc gccgacgacc cggcgctcgg caccctgcag gcccaggtgc gcgacgcgcg
     1681 caccggcacc gtgctcttcg accgtgacgg cgacacgccg cacgccgccg cgagcgtcat
     1741 gaagatcgtc acgggcgcgg ccgcgctcgc cgccctcggg cccgactttc gcctgacgac
     1801 gaccgtcgtc gcgggcagca cacccggctc cgtcgtgctc gtcggcggcg gcgactccac
     1861 cttgacgagg ctgcccgacg gcatccgctc ctactacgac ggcgccgccc acctcgacga
     1921 cctcgcgagc caggcggtcg aagcgatggg cggcacgccg atcaccgccg tctacgtcga
     1981 cacgtcgctc tacggcgggc cggcatggca gcccacctgg aacgcgcaca ccgaacgcat
     2041 cgtcgacggc tccagcgacc cggtcacggc gctgcaggtg gatggcggcc gcgcgaaccc
     2101 gcaggcacag aactccaccc gcacgagcga cccggtcacg caagccgcga acgcgttcat
     2161 cacggcgctc gacgcgcgcg gtgcgaacgt gccgcaggtg gccgatgcct ccaccatcga
     2221 gaagacggcc ccggccgcgc ccgccacgtc ggcccccacc ccggccccgg gcggccacga
     2281 ggcctcgaaa ggcggcgacg ggcacgacgc ggggcacggc cacggcggtg ggcctatgcc
     2341 cggcacggtg ctcgccaccg tgcagtcgca gcccgtctcc aacctgctgg gccaggcctt
     2401 gaccgactcc gacaacacga tcatggagac cctgacccgc caggtcgcct tgaaagaggg
     2461 caccggcaac acgttcgcgg ccgagaacgc cgccgtgttg aaggcgctga cgaaatacgg
     2521 catcgccacg accggcctgc acgtcgccga cggctcgggg ctgagcgtcg acaaccgggt
     2581 gccgccctcg tacctcacgc agcttctcgc gcagatccag gccggaaaga acgggctcgg
     2641 cgtcatcgcg caggcgctgc ccgtcgccgg gcaaaccggc acgctcggcc ccggttacgg
     2701 ccggttcacg ggccgcagtg cggt
//
LOCUS       DASTNL010000134         8764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1206997, whole genome shotgun sequence.
ACCESSION   DASTNL010000134 DASTNL010000000
VERSION     DASTNL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206997"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..706)
                     /locus_tag="VFQ96_04225"
     CDS             complement(155..706)
                     /locus_tag="VFQ96_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257027.1"
                     /translation="MVVGVFALVLEVVGAVTLAALAVALLVTIVRSALWLMESRPLPG
                     SVSGIRFEAEAARLDKDEFTQDWWVIPVVMVNVSHRPLRVPVMWRIATVWTGRRVGVL
                     RRRYSYRGEFRLDVTSLPQHERYMNPGETVVGAVTVSLPADQSPVRLSVAKDDGRPRP
                     GIRLTGPVHTIGLIRDPGRTHVS"
     gene            complement(850..1962)
                     /gene="moaA"
                     /locus_tag="VFQ96_04230"
     CDS             complement(850..1962)
                     /gene="moaA"
                     /locus_tag="VFQ96_04230"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU0257028.1"
                     /translation="MRTTMDPETKAARASLTDGFGREHTDMRISLTDKCNFRCVYCMR
                     EDQRFNLTRLELDTPELVRVARIAHNLGIRTIRLTGGEPLMRKDAIDVVRQLSTIGFD
                     DISMTTNGSLLTRKVGGITAAQALREAGLNRLNISCDSLQPDKFAKIRRRGDFDVVMA
                     GIAEAHRVGFSPIKMNTVVIRGQNEDEILDFANYVRESWEAGTPISVRFIEFMPLGET
                     GEKQENWTKDKVVRSADIVKMIGDQWPLKISSSQKDHAPASRWAFQDGKGEIGVIPTM
                     TNPFCANCNRLRITGDGFVMRCLFSRKDSKYALRNLMRRDGGCTDEEIAQAFLAAVAS
                     KRAGQDRDTPDIFENPGKYVGGSQAVHQVSMSQIGG"
     gene            2151..3341
                     /locus_tag="VFQ96_04235"
     CDS             2151..3341
                     /locus_tag="VFQ96_04235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0257029.1"
                     /translation="MSAPPHVSDEETPARSAASRTVTVIVPTRNEAGNVGPLVARLAT
                     ALSGVAAEVLFVDDSDDDTPRAVLHAARRGGLPVRLIHRTHGAGGLSGAVVAGLTASR
                     ARWCVVMDGDLQHPPELIPALLARGEAEEAEVVVASRHRAGGSSSGLDGWLRHAVSRA
                     STLVTRGMFPRRLHGCTDPMSGFFAVRRASLNLGDMRPRGFKVLLEILTHHRFRIAEE
                     PFVFGVRSSGSSKAGAGNGLQFLVQLAVLRFGRLSKFGMIGAFGAVMNLAIMAWLMWA
                     GTGYLVAAIVGGAVTIVMNFVLQEWLVFKDLRHGRHGFAGRFARSVGFDTAETAVRTG
                     VLWLLVRFLGFHSILAQALLLCVGFLARFVYRSLVVYRQDTAEPLTLLPDGLPVPEEA
                     APGD"
     gene            complement(3398..5149)
                     /locus_tag="VFQ96_04240"
     CDS             complement(3398..5149)
                     /locus_tag="VFQ96_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257030.1"
                     /translation="MVAAEANTLAGGASLGRVSVADAWRARRAAQLGMCLFVGDIRGD
                     LVRVTMARVRGRVGRRPAAPKGRRWVWWLIVAVIVATAFTARLLYLLHTGGLLQARNY
                     DDGVYYAATDAFVHGRLPYRDFLLLQPPGVIFAAAPAAWLGSLVGDPVGLITGRLLFI
                     AAGAVNAGLVAVILRRFGYAAAAFGGFFYALDFMAIYTERSLLLEPFGTLCILVGLAL
                     QGRVSHSDAPPGTKAAGVDRAGVVSTGWASWLRAPERWLVIAGIVLGFSTSFRIWNIV
                     PAVVIVLFTRGARRRVRLLVGVVVGALVCYLPFFVFAPSTMFREIVTDQMGRPRVHQS
                     ILHKLHIILGDAITPGFGLSTRKLIYVLLVLAILAVVVALAVRDSRLYVALFFSGLAV
                     LLLSPSFFPHYTALTNPALALILGVAFGRLCALLRPRAGRVALTVVAIAGILVVYLPA
                     ELRGAQPAPPARGLQAAAASVDGCVLADDPTILAVMNVLSRDLKAGCPLWPDVTGWTY
                     DADKVTEDGVAVARAKNAVWQKAAVDYLLSGSAVIQYRAGTGLDAASAAEIRRGRVLF
                     HDGIWTLYATPQPAVER"
     gene            5232..6455
                     /locus_tag="VFQ96_04245"
     CDS             5232..6455
                     /locus_tag="VFQ96_04245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0257031.1"
                     /translation="MALRTLDQSAKLKNVLYEIRGAALAEAERLEADGQHILKLNTGN
                     PAAFGFEAPHQIVRDMIAAIPEAHGYSQSRGILSARRAVVARYEETPGFPHVDPDDVY
                     LGNGVSELITMVTQALLDDGDEVLIPSPDYPLWTAMTSLAGGVAVHYRCDPDDAWQPD
                     LEDIRAKITDRTKAIVVINPNNPTGAVYGRQSLEGIVEIAREHSLLLLADEIYDRILY
                     DDAEHVNLASLAPDLLCLTFNGLSKTYRVAGYRSGWLVITGPRRHAAGFLHGLGLLAS
                     TRLCPNVPAQYAVQAALSGVQSIDTLLVPGGRLHDQRDVAWQAVTSIPGVSCAKPRGA
                     LYLFPRLDPEVYDIHDDARFVYDLLVSEHVLLVQGTGFNWPTSDRFRIVTLPEARVLS
                     NAIERIGNFLASYRQ"
     gene            complement(6506..8686)
                     /locus_tag="VFQ96_04250"
     CDS             complement(6506..8686)
                     /locus_tag="VFQ96_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0257032.1"
                     /translation="MGLPSLTEHGLLLMWVQLLVILVVARALGSLMQRIGQPRVIGEL
                     LAGVLLGPSVLAHLWPAAERFLVPHSLASAAPINAIGWIGVAFLLVLTGFETDLTLVR
                     KLGRPATFVAVGGLAVPFAAGLAVGFWMPSSLMGEHSTATAFVLFIAVSLSISSLPVI
                     AKILSELGLMRRDFAQVTVAVGMVNDLVGWLALGIIAALGRTSHVTFGTIAYPLAAIA
                     VILAIAIFVGQRGVDAVLRLVRKRDRTGADAMGMTLLITFLFAVATQLARSDAVLGAY
                     IAGILIGRSRFFQERTRRQLQSVNAAIFAPVFFATAGLRVNLASLADPTTLAWAGIIL
                     AVAVGSKFGGAFGAARLARLGGRESLALAVGLNARGAVEVVIATVGLTIGVLSGSVYT
                     AIVVMAIVTSMMAPPLLRLIVRGWQGSAEERLRLQREAAMESNLLIRRGRLLMPTRGG
                     PNSLAVARVLHDAWPPESPVTLLAIGTDAGKRPDLREILQVFENRDVEVRQVRGAALD
                     ATLAEARLGYVALGLGSRDSFREGRILNPVVDDLLSISPVPMVIVRGGRGDSRPAAGE
                     PFRKVLVPVTGSTGSRAAQEFGYGIAQRTGADLVLAHIVTQPERDSGREVSAEAPSAR
                     QAEAASQAAVSVVREALSHAREQNVPARGRVRAGRVRGEEILAEAEATGADLIVLGTF
                     VSTVDGRPFLGYTVDHVLAQSTATVVVVAIPEVLPVIGVTERNS"
BASE COUNT         1476 a         3109 c         2903 g         1276 t
ORIGIN      
        1 ggccatgatc accggcgtcg ccgcccgcca gccggtatat cccacgatgc cgcacacgac
       61 gaatatcgta tgcgccgcag gtgccgggcc gcccgcgaca cacgtgccgc ccgccgtgat
      121 ccgcgcggca cggggcgtgc cgccgagtac gccgtcagga gacgtgggtg cgacccgggt
      181 cgcggatcaa cccgatcgtg tgcaccggcc ctgtcaatcg aatgccgggc ctcggccgcc
      241 cgtcatcctt cgcgacgctc aaccgcaccg ggctctggtc ggccggcaga gagaccgtga
      301 cggcgccgac gacggtctcg cccgggttca tgtaacgctc gtgctggggc aaggacgtga
      361 cgtcgagccg gaactcgccc cggtacgagt aacgccgccg caaaaccccc acgcgccggc
      421 ccgtccacac ggtggcgatg cgccacatga cgggcacgcg caacggccgg tgcgacacgt
      481 tcaccatgac gacggggatc acccaccagt cttgcgtgaa ctcgtctttg tcgagcctgg
      541 ccgcctccgc ctcgaagcgg atgccgctga cggagcccgg cagcggccgt gattccatga
      601 gccagagggc ggaacgcacg atcgtgacaa gaagcgcaac ggcgagggca gcgagcgtca
      661 ccgcgcccac aacctccagc accagcgcga acactcccac gaccaacccg tctgcatccg
      721 tcgggtaccc ggcatgccca ccgtgagtgc tcgcggcccg gccgagcccg cacccgcgcc
      781 gcgccctgaa tactcaaagg tcaggcatct gacgctgcat agattacacg gtgccgcgcc
      841 cgccgggcct cagccgccga tctgggacat ggacacctgg tgaaccgcct gcgagccgcc
      901 cacgtacttg cccgggttct cgaagatatc gggagtgtcg cggtcttgcc cggccctctt
      961 cgaggccacc gcggccagga aggcctgggc gatctcctca tcggtgcagc cgccgtcccg
     1021 ccgcatcagg ttgcgcagtg cgtacttgct gtccttacgg gagaaaagac accgcatgac
     1081 gaaaccatcg cccgtgatac gcagcctgtt gcagttcgcg cagaacgggt tggtcatggt
     1141 cgggatcacc ccgatctcgc ccttgccgtc ttgaaaggcc caccggctgg cgggggcgtg
     1201 gtccttctgc gacgaagaga tcttcagcgg ccactgatcg ccgatcatct tcacgatgtc
     1261 ggcggagcgc acgaccttgt ctttcgtcca gttctcctgc ttctcgcccg tctcgccaag
     1321 cggcatgaac tcgatgaagc gcacgctgat gggggtgccg gcctcccacg actcgcgcac
     1381 gtagttggcg aagtcgagga tctcgtcttc gttctggccc cggatgacaa ccgtgttcat
     1441 cttgatgggc gagaagccga cccggtgcgc ctcggcgatg ccggccatga cgacgtcgaa
     1501 gtcgccgcgc cggcggatct tcgcgaactt gtcgggttgc aaggagtcgc agctgatgtt
     1561 cagccggttc aagcccgcct cgcgcagggc ctgggcggca gtgatgccac ccaccttgcg
     1621 ggtaagaagg gagccgttcg tcgtcatcga gatgtcgtcg aaaccgatcg tcgagagttg
     1681 acgcaccacg tcgatcgcgt ccttgcgcat aagcggttcg ccccccgtca ggcgaatggt
     1741 gcggatcccc aggttgtgcg cgatgcgggc cacccggacg agctcaggcg tgtcgagctc
     1801 taaccgagtc agattgaagc gctgatcttc tcgcatgcag tacacgcaac ggaagttgca
     1861 tttgtcggtg agagagattc gcatgtcggt gtgttcccgg ccgaatccgt cggtcagcga
     1921 tgcccgagcg gccttcgtct cgggatccat cgtggtacgc atgatgacca gcctttccgt
     1981 gctgtcgccc cgcggccgtc tcagccacct tcggcgagcg tatgtccgtc gtaggtagcc
     2041 tgtcacgcgc cggcgtcggc acgccggata aatgaggccg aatatgcgcc aggcacgacg
     2101 ttcggcttgt cttcggccgg ccttcaccgg cagcgcatac aatcacggct gtgtccgcgc
     2161 ccccccacgt cagcgatgag gagacccctg cgcgatccgc ggcctcccgc accgtgacgg
     2221 tgatcgtccc gaccaggaac gaggccggca acgtcgggcc cttggtcgct cgcctggcca
     2281 cagcgctatc gggcgtggcc gcagaggtgt tgttcgtcga cgactcagac gacgacacgc
     2341 cccgggccgt tctccacgcc gcccgccggg ggggcctccc ggtgcggctg atccaccgca
     2401 cgcacggcgc gggcgggctc tcgggcgcgg tggtcgccgg gctcaccgca tcccgggcca
     2461 ggtggtgcgt cgtgatggac ggcgaccttc agcacccgcc ggagctgata ccggcgctcc
     2521 tggcgcgcgg cgaggcggaa gaggccgagg tggtggtcgc ctcaaggcac cgcgcgggcg
     2581 gcagttccag cggcctcgac ggctggttgc gtcacgccgt ctcgcgcgcc tcgaccctcg
     2641 tgacccgggg catgttcccg cgcaggctgc acggctgcac cgacccgatg tcaggcttct
     2701 tcgccgtgcg ccgggccagc ctgaacctcg gcgacatgcg gccacgcggc ttcaaggtgc
     2761 ttctcgagat tctcacccac cacaggttcc gcatcgccga ggagcccttc gtgttcgggg
     2821 tgcgctcctc cggctcgtcc aaggcagggg cgggcaacgg cctgcagttt ctcgtgcaac
     2881 tggccgttct gcgcttcggc aggctatcga agttcgggat gatcggcgcc ttcggggccg
     2941 tcatgaacct cgccatcatg gcctggctca tgtgggccgg gaccggctat cttgtcgccg
     3001 cgatcgtcgg cggcgcggtg acgatcgtca tgaatttcgt cttgcaggag tggctggtct
     3061 tcaaagatct gcgccacggt aggcacggct ttgccggtcg tttcgcgcgt tcggtcggtt
     3121 tcgacacggc cgaaaccgcc gtgcgcaccg gcgtcctgtg gctcctcgtg cgctttctcg
     3181 ggtttcacag catcctcgcg caggcgcttc tcttgtgcgt cgggttcctc gcccggttcg
     3241 tctaccgctc cctcgtcgtc tatcggcaag acacggcgga gccgctcacc ttgctgcccg
     3301 acgggttgcc cgtccccgaa gaggcagccc ccggcgactg agcgccgccg cgccgcgttc
     3361 agccggcagc ctgccgcgtg atctctcgcc ctccgactca ccgttccacg gcgggttgcg
     3421 gcgtcgcgta cagggtccag atgccgtcat ggaagagcac ccggccgcgg cgtatctcgg
     3481 cggcggaggc ggcgtcgagg ccggtgccgg cacggtattg gatgacggcg gagcccgaca
     3541 gcaggtagtc gacagccgcc ttctgccaga ccgcgttctt cgcccgtgcc acggcgacgc
     3601 cgtcttcggt caccttgtcg gcgtcgtagg tccatcccgt cacgtcgggc cagagcgggc
     3661 agccggcctt gagatcgcga gaaagcacgt tcatcaccgc gagaatcgtc gggtcgtcgg
     3721 cgaggacaca gccgtcgacg ctcgcggcgg ccgcctgcag gccgcgcgcg ggcggcgccg
     3781 gctgggcacc ccgaagctcg gcaggaagat acacgacaag gattccggcg atcgccacga
     3841 cggtgagcgc cacgcgcccg gcgcgcggtc gcaagagtgc gcagaggcgg ccgaaggcca
     3901 cgccgaggat gagagccaac gcggggttgg tgagcgccgt gtagtgcggg aagaacgagg
     3961 gggagagaag caacacggcc agccccgaga agaacaacgc gacgtacagc cgggaatccc
     4021 tcacggcgag ggccacgact accgcgagga tggccaggac cagcagcaca tagatcagct
     4081 ttctcgtgct gaggccgaaa cccggggtga tcgcgtcgcc gaggatgatg tgcagcttgt
     4141 ggaggatgct ctggtgcacc cgggggcgcc ccatctgatc ggtgacgatt tcacggaaca
     4201 tcgtcgacgg cgcgaacacg aagaacggca ggtagcagac cagagccccg acgacgacgc
     4261 ccacgagaag acgcacccgc cgacgcgcgc ctcgggtgaa gagcacgatg accaccgccg
     4321 gcacgatgtt ccagatgcgg aacgacgtgc tgaaccccag cacgatgccg gcgatgacga
     4381 gccaccgctc gggggcgcgc agccacgacg cccacccggt ggagacgacc ccggcgcggt
     4441 cgacgccggc ggccttcgtg ccgggcggcg cgtcgctgtg cgacacgcgg ccttgcagcg
     4501 cgaggccgac gaggatgcac agcgtgccga acggctcgag aagaaggctg cgctcggtgt
     4561 agatggccat gaaatccaag gcgtagaaga agccgccgaa cgcggccgcc gcatagccga
     4621 agcgtctcag gatcacggcg acgagcccgg cgttgaccgc acccgccgcg atgaagagca
     4681 gccgaccggt gataaggccc acgggatcgc cgacgaggct gccgagccac gcggccgggg
     4741 cagccgcgaa gatgacgccc gggggctgca acagcaggaa gtcccgatac ggcaggcggc
     4801 catgcacgaa cgcgtcggtc gccgcgtagt agacgccgtc gtcgtagtta cgggcctgca
     4861 gcaaccctcc ggtgtgcagc aggtagagga gccgcgcggt gaaggccgtc gccacgatca
     4921 cggcgacgat aagccaccat acccagcgac gtcccttcgg tgcggctggt cggcggccca
     4981 cccgcccgcg cacgcgagcc atcgtcacac gaaccagatc cccccgaatg tctcccacga
     5041 acaaacacat gccaagctgc gcggcgcgcc tcgcccgcca cgcgtcggcg acgctcaccc
     5101 ggccgagcga ggcgccgcca gccagggtat tggcctccgc cgccaccacg ctgggagaac
     5161 cctcaccatc tgccctcgcc ctgcctcgac cgacggcacg aacccctggg ccgaacgttt
     5221 atccttacac tgtggcgctg cgaaccctgg accagtcggc gaagctgaag aacgtgctct
     5281 acgagattcg tggggccgcg ttggccgagg ccgaacggct ggaggccgac gggcagcaca
     5341 tcttgaagct caacacgggc aacccggccg cgttcggttt cgaggcgccg caccagatcg
     5401 tgcgcgacat gatcgccgcc atccccgaag cacacggcta cagccagagc cgcggcatcc
     5461 tctccgcccg gcgcgcggtc gtcgcccgct acgaggagac gcccgggttt ccgcatgtcg
     5521 accccgacga cgtctatctc ggcaacggcg tgtcggagct catcacgatg gtgacccagg
     5581 cgcttctcga cgacggcgac gaagtactca tcccgtcgcc ggactacccg ctctggaccg
     5641 cgatgacgag cctggcgggc ggggtcgccg tgcattaccg ctgcgacccc gacgacgcct
     5701 ggcagcccga cctcgaagac atccgtgcaa agatcaccga ccgcacgaag gcgatcgtcg
     5761 tcatcaaccc gaacaacccg acgggcgccg tctacgggcg ccagagcctg gaggggatcg
     5821 tcgagatcgc ccgtgagcat tccctgctgc tgctggccga cgagatctac gaccgcatcc
     5881 tctacgacga cgcggaacac gtgaacctgg cctccttggc gcccgacctc ttatgcctca
     5941 ccttcaacgg cctgtcgaag acctaccggg tggccggcta ccgcagtggc tggctcgtca
     6001 tcacgggacc tcggcgccac gcggcgggct ttttgcacgg cctaggcctc ctcgcctcga
     6061 cccgcctgtg cccgaacgtg cccgcccagt acgcggtgca ggccgccctc tcgggggtgc
     6121 aatcgatcga cacgctcctc gtccccggcg gtcggctcca cgaccagcgc gacgtggcct
     6181 ggcaggccgt cacgtccatc cccggcgtct cgtgcgcgaa gccgcgcggc gcgctgtacc
     6241 tctttccgcg cctcgacccc gaggtctacg acatccacga cgacgcccgg ttcgtgtacg
     6301 acctgctcgt ctctgaacac gtgttgctcg tgcaaggcac cgggttcaac tggccgacct
     6361 ccgaccggtt ccgcatcgtc accttgcccg aggcgcgggt gctctcaaac gcgatcgagc
     6421 ggatcggcaa cttcctcgcc tcataccggc aatagccgcg ccgcctcggc acacgccgcc
     6481 gaagacccca ccgccccgcc gggcatcagg agttccgttc cgtcacaccg atgacgggca
     6541 gcacctccgg gatggcgacg acgacgaccg tcgcggtgct ctgtgccagc acgtggtcga
     6601 ccgtgtagcc gaggaacggc cggccgtcga ccgtggagac gaacgtgccc agcacgatga
     6661 ggtcggcgcc cgtggcctcc gcctccgcca ggatctcttc gccccgcacc cgcccggcac
     6721 ggacccggcc gcgagccggc acgttctgct cccgggcatg cgacaaggcc tcgcgaacga
     6781 cggagacggc ggcctgcgac gccgcctcgg cctggcgtgc cgagggggct tcggccgaca
     6841 cctcgcggcc cgaatcccgt tccggctgcg tcacgatatg ggccagcacg agatcggccc
     6901 cggtgcgctg ggctatgccg tagccgaatt cctgcgcggc ccgtgagccg gtcgacccgg
     6961 tgacgggcac caagaccttc cgaaacggct ccccggcggc cgggcgcgaa tcgcctctgc
     7021 cgccacgcac gatcaccatc ggcacggggc ttatcgagag caggtcgtcg acgaccggat
     7081 tcaagatccg gccctctcgg aagctgtcac ggctgccgag gccgagcgcg acatagccga
     7141 ggcgcgcctc ggccaacgtc gcatccaacg cggcgccccg tacctgccgc acctccacgt
     7201 cgcggttctc gaagacctgc aggatctcgc gcaggtcggg tcgcttgccc gcgtccgtgc
     7261 cgatcgcgag cagtgtgacg ggcgactccg gcggccaggc gtcatggagc acgcgggcga
     7321 cggccaacga gttcggcccg ccgcgcgtcg gcatcaaaag ccgaccacgc cggatcagca
     7381 ggttgctctc catcgccgcc tcacgttgga gacgtaggcg ctcctcggca ctgccctgcc
     7441 agccgcgcac gatgaggcgc aaaagcggcg gggccatcat cgacgtgacg atcgccatga
     7501 cgacgatcgc cgtgtagacg ctgccgctca aaacgccgat cgtgaggccg acggttgcga
     7561 tgacgacctc cacggcgccg cgcgcgttca ggccgacggc gagggccagg ctctcccggc
     7621 cgccgagccg cgccagccgg gcggcgccga aggccccgcc gaacttcgag ccgacggcca
     7681 ccgccaagat gatgcccgcc cacgcgagcg tcgtcgggtc ggcgaggctc gccaggttga
     7741 cgcgcaggcc cgccgtcgcg aagaacacgg gggcgaagat cgccgcattg accgattgca
     7801 gctgccgtcg ggtgcgttct tgaaagaacc gcgaacggcc gatcaggatg ccggcgatat
     7861 acgccccgag aacggcgtcg ctgcgcgcca gctgggtcgc gaccgcgaag aggaaggtta
     7921 tgagcagggt catgcccatc gcgtctgccc cggtgcggtc tcgtttgcgc acgagtcgca
     7981 agacggcatc cacgccgcgc tgcccgacga agatcgcgat ggcgaggatc acggcgatgg
     8041 ctgcgagcgg atacgcgatc gtgccgaagg tcacgtggct ggtgcgtccg agcgccgcga
     8101 tgatccccag ggcgagccag ccgacgagat cgttgaccat gccgacggcc acggtgacct
     8161 gggcgaagtc gcggcgcatg aggcccagct cgctcaagat cttggcgatg accggcaacg
     8221 aggagatcga gagcgacacg gcgatgaaga gcacgaatgc ggtcgccgtc gaatgctctc
     8281 ccataagcga cgacggcatc cagaacccca ccgcgaggcc cgccgcgaag ggcacggcga
     8341 ggccgccgac cgcaacgaac gtggccggcc ggcccagttt gcgcacgagc gtcaggtcgg
     8401 tctcgaaccc cgtcaagacg agcaagaagg cgacgccgat ccatccgatc gcgttgatcg
     8461 gcgcggccga cgcgagagag tgcggcacga ggaaccgttc cgccgccggc cacaggtgtg
     8521 ccagaaccga cggcccgaga agcaccccgg ccaaaagctc gccgatcaca cgaggctgcc
     8581 cgatccgctg catcagggaa cccagcgcac gtgcgacgac gaggatgacg agaagctgca
     8641 cccacatcag cagaagaccg tgctcggtca gtgaaggcag acccacgggg cctccttccg
     8701 atcgatgcct tgcagccggc ggcggcggcc gcgctgcggc cgcgtgtcgt cccacataac
     8761 ggga
//
LOCUS       DASTNL010000135         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1207239, whole genome shotgun sequence.
ACCESSION   DASTNL010000135 DASTNL010000000
VERSION     DASTNL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207239"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFQ96_04255"
     CDS             <1..822
                     /locus_tag="VFQ96_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HEU0257033.1"
                     /translation="GTEAAVSVIALVGERGREVREFLDDDLGPAGLARSVVVVATSDE
                     PALMRLRAAQAATRIAEWFREGGEHAVLMMDSLTRVAMAQREIGLSAGEPPATRGYPP
                     STFSVLAGLLERAGTGPTGSVTGIYTVLVEGDDHNEPIADAARSILDGHIVLDRALAV
                     AGHFPAIDVLGSISRLASKIVAPERMAAAATVRRMLAERRRAQDLLDVGAYKTGTNRW
                     VDAALRLDDRVDAFLRQDMDDQTGGEAAWRLLAGLAASVGESGAVASPANAESTG"
     gene            899..1339
                     /locus_tag="VFQ96_04260"
     CDS             899..1339
                     /locus_tag="VFQ96_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HEU0257034.1"
                     /translation="MATFHLSGLLRLRTLRESLAAVGLAEARHELSGVRDRRARVGAQ
                     LAGTTVAATGPESLAAIAAARASAQAMIGSLAALEELAGQKAEAAAAAHRLARTEART
                     IRRLSDRFAAEEARKDLHREQVALDEIAITRWGRAAHDGPGQAS"
     gene            1336..2040
                     /locus_tag="VFQ96_04265"
     CDS             1336..2040
                     /locus_tag="VFQ96_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU0257035.1"
                     /translation="MSIAAIVTRMQQIAAGIQRLGADGAYTSGVPGASALATAAAPVG
                     SGPDFAGALAQALTGAAPSSAAPAVHVAAGAPATAVAGAVVDNGTTGADVVADARKYL
                     GVPYVFGGTTTAGLDCSGLVQKVFGDLGISVPRLVTGQMKVGVAVPSLAQAQPGDLIV
                     ADGGDHIAIYAGDGQIIEAPHPGLSVRERSDWITAANTVTIRRIVPSASSASSSASAA
                     PSVTAAALLAGWGASR"
BASE COUNT          269 a          774 c          778 g          287 t
ORIGIN      
        1 ggcacggagg cggccgtgtc ggtcatcgcc ctcgtcggcg aacgcggccg cgaggtgcgg
       61 gagtttctcg acgacgacct cggccccgca gggcttgccc gctccgtcgt cgttgtcgcc
      121 acctccgacg agccggccct catgcggctg cgtgccgcgc aggctgccac ccgcatcgcc
      181 gaatggttcc gcgagggcgg cgaacacgcc gtgctcatga tggattcgct cacccgcgtc
      241 gcgatggccc agcgcgagat cgggctctcc gcgggggagc cgccggccac acggggctat
      301 ccgccctcga cattctcggt gcttgcgggc cttttggagc gcgccggcac gggcccgacg
      361 ggctcggtga ccggcatcta cacggtcttg gtcgagggcg acgaccacaa tgagccgatc
      421 gcggatgcgg cgcgctccat tctcgacgga catatcgtgc tcgatcgcgc cctcgccgtg
      481 gccggccatt tccccgccat cgacgtgctc ggctccatct cgcgcctcgc gagcaagatc
      541 gtcgcaccgg aacgcatggc ggcggcggcg acggtgcggc gcatgctcgc cgaacgccgc
      601 cgggcccaag acctgctcga cgtcggcgcc tacaagacag gcacgaaccg gtgggtcgac
      661 gccgcgctgc gcctcgacga ccgggtcgac gctttcctgc ggcaagacat ggatgaccag
      721 accggcggcg aggcagcctg gcgcctcctc gcggggctcg cggcgtccgt cggcgagtcg
      781 ggtgctgtcg cctcgcccgc gaatgccgag agtaccgggt agcgagggcc gcccgcggcg
      841 tcacgccgtg tgggcgcggg cgatggccgt gtcgaagacc ggaggagagg ggcgcacgat
      901 ggcgacgttt cacctctccg gcctgctgcg gcttcgcacc ctgcgtgaga gcctcgcggc
      961 ggtggggctg gccgaggcac gccacgaatt gagtggtgtg cgagaccggc gtgcccgagt
     1021 cggcgcccag ttggccggaa cgacggtggc ggcgacgggg cctgagagcc tcgcggcgat
     1081 cgccgccgct cgggcgagcg cccaggcgat gatcggcagc ctcgcggccc tcgaagagct
     1141 cgccgggcag aaggcggagg cggcggccgc ggcgcaccgc ctggcgcgca ccgaggcccg
     1201 cacgatccgg cggctgagcg accggttcgc ggccgaagag gcccgaaaag acctgcaccg
     1261 ggagcaggtg gcgctcgacg agatcgccat cactcgctgg ggtcgcgccg cccacgacgg
     1321 ccctggccag gcgtcgtgag catcgccgcc atcgtcacaa ggatgcagca gatcgcggcc
     1381 ggcatccaac gcctgggtgc cgacggcgcg tacacctccg gcgtgccggg cgcctccgcg
     1441 cttgccacgg ccgcggcccc cgtcggctct gggcctgact tcgccggggc tctcgcccag
     1501 gcgctcaccg gtgccgcacc gtcttctgcc gcgcccgctg tccacgtcgc ggcgggtgcg
     1561 cccgccaccg ccgtcgccgg agccgtcgtc gacaacggca cgaccggagc cgacgtggtc
     1621 gccgacgccc gcaagtatct cggcgtgcca tacgtcttcg gcggaacgac gacggccggc
     1681 ctcgactgct ccgggctcgt gcagaaggtc ttcggcgacc ttggcatcag cgttccccgg
     1741 ctcgtcactg gccagatgaa ggtcggcgtg gcggtgccct ccctcgccca ggcgcagccg
     1801 ggcgacctca tcgtcgccga cggtggcgac cacatcgcga tctacgccgg agacggccag
     1861 atcatcgagg ccccgcaccc ggggctgagt gtgcgggagc gcagcgactg gatcactgcg
     1921 gcgaacacgg tcacgattcg ccggatcgtg ccctccgcgt cttcggcctc gtcatcggct
     1981 tccgccgccc cgagcgtcac ggccgccgcg cttctggcgg ggtggggggc gtcgcggtga
     2041 gccgggccgc ggcggtgccg gaggcggcgg ggatggctgc ccgttccgat gcgcggccgg
     2101 ccgcgcgg
//
LOCUS       DASTNL010000136         6920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1207287, whole genome shotgun sequence.
ACCESSION   DASTNL010000136 DASTNL010000000
VERSION     DASTNL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207287"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1797)
                     /locus_tag="VFQ96_04270"
     CDS             complement(85..1797)
                     /locus_tag="VFQ96_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU0257036.1"
                     /translation="MSSRTTTAPDTAEVALAESHHTRNVLLVFAGLMIAMFLASLHQT
                     IFATALPTIVGDLHGVDLMTWVITVYILASTIMLPIYGKIGDLIGRKGIFIGAISIFI
                     IGSIIGGWAGNMTFLILGRAIQGIGGGGLMILAQAIIADVVPARERGKYMGIIGGVFA
                     ISSVAGPLLGGWYTDVIGWRWGLWMNIPLGVLAILAAVFFLHLPKHPKKAVHLDVFGM
                     ALLAIASTGIVLVSTWGGTKYDWDSPQIIGMIVGTVVAGVAFVLVELRVTEPVMPLQL
                     FKKLNFNLMTGAGLAIGVAMFGTLAYIPTYLQMVTGANATQSGLLMIPMMGTVLLTSI
                     VSGALVTKTGRYKWAPISGTLVTALGMLLLSTMTVGMPVWLICVYLATMGFGLGLCMQ
                     IFVLVVQNTFPNAMVGVATATNNYFRQIGASLGSAVVGTLFTTRLIHLLSERMPAAAA
                     GQTGSANDLTPDLVKHLPGPIHDIIVASYNDALAPIFLYILPLIIVATIIVCFVAEVP
                     LKKTIDRGNEHTGGPQGDGSNAASLGTSESAVADEAAGGGHAAAEGAASTAAQEEAAP
                     AGGR"
     gene            complement(1794..2450)
                     /locus_tag="VFQ96_04275"
     CDS             complement(1794..2450)
                     /locus_tag="VFQ96_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0257037.1"
                     /translation="MHGFTEAAETLGLRDRKRQETRGRLERAAVDIVLEEGLDHLTID
                     AISERADVSPRTFFNYFDSKEDAILGVRITDDTERIIARAAEEIHPESLLDGVLQLLL
                     RLLDSSLHDKDLHKRRQQIMREHPELLYRRFSQMNRMLESMTAAVQTLWARLHSSSSP
                     DDLSAGEAQVVLMMCGAAIRSATADVSCHHTAVPAKNFTTVIGRQAATLVREAINKLP
                     "
     gene            complement(2636..3496)
                     /locus_tag="VFQ96_04280"
     CDS             complement(2636..3496)
                     /locus_tag="VFQ96_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0257038.1"
                     /translation="MRIATWNVNSIRTRAARVTDWLVAADVDVLALQEIKCRPEQFPY
                     DLFEEAGYQVEAHGLNQWNGVAFASRLPMADVTTAFDAMPGFEKTPEHPGGHHGLDVP
                     REARALGVSVAGLRLWSLYVPNGRALGDPHYRYKLDWLAALAADTRGWLAAEPNLPLA
                     LMGDWNVAPLDSDVGDPSFVPGESTHISEPERAAFAAFEAAGLTDVVRPIVPSGYTFW
                     DYKQLRFPHDEGMRIDFIMGSAGFASLVTDAGIHRDERKGDKPSDHVPVHVDVSTSDY
                     DEDDDRPMIF"
     gene            3561..4031
                     /locus_tag="VFQ96_04285"
     CDS             3561..4031
                     /locus_tag="VFQ96_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU0257039.1"
                     /translation="MRSSRLVRDAIEFGAVGIGAYVVDVAVFNLLRLFMPGFLGTPVP
                     AKTAGVIAATLVAWLGNRYWTFRRNQRSDVGQEFVEFILVAALGYAVNLGALVVSHYV
                     LGFRSLLADNLAANVVGAALGTVLRFALYRVWVYAPGRGRGGRRHGDGGRRPSR"
     gene            4298..5482
                     /locus_tag="VFQ96_04290"
     CDS             4298..5482
                     /locus_tag="VFQ96_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0257040.1"
                     /translation="MRVLILGGDGFCGWPTSLHLSEAGHEVWIADNLSRRRIDEELGI
                     ASLTPIVSLDERLAAWAEVSGRRITRTDVDVAQDYDGLLALLRDVRPDAIVHFAEQRA
                     APYSMRSSATKRYTVDNNVNATHNLLAAVVEAGLDTHLVHLGSMGVYGYGTAGVQIPE
                     GYLNVSMAGLDGSPVEQEILYPANPGSVYHMTKTLDQLMFAYYVKNDGLRVTDLHQGI
                     VWGTQTDETRRDEHLINRFDYDGDYGTVLNRFLMEAAVGHPLTVHGTGGQTRAFIHIH
                     DTVRCIRLAIENPPEEHGRVRIFNQMTETHRVRDLAKLIAEKTGARIDYGENPRNEAA
                     ENELVVAHDSFTALGLSPLTLEQNLLDEVVEVAHKYAYRCDLEKVPARSRWVRSPVGA
                     DA"
     gene            5487..6752
                     /locus_tag="VFQ96_04295"
     CDS             5487..6752
                     /locus_tag="VFQ96_04295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0257041.1"
                     /translation="MRIAFVTETWQPSVNGVVTRLAAHIDWLTAAGHQVLVIAPRIDG
                     SGAGPQARPGVTVLRIPSFRTWVYGGQPWGWPLLGRVRRMLAAFHPDVVHVVGPFVLG
                     IAGVIAARRLRLPLVASFHTDIAAYAGSYRLGWSRPIIWRILRALHNAAAVNLVTSEH
                     SATLLREHGIRGVRLWRRGVDVARFAAAAARHTAARPPGEPVAGPADEGSLPTALYVG
                     RIADEKHLTALLPLARSGRVRLMLVGDGPDRGRLERLFAGTGTVFTGTLTGPALVEAY
                     AAADAFVFPSVTETLGLVLIEALASGLPIVAVRSPASEELVGRLRTARLVEAARPQDF
                     WPALAELLAVHEPAEEARYAQAQAAGWGWPAAAEQVFACYAGLVARPAPASAVAVPEA
                     QGAGSRQPSSVGRPMQAEQGRSDSVGPAS"
     gene            6763..>6920
                     /locus_tag="VFQ96_04300"
     CDS             6763..>6920
                     /locus_tag="VFQ96_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU0257042.1"
                     /translation="MTSPRQELIDYIADEAVFHGDFTLASGRRAPFYVDLRRVSLDHR
                     VAPLIGRV"
BASE COUNT         1059 a         2304 c         2428 g         1129 t
ORIGIN      
        1 gaaagcccgc tccatcgcgg ccgtgtgaca gaggggcccc gcacccgaag gtgcgaggcc
       61 cctctgagcg aacggagagg gcggtcagcg gccgccggcc ggcgccgcct cctcctgggc
      121 tgccgtgctg gcggcaccct cggcggccgc gtgaccgccg cctgccgctt cgtcggccac
      181 cgcgctctcc gacgtgccga gcgaggctgc gttcgaaccg tcgccctgcg ggccacccgt
      241 gtgttcgttg ccccggtcaa tcgtcttctt caagggcacc tcggcgacga agcacacgat
      301 gatggtcgcc acgatgatga gcggcaggat gtacaggaag atcggggcga gcgcgtcgtt
      361 gtacgaggcg acgatgatgt cgtggatcgg gccgggcaga tgcttcacga ggtcgggggt
      421 caagtcgttc gccgagccgg tctgacccgc ggccgcggcc ggcatccgct cggagagcag
      481 gtggatgagg cgcgtcgtga agagggtgcc gacgactgcg gagccgagcg aggcgccgat
      541 ctggcggaag tagttgttcg tcgccgtcgc cacgccgacc atcgcgttcg ggaacgtgtt
      601 ctgcacgacg agcacgaaga tctgcatgca caggcccagg ccgaagccca tcgtggcgag
      661 gtacacgcag atcagccaga cgggcatccc gaccgtcatg gtcgagagca gaagcatgcc
      721 cagtgccgtg acgagggtgc cggagatcgg ggcccacttg tagcggcccg tcttcgtcac
      781 gagcgctccc gagacgatcg aggtgaggag cacggtgccc atcatcggga tcatgaggag
      841 cccggactgg gtggcgttcg cgccggtgac catctgcagg taggtcggga tgtaggcgag
      901 ggtgccgaac atcgccacgc cgatcgcgag cccggcgccg gtcatcaggt tgaagttcag
      961 cttcttgaag agctgcaggg gcatgacagg ctcggtgacg cgcaactcga cgagcacgaa
     1021 cgccacgccg gcgaccacgg tgcccacgat catgccgatg atctgcgggg agtcccagtc
     1081 gtacttggtg ccgccccagg tgctcaccaa gacgataccc gtcgaggcga tggcgaggag
     1141 agccatgccg aacacgtcga ggtgcaccgc cttcttcggg tgcttgggca ggtgcaggaa
     1201 gaacacggcc gcgaggatgg cgagcacgcc gagcgggatg ttcatccaca agccccagcg
     1261 ccagccgatc acgtcggtgt accagccgcc gaggagaggc ccggccaccg aggagatcgc
     1321 gaagacaccg ccgatgatgc ccatatactt gccgcgctcg cgggcgggca ccacgtcggc
     1381 gatgatcgcc tgggcgagga tcatgagccc gccgccaccg atgccttgaa tggcgcggcc
     1441 gaggatcagg aaggtcatgt tgccggccca cccgccgatg atcgacccga tgatgaagat
     1501 cgagatcgcg ccgatgaaga tgcccttacg gcctatcaag tcgccgatct tgccgtagat
     1561 cggcagcatg atcgtcgagg cgaggatgta gacggtgatg acccaggtca tgaggtccac
     1621 gccgtgcagg tcgccgacga tggtcggcaa ggccgtcgcg aagatggtct ggtggagcga
     1681 ggcgaggaac atcgcgatca tcaggccggc gaagaccagc aagacgttgc gcgtgtgatg
     1741 agattccgcc aaagccactt ctgcggtgtc tggggcggtc gtagtgcggc tgctcatggg
     1801 agtttgttga ttgcctctct gacgagtgtt gcggcctgac ggccgatcac ggtggtgaag
     1861 ttcttggcgg gcaccgcggt gtggtgacaa ctcacgtcgg cggtggccga gcggatcgcg
     1921 gcgccgcaca tcatgagcac gacctgcgcc tcgccggcgc tgaggtcgtc gggtgacgac
     1981 gaggaatgca ggcgagccca cagggtctgc actgcggccg tcatgctctc cagcatgcgg
     2041 ttcatctggc tgaagcgtcg gtacagcagt tcggggtgtt cccgcatgat ctgctggcgt
     2101 cgcttgtgca ggtctttgtc atggagcgag ctgtcgagca ggcgcagcaa tagctgcagc
     2161 acgccgtcga gcaacgactc cggatggatc tcttccgcgg cgcgggcgat gatccgctcg
     2221 gtgtcgtcgg tgatccgcac gcccaggatc gcgtcttctt tgctgtcgaa atagttgaag
     2281 aaggtgcgag gcgagacgtc ggcacgttcg ctgattgcgt cgatcgtcag atggtcgagg
     2341 ccctcttcga ggacgatgtc gaccgccgcg cgttcaaggc ggcctcgggt ctcttgccgt
     2401 ttgcggtctc gcagcccgag cgtctcggcc gcctccgtaa atccgtgcac taatgcatca
     2461 tttcatatct cgcaaacttg cacaagtgca gtgttgcgtt cggcgcactc attcgtcgag
     2521 cgcaatcatg cgcccatgca atcatgcgtt cctgcaaggt ttccgaatgt ggcggagcgc
     2581 cggtcgcggc cgggcgcccg cctcgccgta cggccgagcg gtatcggccg cacggtcaga
     2641 agatcatggg ccggtcgtcg tcttcgtcgt agtccgaggt cgagacgtcg acgtgtaccg
     2701 gaacgtgatc gctcggcttg tcgcccttgc gctcgtcgcg gtggatgccg gcgtcggtca
     2761 cgagcgaggc gaagccggcc gagcccatga tgaagtcgat gcgcatcccc tcgtcgtgcg
     2821 gaaagcgcag ctgcttgtag tcccagaacg tgtagccgga gggcacgatc ggccgcacga
     2881 cgtcggtcag ccccgccgcc tcgaacgcgg cgaaggccgc ccgctccggc tccgagatgt
     2941 gggtcgactc gcccggcacg aacgagggat cgccgacgtc ggagtcgagc ggcgccacgt
     3001 tccagtcgcc catgagcgcc agcggcaggt tcggctcggc cgcgagccag ccccgcgtgt
     3061 cggcggccag cgcggccagc cagtcgagct tgtaacggta atgcgggtca cccagggcgc
     3121 ggccgttggg cacatagagg ctccacaggc gaaggcccgc cacgctcacc cccagcgcgc
     3181 gcgcctcacg aggcacatcc aggccgtggt ggccgcccgg gtgttccggc gtcttctcga
     3241 accccggcat ggcgtcgaac gccgtcgtga catcggccat cggtaggcgg ctggcgaagg
     3301 cgaccccgtt ccactggttc aggccgtgcg cctccacctg gtagccggcc tcctcgaaga
     3361 ggtcataggg aaactgttcc ggccggcact tgatctcttg cagggcgagc acgtccacgt
     3421 cggccgcgac gagccagtcg gtgacgcggg cggcccgggt gcggatcgag ttgacgttcc
     3481 aggtcgcgat gcgcatacct gccacgttac tggcgccccg atcacggcgg gatacgatgg
     3541 cccgtgtgac gcggatgcgt atgcggtcct cgcgtctcgt gcgtgacgcg atcgagttcg
     3601 gcgcggtcgg catcggcgcc tacgtcgtcg acgtcgcggt cttcaacctg ctgcgtcttt
     3661 tcatgccggg tttcctcggc acgcccgtgc ccgccaagac ggccggggtt atcgcggcga
     3721 cgctcgtcgc ctggctcggc aacaggtatt ggacctttcg gcgcaaccag cgctccgatg
     3781 tcggccagga gttcgtggag ttcatcctcg tggcggcgct cggctacgcg gtgaatctcg
     3841 gcgcgctcgt cgtctcgcac tacgtgctcg gcttccgcag cctgttggct gataacctcg
     3901 ccgcgaacgt cgtcggcgcc gctctcggca cggtgttgcg cttcgccctc taccgtgtct
     3961 gggtctatgc ccccggccgc ggccggggcg ggcggcggca cggcgacggg ggccggcgcc
     4021 cgtcgcggta gagcgttcgc gagccgggcc cggtagtgag aggctcgcgt gcggcccgtc
     4081 gggcgcccgg gccgggccgg cagagcgagg ggacgacagg gttcgcgcac gagggtcggc
     4141 cggcctcggt cacggccagc ctgtcagggt gcttatgagg ggcggtgtga gaatcgttgc
     4201 gaggcgtgga atcctggagg cgggcggcgg cggtggagac aggcagctgt acggcgtcgt
     4261 gcggcgagac ggtcacgaag aaggagaggg gatcacgatg cgggtattga tcttgggcgg
     4321 cgacgggttc tgcggttggc cgacgtcgtt acatctgtcg gaggccgggc acgaggtgtg
     4381 gatcgccgac aacctctccc gccgccgcat cgacgaggag ctcggcatcg cctcgctcac
     4441 cccgatcgtc tcgctcgacg agcggctggc ggcctgggcc gaggtgtcgg gccgacgcat
     4501 aacgcgcacc gacgtggatg tggcgcaaga ctacgacggc ctcctcgccc tactgcgtga
     4561 cgtgcgaccc gacgcgatcg ttcattttgc cgagcagcgg gccgcgccgt actcgatgcg
     4621 ttcctcggcg accaagcgtt acacggtcga caacaacgtc aacgcgactc acaacctgct
     4681 cgccgccgtc gtcgaggcgg ggctcgacac gcacctcgtg cacctcggct cgatgggtgt
     4741 gtacgggtat ggcacggcgg gagtgcagat tccggagggc tacctcaacg tcagcatggc
     4801 cggtctcgac ggttccccgg tcgagcagga gattctctac ccggccaacc cgggcagcgt
     4861 ctaccacatg acgaagacgc tcgaccagct gatgtttgcc tactacgtga agaacgatgg
     4921 cctgcgcgtc accgacctgc accagggcat cgtgtggggc acccagaccg acgagacgag
     4981 gcgtgacgaa cacctcatca accgcttcga ctacgacggc gactacggca cggtgctgaa
     5041 ccggttcctc atggaggcgg cggtcgggca tccgctgacc gtgcacggca ccggcgggca
     5101 gacgcgcgcg ttcatccaca tccatgacac ggtgcgctgc atccgtctgg ccatcgagaa
     5161 cccgcccgag gagcacggcc gggtacgcat ctttaaccag atgaccgaga cgcaccgggt
     5221 gcgcgacctt gccaagttga tcgccgagaa gaccggcgcc cggatcgact acggcgagaa
     5281 cccgcgcaac gaggcggccg agaacgagct cgtcgtcgcg cacgacagtt tcaccgccct
     5341 gggactctcg cccctgacgc tggagcagaa cctcctcgac gaggtcgtcg aggtcgccca
     5401 caagtacgcc taccggtgcg acttggagaa ggtccccgcc cggtcgcgtt gggtgcgctc
     5461 gcccgtgggc gctgacgcct gagaccgtgc gcatcgcctt cgtcaccgaa acctggcagc
     5521 cgtccgtcaa cggcgtcgtc acgcggctcg ccgcgcacat cgactggctg acggcggccg
     5581 ggcaccaggt gctcgtcatc gcgccgcgca tcgacgggtc gggagcgggg cctcaggcgc
     5641 ggccgggagt caccgtactg cgcatcccgt cgttccgcac ctgggtctac ggcggtcagc
     5701 cgtgggggtg gccgctcctc gggcgggtgc ggcgcatgct cgcggcgttt caccccgacg
     5761 tcgtgcacgt cgtgggcccc ttcgtcctcg gcatcgccgg ggtcatcgct gcgagacgtc
     5821 tgcgcctgcc gctcgtggcg tctttccaca ccgacatcgc cgcgtacgcc ggctcgtatc
     5881 gtctcggctg gtctcgcccg atcatctggc gcatcctgcg cgcgctgcat aacgccgcag
     5941 ccgtgaacct cgtcacgtcg gagcattccg ccaccctcct gcgggagcac ggcattcgcg
     6001 gcgtgcggct ctggcgccgt ggcgtggacg tcgcgcgatt cgcggccgcc gccgcccgac
     6061 acacggccgc ccggccgccc ggcgaacccg tcgccgggcc ggcagacgag ggctcgctgc
     6121 cgaccgccct ctatgtcggc cgcatcgccg acgagaagca tctaacggcg cttctgccgc
     6181 ttgcccgctc cggccgggtg cgtctcatgc tcgtcggcga cggccccgac cgcgggcgcc
     6241 tggagcggct cttcgccggc accggcacgg tcttcacggg cacgctgacc gggccggcac
     6301 tcgtcgaggc ctacgcggcc gccgacgcct tcgtcttccc ctccgtgaca gagacgctgg
     6361 ggctcgtgct catcgaggcg ctcgcgagcg gcctgccgat cgtcgccgtc aggtcgccgg
     6421 ccagtgaaga gctggtcggc cggctgcgta cggcgcgcct cgtcgaggcc gcccgcccgc
     6481 aagacttctg gcccgccctc gccgagttgc tggcggtgca cgagccggcc gaagaggctc
     6541 ggtatgcgca ggcacaggcg gccggctggg gctggcccgc ggcggcggag caggttttcg
     6601 cctgctatgc gggcctcgtc gcccggcccg cccccgcgtc ggcggtggcc gtgccggagg
     6661 cgcagggcgc aggctcgagg cagccctctt ccgtcggccg gccgatgcag gccgagcagg
     6721 gccgctcaga ttcggtgggc ccggcatcgt agagtggtcg tcgtgacaag cccacgccaa
     6781 gagctcatcg actacatcgc cgacgaggcc gtctttcacg gcgacttcac cttggcgagc
     6841 ggcaggaggg cgccgttcta cgtcgacctg cgtcgcgtca gcctggatca ccgcgtggcg
     6901 ccactcatcg gccgcgtgat
//
LOCUS       DASTNL010000137         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1207498, whole genome shotgun sequence.
ACCESSION   DASTNL010000137 DASTNL010000000
VERSION     DASTNL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207498"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFQ96_04305"
     CDS             complement(<1..336)
                     /locus_tag="VFQ96_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0257043.1"
                     /translation="MTWNPTVPGGAATPRPRVAYAYASGVATLTIDRAEKSNALDEAF
                     FRELNALVSRIHGDDTVRCVVVTGTGRAFSAGADVAELDGLAGHAAYEFAATGQFVFD
                     QLENLPKPVI"
     gene            complement(338..889)
                     /locus_tag="VFQ96_04310"
     CDS             complement(338..889)
                     /locus_tag="VFQ96_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257044.1"
                     /translation="MEEAVVEHAQQTTATTGTASGKDAAARNPSLTPLADALIPAAEG
                     ELSASQAGVPGIFLDQAVTLRPDLAKVLDEAARTLATGMPATELLASWQKTQPDEFQK
                     LFLLVRGAYFLNPEVQRRFHYDGVTPHPLSEAVPPDYTDLLPTVIAAGPRYRRATDNP
                     TAADDKAGDVSAARSAAQTREGR"
     gene            complement(874..>2264)
                     /locus_tag="VFQ96_04315"
     CDS             complement(874..>2264)
                     /locus_tag="VFQ96_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0257045.1"
                     /translation="DYPVDDTESDVSALMWNGVGGSAVVYASQWMRNAPSDFRVKSLD
                     GVADDWPMTYDDLVPFYERVEADFGISGLDGDPAYPDAKGYPMPPVPLGVGGRKVASA
                     MNRLGWHWWPGTNAIATRHYRHLNPSAQRGDCMWGNADYSKCTVDVTHWPDLVASGVE
                     LRTRCRAARIVTGRDGEATGVEYIGPDGGRRVQPARAVILCANGVGTPRLLLLSASES
                     HPDGLANSSGLVGKRLMMHPASAVIGLFDEDLGTERGSWGQSLYSLQFYESDPSRGFL
                     RGAKWGLQPTGGPQRTAGAYPWTSAPIWGEGFQRQLRRRLGRSLLWVSCGEDLPEESN
                     RVELDPRKTDSDGLAAAKLVYKTSENSRRMLDWHNERMAEALRAAGATEVIIAPQIRE
                     SGWHLLGTAVMGTDRTSSVVDPWGETHDVKNLYVFDGSVWPTSSGMNPTATIAALALR
                     WSEHLADGWRRQ"
BASE COUNT          332 a          727 c          828 g          377 t
ORIGIN      
        1 aatgacgggc ttcggcaggt tctccagctg gtcgaagacg aactggccgg tggccgcgaa
       61 ttcgtaggcc gcgtggcctg ccagcccgtc gagttccgcc acatcggcgc cggcgctgaa
      121 cgcccggccc gtgccggtga cgacgacgca gcgcacggtg tcgtcgccgt ggatccggga
      181 gacgagcgca ttgagctcgc ggaagaacgc ctcgtcgagc gcgttgctct tctccgcacg
      241 gtcgatggtg agggtcgcga ctccggatgc gtacgcgtag gcgacccggg ggcgtggcgt
      301 cgccgcgccg ccgggcaccg tgggattcca tgtcatgtca gcgcccctct cgtgtttgag
      361 ccgcactgcg cgcggccgaa acgtcgccgg ccttgtcgtc ggcggctgtc gggttgtcgg
      421 tcgcccgccg gtagcgcggg cccgccgcga tgacggtcgg gagaaggtcc gtgtagtcgg
      481 gcggcaccgc ctcggagagc gggtgtggcg tgacgccgtc gtagtggaac cgccgctgaa
      541 cctcggggtt caagaagtag gcgccgcgca cgagcaggaa gagcttttgg aattcatccg
      601 gctgggtctt ctgccacgac gcgagcagct cggtggccgg catccccgtg gcaagtgtgc
      661 gtgcggcctc gtcgaggacc ttggcgaggt cggggcgcag tgtcacggcc tggtcgagga
      721 agatgcccgg tactcctgcc tgggaagcgg agagttcgcc ttcggccgcg gggatgaggg
      781 cgtcggccag aggggtgagc gagggattcc gggccgccgc atccttgccg gatgccgtgc
      841 ccgtggtcgc cgtcgtttgt tgtgcgtgct ccactactgc ctcctccatc cgtctgcgag
      901 gtgttctgac caacgcagcg ccaaggccgc gatcgtggcg gtcgggttca tgcccgacga
      961 ggtcggccag acgctgccgt cgaagacgta aaggtttttc acgtcgtgcg tctcgcccca
     1021 cgggtcgacg accgaggaag tccggtcggt acccatgacg gcggtgccga gcaggtgcca
     1081 gccgctctcg cgaatctgcg gcgcgatgat gacctcggtg gcaccggccg cccgcagcgc
     1141 ctccgccatg cgctcgttgt gccagtcgag catgcggcgg gagttctcac tggtcttgta
     1201 gacgagcttt gcggcggcca gcccgtcgga gtcggtcttg cgcgggtcga gttcgacacg
     1261 attggactct tcgggcaggt cttcaccaca gctgacccag agcagggaac ggccgagccg
     1321 ccggcgtagt tgccgttgaa agccctcgcc ccagatcggc gcggaggtcc aggggtaggc
     1381 gcctgccgtg cgttgcgggc cgccggtggg ctgcaaaccc catttcgcgc cgcggaggaa
     1441 gccgcgggag ggatcggact cgtagaactg cagcgagtag aggctttgcc cccaggagcc
     1501 gcgctccgtg ccgaggtctt cgtcgaaaag cccgatgacg gcgctggcgg gatgcatcat
     1561 gaggcgcttg ccgacgaggc ccgacgagtt cgcgaggccg tccggatggc tctcggaggc
     1621 tgagaggaga agaagtcgcg gggtgcccac gccgttggcg cagaggatga cggctcgtgc
     1681 aggctgcacg cgccgaccgc cgtcggggcc gatgtattcc acccctgtgg cctcgccgtc
     1741 tcgcccggtc acgatgcgtg ccgcccggca ccgcgtgcgc aactcgacac ccgacgcgac
     1801 gagatctggc cagtgggtga cgtcgacggt gcacttggag tagtcggcgt tgccccacat
     1861 gcagtctccg cgctgcgccg aagggttcaa gtgccgatag tgccgggtgg cgatcgcgtt
     1921 cgtgccgggc caccagtgcc agcccagccg gttcatggct gaggcgacct tgcgcccgcc
     1981 cacgccgagc ggaaccggcg gcatcgggta gcccttcgca tcgggatatg ccggatcgcc
     2041 gtccagaccc gagatgccga agtcggcctc gacccgctcg tagaaaggca cgaggtcgtc
     2101 gtaggtcatc ggccagtcgt cggcgacgcc gtcgaggctc ttcacgcgga agtcagacgg
     2161 ggcgttgcgc atccattgcg acgcgtagac gacggcactg ccgcccacac cgttccacat
     2221 gagggcggag acgtcggatt cggtgtcgtc gaccggataa tcgg
//
LOCUS       DASTNL010000138         7449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_97331, whole genome shotgun sequence.
ACCESSION   DASTNL010000138 DASTNL010000000
VERSION     DASTNL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97331"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="mltG"
                     /locus_tag="VFQ96_04320"
     CDS             <1..1059
                     /gene="mltG"
                     /locus_tag="VFQ96_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0257046.1"
                     /translation="HGRRLLVAAIVVVLLAVGAGLAYSHVRPEIQALSTLFQPDDYTG
                     QGSGSVYVTIRQGDTGSVVATRLHRAGVVKTSHAFYRLLLGTSPAPVFQPGTYELKKR
                     MSSAAALRQLETPGDSIARVTVPEGMSEADVLARLSQATGTPLAILKKTAADPRQFGL
                     PAAAKTLEGYLFPATYTFDPGTSAHEMITRMVDRTREALRADDVPAAREWDTIILASI
                     VQREAGPAGDMGKIARVFQNRLDRGMLLQSDATVAYGTGNTSSVWTTNAERDDAKDPY
                     NTYVHHGLPVGPIGNPGDEAIRAALHPTPGPWLYFVPDNLKTGHTVFSVTGEQHAAAV
                     KRLQEWCKASKENSAYCN"
     gene            1043..1999
                     /locus_tag="VFQ96_04325"
     CDS             1043..1999
                     /locus_tag="VFQ96_04325"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU0257047.1"
                     /translation="MPTATDDASGAAVPAATPFDPAAGARPVVRRLAVLGRPIGHSKS
                     PQLHAAAYRAMGLDWDYSRLEVGPEELPRFVDSLDETWRGLSLTMPLKRTVIDVADEA
                     GPLVTMTGTANTLLLSADGKRRAFNTDVPGFVGALGAAGVTALGTVLILGSGATAACA
                     LCAVARLGAGRVDVATRTPEHAAWLAPLAEGLGVTLAVHPMRLADRTLQVPDLVVSTL
                     PGGVRPDVLITASTRRQALLFDVSYDPWPSPLAAAWQEVHGRVLSGFALLVHQALVQV
                     RVFTAGDPETPLAGEDRVLRAMLDAVGLDEAGWGPGGPTAEG"
     gene            2042..3241
                     /gene="aroC"
                     /locus_tag="VFQ96_04330"
     CDS             2042..3241
                     /gene="aroC"
                     /locus_tag="VFQ96_04330"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU0257048.1"
                     /translation="MLRWLTAGESHGPELVAVLEGLPAGVPLTLDDLHADLARRKLGY
                     GRGARQKFEQDELTFSSGVRHGQSLGSPIALRIGNTEWPKWEKVMSAAPIPADTRLGA
                     RGAALTRPRPGHADLVGMQKYGFDEARPVLERASARETAARVALGAIAKAFLRELGVR
                     TVSHTLQIGPVRVPEDGPLPVPDDVGSLDADPLRCFDSATSLRMVAEVDDAHKAGDTL
                     GGVVEVLAYGLPPGLGSYVHWDRRLDGRLAGALMSIQAIKGVEVGDGFTTTRRRGSAA
                     HDELYASPEGIRRATDRAGGTEGGMSTGDVLRVRAGMKPISTVPHALHTVDVATGEDA
                     TAHHQRSDVCAVPAAGVVAEAMVALVVADAVLEKFGGDSLGQTKRDVAAYLESIPASL
                     RSRIVTP"
     gene            3238..3750
                     /locus_tag="VFQ96_04335"
     CDS             3238..3750
                     /locus_tag="VFQ96_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0257049.1"
                     /translation="MTVVVIGPPAAGKTSVGRELAARLGVRFVDTDDVIVSQHGPIPQ
                     IFASRGEPWFRRVERAAVARSLAAGGVVSLGGGAPLDAETAGLLADHRVILLTVDVPS
                     AAARIGGGGRPLVGDIDSWRDLVERRMPAYRALAKRTIDTSGRAVDELADELAQWVRG
                     AAKAAEEAGA"
     gene            3747..4868
                     /gene="aroB"
                     /locus_tag="VFQ96_04340"
     CDS             3747..4868
                     /gene="aroB"
                     /locus_tag="VFQ96_04340"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU0257050.1"
                     /translation="MSETTTITVGGQKPYPVLVGEGIRDDIAPLLGDEVHKVLIIHQP
                     TLATAAAALQKNLGRHYEVVMAEIPDGEMAKRAEVASFCWQVMGLADFTRSDAIVGFG
                     GGAATDLAGFVASTWLRGVRVVQVPTTLLAMVDAAIGGKTGINTSEGKNLVGTFYPPS
                     AVVADLETLEPLPEVDLLAGFGEVVKYGFIADEAILQTIEKDVAVATSPLSPEFRDIV
                     ERCIKIKADVVGRDLTEQGPREILNYGHTLGHAIEHAERYRWRHGHAVSIGMVYVAEL
                     ARLAGRLSDEVADRHRAILTSLDLPTTYPATRWKSLVATMHRDKKARGDMLRFVVLED
                     IGHPTIMQAPDPSLMYSAYEEIAEKPARRRPTGAAGARH"
     gene            4878..5351
                     /locus_tag="VFQ96_04345"
     CDS             4878..5351
                     /locus_tag="VFQ96_04345"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU0257051.1"
                     /translation="MHSVLVLNGPNLGRLGTRQPEVYGRASLDDVRELLTGAVPGLTP
                     DLRQTDDESTMLHWLYEAADRGLPVIVNAGAWTHYSYALRDAVALVTERAIPVIEVHI
                     SNVHARDGFRAKSVLTPVCTGVIAGFGVDSYLLALQAVGHILSRHIESRSGQAGR"
     gene            5402..5965
                     /gene="efp"
                     /locus_tag="VFQ96_04350"
     CDS             5402..5965
                     /gene="efp"
                     /locus_tag="VFQ96_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU0257052.1"
                     /translation="MASTADIHNGAVLSIDGQLWTVVEFQHVKPGKGGAFVRTKLKNV
                     VTGKTVDRTYNAGAKLEFENVDRHEFQYLYKDGDSFVFMDTADYDQVTVPETVVGDAA
                     NFMLENQQVTIALNNGNPLYVELPASVVLEITYTEPGLQGDRSTGGTKPATLETGYQI
                     QVPLFVDTNTKVKVDTRTGDYLGRISD"
     gene            5990..6409
                     /gene="nusB"
                     /locus_tag="VFQ96_04355"
     CDS             5990..6409
                     /gene="nusB"
                     /locus_tag="VFQ96_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0257053.1"
                     /translation="MSARTKARKRAVDMLYAADVRRRPVQEILAAEAARAASEPARQA
                     SWLYAREIVDGVVDHDDEIDALLRGHAQGWSLERMPAVDRALLRVAVWEMCFGAGVPA
                     PVAIAEAVRAAEELSTEGSPGFVNGLLARIAKETQER"
     gene            6471..7016
                     /gene="pyrR"
                     /locus_tag="VFQ96_04360"
     CDS             6471..7016
                     /gene="pyrR"
                     /locus_tag="VFQ96_04360"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU0257054.1"
                     /translation="MPTRVVLQQADIARALRRISHEIVESNRGTEGLLILGIPTRGVP
                     LAGRIAHIIAQIEPGFDSASRGSLDVTLYRDDLSRSTTRTPSPTRIPQGIDGKTVVLV
                     DDVLYSGRTIRAALDALSDLGRPRAVRLAVLVDRGHRQLPIRADFVGKNLPSAADERI
                     NVHLTEVDGEDSVAIESGATR"
     gene            7016..>7449
                     /locus_tag="VFQ96_04365"
     CDS             7016..>7449
                     /locus_tag="VFQ96_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEU0257055.1"
                     /translation="MRHLLTTVGIGRDDATRILDIAEDMAQIADREVKKLPALRGKTV
                     VNLFFENSTRTRISFEAAAKRLSADVINFTAQGSSVSKGESLKDTAQTLAAIGADAVV
                     IRHPASGAAHTLAMSGWIDAGVVNAGDGTHQHPTQALLDAFT"
BASE COUNT         1160 a         2644 c         2565 g         1080 t
ORIGIN      
        1 cacggcaggc gccttctcgt cgcggcgatc gtcgtcgtgc tgctggccgt cggggcgggc
       61 ctggcatact cccacgtccg cccggagatt caggcgctca gcacgctttt ccaacctgac
      121 gactacaccg ggcagggctc cgggtcggtc tacgtgacga tccggcaggg tgacaccggc
      181 agcgtggtcg ctacgcggct gcacagggcc ggcgtggtga agacctccca cgccttctac
      241 cggctgcttc tcggcaccag cccggcgccg gtgttccagc ccggcaccta cgagctgaag
      301 aagcgcatga gctccgcggc ggccctgcgg cagctggaga cgccgggcga cagcatcgcc
      361 cgggtcacgg tgccggaggg catgtccgag gccgacgtgc tggcccgcct ctcgcaggcg
      421 accggcaccc cgctcgcaat cctcaagaag accgcggcag accctcggca gttcggcctg
      481 ccggcggcgg cgaagacgct tgaggggtac ctgttccccg cgacctacac gttcgacccc
      541 ggcacgagcg cccacgagat gatcaccagg atggtcgatc gcacccggga ggcgctgagg
      601 gccgacgatg ttcccgccgc ccgcgaatgg gacaccatca tcctcgcctc gatcgtgcag
      661 cgcgaggcgg gcccggccgg cgacatgggc aagatcgccc gggtctttca gaaccgcctc
      721 gaccggggga tgttgctgca atccgacgcg accgtcgcct acggcaccgg gaacaccagc
      781 tcggtctgga cgacgaacgc ggaacgagac gatgcgaagg atccgtacaa cacctacgtg
      841 caccacgggt tgccggtcgg cccgatcggc aacccgggcg acgaggcgat ccgcgccgcc
      901 ctgcacccga caccggggcc gtggctgtat ttcgtgcccg ataacctgaa gacgggccac
      961 accgtgttct ccgtgacggg cgaacagcat gctgccgccg tgaagcggct gcaggaatgg
     1021 tgcaaggcca gcaaggagaa cagtgcctac tgcaactgac gacgcctcag gcgcggccgt
     1081 gcccgccgcc acacccttcg acccggcggc cggcgcacgg ccggtcgtgc gccgactggc
     1141 ggtcctcggg cgcccgatag gccattcgaa gtcgccgcaa ctgcacgccg ccgcctatcg
     1201 ggcgatgggg ctcgactggg actattcccg cctggaggtc ggccccgaag agctgccccg
     1261 gttcgtcgac tccttggacg agacgtggcg tgggctctcc ttgaccatgc cgctgaagcg
     1321 caccgtcatc gacgtggccg atgaggccgg cccgctcgtc accatgaccg gcaccgcgaa
     1381 caccctgctc ctctcggccg acggcaagcg ccgggcgttc aacacggacg tgccgggctt
     1441 cgtcggcgcc ctgggcgcgg caggggtcac ggcgctgggt actgtgctga tcctcggttc
     1501 cggcgcgacg gcggcctgcg ccctctgtgc cgtcgcccgg ctgggggccg gccgggtcga
     1561 cgtggcgacg cgcacgccgg agcacgccgc ctggctcgcg ccgctggctg aggggctcgg
     1621 ggtcacactc gccgtccacc cgatgcgtct ggccgaccgc acgctccagg tgcccgacct
     1681 cgtggtgagc accctgccgg gcggcgtgcg gcccgacgtc ctcatcaccg cctcgacgag
     1741 gcgccaggcg ctcctgttcg acgtgtcgta cgacccgtgg ccgagcccgc tcgccgcggc
     1801 ctggcaagaa gtgcacggcc gcgtcctctc gggcttcgcc ctgctcgtgc atcaggccct
     1861 cgtgcaggtg cgggtgttca cggcaggcga cccggagacg ccgctcgcgg gggaggatcg
     1921 ggtgttgcgc gccatgctcg acgccgtcgg cctcgacgag gctggatggg gccccggcgg
     1981 cccgactgcc gaggggtgag gccggccggg gcgccaccgg cccgtgtgcc agaatcgaac
     2041 catgcttcgt tggctcaccg cgggagaatc gcacggcccg gaactcgtcg ccgtcttgga
     2101 gggcctgccg gccggcgtgc ccctgacgct cgacgacctg catgccgacc tcgccaggcg
     2161 caagcttggc tacggtcgcg gcgcgcggca gaagttcgag caggacgagc tcacgttctc
     2221 gagcggtgtg cgacacggcc agagcctcgg cagcccgatc gcgttgcgca tcggcaacac
     2281 ggaatggccc aagtgggaga aggttatgag cgccgcgccg atcccggctg acacgagact
     2341 cggcgcgcgc ggcgctgcgc tgacgaggcc tcgcccgggg catgccgacc tcgtcggcat
     2401 gcagaaatac ggctttgacg aggcacggcc cgtgttggag cgtgcgagcg ccagagagac
     2461 ggccgcccgt gtcgccctcg gcgcgatcgc gaaggctttc ctgcgcgagt tgggcgtccg
     2521 cacggtcagc cacaccctcc agatcgggcc cgtccgggtt ccagaagacg ggccgctgcc
     2581 cgttcccgac gacgtcggct cactcgacgc cgacccgctg cgctgcttcg acagcgccac
     2641 cagcctgcgg atggtggcgg aggtcgacga cgcgcacaag gccggcgaca ccctgggcgg
     2701 cgtcgtggag gtgcttgcct acggcctgcc gccgggcctg gggtcgtacg tgcattggga
     2761 ccggcgcctc gacgggcgtc tcgccggcgc cttgatgagt atccaggcga tcaaaggcgt
     2821 cgaggtcggc gacgggttca cgacgacgcg ccgccgcggc tccgcggccc acgacgagct
     2881 gtacgcctcg ccggagggca tccgccgtgc caccgaccgg gccggaggca ccgagggcgg
     2941 catgtccacc ggagacgtgc tgcgggtgcg ggccggcatg aagccgatct cgacggtgcc
     3001 gcacgcgctg cacaccgtcg atgtggcgac gggggaggac gcgacggccc atcaccaacg
     3061 ctccgacgtc tgcgcggtgc ctgcggccgg cgtggtcgcc gaggcgatgg tcgccctcgt
     3121 tgtggccgac gccgtgctgg agaagttcgg cggtgattcc ctggggcaga ccaagcgcga
     3181 cgtcgccgcc tacctggaga gcattcccgc ctcgctgcgc tcccgcatcg tcacgccgtg
     3241 accgtcgtcg tgatcggtcc gcccgccgcg ggaaagacct cggtcggtcg ggagctcgcg
     3301 gcgcgcctcg gcgttcgctt cgtcgatacc gacgacgtca tcgtctctca gcacgggccg
     3361 ataccgcaga tcttcgcgag ccgcggcgag ccctggttcc gtcgtgtgga acgcgccgcg
     3421 gtcgcccgct cgcttgcggc gggcggggtc gtctccctcg gcggcggcgc gccgctcgac
     3481 gccgagacgg ccggcctgtt ggccgaccac cgggtgattc ttctgacggt cgacgtcccc
     3541 tccgctgccg cccgcatcgg tggcgggggc cggccgctgg tcggtgacat cgactcgtgg
     3601 cgagacctcg tcgagcggcg gatgccggcc taccgcgcgt tggcgaagcg cacgatcgac
     3661 acctcgggcc gggccgtcga cgagctggcg gacgagcttg cacagtgggt acggggcgcc
     3721 gcgaaggcgg ctgaggaggc aggggcatga gcgagaccac gaccataacc gtcggcgggc
     3781 agaagccgta cccggtgctc gtcggcgagg ggatccgcga cgacatcgcg ccgctcctcg
     3841 gcgacgaggt gcacaaggtg ctcatcattc accagccgac gctcgccacg gctgcggcgg
     3901 ccctgcagaa gaacctcggc cggcactacg aggtcgtcat ggccgagatt cccgacggag
     3961 agatggccaa gcgggccgag gtcgcctcgt tctgctggca ggtcatgggg ctcgccgact
     4021 tcactcgcag cgatgcgatc gtcgggttcg gcggcggcgc ggcgaccgac ctcgccggct
     4081 tcgtcgcctc cacctggctg cgcggcgtgc gcgttgttca ggttccgacg accctgctcg
     4141 ccatggtgga tgcggcgatc ggcggcaaga cgggcatcaa cacgagcgag ggcaagaacc
     4201 tcgtcggcac gttctacccc ccctctgcgg tcgtcgccga cctggagacg ctcgaaccgc
     4261 tgcccgaggt ggaccttctg gccggcttcg gcgaggtcgt caagtacggc ttcatcgccg
     4321 acgaggcgat cctccagacg atcgagaaag acgtggccgt ggcgacctcg ccgctcagcc
     4381 cggagtttcg cgacatcgtc gagcgctgca tcaagatcaa ggccgacgtg gtcggccgcg
     4441 acctgacgga gcaggggccc cgcgagattc tcaactacgg acacacgctg gggcacgcca
     4501 tcgagcacgc cgagcgttac cggtggcggc acggccacgc ggtttccatc ggcatggtgt
     4561 acgtcgccga acttgcgcgc ctggccgggc gcctctccga cgaggtggcc gaccggcacc
     4621 gcgccatcct cacctccctc gacctgccga ccacgtatcc cgcgacccgc tggaagtcgc
     4681 tcgtcgccac catgcatcgc gacaagaagg cgcgcggcga catgttgcgc ttcgtcgtgc
     4741 tcgaagacat cggacacccg acgatcatgc aggctcccga cccgtcgctc atgtactcgg
     4801 cctacgagga gatcgcggag aagccggcca ggcgtcgccc gaccggggcc gccggtgccc
     4861 gccactaggc ttgtggcgtg cacagcgtcc tcgtcttgaa cggacccaac ctcggtcgcc
     4921 tcggcacccg gcagccggaa gtgtacggcc gagcgagcct cgacgacgtg cgagaacttc
     4981 tgaccggcgc ggtgccgggt ctcacccccg acctgcgcca gaccgacgac gagtcgacga
     5041 tgctgcattg gctgtacgag gccgccgacc gcgggctgcc ggtcatcgtg aacgccgggg
     5101 catggaccca ctactcttat gccctacgcg atgcggtggc gctcgtgacg gagcgcgcga
     5161 tacccgtcat cgaggtgcac atctcgaacg tgcacgcccg tgacgggttc cgtgcgaaga
     5221 gcgtcttgac tcccgtctgc acgggagtca tcgccgggtt cggcgtcgac tcctacctgc
     5281 tcgcgctcca ggccgttggg cacatcctct cgcggcacat agaatcgagg agcggccagg
     5341 ctgggcggtg actcctccgg caccgccccc caacccccac ggaaacggat cttcctctcg
     5401 catggcatct accgcagaca ttcataacgg cgccgtcctc tccatcgacg gccagctttg
     5461 gaccgtcgtc gagtttcagc acgtcaagcc cggcaagggc ggtgcgttcg tgcggaccaa
     5521 gctgaagaat gtcgtcaccg gcaagaccgt cgaccgcacc tacaacgcgg gggcgaagct
     5581 cgagttcgag aacgtggacc ggcacgagtt ccaatacctg tacaaagacg gcgacagctt
     5641 cgtcttcatg gacacggccg actacgacca ggtcaccgtg cccgagaccg tcgtcggcga
     5701 cgcggcgaac ttcatgctgg agaaccagca ggtgacgatc gcgctgaaca acggcaaccc
     5761 gctctacgtg gagctgccgg ccagtgtcgt gctcgagatc acctatacgg agccgggcct
     5821 gcagggcgac cgttccaccg gtggaaccaa gccggcgacc ctggagaccg gctaccagat
     5881 tcaggtgccg ctcttcgtcg acacgaacac gaaggtcaag gtcgacaccc gtaccgggga
     5941 ctacctcggc cggatcagcg actgaccgaa gaggggcacg ccgccacaca tgagcgcgcg
     6001 aacgaaagcc cgcaagaggg ccgtcgacat gctgtacgcg gccgacgtgc gtcgccggcc
     6061 ggtgcaggag atcctcgccg ccgaggccgc ccgggcggcc tcggagcctg cccggcaggc
     6121 ctcctggctc tacgcccgcg agatcgtcga cggggtcgtg gaccacgacg acgagatcga
     6181 cgcactgctg cgcggccacg cgcaaggttg gagcttggag cgcatgccgg cggtcgaccg
     6241 ggcgctcttg cgggtggccg tctgggagat gtgcttcggc gccggcgtgc cggctcccgt
     6301 cgcgatcgcg gaggccgtgc gggcggcaga ggagttgtcc accgaaggct cgccggggtt
     6361 cgtgaacggg ctgctcgccc ggatcgccaa agagacccag gagcgatgag gtagtgttgc
     6421 ccgaagacat cctttaacat ccgtcccgag aggcgggaaa ggaggtcgat gtgccgacgc
     6481 gcgtcgtcct gcagcaggcg gatatcgccc gtgcgctgcg caggatctct cacgagatcg
     6541 tcgagtcgaa tcgcggcacc gaaggcctcc tcattctggg catcccgacc cgcggcgtgc
     6601 cgcttgccgg tcgtatcgcg cacatcatcg cgcagatcga acccggtttc gacagcgcct
     6661 cgcgcggcag cctcgacgtc acgctctatc gcgacgacct ttcccggtcg acgacccgaa
     6721 cgccgtcgcc gacgcgcata ccgcaaggca tcgacggcaa gaccgtcgtg ctcgtcgacg
     6781 acgtgctcta ctcgggtcgc accatccgtg ccgccctcga cgcgttgagc gacctcggca
     6841 ggccgcgtgc cgtgcgactg gccgtgctcg tcgaccgcgg tcaccgtcag ttgccgatcc
     6901 gggccgactt cgtgggcaag aacctgccga gcgccgccga cgaacgcatc aacgtgcacc
     6961 tcaccgaggt cgacggtgag gattcggtcg ccatcgagag cggggcgacc cgctgatgcg
     7021 gcacctgctc accacggtcg gcatcggccg cgacgacgcg acgcgaatcc tcgacatcgc
     7081 cgaggacatg gcgcagatcg ccgaccgcga ggtcaagaag ctgccggcgt tgcgcggcaa
     7141 gaccgtcgtg aacctgttct tcgagaactc gacccgcacc cggatctcct tcgaggccgc
     7201 cgccaagcgg ctctcggccg acgtcatcaa cttcacggca cagggctcca gcgtctccaa
     7261 gggcgagagc ctgaaggaca ccgcgcagac cctcgccgcg atcggtgccg acgcggtcgt
     7321 catccggcat ccggccagcg gcgccgcgca cacgctggcg atgagcggct ggatcgacgc
     7381 gggggtcgtg aacgcgggcg acggcacgca ccagcacccg acgcaggcgc ttctcgacgc
     7441 gttcacgat
//
LOCUS       DASTNL010000139         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1393766, whole genome shotgun sequence.
ACCESSION   DASTNL010000139 DASTNL010000000
VERSION     DASTNL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393766"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFQ96_04370"
     CDS             <1..371
                     /locus_tag="VFQ96_04370"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU0257056.1"
                     /translation="PIADTTLERTSFFITIEFMLSFVELLIDSLPGVLLRINSHLLDN
                     APPLVGTFPSWFPNPLRDQQLAGDMLWFIAQFTDLPIIIVLFLRWMRTDRGEAEKVDE
                     LSDEEMEALMQEHLHGRREP"
     gene            complement(386..2005)
                     /gene="lysS"
                     /locus_tag="VFQ96_04375"
     CDS             complement(386..2005)
                     /gene="lysS"
                     /locus_tag="VFQ96_04375"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU0257057.1"
                     /translation="MIDENMSRTASARRAGGHGGTATVGGPAPRPSRAARPAGGEDEE
                     AQSEAREQKAVRLAKRQRLIDQAAGPEAGAYPVTVPVTDTILAVRQRFDGTLEPDARS
                     GQSVGVAGRVVHARNTGKLCFASLQAGDGVRIQAMVSLAEVGADSLAAWKEYVDLGDH
                     VFVSGEVVASRRGELSIMVRDWRIAAKAIRPLPNLHNELNEETRVRRRYLDLIARPAA
                     RDMVLRRAAVVASLRRTFAERGFVEVETPMLQTMHGGAAARPFVTHSNAFDTDLYLRI
                     APELYLKRAVVGGIERVFEINRNFRNEGADSTHSPEFAMLELYQAYGDYNAMADLTRV
                     MVQNAAEAVTGGGQRVTWADGTEYDLGGTWRSIGMYESLSEAAGREITPQTPEDDLRR
                     LAREVSVEVAHPNRGKYVEELWEHFVRPGLDEPTFVRDFPVETSPLVRAHRSTPGVVE
                     KWDLYVRGFELATGYSELVDPVVQRERFVDQARQAAAGDDEAMRLDEEFLTALEYGMP
                     PTGGMGMGIDRLLMALTGLGIRETVLFPLVK"
     gene            complement(2088..>2769)
                     /locus_tag="VFQ96_04380"
     CDS             complement(2088..>2769)
                     /locus_tag="VFQ96_04380"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU0257058.1"
                     /translation="CTAAGVDLVFAPTPDVVYPEGDPGVRVSAGPLGDVLEGQSRPGH
                     FDGMLTVVVKLLGIVHGDTVVFGQKDAQQVFLVRQAVRDLNLPVDVVAVPIVREPDGL
                     ARSSRNRYLSAAERGDARVLSRALTAMAREAAEGGARPKAEGDAAGPGGSAAAVRRAG
                     LDVFAGTPAAKLDYLAVVDPATFTEVAADFRGEALAIVAAKVGTTRLIDNKTIVMGQA
                     RRLDRPLG"
BASE COUNT          406 a          993 c          951 g          419 t
ORIGIN      
        1 tgccgatcgc cgacaccacg ctggagcgca cgagcttctt catcacgatc gagttcatgc
       61 tcagcttcgt cgagctgctc atcgactcgc tgcccggagt gcttctgcgc atcaactcgc
      121 atctgctcga caacgcgccg ccgctggtcg gcaccttccc ctcctggttc ccgaatccgc
      181 tgcgcgacca acagctggcc ggcgacatgc tctggttcat cgcgcagttc accgacctgc
      241 cgatcatcat cgtgcttttc ctgcgatgga tgcgcaccga ccgcggcgag gcggagaagg
      301 tggacgagct gagcgacgaa gagatggagg cgctcatgca ggaacacctg cacgggcgcc
      361 gcgagccgta acaggtcggg cgagcctact tgacgagcgg gaacaacacg gtctcgcgaa
      421 tgccgaggcc ggtgagcgcc atcaacagcc ggtcgatgcc catccccatg ccacccgtgg
      481 gcggcatgcc gtattcgagg gccgtcagga actcctcgtc gagccgcatc gcctcgtcgt
      541 cgcccgccgc ggcctgccgc gcctgatcga cgaaccgttc gcgctgcacg accgggtcga
      601 cgagttccga atacccggtt gccagctcga agccgcgcac gtagagatcc catttctcga
      661 cgacgccggg ggtgctgcgg tgcgcgcgca cgagcgggct cgtctcgacg ggaaagtcac
      721 gcacgaacgt cggctcgtcg aggcccgggc gcacgaaatg ctcccacagc tcctccacgt
      781 acttgccgcg gttcgggtgt gcgacctcga cgctgacctc acgggcgagg cgtcgcaagt
      841 cgtcctcggg ggtctgcggg gtgatctcgc gcccggccgc ctccgacagg ctctcgtaca
      901 tgccgatcga acgccaggtg ccgccgaggt cgtattcggt gccgtcggcc cacgtgactc
      961 tctgcccgcc gccggtcacc gcctccgccg cgttctgcac catcacgcgg gtgagatcgg
     1021 ccatcgcgtt gtagtcgccg taggcctggt agagctcaag catcgcgaac tcgggtgaat
     1081 gggtcgagtc agcgccctcg ttgcgaaagt tgcggttgat ctcgaaaacc cgctcgatgc
     1141 cgcccacgac ggcgcgtttc aggtatagct cgggggcgat acgcaggtag aggtcggtgt
     1201 cgaacgcgtt ggaatgggtg acgaaggggc gcgccgccgc tccgccgtgc atggtctgca
     1261 gcatcggcgt ctccacctcg acgaagccgc gttcggcgaa ggtgcgacgc aggctggcga
     1321 cgacggccgc ccgccgaagc accatgtccc gggcagccgg gcgggcgatg aggtcgaggt
     1381 aacggcggcg aacccgcgtc tcttcgttga gctcgttgtg caggttcggc agcggccgaa
     1441 tcgccttcgc ggcgatacgc cagtcgcgca ccatgatcga gagctcgccc cggcggctgg
     1501 cgacgacctc gccggagacg aagacgtgat cgcccaggtc gacgtattcc ttccaggccg
     1561 ccaaggagtc ggcgccgacc tcggcgaggg agaccatcgc ctgaatgcgc acgccgtcgc
     1621 cggcctgcag gctggcgaag cagagctttc ccgtgttgcg ggcatgcacg acccgcccgg
     1681 cgacgcccac gctctgcccc gaccgcgcgt cgggctccag ggtgccgtcg aagcgttgac
     1741 gcaccgcgag gatcgtgtcg gtgacgggca ccgtgacggg atacgcgccg gcctcggggc
     1801 ccgccgcctg atcgatgagc cgctgccttt tggccaggcg caccgccttc tgctcccggg
     1861 cctcgctctg agcctcctca tcctcgccgc cggccggccg ggcggcgcgc gacgggcgag
     1921 gcgccggccc gcccaccgtg gcggtgccgc cgtgcccgcc cgcgcggcgt gccgaggcgg
     1981 tgcgactcat gttctcgtcg atcatggctg tgaaacactt ccgctcagtt ctcggtgacc
     2041 cctcgtggcc cggcgacgcc ggcggccggg gcccggtcga agccctctca gccaagcggc
     2101 cgatcgaggc gccgggcctg gcccatgacg atcgtcttgt tgtcgatcag cctcgtcgtg
     2161 ccgaccttcg ctgccacgat cgcgagcgcc tcgccccgaa agtctgccgc gacctcggtg
     2221 aaggtcgcgg ggtcgacgac ggccaggtag tccagcttag cggcgggtgt gccggcgaag
     2281 acatcgagac ccgcccgacg cacggcggcc gcgctgccgc cggggccggc ggcgtctccc
     2341 tcggccttcg gccgggcccc gccctccgcc gcctcccggg ccatagccgt gagcgcccgg
     2401 gagaggacac gggcgtcgcc ccgctcggcg gcactcagat agcggttgcg gctggagcgg
     2461 gccaggccgt cgggctcccg cacgatgggc accgccacca cgtcgaccgg caggttcagg
     2521 tcgcgcaccg cctgacgcac gagaaagacc tgctgggcgt ccttctggcc gaagaccacc
     2581 gtgtcgccgt gcacgatgcc gagaagcttg acgacgacgg tgagcatccc gtcgaagtgc
     2641 cccggccggg actggccctc gagcacgtcg ccgagagggc cggccgagac gcgcaccccg
     2701 gggtcgccct cggggtagac gacgtcgggc gtcggcgcga agacgaggtc gacgcccgcc
     2761 gcggtgcag
//
LOCUS       DASTNL010000140         6694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_653388, whole genome shotgun sequence.
ACCESSION   DASTNL010000140 DASTNL010000000
VERSION     DASTNL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653388"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VFQ96_04385"
     CDS             <1..1226
                     /locus_tag="VFQ96_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0257059.1"
                     /translation="GVPQVMMAVATQGSRIRSRVGFVKALASALCIGTGGSVGREGPI
                     VQIGSSLASSVGQRMRLPEGRLRLIVACGAAGGIAATFNAPLMGAFFGFELILRLFSI
                     DAFCALLVSALAADVIDQQFFGQAPFFGGFPQLGVQHLVEYALVAVLGVLSGLIGIGF
                     KTVLYKVQDLCDLPFRGRLAWARPAIGGVALGALLFFLPQMYGVGYTVVGEVFSDPGR
                     FALWLVVAMMAGKVLGASITLGNGGSGGVFGPSVFTGAMAGVAFGLLVRALFGPIAGE
                     PVLYGMVAMGAVVASAAQAPLTAAASVVEMTGDFALTLPAILAAAIAAAVSKSLSYGT
                     IYTTKLLRRGIDVEEDLPGPVVVRARRRDATPGTASRPPRFRNARPGTSAPGGETAGR
                     KPQGHKPKGHRAKGR"
     gene            complement(1262..1642)
                     /locus_tag="VFQ96_04390"
     CDS             complement(1262..1642)
                     /locus_tag="VFQ96_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEU0257060.1"
                     /translation="MAKTAIRTARAPQPAGPYSQAIEAGGVIYTAGFGPQDPQTGEVA
                     PTVAEQTRQALRNIGAVLEERGLTLKDCVRTTVHLADLADFAEFNAAYGEFFSEPYPV
                     RTTVGSELSGIRVEINAIAVRPER"
     gene            1891..3261
                     /locus_tag="VFQ96_04395"
     CDS             1891..3261
                     /locus_tag="VFQ96_04395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0257061.1"
                     /translation="MTTQHADHRAAGLRAALAGRAILPDDPAYETARLAWNRAADQRP
                     LAVATPESIAEVADVVRAAARHGLRVAPQSTGHAAGALAGTDLSDVILLSLARLRGVR
                     IDPDSCTATVSGGTLWREVLEAAAPYGLTALHGSAGDISVAGYLLHGGLSFYGRQHGL
                     AVGAVRSAQLVTAQGDIVTASHEENPELFWALRGGAGGFGVVVELEIDLLPYADVFAG
                     MLLWDASKAADVIPAWAAWTREAPESVTTSLRVMHFPPLEELPAFLRSRSVVVIDGAV
                     LEQDDRAAALVRPLRGLGPEIDTFERIPSADLVHMHMDPPEPTPAASAHAMIGGLPAE
                     ATGAFLAAAMEARPMISEIRHVGAAFSRQPARPGAVSSLDGDYAMSAIQVIPAPHLAP
                     AATQATHAVVDALASWHAPSLAATFIDEPGAEPERIFGTCLTRLSDLKRRHDPNDMFA
                     TAHPLA"
     gene            complement(3457..3538)
                     /locus_tag="VFQ96_04400"
     tRNA            complement(3457..3538)
                     /locus_tag="VFQ96_04400"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3502..3504),aa:Tyr,seq:gta)
     gene            3665..4165
                     /locus_tag="VFQ96_04405"
     CDS             3665..4165
                     /locus_tag="VFQ96_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU0257062.1"
                     /translation="MADSSFDVVSKVDKMEADNAVNQATKEVAQRYDFKNVGASVAWS
                     GEKILLKANSEERVRAILDVVQSKFVKRGISLKALDAGEPFASGKEYRIEAALKQGIP
                     QDAAKKLGKVLRDEAPKSVKSQIQGDELRVTSKSRDDLQTAIALLKKADVDVALQFVN
                     FRQGMA"
     gene            complement(4311..5705)
                     /locus_tag="VFQ96_04410"
     CDS             complement(4311..5705)
                     /locus_tag="VFQ96_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0257063.1"
                     /translation="MTKLRLAIVGAGPSGIYAADILLKTERDFDVSIDLFDRLPAPYG
                     LVRYGVAPDHPRIKGVVNSLRAILDRGDIRVYGNVDYGTDITLDDLRRHYNAVIFATG
                     AMHTADLAIPGIGLAGSFGAAEFVSWYDGHPDVPRDWPLEAREVAVIGNGNVALDVSR
                     ILAKHAEDLLPTDVPDNVYRRLESSPVTDVHIFGRRGPMQVKFTPLELRELGELRDVQ
                     MVLADEDFDYDEASKAAIAGNKQVMVIDRILRSWREREPAAAGRRIHLHFYARPVELV
                     DDGAGRVGALRYERTAPDGKGGVQGTGEMREVTVQGVYRAVGFFGAPLPGIPFDRAHG
                     VIPNREGQVLHFGDGAAEEDGREPMPGVFATGWVKRGPRGLIGRTKSDAMETIATLLA
                     AEGDWWAPSAPDEAAVRRLLDERGIRYTGLDGWHNLDAHEIALGAARDRARIKVVPRD
                     EMVRVSRGEDSPRS"
     gene            complement(5769..6692)
                     /locus_tag="VFQ96_04415"
     CDS             complement(5769..6692)
                     /locus_tag="VFQ96_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0257064.1"
                     /translation="MRFTDSLADVTSRYLLEAGGKRVRPMLTLLCAQLGDGASPGVVT
                     AAIAVEITHLASLYHDDVMDEAQLRRGVPATHTVWGNSVAVLTGDLLFARASKLIASL
                     GERAIQLQADTFERLCLGQLHETTGPQGDEDEIEHYLGVLSDKTGSLIAAAAEFGVVF
                     SGAQQAFERPLRTFGENVGVAFQLVDDVIDLSGERDDTGKPAGTDLRAGVATLPLLRL
                     RRRAATDGAARDLMQALDEHVIGVPEGAGDDEAARQAIAELREHPVTAQTRDEARDWA
                     RRAVESLAPLPDGLVKRSLSRFAVSIVERSK"
BASE COUNT         1012 a         2308 c         2341 g         1033 t
ORIGIN      
        1 acggcgtgcc ccaggtgatg atggccgtcg ccacgcaggg cagccgcatc cgttcgcggg
       61 tcgggttcgt caaggcgctc gcctccgcac tgtgcatcgg cacgggcggg tcggtcggcc
      121 gcgaggggcc gatcgtgcag atcggatcgt cgctcgcctc gagtgtcggg caacgcatgc
      181 gcctgccgga ggggaggctg cggctcatcg tcgcctgcgg tgcggccggc ggcatcgcag
      241 caacgttcaa cgccccgctg atgggggcct ttttcgggtt cgagctgatc ctgcgcctct
      301 tctccatcga cgcgttctgc gctctgctcg tctcggcgct ggcggcagac gtgatcgacc
      361 agcagttctt cggccaggcg ccgttcttcg gcggctttcc gcaactgggc gtgcagcacc
      421 tcgtcgagta cgcgctcgtc gccgtgctgg gcgtgctttc cggcctcatc ggcatcggct
      481 tcaagaccgt gctctacaag gtgcaagatc tctgcgacct gccgttccgc ggccgcttgg
      541 cctgggcgcg cccggcgatc ggcggagtcg cgctcggggc cctgctgttc ttcttgccgc
      601 agatgtacgg cgtcggatac acggtggtcg gcgaggtctt cagcgatccc ggccgcttcg
      661 cgctctggct ggtcgtcgcg atgatggccg gcaaggtgct gggggcgagc atcacactcg
      721 gcaacggcgg ctccggcggc gtgttcgggc cctccgtctt cacgggcgcg atggcgggtg
      781 tcgccttcgg gcttctggtg cgcgcgctct tcggcccgat cgccggcgag ccggtgctgt
      841 atgggatggt ggcgatgggc gcggtcgtgg cctcggccgc gcaggcgccc ctcacggcgg
      901 cggcgagcgt cgtcgagatg acgggtgact tcgccttgac gctgccggcg attctcgcgg
      961 cggcgatcgc cgccgccgtg tcgaagagcc tcagctacgg cacgatctac acgacgaagc
     1021 ttctgcgccg aggcatcgac gtggaggagg acctgccggg ccccgtcgtg gtgcgggcac
     1081 gccggcgaga cgcgacgcca ggcaccgcct cccgaccgcc acggttccgg aatgcccggc
     1141 ccggcacctc ggcgccgggc ggcgagactg cagggcgcaa gccgcaaggc cacaagccga
     1201 aggggcaccg agcgaagggg cgctgacgcc tcgaacgggg cgcgtcacca cccgggtcac
     1261 gtcaccgttc gggccgcacc gcgatcgcgt tgatctcgac gcggatgccc gaaagctcgg
     1321 agccgacggt ggtgcgcacc gggtatggct ccgagaagaa ctccccgtac gcggcgttga
     1381 attccgcgaa gtcggccaga tcggccaggt gcacggtggt gcgcacacag tctttcaggg
     1441 tgaggccgcg ttcctctaat accgcgccga tattgcgtag cgcctgcctg gtctgctcgg
     1501 cgaccgtcgg cgcgacctca ccggtctgcg gatcctgcgg cccgaacccg gccgtgtaga
     1561 tcacgccgcc cgcctcgatc gcctggctgt aggggccggc gggttgcggg gcccgcgcgg
     1621 tgcggatggc ggtcttcgcc atgctcttct ccttcgttgg gtttcgaatg attcccacgc
     1681 cctgccggtg ggcaccaggc cgcacgggaa aacgacacag aactgggaaa gatttgacgc
     1741 accgctgagg tagcacggtg tccaggcaga gcatactggg ctcactcgcc acggcgcagc
     1801 ggtgactaag acagcgtcac ccgcatgatc gcacggagaa tttcctcgcg acgcccgaag
     1861 ccgaagcccg agaagcggaa gaaggacgcc atgaccacgc agcacgccga ccaccgtgcg
     1921 gcaggccttc gggccgccct cgcaggccgc gccatcttgc cggacgaccc ggcctacgag
     1981 acggcacggc tcgcgtggaa tcgtgccgcc gaccagcgac ccctcgcggt ggcgacgccg
     2041 gagagcatcg cagaggtcgc agacgtcgtg cgcgccgcgg cgcggcacgg cctccgcgtc
     2101 gcgccgcaat ccacgggcca cgcggccggg gcgctggcgg gtaccgacct ctccgacgtg
     2161 atcctccttt cgctggcgcg gctgcgcggg gtgaggatcg acccggattc ctgcaccgcg
     2221 accgtctccg gcggcacgct gtggcgcgag gttctcgagg ccgccgcgcc ctatgggctg
     2281 acggccttgc acggtagcgc gggcgacatc tcggtcgcag gctacctgct gcacggcggg
     2341 ctgtcgttct atggccggca acacggcctg gccgtgggcg ccgtgcgttc ggcgcagctt
     2401 gtcaccgcgc agggcgacat cgtcacggca agccacgagg agaaccccga gctgttctgg
     2461 gctcttcgag gcggcgcggg cgggttcggc gtcgtcgtgg agctcgagat cgatctcttg
     2521 ccctacgccg acgtgtttgc cgggatgctt ctgtgggacg cgtcgaaggc cgccgacgtg
     2581 atcccggcct gggcggcgtg gaccagagaa gcaccggaga gcgtgacgac gtcgctgcgc
     2641 gtcatgcact ttccgccgct cgaggagctg ccggcgtttc tgcgcagtcg ctccgtcgtc
     2701 gtgatcgacg gcgctgtcct ggaacaggat gaccgggccg ccgccctcgt gcgcccgttg
     2761 cgagggctcg gccccgagat cgatacgttc gagcgcatcc cctcggccga tctggtgcat
     2821 atgcacatgg acccgccaga gcccacgccg gcggcgtcgg ctcacgccat gatcggcggg
     2881 ctgccggccg aagcgacggg ggcgttcctc gcggcggcga tggaggctcg gccgatgatc
     2941 tccgagatcc ggcacgtcgg cgcggcgttc tcccgccagc cggcgcggcc aggagccgtc
     3001 tcaagcctcg acggcgacta cgcgatgagc gcgatccagg tcatccccgc gccgcacttg
     3061 gccccggcgg cgacgcaggc gacgcacgcc gtggtggatg cgctggcatc gtggcacgcg
     3121 ccgagccttg ccgcgacgtt catcgacgag ccgggggcgg agccggagcg tatcttcggc
     3181 acgtgcctca cccggctttc cgacctgaaa cgtcggcacg acccgaacga catgttcgcc
     3241 accgcgcatc cgctcgcctg agagaccggg ccgccggctg ccgacgcaca ccgcatgccc
     3301 cgcacgcacg aggccataca cagtcagctc caaccagtcc gaggcgagct tcggcacaca
     3361 acgcacacac acgaggttgt acgggccgat ggacaccgaa aagttgagag gcccgggcac
     3421 gcgacttccg cgtgattccg ggcctcttcg cgacggtggc gagtggagga ttcgaacctc
     3481 cgtaggctac gccagctgat ttacagtcag atccctttgg ccgcttgggt aactcgccat
     3541 acgcccccgg ccacgtgtat tcacctgacc ggatgcgcct gtcaatacta tgcgatctgc
     3601 cccgcccgag aaaacctcgc gggcacgcgg aggggcgcgg ggcttcttgc ctacactggc
     3661 tgacatggca gattcctcgt tcgacgtggt cagcaaggtc gacaagatgg aggcggacaa
     3721 cgccgtcaac caggcgacca aagaggtggc ccagcgctac gacttcaaaa acgtcggcgc
     3781 gtcggtcgcg tggagcggcg agaagatcct gctgaaggcg aacagcgagg agcgggtgcg
     3841 ggcgatcctc gacgtcgtgc agtccaagtt cgtgaaacgg ggcatctcgc tgaaggctct
     3901 ggatgccggc gagccgttcg ccagcggcaa ggaataccgc atcgaggcgg cgctgaagca
     3961 gggcatcccg caagacgcgg cgaagaagct cggaaaggtt ctgcgcgacg aggcccccaa
     4021 gagcgtgaaa agccagattc aaggcgacga gctgcgcgtg acgtcgaaaa gccgcgacga
     4081 cctgcagacc gcgatcgccc tcttgaagaa ggccgacgtc gacgtcgcgc tccaattcgt
     4141 gaatttccgt caaggcatgg cctgactact tccgcccaga cggcctgtgt ccgcggtcga
     4201 gacgggtcgg cgcggtgtgg atcgcgcggc cgacgaagac gtagccgaac cgcagcagga
     4261 acgcgaccgg gaaggagccg ctagccggta agcctgtgcc acggcccgcg ctatgaccgg
     4321 ggagaatcct caccgcggga gacgcgcacc atctcgtcgc gcggcacgac cttgatgcgt
     4381 gcccggtcgc gggcggcgcc gagggcgatc tcgtgggcgt cgaggttgtg ccagccgtcg
     4441 aggccggtgt accggatgcc tcgctcgtcg agaaggcgac gcaccgcggc ctcgtcgggc
     4501 gccgacggcg cccaccagtc gccctcggcg gcgagaaggg tggcgatggt ctccatcgcg
     4561 tccgacttgg tgcggccgat gaggccacgc gggccccgtt tgacccagcc cgtcgcgaag
     4621 acgccgggca tcggctcccg gccgtcctcc tccgcggccc cgtcgccgaa gtgcagcacc
     4681 tgaccctccc tgttggggat gacgccgtgc gcccggtcga acgggatgcc gggaagcggg
     4741 gcaccgaaga atccgaccgc ccgatagacg ccctgcaccg tgacctcgcg catctctccg
     4801 gtgccctgca cgccgccctt gccgtcgggc gcggtgcgct cgtaccgaag agcaccgacc
     4861 cgccccgcac cgtcgtcgac cagttcgacg gggcgtgcgt agaagtgcag gtggatgcgc
     4921 cggcccgccg cagccggctc ccgttcacgc caggagcgca agatgcggtc gatgaccatg
     4981 acctgcttgt tgccggcgat ggcggccttc gacgcctcgt cgtagtcgaa gtcttcgtca
     5041 gccaagacca tctgcacatc gcgcagctcg ccgagctccc gaagctccag cggggtgaac
     5101 ttcacctgca tgggcccccg cctgccgaag atgtgcacgt cggtgacggg tgacgactcc
     5161 agccggcggt acacgttgtc gggaacgtcg gtgggcagca ggtcttcggc atgcttggcg
     5221 aggatgcgag agacgtcgag ggccacgttg ccgttgccga tcaccgcgac ctcgcgcgcc
     5281 tcgagcggcc agtcgcgggg cacgtcgggg tggccgtcgt accagctgac gaactcggcg
     5341 gcgccgaacg agccggcgag gccgatgccg gggatcgcga ggtcggcggt gtgcatcgct
     5401 cccgtcgcga agatgacggc gttgtagtgc cgccgtaggt cgtcgagcgt tatgtcggtg
     5461 ccgtagtcga cgttgccgta gacgcgaata tcaccccggt cgaggatggc ccgcaacgag
     5521 ttcacgacgc ccttgatgcg cgggtggtcg ggggcgaccc cgtagcgcac gagcccgtac
     5581 ggggcgggca gccggtcgaa aaggtcgata gagacgtcga agtcacgttc cgtcttcagc
     5641 aggatgtcgg ccgcgtaaat acccgacggc ccggccccga cgatcgccag cctcagcttg
     5701 gtcatgccgt gttcatcctc tcgcccgccg ccgcggccgc ccttcaggca ggcggcccgg
     5761 cgcggggctc acttcgagcg ttcgacgatc gacaccgcga accgcgacag cgatctcttc
     5821 acgagcccgt cgggcagcgg tgcaagggac tcgaccgcgc gcctggccca atcgcgggcc
     5881 tcgtcgcggg tctgggcggt gacgggatgc tcgcgcagtt ccgcgatcgc ctgccgggcg
     5941 gcctcgtcgt ctccggcacc ctccggcacc ccgatgacgt gctcgtcgag cgcctgcatc
     6001 aggtcgcgcg ccgccccatc cgtggctgcc cgccggcgca gacgcaagag cggcagcgtc
     6061 gcgacgccgg cccgcaggtc ggttcccgcg ggcttgcccg tgtcgtcgcg ctcaccggag
     6121 aggtcgatga catcgtcgac cagttgaaag gcgacgccga cgttctcacc gaaggtgcgc
     6181 agcggccgct cgaacgcctg ctgcgcgccg gagaagacca ccccgaactc cgccgccgcc
     6241 gcgatcaacg accccgtctt atcagagagc accccgaggt agtgctcgat ctcgtcttcg
     6301 tcgccctgcg ggcctgtcgt ctcgtgcagc tggccgaggc agagccgttc gaacgtgtcg
     6361 gcctgcagct ggatggcccg ctccccgagc gacgcgatca gcttgctggc cctcgcgaac
     6421 agcagatctc ccgtgagcac cgcgacggag ttgccccaca ccgtgtgcgt ggcgggcaca
     6481 ccgcgacgca gctgcgcctc gtccatcacg tcgtcgtggt aaagcgatgc cagatgtgtg
     6541 atctcgacgg cgatcgcggc ggtgacgacg cccgggctcg cgccgtcgcc gagctgtgca
     6601 cacagcaggg tgagcatggg gcgcactcgc ttgccgcccg cctccaagag ataacggctg
     6661 gtgacgtcgg cgaggctgtc ggtgaagcgc acct
//
LOCUS       DASTNL010000141         5478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_839451, whole genome shotgun sequence.
ACCESSION   DASTNL010000141 DASTNL010000000
VERSION     DASTNL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839451"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFQ96_04420"
     CDS             complement(<1..759)
                     /locus_tag="VFQ96_04420"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0257065.1"
                     /translation="MAEIDIKPRSRTVTDGIEATTSRGMLRAVGMGDEDWDKPQIGVA
                     SSWNEITPCNLSLDRLARAAKEGVHAGGGYPLQFGTISVSDGISMGHEGMHFSLVSRE
                     VICDSVETVMMAERLDGSVLLAGCDKSIPGMLMAAARLNLASVFMYAGSIAPGWVRLS
                     DGTDHIITIIDSYEAVGAYNAGKMCKEDLHRIECGFAPGEGTCGGMFTANTMASVAEA
                     LGMSVPGSAAPPAADRRRDYFARRSGEAVVGMLRK"
     gene            complement(852..1646)
                     /gene="otsB"
                     /locus_tag="VFQ96_04425"
     CDS             complement(852..1646)
                     /gene="otsB"
                     /locus_tag="VFQ96_04425"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU0257066.1"
                     /translation="MIAEDGGLDEALASLTRVPRLLVALDFDGTVAPLVEDPATARPT
                     PAAREAVARLAGLPATSVAYVSGRQLSELGWLTGADDAVVLVGSHGAETSAGPRDAFA
                     LSAPERRRLDALRQAVARVHDRFPRARIEQKPAGVGVHVRGLETGLGHAVFGATRAAV
                     EKVVPEVGAYVIREGKGILEFAVRDVTKADAIRSLRASTRADAVFFAGDDVTDEDGFQ
                     ALEGAADVGVKVGEGVSAAPFRVADPAALGALVARLAVLRAAWAAA"
     gene            complement(1643..3154)
                     /locus_tag="VFQ96_04430"
     CDS             complement(1643..3154)
                     /locus_tag="VFQ96_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0257067.1"
                     /translation="MLSNRLPVHRIEEDGKTLWRTSPGGLVAALEPVMQAHGGVWIGW
                     PGFTDEPLDPFRFDGIDLVPVPLSDRELREYYEGFSNDTLWPLYHDVIAPPSYHREWW
                     GAYTSVNHRFAEAAAQAAAEGATVWVHDYQLQLVPLLLRQMRPDLVIGFFDHIPFPPS
                     GIFAQLPWRRQIVEGLLGADVVGFQRAADASNFSRAVRSLKGYDTHGSIIEVPLDGGG
                     PVSGGHRKSVDRHGNPVRTVLAKQFPISIDTATFTDLAARPEIQERARQIRHDLGDPA
                     TVMLGVDRLDYTKGIRHRLKAYGELLRDGRVAAEDVVLVQVASPSRERVETYRQLRDD
                     VELTVGRINGEFATIRHSAVSYLHHGYPREEMVALYLAADVMLVTALRDGMNLVAKEY
                     VASRIDNDGVLVLSEFAGAADELRQAILINPHDIEGMKERIMTAITMPRADRVKRMRT
                     LRRRVLANDVSRWSRSFLDALATTAGPEEPAQPGPEVPPVARLGRSQGGGSGA"
     gene            3424..3636
                     /locus_tag="VFQ96_04435"
     CDS             3424..3636
                     /locus_tag="VFQ96_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257068.1"
                     /translation="MRKYVSVAMLGVVFGLFRLLRRTARGPRDKRLALSWLGWGVGTS
                     LTCGSIRARSRRVARRPGVETAPRQP"
     gene            complement(4155..>5478)
                     /locus_tag="VFQ96_04440"
     CDS             complement(4155..>5478)
                     /locus_tag="VFQ96_04440"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU0257069.1"
                     /translation="ARLLLPALGQLLTEQPERRVELVGAGVENWDDARWRETVSHSFA
                     TTGSHGPAVEALVKATHYRPTDVTSTDQLRTLLASCSNAPALYFALPPAVTAKACDAL
                     AGVDLPEGTRFAMEKPFGSDVASAKALNARVLSLVPEDRVHRVDHFLANSTVLNLLGL
                     RFANRIFEPLWNAENIERVEIVYDEELALENRARYYDKAGALIDMLQSHLLQVMGVFA
                     MEPPATLGARDIRDNKAAVIRAAHVWHDDVAASSRRARYEAGTIGTRTLPAYIDEAGV
                     DPSRRTETLAEITLGIENWRWAGVPFTLRSGKALGEARHDITVTFRAANHIPEGLLGG
                     EEPTRLCFRMGPDSMSLDVNVNGPGDPYTLDRRSLQADFGAGALKAYGEVLAGIFDDD
                     PTLSVRGDTAEECWRIVEPVLAAWRRGDVPLDGYAAGSAGPEAWAPLP"
BASE COUNT          837 a         1888 c         1920 g          833 t
ORIGIN      
        1 cttccgcagc atgccgacga cggcctcgcc gctgcgtctg gcgaaatagt cgcgccgacg
       61 gtcggcagcg ggcggggccg ccgagccggg caccgacata ccgagcgcct cggcgacgga
      121 ggccatcgtg ttggcggtga acatgccacc gcaggtgcct tcgcccggcg cgaagccgca
      181 ttcgatacga tgcaagtctt ctttgcacat cttgccggcg ttgtaggcgc cgaccgcctc
      241 gtatgagtcg atgatcgtga tgatgtggtc ggtgccgtcg gaaaggcgca cccagccggg
      301 tgcgatcgag ccggcgtaca tgaagacact ggcgaggttc agcctggccg ccgccatgag
      361 catgccgggg atcgacttgt cgcagccggc gagcagaacg gagccgtcga gacgctccgc
      421 catcatgacg gtctcgaccg aatcgcagat gacctcgcgg gagacgagag agaagtgcat
      481 gccctcgtgg cccatcgaga tgccgtcaga gaccgaaatg gtgccgaact gcagcgggta
      541 gccaccaccc gcatgcacgc cctccttggc ggcgcgggca aggcggtcga gggagaggtt
      601 gcaaggcgtg atctcgttcc acgaactcgc caccccgatc tgcggcttgt cccagtcttc
      661 gtctcccatg ccgacggccc gcagcatgcc acggctcgtc gtcgcctcga tgccgtccgt
      721 tacggtgcgg cttcgcggct tgatgtctat ttcggccatg ccttcgagtc tatgacctcg
      781 ggcgtcgcgg cccagcggca ggagaacgcc cccaccgttc ggcgcaggac ggcgcgaccc
      841 gcagtcttcc ttcacgccgc ggcccatgcg gcacgcagca cggcaagccg ggccacgagc
      901 gcgccgagcg ctgcgggatc ggcgacacgg aacggcgccg ccgagacgcc ctcccccact
      961 ttgaccccga cgtccgccgc accctccagc gcctggaagc cgtcttcgtc cgtgacgtcg
     1021 tcgccggcga aaaatacggc atcggcccgg gtgctggcgc gcaggcttcg gatcgcgtcg
     1081 gctttcgtga cgtcacgtac ggcgaattca aggatccctt tgccctcccg aatgacgtac
     1141 gcgccgacct ccggcaccac cttctcgacg gccgctcggg tggcgccgaa gaccgcgtgc
     1201 cccagcccgg tctcgaggcc gcgcacgtgc accccgaccc cggcaggctt ctgttcgatg
     1261 cgggcccgcg gaaaccggtc gtggacacgt gcgaccgcct gccgcagcgc gtcgagcctg
     1321 cgccgctccg gcgcactcaa ggcgaacgcg tcgcgcgggc cggcagaggt ctcggcgccg
     1381 tgcgagccga cgagcacgac ggcgtcgtcg gcgcccgtca gccagccgag ttctgagagc
     1441 tgccggccgg agacgtaggc cacgctcgtc gcgggcaggc cggccaggcg ggcgaccgct
     1501 tcgcgggcgg ccggggtggg cctcgccgtc gccgggtcct cgacgagcgg ggcgacagtc
     1561 ccatcgaagt cgagggcgac gagcaggcgc ggcacgcgcg tcagagaggc aagcgcctcg
     1621 tcgaggcccc cgtcctcagc aatcacgccc ccgagcctcc accttgcgac cggccgagcc
     1681 gtgcgaccgg cggcacctcg ggcccgggct gcgcaggctc ctcgggcccg gcggtcgtcg
     1741 cgagcgcgtc gaggaaggag cgcgaccagc gggagacgtc gttggccaac acccgccggc
     1801 gcagcgtgcg catccgtttc acccggtcgg cacgcggcat tgtgatcgcc gtcatgatgc
     1861 gctccttcat gccttcgatg tcgtgcgggt tgatgaggat cgcttgccgc agctcgtcgg
     1921 cggcgcctgc gaactcgctc agcaccaaga cgccgtcgtt gtcgatgcgg gaggcgacat
     1981 actctttggc gacaaggttc atgccgtctc gcagggcggt gacaagcatg acgtcggcgg
     2041 caaggtagag cgccaccatc tcttctcgcg ggtagccgtg gtgcagatag ctgaccgcgg
     2101 aatgccgaat cgtcgcgaac tccccgttga tgcgcccgac ggtcagctcg acgtcgtcgc
     2161 gcagctgccg gtaggtctcg accctctctc ggctggggct cgcgacctga acgagcacca
     2221 cgtcttcggc ggcgacgcgg ccgtcgcgca acagctcgcc gtaggccttc aggcgatgcc
     2281 ggatgccttt cgtgtagtcg aggcggtcga ccccgagcat gacggtggcc gggtcgccga
     2341 ggtcgtgccg aatctgccgc gcgcgctcct gaatctcggg ccgggcggcg agatcggtga
     2401 aggtcgccgt gtcgatcgag atggggaact gtttggcgag cacggtgcgc acggggttgc
     2461 cgtgccgatc cacgctcttg cgatggccgc cggagaccgg gccgccgccg tcgagcggca
     2521 cctcgatgat cgacccgtgc gtgtcgtaac ccttcaagga gcgcaccgcg cgagagaagt
     2581 tgctggcgtc ggcggctcgt tgaaacccga cgacgtcggc accgagaagg ccttcgacga
     2641 tctgtcgtcg ccacggcagt tgggcgaaaa tgccggaggg tgggaacgga atgtggtcga
     2701 agaacccgat gacgaggtcg ggcctcatct gccgcaacaa cagaggcacg agctgcagct
     2761 ggtaatcgtg cacccagacc gtcgcgccct cggccgcagc ctgggccgca gcctcggcga
     2821 agcggtggtt gacgctcgtg taggcgcccc accattcgcg gtggtagctg ggcggcgcga
     2881 tgacgtcgtg gtagagcggc cacagcgtgt cgttggagaa gccctcatag tattcgcgca
     2941 gctcccggtc gctgagaggc accggaacaa ggtcgatgcc gtcgaaccgg aacgggtcga
     3001 gcggttcgtc ggtgaacccc ggccacccga tccagacgcc gccgtgagcc tgcatgaccg
     3061 gctcgagtgc ggcgacgaga ccgccggggg aggtgcgcca cagggttttg ccgtcttcct
     3121 cgatgcgatg caccggcagc ctgttcgaga ggatgacgaa cccgtaccca cccgagcgcg
     3181 accgcgtgac gacggaacgg cccgccgaag accgcgtgga acccggcccg tcaggctgcg
     3241 gccggccggc acccctggcg ggcggggcag actgaccggg cgacgtgggc ttcttctgcg
     3301 ctgacgttga aatggtcccg tccacctctc cgggtcgccc gaccgttgca cgcaggctac
     3361 cagccgccgg ataccggggc gcggtcaggc cgccgaccgc gtagcgtgtc cgtggaggaa
     3421 aagatgcgta aatacgtcag cgtggcgatg ctgggcgtcg tgttcgggct cttccgactc
     3481 cttcgccgca ccgcccgagg gccgcgcgac aagcgccttg ccctgtcgtg gctcggttgg
     3541 ggtgtcggca cctcgctcac ctgcgggtct atacgcgcca ggtcgcgccg cgtcgcaagg
     3601 cgaccgggcg tcgagaccgc gccccgccaa ccttgatgcc gggtcgcggc caggccatag
     3661 ccggcccgtg aagcgacccg cggacgaatt gtcggctgcc gggccgtatc tggcgcaaga
     3721 ccttatctgg cgcaagagcc gggcggcgtg tcttcagccc ctgcccatgc cgcccgggga
     3781 tgatgagggt acggcgcgtt gtcggtcgta ccggccggcg accgtcacgt ccgtcgcgac
     3841 gcgggcgggt gacggcgaga cccgaaagga gcgccgcaga tgttcggtct tcgcaggcgg
     3901 cacgtcgctg agataccggc cccgacacca cggctggacg atgctgcgct ggcgggggcg
     3961 gtgtatcgcg cgctggccga gttcttcggc ccggccggcc gctggcatct cgtgctccgg
     4021 cgggaggacg acaccgacgc cctgttccgc gcgctcatca ccgagtcgat cacggcgcgc
     4081 gtggtcggag acatccgcgc ggcgcagtct gatatcggcg ccgacacccc acccggcgcg
     4141 ccggggccac gggctcacgg cagcggcgcc cacgcctccg gcccggccga gcccgccgca
     4201 tacccatcga gcggcacgtc accgcgccgc cacgcggcca gcacgggctc gacgatccgc
     4261 cagcattcct cggccgtgtc accccgcacc gacagcgtcg ggtcgtcgtc gaagattccg
     4321 gccagcacct cgccgtaggc cttcaatgcc cccgcgccga agtcggcctg cagactgcgg
     4381 cggtcaaggg tgtaagggtc accgggaccg ttcacgttca cgtcgagcga catgctgtcg
     4441 gggcccatgc ggaagcacaa ccgggtgggc tcctcgccac ctagcaaccc ctcggggatg
     4501 tggttcgccg cacggaaggt gacggtgatg tcgtgccgcg cctcaccgag cgccttgccg
     4561 gagcgcaacg tgaacggcac gccggcccat cgccagttct cgatgccgag cgtgatctcg
     4621 gcgagggtct cggtgcggcg ggaagggtcg acgccggcct cgtcgatata tgcgggcagc
     4681 gtgcgggttc ctatcgtgcc ggcctcgtag cgtgcccgac ggctgctcgc cgcgacgtcg
     4741 tcatgccaga cgtgggccgc gcggatgacg gcagccttgt tgtcgcggat atcgcgggcg
     4801 ccgagcgtcg cgggaggctc catcgcgaag acgcccatca cctgcagcag gtggctttgc
     4861 agcatgtcga tgagcgcccc ggctttgtcg tagtaccgcg cacggttctc tagggcgagc
     4921 tcctcgtcgt agacgatctc gacgcgttcg atgttctctg cgttccacag cggctcgaag
     4981 atgcggttcg cgaagcgcag tccgagcaga ttcagaacgg tcgagttcgc caagaaatgg
     5041 tcgacgcgat gcacccggtc ttccggcacg agcgagagga cgcgcgcgtt caacgccttc
     5101 gccgacgcca catcggagcc gaacggcttc tccatcgcga aacgggtgcc ctcgggcagg
     5161 tccacgccgg cgagcgcgtc gcatgccttg gcggtgacgg cgggaggcag ggcgaagtag
     5221 agcgcaggag cgttactgca ggaggccaga agcgtcctca gttggtcggt actcgtgaca
     5281 tccgtcggcc gatagtgggt cgccttgacc agcgcctcga ccgcggggcc atgcgatccc
     5341 gtcgtggcga aagagtggga gaccgtctcg cgccagcgcg cgtcgtccca attttcgacc
     5401 ccggcgccga ccagctcgac ccgccgttcc ggctgctcgg tgagcagctg gccgagtgcc
     5461 ggcaagagca ggcgggcg
//
LOCUS       DASTNL010000142         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1210146, whole genome shotgun sequence.
ACCESSION   DASTNL010000142 DASTNL010000000
VERSION     DASTNL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210146"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFQ96_04445"
     CDS             complement(<1..816)
                     /locus_tag="VFQ96_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0257070.1"
                     /translation="MRTVLGRDPRSGACLAVTVTDGRVRLVEPAATTTTEGLPWVSPG
                     LTDLQVNGFHGHELNDPSLSTEAIDALRRALRAEGVTTFVPTVISGSRDHTAAILRAL
                     ARARRQSTALREAIPYVHLEGPYISAEEGPRGCHDPRHIRRPDADEFAAWQEQAEGAI
                     GMVTLSPHWAEAIDFTARLHRAGVRVSIGHTHAGPERIAEAVAAGARFSTHLGNGAHA
                     TLPRHPNYLWAQLADRRLTAGFIADGHHLDGATLTAMLRAKGLAHAFLVSDVTS"
     gene            complement(857..1642)
                     /locus_tag="VFQ96_04450"
     CDS             complement(857..1642)
                     /locus_tag="VFQ96_04450"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU0257071.1"
                     /translation="MFSHSTDAVARGTVGLLRYGVWGSPQALARHSAADIASEIISRL
                     SAKDDIRIVFAAAPSQQGTLSLLKDDPRIDWSRIHALHMDEYVGLAAGAPERFGVWLG
                     DHLFDHVPMGTVSLLDPRATDDPDAEARRYGELLTKKPIDIVLAGVGVNGHVAFNDPP
                     AHFAEPLPVRVVALSQASRQQQVTEGLFASLGDVPTHAWTVSIPVLMSAQAVFCMAPG
                     GLKSDAVTNMLTGPVTGEHPASVLRNHPHAALYLDTRSGRGLL"
     gene            2283..>3166
                     /locus_tag="VFQ96_04455"
     CDS             2283..>3166
                     /locus_tag="VFQ96_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0257072.1"
                     /translation="MSVMAIAAVALAGCSSGSGASSPSSSASAKGGIPATTITVMAAD
                     YGTGPTSANYSGKWWAKVVKAFNKKYPQVTVKMDVIPWGGAAGIDAKITAAVQAGNPP
                     DIAQGSADWAGLQSQVYPAKDVLTPAVLNDLTPIFAKQGDIGGTAYGIPWVASSRALV
                     YNKDLFAKAGISAPPTSWAEYKADAQKLKSAGVQTPACVPFGAEEAQAEFLIWTLGND
                     GGYVNAAGKWAIDSPQNVETFKFLNQLKTAGLIESSTGTTNRTSGSNSCWNQFDAGHV
                     GMTNSQNAQIPMLKSAGV"
BASE COUNT          549 a         1046 c         1083 g          488 t
ORIGIN      
        1 actcgtgacg tcggagacca agaaggcgtg cgcgagcccc ttcgcccgga gcatcgcggt
       61 caaggtggcg ccgtcgagat ggtgcccgtc ggcgatgaac ccggccgtga ggcgtctgtc
      121 ggccagttgc gcccacaagt agttgggatg ccgggggagc gtggcgtgcg cgccgttgcc
      181 caggtgcgtg ctgaagcggg cgcccgccgc gacggcctcg gcgatccgct ccggtccggc
      241 gtgggtgtgg cctatcgaca cccggacgcc ggcccggtgt agacgggcgg tgaagtcgat
      301 cgcctccgcc cagtgcgggg agagcgtgac catgccgatg gcgccctccg cctgttcctg
      361 ccacgcggcg aactcgtcgg cgtcggggcg gcggatgtgg cgtggatcgt ggcatccccg
      421 tggcccctcc tcggccgaga tgtaagggcc ttccaggtgg acgtacggta tcgcctcccg
      481 taacgccgtg ctctgtctcc tcgcccgtgc gagggcccgg aggatggcgg ccgtgtgatc
      541 tctcgaccca ctgatgacgg tcggcacgaa ggtcgtgaca ccttcggccc tgagcgcccg
      601 gcgcagcgcg tcgatcgcct ctgtggagag gctcggatcg ttcaactcgt ggccgtggaa
      661 gccgttgacc tggagatccg tcaggccggg cgagacccac ggcaggcctt cggtcgtcgt
      721 cgtggccgcc ggctcgacca ggcgcacccg accgtcggtc actgtgacgg cgaggcaggc
      781 cccggaacgc gggtccctgc cgagaaccgt tctcatgctc gccagcgttg ccccgggctc
      841 tcggccggtc gtgtggtcac aggagccccc gaccggatcg cgtgtcgagg taaagggccg
      901 cgtgcgggtg gttccgcaga acgctggcgg ggtgctcgcc tgtcacgggg cccgtcagca
      961 tgttcgtcac ggcgtcgctt ttgaggcctc ccggtgccat gcagaagacc gcctgggccg
     1021 acatgagcac cgggatgctg accgtccacg cgtgggtcgg cacgtcgccc agagaggcga
     1081 agaggccttc ggtcacctgt tgttgcctgc tcgcctggct caatgcgacg acgcgcaccg
     1141 gcaggggctc cgcgaagtgc gcgggcgggt cgttgaaggc cacgtggccg ttcacgccga
     1201 cgcccgccaa cacgatgtcg atcggcttct tcgtgaggag ctcgccgtac cggcgggcct
     1261 ccgcgtcggg gtcgtccgtc gcccgcgggt cgagaaggct caccgtgccc atcgggacgt
     1321 ggtcgaaaag gtggtcaccc agccagacgc cgaacctctc tggcgcgccg gcggcaagcc
     1381 ccacatattc gtccatgtgg agagcgtgaa tacgcgacca gtcgatgcgc gggtcgtctt
     1441 tcagaaggga taaggtgccc tgctggctcg gcgccgcggc gaacacgatc cgaatgtcgt
     1501 cttttgccga gaggcggctt atgatctccg aggcgatgtc tgccgccgaa tgccttgcca
     1561 acgcctgcgg cgagccccag acaccgtagc gtaacaggcc tacggtgcct cgcgcgacgg
     1621 cgtcggtgga atggctgaac atatgccccc tcgacgtgac ctgtctgcat ggtaggcgtc
     1681 ttcgcgcggt gagcgacccg acatgccgca ggtgaaaaat aatttgcaag gacactttac
     1741 aatctggtct gatcggtcta tacctgagga atatcagacg gcgagacgcc aacaccggaa
     1801 gggagggcgg ccttcggctc aggcatgaga gccgcgtgcg agaacggcgc cggccgtgac
     1861 cgcggggcgt cttgcaacgg gtagcgcatg gtgggaatcg cgaacaatca ctcagacagg
     1921 gtcgtcctgg gacgccggag gcgactcggc ccgaagagga gcccgcggac gggtgcgacc
     1981 tgcgtgtcgc acgcatccgg atgcgcccca cgtacctcgc gcgctcccca ccgatcctcc
     2041 gggccgcagg aacacgatgc cggcttcggc aggcgtcgcc cacctagccc cgtgatgccg
     2101 tcgacatgcc ccggtcggtg tctccgactt ctgcactgcg ggagaccgcg tgtaaagcca
     2161 cgcaggcggc gcgggggcac cgaagacgca cgaagcaatc agaaacacag cgcaaagcag
     2221 cgcatcgatc cgagaaaggg acaacttcga tgcaacacat gaagcggcat atcctcccgg
     2281 cgatgtcggt gatggcgatc gccgccgtcg ccctcgccgg atgctcctcc ggcagcggcg
     2341 cctcgtcacc ctcgtcgtcg gcgtcagcca aaggtggtat ccccgccacg acgatcaccg
     2401 tcatggctgc cgactacggc accggcccga cctccgcgaa ctacagcggc aagtggtggg
     2461 cgaaggtcgt caaagccttc aacaagaaat acccgcaggt gacggtcaag atggatgtca
     2521 tcccgtgggg cggcgcggcg ggcatcgacg cgaagatcac ggcggccgtg caggccggca
     2581 acccgccgga catcgcgcaa ggctcggccg actgggcagg cctgcaaagc caggtctacc
     2641 cggccaaaga cgttctcact ccggccgtgc tgaacgacct caccccgatc ttcgccaagc
     2701 aaggcgacat cgggggaacg gcctacggaa tcccgtgggt cgccagttct cgcgccctcg
     2761 tctacaacaa agacctcttc gccaaggcgg ggatctccgc gccgccgacc tcctgggccg
     2821 aatacaaggc cgatgcgcag aagctgaaga gtgccggcgt gcagaccccg gcctgtgtgc
     2881 cattcggggc tgaagaggcg caggccgagt tcctcatctg gacgctcggc aacgacggtg
     2941 gatacgtcaa tgcggccggc aaatgggcca tcgacagtcc acagaatgtc gagacgttca
     3001 agttcctcaa ccagctgaag acggcgggcc tcatcgagtc cagcacgggc acgacgaacc
     3061 gcaccagcgg gtcgaacagc tgctggaacc agttcgatgc cggccacgtg ggcatgacga
     3121 acagccagaa cgcgcagatc ccgatgctga agtcggcggg cgtgag
//
LOCUS       DASTNL010000143         7120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_99039, whole genome shotgun sequence.
ACCESSION   DASTNL010000143 DASTNL010000000
VERSION     DASTNL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99039"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="VFQ96_04460"
     CDS             <1..1697
                     /locus_tag="VFQ96_04460"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEU0257073.1"
                     /translation="GDIYWFASAVCGLIVGSSFRPPARLGRWARSSHHEIRVLVSSVV
                     AVTAVGPLVAILSPTRFGVLAPVALLTDGGVDPDCGRQAFTGHCLQDFALDPMRHHTI
                     VLDSLIPVVVLLVAAYYLLRGRRAALWVAVVFNGALGLSSLVSFILLPIVRGTGRQAS
                     LATHSWPGHVALAASAITPLAAAVLLVVLRRHFALGPTLDRVRRLLLGGTGAWHHGNP
                     AQGTLPGLEDGRARARRLLARDGGSLSHIATWGGNSYWFTDRADAGLAYRVVGGVALS
                     AGGPFGRPAAYEEAVRGFARFCDDRGWRPVVYSADARVLQPVARAMGWRTMVVAEEAV
                     LDPVTWSTRGKKWQDVRTAVNRAKKAGVTAVWTRFGALGLVEQTQIAEISEQWVAGKS
                     LPEMSFTLGGLGELADAEVRLMLAVGPDGRVEAVTSWMPFSREGAVVGWTLDVMRRRR
                     DAMNGVMEFLIEQTMERAREEGLAEVSLSGAPLARVVQGPPTPLDRTLERIGSVFETR
                     YGFASLMRFKSKFQPCYRPLVMAYPDPALLGTIGMAVLRAYLPTLSMRQAMRLLRS"
     gene            1749..3020
                     /locus_tag="VFQ96_04465"
     CDS             1749..3020
                     /locus_tag="VFQ96_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU0257074.1"
                     /translation="MATPFAEATALMVLVAVLLFAVVRPGRLPEAVAAVPGAALLLLT
                     GVAPAARAWREASGVTPTVVFLAAVLMLAQLCDAEGLFRAASAWMSRRAAGRASRLLT
                     LVFVVVSLTTAVLSLDATVVLLTPVVFQTADRLGVRARPHLYATTHLANAASLALPVS
                     NLTNLLALGAAGVGFVRFAGLMAGPWLVAIAVEYVVFRWRFRADLAVVARPHDEARTA
                     APMPVFAVVVLGLTLAGFAASSPLGLPPFWVALAAVVALLVKRAVRTPRGLAAQLART
                     ARAAHVSFLVFVVALAVIVGSVVDHGLADGLRRLLPAGDGLVSLLVVAALAAVLANVV
                     NNLPAVLVLLPLVAPAGLLQVLAVMIGVNLGPNLTYVGSLATLLWRRIAVGRGHAVEL
                     REFTRLGLLTVPAGLFFCTCALWLGARLWGA"
     gene            complement(3125..4234)
                     /locus_tag="VFQ96_04470"
     CDS             complement(3125..4234)
                     /locus_tag="VFQ96_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0257075.1"
                     /translation="MAAAIGAAVLTASLLTAGAADAAPPSPACVSNAGGTGLSAAVVA
                     KAHQTISNRTIRTNCDIGIYVGQGATAVRIKHVTVSGAHYQGIFAEKTSHLLIDHSSV
                     HGNGFQTVDPSAPALEGSGVHSYVGEAFGISLFGVSDSFIWDNSVYHNGRGGIGIMDN
                     GANDPGLNTKTASQNKSAPLISSTNDVITGNRMWGNYTGCGLVAATQNLGGSLSNLVL
                     SNNTITGTGLSKTRGPDVGGLIVAANTPGSKVSNVSVSGNKVTGSAEGGIVVNAEAPT
                     SPAGAGAATSNVWITNNTVTRNNLFGLTPEPKTTVGVVVFSGGPGATNTGTVVAQNRI
                     SQQNYGIWSNGSTTPTTLFNQISVTRGGTDIYHQG"
     gene            4674..5867
                     /locus_tag="VFQ96_04475"
     CDS             4674..5867
                     /locus_tag="VFQ96_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEU0257076.1"
                     /translation="MDTAMMKKPRVTRRMRLAVFGMALGALVLGGLSPAAPASASTSG
                     LSASGFKASVHGAKVTVKVKVSSAKTRKAVRAGICARSSTHRNVDFSASRNVNITPHG
                     TTFTKVKTFAPGRYTYAACAEFGHTWKTSDWKHTFTVKKVAKSASVAVKPASAATVIV
                     ASAAAAASSTAMPVGDLPGWHQVLAQNFTRDTALGDFPGPYTGQWMSYNGFGDTSGIG
                     LYDQSIISSHNDELDLYVHTADGRPLGAAPIPLVDGQWGGQRYGMFSVRMKADALAGY
                     GAAFLLWPDSNNWNQGEIDFPEGALNGTVSAFNHCPGHPQDTCLAAATKTNFTAWHTY
                     TIEWTPTKLSFLIDGKVIRSTTKNIPQGVMHWVGQVGTNGVKPAASVKGHVFIDWMTI
                     YTYRP"
     gene            complement(5874..>7120)
                     /locus_tag="VFQ96_04480"
     CDS             complement(5874..>7120)
                     /locus_tag="VFQ96_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257077.1"
                     /translation="SGSRAWLAGAIFALTETLNIVYFSPQSIGILISLVIFALAVSPR
                     RIGATPRRSGADPMPRPHHRLAAAYAPLGAGRLVFILYLSCVSAVTHQISPYLTVAAL
                     ALLWIMGYLRPVWTLLLIIAPALAWAGANTDVLGRFISLGAVGRFWENAQSPAHTDTQ
                     FATPLVTRLAFEIPAVVLLALGLAAAYAVLRQRTRPAWALALAGASPVTLFAVTNYGQ
                     EGIFRVTLFAAPWLAVLAAAIPWPAPARRWRTPALAAGLAVLLGANVFGQTALDWNRV
                     TSQDTARATALYENTAPRGSLLLLTGTSVVSPINISARYLDVTYVGRESLGGFPPPDR
                     PYDAAADVATLTRRLVTQWPASGYYALVSETIGAYDARYGYQNYAEYRKLAAAMAASP
                     LWRPIFHGPTTTLYVLAKKPAG"
BASE COUNT          978 a         2318 c         2540 g         1284 t
ORIGIN      
        1 ccggcgacat ctattggttt gcctcggcgg tgtgcgggct catcgtcggc tcgtccttcc
       61 gcccgccggc gcggctgggg cggtgggctc gcagctccca ccacgagatc agggtgctcg
      121 tctcctcggt ggtcgcggtg accgccgtgg gtccgctcgt cgccatcctc tcgccgacac
      181 ggttcggggt gctcgccccg gtggcgttgc tcactgacgg cggcgtcgac cccgattgcg
      241 ggcggcaggc gttcaccggg cactgcctgc aagacttcgc gctcgaccct atgcggcatc
      301 acacgatcgt gctcgactcg ttgattccgg tcgtcgtgct gctcgtggcg gcgtattacc
      361 tgctgcgcgg taggcgggcc gccctctggg tcgccgtcgt gttcaacggt gctctcggtc
      421 tgtcgtctct cgtctcgttc attctgctgc ccatcgtccg cggcaccggt cggcaggcaa
      481 gccttgcgac ccacagctgg ccgggtcatg tcgcactcgc cgcctcggcg atcactccgt
      541 tggcggcggc ggtgctcctc gtcgttctgc ggcggcattt cgccttgggc cccacgcttg
      601 accgcgtgcg ccggcttctt ctgggcggca ccggtgcgtg gcatcacggc aacccggcgc
      661 aggggacgct gccgggactg gaggatggcc gtgcccgggc gcggcggctt ctcgcgcgtg
      721 atggcggctc cctgtcgcat atcgccacct ggggcggtaa ctcgtactgg ttcaccgatc
      781 gtgcggatgc gggcctcgcc taccgggtcg tcggcggcgt cgccttgagc gccggcggcc
      841 cgttcggacg gcccgcggcg tatgaggagg ccgtgcgggg ttttgcccga ttctgcgacg
      901 acaggggctg gcgccccgtg gtctacagcg ccgacgcccg tgtcttgcag ccggtcgccc
      961 gcgcgatggg ctggcgcacc atggtcgttg ccgaagaggc cgttctcgat cccgtcacct
     1021 ggtcgacgag aggcaagaag tggcaagacg tgcgtactgc cgtcaacagg gcgaagaagg
     1081 ccggggtcac ggcggtatgg acccggttcg gcgcgctcgg gctcgtcgag cagacccaga
     1141 tcgcggagat ctccgagcag tgggttgccg gcaagagcct gccggagatg tcgttcacgc
     1201 tcggcgggtt gggcgagctg gcagatgcgg aggtgcgtct tatgctcgcg gtaggcccag
     1261 acggccgcgt cgaggctgtc accagctgga tgccgttctc gcgggaaggt gccgtcgtcg
     1321 ggtggacact cgacgtcatg cgccgccgcc gggatgcgat gaacggcgtc atggagttct
     1381 tgatcgagca gacgatggaa cgtgcacgcg aggaggggct ggccgaggtg agtctctccg
     1441 gtgcgccgct cgcccgcgtg gtgcaggggc cgccgacgcc gctggatcgc accctcgagc
     1501 ggatcggttc ggtgttcgag acacgctacg ggttcgcgtc gttaatgagg ttcaaaagca
     1561 agtttcagcc gtgctaccgc ccgctcgtga tggcgtaccc cgatccggcg ctcctcggaa
     1621 ccatcggcat ggccgtgttg cgggcctacc tacccacgct ctctatgcgg caagcgatgc
     1681 gcctcctgcg atcgtgactc ggcgccgcct gagccggacg ggccgcccag gcctgttagt
     1741 gtgcgtcggt ggcgactcct ttcgccgagg ccacggcgct gatggttctg gtcgcggtgt
     1801 tgctcttcgc cgtcgtacgg ccggggcggc tgccggaggc ggtcgccgca gtgccgggtg
     1861 cggcgcttct cctcctgacg ggcgtcgcgc ctgccgccag ggcctggcgc gaggcttcgg
     1921 gcgtcacccc gaccgtcgtg ttcctggcgg ccgtcttgat gctcgcgcag ctgtgcgacg
     1981 ccgaagggct ctttcgggcg gcgagcgcgt ggatgtcacg ccgggcggcc ggcagggcct
     2041 ctcgcctctt gacgctggtg ttcgtcgtgg tgtcgctgac gacggcggtg ctgagcctcg
     2101 atgcgaccgt cgtgctgctc acccccgtcg tctttcagac ggccgaccgg ctcggcgtgc
     2161 gcgcccggcc gcacctgtat gcgacgacgc atctggccaa cgcggcgtcg ttggcattgc
     2221 cagtgtcgaa tctcaccaac cttctcgcgc tcggcgccgc gggcgtcggg ttcgtgcgct
     2281 tcgccggcct tatggccggg ccgtggctcg tggcgatcgc ggtggagtat gtcgtcttcc
     2341 gctggcgttt ccgagccgat ctggccgtcg tcgcccggcc ccatgacgag gcgcgcacgg
     2401 ccgcgccgat gcccgtcttc gccgtggtcg tgctggggct gacccttgcc gggttcgccg
     2461 caagctcgcc gctcggcttg ccgccgttct gggtcgcgct cgccgccgtc gtggcgctcc
     2521 tcgtgaagcg cgccgtccgc acgccgcgcg gcctcgccgc ccagctggcc cgtacggcgc
     2581 gggcggcaca cgtctcgttc ctcgtcttcg tcgtcgccct ggccgtgatc gtcggttccg
     2641 tcgtcgatca cggcctggcc gatggcctgc gccggctgct gccggccggc gacggtctcg
     2701 tcagccttct cgtcgtcgca gcgcttgccg ccgtgctcgc caacgtcgtg aacaacctgc
     2761 ctgccgtgct cgtgctgttg cctctcgtcg cgcccgccgg cctgctccag gtgcttgccg
     2821 tcatgatcgg cgtcaacctc gggccgaacc tgacctacgt cggctcgttg gcgacgctgc
     2881 tctggcggcg tatcgcggtg ggtcgcggcc atgccgtgga acttcgcgaa ttcacccgtc
     2941 tcggcctctt gaccgttccg gccggcctct tcttctgcac ctgcgcgctc tggctcggcg
     3001 cccgactctg gggtgcttga gtgcggcgct cgtcgccggg ggagcgggcg gcgagccgcc
     3061 ctggccgtgt gggcccgggg cggcacgccg cgtgtggtgc catattgcct tggcttggcg
     3121 acgcctaccc ctggtggtag atgtcggtgc cgcccctagt tacgctgatc tggttgaaga
     3181 gcgtcgtagg ggtcgtgctg ccattcgacc agatgccgta attctgctgg ctgatgcggt
     3241 tttgggcgac gacggtccct gtgttcgtgg ctcccggccc cccggagaag acgaccacgc
     3301 cgacggtcgt cttaggctcc ggggtcagcc cgaagaggtt gttacgggtg accgtgttgt
     3361 tcgtgatcca cacgttgctc gtggctgcgc cggcgccggc aggtgaggtg ggggcctccg
     3421 cgttcacgac gatgccgccc tccgcggagc cggtgacctt gttacccgag acggagacgt
     3481 tgctgacctt cgagccgggg gtgttggcgg cgacgatgag gcccccgaca tccggtccgc
     3541 gggtcttcga aagccccgtg ccggtgatcg tgttgttcga cagaacgagg ttagagaggc
     3601 tcccgcccag gttctgcgtc gccgcgacca ggccacagcc ggtgtagttg ccccacatgc
     3661 ggttgccggt gatcacgtcg ttcgtcgagc tgatcagcgg ggcggatttg ttctgcgagg
     3721 cggtcttcgt gttgaggccc ggatcgttgg cgccgttgtc catgatgccg atgccgccgc
     3781 ggccgttgtg gtagacgctg ttgtcccaga tgaacgagtc ggagacgccg aacaggctga
     3841 tcccgaaagc ttcaccgacg taggagtgca ctccgcttcc ctcgagagct ggcgccgagg
     3901 ggtcaaccgt ctggaatccg ttgccgtgta cggaggagtg gtcgatcaga aggtgggagg
     3961 tcttctcggc gaagatcccc tggtagtgcg cgccggagac ggtgacgtgc ttgatccgta
     4021 ccgcggtggc cccctggccg acgtagatgc cgatgtcgca gttggtgcgg atggtgcggt
     4081 tcgaaatggt ctggtgcgcc tttgccacca cggccgcgct cagccccgtg ccccccgcgt
     4141 tggagacaca ggccgggctc ggcggcgcgg cgtcggcagc gccggctgtg aggagcgacg
     4201 ccgtcaagac cgcggcgccg atcgctgccg ccatggcctt cttattcatg ttcactgcag
     4261 gtcacatccc ttttctgctt gttgccactg ttctgttgac aatcccgcgc gtctcgtcgg
     4321 tgagactctt gggcgctcgg gcgtgagcac aactttcctc cgattcggct ctcgccatga
     4381 tgtggattcg cgtagttttc tggcgccggt gacgcctttg tcaacgaagg ggtcggcagg
     4441 tcacgcgagc ctgtttttct cccgaagatt acgtgagcgg agaacgggtc gatagatcac
     4501 agagtgcgtc gcggagtctt ggcgctttcc gcacgcatgg cgcgacccga gaaacgtgcc
     4561 gacgagatgc gccccgggtg tgccgcgccc gttccccgcc gtgctgccac ggcgctgggg
     4621 gcaccgtgcg gccgccgacg gtgagcaaga ccaggcatgc gaaggagttg ccgatggata
     4681 cggcgatgat gaagaaacct agagtcacga ggcggatgcg cctcgcagtt ttcggaatgg
     4741 cgctcggcgc gttggtgcta ggcggcctct cgccggccgc acccgcgtca gcttctacct
     4801 ccggcttatc ggccagtggg tttaaggcga gcgtgcacgg cgcgaaagtc acagtcaagg
     4861 tcaaggtgtc gagcgcgaag actcgtaagg ccgtgcgggc gggcatctgt gctcggagtt
     4921 ccacgcaccg caatgttgac ttcagcgcca gccgcaacgt gaatatcacg ccgcacggca
     4981 cgaccttcac gaaagtgaag acatttgcgc cgggccggta cacgtatgcg gcgtgtgccg
     5041 agttcggaca cacgtggaag acctcagatt ggaagcacac gttcaccgtg aagaaggtgg
     5101 cgaagtctgc gtcggtcgca gtgaagcccg catcggccgc cacggttatc gtcgcgtcgg
     5161 ccgccgcagc cgcatcctcc accgcgatgc ctgtcggtga cctgcccggg tggcatcagg
     5221 tgctggcgca gaacttcacg agggacaccg cgttgggcga ctttcctggc ccgtataccg
     5281 gccagtggat gagctacaac gggttcggag atacgtcggg tatcggcctc tatgaccagt
     5341 cgataatctc gtcccacaac gacgaactcg acctttacgt gcacacggcc gacggccggc
     5401 cgttgggcgc cgccccgatc cccctcgtag acggccagtg gggcggccaa cgctacggca
     5461 tgttcagcgt gcggatgaag gccgacgccc tcgccggcta tggcgcggcg ttcctgctct
     5521 ggcccgacag caacaactgg aaccagggcg agatcgactt tcccgaaggg gcgctgaacg
     5581 gcaccgtctc cgcgttcaac cattgccccg gccacccgca agacacctgc ctcgcggccg
     5641 ccaccaagac caacttcacc gcatggcaca cgtacacgat cgagtggact ccgacgaagc
     5701 tgagcttcct catcgacggc aaggtgatca ggtcgacgac gaagaacatt ccgcaagggg
     5761 tcatgcattg ggtcggccag gtcggcacca acggcgtgaa gccggccgcc tcggtgaagg
     5821 ggcacgtgtt catcgactgg atgacgatct acacgtaccg gccgtagcgg aggtcagccg
     5881 gccggtttct tcgccagcac atagagcgtc gtcgtcggcc cgtggaagat cggccgccat
     5941 aagggcgacg ccgccatcgc ggctgcgagc ttgcggtatt ccgcgtagtt ctggtaaccg
     6001 taacgtgcgt cgtaggcgcc gatcgtctcc gagacgagcg cgtaataccc ggaggcgggc
     6061 cactgggtga ccagccggcg ggtgagcgtc gccacgtcgg cggccgcgtc gtacggccgg
     6121 tcgggcggcg ggaacccgcc gaggctttcc cggccgacgt aggtgacgtc gagataccgg
     6181 gcagagatgt tgatcggcga cacgacgctt gtgccggtca agagcagcag cgagccgcgg
     6241 ggtgccgtgt tctcatagag cgcggtggcg cgggccgtgt cttgggaggt cacgcggttc
     6301 caatccagcg cggtctggcc gaacacgttc gcgccgagca gcacggcgag tcccgccgca
     6361 agcgcgggcg ttcgccaccg acgggcgggc gccggccagg ggatcgccgc cgcgagcacg
     6421 gcgagccatg gcgcggcgaa cagggtgacg cgaaagatcc cctcctgccc gtagttggtc
     6481 acggcgaaga gggtgacggg gctcgcaccc gcgagcgcca gcgcccaggc gggccgtgtc
     6541 ctttggcgca gcacggcgta cgccgccgcc agcccgagcg ccagaagcac gacggcgggg
     6601 atctcgaagg cgagccgcgt gacgagcggg gtggcgaact gggtgtcggt gtgcgcgggg
     6661 ctctgggcgt tctcccagaa ccggcccacg gcgcccaacg agatgaagcg gcccaagacg
     6721 tcggtgttgg cgccggccca ggcgagcgcc ggggcgatga tgagaagaag ggtccagacc
     6781 gggcgcaggt agcccatgat ccagagcagg gcgagcgccg cgacggtgag atagggagag
     6841 atctggtgcg tcacggccga tacgcacgac aggtagagga tgaagacgag ccggccggcg
     6901 ccgagcggcg cgtaggcggc ggccagcctg tggtgcgggc gcggcatcgg atcggcgccg
     6961 cttcggcgtg gcgtggcgcc gatccggcgc ggtgagacgg cgagcgcgaa gatgacgagg
     7021 gagatcagga ttccgatcga ctgcggcgag aagtagacga tgttcagcgt ctcggtgagg
     7081 gcgaagatcg cgccggccag ccacgcccgg ctgccggagc
//
LOCUS       DASTNL010000144        15678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_285511, whole genome shotgun sequence.
ACCESSION   DASTNL010000144 DASTNL010000000
VERSION     DASTNL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 15678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15678
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285511"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFQ96_04485"
     CDS             <1..308
                     /locus_tag="VFQ96_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0257078.1"
                     /translation="LPRLSDAAELVLYRVAQESLTNVARHAHATRAAVSLTASADAVT
                     LRVTDDGPAGSFHFQEGAGIRGMRERALLVRARLDIGAAPEGGTEVLLRVPTATRGS"
     gene            313..978
                     /locus_tag="VFQ96_04490"
     CDS             313..978
                     /locus_tag="VFQ96_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0257079.1"
                     /translation="MPATSTTPIRILLADDHALVRKGLRLILDSEPDLRVVRAVDDGA
                     AAVEAVAAGDIDLAILDIAMPRMTGIQAAREISARTPQTRILILSMYDNEQYFFEALK
                     AGASGYVVKSAADRDLIEACRAMMRGEPFVYPGAIAALIRDYLRRDRQGERASSTLLT
                     PREEQITKLIAEGESTKQIAAALSVSVKTVERHRANILQKLGMRDRLDLTRFAIRTGL
                     IEP"
     gene            complement(1027..1512)
                     /gene="greA"
                     /locus_tag="VFQ96_04495"
     CDS             complement(1027..1512)
                     /gene="greA"
                     /locus_tag="VFQ96_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU0257080.1"
                     /translation="MSQETSVSFLTRDAYDRLAAELEELTTTGRVEIAKKIEEARAEG
                     DLRENGGYHAAKDEQGKIEARIATLTQLLRTASVGDAPENRGIVEPGLLITATIAGDE
                     TVFVLGSREIAGDTEISVYSEQSPLGSAILGLKVGDTTSYQAPNGKNIAVRIEKVDTF
                     A"
     gene            complement(1600..2019)
                     /locus_tag="VFQ96_04500"
     CDS             complement(1600..2019)
                     /locus_tag="VFQ96_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HEU0257081.1"
                     /translation="MPVQESEASEVQTRYGRVRKHRISEGWLIGGFAALAVIIFFVWA
                     IAVNGIGSTSSTLGSNVTGTSITHHGMSVTVRYTVSVTPGTAYSCAVEADSEDHAIVG
                     WKVVSFPATSAQQTAHEQTIRTVQPAVAGLISDCWVR"
     gene            2146..3006
                     /gene="mca"
                     /locus_tag="VFQ96_04505"
     CDS             2146..3006
                     /gene="mca"
                     /locus_tag="VFQ96_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEU0257082.1"
                     /translation="MTHRLMAVHAHPDDESSKGAATYAHYAAQGDEVMVVTCTGGERG
                     DILNENLSQLALARRDLPGLRRSEMAAAQAVMGVQHRWLGYVDSGMAREDGTVPTGSF
                     ADIPVEIEAEPLVRLIRSFQPQVLITYDENGGYAHPDHIRSHVVAMEAYRAAADPARY
                     PSAGEPWQIAKLYYERILNAPRIRKVYELLKRRQPDSPLIAKFETMRSWLDERPLQAT
                     TQVAVGDELDVRDAALRAHASQVAPDSPFFFWPNDVVREAWPFEDYQLVESQTSTPLP
                     ETDLFAGVEA"
     gene            3009..3320
                     /locus_tag="VFQ96_04510"
     CDS             3009..3320
                     /locus_tag="VFQ96_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257083.1"
                     /translation="MFTALTAGVQILADSSTPGDDNAQYYAHSPGAWAFFTLLGIGIV
                     VVLLCADMVRRMRRVRYRAEIAAKLDAEEAELLARPHTAEGGDGRGPSGRVSPAGPAT
                     P"
     gene            3344..4123
                     /locus_tag="VFQ96_04515"
     CDS             3344..4123
                     /locus_tag="VFQ96_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU0257084.1"
                     /translation="MTPRSSGRGPLYRIYQRRLRRSFDPGVLPRHVAMIVDGNRRWAR
                     LAGLDSVAPGHRAGAAKMKEFLAWCDRLGIGVVTLYLLSTDNLANRDAAELADLTTII
                     AELADDVSRVRGWRVQHVGSDAGLPPGLAAALRAAEERSAQGTGLHVNLAVGYGGRKE
                     ITEAVRSIIRDHHESGGSLETLAESLTPEMIGRHLYTRGQADPDLVIRTSGEQRLSDF
                     MLWQSAHSEFYFVEALGPDLREVDFLRAIRDYTRRKRRYGG"
     gene            4125..5222
                     /locus_tag="VFQ96_04520"
     CDS             4125..5222
                     /locus_tag="VFQ96_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEU0257085.1"
                     /translation="MVIDGYLSGFTEEPGYLDYARVGPLSAGVAAEVLANTEALRRSR
                     FGSLDAMVGEHDRLCAAVERAVGFPARNVVFQSNTSIALMQVLFALRGDVLLSPDEFP
                     SMTFAAARAASMGVCRPIWLDAERPVTAEAVLDVLSDEVTALVVSLVDYRTGYRVDLP
                     ALRQVIGGRLLIVDATQGLGVVDAAFHDADVVVSGGQAWLRAGWGSGFLAVSDHALGL
                     LDPALAGYPGAAEDPFTTVSGPDGTVMSFTVNEPDYAAAARLAAALEEVAGAGAAQIE
                     SLVQEKVEELIAAADTAGVPVASPRDAARRAGIVTLRPEPAAVTALAAAFFNRGVTVT
                     TQAGTVRLSVHAGTSDATLGMVRDALAVFAA"
     gene            5314..5952
                     /locus_tag="VFQ96_04525"
     CDS             5314..5952
                     /locus_tag="VFQ96_04525"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEU0257086.1"
                     /translation="MARIPDTRGLAPDSLEGWLLPCRAGLLPGQASAGAQGGRARRRL
                     AAGLAVALAAAALSAWALCWAGVGPRALLPLYLAGVSLPLCAWDLAHRRLPNALVLPG
                     YAVAAVGILGLRLAGEPAAPALAAGAGTFGFFLLLALAGGMGMGDVKLAGLIGLASGA
                     LAPGAALLAVVIAFLTGGVAGIAAWLVPELRTRGIPFGPYLLLGLWAAPAFA"
     gene            complement(5974..7377)
                     /locus_tag="VFQ96_04530"
     CDS             complement(5974..7377)
                     /locus_tag="VFQ96_04530"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU0257087.1"
                     /translation="MDDSNEFRIEHDTMGEVRVPAAALYGAQTQRAVENFPISGTRLE
                     RAQIAALARIKKSAARANERLGVLDAQLAEAIAHAADEVAEGRHDAEFPVDIYQTGSG
                     TSSNMNMNEVLATLASQSLGRPVHPNDHVNASQSSNDVFPTSVHVAVTAALTSDLIPA
                     LEHLAKEFEEKARQWAEVVTAGRTHLMDATPVTLGQEFSGYAAQMRLGVERVRASLTR
                     VSEVPLGGTAVGTGINTPAAFPAMVIELLKEETGLPISEARNHFEAQANRDALVEASG
                     ALRVIAVSLTKICNDLRLKSSGPNAGLGELHLPDLQPGSSIMPGKVNPVIPEATLMVA
                     SRVIGNDATIAFSGASGAFELNVSIPIMGTALLESIRLLTNAVRLLADKTIAGARADV
                     ARARALAESSPSIVTPLNKVIGYEAAAKIAKHAIADSVTIRQATIDLGFVERGEITLE
                     ELDARLDVLSMTHPAQS"
     gene            complement(7434..8072)
                     /locus_tag="VFQ96_04535"
     CDS             complement(7434..8072)
                     /locus_tag="VFQ96_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU0257088.1"
                     /translation="MTDLPTPAQAWQLLRDGNARYAADERTHPRQDAARRAELAFEQH
                     PFATLVGCGDSRVALEHIFDLGLGDAFVVRNAGHVLTESAIGSIEYGLGPLTTPILVI
                     AAHERCGAVRAAIDAQGAGAAALPPHIAATIAPIASAVHSVAGTAEGETLDPASVDAA
                     EVGKAHVRASIELLLTESDIARKALEAGTLAIVGAYYRLSGPVETVCTRGLD"
     gene            complement(8069..8731)
                     /locus_tag="VFQ96_04540"
     CDS             complement(8069..8731)
                     /locus_tag="VFQ96_04540"
                     /inference="COORDINATES: protein motif:HMM:NF025396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 family protein"
                     /protein_id="HEU0257089.1"
                     /translation="MSRTPKAPAIVAELGRPETPEEAALRREAATRERRSRRTIGNLI
                     VSLLAVAAAVVVVAMMTPTPKMTAAPRADFRSIAKQVSGAESTALVVPDLDSSWRCNV
                     ARLNTNTPDGIDYWQLGFVTPSDGFVTIEQGFDANPTWAANQLSPAAAAGHRTIDGIT
                     WNLFDNHTSAGSAVRYGLTVTYGKNAYAVFGSASLAEVEHVAGSISGQITATRLRSAP
                     TR"
     gene            complement(8775..9023)
                     /locus_tag="VFQ96_04545"
     CDS             complement(8775..9023)
                     /locus_tag="VFQ96_04545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0257090.1"
                     /translation="MATDTAEPGFEELSYEEARDELVKVVGLLEQGSATLEESLVLWE
                     RGEALAARCEKWLLGARERLDAVRGTHEAGAAASEGVE"
     gene            complement(9034..10275)
                     /gene="xseA"
                     /locus_tag="VFQ96_04550"
     CDS             complement(9034..10275)
                     /gene="xseA"
                     /locus_tag="VFQ96_04550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU0257091.1"
                     /translation="MTDALSTPDAPWPVHVLGEKLRGYIDRLGTAWVEGEITGWNVSA
                     GNVYGRLKDVDVDATVAFTIWSRTRSRIPDDLVRGARVVAQVKPNYWVKGGTLTMQVQ
                     EMRHVGLGDLLERIERLRRQLAAEGLFDAERKKRLPFLPRLIGLVTGRDSDAEKDVKR
                     NALLRWPAVRFRTAYAAVQGDRSVDEVVAALQRLDADPEVDVIIVARGGGDFQNLLSF
                     SDEKVVRAAALSATPVVSAIGHEADRPLLDEVADLRASTPTDAAKRVVPDVAEEILRV
                     QQARSRIGLRLTALVRGEAEALERLRTRPALASGDWIVDSRADELGRYMERSRDLVSR
                     TVERASAETARLRAQLRALSPQMTLDRGYAIAHKEGGAVVRSAAEAPAGTRLRLTFAN
                     GAIGATSTGAPQPRGRRAAER"
     gene            10416..11057
                     /locus_tag="VFQ96_04555"
     CDS             10416..11057
                     /locus_tag="VFQ96_04555"
                     /inference="COORDINATES: protein motif:HMM:NF040366.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6264 family protein"
                     /protein_id="HEU0257092.1"
                     /translation="MGDERERPRYGEYATPEEQQRSIQDPEARRRIARAASGQAADEG
                     PAAEGPGAGARPPRPASDHGLERARGSFAAPTGSHRGMPGVASADGRAPGRRWDLILT
                     LVLLAVGLLDVVTSVSSYLHLDQTLAAVYQRLGVHASPAAGDAATAGAALVAVRVGLW
                     VVAAGLAAWRLTARRLAFWIPLVAAAVSYVVIVVVMLEVVFQDPAVRTFLQNQ"
     gene            complement(11070..12098)
                     /gene="fbaA"
                     /locus_tag="VFQ96_04560"
     CDS             complement(11070..12098)
                     /gene="fbaA"
                     /locus_tag="VFQ96_04560"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU0257093.1"
                     /translation="MPVATPDQYAEMLDKAKKKGFAYPAFNVSSSQTIHAVLQGLTEA
                     GSDGIIQVTTGGADYFAGQTIKARAAGALAFAKFATEVAKNYPVTVALHTDHCPKPAL
                     DSFLLPLLAASEEEVKAGHEPIFQSHMWDGSAVPLDENIRIAQEMIARTSAIHTILEI
                     EVGVVGGEEDGVKHEGTNEALYTTVGDVSKAVDALGFGENGRYMAALTFGNVHGVYKP
                     GNVKLRPDLLGEIQAGIAKKYGKADKPLDLVFHGGSGSSPEEIATAVANGVVKMNVDT
                     DTQYAFTRAIAGYMFEHYAGVLKVDGEVGVKKQYDPRAWGKVAETAMAQRVIQAAQQL
                     GSAGHSGH"
     gene            12435..13367
                     /gene="glpX"
                     /locus_tag="VFQ96_04565"
     CDS             12435..13367
                     /gene="glpX"
                     /locus_tag="VFQ96_04565"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU0257094.1"
                     /translation="MELVRATEAAAIRAAPWIGKGQKNTADGAAVDAMRAFLGTVNFD
                     GVIVIGEGEKDHAPMLFNGEHVGNGRGPACDIAVDPIDGTSLTAYGHQNAVSVIAVSD
                     RGSMYDPSAVFYMNKIVCGFEGIGVVDIRKPIGENIRALAKAKGKHVSEMTVAILDRP
                     RHAQLIDEVRAAGAGTRLLLDGDVAGGINAARYASYIDMCVGVGGTPEGIITACAVKA
                     LGGLVQGQLMPKDDAERQKAIDAGHDLDRVLDQDDLVTGDNTYFVATGVTNGELVHGV
                     ERKGPVIRTESIVLRSKSGTIRRIVAEHLVDKWS"
     gene            complement(13383..14750)
                     /gene="rmuC"
                     /locus_tag="VFQ96_04570"
     CDS             complement(13383..14750)
                     /gene="rmuC"
                     /locus_tag="VFQ96_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU0257095.1"
                     /translation="MDIVLLVIGVALLAAVVALLAAVTRSMRGAGAAESPELVAERHA
                     NEIDRVRHEEAQERARLQQEDAERRAQLAERCAAAETSAQELRAQVQLLRDELEKVRA
                     GHKAEAEARQEQERRESKVLQALAPVRETLGLMQGKVTQMEAQRQAQYGALSEQLTQA
                     RSAEEHLRVTTESLAGALRSNTVRGAWGEAQLRRLVEVAGLTQYVDFELQQSITSDAG
                     GGRPDMVIRLPGDKSIAVDSKAPMAAYLEASEIPFTATGEEGARRKRLLDDHVKAVRG
                     HIDALGRKRYWEGLQPSPEFVVAFIPSESLLSAALEADPALLDYSFEKHVALASPVNL
                     WAVLKTVAYTWQEQAVTGEARALFQLGTQLYDRLATLAGHADRLRKSLQSTVEHYNKF
                     ANSLESRVLVTARRFPGIDETKLLPQAAVIEESPRMLTAPELDPGSAAERERPALPAP
                     VIPAE"
     gene            14859..>15678
                     /locus_tag="VFQ96_04575"
     CDS             14859..>15678
                     /locus_tag="VFQ96_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU0257096.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTKNTVLAANYPFATIEPNVGVV
                     SLPDPRLAVLADLFHSQRVVPAPVSFVDIAGIVKGASEGEGLGNKFLATIREADAIAE
                     VVRGFADDEVVHVAGKVDPAADLETINTELILADLQTLASAVPRYEKEVKGKRTDPAV
                     LQAATEAVAWLDVGKPLSASKLDLAPIRELGLLSAKPFIYVFNVDEDVLGDELRKAEL
                     ERLVAPAKAVFLDAKLEAELIDLTPEDAAELLASTGQTESGLDQLARIGFATLGL"
BASE COUNT         2198 a         5374 c         5487 g         2619 t
ORIGIN      
        1 ccctgccgcg gctcagcgac gcggcggagc tcgtgctcta tcgcgtcgcc caagagagtc
       61 tgaccaacgt cgccagacac gcgcacgcca cccgggccgc ggtgtccctc accgcctccg
      121 ccgacgcggt gaccttgcgc gtcaccgacg acggcccggc ggggagcttt cactttcagg
      181 agggcgcagg aatcaggggt atgcgtgagc gggcgttgct cgtgagggcc cgcctcgaca
      241 tcggcgctgc gccggagggc ggaaccgagg tgctcctgag ggtgccgacc gcgacgcgag
      301 ggagctgagc cgatgcccgc cacgtccacg acgcccatcc ggatcctgct cgccgacgat
      361 catgcgctcg tgcgcaaggg gttgcgcctg atcctcgact cggagccgga cctccgcgtc
      421 gtgcgtgccg ttgacgacgg tgcggccgcc gtggaggccg tggccgccgg cgacatagac
      481 ctggccatcc tcgacatcgc gatgccccgc atgaccggca tccaggcggc ccgcgagatc
      541 tctgcccgca cgccgcagac caggatcctg atcttgtcga tgtacgacaa cgaacagtac
      601 tttttcgagg ccctgaaggc aggtgcctcc gggtacgtgg tcaagtcggc cgcagaccgg
      661 gatcttatcg aggcctgccg cgcgatgatg cgcggcgaac ccttcgtcta tccgggcgcg
      721 atcgccgcac tcatccgcga ctaccttcgc cgtgaccggc agggggaacg ggcgtcgtcg
      781 acgctcctca cgccgcgcga agagcagatc accaagctca tcgccgaggg cgagtcgacc
      841 aaacagatcg cggccgctct gtccgtcagt gtgaagaccg tcgaacggca ccgggcaaac
      901 atcttgcaga agctcggaat gcgtgaccgg ctcgacctga cccggttcgc catccgcacc
      961 ggcctcatcg agccctgagc gccgcgcgcc ttgggccggc ctgccgacag aaggcgcgcg
     1021 gacggctcag gcgaacgtgt cgaccttctc gatgcgcacc gcgatgttct tgccgttcgg
     1081 cgcctgatag cttgtcgtgt cgccgacctt cagccccagg atggcgctgc cgagcgggct
     1141 ctgctcgctg tagaccgaga tctcggtgtc gcccgcgatc tcgcggctgc cgagcacgaa
     1201 cacggtctcg tcgccggcga tcgtcgccgt gatgaggagg ccgggttcca cgattccgcg
     1261 gttctccggg gcgtcgccga cactcgccgt gcgcagcaac tgggtgaggg tcgcgatgcg
     1321 ggcctcgatc ttgccttgct cgtctttcgc cgcatggtag ccgccgttct ctcgaaggtc
     1381 cccctccgcg cgggcctcct cgatcttctt cgcgatctca acccggccgg tcgtcgtgag
     1441 ctcctcgagc tcggcggcga gtcgatcgta cgcgtcacgg gtcagaaaac tgaccgacgt
     1501 ctcctgagac atgctgcgct ccttcgtgcc agacagcggc actcgtcgac ataaagtaga
     1561 cgccccgaca tgactgccgg ggcgtgcgta gctgccagct taacggaccc agcagtcgga
     1621 aatcaatcct gcgactgcgg gctgtacggt gcggatcgtc tgttcgtgcg cggtctgctg
     1681 tgcgctcgtt gcggggaagc tcaccacttt ccacccgacg atcgcgtggt cttcgctgtc
     1741 ggcctctacc gcgcagctat acgccgttcc gggggtgacg ctgaccgtgt atcgcaccgt
     1801 cacgctcatc ccgtgatgcg tgatcgaggt gccagtcacg ttggagccga gtgtgctgga
     1861 cgtgctgccg atgccgttca cggcgatcgc ccagacgaag aagatgatga cggccaaggc
     1921 ggcgaaaccg ccgatcagcc acccctccga gatgcggtgc ttgcggacgc ggccgtagcg
     1981 ggtctggacc tcgctcgcct ccgactcctg cacaggcatt gtctcccact ctccagcgtc
     2041 ttggccccgc ctcgcctaag cttgaggcaa gactacgtgt cgcgcctgtg cgtcgccggc
     2101 ccggtgccgg ccggcgggta ggcgcggcag tgatggagaa ttgtcgtgac ccaccgattg
     2161 atggcggtgc atgcccaccc ggacgacgag tccagcaagg gcgcggcgac ctacgcccac
     2221 tatgcggcgc agggcgacga ggtcatggtc gtcacgtgca ccggcggcga gcgcggcgac
     2281 attctcaacg agaacctctc ccaactggcc ttggcacgcc gcgacttgcc ggggctgcgc
     2341 cgttccgaga tggccgccgc gcaggcggtc atgggcgttc agcacaggtg gctcggttac
     2401 gtggattccg gcatggcacg cgaagacggc acggtgccga cgggctcgtt cgccgacatc
     2461 cctgtcgaga tcgaggcgga gccgctcgtg cggctgatcc gctcgtttca gccgcaggtg
     2521 ttgatcacct atgacgagaa cggcggctac gcccatcccg accacatccg cagccacgtg
     2581 gtggcgatgg aggcctatcg ggccgcagcc gaccccgccc gctatccgtc ggccggcgag
     2641 ccgtggcaga tcgccaagtt gtactacgag cgcatcttga atgcgccgcg gatccgcaag
     2701 gtctacgagc tgttgaagag gcgccagccc gactcgcctt tgatcgccaa gttcgagacg
     2761 atgcgcagct ggctcgacga gcggccgctg caggccacga cgcaggtcgc cgtcggcgac
     2821 gagctcgacg tgcgcgacgc ggcgttgcgc gcgcacgcca gccaggtcgc ccccgacagc
     2881 ccgttcttct tctggccgaa cgatgtcgtc cgtgaggcgt ggccgttcga ggattaccag
     2941 ttggtcgagt ctcagacgag cacaccgctg cctgagaccg acctgtttgc cggggtggag
     3001 gcctgagcat gttcacagct ctgacggcgg gggtgcagat cctcgccgac tcgtcgacac
     3061 cgggcgacga caacgcccag tactatgcgc actcgcccgg ggcctgggcg ttcttcacgc
     3121 tgctcggcat cggcatcgtc gtcgtgctct tgtgcgccga catggtgcgg cgcatgcgac
     3181 gggtgcgata ccgcgccgag atcgccgcga agctcgacgc agaagaggcc gagctcctgg
     3241 cgcggcctca caccgccgaa gggggagacg gtcgtggccc gtcgggcagg gtctcgccgg
     3301 ccggcccggc gacgccgtga gggtgtggcg ctaacgtagc tacgtgacgc cacgctcctc
     3361 cggccgcggg cccctctacc gcatctatca gaggcggctg cgtcgctcgt tcgaccccgg
     3421 ggtgctaccc cggcacgtcg cgatgatcgt cgacggcaac cggcggtggg cacggctggc
     3481 cgggctcgac agcgtggccc ccgggcatcg tgccggcgcc gcgaagatga aggagttcct
     3541 tgcctggtgc gaccggctcg gcatcggcgt cgtcacgctg tacctgctct cgaccgacaa
     3601 tctggcgaat cgcgacgccg ccgagcttgc cgacctgacg acgatcatcg ccgagcttgc
     3661 cgacgacgtg tcccgggtgc ggggctggcg ggtacagcac gtcggatccg acgcggggct
     3721 gccgccgggt ctcgcggcgg ctctgcgggc ggcggaggag cgctcggcac agggtacggg
     3781 actgcacgtg aacctggccg tcggctatgg cggccgcaaa gagatcaccg aggcggtacg
     3841 ctccatcatt cgagaccacc acgaatccgg cggcagtctc gagacgttgg ccgaatccct
     3901 gacgcccgag atgatcggca ggcatctgta tactcgcggc caggcggacc ccgaccttgt
     3961 catccgaacc tcgggggaac agcggctcag cgacttcatg ttgtggcaga gcgcgcacag
     4021 cgagttctac ttcgtcgagg cgctgggccc cgacctacgc gaggtggatt tcttgcgggc
     4081 catccgcgat tacacgcgtc gcaaacgcag gtacggaggt tgacgtggtg atagacggat
     4141 acctgtcggg cttcaccgaa gagccgggct atctggacta cgcgcgcgtc ggcccgctct
     4201 ctgcaggtgt cgcggcggag gtgctcgcca acacggaagc gttgcgtcgc agccggttcg
     4261 gcagcctcga cgcgatggtc ggggagcacg accggctctg cgcggcagtc gagagggcgg
     4321 tcggcttccc ggcccgcaac gtcgtcttcc aatcgaacac gagcatcgcc ttgatgcagg
     4381 tgcttttcgc gctccgcggc gacgtgcttc tttcgcccga cgagttcccc tcgatgacgt
     4441 tcgcggcggc ccgcgcggcg tcgatgggtg tgtgccggcc gatctggctc gacgccgaac
     4501 ggccggtcac cgcagaggcc gttctcgacg tgctcagcga cgaggtgacg gctttggtcg
     4561 tcagcctcgt cgactaccgc acgggatacc gggtggatct gccggcattg cggcaggtga
     4621 tcggcgggcg cctgctcatc gtcgacgcca cgcagggcct cggcgtggtc gacgccgcct
     4681 tccacgacgc ggacgtggtc gtcagcggcg gtcaagcgtg gctgcgcgcc gggtggggca
     4741 gcggtttcct cgccgtcagc gaccacgccc tcggccttct cgaccctgcc ttggcggggt
     4801 atcccggggc agcggaggat ccattcacga cggtcagcgg gcccgacggc accgtgatgt
     4861 ccttcacggt caacgagccc gactatgcgg ccgcggcccg cctggcggcc gcgctggagg
     4921 aggtcgccgg cgcgggcgcc gcacagatcg agtcgctcgt gcaggaaaag gtggaggagc
     4981 tcatcgccgc cgccgacacc gccggcgtgc cggtcgcgtc gccccgcgat gccgcccgcc
     5041 gagcgggcat cgtcacgctg cgccccgagc ctgccgccgt gacggccttg gcggcggcct
     5101 tcttcaaccg tggcgtcacc gtcaccacgc aggcgggcac cgtgcgcctg agcgtccacg
     5161 ccggcacctc cgacgcgacg ctcggcatgg tgcgtgacgc cctggcggtc ttcgccgcct
     5221 gagcccgtct cgccgcttcg ctcccaaccc ccgtcttcgt tgttctgtgg atgctccccg
     5281 acgtgccgag gcgacggcgg ctacggtcgc ctcgtggctc gcatccctga cacccgaggt
     5341 cttgctcccg actctctcga gggctggctg ctgccgtgtc gggccggtct tttgccgggt
     5401 caggcgtctg ccggcgccca ggggggccgc gcgaggcggc gtctggctgc cggtctcgcc
     5461 gtcgcgcttg cggcggcggc cttgtccgcc tgggcgcttt gttgggcggg cgtcggcccg
     5521 agggccttgc tgccgctcta tctggcgggt gtcagcctgc cgctgtgcgc gtgggatctc
     5581 gcgcaccgcc gccttccgaa cgccctcgtg ctgcccggct acgccgtcgc ggccgtgggg
     5641 atcctcgggc tccgtctcgc gggcgagccg gccgccccgg cgctcgccgc gggcgccgga
     5701 acgttcggtt tcttccttct tctggccctc gccggcggca tgggcatggg agacgtgaag
     5761 ctcgccggcc tcatcggcct ggcgtcgggc gctctcgccc ccggtgcggc gctcctggcg
     5821 gtcgttatcg cgttcctcac gggcggcgtc gcgggcatcg cggcctggct ggtacccgaa
     5881 ctgcgtaccc gcggcatacc cttcggcccg tacctgctct tggggctgtg ggcggcgccg
     5941 gcgttcgcct gagaagatgt cgggcggcag gcgtcaggac tgcgccggat gcgtcatcga
     6001 gagaacgtcg agcctcgcgt cgagctcctc cagcgtgatc tctccgcgct cgacgaagcc
     6061 gaggtcgatc gtcgcctgac ggatcgtcac agaatcggcg atcgcgtgtt tcgcgatctt
     6121 cgccgccgcc tcgtacccga tcaccttgtt gaggggtgtc acgatcgacg gggacgactc
     6181 cgccaacgcc cgtgcccggg cgacgtcggc gcgcgcgccc gcgatcgtct tgtcggccag
     6241 gagccttacg gcgttggtga gaaggcggat cgattcgaga agcgccgtgc ccatgatcgg
     6301 gatcgagacg ttcagctcga acgccccgga ggcgccgctg aaggcgatcg tcgcgtcgtt
     6361 gccgatcacc cgcgaggcga ccatgagtgt cgcctccggg atgaccgggt tcaccttgcc
     6421 gggcatgatc gaagaacccg gctgcaggtc gggcaggtgc aactcgccga gcccggcgtt
     6481 cgggccggag ctcttcaggc gcagatcgtt gcagatcttc gtcaggctga cggcgatgac
     6541 gcgcagcgca ccggaagcct cgacgagcgc gtccctgttc gcctgcgcct cgaagtggtt
     6601 gcgggcctcg ctgatcggca gccccgtctc ctctttcagg agttcgatga ccatcgcggg
     6661 aaaggccgcg ggggtgttga tccccgtgcc gacggccgtg ccgcccaacg ggacctcgct
     6721 cacccgggtc agggaggcgc gcacccgttc cacgccgagt cgcatctggg ccgcgtagcc
     6781 gctgaactcc tggccgaggg tcacgggagt ggcatccatc aggtgggtgc gccccgccgt
     6841 caccacctcg gcccactgcc tcgccttctc ctcgaattcc ttggcgaggt gctccagggc
     6901 ggggatgagg tcggaggtca gggctgccgt gaccgcgacg tgcaccgagg tggggaacac
     6961 gtcattcgac gactgtgacg cgttcacgtg gtcgttgggg tgcacgggcc ggccgaggct
     7021 ctgcgacgcg agggtcgcaa gcacctcgtt catgttcatg ttcgacgagg tgccgctgcc
     7081 cgtctggtag atgtcgacgg ggaactcggc gtcgtgtcgg ccttcggcga cctcgtcggc
     7141 ggcgtgggcg atcgcctccg ccagctgggc gtccagcacc ccgagccgct cgttcgcccg
     7201 ggccgccgac ttcttgatgc gggcgagcgc cgcgatctgc gcccgttcca gccgggtgcc
     7261 cgagatgggg aagttctcga ccgcccgctg cgtctgcgcg ccgtagagcg cggcggccgg
     7321 aaccctgacc tcgcccatcg tgtcgtgctc gatacggaac tcgttggagt cgtccacgtc
     7381 gccgtgatcc tttgctgccg ggcccttctg gggcgctgtg gtgggttgta aggtcagtcg
     7441 agcccgcggg tgcagaccgt ctcgaccggg ccggaaagcc ggtagtaggc gcccacgatg
     7501 gcgagggtgc cggcctccag cgccttgcgg gcgatgtccg actccgtgag gagcaactcg
     7561 atgctcgccc gcacgtgtgc cttgccgacc tcggccgcgt ccaccgaggc cggatcgagc
     7621 gtctcgcctt ccgcggtgcc ggccacagag tgcacggcgg aggcgatcgg cgcgatcgtc
     7681 gcggcgatgt gcggcggcag cgcggccgcg ccggcaccct gcgcgtcgat ggcggcccgt
     7741 accgcaccgc agcgctcgtg cgcggcgatg acaaggatcg gggtcgtcag ggggccgagg
     7801 ccgtattcga tggagccgat cgccgattcg gtgagcacgt gtccggcgtt gcgcacgacg
     7861 aaggcgtcgc ccaggccgag gtcgaagatg tgctccaacg ccacccggga gtccccgcag
     7921 ccgacgagcg tcgcaaacgg gtgctgctcg aaggcgagct ccgcccgacg tgcggcgtct
     7981 tgccgcggat gggttcgctc gtcggcagcg tagcgggcgt ttccgtcgcg caggagttgc
     8041 caggcttggg cgggggtggg caggtcggtc atcgtgtcgg tgcgctcctt agtcgtgtcg
     8101 cggttatttg gccggagatc gagcccgcga cgtgctccac ctccgcgagt gaggcggagc
     8161 cgaagaccgc gtaggcgttc ttgccgtagg tgacggtgag gccgtaccgc acggcgctgc
     8221 cggccgacgt gtggttgtcg aacaggttcc aggtgatccc gtcgatggtg cggtggcctg
     8281 cggccgctgc gggggagagc tggttcgccg cccaggtcgg gttggcgtcg aagccctgct
     8341 cgatcgtgac gaagccgtcc gaaggcgtca cgaaacccag ctgccagtag tcgatgccat
     8401 cgggcgtgtt cgtgttgagg cgggcgacgt tgcaacgcca ggaggaatcc aggtcgggca
     8461 cgacgagcgc cgtcgactcg gcacccgaga cctgcttcgc gatggaacga aagtctgcgc
     8521 gcggcgcggc ggtcatcttc ggggtgggtg tcatcatggc gacgacgacg acggcggcgg
     8581 cgacggcaag gagggagacg atcaggttcc ctatggtgcg gcggctgcgc cgttcacggg
     8641 tggctgcctc gcggcgcaga gccgcctcct ccggagtctc cggcctgcct agttctgcga
     8701 caatcgcggg agccttcggc gtgcggctca tcgtgcagtc tttccgacgg ctccgccgac
     8761 ctcgtgcctg gcggtcattc gacgccctcg ctcgcggccg cgcctgcttc gtgcgtaccg
     8821 cgcaccgcgt cgaggcgctc cctcgcgccg aggagccact tctcgcaccg cgccgccaga
     8881 gcctcgccgc gttcccacag caccaaagat tcctccagcg tcgccgaacc ctgttccagg
     8941 aggccgacga ccttcacgag ttcgtcgcgg gcctcctcgt agctgagttc ctcgaagccg
     9001 ggctccgcgg tatcggtggc catggcaccc atcttaacgt tccgccgccc ggcgcccccg
     9061 tggttgcggc gcccctgtgg aggttgcgcc gattgcaccg ttagcgaacg tgaggcgtag
     9121 ccgggtgccg gccggcgctt cggcggcgga ccgaaccacc gcgccgcctt ctttgtgggc
     9181 gatcgcgtag ccacggtcga gcgtcatctg cggggagagg gcgcgtagct gcgcgcgtag
     9241 cctggccgtc tcggcgctcg cgcgttcgac ggtacgcgag acgaggtcgc gggaacgctc
     9301 catgtaccgg ccgagctcgt cggcacggct gtcgacgatc cagtcgcccg aggcgagcgc
     9361 cggcctcgtg cgcagtcgct ccagcgcctc cgcctcgccg cgtaccaggg cggtcaagcg
     9421 caagccgatg cggcttcggg cctgctgcac ccgcagaatc tcctcggcca cgtcgggcac
     9481 cacgcgtttc gcggcgtctg tgggcgtcga ggcccgcagg tcggcgactt cgtcgaggag
     9541 cgggcggtcc gcctcgtggc cgatggcact gacgaccggg gtggcactga gcgccgcggc
     9601 ccgcaccacc ttctcgtcgc tgaagctcaa gaggttctga aagtctccgc cgccgcgggc
     9661 gacgatgatc acgtcgacct cggggtcggc atccagccgc tggagcgccg ccacgacctc
     9721 gtcgacgctg cggtcgccct gcaccgcggc gtacgcggta cgaaaccgga cggctggcca
     9781 gcgcagcagg gcgttacgct tcacgtcctt ctcggcgtcg gagtcgcgcc cggtgacgag
     9841 cccgatgagg cgcggtagaa agggcagcct cttcttgcgt tcggcgtcga agaggccttc
     9901 ggcggcaagc tgtcggcgca gcctctcaat gcgctccagc aggtcgccga ggccgacgtg
     9961 gcgcatctcc tgcacctgca tcgtcaaggt gccgcccttg acccagtagt tcggcttgac
    10021 ctgggccaca acccgggcac cgcggacgag gtcgtcgggg atccgactgc gggtcctcga
    10081 ccagatcgtg aaggcgaccg tggcgtcgac gtcgacgtct ttcagccggc cgtaaacgtt
    10141 gccggccgac acgttccacc ccgtgatctc gccctcgacc caggcggtgc cgagccgatc
    10201 gatgtagccc cgcagcttct cgcccagcac gtgaaccggc cagggtgcgt cgggtgtgga
    10261 gagcgcgtct gtcatgccga tccctcccgg ggccgggcgt cgtgaacgcg tgcggatgcc
    10321 ccgtctgccc agcaggatac cgtgtgccgc cgactgctac gctcgctagc gaagggccgc
    10381 gcaggtcgcg tgccgcgcgg agagcggagc gggagatggg cgacgagcgc gagcggcccc
    10441 ggtacggcga gtatgccact ccggaggagc agcagcggag cattcaggat cccgaggccc
    10501 ggcggcgtat cgccagggct gcctccggtc aggcggccga cgaggggccg gccgcggagg
    10561 ggcctggcgc cggggcccgg ccgccgaggc cggcgtctga tcacggcctc gagcgagcgc
    10621 ggggctcctt cgcggcgccg acgggctcgc atcgtggcat gccaggagtg gcttctgccg
    10681 acgggagggc gcccggccgg cgctgggatc tcatcctgac gctcgtcttg ctggcggtgg
    10741 gcctgctcga cgtcgtgacc tcggtctcca gctacctgca cctcgatcag accctggccg
    10801 ccgtgtatca acggctcggc gttcacgcga gccctgcggc aggtgacgcg gccaccgcgg
    10861 gcgccgcgct cgtcgccgtc cgggtcggcc tctgggtcgt ggcggcgggc ctggccgcgt
    10921 ggcgcctgac cgccagacgg ctggcgttct ggatccccct cgtcgcggcg gccgtctcgt
    10981 acgttgtgat cgtcgtggtg atgctggagg tcgtctttca agaccccgcg gtgcgcacgt
    11041 tcctgcagaa ccagtgacgg ctcgccggct cagtggccgg agtgccccgc tgaacccagc
    11101 tgctgagcgg cctggatcac ccgctgcgcc atcgccgtct ccgcaacctt gccccaggcg
    11161 cgcgggtcgt attgcttctt cacgccgacc tcgccgtcga ccttcagcac gccggcatag
    11221 tgctcgaaca tgtagccggc gatcgcgcgc gtgaacgcat actgcgtgtc ggtgtcgacg
    11281 ttcatcttca ccacgccgtt ggcgacggcg gtggcgatct cttcggggct cgagccggag
    11341 ccgccgtgga agacgaggtc gaggggcttg tcggccttgc cgtatttctt ggcgatcccg
    11401 gcctggatct cgccgagcag gtcggggcgc agcttcacgt tgccgggctt gtagacgccg
    11461 tgcacgttgc cgaaggtgag cgccgccatg tagcggccgt tctcgccgaa gcccagcgcg
    11521 tcgacagcct tcgacacgtc gccgacggtc gtgtagaggg cttcgttggt gccttcgtgc
    11581 ttgacgccgt cttcctcacc gccgacgacg ccgacctcga tctcgaggat cgtgtggatg
    11641 gcgctggtgc gcgcgatcat ctcttgagcg atgcggatgt tctcgtcgag cggcaccgcc
    11701 gatccgtccc acatatgcga ctggaagatg ggctcgtgcc ccgccttcac ctcctcctca
    11761 ctggccgcca ggagcggcag caagaagctg tcgagcgccg gtttggggca gtggtcggtg
    11821 tgcagggcga cggtgaccgg atagttcttg gcgacctcgg tcgcgaactt cgcgaaggcc
    11881 aaggccccgg cggctcgggc cttgatcgtc tggccggcga agtagtctgc gccgccggtc
    11941 gtcacctgga tgatgccgtc gctgcccgcc tcggtcaggc cctgcagaac cgcgtggatc
    12001 gtctgcgacg acgacacgtt gaacgcgggg taggcgaaac ccttcttttt ggccttgtcg
    12061 agcatctcgg cgtattggtc gggggttgcg acgggcatgg agactcctcc tgggttgtcg
    12121 atctcggcgc aagtctagtc aagcggcccc gacgctccgc gcccgccccg tgctctgctc
    12181 gtctgaagcg gccgagggat cgtgatcgct ccgttatcgg ctgcgggcgg agcctcgcac
    12241 gggcctgagt accctgggta tgcctggcat cgtatgtatg acggttgccg aaggcccgcc
    12301 cgcccgcccg gcggcgggga acacgaagat aggacgaagg cgtgactacc actgacccat
    12361 cgggactgcc ggctgcgacc ggagggcccg cggtacccgc ggtctcgcct ctgaaccccg
    12421 atcggaatct cgggatggag ttggtgcgcg cgaccgaggc ggccgcgatc cgcgcggcgc
    12481 cgtggatcgg caaagggcag aagaataccg cggacggcgc cgccgtcgac gccatgcggg
    12541 cgtttctcgg taccgtcaac ttcgacggcg tgatcgtcat cggcgaggga gagaaggacc
    12601 acgcgccgat gctcttcaac ggcgagcatg tcggcaacgg ccggggcccc gcctgcgaca
    12661 tcgccgtcga ccccatcgac ggcacctcgc tgaccgcgta cggccaccag aacgcggtgt
    12721 cggtgatcgc cgtgtcggat cgcggcagca tgtacgaccc ctcggccgtc ttctacatga
    12781 acaagattgt ctgcggattc gagggcatcg gcgtcgtcga catccgcaag ccgatcggcg
    12841 agaacattcg cgcgctcgcc aaggcgaagg gcaagcacgt ctccgagatg acggtagcga
    12901 ttctcgaccg gccgcgtcac gcgcagctca tcgacgaggt gcgcgccgcc ggcgccggca
    12961 cccggctgct cctcgacgga gatgtcgcgg ggggcatcaa tgcggcgcgt tacgcctcgt
    13021 acatcgacat gtgcgtcggt gtgggcggca cccccgaggg catcatcacg gcctgcgcgg
    13081 tgaaggcgtt gggtgggctc gtgcaaggtc agctcatgcc gaaggacgac gcggaacggc
    13141 agaaggcgat cgacgccggc cacgacctcg accgggttct cgaccaggac gacctggtca
    13201 ccggagacaa cacgtatttc gtcgcgacgg gggtcacgaa cggcgagctc gttcacggcg
    13261 tggaacgcaa gggaccggtg atccgcaccg agtcgatcgt gttgcgctcg aagtcgggga
    13321 cgatccggcg catcgtcgcc gagcacctcg tcgacaagtg gagctgaggc ggccggcggc
    13381 cgtcactcgg cggggatgac gggggccggc agcgccggcc tctctctctc cgccgcggag
    13441 ccggggtcga gttcgggtgc ggtgagcatg cggggggatt cctcgatcac cgccgcctgc
    13501 ggcaagagct tggtctcgtc gatgccgggg aaccgtcgcg ccgtgacgag cacccgagac
    13561 tccagagagt tcgcgaattt gttgtagtgc tcgacggtgc tctggagaga cttgcgcagt
    13621 cggtctgcgt ggccggcgag ggtcgccaac cggtcgtata actgcgtgcc cagctggaag
    13681 agcgcgcggg cctcgccggt gacggcctgc tcttgccagg tgtaggcgac ggtcttcaag
    13741 acggcccaca ggttgacggg cgaggcgagc gccacgtgtt tctcgaagga atagtcgagc
    13801 agggccgggt cggcttcgag ggccgcggaa agcaatgact cactcggaat gaaggcgacc
    13861 acgaattccg ggctcggctg caggccctcc cagtatctct tgcggccgag cgcgtcgatg
    13921 tggccgcgca ccgccttcac gtggtcatcg aggagccttt tgcgccgtgc gccctcctcg
    13981 cccgtggcag tgaacgggat ctcgcttgcc tcgaggtagg ccgccatcgg ggccttcgag
    14041 tcgaccgcga tcgacttgtc gccgggcagg cggatgacca tgtcgggccg cccgccgccc
    14101 gcgtcggagg tgatgctctg ctgcagctcg aagtcgacgt actgggtcag gccggcgacc
    14161 tcgacgagcc gacgcagctg ggcctcgccc catgccccgc gcaccgtgtt ggagcgcagc
    14221 gcccccgcca gtgactcggt cgtcacacgc aggtgctcct cggcgctgcg cgcctgggtg
    14281 agttgctccg acagtgcgcc atactgcgcc tgacgctgcg cctccatctg cgtgaccttg
    14341 ccctgcatga gaccgagcgt ctctcgcacc ggggcgaggg cctgcagcac cttgctttcg
    14401 cgtcgttctt gttcttgccg ggcctcggcc tctgccttgt ggcccgcccg caccttctcc
    14461 agctcgtcgc gcaagagctg cacctgggcg cgcaactctt gggcgctcgt ctcggcggcc
    14521 gcacagcgct ccgcgagctg ggcacggcgt tcggcgtcct cctgctgcag ccgtgcacgc
    14581 tcttgcgcct cttcgtgccg aacgcggtcg atctcgttcg cgtggcgctc cgccacgagt
    14641 tccgggctct cggctgcgcc ggcgccgcgc atggagcgcg tcacggcggc aagcagggcg
    14701 acgaccgcgg ccaagagggc cacgccgatc acgagaagca cgatatccat agggccagtg
    14761 tgccagccgc cgccgacacg gcggaggcct cctgcggagc acaggccgat tcgaccccgc
    14821 cccgagggtg ccacggggcg ccgatagact tgggccccgt ggcactcacc atcggaatcg
    14881 tcgggctgcc gaatgtcggc aaatccaccc tcttcaacgc gttgacgaag aacacggtgc
    14941 tggcggcgaa ctacccgttc gccacgatcg agccgaacgt gggcgtcgtg agccttcccg
    15001 acccccggct ggcggttctg gccgacctct tccacagcca gcgggtcgtt cccgccccgg
    15061 tctcgttcgt cgacattgcg ggcatcgtca agggtgcgag cgagggggag gggctcggca
    15121 acaagttcct cgccacgatc cgggaggccg acgcgatcgc ggaggtcgtg cgcggcttcg
    15181 ccgatgacga ggtcgtgcat gtggcgggaa aggtagaccc tgccgccgat ctggagacga
    15241 tcaacaccga gctgatcctc gccgacctgc agaccctcgc cagcgccgtg ccgcgctacg
    15301 agaaggaggt gaagggcaag cgcaccgacc ccgccgtgct gcaggcggcg acggaggcag
    15361 tcgcatggct cgacgtcggc aaaccgctct ccgcctcgaa gcttgacctg gcgcccatcc
    15421 gcgagctcgg cctgctgagc gcgaagccct tcatctatgt cttcaacgtc gacgaagacg
    15481 tgctgggcga cgagcttcgc aaggcggagc tggagcggct cgtcgcgccc gcgaaggccg
    15541 tcttcctcga cgcgaagctg gaggccgagc tcatcgacct gacccccgaa gacgccgccg
    15601 agctgctcgc ctccaccggg cagaccgaga gcggtctcga ccagttggct cgcatcggtt
    15661 tcgcgacgct cggcctgc
//
LOCUS       DASTNL010000145         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_101006, whole genome shotgun sequence.
ACCESSION   DASTNL010000145 DASTNL010000000
VERSION     DASTNL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101006"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFQ96_04580"
     CDS             <1..314
                     /locus_tag="VFQ96_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0257097.1"
                     /translation="IEEQVASIWAGSNGKMDDLPVEEVLRFEAEMLEHLRRNTTLLTT
                     IRDSGRLEPDVRDQLGEAIDAFKKEFVAEGAPAGVGREEFDAIDAGDVNQEQIVKGRR
                     A"
     gene            377..1273
                     /locus_tag="VFQ96_04585"
     CDS             377..1273
                     /locus_tag="VFQ96_04585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU0257098.1"
                     /translation="MGAQLRVYRQKIRSNQATKKITRAMELISASRIQKAQARVAAST
                     PYAREITRAVSAVATYSNVSHILTTEPETVARAAIVIFGSDRGLAGAFNANVLKESEE
                     LAELLRGQGKEVVFFLVGRKPAGYFAFRQRPSERTWTGGTDNPQFETAREIGQALLAS
                     FLLDASDGGVDEIHIVYNRFVSMLTQTPQVVRLLPLEVVEGIEAPTADTQLPLYEFEP
                     DAETVLDALLPVYIESRIFNAMLQSAASKHAATQRAMKSASDNADKLITTYTRLANNA
                     RQAEITQQISEIVGGADALAQA"
     gene            1307..2773
                     /gene="atpD"
                     /locus_tag="VFQ96_04590"
     CDS             1307..2773
                     /gene="atpD"
                     /locus_tag="VFQ96_04590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU0257099.1"
                     /translation="MTPTATAAGQETTSAVTGVGRVARVTGPVVDIEFPHDSIPGIYN
                     ALKTTVALGGESQEITLEVAQHLGDDLVRAVALKPTDGLVRGQEVRDTGAPISVPVGD
                     VTKGHVFNVIGEVLNAQPGETIEVTERWPIHRKPPAFDQLESKTQLFETGIKVIDLLT
                     PYVEGGKIGLFGGAGVGKTVLIQEMIQRVAQDHGGVSVFAGVGERTREGNDLIHEMEE
                     AGVFDKTALVFGQMDEPPGTRLRVALSGLTMAEYFRDVQKQDVLLFIDNIFRFTQAGS
                     EVSTLLGRMPSAVGYQPNLADEMGILQERITSTRGHSITSLQAIYVPADDYTDPAPAT
                     TFAHLDATTELTRELAAQGLYPAVDPLTSTSRIMDPRYLGEDHYRVATTVKQILQKNK
                     ELQEIIAILGIDELSEEDKITVERARRIQQFLSQNTYMAKKFTGVEGSTVPLKDTVES
                     FDAICRGDFDHVAVQAFFNVGAISDVEEKWAEIQKENG"
     gene            2776..3045
                     /locus_tag="VFQ96_04595"
     CDS             2776..3045
                     /locus_tag="VFQ96_04595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU0257100.1"
                     /translation="MAATLKVNVVSADREVWSGEATMVVAKTVEGEIGIMAGHEPILA
                     ILAAGEVRLHVPDGPTLTAKADDGFLSCANNTVTIVAGEAELAAA"
     gene            3050..>3328
                     /locus_tag="VFQ96_04600"
     CDS             3050..>3328
                     /locus_tag="VFQ96_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU0257101.1"
                     /translation="MLVLLPPAETKRAGGTGPALRLDRLAWPEALGAARRQALDALVA
                     LANDEEASRRALHLGPRGAGEVRKNRDLLTAATMPALQRYDGVLYRAAG"
BASE COUNT          619 a         1102 c         1127 g          480 t
ORIGIN      
        1 cgatcgagga gcaggtcgct tccatctggg cgggcagcaa cggcaagatg gacgacctgc
       61 ccgtcgagga ggtgctgcgt ttcgaggcgg agatgctcga gcacctccgc cgcaacacga
      121 ccctccttac gaccattcgc gacagcggcc ggctggagcc cgacgtgcgc gaccagttag
      181 gcgaggcgat cgacgcgttc aagaaagagt tcgtggccga gggcgcaccc gcgggcgtgg
      241 gccgcgaaga gttcgatgcc atcgacgccg gcgacgtcaa tcaggagcag atcgtcaagg
      301 gccgccgggc gtgacggcgc gaggcgcctg gagcgacgtc gggcgtcggg gcggcaacgt
      361 gaagaaggag gggtaaatgg gagcgcagct gcgggtctac cggcagaaga tccggtcgaa
      421 ccaggcgacg aagaagatca cgcgcgccat ggagctcatc tcggcctcac gcatccagaa
      481 ggcgcaggcg cgggtcgccg catcgacccc gtatgcccgg gagatcacgc gggcggtctc
      541 ggccgtcgcc acgtattcga acgtgtcgca tatcttgacg acggaaccgg agacggtggc
      601 gcgcgccgcg attgtcatct tcggctccga tcgcggcctc gccggcgcgt tcaacgcgaa
      661 cgtcctcaaa gagtcagaag agctcgccga gcttttgcgt gggcagggca aagaggtcgt
      721 gttcttcctc gtcgggcgca agcccgctgg atacttcgcc ttccggcagc ggccgagcga
      781 gcgcacctgg acgggcggca ccgataatcc gcagttcgag accgcacggg agatcggtca
      841 ggcgctgctc gcctcgttcc ttctcgacgc ctccgatggc ggcgtcgacg agatccacat
      901 cgtctacaac cgtttcgtca gcatgctcac gcagacgccg caggtcgtgc gcctgctccc
      961 gctcgaggtc gtggagggca tcgaggcccc gacggcagac acgcaactgc cgctctacga
     1021 gttcgagcct gacgcggaga ccgtgctcga cgcgctcctg ccggtctaca tcgaaagtcg
     1081 catcttcaac gccatgctgc agtccgccgc gtcgaagcac gcggccaccc aacgggccat
     1141 gaagtcggcc agcgacaacg cagacaagct gatcaccaca tacacccggc tggcgaacaa
     1201 cgcacggcag gccgagatca cccagcagat ctccgagatc gtcggcggcg ccgacgccct
     1261 cgcccaggcg tgacgaccgc ccgcgaaaga gagaagagat agagcaatga caccgactgc
     1321 aaccgctgcc ggccaggaga cgacatccgc cgtcaccggc gtcggccgtg tcgctcgtgt
     1381 gaccgggccg gtcgtggaca tcgagttccc ccacgactcg atccccggca tctacaacgc
     1441 cttgaagacg acggtcgccc tgggcggcga gtcgcaagag atcacgctcg aggtcgcgca
     1501 gcacctcggt gacgacctgg tgcgtgccgt cgcattgaag ccgaccgacg ggcttgtgcg
     1561 tggccaggag gtgcgcgaca cgggtgcgcc gatctccgtg ccggtgggcg acgtcaccaa
     1621 gggtcacgtg ttcaacgtga tcggcgaggt gctgaatgct cagccgggcg agacgatcga
     1681 ggtcaccgag cgttggccga tccaccgcaa gccgccggcc ttcgaccagc tggaatcgaa
     1741 aacccagttg ttcgaaaccg gcatcaaggt catcgacctc ttgacgccct atgtggaggg
     1801 cggcaagatc ggcctcttcg gcggcgccgg cgtcggcaag acggtgctca ttcaagagat
     1861 gatccagcgc gtcgcgcaag accatggtgg cgtctcggtg ttcgccggcg tcggcgaacg
     1921 cacccgtgag ggcaacgacc tcattcacga gatggaggag gcgggcgtct tcgacaagac
     1981 cgcgctcgtc ttcggccaga tggacgagcc gccggggacc cgcctccggg tggcgctgtc
     2041 cggcctcact atggcggagt acttccgcga cgtgcagaag caagacgtgc tgctcttcat
     2101 cgacaacatc ttccgcttca cgcaggccgg ctccgaggtc tccaccctcc tcgggcgcat
     2161 gccgtcggcc gtcggctacc agccgaacct ggccgacgag atggggatcc tgcaagagcg
     2221 catcacctcg acgaggggcc actccatcac gtcgctgcag gcgatctacg tgcccgccga
     2281 cgactacacc gacccggcac cggccacgac gttcgcgcac ctcgacgcga cgaccgagct
     2341 cacccgagag ctggcggcgc aaggcctcta cccggccgtc gacccgctca cctccacgag
     2401 ccgcatcatg gatccccgct acctcggcga ggaccactac cgggtcgcca cgacggtcaa
     2461 gcagatcttg cagaagaaca aagagctgca ggagatcatc gcgatcctgg gcatcgacga
     2521 gctttccgaa gaagacaaga tcaccgtgga gcgcgccagg cgcatccagc agttcctgtc
     2581 gcagaacacc tacatggcga agaagttcac cggggtggag ggctcgacgg tgccgttgaa
     2641 ggacacggtg gagtcgttcg acgcgatctg ccgcggagac ttcgaccacg tcgcggtgca
     2701 ggccttcttc aacgtcggcg cgatctccga cgtcgaagag aagtgggcag agattcagaa
     2761 ggagaacggc tgagaatggc cgcgaccttg aaagtcaacg tcgtctccgc cgaccgggag
     2821 gtctggtcgg gggaggcgac gatggtcgtc gcgaagaccg tcgaggggga gatcggcatc
     2881 atggcgggcc acgagccgat cctcgcgatt ctcgcggccg gcgaggtgcg gctgcatgtg
     2941 cccgacggtc cgacgctgac ggcgaaggcc gacgacggct ttctctcgtg cgccaacaac
     3001 acggtgacga tcgtcgcggg cgaggcggaa ctggcggccg cgtagccgca tgctggtgct
     3061 tctgccgccg gccgagacga agagagcggg cggcacgggc ccggcgttgc gcctcgatcg
     3121 gctggcgtgg ccggaggccc tcggcgccgc ccgtcggcaa gctctggacg ccctcgtggc
     3181 cctggcgaac gacgaggagg cctcacggcg cgccttgcac ctcgggccgc gcggcgcggg
     3241 agaggtacgg aagaaccgcg acctgctcac cgcggccacc atgccggccc tccagaggta
     3301 cgacggcgtg ctctaccgtg cggcgggc
//
LOCUS       DASTNL010000146         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_471925, whole genome shotgun sequence.
ACCESSION   DASTNL010000146 DASTNL010000000
VERSION     DASTNL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471925"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFQ96_04605"
     CDS             complement(<1..1303)
                     /locus_tag="VFQ96_04605"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU0257102.1"
                     /translation="MTDDAPTPDLSTTAGKLADFRLRNREAVATGGEAAAEKQHAKGK
                     LTARERIDLLLDPGSFVEQDEFVRHRTHAFGMQKKRPYADSVVTGTGTIHGRQVAVYA
                     QDFTTFGGSLGEVAGNKIVKVMDTALRLGIPIVGLLDSGGARIQEGVVALGKYGEIFR
                     RNTQASGVIPQISVIMGPAAGGAVYSPALTDFVIMVDKTSQMFVTGPDVIKAVTGEDV
                     GMEELGGALTHNKVSGVAHYLATDERDALDYVRTLLAFLPDNNLSEAPEYQTQAPLEI
                     TDADRALNTIIPDSPGQPYDMHEIIDILVGFGRVEGRPVGVVANQPMQFAGTLDIDAS
                     EKAARFVRTCDSFNIPVLTFVDVPGFLPGTDQEWNGIIRRGAKLIYAYAEATVPLVTV
                     ITRKAYGGAYDVMGSKHLRADLNLAWPTAEIAVMGAAGAVNI"
     gene            1554..2411
                     /locus_tag="VFQ96_04610"
     CDS             1554..2411
                     /locus_tag="VFQ96_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0257103.1"
                     /translation="MDLPWAARHAARLCVVAESPSTNTELIGRVRHDPASTWPSLSVL
                     ATDRQSAGHGRHGRAWVAPAGCCLAASVLVRPRGRAAEPESLGWLPLVAGLAMVRAIE
                     QLGVPVGLKWPNDVLAGPAAARPGKLVGVLAELAVTPDGPAVVLGTGVNLTLAEAQLP
                     VPTATSLALCGVAHPDPDAVLAPYLAALARLIGRLEEAGPLGGAVVAGLSHESGGPAA
                     RGGPEAGSIRSQVREACTTLGRPVRVEAPGGLLTGTAVDIDEGGRLVVENAAGRTAVS
                     AGDVTHLRY"
     gene            2521..3063
                     /locus_tag="VFQ96_04615"
     CDS             2521..3063
                     /locus_tag="VFQ96_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU0257104.1"
                     /translation="MADVHDTAPIEHIIVRLRPRGTFLAVSALLVIADAAAVGYLAGR
                     MGVWWHEVVLWLAGAALVVLFALAPLLAWLSRRYTVTSRRVIVQRGLVVRVRQELLHT
                     RGYDVTLSRSWFQALTGCGDVGINSGLSQPVWLRGVPDARRVQMLLQDLAESNRSFVA
                     DRRRAADAGGPDVTTAWGVR"
     gene            3083..>3986
                     /locus_tag="VFQ96_04620"
     CDS             3083..>3986
                     /locus_tag="VFQ96_04620"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU0257105.1"
                     /translation="MDTVGVIGGGQLARMMIPAAIALGVRIRVLAETEDMSASLAATM
                     VGDYTDLDTVRAFAAGVDVVTFDHEHVPQRVLQALVADGVRVEPGPEALVYAQDKAAM
                     RRRLGEAGLPMPDWAPVTSLSDLDRFLAAHGGTAVLKTARGGYDGHGVRVVTAGADAN
                     EWLAALAADDSAGSLLVEEKVDFRREPSQQVARRPSGEVAVYPVVETIQQDGVCAQVI
                     APAPETSDAVARECARIGRGVAEQLGVTGMLAVELFETTDGRVLVNELAMRPHTSGHW
                     SIDGAVTGQFEQHLRAVLDLPLGDP"
BASE COUNT          519 a         1338 c         1515 g          614 t
ORIGIN      
        1 agatgttgac ggctcccgcg gcgcccatga cggcgatctc ggcggttggc caggcgaggt
       61 tgagatcggc tcgcagatgc tttgacccca tgacgtcgta ggcgccgccg tacgccttgc
      121 gcgtgatgac ggtgacgagc ggcacggtcg cctcggcgta ggcgtagatc agcttggcgc
      181 cgcggcggat gatgccgttc cactcctggt ccgtgccggg gaggaagccg ggaacgtcga
      241 cgaaggtgag gaccgggatg ttgaaactgt cgcaggtgcg cacgaaccgg gccgccttct
      301 ccgaggcgtc gatgtcgagc gtgcccgcga actgcatcgg ctggttggcc acgacgccga
      361 ccggacggcc ttcgacgcgg ccgaacccga cgaggatgtc gatgatctcg tgcatgtcgt
      421 acggctgccc cggcgagtcg gggatgatcg tgttcagggc gcggtcggcg tcggtgatct
      481 cgagcggcgc ctgggtttgg tattcgggcg cctcggagag gttgttgtca ggcaggaagg
      541 cgagaagggt gcgcacgtag tcgagcgcgt cgcgctcgtc tgtcgccagg taatgcgcca
      601 cgccggagac cttgttgtgg gtgagggcgc cgccgagctc ctccatgccg acgtcttcgc
      661 cggtcacggc cttgatgacg tcggggccgg tgacgaacat ctgactcgtc ttgtcgacca
      721 tgatcacgaa gtcggtgagc gcgggcgaat agacggcccc gccggccgcg gggcccatga
      781 tgacggagat ctgcgggatg accccggagg cctgcgtgtt gcggcggaag atctcgccgt
      841 acttgccgag cgccacgacg ccttcttgga tgcgcgcgcc gccggagtcg agcaggccga
      901 cgatggggat gccgagccgg agcgccgtgt ccatgacttt cacgatcttg ttacccgcca
      961 cctcgcccag cgatccgccg aacgtcgtga agtcttgcgc gtaaaccgcc acctggcggc
     1021 cgtgtatcgt gcccgtgccg gtgacgacgg aatcggcgta ggggcgcttc ttctgcatgc
     1081 cgaacgcgtg ggtgcggtgc ctgacgaatt cgtcttgctc gacgaaactg cccgggtcga
     1141 gcagcaggtc gatgcgttcg cgcgccgtca acttgccctt ggcgtgctgc ttctcggccg
     1201 ccgcctcgcc gccggtggcg accgcctcac gattgcgcag acggaagtcg gcgagcttgc
     1261 ctgccgtcgt cgacaggtcg ggggtgggtg cgtcgtccgt cacgctgccc actctaccgg
     1321 gagcctccgc gcgcccctga gcttccgcac gcttcggtcg cggcgcccga aagacccctg
     1381 cccgctctcg gagtcgcggc gcaccccgga acgatggtgg cccggcagat gcccgctcgg
     1441 gcgccggccg gcgcctcgga gtcgcggcgt gccccgggag caatcggtgg cggtggcggt
     1501 gcgggggcgg aaagtaggga gaggagagag aaggaagaga gaagactggg gggatggatc
     1561 ttccctgggc cgcacggcac gccgcacgcc tgtgtgtggt ggccgagagc ccgtcgacga
     1621 acacggaact gatcggccgg gtgcgccatg acccggcctc cacgtggccg agcctctccg
     1681 tgctggccac ggatcgccag agcgccggcc acggccgcca cgggcgcgcg tgggtggcgc
     1741 cggccggctg ctgcctcgcg gcttccgtgc tggtgcgccc gcgtggccgc gcggcggaac
     1801 cggagtcgtt gggctggctg ccgctcgtcg cgggcctcgc gatggtgcgt gcgatcgagc
     1861 agctcggcgt gccggtcggc ctgaaatggc ccaacgacgt gctggccggc ccggccgcgg
     1921 cccgtcccgg caagctcgtc ggcgtcttgg cggagctcgc cgtgactccc gacggcccgg
     1981 ccgtcgtcct cggcaccggc gtgaacctga ccctcgccga ggctcagctg ccggtgccca
     2041 cggcgacctc gttggcgctg tgcggcgtcg cgcatcccga tccagacgcc gtgctggcgc
     2101 cgtacctcgc cgccctggcg aggctcatcg gccgcttgga ggaggccggg ccgctgggcg
     2161 gggcggtcgt cgccggcctg tcgcacgagt caggtggacc ggccgcgcgt ggcggacccg
     2221 aggccggcag catccgctcc caggtgcgcg aggcatgcac gactttgggc aggccggtgc
     2281 gggtcgaggc tccgggcggc ctcctcaccg gcacggccgt cgacatcgac gaaggcggtc
     2341 gcctcgtcgt ggagaatgcc gcgggccgca cggcggtctc ggccggcgac gtgacgcatc
     2401 tacggtatta ataggggagt gccaccgagt gttccggcgg tggcggccgg tcgtggtcca
     2461 cgacggagcc ggcgcgacga cggggcctcg ggggcaccgg acgggggagg aggcggcggc
     2521 gtggccgacg tacacgacac cgctccgatc gaacacatca tcgttcgcct ccgcccgcgg
     2581 ggcacctttc tcgcggtctc tgctcttctc gtcatcgccg acgccgcggc ggtcggctac
     2641 ctggcggggc ggatgggggt ctggtggcac gaggtcgtgc tctggctcgc gggggcggcg
     2701 ctcgtcgtgc tcttcgccct cgcgccgctg ctggcctggt tgagccgacg ctacacggtc
     2761 acgtcgcgcc gggtcatcgt gcaacgcggc ctcgtcgtgc gggtgcgcca ggagctgctg
     2821 cacacccgcg gctacgacgt gacgctcagc cgcagctggt tccaggcgct gacgggctgc
     2881 ggcgacgtgg gcatcaactc gggcctcagc cagccggtct ggctgcgcgg cgtgcccgac
     2941 gcacgccgag tgcagatgct gctccaagat cttgccgagt cgaaccgttc cttcgtcgcc
     3001 gaccggcggc gggcggccga cgccggcggc cccgacgtga cgaccgcgtg gggcgtgcgg
     3061 taggcggggg agaggcgggc acatggatac ggtgggcgtg atcgggggcg gccagctggc
     3121 gcgcatgatg atcccggcgg ctatcgccct gggggtgcgc atccgcgtgc tggccgagac
     3181 cgaagacatg tcggcctccc tcgccgcgac catggtcggc gactacacgg acctcgacac
     3241 cgtgcgcgcc ttcgcggcgg gcgtcgacgt cgtgaccttc gatcacgaac acgtgccgca
     3301 gcgggtgctc caggcgcttg tcgccgacgg ggtgcgggtc gagcccggcc cggaggcgct
     3361 cgtctacgcg caagacaagg ccgcgatgcg ccgccgcctg ggcgaggccg ggctcccgat
     3421 gcccgactgg gctcccgtga cgagcctgtc ggatctcgac cggttcctcg cggcgcacgg
     3481 cggcaccgcg gtcttgaaga cggcgcgcgg cgggtacgac gggcacggcg tgcgcgtcgt
     3541 gaccgcgggc gccgacgcga acgagtggct ggcggcgctc gccgccgacg actcggcggg
     3601 ttccctcctc gtggaagaga aggtcgactt tcgccgggag ccctcgcagc aggtggcacg
     3661 ccgcccctcc ggcgaggtag ccgtctaccc ggtcgtcgag acgattcagc aagacggtgt
     3721 ctgtgctcag gtgatcgccc cggcacccga gacgagcgac gcggtcgccc gggaatgcgc
     3781 gcgcatcggc cggggcgtcg ccgagcagct cggcgtgacc ggcatgctcg ccgtggaact
     3841 cttcgagacg accgacggcc gagtgctcgt caacgagttg gcgatgcggc cgcataccag
     3901 cgggcattgg agcatcgacg gggcggtgac cggtcagttc gagcagcatc tgcgggccgt
     3961 gctcgatctg ccgctgggag acccgg
//
LOCUS       DASTNL010000147         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_656934, whole genome shotgun sequence.
ACCESSION   DASTNL010000147 DASTNL010000000
VERSION     DASTNL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656934"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /gene="dprA"
                     /locus_tag="VFQ96_04625"
     CDS             complement(<1..1033)
                     /gene="dprA"
                     /locus_tag="VFQ96_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU0257106.1"
                     /translation="MILALDEAAVRLAVDAVANEATAGPAEAGERFARGVFTMIAEPG
                     DAAAGLLLRALGPLRALSHVVRRDDARTVSRLVDDPEDAASLAHTLPAALERWRPRLS
                     SKAAIDAFRHAAHLGASFLVPGDAGWPAGLDDLGDHAPAGLWVRGDATRLVGLSHAVA
                     LVGARAATGYGTAVAAELASGAADRGLAVVSGAAYGIDGMAHRATLASAGLTVAILAG
                     GIDKLYPSGHAELLAGIIRDGVVVAEMPCGFSPTRWRFLLRNRLIAACSTATVVVEAG
                     ARSGSLNTAGHAAELGRALGAVPGPVTSPASAGCHRLIREYDAQLVTCPDDMAELAGL
                     APAEAVLPAV"
     gene            complement(1030..2568)
                     /locus_tag="VFQ96_04630"
     CDS             complement(1030..2568)
                     /locus_tag="VFQ96_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU0257107.1"
                     /translation="MSVARTWAVTLNGLRGTLVEVEADITAGLPFFTIIGLPDAALAE
                     ARDRVRAAAVNSGCALTTHKLTVNLSPASVPKHGAGFDLAVALAALAAGGAVRPDAVA
                     GVVHIGELGLDGRLRPTAGVLPAVLAARRSGFHTVMVPTANAGEAALVPGIEIVAVPS
                     LREAAIRHGAELEPVPVEPLSAPVTPVPTDTAGGGDLAEIIGNPDAVRALQIAAAGGH
                     HLFLLGPPGAGKTMLASRLPGLLPDLDDAEALEVTSVASILGEGVGGRLARRPPFQAP
                     HHATTVAAMVGGGSGGRIRPGAATRAAHGVLFLDEAPEFPRAVLDSLRECLESGRIAV
                     HRADAVAYFPGRFQLVLAANPCPCGNYGTGRECSCAPNARRRYLARLSGPLLDRVDMR
                     LCLDRVTVAQLRLGAGDRPVTSAAARARVTAARAAMAERLAGTGWRTNSEVPGSWLRA
                     RPRRLAPSATAPLDRALERGVLTMRGYDRVLRLAWSVADFDGVTRPGPSEVGAALYFR
                     GGAL"
     gene            complement(2565..2924)
                     /locus_tag="VFQ96_04635"
     CDS             complement(2565..2924)
                     /locus_tag="VFQ96_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU0257108.1"
                     /translation="MARKDVLGRRGEDLAGHYLTAQGYRILARNWRCPSGEIDLVALD
                     AEGTVVVVEVKTRSSLAFGAPLEAITAQKLARLRRLAAEWCRTHEVSGPLRIDAVGIL
                     APPGARVRLEHLRGVSR"
     gene            complement(3095..3418)
                     /locus_tag="VFQ96_04640"
     CDS             complement(3095..3418)
                     /locus_tag="VFQ96_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEU0257109.1"
                     /translation="MDEDELDDYDRQLELALYREYRDVVSQFTYVIETERRFYLANEV
                     EFARRDTDHDFYFELVMRDVWVWDVYRADRFVKSVRVLTFKDVNIEELSSKEFELPKD
                     LALDE"
     gene            complement(3432..>3712)
                     /locus_tag="VFQ96_04645"
     CDS             complement(3432..>3712)
                     /locus_tag="VFQ96_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU0257110.1"
                     /translation="RALAECPVVTRVKADRDCGSVAAASVVAKVRRDALMTALSSRHP
                     AYGWAANKGYGTAAHRAALTVHGPTPWHRRSWLHPGPGVKTPPDGESA"
BASE COUNT          564 a         1318 c         1376 g          454 t
ORIGIN      
        1 cgacggcggg caagacagcc tcggcgggag cgagcccggc cagctcggcc atgtcgtcgg
       61 ggcaggtgac gagctgggcg tcgtattcgc gaatgagtcg gtgacagccc gccgacgcag
      121 ggctcgtcac aggccccggc acggcaccga gcgcgcggcc gagctcggcc gcgtggccgg
      181 cagtgttgag ggaacccgag cgcgcacccg cctcgaccac gaccgtcgcg gtgctgcacg
      241 ccgcgatgag ccggttgcgc aacaggaatc gccagcgggt gggcgaaaaa ccgcacggca
      301 tctctgccac gacgacgccg tctcggatga tgcccgccaa caattctgcg tgcccgctcg
      361 ggtagagctt gtcgatgccc ccggcgagga tcgccacggt gaggccggcg gacgcgagcg
      421 tcgcgcgatg cgccatgccg tcgatgccgt aggcggcccc ggagacgacg gcgagcccgc
      481 ggtcggccgc gccggaggcg agttctgcgg cgaccgcggt gccgtagccg gtcgcggccc
      541 gggcgccgac cagcgcgacg gcgtgcgaca gaccgacgag ccgtgtggcg tcgccgcgca
      601 cccacaggcc cgccggggcg tggtcgccca ggtcgtcgag gccggcgggc cacccggcgt
      661 cgcccggcac gaggaacgac gcgccgaggt gcgccgcgtg ccggaacgcg tcgatcgcgg
      721 ccttggacga cagtcgggga cgccaccgct ccaaggccgc aggcagcgtg tgcgcaaggc
      781 tcgccgcgtc ttcggggtcg tcgacgaggc gggaaacggt acgggcgtcg tcgcgacgaa
      841 cgacgtggct cagcgcgcga agcggcccga gggcgcggag gagaagaccg gctgcggcgt
      901 cgcccggctc cgcgatcatc gtgaacacgc ctcgggcgaa tcgttcgccg gcctcggcag
      961 ggcccgccgt cgcctcgttc gcgaccgcgt cgaccgcgag gcgcacggcg gcctcgtcga
     1021 gggcaagaat cacagcgcac cgccccgaaa gtagagcgcg gcgccgacct cggagggccc
     1081 gggtcgggtg acgccgtcga agtcggcgac actccacgcc aggcgaagca cccggtcgta
     1141 cccgcgcatg gtgagaaccc cgcgttcgag cgcccgatcc agaggcgccg tggccgaagg
     1201 agcgagccga cggggccggg cgcgcagcca gctgcccggc acctcggagt tggtgcgcca
     1261 cccggtgccg gcgagccgct cggccatcgc ggcgcgggcc gcggtgacgc gcgcccgcgc
     1321 cgcggcgctc gtcaccggcc ggtccccggc cccgagcctc agctgcgcca ccgtcacccg
     1381 gtcaaggcag aggcgcatat cgacgcggtc gagaagcggc ccggaaagcc gggcgaggta
     1441 ccggcggcgc gcattcggcg cgcagctaca ctcccgcccg gtgccgtagt tgccgcacgg
     1501 acagggattc gccgcaagga cgagctggaa gcggcccgga aagtaggcga cggcgtcggc
     1561 ccggtgcacg gcgatccgcc ccgattccag gcattcacgc aaggaatcga gaacggcccg
     1621 gggaaactcg ggagcctcgt cgagaaaaag cacaccgtgt gccgcccggg tggcggcacc
     1681 cgggcgaatg cgaccgccgc tgccgccgcc gaccatagcc gccaccgtcg tcgcgtgatg
     1741 gggcgcctga aagggcggcc gtcgggcgag gcgccccccg accccttccc cgaggatcga
     1801 cgccaccgag gtcacctcca aggcttccgc gtcatcgaga tcgggcaaaa ggcccgggag
     1861 tctggacgcg agcatcgtct tgcccgcgcc gggcgggccc aagaggaaca ggtgatggcc
     1921 cccggcggcg gcgatctgca aggcccgcac cgcgtcgggg ttgccgatga tctcggcaag
     1981 gtcgccgccc ccggcggtgt cggtcggcac cggtgtgacg ggcgccgaca acggctccac
     2041 cggcaccggc tccagttccg cgccgtgccg gatcgctgcc tctcgcagag agggcacggc
     2101 gacgatctcg atgccgggca cgagcgccgc ctcgccggcg ttcgcggtcg gcaccatcac
     2161 ggtgtgaaag cccgaacggc gtgcagcaag gacggccggc agcacgccgg ccgtcgggcg
     2221 cagccgcccg tcgagcccca gttcgccgat gtggacgacc ccggcgacgg cgtcggggcg
     2281 taccgcccct cccgccgcca gcgctgccag ggcgacggcc aggtcgaacc ccgcgccatg
     2341 cttcgggacc gaggccggcg acaggttgac ggtgagcttg tgggtcgtca aggcacaacc
     2401 ggaattgacg gcggcggcgc gcacccggtc gcgggcctcg gcgagggccg cgtcgggcag
     2461 cccgatgatc gtgaagaacg gcagccccgc ggtaatgtcg gcctccacct cgacgagggt
     2521 gccccgcagg ccgttcaggg tgaccgccca ggttcgcgca acgctcaccg cgacaccccc
     2581 cggaggtgtt cgaggcgcac gcgggccccg ggcggcgcga ggatgccgac cgcgtcgatt
     2641 cgcagcgggc cggacacctc gtgggtgcga caccactcgg ccgcgaggcg gcgcagccgc
     2701 gccagcttct gggcggtgat cgcctcgagc ggggcgccga aggccagcga gctgcgggtc
     2761 ttcacctcga ccacgacgac ggtgccctcg gcgtcgagag ccacgaggtc gatctcgccc
     2821 gacgggcagc gccagttacg agcgagaatc cggtaaccct gagcggtcag atagtggccg
     2881 gcgagatctt cgccgcgcct gccgagaacg tctttgcgtg ccataccgcc tcccacatcg
     2941 acgttctcgc ggcaggaggg acgctgggga gggttcggcg tgatggcggc cggaaccggt
     3001 gctcggttcg cctgtgcagg agcgggatcc cgcgcgcgag gcatgcggct cccgtgacga
     3061 ccatcgcagt gatcggtgcg ggccgtgccg cgccctactc gtcgagggcg aggtctttcg
     3121 gcagctcgaa ctccttgctg gagagctctt cgatgttgac gtctttgaac gtgagcacgc
     3181 gcacgctctt cacgaaccgg tcggcccggt agacgtccca cacccagacg tcgcgcatga
     3241 ccaactcgaa atagaagtcg tggtccgtgt cgcgcctggc gaactccacc tcgttcgcga
     3301 ggtagaagcg acgctccgtc tcgatcacgt aggtgaattg ggagacgacg tcgcggtact
     3361 cgcgatagag cgccagttct agctgccggt cgtaatcgtc gagctcgtct tcgtccatga
     3421 tcctgccaag tttaggccga ctccccgtcg ggcggggtct tgacgccggg cccggggtgc
     3481 agccaactgc ggcgatgcca gggcgtcggc ccgtgcacgg tgagagccgc acggtgcgcc
     3541 gcggtgccat aacccttgtt ggccgcccag ccgtaggccg gatgacggga ggagagcgcg
     3601 gtcatgagcg cgtcgcggcg caccttcgcg acgacggagg ccgcggcgac cgacccgcag
     3661 tctcgatctg ccttcacgcg ggtgacgacg gggcactcgg ccagggcccg gc
//
LOCUS       DASTNL010000148         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_102762, whole genome shotgun sequence.
ACCESSION   DASTNL010000148 DASTNL010000000
VERSION     DASTNL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102762"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFQ96_04650"
     CDS             <1..623
                     /locus_tag="VFQ96_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEU0257111.1"
                     /translation="STTKDNALDSITETQQIPPGQLTRQSVSVAVDQAAAAKNGVTPQ
                     QVNSLVSAAAGINAKRGDAVSVQFVNFSKANALAAKTALAQAAAQARAQQIQAYIRDG
                     AIALGILLAGFFVYLGWRRSRRSASQELVPTGIDGIIPNLPPAHPAVPEFQAVPTSPA
                     PQAPGAGDPGALDARRRRSEIEAMAENDPKKAAEYLRGLMDDRTTA"
     gene            620..1645
                     /gene="fliG"
                     /locus_tag="VFQ96_04655"
     CDS             620..1645
                     /gene="fliG"
                     /locus_tag="VFQ96_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEU0257112.1"
                     /translation="MSDTKSAVPLTGAQKAAVVLMNLDQEAAAHIMQELTEVESEEIA
                     AEILRLKRVDADIADAALTEYHDLALHSAGRLRGGRDVATGLLEASFGQEKAAGVLSR
                     VASSLAGRAFEFLEQADAGQVALLLDGELPQTAAVVLAHMSPDQASAVLDILDVEFRV
                     EAAHCLATMSKASPEAVRVVAETLRARAGAVVPNREQVEPAGGVQPLVDIINRSSVAS
                     ENALLDALGRRDPELAEEVRSRMLTFSDIVKLEARDVQQVLRGIDTAVLALAMKGAPE
                     TVQETIRANISERNRAALDEEMEVMGAQRLSKVEDARSEVVRAIRQLDAEGVISVHRG
                     DEGEYVE"
     gene            1635..2258
                     /locus_tag="VFQ96_04660"
     CDS             1635..2258
                     /locus_tag="VFQ96_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEU0257113.1"
                     /translation="MSSEAFTPLSYPRLRGDNVAARAQAVADGYAEGLGRAAREEAAA
                     RKAAREAEAAARKAQAGRVASALTALHQAVERLDARLAPVAASADRALVQAALGLAEA
                     VLRREVAQGHANAEDALRRVLDTVPSGRIVTVHLNPNDLDTLRAAHLAEAAVAPASTL
                     VLDDGLRIVADAEVDPADATAELRHGWLDARIGAALARAREVLEADL"
BASE COUNT          370 a          888 c          825 g          316 t
ORIGIN      
        1 agagcaccac caaagacaac gcgctcgaca gcatcaccga gacgcagcag atcccgcctg
       61 ggcagctcac ccggcaaagt gtttccgttg ccgtcgacca ggctgccgcc gcgaagaacg
      121 gcgtcacgcc gcagcaggtc aactccctcg tctcggccgc cgccggcatc aacgcgaaac
      181 gcggcgacgc cgtctcggtg cagttcgtga acttctcgaa ggccaacgct cttgccgcca
      241 agacagcgct tgcgcaggcc gccgcccagg cgcgggcgca gcagatacag gcgtacatcc
      301 gcgacggcgc gatagcgctc ggcatcctcc tcgccggctt cttcgtctac ctcggctggc
      361 ggcgctctcg ccgcagcgcc tcccaagagc tcgtgcctac cggcatcgac ggcatcattc
      421 cgaacctgcc gcccgctcac ccggccgtgc ccgagttcca ggcggtgccg acctctcccg
      481 ccccgcaggc gcccggggcg ggagaccccg gtgcgctcga cgctcgccgt cgccgctccg
      541 agatcgaggc gatggccgag aacgacccga agaaggccgc cgagtacctg cgcggcctca
      601 tggatgaccg gaccaccgcg tgagcgacac caagtctgcc gtgcctctca ccggcgcaca
      661 gaaggctgcg gtcgttctca tgaacctcga ccaagaggcc gccgcgcata tcatgcagga
      721 gctcaccgag gtcgagtcgg aggagatcgc cgccgagatc ttgcgcctga agcgcgtcga
      781 cgcagacatc gccgacgcag cgttgaccga gtatcacgac ctcgccttgc attccgccgg
      841 ccggctgcgt ggcggccgtg acgtcgccac cgggctcctc gaggcatcct tcgggcagga
      901 gaaggccgcc ggcgtgctct cccgcgtcgc ctcctcgctc gcgggccggg ccttcgagtt
      961 tctcgagcag gccgacgccg ggcaggtcgc gctcctgctc gacggagagc tgccgcagac
     1021 tgcggccgtc gtgctcgccc acatgtcgcc cgaccaggcc tccgccgtgc tcgacattct
     1081 cgacgtggaa ttccgggtcg aggcggcgca ctgcctggcc acgatgagca aggccagccc
     1141 cgaagcggtg cgcgtcgtcg ccgaaacgct gcgggcccgt gccggggccg tcgtgccgaa
     1201 cagagagcag gtggagccgg ctggcggggt gcagccgctc gtcgacatca tcaaccgctc
     1261 cagcgtcgcc agcgagaatg ccctgctcga cgcgctcggc cggcgggatc ccgaactggc
     1321 cgaagaggtg cgcagtcgca tgctcacctt cagcgacatc gtcaagctgg aggcccgaga
     1381 cgtgcagcag gtgctgcgcg gcatcgacac cgcggtgctc gccctcgcca tgaaaggcgc
     1441 ccccgagacc gtgcaagaga cgattcgcgc caacatctcg gagcgcaaca gggcggcgct
     1501 cgacgaagag atggaggtga tgggcgccca acgcctctcg aaggtcgaag acgcccgctc
     1561 cgaggtggtg cgtgccatcc ggcagctcga tgccgaaggc gtcatctctg tgcatcgcgg
     1621 cgacgagggt gaatatgtcg agtgaggcgt tcacgccgct ctcgtacccg cgtcttcgcg
     1681 gcgacaacgt cgccgcccgg gcccaggccg tcgccgacgg gtacgcggag ggtcttggcc
     1741 gtgcggcccg ggaagaggcg gcggcgcgca aagccgcccg cgaggcagag gccgcggccc
     1801 gcaaggctca ggcgggacgc gtcgcgtccg cgctcacggc gcttcaccag gccgtcgaac
     1861 ggctcgacgc gaggctggca cccgtcgcag ccagcgccga tcgcgcactc gtgcaggccg
     1921 cgctcggcct ggccgaggcg gtcttacgcc gagaggtcgc ccaggggcac gcgaacgctg
     1981 aagacgcgtt gcgccgggtg ctcgacacgg tgccgtcggg ccgtatcgtc accgttcacc
     2041 tgaacccgaa cgacctcgac acgctgcgtg ccgcccacct cgccgaggcg gccgtcgcgc
     2101 cggcctcgac gctcgtgctc gacgacgggc tgcgcatcgt ggccgacgcc gaggtcgacc
     2161 ccgccgacgc gacggcggaa ctgcggcacg gctggctcga cgcccgcatc ggtgcggctt
     2221 tggcgcgcgc ccgcgaggtg ctggaggccg acctgtgacc cggggccccg gcttcggcac
     2281 gatcgcgcca ggcatcgcgg gcaccgttgc gggcacaggc ccgcggcagc gcgccggcga
     2341 aggcgtgccc ggtacccgcg gccttctcga aggcgcaggg ttccgggccg cgctcgacg
//
LOCUS       DASTNL010000149         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1583555, whole genome shotgun sequence.
ACCESSION   DASTNL010000149 DASTNL010000000
VERSION     DASTNL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583555"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1240
                     /gene="galT"
                     /locus_tag="VFQ96_04665"
     CDS             119..1240
                     /gene="galT"
                     /locus_tag="VFQ96_04665"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU0257114.1"
                     /translation="MGQIRKRATRLADGRELIFFDDADTTLPEGRKADARQLEPRPQT
                     ATMRHDVLTGEWVSIASARQNRVFLPPAELDPLAPATPSNPAEVPDQYDVAVFENRTP
                     SFGPAMTAADAPKGLADLAQTGLGLTRASVGRCEVVCFSPESTGSFGSQTRSRARTVI
                     EAWADRTRELGAMAGIEQVFPFENRGKEIGVTLGHPHGQIYAYPYITPRTTRLLAQID
                     AYGPSLFADILDFERAGERLVVSGRHWSAFVPFAARWPVELHMLPHRQVPDFAATSAE
                     ERDELAVIYLRLLRAVDTLYDTPTPYIAAWHQAPVHAGRETVRLMLQLTSPRRAADKL
                     KYLAGSEAAMGAWIGDVTPESGAQALRDALARADVENPA"
     gene            1237..2403
                     /gene="galK"
                     /locus_tag="VFQ96_04670"
     CDS             1237..2403
                     /gene="galK"
                     /locus_tag="VFQ96_04670"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU0257115.1"
                     /translation="MTGPGGVRAAFTERFGARPEGVWSAPGRVNLIGEHTDYNDGFVL
                     PFAIDRRVVAAVRRRDDNVLRVASTFADEAVEVPLAGLGESGAARVSGWAGYLVGVAW
                     ALGRQGVSLADQQGLDIVIDSDVPVGAGLSSSAAIECAVAVAMNDLWALHLPLESLVD
                     AGHEAENTFVGAPTGILDQSASLLAARDHAVFLDCRSRHTELIPLGLADAGLTITVID
                     TGVRHAHADGAYRERRASCEQAARLLGVAALRDVTLDELRRSRDRLDPVTFRRARHIV
                     TENDRVLRTVDTLRHEGVAAIGDLLDASHRSMRDDFEISVPELDLAVETAQNFGAPGA
                     RMTGGGFGGSAIALTPREAVSRVEAGIAGAFAEHGYTPPVIFTVTADAGARRDA"
     gene            2476..3012
                     /locus_tag="VFQ96_04675"
     CDS             2476..3012
                     /locus_tag="VFQ96_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU0257116.1"
                     /translation="MIDPRALLIPIAGAHPLVAPTAWVAPGAVLAGNVTLAAQASVWY
                     TCVLRSEGEAIVVGRRTNLQDGTICHVDDGYPLTLGSGVSVGHQAVLHGCTVDDDVLI
                     GMGARVLTGAVIGRESLVAAGSVVLEGTVVPARSLVAGVPARVHRALTDDEVAGIRRN
                     AQTYLAHTALHAATPDAA"
     gene            complement(3124..>3708)
                     /locus_tag="VFQ96_04680"
     CDS             complement(3124..>3708)
                     /locus_tag="VFQ96_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0257117.1"
                     /translation="RSASGISSRFHQAPYGVFQTADGWLTLSLADGATLARAFDDDRL
                     AGFTREDQFDRREEINGILAEHMATKTTAHWRSHLDGIGVWNAPANEYDDMLREEGGQ
                     LAANETILTVEHPSAGTIRLLGHPVKYDGHVPAVRRPPATVGQHTGEVLSEAGYSDDE
                     IARLREAGAVGPDRAVVPFDRKASEPKSAYSGKH"
BASE COUNT          521 a         1365 c         1292 g          530 t
ORIGIN      
        1 gctcatgcct ctaaccttag gggcgccgat gccgcgccac gcgagggcgt gccggggcca
       61 cccggcacct gcgtatcctg gctttcggca cagaaaactt cgaggcgcgg aggcgggcgt
      121 ggggcagatc agaaaacggg cgacgcggct ggcggacggc cgggaattga tcttcttcga
      181 cgacgcagac acaaccctgc ccgagggccg caaggctgac gcgcggcagc tggagccgcg
      241 accgcagacg gcgaccatgc ggcacgacgt cttgacgggc gagtgggtct cgatcgcctc
      301 ggcacgacag aacagggtgt tcctgccgcc ggcagaactc gacccgctgg cgccggcgac
      361 accgtcgaac ccggccgagg ttcccgatca gtacgacgtg gccgtgttcg agaaccgcac
      421 gccgtcgttc ggcccggcga tgacggccgc cgacgcgccg aaaggcctgg ccgacctcgc
      481 gcagacgggg ctcggcctca cccgggcctc ggtggggcgc tgcgaggtgg tgtgtttcag
      541 cccggaaagc accggatcgt tcggcagcca gacccgctcc cgcgcccgca ccgtgatcga
      601 ggcctgggca gatcgcaccc gggagctcgg cgccatggcc ggcatcgagc aggtgttccc
      661 gttcgagaac cgcggcaaag agatcggcgt cacgctcggg cacccacacg gccagatcta
      721 cgcgtacccg tacatcacgc cacgcaccac acggctcctg gcccagatcg acgcgtacgg
      781 gccgagcctc ttcgccgaca tcctcgactt cgagcgcgcc ggggaacgcc tcgtcgtctc
      841 aggtcggcat tggagcgcgt tcgtgccgtt cgcggcgcgc tggccggtcg agctgcacat
      901 gctgccacac cggcaggtgc ccgacttcgc cgcgacgagc gccgaggagc gcgacgagct
      961 cgccgtcatt tacttgcggc tgctgcgcgc cgtcgacacc ttgtacgaca cgcccacgcc
     1021 gtatatcgcg gcctggcacc aggcgccggt gcacgccggc cgtgagacgg tcaggctcat
     1081 gttgcagctg acaagcccgc gccgggcggc agacaagctg aagtacctcg ccggcagcga
     1141 ggccgcgatg ggcgcctgga tcggcgacgt gacgccggag tcgggcgccc aggcgctgcg
     1201 cgacgcgctc gcccgtgccg atgtggagaa cccggcgtga cgggcccggg cggcgttcgg
     1261 gccgccttca ccgaacggtt cggcgcccgg ccggagggcg tctggtcggc gcccggccgg
     1321 gtgaacctga tcggcgagca cacggactac aacgacggat tcgtgctgcc gttcgcgatc
     1381 gaccggcgcg tcgtcgctgc ggtccgacgc cgcgacgaca acgtcttgcg ggtggcgagc
     1441 acgttcgccg acgaggccgt cgaagtgccg ctggcggggc tcggtgagtc gggcgccgcc
     1501 cgcgtctcag gctgggccgg ctatctcgtc ggcgtggcat gggccctcgg ccggcaaggt
     1561 gtgagcctcg ccgaccagca aggcctcgac atcgtcatcg actccgacgt gccggtcggc
     1621 gccggcctgt cgtcgtcggc ggcgatcgaa tgcgcggtcg ccgtcgccat gaacgacctg
     1681 tgggcgctgc acctgcccct cgagagcctt gtcgacgcgg gccacgaggc ggagaacacg
     1741 ttcgtcggcg cgccgaccgg cattctcgac caatccgcct cactcctggc ggcccgggac
     1801 cacgccgtct tcctcgattg ccgcagccgc cacaccgagc tgataccgct cggcctggcg
     1861 gatgcaggcc tcaccataac cgtgatcgac acgggcgtgc gacacgcgca cgccgacggc
     1921 gcctaccgtg agcgccgggc atcctgcgaa caggccgccc gcctcttggg agtcgccgca
     1981 ctgcgcgatg tcaccctcga cgagctccga cggagccgag accggctcga tcctgtcacg
     2041 ttccgccgcg cccggcacat cgtgaccgag aatgaccgcg tgctgcgcac cgtcgacacg
     2101 ctccggcacg agggtgtggc ggcgatcggc gacctgctcg acgcctcgca ccgttcgatg
     2161 cgcgacgact tcgagatctc cgtgccagag ttggacctcg ccgtcgagac cgcgcagaac
     2221 ttcggtgcac caggcgcccg catgacgggc ggcggtttcg gcggctcggc catcgcgctg
     2281 acgccccgcg aggccgtctc ccgcgtcgaa gccggcatcg cgggggcctt cgctgaacac
     2341 ggctacaccc cgcccgttat cttcacggtc accgccgacg ccggggcgag gcgcgacgcc
     2401 tgagcgccgg gcgacggatc ccgaggagcc ggagcaggcg ttcaggcgag cgccagccgg
     2461 cgatacgctg agacgatgat cgaccctcgc gccctcctca tccccatagc cggcgcgcac
     2521 ccgctcgtcg caccgaccgc ctgggtggcg ccaggcgccg tgctcgccgg caacgtgacc
     2581 ctcgccgccc aggccagcgt ctggtacacc tgcgtgctgc gcagcgaggg cgaggcgatc
     2641 gtcgtcggcc ggcgcaccaa cctgcaagac ggcacgatct gccacgtcga cgacgggtac
     2701 ccgctgacgc tcggctccgg cgtctccgtc gggcatcagg cggtgctgca cggttgcacc
     2761 gtcgacgacg acgtgctcat cggcatgggc gcccgcgtct tgaccggcgc cgtcatcggc
     2821 cgcgaatccc tcgtcgccgc gggctcggtc gtcttggagg gcacggtcgt gccggcccgc
     2881 tccctcgtcg ccggcgtgcc ggcccgagtg catcgcgcgc tgaccgacga cgaggtggcc
     2941 ggcatccgcc gcaacgcgca gacgtacctc gcccacacgg cgctgcacgc cgccaccccc
     3001 gacgccgcct gaccggccca cccgcggggc cgcgggcctg cgcgcttgcc ttttgccggg
     3061 ccggacctca cctcagcggt tgcctgactt cagcggttgc ctgatttcgg cgcccgccgg
     3121 gcttcagtgc ttgccggagt aggccgactt cggctcggag gccttgcggt cgaacggcac
     3181 gacggcacgg tcggggccga ccgcgccggc ctcgcgcagc cgggcgatct cgtcgtcgct
     3241 atagcccgcc tcgctcagga cctcgccagt gtgctgcccg acggtcgccg gcggccggcg
     3301 caccgccggc acgtgcccgt cgtacttgac cggatgcccg agaagccgga ttgtgccggc
     3361 cgacgggtgc tccaccgtca agatcgtctc gttggccgcc agctgcccgc cctcctcgcg
     3421 cagcatgtcg tcgtactcgt tcgcgggtgc gttccagact ccgatgccgt cgagatggct
     3481 ccgccagtgc gccgtcgtct tcgtcgccat gtgttcggcg aggatgccgt tgatctcttc
     3541 gcgccggtcg aactggtcct cccgagtgaa accggcgagg cggtcgtcgt cgaaggcgcg
     3601 cgccagggtg gcgccgtcgg cgagcgacag ggtgagccag ccgtctgcgg tctgaaagac
     3661 gccgtaaggc gcctgatgga accgggacga gatgccggag gcactgcg
//
LOCUS       DASTNL010000150         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_105305, whole genome shotgun sequence.
ACCESSION   DASTNL010000150 DASTNL010000000
VERSION     DASTNL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105305"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFQ96_04685"
     CDS             <1..1238
                     /locus_tag="VFQ96_04685"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU0257118.1"
                     /translation="LRVLKTLSRTQWDVGRAFGTVGARVAGENVEITTYRADRYDGAT
                     RKPDVVFGDDIRQDLARRDFSINALAFLLPQRRLIDPSGGIDDLIAGRLRTPGPPEVS
                     FGDDPLRMMRAARFAAQLGFGVDALTRAAMTRMSARLDIVSQERVRDEVVKLLSADAP
                     RPGLELLVETGLAERVLPELPALQLEIDEHHHHKDVYRHTLTVVDQAIDYERRRHPGE
                     PPDVVVRLAALLHDIGKPATRRFEPHGAVSFHHHDVVGAKLAKARLRTLRFDNDTIRA
                     TCRLIELHLRFFGYADQAWTDSAVRRYVHDAGDQLGRLHILTRADVTTRNRRKADMLA
                     FAYDDLEARIAQLAKEEDLAARRPDLNGEEIMALLDIPPGPEVGRAYRYLLDLRINEG
                     PLGREEATRRLLDWRAKDA"
     gene            1231..2190
                     /locus_tag="VFQ96_04690"
     CDS             1231..2190
                     /locus_tag="VFQ96_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0257119.1"
                     /translation="MPEPVARRRGVALAPSARRTAEAAPTPRRHRGLVVRRSLTGWGL
                     LAPSLVGIVVFLAVPIGVVVWLSTQKWNLITPPSFVGAANWGAILADPAFQTSVVVTL
                     LFVIMVIPVQTALGLLVAVLLTRRLPGSAVFRVIFLIPWACAPLALGIVWQWIFAPTG
                     GALNALLGTNVPWLTEPAIALPAVALVTVWSQVGYIALFFTAGIAGIPEQIIDAARVD
                     GAGEARIFFSVTLPLLRPTMFFVLVTSVISTFQVFDSVFALTGGGPQNTTDVIAYELY
                     QRAFVDFDMGKAAVIAVVLFVVLAALTVAQHLWFRRKITYDLS"
     gene            2177..3097
                     /locus_tag="VFQ96_04695"
     CDS             2177..3097
                     /locus_tag="VFQ96_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0257120.1"
                     /translation="MTSVDEAAAAVGTERSGIAAGTAGRRPRRRAARHIPLALGAYAI
                     LAAGALLTLGPFVLSAMTSLKTPRQFATQSALRAPDPATLQNYTGLFATDVADFGRSF
                     AVTALVVVTVLVGQMVFSIMAAYAFARIRFPGRDAVFWVYLSTLMVPPVVTVVPLYLM
                     MSQAGLRNTFWALVLPYLFGSPYAIFLLREYFRGIPQDLVDAARIDGAGTWRILVQLV
                     VPLSRPIIVTLAVITVVTHWNNFLWPLIITSGQTWRVVTVSTASLQSQYNGNWTLVMA
                     ATTLAIAPLLVLFLLFQKQIVRSIAVTGFK"
     gene            3137..4501
                     /locus_tag="VFQ96_04700"
     CDS             3137..4501
                     /locus_tag="VFQ96_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU0257121.1"
                     /translation="MKRSRMVLAAAVLAAATLAVTGCSSASSQDSGAKGSAASPTTVT
                     LRTWDTNAAAAYKESFAEFTHDNPSIKVKVDVVPWANYFTTLRTDIAGGNADDIFWIN
                     SSSYSPYASGGKLINIDQALGKAAKKAWAPSVVQQFTQGGSLWGVPQTSDGGIALYYN
                     KDLVKKAGLTESDIAHLTWSPAGGTGPDDTLLTVAQKLTRDQSGKTANEKGFEAAKTT
                     QWGYSASQDLQAIYFPFIGSNGGTFQNQKTGAFTFATPKTEAAFQYIVDLINKYHVAP
                     SAADTNTDGNFTLNQFQQGKIALFQSGLYNLSNVADNVKFPWGVVMQPAGPAGDVSVT
                     NGIAAVGNADSPHLAATKKVLKWIGSREGNQFIGATGANLPAVTAAQQTYFDYWKGQG
                     VDVTPFFQVIKDKPTIPAPFGAGFAAASTAYKPIFNEIFLGRTPVKAGLEEAQAAANK
                     AFTG"
BASE COUNT          710 a         1651 c         1454 g          785 t
ORIGIN      
        1 tgctccgtgt cttgaaaacg ctctctcgca cgcagtggga cgtggggcgc gccttcggca
       61 ccgtcggcgc ccgggtcgcc ggcgagaacg tcgagatcac gacctatcgg gccgaccgtt
      121 acgacggtgc gacgcgtaag cccgacgtcg tcttcggcga cgacattcgg caagacctcg
      181 cgcggcgcga cttctcgatc aacgcgctcg cctttctctt gccgcagcgt cgcctgatcg
      241 atccgtcggg cggtatcgac gacttgatcg cgggccggct gcgcaccccg ggcccccccg
      301 aggtctcgtt cggcgacgac ccgttgcgca tgatgcgggc ggcgcgcttc gccgcccagc
      361 tgggcttcgg cgtcgacgcc ctgacccgcg ccgcgatgac ccggatgagc gcacgtctcg
      421 acatcgtcag ccaggagcgg gtgcgcgacg aagtcgtgaa gctcctctcc gcagacgccc
      481 cgaggcccgg cctggagctt ctcgtagaga cgggcctcgc cgagcgggtg ctgcccgagc
      541 tgccggcgct ccagctcgag atcgatgagc accatcatca caaagacgtc tatcggcaca
      601 ccctcacggt cgtcgaccag gcgatcgact acgagcgcag gcgtcatccg ggcgagccgc
      661 cggacgtcgt cgtgcgcttg gccgccctgc tgcacgacat cggcaagccg gccacgaggc
      721 gcttcgagcc gcacggcgcc gtcagcttcc accaccacga cgtcgtgggc gccaagttgg
      781 cgaaggcgcg gcttcgcacc ctgcgcttcg acaacgacac catccgggcg acctgtcgtc
      841 tcatcgagtt gcacttgcgc ttcttcggct acgccgacca ggcgtggacg gattcggccg
      901 tgcgccgcta cgtgcacgac gccggggatc agctcggtcg cctgcacatt ctgacgcggg
      961 ccgatgtgac gacccgcaac cggcgcaagg ccgacatgct ggccttcgcc tatgacgacc
     1021 tcgaggcgcg gatcgcgcag cttgccaagg aggaagacct ggcggcgagg cggcccgacc
     1081 tgaatggcga ggagatcatg gcgcttctcg acattccgcc cgggccggag gtcggccggg
     1141 cgtaccgcta cctgctcgac ctcaggatca acgaggggcc gttggggcgc gaggaggcga
     1201 cgcgccggct gctcgactgg cgtgcgaaag atgcctgagc ccgtcgcccg acgccggggc
     1261 gtggcactgg cgccgtcggc ccgccgcacg gcggaggcgg cacccacgcc acggcgtcac
     1321 cgcgggctcg tcgtgcggcg cagcctgacg ggctggggtc ttctcgcccc gagcctcgtc
     1381 ggaatcgtcg tcttcctcgc ggtgccgatc ggcgtcgtcg tctggctgag cacccagaag
     1441 tggaacctca tcaccccgcc gagcttcgtc ggtgcggcca attggggggc gatcctcgcc
     1501 gaccccgcct ttcagaccag cgtcgtcgtg acgctccttt tcgtcatcat ggtgatcccc
     1561 gtgcagaccg cgctcggcct tctcgtcgcg gtgcttctga cccggcggct gcccggctcc
     1621 gccgtgttcc gggtcatctt cctcatcccg tgggcgtgcg cgccgctcgc gctcggcatc
     1681 gtgtggcaat ggattttcgc gccgaccggc ggcgccttga acgccctgct cggcacgaac
     1741 gtgccgtggc tcaccgagcc ggcgatcgcg ttgccggccg tcgcgctcgt caccgtctgg
     1801 tcgcaggtcg gctacatcgc cctcttcttc acggcgggca tcgccggcat cccggaacag
     1861 atcatcgacg ccgcccgcgt cgacggggcc ggcgaggccc gcatcttctt ctccgtcacc
     1921 ctgccgcttc tgcgcccgac gatgttcttc gttctcgtca cgtcggtgat cagcaccttt
     1981 caggtcttcg acagcgtgtt cgccctgacg ggcggcgggc cgcagaacac gaccgacgtg
     2041 atcgcctacg agctgtacca gcgcgcattc gtcgatttcg acatgggcaa ggccgccgtc
     2101 atcgcggtcg tgctcttcgt cgttcttgcc gccctcaccg tggcacaaca tctctggttc
     2161 cgccgaaaga tcacctatga cctcagttga cgaggcggcc gccgccgtag gcaccgagcg
     2221 ctcggggatc gccgccggaa ccgcgggccg gcgcccgagg cgccgcgcgg cgcggcacat
     2281 ccccctcgcg ctcggcgcct acgccatcct cgcggccggc gcgcttctca cccttgggcc
     2341 gttcgtcttg agcgccatga cctcgttgaa gacgccacga cagttcgcca cacagtccgc
     2401 gcttcgggcg ccggacccgg cgaccctgca gaactacacg gggctcttcg cgaccgacgt
     2461 cgccgatttc ggccgctctt tcgccgtcac cgcgctcgtc gtcgtgacgg tcttggtcgg
     2521 acagatggtc ttctcgatca tggcggcgta tgccttcgcg cgcatacgtt tcccgggccg
     2581 cgacgccgtg ttctgggtct acctctccac cctcatggtc cctccggtgg tgaccgtcgt
     2641 cccgctctac ctgatgatga gccaggcggg gctgcgcaac acgttctggg cgctcgtctt
     2701 gccctacctg ttcggctcgc cctatgcgat cttccttctg cgcgaatatt tccgcggcat
     2761 accgcaagat ctcgtcgacg ccgcccgcat cgacggcgcc ggcacctggc gcattctcgt
     2821 tcagctggtc gtgccgctct cccgcccgat catcgtgacc ctcgccgtca tcacggtcgt
     2881 gacgcattgg aacaacttct tgtggccgtt gatcatcacg agcgggcaga cgtggcgcgt
     2941 cgtcacggta agcactgcta gtctgcaatc gcagtacaac ggcaattgga cgctggtgat
     3001 ggcggcgacg acgctcgcga tcgcgcccct cctcgtcctc ttcctgctct ttcagaagca
     3061 gatcgtgcgc tcgatcgccg tgaccgggtt caagtgatct gcgcgccggt ttttgctcga
     3121 gtctgacgag gtggacatga aacgttcccg tatggtgctg gccgcggccg tcttggcggc
     3181 ggcgacgctc gccgtgaccg ggtgctcgtc ggcctcctct caagactccg gcgccaaggg
     3241 ttccgccgcg tcgccgacga ccgtcacgtt gcgcacctgg gacacgaacg cggccgcggc
     3301 ctacaaggag tcgttcgcag agttcaccca cgacaacccg tcgatcaagg tcaaggtcga
     3361 cgtcgtcccg tgggccaact acttcacgac cctgcgcacc gacatcgccg ggggcaacgc
     3421 agacgacatc ttctggatca actcctcctc gtattccccc tatgcctctg gcggcaagct
     3481 catcaatatc gaccaggcgc tgggcaaggc ggcgaagaag gcctgggcgc ccagcgtcgt
     3541 gcagcagttc acacagggcg gcagcctgtg gggcgtgcca cagacctccg acgggggcat
     3601 cgccctctac tacaacaagg accttgtgaa gaaggccggc ctgacagagt cggatatcgc
     3661 gcacctcacc tggagcccgg cgggtggcac gggcccggac gacacgctcc tcaccgtcgc
     3721 gcagaagctc acccgagacc agtcgggcaa gacggcgaac gagaagggct tcgaggcggc
     3781 caagaccacc caatggggtt actccgcctc gcaagacctg caagcgatct actttccgtt
     3841 catcggctcc aacggcggca ccttccagaa tcagaagacc ggcgcgttca cgttcgcgac
     3901 cccgaagacc gaggcggcct tccagtacat cgtcgatctc atcaacaagt accacgtggc
     3961 ccccagcgcg gccgacacca acaccgacgg caacttcacg ttgaaccagt ttcagcaggg
     4021 caagatcgcg ctgttccagt cgggcctcta caacctctcg aatgtcgccg acaatgtgaa
     4081 gttcccgtgg ggcgtcgtca tgcagccggc gggccccgcc ggcgacgtca gcgtcacgaa
     4141 cggcatcgcc gcggtcggca acgccgacag cccgcatctc gccgcgacga agaaggtgct
     4201 gaagtggatc ggctcgaggg agggcaacca gttcatcggc gcgaccggcg ccaacttgcc
     4261 ggccgtgacc gccgcgcaac agacgtactt cgactactgg aagggccagg gggtcgacgt
     4321 gaccccgttc ttccaggtca tcaaggacaa gccgacgatc cccgcaccct tcggcgcggg
     4381 cttcgccgcg gcctccaccg cctacaaacc gatcttcaac gagatcttcc tgggccgcac
     4441 ccccgtgaag gccggcctcg aagaggcgca ggcggccgcc aataaggcct tcaccggctg
     4501 acgggcagcg ggcgggcgtc gcgtgaggtg aggtacgcgg ccggcacggc gtcgtggcag
     4561 agcgcaatgt tgcgtaacga agccgcgcgt ggcacccggc
//
LOCUS       DASTNL010000151        10768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1401576, whole genome shotgun sequence.
ACCESSION   DASTNL010000151 DASTNL010000000
VERSION     DASTNL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401576"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..908)
                     /locus_tag="VFQ96_04705"
     CDS             complement(216..908)
                     /locus_tag="VFQ96_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0257122.1"
                     /translation="MEAGPRILIVDDEPNIRDLLTTSLRFAGFAVRAVANGAQTISAV
                     LEEEPDLIILDVMLPDMNGFSVTKRLRGAGYTAPILFLTAKDDTEDKITGLTVGGDDY
                     VTKPFSLDEIVARIKAILRRTMHVDEDAVIHAGELTMDQDTHEVFVGQAQVELSPTEF
                     KLLRYLMLNPNRVLSKAQILDHVWEYDFNGDAGIVESYISYLRRKLDGHSGEPLIQTK
                     RGFGYMLKVGKA"
     gene            complement(998..1738)
                     /locus_tag="VFQ96_04710"
     CDS             complement(998..1738)
                     /locus_tag="VFQ96_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0257123.1"
                     /translation="MTDDERSGAGRTGVAVEAAASLRDPARTKLYALIRTRDEAMSRD
                     DAARLTGMPRSTAAFHLDRMVESGLLEVEYRRRSGKGGPGAGRPAKLYRRARQVITLS
                     LPERRYDVVGDVLASAVERVRTTGEGIDASLAEVAREAGARAAAGHTDLLEALRVSGY
                     EPHTEQDGDVVFGNCPFDELAGNHRGLVCGLNLNLVQGMADACGCEGHIEQDPGAGRC
                     CVRAVGLDAAARPAPGRHLAGRDEEERR"
     gene            1873..2535
                     /gene="folE"
                     /locus_tag="VFQ96_04715"
     CDS             1873..2535
                     /gene="folE"
                     /locus_tag="VFQ96_04715"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0257124.1"
                     /translation="MSSTITIDEEDAALQTMTGQAPSPRQHAEVSDGSMRQVTPTIDR
                     VAAQAAVADLLRALGRDPEKESLADTPRRVADAYRELLTPVPFKATTFPNDEHYDELV
                     LVKDIPFHSLCEHHLLPFYGVAHIAYLPADRIVGLSKLPRMLEYCARDLQVQERLTVQ
                     VADWLDAILEPQAVGVVLEAEHECMRLRGVKTAGARTRTVAVRGLLKTDRALRREFLD
                     RL"
     gene            2618..3541
                     /gene="folP"
                     /locus_tag="VFQ96_04720"
     CDS             2618..3541
                     /gene="folP"
                     /locus_tag="VFQ96_04720"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0257125.1"
                     /translation="MPCPRGLYRPAIMTPVRTIGAHRFDFSRQVAVMAVVNRTPDSFY
                     DRGATFALDAAIRAALDAAHEGADWVDIGGVPFSPGEPLPIEEEAARVVPVVAGIRAR
                     SDVVISIDTFQPEVARRAIAAGADVINDTTGLHDPAMADVVAGSAASLVITHSLAAPR
                     TVYPRPHYDDIAGEVISFLRERVDRATAHGVPANRIILDPGHDLNKNTVQSLEVTRRF
                     AEIADLGFPTLAAVSNKDFIGETLGLPRGERVEGSLAAAVACVLLGARIVRMHNVRQS
                     VRAMRMVEAILGWREPAYQKHNMGDVNEPVR"
     gene            3538..4329
                     /locus_tag="VFQ96_04725"
     CDS             3538..4329
                     /locus_tag="VFQ96_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HEU0257126.1"
                     /translation="MTGTEPAAIDRLWPAPATALDDDTLAEGYEAPRLPWLRVNFVAS
                     LDGSAARDGRSGGLSDAADRRVFATLRRLCDVVVVGAGTVRAEGYGGMRVDEATAAWR
                     SEHGRAPQPRLAIVTNRLHLSATDAALAEAVARPIVITCASAPETTRRELSEVADVLI
                     CGQARVEPARLVAALAARGMPRMLCEGGPRLFGALAEAGVVDELCLTTAGVLVGGRGP
                     RILHADRQLDLSMRLAHVLAAGETLLTRYVRAPSSASSAAASRIV"
     gene            complement(4275..5933)
                     /locus_tag="VFQ96_04730"
     CDS             complement(4275..5933)
                     /locus_tag="VFQ96_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEU0257127.1"
                     /translation="MSDGPLIVQSDRTVLLEVAHPLADEARHELAVFAELERAPEHVH
                     TYRITRLGLWNARAAGHDAASMLQTLERYSKFEVPRSVVVDMTETVARYGRLTITRDE
                     DGTLVLTSDDAAVLTEVSRAKKVAPLLTGRRDATSFSVAAWARGELKRELVKLGWPAD
                     DTAGYTPGTPLEIGLVEDGWRVRDYQASAAESFAAGGSGVVVLPCGAGKTVVGLAAMA
                     QTGTTTLILVTNTVAARQWRAEIVKRTTLLPEQVGEYSGQEKEVRPVTIATYQILTTR
                     RRGEYTHLALLDALDWGLIVYDEVHLLPAPVFKLTADLQARRRLGLTATLVREDGREG
                     DVFSLIGPKRFDAPWRQIEEQGFISPASCYEVRVDLPDDERLGYAAAGDDERFRLAAS
                     APAKMAVVRTLVHKHAGARILVIGQYLDQLHELSTMLGVPALTGATPVNERERLYQEF
                     RDGVTPVLVVSKVANFSVDLPEASVAIQVSGTYGSRQEEAQRLGRLLRPKESGAQASF
                     YTLVARDTVDQDFAQNRQRFLTEQGYSYTILDAAALDAEEGART"
     gene            6093..7193
                     /locus_tag="VFQ96_04735"
     CDS             6093..7193
                     /locus_tag="VFQ96_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0257128.1"
                     /translation="MNDQTNAGSEAGETPEPLPDGVARAMLERVDAAAVFYDASRRPR
                     WYNARAAEFAALAGLDLATGRTRHVYLADRETRLTGNVDILDETLAGPGRGQLYWLGD
                     PCGTQVAVVSRAHRLETQGGGFLGWSILAYDVTETATIFVNRAEYFSAISHELRTPLT
                     SILGYLDLIIDDGGSGDDEIDRQLRIVLRRAEQLSKLVADYAELSGVGTVRAQETDLC
                     ALVTDTVRARQERAAGRERPILTRVPDRPLVCRVDPGRIAKALDNILANAVTYTPPEG
                     SIRVSVEADSTEVRIAVADEGAGIDAAEMPRIFDLFYRSPKHYRKVIQGNGIGLPIAH
                     TIVTAHHGRITVHSVPGRGATFTIHLPCGAVR"
     gene            complement(7233..9050)
                     /locus_tag="VFQ96_04740"
     CDS             complement(7233..9050)
                     /locus_tag="VFQ96_04740"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257129.1"
                     /translation="MVDIRRAEARDRLADDTSNLPGGQAHRMWVLAVLALAQLMIVLD
                     TTVVTIALPSAQHDLGFTTGDRQWIITAYALSFGSLLLFCGRLTDLLGRKTTLIIGLV
                     GFAVASAVGGAAGSFAMLVTARAVQGGFAALLAPSVLALLTTTFTSAKERGRAFGVFG
                     AVVGSGAAVGLLLGGFLTEYLSWRWCLYVNLVFAVFALGGAIILIHRPARGEGAHLDV
                     PGVLTVSAGLFALVYGFSNAVSNGKANWDDPATIGFLVAAAVLLVAFVVLQTRVRHPL
                     LPLSVLAHRYRGPAFAALLLGGAGLFGALLFVTYYVQQVLGYTALHTGVAFLPMVACL
                     MLASISSNVILIRRISPRILLPTGLTLAAAGLALLTRIGPDSTYSGVLLAPLIVIGLG
                     LGMAFSTSINLATLDVSDDEAGPASASVNTAQQIGAAVGTALLNTLAIQASSTYLSGH
                     EQALLRPIAAAKATLAAAKEAIAAAGAHGPAAVQAAHEKWSAPLAHATDVLKRAPAAM
                     HDLGTHAAIASYSAAFWWGAALFLAGAIATLLLLRTPVPPALQHAPVPKEPAAQGEPA
                     HAAQEPGGRVPEPETRSAPAPGTRAPDVSATGLGHLPDS"
     gene            complement(9109..9627)
                     /locus_tag="VFQ96_04745"
     CDS             complement(9109..9627)
                     /locus_tag="VFQ96_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0257130.1"
                     /translation="MSPATATNPAQAGRAGDPSRPPTGTDLAGMLRTSVGRLSYCLRG
                     PAAQSGLTPTRLTALVSLEKTGPVRPGQLARGLGISAASMSRLLEALQVGGWVRRQPD
                     PDDQRASLVGLSPDGTERLGKLRQEGTTELSDSLDHLDAAERAAIAAALPALTHLADT
                     YLARHRRRDEHV"
     gene            complement(9624..>10768)
                     /locus_tag="VFQ96_04750"
     CDS             complement(9624..>10768)
                     /locus_tag="VFQ96_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257131.1"
                     /translation="ARMDWWGNITFAVGLIALLVGITYGIQPYGGHTMGWTSPFVLTT
                     LVGGAVILVGFCYLETRVAQPMFRLDLFRIRAFTAGNFAGLLSALGRGGLMFILIIWL
                     QGIWLPRHGFSFVSTPLWAGIYMLPMTVGFLIAGPTSGWLSDKFGARGFATGGMIIAA
                     ASFGWLYLMPVNFSYWQFALALLVNGIGMGLFSAPNRTGIMNSLPAHQRGVGSGMSYT
                     FQNSAMVLSIGIFFTLMIVGLAASLPHTLVTGLTGQGVPAAAAAQVAALPPVAVLFAS
                     LLGYNPVQTLLGPHVLAHLSAGHAAYLTGRQFFPALISGPFSAGLAVAFGFAAAACLV
                     AAAASALRGGKYVYPDDSAAGKDTSYGPAGHEDHPSDGILTPRGGR"
BASE COUNT         1676 a         3544 c         3856 g         1692 t
ORIGIN      
        1 gctgtcgacc ttgctgtcgg tctgatccat caggtacccg cgcagcacgc tgaccgtgcc
       61 ggcgccggcc accgcgaggc cgaaggtgag cagcaggacg gtgaccccgg tgatcttggc
      121 gcgcagcgag atgtcgttcc acacccgccg cagcattccc cccatatgag gcagtttaag
      181 aggctgtcac gcgcgtggcc ggcgggtggc ctcggtcagg ctttgccgac cttcagcatg
      241 tacccgaagc cccgcttggt ctgaatgagg ggctcgccgg agtggccgtc gagcttgcgg
      301 cgcaggtaag agatgtaact ctcgacgatg ccggcgtcgc cgttgaagtc gtattcccac
      361 acgtggtcga gaatctgtgc cttgctcaac actcggttgg ggttcagcat caggtagcgc
      421 agcagcttga actcggtcgg gctgagttcg acctgggcct gcccgacgaa aacctcgtgg
      481 gtgtcttgat ccatcgtgag ttcccccgcg tggatcacgg cgtcttcgtc gacgtgcatc
      541 gtgcggcgca agattgcctt gatgcgagcg acgatctcgt cgagactgaa cggcttcgtc
      601 acgtagtcgt cgccgccgac cgtgagcccg gtgatcttgt cttctgtgtc gtctttggcg
      661 gtcaggaaca ggatcggcgc ggtgtaaccg gcgccacgca gtcgtttcgt cacgctgaac
      721 ccgttcatgt cgggcagcat cacgtcgagg atgatgaggt cgggctcttc ttcgagcacc
      781 gccgaaatgg tttgtgcgcc gtttgcgacg gcgcgcacgg cgaagcccgc gaagcgcagg
      841 ctcgttgtta acaagtcacg gatattcggt tcgtcatcga cgatgagaat gcgaggcccg
      901 gcttccatgc ccactattct ctgcgtgagt actggcaact atctgtgtaa cgtgcgtgtg
      961 gtttatggga gctgtgtcag gctgagcggt gcaggggtta tctgcgttct tcctcgtcgc
     1021 gcccagcaag gtgtcgcccg ggtgccggtc gggcggcggc gtcaaggccg accgcgcgca
     1081 cgcagcagcg gcccgcgccc ggatcctgtt cgatgtgccc ctcgcagccg cacgcgtccg
     1141 ccatgccctg caccaggttg aggttgaggc cgcagacgag gccgcgatgg tttcccgcca
     1201 gctcgtcgaa ggggcagttg ccgaagacca cgtcgccgtc ctgctccgta tggggttcgt
     1261 agccgctgac gcgaagcgcc tcgagcaggt cggtgtggcc ggccgccgcc cgggcgcccg
     1321 cctcgcgggc cacctcggcc aacgaggcgt cgatcccctc gccggtcgtg cgcaccctct
     1381 cgacggcgct cgccaagacg tcgccgacga cgtcgtatcg gcgctccggc aggctgagcg
     1441 ttatgacctg cctcgcccgt cggtacagtt tggcgggccg gccggccccc gggccgccct
     1501 tgccgctgcg gcgccgatat tcgacctcga gcaggcccga ttccaccatg cggtcaaggt
     1561 ggaacgccgc cgtgctgcgt ggcattccgg tgagacgtgc cgcgtcgtcg cggctcatcg
     1621 cctcgtcgcg cgtgcggatg agagcataga gtttggtacg agccgggtct cgcaggctcg
     1681 cggccgcctc gaccgcgacg cccgtgcggc cggcgccgct tcgttcgtcg tctgtcatag
     1741 agacagtcta gcctctaaaa tcaagatatt ttgacaatat attgctggga acgtagtctc
     1801 ggttgtgggc acccgccgta ttgttgccat gtcgtgcact gacgcgtgcc ctactcaggg
     1861 gaggaccagc agatgagttc caccatcacg atcgacgaag aagacgcagc gttgcagacc
     1921 atgacggggc aggccccgtc gccacggcag cacgcggagg tgtccgacgg ttcgatgcgt
     1981 caggtgactc ccacgatcga tcgtgtcgcc gcacaggccg cggtcgcaga tctgctgcgg
     2041 gccctgggcc gcgacccgga gaaggagtcg ctggccgaca caccccgccg ggtggcggat
     2101 gcctaccggg agctattgac ccctgtgccg ttcaaggcga cgacgttccc aaatgacgag
     2161 cattacgacg agttggtgct cgtcaaagac atcccgttcc actccctgtg cgagcatcac
     2221 ctgctgccgt tctacggggt ggctcatatc gcctacctgc cggccgaccg tatcgtcggg
     2281 ctctcgaagc tgccgcgcat gctggaatac tgtgcgaggg atcttcaggt gcaggaacgc
     2341 ttgactgtgc aggtcgccga ctggctcgac gcgattctgg aaccccaggc ggtcggcgtc
     2401 gtgctcgagg ctgagcacga atgcatgcga ctgcgcggcg tgaagaccgc cggagcgcgt
     2461 acgcgcacgg tggccgtgcg cggccttctg aagaccgacc gggcgctgcg gcgcgagttc
     2521 ctcgaccgcc tgtaaagcgc ggctggatac ccccgcaagg tccctgtcat ggtcgcctgt
     2581 ggccaggggg cacccgagaa tgctcgagga gacgacgatg ccctgcccac gcggcctcta
     2641 ccgccccgcc atcatgacgc ccgtgcgcac gatcggcgca caccgcttcg acttctcccg
     2701 ccaggtggcc gtcatggcgg ttgtcaatcg cacgcccgac tcgttctatg accgcggcgc
     2761 gaccttcgcg cttgacgcgg cgatccgggc ggccctcgac gccgcccacg agggtgccga
     2821 ctgggtggat atcggcggcg tgcccttctc acccggcgag ccgctgccga ttgaggagga
     2881 ggcggccagg gtcgtgccgg tcgtcgcagg catccgggcg agaagcgacg tcgtgatctc
     2941 gatcgacacc tttcagcccg aggtcgcccg gcgcgcgatc gcggcgggtg ccgacgtcat
     3001 caacgacacc acgggcctcc acgatccggc gatggcggac gtcgtggccg gcagtgcggc
     3061 gagcctggtg atcacgcaca gcctcgcggc gccccgcacc gtgtaccccc ggccgcacta
     3121 cgacgatatc gctggcgagg tcatctcgtt tctacgggag cgggtggatc gtgcgacggc
     3181 gcacggggtg ccggcgaacc gtatcattct cgatccgggc cacgacctca ataagaacac
     3241 ggtgcagagc ctggaggtga ccaggcggtt cgcggagatc gccgacctcg gttttcccac
     3301 gctggcggcc gtgtcgaaca aggacttcat cggcgagacg ttggggttgc cgcgcgggga
     3361 gcgcgtggaa ggctcgctcg cggccgccgt cgcgtgtgtg cttctcgggg cgcgcatcgt
     3421 gcgcatgcat aacgtgcggc agtcggtgcg ggcgatgcgg atggtcgagg caatcctcgg
     3481 ctggcgggag ccggcctacc agaagcacaa catgggcgac gtgaacgagc cggtgcggtg
     3541 acgggcacgg aacctgcggc gatcgaccgg ctctggccgg cgcccgccac agctctcgac
     3601 gatgacaccc tggctgaagg gtacgaggcg ccccggctgc cgtggctgcg cgtcaatttc
     3661 gtggccagcc tggatggctc cgcggcacgc gatgggcgtt ccggcgggtt gtcagacgcg
     3721 gctgaccgcc gggtgttcgc cacgttgcgc aggctttgcg acgtcgtcgt ggtcggcgcg
     3781 ggcacggttc gcgccgaggg atacgggggc atgcgcgtcg acgaggccac ggcggcctgg
     3841 cggagcgaac acgggcgcgc cccgcaaccg cgcctcgcga tcgtcaccaa ccgactccac
     3901 ctgtctgcga cggatgcggc actcgccgag gccgtggcgc gcccgatcgt catcacgtgt
     3961 gcgtccgcac ccgaaacgac gcggcgcgaa ctgagcgagg tggccgacgt gctgatctgc
     4021 ggccaggcgc gcgtcgaacc cgcgcggctc gtcgcggctc tcgctgcgag ggggatgccg
     4081 cgcatgcttt gcgagggcgg ccccaggctc ttcggcgctc tcgccgaggc cggtgtcgtc
     4141 gacgaactct gcctgacgac ggccggcgtt ctggtcgggg gccgcggccc gcgcatcctc
     4201 catgccgatc ggcagctcga cctgtcgatg cgcctcgccc acgttctcgc ggcgggggag
     4261 acgctcctta cgcgctacgt gcgagcaccc tcctcggcgt cgagtgccgc cgcgtcgagg
     4321 atcgtgtagc tgtagccctg ctcggtgagg aaacgctgcc ggttctgggc gaagtcttgg
     4381 tcgacggtgt cgcgggcgac gagggtatag aaacttgcct gcgccccgga ctccttcggg
     4441 cgtagaagcc tgccgagccg ttgcgcctcc tcttggcgcg agccgtaggt tccggagacc
     4501 tgtatcgcga cgctcgcctc cggcaggtcg acggagaagt tcgcgacctt gctgacgacg
     4561 agcaccggcg tgacgccgtc acggaactcc tggtacagcc gctcgcgttc gttcaccggg
     4621 gtcgcgcccg tcagcgcggg cacgcccagc atcgtcgaca gctcgtggag ctggtcgagg
     4681 tattggccga tgacgaggat tcgcgcgccg gcgtgcttgt gcacgagcgt gcgcacgacc
     4741 gccatcttcg ccggcgcgct cgcggcgagg cggaaacgct cgtcgtcgcc ggccgccgcg
     4801 taccccaggc gctcgtcgtc gggcaggtcg acgcgcacct catagcagga ggcgggcgag
     4861 atgaagccct gctcctcgat ctgtcgccac ggggcgtcga accttttcgg gcctatcaag
     4921 ctgaagacgt cgccttcgcg cccgtcttcc cgcacgaggg tcgccgtcag tcccaggcgg
     4981 cggcgggctt gcaggtcggc ggtcagcttg aagacgggcg cgggcagaag gtgcacctcg
     5041 tcgtagacga tgaggcccca gtcgagcgcg tcgagcaacg ccaggtgggt gtattcgccg
     5101 cgccgcctgg tcgtcaagat ctggtacgtg gcgatcgtga cggggcgcac ctctttctct
     5161 tgcccggagt attcgcccac ctgctcgggc agaagcgtcg tgcgtttcac gatctccgcc
     5221 cgccactggc gtgcggcgac ggtgttcgtc acgaggatga gggtcgtcgt gccggtctgg
     5281 gccatcgcgg cgagacccac gacggtcttt cccgccccgc agggcagcac caccacgccg
     5341 ctgccgcccg ccgcgaagct ctccgccgcg ctcgcctgat agtcgcgcac ccgccagccg
     5401 tcttcgacga ggccgatctc gaggggggtt cccggggtgt agccggcggt gtcgtcggcg
     5461 ggccagccga gtttcacgag ttcgcgcttc agctcgccgc gcgcccacgc cgcgacgctg
     5521 aacgatgtcg cgtcacgccg gcctgtgagg agcggagcga ctttcttcgc ccggctcacc
     5581 tcggtcagga ctgcggcgtc gtcggaggtg agcacgagcg tgccgtcctc gtcgcgtgtg
     5641 atggtgagcc ggccgtaccg tgccacggtc tcggtcatgt cgacgacgac gctgcgcggc
     5701 acctcgaact tggagtagcg ctccagcgtc tggagcatcg acgccgcgtc gtgaccggcg
     5761 gcgcgggcgt tccacaaacc gagtcgtgtt atccggtagg tgtgcacgtg ttccggcgca
     5821 cgttccaact cagcgaacac cgccaactcg tgccgggcct cgtccgcgag cggatgcgcc
     5881 acctcgagca gcaccgtgcg gtcgctttgc acgatcaagg ggccgtctga cacaaccctc
     5941 gagtttaccc gccgcgcata tgtgtggctc cgagcgctcc aggcatacgc tgatcggcgg
     6001 ccggataggt ttgatgggcg cccgcccggc gggccgtgct cttggtccgc aagacgcctg
     6061 cggacactct tggcagagaa ggattgtgac gcgtgaacga tcagaccaat gcggggagcg
     6121 aggcgggcga gacacccgaa ccgcttcccg acggggtggc gcgggcgatg ctcgaacgcg
     6181 tggatgcggc ggcggtcttc tacgacgcct cccgcaggcc gcgttggtac aacgcgaggg
     6241 cggcggaatt cgcggccttg gccgggctcg acctcgcgac ggggcgcacc cggcacgtct
     6301 atctggccga tcgagagacg cggctgaccg ggaatgtcga catcctcgac gagacgttgg
     6361 ccgggccggg tcgggggcag ctgtattggc tcggcgaccc gtgcggcacg caggtggccg
     6421 tcgtcagcag agcccatcgg ctcgagacac agggcggggg gttcctgggc tggtcgatcc
     6481 tggcctacga cgtcaccgag acggcgacga tcttcgtcaa ccgagcggag tacttctctg
     6541 ccatcagcca cgaactacgc acgccgctca cgtcgatcct tggctatctc gacctcatca
     6601 tcgacgacgg cggctccggc gacgatgaga tcgaccgcca actacgcatc gtgttgcgcc
     6661 gtgccgaaca gctgtcgaag ctcgtcgccg actatgccga gctttccggc gtcggcaccg
     6721 tgcgtgctca ggagaccgac ctgtgcgccc tcgtcaccga caccgtccgg gcgcgccaag
     6781 agagagccgc ggggcgggag cggccgattc tcaccagggt tcccgaccgg cctctcgtgt
     6841 gccgggtgga cccggggcgg attgcgaagg cgctcgacaa catccttgcg aacgcggtca
     6901 cctacacgcc gcccgaaggc tcgatccgcg tgagcgtcga ggcggactcc accgaggtgc
     6961 gcatcgccgt cgccgacgag ggggccggca tcgacgcagc cgagatgccg aggatcttcg
     7021 accttttcta ccggtcgccg aagcactacc gcaaggtgat ccagggcaac ggcataggcc
     7081 tgcccattgc ccacacgatc gtgaccgcgc atcacggcag gatcaccgtg cattccgtgc
     7141 ccgggcgcgg tgcgacgttc acgatccatt taccctgcgg cgccgtgcgc tgacccgtcg
     7201 ggtgctaggc ggccgcaggg tcccgattcc ggtcaggagt cgggaagatg cccgaggccc
     7261 gtggcagaga cgtctggtgc gcgagtgccg ggggccgggg cggagcgcgt ttcgggctcg
     7321 ggcacccggc cgccgggctc ctgtgcggcg tgggccggct cgccctgtgc cgccggctcc
     7381 ttcggcacgg gcgcgtgttg cagcgccggg ggcacgggag tgcgtaagag aagcaacgtc
     7441 gcgatcgccc cggcgaggaa gagggccgcg ccccaccaga aggcggcgct gtagctcgcg
     7501 atggccgcat gagtgccgag gtcgtgcatg gccgccggcg cgcgcttcag cacgtcggtc
     7561 gcatgggcca gcggcgccga ccatttctcg tgggcggcct gaaccgccgc cgggccgtgt
     7621 gcgcccgccg cggcgatcgc ctctttcgct gcggcgagcg tggccttcgc ggccgcgatc
     7681 ggccgaagga gagcctgctc atgcccgctc agatacgtgc tcgaggcttg gatggcgagg
     7741 gtgttgagga gcgcggtgcc gacggccgcg ccgatctgct gggccgtgtt gacggaggcg
     7801 gaggcggggc cggcctcgtc gtcggagacg tctaacgtgg cgaggttgat gctggtggag
     7861 aacgccatac cgaggcccag tccgatgacg atcaacgggg ccaagagaac gcccgaatag
     7921 gtgctgtcgg ggccgatcct cgtcaagagc gcgaggccgg ccgcggccag ggtcagcccg
     7981 gtcggcagca ggatacgcgg gctgatgcgc cggatgagga tgacgttgct gctgatggag
     8041 gcgagcatca ggcacgcgac catcggcaag aaggcgactc cggtgtgcaa ggccgtgtag
     8101 ccgagcacct gctgcacgta ataggtcacg aacagcaggg caccgaaaag cccggcgccg
     8161 cccaagagca gggccgcgaa ggcggggccc cggtagcggt gtgcgagcac gctgagcggc
     8221 aacagcgggt gccgcacgcg ggtttggagc acgacgaatg cgacgaggag caccgccgcg
     8281 gcaacgagga agccgatcgt cgccgggtcg tcccagttcg ccttgccgtt cgagacggcg
     8341 ttcgagaagc cgtagacgag cgcgaaaagc ccggcggaga cggtgagcac gccgggcacg
     8401 tcgaggtgtg cgccctcgcc gcgggccggg cggtggatca ggatgatcgc gccgcccagc
     8461 gcgaagacgg cgaagacgag gttcacgtag aggcaccagc gccatgagag gtattcggtg
     8521 aggaagccgc ccaaaagcag accgacggcc gcgccgctgc ccaccacggc gccgaacacg
     8581 ccgaaggcgc ggccgcgttc cttcgccgag gtgaacgtgg tggtgagcag cgccagcacc
     8641 gacggcgcca agagcgcggc gaagccgcct tgcacggcgc gcgccgtgac gagcatcgcg
     8701 aagctgccgg cggcgccgcc gacggccgag gccaccgcga aaccgacgag cccgatgatg
     8761 agcgtcgtct tacgcccgag aaggtcggtc aggcgcccgc agaagagcag caagctgccg
     8821 aaggagagcg cgtatgcggt gatgatccac tgccggtcgc ccgtcgtgaa acccaggtcg
     8881 tgctgtgcgg agggcagcgc gatcgtgacg acggtcgtgt cgagcacgat catcagttgg
     8941 gcgagcgcga gcaccgcgag cacccacatg cggtgcgcct gcccgcccgg caggttcgac
     9001 gtgtcgtcag cgagtctgtc gcgggcttcc gctcggcgga tatctaccac gggtagacct
     9061 tacttctctg tgttagctat cacccgaacg tgccggaccc gacttccctc aaacgtgctc
     9121 gtcacgcctc cggtgacggg cgaggtaggt gtcggcgagg tgggtcaagg cgggcagggc
     9181 cgcggcgatc gccgcccgct ccgcggcgtc taagtggtcg aggctgtcgc tgagctccgt
     9241 cgtgccctcc tgccgcagct tgccgagacg ttccgtgccg tcgggcgaaa gccccacgag
     9301 agaggcgcgc tggtcgtcgg ggtcgggctg gcgacggacc cacccgccga cctgcaaggc
     9361 ctcgaggagg cgggacatgc tggcggcgct gatgccgagg ccgcgcgcca gctggcccgg
     9421 gcgaacaggg cccgtcttct ccagcgagac aagggcagtc agacgggtcg gagtgaggcc
     9481 gctctgcgcc gcggggccgc gcaggcagta gctgagcctg ccgaccgagg tgcgcagcat
     9541 cccggcgagg tccgtccctg tcggaggcct cgaggggtcg ccggcacggc cggcctgggc
     9601 ggggtttgtc gcggtggcgg ggctcatcgc ccgcctcgcg gggtgagaat gccgtcagac
     9661 gggtggtctt cgtgcccggc cgggccgtac gacgtgtctt tccccgccgc cgaatcgtcg
     9721 gggtagacgt actttccccc gcgcagcgcc gacgccgcgg ccgccacgag gcaggccgcc
     9781 gccgcgaatc cgaaggcgac ggcgaggccc gccgagaacg gccccgagat gagggcgggg
     9841 aagaactgcc gccccgtgag gtaggccgcg tgcccggcgg agagatgcgc cagaacgtgc
     9901 gggccgagaa gggtctgtac cgggttatag ccgagcagcg aggcgaagag caccgcgacg
     9961 ggcggcagcg cggcgacctg cgcggcggcg gccgccggca cgccttggcc ggtgagcccg
    10021 gtgacgagcg tgtggggcag gcttgcggcg agcccgacga tcatcagcgt gaagaagatg
    10081 ccgatgctca acaccatggc cgaattctgg aacgtgtagc tcatgccgga gccgacgccg
    10141 cgctggtggg ccggcaggct gttcatgatg ccggtgcggt tcggggccga gaagagcccc
    10201 atgccgatcc cgttgacgag cagggcgagg gcgaactgcc agtaggagaa gttcaccggc
    10261 atgaggtaga gccagccgaa gctcgcggcg gcgatgatca tgccgccggt ggcgaagcct
    10321 cgtgcgccga acttgtcgga aagccaaccg gaggtcgggc cggcgatcaa gaagccgacc
    10381 gtcatcggca gcatgtagat gccagcccat aggggagtgg agacgaagct gaagccgtgc
    10441 cggggcagcc agatgccctg cagccagatg atcaagatga acatcaggcc gccacggccc
    10501 agcgcgctca acaggccggc gaagtttccc gctgtgaacg cgcggatgcg gaacaggtcg
    10561 aggcggaaca tcggctgggc gacccgggtc tccaggtagc agaacccgac gaggatcacg
    10621 gcgccaccga ccagcgtggt gaggacgaac gggctggtcc agcccatcgt gtggccgccg
    10681 tagggctgga tcccgtaggt gatgcccacg agaagggcga tgaggccgac cgcgaaggtg
    10741 atgttgcccc accaatccat gcgggcga
//
LOCUS       DASTNL010000152         6340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_847381, whole genome shotgun sequence.
ACCESSION   DASTNL010000152 DASTNL010000000
VERSION     DASTNL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847381"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFQ96_04755"
     CDS             <1..184
                     /locus_tag="VFQ96_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257132.1"
                     /translation="RNAVRGENAIVTEPPMTATTTAAEARTTARRRLAWRPNRRASPR
                     SARRLLRRAVRNASRA"
     gene            complement(428..1786)
                     /gene="murC"
                     /locus_tag="VFQ96_04760"
     CDS             complement(428..1786)
                     /gene="murC"
                     /locus_tag="VFQ96_04760"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU0257133.1"
                     /translation="MTAVHFVGVGGSGMSGIARLFLQAGIAVTGSDRSESANVTALRG
                     LGAQIAIGHDAAHVGEVDAVVVTGALWKDNPEYLAALERGIPVLHRSQALKWLIGRRR
                     LISVAGAHGKTTSTGMIVTALLALDEDPSFVNGGVIEALGTSSAHGSGELFVAEADES
                     DGSFLLYDTAVALITNVDPDHLDHYGTLERFEAAFVTFASAARERVVLSADDPGARDI
                     AARLVDQRVVTFGESPQADARLSDVTATGPVSFTIHWRGQSYAVALRIPGRQNAVNAT
                     GAFATLAAMGFDPHKIIEALQTFRGTRRRFELRGTVGGVSVYDDYAHHPTEIQAALTA
                     ARSVLGGGRIIAVHQPHLYSRTKIFANEFAKTLERYADQTIVLDVCGAREDPVPGVTG
                     ALVSERFHDPSKVAYIADWQKAADYTASLAREGDFIITLGCGDVYRIIPQLLESLHAA
                     RG"
     gene            complement(1822..2925)
                     /locus_tag="VFQ96_04765"
     CDS             complement(1822..2925)
                     /locus_tag="VFQ96_04765"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU0257134.1"
                     /translation="MSGYLLAGGGTAGHVNPLLASADRIRERDPEARILVLGTAEGLE
                     SRLVPGHGYELAVIPKLPFPRRPGGYALRFPFALYGAVRRVRRMLRERRIDVVVGFGG
                     YAAAPAYLASSGARVPIVVHEANARAGWANRLGARRAARVGVAFEGTGLPGARLIGMP
                     LRHEIEELDRGASRAAAARSFGLDPARPILLVTGGSLGARSINTTLVRRAEQITAAGW
                     QVLHIQGGREEIADPHVDGYRLIGYCDRMADALAAADLALARAGAGTVSEFGALGIPA
                     IYVPFPIGNGEQRLNAAGIVAAGGGLLVDDADLTPEWLDRELLPLIADPARVREMARR
                     AASQGTRDGTARLVDLIMEVRPHKAITQEGGNP"
     gene            complement(2922..4169)
                     /locus_tag="VFQ96_04770"
     CDS             complement(2922..4169)
                     /locus_tag="VFQ96_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU0257135.1"
                     /translation="MSARMPAPAVVSRAVGRAPRITRLTLGGERRPMLPRYAFLLGVT
                     LFMTVFGLIMVLSSSSVTSHDGGGSLLGQFLKQGSFALVGVPLMLIVSRIPTRTIRRA
                     AWPLMLGAFALQLLVVATPLGHGAFGNRNWLQLGSLPAIQPSEAIKLAMVIWLGMFFV
                     KRRGRMGEWKYFLLPVVLVTGFGMFLVLLGDDLGTVMIMALTLIGALFFGGVRLRHIG
                     AVLAAGALVVLLFVVTSANRLSRIGSFLDPGTAGAASRWQTENGNFALASGGIFGVGL
                     GNSHSKWAWLPSADTDFIFAIIGEELGMIGAILVIILFALLIVAFLRIYHATPDPAGR
                     VTTAAVMVWLIGQGFVNIAVVLGALPTLGVPLPLFSSGGTAMISSLLAIGIVLSYARE
                     IPKGAADRPPARAGASHAGRLAR"
     gene            complement(4166..5692)
                     /gene="murD"
                     /locus_tag="VFQ96_04775"
     CDS             complement(4166..5692)
                     /gene="murD"
                     /locus_tag="VFQ96_04775"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU0257136.1"
                     /translation="MADTLTTTAPNTHDPLDRLVSWHASWRGLRTLVLGLGVTGFAAA
                     DTLAELGADVLVAAEHAEAGRRQMLDAIGARLLETELRAVPGEVRRFAPEVIIASPGF
                     HPDHALLSWAGHAGIPVWGDIELAWRLRDKTGRPADWLAVTGTDGKTTTVQLSAEILR
                     QAGLRVAPCGNIGVPVLDAIRDPQGFDVLVVELSSYQLHWTNRNAAGALSPYASVCLN
                     IAPDHLDWHGSFENYRAAKARVYENTRVACVYNKADEATVAMVREAEVVAGARAIGFG
                     RGAPGPGDLGVVGGVLCDRAFLDDRAERALELTTLDELAGRGLAAPHLVEDVLAAAAL
                     TRSYGVAPEVIHEALTHFTVDQHRVETVAVRGGTRWVDDSKATNPHAAAAALTAFPRI
                     VWLVGGAVKGVDVGPLVEKFAPRLRAAVILGADRAAFRAAFRRHAPQLPVFEIQAADT
                     EEIMRQAVEYAAGKAGPGDTVLLAPAAASTDQFANYAQRGSMFAAAVRALPAAERPAE
                     "
     gene            complement(5677..>6340)
                     /gene="mraY"
                     /locus_tag="VFQ96_04780"
     CDS             complement(5677..>6340)
                     /gene="mraY"
                     /locus_tag="VFQ96_04780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU0257137.1"
                     /translation="DLPVNFMALSALGAAGVVVAFVLYFAWINLFATATTNGVNITDG
                     LDGLATGVSILVLSAYVTMGFWQSIQSCHSPRLDPSNVAKCYEIRDPIDLAIIATTLV
                     GALVGFLWWNTSPAQIFLGDTGSLAIGGAIAALAILSHTEILLFLIGGLFVIEAGSVI
                     VQRAYFKVTHGRRIFLMSPIHHHFELKGWAEVTVVVRFWLIAGFFVACGIGLFYIEWL
                     TH"
BASE COUNT          995 a         2164 c         2298 g          883 t
ORIGIN      
        1 gcgcaacgcc gtcagagggg agaacgccat cgtcaccgag ccgccgatga ccgcgaccac
       61 gacggctgcc gaggcaagga cgacggcgcg gcgtcgcctc gcctggcgcc cgaatcgacg
      121 tgcctcacct cgctcggccc ggcgtctgtt gcgtcgcgcc gtgcgcaacg cgtcgcgggc
      181 ctgacgcagc cgttcgcccg gcgccgttgc cccccgatgc gcgtctgcag tgctcgggcg
      241 gtccccgtgg gcggtgcggt cgggtgaggg agagcggtcg tgcgcacgcc cgcgcgcgcc
      301 cggtgcctcg tgcggggcct cgcccggcga ggcggggccc ggtcgtgcgc cgcccgggcc
      361 cgggtggccg ggcccggtgc ctcgtggttc cgggctgcgc ggcccggcgg gtcgtctcat
      421 ggcctcgtca tccgcgtgcc gcgtgcagag actcgagaag ctgggggatg atccggtaga
      481 cgtcgccgca gccgagcgtg atgatgaaat cgccctcccg ggcgaggctc gcagtgtagt
      541 ctgcggcctt ctgccaatcg gcgatgtagg caaccttcga cgggtcgtgg aaacgctctg
      601 agacgagcgc gcccgtgaca cccggaaccg ggtcttcgcg ggccccgcac acgtcgagca
      661 cgatcgtctg gtcggcgtaa cgctcgaggg tctttgcgaa ctcgttcgcg aagatcttcg
      721 tcctgctgta gaggtgtggc tgatggacgg cgatgatccg cccgcctccc aagacggatc
      781 gggcagctgt cagcgccgcc tggatctccg tcgggtggtg cgcgtagtcg tcgtatacgc
      841 tcaccccgcc gaccgtgccg cgcagctcga atctgcgcct cgtcccccgg aaggtctgga
      901 gggcctcgat gatcttgtgc gggtcgaagc ccatcgccgc gagcgtcgcg aaggcgcccg
      961 tcgcattcac cgcgttctgc cggccgggga tgcgcagggc gacggcatac gactgccctc
     1021 gccagtgaat cgtgaacgag accgggcccg tcgccgtgac gtcggagagc cgggcgtctg
     1081 cctgcgggct ctcgccgaac gtcacgaccc tctggtcgac caggcgggcg gcgatgtcgc
     1141 gggcgcccgg gtcgtcggcg gagagcacga cccgttcgcg ggcggcggag gcgaacgtca
     1201 cgaaggcggc ctcgaatctc tccagcgtgc cgtaatggtc aagatggtcg gggtcgacgt
     1261 tcgtgatgag cgccaccgcg gtgtcgtaga gcaggaagct gccgtcggac tcgtcggcct
     1321 cggcgacgaa gagctccccg ctgccgtgcg cgctgctcgt gccgagcgcc tcgattaccc
     1381 cgccgttcac gaagctcggg tcttcgtcca gggcgagaag cgccgtgacg atcatgccgg
     1441 tcgaggtcgt tttgccgtgc gcccccgcga cggagatgag gcgccgccgt ccgatcagcc
     1501 acttcaacgc ctgcgagcgg tgcagcaccg ggatgcctcg ctcgagtgcc gcgagatact
     1561 cggggttgtc cttccacagg gcgccggtga cgacgaccgc gtcgacctcg cccacgtggg
     1621 cggcgtcgtg cccgatggcg atctgcgctc cgagaccgcg caacgccgtc acgttcgcgg
     1681 actcgctgcg atccgacccg gtcaccgcga tcccggcctg caggaacagc cgggcgatac
     1741 cgctcatccc cgagccgccg acgccgacga aatgcaccgc cgtcatcgtc ggcgggacca
     1801 ccacgttcgg gtcgagatcg atcacgggtt cccgccttcc tgggttatgg ctttgtgcgg
     1861 ccgcacctcc atgatgaggt cgacgaggcg tgccgtgccg tctcgggtgc cctgactagc
     1921 cgcccggcgc gccatctctc gcactcttgc cgggtcggcg atgagcggca ggagctcgcg
     1981 atccagccat tccggggtga ggtccgcgtc gtcgacgagg aggccgccgc cggccgcgac
     2041 gatgccggcc gcgttgaggc gttgctcccc gttgccgatc gggaacggga cgtagatggc
     2101 agggatgccc agggcgccga attccgagac ggttccggcg ccggcccggg cgagtgcgag
     2161 gtcggccgcc gccaacgcgt cagccatgcg gtcgcagtag ccgatcagcc gatagccgtc
     2221 gacatgcggg tcggcgatct cttcccgccc gccctggatg tgcaaaacct gccaacccgc
     2281 cgcggtgatc tgttcggccc gcctgacgag cgtggtgttg atgcttcgtg cgccgaggga
     2341 tcccccggtg acgagcagaa tggggcgggc cggatcgagc ccgaaggagc gtgccgcagc
     2401 cgcgcgggag gctccccggt cgagttcttc gatctcgtgc cgcaacggca tgccgatgag
     2461 ccgcgcaccg ggcaggcctg tgccctcgaa ggcgacaccg accctggcgg cgcgccgggc
     2521 gccgagccgg ttcgcccacc ccgcgcgggc gttcgcctca tgcaccacga tcggcacgcg
     2581 tgcgccggag gaggcgaggt aggcgggcgc cgccgcgtat ccgccgaacc ccacgacgac
     2641 gtcgatgcgg cgctcgcgca gcatccggcg cacccggcgc accgcgccgt agagggcgaa
     2701 cgggaagcgc agggcgtagc cgccggggcg cctcgggaac gggagtttcg ggatcacggc
     2761 gagctcgtag ccgtgcccgg gcacgagcct cgactcgaga ccttcggcgg tgccgaggac
     2821 gaggatgcgt gcctcgggat cccgctcgcg aatgcggtcg gcgcttgcca ggagtgggtt
     2881 cacgtgcccc gcagtgccgc cgccggcgag gaggtatccg ctcaccgggc aagccgcccg
     2941 gcgtggctgg cgcccgcccg ggccggcggc cggtcggcgg cccccttcgg gatctcgcgt
     3001 gcgtacgaca acacgatgcc gatggcgagg agggagctga tcatggcggt gccgccggag
     3061 gagaagagcg gcaagggaac gcccagcgtc ggcaatgcgc ccaagacgac ggcgatgttc
     3121 acgaagccct gcccgatcag ccacaccatg accgcggccg tcgtgacccg tcccgccggg
     3181 tcgggggtgg cgtgatagat gcgcaggaag gcgacgatga gcaaagcgaa aaggatgatg
     3241 acgaggatcg cgccgatcat gccgagttcc tcgccaatga tggcgaagat gaaatcggtg
     3301 tcggccgagg gcagccacgc ccacttcgag tgcgagttgc cgaggccgac gccgaagatg
     3361 ccgcccgagg cgagggcgaa gttgccgttc tcggtctgcc agcgtgacgc ggcgccggcc
     3421 gtgcccgggt cgaggaacga accgatcctg ctcaagcggt tcgcgctcgt cacgacgaac
     3481 agcagcacga cgagtgctcc ggccgcgagc acagcgccga tatggcgcag ccgcaccccg
     3541 ccgaagaaca gcgctccgat cagggtcaga gccatgatca tgacggtgcc gaggtcgtcg
     3601 ccgagcagca ccaggaacat gccgaacccg gtcacgagga cgacggggag aagaaagtat
     3661 ttccactcgc ccatgcggcc ccgtcgcttc acgaagaaca tgcccagcca gatcaccatg
     3721 gccagcttga tcgcctcgga gggctggatg gccggcagcg agccgagctg cagccagttc
     3781 cggttgccga aggcgccgtg gccgagcggc gtcgccacga cgagcagctg cagcgcgaac
     3841 gcgcccagca tgagcggcca ggccgcgcgt cggatcgtgc gggtcgggat acgagagacg
     3901 atcagcatga gcggcacgcc gacgagggcg aaactgcctt gcttcagaaa ctgccccaga
     3961 aggctgccgc caccgtcatg cgaggtgacc gacgaggagg agagcaccat gatgagcccg
     4021 aagaccgtca tgaagagtgt gacgccgagg aggaaggcgt agcgcggcag catcggccga
     4081 cgctcgccgc ccagcgtcaa gcgggtgatc ctgggggcgc ggccgacggc acgcgagacg
     4141 acggcgggcg ccggcatgcg cgcgctcatt ccgcgggcct ttcggcggct gggagcgcgc
     4201 gcaccgcggc agcgaacatg ctgccccgtt gggcgtagtt cgcgaactgg tcggtcgagg
     4261 ccgcggcggg cgcgagcagc acggtgtcgc ccgggccggc cttgcccgcc gcatactcca
     4321 ccgcctgacg catgatctct tcagtgtcgg ccgcctgaat ctcgaagacg ggcagctgtg
     4381 gcgcgtgtcg ccggaatgcc gcacggaacg ccgcacgatc cgcgccgaga atgaccgcgg
     4441 cccgcaggcg cggcgcgaac ttctcgacga gcggcccgac atcgacgccc ttcacagcgc
     4501 ccccgacgag ccagacgatg cgcgggaatg cggtgagggc ggccgcggct gcgtgcgggt
     4561 tcgtcgcctt cgaatcgtcg acccagcggg tgccgccccg cacggcgacg gtctcgacgc
     4621 ggtgctggtc caccgtgaaa tgcgtcagcg cctcgtggat gacctcgggt gcgaccccgt
     4681 acgaccgggt caaggcggcg gcggccagca cgtcttcgac gaggtgcggc gcggcgaggc
     4741 cccgcccggc cagctcgtcg agcgtcgtca actcgagggc gcgttccgca cggtcgtcga
     4801 ggaaggcgcg gtcgcagagc accccgccga cgacgccgag gtcgcctggg ccgggggcgc
     4861 cccggccgaa gccgatggcg cgggcgcccg ccaccacctc cgcctcacgg accatcgcca
     4921 cggtcgcctc gtcggccttg ttgtagacgc aggccacgcg ggtgttctcg tagacacgtg
     4981 ccttcgccgc ccggtagttc tcgaacgagc cgtgccagtc aaggtggtcg ggcgcgatgt
     5041 tcaggcagac gctggcgtaa ggcgagagcg cacccgcggc attcctgttc gtccagtgca
     5101 gctggtagct ggagagctcg accacgagga cgtcgaagcc ttgcgggtcg cggatcgcgt
     5161 cgagcaccgg caccccgatg ttgccgcacg gcgcgacgcg cagcccggcc tgccgcagga
     5221 tctcggcgct caactgcacc gtcgtcgtct tgccgtcggt gccggtcacc gcgagccagt
     5281 cggcgggccg ccccgtcttg tcgcgcagcc gccaggccag ctcgatgtcg ccccagacgg
     5341 ggatcccagc atgcccggcc caggacaaga gggcatggtc ggggtggaag ccgggcgagg
     5401 cgatgatgac ctccggggcg aagcgccgga cctcccccgg cacggcgcgc agctcggtct
     5461 cgagaaggcg cgcgccgatc gcatcgagca tctgtctgcg gcccgcctcc gcgtgctcgg
     5521 cggcgaccag cacgtcggcg ccgagctccg ccagtgtgtc ggccgccgcg aacccggtga
     5581 caccgaggcc gaggacgagg gtgcgaaggc cccgccacga ggcgtgccag ctgacgagcc
     5641 ggtcgagcgg gtcatgcgta ttcggcgccg tggtcgtcaa tgtgtcagcc attcgatgta
     5701 gaagaggccg atgccgcagg ccacgaagaa gccggcgatc agccagaaac gcaccaccac
     5761 cgtcacctcg gcccagccct tcagctcgaa gtggtgatgg atggggctca tcaagaagat
     5821 gcgtcggccg tgtgtcacct tgaagtaggc gcgttgcacg atgacggagc ccgcctcgat
     5881 cacgaacagg ccgccgatga ggaagagcag gatctccgtg tggctcaaga tggcgagggc
     5941 cgcgatcgcg ccgccgatcg cgagcgagcc cgtgtcgccg aggaagatct gtgccggcga
     6001 ggtgttccac cacaagaatc cgacgagggc gccgaccagc gtcgtcgcga tgatggcgag
     6061 gtcgatggga tcgcggatct catagcattt cgccacgttc gacgggtcga gtctcgggct
     6121 gtgacacgac tggatcgact gccagaagcc catcgtcacg tatgcgctca agacgaggat
     6181 cgacacgccg gtggccaggc cgtcgagccc gtcggtgatg ttgactccgt tggtcgtcgc
     6241 cgtcgcgaag aggttgatcc acgcgaagta gagcacgaag gcgacgacga cgcccgcggc
     6301 gccgagagcg gagagcgcca tgaagttgac cggcaggtcg
//
LOCUS       DASTNL010000153         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_107032, whole genome shotgun sequence.
ACCESSION   DASTNL010000153 DASTNL010000000
VERSION     DASTNL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107032"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFQ96_04785"
     CDS             complement(<1..1383)
                     /locus_tag="VFQ96_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0257138.1"
                     /translation="MDTSGRRHADVIIVGGGSAGCVLANRLSEDPARRVLLIEAGHRV
                     IDPDVARPERWVSLPGRDYDWAFETTPQAGLDGRVMPWPRGRGLGGSSNLHAMAHMRG
                     DAADFARWARGAAGPAESTAGGQDPRLSRWSWHALLPFFRATERFTSPTPDSRPDLHG
                     STGPMPVVVPGPGRLSPLVADYLAAWESLGLARIEDHNHGSMVGATANALTIDGGRRV
                     TAADAYLFPVRDRPNLEVLEDAVVHRVMLHRGAVRGVVWSRSGGDGATRRAESDTVIL
                     AAGAVADPLVLLRSGVGDPAALAAAGVPVSHPLPGVGRGLHDHLLGAGNLYRSRRAVP
                     PSLLQLSEAMTYLPMGGPGAAGRPELVVGCVVGPSVATTFVPDPRWAGHSYTLLFGLT
                     HPTSRGTIRITGPDVGDPALIDPAYLTTRHDRSLFRRALEMARLVGHSARLAAWRDEE
                     ILPGPGRTDDE"
     gene            complement(1388..2122)
                     /locus_tag="VFQ96_04790"
     CDS             complement(1388..2122)
                     /locus_tag="VFQ96_04790"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0257139.1"
                     /translation="MTNSTDRVAIVTGAAQGIGAAIARELANKGHRVVVADINLAGAQ
                     KVADDIDGHAVRVDVSDPEQVRALTAEVMSTFGRIDILVNNAALVPFVPWEKITFEEW
                     RRVMSVNLDGLFLMIRAVGDEMAKAGYGRIVNIASNTFVAGTPNCAHYVATKGASIGL
                     VRALAGEVGKHGITVNAVAPGLTETEGVLAGPHKEGFGYVQAAQAFDRRGLPEDIAPA
                     VAFLASEEAGWITGQTLVVDGGHTRN"
     gene            complement(2215..>2777)
                     /locus_tag="VFQ96_04795"
     CDS             complement(2215..>2777)
                     /locus_tag="VFQ96_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU0257140.1"
                     /translation="PEQSAEQTIPAQLALCGYRPEDVDIVINSHFHFDHVGGNKYFTG
                     ARVLVHKQELRQAKVPESFERLGYSDLSFDFPGVRYEPIAGDYEIVPGVTLFETPGHT
                     VGHYSMLVEMQGEKSMLFAGDAAYTDRTLHDEIIGGFHNDPTASLESIRRLKYLSRTK
                     GADIYPSHEMEPFLTWKHAPQYYGGN"
BASE COUNT          410 a          923 c         1016 g          428 t
ORIGIN      
        1 ctcgtcgtcg gtccggccgg ggcccggcag gatctcctcg tcgcgccatg cggcgagcct
       61 ggcgctgtgg ccgacgagtc tcgccatctc gagtgcgcgg cggaacagag agcggtcgtg
      121 gcgggtcgtg aggtacgccg ggtcgatgag tgccgggtcg ccgacatcgg gcccggtgat
      181 acggatcgtg ccacggctcg tcgggtgcgt gaggccgaac agaagggtgt acgaatggcc
      241 cgcccaacgc ggatcgggaa cgaatgtcgt agcgacgctc gggccgacga cgcagccgac
      301 gacgagttct ggccggcctg cggctccggg gccgcccatc ggcagatacg tcatcgcctc
      361 ggagagctgg aggaggctcg gcggcacggc gcggcgcgaa cggtagaggt ttccggcgcc
      421 gagcagatgg tcgtgcagac cccgtccgac ccccggcagc gggtgtgaca ccggcacccc
      481 tgccgcggcg agggcggccg ggtcgccgac cccggagcgc aggagcacga gcgggtcggc
      541 cacggcgccc gcggcaagga tgacagtgtc ggattccgct cgcctcgtcg cgccgtcacc
      601 gcccgaacga ctccacacga cgccgcgcac ggcgccgcgg tgcagcatca cccggtggac
      661 gacggcgtct tcgagcactt cgaggttcgg gcggtcgcga acggggaaga ggtaggcgtc
      721 ggcggcggtg acacgtcggc ccccgtcgat cgtgagcgcg ttcgccgtgg cgcccaccat
      781 cgacccatgg ttgtggtcct cgatgcgggc cagccccaga gactcccagg cggcgaggta
      841 atcggccacc aacgggctga ggcggcccgg cccgggcacg acgaccggca tggggcccgt
      901 gctgccgtgc aaatcggggc ggctgtccgg ggtggggctc gtgaagcgtt cggtcgcgcg
      961 aaagaacgga aggagcgcgt gccacgacca gcgtgacagc cgcggatcct ggccgcccgc
     1021 ggtgctctcc gcagggcctg ccgcgccgcg cgcccagcgc gcgaaatcgg cggcgtcgcc
     1081 gcgcatatgg gccatcgcgt gcaggttgct cgaaccgccg aggccccggc cgcgcggcca
     1141 gggcatgacg cgcccgtcga gcccggcctg cggggtcgtc tcgaaggccc agtcgtagtc
     1201 acggccgggc agcgacaccc accgctccgg tcgtgccacg tcggggtcga tgacgcggtg
     1261 gccggcctcg ataaggagga cccggcgcgc cggatcctcg ctcaaccggt ttgcgagcac
     1321 acagcccgca gagccgccgc ccacaatgat gacgtcggcg tgccgccggc cggaagtatc
     1381 cacggcgtca gttgcgagtg tggccgccgt cgaccacgag cgtctggccg gtgatccaac
     1441 cggcctcctc cgaggcgagg aaggcgacgg cgggcgcgat gtcttcgggc aggccgcgcc
     1501 ggtcgaaggc ctgcgcggcc tgcacgtagc cgaagccttc cttatggggc ccggcgagca
     1561 ccccctccgt ctcggtgagg ccgggggcga cggcgttcac ggtgatgccg tgcttgccga
     1621 cctcgcccgc caaggcgcgt acgagcccga tcgaggcacc cttggtggcc acatagtgtg
     1681 cgcagttcgg ggtgcctgcg acgaaggtgt tggaggcgat gttcacgatg cggccgtagc
     1741 cggccttcgc catctcgtca ccgacggccc ggatcatgag aaagagcccg tcgagattca
     1801 ccgacatgac tctgcgccac tcctcgaagg tgatcttctc ccacggcacg aacggcacga
     1861 gggcggcgtt gttgacgagg atgtcgatgc ggccgaacgt ggacatcacc tcggcggtca
     1921 acgcccgcac ctgctcgggg tcgctgacgt ccacgcgcac cgcgtgcccg tcgatatcgt
     1981 cggcgacctt ctgggcgccg gccaggttga tgtcggcgac caccactcgg tggcccttgt
     2041 tcgcaagttc ccgggcgatg gccgcgccga taccctgggc cgcacccgtg acgatcgcca
     2101 cccggtccgt tgaattcgtc atgtcttcgg ctccgttctg gtcggggtct cgcgctcatc
     2161 ggctgacccg gtggtggtcg ggtaggcggg cctcgcccgg ctcagcgttg cgggtcagtt
     2221 gccgccgtag tactggggag cgtgtttcca ggtgaggaac ggctccatct cgtgcgacgg
     2281 gtagatgtcg gcgcccttcg tgcgggaaag gtacttcagg cggcggatgg actccagcga
     2341 cgcggtcggg tcgttgtgga atccaccgat gatctcgtcg tgaagcgtgc ggtcggtgta
     2401 ggcggcgtcg ccggcgaaga gcatcgactt ctcgccctgc atctcgacca gcatcgaata
     2461 gtggcccacg gtgtggccgg gcgtctcgaa caaggtgaca cccggcacga tctcgtagtc
     2521 gccggcgatc ggctcgtagc gcacgcccgg aaagtcgaac gagaggtcgg agtagccgag
     2581 ccgctcgaac gattccggca ccttcgcctg ccggagctct tgcttatgga cgagaacccg
     2641 cgcgcctgtg aagtacttgt tgccgccgac gtgatcgaag tgaaagtgtg agttgatgac
     2701 gatgtcgacg tcttcgggcc ggtagccgca cagtgccagc tgggcgggga tggtctgctc
     2761 ggcgctctgt tctggca
//
LOCUS       DASTNL010000154         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_477760, whole genome shotgun sequence.
ACCESSION   DASTNL010000154 DASTNL010000000
VERSION     DASTNL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477760"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VFQ96_04800"
     CDS             <1..1167
                     /locus_tag="VFQ96_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0257141.1"
                     /translation="ENRAVTWQAWHRAIGQSVVDRQAIDARAPKPSDAAQLLFTSGTS
                     GEPKGVIHRHESLSRAAWMEVEQLPLNTTDSIYIPSPLAHQTGFLYGMWLSWVLGVTE
                     IVQAVWDPQRALDVLQTWEGTFVQAATPFLTDLVRAVEAGAPAPRALRIFVATGAAVP
                     RALAERATRVLGAAVCGAFGTTETGLGALSSPADPAAKAWGTDGKAMPGAKLRIVDDE
                     GRPVPSGVEGNFELTSPTVFDGYLDRPDLTAEVFTDDGWYRTGDLATIDDEGYLRITG
                     RVRDVINRGGEKIPVSEIEQLLYEHPAIDDVAIVAMPDERLGERACAFVTLADGARLS
                     FEGMQTFLDQKNISKYYWPERLEVIDEIPRNVVGKIQKFKLRERAAGLTSQRTK"
     gene            1212..2384
                     /locus_tag="VFQ96_04805"
     CDS             1212..2384
                     /locus_tag="VFQ96_04805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0257142.1"
                     /translation="MATTTNTEYEALVADVTAWVEGPGEEWARRVESTGTVPDALFSE
                     LRERGYLSMAAPVELGGRGLSFSQWMGLMEVFSRSHASIRMLVHVVNGTWRAMVPYAN
                     AQQVEKYIKPSILGEKLIAFTLTEPGNGTGADISCSVRREGDTYYLSGQKHLITFGLK
                     CDWWLLFARLEGTTGGDQLVGLLVDRGAAGVTATDMGETMGVHGTDHALLTFENTPVP
                     VAQRLGEEGDGLEMALGGFLTPSRVSVAMSCVGLAERAQQLAVDYAATRVTFGKPIAK
                     RQAIAFMIAENQADIEAAKQLVLHAARRWDAGDPEASALSSMAKIVAVDMLGRVTDKA
                     LQVHGGIGYWKSSAIERVYRDARAQRFEEGTNEIQKTVVARDIFRAREAARSGGAR"
     gene            2381..3229
                     /locus_tag="VFQ96_04810"
     CDS             2381..3229
                     /locus_tag="VFQ96_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0257143.1"
                     /translation="MSDAAGPRQGEGRYAGRIALITGSSRGIGRTLALGLAAQGATVV
                     VNYRKNADLAAEVVAEAKRLGGDGIAVGADVEQPDEITRMFDTVADTYGRLDFFVNNA
                     AAAAFKRILDLKPHHLDRSYATNVRPFVLGAQQAVTLMDRGGRIVAVSSYGSIRAFPT
                     YAALGSYKAAIESFIRYMAVEFAPYGINVNGVNGGLIQSDSLDFFYSVPGMSPMQKVL
                     DTVPLGRPGTVQDMADAIEFLLSPHSAYITGQTLVVDGGMTVAAAPFWRDTTSPLGIP
                     ERPTRG"
     gene            3235..>3736
                     /gene="fadH"
                     /locus_tag="VFQ96_04815"
     CDS             3235..>3736
                     /gene="fadH"
                     /locus_tag="VFQ96_04815"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEU0257144.1"
                     /translation="MPETTFPRLREPVTIGSLRLPHRVIMGSMHLNRERGPADALAAF
                     YVERVRGGAALIVTGGAAVSRVGAGSSAYSLINEAPDQETWQVVVDAVHQAGGTIALQ
                     LFHAGRYAGTEAFGLTPVAPSAVYSRFSGCKPEALTEEGVRRTLDDFAAGAKVAAGLG
                     FDAIEVM"
BASE COUNT          611 a         1337 c         1250 g          538 t
ORIGIN      
        1 gagaaccgtg ccgtcacgtg gcaggcctgg catcgagcga tcggccagag cgtcgtcgac
       61 cgccaggcga tcgacgcccg cgcgccgaag ccgtcggatg cggcgcagct gctcttcacc
      121 tccggcacct ccggcgagcc gaagggcgtc atccaccgcc acgagagcct cagccgcgcc
      181 gcctggatgg aggtggagca gctgccgctg aacacgaccg actccatcta cataccctcc
      241 ccgctcgccc accagaccgg cttcttgtac ggcatgtggc tctcctgggt gctcggcgtc
      301 accgagatcg tgcaggccgt gtgggatccg cagcgcgcgc tcgacgtgct ccagacgtgg
      361 gagggcacct tcgtgcaggc cgccacgccg ttcctgaccg acctcgtgcg tgccgtcgag
      421 gccggcgccc cggcgccccg tgcgctgcgc atcttcgtcg ccacgggcgc cgccgtgccg
      481 cgcgccctgg cggaacgcgc cacccgcgtg ctcggcgcgg ccgtgtgtgg cgcgttcggc
      541 acgaccgaga cgggcctcgg cgccctctcg agcccggccg acccggcggc gaaggcgtgg
      601 ggcaccgacg gcaaggcgat gcccggcgcc aagctgcgca tcgtcgacga cgaaggcagg
      661 cccgtgccct ccggcgtgga aggcaacttc gagctgacaa gccccaccgt cttcgacggc
      721 tacctcgacc ggcccgacct cacggcagag gtcttcaccg acgacggctg gtaccgcacc
      781 ggcgacctcg cgacgatcga cgacgagggg tacctgcgca tcacgggccg cgtgcgcgac
      841 gtcatcaacc gcggcggcga gaagatcccg gtctccgaga tcgagcagct tctctatgag
      901 caccccgcga tcgacgacgt ggccatcgtc gccatgcccg acgaacggct tggcgaacgg
      961 gcctgtgcgt tcgtcacgct cgccgacggc gcgcggctga gtttcgaggg gatgcagacg
     1021 ttcctcgatc agaagaacat ctccaagtac tactggcccg aaaggctcga ggtgatcgac
     1081 gagatccccc gtaacgtggt cggtaagatc cagaagttca agctgcgcga gcgggccgcg
     1141 gggctcacct cgcagcgcac caagtgaccg ccaaacaacc gagcagcagg cacggccgaa
     1201 gaggagaacg ggtggcaacc acgaccaaca ccgaatacga ggcgctcgtc gcggacgtca
     1261 cagcctgggt cgagggtccc ggcgaagagt gggcgaggcg agtcgaatcc accggcacgg
     1321 ttcccgacgc cctcttctcc gaactgcggg aacgcggcta cctgagcatg gcggcgccgg
     1381 tcgaactcgg cggccggggg ctcagcttca gccagtggat ggggctgatg gaggtcttct
     1441 cccgctccca cgcctccatc cgcatgctcg tgcacgtcgt caacggcacg tggcgcgcga
     1501 tggtgcccta cgcgaacgcc cagcaggtcg agaagtacat caagccgtcg atcttgggcg
     1561 agaaactcat cgccttcacc ctgaccgagc cgggcaacgg caccggcgcc gacatcagct
     1621 gcagcgtgcg gcgcgaaggc gacacctact acctctcggg ccagaaacac ctcatcacgt
     1681 tcggcttgaa atgcgactgg tggctgctct tcgcccggct cgaaggcacc accgggggcg
     1741 accagctcgt cggccttctc gtcgatcgcg gcgcggccgg tgtgaccgcc accgacatgg
     1801 gcgagacgat gggggtgcac ggcacagacc acgcgctcct cacgttcgag aacacgcctg
     1861 ttcccgtcgc acaacgcctc ggcgaggaag gcgacgggct cgagatggcg ctgggcgggt
     1921 ttttgacccc cagccgcgtc tccgtcgcga tgagctgcgt cgggctcgcc gaacgggcgc
     1981 aacagctcgc cgtcgactat gcggcaacga gggtcacctt cggcaagccg atcgcgaagc
     2041 gccaggcgat cgcgttcatg atcgccgaga accaggccga catcgaggcc gccaagcagc
     2101 tcgtgctgca cgccgctcgc cgatgggacg cgggcgaccc ggaggcctcc gccctctcct
     2161 ccatggcgaa gatcgtcgcc gtcgacatgc tcggccgcgt caccgacaag gcgttgcagg
     2221 tgcacggcgg catcggctat tggaagagtt ccgccatcga gcgggtctac cgggatgcgc
     2281 gtgcgcagcg cttcgaagag ggcaccaacg agatccagaa gaccgtcgtc gcccgcgaca
     2341 tcttccgggc gcgcgaggcg gcccggtcgg ggggcgcacg atgagcgacg ccgccgggcc
     2401 ccggcaaggg gaggggcgct acgcgggccg catcgccctc atcaccggtt cgtcgcgcgg
     2461 catcgggcgc accctcgcgc tggggctcgc cgcgcaaggc gcgacggtcg tcgtcaacta
     2521 ccgcaagaat gcggatctcg ccgccgaagt cgtggccgag gcgaagcgcc tgggcggcga
     2581 cgggatcgcg gtcggcgccg atgtcgagca gcccgacgag atcacccgca tgttcgacac
     2641 ggtcgccgac acgtacggcc gactcgactt cttcgtcaac aatgccgcgg ccgcggcctt
     2701 caagcgcatc ctcgacctga agccgcacca cctcgaccgc tcctatgcca ccaacgtgcg
     2761 acccttcgta ctcggtgccc agcaagccgt cacgctgatg gatcggggcg ggcgcatcgt
     2821 cgccgtctcc agctacggca gcatccgggc gttcccgacg tatgcggcgc tcggctccta
     2881 taaggcggcc atcgaatcgt tcatccgata catggccgtc gagttcgcgc cgtacggcat
     2941 caacgtgaac ggggtgaacg gcgggcttat ccaatcggac tcgctcgact tcttctattc
     3001 cgttcccggc atgtccccga tgcagaaggt tctcgacacg gtgccgctcg gccggcccgg
     3061 cacggtgcaa gacatggccg acgcgatcga attcttgctc tccccgcact ccgcctacat
     3121 cacgggtcag acgctcgtcg tcgacggcgg catgacggtc gccgccgcgc cgttctggcg
     3181 cgacacgacg agtccgctgg gcattcccga gcggccgacc aggggctgag caccgtgccc
     3241 gagacgacct tcccccggct tcgcgagccg gtgacgatcg gctccctgcg gctgccgcac
     3301 cgcgtcatca tgggctcgat gcacctcaac cgagagcgcg ggccggccga cgccctcgcc
     3361 gcgttctacg tcgaacgggt acgaggcggc gccgccctca tcgtgaccgg cggtgccgcc
     3421 gtcagccggg tgggtgccgg cagcagcgcc tattcgctca tcaacgaggc gccggatcaa
     3481 gagacgtggc aggtcgtggt cgacgccgtg caccaggcgg gcggcacgat cgcgctgcag
     3541 ctcttccatg ccggccgata cgccggcaca gaggcgttcg gcctcacccc cgtcgccccc
     3601 tccgccgtct acagtcggtt ctcgggctgc aagcccgaag cgctcaccga agagggggtg
     3661 cggcgcaccc tcgatgactt cgccgcgggg gcgaaggttg cggccgggct gggcttcgac
     3721 gcgatcgagg tgatgg
//
LOCUS       DASTNL010000155         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_662468, whole genome shotgun sequence.
ACCESSION   DASTNL010000155 DASTNL010000000
VERSION     DASTNL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662468"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VFQ96_04820"
     CDS             complement(<1..1120)
                     /locus_tag="VFQ96_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257145.1"
                     /translation="MNPRPRTLESLSHRELTAWRNALFVAFGLSGVALSTWVARIPSV
                     RDDLSLSTFAVGLIMLASSGGSILGLIAAPPLLTRLGPRRAIGAMLVADAAAMVAMGF
                     GTSTLGLVSVVLAGLVVFGFGAGAADVTMNVDAASAEKALGKTILPLMHGCWSGGTVV
                     GAGVGAAAAAWHLPVALHMTGVAVLIAAGILVAIRFIPNLLPADAELTVTGSIITPVV
                     PPAGWREKLRIRVAVWQDTRLLLIGLVMLGMAFAEGAANDWIALATVDGHHQSNAAGA
                     IFLGIFVTAMTVGRVAGGPFVDRFGRVAAVRGTAVLGVVGLLLFILSDLPWLAAVGTV
                     LWGLGASLGFPLGMSAAADDQRNAAARVSAVAMIGYCAL"
     gene            1258..>3193
                     /locus_tag="VFQ96_04825"
     CDS             1258..>3193
                     /locus_tag="VFQ96_04825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEU0257146.1"
                     /translation="MPDSAPHTATRSGIALDELDPDIRPQDDLFRHVNGRWIARTQIP
                     ADRARYGSFLILTEEAQKAIRAIIEEAQGAEPGSEARKVGDLYASFMDTAAIEARGAE
                     PLAAGLAAARAVDSIPGLLRTIGRLEADGIGGFLQLFVDNDPGAPERYVVFAEQDGIG
                     LPDESYYREESFQKVREAYRGHIERMFTLAGLGDAAARAARVFELEKAIAHAHWDNVR
                     SRDSEATYNLRSWAQVRALAGGAEDSGQVDGVGPAESALVEASPDLDQWRDALGAPPA
                     SLDEIVVRQPSFLHELAGLFTADRLDAWRDWLAWQVIHAHAPYLSQAFVDENFDFYGR
                     TLSGTPQLRERWKRGVSLVEGALGEAAGRVYVERHFPPAAKASMDSLVANLIEAYRQS
                     IGGLAWMGAKTRARALEKLDKFTPKIGYPTKWRDYSSLEIDAGDLIANVRATSRFEFR
                     RELGKIGRPIDRDEWFMTPQTVNAYYNPGFNEIVFPAAILQYPFFDPDRDDAANYGAI
                     GAVIGHEIGHGFDDQGAKYDGDGRLVNWWTPDDQAAFEARTASLVAQYNELSPRQVPD
                     HHVNGALTIGENIGDLGGLAIAWRAYLLSLDGAEPEVIDGMTGAERFFLSWAQSWQQK
                     GRDEEVIRLLAIDPHSPDEFR"
BASE COUNT          538 a         1071 c         1126 g          458 t
ORIGIN      
        1 gcagcgcgca atagccgatc atggcgacgg cgctcacgcg ggccgcggcg ttgcgctggt
       61 cgtcggcggc cgcggacatg ccgagcggga agccgagtga ggcgccgagc ccccacagca
      121 cggtgccgac ggcggcgagc cagggcaggt ccgagaggat gaagagcagg agccccacga
      181 cgcccagcac ggccgtgccg cgcaccgcgg cgacccgacc gaagcggtcg acgaacgggc
      241 cgccggcgac ccggccgacc gtcatcgcgg tgacgaagat gccgaggaag atcgcgcccg
      301 cagcgttcga ctgatggtgc ccgtccaccg tggcgagggc gatccagtcg ttcgcggcgc
      361 cttcggcgaa cgccattccg agcatcacga gcccgatcag aagaagccgg gtgtcttgcc
      421 acacggcgac ccggatgcgc agcttctccc gccagccggc cggcggcacg accggcgtga
      481 tgatgctgcc cgtcacggtg agctccgcgt cggccggcag gaggttcggg atgaaccgga
      541 tcgcgaccag gatgcccgcg gcgatcagca ccgcgacccc cgtcatgtgg agggcgacgg
      601 gcaggtgcca cgcggccgca gccgccccga cgcccgcgcc gacgacggtg ccgccgctcc
      661 agcaaccgtg catgagcggc aggatcgtct tgccgagcgc cttctcggcg ctcgccgcgt
      721 ccacgttcat cgtcacgtcg gcggcgccgg ctccgaagcc gaagacgacg agcccggcga
      781 ggacgacgct gacgagaccg agggtcgagg tgccgaaccc catcgcgacc attgcggcgg
      841 cgtccgcgac gagcatggcg ccgatcgccc gccgggggcc taggcgggta aggagcggcg
      901 gggcggcgat aaggccgagg atcgacccgc cggaggaggc cagcatgatg agcccgaccg
      961 cgaaagtcga gagcgacagg tcgtcgcgca ccgaggggat cctggcgacc caggtcgata
     1021 acgcgacgcc gctcaggccg aacgccacga aaagcgcgtt gcgccacgcc gtcaattccc
     1081 gatggctcag cgactcgagg gtgcgaggcc tgggattcat cacggcgggt tcctgtgtct
     1141 tctctatttt tccgggatct tgggccgaat atgcccgcgg ttaacgctag cggctcgccc
     1201 ggcgggcagg gcgccgttct cgcttcgcgc aaagcgcgac tcccataggc tgtacccatg
     1261 cctgactccg caccgcatac ggccacgcgt tccggaatcg cactcgacga actcgacccc
     1321 gacatccgcc cgcaagacga cctcttccgg cacgtcaacg gcaggtggat cgcacgcacg
     1381 cagataccgg ccgaccgggc ccgctacggc tcgttcctta ttctcaccga agaggcacag
     1441 aaggcgatcc gcgccatcat cgaagaggcg cagggggcag aaccgggcag cgaggcccgc
     1501 aaggtcggcg acctgtacgc cagcttcatg gacacggcgg cgatcgaggc gcgcggggcc
     1561 gagccgttgg ctgcgggcct tgccgcggcg cgtgccgtcg actcgattcc cgggctgttg
     1621 cgcacgatag gccggctcga agccgacggc atcggcgggt ttctgcagct cttcgtcgac
     1681 aacgaccccg gagcgccgga acggtacgtc gtcttcgccg agcaagacgg catcggcctg
     1741 cccgacgaga gctactaccg cgaggagtcg ttccagaagg tccgcgaggc gtaccgcggc
     1801 catatcgagc ggatgttcac ccttgccggc ctcggcgatg cggcggcgcg tgccgcgcgg
     1861 gtgttcgagc tggagaaggc gatcgcgcac gcgcactggg acaacgtgcg ttcccgcgac
     1921 agcgaggcga cctataactt gcgcagctgg gcgcaggtgc gcgccttggc cggcggggcg
     1981 gaggattcgg ggcaggtcga cggtgtcggg cccgcggagt ccgcgttggt cgaggcctcg
     2041 ccagatctcg accagtggcg agacgcgttg ggcgcgccgc cggcatccct cgacgagatc
     2101 gtcgtgcgac agccgagctt cctccacgag ctggcgggcc tcttcaccgc cgaccggctc
     2161 gacgcgtggc gagactggct ggcctggcag gtcatccacg cgcacgcacc gtacctttcg
     2221 caggctttcg tcgacgagaa cttcgacttc tacggccgca cgctctcggg caccccgcag
     2281 ctgcgggaac gttggaagcg tggcgtctcc ctcgtcgagg gggccttggg tgaggcggcc
     2341 gggcgggtgt acgtggagcg gcactttccg cccgccgcga aggcaagcat ggattcgctc
     2401 gtcgccaatc tgatcgaggc ctaccggcag agcatcgggg gcctcgcctg gatgggcgct
     2461 aagacccggg cgcgggcgtt ggagaaactc gacaagttca cgccgaagat cggttatccg
     2521 acgaagtggc gcgactattc gagtcttgag atcgatgcgg gcgacctcat tgccaacgtg
     2581 cgggcgacga gccggttcga gtttcggcga gagttgggca agatcgggcg gccgatcgac
     2641 cgcgacgagt ggttcatgac gccgcagacc gtcaatgcct actacaaccc cgggttcaac
     2701 gagatcgtct tcccagccgc gatattgcaa tatccgttct tcgaccccga tcgcgacgac
     2761 gcggcgaact acggcgcgat cggcgccgtg atcgggcatg agatcgggca cgggttcgac
     2821 gatcagggcg ccaagtacga cggcgacggg cggctcgtca actggtggac ccctgacgat
     2881 caggcggcgt tcgaggcccg cacggcctcg ctcgtcgcgc agtacaacga gctgtcgccc
     2941 cggcaggtgc ccgatcatca cgtgaacggg gcgttgacga tcggcgagaa tatcggcgac
     3001 ctcggcggtc tggcgatagc gtggcgcgcc tacctcttgt ctctcgacgg tgccgagcca
     3061 gaggtcatcg acggcatgac cggcgcggaa cggttcttct tgtcgtgggc gcagtcgtgg
     3121 cagcagaaag gccgcgacga agaggtcatc cgcctcttgg ccatcgaccc gcattcaccg
     3181 gatgaattcc gct
//
LOCUS       DASTNL010000156        10815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_291834, whole genome shotgun sequence.
ACCESSION   DASTNL010000156 DASTNL010000000
VERSION     DASTNL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291834"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..765
                     /locus_tag="VFQ96_04830"
     CDS             4..765
                     /locus_tag="VFQ96_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257147.1"
                     /translation="MSVVRDGGAILSMVDWSVNPEDRWVVLGPNGAGKTTLLSVAATS
                     LHPTSGVVEVLGERVGSTDLSGLRPHIGYVANAVARRVPRRESVLDVVLTAAYSVFGR
                     WHEEYEEIDVRRARRVLAEWRLERFERREFGTLSDGEQKRVLIARAVMTDPEVLLLDE
                     PAASLDLGSREELLGLLGGFASDPRSPAIVMVTHHVEEIPPAFTHVLLLAQGGVVAAG
                     RIPATLTAANLEEAFGLPVSLVANSGRYSATARAR"
     gene            890..1141
                     /locus_tag="VFQ96_04835"
     CDS             890..1141
                     /locus_tag="VFQ96_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU0257148.1"
                     /translation="MKSGIHPDYAPVVFHDLASGESFLTQSTVKSDKTVEWEDGNTYP
                     VVDVEISSASHPFYTGKQRILDSAGRVEKFNQRFKAFGK"
     gene            complement(1166..1849)
                     /locus_tag="VFQ96_04840"
     CDS             complement(1166..1849)
                     /locus_tag="VFQ96_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEU0257149.1"
                     /translation="MQSWADRLGVFDVETTGVNVDAVRIVSAHVGVLDADGRLAVRHD
                     WLVDPGVEIPPQAQAVHGISTERAKREGMPAASAVGQIVETLARLFEDGVAVTAYNAP
                     YDFTVLDREAARYGIAGLDGPRPVIDPLVIDRQVDKYRRGKRTLQVTCEHYGVALDNA
                     HDASSDAAAAGRLAQELARRFPQLVVPATQLHDDQVAWSRAQAEDFEAYMRRVKDPSF
                     RAAVGWPVR"
     gene            1923..2858
                     /locus_tag="VFQ96_04845"
     CDS             1923..2858
                     /locus_tag="VFQ96_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0257150.1"
                     /translation="MDPTPDLADPRAEEGNHHETRIGAGVTHWWQYGPADAATTAIFV
                     HGFRGDHHGLGRVAGQLQRTFPEPVRLVIPDLPGFGRSTAHGGALDLPAYARWLRGFT
                     AAVAGETRVLLIGHSFGSVVAAAAVACDPEGRAPWRLALINPIGAPALAGPRRVGTAL
                     AVAYYQLAAHLPERAGFALLQNPAVVRVMSIAMAKTRDPALRRWIHDQHRRYFSLFES
                     RHSLLQAFRASVENDVSMFAGRIAVPTLLIAADRDDITPLAAQRRLRTLFADARLAVI
                     HGAGHLVHYEAPALAAWEIARFASSDGCPAAGSRP"
     gene            2872..4005
                     /locus_tag="VFQ96_04850"
     CDS             2872..4005
                     /locus_tag="VFQ96_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0257151.1"
                     /translation="MTRLVVDCRYVRVGRHDGISRYTAGVVTALLRRHPVTMLICDKR
                     QLTMLPKAPWAMARRPTSVWEPLVALRVNRLRPDVVFSPMQTMGSIGRRYRLILTVHD
                     LIYYRHRTPPRDLPAFVRLLWRFYHLSWWPQRLLLNGADAVVTVSETTKSLIAEHRLT
                     RRPVEVVPNAADELPGSPADAAPHDGPQVPGPGRDLVYMGSFMPYKDVDTLVRAAALL
                     PGYRLHLMSRAGGRERDRLTSMAKGAVLVFHDGASDAEYLATLRQAAALVTASRDEGF
                     GIPLLEAMRAGTPVVVSDIPIFREIGGDAALYAEPGDAAAFAARIRLLRDPAVRAGLI
                     EAGRKRAADFSWDRSAATLLGLARRLVAGPREDPRACQEEHEG"
     gene            complement(4024..4620)
                     /locus_tag="VFQ96_04855"
     CDS             complement(4024..4620)
                     /locus_tag="VFQ96_04855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0257152.1"
                     /translation="MTRLALVRHGQTDWNLERRIQGSSDIPLNAVGRAQARRTSDFLA
                     GRHWDAVFSSPLSRAWETAEIIATGLGLASPTAVQGIQERSYGGAEGLTSAEITAKYP
                     GDAAIPGRESRKDVVARALPALLRLAEEHPRCALVVVSHGAVIGSLVRHLTDHALPGP
                     GNLIANGSVHEFLYRRGALVLDKFNQTPDDDVVAAAAP"
     gene            complement(4535..5491)
                     /locus_tag="VFQ96_04860"
     CDS             complement(4535..5491)
                     /locus_tag="VFQ96_04860"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU0257153.1"
                     /translation="MAAPNPDPKVLGRAVALLARRRIAVLTGAGVSTDSGLPDYRGAG
                     SPPRHPMTIAEFRASAAARRRYWAGSQRGWRLFSGARPNTGHRALALLGRAGAVTGVA
                     TQNVDGLHLAAGSRKVVPLHGTMHRVVCLTCGQVFARDAVAERIRRDNPWFDDDVAQL
                     APDGDAEVGDVERFVVPSCTVCGGILKPEVVFFGETIPRDVFRLASSLVAASDALLVV
                     GSSLVVNSGIRVLEQARRRRLPVVIVNRGATRGDGRAVVKLDAGASQTLAVLCERLVP
                     GGNGIRTQRKEGGPRDKARSRAPRADRLEPRAAHPRLERHPA"
     gene            5616..6434
                     /locus_tag="VFQ96_04865"
     CDS             5616..6434
                     /locus_tag="VFQ96_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0257154.1"
                     /translation="MRVQRVGGVDDAALADYTGLTDVALRRTLEPAGGLYIAESTKVL
                     ARALEAGHRPRSVLVQEKWLPDVTDLLAGFPRTANVPVYVGGDELLSEVTGFHVHRGA
                     LASMHRPPLPEARSLLRAARLVVIVEDVVDHTNVGAIFRSAAGLGADAVLVTPRCADP
                     LYRRSVRVSMGTVFQVPWTRLPDWPEGADLVHEAGFHIAALALRDDAVGLDEFCRHAP
                     KKVAVLVGTEGDGLSRQAVHDADSVVRIPMLHGVDSLNVAASSAVALWALRRRR"
     gene            6431..7663
                     /locus_tag="VFQ96_04870"
     CDS             6431..7663
                     /locus_tag="VFQ96_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0257155.1"
                     /translation="MRRRHRRPGRHIRTSILSGLAIFLAAILAYGTYAVVAPVPPVEA
                     QESTSSIQGRPATVTWPSRGVAAVGVVGADGLIAQHGSNSSIPTASMAKTVTALVLLA
                     KKPLSGPTDEGPKITFTAKDLAIEKQVIKNDGSWAPVKPGVAMTERQALEAMLLPSAN
                     NYAISLADWAFGSVSAFTRSANAWLGRRGITHTHMADASGLSANTRSTPADLVAIGKL
                     VRANAVLSSIVATKKATVPGAGTLHNTNGVLGVDGIDGVKTGSTKAAGYCLMFSTTVT
                     KDSHTVTIVGVVAGEPSFSRLFSDVTKLVASLRSGFREVDLAGPGITVGEYQTPWGQA
                     IPLVVATPTTALVWSNTHVTVRIVTRTVTTAPAGTTVGAVTFRVGRQVLASRTVQLGS
                     ALGPPPLTWLLAHPPERR"
     gene            7692..9479
                     /locus_tag="VFQ96_04875"
     CDS             7692..9479
                     /locus_tag="VFQ96_04875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0257156.1"
                     /translation="MTAISEGERRGSEGFAAGRLAPAFPARAPWGTAERLRAWQEEAL
                     ERYFAARPRDFLAAATPGAGKTTFALRLAAELLARRVVERITVVAPTEHLKRQWADAA
                     ARAGIRLNPAFHNADHRYARHYRGIAVTYAQVAANPAVHRDLTAEYRTLVILDEVHHA
                     GDALSWGDAIREAFGRARRRVSLTGTPFRSDTAPIPFVTYLPDDDGIRTSQTDYSYGY
                     GRALGDGVVRPVLFLVYAGQMRWRTRAGDEMEARLGEDNTKDVTAQAWRTALDPGGEW
                     IPAVLDAADRRLTHVRQQIPDAGGLVIATDHDHARAYAALLRQITGERPTVVVSDEKE
                     SSDRIARFAADGSRWMVAVRMVSEGVDVPRLCVGVYATSAATPLFFAQAVGRFVRARR
                     RGETASIFLPNVPLLTALASSLEKERDHALDRVSSGETPEEDLVAEANRAESASDGLL
                     SDEFSFQPISSEANLDSVLYDGDEFGGYAGPRTEEELDFLGLPGILEPEQVRDLLKSR
                     QARQARRAAARKPEEQQAPAPLYRTLREQRRLLNSLVGLYAKNTGAPHGLVHADLRRS
                     CGGPAVGQASVSQLQARIDLIRARLGHAR"
     gene            complement(9490..10401)
                     /locus_tag="VFQ96_04880"
     CDS             complement(9490..10401)
                     /locus_tag="VFQ96_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HEU0257157.1"
                     /translation="MDDDRYGADVLAGDWRQAGRRQAARVVAAAGLVVEDSLSGYCGA
                     VIRVEGGAVELEDRHGRRRVFPLGGGYLVDGEPVVLTVPGPTLPRRTPATGRAAAGTA
                     MGPAPNAGAGTPRPRTASGSFALAPRPARVARASRIFVEGRHDAELVEKVWGDDLRVE
                     GVVVEYLEGVDNLAEMLRRFRPTPVRRAGVLVDHLVAGSKETRIVADARAQASAGSLL
                     VVGHPFIDVWQAVKPARLGLAAWPHIPHSVEWKHGICQALGWPHAGQADIAAAWRRIL
                     ATVRDYADLEPAMLGRVEELIDFVTAG"
BASE COUNT         1584 a         3899 c         3769 g         1563 t
ORIGIN      
        1 ggcgtgagtg tggtgcgcga cggcggtgcg atcttgagca tggtggactg gtcggtgaac
       61 cccgaggacc gttgggtcgt gctgggcccg aacggggcag gcaagacgac gctcttgagt
      121 gtcgcggcga cgagcctgca cccgacctcc ggcgtggtcg aggttctggg ggagcgggtc
      181 ggctccacag acctctccgg gctgcgcccg cacatcggct acgtcgcgaa cgccgtggcc
      241 cgtcgggtgc cgcgccgaga aagcgtgctc gacgtggtgt tgacggccgc gtattcggtc
      301 ttcggccgct ggcacgagga atatgaggag atcgacgtgc gccgggcgcg ccgggtgctc
      361 gcggaatggc gcctcgagcg gttcgaacgg cgcgagttcg gcacgctgag cgacggtgag
      421 cagaagcggg tgctcatcgc acgggccgtg atgaccgacc ccgaggtgct gctcctcgac
      481 gagccggcgg cgagtctcga cctcggctcg cgggaagagt tgctgggtct cttgggtggt
      541 ttcgcgagcg acccgcgctc gccggcgatc gtcatggtga cccaccacgt cgaggagatc
      601 ccgccggcgt tcacccacgt gctcctgctc gcgcagggtg gcgtcgtcgc cgccgggcgg
      661 ataccggcga cgctcaccgc ggccaacctg gaggaggcct tcggcttgcc cgtgtctctg
      721 gtcgcgaact ccggccggta cagcgccacg gcgcgagcca ggtgagcccg gcacgctgct
      781 aagataggtg gtcggctcgt tccggcgttc ctctcgtcgg cggccgcggt cgtcgcccgc
      841 cgcagttcgc tctgccggca gttcgttatc gatgcagcaa ggaagtctga tgaaatctgg
      901 cattcacccc gactacgccc ccgtcgtctt ccacgacctc gcctccggcg agtccttctt
      961 gacacagtcc accgtgaaga gcgacaagac ggtcgagtgg gaagacggca acacgtaccc
     1021 cgtcgtcgat gtcgagatct cgtcggcctc gcaccccttc tacacgggca agcagcgcat
     1081 tctcgactcg gccggccggg tggagaagtt caaccagcgg ttcaaggcgt tcggcaagta
     1141 agtagccgcc gggccgggcc ggcgcttagc gcaccggcca gcccacggcg gcccggaacg
     1201 acgggtcttt gacccggcgc atgtaggcct cgaaatcttc ggcctgcgca cgagaccacg
     1261 cgacctggtc gtcgtgcagc tgggtggccg gcaccacgag ctgtgggaaa cgccgcgcca
     1321 gttcctgggc gagccgcccc gcggcggccg cgtcggacga ggcgtcgtgc gcgttgtcga
     1381 gcgccacgcc gtaatgctcg cacgtgacct gcagggttcg tttgcctcgc cggtacttgt
     1441 cgacctgccg gtcgatgacg agcgggtcga tgacgggccg cggcccgtcg aggccggcga
     1501 tgccgtaccg tgcggcctcc cgatcgagca ccgtgaagtc gtagggcgcg ttgtaggcgg
     1561 tgactgccac gccgtcctcg aagaggcggg ccaaggtctc gacgatctgg ccgaccgccg
     1621 aagcggccgg catgccctcg cgctttgccc gctccgtgct gatgccatgc accgcctgag
     1681 cctgcggcgg gatctcgacg ccgggatcga ccagccagtc gtgacgcacc gccagtctgc
     1741 cgtcggcgtc gagcacgccg acgtgcgccg aaacgatacg caccgcgtcg acgttcaccc
     1801 cggtcgtctc cacgtcgaac acacccagac gatcagccca cgattgcata ccggaagcct
     1861 agggcctgcc ggcgacgggc cgggcacgca cgggcgccca cgccgatgcc tagactggcg
     1921 aggtggatcc gacccccgac cttgccgatc cgcgggccga agagggaaac caccacgaga
     1981 cacggatcgg ggccggcgtc acgcactggt ggcagtacgg gccggctgac gcggccacga
     2041 cggcgatctt cgtgcacggc tttcgcggcg accaccacgg cctcgggcgc gtcgccgggc
     2101 agctgcaacg cacgttcccc gagccggtgc ggctcgtcat ccccgacctg ccgggcttcg
     2161 gccgatccac cgcgcacggc ggcgccctcg acctgccggc ctatgcgagg tggctgcgcg
     2221 ggttcacggc cgccgtggcg ggcgagaccc gtgtcctcct catcgggcat tcgttcggct
     2281 ccgtggtcgc ggcggcggcc gtcgcctgtg accccgaggg gcgggcgccc tggcgtctgg
     2341 ccctcatcaa cccgatcggt gccccggctc tggccggccc gcggcgcgtc ggcacggcgc
     2401 tggcggtcgc ctattaccag ctggccgccc acctgcccga acgggccggg ttcgccctgc
     2461 tgcagaaccc cgccgtcgtg cgcgtcatga gcatcgcgat ggcgaagacc cgcgaccctg
     2521 cgctgcgtcg atggatccat gaccagcacc ggcggtattt cagcctcttc gagagccggc
     2581 acagcctcct tcaggcgttc cgcgcctcgg tcgagaacga cgtcagcatg ttcgccggcc
     2641 gcatcgccgt accgacgctc ctcatcgccg ccgaccgcga cgacatcacc cccctcgccg
     2701 cgcaacgtcg cctgcgaacg cttttcgccg acgcccgcct cgccgtgatc cacggcgccg
     2761 gacaccttgt gcactacgag gcacccgcgc tggcggcgtg ggagatcgcc cggttcgcat
     2821 cctccgacgg atgcccggca gccgggtcgc gcccgtgaag aaggtcaggc ggtgacaagg
     2881 ctcgtcgtcg actgtcgtta cgtgcgcgtc ggccggcacg acggtatcag ccgatacacg
     2941 gcgggcgtcg tgacggcgct tcttcgccgt cacccggtca cgatgctgat ctgcgacaaa
     3001 cgccagctga cgatgttgcc aaaggcgccc tgggctatgg cccgacggcc gacgagcgtg
     3061 tgggagcccc tggtggcgtt acgcgtcaac cggctaaggc ccgacgtcgt cttcagcccg
     3121 atgcagacca tgggctcgat cgggcgtcgc taccggctca tcttgaccgt gcacgacctg
     3181 atctactatc gccaccgcac cccgccccgt gacctgcctg cgttcgtgcg cctcctgtgg
     3241 cgcttctacc acctgtcgtg gtggccgcag cggctcctct tgaacggggc cgacgccgtc
     3301 gtgacggtct ccgagacgac aaagtcgctc atcgcggaac atcggttgac gcgccgcccg
     3361 gtcgaggtcg ttccgaacgc cgccgacgag ctgcccggca gccccgcaga cgctgccccg
     3421 cacgacgggc cgcaggtgcc cggcccgggc cgtgacctcg tctacatggg ctccttcatg
     3481 ccgtacaaag acgtcgacac gctcgtgcgc gcggcggcgc ttctgcccgg ctaccgcttg
     3541 cacctcatga gccgcgccgg cgggcgtgag cgcgaccgcc tgacctcgat ggcgaagggc
     3601 gccgtgctgg tcttccacga cggcgccagc gacgccgagt acttggcgac cctccggcag
     3661 gcggctgcgc tcgtcaccgc ctcacgggac gaaggcttcg gcatccccct cctcgaggcg
     3721 atgagggcgg ggacaccggt cgtcgtgagc gacatcccga tcttccgcga gatcggcggc
     3781 gacgccgccc tctacgccga gccgggtgac gccgcagcgt tcgcggcgcg gatccggctg
     3841 ctgcgggacc ccgccgtgcg cgccggcctg atcgaggccg ggcgaaaacg ggccgccgac
     3901 ttcagctggg atcgctcggc cgcgacgctg ctgggtctcg cgcgccgtct cgtcgccggc
     3961 ccgagagaag acccgcgcgc ctgccaggag gagcacgagg ggtgagcgcc ctgcgagagc
     4021 acgttacggc gcggcagccg cgacgacgtc gtcgtcgggc gtctgattga acttgtcgag
     4081 caccagggca cctcgacggt agagaaactc gtgcacggag ccgttggcga tgaggttgcc
     4141 gggcccgggc agggcgtggt ccgtcaggtg ccgcaccaac gagccgatga ccgcgccgtg
     4201 gctgacgacg acgagcgcgc accgcgggtg ctcctcggcg aggcgcagca acgcgggcaa
     4261 ggctcgggcg accacgtctt tccggctctc ccggccgggg atcgccgcat cgcccggata
     4321 cttcgccgtg atctcggctg aggtgagccc ctccgccccg ccgtacgagc gctcctggat
     4381 gccttgcacg gccgtgggcg aggcgaggcc gagccccgtc gcgatgatct cggccgtctc
     4441 ccaggcgcgc gagagagggc tcgaaaacac ggcgtcccag tgccggcccg caaggaaatc
     4501 gctcgtccgt ctggcctgcg cacggcccac ggcgttaagc gggatgtcgc tcgagccttg
     4561 gatgcgccgc tcgaggttcc agtcggtctg cccgtggcgc acgagagcga gccttgtcac
     4621 gtggccctcc ctccttgcgt tgggtacgaa taccgttccc cccgggaacg aggcgttcgc
     4681 acagcaccgc cagcgtctgc gacgccccgg cgtccagctt aacgaccgcc cggccgtcgc
     4741 cgcgcgtggc gccgcgattc acgatgacga cgggcagccg ccgccgcctc gcctgctcga
     4801 gaacgcggat gccggaatta accacaaggg acgagccgac gacaagcaac gcatccgacg
     4861 ccgccaccag cgaggacgcg agccggaaca catcgcgggg aatcgtctcg ccgaagaaga
     4921 ccacctcggg cttcaggatg ccgccgcaca cggtgcagga cggcacgacg aaacgctcga
     4981 cgtcgccgac ctccgcgtcg ccgtcgggtg ccagctgcgc gacgtcgtcg tcaaaccacg
     5041 ggttgtcacg gcggatccgc tccgccaccg cgtcgcgcgc gaacacttga ccgcacgtca
     5101 gacacacgac ccggtgcatc gtgccgtgca aaggcacgac cttgcgactt cccgcggcca
     5161 ggtgcaggcc gtcgacgttc tgagtggcga cccccgtgac ggcgccggca cgccccaaga
     5221 gcgccagcgc ccggtggccg gtgttcggcc gggccccgct gaagaggcgc cagccacgtt
     5281 ggctgcccgc ccaataccgt cggcgggcgg cggcagaggc ccggaactcg gcgatcgtca
     5341 tgggatgcct aggcggcgat ccggcgccac gatagtccgg caggcccgaa tcggtgctga
     5401 cccccgcacc ggtcaagacg gcgatgcgac ggcgcgccaa aagcgcgacg gcgcgcccca
     5461 gcaccttggg atccggattc ggcgccgcca cgtcattagt ctaggaacac cacgttccgc
     5521 catgcgcgct gcgcggttgg gatgacgcgc cccacctgcc cgtcgcatcg cccgcgtgtc
     5581 ggcgggttcc gggcgacggc cggcgagggg gtggcatgcg ggtccagagg gtcggcgggg
     5641 tcgacgatgc ggcactggcc gactacacgg gcctcaccga cgtcgcattg cgtcgcacgc
     5701 tcgagccggc gggcgggttg tacatcgccg agtcgacgaa ggtcttggcc cgggcgctcg
     5761 aagccgggca ccggccgcgt tccgtcctcg tgcaagagaa gtggctgccc gacgtgacgg
     5821 atcttctcgc aggcttcccc cgcacggcga acgtgcccgt gtacgtcggc ggcgacgagc
     5881 tcttaagcga ggtgaccggg ttccacgtgc accgcggggc cctcgcctcg atgcaccggc
     5941 cgccgcttcc cgaggcccga agccttctcc gggcggcccg cctcgtcgtc atcgtcgaag
     6001 acgtcgtcga ccacacgaac gtcggggcga tctttcgctc cgccgcgggc ctgggggccg
     6061 atgccgtgct cgtcacaccc cgctgtgccg acccgctcta ccgccgcagc gtgcgggtca
     6121 gcatgggcac cgtcttccag gtgccctgga cccggttgcc cgactggccg gagggcgccg
     6181 atctcgtgca cgaggcgggc ttccacatcg cggcgctcgc cttgcgcgac gatgcggtcg
     6241 gcctcgacga gttctgtcgg cacgcgccga agaaggtggc ggtgctggtc ggcaccgagg
     6301 gcgacgggct cagccgccag gctgtgcacg acgctgattc cgtggtacgg atccccatgc
     6361 tgcacggcgt ggattcattg aacgtcgccg cgagttccgc ggtcgcactc tgggccctgc
     6421 gaaggagacg atgagacgca gacaccgccg gccaggccgg cacatccgca cgagtatcct
     6481 gtcgggcctc gccatcttcc tcgcggcgat cctcgcctac ggcacctacg ccgtcgtcgc
     6541 gcccgtcccg ccggtcgagg cgcaggagtc gacgtcgagc attcaaggcc ggccggctac
     6601 ggtcacgtgg ccttcccgag gtgtggcggc agtcggcgtc gtcggcgctg acggcttgat
     6661 cgcgcaacac ggctccaact catccatccc gaccgcaagc atggccaaaa cggtgaccgc
     6721 gctggtattg ctcgcgaaga agccgctctc tgggcccacc gacgaggggc cgaagatcac
     6781 cttcacggcg aaagacctcg ccatcgagaa gcaggtcatc aagaacgacg ggtcgtgggc
     6841 gccggtgaag ccgggcgtcg ccatgacgga acgtcaggcg ttggaggcga tgctgctgcc
     6901 gtcggcgaac aactacgcga taagcctcgc cgactgggca ttcggctccg tgtcggcgtt
     6961 cacgaggtcg gcgaacgcgt ggctcggccg gcgcggcatc acacacaccc acatggccga
     7021 cgcgtcgggg ttaagcgcga atacgcgcag cacgcccgcc gacctcgtgg cgatcggaaa
     7081 gctcgttcgg gccaacgccg tgctctcgtc gatcgtcgcc acgaaaaagg cgacggttcc
     7141 cggcgcgggc acgctgcaca acacgaacgg cgtgctcggt gtcgacggga tcgacggggt
     7201 gaagaccggc agcaccaagg cggccggcta ctgcctgatg ttctccacca ccgtgacgaa
     7261 agacagccac accgtgacga tcgtcggagt cgtcgccggg gagccaagtt tcagccggct
     7321 gttctccgac gtcacgaagc tcgtggcaag cctgcgctcc ggcttccgcg aggtcgacct
     7381 ggcaggcccg ggcatcacgg tcggcgagta ccagacgccg tgggggcagg cgatcccgct
     7441 cgtcgtcgcg acgccgacga cggcgctcgt ctggtcgaac actcacgtca ccgtacgcat
     7501 cgtcacccgc accgtcacta ccgcgccggc cggcacgacg gtgggcgccg tcacgttccg
     7561 ggtcggcagg caggtgctcg cctcccgcac cgtgcagctg ggctcggccc tcggcccgcc
     7621 gcccctgacc tggcttctgg cgcacccgcc cgagcggcgg tgaccctgac gcccggcgtt
     7681 agagtcgttg ggtgacggcg atctcggagg gcgaacgacg cggctccgaa gggttcgccg
     7741 cggggcgcct cgccccagcg ttccccgcgc gcgcgccgtg gggcaccgcc gagcgcctgc
     7801 gagcgtggca agaggaggcc ttggaacggt atttcgccgc ccgcccgcgt gacttccttg
     7861 cggccgctac gcccggcgcg ggcaagacca cgttcgcgtt gcggctcgcc gccgaactgc
     7921 tcgcccggcg cgtcgtggaa cggatcacgg tcgtcgcgcc gaccgaacac ctgaagcggc
     7981 agtgggccga tgcggccgcg cgggccggta tccgcctgaa cccggcgttc cacaacgccg
     8041 accatcgcta cgcccgacac taccgaggca tcgcggtgac ctacgcacag gtcgcggcga
     8101 acccggccgt gcaccgcgac ctgactgcgg aataccgtac gctcgtcatt ctcgacgagg
     8161 tgcatcacgc gggcgacgcc ctcagctggg gcgacgcgat acgggaggcg ttcgggcgcg
     8221 cgaggcgtcg tgtctcgctg accggcacgc cgttccgctc cgacaccgcg cccataccgt
     8281 tcgtcaccta cctgcccgac gacgacggca tccgcacctc gcagacggac tacagctacg
     8341 gctacgggcg ggccttgggc gacggggtcg tgcgcccggt tctcttcctc gtctatgccg
     8401 gccagatgcg ctggcgcacc cgggcgggcg acgagatgga ggcccggctg ggggaggaca
     8461 acaccaagga cgtcaccgcc caggcatggc gaacggctct cgaccccggt ggcgagtgga
     8521 tccccgccgt gctcgacgcg gcggaccgcc gcctgaccca cgtgcgccag cagatccccg
     8581 acgccggtgg gctcgtcatc gcgaccgacc acgaccatgc gcgggcgtac gccgcactct
     8641 tgcgccagat cacgggggaa aggcccaccg tcgtcgtcag cgacgagaag gagtcgagcg
     8701 acaggatcgc ccggttcgcg gccgatggct ctcgctggat ggtcgcggtg cgcatggtct
     8761 ccgagggcgt cgacgtgccg cggctctgcg tgggcgtgta tgcgacgagc gccgcgaccc
     8821 cgctgttctt cgcgcaggcc gtcgggcggt tcgtgcgggc tcgcaggcgc ggcgagacgg
     8881 cgtcgatttt tctgccgaac gtgccgctcc tgaccgcgct ggcctcgagc ctcgagaagg
     8941 agcgggacca cgcgctcgac cgcgtctcct ccggcgagac gcccgaagaa gacctcgtgg
     9001 cggaggcgaa tcgggcggag agcgcctccg acggcctcct cagtgacgag ttctcctttc
     9061 agccgatcag ctccgaggcg aacctcgatt cggtgcttta cgacggcgac gagttcggcg
     9121 ggtatgcggg gccgcgcacc gaagaggaac tcgattttct cggcctgccg ggcatcttgg
     9181 agccggagca ggtgcgggat ctcctgaaga gtcggcaggc gaggcaggcc agacgcgccg
     9241 ccgcacgcaa acccgaggag caacaggcac cggcgcccct ctaccgcacg ctgcgggaac
     9301 aacgccggct cttgaacagt ttggtgggcc tgtacgcgaa gaacaccggc gcgccgcacg
     9361 gcctggtgca cgccgacctg cgccgctcct gcggcgggcc ggcggtcggc caggcgagcg
     9421 tcagccagct tcaggcccgg atcgacctga tccgtgcccg cctcggccac gcgcggtgac
     9481 cccgccgcct cagcctgccg tgacgaagtc gatgagttct tcgacgcggc cgagcatggc
     9541 cggttcgagg tctgcgtagt cgcgcacggt ggcaaggatg cgtcgccacg cggccgcgat
     9601 gtccgcctgg ccggcatgcg gccagccgag cgcctgacag atgccgtgct tccactcgac
     9661 ggagtgcggg atatgcggcc aggcggcaag ccccagccgg gccggcttca ccgcctgcca
     9721 cacgtcgatg aacggatgac cgacgacgag gagcgatccg gcgctggcct gcgctcgcgc
     9781 gtccgccacg atgcgggtct ctttcgaccc ggcgacgagg tgatcgacga gcacgccggc
     9841 ccgcctgacg ggcgtcggcc ggaaccgccg gagcatttcg gcaaggttgt cgaccccttc
     9901 caggtattcg acgacgaccc cctcgacacg caagtcgtcg ccccacacct tctccacgag
     9961 ttccgcgtcg tggcggcctt cgacgaagat ccgactggcg cgtgcgaccc gggcgggccg
    10021 cggcgcgagc gcgaacgacc cggaggcggt acgcggccgc ggcgtgcccg ccccggcgtt
    10081 aggcgcaggc cccatcgcgg ttccggcggc cgcccggccg gtcgcaggag tacggcgggg
    10141 gagggtcggg ccgggcaccg tcagcaccac cggctcgccg tcgacgaggt agccgccacc
    10201 gagcgggaac acccgtcgtc ggccgtgccg gtcttcgagt tccaccgcgc cgccctcgac
    10261 ccggatcacg gccccgcaat agccgctgag cgagtcttct acgacgaggc ccgcggcggc
    10321 gacgacgcgt gcggcctgcc gcctgcccgc ctggcgccag tcgccggcga gcacgtcggc
    10381 gccgtagcgg tcatcatcca ccgggccagc ctatccagcg catcggaggc tgtagggcgt
    10441 ttcgaggtcc cggcctccac gggcccggcg ccgagccgtc gcttcatcat cgcgcccgat
    10501 agcaacacgg ccgatcgaag gcgccgtgcc ggagccccgc gcgccgccgc gtcacgcatc
    10561 cggcagcgcc gatgcttcgg gagacggcct cgggccgact cggggatcca cggggccggt
    10621 gttgccggat gccctctcgc gggagcgtca gcgatcgagc aggcgatcga tggcgcgccg
    10681 gtcgcgtttc gtcggtcggc cggcgccgcg atctcggcgg ccgaagggca cggccgcgat
    10741 cgggtcgggt ggtgcggggc tccgatcgat gaacgccgca gctgcgatgg tcgccgacac
    10801 ccgcttgacg aggag
//
LOCUS       DASTNL010000157         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_108031, whole genome shotgun sequence.
ACCESSION   DASTNL010000157 DASTNL010000000
VERSION     DASTNL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108031"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..673)
                     /gene="rplO"
                     /locus_tag="VFQ96_04885"
     CDS             complement(191..673)
                     /gene="rplO"
                     /locus_tag="VFQ96_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU0257158.1"
                     /translation="MADDEKDGAAPGETRYQLLKVHHLRPAAGSRKNRIRVGRGEGSK
                     GKTSGRGTKGTKARYSVRPGFQGGQLPFHMRAPKLRGFKNAFRVEYQVVNLDRLAELY
                     PQGGEVTIADLVAKGAVRKNEKVKVLGGGEIAVALTVTVDKVSASAQEKILAAGGAIR
                     "
     gene            complement(673..858)
                     /gene="rpmD"
                     /locus_tag="VFQ96_04890"
     CDS             complement(673..858)
                     /gene="rpmD"
                     /locus_tag="VFQ96_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU0257159.1"
                     /translation="MAKHLKVTQIKSKVSERQNQRDTLRSLGLKRIGDSVVREDTEQN
                     RGYIRAVAHLVDVEEMD"
     gene            complement(864..1598)
                     /gene="rpsE"
                     /locus_tag="VFQ96_04895"
     CDS             complement(864..1598)
                     /gene="rpsE"
                     /locus_tag="VFQ96_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU0257160.1"
                     /translation="MSTETDKEQNVADTNAAEGTAATQDAQHDSRDRGDRRGGGRERG
                     GRDRGGRGDRRGSGRGGRDERNQFLERVVTINRVSKVVKGGRRFSFTALVVVGDGDGT
                     VGVGYGKAREVPLAISKGVEEAKKNFFRVPRLEKTIPHPVQGEAAAGVVLLRPAAAGT
                     GVIAGGPVRAVLECAGIHDVLSKSLGSSNTINIVHATIAALHQLEEPRTVAARRGLDY
                     ENVAPARFLRAEARAARQAAEAKAGA"
     gene            complement(1595..1963)
                     /gene="rplR"
                     /locus_tag="VFQ96_04900"
     CDS             complement(1595..1963)
                     /gene="rplR"
                     /locus_tag="VFQ96_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU0257161.1"
                     /translation="MSNSRGTSRAAARGRRHTRLRKKVVGTQERPRMVVTRSSRHVFV
                     QVVDDGAGRTLASGSTMETGLRTFEGDKTAKARRVGELVAERAKAAGIAAVVFDRGGA
                     KYAGRVAAVADGAREGGLDL"
     gene            complement(1964..>2406)
                     /gene="rplF"
                     /locus_tag="VFQ96_04905"
     CDS             complement(1964..>2406)
                     /gene="rplF"
                     /locus_tag="VFQ96_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU0257162.1"
                     /translation="LRLTVAHPIEVAVQEGQVLVTRPDDERQSRALHGLTRTLIHNNI
                     VGVTQGYSKGLEIVGTGYRVAQKGSAVEFALGFSHPVTVEPPAGITLTVEGNTKLTVS
                     GIDKQAVGETAANIRKIRKPEPYKGKGIRYAGEVVRRKAGKAGK"
BASE COUNT          303 a          876 c          805 g          422 t
ORIGIN      
        1 caagcgtgaa cgcgatcttc ttgcgaagat ccggcgtctt gaagatccgc acgaaggcgc
       61 tgaacaaggg ggcatcctgc tttcgtctat ggcagtgggg ccgcgcagca cgcacatgcc
      121 gccgacccca ctttacttca cggggcgagc cccgcccgcg gccggacctc tcggcggccg
      181 cgggacgcga tcaccggatg gcgccgccgg cggccaggat cttctcctgg gccgaggcgg
      241 agaccttgtc gaccgtcacg gtcagggcga ccgcgatctc gcccccgccc agcaccttga
      301 ccttctcgtt cttgcgcacg gcgcccttgg ccacgaggtc ggcgatcgtc acctcaccgc
      361 cctgcgggta gagctcggcg agacggtcga ggttcacgac ctggtactcc acgcggaagg
      421 cgttcttgaa tccgcgcagc ttcggggcac gcatgtggaa cggcagctgg ccgccctgga
      481 agccgggacg taccgagtag cgcgctttgg tccccttggt gccgcggccg gaggtcttgc
      541 ccttggatcc ctcgccgcgg ccgacgcgga tcctgttctt gcgtgagcct gccgcagggc
      601 gcaggtgatg caccttgagc agctggtagc gggtctcgcc cggggcggcg ccgtccttct
      661 cgtcgtcagc catcagtcca tctcctcgac gtcgacgagg tgtgcgacgg cgcggatgta
      721 gccgcggttc tgctcggtgt cctcgcgcac gacgctgtcg ccgatgcgct tgaggccgag
      781 gctgcgcagc gtgtcacgct ggttctgccg ctcgctcacc tttgatttga tctgggtcac
      841 tttgaggtgt ttggccatga tcttcaggca cctgccttcg cttcggccgc ctgacgggcc
      901 gcacgggcct cggcgcgcag gaagcgggcc ggggcgacgt tctcgtagtc gagcccgcga
      961 cgggccgcga cggtgcgcgg ctcctcgagc tggtgcaggg ccgcgatcgt cgcgtgcacg
     1021 atgttgatcg tgttggagga gcccagggac ttgctcagca catcgtggat cccggcgcat
     1081 tcgagcacgg cgcgcaccgg gccaccggcg atgaccccgg tccccgccgc agccggcctc
     1141 agcagcacca cgccggccgc cgcctcgccc tgcacgggat gcgggatggt cttctcgagg
     1201 cgcgggacgc ggaagaagtt cttcttcgcc tcctcgacgc ccttcgagat cgccagcggg
     1261 acctcgcgcg ccttgccgta gccgaccccg accgtcccgt cgccgtcgcc gacgacgacg
     1321 agcgccgtga agctgaacct ccggccgccc ttgacgacct tcgagacgcg gttgatggtc
     1381 acgacacgct ccaggaactg gttgcgctca tcgcggccgc cacggccgct gccacggcga
     1441 tccccgcggc cgccgcggtc acgaccgcca cgctcccggc caccgccacg gcgatccccg
     1501 cggtccctcg agtcgtgctg ggcgtcctgc gtcgcggcag tgccctccgc ggcgttggtg
     1561 tcggccacgt tctgctcctt gtccgtttcc gtgctcacag gtccagcccc ccttcacgtg
     1621 caccgtcggc gacggccgcc acgcggcctg catacttcgc cccgcctcga tcgaagacca
     1681 cggcggcgat ccccgcggcc ttggcccgtt cggccacgag ctcgccgaca cggcgggcct
     1741 tggccgtctt gtcgccctcg aaggtgcgca gtccggtctc catcgtggac ccggaggcga
     1801 gcgtgcgccc ggccccgtcg tcgacgacct gcacgaagac gtgacgtgac gagcgcgtga
     1861 cgaccatgcg cggccgctcc tgcgtgccga cgaccttctt gcgcagccgc gtgtgccggc
     1921 gtccgcgtgc cgcggccctg ctggttcctc tgctgttgga cacttactta ccagccttcc
     1981 cggccttgcg acgcacgacc tcgcccgcgt aacggatgcc cttgcccttg tacggttccg
     2041 gcttgcggat cttgcggatg ttcgccgccg tctcgccgac agcctgcttg tcgatgccgc
     2101 tgaccgtgag cttcgtgttc ccctcgacgg tcagggtgat cccagccggc ggctcgacgg
     2161 tgaccgggtg ggagaagccg agcgcgaact cgaccgccga ccccttctgg gcgacgcggt
     2221 agcctgtgcc gacgatctcc aggcccttcg aatagccctg ggtgacgccg acgatgttgt
     2281 tgtggatcag cgtgcgggtc aacccgtgca atgcccggga ctgccgctcg tcgtcggggc
     2341 gggtgacgag gacctggccc tcctggaccg cgacctcgat cgggtgggcc acggtgagcc
     2401 ggagct
//
LOCUS       DASTNL010000158         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_292622, whole genome shotgun sequence.
ACCESSION   DASTNL010000158 DASTNL010000000
VERSION     DASTNL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292622"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..641)
                     /gene="ruvX"
                     /locus_tag="VFQ96_04910"
     CDS             complement(135..641)
                     /gene="ruvX"
                     /locus_tag="VFQ96_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU0257163.1"
                     /translation="MLRGVRLGVDVGTVRIGVSKSDPDGVLASPLDTVPRASDGADVR
                     LIADLATRYRAIGIVVGHPLALSGRTTRSTRDAVSFAERLAAATAVPVWLVDERLSTV
                     SAAQALHAAGKNSRRQRGVIDRSAAAVILQHALEAERQAGRPAGRAVGPSAEAGRDDA
                     PCEDDSDE"
     gene            complement(648..3308)
                     /gene="alaS"
                     /locus_tag="VFQ96_04915"
     CDS             complement(648..3308)
                     /gene="alaS"
                     /locus_tag="VFQ96_04915"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU0257164.1"
                     /translation="METAEIHRRWLAFFAERGHTVVPSASLVSDDPSLLFTVAGMVPF
                     VPYLTGLVPAPFPRATSVQKCIRTNDIDEVGKTPRHGTFFQMCGNFSFGDYFKEQAIS
                     FAWELLTTPESAGGLGFSPDDLWVTVYENDHEARQIWRRVSGLADERIQGLGMDSNYW
                     STGQPGPAGPCSEIFFDRGPAYGVDGGPATDDDRYVEIWNLVFMQYLRGDGTGKADFE
                     ILGDLPRKNIDTGMGLERVAFLKQGVENMYEIDQVRPVLDAAAAMAGVTYGARHDADV
                     RLRVVADHVRSSLMLMTDGVSPSNEGRGYILRRLLRRTVRSMRLLGVHESAFPTLFQA
                     SRDAMSGAYPEVAAEFERTSRIAFGEEETFLRTLESGTGILETAVSATAKRGGTELSG
                     DTAFLLHDTYGFPIDLTLEIAEESGLTVDRAAFDTLMAEQRGRAKADARAKKRARADL
                     SVYSPFRALGETVFTGYTELTTESTVLGIIVDGRPVPSAGPGQNAEVILAASALYAES
                     GGQDADSGRIVGPGYDLEVLDVQRPVKGLISHTVRVADGEVAVGAPATSVVDADYRRG
                     ARQAHSGTHVIHAALRHVLGSTAHQSGSYNKAGYLRLDFSWNHALSPQTRSEIEEISN
                     NAIRSNLEVTTRELPLDEAKAMGAMALFGEKYGKVVRMVDIGGPWSRELCAGTHVATS
                     AEIGMINLVGESSVGSSARRVEALVGLEAFHDLAVERALVTQLSASLKAPRADLVNRV
                     TELTARLKKAEKKIAAYEERALADRVDSLVGRAERHGAAVLLAAEVENIRSSDDLRHL
                     ALLARGRLGDAAAVVALAGVVDAHPVVVVAVNAAAREAGYEAGVLAQGAARRLGGGGG
                     GKGDIAQGGGTDASAIRSALDGVAIALEHR"
     gene            complement(3350..3544)
                     /locus_tag="VFQ96_04920"
     CDS             complement(3350..3544)
                     /locus_tag="VFQ96_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257165.1"
                     /translation="MRRLVWLLTGVFVGFGLAHEMNKTDRGRAFFEAVNDRARDFRAA
                     VGEGYRSRDTELRFGSSRAR"
     gene            complement(3672..4301)
                     /gene="rpsD"
                     /locus_tag="VFQ96_04925"
     CDS             complement(3672..4301)
                     /gene="rpsD"
                     /locus_tag="VFQ96_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0257166.1"
                     /translation="MSTKSRTHSKTRLSRALGVALTPKAARYMDRRPYGPGEHGRGKR
                     KTDSDYAVRLREKQRLRAQYGIREAQLKIAFNEARRQAGLTGENLVEILEMRLDALVL
                     RAGFARTTAQARQFVVHRHIMVDGQIVDRPSYRVTPGALIHVKPRSEATEPFQVAAAG
                     GHAEVLPAVPGYLDVELDRLRAKLVRRPKRAEVPITCDVQLVVEYYAAR"
     gene            complement(4489..>5298)
                     /locus_tag="VFQ96_04930"
     CDS             complement(4489..>5298)
                     /locus_tag="VFQ96_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU0257167.1"
                     /translation="AIIRLATGDARRALTVLEAASAAAGEAESEAAGTVGRPDDDEAE
                     EAGAAGETAVRPRIVTAELVARTLDRALVRYDKTGDQHYDVTSAFIKSLRGSDVDAAL
                     HYLARMIEAGEDPRFIARRLVILASEDVGMADPQALRVAVAAAQAVQLVGMPEGRIPL
                     AQAVIHLAMAPKSNSAYLAIDRAIADVRAGTIGQVPAPLRDSHYAGAAGLGHGAGYRY
                     PHDDPRGVVEQQYLPKRLRGAVYYRPSEHGAEREVALRLPKIRRVVRGEEP"
BASE COUNT          753 a         1867 c         1843 g          835 t
ORIGIN      
        1 gttgttgcgg cggcgtgaga cgcggagcgg gcggggcggt atgacggccg ctcggccgcc
       61 gcccggccgg ccggcggtgc ccgtcgccgc cgcggcgggc gggtccgacc ccggcgctta
      121 ggcccgatcc gccctcactc atcgctgtcg tcctcgcagg gggcgtcatc acggcccgcc
      181 tctgccgaag gccccacggc ccggcctgcc ggcctgcccg cctgccgctc agcctccaag
      241 gcgtgctgca agatgaccgc cgccgcgctg cggtcgatga cgccgcgctg ccgacggctg
      301 ttcttgccgg ccgcgtgtaa ggcctgcgcc gcgctcaccg tgctgagcct ttcgtcgacg
      361 agccacaccg gcaccgccgt cgctgcggcg agacgctcgg cgaagctgac cgcgtcgcgg
      421 gtggaacggg ttgtgcgacc cgagagtgcg agaggatgcc cgacgacgat gccgatggcg
      481 cggtaccggg tggcgaggtc ggcgatcagc cgcacgtctg cgccgtcgct cgcccgcggt
      541 accgtgtcga gcggcgaggc gaggacgccg tcggggtcgc tcttgctgac gccgatccgg
      601 accgttccga cgtcgactcc caagcgcacc ccgcgtagca tcatcgctca cctatgttcg
      661 agcgcgatcg ccaccccgtc aagggccgac cggatcgcgg acgcgtcggt gccgccgccc
      721 tgggcgatgt cgcccttgcc gccgccgccg ccgccgagcc tgcgcgccgc accctgtgcg
      781 agcacgcccg cctcgtatcc ggcctcgcgt gccgcggcgt tgaccgcgac gacgacgacc
      841 gggtgggcgt ccaccacgcc ggccaaggcg acgaccgccg cggcgtcgcc gagccggccc
      901 cgggcgagga gggcgagatg gcgcaggtcg tcggaggagc ggatattctc gacctcggcc
      961 gccaggagta cggcggcgcc gtgccgctcg gctcggccta caagggagtc gacccggtcg
     1021 gcaagagcgc gttcctcgta agccgcgatc ttcttctcgg ccttcttcaa ccgggcggtg
     1081 agctccgtga cgcggttcac gagatcggcg cgcggggcct tcagcgaggc ggagagctgc
     1141 gtcacgagcg cgcgttccac cgcgaggtcg tggaaggcct ccagcccgac gagcgcctcg
     1201 acgcgtcggg ccgacgagcc caccgacgat tcgccgacaa ggttgatcat gccgatctcg
     1261 gcgctcgtcg cgacgtgggt ccccgcgcac aactccctcg accagggacc gccgatgtcg
     1321 accatccgca cgaccttgcc gtatttctcg ccgaagagag ccatggcacc catcgccttg
     1381 gcctcatcaa gcggaagctc tcgggtcgtc acctcgaggt tgctgcggat cgcgttgttc
     1441 gagatctcct cgatctcgct gcgagtctgg ggggagagag cgtggttcca cgagaagtcg
     1501 agccgaagat aacctgcctt gttgtacgag cccgactgat gtgcggtgct tcccagcaca
     1561 tgccggagcg ccgcgtggat gacgtgcgtg ccggaatgcg cctggcgggc accccgccgg
     1621 tagtcggcgt cgacgacgct cgtcgcgggt gcccccaccg cgacctctcc gtcggccacc
     1681 cgcaccgtgt ggctgatgag gcccttgacc ggtcgctgca cgtcgaggac ctcgaggtcg
     1741 tacccgggtc ccacgatccg gccgctgtcc gcgtcttgcc cgcccgactc ggcgtagagc
     1801 gcgctggcgg cgaggatcac ctcggcgttc tggccgggcc ctgcggaggg cacggggcga
     1861 ccgtcgacga tgatgccgag caccgtggat tccgtggtga gctcggtgta cccggtgaag
     1921 acggtctcac cgagggcgcg gaacgggctg tacaccgaca ggtcggcgcg ggcccgtttc
     1981 ttagcgcgcg cgtcggcctt ggcccggccg cgctgctcgg ccatcagggt gtcgaacgcg
     2041 gcccggtcga ccgtcagacc cgactcttcg gcgatctcga gagtgaggtc gatcgggaag
     2101 ccgtacgtgt cgtgaaggag gaaggcagtg tcgccagaaa gttcggtgcc gcctcgtttg
     2161 gccgtcgcgc tcaccgcggt ctcgaggatc ccggtgcccg actcgagcgt gcgcaagaag
     2221 gtctcttctt cgccgaacgc gatgcgtgac gtgcgttcga actctgccgc cacctcgggg
     2281 taggcgccac tcatcgcgtc acgtgaggcc tggaacagcg tcgggaaggc cgactcgtga
     2341 acgccgagca ggcgcatgct gcgcacggtg cgccgcaaca ggcggcgcaa gatgtagccg
     2401 cggccctcgt tcgacgggct gaccccgtcg gtcatgagca tgagcgagct gcgcacgtgg
     2461 tcggcgacga cgcgcaggcg cacgtcggcg tcgtggcggg ctccgtaggt gacaccggcc
     2521 atcgccgccg cggcgtcgag caccgggcgt acctggtcga tctcgtacat gttttcgacg
     2581 ccttgcttca ggaaggcgac gcgttccaga cccatgccgg tgtcgatgtt cttgcgtggc
     2641 aggtcgccga gaatctcaaa gtcggccttg ccggtgccgt cgccccgcag gtattgcatg
     2701 aacacgaggt tccagatctc gacgtagcgg tcgtcgtcgg tggcaggccc tccgtcgacg
     2761 ccgtaggccg ggccacggtc gaagaagatc tccgagcacg ggccggccgg gccgggttgg
     2821 cccgtcgacc agtagttgct gtccatgccg aggccctgga tgcgttcgtc tgcgaggccg
     2881 gagacgcggc gccagatctg acgtgcctcg tggtcgttct cgtagaccgt gacccacagg
     2941 tcgtcgggcg agaagccgag gccgccggcg gactccggcg tggtcagaag ctcccacgcg
     3001 aagctgatcg cctgctcttt gaagtagtcg ccgaacgaga agttgccgca catctggaag
     3061 aacgtgccat gccgcggggt cttgccgacc tcgtcgatgt cgttcgtgcg aatgcacttc
     3121 tgcacgctcg tcgcccgcgg gaagggcgcc ggcaccagtc cggtcagata gggcacgaac
     3181 ggaaccatgc cggccaccgt gaagagaagc gaaggatcgt cgctgaccag cgacgcagac
     3241 ggcacgaccg tgtgcccccg ctccgcgaag aaggcgagcc aacgccggtg gatctcagca
     3301 gtctccatat ggtccattca tccgttcgtg tctgccttcc ggccgagtct catcgagctc
     3361 gagacgaacc gaagcgcaac tcggtgtcgc gcgagcggta gccctcgccg accgcggcgc
     3421 gaaagtcgcg ggcccggtcg ttgaccgctt cgaagaacgc gcgaccgcgg tcggtcttgt
     3481 tcatctcgtg cgccaacccg aagcccacga agacgcccgt gaggagccac accagtcgtc
     3541 tcatctctgc cacgctctct ctttcagacg gcgccgcctc gcgcgctcac ccgccagttt
     3601 atcggcgccc gccggccgaa aacggcggtg ggccgcttcg cgcggcccac cgtgacgatc
     3661 cgagtctcgc cctagcgggc ggcgtagtac tcgacgacga gttgcacgtc acaggtgatg
     3721 ggcacctcgg cacgcttggg gcggcgcacg agtttggccc gcaaccggtc gagctccacg
     3781 tcgaggtagc ccggcacggc cggcagcacc tcggcgtgac cacccgcggc cgcgacctgg
     3841 aatggctcgg tcgcctcgct gcgcggcttg acgtgaatca gcgctcccgg cgtcacacgg
     3901 tacgacgggc gatcgacgat ctggccatcg accatgatgt gccggtgcac gacgaactgc
     3961 cgcgcctggg ctgtggtgcg ggcgaagccg gcgcgcagca ccagggcgtc gaggcgcatc
     4021 tccagaatct cgaccaggtt ctcaccggtc aaacctgcct ggcgccgggc ctcgttgaac
     4081 gcgatcttca gctgagcctc gcggatgccg tactgcgcgc gcagacgctg cttctcgcgc
     4141 agccgcaccg cgtagtcgga gtcggtcttg cgcttgccgc ggccgtgttc acccggcccg
     4201 tagggcctgc ggtccatgta gcgcgccgcc ttcggcgtca gcgccacacc cagggctcgg
     4261 gagagccggg tcttgctgtg tgtccgtgac ttggtggaca agtcgttcct tccaatcggt
     4321 ctgtgttctc cgcggcgcgt acacgcgcgg catgcgtgat gagaggggtc tcgcccgggt
     4381 gcccggctac agggccaacc cttcaaacgg tggcgtgcag ccgcacaccc cacggttcag
     4441 aggcaggcta atagtaccag ccgcgtgggg cgcggcggcc gcccgggttc acggttcctc
     4501 cccgcggacg acccgtcgaa tcttcggcag gcgcagagcg acctcgcgtt cggccccgtg
     4561 ttcgctcggc cggtagtaga cggcgccgcg cagccgcttc ggcaggtact gctgctcgac
     4621 cacgccccgc ggatcgtcgt gtgggtaacg gtagccggcg ccgtggccga gacctgccgc
     4681 gcccgcatag tgcgagtcgc gcagcggggc gggaacctgg ccgatcgtgc cggcgcgcac
     4741 gtcggcgatc gcccggtcta tcgcgagata ggcggagttc gatttgggcg ccatcgccag
     4801 atggatgacg gcctgcgcca gcgggatgcg cccttccggc atgccgacga gctgcacggc
     4861 ctgcgcggcg gcgacggcca cccggagcgc ctgcggatcg gccatgccga cgtcttcgct
     4921 cgccaggatc acgagccgcc tggcgatgaa gcgcggatct tcccccgcct cgatcatccg
     4981 cgccaaataa tgcaacgcgg cgtcgacgtc ggagccccgc agcgacttga tgaacgcgct
     5041 cgtgacgtcg tagtgctggt cacccgtctt gtcgtagcgc acgagggcac ggtcgagggt
     5101 gcgcgcgacg agctcggccg tcacgatgcg aggccgcaca gcggtctcgc ccgccgcccc
     5161 ggcctcctcc gcctcgtcgt cgtcgggccg gccgacggta ccggcggcct cgctctcggc
     5221 ctcgcctgcc gcggccgagg ccgcctccag caccgtgagt gcccgacgcg cgtcgcccgt
     5281 tgccagcctg atgatggc
//
LOCUS       DASTNL010000159         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_108733, whole genome shotgun sequence.
ACCESSION   DASTNL010000159 DASTNL010000000
VERSION     DASTNL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108733"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1086)
                     /locus_tag="VFQ96_04935"
     CDS             complement(196..1086)
                     /locus_tag="VFQ96_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0257168.1"
                     /translation="MGERDVVRRRAPEPIDLHTHSSVSDGTQAPAELVAAARRAGLGT
                     IALTDHDTTAGWEEARAAAARLHVAVVGGIELSTGYEGRSVHMLGYLVDPENPGLVAE
                     TARIRASRLARARRIVALIGRDYDFTWADVLAHVRSGATVGRPHIADALVARGYVPDR
                     SAAFAGILRAGRGYTLPHYAPDVVTGVRLIRAAGGVPVLAHPATGAGRLVVPEDELGT
                     LVEAGLFGLEVEHRLNTADGKRLLRRLAVRHGLVATGSSDYHGAGKPNLLGENRTPAG
                     VLDAIVREATGSEPFLPASL"
     gene            1147..>2384
                     /locus_tag="VFQ96_04940"
     CDS             1147..>2384
                     /locus_tag="VFQ96_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEU0257169.1"
                     /translation="MATIEKETARRSEKEASRRVPNRSTTPVSDRFRAYIAEGWAPRV
                     EAEAKPREQASFAAKRRERLSARYPGRRLIVPAGGRKRRSNDTDYPFRAHAAFAYLTG
                     WGADSEPGSVLVFEPTATGHETTLYFRERAGRDSDEFYANPEIGEFWIGPRPSLRHVA
                     GDLALPTRPLAEADQIVAGADETTLIVREADQALTARVDAARGGEDDARTAADAELAR
                     ELSEMRLVKDSYEVARVREAIAATADGFDDVIRALPRAVAHRRGERLVEGVFNTRARA
                     EGNAVGYDTIAAAGPHACILHWTRNDGAVRPGELILIDAGVEGDDYYTADITRTLPVG
                     GTFSATQRLVYEAVREAADAAFRIVRPGIRFRDVHATAMAVIARRTAEWGMLPVDARE
                     AQKPENQQYRRYMVHGTSHH"
BASE COUNT          362 a          854 c          869 g          299 t
ORIGIN      
        1 cagcatgcgg ctgccacacg cgcggcgggc gccgcacccg ttgcagaagg cgcgcacgac
       61 ggccaagggc gtgcccatca cgacggcaac agcgcccggc gccgcacccg gcgcggcggg
      121 ccggccggcg gcgctcccgc gccctcgacg gtcggctgag gcaccccgcc agttaagccg
      181 gcggggtgtg cccgctcaaa gggaggccgg caggaacggc tcgctgccgg tcgcctcacg
      241 cacgatggcg tctaacacgc cggctggcgt gcggttctca ccgagaaggt tcggcttgcc
      301 ggcgccgtgg tagtcgctcg agccggtagc gaccagcccg tgtcgcacgg cgaggcgccg
      361 cagcaggcgt ttgccgtcgg ccgtgttcag ccgatgctcg acctcgagcc cgaaaaggcc
      421 cgcctcgacg agtgtgccca actcatcctc ggggacgacc agccgccccg ccccggtcgc
      481 cgggtgtgcg aggacgggga ccccgccggc cgcgcggatc aggcgcacac cggtcacgac
      541 gtcgggtgcg tagtgcggca gagtgtagcc tcgcccggca cgcaggatgc cggcgaacgc
      601 cgcgctgcga tcgggcacgt agccgcgggc gacgagcgcg tcggcgatgt gagggcgacc
      661 gaccgtcgcg ccgctacgca cgtgggcgag aacatccgcc cacgtgaagt cgtagtcgcg
      721 cccgatgagg gcgacgatgc gtcgagcgcg ggccaggcgt gatgcgcgga tccgcgccgt
      781 ctcggcgacg aggccgggat tctccgggtc gacgaggtac cccagcatgt gcacgctgcg
      841 tccctcgtac ccggtggaga gctcgatgcc gccgacgacg gcgacgtgca gccgggcggc
      901 ggccgcgcga gcctcctccc agccggcggt ggtgtcgtgg tcggtcaacg cgatcgtgcc
      961 gaggcccgcg cgccgggccg cggccaccag ctccgccggc gcctgcgtgc cgtcggaaac
     1021 gctggaatgg gtgtggaggt cgatcggctc cggtgcccgc cttctgacca catcccgctc
     1081 gcccacaccc ccatcgtaag ggcgcgggca cggcccaccc ggcgccgccg gtgcgagact
     1141 ggaggcatgg cgacgatcga gaaagagact gcccggcgaa gcgagaaaga ggcatcgcgt
     1201 cgcgtgccga atcgttcgac gacccccgtc tcggatcggt tccgcgcgta catcgccgag
     1261 ggctgggcgc cccgcgtcga ggcggaggcg aagcctcgcg aacaggcatc gttcgccgcg
     1321 aagcggcgcg agcgcctctc ggcccgctac ccggggcggc gtctcatcgt tccggcgggc
     1381 gggcgcaaga gacgctcgaa cgacacggac tacccgttcc gtgcgcatgc tgcattcgcc
     1441 tatctcaccg gctggggcgc cgacagcgag ccaggcagcg tcctcgtctt cgagccgacc
     1501 gcgacaggcc atgagacgac cctttacttt cgtgagcgcg ccggacgaga ctccgacgag
     1561 ttctacgcca acccggagat cggcgagttc tggattggcc cgcgcccgtc gctcaggcac
     1621 gtcgccggcg acctggcgct tccgacccgg ccgttggccg aggccgacca gatcgtggcc
     1681 ggggcagacg agacgacgct catcgtgcgg gaggccgatc aggcactgac tgcccgggtg
     1741 gacgcggcac gcggcggcga agacgacgcc cgcacggcgg cggacgccga gttggcacgc
     1801 gagctctccg agatgcggct cgtgaaggat tcttacgagg tggcccgtgt gcgggaggcg
     1861 atagccgcca cggccgacgg cttcgacgac gtgatccgcg ccctgccgcg tgccgtcgcc
     1921 caccgccgcg gcgaacgcct ggtcgagggc gtcttcaaca cgcgggcccg cgccgagggc
     1981 aacgcggtgg gctacgacac gatcgcggcc gccgggccgc acgcctgcat cctgcattgg
     2041 acgcgcaacg acggtgcggt acggccgggc gagctgatcc tcatcgacgc cggcgtggag
     2101 ggcgacgatt actacaccgc cgacatcacc cggacgctcc ccgtgggagg cacgttctcg
     2161 gcgacgcagc gtctcgtcta cgaggcggtg cgagaggcgg cagacgccgc cttccgcatt
     2221 gtacggccag gcatccgttt ccgcgacgta cacgcgactg cgatggccgt gatcgccaga
     2281 cgcaccgcgg aatggggcat gctgccggtc gacgcccggg aggcccagaa gcccgagaac
     2341 cagcagtaca ggcgctatat ggtgcacggc acgagccacc actt
//
LOCUS       DASTNL010000160         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_108872, whole genome shotgun sequence.
ACCESSION   DASTNL010000160 DASTNL010000000
VERSION     DASTNL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108872"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="murF"
                     /locus_tag="VFQ96_04945"
     CDS             complement(<1..1202)
                     /gene="murF"
                     /locus_tag="VFQ96_04945"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU0257170.1"
                     /translation="MIRMSLAEIAQAVGGRLHALPHGVDPRSSVDGAVQTDSREITPG
                     GIFFAKRGEVTDGHLFAAAAARQGAALLIVEHPVEVALPQILVSDTVVALGALAHRVV
                     EAVRSRGRLKVVAVTGSNGKTTTKNLLAQILARTGTVVAPQASFNNSVGAPLTALRIT
                     EDTDFLVAEMGASHIGDISRLVAMEHPDVGIVLKVGMAHAGEFGGIEQTQKAKSEMVT
                     DLTPADVAVLNADDPRVAAMADLTRASVRWFGRDSAAQTRAVDVTSGIDGSRFTLDLS
                     DGRRAPVHLHVLGEHHVMNAVAAAAACDALGTPLDDIVAGIQSITRAERWRMEVLGGG
                     RGVTIINDAYNASPESMAAALKTLARVTPPEGRSVAVLGMMNELGEYSDEEHDRIGLL
                     AVRLGIDKV"
     gene            complement(1199..2746)
                     /locus_tag="VFQ96_04950"
     CDS             complement(1199..2746)
                     /locus_tag="VFQ96_04950"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU0257171.1"
                     /translation="MNATVAGDLRPEHPAGRPLTALASEFAFPARGLRSGVETTGVSI
                     NTGTLRPGDLYVAAAGRHSHGARYLSEARAAGAVGVLTDPPNADAAASLGFPVLLTPD
                     PRAAVGDVAAWIYRTNDFPLTKYAVTGTNGKTSVVYLLSALLTQLGVANGLSSTAERR
                     AGRGRATSRLTTPEAGELHALLARMREEAARAAAIEVSAQALTEHRVDGIVFDVAGFT
                     NLGHDHLDEYGDLETYFAAKRALFEPEHSRRAVVVIDTEWGRRIADDARIPIATLSAS
                     PGSEAAWHVAVTDESLRGVAILLTGPGGSTLATSIPIPGAHMGVNAALAIAMLVESGT
                     SLDAIGHVLARDGGIRVQVPGRLERVSGPHGPVFFIDYAHTPEAYRTTLADLRRVTTG
                     KLITVLGGSGDRDLAKRPINGAETARGSDIVIVTDQDPRSEDPAVIRAAVIDGARQAR
                     PGVELYEVPDVPQAVRKAISLATARDVILFATPAHSDHREVNGRHVPYSAYDDSRAAL
                     REAGWPA"
     gene            complement(2766..>3261)
                     /locus_tag="VFQ96_04955"
     CDS             complement(2766..>3261)
                     /locus_tag="VFQ96_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0257172.1"
                     /translation="SASDIQIDSVYQTLANGGVRLPASIIKGCRLPDGTMIDVPKGKP
                     VRVVKTSTAKEVVQMMQAVVDEGELSSELKIPGYHIGAKTGTAQQPNGSGGYKSTYVV
                     QVTGVAPIKDPQYVVSVVIGNPTKIKTSAAAAPLFKTIMSQVLKTYRVPPSTTPSPDY
                     PVYY"
BASE COUNT          456 a         1093 c         1155 g          557 t
ORIGIN      
        1 accttgtcga tccccaggcg caccgccaga aggccgatgc ggtcgtgctc ctcatcggaa
       61 tattcgccca gctcgttcat cattccgagc acggcgaccg agcgaccttc cggcggggtg
      121 acgcgtgcga gggtcttgag agccgccgcc atggattcgg ggctggcgtt gtaggcatcg
      181 ttgatgatgg tcacgccgcg gccgccgccg agcacctcca tgcgccaccg ttcggcacgg
      241 gtgatcgatt ggatgccggc gacgatgtca tcgagcggtg tgcccagggc atcgcacgcc
      301 gccgcggccg ccaccgcgtt catcacgtgg tgctcgccga ggacatggag atgcaccggg
      361 gcacggcgtc cgtcggagag gtcgagcgtg aaacggcttc cgtcgatgcc gctcgtgacg
      421 tcgaccgcgc gcgtctgggc tgcgctgtcg cgaccgaacc agcgaacgct tgcccgcgtc
      481 aggtcggcca tggcggcgac gcgagggtcg tcggcgttca agaccgcgac gtctgccggc
      541 gtcaggtcgg tgaccatctc gctcttggct ttctgcgtct gctcgatccc gccgaactcg
      601 ccggcgtgcg ccatgcccac cttgaggacg atgccgacgt cggggtgctc catggcgacc
      661 aggcgagaga tgtcgccgat gtgactggca cccatctccg cgacaagaaa atcggtgtct
      721 tcggtgatgc gcagcgcggt cagcggggcg ccgacggagt tgttgaagga tgcctgcggg
      781 gcgacgaccg tccccgtgcg ggcgaggatc tgtgccagca ggttcttcgt cgtcgtcttg
      841 ccgttggacc ccgtcacggc gacgaccttg agccgcccgc gggaccggac ggcctcgacg
      901 acccgatgcg ccagggcacc caaggcgacc acggtgtcag agacgaggat ctgcggcagc
      961 gcgacctcga ccgggtgctc gacgatgagg agggccgcgc cctggcgtgc cgcggccgcc
     1021 gcgaacaggt ggccgtcggt cacctcgccg cgcttggcga agaagatccc gcccggcgtg
     1081 atctcgcgtg agtctgtctg caccgcgccg tcgacgctcg agcgagggtc gacaccgtgc
     1141 ggcagggcgt ggagccgccc gcccaccgcc tgcgcaatct cggcgaggga catccgtatc
     1201 aagccggcca ccccgcctcg cgcaatgccg cacggctgtc gtcgtaagcc gagtacggca
     1261 cgtgccggcc gttcacctct ctgtggtcgc tatgggccgg cgtggcgaac aggatgacgt
     1321 cgcgggccgt cgccaaggag atcgccttgc ggacggcctg cggaacatcc ggcacctcat
     1381 acaactcgac accgggccgt gcctggcggg cgccgtcgat gaccgcggcc cggatcacgg
     1441 cggggtcttc gctgcgcggg tcttggtcgg tgacgatgac gatgtcgctg ccgcgcgccg
     1501 tctctgcgcc gttgatcggg cgcttcgcga gatcccggtc gccggagccg cctagaaccg
     1561 tgatgagctt tcctgtcgtg acacgtcgca ggtcggcgag cgtcgtgcgg tacgcctccg
     1621 gcgtgtgcgc gtagtcgatg aagaacaccg gcccgtgcgg cccggagacc cgttccaggc
     1681 gccccggcac ctgcacgcgg atcccgccgt ccctggccag gacgtggccg atcgcgtcga
     1741 ggctcgttcc cgattcgacg agcatggcga tcgccagtgc cgcattcacc cccatgtggg
     1801 ctccgggtat cggaatggat gtcgcaaggg tcgatccgcc cgggcccgtc aagaggatgg
     1861 cgacgccgcg caaggattcg tcggtgacgg cgacgtgcca cgccgcctcg ctcccgggcg
     1921 aggcgctgag cgtcgcgatc gggatgcgcg cgtcgtcggc gattctgcga ccccattcgg
     1981 tgtcgatcac gacgacggcg cggcgggagt gttccggctc gaagagtgcg cgtttcgcgg
     2041 cgaagtacgt ttccaggtcg ccgtactcgt cgagatggtc gtggccgagg ttcgtgaagc
     2101 cggccacgtc gaagacgatg ccgtcgaccc ggtgctctgt cagggcctgt gccgagacct
     2161 cgatcgcggc ggcgcgggcc gcttcttcgc gcatcctcgc caacaaggcg tgcaattcgc
     2221 cggcctcggg ggtcgtcagc cggctggtcg cccgcccgcg gccggcccgg cgttcggcgg
     2281 tggagctcaa tccgttggcc accccgagtt gcgtgagcag cgcagataag aggtagacga
     2341 cgctcgtctt gccgttggtg cccgtcacgg cgtatttcgt gagggggaag tcgttcgtgc
     2401 ggtagatcca cgccgcgacg tctcccaccg cggcccgtgg atcgggcgtg aggagcacgg
     2461 ggaacccgag gctcgcggcg gcgtcggcat tcggcgggtc ggtgagcacg ccgaccgctc
     2521 cggccgcccg ggcctcggag aggtagcgtg cgccgtgact gtgcctgccc gcggcggcga
     2581 cgtagaggtc tcctggccgg agagtcccgg tgttgatgct cacgccggtc gtctccaccc
     2641 cgctccgcag cccgcgggcg gggaaggcga attcggatgc caacgcggtc agcggcctgc
     2701 ctgcgggatg ctccgggcgc aggtcacctg ccaccgtcgc gttcactccg actcgcttcc
     2761 tgccgtcagt agtacaccgg gtaatccggt gacggcgtag tagatggcgg cacgcggtac
     2821 gttttcagca cctgcgacat gatcgtcttg aacaaggggg ctgccgcggc agacgtcttt
     2881 attttagtgg gattcccgat gaccaccgaa acgacgtact gcgggtcctt gatcggtgcc
     2941 accccggtga cctggacgac gtaggtcgac ttgtagccgc ccgacccgtt cggctgctgg
     3001 gcggtgccgg tcttggcgcc gatgtggtag ccgggaatct tcagctcgct ggagagctcc
     3061 ccctcgtcga cgaccgcctg catcatctgc acgacctctt tggcggtgct cgtcttgacg
     3121 acgcgcaccg gcttgccctt gggcacgtcg atcatcgtgc cgtccggcag ccggcagccc
     3181 ttgatgatgc tcgccggcag gcgaacgccg ccgttggcga gggtctggta gacgctgtcg
     3241 atctggatgt cgctggccga g
//
LOCUS       DASTNL010000161        10764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1590951, whole genome shotgun sequence.
ACCESSION   DASTNL010000161 DASTNL010000000
VERSION     DASTNL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590951"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /gene="carA"
                     /locus_tag="VFQ96_04960"
     CDS             <1..1037
                     /gene="carA"
                     /locus_tag="VFQ96_04960"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU0257173.1"
                     /translation="DPSYAGQIVVMTAPHIGNTGMNDDDMESQRLWVAGFVVRDPARR
                     VSNYRATRSLDDDLVAGGIVGIQGVDTRALTRHIRAAGAMRAGIFSGEHRASAPQQQL
                     EEVRSSSPMAGRNLSEVVSTARRYTLPAAAPRVGRVAVLDLGVKRSSLEQLRARGFDV
                     DVFPAAATAEEVLAAHPDAVFYSNGPGDPAASESQVALLRAVLTARVPYFGICFGNQL
                     LGRALGLATYKLPFGHRGINQPVLDKATGRVEITAQNHGFAVEAPTDQVIDSPAGLGR
                     VVVSHVSLNDQVVEGLRCLDLRAFSVQYHPEAAAGPHDAAHLFDRFRDFVLAGGQARD
                     QTADGQEGSL"
     gene            1037..4318
                     /gene="carB"
                     /locus_tag="VFQ96_04965"
     CDS             1037..4318
                     /gene="carB"
                     /locus_tag="VFQ96_04965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU0257174.1"
                     /translation="MPKRDDIASVLVIGSGPIVIGQACEFDYSGTQACRVLREEGIRV
                     ILVNSNPATIMTDPDFADATYVEPITWQVIEKIIAKERPDAILPTLGGQTALNAATEL
                     HEHGILDKYGVELIGAQFAAIQKGENRQTFKDLVVALGGDVAKSYIVHDVEEAVGHAE
                     DLGYPLVVRPSFTLGGLGSGFAHTEEELRRIVGSGLHESPSHEVLLEESILGWKEYEL
                     ELMRDAADNTVVVCTIENVDPVGVHTGDSITVAPSLTLTDREYQKLRDLGIAIIRGVG
                     VDTGGCNIQFAVDPATGRTIVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYRLDE
                     IPNDITRVTPASFEPTLDYVVVKVPRFAFEKFPAADQSLTTTMKSVGEAMAIGRNYTS
                     ALQKALRSVEKRGSSFHWGAEPRDLAELLRAVATPSEHRMVDVQQALRKGASVEDIFA
                     ASALDPWFIDQVALINEVADEVAAASALDEATLRLAKDHGFSDAQIAQLRGAAEHDVR
                     GLRHILGIRPVYKTVDTCAGEFPAATPYHYSSYDRETEVSPSERRKVVILGSGPNRIG
                     QGIEFDYSCVHASFALHDAGYETIMINCNPETVSTDYDTSDRLYFEPLTLEDVLEVID
                     AESASGELVGVIVQLGGQTALGLANGLQQAGVPILGTTPEAIDQAEERGLFAGILAEA
                     GLAAPQNGTAASYAQAVSVAHRIGYPVLVRPSYVLGGRGMEIVYDDDALRGYFDRVAD
                     TAIVGPRHPVLVDRFLDDAIEIDVDALYDGDRLYLGGIMEHIEEAGIHSGDSSCTLPP
                     VTLGGTQIARIREATEKIARGIGVRGLVNVQFAIAAGVLYVLEANPRASRTVPFASKA
                     QGISLAKAAARIMAGDSVEALVAEGMLRPEDGSVVAPGQAIAVKEAVLPFKRFRTELG
                     KIVDSVLGPEMHSTGEVMGIDVNFPRAFAKSQTAAYGGLPASGSVFLSVSDRDKRSIV
                     LPALRLHQLGFTILATEGTASVLLRHGIETSVVRKVSEPGSGADIVELIDRGDVGIVV
                     NTPSGGRGRRDGYEIRAAAVANDIPLFTTIAEVAAAVASLEGDREVAVRSLQEYGAAR
                     RVAAR"
     gene            4390..5292
                     /gene="pyrF"
                     /locus_tag="VFQ96_04970"
     CDS             4390..5292
                     /gene="pyrF"
                     /locus_tag="VFQ96_04970"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU0257175.1"
                     /translation="MTGDEPQADRSFGARLAAVFAREGRLSVGIDPHPETLAVWGLDD
                     TAEGAKRLGLAVVEACAGVAGIVKPQVACFERFGSAGFRALEAVLAESRQAGLLTIAD
                     AKRGDIGSTMASYADAWLSPGSPLEADAVTLTPYVGTGALEPTMRRAASHGKGVFLLA
                     ATSNPEARALQGARTPEGASVAGWIVGDVRAFNRRHAGNSVGRQGARVGAAGVVLGAT
                     VRLAQAGIEVGPGVGGGEGGVMPVLAPGFGSQGARLTDLEAIYGALTPGVIAHESRSL
                     LNVGPRRLRDEIARHAALARVVDA"
     gene            5309..6208
                     /gene="gmk"
                     /locus_tag="VFQ96_04975"
     CDS             5309..6208
                     /gene="gmk"
                     /locus_tag="VFQ96_04975"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU0257176.1"
                     /translation="MPAVDRQAASRAAVAARRARAVVKNSVQAGQLRPLDVLRDARRQ
                     PEGTAGRLRVEEFLRSIRGIGPRRAADVLAELRISRSRRLGGLGRHQAARLGDWLTKR
                     LDPDQIDPSRLVVLAGPTAVGKGTVASYIHEHYPDVLLSVSATTRAPRPGERDGVDYF
                     FVDDARFDRMIEAGEFLEWATVHNSHRYGTPRGPVEAALAAGRSVLLEIDLHGARSVR
                     RAMPGATLVFLAPPSWKELVRRLVGRGTEGEAERARRLATARVELAAKDEFDHRVVND
                     SVPAAAAEVVKLMAVRDSAPQQL"
     gene            6224..6481
                     /gene="rpoZ"
                     /locus_tag="VFQ96_04980"
     CDS             6224..6481
                     /gene="rpoZ"
                     /locus_tag="VFQ96_04980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU0257177.1"
                     /translation="MATPIVGIIDPPIDDLLSRVESKYALVLFASKRARQINDYYSDL
                     HEGSLFDNVGPLVDSTVDDKPLSIALHEINENKLVMKPAGE"
     gene            6474..7724
                     /gene="coaBC"
                     /locus_tag="VFQ96_04985"
     CDS             6474..7724
                     /gene="coaBC"
                     /locus_tag="VFQ96_04985"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU0257178.1"
                     /translation="MSEPTGAPVRPHILVGITGGIAAYKSVQLVRTLCLAGCDVRVVM
                     TEAATRFVGRATFEAISGNPVYEGLYEGVAEVRHIALALWCDEIVIAPATADTLARLA
                     AGRADDLLTATALAGTSPVVVAPAMHTGMWENPATQANLATLRRRGVHVVGPEEGRLT
                     SGDVGVGRMSDPEAIAAAARALRVGGRAGRSGPLAGKRVVITAGGTREPLDPVRFLGN
                     RSTGRQGVLLAEEAARRGADVTLIAAHLEVAVPAGVRRVDVSTALDMRQSLRDTLERA
                     DVVVMCAAVADFRPAASAVTKIKKDSFDEKAGLRLVRNPDILAEVGALAERSFVLVGF
                     AAETEPDRTGLVARGRAKLAAKRCDFLVVNAVGWRSGFGAGEATAFLLSAADDIVTDI
                     SGPKARVAEGVLDAVASRLGGASA"
     gene            7797..9020
                     /gene="metK"
                     /locus_tag="VFQ96_04990"
     CDS             7797..9020
                     /gene="metK"
                     /locus_tag="VFQ96_04990"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU0257179.1"
                     /translation="MSQLRLFTSESVTEGHPDKLCDQISDSILDALLAADPQSRVAVE
                     TMVTTGLVHVAGEVTTRAYVEIPQIVRDRITSIGYDSSDVWFDGRSCGVSVSIGQQSP
                     DIARGVDTALEARGAADTPGGDESRGDSDPLDGQGAGDQGTMFGYATKETPSLMPMPI
                     WLAHRMAQRLAEVRREGILGYLRPDGKTQVTVGYDDGGVPRTVETVVVSAQHSPRVTT
                     DRLRSDIREVVIRPVLEGSGLDVASVRTLINPTGRFEIGGPHADAGLTGRKIIVDTYG
                     GSARHGGGAFSGKDPSKVDRSAAYALRWVAKNAVAAGLADRLEVQVAYAIGSARPVGL
                     YVESFGTGHVPDQRIIAAIRAVFDLRPAAIIRDLDLLRPIYAQTAAYGHFGRELADFT
                     WERTDRVERLRDAAF"
     gene            9025..>10764
                     /locus_tag="VFQ96_04995"
     CDS             9025..>10764
                     /locus_tag="VFQ96_04995"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU0257180.1"
                     /translation="MPPYAFVARIVLESLLPQLDHPFEYGVPARLAQQVTPGVRVRVP
                     LRRGSQHVTGFVVETAREPAHDGELSEVEGVVSVLPVLAPEVWKLARAVADRGAGTAA
                     DVLRLAIPARAARVEMSGPGEEPGAPLPQPPAAPGHPAGYFAGHLEGRSRLALTVPPG
                     VVRFPAGRPGGERWLRSWAPTLASAAVACLRRGESAILAVPDHRDTDEVEAALAGAGA
                     AAWTARIDATGSNTRRYRAFLSCLAGPRIVIGNRSALYAPATKLGLIAVWDDGDPIHA
                     EPRAPYAHARDVALLRQQQQSTALIFAAYARSTAVERLVRIGWLAAAGPERVTRPRVI
                     VTSRQADDGRGARIPSGAFQAAQEASRDGPVLVQVARPGYAPVLSCDTCREPARCPHC
                     HGPLGLAAPGAAPACRRCGRSVPGWSCPVCGGTRLRMIGVGASRTAEELGRAFPGLSV
                     VVADGEHPVDRIPATPALVIATRGAEPSAEGGYRAVLLLDAARMLRRESLWVAEDCLR
                     VWTNAAALAAPGAPVVLVGVAGPLADAFSTWRHERYAERELDDRALLRLPPAVRIATV
                     TGRPAALEAAREAL"
BASE COUNT         1550 a         3879 c         3802 g         1533 t
ORIGIN      
        1 ccgacccgag ctacgcgggc cagatcgtcg tcatgacggc gccgcacatc ggcaacacgg
       61 gcatgaacga cgacgacatg gagtcgcagc gcctctgggt cgcaggcttc gtcgtgcgcg
      121 acccggccag gcgggtctcc aattaccggg ccacgcgcag cctcgacgac gacctcgtcg
      181 ccggcgggat cgtcggcatc cagggcgtcg acacgcgggc actcacccgg cacatccgcg
      241 ccgcgggggc catgcgcgcc ggcatcttct ccggtgagca ccgggcgtcg gctccgcagc
      301 agcagctgga ggaggtgcgc tcctcctcgc cgatggcggg ccggaacctc tccgaggtcg
      361 tctccaccgc ccgccgttac acgcttcccg cggcagcgcc gcgcgtcggc cgggtcgcgg
      421 tgctcgacct cggcgtgaag cgttcctccc tggaacagct gcgcgcccgc ggcttcgacg
      481 tcgacgtctt cccggccgcg gcgacggccg aggaggtgct cgcggcccat cccgacgcgg
      541 tgttctactc gaacgggccg ggggatccgg ccgcgtcaga gtcgcaggtc gccttgctgc
      601 gggccgtcct caccgcccgc gtgccctatt tcggcatctg cttcggcaac cagctgctcg
      661 gccgggcgct cggcctggcc acctacaagc tgccgttcgg gcaccgtggc atcaaccagc
      721 cggtgctcga caaggcgacg ggccgggtcg agatcaccgc gcagaaccac gggttcgcgg
      781 tcgaggcgcc caccgaccag gtgatcgact ccccggccgg cctcggccgc gtcgtggtga
      841 gccacgtgag cctcaacgac caggtcgtcg aggggctgcg ctgcctcgac ctcagggcgt
      901 tcagtgttca ataccacccg gaggccgccg cggggcccca cgacgccgca cacctcttcg
      961 accggttccg cgacttcgtc ctggccggcg ggcaggcccg cgaccaaacc gccgacggcc
     1021 aggaaggatc cctctgatgc cgaaacgtga cgacatcgca agcgttctcg tcatcgggtc
     1081 gggccccatc gtcatcgggc aggcctgcga gttcgactac tccggcaccc aggcgtgccg
     1141 cgtcttgcgc gaggagggca tccgcgtcat cctcgtcaac tccaacccgg ccacgatcat
     1201 gaccgacccc gacttcgccg acgccaccta cgtggagccg atcacgtggc aggtgatcga
     1261 gaagatcatc gcgaaggagc ggcccgacgc gatcctgccg accctcggcg ggcagacggc
     1321 gctcaacgcc gccaccgagt tgcacgagca cggcattctc gacaaatacg gcgtggaact
     1381 catcggcgcg cagttcgcgg cgatccagaa gggcgagaac cggcagacct tcaaggatct
     1441 cgtcgtcgcg ctgggcggcg acgtcgcgaa gtcgtacatc gtgcacgacg tggaagaggc
     1501 cgtcggtcac gccgaagatc tcggctatcc gctcgtcgtg cgtccctctt tcaccctcgg
     1561 cgggctcggc tccggcttcg cccacaccga ggaggagctg cggcgtatcg tcggcagcgg
     1621 cctgcacgag agccccagcc atgaggtgct cctggaggag tcgattctcg gctggaagga
     1681 atacgagctc gagctgatgc gcgacgcggc cgacaacacc gtcgtcgtct gcacgatcga
     1741 gaacgtcgac ccggtgggcg ttcacacggg tgactccatc accgtcgcgc cgtcgctgac
     1801 gttgaccgac cgggaatacc agaagctgcg ggacctcggc atcgcgatca tccgcggggt
     1861 gggcgtcgac acgggcggct gcaacatcca gttcgcggtc gatccggcaa cgggccgcac
     1921 gatcgtgatc gagatgaacc cgcgggtctc ccgctccagc gcgctcgcct cgaaggccac
     1981 cggcttcccg atagcgaaga tcgcggcgaa gcttgccatc ggctatcggc tcgacgagat
     2041 ccccaacgac atcacccggg tgacaccggc ctccttcgag ccgaccctcg actacgtcgt
     2101 cgtcaaggtg ccgcggttcg cgttcgagaa gttccccgcg gccgaccaaa gcctcacgac
     2161 gacgatgaaa tcggtcggcg aggcgatggc gatcggccgc aactacacga gcgccctgca
     2221 gaaggcgctg cgctccgtcg agaagcgggg gtcctcgttc cattgggggg cggagccgcg
     2281 cgacctcgcc gagctgctcc gcgccgtcgc gaccccgagc gagcaccgca tggtcgacgt
     2341 gcagcaggcc ctgcgcaagg gcgccagcgt cgaggacatc ttcgcggcca gcgccctcga
     2401 tccgtggttc atcgaccagg tcgccctcat caacgaggtc gccgacgagg tggccgccgc
     2461 gtcggcgctc gacgaggcca cattgcgtct ggccaaagac cacggcttct ccgacgcaca
     2521 gatcgcccag ctgcgcggcg ccgccgagca cgacgtgcgc ggcctgcggc acatcctcgg
     2581 catccgcccg gtctacaaga ccgtcgacac ctgcgccggc gagttccccg ccgccacgcc
     2641 ctaccactat tccagctacg accgggagac cgaggtttcg ccgagcgagc gacgcaaggt
     2701 ggtgatcctc ggctccggcc cgaaccgcat cgggcagggc atcgagttcg actactcctg
     2761 cgtgcatgcc tcgttcgccc tgcacgacgc cgggtacgag accatcatga tcaactgcaa
     2821 cccagagacg gtctcgaccg actacgacac gagcgaccgc ctctacttcg agccgctcac
     2881 gttggaggac gtgctcgagg tgatcgacgc cgagtcggcc agcggcgagc tcgtcggcgt
     2941 gatcgtgcag ctgggcggcc agacagccct ggggctcgcg aacgggctgc agcaggccgg
     3001 tgtccccatt ctcggcacga cgccggaggc catcgatcag gcggaggagc gcgggctgtt
     3061 cgccggcatc ctcgcggagg cggggctcgc cgcaccgcag aacggcaccg cggcctcgta
     3121 cgcgcaggcc gtgagcgtcg cacaccgcat cggctacccg gtgctcgtgc gaccctccta
     3181 cgtgctgggc ggccggggta tggagatcgt ctacgacgac gacgcgttgc gcggctactt
     3241 cgaccgggtg gcagacacgg cgatcgtcgg accccggcac ccggtgctcg tcgaccgctt
     3301 cctcgacgac gcgatcgaga tcgacgtcga cgctctctac gacggcgacc ggctctacct
     3361 gggcgggatc atggagcata tcgaagaggc cggcatccat tccggcgatt cgagctgcac
     3421 cctgccgccg gtgacgctcg gcggcaccca gatcgcccgg atccgtgagg cgacggagaa
     3481 gatcgcccgc ggtatcggcg tgcgcggcct cgtgaacgtg cagttcgcga tcgccgccgg
     3541 cgtgctgtac gtcttggagg cgaacccacg ggcgtcccgc acggtaccgt tcgcgtcgaa
     3601 ggcgcagggc atctcgctcg ccaaggccgc cgcccgcatc atggcaggag actcggtcga
     3661 ggctctcgtc gccgaaggca tgctccgccc ggaggacggc tccgtcgtcg ccccgggcca
     3721 ggcgatcgcc gtgaaagagg cggtgctgcc gttcaagagg ttccgcaccg aactcggcaa
     3781 gatcgtcgac tccgtgctcg ggcccgagat gcactccacc ggcgaggtga tgggcatcga
     3841 cgtgaacttc ccgcgcgcat tcgcgaagag ccagaccgcg gcctacggcg ggctgcccgc
     3901 ctccggcagc gtcttcctct ctgtctccga ccgcgacaag cgttcgatcg tccttccggc
     3961 gctgcggttg caccagctgg gcttcacgat cctcgcgacg gagggcaccg cgagcgtgct
     4021 cctgcgtcac gggatcgaga cgagcgtggt acgcaaggtg agcgagccgg gctccggcgc
     4081 cgacatcgtc gagctgatcg atcggggaga cgtcggcatc gtcgtcaaca cgccgagcgg
     4141 cgggcgcggg cgccgcgacg gctacgagat ccgggccgcc gccgtcgcga acgacatacc
     4201 gctcttcacc acgatcgccg aggtggcggc cgcggtcgcc tccctcgagg gcgaccgcga
     4261 ggtggcggtg cgctcgctgc aagagtatgg ggcagcccgc cgggtggcgg cacggtgagc
     4321 ccggctgagg ctacggctgc ggtcccgcgg gccctgtgag cgggggcctg tcgtgaacgg
     4381 tgtgacgtcg tgaccggcga cgaaccccag gctgaccggt ccttcggggc gcgcctcgcc
     4441 gcggtcttcg cccgcgaggg ccgcctcagc gtcggcatcg acccgcatcc agagacgctc
     4501 gccgtctggg gcctcgacga cacggccgag ggcgcgaaac ggctgggcct ggccgtcgtc
     4561 gaggcgtgcg ccggcgtggc cggcatcgtg aagccgcagg tggcctgctt cgagcggttc
     4621 ggttccgccg gattccgcgc gctcgaggcg gtgctcgccg aatcccggca ggcgggcctc
     4681 ctgacgatcg ccgacgcgaa gcgcggcgac atcggcagca cgatggcctc gtatgccgac
     4741 gcctggctct cgcccggatc cccgctggag gccgacgcag tgacgttgac accctacgtg
     4801 ggcaccggtg ccctcgaacc gacgatgcgg cgggcagcct cgcacggcaa gggagtcttc
     4861 ctcctcgccg ccacctcgaa cccggaggcg cgggcgttgc agggggctcg cactccggag
     4921 ggcgcgagcg tggccggctg gatcgtcggc gacgtgcgcg ccttcaaccg gcgccacgcc
     4981 ggcaattcgg ttggccggca aggcgcgaga gtcggcgcgg ccggtgtggt cctcggcgca
     5041 acggtacgct tggcccaggc cggcatcgag gtcggccccg gcgtcggggg aggcgaggga
     5101 ggcgtgatgc ccgtgctcgc acccggcttc ggcagccagg gagcccgcct caccgacctc
     5161 gaggccatct acggcgcgtt gacgcccggc gtcatcgccc acgagtcccg ttccctgttg
     5221 aacgtcgggc cccgccggct ccgcgacgag atcgcgcggc acgccgccct ggcacgggtt
     5281 gtcgatgcct gagacccgtc ctcgcgtcat gcctgccgtt gatcggcagg cggcctcgcg
     5341 tgccgccgtc gccgctcggc gggcgagggc cgtggtcaag aacagcgtgc aggccgggca
     5401 gttgcggccg ctcgacgtgc tccgtgacgc ccgccgccag ccggagggca cggccgggcg
     5461 gctgcgcgtc gaagagttct tgcggagcat ccgcggcata ggcccgcgcc gcgccgccga
     5521 cgtcctcgcc gaactgcgca tctcccgcag ccgccggctc ggcgggctcg gccggcatca
     5581 ggcggcccgg ctgggcgact ggttgacgaa gcgtctcgac ccggatcaga tcgacccgtc
     5641 gagactcgtg gtgctcgcgg gccccaccgc ggtcggcaaa ggcacggtcg cctcctacat
     5701 ccacgagcac tatcccgacg tgctgctctc cgtctcggcc accacgaggg cgccgaggcc
     5761 gggcgagcgg gacggggtcg attacttctt cgtggacgac gcccgattcg atcgcatgat
     5821 tgaggcaggc gagtttctcg aatgggctac cgtgcacaac tctcaccggt atggcacgcc
     5881 gcgcgggccg gtcgaggcgg cactggccgc cgggcgaagc gtgctgctcg agatcgacct
     5941 gcacggcgcc cgcagcgttc gccgagcgat gcccggggcg acgcttgtct tcctcgcccc
     6001 gccgagctgg aaggaactgg tgcgccggct ggtcgggcgg ggcaccgagg gagaggccga
     6061 acgcgcccgg cgactggcga cggcccgcgt cgagctcgcg gcgaaagacg agttcgatca
     6121 ccgggtagtc aacgactccg tgcccgccgc cgccgcggaa gtcgtaaagt tgatggcagt
     6181 gcgtgatagc gcaccgcagc agctttaagg agtcccccct cgcatggcca caccgatcgt
     6241 cggtatcatc gacccgccca ttgacgacct gctcagtcgc gtcgagtcca agtacgcgct
     6301 cgtcctcttc gcgtcgaagc gggcgcgtca gatcaacgac tactactccg acctgcacga
     6361 gggcagcctc ttcgacaacg tcgggccgct cgtggacagc acggtggacg acaagccgtt
     6421 gtccatcgcg ttgcacgaga tcaacgagaa caagctggtt atgaagcctg caggtgagtg
     6481 agccgaccgg cgcgcctgtg cggcctcaca tcctcgtcgg catcacgggc ggcatcgccg
     6541 cctacaaaag cgtgcaactc gtgcgcaccc tctgcctcgc aggctgcgac gtgcgggtgg
     6601 tcatgaccga ggcggcgacg cgtttcgtcg gccgggcgac cttcgaggcc atcagcggca
     6661 acccggtgta cgagggcctc tacgaaggcg tcgccgaggt gcggcacatc gccctcgcgc
     6721 tctggtgcga cgagatcgtc atcgcgccgg ccacggccga cacgctcgcc aggctcgccg
     6781 caggccgtgc cgacgacctc ttgacggcga ccgccttggc gggtacctcg ccggtcgtcg
     6841 tggcgcccgc catgcacacc ggaatgtggg agaacccggc gacacaggcg aacctcgcga
     6901 cgctgcgccg gcgcggcgtg cacgtcgtcg gcccggagga aggcaggctc acgagcggcg
     6961 acgtcggcgt cgggcgcatg tctgaccccg aggcgatcgc cgcggcggca cgggcgcttc
     7021 gtgtcggagg ccgggccggc cgctcagggc cccttgcagg caagagggtc gtcatcacgg
     7081 cgggcggcac gagggaaccg ctcgacccgg tgcgctttct cggcaaccgg tcgaccgggc
     7141 ggcagggtgt gcttctcgcc gaagaggccg cacggcgtgg ggccgacgtc acgctgatcg
     7201 cagcacacct ggaggtggcc gttccggccg gcgtccgacg ggtggacgtc tcgaccgcgc
     7261 tcgacatgcg ccagagtctc cgcgacacgt tggaacgggc ggacgtggtc gtcatgtgcg
     7321 ccgccgtcgc cgacttccgg ccggcggcga gcgccgtcac gaagatcaag aaagactcct
     7381 tcgacgagaa ggcggggctg cgcctcgtgc gcaaccccga catcctcgcc gaggtcggag
     7441 ctctggcaga gcgctcgttc gttctggtcg gcttcgcggc cgagacggaa ccggatcgta
     7501 ccgggctcgt cgcccgtggc cgcgccaagc ttgccgcgaa acgatgcgac ttcctcgtcg
     7561 tgaacgcggt gggctggagg agcgggttcg gcgccggcga ggcgacggcg ttcctgctgt
     7621 cggcggccga cgatatagtg acggatatca gcgggccgaa agcccgcgtt gccgaggggg
     7681 tgctcgacgc cgtcgcctcc cggctggggg gcgcgtcggc gtagggctgc cgacacaccg
     7741 gcctgtgcgg gggcaggcac ggctggaacg gcccaccggc cgaacggaaa gggcatatga
     7801 gccaactgcg cctcttcacc tcagagtccg tcaccgaggg gcaccccgac aaactctgcg
     7861 accagatctc cgacagcatc ctcgacgcgc tcctcgcggc cgacccgcag agcagggtgg
     7921 ccgtggagac gatggtgacg acggggctcg tccacgtcgc gggcgaggtc acgacccggg
     7981 cctacgtcga gattccgcag atcgtgcgcg atcgcatcac ctcgatcggc tacgactcct
     8041 cagacgtgtg gttcgacggc cgctcctgcg gcgtctccgt ctccatcggc cagcagtcac
     8101 cagacatcgc gcgcggcgtc gacaccgcgc tggaggcgcg tggcgcggcc gacacgccgg
     8161 gcggcgacga gagccggggc gactcggatc cgctcgacgg ccagggcgcc ggagaccagg
     8221 gcaccatgtt cggctatgcg acgaaagaga cgcccagcct gatgcccatg ccgatctggc
     8281 tcgcgcacag aatggcccag cgtctggccg aagtgcgtcg tgagggcatc ctcggctacc
     8341 tccgacccga cggcaagacg caggtgacgg tgggctacga cgacgggggg gtgccccgca
     8401 ccgtcgagac cgtggtcgtc tcggcgcagc attccccccg agtcacgacc gaccggctgc
     8461 gttccgacat ccgtgaggtc gtcatccgcc cggtgctcga gggctcgggg ctcgacgtcg
     8521 cctcggtgcg cacgctcatc aacccgaccg gccggttcga gatcggcggc ccgcacgcgg
     8581 atgccggact caccggccgc aagatcatcg tcgacaccta cggtggatcg gccaggcacg
     8641 gcggcggggc gttcagcggc aaagacccgt cgaaagtcga ccgctcggcc gcctatgccc
     8701 tgcgatgggt ggcgaagaac gcggtcgcgg cgggcctcgc cgatcgtctc gaggtgcaag
     8761 tcgcgtacgc catcggctcg gcccggcccg tcggcctcta cgtcgagtcg ttcggcaccg
     8821 ggcacgtgcc cgaccagcgg atcatcgccg cgatccgtgc cgtcttcgac ctgcgcccgg
     8881 ccgcgatcat ccgcgacctc gacctgttgc ggcccatcta cgcccagacg gcggcctacg
     8941 ggcacttcgg ccgcgagctg gccgacttca cgtgggagcg caccgatcgc gtcgagcggc
     9001 tgcgggacgc cgctttttag ggccatgccc ccgtacgcct tcgtcgcgcg catcgtgctg
     9061 gagagcctgc tgccgcagct cgaccatccg ttcgaatacg gtgtgcccgc gcgcctcgcc
     9121 cagcaggtga ctccgggtgt gcgggtgcgg gtgccgcttc gccgcggcag ccagcacgtg
     9181 acgggattcg tggtggagac cgcccgggag cccgcgcatg acggtgagct gagcgaggtg
     9241 gaaggcgtgg tctccgtgtt gccggtactc gcaccggagg tgtggaagct cgcccgcgcc
     9301 gtcgccgacc gcggggcagg cacggccgcc gacgtgctgc ggctggcgat cccggcccgg
     9361 gccgcccggg tcgagatgtc ggggcccggc gaggagccgg gcgcgccgct cccgcagcct
     9421 ccggcggcgc ccggacaccc ggccggatac ttcgcggggc acctcgaagg gcgctcccgc
     9481 ctcgccttga cggttccgcc gggcgtcgtg cgcttcccgg ccggacggcc gggcggcgag
     9541 cgctggctgc gttcctgggc cccgactctc gcgtcggccg cggtcgcgtg cctgcgccgg
     9601 ggggagagtg ccatcctcgc cgtgcccgac caccgagaca ccgacgaggt ggaggccgcc
     9661 ttggcgggtg cgggcgcggc cgcgtggacg gcgcgcatcg acgcgaccgg aagcaacacg
     9721 cgccgctacc gggcctttct gtcgtgcctg gcggggccgc ggatcgtgat cggcaaccgg
     9781 tccgcgctct acgcgccggc cacgaagctc gggctcatcg ccgtgtggga cgacggcgat
     9841 ccgattcatg cggagccccg cgccccgtac gcgcacgcac gcgacgtggc gcttctgcgg
     9901 cagcaacagc agtcgacggc gctgatcttc gcggcctatg cccgcagcac cgccgtcgag
     9961 cgtctcgtgc ggatcggctg gctcgccgcc gccgggccgg agcgcgtcac caggccgcgc
    10021 gtcatcgtga cgagcaggca ggccgacgac ggccgcggcg cccgcatccc gtccggcgcc
    10081 ttccaggccg cccaggaagc gtcgcgggac ggtcctgtgc tcgtccaggt ggccaggccc
    10141 ggctatgccc cggtgctctc ctgcgacacc tgccgtgagc cggcccgctg cccgcactgt
    10201 cacggcccgc tcgggctggc ggcgccgggg gctgcgcctg cctgtcgccg ctgcggccgc
    10261 agcgtgccgg gctggtcctg cccggtctgc ggcggcacgc ggctgcgaat gatcggcgtc
    10321 ggagcgagcc gcaccgccga ggagctcggc cgtgcgttcc ccggcctctc ggtcgtcgtc
    10381 gccgacggtg aacatcccgt cgatcgcatc cccgcgacac ccgcgctcgt catcgcgacc
    10441 cgcggcgccg agccgagcgc cgagggcggg tatcgggcgg tccttctgct ggatgccgcg
    10501 cgcatgttgc gccgcgagtc gctgtgggtg gcggaggact gcctgcgggt ctggacgaac
    10561 gcggccgcgc tggccgcgcc cggcgcgccc gtcgtgctcg tcggcgtcgc ggggcccctc
    10621 gccgacgcgt tcagcacgtg gcgacacgaa cgctacgccg aacgggagct cgacgatcgg
    10681 gcgcttctgc gcctgccccc ggccgtgcgc atagcgaccg tcacggggcg ccctgccgcg
    10741 ctcgaggccg cccgggaggc cttg
//
LOCUS       DASTNL010000162         8721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_665811, whole genome shotgun sequence.
ACCESSION   DASTNL010000162 DASTNL010000000
VERSION     DASTNL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665811"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..609
                     /locus_tag="VFQ96_05000"
     CDS             19..609
                     /locus_tag="VFQ96_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0257181.1"
                     /translation="MIAVLRARAPHDYDHVLDVLAEGGVRFAEVTLSTPGTLDYLPTL
                     VARGDLTVGVGTITTVAEAERAIDGGAAYLVTPVTVVPIVELAVSRGVPVFPGGLTPT
                     ELYAGWQAGASAVKIFPAQTVGAAFGKHLRGPFPDLQFVPSGGVGLNDIPAWLAAGAL
                     AVSVGGPLIGDALRGGDLDALAARTHEVVAAAEGAR"
     gene            606..1691
                     /locus_tag="VFQ96_05005"
     CDS             606..1691
                     /locus_tag="VFQ96_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0257182.1"
                     /translation="MSPARRADDEPPAGYAVTIGETMALLRQLEAGPLQHATTLGLGI
                     GGAESNVAIGLQRLGVPAAWVGRLGADSLGELVIRQIRGEGVLVKAVRDPDAPTGLMV
                     KEHRSALDTRVWYYRAGLAGARLSPADIDLDLVREARLLHVTGITLALSATAAGAVFD
                     AVRVARKAGVPVSFDLNNRSALWSRTAAQEAYRAMMPLADIVFAGDDEAEIALGPTTA
                     ATRAGTPGGRQDAAPQALQGAGHAAAAGDTDPFANGLRLARGLIALGAGEAVIKLGAR
                     GAAAVVAGAEYRQQAVPVPVVDTVGAGDAFVAGYLAELLAGAPVATRLHTAATAGAYV
                     CTVAGDWEGLPRRDELATLGSGDPVSR"
     gene            1935..2771
                     /locus_tag="VFQ96_05010"
     CDS             1935..2771
                     /locus_tag="VFQ96_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU0257183.1"
                     /translation="MPESVLLEHSRLRAWIEPDRLLPGAYQLIVDGTPQSHVNTLDPT
                     DLTYEYVRRMAHVIDLMRPPGLALTAVHLGAGALTLPRYVEATRPGSRQQVIELEERL
                     VELVRQSLPWHPASIRVRYGDARATLGRLPAGLRGTADLVVVDVFRGARTPAHVTSRE
                     FYAEVKTLLAQGGVVLVNVADGPPLRFARAQAATVQSVFTAACALAENSVLKGRRFGN
                     IVIAASDAALPLAGLPRLLASGAYPAKAVVGAEFARFAAAPVTTDSNAVPSPEPGHLF
                     GG"
     gene            2947..3549
                     /locus_tag="VFQ96_05015"
     CDS             2947..3549
                     /locus_tag="VFQ96_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0257184.1"
                     /translation="MGFILGWDPETLRENVDLRAIGERLQELGGMRSLAALSEKASLL
                     CLADQLDEALATANQALRQARFTGDRETAARARVRRASVLRRQGKTADAVKDLSDVVA
                     EAATHEWRHTEALARAHRAGAYFDAEDLEAAAGDLRTSADLQRRLQVADAELENTLAA
                     LEIVEARRDERRRLGVDKPARPTHREGPDAARHEPPRTTP"
     gene            3585..4043
                     /locus_tag="VFQ96_05020"
     CDS             3585..4043
                     /locus_tag="VFQ96_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEU0257185.1"
                     /translation="MAELDRVRLWADALIALHLDPGWTFAFDTAKTRAGLCSYARKRI
                     SVSRHLAARYGDDEIHQVLLHEVAHALAGPGAGHGPAWLGVARRIGYAGGRLHDGAVA
                     EDLAPWVGTCPRGHTHYRYRTPRRPLSCRLCGRGFDPANLITWRQRPPRP"
     gene            complement(4012..4845)
                     /locus_tag="VFQ96_05025"
     CDS             complement(4012..4845)
                     /locus_tag="VFQ96_05025"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU0257186.1"
                     /translation="MKEPLAPEAATQSPYQVRFDWGLAGLRSIAQQAEVIAVVDALTF
                     TTTLVCAVGCGFDVVPCEDPRDKAGVAQRSGALVAGERGDPGLTLSPASFTPDNVAAA
                     REAWETRPQAGADAGGRPRLVVASRNGSPLAAAAASFGVPVVALSPGNAAAVAQWLIR
                     RQEERAARQRVAVVAAGEVRRDGTLRFAAEDLLTAGAFVAACSAVGLDHSSPEAAAAG
                     AAYEGLTRGLRHLLIASVTGQQLVAGGEKADIALALAESPVVPVLHDGIFTAAGAFVA
                     R"
     gene            4899..>8721
                     /locus_tag="VFQ96_05030"
     CDS             4899..>8721
                     /locus_tag="VFQ96_05030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEU0257187.1"
                     /translation="MSEARFSPATRAWFDGSFRAATPVQAEAWEAIAGGGHVLVVAPT
                     GSGKTLAAFLWAIDRLLHRPVSGERPEAGTRVLYVSPLKALAVDVERNLRAPLAGVAH
                     AAARLGERVAGVTVGIRSGDTSQAERRRLRRAPPDILITTPESLFLMLTSQAREGLRQ
                     VQTVIVDEIHAVAATKRGSHLALSLERLDAMLERPAQRIGLSATVRPAEEVARFLGGA
                     APVHIVNPPATRSLDVRVVVPVEDMTQPPASAADTGERAGSIWPHVEDAIVGRILQHR
                     STIVFVNSRRLAERLTANLNEIYATRQKDGTNEGDAGAPEPVPVLARAHHGSVAHAER
                     ERIEDDLKRGALRCVVATSSLELGIDMGEVDLVIHVEAPPSVAAGVQRIGRAGHQVGG
                     VSHGLIFPKHRADLVHAAVAAERMTAGLIESIHVPQNPVDILAQQTVAAAAMDELEVA
                     AWFETVRRSAPFHTLARSVFDATLDMLAGRYPSDGFAGLRPRIVWDRDAGRITGRPGA
                     ARLAVTNGGTIPDRGAFGVFVAGSVGSADGSARASARVGELDEEMVYESRVGDVFALG
                     ATSWRIQEITHDRVVVVPAFGEPGRTPFWHGDAPARPAESGRAIGAFERELVAHPDPE
                     RLRRAGLGHNAAANLTAYLAEQAQAAGLPTDRTLVVEQVRDELGDWRVILHSPYGMQV
                     HAPWALAVGRRVRESYGLDGNCVAADDGIIVRVPDGDADPPGAELFVFDADEVPQIVT
                     EEVGSSALFAARFRECAARALLLPRYRPGRRAPLWQQRQQASQLLEVARAYPTFPIIL
                     ETVRECLHDVYDIDALAGLMRQIAARQVRLRQATTAEPSPFARSLLVGYVSAFMYEGD
                     NPLAERRAAALSVDSALLADLLGHVELRELLDADVIADLELSLQRLSPGRRLGADVEG
                     VADLIRILGPLGTDELRARLEGDPPTATVAGLAADLVRSHRAIDVQIAGQPMLAAIED
                     AARLRDALGLATPAGIPRAFTEPVADPLGDLVRRHARTHGPFTAGELAGRLGVGPAAV
                     LPVLQRLKADGALAVGEFRPDAQGTEWCDANVLRRIRRLCLAALRHEVEPVDQGAFAR
                     FVPAWQHTGPHRLRGLDGVLAIVEQLEGVPLPASAWESLVLPARVADYAPAMLDELTS
                     GGEVLWSGSGQIGGQDGWVSLHTAQGAATSLPAPAGLADEAGTGTARTGDGPTAEGLR
                     PDTESLERRILAALGSGGAFFFRQLSQAVGSLDDAALAAALWRLVWDGRATNDTFAPL
                     RALVGARGAHRR"
BASE COUNT         1147 a         3252 c         3191 g         1131 t
ORIGIN      
        1 ggactctacg aacaccccgt gatcgcggtg ctgcgcgccc gggcgccgca cgactacgac
       61 cacgtgctcg acgtcttggc cgaggggggt gtgcgtttcg cggaggtcac gctcagcacg
      121 cccggcaccc tcgactacct gcccacgctc gtcgcccgcg gcgatctcac ggtcggcgtc
      181 ggcacgatca cgaccgtggc cgaggccgaa cgagccatcg acggcggcgc cgcctacctc
      241 gtgactcccg tgaccgtcgt gccgatcgtc gagctcgccg tctcgcgcgg cgttcccgtc
      301 tttcccggcg gcctgacgcc gacggagctg tacgccggct ggcaggccgg cgcgagcgcc
      361 gtgaagatct ttccggccca gacggtgggc gcggccttcg gcaagcacct gcgcggcccg
      421 ttccccgacc tgcaattcgt gccctccgga ggggtgggcc tcaacgatat ccccgcctgg
      481 ttggcggcgg gcgcgctcgc cgtgagcgtc ggcgggccgc tcatcggcga cgcgttgcgg
      541 ggcggcgacc tcgacgcgct cgccgcccgc acccacgagg tcgtcgccgc ggccgagggc
      601 gcccggtgag cccggcccgg cgcgcggatg acgagccgcc tgccggctac gccgtcacca
      661 tcggcgagac catggcgctc ctgcgccagc tggaggccgg cccgctgcag cacgcgacga
      721 cgctcggcct cggcatcggc ggggccgaga gcaacgtggc gatcggtctg caacgcctcg
      781 gcgtgccggc cgcctgggtc ggccggctcg gggcggactc gctcggcgag ctggtgatcc
      841 ggcagatccg gggcgaaggc gtcctcgtga aggccgtgcg cgaccccgac gccccgacgg
      901 gtctcatggt caaggagcac cgctcggcgc tcgacacccg ggtctggtac taccgggcgg
      961 gactcgccgg cgctcggctg agccccgccg acatcgacct cgacctggtg cgcgaggcga
     1021 ggctgctgca cgtcacgggt atcacgctcg cgctctccgc cacagccgcg ggcgccgtct
     1081 tcgacgccgt gcgtgtggca cgcaaggccg gggtgccggt gtcgttcgac ctcaacaacc
     1141 gcagcgccct gtggagccga acggcggcac aggaggcgta tcgcgcgatg atgccgctgg
     1201 ccgacatcgt cttcgcgggc gacgacgagg ccgagatcgc tctcggcccc accacggcgg
     1261 ccacgcgcgc gggcaccccc gggggccggc aggacgcggc gccccaagcc ttgcaagggg
     1321 cggggcacgc cgcggcggcg ggcgacacgg acccgttcgc gaacggtctg cgcctggccc
     1381 gcgggttgat cgcgctcggc gcgggggagg ccgtcatcaa gctgggcgcc cgcggtgccg
     1441 cggccgtcgt ggcgggcgct gagtaccgtc agcaggcggt gccggtgccc gtcgtcgaca
     1501 ccgtcggcgc cggggacgcg ttcgtcgccg gctacctggc ggaacttctc gccggcgcac
     1561 ccgtggcgac ccgcctgcat acggccgcca cggccggcgc ctacgtctgc acggtcgccg
     1621 gcgactggga gggcctgccc aggcgtgacg agctggcgac gctgggctcc ggcgaccccg
     1681 tgagccgatg agcgtgccgg tcggcctgcg ctggccgatg agcacgggag ccgaggggcg
     1741 ccccggccgg ccgcagggcg gcccggtgag cctgcgagcc gacgcacgtg cgaagaggcg
     1801 gcccgggggc cgccgactct cgaacagacg gcccgcggac ccggcccgtc atgcacggcg
     1861 cggctgacgc gcgcggccgg caccgccggc tgcggccgac cgcaggccac ggcgcggccc
     1921 ggatcgcggc gaccctgccg gaatcggtgc ttctggagca cagcaggctg cgcgcgtgga
     1981 tcgaacccga ccgtctgctg ccgggcgcct atcagctgat cgtcgacggc accccgcagt
     2041 ctcacgtcaa caccctcgac ccgacggatc tcacctacga gtacgtgcgc cgcatggccc
     2101 acgtcatcga cctcatgcgg ccgccgggcc tggccctgac ggcggtgcac ctgggcgccg
     2161 gcgcgctcac cttgccgcgc tacgtcgagg cgacccgccc cggttcccgg cagcaggtca
     2221 tcgagctgga agagagactc gtggagctcg tgcggcagag cctgccgtgg catcccgcct
     2281 cgattcgcgt tcgctacgga gacgcccgtg cgacgctcgg gcggctgcct gccgggctgc
     2341 gtggcacggc ggatctcgtc gtcgtcgacg tgttccgcgg cgcgcgcacg ccggcgcacg
     2401 tcacaagccg cgagttctat gcggaggtga agactctcct tgcacagggc ggtgtggttc
     2461 tcgtaaacgt cgccgacggg ccgccgctgc gcttcgcccg cgcgcaggcc gccaccgtgc
     2521 agagcgtctt caccgccgcc tgtgcactgg ccgagaactc ggtgctgaaa ggacgccggt
     2581 tcggcaacat cgtcatcgcc gcgtctgacg cagcactgcc gctggccggg ctgccccgcc
     2641 tgctggcctc cggcgcctac cccgccaagg ccgtcgtcgg tgccgaattc gcgcggttcg
     2701 cggccgcccc cgtgacgacc gattccaacg ccgtgccctc ccccgaaccc ggccacctgt
     2761 tcggcggctg aaccgcgccc gcccggcttg ggccaagccc gcggcgaggc cggagccggg
     2821 cgcgccaccg aagaaggtcc ctccgggcgc gttaggttaa agggcgcgcc ccgctccggc
     2881 cgaacccggg gcatcgcccc acgcgggccg agccggccgg cgcgacgtga ccgagggaag
     2941 gggagcatgg gcttcatcct gggctgggat cccgagaccc tgcgagagaa cgtcgacctc
     3001 cgcgcgatcg gtgagcgcct gcaggagctg ggcggtatgc gcagcctcgc ggcgctcagc
     3061 gagaaggcct cgctcctgtg cctcgcggac cagctcgacg aggcgctcgc caccgccaac
     3121 caggcgctgc ggcaggccag attcacgggc gaccgagaga ccgcggcgcg cgcccgggtg
     3181 cgccgggcaa gcgtgctccg ccggcagggc aagacggccg acgccgtcaa agacctgagc
     3241 gacgtcgtcg ccgaagccgc gacccacgaa tggcgtcaca cggaggcgct cgcccgagcg
     3301 catcgggccg gcgcctactt cgacgcggaa gacctcgagg cggccgccgg cgacctgcgt
     3361 acgagcgccg acctgcagcg caggctccag gtagccgacg ccgagctgga gaacacgttg
     3421 gcggcgctcg aaatcgtcga ggcacggcgt gacgagcggc ggcgcctggg cgtcgacaag
     3481 cccgcccggc cgacgcaccg cgaaggcccc gacgcggccc gccacgagcc gccccgcacg
     3541 accccctgac ctcccactcg tcggccgccg ctacgatcgg gggtgtggcg gagctcgatc
     3601 gggtgcggct gtgggctgac gccttgatcg cgttgcacct cgacccgggc tggacgtttg
     3661 cgttcgacac cgcgaagacg agggccgggc tctgcagcta tgcgcgcaag cggatcagcg
     3721 tgtcacgcca cctggcggcg cgctacgggg acgacgagat ccaccaggtt ctgctgcacg
     3781 aggtggcgca cgcgctggcg ggtcccggcg ccggccacgg gcccgcctgg ctcggggtgg
     3841 cgaggagaat cggttacgcc ggcgggcgcc tgcatgacgg cgccgtggcg gaagacctcg
     3901 caccgtgggt cggaacatgc ccgcgcgggc atacccacta tcgctaccgg acgcccaggc
     3961 ggcccctgtc gtgccggctg tgcggccgag ggttcgaccc ggcgaacctc atcacctggc
     4021 gacaaaggcc cccgcggccg tgaagatgcc gtcgtgcaag acgggtacga ccgggctttc
     4081 ggccagggcg agcgcgatgt cggccttctc gccgcccgcg accagctgtt ggccggtgac
     4141 ggaggcgatg agaaggtggc gcagcccgcg cgtgagcccc tcgtatgcgg cgccggccgc
     4201 ggccgcctcc ggcgacgagt ggtcgagacc gacggcgctg caggccgcga cgaacgcgcc
     4261 ggcggtaagc aggtcttcgg ccgcgaaccg caaggtcccg tcgcgacgca cctcccccgc
     4321 cgcgacgacg gcgacgcgct gcctggccgc gcgctcctct tgacgccgga tgagccattg
     4381 ggcgacggcc gcggcgttgc ccggcgagag ggcgacgacg ggcacgccga acgaggccgc
     4441 cgcggcggcc agcggcgagc cgttgcggga cgcaaccacg aggcgagggc gcccgcccgc
     4501 gtcggcaccg gcctgcggcc gggtctccca cgcctcccga gcggcagcca cgttgtcggg
     4561 ggtgaagctc gcgggcgaga gggtgagccc cggatcgcct cgctcaccgg cgacgagcgc
     4621 gccggaacgc tgggcaaccc cggccttgtc gcgggggtct tcgcagggca cgacgtcgaa
     4681 gccgcatccg acggcgcaga cgagcgtcgt cgtgaaggtg agggcatcga ccaccgcgat
     4741 cacctccgcc tgctgcgcga tggagcgcag acccgcaagg ccccagtcga aacggacctg
     4801 gtaggggctc tgggtggcgg cctcgggggc caacggctct ttcaccccgt catggtaccg
     4861 tccgcggaag cgtcgccggt ctgtgctgag atgacggtat gagcgaggca cgcttctccc
     4921 cggcgacccg cgcctggttc gacggatcgt tccgggcggc gacgccggtg caggcggagg
     4981 catgggaggc gatcgcgggc ggcgggcacg tgctcgtcgt cgcgccgacc ggttcgggca
     5041 agaccctcgc cgcctttctg tgggcgatcg accgcctgct gcaccggccc gtctcgggcg
     5101 agagacccga ggcaggcacg cgggtgctct acgtctcacc actgaaggcg ctcgccgtcg
     5161 acgtcgaacg caacctgcga gctccgcttg cgggcgtggc gcacgccgcg gcgaggctcg
     5221 gtgagagggt cgcgggcgtc acggtcggca tccgctcggg cgatacgtcc caagccgagc
     5281 gccgccggct gcggcgagcg ccgcccgaca tcctcatcac cacgcccgag tcgctgttcc
     5341 tcatgctcac ctcccaggca cgcgaaggac tgcgccaggt gcagacggtc atcgtcgacg
     5401 agatccacgc ggtcgccgcc acgaaacgtg gttcccacct ggcgctcagc ctggaacggc
     5461 tggacgcgat gctggagcgc ccggcgcaac gcatcggcct ctcggcgacg gtgcggcccg
     5521 ccgaagaggt ggcgcgcttt ctcggcggcg ccgcccccgt gcacatcgtc aatccgccgg
     5581 cgacgaggag cctcgacgtg cgcgtcgtcg tgccggtcga ggacatgacc caaccgccgg
     5641 cctccgccgc cgacacgggc gagcgggccg gctcgatctg gccgcacgtc gaagacgcga
     5701 tcgtcgggcg catccttcag catcggtcga cgatcgtctt cgtcaattcc cgcaggctgg
     5761 cggagcgcct caccgccaac ctcaacgaga tctacgccac ccgccagaaa gatggcacga
     5821 acgagggcga cgccggcgcg ccggagcccg tgccggtctt ggcacgcgcc catcatggct
     5881 ccgtcgcgca cgcggaacgg gagcgcatcg aagacgactt gaagcgcggc gcgctgcgct
     5941 gcgtcgtcgc gacctccagc ctggagctcg gcatcgacat gggcgaggtc gacctcgtca
     6001 tccacgtcga ggcgccgccg tcggtcgcgg ccggcgtgca gcgtatcggg cgggcggggc
     6061 atcaggtcgg cggcgtctct cacggcctca tctttccgaa acaccgcgcc gatctcgtcc
     6121 acgcggccgt cgccgcagag cgcatgacgg ccggcctcat cgagagcatc cacgttcccc
     6181 agaacccggt cgacatcctc gcccagcaga cggtcgcggc ggccgcgatg gacgagctgg
     6241 aggtggctgc ctggttcgag acggtgcggc gcagcgcgcc gttccacacc ctcgcccgca
     6301 gcgtcttcga cgcgacgctc gacatgctcg ccggccggta cccttccgac gggttcgccg
     6361 gccttcgccc tcgtatcgtg tgggacaggg acgcgggccg catcaccggc cggcccggcg
     6421 ccgcccgcct cgcggtgacg aacggcggca cgattcccga ccgcggcgcg ttcggcgtgt
     6481 tcgtcgccgg atccgtgggt tccgcggacg ggtcggcgcg cgcgagcgcc cgggtgggtg
     6541 agctcgacga agagatggtc tacgagtcgc gggtcggcga cgtcttcgcg ctcggcgcga
     6601 cgagctggcg catccaggag atcacacacg accgggtcgt cgtcgtcccg gccttcggcg
     6661 agccgggccg cactccgttc tggcacggcg acgcgccggc gagacctgcc gagtcgggcc
     6721 gcgcgatcgg cgcgttcgag cgcgagctcg tggcccaccc cgacccggaa cgcctgcgcc
     6781 gggcagggct cggccacaat gcggccgcga acctcaccgc ctacctcgcc gagcaggccc
     6841 aggccgccgg cctgcccacc gaccggacgc tcgtcgtcga gcaggtgcgc gacgagctgg
     6901 gcgactggcg cgtcatcttg cattccccgt acggcatgca ggtgcatgcg ccgtgggcgc
     6961 tcgcggtcgg gcgacgggtg cgggagagtt acgggctcga cggcaactgc gtcgcggccg
     7021 acgacggcat catcgttcgg gtgccggacg gcgacgccga cccgccgggc gcggagctct
     7081 tcgtcttcga tgccgacgag gtgccgcaga tcgtgacaga ggaggtcggc tcctcggccc
     7141 tcttcgccgc gcgtttccgg gaatgcgccg cccgcgcgct gctcttgccc cgctatcgcc
     7201 ccggccggcg ggcgccgctg tggcagcagc ggcagcaggc ctcccagctc ttggaggtcg
     7261 cgcgggccta cccgacgttc ccgatcatcc tcgagacggt gcgggaatgc ctgcacgacg
     7321 tctacgacat cgacgccctc gcgggcctca tgcggcagat cgcggcgcgg caggtgcggc
     7381 ttcggcaggc caccacggcc gaaccctcgc ccttcgcccg cagtctgctc gtcggctacg
     7441 tctccgcctt catgtacgaa ggcgacaatc ccctggccga gcgccgcgcg gccgctctca
     7501 gtgtcgactc cgccctgctg gcagacctgc tggggcacgt cgagctgcgg gagctgctgg
     7561 atgccgacgt catcgccgac ctggagctct cgttgcagcg actctccccc ggccggcgcc
     7621 tcggcgccga cgtggagggt gtggccgacc tgatccgtat cctcggcccg cttggcaccg
     7681 acgagctgcg ggcgaggctc gagggcgacc cgccgacggc gacggtcgcc ggcctcgccg
     7741 ccgacctggt gcgctcccac cgcgcgatcg acgtgcagat cgcggggcag ccgatgcttg
     7801 ccgccatcga agacgcggcg cggctgagag acgcgctggg gcttgcgacg ccggccggca
     7861 taccgcgagc cttcaccgag ccggtcgccg acccgctggg cgacctcgtg cgccggcacg
     7921 cccgcacgca cggaccgttc acggccgggg agctcgccgg ccggctgggc gtcggcccgg
     7981 ccgccgtgct gccggttctg cagcgcctca aggccgatgg cgcgctcgcg gtcggcgagt
     8041 tccggcccga cgcgcaaggc acggaatggt gcgacgcgaa cgtgctgcgc cgcatccggc
     8101 ggctctgcct cgcagccctc cggcacgagg tggagcccgt ggatcagggg gccttcgccc
     8161 gcttcgtgcc ggcctggcag cacacgggcc cgcaccggct gcgcgggctc gacggcgtct
     8221 tggccatcgt cgaacagctg gagggcgtac cgctgcccgc ttccgcgtgg gagtcgctcg
     8281 tgctgccggc cagggtcgcg gactacgcgc cggcgatgct cgacgagctg acctcgggcg
     8341 gcgaggtcct ctggtcgggt tcgggccaga tcggcggcca agacggttgg gtgagcttgc
     8401 acaccgccca gggcgccgcg acgagcctgc cggcgcccgc aggccttgcc gacgaggcgg
     8461 gcacaggcac cgccagaacc ggtgacggcc cgacggccga agggctccgg cccgacacgg
     8521 aaagcctgga gcggcgaatt ctcgccgcgc tgggctccgg cggggcgttc ttctttcgcc
     8581 agctctcgca ggccgtcggc agcctggacg acgcggctct cgccgccgca ctctggcgcc
     8641 tggtgtggga cggccgggcg acgaacgaca cgttcgcacc gttgcgggcg ctcgtcggcg
     8701 cccgaggcgc ccaccggcgg c
//
LOCUS       DASTNL010000163         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_852435, whole genome shotgun sequence.
ACCESSION   DASTNL010000163 DASTNL010000000
VERSION     DASTNL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852435"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFQ96_05035"
     CDS             complement(<1..1292)
                     /locus_tag="VFQ96_05035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0257188.1"
                     /translation="MTRSEATYPRGGARRGEARHVVVAGMGALTPCGSGVGALWGAVR
                     NGRSAIEVLDDNVFTGLPVRIGGVVRGFDAEAVLSRALARRLMPAQQWAVAAADEAMR
                     QVGVALPSPGSDGPAGWARLPWSPHRFSIITATGSGPVDATLQAEAAFQARGPRGVPV
                     TFAAYGAPDAVGTVLAKRYGATGASHALCATCASGSSALGQGMRLIRHGYADAVLVVA
                     VEDCLNPVNLSSNANLRALATGYEDNPAEACRPFDRGRRGFVMAQGAAAMLLTAAPSD
                     GWRNSDTDGRGAGGDRAVIPALAELAGFGAAEDAYHPTSPHPEGLGAALAIEDCLADA
                     GVTPDEVAHINAHATGTPAGDAAEIAAFDRVFHEAGRRIPISATKAVTGHLMGASGVA
                     EAIIAVETLRSGVLPGTGNLADSEFPDWDIVAGPSRPAP"
     gene            complement(1289..2788)
                     /locus_tag="VFQ96_05040"
     CDS             complement(1289..2788)
                     /locus_tag="VFQ96_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEU0257189.1"
                     /translation="MPITQSILDVAERHPGRVAIADRRERLDYAGLVADSRRTVAAVR
                     TLGEGSRVRTIGEACGIPVTAFCLFSAFHTARLVAGLAGYRAVSAVIDPRWPIDHQAG
                     AILSCGVSLVVADQTGLADALAARGWGGRIVSLPEFQRVAASAAPAEPPTARAGSEPF
                     LLLFSSGTTHAPKAFVKTRYQYRQNFTVSSAHLEPLPGEATLAPGPVSYSLTLYAVIE
                     ALASGGSIHLADTADPRAAARRIPEEGVTRVVAVPALVRGLLAAARDGELAGLRLVVS
                     GGANLTEETRRLFHDLLPNAQLISYYGAAEIGFIGDSRSGDGTLIDVYPGIGVRVASD
                     AGEPLPAGEAGTVWVRAEACSDDYVAGTASEPLQVDGWASVHDQGSMAGGRLRLAGRA
                     GDIITTGGHNVSLNEVERAFDGMPGLGVACAVAMPSAQLGHVVALVVEGEAPPKAELL
                     SRARARLAPQFVPHRWFQVPRLQRTVGGKIRRGAVADQVAADGPEVVRL"
     gene            complement(2938..3294)
                     /locus_tag="VFQ96_05045"
     CDS             complement(2938..3294)
                     /locus_tag="VFQ96_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257190.1"
                     /translation="MTDAPLPPEPGQGEWEGRAQPPHISGFRIDRYHLAEAGTLDHRG
                     RRYRLRLTDDDTWSIRGPSGEVIGTLIILIPQTGGGVPVYATRRPGSTRLEHQGTDWR
                     SITAALVNETLEPPAR"
     gene            complement(3315..3995)
                     /locus_tag="VFQ96_05050"
     CDS             complement(3315..3995)
                     /locus_tag="VFQ96_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0257191.1"
                     /translation="MTPPAEGTADGLEPNGVGGKADAAAGLTGVAPTDAAVASEAASA
                     GKAPLRVVIVNGSPSDPATSKTVALADLAADEVRERWDATVARVDVYAIGPDLTGAIL
                     RDDAGPQAGRALALVEGADLLLVAVPVFRGSYPGMFKHFLDLIEPYALAGKPTLLLAT
                     GGSERHLLVIDHELRPLFAFFQAFVAPVGVYVGAESFDGTLILDPAVQTRLRLALGDL
                     VPLLGPTR"
     gene            complement(3992..5101)
                     /locus_tag="VFQ96_05055"
     CDS             complement(3992..5101)
                     /locus_tag="VFQ96_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0257192.1"
                     /translation="MFEIGVITFGEVTTDPVTGVLPTARQRVKETIEQAKVADEVGLD
                     VFGIGEHHRSDFVASTPAILLAGAATATKNIRLTSSVSVLSTSDPVRVYEEFATLDLL
                     SGGRAEVIAGRGSYTESFPLFGYSLEEYDELYEEKVGLLLKIRDENPVTWSGRMRAPL
                     EAADVAPRALQEKMPIWRAVGGSPASAVRAGRARMPLMLAFIVGPLAAFQRLAGYYLQ
                     AADEAGMPAAETRIGAAVHGFVGATSQDARDTMFPYFSRGMNENNHQRGVGFDLPRAA
                     FEAQATPGGMPVVGSPQEVIDKIMTYHEVYGIDRILLQMGFGGVPQRDHLRAIELLGT
                     EVAPVVRRELGGADTGGDGEAADASSSADATRINA"
     gene            5241..6230
                     /gene="hemB"
                     /locus_tag="VFQ96_05060"
     CDS             5241..6230
                     /gene="hemB"
                     /locus_tag="VFQ96_05060"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0257193.1"
                     /translation="MSDVLHPTVRPRRLRTTAALRRLVAETRIDESRLILPVFVREGA
                     TEAVPIEAMPGVVQHSLDSLKKAAADAAQAGLGGIMLFGVPEHRDAVGSGATDPRGIL
                     NVATRAVAQEVGDALVVQTDLCLDEFTDHGHCGVLDGQGRVDNDATLLRYRDMAVAQA
                     EAGSQVVGLSGMMDGQVAAVRDALDQSGHIDTVILGYAAKYASSFYGPFREAVDSQLQ
                     GDRRTYQQDPANRREGLREAELDLAEGADVVMVKPAMAYLDVLSDVARASRVPVWAYQ
                     VSGEYSMIQAAAARGWIDRERIVTETLTGILRAGADAVLTYFALEVARRSARR"
     gene            6227..>6380
                     /locus_tag="VFQ96_05065"
     CDS             6227..>6380
                     /locus_tag="VFQ96_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU0257194.1"
                     /translation="MTSNDALFARARVVIPGGVDSPVRADRSVGGPPPFMVSGHGPYI
                     VDAEGRE"
BASE COUNT          891 a         2245 c         2321 g          923 t
ORIGIN      
        1 ggggcgggtc gggagggccc cgccacgatg tcccagtcgg ggaactccga atcggccagg
       61 ttgccggtgc cgggcaggac gcctgagcgc agcgtctcga ccgcgatgat cgcctctgcc
      121 acgcccgagg cgcccatcag gtgccccgtg accgccttcg tcgcgctgat cgggatgcgc
      181 cgccccgcct cgtggaacac gcggtcgaag gcggcgatct ccgccgcgtc accggctggg
      241 gtgccggtcg cgtgggcatt gatgtgggcc acctcgtcgg gggtcacgcc agcatccgcc
      301 aggcagtcct cgatcgccaa ggctgcaccc aggccctccg gatgcggcga ggtggggtgg
      361 taggcgtctt cggcggcgcc gaacccggcg agctccgcga gggccggtat cacggctcgg
      421 tcgccgccgg ccccacgccc gtcggtgtcg gagttccgcc agccgtctga gggcgccgct
      481 gtcaggagca tcgcggccgc gccctgcgcc atgacgaagc cgcgcctgcc ccggtcgaac
      541 ggccggcaag cctcggcagg attgtcttcg tacccggtcg cgagcgcccg caggttcgcg
      601 ttcgacgaga ggttgaccgg gttaaggcag tcttcgacgg cgacgaccag caccgcgtcg
      661 gcgtagccgt gccggatgag tcgcatcccc tgcccgaggg cgctggatcc gctggcgcag
      721 gtcgcgcaca gggcgtgcga ggcccccgtc gcgccgtacc gtttcgccag caccgtgccc
      781 accgcatcgg gggcgccgta ggcggcgaag gtgaccggca caccgcgcgg cccgcgcgcc
      841 tggaacgcgg cctcggcttg gagcgtcgcg tcgaccgggc cggagcccgt cgccgtgatg
      901 atggagaacc ggtgcgggct ccacggcagg cgcgcccacc ccgctggccc gtctgacccc
      961 ggcgacggga gcgcgacgcc gacctgtcgc atcgcctcgt cggcagccgc gaccgcccac
     1021 tgctgggccg gcatcagccg gcgtgcgagg gcgcgtgaca gcaccgcctc cgcgtcgaac
     1081 ccgcgcacga ccccgccgat gcgcaccggc agccccgtga acacgttgtc gtcgagaacc
     1141 tcgatggcgc tgcggccgtt ccgcacggcc ccccacaggg cgccgacacc cgagccgcag
     1201 ggggtgaggg cgcccatccc cgcgacgacg acgtgacgcg cctcaccccg acgggcgccc
     1261 ccgcgcggat aagtcgcctc gcttcttgtc acagtcgcac cacctccggg ccgtcggccg
     1321 cgacctggtc ggccaccgcg ccgcggcgga tcttgccgcc caccgtgcgt tgcaggcgcg
     1381 gcacctgaaa ccaccggtgc ggaacgaact gcggcgcgag gcgggctcgc gcacgcgata
     1441 agagttcggc ttttggcggt gcctcgcctt ctaccacgag ggcgacgacg tgcccgagct
     1501 gcgcgctcgg catcgcgacg gcgcatgcga cgccgaggcc cggcatgccg tcgaatgccc
     1561 gctcgacctc gttcagggag acgttgtggc cgccggtcgt gatgatgtcg cctgcgcggc
     1621 cggccaggcg caggcgaccg cccgccatgc tgccctggtc gtgcacgctc gcccagccgt
     1681 ccacctgcag aggctcgctc gccgtgcccg ccacgtagtc gtcggagcac gcctccgcgc
     1741 gcacccagac cgtgccggcc tcgcccgccg gcagcggctc gccggcatcg gaggcgaccc
     1801 gcaccccgat gcccggatag acgtcgatga gggtgccgtc gcccgaccgg ctgtcgccga
     1861 tgaagccgat ctcggcggcg ccgtaatagc tgatgagctg cgcgttcggc agcaggtcgt
     1921 gaaagagccg tcgggtctct tcggtgaggt tcgcgccgcc ggagacgacg agtctgaggc
     1981 cggccagttc gccgtctcgg gctgccgcga gaagcccgcg cacgagcgcc ggcacggcga
     2041 cgacccgggt cacgccctct tcagggatgc gccgtgccgc cgcccgcggg tctgccgtgt
     2101 ctgccaggtg gatgctgccg ccggaggcga gggcctcgat gaccgcatac agcgtgaggc
     2161 tgtacgagac ggggccgggc gccagcgttg cttcaccggg cagtggctcc aggtgcgcgc
     2221 tggagaccgt gaagttctgc cggtactggt agcgggtctt gacgaacgcc ttcggggcgt
     2281 gggtcgtgcc cgaggagaag agcagcaaga acggctcgct gccggcgcgg gccgtcggcg
     2341 gctcggcagg tgccgcactc gcggcgaccc gctggaattc tggcaggctg acgatgcggc
     2401 cgccccagcc gcgggccgcg agcgcgtcgg ccagccccgt ctgatcggcc acgacgaggc
     2461 tcacgccgca agagaggatg gcgccggcct ggtggtcgat cggccagcgt gggtcgatga
     2521 cggcgctgac ggcccggtag ccggccagcc ccgccacgag cctcgcggtg tggaacgcgg
     2581 agaacaggca gaacgccgtc accgggatgc cgcacgcctc gccgatcgtg cgcacccggg
     2641 atccctcgcc gagggtccgc accgcggcga cggtgcgccg gctgtcggcg accagcccgg
     2701 cgtagtcgag gcgttcccgc ctgtcggcga tcgccactcg gccggggtgg cgctctgcaa
     2761 catcgagaat gctctgtgtg atcggcatgg gcgggcaccc cctttcaccg cgcaggcagg
     2821 gtcgcggcac cagtgtagtt gtggcagatg taccgagcct agaaagtggc ggcgtactcg
     2881 acggcggtgt cggccgcctg gtgggcgtcg acgagggagt acgggctgtg gaggggctca
     2941 gcgtgccggt ggctcgaggg tctcgttgac gagggcggcc gtgatgctgc gccagtcggt
     3001 gccttgatgc tccaggcggg tcgacccggg gcggcgtgtg gcatagacgg gcacgccgcc
     3061 gccggtctgg gggatcagga tgatgagggt accgatgacc tcgccggagg ggccgcgaat
     3121 actccacgtg tcgtcatccg tcagccgcag gcgatagcgc cggccgcgat ggtcaagcgt
     3181 gcccgcctcg gccaggtggt agcggtcgat gcgaaagccg ctgatatggg gaggctgggc
     3241 gcggccctcc cactcaccct ggcccggctc gggcggcagc ggcgcatcgg tcatgagacc
     3301 agtgtgcgcc gcggctacct cgtcgggccg agaaggggca cgaggtcgcc caatgcgagc
     3361 cgcaggcggg tctgcaccgc cgggtcgagg ataagggtgc cgtcgaacga ctcggcgccc
     3421 acgtagacgc cgaccggcgc gacgaacgcc tgaaagaagg cgaaaagcgg ccgtagctcg
     3481 tgatcgatga cgagcaggtg tcgttcgctg ccgcccgtcg ccagaagcag ggtcggcttg
     3541 ccggccagcg cgtagggctc gatgaggtcg aggaaatgct tgaacattcc ggggtacgag
     3601 ccgcgaaaga ccggcaccgc gacgaggagc agatcggcgc cttctacgag agccagcgcc
     3661 cggccggcct gtggcccggc gtcgtcgcgc aggatcgctc cggtgaggtc ggggcctatc
     3721 gcgtagacat ccactctcgc gacggtcgca tcccagcgtt cgcgcacttc gtcggcggcg
     3781 aggtccgcga gggcaacggt cttcgacgtc gccgggtcgc tggggctccc gttcacgatg
     3841 acgacccgca gcggggcctt gccggcagac gccgcctcgg aggccacggc ggcatcggtg
     3901 ggcgcgacac cggtcagacc agctgccgcg tcggccttcc cgccgacccc gttcggttcg
     3961 aggccgtcgg ctgtgccctc tgcgggcggc gtcatgcgtt gatccgggtc gcgtcggctg
     4021 aggaggaggc gtctgccgcc tcgccgtcgc ccccggtgtc ggctccgccc agttctcgcc
     4081 gcacgaccgg cgccacctcg gtgccgagca attcgatcgc ccgcaggtgg tcgcgctgcg
     4141 gcacgccgcc gaaccccatc tgcagaagga tgcggtcgat gccgtacacc tcgtggtacg
     4201 tcatgatctt gtcgatgacc tcttgcgggc tgccgaccac cggcatgccg cccggggtcg
     4261 cctgcgcctc gaaggcggcc cgcggcaggt cgaaaccgac cccgcgctga tggttgttct
     4321 cgttcatgcc gcgagaaaag tacgggaaca tcgtgtcgcg ggcgtcttgg cttgtcgcgc
     4381 cgacgaaacc gtgcacggcg gcgccgatgc gggtctcggc ggcaggcata cccgcctcgt
     4441 cggcagcctg gaggtagtac ccggccaagc gttggaaggc ggcgaggggc cccacgatga
     4501 aggcgagcat gagcggcatg cgagcccgcc cggcccgcac cgccgaggct gggctgccgc
     4561 ccacggcgcg ccagatcggc attttctcct gcagcgcccg cggcgcgacg tcggcggctt
     4621 cgagcggcgc gcgcatccgg cccgaccagg tgaccgggtt ctcgtcgcga atcttcagga
     4681 gcagcccgac cttctcctcg tagagctcgt catactcttc gagcgagtac ccgaacaggg
     4741 ggaacgactc cgtgtacgaa ccgcgcccgg cgatgacttc ggcgcggccg ccggagagca
     4801 ggtcgagggt cgcgaactcc tcgtagaccc gcacggggtc gctcgtagag agcacgctga
     4861 cgctgctggt cagccggatg ttcttcgtcg ccgtcgcggc gcccgccaga aggatggccg
     4921 gggtgctcgc cacgaagtcg ctgcggtgat gctcaccgat tccgaagacg tcgaggccca
     4981 cctcgtcggc caccttcgcc tgctcgatgg tctctttgac tcgctgccgg gccgtcggca
     5041 ggacgcccgt cacggggtcg gtcgtcacct cgccaaacgt gatcacgccg atctcgaaca
     5101 tcgcttcctt tcctggacgc cctcgggccg caggccgtgt cgaccgtctt gccccggctc
     5161 tctcaagaca atccggctac ccatggcgtt attccacggc ccgaccgcgg cccgacggtg
     5221 gctagaccta cgctagagga gtgagtgacg ttctccaccc gaccgtcaga ccccgccgcc
     5281 tgcgtaccac cgccgccttg cggcgactcg tcgctgagac gcgtatcgac gagagccggc
     5341 tgattctgcc cgtcttcgtg cgcgagggcg cgacggaggc ggtgccgatc gaggcgatgc
     5401 cgggcgtcgt gcagcacagc ctcgattcgc tgaagaaggc ggcggcagat gccgctcagg
     5461 cgggcctcgg cggcatcatg cttttcggcg tgccggagca tcgggatgcg gtgggcagcg
     5521 gcgcgaccga cccgcggggc atcctcaacg tcgccacccg cgcggtcgcg caagaggtcg
     5581 gcgacgcgct cgtcgtgcag actgacctgt gcctcgatga gttcaccgac cacggacact
     5641 gtggcgttct cgacggccag gggcgggtcg acaacgacgc gacgctcctg cggtaccgtg
     5701 acatggcggt ggcgcaggcg gaggcgggct cccaggtggt cggcctctcc ggcatgatgg
     5761 acggccaggt cgcggccgtg cgtgatgcac tcgaccagtc ggggcatatc gacaccgtca
     5821 tcctcggcta cgcggccaaa tacgcctcct ccttctacgg cccgttccgc gaggccgtcg
     5881 attcgcagct acagggcgac cgccgcacct accagcaaga tccggcgaat cggcgggagg
     5941 gcttgcgcga ggccgagctc gacctcgccg agggcgccga cgtcgtgatg gtgaaacccg
     6001 cgatggcgta cctcgacgtg ttgagcgacg tcgcgcgggc gagccgggtg ccggtgtggg
     6061 cctaccaggt ctcgggcgag tactcgatga ttcaggcggc ggcggcacgc ggctggatcg
     6121 accgggagcg gatcgtgacg gagacgctca ccggcattct gcgagccggc gccgacgcgg
     6181 tgctcaccta cttcgcgttg gaagtcgccc ggcggtcggc acgccggtga cgagcaacga
     6241 cgcgctcttc gcgcgcgccc gggtcgtcat tcccggcggg gtcgactcgc cggtgcgcgc
     6301 agatcgttcg gtcggcggcc cgccgccgtt catggtctca ggccacggcc cgtacatcgt
     6361 ggatgccgag gggcgggaat
//
LOCUS       DASTNL010000164         7467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1035369, whole genome shotgun sequence.
ACCESSION   DASTNL010000164 DASTNL010000000
VERSION     DASTNL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035369"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VFQ96_05070"
     CDS             <1..1404
                     /locus_tag="VFQ96_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257195.1"
                     /translation="WTAGKEVSLTTFARESGAFYLPPSFTADTDLSPIAPAEPVIVRG
                     MGLAATDLVVLLTEGRGGRFTRDPSGLRYLPSGREPRLFLGSRRGVPYHAKITSRLKG
                     TRPGARFFTARRAAELAGRRRLDFARQVWPFIAKEMLHGYYAELFTGHPQHVRVRWAQ
                     FAERFAPLDPFGQGLRALVAASVPDPADRLDLARLTRPLAGRRFEGSGALQEALRGYI
                     RDDLRRRTLPRHSASLGLFLSLLESFGVVVGMAGADWTAASRARDLEGWWRPYFSSIA
                     SGPPARRLEELLALSRAGVVTFLGGGLEVTGDARSGTFLARGENAPGEVAAAALVDAW
                     LPAPRAAHGPDPLLRHLVDTGQAREAPLGHGRTTGRIEVVPDAFAVVGADGSPHPARY
                     ALGPFTSAPVTGAFTRPGTNGLAFRENDRLARRVLAALSKPSGPPPGRSASAAPTREH
                     RVFADTRTDRGTAYYAA"
     gene            1530..2864
                     /locus_tag="VFQ96_05075"
     CDS             1530..2864
                     /locus_tag="VFQ96_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257196.1"
                     /translation="MAQPTIDAPGTTAGAGTTTRLRRTFTIIPGVSGRQLMAGAIGNL
                     IEWFDWNAYAFLSVYFARLFFPANTPALVSLLGSFGILAVGFLCRPLAGLVLGFLSDR
                     IGRKPTLMLTVYGMGAASLIIALAPTYAQIGLAAPLLLLVSRIIQGFCIGGEYASLSA
                     FAMEMSPTGKRGRVAGYLTAIAAIGQLAVVLLIFGLSSFLSTAEMTTWGWRVVFGIGA
                     AIAFGGVWLRRGMKETLDTHRVRGEHIGIFTALRRHPRQSIMVVGIVLGFTAMVYAWG
                     AYMPAYATTSAGIAPKDTMAAMLISLLVSIAGAIPAGYLSDRYGRKATMIAAGVILSV
                     GTVPAMTFLSPWIWSLVIAQSFGLLVLDLLQASSMPAFAEMFPASFRSTGLGFPYALT
                     VGLIGGTIPMVGTQLTALHLGTFFPWYLVALMVISTLFYIGMKETAFDPLPE"
     gene            complement(2963..3517)
                     /locus_tag="VFQ96_05080"
     CDS             complement(2963..3517)
                     /locus_tag="VFQ96_05080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0257197.1"
                     /translation="MTIEQVHRWEDEPAMASESLDAEAFKKVFRRHAAGVALITADAG
                     DGPVALTATSVFSISAVPPLLAFSLSDESSAAETIGRAETLVVHLLSADQLGLAKLGA
                     TTGIDRFADDSIWERLATGEPYFPAVHTWIRGRIANRMRAGNSTVLAVRGLEARWPQA
                     PEAEAPLVYVSRTWHALDARSRIA"
     gene            complement(3723..3995)
                     /locus_tag="VFQ96_05085"
     CDS             complement(3723..3995)
                     /locus_tag="VFQ96_05085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU0257198.1"
                     /translation="MKIVAVCGAGIGTSAILKLNAERALERLDIDAVVEAGDLASVTG
                     RHRDAQVILTSPQLAGRLGPTRADVVVIDNYLDVSEIADKLERAIG"
     gene            complement(4120..4578)
                     /locus_tag="VFQ96_05090"
     CDS             complement(4120..4578)
                     /locus_tag="VFQ96_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU0257199.1"
                     /translation="MDLPPLRDPAIVLRARAEGWREAVSLAGAALERAGCTGPGYTRR
                     MIDVVEEFGAYIVIAPGLALAHARPGPDVLADGLSVVTLEGPVEFGHPHNDPVRVVVG
                     LALRTPDSHVTGVAALANVFNDPGAVERLVRAATKDDVRAVMTVGPEASS"
     gene            complement(4785..5888)
                     /locus_tag="VFQ96_05095"
     CDS             complement(4785..5888)
                     /locus_tag="VFQ96_05095"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU0257200.1"
                     /translation="MNDETLREPHLLPDGTDLGPLPKVSLHDHLDGGLRPQTVIELAD
                     QDGVEIPEHDAGRLARWFAEHADSGSLVEYLRTFDVTVSVMQTRAALTRVAGEFVDDL
                     VTDGVVYGEVRWAPEQHLSRGLTPDDAVDAVQEGIDAAVARAAAGGHEIRVGQLVSAL
                     RHRPNALEAARLAVRHRGAGVVGFDIAGPEDGFPPAGHREAFDYLAQNLFPVTVHAGE
                     AAGVESIRGALVDGRALRLGHGVRIAQDLGADGATLGPAARWVRDRRVPLELSPSSNV
                     QTGAIAPWGASLREHPFDSLYRQGFTVTVNTDNRLMSATTLTRELALLAETFGYGLSD
                     IARFQRNAAEAAFLPLPEREELARRLASAYAAV"
     gene            complement(5965..6348)
                     /gene="fdxA"
                     /locus_tag="VFQ96_05100"
     CDS             complement(5965..6348)
                     /gene="fdxA"
                     /locus_tag="VFQ96_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU0257201.1"
                     /translation="MAYVIGAACIDVMDRACVDECPVDCIYEGERSLYIHPDECIDCG
                     ACEPVCPVEAISYEDDVPDRWSDFIADNERFFAKPLPGIVEPLGSPGGAAKLGPTHVD
                     TELVAGYPPLDERDDTVDNRNGFTH"
     gene            6487..6771
                     /locus_tag="VFQ96_05105"
                     /pseudo
     CDS             6487..6771
                     /locus_tag="VFQ96_05105"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
     gene            6883..7209
                     /locus_tag="VFQ96_05110"
     CDS             6883..7209
                     /locus_tag="VFQ96_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU0257202.1"
                     /translation="MTEPDAELIEKVEEALKDVIDPELGVDIVNLGLVYGYTWDAANQ
                     RLFIKATLTSAECGHQGIIGSQIAKAIEGIVPSHVVEWVWMPAWSPARVTEDGRDMML
                     ALGFNV"
     gene            7202..>7467
                     /locus_tag="VFQ96_05115"
     CDS             7202..>7467
                     /locus_tag="VFQ96_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEU0257203.1"
                     /translation="MSDEQHPSAEAPEPRGAGPKTGRSGIAADETSAYGYLVPRIHGA
                     LTQVIDPEIRRPITELNMVPGIEVDDQADATVTIRLTTATCPNSR"
BASE COUNT         1079 a         2756 c         2506 g         1126 t
ORIGIN      
        1 tggacggccg gcaaagaggt gagcctcacg acgttcgcgc gggagtcggg cgcgttctac
       61 ctgccgccct cgttcacggc cgacaccgac ctgtcgccga tcgcgccggc cgagcccgtc
      121 atcgtgcgcg gcatgggtct cgcggcgacc gacctcgtcg tgctcctcac cgaagggcgc
      181 ggcggccggt tcacgcgcga cccctccggg cttcgctacc tgccctccgg ccgggagccg
      241 cgactcttcc tcggctcccg gcgtggcgtg ccttaccacg ccaagatcac ctcccggctc
      301 aaaggcacac ggcccggcgc ccggttcttc accgcacgac gggcggccga actggccggc
      361 cggcgcaggc tcgacttcgc ccgccaggtc tggccgttca tcgcgaaaga gatgttgcac
      421 ggctactacg cggagctttt caccgggcac ccgcagcacg tgcgcgtgcg ctgggcgcag
      481 ttcgccgagc gcttcgcccc gctcgacccg ttcggccagg gactgcgggc cctcgtcgcc
      541 gcgagcgtgc ccgacccggc agaccggctc gacctcgccc ggctcacccg gccgctggca
      601 gggcgccggt tcgagggctc cggtgcactg caagaggcgt tgcgcggcta cattcgcgac
      661 gacctgcgtc gacgcaccct gccccggcac agcgcaagcc tcgggctctt cctctcgctt
      721 ctcgagtcat tcggcgtcgt cgtcggcatg gccggcgccg actggacggc ggcgtcgcgc
      781 gcacgcgacc tggagggctg gtggcggccc tacttcagct cgatcgcgag cggcccgccg
      841 gcgcgtcgcc tcgaagagct gctcgcgctc tctcgcgcgg gcgtcgtgac gtttctcggc
      901 ggcggcctcg aggtcaccgg cgacgcccgg tcgggcacgt tcctcgcccg cggcgagaac
      961 gccccgggcg aggtggcggc cgccgccctc gtcgacgcct ggctgcccgc cccgcgcgcg
     1021 gcacacggcc ccgacccgct cttgcggcac ctcgtcgaca ccggccaggc cagagaagcg
     1081 ccccttgggc atggccgcac cacgggccgg atcgaggtcg tgccagacgc cttcgccgtc
     1141 gtcggcgccg acggctcgcc gcatccggcg cgctacgccc tcggaccttt cacaagcgcc
     1201 ccggtcaccg gcgcgttcac ccggcccggc acgaacggac tcgccttccg cgagaacgac
     1261 aggctggccc gccgtgtctt ggccgcactc tccaagccct ccggcccgcc tcccgggcgt
     1321 tccgcgtccg cggcaccgac gagggaacac cgggtcttcg ccgacacccg gacggaccgc
     1381 gggacggcat attacgccgc gtgaccccag gtttcccctt gtttctcgcc gcagcgcggc
     1441 ccgccaccga tcgtcccaag atggctctca agacatccaa gcggtcgagt ctgaacggtg
     1501 cgcggccgcc caccagagca ggagagggca tggcccaacc aacgatcgac gcacccggca
     1561 ccaccgcggg ggcaggcacc accacgcggc tacgcagaac cttcaccatc atcccgggcg
     1621 tctcgggccg gcagctgatg gcgggcgcca tcggcaacct gatcgagtgg ttcgactgga
     1681 acgcctacgc gttcttgtcg gtctacttcg cacgcctgtt cttcccggcg aacacgccgg
     1741 ccctcgtctc gctgctcggc agcttcggca tccttgccgt cggcttccta tgccggccgc
     1801 tggccggcct ggtgctgggc ttcctctccg accgcatcgg gcgcaagccg acgctgatgc
     1861 tcaccgtcta cggcatgggc gcggcgagcc tcatcatcgc gctcgccccc acctacgcgc
     1921 agatcgggct tgcggccccg cttctgctgc tcgtctcgcg catcatccag gggttctgca
     1981 tcggcggcga atacgcctcg ctgtcggcct tcgcgatgga gatgtcgccc accggcaaaa
     2041 ggggccgcgt cgccggctat ctgaccgcga tcgccgcgat cggccagctc gccgtcgtgc
     2101 ttctcatctt cgggctctcc tcgttcctct ccaccgcaga gatgacgacg tggggctggc
     2161 gcgtggtgtt cggcatcggc gcggcgatcg ccttcggcgg cgtctggctg cgccgcggca
     2221 tgaaggagac cctcgacacg caccgcgtgc gcggcgagca catcggcatc ttcaccgccc
     2281 tgcgtcgcca cccgcggcag tcgatcatgg tcgtcggcat cgtgctcggc ttcacggcca
     2341 tggtgtacgc ctggggtgcc tacatgccgg cctatgccac gacgtcggcg ggcatcgccc
     2401 cgaaagacac gatggcggcc atgctcatct cccttctcgt ctcgatcgcg ggcgcgatcc
     2461 ccgccggcta cctgtccgac cgatacgggc gcaaggccac gatgatcgcg gccggcgtga
     2521 tcctctcggt cggcaccgtg cctgcgatga cgttcctcag cccctggatc tggtctctcg
     2581 tcatcgcgca atccttcggc ctcctggttc tcgacctgct gcaggccagt tcgatgccgg
     2641 ccttcgcaga gatgttcccg gcctccttcc gctccaccgg cctgggtttc ccctacgcgc
     2701 tcaccgtcgg gttgatcggc ggcacgatcc ccatggtcgg cacccagctg accgccttgc
     2761 acctgggcac gttcttcccc tggtacctcg tcgctctgat ggtgatctcg acactcttct
     2821 acatcggcat gaaggagacg gcgttcgacc cgctccccga gtaagacgcg aaaaaggtgt
     2881 gtgcggcgcc gcctgccgag gcggtgccgc acacaccttt ttcctgcggg aaagaagctt
     2941 cggcccggcc ggaccggctc cgtcaggcga tgcgggagcg agcgtcgagg gcgtgccagg
     3001 tgcggctgac gtagacgagc ggcgcttccg cctcgggggc ctgcggccag cgcgcctcca
     3061 ggccccgcac ggcgaggact gtcgagttgc ccgcccgcat gcggttcgcg atccggccgc
     3121 ggatccacgt gtgcacggcc ggaaaatacg gctcgcccgt cgccagcctc tcccagatgg
     3181 agtcgtcggc gaaccggtcg atgccggtcg tcgcgccgag cttggcaagg cccagctggt
     3241 cggcggagag caggtgcacg acgagcgtct ccgcacggcc gatggtctct gccgccgacg
     3301 actcgtctga cagggagaag gctaggagcg gcggcaccgc gctgatcgag aacacgctgg
     3361 tcgccgtcaa cgcgaccggc ccgtcgcccg cgtccgccgt gatcagggcc acccccgcgg
     3421 catgccgtcg aaagaccttc ttgaatgcct cggcatcgag gctctcggag gccatggccg
     3481 gctcgtcctc ccaccgatga acttgttcga tcgtcaccgc ccgcctccgt tccctcgtcg
     3541 tcgccgtgtg cgccgtggct cacctggcgg tcttgggcgg gggccgtgac cgcccgccgt
     3601 catcacggcg tggcctcgcg gccgcgtcaa cgccaaccta tgtcgggcct tacccgcgcc
     3661 gcgcgcgggc cgtaacaatg gttaacgcac gccggaccgc ccggcccgca tccggtgcgg
     3721 gttcagccga tggcgcgttc cagcttgtcg gcgatctcgg agacatcgag atagttgtcg
     3781 atgacgacga cgtcggcacg ggtcgggcca agacgcccgg cgagctgtgg tgaggtgagg
     3841 atcacctggg cgtcgcggtg ccgcccggtg acggaggcga ggtcgcccgc ctcgacgacc
     3901 gcgtcgatgt cgagccgttc caaggcacgc tcggcgttga gcttcaggat cgccgaggtg
     3961 ccgattccgg cgccgcagac agccacgatc ttcatcgcac tcccaccttt tcgcgaccgt
     4021 cacggtgcag ggcgccgggg gccacaacga acggacacga cacggacccc gtcgccacgc
     4081 cgaaacctgc cgcgcacgaa cgcaccgcgc ggccgtacgt catgacgagg cctccggacc
     4141 caccgtcata accgcacgca cgtcgtcctt ggtggccgca cgcacgaggc gttcgaccgc
     4201 cccggggtcg ttgaagacgt tcgccagggc ggcgaccccg gtcacatgcg agtcgggcgt
     4261 gcgcagcgcc aggcccacga cgacccgcac ggggtcgtta tggggatgcc cgaactccac
     4321 cgggccctcg agcgtcacga cgctcaaccc gtcggccaga acgtcggggc cgggccgggc
     4381 gtgggcaagc gccagcccgg gggcgatgac gatgtaggcg ccgaactcct cgacgacgtc
     4441 gatcatccgc cgggtgtagc cggggcccgt gcatcccgca cgctcgagcg ccgcacccgc
     4501 gagagacacg gcctcacgcc aaccctccgc gcgggcccgc agcacaatgg ccgggtcacg
     4561 cagcggcggg agatccatat tcgtagcgta ccggccgatt ctgcgcggcc gctgacgccc
     4621 gacccctcat gcaccgcaca taccgtccag ccgccggacg acgggcagcc ggcccgcccg
     4681 gtgtcggtcg tgccaccgcc ggcggggcgg cgcctggcgc agcggccgcc acaccgccgt
     4741 gcgtcgccca tcggccgacg aacccgtagc cgcgccggcg gccgtcacac ggcggcgtag
     4801 gcgctagcca ggcgccgcgc gagctcttcc cgttcgggca gcggcaagaa cgctgcctcg
     4861 gccgcgttcc gttggaaccg ggcgatgtct gagaggccgt aaccgaacgt ctcggccaga
     4921 agcgcgagct cgcgggtgag cgtcgtggcg ctcatcaggc ggttgtcggt gttgaccgtc
     4981 acggtgaagc cttgccggta gaggctgtcg aacggatgct cccggagact cgcgccccac
     5041 ggcgcgatcg cgccggtctg cacgttggag gacgggctca gttcgagcgg cacgcgccgg
     5101 tctcgcaccc accgagcggc cggacccagc gtggcgccgt cggcaccgag gtcttgggcg
     5161 atccgcaccc cgtggccgag ccgcaatgca cggccgtcga cgagcgcccc acggatgctc
     5221 tcgacgccgg ccgcctcgcc ggcatggact gtcacaggaa agaggttctg cgccaggtag
     5281 tcgaaggcct cgcggtggcc ggcgggcggg aaaccgtctt cggggccggc gatgtcgaac
     5341 ccgacgaccc cggcgccccg atggcgcacg gcgagacgcg cggcctccag cgcgttcggg
     5401 cggtgccgca gcgccgagac gagctggccg accctgatct cgtggccccc ggccgccgcg
     5461 cgcgcgacgg ccgcgtcgat gccctcttgc accgcgtcga cggcgtcgtc gggggtgagc
     5521 ccgcgggaca gatgctgctc gggcgcccag cgcacctcgc cgtagacgac cccgtcagtg
     5581 acgaggtcgt cgacgaattc cccggcgacg cgggtcaggg cggccctcgt ctgcatgacg
     5641 gagacggtga cgtcgaaggt gcgcaggtat tcgacgaggc tgccggagtc ggcgtgttcg
     5701 gcgaaccagc gggcaaggcg accggcgtcg tgttccggaa tctcgacgcc gtcctgatcg
     5761 gccagctcga tcaccgtctg cgggcgcagc cccccgtcga ggtggtcgtg cagcgagacc
     5821 ttcggcagcg ggccgagatc cgttccgtca ggcagcagat ggggctcgcg cagtgtctcg
     5881 tcgttcaccg tcttacggta ccgtgcgcca gccccggccc cgtgccccgg ccccggcctg
     5941 cgggcccgac agcggcccgg ctgcttagtg cgtgaagccg ttccggttgt cgaccgtgtc
     6001 gtcgcgctcg tcgagcggcg ggtaaccggc caccagctcg gtgtcgacgt gggtggggcc
     6061 gagcttcgca gcgccacccg gcgaccccag cggctcgacg atgccgggca gcggcttcgc
     6121 gaagaaacgc tcgttgtcgg cgatgaagtc gctccatcga tcgggcacgt cgtcttcgta
     6181 cgagatcgcc tcgaccgggc acacgggctc gcaggcaccg cagtcgatgc attcgtcggg
     6241 gtgaatgtag agcgagcgtt cgccctcgta gatgcagtcg accgggcact cgtcgacgca
     6301 cgcccgatcc atcacgtcga tgcatgccgc accgataaca taggccatgg cgtctcctcc
     6361 ttgcgttcac ccccgcggca cgtggcttct ccatgctatg cgcgccatcc gggcggtcaa
     6421 gcgacaggcc gcgggcgtcg cacttttaga ggatccgaat gcgcttaatt agttatagtc
     6481 gggaacatgc cggtcaccat ctgctccgat agcgaactgg acgtcggcga gttgaagagg
     6541 gtcgtcatcg acggccttcc cgtcgcggtc gtccgggacg acgacggcgg cctgcacgcg
     6601 atcggcgaca cgtgcagcca cgaggagata tcgctctccg agggcttcgt cgagggtcac
     6661 acggtcgaat gctgggcgca cggtgccgtc ttcgacctcg attccggcga cgcgctgtcg
     6721 ctgccggcca ccgagcccgt gccggtcttc gcggtctggg tggccgacgg gcagatcgtc
     6781 gtcgacaccg gggcatcgcc gggcgacgcg ggccccgacg cccggaacac gcccgacacg
     6841 acccccgacg actcgaacga ccagccagag aaagcacccg ccatgacgga gcccgacgcc
     6901 gaactgatcg agaaggtcga agaggccctg aaagacgtga tcgaccccga actgggcgtc
     6961 gacatcgtca acctgggtct cgtgtacggc tacacgtggg atgcggccaa ccaacgcctc
     7021 ttcatcaagg caaccttgac ctccgccgaa tgcggccacc aagggatcat cggcagccag
     7081 atcgcgaagg cgatcgaagg catcgtgccc tcgcacgtcg tcgagtgggt ctggatgccg
     7141 gcgtggagcc ccgcgcgggt caccgaagac ggccgcgaca tgatgctggc gctgggcttc
     7201 aatgtctgac gagcagcacc cgtcagccga agctccagag ccgcggggcg ccgggccgaa
     7261 gacgggtcgc agcggcatcg ccgcagacga gacgagcgcg tacggctacc ttgtgccgcg
     7321 catccacggt gcgctgacac aggtcatcga cccagagatc cggcggccca tcaccgagtt
     7381 gaacatggtc cccggcatcg aggtcgacga ccaggccgac gcgaccgtca cgatccgcct
     7441 gacgacggcg acctgcccga actcgcg
//
LOCUS       DASTNL010000165        18140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_667681, whole genome shotgun sequence.
ACCESSION   DASTNL010000165 DASTNL010000000
VERSION     DASTNL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 18140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18140
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667681"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFQ96_05120"
     CDS             complement(<1..1011)
                     /locus_tag="VFQ96_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEU0257204.1"
                     /translation="MALFVRHPAGDAPKLAHRAARIEDRLHRFLSRRARRHGHVTTIV
                     AYTGYGDTHRVRILARVLLVKEPRQGSRAERRQRGEHVRGWRAFLSVPVSDARVTITI
                     GGRSCTVHPDRGGVVDHTLAIDLDPGWHTATLSTQAATKTWPAPVFVVDPRADFGIVS
                     DVDDTVMVTALPRPFLAAWNTFVVDEHARVPTPGIAVMLERLVAEHAGGPVVYVSTGA
                     WNVAPAVTRFLSRNLYPPGPLLLTDWGPTHDRFFRSGTEHKRQSLERLHEEFPQMRWL
                     LLGDDGQHDEQTYDEFALRHPDNVAAVAIRQLSPSEAVLAGRRKKTPRRTVRSHVPWV
                     AAP"
     gene            1247..2074
                     /locus_tag="VFQ96_05125"
     CDS             1247..2074
                     /locus_tag="VFQ96_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257205.1"
                     /translation="MIEFRSVTKRYAGGTLAVSDFDMVAASHRTTVLVGSSGSGKTTI
                     LRMINRMVEPTSGTVLIDGQDVAGMDPVRLRRSIGYVMQNSGLLPHRTVLQNIATVPR
                     LNGVSRSEAARRARGLMQTVGLDESLAGRYPAQLSGGQQQRVGVARGLASDPNILLMD
                     EPFGAVDPLVRTELQQELLRLQSQLGKTVVFVTHDIDEALLLGEQLIIFRVGGRVAQR
                     GTPAEILAHPADEFVSDFVGVTRGKRSLTLAPDGRVLLDAAGRPAGVFAGHGDGRVP"
     gene            2071..2769
                     /locus_tag="VFQ96_05130"
     CDS             2071..2769
                     /locus_tag="VFQ96_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0257206.1"
                     /translation="MIWLQQNAGMLWGLTLVHAGLSIPPIILGFVVSLPLGWLAYRFR
                     VPRHAKIAFGGVVLTAGGLLYAIPSLALFMVLPGILGTSILDPVNVEVALTLYAIALM
                     VRTTADALASVEPDVRDSSKALGLSGWQSFWRVELPLAGGPLLAGLRVVSVSTVSLLS
                     VAAVIGVNTLGYLFLDGLQRDFPLEVSVGIVGSVVVAAVFDAVLVLVGRLAMPWQKAL
                     RESRLTGVGEAAAS"
     gene            2766..3458
                     /locus_tag="VFQ96_05135"
     CDS             2766..3458
                     /locus_tag="VFQ96_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0257207.1"
                     /translation="MIWFANAFAWLADPAHYAGVNGIPVRLGQHVAYTGITLVIAAAV
                     ALPLGFLIGHTGKGRSLAVQVSGGLRSLPTLGLVIVLALLLGLGIGAPMIALVILAVP
                     PVLAGAYSGVESVDPRVVDAARAVGMTGWQVLWRVEVPLGLPLVIGGLRSAALQAIAT
                     WTVAAVLPLGGLGRYIYDAIATYDYTEMFAGSVLVIVLALVVDGLFAVLQRLVVPRGV
                     VAAGGAPAAVPA"
     gene            3539..4474
                     /locus_tag="VFQ96_05140"
     CDS             3539..4474
                     /locus_tag="VFQ96_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0257208.1"
                     /translation="MRFRRKTSLAAVAAAGALVALVLAGCSTSNPTASPSSSAAAAKS
                     SQEPIVVGSAAFPENEILAEIYAQALTGAGIPATTHLDIGQRPAYLKAMKEGSIDLVP
                     EYSGNLLQFYNKDTTDKSSAGVFAALQKAIPSSLEVLPEAEAQDRDSYNVTKEFAKKY
                     GLVSLGDLTKAPQPLIVGGNPELGQRPYGLPGLKSVYGVTSTLKPISDSGGPLTIKAL
                     QDGTIQVADIFTTTPAITQFVTLKDPKNMVLAQNIVPLIRKSKASAKVKSTLDAVSAK
                     LTTKALIAMNGKSSGSAKESSAQIAKEWLSQEGLD"
     gene            4599..7124
                     /gene="leuS"
                     /locus_tag="VFQ96_05145"
     CDS             4599..7124
                     /gene="leuS"
                     /locus_tag="VFQ96_05145"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU0257209.1"
                     /translation="MAEGSRTTDEARYDFAAIQDRWLPVWDRLRPFNTANAPTGAARK
                     YVLDMFPYPSGDLHMGHAEAYALGDVIARYWRHRGFQVLHPIGWDAFGLPAENAAIKR
                     GLDPRGWTHDNIAQQRASMRRYAPSFDWDRVIQTCEPEYYRWNQWLFLRLYEKGLAYR
                     KASLVNWCPFDQTVLANEQVVGGRCERCDTIVTKKKLTQWYFRITEYADRLVDDLAGL
                     EGSWPAKVVSMQRNWIGRSTGADVHFAVEGRDEPIVVYTTRPDTLYGATFMVVAPDSD
                     LAEELAAGASATVQAEFAAYLEKVRGMSEMERMNAGREKTGVFLGRYATNPLTGERLP
                     IWAADYVLADYGHGAIMAVPAHDQRDFDFARRFDLPIRIVVDPTRGGTTGGAPLAERD
                     PRVTGEALVGEGTLVASGDFTGLGKAEAIAAVTAWLAERGLGGPTTNFRLRDWLISRQ
                     RYWGTPIPIIHCAACGEVPVPDDQLPVVLPDAAGLDLQPRGTSPLGAADAWASVACPR
                     CGGPARRDPDTMDTFVDSSWYFLRFLSPHDDTQAFDRAEVERWAPVDQYVGGVTHAIL
                     HLLYARFITKVLHDLGYVGFSEPFSALLNQGMVLMDGSAMSKSRGNLVRLSQQLDEHG
                     VDAVRLTMAFAGPPEDDIDWADVSPAGSAKFLARAWRLAGAVESDPDGEAALGDTALR
                     RATHRLLADVGPLVESFKFNVVVARLMDLVNTIRHRMDAGVASADPALREAVEALALV
                     LDMFAPYTAEDMWQRLGHEPSVALAPFPAADDALLVEETVTAIVQVDGKLRDRLQVAA
                     TIDGAALEALARASEAALRAVGERRIVRVIVRAPRMVNLVTAP"
     gene            7280..7954
                     /locus_tag="VFQ96_05150"
     CDS             7280..7954
                     /locus_tag="VFQ96_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU0257210.1"
                     /translation="MATTKPAPASFDALDPKTRIPRLRLATRLVAALLFLVAAAVAAT
                     VVVAVSARPGRMTVEAAPSLPPTRAAAQATSPPGQDVVFVHVDGAVRTPGLYRLQKGS
                     RVVDAVAAAGGFTAKAQRSAANLARVLSDGEQILIPQKGEPLSGAGGPAGPGGAGPAV
                     GAPVNLNTATGDELETLPRVGPALAGRILSWRAEHGRFERIDDLMSVSGIGEKTFAGL
                     RDRVTV"
     gene            7951..10311
                     /locus_tag="VFQ96_05155"
     CDS             7951..10311
                     /locus_tag="VFQ96_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU0257211.1"
                     /translation="MTAARRAPLDLRLAAAAAGAWAVAWIVTAFPDAAAAACLVAFLV
                     AATLAAMSFRRRRPTSILATVVLTAAAAGLVAASIAGQAGPRHPAGITAAADGGRVLT
                     ADLVVVTPPRGEAGRFGAPQETFRAVVRAVHRGETIVTGRAPALVKIPVDVTAEAGAG
                     QRAASVVRPGARLRVSARFFSHPREHSTAFTVVATDEPVLLRAPGRWIAWADPLRARF
                     LALAGALPGDGGDLLPGLSIGDTRAVSEGLDAAMKASSLSHLTAVSGANCAVVVTLVS
                     ALAAACGADRRARAGLAVGALVGFLVLVTPGASVTRAALMAVAALAADLTGRGGGGLP
                     FLALAVLVMVTADPWISRDYGFALSVLATAGLIVLAPPLAGRLGRILPARLARVVAVP
                     LAAQVFCQPVLVLLAPQLPVYGVAANVLADLPATIATILGLASCLLLPVLPGLAQGVA
                     QLAWLPSAWIAATARLAAGLPAASLPWLPGLAGLGVAAAGVAVVIVLVFTPPGSGTRA
                     SLVAMAAGVVLVGGYAGAGTVTMLAAAAHPRDWLVAACDVGQGDGLVLQSGGRHAVID
                     VGPEPAAMARCLRALGVRHVDLAVITHFDLDHVGGLDAILGKTGVALVGPARSEAARK
                     VSERLAASGTDLRQARAGDHGTLGDLDWKVLWPTTETAGMTGGNELSVTLQVSQAGLR
                     AVFLGDLDEKAQQALLAGGIEPVDIVKVAHHGSADQSSALYEALHARVGIISVGADNT
                     YGHPTPRLLGILQKAGTAAVRTDTGGMLLVGPAPGGRLSVWSETAP"
     gene            10362..11396
                     /locus_tag="VFQ96_05160"
     CDS             10362..11396
                     /locus_tag="VFQ96_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU0257212.1"
                     /translation="MASRGTSRRSARAAEVKAAIPRLSWAKARPAPVVLVSGRENVLA
                     DRAIAHIKEALLATDPNLEVNDIDAAGYGAGELLTLASPSLFADPRLIRVEAVEAASD
                     PFLTDALAYLAAPADDTVLVLRHHGGTRGKRLLDRVRAGLGGGVEVVCAEVTRDRDRA
                     DFARAEFGRAGREATPGAARALVDAFNDLAGLAAACSQLVSDTEGLVDEGVVRRYYAG
                     RVVANGFAVADQALAGRAGLALVTLRQAWESGTDPVPIVAALASKIRAMARVAALRGT
                     DAAVARQAGMAPWQVERARRDLRGWGDTSLARAILAVADADAAVKGAARDPKYAVEKA
                     VRLIAREARG"
     gene            complement(11393..11653)
                     /gene="rpsT"
                     /locus_tag="VFQ96_05165"
     CDS             complement(11393..11653)
                     /gene="rpsT"
                     /locus_tag="VFQ96_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU0257213.1"
                     /translation="MANIKSQIKRVGTNRKAQERNKAVKSELRTALRATREAVAAGDK
                     EKATSALSVAAKKLDKAASKGVIHKNQAANRKSAIASQVAAL"
     gene            11839..13698
                     /gene="lepA"
                     /locus_tag="VFQ96_05170"
     CDS             11839..13698
                     /gene="lepA"
                     /locus_tag="VFQ96_05170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU0257214.1"
                     /translation="MATRAESAPRPAATDPALIRNFCIIAHIDHGKSTLADRMLQLTG
                     SVSERDMRAQYLDRMDIERERGITIKSQAVRMPWQAGGRGYVLNMIDTPGHVDFSYEV
                     SRSLAACEGAVLLVDASQGIEAQTLANLYLALDHDLTILPVLNKIDLPSADPDRYARE
                     LSGLIGGDPADVMRVSAKTGEGVTALLDRLVERVPPPAGERQEASRALIFDSVYDSYR
                     GVVTYVRMVDGVLHARDRIRMMSTGATHEILEIGVASPEPTPTQGLAVGEVGYLITGV
                     KDVRQSKVGDTVTDASNPAAEALAGYTEPLPMVFSGLYPLDGSDYPDLRDALDKLKLS
                     DAALAYEPETSVALGFGFRCGFLGLLHLEIVTERLQREFGLDLIATAPSVTYHVTAED
                     GTELTVTNPSEFPPGRLVSVREPVVRAAILAPKEYVGVIMELCQGKRGTLEGLDYLSE
                     NRVEIGYTMPLGEIVFDFFDQLKSKTAGYASLNYEPAGEAEADLVKVDILLQGERVDA
                     FSAIVHRDKAYDYGVLMTGRLKKLIPRQQFEVPIQAAIGARIIARETIRAMRKDVLAK
                     CYGGDITRKRKLLEKQKEGKKRMKTLGRVDVPQEAFIAALSGDVDTRNDQRKK"
     gene            13702..14946
                     /gene="hemW"
                     /locus_tag="VFQ96_05175"
     CDS             13702..14946
                     /gene="hemW"
                     /locus_tag="VFQ96_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU0257215.1"
                     /translation="MPAPLPQGEPAPEDGLLPPTAREGAARRAFGVYLHVPFCRTRCG
                     YCDFNTYTATELQGAKQEDYAAEAAREIRFAGRALRRSGVPGRLAQTVFFGGGTPTLL
                     PSDHLARMLGTVREVWGIAPGAEITTESNPDSVDERYLAELAEAGFTRVSFGMQSAVP
                     HVLKTLERTHDPARIPLVVRWARRAGLDVSLDLIYGTPGESLDDWRCSLDAALEQEPD
                     HISAYSLIVEPGTKLARQVRRGLVPEPDDDLAADEYELADEVLGRAGFDWYEISNWAR
                     RAPGETGRAMAHRSRHNLAYWRDADWWGIGPGAHSHVGGVRWWNVKHPAAYAHRVQAG
                     LSPAAGREVLDEATRTMEAIMVGVRIAEGFPVARLGEAARSEVPGLVAEGLLEPEEAA
                     RGMLVLTLRGRLLGDAVVRRLT"
     gene            15072..16097
                     /gene="hrcA"
                     /locus_tag="VFQ96_05180"
     CDS             15072..16097
                     /gene="hrcA"
                     /locus_tag="VFQ96_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU0257216.1"
                     /translation="MISERSLDVLRVIVQDYVANNEPVGSKTIVERHDFGVSAATIRN
                     DMASLEEEDLIVAPHTSSGRVPTDKGYRLFVDRLATVRPLSPAQRLAIERFLGESVDV
                     DGVLERTVRLLSQLTHQVALVQYPSAAHSAIRRIEFVSLGPNKVLCVLIAAGGVVEQR
                     VVDLPRDAGEDWLGVVQDRVNGAVHGLEVGDALARLAELAEATGGSDRDAGLALVEAV
                     REQLAVERHDRLVVAGSSNLIRGERDFNGNISPVLDALEEQVVLLRLFSEMEEDRRGV
                     SASIGRENASFGLGEASVLSASYTGTGGRAAHLGVLGPTRMDYTANMAAVRAVARYLS
                     RLFGENI"
     gene            16120..17223
                     /gene="dnaJ"
                     /locus_tag="VFQ96_05185"
     CDS             16120..17223
                     /gene="dnaJ"
                     /locus_tag="VFQ96_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU0257217.1"
                     /translation="MADHYEILGVERDATPDEIKKAYRKLARRLHPDVNPSPDAAERF
                     KLVTQAYDTLSDPERRRRYDMGGEEHNFAGQGFGGFSDIFETFFGGQGAGRGPRSRRE
                     NGQDALLHIDVELADVIFGTHRDIEVDTAVVCETCGGSCCQPGTAPVTCDICHGTGHV
                     QRTVRSLLGNVVTSTPCGTCRGYGTIIATPCSTCQGRGRVRAHKTIPVDIPSGVETGL
                     RLQMPGSGEAGPGGGANGDLYLEITVRNHDVFSRDGDDLLCTLDVEMSDAILGTKTTL
                     QTLDGDVDVEVRPGTQSSEVITVQGRGITHLRGSGRGDLRIGVHVVTPTKLDHKDRDL
                     IKRFAEGRKQQPPRLARAGQGVFQRLRDRFMRV"
     gene            17230..18033
                     /locus_tag="VFQ96_05190"
     CDS             17230..18033
                     /locus_tag="VFQ96_05190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU0257218.1"
                     /translation="MASLFYDESLADTGEGLPEVGAVCRLSGAEAHHAAVNRLRPGET
                     VSVGDGAGTIVTGPVVTLDREGLAVRVERRVRKPRPAPRLGLAQALAKGGRDELAVQA
                     ATELGVDVVVPWMARRSVARWSGQKRDRGPERWRAIVREAGKQSLRAWLPEVLPPHST
                     AELAGLAGVWQLAVLDPASNLTMTAWLGTGPRAQGGGLSAAGGGAAGPDRRDVLLVVG
                     PEGGIAADEARLLEEAGAARLRLGQTVLRTSTAGPAAIAVWNACGGRRW"
BASE COUNT         2587 a         6490 c         6297 g         2766 t
ORIGIN      
        1 gggtgcggcc acccacggaa cgtgcgaacg caccgttcga cgaggtgtct tcttgcgtcg
       61 gccggccagg acggcttcac tcggcgagag ctggcggatc gccactgctg cgacattgtc
      121 cgggtgacgc agcgcgaact cgtcataggt ctgctcatcg tgctggccgt cgtcgccgag
      181 caggagccag cgcatctgcg ggaactcctc gtgaaggcgt tcgaggcttt gccgcttgtg
      241 ttcggtgccg ctgcggaaga accggtcgtg ggtcggaccc cagtccgtga ggaggagagg
      301 accgggcggg tagaggttgc gcgagaggaa ccgtgtcacc gccggcgcca cgttccacgc
      361 cccggtcgag acgtagacga ccggtccgcc ggcatgttcg gcgacgaggc gttccagcat
      421 gaccgcgatg cctggcgtgg gcacccgcgc gtgctcgtcg accacgaagg tgttccatgc
      481 agccaggaac gggcgcggca gggccgtcac catgaccgtg tcgtccacgt cggagacgat
      541 gccgaagtcg gcgcgggggt cgacgacgaa gaccggcgcg ggccaggtct tggtggcggc
      601 ctgtgtggag agcgtcgcgg tgtgccagcc ggggtccagg tcgatcgcca gcgtatggtc
      661 gacgacgccg ccgcggtcgg gatgcacggt gcagctgcgg ccgccgatcg tgatcgtcac
      721 ccgggcgtcg ctgaccggca cgctgaggaa cgcgcgccag ccccgcacgt gctcgccgcg
      781 ctggcgtcgc tccgcgcggg aaccctggcg cggctccttc acgagcagca cccgggcgag
      841 gatccgcacc cggtgggtgt cgccatagcc ggtgtaggcg acgatcgtcg tcacatggcc
      901 gtgtcgccgg gcccgcctgg agaggaatcg gtgcaggcgg tcttcgatgc gggccgcgcg
      961 gtgcgccagt tttggcgcgt cgccggccgg atgtcgcacg aagagggcca tcgaaccagt
     1021 ctcgcacgcg gtcccgccgc atctggccgg ccgggcgcgg tgtgccggtc tggaatatgt
     1081 caggcccggg cgccgctaca cagggtgaga tgtgtcgtgg ccgccccggc ctgcggcgat
     1141 cggcacggac ccgctggtat ttgccatccg ggcgaagcgg tgtttggctt gagacggtgg
     1201 atacacgtca cggcacgcca atgctcgacg gatgggaacc acaagcatga tcgaattccg
     1261 gtcggtcacc aaacgctacg ccggcggcac cctcgccgtc tctgacttcg acatggtcgc
     1321 cgcctcgcat cgtacgacgg tgctcgtcgg ctcctccggc agcggcaaga cgacgattct
     1381 gcgcatgatc aaccgcatgg tcgagcccac ctccggcacc gtcctcatcg acggccagga
     1441 cgtggccggc atggaccccg tgcggctgcg gcgcagcatc ggctacgtga tgcagaattc
     1501 cggcctcctg ccgcaccgca cggtgctgca gaacatcgcc accgtgccgc gcctgaacgg
     1561 tgtctcgaga agtgaggcgg cccggcgggc acggggcctg atgcagacgg tcggcctcga
     1621 cgaatccctt gccggccggt accccgcaca gctttcgggg ggccagcagc aacgggtcgg
     1681 cgtggcacgc ggcctcgcga gcgatccgaa catcctcctc atggatgagc cgttcggcgc
     1741 ggtcgacccg ctggtgcgca ccgaattgca gcaggagctt ctacgcctgc agtcgcagct
     1801 gggcaaaacg gtcgtctttg tcacccacga catcgacgag gcgctgcttc tgggggagca
     1861 gctcatcatc ttccgggtcg gcggccgggt cgcgcaacgc ggcacgcctg ccgagatcct
     1921 cgcgcacccc gccgacgagt tcgtctccga cttcgtcggg gtgacgcgcg gcaagcggtc
     1981 tctcacgctg gcgcccgacg gccgggtgct tctcgacgcg gccggccggc cggccggcgt
     2041 cttcgccggg cacggggatg gccgcgttcc gtgatctggc tgcagcagaa cgccggcatg
     2101 ctgtgggggc tgactctcgt acacgccggc ctcagcattc cgcccatcat tctcggcttc
     2161 gtcgtctcgc tgccgttggg gtggctcgcg taccggttcc gggtgccgcg gcacgccaag
     2221 atcgcgttcg gcggagtcgt cttgaccgcg ggcgggttgc tctacgccat cccgtcgctt
     2281 gcgctcttca tggtgctgcc gggaatcctc ggcacgagca ttctcgatcc ggtgaacgtc
     2341 gaggtcgcgc tcaccctcta cgccatcgcc ctcatggtgc gcaccacggc cgacgcgctc
     2401 gcctccgtcg aacccgacgt gcgtgactcg tcgaaggcgc tcgggctctc cggctggcag
     2461 agtttctggc gggtggaact gccgttggcc ggcgggccgc ttctcgcagg gcttcgcgtc
     2521 gtctcggtca gcaccgtcag cctgctgagc gtcgccgccg tgatcggcgt caacacgttg
     2581 ggttacctct tcttagacgg cctgcaacgt gactttccac tcgaggtgag cgtaggcatc
     2641 gtcggctccg tcgtcgtcgc ggccgtcttc gacgccgtgc tcgtgctcgt cggccggctg
     2701 gcgatgccgt ggcagaaggc cctccgcgag tcccgcctca ccggcgtggg cgaggcggcc
     2761 gcctcatgat ctggttcgcg aacgccttcg cctggctcgc cgaccccgcc cactatgcgg
     2821 gcgtcaacgg catcccggtg cgcctcggtc agcacgtcgc ctacacgggg atcacgctcg
     2881 tgatcgccgc ggcggtcgcg ctgccgctcg gcttcctgat cggccacacc ggcaaggggc
     2941 gctcactcgc cgtgcaggtc tccggcgggt tgcgctccct gccgacgctc ggcctcgtca
     3001 tcgtcctcgc cctgctcctc ggcctgggga tcggtgcgcc gatgatcgcc ctcgtcatct
     3061 tggcggtgcc gccggtgctc gctggggcgt attccggcgt ggagtccgtc gaccctcggg
     3121 tcgtggacgc cgctcgcgcc gtgggcatga cgggctggca ggtgctgtgg cgggtcgagg
     3181 tgccgctcgg cctgccgctc gttatcggcg ggctgcgctc cgccgccctg caggcgatcg
     3241 cgacctggac cgtggcggcc gtgctgccgt tgggcgggct cgggcgatac atttacgacg
     3301 cgatcgcgac ctacgactac accgagatgt tcgccggttc tgtgcttgtc atcgtcttgg
     3361 cgctcgttgt cgacgggctg ttcgccgttc tgcagcgtct cgtcgtgcct cgcggcgtcg
     3421 tcgcggcagg cggggccccc gccgccgtac cggcgtgagc ggccgccggc agagagaaaa
     3481 gacccccacc ccccaccaga gagagcaccg cgaaggcggt gcggagaaag gaacagcaat
     3541 gagattccgt cgaaagacaa gccttgctgc ggtcgcggcc gccggagcat tggtggcgtt
     3601 ggttctcgcc ggctgctcca ccagcaaccc gaccgcgtcg ccctcctctt ccgccgcggc
     3661 cgccaagagc tcccaggagc cgatcgtcgt cggctcggcc gcgttccccg agaacgagat
     3721 cctcgccgag atctacgcgc aggcgctcac gggtgccggc atcccggcga cgacccacct
     3781 cgacatcggg cagcggcccg cctacctgaa ggcgatgaag gagggctcga tcgacctggt
     3841 gcccgaatac agcggaaacc tgctgcagtt ctacaacaag gacaccaccg acaagagcag
     3901 cgccggcgtc ttcgccgccc tgcagaaggc gatcccctcg agcctcgagg tcctccccga
     3961 ggcggaggcg caagatcggg actcctacaa cgtcacgaag gagttcgcga agaagtacgg
     4021 gctcgtgagc ctcggcgacc tcaccaaggc cccgcagccg ctcatcgtcg gcggcaaccc
     4081 ggagctcggg caacggccct acggtcttcc gggcctgaag tcggtgtacg gcgtcacctc
     4141 gacgctgaag ccgatcagtg acagcggcgg ccccctcacc atcaaggcgt tgcaagacgg
     4201 cacgatacag gtggccgaca tcttcaccac gacgccggcg atcacccagt tcgtgacgtt
     4261 gaaggacccg aagaacatgg tcctcgcaca gaacatcgtg ccgctcatca ggaagagcaa
     4321 ggcaagcgcc aaggtgaaga gcaccctcga cgcggtttcc gccaagctca ccaccaaggc
     4381 gctcatcgct atgaacggca agagcagcgg ttccgccaag gagtcttcgg cccagatcgc
     4441 gaaggagtgg ctcagccaag aggggctcga ctgagccact aacctggatg ccggggcgcc
     4501 gcaccccggc atccggagcc ggcggtgcac ggatgcggct ttcgctgcat ccgcaccgcc
     4561 ccacatcgtc tgaaaggatc gggaaagacg tgaccgagat ggccgaggga tcgcgcacga
     4621 ccgacgaggc acgctacgac ttcgctgcga tccaagatcg ctggctgccg gtgtgggacc
     4681 gtctgcggcc gttcaacacg gcgaacgctc ccacaggtgc ggcacgcaaa tacgtgctcg
     4741 acatgttccc ctacccctcc ggagacctgc acatgggtca cgcggaggcg tacgccttgg
     4801 gcgatgtcat cgcccgctac tggcggcacc gcggctttca ggttctgcac ccgatcggct
     4861 gggacgcgtt cggcctgccc gccgagaacg cggccatcaa gcgcggcctc gacccgcgcg
     4921 gctggacgca cgacaacatc gcccaacagc gtgccagcat gcgccgctac gcaccgagct
     4981 tcgactggga ccgggtcatc cagacgtgcg agcccgaata ttatcgctgg aaccagtggc
     5041 tgttcctgcg gctgtatgag aagggcctcg cctaccgcaa ggcgagcctc gtcaactggt
     5101 gcccgttcga ccagacggtg ctcgccaacg agcaggtcgt cggcggccgc tgcgaacgct
     5161 gcgacacgat cgtcaccaag aagaagctca cccagtggta cttccgcatc acggaatacg
     5221 ccgaccggct ggttgacgac cttgcggggc tggagggctc ctggcccgcc aaggtcgtca
     5281 gcatgcaacg caactggatc ggccgctcca cgggcgccga cgtgcacttc gcggtcgagg
     5341 gccgagacga accgatcgtc gtctacacga cccgacccga cacgctgtac ggcgccactt
     5401 tcatggtcgt cgcccccgac tccgacctcg ccgaagagct tgccgcgggg gcctctgcca
     5461 ccgtgcaggc cgagttcgcc gcctacctcg agaaggtgcg tggcatgagt gagatggagc
     5521 ggatgaacgc cggccgcgag aagaccggcg tcttcctcgg ccgctatgcg acgaacccgt
     5581 tgaccggcga acggctgccc atctgggccg ccgattatgt gctggccgac tacggtcacg
     5641 gcgcgatcat ggcggtgcct gcccacgacc agcgcgactt cgacttcgcc cgccgcttcg
     5701 acctgccgat ccgaatcgtc gtcgacccga cccgcggcgg aacgacgggc ggcgccccgc
     5761 tcgccgagcg cgacccgcgg gtcacgggtg aagccctcgt cggcgagggc accctcgtcg
     5821 cgtcgggcga cttcaccggc ctcggcaagg cggaggccat cgcggcggtc accgcgtggc
     5881 tcgccgagcg gggcctgggg gggccgacca ccaacttccg gctgcgcgac tggctcatct
     5941 cgcgccagcg ttattggggc acgcccatcc cgatcatcca ttgcgccgcg tgcggcgagg
     6001 tcccggtgcc cgacgaccag ctgcccgtcg tcttgcccga tgccgcgggg ctcgacttgc
     6061 agcctcgcgg caccagcccg ctcggcgcgg cggacgcgtg ggcgagcgtc gcctgcccgc
     6121 gctgcggcgg gccggcccgc cgcgatcccg acacgatgga cacgttcgtc gacagctctt
     6181 ggtatttctt gcgctttctc tcgccgcacg acgacacgca ggcgttcgac cgcgccgagg
     6241 tcgaacggtg ggcgcccgtc gaccagtacg tcggtggggt gacgcacgcc atcttgcatc
     6301 tgctctacgc ccgcttcatc acgaaggtgc tccacgacct tggctacgtg ggcttctccg
     6361 agcctttctc cgccctcctc aaccaaggca tggtgttgat ggatggctct gcgatgagca
     6421 agtcgcgcgg caacctggtg cggctctcgc aacagcttga cgagcacggc gtcgacgcgg
     6481 tgcgcctgac gatggcgttc gcgggcccgc ccgaagacga catcgactgg gcggatgtct
     6541 cgccggccgg cagcgccaag ttcctcgccc gcgcgtggcg gttggccggc gcggtcgaga
     6601 gtgaccccga cggcgaggcg gccctaggcg acacggcgtt gcggcgcgcc acgcaccggc
     6661 ttctcgccga cgtcggcccg cttgtggaat cgttcaaatt caacgtcgtc gtcgcacgcc
     6721 tcatggatct cgtgaacacg atccgccacc ggatggacgc cggggtggca agcgccgacc
     6781 ccgccctgcg cgaggccgtc gaggcgctcg cgctcgtcct cgacatgttc gccccttaca
     6841 cggcggaaga catgtggcag cggctcggcc acgagccttc cgtcgcgctg gcgcccttcc
     6901 cggccgccga cgacgcgctg ctcgtcgagg agaccgtcac cgccatcgtg caggtcgacg
     6961 gcaagctgcg cgaccgcctg caggttgccg cgacgatcga cggcgctgcg ctggaggcgc
     7021 tcgcccgggc cagcgaggcg gccctgcgtg cggtcggcga gcgccgcatc gtgcgcgtca
     7081 tcgtgcgggc gccgcgcatg gtgaacctcg tcaccgcgcc gtaacctttc gtccccgggc
     7141 gcgtttcgac tccgggctgg tgcggctccg gcgcaggcag cggggtcgcg gctccggcac
     7201 cgagctcgtc cgggcggcgt cctccacagt atgccgcggt gccggggctc gggaggtcgg
     7261 gccgacttag cgtggccgcg tggccaccac gaagcccgcc ccggcgtcgt tcgacgcact
     7321 cgaccccaag acccggatac cgcgcctgcg cctggccacc cggctcgtcg cggcgttgct
     7381 gttccttgtt gcggcagccg tcgccgcgac ggtcgtcgtc gccgtctccg cccggccggg
     7441 gcggatgacc gtcgaggccg cgccgagcct cccgccgacc cgtgccgcag cgcaggctac
     7501 ctccccgccg gggcaagacg tcgtgttcgt ccacgtcgac ggtgccgtgc gcacccccgg
     7561 gttgtaccgg ctgcagaagg gctcccgggt cgtcgacgcc gtcgcagcgg ccggcgggtt
     7621 caccgcgaag gcgcaacgct cggcggcgaa ccttgcccgt gtgctctccg atggggagca
     7681 gattctcatt ccccagaagg gagaaccgct ttccggcgcc ggcgggccgg ccgggccggg
     7741 cggcgccggg ccggcggtgg gtgcaccggt caacctgaac acggcgaccg gcgacgaact
     7801 ggagacgcta ccgcgggtcg gcccggcact ggccgggcgc atccttagct ggcgggccga
     7861 acacggcagg ttcgagcgga tcgacgacct catgagcgtc tccggcatcg gtgagaagac
     7921 tttcgcgggg ctgcgcgacc gggtcaccgt atgacggccg cccggcgcgc cccgctcgac
     7981 ttgcggctcg ccgcggcggc ggccggcgcc tgggccgtgg cctggatcgt gaccgcattt
     8041 cccgatgccg ctgcggccgc ctgcctggtc gcgttccttg tggcggcgac gctcgcggcc
     8101 atgtcgttcc ggcggcgccg gccgacgagc attcttgcca ccgtcgtgct cacggcagcc
     8161 gccgccgggc tcgtcgcggc gagcatcgcg ggccaggccg ggccgcgtca cccggcgggg
     8221 atcacggcgg ccgccgacgg cggccgggtg ctcaccgccg acctcgtcgt cgtcacgccg
     8281 ccccgcggcg aggcgggaag gttcggcgcc ccacaagaga cattccgtgc cgtcgttcgc
     8341 gccgtgcacc gcggcgagac gatcgtcacc ggccgcgctc cggcgctcgt gaagattccg
     8401 gtcgacgtca cagccgaggc cggtgccggg cagcgagccg cctccgtcgt tcgccccggc
     8461 gcacgcctgc gggtgtcggc gaggttcttc tcccacccgc gggagcactc caccgcgttc
     8521 accgtcgtcg ccaccgacga gcccgtcctc cttcgcgcgc ccggccgctg gatcgcctgg
     8581 gccgacccgc tgcgggcacg cttcctggcg ctcgcggggg cgctgcccgg cgatggcggc
     8641 gaccttctgc ccggcctttc gatcggcgac acgcgcgctg tctccgaggg gctcgacgcg
     8701 gccatgaagg cctccagcct cagccacctg acggccgtct ccggtgcgaa ctgcgccgtg
     8761 gtggtcacgc tcgtctccgc ccttgctgcg gcgtgcggcg ccgatcgccg ggcgcgagcc
     8821 gggctggccg tgggtgccct ggtcggtttc ctcgtccttg tcactccggg cgccagcgtg
     8881 acccgggccg cactcatggc ggtggccgcg ctcgccgccg acctcaccgg ccgcggcggt
     8941 ggggggctcc cgttcctggc gctcgccgta ctcgtcatgg tgacggctga cccgtggatc
     9001 agccgggact acggcttcgc tctgtctgtg ctcgcgacgg cgggtctcat cgtgctcgcc
     9061 ccgccgctcg ccggccggct gggcaggatt ctgccggccc gcctcgcccg ggtcgtcgcg
     9121 gtaccccttg cggcgcaggt cttctgccag ccggtgctcg tccttctcgc gccacagctg
     9181 ccggtatacg gcgtcgccgc gaacgtcctc gccgacctgc ccgccacgat cgcgacgatc
     9241 ctcggcctcg cctcctgcct gctcttgccg gttctccccg gcctcgcaca gggcgtcgca
     9301 cagctggcat ggctgccttc ggcctggatc gcggccaccg cccgcctggc ggccggcctg
     9361 cccgctgcga gcctgccgtg gttgccgggc cttgccggcc tcggggtggc ggcggccggc
     9421 gttgcggtcg tcatcgtgct ggtcttcacc ccgccgggca gcggcacgag ggcgagcctc
     9481 gtcgcgatgg cggccggcgt ggtgctcgtc ggcggctacg ccggggccgg caccgtgacg
     9541 atgctggccg ccgccgcaca tccacgcgac tggctcgtcg cggcctgcga cgtcggccaa
     9601 ggcgacgggc tcgtcctgca aagcggtggg cgtcatgcgg tgatcgacgt cggcccggag
     9661 ccggccgcga tggcccgttg cctgcgcgcc ctcggtgtgc gccacgtcga cctcgccgtc
     9721 atcacgcact tcgacctcga tcatgtcggc ggcctcgacg cgatcctcgg caagaccggc
     9781 gtcgccctgg tcggcccggc acgaagcgag gccgcacgca aggtctcgga gcgcctggcc
     9841 gcctcaggca ccgacctgcg ccaggcacgt gccggcgacc acggcaccct cggcgacctg
     9901 gactggaagg tcctctggcc gacgaccgag accgcgggca tgaccggcgg caatgagctt
     9961 tccgtcacgc tgcaggtctc ccaggcaggc ctgcgcgccg tcttcctcgg cgacctcgac
    10021 gaaaaggccc agcaggcgct cctcgccggc ggcatcgaac cggtcgatat cgtcaaggtc
    10081 gcccaccacg gctccgccga ccaaagttcg gcgctctacg aggcgcttca cgcccgggtc
    10141 ggcatcatct cggtgggggc cgacaacacg tacgggcacc cgaccccgcg cctcctcggc
    10201 atcctccaga aggccggcac ggccgcggta cgcaccgaca cgggcggaat gttgctcgtc
    10261 ggccccgcac ccggcggtcg gctgagtgtc tggtccgaga cggcaccgta gccggcgcgc
    10321 gataacatct catccaagac gcttcgggag ggagggtatc gatggcctcc cggggaacct
    10381 cgcgccgatc ggcacgcgcc gcggaggtga aggcggcgat acctcgcctc tcgtgggcga
    10441 aggcccgccc ggccccggtc gtgctcgtct ccggccggga aaacgtgctc gccgaccggg
    10501 cgatcgcgca catcaaagag gccctcctgg caacggaccc gaacctcgaa gtcaacgaca
    10561 tcgacgccgc aggctacggc gcgggggaac ttctcaccct cgcgagccct tcgctcttcg
    10621 ccgacccgcg gctgatccgc gtcgaggccg tcgaggccgc aagcgacccg ttcctcaccg
    10681 acgccctcgc ctaccttgcg gcccccgccg acgacacggt gctcgtcttg cgccaccacg
    10741 gcggcacccg cggcaaacgt ctcctcgaca gggtgcgtgc cggccttggc ggcggcgtcg
    10801 aggtcgtctg cgccgaggtg acgagggatc gtgaccgggc cgacttcgcg cgcgccgagt
    10861 tcggccgcgc ggggcgcgag gcgacgcccg gcgcggcccg ggccctcgtg gacgcgttca
    10921 acgacctcgc cggcctggcc gccgcctgct cgcagctggt ctctgacacg gaaggcctcg
    10981 tcgacgaggg cgtcgtgcgc cggtactacg ccggccgcgt cgttgccaac ggcttcgccg
    11041 tcgccgacca ggcgctcgcc ggccgggcgg gtctcgccct cgtcacgctc cggcaggcgt
    11101 gggagtcagg caccgacccg gtgccgatcg tcgcggcact ggcaagcaag attcgtgcca
    11161 tggcgcgggt cgccgccctg cgcggcaccg acgctgccgt cgcgcgccag gccggcatgg
    11221 cgccgtggca ggtggaacgg gcgcggcgag acctgcgcgg ttggggcgac acttcgctcg
    11281 cccgggcaat cctcgccgtc gccgacgccg acgcggcggt caagggcgcg gcgcgcgacc
    11341 ccaagtatgc ggtcgaaaag gccgtgcgtc tgatcgcccg cgaggcccgc ggctagagcg
    11401 cggcgacctg agaggcgatt gccgacttgc ggttcgcggc ctggttcttg tgaatgacgc
    11461 ccttgctcgc ggccttgtcg agcttcttcg cggcgacgga aagcgcgctc gtcgccttct
    11521 ctttgtcacc cgcggcgacg gcctcgcgcg tcgcccggag cgcggtgcgc aactcgctct
    11581 tgacggcctt gttgcgttcc tgagccttgc ggttggtgcc gacacgtttg atctgcgact
    11641 tgatgtttgc cacgggggat agacgtcttt cgttcggaat tctgcgatac gtaccgtcgc
    11701 cggcacgaac caacgacgaa caggcgtgct ctcacacgca agccaaacac aaactttatc
    11761 aaagtgcgcc cgacggttcc acctgggtgg cgggcccgct ccgggcctcc tcacgctgca
    11821 ccgtagaatc gaggcaccat ggcaacacga gcggagagcg cgccccggcc cgccgcgacc
    11881 gacccggcgc tcatccgcaa cttctgcatc atcgcgcaca tcgaccacgg caaatccacg
    11941 ctggccgacc gcatgctgca gctgaccggt tccgtcagtg aacgtgacat gcgggcccaa
    12001 tacctcgacc gcatggacat cgagcgggag cggggcatca cgatcaagag ccaggcggtg
    12061 cggatgccgt ggcaggcggg cggccgcggc tacgtgctca acatgatcga cacccccggc
    12121 cacgtcgact tctcgtacga ggtgagccgt tccctcgccg cctgcgaggg ggccgtgctg
    12181 ctcgtcgacg cgtcgcaggg catcgaggcg cagaccctcg cgaacctgta tctcgcactc
    12241 gaccacgacc tcacgatcct gcccgtcttg aacaagatcg acctgccctc cgccgacccg
    12301 gaccgatacg cccgggagct ctccggcctg atcggcggag acccggccga cgtgatgcgg
    12361 gtctccgcga agaccggcga gggcgtcacg gcgctcctcg accgcctcgt ggaacgtgtg
    12421 ccgccgcccg cgggcgagcg gcaggaggcc agccgggcgc ttatcttcga ttcggtctac
    12481 gactcctacc gaggcgtcgt cacctacgta cgcatggtcg acggcgtgct gcacgctcgt
    12541 gaccgcatcc ggatgatgtc gacgggcgcc actcacgaga ttctcgagat cggcgtggcg
    12601 agccccgagc cgacgccgac gcaagggctg gccgtgggag aggtgggcta tctcatcacc
    12661 ggcgtgaaag acgtgcgcca gtcgaaggtg ggcgataccg tgaccgacgc gtcgaacccc
    12721 gccgccgagg cgctcgccgg ctacacggag cctctgccga tggtcttctc cggcctctac
    12781 ccgctcgacg gcagcgacta ccccgacctg cgggacgcgc tcgacaagct gaagctctcc
    12841 gacgcagcgc tcgcctacga gccggagacc tccgtggccc tcgggttcgg cttccgttgc
    12901 ggctttctcg gcctgctgca cctcgagatc gtgacggagc gtctgcagcg cgagttcggg
    12961 ctcgacctca tcgccaccgc gccgagcgtg acctaccacg tgacggccga agatggcacc
    13021 gaactgacgg tcacgaaccc gagcgaattc ccgccgggcc ggctcgtgtc cgtgcgggag
    13081 cccgtcgtgc gggccgcgat tcttgccccg aaggagtatg tcggcgtcat catggagctg
    13141 tgccagggca agcgcggcac cctcgaaggc cttgactacc tgagcgagaa ccgggtcgag
    13201 atcggctaca cgatgccgct cggcgagatc gtcttcgact tcttcgatca gttgaaatcg
    13261 aagaccgcgg gttacgcgtc tctcaactac gagccggcgg gcgaggcgga ggccgacctc
    13321 gtgaaggtcg acatcctctt gcagggcgag cgggtcgacg cgttcagcgc gatcgtgcac
    13381 cgcgacaagg cgtacgacta cggggttctc atgaccggcc ggctgaagaa gctcattccg
    13441 cgccaacagt tcgaggtgcc catccaggcg gcgatcggcg ctcgcatcat cgcccgcgag
    13501 acgatccgcg cgatgcgcaa agacgtgctc gccaagtgct acggcggcga catcacccgc
    13561 aaacgcaagc tgctcgagaa gcagaaggag ggcaagaagc gcatgaagac cctcggccgg
    13621 gtcgacgtgc cgcaagaggc gttcatcgcc gccctctcgg gcgacgtcga cacgagaaac
    13681 gaccagcgaa agaagtaagg gatgcccgcg ccactgccgc aaggcgagcc cgcgccagaa
    13741 gacggcctgc tgccgcccac cgcccgcgag ggcgccgccc ggcgggcctt cggggtctac
    13801 ctgcacgtac cgttctgccg cacccggtgc ggctactgcg atttcaacac ctataccgcg
    13861 acggagctgc agggcgccaa gcaagaggac tatgcggccg aggcggcccg cgagatccgc
    13921 ttcgcgggcc gtgcgttgcg ccgctccggg gtgccgggcc gcctcgccca gaccgtgttc
    13981 ttcggcggcg gcacgcccac cctgctgccc tccgatcatc tcgcccgcat gctcggcacg
    14041 gtgcgcgagg tatggggcat cgccccgggc gccgagatca cgacggagtc gaaccccgac
    14101 tcggtcgacg agcgctactt ggccgagctc gccgaggccg gcttcacacg tgtctcgttc
    14161 ggcatgcaat ccgccgtgcc gcacgtcttg aagacactgg agcgcacaca cgacccggcc
    14221 cggatcccgc tcgtcgtccg ttgggcgcgc cgcgccggcc tcgatgtcag cctcgacctc
    14281 atctacggca cgccgggcga gagcctcgac gactggcgtt gctccctcga tgccgcgctg
    14341 gagcaggagc ccgaccacat cagcgcctac agcctcatcg tcgagccggg cacgaagctc
    14401 gcccggcagg ttcggcgcgg tctcgtgccg gaacccgacg acgacctcgc ggccgacgag
    14461 tacgagctcg ccgacgaggt gctcggccgg gccggtttcg actggtacga gatcagcaac
    14521 tgggccaggc gggcgccggg ggagaccggg cgggcgatgg cgcaccgctc ccggcacaac
    14581 ctggcctact ggcgtgatgc cgactggtgg ggaatcgggc cgggcgccca cagtcatgtc
    14641 gggggcgtgc ggtggtggaa cgtgaagcat ccggccgcct acgcacaccg ggtgcaggcc
    14701 ggcctctccc cggctgccgg ccgtgaggtg ctcgacgagg cgacccgcac gatggaggcg
    14761 atcatggtag gggtgcgcat cgccgagggg ttcccagtgg cgcgcctggg cgaggcagcc
    14821 cgctccgagg tgcccggcct cgtggccgag ggcctcctcg agccggaaga ggccgcccgc
    14881 ggcatgctcg tgctcaccct gcggggtcgg ctcttgggtg acgcggtcgt ccgacgactc
    14941 acgtgacggg cgtgcccggg cctgcgtcgt cctggctgcc cggggaacgg gcacggtata
    15001 gttggcactc aaggtgtatg agtgctaagc atcggtcggc ctacagagcc ggttctgggc
    15061 gggaggcggc gatgatctcg gaacgcagcc tcgacgtcct gcgcgtcatc gtgcaagatt
    15121 atgtcgccaa caacgaaccc gtcggctcga agacgatcgt cgagcggcac gacttcggcg
    15181 tgtcggccgc gacgatccgc aacgacatgg cctctcttga agaagaggac ttgatcgtgg
    15241 ccccgcacac ctcgtcgggc cgggtgccga ccgacaaggg ttaccgcctg ttcgtcgatc
    15301 gcctggcgac cgtccggccg ctcagccccg cccagcgcct cgcgatcgaa cgcttcctcg
    15361 gtgaatcggt cgacgtcgac ggcgtgctgg agcgcaccgt gcggcttctc tcgcagctca
    15421 cccaccaggt cgcgctcgtg caatacccgt cggcggcgca ttccgccatt cgccgcattg
    15481 agttcgtctc gttgggcccc aacaaggtgc tgtgcgtgct catcgccgcg ggcggcgtcg
    15541 tcgagcagcg ggttgtcgac ctgccgcgtg acgcgggcga agactggctc ggcgtcgtgc
    15601 aagaccgggt gaacggcgcg gtgcacggtc tggaggtcgg cgacgccctc gcccgccttg
    15661 ccgagttggc ggaggcgacc ggcggctccg accgcgacgc gggtctcgcg ctcgtcgagg
    15721 cggtgcgtga gcagctcgcc gtggaacggc acgatcggct cgtcgtcgcc ggctcctcca
    15781 acctcatccg cggcgagcgt gacttcaacg gcaacatcag cccggttttg gatgccttgg
    15841 aggagcaggt cgtcttgctg cgtctcttca gcgagatgga ggaggatcgg cggggtgtca
    15901 gcgcgagcat cggccgggag aacgcgtcgt tcgggctcgg cgaggcgagc gtcttgagcg
    15961 ccagctatac cgggacgggc ggccgggcgg cgcacctggg cgtgctcggc ccgacccgca
    16021 tggactacac cgccaacatg gcggcggtgc gggccgtggc acgttacctt tccaggcttt
    16081 tcggcgagaa catctagtca gcgtgaggac aaggcacctg tggccgatca ttacgagatc
    16141 ctgggcgtcg agcgcgacgc caccccagac gagatcaaga aggcgtaccg caagctggcc
    16201 cgtcggctgc atcccgatgt gaacccgagc cccgacgcgg ccgaacgctt caagctcgtc
    16261 acccaggcgt acgacaccct gagcgacccg gaacgccggc gccggtacga catgggcggc
    16321 gaggaacaca acttcgccgg ccagggcttc ggcgggttca gcgacatctt cgagaccttc
    16381 ttcggcggcc agggcgcggg ccgcggcccg cgttcccgcc gagagaacgg gcaagacgcg
    16441 ctcctgcaca tcgacgtgga actggccgac gtgatcttcg gcacgcatcg cgacatcgag
    16501 gtggacacgg ccgtcgtatg cgagacgtgc ggcggctcgt gctgccagcc cggcacggcg
    16561 cccgtgacct gcgacatctg ccacggcacc gggcacgtgc agcgcacggt gcgctcgctg
    16621 ctcggcaacg tcgtcacctc cacgccgtgc ggcacctgcc gcggctacgg cacgatcatc
    16681 gcgaccccgt gcagcacctg ccaggggcgg ggccgggttc gggcccacaa gacgatcccg
    16741 gtcgacatcc cgtcgggggt ggagacgggg ctgcgcctgc agatgccggg cagcggcgag
    16801 gcgggccctg gcggcggcgc gaacggcgat ctgtatttgg agatcacggt gcgtaaccac
    16861 gacgtcttct cccgagacgg cgacgatctg ttgtgcacgc tcgacgtcga gatgtccgac
    16921 gcgattctcg gcaccaagac cacccttcag accctcgacg gcgacgtcga cgtggaggtg
    16981 cggcccggca cccagagctc cgaggtcatc acggtccagg gccgcggcat aacgcatctg
    17041 cgcggcagcg gccgcggcga cctgcgcatc ggcgtgcacg tcgtcacccc gacgaagctc
    17101 gaccacaagg accgcgacct catcaagcgc ttcgccgagg gccgcaagca gcagccgccg
    17161 cgcctcgccc gcgccggcca aggagtgttc cagaggctgc gcgaccggtt catgcgggtc
    17221 tgaggggccg tggcgtctct cttctacgac gagagcctgg ccgatacggg cgaaggcttg
    17281 cccgaggtcg gtgccgtgtg ccggctgagc ggcgcggagg cgcatcacgc cgccgtgaac
    17341 aggctgcgcc cgggcgagac cgtgtcggtc ggcgacggcg cgggcacgat cgtcacgggc
    17401 ccggtcgtga cgctcgatcg agaaggcctg gcggttcgcg tcgagcgccg ggtgcgaaag
    17461 ccgcggccgg ccccgcggct cggcctcgcc caggcgctcg ccaagggggg ccgtgacgag
    17521 ctggccgtgc aggccgcgac cgagctcggc gtcgacgtcg tcgttccgtg gatggcgagg
    17581 cgcagcgtgg cccgctggtc gggtcagaaa cgcgaccgcg gccccgaacg ttggcgtgcg
    17641 atcgtgcgcg aggcgggcaa gcagtcgttg cgcgcgtggc tccccgaggt tctgccgccg
    17701 cattccacgg cggagctggc cggccttgcg ggcgtctggc agctcgccgt gctcgatccc
    17761 gcttcaaacc tcacgatgac cgcctggctc ggcacaggac cgcgcgccca gggcggcggg
    17821 ctgtcggccg ccgggggcgg ggcggcgggc cccgatcgcc gcgacgtact gctcgtcgtc
    17881 gggccggagg gcggcatcgc ggccgacgag gcgcggcttt tggaggaggc gggggcggcg
    17941 cgcctccggc tcggccagac ggtgctgcgc acctcgacgg ccggccccgc ggcgatcgcc
    18001 gtctggaacg cgtgcggggg ccggcgctgg tgaggcccgc acccgcggcc cgtaggatgg
    18061 tagccatgcc cggtgacgcc gactccgtgt tcacgaagat catcgcgcgg cagatccctg
    18121 ccgacatcgt ctatgaagat
//
LOCUS       DASTNL010000166         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1593788, whole genome shotgun sequence.
ACCESSION   DASTNL010000166 DASTNL010000000
VERSION     DASTNL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593788"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1055)
                     /locus_tag="VFQ96_05195"
     CDS             complement(138..1055)
                     /locus_tag="VFQ96_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEU0257219.1"
                     /translation="MSTAPGRIRIDIAYDGGRFHGWAAQPGLRTVQGTLEEAVARLLR
                     VPQAAAPRLTVAGRTDAGVHATGQVAHLDLTGEEWGALAGSHAGRARTLAARSAPEPP
                     PGPDAVDIACRRINGALRADDVRVLGGRIAPDGFDARFSALWRRYLYRVADGAGAHDP
                     LERHRTLWHPTRLDVSLMDRAARQLGGLHDFAAYCKPRPGSTTIRELQDFRWRRAPDG
                     VLEARVRADAFCHGMVRALVGACIAVGCGRLEPHDPLGLRNAGRRTSAFAVAPAHGLT
                     LVEVGYPDEDELAERARQTRALRTLTRPD"
     gene            1206..>2102
                     /locus_tag="VFQ96_05200"
     CDS             1206..>2102
                     /locus_tag="VFQ96_05200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU0257220.1"
                     /translation="MTSFRNVLPSAPAPAPPAPPQHLAGLDGLRGIAVLAVIAYHLFP
                     SVAPGGFLGVDVFFVISGFLITHLLILEHERLGRLRLARFWIRRARRLLPALAATVLV
                     CGAVAAMIGGDTLVGIGRQVLGATTFSSNWIAAFTGQSYFSASTPDLFRNFWSLAVEE
                     QFYLIWPLALLLLLRLRPSGARVAVMAVLALSSATEMALLFRAGRDASRVYYGTDTHS
                     FGLAVGAALALWLAGARAAGRGGQTRPPSAATRWLPAAGLACLAAVVTAVFVLPDDRA
                     VTYRGGILVVSMAAAVVIWASGT"
BASE COUNT          242 a          798 c          735 g          327 t
ORIGIN      
        1 gaggtggtcg tgattcccgc tccgtggatg ttcggcacgc gatgcggccg aagcctccgg
       61 tggagggctc acgttcgatc cccgcggaaa ccgcgcgggt accaagcgat aactctatcg
      121 aagagccgcc gcgctcgtca atccggcctc gtcagggtgc gcagggcccg ggtctgccgg
      181 gcacgctcgg cgagctcgtc ttcgtccgga tagccgactt cgacgagggt cagcccgtgg
      241 gccggcgcga cggcgaaggc gctcgtgcgc cggccggcgt ttcgcaaccc gagcgggtcg
      301 tggggctcca gccggccgca cccgaccgcg atgcaggcgc cgaccagtgc gcgcaccatg
      361 ccgtggcaga acgcgtcggc ccgcacccgc gcctccagca ccccgtcggg ggcacggcgc
      421 caccgaaagt cttgcagctc gcggatggtc gtcgagcccg gccgcggctt gcagtatgcg
      481 gcgaaatcgt gcaggccgcc gagctgcctg gccgcgcgat ccatgaggct cacgtcgagc
      541 cgcgtcggat gccagagggt gcgatggcgt tccagggggt cgtgggcgcc cgcgccgtcg
      601 gccacgcggt acaggtagcg ccgccacagc gccgagaaac gcgcgtcgaa cccgtcgggc
      661 gcgattcggc cgccgaggac ccgcacgtcg tcggcgcgca gcgcgccgtt gatccttcga
      721 cacgcgatgt cgacggcgtc ggggccgggg ggaggctccg gggcagaccg ggcagccagc
      781 gtgcgcgcgc ggccggcgtg gctgcccgcc aacgcgcccc actcctcgcc cgtcaggtcg
      841 aggtgggcga cctgaccggt cgcgtgcacc ccggcgtcgg tgcgccccgc gaccgtcagg
      901 cgcggggccg ccgcctgcgg cacccggagg agccgggcca cggcctcttc gagcgtgccc
      961 tgcacggtgc gaaggcccgg ctgcgccgcc cagccgtgaa accggccgcc gtcgtacgcg
     1021 atatcgatcc ggatccggcc aggggcggtg ctcacggcaa accatcctac gatcgcctcc
     1081 cgggccgggt cgggccgcct ggcccgcacg acacgcctgc cggcggggcc tcttgtcagg
     1141 ctgcgctgtt atcgtgggcg cgcctgcctc gtccaccctc ttctcacggt cgtatcgcct
     1201 tttccatgac ctcgttccgc aacgtccttc cgtcggcgcc cgcgccggcg ccgcccgcgc
     1261 ctccgcagca cctggccggc ctcgacgggc tgcgcggcat cgcggttctc gccgtgatcg
     1321 cctaccacct gttcccctcg gtcgcgccgg gcgggtttct cggcgtcgac gtgttcttcg
     1381 tgatcagcgg cttcctcatc acgcatctct tgatcctcga gcacgaacgg ctgggccggc
     1441 taaggctcgc ccggttctgg atccggcgtg cccggcggct gctgccggcg ttggccgcca
     1501 cggtgctcgt ctgcggcgcc gtcgcggcca tgatcggcgg ggacaccctc gtcggcatcg
     1561 gcaggcaggt cctcggcgcg acgacgttca gcagcaactg gatcgccgcc ttcacggggc
     1621 agagctattt ctcggcaagc actcccgacc tgttccgcaa cttctggtca ctcgccgtgg
     1681 aggaacagtt ctatctcatc tggcctcttg cgttgctcct gctcttgagg ttgcgcccca
     1741 gcggcgcccg cgtggctgtg atggccgtcc tcgccctctc gtcggccacg gagatggcgc
     1801 tcctcttccg cgccggccga gacgccagcc gcgtctacta cggcaccgac acgcacagct
     1861 tcgggctcgc ggtcggcgcg gccctcgcat tgtggctggc cggggcccgc gcggcaggcc
     1921 ggggcggcca gacccggccg ccttccgccg ccacccgctg gctgcccgcg gccggtctcg
     1981 cctgcctcgc cgcagtcgtc accgcagtgt tcgttctgcc cgacgaccgg gcggtgacgt
     2041 accgcggcgg gatcctcgtc gtcagcatgg ccgcagcggt cgtcatctgg gcctccggca
     2101 cg
//
LOCUS       DASTNL010000167         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_670748, whole genome shotgun sequence.
ACCESSION   DASTNL010000167 DASTNL010000000
VERSION     DASTNL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670748"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFQ96_05205"
     CDS             <1..577
                     /locus_tag="VFQ96_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU0257221.1"
                     /translation="RRLGIRPGQQALLYAPTFRDYVSADDTRAEMIDFVDLGRLARRL
                     GDDYVILLRGHPFNARSGTQAEGRVVNVTDYPDINDLIVASDLAVLDYSSLRFDYALT
                     GKPAIFFVPDRERYFRGREGFLPYDETTPGPQITAAADLERAILDAPRLAAEHEEARR
                     AFLSRFMELEDGHATQRLIDRVFGSVRGRDC"
     gene            complement(558..2414)
                     /locus_tag="VFQ96_05210"
     CDS             complement(558..2414)
                     /locus_tag="VFQ96_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEU0257222.1"
                     /translation="MRRHTKMTHATTHDTMTEPTTDEDQAAGHRGARLAEAGRRLVGR
                     PARRALSLVAAGALAAALLVAGQVATNPAPAQALSGSSFNPGNIISDQVFSNSGSMTA
                     SQVQSFLNTQGASCTTTSSYTCLKDYHQTTTALPDANQHCSPYQGASNESAATIIVKV
                     AKACDINPQVILVTLQKEEGLVTMANSAAKPSGAAGRYQIAMGFGCPDSAPCNTQYYG
                     FFNQVFQAARQFDVYRTNQGLYGFRAAGQTYNVLYSPTSSCGSAPVTMANWATAGLYD
                     YTPYQPNAAALANLYGAGNSCSSYGNRNFWVYFNQWFGSTQVQETNVNMTVTNSSGQP
                     MSGVAVAFYKASASTTTVSKPALTTTTSSTGTASALLQKGSYKAAFVASDGTTISGAP
                     GISDLPYMTTWAGGTTSAAGAKVITAGSSASTALPVTVATWQAPTNSPFKDVTSSTQF
                     YSQIAWLSTAGISEGYANGTYQPGTTVKRNAMAAFLYRMAGSPAYTPPKTSPFKDVST
                     TNQFYQQIAWLASQGISTGYADGDFKPDATVDRNAMAAFLYRFAGSPTFTAPTQSSFK
                     DVTPTTQFYDEISWMDSTGISQGYADGDYKPDASIKRNAMAAFLYRFNSLGL"
BASE COUNT          390 a          920 c          892 g          474 t
ORIGIN      
        1 gcggcggctg gggatccggc ccggccagca ggccctgctc tacgcgccca cgttccgcga
       61 ctacgtctcc gccgacgaca cccgcgccga gatgatcgat ttcgtcgacc tcggcaggct
      121 cgcccgccgg ctgggagacg actacgtgat cctgctgcgc ggccacccgt tcaacgcccg
      181 cagcggcacg caggcggagg gccgggtcgt gaacgtgacc gactacccgg acatcaacga
      241 cctcatcgtc gcgagcgacc tggccgtgct cgactactcg tcgctgcggt tcgactacgc
      301 cttgaccggc aagcccgcga tcttcttcgt gcccgaccgg gagcgctact tccgcggccg
      361 ggaggggttc ctgccctacg acgagacgac gcccggtccg cagatcacgg ctgcggccga
      421 cctggagcgc gccatcctcg acgcgccgcg cctcgccgcc gaacatgagg aagcccggcg
      481 ggcgttcctc tcccgcttca tggagctgga ggacggccac gccacccagc gcctcatcga
      541 ccgcgtcttc ggctctgtca gaggccgaga ctgttgaagc ggtagaggaa tgccgccatc
      601 gcattgcgtt tgatcgaggc gtccggcttg tagtcgccgt ccgcatagcc ctgggagatg
      661 ccggtcgaat ccatccagct gatctcgtcg tagaactgcg tcgtgggcgt cacgtccttg
      721 aacgaggact gcgtgggggc cgtgaaggtc ggcgagccgg cgaagcggta gaggaacgcc
      781 gccatcgcat tgcgatccac cgtcgcgtcc ggtttgaagt cgccgtccgc atagccggtg
      841 ctgatgccct gagaggccag ccaggcgatc tgttgataga actggttcgt cgtggaaacg
      901 tccttgaacg gcgacgtctt cggcggcgtg taggcgggcg agccggccat ccggtagagg
      961 aacgccgcca tggcgttgcg tttcaccgtc gtgcccggct ggtaggtgcc gttcgcgtac
     1021 ccctcggaga tgccggctgt ggagagccac gcgatctgcg agtagaactg cgtcgagctc
     1081 gtcacgtcct tgaacggcga attcgtgggc gcctgccagg tcgccacggt caccggcagc
     1141 gccgtggagg cgctggagcc ggcagtgatg accttggccc cggccgccga cgtcgtgcct
     1201 cccgcccagg tcgtcatata gggcaggtcg gagattccgg gcgcgccgga gatcgtcgtg
     1261 ccatcggagg cgacgaacgc cgccttgtag ctgcccttct gcagaagcgc gctggcggtg
     1321 ccggtcgagc tggtcgtcgt ggtcagggcc ggcttcgaga cggtcgtcgt cgaggcgctg
     1381 gccttgtaga aggcgaccgc gacacccgac atgggctggc ccgacgagtt cgtgaccgtc
     1441 atgttcacgt tcgtctcctg cacctgcgtg gagccgaacc actggttgaa gtacacccag
     1501 aagttccggt tcccgtagga ggagcagctg ttgccggcgc cgtagaggtt cgccagcgcc
     1561 gccgcgttcg gttgatacgg cgtgtagtcg tagagcccgg ccgtcgccca gttcgccatc
     1621 gtcaccgggg cggagccgca ggacgaggtc gggctgtaga gaacgttgta ggtctggccg
     1681 gcggcacgga agccatagag gccctggttg gtgcgataca cgtcgaactg ccgcgccgcc
     1741 tggaacacct gattgaagaa gccgtagtac tgggtgttgc agggtgccga gtcggggcag
     1801 ccgaagccca tcgcgatctg atagcgcccc gcggcaccgc tcggcttggc ggcgctgttc
     1861 gccatcgtca ccaggccctc ctccttctgg agggtgacga ggatcacctg cgggttgatg
     1921 tcgcacgcct tggcgacctt gacgatgatc gtcgccgcac tctcattgga ggcgccctga
     1981 tacggcgagc aatgctggtt cgcgtcgggc agtgcggtcg tcgtctggtg gtagtccttc
     2041 aaacaggtgt agctggaggt cgtcgtgcaa ctggctccct gcgtgttgag gaacgattgc
     2101 acctggctgg cggtcatcga cccgctgttg ctgaagacct gatcgctgat gatgttgccc
     2161 ggattgaagc tgcttccgga gagcgcttgc gccggcgccg gattcgtcgc cacctgcccg
     2221 gcgaccagaa gagccgcggc cagtgccccc gccgccacga gcgacagtgc ccgccgtgcc
     2281 gggcgtccca ccagccgacg tcccgcctcg gcgagcctcg cccccctgtg tcctgccgcc
     2341 tggtcttcgt ccgtcgtagg ttctgtcatc gtgtcgtgtg tcgtcgcatg cgtcatcttc
     2401 gtgtgtcgtc tcaccgcgtg catactgccc ccgcgctcct tgcctgccct gcctgtgccg
     2461 cacccgaacc ggtcaccgcc gcgatccgtg caccgacgcg ccgcctgccg gcacgcctgc
     2521 gcccgatcga ccgctcccgc cgcaccgtcg ggtggactcc accgtccgtg acccctatcg
     2581 gccgcagcgc tcccgtcgca aggcacatga tggcctatcg tacgcggcac cccccagaca
     2641 cccaacgccg acacggcccc gcgtcgccgc gcaccc
//
LOCUS       DASTNL010000168         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_300950, whole genome shotgun sequence.
ACCESSION   DASTNL010000168 DASTNL010000000
VERSION     DASTNL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300950"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFQ96_05215"
     CDS             complement(<1..509)
                     /locus_tag="VFQ96_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0257223.1"
                     /translation="MSALTPATGMISVAEFVGRGLVKLGATRCFGVVGSGNFEVTNAV
                     KRAGGTFVAARHENGAAMMADGFARMSGEVALLTVHQGCGLTNAGTGICEAAKSRTPL
                     IILAAESPAFQVNSNFAMDQAGFARSVGAAAERIHGQASAARDLVRAWRTARNQHRTV
                     VLNLPLDVQA"
     gene            complement(509..1294)
                     /locus_tag="VFQ96_05220"
     CDS             complement(509..1294)
                     /locus_tag="VFQ96_05220"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0257224.1"
                     /translation="MSVVESLIASLAGEGGVRVVDLTTPLSAETPTLRLPDPFVNLID
                     FSLEEVSAYNAPGPFWKHHNIHTGEHIGTHVDAPVHWITGRDGRDVSQIPPERLVGPA
                     VVIDKTAEVRANPDYLLTADDIRAWEAEHGAIASGSWLLVHTGWAQYGADRERFLNAN
                     ESGPHSPGIEPATARWIAEKLPISGLGVETVGIDAGQAGGFDPAFPAHNALLGHDKYG
                     LTSLRNLDLLPARGALVVVAPLPIVGGTGSPARVFALVDERAA"
     gene            complement(1454..1852)
                     /locus_tag="VFQ96_05225"
     CDS             complement(1454..1852)
                     /locus_tag="VFQ96_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEU0257225.1"
                     /translation="MSEAAIPGVELAERMGIQVIELSADHAVATMPVEGNRQVSGVVH
                     GGAYCVLAETLGSLAAGVHAGPGWVALGIDINATHTRAASSGIVTGECTALFLGRTMT
                     VHEIVVTDEQGRRLSTARITNTLRRLPADA"
BASE COUNT          345 a          751 c          778 g          325 t
ORIGIN      
        1 gcctgcacgt cgagcggcag gttcaggacg actgtgcggt gctggttgcg tgcggtgcgc
       61 caggcgcgta cgaggtctcg ggcggcgctc gcctggccgt ggatgcgctc cgcggctgcg
      121 ccgaccgacc gcgcgaagcc tgcctgatcc atcgcgaagt tcgagttcac ctggaaggcg
      181 ggggattcgg cggccaggat gatgagcggc gtacgactct tggccgcctc gcagatgccg
      241 gtgccggcgt tggtgagccc gcaaccctgg tgcacggtga gcagcgcgac ctcgccgctc
      301 atgcgtgcga aaccgtcggc catcatcgcg gcaccgttct cgtgccgggc cgcgacgaac
      361 gtgccgcccg cacgcttcac ggcgttcgtg acctcgaagt tgcccgagcc gaccacgccg
      421 aagcatcgcg tggcgcccag cttcacgagc ccgcggccca cgaactccgc gaccgagatc
      481 atgcccgtcg cgggggtgag ggcagacatc aagcagcccg ctcgtcaaca agcgcgaaca
      541 cgcgggcggg gctgccggtg ccgccgacga tgggcagggg ggcgacgacc acaagcgctc
      601 cccgagcggg gaggaggtcg aggttacgca gcgaggtgag cccgtatttg tcgtggccca
      661 agagggcgtt gtgcgcgggg aacgccgggt cgaaaccgcc ggcctggccg gcgtcgatgc
      721 cgaccgtctc cacgccgagg cccgagatcg gcagtttttc ggcgatccat cgggcagtcg
      781 cgggctcgat gcccggagaa tgcgggccag actcgttcgc gttcaagaaa cgctcccggt
      841 cggcgccata ctgcgcccat ccggtatgca ccaagagcca cgagcccgac gcgatcgcgc
      901 cgtgctcggc ctcccaggca cggatgtcgt cggccgtcag caggtagtcc gggttcgccc
      961 gcacctcggc cgtcttgtcg atgacgacgg ccgggccgac gagccgctcg ggcgggatct
     1021 gcgaaacgtc acggccgtca cggcccgtga tccaatgcac gggggcgtcg acgtgggtgc
     1081 cgatgtgctc gccggtgtgg atgttgtgat gcttccagaa cgggcccgga gcgttatacg
     1141 cgctcacctc ttcgaggctg aaatcgatga ggttcacgaa cgggtcgggc aggcgcagag
     1201 tcggcgtctc ggccgagaga ggcgtcgtca ggtcgacgac gcgcacaccg ccttctcccg
     1261 ccagagacgc gatgagactt tccacaacgg acacggtcac tccttcttct ttgacgggta
     1321 gcgcggcgct cgggtaatcc accctccgcg ccgaaggggc cggatacggg gatccggccg
     1381 accgtcacca tgatgcaacg gcccccaccg cccggctgtc ccaggtgcgt atcctgcggc
     1441 caagcactcc ggctcacgca tccgccggca gtcgccgcag cgtgttggtg atgcgggccg
     1501 tcgagaggcg tcggccttgc tcgtcggtca cgacgatctc gtgcaccgtc atcgtgcgcc
     1561 ccaagaacaa cgcggtgcat tcgccggtga cgatgcccga tgaggccgcc cgcgtgtggg
     1621 tcgcgttgat gtcgatgccc agggcgaccc agccgggccc ggcgtgaacg cccgcggcga
     1681 gtgagccgag cgtttcggcg agcacgcagt acgcaccgcc gtgcacgaca ccgctaacct
     1741 gacggttgcc ttccacgggc atcgtcgcca cggcgtggtc tgccgacagc tcgatgacct
     1801 ggatgcccat ccgttcggcg agttcgacgc ccggtatcgc cgcctcactc atggagtgct
     1861 ctcacgacac gaatgagcgc ctgctccccg ggcacggccc cgggccgcca gaggcaggtg
     1921 acgacccaag gcctgccggg accggggaag gacgaggcag tgcggtggtt cacgacggtt
     1981 ctccttcatg gtcgctgtgg ccgacgttgt tgtcacgact ctacggccct ggggcgacga
     2041 cacggcggca ccgccacgaa gcacgggcgt cggcgacaac cgacgcacac ctgccgggcg
     2101 gcgcgggaac acacccaggg tggaacctgg gcggcggccg gcagccggca ggggaggaac
     2161 cctgtcaggc ggcgctcttc agcacggcgg ccgtgaagc
//
LOCUS       DASTNL010000169         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1598307, whole genome shotgun sequence.
ACCESSION   DASTNL010000169 DASTNL010000000
VERSION     DASTNL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598307"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFQ96_05230"
     CDS             <1..861
                     /locus_tag="VFQ96_05230"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU0257226.1"
                     /translation="EALLFAQDKEAMRRRLGESGFPVPAWAPVASADDLERFLGAHGG
                     VAVLKTARGGYDGHGVRVVTSGHDADDWLAALAQDDSTGSLLVEEKVDFRRELSQQVA
                     RRPSGGVAVYPVVETIQQDGVCAQVIAPAPETSDPVARECARIGRGVAEQLGVTGMLA
                     VELFETTDGRVLVNELAMRPHNSGHWSIDGAVTGQFEQHLRAVLDLPLGDPAPRAPWT
                     VMVNVLGGPAGRSLPSRYPEVFARHPRVKMHGYDKTSRPGRKVGHVTAYGDSLDTVLA
                     EARAAAALFS"
     gene            1075..1320
                     /locus_tag="VFQ96_05235"
     CDS             1075..1320
                     /locus_tag="VFQ96_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU0257227.1"
                     /translation="MKTAISLSDHDFERFERVASQHGMNRSEFYRAAARRLADELEGA
                     SELTAAANAALKSAGQPSGDGLFLRESERLLRENVEW"
     gene            1317..1661
                     /locus_tag="VFQ96_05240"
     CDS             1317..1661
                     /locus_tag="VFQ96_05240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEU0257228.1"
                     /translation="MIRRGDIVWVDFGRPRGSEPAKVRPAVVLQEEWLLATNIETVLV
                     VPLTSNTRLAEFPGNVLIPAEVSGLAKHSVAVVSQVGPVRREFLEPYPAGRAPAHVLT
                     QVAAGVRLVLGL"
     gene            1685..2263
                     /gene="purE"
                     /locus_tag="VFQ96_05245"
     CDS             1685..2263
                     /gene="purE"
                     /locus_tag="VFQ96_05245"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU0257229.1"
                     /translation="MPEPVAPEPAVATASAEPAAGAVPTFVNAASPRVAVVMGSDSDW
                     KVMSAASDVLREFRVPHQVEVISAHRTPEKMLRFGREAAGRGIRVVIAGAGGAAHLPG
                     MLAAVTTLPVIGVPVPLAKLDGLDSLLSIVQMPSGVPVATVSIGGAKNAGLLAVKILA
                     TADAELATALADYSAALAASVEAKNTALLSRL"
     gene            complement(2451..3488)
                     /locus_tag="VFQ96_05250"
     CDS             complement(2451..3488)
                     /locus_tag="VFQ96_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEU0257230.1"
                     /translation="MSDAPTTSVFEAQVARVRQISDVFTRVTLAGEQLAGFHPCGPLG
                     PRDLRVKLIFPEPGGRIRVRPEATRGWFPRWRALDPAERGLLRTYTARAIRHGPGATP
                     EVDIDFVMHGHDDESRGPGATWAAGVAEGDDVVLIGPSRAAPHPEGIEWRPPDASAAH
                     PVQVLLTADETAVPAVASILQTLPAGYRGHAILEVPSEAEIQPMPTDSSVETVWLTRG
                     GAPRGGALFDAVRSLPGWSPQAESRQSLPEAAAAPRTEGLMGHLFSRSRKHRPDPARR
                     QALPEVDIDKERLWEVAAPDSESRRRYAWIAGEAATVRRLRSHLVHACGIDRHDMAFM
                     GYWRQGMAEPN"
     gene            complement(3485..4453)
                     /locus_tag="VFQ96_05255"
     CDS             complement(3485..4453)
                     /locus_tag="VFQ96_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257231.1"
                     /translation="MTKATEKPATAAPTSRARPAGAAHATLEARQVRLGYGGEPIIDG
                     LDLAVIPGAITAIVGANASGKSTLLRGLDRLLTPQGGTVLLDGANIAGLPPRRVARRV
                     AILPQAPTAPDGITVGDLVARGRFPHQGWFRRRSADDERAVAAALAATTTQDLAWRPM
                     DALSGGQRQRVWLAMALAQETDILLLDEPTTYLDIAHQVELLDLVATLNEERGCTVVM
                     VLHDLNQAARYADHLVAMRGGQIVAAGPPGEIVTEQMVRRVFGMECRVLRDPVEGTPV
                     IIPAGRRRRARVAHDTPTARDTPATHSTPAGEKLPADEKALAKENG"
     gene            complement(4535..>4863)
                     /locus_tag="VFQ96_05260"
     CDS             complement(4535..>4863)
                     /locus_tag="VFQ96_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU0257232.1"
                     /translation="DSAAGLGLDVTRTKLLVLVVGVLLSAAATAVAGPVAFVAFLAGA
                     IARRAGRGRAPVTTAALVGVLIMLGAQLVSASLPDNVALPVGVVTGMLGAPFLLILVV
                     ATHRRG"
BASE COUNT          640 a         1682 c         1786 g          755 t
ORIGIN      
        1 gaggcgctcc tcttcgcgca agacaaagag gcgatgcgcc gtcggctggg tgaatcgggc
       61 ttccccgtgc cggcgtgggc accggtcgcc tccgctgacg acctggagcg gttcctcggc
      121 gcgcacggcg gagtcgccgt actgaagacc gcgcgtggcg ggtatgacgg ccatggcgtg
      181 cgcgtcgtga caagcgggca cgacgccgac gactggcttg cggccctggc gcaggatgat
      241 tcaaccggct cgttgctcgt cgaagagaag gtcgactttc gccgggagct ctcgcagcag
      301 gtggcacgcc gcccctccgg cggggtcgcc gtctacccgg tcgtcgagac gatccagcaa
      361 gacggtgtgt gtgcgcaggt gatcgcgccg gcgcccgaga cgagcgaccc ggtcgcccgg
      421 gaatgcgccc gcatcgggcg gggcgtcgcc gaacagctgg gtgtgaccgg catgctcgcc
      481 gtggagctct tcgagacgac cgacggccga gtgctcgtca acgagttggc gatgcggccg
      541 cacaacagcg ggcattggag catcgacggg gcggtgaccg gtcagttcga gcagcatctg
      601 cgggccgtgc tcgatctgcc gctgggagac ccggccccgc gcgcgccgtg gacggtcatg
      661 gtgaacgtgc tcggcggccc cgccgggcgc tcgctgccga gccgctaccc ggaggtgttc
      721 gcccgtcatc cgcgggtgaa gatgcacggc tacgacaaga cctcccgccc cggccgcaag
      781 gtcgggcacg tgaccgccta tggtgacagc ctcgataccg tgctcgccga ggcccgcgcc
      841 gccgccgcgc tcttctcctg agcgaggccg ccacgggggc caggccgctc tcgagccggg
      901 cccagccggt ttcccggatt ccgccggccg cgtcgccggc tcactcggcg cctcgtcgcg
      961 ggtcgcggcg accgaacgtg ttggagcctg tcgtggcggg agcagatggt gcgactcggt
     1021 atcgtcgctc ggatcgaagg aacccgtgtt ggtataaaag gttatacttc ggggatgaag
     1081 accgcaattt cactttccga ccatgatttc gagcgtttcg aacgggtcgc gagccagcac
     1141 ggcatgaacc ggtcggaatt ctatcgggcg gcggcgaggc ggctcgcgga cgagctggag
     1201 ggtgcctccg agctcaccgc ggccgcgaat gcggcgctga agagtgccgg gcagccctcc
     1261 ggtgatgggc tgttcttgcg cgagtcggag cgcctcctgc gcgaaaacgt cgagtggtga
     1321 ttaggcgggg cgacatcgtc tgggtcgatt tcggtcgtcc gcgtggctcg gagcctgcca
     1381 aggttcggcc cgccgtcgtg ctgcaagagg agtggctgtt ggcgacgaat atcgagaccg
     1441 tgttggtcgt gccgctcacg tcgaataccc ggcttgcgga gtttccgggt aacgtcctca
     1501 ttcccgcaga ggtgtcgggg ctcgcgaagc attccgttgc cgtggtctct caggtagggc
     1561 ccgtgcggcg agaatttctt gagccttacc cggccggacg cgcgccggct cacgtgctga
     1621 cgcaggtggc cgcgggcgtc cgcctcgtgc tcggcttgtg agcgccgccc atagcatggt
     1681 gggtatgcct gaacctgtcg caccggagcc cgccgtggcc accgcctctg ccgagcctgc
     1741 cgcgggggcg gtgcccacgt tcgtgaatgc ggccagcccg cgggtcgccg tggtgatggg
     1801 cagcgattcg gactggaagg tgatgtccgc cgcctccgac gtgctccgcg agtttcgggt
     1861 gccgcaccag gtcgaggtga tctcggcgca ccgcacgccg gagaagatgc tccgcttcgg
     1921 ccgggaggcg gcgggccggg gcatccgcgt cgtcatcgcc ggcgcgggag gcgccgcgca
     1981 tctgcccggc atgctcgccg cggtcaccac cctgcccgtc atcggcgtgc cggtgccgct
     2041 cgcgaagctc gacgggctgg actccctgct ctcgatcgtg cagatgcctt ccggcgtgcc
     2101 ggtcgcgacc gtgtcgatcg gcggcgcgaa gaacgccggg cttctggccg tgaagatcct
     2161 tgccactgca gacgccgagc ttgcaaccgc gctcgcggac tactccgctg cgctcgccgc
     2221 gagcgtcgag gcgaaaaaca cggcgctcct ctcccgcctc tgagccttcc cggcaccgct
     2281 gagcattcca gctgctgaga agcgctgagc agtcgatttg ctgaggcacc gattcgctgg
     2341 gccgctgggc cgtcggagcg tcgagtcact gaggcgccga gttccggagt cgttgagtcg
     2401 ttgagatgct gcgtcagtga gccgccctga ggccggccgg cgggggatcg tcagttcggc
     2461 tcggccatgc cttgtcgcca atagcccatg aatgccatgt cgtgccggtc gatgccgcag
     2521 gcatgcacga ggtgggaacg cagccgtcgc accgtcgccg cctcgcccgc gatccaggcg
     2581 tagcggcggc gggactccga atcgggcgcc gcgacctccc acagccgctc cttgtcgatg
     2641 tccacttcgg ggagggcttg tcggcgcgcc gggtcaggcc ggtgcttgcg gctgcgcgag
     2701 aaaagatgcc ccataaggcc ctctgttcgg ggtgcggccg cggcctcggg aaggctttgt
     2761 cggctctccg cctggggaga ccaacctggc aggctgcgga cggcgtcgaa aagcgcgccg
     2821 ccgcgcgggg cgccgccgcg cgtcaaccag acggtctcga cgctcgagtc ggtcggcatc
     2881 ggctgaatct cggcctcaga cggcacctcg aggatcgcgt ggccccggta tccggccggc
     2941 agggtctgaa ggatcgaggc gactgccggc acggccgtct cgtcggcggt gaggagcacc
     3001 tgcaccgggt gcgccgcgct agcatcgggc ggccgccatt cgatcccctc ggggtgtggg
     3061 gcggcgcggc tcggcccgat gagcaccacg tcgtcgccct cggccacgcc cgctgcccac
     3121 gtcgcgcccg ggccgcgtga ttcgtcgtcg tggccgtgca tgacgaagtc gatatcgacc
     3181 tcgggcgtgg cgcccgggcc gtgccggatc gcgcgggccg tataggtgcg tagcaacccg
     3241 cgctccgccg ggtcgagggc gcgccagcgc gggaaccagc cgcgcgtcgc ctcgggccgc
     3301 acccggatgc ggccgccggg ctcggggaag atgagcttca ccctgagatc gcgcggcccg
     3361 aggggcccac aggggtggaa tccggcgagc tgctcgccgg ccaacgtcac gcgggtgaag
     3421 acgtcggaga tctggcggac acgggccacc tgtgcctcga agacgctcgt cgtgggtgca
     3481 tcgctcatcc gttctccttc gccaaggcct tctcatcggc cgggagcttc tctcccgccg
     3541 gggtgctgtg tgtcgccggg gtgtcgcggg ccgtcggggt gtcgtgcgcc acccgggccc
     3601 tgcggcgccg accggcgggg atgatgacgg gcgtgccttc caccgggtcg cgcagcacgc
     3661 ggcattccat gccgaacacg cgacgcacca tctgctcggt gacgatctcg cccggcggcc
     3721 ccgcggcgac gatctggccg ccccgcatcg cgacgaggtg gtcggcgtag cgtgccgcct
     3781 ggttcaggtc gtgcagcacc atcacgacgg tgcagcctcg ctcttcgttg agggtggcga
     3841 cgaggtcgag aagctcgacc tggtgggcga tgtcgaggta ggtcgtcggc tcgtcgagca
     3901 gcaggatgtc ggtctcctgc gccaatgcca tggccagcca gacgcgctgc cgctgcccgc
     3961 cggacagcgc gtccatcggc cgccaggcga ggtcctgcgt cgtcgtcgcg gcgagggcgg
     4021 ccgcgactgc ccgctcgtcg tcggcgctgc ggcggcggaa ccagccctgg tgggggaagc
     4081 gcccccgggc gacgaggtcg ccgaccgtga ttccgtcggg cgccgtcggc gcctggggca
     4141 ggatcgccac acgcctggcc acccgccgcg gcggcagacc tgcgatgttc gccccgtcga
     4201 gcagcaccgt gccgccctgc ggcgtcaaga gccggtcgag accgcgcaga agggtcgact
     4261 tgccgcttgc gttcgcgccg acaatcgccg tgatcgcccc ggggatgacc gccagatcga
     4321 ggccgtcgat aatgggctcg ccgccgtagc cgagccgcac ctgccgggcc tcgagcgtgg
     4381 cgtgtgccgc cccggcgggc cgcgcccggc tcgtcggcgc ggcggtcgcc ggcttctctg
     4441 tcgccttcgt catcccgcca ccccttcttt cactcgtttt cactcggccg gagcccttcg
     4501 cctgttccgc acgtttcctg ccgtgccgca gccctcaccc gcgccggtgt gtggcgacga
     4561 cgagaatcag gaggaacggc gcgccgagca tgcccgtcac cacgccgacg ggcagcgcga
     4621 cgttatcggg caggctcgcg ctgacgagct gcgcgccgag catgatgagc acgccgacca
     4681 gggcggcggt cgtgacgggg gcccggccgc ggcccgcgcg ccgggcgatc gcgccggcca
     4741 ggaaggcgac gaacgcgacc gggccggcga cggccgtcgc ggccgccgag agcagcacgc
     4801 cgacgacgag cacgaggagc ttggtgcgtg tcacgtcgag ccccagcccg gccgcggagt
     4861 cgt
//
LOCUS       DASTNL010000170         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1048193, whole genome shotgun sequence.
ACCESSION   DASTNL010000170 DASTNL010000000
VERSION     DASTNL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048193"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFQ96_05265"
     CDS             complement(<1..276)
                     /locus_tag="VFQ96_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0257233.1"
                     /translation="MQFGIFSVSDVTRDPVTGVTPSEAERIQDTLVIAQHAEEVGLDV
                     FAIGEHHNPPFWSSSPTTTLAYIAARTKRLIVSTSTTLITTSDPVKVA"
     gene            complement(412..2604)
                     /locus_tag="VFQ96_05270"
     CDS             complement(412..2604)
                     /locus_tag="VFQ96_05270"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU0257234.1"
                     /translation="MLQRTHMQAGPETADGGLRVLDLDITGMTCASCAARIERSLGKL
                     DGVDASVNLIFERAHVTLPEATTDDEIIAAVQGSGYGASVRHLDPVSDPRGLRRRLLV
                     SAILACPVVGISMIMPWQFMGWQWLVAALALPIVIWGAWPFHAAAVRAARHRGSTMDT
                     LVSLGVAAATLWSLWSLAAAVWGGYPQMPGHVYFEVSATVTVFLLAGRVAEAASRRRA
                     SSALHALVALGAKEATVLRVDGDGRESEEERDVAALVVGDLFVVHPGERIATDGVIER
                     GHSAVDESMLTGEPVPLEVGPGDAVTGATVNASGRLVVRVTRVGEHTTLAQIADLVER
                     AQTGKAPVQRLADRVSAVFVPVVVLIAAATLVGWLLATGDASAALTAAVAVLVIACPC
                     ALGLATPTAIAVGTGRGSQLGIVIRGPEILESTRRIDTVLIDKTGTVTEGVLSVASTD
                     LDEETLALTAAAESGSEHPVGRAIVAHVGGPLAAPERFRSHPGGGVEARVAGRAVLVG
                     RPSWIAERLGVAVASPDAGGPVGTEVAVAVDGVLAGHVVLTDRIKAGSAEAVRALHAL
                     GLRTVLLTGDNARQAREVADRVGIEQVLAECLPEEKARAIAEAQRQGAVVAMVGDGVN
                     DAAALATADLGLAMGTGADAAIEAGDITLMRGDLRVAADAVRLSRRTLRIIKQNLFWA
                     FAYNVVAIPLAAFGILNPMVAGAAMAFSSVFVVMNSLRLYDFWPVREA"
     gene            complement(2639..2953)
                     /locus_tag="VFQ96_05275"
     CDS             complement(2639..2953)
                     /locus_tag="VFQ96_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU0257235.1"
                     /translation="MPAAEAGVSRPPYGYAKDKAAYLRRLRRIEGQVRGVSRMLDEDH
                     YCIDILTQISAITSALHAVGVGLLEEHIGHCVLDAAKVSAEAGRAKVEEASAAVARLV
                     RS"
BASE COUNT          486 a         1145 c         1073 g          450 t
ORIGIN      
        1 ggcgaccttg accgggtcgc tggtcgtgat gagcgtcgtc gatgtggaga cgatcaggcg
       61 tttcgtgcgc gccgcgatgt aggcgagcgt cgtcgtcggc gacgacgacc agaacggcgg
      121 gttgtggtgc tcgccgatgg cgaagacatc gagccccacc tcctcggcgt gctgggcgat
      181 gacgagggtg tcttggattc gctcggcctc gctcggtgtg acccctgtca ccgggtcgcg
      241 tgtcacgtcg ctgacactga aaattccgaa ctgcatcccg gtgctcggtg tttcgttcac
      301 ggtctcgtcc attcctgtcg cggtcggcaa ggtatatgca aatgcataca cctcatccaa
      361 cacgaccccg ccgggattat tccaccacgc gacgacgacc gcgcagcccg ctcacgcctc
      421 gcgcaccggc cagaaatcat agagccgcag gctgttcatg acgacgaaga ccgagctgaa
      481 ggccattgcc gcccccgcca ccatcgggtt caggatgccg aaggcggcca acggaatcgc
      541 gaccacgttg taggcgaagg cccagaagag gttctgcttg atgatgcgaa gcgttcgcct
      601 ggagagccgc acggcgtccg ccgccacccg caggtcgccg cgcatcagcg tgatgtcgcc
      661 ggcctcgatg gccgcgtcgg cgcccgtgcc catcgcgagc cccaggtctg ccgtcgcgag
      721 ggccgcggcg tcgttcacgc cgtcgcccac catggcgacc accgcaccct gccgttgcgc
      781 ctcggcgatg gcacgcgcct tctcctcggg caggcactcc gccagcacct gctcgatgcc
      841 gacccggtcg gccacctcgc gcgcctgccg tgcgttgtcg ccggtgagaa gcaccgtgcg
      901 aagcccgagg gcgtgcaagg cacgcacggc ctcggccgag cccgccttga tgcggtcggt
      961 caagacgaca tgtccggcga gcaccccgtc tacggccacg gccacctcgg tgccgaccgg
     1021 cccgccggca tcgggcgagg ccacagccac gccgagtcgt tcggcgatcc acgagggccg
     1081 gccgaccagc acggcccgcc cggcgacgcg cgcctccacg ccgccgcccg ggtggctccg
     1141 gaacctctcc ggcgcggcca gcggcccgcc gacgtgcgcc acgatcgccc gccccaccgg
     1201 gtgctcgctg cccgactcgg ccgcggcagt gagcgcgagc gtctcctcgt cgaggtctgt
     1261 cgaggcgacg gagagcacgc cttcggtcac ggttcccgtc ttgtcgatca agaccgtgtc
     1321 gatgcgacga gtgctctcca ggatctcggg cccgcggatg acgatgccga gctgtgagcc
     1381 gcgccccgtc ccgacggcga tcgcggtcgg tgtcgccagc cccaaggcac aggggcaggc
     1441 gatgacgagc accgcgacgg ccgcggtcag cgcggccgag gcgtcacccg tcgccagcag
     1501 ccagccgacc agggtggcgg cggcgatgag gacgacgacg ggcacgaaga ccgccgagac
     1561 gcgatcggcg agacgctgca cgggcgcctt accggtctgc gcccgctcga cgaggtcggc
     1621 gatctgcgcc agcgtcgtat gctctcccac ccgggtcacc cgcacgacga gccgtcccga
     1681 ggcgttcacc gtcgcgccgg tgaccgcgtc gcccgggccg acctcgagag gaaccggctc
     1741 acctgtcagc atcgactcgt cgaccgcgga gtgcccgcgt tcgatgacgc cgtccgtggc
     1801 gatccgctca ccggggtgaa cgacgaagag gtctcccacg acgagtgccg cgacgtcgcg
     1861 ttcctcttcg ctctctcgcc cgtcgccgtc cacccgcaag acggtcgcct ccttggcgcc
     1921 gagcgccacg agtgcgtgca gcgcggaact cgcgcggcgc cgcgacgcgg cctcggccac
     1981 gcggccggcg agaaggaata cggtgacggt cgccgagacc tcgaagtaga cgtgcccggg
     2041 catctgcggg tatccgcccc ataccgcggc ggcgaggctc cacaaagacc acagggtggc
     2101 ggccgccacg ccgagagaga cgagcgtgtc catcgtggaa ccgcggtggc gggccgcccg
     2161 cactgcggcc gcatggaacg gccaggcgcc ccagatgacg atcggcaacg cgagggccgc
     2221 gacgagccac tgccatccca tgaactgcca cggcatgatc atcgagatgc ccaccaccgg
     2281 gcacgccaag atcgccgaga cgaggaggcg gcggcgcagc ccccgcgggt cggagacggg
     2341 gtcgagatgg cgcaccgagg caccgtagcc actgccttgc acggccgcga tgatctcgtc
     2401 gtccgtcgtc gcctcgggca gggtcacatg ggcgcgttcg aagatcaggt tgaccgaggc
     2461 atcgacgccg tcaagcttgc cgaggcttcg ctcgatacgg gccgcgcacg aggcgcaggt
     2521 catgcccgtg atgtcgaggt cgagaacgcg taaccccccg tcggcggttt ctggcccggc
     2581 ctgcatgtgc gtgcgttgca gcatcgactt cgtcctctct cgtcgccggt cgttcccgtc
     2641 agctgcgcac cagccgggcg acggcggccg aggcttcctc aaccttggcc cgcccggcct
     2701 cggccgagac cttggcagca tcgaggacgc aatgcccgat gtgctcctct aaaaggccca
     2761 cgccgacggc gtgcaacgcg gaagtgatgg cggagatctg cgtcaagatg tcgatgcagt
     2821 aatggtcttc gtcgagcatg cgcgagaccc cgcgcacctg cccctcgatg cgccgcagtc
     2881 tgcgcaagta ggccgccttg tctttggcgt agccatacgg cggccggctg acgccggcct
     2941 cagcggccgg cagcgcctcg ctcgactctg cgtcgaaggg cgcgcctccc tcgccggcga
     3001 aagcgtgctc gggcccgacg tcatccgcgt gggcggccgg ggccgcctct tcggccgctc
     3061 gggccgcctc cgccatcgcg ctcatgaggg aaacgatacc ccggtacggt atatcgtgcg
     3121 agcaacggga gaaggggtgt ggggcagacg cccc
//
LOCUS       DASTNL010000171        10187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_127995, whole genome shotgun sequence.
ACCESSION   DASTNL010000171 DASTNL010000000
VERSION     DASTNL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127995"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VFQ96_05280"
     CDS             complement(<1..984)
                     /locus_tag="VFQ96_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0257236.1"
                     /translation="MKALRLHAYGESPVIEQVAEPTVVGPWDVIVQVGAAGLCRTDLH
                     IIEGQWDPIQHPVLPYVLGHENAGWVREVGSAVTNVAVGDAVIMHPITSCGLCPACRV
                     GDDSHCAHSTFPGINADGGMAEFLRTNARAVVRLGSGLQPADVAALADAGLTAYHAVR
                     KAASLLFPGTHAVVIGAGGLGHIGIQVLAAITAAQITVIDRSEEALELAGQIGADHTI
                     LAGDDEDLRRHVREITGGGADVVFDFVGERGTELLAPRLLRDGGFQYVIGYGGAVHIP
                     TIDIISREISVIGNLVGTYRDLVELMTLTEQHKVTLHTRRYALDDALQAIDD"
     gene            1184..2374
                     /locus_tag="VFQ96_05285"
     CDS             1184..2374
                     /locus_tag="VFQ96_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0257237.1"
                     /translation="MERTELLQVLSPEALKMLDELPPYGGLDILRTEERLRAAGHDAA
                     QVAAVLTQARLRAKAEAKLGAFARRMLFTEAGLQQATRLRVAALHAGRFHAAGLRRVA
                     DLGCGIGADALALAGLGLQVAAIEADEATAALAAYNLAPFPNARVVHDRAEDADLSDR
                     EGVWLDPARRTTGRHPRRLRDPAAFSPPLDFALGLAARLPAGVKLGPGVERGVIPDDV
                     EAQWVSVAGEVVEMTLWSRQLARQGVRRAALVLHDNGADELTAEGDAPDVASAALGDY
                     LYEPDGAVIRARQIGPLADRLGARMLAPHIAYLTSDRAVSTPFANCFRVAETLPFSVR
                     QLTKALRARGIGSLEIKKRGVDLDPAALRRALRLHGPNEATLILTRDRDGDHLAVLAH
                     RMRA"
     gene            complement(2460..3530)
                     /gene="tsaD"
                     /locus_tag="VFQ96_05290"
     CDS             complement(2460..3530)
                     /gene="tsaD"
                     /locus_tag="VFQ96_05290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0257238.1"
                     /translation="MAASEPLVIGIESSCDETGIGIVRGTRLLANTISSSMEQHARYG
                     GVVPEVAARAHLEALVPALRQAVDEAGIGLREVTAIAVTSGPGLAGALMVGVGAAKAL
                     AVALGVPIYAVNHLVGHVGADVLDAEGGPGRALDAPTIALLVSGGHTSLLHVRDLVDD
                     VELLGETLDDAAGEAFDKVARILGLPYPGGPQIDRVAVDGDPNAIRFPRGLTKPKDMA
                     EHRYDFSFSGLKTAVARWVEAREDAGLPVPVADVAASFRQAVVDVLVTKAVDACRRRD
                     VPRLLLGGGVVANARLREEAARRAAEAGIALRIPALSLCTDNGAMIAALGAQLIMAGH
                     GPSTLDFGADSTLPVTTIQDLG"
     gene            complement(3530..4060)
                     /locus_tag="VFQ96_05295"
     CDS             complement(3530..4060)
                     /locus_tag="VFQ96_05295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0257239.1"
                     /translation="MTTDWSLRPAGPHDLGTLAPLEKRIFSGDAWSEETLAAELGSPH
                     THYVIAFEGAFPGGRVPGRQDVPAGRREAAGYGGLFAPRGSGDADIQTLAVRQECRGR
                     GLGRLLLQALLAEASRRRAARVFLEVRADNPVAQHLYETAGFTRQGIRRHYYQPDDVD
                     AFVLRLELAGAGKGDD"
     gene            complement(4060..4674)
                     /gene="tsaB"
                     /locus_tag="VFQ96_05300"
     CDS             complement(4060..4674)
                     /gene="tsaB"
                     /locus_tag="VFQ96_05300"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU0257240.1"
                     /translation="MLLAIDTSAGSSVAVVDEAAGVIAECATEDTMRHAETIGAFIRD
                     CLAEAHASPAGLDGVAVGMGPGPFTGLRVGIAAARVFALGAGIPVLPVVSHDAIAFAR
                     YTGGVTGDFLVVTDARRREAYWSAYRGVLGGLPRRVDGPGLARPDALPHQELPRLEAH
                     AVSAGAVGLLAERLRRLGRPLADDQPLYLRSPDVTPAARRKRVR"
     gene            complement(4736..5263)
                     /gene="tsaE"
                     /locus_tag="VFQ96_05305"
     CDS             complement(4736..5263)
                     /gene="tsaE"
                     /locus_tag="VFQ96_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU0257241.1"
                     /translation="MRVFGTHTVPTGARMESLGEALAALLLPGDIVLLTGPLGAGKTT
                     LTRGIGRGLGVRGPVTSPTFVLARTHPSLRGGPPLVHVDAYRLSDALELDDLDLDFAR
                     SVTVVEWGAGLLDGIAESWLDVVIDRPTGGVAQGSGVAGGDDLADEVEEPRLVTVRGR
                     GPRWRHAAAPGGDPS"
     gene            complement(5260..6483)
                     /locus_tag="VFQ96_05310"
     CDS             complement(5260..6483)
                     /locus_tag="VFQ96_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0257242.1"
                     /translation="MTAAATLPPLPAGVLRQAVTDEAAIASNVAALRGVLDVRHLMVV
                     VKADGYGHGLVPAARAALTGGADWLGVADVDEALTLRSAGVMAPVLAWLHGPGSDFPA
                     AAAAGIDLALSSLAQLDAAAKAARAAAGGRAGVQLALDTGLNRNGAPREAWAALFRRA
                     AELEAAGLVRVRGILSHVSNTSEADDLAQLALFREAVGRARGVGLRPELLHLAATAAA
                     LTLPPTRLTMVRIGIAAYGLTPFEAGFDDLGASTAGGGPPGAGTDDPFTRLGLTPALT
                     LRAPVVAVRRVAAGEGVSYGYTYRTAGPTSLALIPLGYADGIPRSASNRGVVTVGGVR
                     YPQVGRIAMDQFIIEAGGRDPAVRVGDEAIVFGDPARGAVGVGEWARAAGTIGYEIVT
                     RLGRRVGRTSVGGAA"
     gene            complement(6480..6839)
                     /locus_tag="VFQ96_05315"
     CDS             complement(6480..6839)
                     /locus_tag="VFQ96_05315"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU0257243.1"
                     /translation="MIAGIGVDVVDVQRFERAVSRTPRLLTRLFAQGEQGLPVRSLAA
                     RFAAKEALVKALGGDAGLGWPDLEVVSDPEGRPAFVSRGRLAGVLAARGVRRAHLSLT
                     HDAGIACAFVVLDGSAS"
     gene            6954..7982
                     /gene="coaA"
                     /locus_tag="VFQ96_05320"
     CDS             6954..7982
                     /gene="coaA"
                     /locus_tag="VFQ96_05320"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU0257244.1"
                     /translation="MAEAPPCHTAARPGSGQAPARPVRPHPGPAETDHGQISPFLEIA
                     RSRWAELAENAVLPLTETELVRLRSLGDPLSMREVQRVYLPLTRMLNLYVSATKNLHR
                     ATEGFLGERTGPTPFVIGVAGSVAVGKSTISRLLRTLLARWENTPRVELVTTDGFLLP
                     NAELERRGLMPRKGFPESYDRRALLRFLTAVKSGAAEVRAPFYSHLSYDIVPDSEIVV
                     HQPDVLILEGLNVLQPAGGGRSRLAVSDLFDFSVYVDAPRRDIERWYEERFLKLQRGA
                     FMNPQSYFHRYADLSETEARERARQIWHETNEPNLMQNIRPTRSRAKLVLRKGGDHSV
                     HSVLLRKL"
     gene            complement(7984..9348)
                     /gene="glmM"
                     /locus_tag="VFQ96_05325"
     CDS             complement(7984..9348)
                     /gene="glmM"
                     /locus_tag="VFQ96_05325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0257245.1"
                     /translation="MARLFGTDGVRGLANGGLTADLALHLAQAAAAVLTRGRSAEARR
                     AEGRRPLAVVARDPRVSGEFLIAAVSAGLASSGIDVLDAGVIPTPATAFLIADRGADF
                     GVMISASHNPAPDNGIKIFARGGTKLPDVVEDRIEAAMAGPKLTPTGADVGRISRFAD
                     AEDRYVLHLLASLPHRLDGVHVVLDCANGAASGVSPEAFRDAGAALTVIGDDPDGINI
                     NDGVGSTHIDRLREAVVAAGADVGIAHDGDADRCLAVDHEGRVVDGDQIMAILAVSMA
                     ERGELDKNTLVTTVMSNLGLRLAMEEHGIRTVQTKVGDRYVLQEMDEHAYALGGEQSG
                     HVILRHHATTGDGLLTGLHLVAEVARTGKSLASLAAVMTVFPQTLINVPGVDKARLDG
                     DEVIAHAVASVEDVLGESGRVLLRPSGTEPMVRVMVEAAEQAVADEHAGALAAVVRER
                     LATA"
     gene            complement(9391..9876)
                     /gene="rpsI"
                     /locus_tag="VFQ96_05330"
     CDS             complement(9391..9876)
                     /gene="rpsI"
                     /locus_tag="VFQ96_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0257246.1"
                     /translation="MAKIADQVESLGSFSTETPATAETAQTPRPVLSVPGQAVGRRKQ
                     AIARVRLVPGTGTITVNGRAFADYFPNKLHQQLITDPFTLLQLQDSYDVVASITGGGP
                     SGQAGALRLGIARALNVIDAEHNRPGLKKAGFLSRDARVKERKKAGLKKARKAPQYSK
                     R"
     gene            complement(9932..>10187)
                     /gene="rplM"
                     /locus_tag="VFQ96_05335"
     CDS             complement(9932..>10187)
                     /gene="rplM"
                     /locus_tag="VFQ96_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU0257247.1"
                     /translation="VTGNKAERKVIYRHSGYPGGLRATAYGDLLEKNPVRAVERAVRG
                     MLPKNSLGRAQFRKLKVYAGPEHPHAAQQPKQYTFDQVAQ"
BASE COUNT         1457 a         3666 c         3677 g         1387 t
ORIGIN      
        1 gtcgtcgatc gcctgcaacg cgtcgtcgag ggcgtaacgc cgggtgtgca gcgtgacctt
       61 gtgctgctcg gtgagcgtca tgagctccac aagatcgcga taggtgccca ccaggttgcc
      121 gatcacgctg atctcacggg agatgatgtc gatcgtcgga atatgcaccg cgccgccata
      181 gccgatcaca tactggaacc cgccgtcgcg aaggagccgc ggcgccagaa gctccgtgcc
      241 gcgttcgccg acgaagtcga agaccacgtc ggcgccgccc ccggtgatct cacgcacgtg
      301 gcgccggagg tcctcgtcgt cgccggccag gatcgtgtgg tccgcgccga tctggccggc
      361 cagctccaaa gcctcttccg accggtcgat gaccgtgatc tgggctgcgg tgatcgctgc
      421 caacacctga atgccgatgt ggccgagacc gcccgcgccg atcacgacgg catgggtgcc
      481 ggggaagagc aggctcgcgg ccttccgcac cgcgtggtaa gccgtgagcc cggcatccgc
      541 caacgccgcc acgtccgccg gctggaggcc cgaccccagc cttacgaccg cacgtgcgtt
      601 ggtgcggagg aactcggcca tgccgccgtc ggcgttgatg ccggggaacg tggagtgggc
      661 gcagtgcgag tcgtcaccga cccggcaggc ggggcacagg ccgcagctgg tgatgggatg
      721 catgatcacg gcgtcgccga cggcgacgtt cgtcacggcg ctgccgacct cgcgcaccca
      781 gccggcattc tcgtgcccga gcacgtacgg cagcacgggg tgctggatcg gatcccactg
      841 cccttcgatg atgtgcaggt cggtgcggca caggcccgcg gcacccacct gcacgatgac
      901 gtcccacggc ccgacgaccg tgggctccgc cacctgctcg atgaccgggg actccccgta
      961 agcgtgcagc cgcagtgcct tcatcgcatg cctccgtacc cccgcgccct cccggagccc
     1021 tccttgtctc ggggcactcc gaacgagacg ccgttacgcc gttatcgtcg tgaccacagc
     1081 atgtgcgcaa ggcgcccgga gcggtagacg cgttctcgac aggcgataac gcgccgccgg
     1141 gacggcagat gacgcggagg gccggggcga tagggtaacg cgggtggaga gaaccgagct
     1201 acttcaggtg ctctcgcccg aggccctgaa gatgctggac gaactgccgc cctacggcgg
     1261 cctcgacatc ctgcgcaccg aggagcggct gcgcgcggcc ggccacgacg ccgcgcaggt
     1321 cgcggccgtg ctgactcagg cgaggctccg ggccaaggcg gaggcgaagc tcggcgcctt
     1381 cgcacgtcgc atgctcttca ccgaggcggg actgcagcag gcgacccgac tgcgggtggc
     1441 ggcgctgcac gccgggcgct ttcacgccgc cggccttcgc cgggtggcgg atctcggctg
     1501 cggcatcggc gccgacgcgc tggccctggc ggggctcggc ctgcaggtcg cggcgatcga
     1561 ggccgacgag gcgaccgccg cgcttgccgc ctataacctt gcgccgttcc cgaatgcccg
     1621 ggtcgtgcac gaccgcgcgg aagatgccga cctctccgac agggagggcg tctggctcga
     1681 ccccgcccgc cgcaccacgg gcagacaccc gcggcgactg cgtgacccgg cggcgttctc
     1741 cccgccgctc gacttcgcgc tcggcctcgc cgcccggctc cctgccggcg tcaagctcgg
     1801 cccgggcgtc gaacgcggcg tcattcccga cgacgtggag gcgcaatggg tgagcgtggc
     1861 cggcgaggtc gtcgagatga cgttgtggtc gcggcagctg gcccggcagg gcgtgcgtcg
     1921 ggccgcactc gtgttgcacg ataacggcgc ggacgagttg acggcggagg gcgacgcgcc
     1981 ggatgtggcg agcgccgccc tcggcgacta cctgtacgag cccgacggtg cagtgatccg
     2041 cgcacggcag atcggccccc tcgccgaccg gctgggggcc aggatgctcg cgccgcacat
     2101 cgcctacctc acctccgacc gcgcggtgtc gacgcccttc gccaactgct tccgcgtcgc
     2161 ggagacgctg ccgttctcgg tgcggcagct gaccaaggct ctgcgtgccc gcggcatcgg
     2221 gtcgctggag atcaagaagc gcggcgtcga cctcgacccg gcggcgttgc ggcgcgccct
     2281 gcgcctgcac gggccaaacg aggccaccct catcctcact cgcgatcgcg acggcgatca
     2341 cctggcggtg ctcgcacacc gcatgcgcgc gtagcaggag aaagccccac cgccacccga
     2401 gaagagcggg cacggcggga aatcacacgt ctgaggtcgg ccgaggactc agacggcagt
     2461 cagccgaggt cttggatcgt ggtcaccggc aacgtcgagt cggcgccgaa gtcgagggtc
     2521 gacgggccgt gcccggccat gatcagctgc gcgccgagcg cggcgatcat cgcgccgttg
     2581 tcggtgcaga gcgacagggc cgggatgcgc agcgcgatgc cggcctccgc cgcacgcctg
     2641 gcggcctcct cgcgcagccg ggcgttggcg accacgcccc cgcccaggag caggcggggc
     2701 acgtcgcggc gccggcaggc gtcgaccgcc ttcgtgacga gcacgtcgac gaccgcctgg
     2761 cggaaactcg ccgccacgtc ggcgacggga accggcaggc cggcgtcttc ccgggcctcc
     2821 acccagcgcg cgacggcggt cttcagaccg gagaacgaga agtcgtagcg gtgctccgcc
     2881 atgtctttgg gcttcgtcaa gccgcgggga aaccggatcg cgttcgggtc gccgtcgacc
     2941 gccacgcggt cgatctgcgg gccgcccggg tacggcaagc cgaggatgcg ggcaaccttg
     3001 tcgaacgcct cgccggccgc gtcgtcaagg gtctcgccga gcagctcgac gtcgtcgacc
     3061 aggtcgcgaa cgtgcagcag gctggtgtgc ccgcccgaga cgagcagcgc gatcgtcggc
     3121 gcgtcgagcg cgcgccccgg gccgccctcg gcatcgagca cgtcggcgcc gacgtggccg
     3181 acgagatggt tgacggcgta gatcggcacg cccaatgcca cggcgagcgc cttggcggcg
     3241 ccgaccccga ccatgagggc gccggcgagc ccgggaccgc tcgtcacggc gatcgccgtg
     3301 acctcgcgaa ggccgatgcc cgcctcgtcg acggcctgcc gcagcgccgg cacgagcgcc
     3361 tcgaggtggg cgcgggccgc aacctcgggc acgactccgc cgtaacgggc gtgctgctcc
     3421 atcgaggagg agatggtgtt ggcgagcagg cgcgtgccac gcacgatgcc gatgcccgtc
     3481 tcgtcgcacg agctctcgat tccgatgacg agcggttcgc ttgcggccat cagtcgtctc
     3541 ccttcccggc cccggccagt tcgagacgca ggacgaaggc gtcgacgtcg tcgggctggt
     3601 agtagtgccg ccggatgccc tgccgggtga agcctgccgt ctcgtagaga tgctgggcga
     3661 cgggattgtc ggcccgcacc tcgaggaaca cgcgggccgc gcggcggcgg gacgcctcgg
     3721 cgagaagcgc ctggagcagc agccggccaa ggccgcgacc ccggcactcc tgccgcaccg
     3781 cgagcgtctg gatgtcggcg tcgcccgagc cgcgcggcgc gaagagcccg ccgtagccgg
     3841 ccgcctcccg acgcccggca ggcacgtcct gccgcccggg cacgcgcccg cccgggaagg
     3901 cgccctcgaa ggcgatcacg tagtgggtgt gcggggagcc gagttccgcg gccaaggtct
     3961 cctctgacca ggcgtcgcca gagaagatgc gcttctccag cggggccaga gtgccgaggt
     4021 cgtgaggccc cgcggggcgc agcgaccagt cggtcgtcat caccgcaccc gcttgcgccg
     4081 cgccgccggg gtgacgtcgg gcgaacgcag gtagagcggc tgatcgtcgg cgagcgggcg
     4141 gccgagccga cgcagtcgct cggcgaggag cccgacagcc ccggcggaga cggcgtgcgc
     4201 ctcgaggcgc ggcagctcct ggtgcggcag agcgtcgggc cgcgcgagcc ccggcccgtc
     4261 gacccggcgc ggcagcccgc caagcacgcc gcggtaggcg gaccagtacg cctctcggcg
     4321 gcgcgcgtcg gtgacgacga ggaagtcgcc cgtcacccca cccgtgtagc gtgcgaaggc
     4381 gatcgcatcg tggctgacga ccggcaagac ggggatcccg gcaccgaggg cgaagacccg
     4441 ggcggcggcg atgccgaccc gcaggcctgt gaacgggccc ggccccatgc cgacggcgac
     4501 gccgtcgagg ccggccggcg aggcgtgcgc ctcggccagg cagtcgcgga tgaaggcgcc
     4561 gatagtctcg gcatgccgca tcgtgtcctc ggtcgcacac tcggcgatga cgccggcggc
     4621 ctcgtcgacc acggcgacgc tggagccggc ggaggtatcg atggcgagga gcactcgccc
     4681 agcctattcc gacgcctcgg gcgcgcaggt cctgcgcccc cgcgccggcg cctcgtcagg
     4741 acgggtcgcc tccgggcgcc gcggcgtggc gccagcgggg cccgcggccg cgcaccgtga
     4801 cgaggcgcgg ctcctcgacc tcgtccgcga ggtcatcccc gcccgccacg cccgacccct
     4861 gtgcgacgcc gccggtcggc cggtcgatga ccacgtcgag ccacgactcc gcaatgccgt
     4921 cgagaaggcc cgcgccccac tcgacgaccg tcaccgagcg ggcgaagtcg aggtcgaggt
     4981 cgtcgagttc cagcgcgtca ctcagccggt aggcgtcgac atgcacgagc ggcgggccgc
     5041 ccctgaggct cggatgcgtg cgtgcgagca cgaaggtggg gctcgtgacc ggcccgcgca
     5101 ccccgagccc ccggccgatg ccgcgcgtca acgtggtctt gccggcgccc agcggcccgg
     5161 tgagaagcac gatatccccg ggcagcagga gggcggcgag agcctcgccg aggctttcca
     5221 tgcgggctcc ggtcggcacc gtgtgcgtgc cgaataccct catgcggcac ccccgacgct
     5281 cgtgcggccg acgcggcgac ccaaccgggt gacgatctcg tagccgatcg tgcctgccgc
     5341 ccgggcccac tcgccgacgc cgacggcgcc tcgtgccggg tcgccgaaga cgatggcctc
     5401 gtcgccgacc cgcacagcgg gatcgcggcc ccccgcctcg atgatgaact ggtccatcgc
     5461 gatgcggccg acctgcgggt aacgcacgcc gccgaccgtg acgacacccc ggttcgaggc
     5521 gctgcgcggg ataccgtcgg cgtagccgag cggaatgagc gcgaggctcg tcggcccggc
     5581 cgtgcgatag gtgtacccgt acgagacgcc ctcgccggcg gccacgcgcc gcaccgccac
     5641 gaccggcgcg cgcagggtca gcgccggcgt gaggccgagc ctcgtgaacg ggtcgtccgt
     5701 gccggcgccc ggcggcccgc ccccggcagt cgaggcgccg aggtcgtcga agcccgcttc
     5761 gaatggtgtg aggccgtagg cggcgatgcc gatgcggacc atcgtcagcc tggtcggcgg
     5821 cagggtcagt gccgcggcgg tggccgcgag gtgcagcagt tcggggcgca gaccgacgcc
     5881 ccgcgcccgg ccgaccgcct cacgaaaaag cgcgagttgg gcgaggtcgt ccgcctccga
     5941 cgtgttggag acgtggctga ggatgccccg cacccgcacg aggccggccg cctcgagctc
     6001 ggcagcccgg cgaaaaagcg ccgcccacgc ctcacgcgga gcgccgttcc gattgaggcc
     6061 cgtgtcgagg gcgagctgca cgccggcccg gcccccggcg gcagcccgcg ccgccttcgc
     6121 cgccgcgtca agctgggcga gggaggagag cgccaggtcg atcccggcgg ccgcagcggc
     6181 cgggaagtcg cttcccggcc cgtgcagcca ggcgagcacg ggcgccatga ccccggcgga
     6241 gcgcagggtg agtgcctcgt cgacgtccgc cacgccgagc cagtcggcgc cacccgtcaa
     6301 cgccgcgcgg gcggccggca cgaggccgtg cccgtagccg tcggccttca cgacgaccat
     6361 caggtgccgc acgtcgagaa cgcctcgcaa ggcggcgacg ttgctcgcga tcgccgcctc
     6421 gtcggtgacg gcctggcgca agacgccggc gggcagaggc ggcagggtgg cggccgccgt
     6481 cacgacgcgc tcccgtcgag cacgacgaag gcgcaggcga tgcccgcgtc gtgggtgagg
     6541 ctcaggtgcg cccggcgcac acctcgcgcg gcgaggacgc cggcgagacg gccgcgcgag
     6601 acgaaggccg gcctgccctc cgggtcggag acgacctcca gatcgggcca gccgaggccc
     6661 gcgtcacccc cgagcgcctt caccagcgcc tctttcgccg cgaaacgggc ggccagcgag
     6721 cgcaccggca gcccttgttc gccctgggcg aacaagcggg taagaagcct cggggtgcgg
     6781 ctgacggcgc gttcgaaacg ctgaacgtcg acgacgtcga ccccgatacc cgcgatcacg
     6841 cgacgatcct accggccacg ccctgcccgg ccgggcgtcg cgacacggca cggagggctt
     6901 ctgcggcgct cgccgggcgg cggcccgcac ccgaggccct aaactggtcg cctatggcag
     6961 aggctccgcc gtgtcacacc gcggcccgtc ccggttccgg ccaggcaccg gcacggcccg
     7021 tccggccgca tcccggcccc gccgagacag atcacggcca gatcagcccg ttcctggaga
     7081 tcgcgcgcag ccgctgggcg gaactggccg agaacgcggt gctgcccctc accgagacgg
     7141 agctcgtcag gctgcgcagc ctcggcgacc cgctcagcat gcgagaggtg cagcgggtgt
     7201 atctgccgtt gacccgcatg ctgaacctct acgtctcggc cacgaaaaac ctgcatcgcg
     7261 cgacggaggg cttcctcggc gagcgcaccg gcccgacccc cttcgtcatc ggcgtcgcgg
     7321 gctccgtcgc cgtcggcaag tcgacgatct cccggctcct tcgcaccctg ctcgcgaggt
     7381 gggagaacac gccccgggtg gagctcgtga cgacggacgg gttccttctc ccgaacgccg
     7441 agctggagcg gcgaggtctg atgccgagga aggggttccc ggagtcgtat gaccggcgtg
     7501 cgctgctgcg gttcctgact gccgtgaagt cgggcgccgc cgaggttcgc gcgccgttct
     7561 actcgcatct cagctacgac atcgtgccag acagcgagat cgtggtccac cagcctgacg
     7621 tcttgattct ggagggcctg aacgtgttgc agccggccgg cggcggccgc agccggctgg
     7681 ccgtcagcga ccttttcgac ttcagtgtct acgtcgacgc gcccaggcgc gacatcgagc
     7741 ggtggtacga ggagcgtttc ctcaagctgc aacggggtgc cttcatgaac ccgcagtcgt
     7801 atttccaccg ttacgccgac ctctccgaga cggaggcccg cgagcgtgcc cggcagatct
     7861 ggcacgagac gaacgagccg aacctgatgc agaacatccg ccccacgcgc tcccgtgcga
     7921 agctcgtctt gcgcaagggc ggcgaccaca gcgtgcactc ggtgctgctg cggaagctct
     7981 gactcaagcc gtggcgaggc gctcccgcac gacggcggcc aacgcgcccg cgtgctcgtc
     8041 ggcgaccgcc tgctcggcag cctcgaccat gacgcgcacc atcggctccg tgccggaggg
     8101 gcgcaagagg acccggccgc tctcccccag cacgtcctcg acactcgcca ccgcgtgcgc
     8161 gatgacctcg tcgccgtcga gccgggcctt gtcgacgccc ggcacgttga tgagtgtctg
     8221 cgggaagacg gtcatgacgg cggcgaggga ggcgaggctc ttgccggtgc gggcgacctc
     8281 ggcgaccagg tgcagccctg tcaagaggcc gtcacccgtc gtggcgtggt gccgcaggat
     8341 cacatggccg gattgttcgc cgcccaacgc gtaggcgtgc tcgtccatct cttgcagcac
     8401 gtaccggtcg ccgaccttgg tctgcacagt gcggatgccg tgctcctcca tcgccaggcg
     8461 caggccgagg ttgctcatca cggtcgtcac gagtgtgttc ttgtcgagtt caccgcgttc
     8521 cgccatgctc acggcgagga tggccatgat ctgatcgccg tcgacgacgc ggccttcgtg
     8581 gtcgaccgcc aggcaacggt cggcatcgcc gtcgtgtgcg atgccgacat cggcgccggc
     8641 cgcgacgaca gcctcgcgca gccggtcgat gtgggtcgag ccgacgccgt cgttgatgtt
     8701 gatgccgtcg gggtcgtcgc cgatgaccgt gagcgcggcg cccgcgtcac ggaacgcctc
     8761 cggcgacacg cccgaggcgg ccccgttggc acagtccagc acgacgtgca cgccgtcgag
     8821 ccggtgcggc aggctcgcca agaggtgcaa cacgtagcgg tcttcggcgt cggcgaagcg
     8881 tgagatgcgg ccgacatccg cgccggtggg ggtcagtttc gggccggcca tcgccgcctc
     8941 gatgcggtct tcgaccacgt cgggcagctt ggttccgcca cgcgcgaaga tcttgatgcc
     9001 gttgtcgggc gctggattgt gggaggcgga gatcatgacg ccgaagtctg cgccgcggtc
     9061 ggcgatgagg aacgcggtcg ccggcgtcgg gatgaccccg gcatccagca cgtcgatgcc
     9121 ggaacttgca agcccggcgg aaaccgccgc gatgaggaac tcgcccgaca cccgcgggtc
     9181 tcgggcgacg accgcgagcg ggcgccgtcc ctccgcccgc ctggcctcgg cgctgcgacc
     9241 ccgggtgagg acggcagcgg cggcctgcgc caggtgcagc gccagatcgg cggtcaggcc
     9301 accgttggcg aggccgcgca cgccgtcggt gccgaacaaa cgcgccatgc cagcctcctt
     9361 gcgcccgcga gtgtcaccgc caagggtgca tcatctcttg gaatactggg gcgccttgcg
     9421 cgccttcttc aagcccgcct tcttacgttc cttgacgcgg gcgtcgcgac tcagaaaacc
     9481 ggccttcttg aggcccgggc gattgtgctc ggcgtcgatg acgttcaacg cccgcgcgat
     9541 gccgagccgc agcgcgcccg cctggccgga ggggccgccg ccggtgatcg acgcgacgac
     9601 gtcgtagctg tcttgcagct ggaggagcgt gaacgggtcg gtgatcagct gttggtgaag
     9661 cttgttggga aagtagtccg cgaacgcgcg gccgttgacc gtgatggtcc cggtgcccgg
     9721 cacgagacgg acacgggcga tggcctgctt gcgccgaccc accgcctgcc cggggacgct
     9781 gaggacgggc cgcggggtct gggccgtctc tgctgttgcc ggtgtctcgg tcgagaagct
     9841 tccgagactc tcgacctggt ctgcgatctt cgccactgtg gtgtgagtcc ttttctggtg
     9901 aacggtgctg agtgtggtgg ccggcgggtg cctactgggc gacctggtcg aaggtgtatt
     9961 gcttgggctg ttgggcggca tgcgggtgct cggggccggc gtagaccttg agcttgcgga
    10021 actgcgcgcg gcccagagag ttcttcggca gcatgccgcg cacggccctc tcgacagccc
    10081 gcacggggtt cttctcgaga aggtcgccgt aagcggtcgc ccgcaatccc cccgggtagc
    10141 cggagtgccg gtagatgacc ttgcgctcgg ccttgttgcc ggtgacg
//
LOCUS       DASTNL010000172         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_498192, whole genome shotgun sequence.
ACCESSION   DASTNL010000172 DASTNL010000000
VERSION     DASTNL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498192"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2435)
                     /locus_tag="VFQ96_05340"
     CDS             complement(<1..2435)
                     /locus_tag="VFQ96_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU0257248.1"
                     /translation="MPADKRSLTGALGALFGFVCLSAIAGLIVTAAVTPAVAVTGVAT
                     NNTISAFDQLPESLKLDQLSQVTKIYAHDGNAEREIASFYVQNRVEVGWNDISQYVKD
                     AATSGEDPEFYHHGAISVKGTMRAAVSTFLHHDTQGGSTITQQYVKNVLIQECEADNA
                     DNTAKLAACYQNATRQTISRKIQEMKYAIGLEKKYTKRQILLGYLNINGFGGQVYGIQ
                     SAAEYYFGVSAKDLSLPQAASLVAIVQNPDYNRLDRPKNDKINGADNGYQKNKARRDY
                     ILAQMLKYNKITQAQYEKAVQTPVKPKITPQSNGCMAANAYDAGAFCNYIENLIKNDP
                     AFGATSAARFANLRRGGYKIYTTLDLGLQKKAQETLSGYVPAYRAGMDLGAASVGLQQ
                     NTGNILYMVQNRSFNDTPSAAKHHQTSLNYAVDKQYGGSQGFQTGSTYKAFDLIAWLE
                     EGHTLNTVVDASRHLYPQSMFHSSCDTIAGADWNVGNAEGSAGHISVLNATKYSVNTA
                     FARMATQTDLCQIKKSAQALGVHLGDGQPIQSVNPSAVIGTQDIAPLTMAVAYAAIAN
                     NGIVCTPIAIDKIVDGSGANVPTSKTTCTQGIDPAIANAAAYALQTPLTPGGTAATAN
                     PHDGVPMLAKTGTTDYAAQNWLISATTKTAQATWVGNVEAVASSGGTPHYADMRTEFF
                     RGIDGATRAGNNVKFPIVKAITAMLNKRFGGTAFPKIEAKYLNGAQTAVPDVGGMTAS
                     AATTALEKAGFTVATGAAVDGVQPAGTVEKTSPAAGAKAPKGSTVTIYASKGNEKTLP
                     DAGQVIGQTLAQAT"
     gene            2652..3122
                     /locus_tag="VFQ96_05345"
     CDS             2652..3122
                     /locus_tag="VFQ96_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0257249.1"
                     /translation="MASASERLRELGITLPHVPAPVAAYVPAVVSGQVVHTSGQLPFV
                     GGVLPEAGKVGEGEGLVSPEAARGYARQAALNALAAVQSVIGSLDRVVRVVKVTGFVA
                     SAAGFAGQSTVVDGASTLVGEIFGERGRHARSSVGVAELPLGSPVEVELSVEFA"
     gene            complement(3084..>4798)
                     /gene="pabB"
                     /locus_tag="VFQ96_05350"
     CDS             complement(3084..>4798)
                     /gene="pabB"
                     /locus_tag="VFQ96_05350"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU0257250.1"
                     /translation="ARPHPPTPLVWFALTDHPPTRIGPAATSTTATAPATRGAAHAAQ
                     AAVGGWRLDTTRAEYTTRFDAVRAAIAAGETYQCNLSARMTGLACDTTPEALFGYYRS
                     LLRNQGGDNGAWLVTDRFAVASASPESFFEWRGDEIRCTPMKGTSPRGPDSASDEVLR
                     RALLASAKDRAENVMIVDLMRNDLSRIAAPGSVQVPALLACERYATLWQLTSTITARI
                     EREASLAGILAALFPCGSVTGAPKARTMELIAQLETSPRGLYCGAIGWLAPPGHAVRA
                     RFNVAIRTVVVDRTTGRAEYGVGGGITWGSSAEGEFAEILAKTAVLRDPPEEDFSLIE
                     TLREHDGRPVHLTEHLARLRASAHYFGFPLEEDEIRARLAAATAPGNPDDPDNPGDPG
                     NPGNPPRRDGRGGLHGDRRLRIALDRHGRCTITVGAAPKRASRPRTLAIDTPRIDPRS
                     RWLRHKTTLRAVYAAARERHPEADDTVLVNTNGNITETSIANIVVRLAGLWFTPPVAD
                     GCLPGVGRGRLIADGIVQERSLSIADLRRADAIAVVSSLRGWLPAVLADPSGEFDAQL
                     HLDR"
BASE COUNT          633 a         1515 c         1758 g          892 t
ORIGIN      
        1 gtcgcctgcg ccaaggtctg cccgatgacc tggccggcgt cgggcagcgt cttctcgttg
       61 cccttcgagg cgtagatcgt cacggtgctg cctttcggcg ccttcgcgcc ggcggcaggc
      121 gaggtttttt ccaccgtgcc ggccggctgc acaccgtcaa cggcggcgcc cgtggcgacg
      181 gtgaacccgg ccttctccag agccgtcgtc gccgcactcg cggtcatgcc tccgacgtcg
      241 ggcacggccg tctgtgcacc gttcaggtac ttcgcctcga tcttcgggaa cgccgtgccg
      301 ccgaaccttt tgttcagcat cgccgtgatc gccttgacga tcgggaactt gacgttgttg
      361 ccggcccgag tcgcgccgtc gataccgcgg aagaactcgg tacgcatgtc ggcatagtgc
      421 ggcgtgccgc ccgacgacgc cacggcctcg acgttgccca cccaggtcgc ctgcgctgtc
      481 ttcgtcgtcg cgctgatcaa ccagttctgc gccgcgtaat cggtcgtgcc ggtcttcgcg
      541 agcatcggca ccccgtcgtg cggattcgcg gtggccgcgg tgccgcccgg cgtcagcggg
      601 gtctgcagcg cgtacgccgc cgcgttggcg atcgccgggt cgatgccctg cgtgcaggtc
      661 gtcttgctcg tcggcacgtt cgctcccgac ccgtcgacga tcttgtcgat ggcgatcggg
      721 gtgcagacga tgccgttgtt cgcgatggcg gcgtaggcga ccgccatcgt gagcggagcg
      781 atgtcttgcg tgccgatgac ggccgacggg ttcacggatt ggatcggctg gccgtcgccg
      841 agatgcacgc cgagcgcctg ggcgctcttc ttgatctggc acaggtcggt ctgcgtcgcc
      901 atgcgcgcga aggccgtgtt cacggagtac ttcgtcgcgt tcaagaccga gatgtggccg
      961 gcggagcctt ccgcgttgcc gacgttccag tcggcgccgg cgatcgtgtc gcaactgctg
     1021 tggaacatcg attgcgggta gaggtgccgt gaggcgtcga cgaccgtgtt cagggtgtgc
     1081 ccctcttcca accaggcgat gaggtcgaag gccttgtagg tcgatccggt ctggaagcct
     1141 tgcgagccgc cgtactgctt gtcgacggcg tagttcagcg acgtctggtg gtgtttcgcg
     1201 gcgctggggg tgtcgttgaa cgaacggttc tgcaccatgt agagaatgtt gccggtgttc
     1261 tgttgcagac ccacgctggc ggcgccgaga tccatgccgg cgcggtaggc gggcacgtag
     1321 ccgctcagcg tctcctgggc cttcttctgc aggccgaggt cgagggtcgt atagatcttg
     1381 tagccgccgc ggcggaggtt cgcgaagcgt gccgccgacg tcgccccgaa cgccgggtcg
     1441 ttcttgatga ggttctcgat gtagttgcag aaggcgcccg cgtcgtaggc gttcgccgcc
     1501 atgcagccgt tcgactgcgg cgtgatcttc ggcttgacgg gggtttgcac cgccttctca
     1561 tactgtgcct gcgtgatctt gttgtacttc agcatctgcg ccaaaatgta gtcgcgccgc
     1621 gccttgttct tctggtagcc gttgtcggcc ccgttgatct tgtcgttctt cggccggtcg
     1681 agccggttgt agtcggggtt ctgcacgatg gccacgaggc tggcggcttg cggcagcgac
     1741 aggtctttcg cgctcacgcc gaagtaatat tccgcggccg attggatgcc gtacacctgg
     1801 ccgccgaatc cattgatgtt caggtagccg agcagaatct gccgcttggt gtatttcttc
     1861 tccaggccga tcgcgtattt catctcttgg atcttgcggc tgatcgtctg ccgggtcgca
     1921 ttctgatagc aggcggcgag cttggcggtg ttgtcggcgt tgtcggcctc gcactcttga
     1981 atgagcacgt tcttcacata ctgctgcgtg atcgtcgagc cgccttgggt gtcgtgatgc
     2041 aagaaggtcg acacggccgc ccgcatcgtg cccttcacgc tgatcgcgcc gtgatgatag
     2101 aactccgggt cttcgcccga ggtagcggcg tctttcacat actgcgagat atcgttccac
     2161 ccgacctcga cacggttttg cacgtagaac gaggcgatct cgcgctcggc gttgccgtca
     2221 tgcgcgtaga tcttcgtcac ctgggagagc tggtcgagct tcagcgactc gggcagctga
     2281 tcgaacgcgc tgatcgtgtt gttcgtcgcg acaccggtca cggcgacggc gggcgtcacg
     2341 gcggcggtga cgatgaggcc cgcgatggcg ctcagacaga cgaagccgaa aagagcgccc
     2401 agagccccgg tgagggagcg tttgtcggcg ggcatgcaac ctagggtacg gggatttctc
     2461 ccgtcggcgg agaaccgaaa gaataagagg gggcgggcgg tgaaggcccc ggttgtgcag
     2521 gccgcctgag gcggagccgc cacccgccgg cacctaggcg gcgagtgacg gcgccagaag
     2581 gaagtggctg agaggtgtgc cgggctgcgt gacgcggccg gcgggcgctc tcaagagaga
     2641 gaggacggca gatggcatcg gcttcggaac gtttgaggga actcggaatc acgctgccgc
     2701 atgtgccggc gccggtcgcg gcatacgtgc cggcggtcgt cagcggtcag gtggtgcaca
     2761 cctccggcca gctgccgttc gtgggcggtg tgctgcccga agcgggcaag gtcggcgagg
     2821 gcgagggtct cgtctcgccc gaggcggcgc gcgggtacgc gcggcaggcg gcgctcaacg
     2881 cgcttgccgc ggtgcagtcg gtcatcgggt cgctcgaccg tgtcgtccgc gtggtgaagg
     2941 tcaccggatt cgtcgcctcg gccgccgggt tcgccggcca gtccacggtc gtcgacggcg
     3001 cctcgacgct ggtcggcgag atcttcgggg agcgcggccg gcacgcccgc tcgtcggtgg
     3061 gcgtggccga actgccgctc ggctcaccgg tcgaggtgga gttgagcgtc gaattcgcct
     3121 gaagggtcgg cgagcacggc gggcagccag ccgcgcagcg agctgacgac ggcgatcgcg
     3181 tcggcccggc gtaggtcggc gatgctcagg gagcgctcct gcacgatgcc gtcggcgatg
     3241 agcctgcccc gcccgacgcc gggcaggcag ccgtcggcga ccgggggcgt gaaccacagc
     3301 ccggccaggc gaacgacgat gttcgcgatg ctcgtttccg tgatgttccc gttcgtgttg
     3361 acgagcacgg tgtcgtcggc ctccgggtgg cgctctctgg ccgcggcgta gaccgcacga
     3421 agagtggtct tgtggcgcag ccagcgggat ctggggtcga tacggggcgt gtcgatcgcg
     3481 agggtgcgcg gccggctcgc ccttttcggc gcggcgccga ccgtgatcgt gcagcggccg
     3541 tgccggtcga gggcgatgcg caggcgccga tcgccgtgca ggccgcctct gccgtcgcgg
     3601 cggggagggt tgccggggtt gccggggtcg ccgggattgt cggggtcgtc agggttgccg
     3661 ggcgccgttg ccgccgccag gcgggcgcga atctcgtctt cttccagggg aaatccgaag
     3721 tagtgtgccg aggcgcgcag ccgggcgaga tgctccgtca ggtgcaccgg ccggccgtcg
     3781 tgctcccgca gcgtctcgat cagcgagaag tcttcttccg gcgggtcacg cagcactgcg
     3841 gtcttggcga ggatctcggc gaactcgcct tccgcactcg atccccaggt gatgccgccg
     3901 cccacgccgt attcggcccg gccggtggtg cggtcgacga cgaccgtgcg gatcgccacg
     3961 ttgaaccgcg cccgcaccgc gtggcccggc ggcgccagcc agccgatcgc gccgcagtag
     4021 aggccgcgcg ggctcgtctc gagctgcgcg atgagctcca tcgtgcgcgc cttgggggcg
     4081 ccggtcaccg agccgcaggg aaacagggcc gcgaggatcc cggcgaggct tgcctcccgc
     4141 tcgatccggg cggtgatggt cgaggtcagc tgccagagcg tcgcgtatct ctcgcaggct
     4201 aagagggccg gcacctgcac gctgccaggc gcggcgatcc gggagaggtc gttgcgcatg
     4261 aggtcgacga tcatcacgtt ctcggcgcga tctttggcgc tagcgagcag cgcgcgacgc
     4321 agaacctcgt cggaggccga atcgggcccg cgcgggctcg tgcccttcat cggcgtgcag
     4381 cggatctcgt cgccacgcca ttcgaaaaag ctctccgggc tggcgctcgc cacggcgaac
     4441 cggtcggtca ccagccacgc cccgttgtcg ccgccctggt tgcgcagaag gctgcggtag
     4501 tagccgaaca aggcctccgg cgtcgtgtcg caggcgaggc ccgtcatgcg ggcggagagg
     4561 ttgcattggt aggtttcgcc ggctgcgatc gcggcgcgca cggcgtcgaa gcgggtggtg
     4621 tattcggcgc gcgtggtgtc cagccgccag ccgccgaccg ccgcctgagc ggcgtgggcc
     4681 gcgccgcgtg tcgccggtgc ggtcgccgtt gttgacgtcg cggcggggcc gatgcgggtc
     4741 gggggatggt cggtgagtgc gaaccagacg aggggcgtcg gtgggtgtgg tcgagcgc
//
LOCUS       DASTNL010000173        10345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_314021, whole genome shotgun sequence.
ACCESSION   DASTNL010000173 DASTNL010000000
VERSION     DASTNL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314021"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFQ96_05355"
     CDS             <1..379
                     /locus_tag="VFQ96_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEU0257251.1"
                     /translation="VTRRPTFRGLVRSRRAVIPASGYYEWRHAGDTRAEPYYIHPAEG
                     LLYFAGLYSWWADPARPRDDPGRWHLTTTILTRAAVGDIRRLHDRAPVTLPDDLVAAW
                     VDPRTAGDAAFVAAAVDAATPVT"
     gene            564..2336
                     /locus_tag="VFQ96_05360"
     CDS             564..2336
                     /locus_tag="VFQ96_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU0257252.1"
                     /translation="MSAPPQAEVHILYGTESGDAEDLATDLARALAGQGVASTTTELD
                     NQPIDRLRSLGTALVVVSTYGEGDMPYDAEEFWDRLNEPDVPACTGLRYAVLALGDSS
                     YTYFCQAGVLIDERLAELGGTRLVDRVDCDVDYDERAQAWIAARAHQLRSLTPGGGVG
                     GGGPTADEGAVAAHPDGRPPETAAPLPPLRDDACGPELATTTPAAITQPAGAVREPAS
                     PSWDRHHPYPARLTRVVPLTGASSSKEVLHYELDLGQSGITVQPGDSLAVIAQNDPYA
                     VEHFLRAARLSGWWPGGGHQLRRLAREQWELHFPSAGLLAEIAQRAPYSPLGSLVVAG
                     DRAGVEAWTRRHSVADALEQLPAPIPRARLRELMGPLRYRAYSVSSSMLAHPGSAHLA
                     VTTKRLPKGRSMRSGVASGFLADRLPPGERVQVYPLPNRAFHLPDDDAPIVMIGPGVG
                     VAPFRAFLEERTLRSATGPSWLFFGDRNESTDFLYRDDWEGFAARGQLTRIDTAFSRD
                     TAGRRHTASQEHVQTRMREHGTELTRWLQDGAYLYVCGDARQMAGDVEKTLTAILADN
                     LGREAGAALFARLRAERRYRRDVY"
     gene            2430..3446
                     /locus_tag="VFQ96_05365"
     CDS             2430..3446
                     /locus_tag="VFQ96_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU0257253.1"
                     /translation="MAQSPATPTPYEILGVPATASQDDLRRAYRRMLRLSHPDMGGDA
                     ERFQAVQDAWQRVGDPARRAAYDRGSGPGSEAAGEPFSAGSSWPAGRPGNDGPTTSSS
                     GPHHSASWRARPGGRAGSPLAKTYGFPGARARERFLTLIEEWAGRGSHVEDPYDVSFV
                     RSAPPEIRQELAKAMAEEETARQLAALGIGFTVWNAVAGWDDDYQIDHVVLGPAGLFA
                     IHSADWGCRVALTRAEVTGDALAHKETPLADLAYNARKVAHGLGVRFTASIVVVPDDD
                     VDDIIDTVRRGRLAGSCLVARSALPGVLRGTIGVAADRGTVATADLMEVRTRLQQGLE
                     LVPV"
     gene            3519..4103
                     /locus_tag="VFQ96_05370"
     CDS             3519..4103
                     /locus_tag="VFQ96_05370"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEU0257254.1"
                     /translation="MRRRVSSAVRGLVMDWLYLIGVVFKGLDAILELAVGIPLLFISH
                     VQLLHIVQAVTAGELVEDPHDFLANLYLHEAARIGSAGMLVGAIYLVVHGGVKLPMVI
                     ALVRGSKRAYPWAVGVLSALLVVQIIDLALHYTTGVLLLSVLDAVIIALFLREWRHHR
                     PFMAVLHARASWLWRWRTCAIGFRSAVRGGWSSR"
     gene            complement(4133..4585)
                     /locus_tag="VFQ96_05375"
     CDS             complement(4133..4585)
                     /locus_tag="VFQ96_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257255.1"
                     /translation="MSCIRFNTPAQRQAMRDHAPARSAGRAAHPMPPVTESKIRRLRL
                     LAADKNPKIRESVASSRNAPQDLLARLAEDPDEGVRGCVARNETTSCDLLRALAGDRS
                     QTVRGWVAVNYFVPADVMATLAHDGSATVRGLVAWKDRLARQAASTGA"
     gene            4695..5165
                     /locus_tag="VFQ96_05380"
     CDS             4695..5165
                     /locus_tag="VFQ96_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0257256.1"
                     /translation="MSTPDGQSQPAQGRPSDAEVTARFGVEFRAALSALDGVVSAEEQ
                     TAVNALPAGSALLIVRRGQGIGSRFLLDSDTTSVGRHPHADIFLDDVTVSRRHAEFRR
                     EGDAFTVHDLGSLNGTYYNGVRIESATIADGAEVQIGKYRLTFYASRAGAAATE"
     gene            5169..5849
                     /locus_tag="VFQ96_05385"
     CDS             5169..5849
                     /locus_tag="VFQ96_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0257257.1"
                     /translation="MARQNAAAASLLGIGQVLAKLSPEFPDLTPSKLRFLEEQGLVSP
                     SRTRAGYRKFSSADVERLRLVLTMQRERYLPLRVIHRYLDDLDAGRAPRLPGAVGAAS
                     ILDSGQRLTKAALLARTGASAALFSEAVSASLISPAQTYDEGALRILTALVALWRSGI
                     EPRHLRTFRGAWEREVSLIESALLPVSRHPGSSSKERAAELAKDIARNLDVIRSGMVR
                     RGLNDRGF"
     gene            5978..6526
                     /locus_tag="VFQ96_05390"
     CDS             5978..6526
                     /locus_tag="VFQ96_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0257258.1"
                     /translation="MGELRIDGPRFDSGLLFTDGLPEIREAGGYRGAIAARAAGISYR
                     QLDYWARTGLVEPTVREATGSGSQRRYGFRDILVLKLVKRLLDTGISLQQIRTAVDQL
                     REAGVRDLAQTTLMSDGASVYLCTSDDEVIDLVGRGQGVFGIAVGKVLHEVESSLVEF
                     GTAPDEGDELAARRDAKRRRIS"
     gene            complement(6603..7409)
                     /locus_tag="VFQ96_05395"
     CDS             complement(6603..7409)
                     /locus_tag="VFQ96_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0257259.1"
                     /translation="MHVLSVSSLKGGVGKTTVTLGLASAAFARGLRTLVVDLDPQSDV
                     STGMDISVGGHLNVADVLASPKAKVVRAAIATSGWCRGRSSAIDVMIGSPSATNFDGP
                     HPSMADIWKLEEALAHVEDDYELTLIDCAPSLNALTRTAWAASDRVSVVTEPGLFSVA
                     AADRALRAIQEVRRKVSPRLQPLGIIVNRVRMRSVEHQFRIKELQNMFGPLVLSPQLP
                     ERTSLQQAQGAARPLHAWPGESAQEMAHNFDLVLDRVLRSARIGVAASVV"
     gene            7488..>10345
                     /locus_tag="VFQ96_05400"
     CDS             7488..>10345
                     /locus_tag="VFQ96_05400"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEU0257260.1"
                     /translation="MFRKILVANRGEIAIRAFRAAYELGAQTVAVFPYEDRNSLHRLK
                     ADEAYQIGEPGHPVRAYLDIDEIIRVAKESGADAIYPGYGFLSENPLLAKAAAEAGIT
                     FIGPGEHVLEMAGNKVTAKEHAREAGVPVLASTPPSKDIDELMAGAEEIGFPIFAKAV
                     AGGGGRGMRRVETLEELRPALESAMREADTAFGDATMFLEQAVLRPRHVEVQILADKT
                     GETVHLFERDCSVQRRHQKVVEIAPAPNLDDSIRESLHADAIRFAKSIGFVNAGTVEF
                     LVDTAGPRAGQHVFIEMNPRIQVEHTVSEEITDVDLVQSQMRVAAGETLADLGLHQDE
                     IQRHGAAIQCRVTTEDPTHDFRPDTGRITTYRSPGGAGVRLDGGTVNLGAQISPHFDS
                     MLAKLICRGRDFHSAIVRARRALAEFRIRGVTTNIPFLQAVLEDPSFEAGDLSTSFIE
                     ERPELFDGRVSKDRATKVLSWLADVTVNQPNGPRPTRTNLAAKLPDVDLSAPAPDGSR
                     QRLLRLGPAGFAADLRAQTALAVTETTFRDAHQSLLATRVRTKDIVRMAPYVARVTPQ
                     LLSVEAWGGATYDAALRFLNEDPWQRLAELRSALPNVNIQMLLRGRNTVGYTPYPTEV
                     TEAFVAEAAATGIDIFRIFDALNDVDQMQPAIDAVLATGTTVAEVAMCYTGDLMNPAE
                     DLYTLDYYLHLAERIVAAGAHILAIKDMAGLLRPYAAERLVTALRQRFDLPVHVHTHD
                     TAGGQLATLLAAAQAGADAVDVASAPMAGTTSQPSASALVAAFAHTERDTGIPLDAIC
                     SLEPYWEAVRALYRPFASGLTGPTGRVYHHEMPGGQLSNLRAQATALGLADNFELIED
                     LYAEADRMLGRVPKVTPSSKVVGDLALALAAAHADPADFEAHPEKYDVPESVIGFMAG
                     ELGDLPGGWPEPFRSKVLAGRDVSIAVPELDPEDARAL"
BASE COUNT         1621 a         3756 c         3451 g         1517 t
ORIGIN      
        1 cgtcacccgc cgcccgacct tccgcggcct cgtgcgcagc cggcgcgcgg tcatccccgc
       61 cagcggctac tacgagtggc gccacgccgg cgacacacgg gcggagccct attacattca
      121 cccggcagag ggcctgctct acttcgccgg gctgtactcc tggtgggcgg atcccgcgcg
      181 cccgcgggat gaccccggcc gctggcacct caccacgacg atcttgaccc gtgccgccgt
      241 cggcgacata cggcgcctcc acgaccgcgc acccgtcacc ctgcccgacg acctcgtggc
      301 cgcatgggtc gacccgcgca cggcgggcga cgccgcgttc gtggcggcgg cagtcgatgc
      361 cgccaccccg gtcacgtgag gcctgcgctt ccaccgcgtc gcccccgtga gaggcgactc
      421 cccgcacctc atcgaccccg cgtgatcccc gcgcgggagg ccgcgcgatc cccgtcaccg
      481 ggcatccgcg aagcccgatc ctctttttta cgaatacgtt ccggtaaaat cgatgtcgtg
      541 gcgaggatca ggccggacat ccgatgagtg cacccccgca ggcagaggtg cacatcttgt
      601 acggcacgga gtcgggcgac gccgaagatc tcgccaccga cctcgcccgg gcgttggcgg
      661 ggcagggcgt cgcctccacg accacggaac tcgacaacca gcccatcgac cggctccgtt
      721 cgctaggcac ggctctcgtc gtcgtctcca cctacggcga gggcgacatg ccttacgacg
      781 cggaagagtt ctgggaccga ctgaacgagc ccgacgtgcc ggcctgcacg gggctgcgct
      841 acgcggtcct cgctctcggc gacagctcgt acacatactt ctgccaggcc ggtgtgctta
      901 tcgacgaacg cctcgccgag ctcgggggga cgaggctggt cgaccgagtc gactgcgacg
      961 tggactacga cgagcgtgcg caggcctgga tcgcggcccg ggcccatcag ctgaggtccc
     1021 tcaccccggg cggtggcgtg ggtgggggcg ggccgaccgc cgatgaaggt gccgtcgcgg
     1081 cgcaccctga cggccggcca ccagagaccg ccgcaccgct gccccctcta agagacgacg
     1141 cctgcggccc ggagctcgcg acgacgacgc cggccgccat aacgcagccg gccggcgcag
     1201 tgcgggaacc ggcctccccc tcgtgggaca ggcaccatcc gtaccccgcc cgactgaccc
     1261 gcgtggtgcc gttgacgggt gcgagcagca gcaaagaggt gctgcactac gagctcgacc
     1321 tggggcagag cggcatcaca gtgcagcccg gtgattctct ggccgtcatc gctcagaacg
     1381 acccgtacgc ggtcgagcat ttcctccgcg ccgcgcgcct ttccggctgg tggccgggtg
     1441 gcgggcacca gctgcgccgt ctcgcgcgag aacagtggga actgcacttc ccctccgccg
     1501 ggctcctggc cgagattgcc cagcgggcac cctacagccc cctgggttcc ctcgtggtcg
     1561 ccggagaccg cgccggagtc gaggcctgga cgcgaaggca cagcgtcgcc gacgctctcg
     1621 aacaactccc ggcaccgatc ccgcgggccc ggcttcgcga actgatgggg ccgctgcgct
     1681 accgtgccta ctcggtgtcc tccagcatgc tcgcccaccc cggatcggcg cacctggccg
     1741 tcacgaccaa gcggctgccg aagggccgga gcatgcgctc cggggtggcc agcggattcc
     1801 tcgccgaccg cctgccgccc ggcgagaggg tgcaggtcta cccgctgccg aaccgtgcgt
     1861 tccacctgcc cgacgacgat gccccgatcg tcatgatcgg ccccggggtc ggcgtcgcgc
     1921 cgttccgcgc attcctggaa gagcgcaccc tgcgctcggc gaccggcccg tcatggctgt
     1981 tcttcggcga ccggaacgag tcgaccgact ttctctaccg agacgactgg gagggcttcg
     2041 ccgcccgcgg gcagctcacg aggatcgaca cggccttctc ccgcgacacc gccgggcgcc
     2101 gccacacggc ttcccaagaa cacgtgcaaa cccgcatgcg cgaacacgga acggagctca
     2161 cccgatggct tcaggacgga gcatacctgt acgtgtgcgg cgacgcgcgg cagatggccg
     2221 gcgacgtcga aaagacgctc accgcgatcc ttgccgacaa cctgggccgc gaggccggcg
     2281 cggccctctt cgcccgcctg cgcgccgagc ggcgctaccg gcgcgacgtg tactgacgac
     2341 cccgtcacag ggagccgtgg ggtcgcgacg gtaaactgtg cgccgtccga aggacgggcc
     2401 cttcggagtg gaacgcgaaa ggcaggcaca tggcgcagag cccggcgaca ccgaccccgt
     2461 acgagattct cggggtgccc gccactgcga gccaggatga cctgcgaagg gcctatcgcc
     2521 gcatgctccg cctctcccac cccgacatgg ggggagatgc ggagcgcttc caggccgtgc
     2581 aagacgcgtg gcagcgggtc ggcgacccgg ccaggcgggc cgcctacgac cgcggcagcg
     2641 ggccggggag cgaggcagcg ggcgagccct tctctgccgg gtcatcgtgg ccggccggcc
     2701 ggccggggaa cgacggcccg acgaccagct cctccgggcc gcaccacagc gcctcgtggc
     2761 gggcccggcc gggcggccgc gccggctcgc cgctcgcgaa gacctacggt ttcccgggcg
     2821 cccgcgcccg ggaacggttc ctgactctga tcgaggagtg ggccgggcgc ggcagccacg
     2881 tcgaagaccc gtacgacgtg tcgttcgtgc gctccgcccc gccggagatc cggcaggagt
     2941 tggcgaaggc catggcagag gaggagacgg cacgtcagct ggccgcactc ggcatcgggt
     3001 tcacggtgtg gaacgcggtc gccggctggg acgacgacta ccagatcgat cacgtcgtgc
     3061 tcggcccggc cgggctgttc gccatacatt cggccgactg gggctgccgt gtcgccctca
     3121 cccgcgccga ggtgaccggc gacgccctcg cacacaaaga gaccccgctg gccgacctcg
     3181 cctataacgc gcgcaaggtt gcccacggcc tcggcgtgcg tttcacggcc tcgatcgtgg
     3241 tcgttcccga cgacgacgtc gacgacatca tcgacacggt gcgccgcggc cggctcgccg
     3301 gctcgtgcct cgtcgcccgg tcagccctgc ccggcgtgct gcgcggcacg atcggcgtcg
     3361 cggccgaccg tggcacggtt gccaccgccg acctcatgga ggtgcgtacc cgcctgcagc
     3421 agggcctgga gctggttccg gtctgaccgc cgcagcgccc gaagcgggcc ggcggcatgc
     3481 tgagaggatt ctttgaccgg tgccgtcaga ctcacagcat gcgtcgacgg gtgtcatcgg
     3541 ccgtacgcgg cctggtgatg gactggctgt acctgatcgg tgtggtgttc aaggggctgg
     3601 acgccattct ggagctggcc gtaggcatcc ccctgctgtt catcagccac gtgcagctcc
     3661 tgcatatcgt gcaggcggtc acggcggggg agctggtcga agacccgcac gatttcctcg
     3721 cgaacctgta tctgcacgag gctgcccgca tcggctctgc cgggatgctc gtgggtgcga
     3781 tctatctcgt cgtgcacggc ggggtgaaac tgccgatggt catcgcgctc gtccgcgggt
     3841 cgaaacgcgc ctacccctgg gccgtcggcg tgctgtctgc tctgttggtc gtgcagatca
     3901 tcgacctcgc cttgcactac acgaccgggg tactgctgtt gagcgtgctg gacgccgtga
     3961 tcatcgcgtt gttcctgcgc gagtggcgac atcatcggcc gttcatggcc gtgttgcacg
     4021 cccgggcatc ctggctgtgg cgctggcgta cctgcgctat cgggttccgg tcggccgtac
     4081 gcggcggctg gtcgagccgg tgaccgtcga gtcggtgacc caaccggtca cgctatgcgc
     4141 cggtcgaggc ggcctgcctg gccagccgat ccttccacgc gacgaggccg cgcaccgtcg
     4201 cgctcccatc gtgcgccagg gtcgccatga cgtcggccgg cacgaaatag ttgacggcga
     4261 cccaaccgcg caccgtctgc gagcggtcac cggcgagggc gcgcagaagg tcgcacgagg
     4321 tcgtctcgtt gcgtgcgaca cagccacgca ccccctcgtc ggggtcttcg gcgagcctgg
     4381 ccaggaggtc ttgtggggcg ttacgactgc tggccaccga ttcgcggatc ttcggattct
     4441 tgtcggccgc cagaagccgc aaccggcgga tcttgctctc ggtgaccggc ggcatcggat
     4501 gcgccgcccg gcccgccgac cgggcgggcg catgatcgcg catcgcctga cgttgggcag
     4561 gggtgttgaa ccggatgcac gacatgttcc gacgctaccg cgtgccaccg acgtccgccc
     4621 gacgaacggc ctggggagag tgggcgcggc gcggggagga tcggataatc ttggaggaca
     4681 aggaggtcca ggacgtgagc acacccgacg ggcagtccca acccgcgcaa gggcgcccga
     4741 gcgatgccga ggtcacggcg cgtttcggcg tcgagttccg ggccgcactg agcgcactgg
     4801 acggcgtcgt cagcgcggag gagcagacgg cggtcaacgc gctgccggca ggctccgccc
     4861 tgctcatcgt gcggcgcggc cagggtatcg ggtcgcgctt cctcctcgat tccgacacga
     4921 cgtcggtcgg ccggcatccg cacgccgaca tcttcctcga cgacgtgacg gtctcgcgcc
     4981 gacacgccga gttccggcgc gaaggcgacg cgttcaccgt tcacgatctc gggtccttga
     5041 acggcaccta ttacaacggg gtacgcatcg agagcgccac gatcgccgac ggcgccgagg
     5101 tgcagatcgg caagtatcgc ctcacgttct atgcgtcgag ggccggcgca gccgcgacgg
     5161 aatagccggt ggcacgtcag aacgccgcgg cggccagcct gctgggcatc ggccaggtgc
     5221 tcgccaagct ctccccggag ttccccgacc tcacaccgtc gaagctgcgt ttcctcgaag
     5281 aacaaggcct cgtctcgccg tctcgcacga gggccgggta tcgcaagttc tcgtctgccg
     5341 acgtcgagag actccggctg gtgctgacga tgcagcgcga gcgctacctg ccgttgcggg
     5401 tcatccaccg ctacctcgac gaccttgacg cgggccgcgc cccgaggctt ccgggcgcgg
     5461 tcggcgccgc atcgatcctc gactccgggc agcgcctcac caaggcggcc ctcctcgccc
     5521 gtaccggggc ttcggccgcc cttttcagcg aggccgtctc tgcctcgctg atttcccccg
     5581 cccagaccta cgacgagggg gcgctgcgca tcttgaccgc cttggtggcg ttgtggcgaa
     5641 gcggcatcga gccgcggcac ctgcgcacct tccgaggcgc ctgggaacgg gaggtcagtc
     5701 tcatcgaaag cgccctcctg ccggtctcac gccaccccgg ctcctcgagt aaagagcgcg
     5761 ccgccgagct tgcgaaagac atcgcccgca acctcgacgt gatccgctcc ggcatggttc
     5821 gtcgagggct gaacgatcgg ggcttctgag gtgtgcagcc gcaacacgcc gggcgctcac
     5881 gcccgatatc gttgccgcgc cggccgggca ccggtagcgt ggccctggcg acgttttcgc
     5941 gtcggtgtca cgggcgcgac gagaggcgaa ggcatacatg ggtgagctca ggatcgacgg
     6001 cccacgcttc gactccggcc tgctgttcac cgacgggctc cccgagatcc gcgaggcggg
     6061 cggctaccgc ggcgcgatcg ccgcgcgggc ggcgggcatc agctaccgtc agctcgacta
     6121 ttgggcgcgc accggcctcg tggagccgac cgtgcgagag gccacgggca gcggctcgca
     6181 acgccggtac gggttccgcg acatcctcgt cttgaagctc gtgaagcgcc tgctcgacac
     6241 cggcatctcg ctgcaacaga tccgcacggc ggtcgaccag ctgcgggagg ccggcgtgcg
     6301 cgatctcgcc cagaccaccc ttatgagcga cggcgccagc gtgtacctgt gcacctccga
     6361 cgacgaggtc atcgacctcg tcgggcgcgg gcaaggtgtg tttggcatcg cggtgggcaa
     6421 ggttctgcac gaggtcgagt cgagcctcgt cgagttcggc acggcccccg acgagggcga
     6481 cgagctcgcc gccaggcgcg acgccaaacg tcgccgcatc tcctagcctg cggcccggca
     6541 ccacgcaccc gacgatcgaa caggtgcgcg tggcccggcg gccacgtcat atccggttca
     6601 ggtcagacga cgctcgcggc cacgccgatc cgcgccgaac gcagcaccct gtcgagcacc
     6661 aggtcgaagt tgtgcgccat ctcttgcgcg ctctcacccg gccaggcgtg caacggccgg
     6721 gcggcaccct gcgcctgctg caacgacgtg cgctccggca gctgggggct gagaaccagc
     6781 gggccgaaca tgttctgcag ctccttgatg cggaactggt gttccaccga acgcatccgc
     6841 acccggttca cgatgatccc cagcggctgc agcctggggg agaccttgcg gcgcacctct
     6901 tgaatggccc gcagcgcccg gtcggcggcc gcgacagaga agaggcccgg ctcggtgacg
     6961 acgctcaccc ggtcgctggc cgcccacgcc gtgcgggtca gggcgttcaa cgacggcgcg
     7021 cagtcgatca gcgtcagctc gtaatcgtct tcgacgtgag ccaacgcctc ctcaagcttc
     7081 cagatgtcgg ccatcgacgg gtgggggccg tcgaaattcg tcgcggaggg gctgccgatc
     7141 atcacgtcga tcgcggagct gcgcccgcgg caccagccgc tcgtggcaat cgcagcccgc
     7201 accaccttcg ccttcggcga ggcgagcacg tcggcgacgt tcagatggcc gccgacggag
     7261 atatccatgc ccgtcgacac atccgactgc ggatcgagat cgacgaccag cgtgcgaagg
     7321 ccccgcgcaa acgcggcgga tgcgagcccc agagtcactg tcgtcttgcc gacgccgcct
     7381 ttgagcgagc tgacactgag tacgtgcaca agagccaaga ttaccttcac tagtcttaga
     7441 gaacctaacc atttgctgag cgcgtcagcg cagcagaaag gtccttaatg tttcgaaaga
     7501 tcctcgtcgc caaccgtggt gagatcgcca tccgtgcctt ccgcgccgcc tatgagctcg
     7561 gcgcgcagac cgtcgcagta ttcccttacg aagatcggaa ttcgctccac cgtctgaaag
     7621 cagacgaggc gtatcagatc ggcgagccgg gccaccccgt gcgcgcctac ctcgatatcg
     7681 acgagatcat ccgagtggcc aaggagtccg gcgctgacgc gatctacccg ggttacggct
     7741 tcctctccga gaaccctctc ctcgccaagg cggccgccga ggccggcata accttcatcg
     7801 gccccggtga gcacgtcttg gagatggccg gaaacaaggt gacggcgaag gagcatgccc
     7861 gagaggcggg cgtgcccgtg ctcgcctcca caccgccctc gaaagacatc gacgagctca
     7921 tggccggtgc cgaagagatc ggcttcccga tcttcgcgaa ggcggtcgcc ggcggaggcg
     7981 ggcgcggcat gcgtcgcgtc gagacgctcg aggagctgcg tccggccctc gagtcggcga
     8041 tgcgggaggc agacacggcg ttcggcgacg cgacgatgtt cctcgaacag gccgtgctac
     8101 ggccccgcca cgtcgaggtc cagatcctcg ccgacaagac cggcgagacg gtgcacctct
     8161 tcgagcggga ctgctcggtg cagcgtcgcc accagaaggt cgtcgagatc gccccggcac
     8221 cgaacctcga cgactccatc cgcgagagcc tgcacgcgga cgcgatacgg ttcgcgaagt
     8281 cgatcggctt cgtcaacgcg ggaaccgttg aattcctcgt cgacacggca gggccccggg
     8341 ccggccaaca cgtcttcatc gagatgaacc cgagaatcca ggtcgagcac accgtctccg
     8401 aagagatcac cgacgtcgac ctcgtccagt cgcagatgag ggtcgcggcc ggcgagacac
     8461 tcgccgacct gggcctgcac caagacgaga tccagcggca cggcgcggcc atccaatgcc
     8521 gtgtcacgac ggaggacccg acccacgact tccggcccga caccggccgg atcaccacgt
     8581 accgctcccc gggcggcgcg ggcgtgcgtc tcgacggcgg cacggtgaat ctgggcgccc
     8641 agatcagccc gcacttcgac tcgatgctcg ccaagctcat ctgccgcggc cgcgacttcc
     8701 actccgcgat cgtgcgggcc aggcgggcgt tggccgagtt ccgcatccgc ggcgtcacca
     8761 cgaacatccc gttcctgcag gccgtgctcg aagacccgag cttcgaggcg ggcgacctca
     8821 gcacctcgtt catcgaggaa cggccggagc tcttcgacgg acgggtctcg aaagaccgcg
     8881 ccaccaaggt gctcagctgg ctggccgacg tgaccgtcaa ccagcccaac ggcccgcgcc
     8941 cgacacgcac caacctcgca gcgaaactgc ccgatgtcga cctttcggcg ccggcgccgg
     9001 acggcagccg gcaacggctc ctgcgcctcg gcccggccgg attcgccgca gacctgcgcg
     9061 cccagaccgc gctcgccgtc accgagacga cgttccgcga cgcccatcag tcgctcctgg
     9121 ccacccgggt ccgcaccaaa gacatcgtgc gcatggcgcc ctacgtggcg cgggtgacgc
     9181 cgcagcttct ttcggtggag gcgtggggcg gggcgacgta cgacgccgcg ctgcgcttct
     9241 tgaacgaaga cccgtggcag cggctggccg aactgcgaag cgccctgccc aacgtgaaca
     9301 tccagatgct tctgcgcggc cgcaacaccg tcggctacac cccgtacccc accgaggtga
     9361 cggaggcctt cgtggcagag gccgcggcca ccggcatcga catcttccgc atcttcgacg
     9421 ctttgaacga cgtcgaccag atgcaaccgg cgatcgacgc ggtgctcgcc accggcacga
     9481 ccgtcgccga ggtcgcgatg tgctacacgg gtgatctcat gaacccagcc gaagacctct
     9541 acacgctcga ctactacctg cacctggccg aacgcatcgt cgcggccggc gcacacatcc
     9601 tcgccattaa agacatggcc ggcctcctgc gcccctacgc cgccgagcgg ctcgtcacgg
     9661 cgctgaggca acgcttcgac ctgccggtgc acgtgcacac gcacgacacc gccggcggcc
     9721 agctggcgac cctcctcgcc gccgcccagg ccggcgcaga cgcggtcgac gtggccagcg
     9781 cccccatggc cggcacgacg agccagccct cggcctcggc gctcgtggcc gcgttcgcac
     9841 acaccgaacg cgatacagga atcccgctgg acgcgatctg cagcctcgag ccgtattggg
     9901 aggcggtgcg cgccctctac cggccgtttg cctccgggtt gaccgggccg accgggcggg
     9961 tttaccacca cgagatgccg ggcgggcagc tctcgaacct gcgcgcgcag gcgacggcgc
    10021 tcggcctggc cgacaacttc gagctcatcg aagacctgta cgccgaggcc gaccggatgc
    10081 tgggccgcgt gcccaaggtg acgccgtcgt ccaaggtcgt cggcgacctc gcgctcgcgc
    10141 tcgcggcggc ccacgctgac ccggcagact tcgaagccca cccagagaaa tacgacgtgc
    10201 ccgaatcggt catcggcttc atggccggcg agctcggcga cctgccgggc gggtggccgg
    10261 agccgttccg cagcaaggtc ctcgccggcc gcgacgtctc gatcgcggtt cccgagctcg
    10321 acccggagga cgcccgcgcg ctcga
//
LOCUS       DASTNL010000174         7257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_500593, whole genome shotgun sequence.
ACCESSION   DASTNL010000174 DASTNL010000000
VERSION     DASTNL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500593"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..519
                     /locus_tag="VFQ96_05405"
     CDS             280..519
                     /locus_tag="VFQ96_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEU0257261.1"
                     /translation="MRRCSRTACTAEAVATLTFDYADSMAVLGPLALVREPHSYDLCA
                     RHAERLRAPRGWQIVRYRSQASGEGDEAGVARGGA"
     gene            532..2040
                     /gene="ahcY"
                     /locus_tag="VFQ96_05410"
     CDS             532..2040
                     /gene="ahcY"
                     /locus_tag="VFQ96_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU0257262.1"
                     /translation="MDHPASKRQAGAARPAVFDYRVADLSLAAAGRHQLRLAENEMPG
                     LMALRREFGATKPLAGARIAGSLHMTVQTAVLIETLVALGARVRWASCNIFSTQDEAA
                     AAVVVGPTGTPEDPAGVPVFAWKGESVEDYWHLTARIFDWSAENRDLHEAGGTPEGPG
                     PDLILDDGGDATLFVHAGVWAENGEEPAGESREERALVAVLREVLDAEPGRFTRIAGH
                     LLGASEETTTGVHRLHKLLREGRLLFPAIDVNDAVTKSKFDNRYGIRHSLPDGLNRAT
                     DVLIGGKVAFVVGYGDVGKGAAEALSGQGARVIVGEVDPITALQAAMDGYQVARLDDV
                     VGLVDMVVTCTGNYHVVTTAQMLAMKHLAILANVGHFDNEIDMAGLEALEGATRVPIK
                     PQVDEWRLPNGRSILVLSEGRLMNLGNATGHPSFVMSASFTNQVLAQIELYTHREAYP
                     LGVHMLPKLLDEKVARLHLGALGARLTTLTPEQADYIGVGVEGPYKPDTYRY"
     gene            complement(2034..3026)
                     /locus_tag="VFQ96_05415"
     CDS             complement(2034..3026)
                     /locus_tag="VFQ96_05415"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU0257263.1"
                     /translation="MAALPPQDARPAPARGRDPGPSDARGDTEAVVVGEAVGLSVRPA
                     GLLARAAGTIIDWAVYGGVFVGLALAVHAFSGGLDQAAVRALSIAALVLALVAVPTAV
                     EVASAGRSAGRAALGLRIVRSDGGAASVRHAFIRALVGVVEIFVSFGSIAMLVALLDP
                     RARRIGDFLAGTYSQLERVPKPPALKHRVPPELASWSSIADVAPLPGRLAGRIAAFLS
                     QQGGMLPAARDRLARSLLDEAADYVWPLPKAPAAEALAAIAAVRAERDRRLLAREDAI
                     VAGLRPQLTGTAHHPSHGLPAGPRGQETGRPADGVATVPTLDAKPPGPGHAPRQ"
     gene            3044..4039
                     /locus_tag="VFQ96_05420"
     CDS             3044..4039
                     /locus_tag="VFQ96_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU0257264.1"
                     /translation="MDLDVLRESRQGGWDRLDALARRRIASGEAADELIERYRAGAAD
                     LATIAGQSAASPLGERLSVSLARARLRFTGAGPDLFSRVPTFFVRQLPAALYRLRWLT
                     AVIAAATALIAAAFALWANVDPRVMAAIGDPAQLKDLAENAFTGYYSQSPAAAFAGQV
                     WTNNALIAALCIVCGITGVYPPYVIVQNAMNIGVDGAVLFSYGRGGVFFQYIAPHGML
                     ELTSVFVAAAAGLRIFWAWVAPGARSRAQALAEDGRALVTVSVGLTLSLLVSGLIEGF
                     VTPSALPWPVKVGIGALALAAFLCYMLVLGRRAAEAGDTGDVAEFDAGARRLVAG"
     gene            complement(4036..5385)
                     /locus_tag="VFQ96_05425"
     CDS             complement(4036..5385)
                     /locus_tag="VFQ96_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0257265.1"
                     /translation="MVVTGRFVLLLALGTIPTIAAGRAGAAWASAGLWLAACLGMAAA
                     DAALAATPRRVALRRDLPARARLGEPVAGVVVVGNLGRRRMRALLRDRWQPTAGVEPA
                     RRRVALAAGESVGVPFLLRPRRRGLVRSAGVTLRTMGPLGIAGRQASLTAPGLLKVLP
                     PFRSRRHLPSRLRMLRQEGGRQALQVRGAGTEFDSLRQYVRGDDVRSIDWRATARRGP
                     AGPGTGRAGATAAHEPMVRTWRPERDRQVVIVVDSSRLAAARIGDETRLDTSVEAALL
                     LAVLADRAGDRVGLVVFDRRVRARVSGIRGTALPAAIVDRVAGVWPELVEADWAALAA
                     EAQSLTRGRCLVVLLTTLDAPASARGLLTALPGLTARHTVVVAGALDPAIREATTRRR
                     DRAEIYRAAAAERAVLDSARVAAAARRLGATVVAAPADDLPAAVADRYIALKVRGLI"
     gene            complement(5388..6434)
                     /locus_tag="VFQ96_05430"
     CDS             complement(5388..6434)
                     /locus_tag="VFQ96_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0257266.1"
                     /translation="MSDDTPHSAGPATPGRVDAENRTATDGGGTAADAATRQALRAVR
                     DEVARAVVGQEGAVTGLIIALLVRGHVLLEGVPGVGKTLLVRSLGRALDVATTRIQFT
                     PDLMPGDVTGSLVYDTSTGSFVFREGPVFTNILVADEINRTPPKTQSALLEAMEEHQV
                     SADGVTRPLPDPFLVAATQNPIEFEGTYALPEAQLDRFLFKLMLDPPPRDEEVAVLRR
                     HADGFDPHDLAAAGVRPVLDAAGLRRAREAVGALPFDTDLLGYVVDLARATRRSPSVQ
                     IGVSPRGATALAAAARAWAWLSGAPAVTPDHVQAMLLPAWRHRVRLRAEAELEGVEVD
                     ALLSSLVQQTQVPL"
     gene            complement(6431..>7257)
                     /locus_tag="VFQ96_05435"
     CDS             complement(6431..>7257)
                     /locus_tag="VFQ96_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257267.1"
                     /translation="GAIRPTTPLTAHCTGDATTSARRILVEGSSYRAGDGLRGGCFPT
                     ADGYAMLSLPSPRHTTIVGSAELFENGSIGRADNAAAAIGVAGETPTLVWYLPTVADV
                     PAASRPTLAALTPGWVTPTALLLIATALAAIAWRGRRFGPLVTEALPVTARSNETLEG
                     RARLYRLASARGHALESLRIGTLGRLAARLGLPETASAAEVADAAAAAAGQDPGRVRA
                     TLIGEAPGGDAHLVRLSERLASLEARVAAALRPEAFSANAAPPPHTEPTQPTEGNA"
BASE COUNT          983 a         2606 c         2703 g          965 t
ORIGIN      
        1 gatggcctcg gcaggtccct gtgcgacttg gaacgtgacg ccctcgagtt cgggccagag
       61 gccgcgcagg tacgcggccg tcgaggccac cgtcatgtcg aagtcgtcga cacgcgtgcg
      121 aaggggctcg aggtgcggtc cggtcacggc cgaccgcatc ccacgcccat ggcgatctcg
      181 gatgccgcgt ctcggcgacg gtggaaccgc ggcgacacga cgggaccggg gcatgccctc
      241 catcgtaccg ggcccgcgga tcgctacgct ggtgtcgcca tgagacgttg ttcgcgcacc
      301 gcatgcaccg ctgaggcggt cgcgacgctc acgttcgact acgccgactc catggcggtg
      361 ctcggaccgc tggccctcgt gcgagagccg cattcgtacg acctgtgcgc ccgccacgcc
      421 gagcggttgc gcgcgccgcg cgggtggcag atcgtgcggt accggtcgca ggcctcgggc
      481 gagggcgacg aggcgggcgt ggcgcggggc ggggcctgag acaataccgg catggatcat
      541 cccgcttcca agcgccaggc cggcgccgca cgtccggctg tgttcgacta ccgggtcgcc
      601 gacctttcgc tcgccgcggc cggccggcat cagctgcggc tggcagagaa cgagatgccc
      661 ggcctcatgg cgctacgtcg ggagttcggt gcgacgaagc cgctggccgg tgcccgcatc
      721 gccggctcct tgcacatgac ggtgcagacc gccgtgctga tcgagaccct cgtcgcgctc
      781 ggcgcccggg tgcgctgggc cagttgcaac atcttctcca cgcaagacga ggcggccgcc
      841 gcggtcgtcg tcggcccgac gggcacgccg gaggatccgg caggggtgcc cgtcttcgct
      901 tggaagggcg agagcgtcga agactattgg cacctgaccg cgcggatctt cgactggagc
      961 gccgagaacc gcgacttgca cgaggccggc ggcacgcccg aggggcccgg ccccgacctc
     1021 attctcgacg acggcggcga cgccacgctc ttcgtccatg ccggggtctg ggccgagaac
     1081 ggcgaggagc cggccggcga gagccgtgag gagcgggcgc tcgtcgccgt gttgcgtgag
     1141 gtgctcgatg ccgagccggg ccgcttcacg cgcatcgccg ggcacctctt gggggcgagc
     1201 gaagagacga cgacgggcgt gcaccggctg cacaagctgc ttcgcgaggg gaggctgctc
     1261 ttccccgcca tcgacgtcaa cgacgcggtg acgaagagca agttcgacaa ccggtacgga
     1321 atccgccatt ccctgcccga cgggttgaac cgcgccaccg acgtgctcat cggcggcaag
     1381 gtcgccttcg tcgtcggcta cggcgacgtc ggcaaggggg ccgcggaggc gctgtccggc
     1441 caaggcgcgc gtgtcatcgt cggcgaggtc gacccgatca ccgcgctgca ggccgcgatg
     1501 gacgggtatc aggtggcccg gctcgacgat gtcgtcggcc tcgtcgacat ggtcgtcacc
     1561 tgcaccggca actaccacgt cgtgaccacc gcgcagatgc tcgccatgaa gcacctcgcg
     1621 attcttgcca atgtcggcca cttcgacaac gagatcgaca tggccggcct cgaggccctc
     1681 gagggcgcga cccgggtgcc gatcaagccg caggtcgacg agtggcggct gccgaacggg
     1741 cgtagcatcc tcgtcttgag cgaggggcgg ctcatgaacc tcggcaacgc gaccggtcac
     1801 ccctccttcg tgatgagcgc gtcgttcacc aaccaggttc tggcccagat cgagttgtac
     1861 acccaccgcg aggcctaccc gctgggcgtg catatgttgc cgaaactgct cgacgagaag
     1921 gtcgcccgcc tgcacctggg cgcgctcggg gccaggctga cgactctcac acccgagcag
     1981 gccgactata tcggcgtggg cgtcgaaggc ccctataagc ccgacacgta ccgttactga
     2041 cggggggcgt ggccgggccc cgggggcttg gcgtcgaggg tcggaaccgt ggcgaccccg
     2101 tcggccgggc gccccgtctc ttgcccgcgt ggcccggccg gcagcccgtg agaggggtgg
     2161 tgcgcggtgc cggtgagttg ggggcgaagg cccgccacga tcgcgtcttc acgggccagg
     2221 aggcgcctgt cgcgctcggc gcgcaccgcg gcgatcgcgg cgagcgcctc ggcggcgggc
     2281 gccttgggca acggccatac gtagtcggcg gcctcgtcga gaaggcttcg ggccaaccgg
     2341 tcgcgggcgg ccggcagcat gccgccctgc tgactgagga acgctgcgat gcgcccggcg
     2401 aggcggcccg gcagcggcgc cacgtcggcg atcgacgacc acgaggcgag ttccgggggc
     2461 acgcggtgct tgagggccgg cggcttcggc acccgctcca gctggctgta cgttccggct
     2521 aggaaatcgc cgatccgtcg ggcgcggggg tccagcaagg cgacgagcat cgcgatgctg
     2581 ccgaacgaga cgaagatctc gacgacgccg acgagcgcgc ggatgaaggc gtgccgcacc
     2641 gaggcggccc cgccgtccga gcgcacgatg cgcaacccga gggcggcacg gcccgccgag
     2701 cggccggcgc tcgccacctc gaccgccgtc ggcacggcga ccagggcgag gacgagggcg
     2761 gcgatcgaca gcgcccgcac ggccgcctgg tcgagcccgc ccgagaacgc gtgaacggcc
     2821 aacgccaggc ccacgaagac cccgccgtag acggcccagt cgatgatcgt gccggccgcc
     2881 cgggccagaa ggccggcggg gcgcaccgac aggccgaccg cctcgcccac gacgacggcc
     2941 tccgtgtcgc ctcgcgcgtc cgacgggccc ggatcgcggc cgcgggcggg cgcaggccgg
     3001 gcatcctggg gaggcaaggc ggccacggtt atcatctaag cagatggatc tcgacgtgct
     3061 ccgggagtcc cgccagggcg gttgggacag gctcgacgcg ctcgcccgac gccgcatcgc
     3121 gagcggcgag gcggcggacg agctcatcga gcgctaccgg gccggggcgg ccgacctggc
     3181 gacgatcgcc ggccagtcgg ccgcctctcc gctgggtgaa cggctctccg tgagcctcgc
     3241 ccgcgcccgc ctgcgcttca cgggcgccgg ccccgatctc ttctcccgcg tgccgacgtt
     3301 cttcgtccgc cagctgcccg cggcgctgta tcggctgcgc tggctgaccg ccgtgatcgc
     3361 cgcggccacc gcgctcatcg ccgccgcctt cgccctgtgg gcgaacgtcg acccgcgagt
     3421 gatggcggcg atcggcgacc cggcccagtt gaaagacctc gccgagaacg ccttcaccgg
     3481 ctattactca caaagcccgg ccgccgcatt cgccggccag gtgtggacga acaacgccct
     3541 gatcgcggcc ctctgcatcg tatgcggcat aaccggcgtg tacccgccct acgtgatcgt
     3601 gcagaacgcg atgaatatcg gcgtcgacgg cgccgtgttg ttctcctacg gccgaggcgg
     3661 ggtgttcttc caatacatcg cgccgcacgg catgctggag ctcacctcgg tgttcgtcgc
     3721 ggcggcggca gggctccgca tcttctgggc gtgggtggcg ccgggggcgc gcagccgtgc
     3781 gcaggcgctc gctgaagacg gccgtgccct cgtcaccgtc tcggtgggcc tcaccctctc
     3841 gctcctcgtc tccggcctca tcgagggctt cgtcaccccg tcggcgctgc cgtggcccgt
     3901 caaagtcggc atcggggcgc tggccttggc cgcgttcctc tgttacatgc tggtcctcgg
     3961 ccggcgtgcc gccgaggccg gcgacacggg tgacgtggcg gagttcgacg cgggggcacg
     4021 ccgtctcgtc gccggctaga tgaggccccg tactttcaag gcgatgtacc ggtcggccac
     4081 cgcggcgggc aggtcgtcgg ccggggcggc gaccacggtg gccccgagcc ggcgtgccgc
     4141 ggcggccacg cgggcggagt cgagcacggc gcgttccgcg gcggccgcgc gatagatctc
     4201 cgcccggtcg cggcgtcgtg tcgtggcctc ccggatcgcg gggtcgagcg ctcccgccac
     4261 gacgacggtg tgccgggccg tgagcccggg cagcgccgtg aggaggcctc gggcggaggc
     4321 gggggcgtcg agcgtcgtca gaagcacgac gaggcaacgc ccccgagtaa ggctctgggc
     4381 ctccgccgcc agcgcggccc aatccgcctc gacgagttct ggccacaccc cggcgacgcg
     4441 gtcgacgatc gccgccggca acgcggttcc ccggatgccg gagacccggg cgcgcacccg
     4501 gcggtcgaag acgacgaggc cgacccgatc gcccgcgcgg tctgccagca ccgcgagaag
     4561 cagcgcggcc tccaccgacg tgtcgaggcg cgtctcgtcg ccgatccggg cggcggcgag
     4621 acgggagctg tcgacgacga tgaccacctg ccggtcgcgt tccggtcgcc aggtgcgcac
     4681 catgggctcg tgggccgctg ttgcgccggc ccgccccgtg cccggtcccg ccggcccgcg
     4741 ccgtgccgtg gcccgccagt cgatggatcg cacgtcgtcg ccgcgcacgt attgtcgtaa
     4801 cgagtcgaat tcggttcccg cgccgcgcac ctgcaacgcc tgccggccgc cctcctggcg
     4861 cagcatgcgc agcctcgacg gcaggtgccg tcggctgcgg aacggcggca gcactttcag
     4921 caggccgggc gccgtgaggc tcgcctgtcg gccggcgatg ccgagcgggc ccatcgtgcg
     4981 cagcgtcacg ccggcgctgc gcacgaggcc tcgccgccgg ggtctcaaga ggaacggcac
     5041 gcccaccgac tctcccgcgg cgagcgcgac ccgccgccgg gccggttcta cgcccgcggt
     5101 gggctgccaa cggtcacgca ggagcgctcg catgcgtcgc cggccgaggt tgcccaccac
     5161 gacgacgccc gccacgggct cgccgagccg cgcccgggcc ggcaggtcac ggcgaagagc
     5221 cacgcgccga ggcgttgcgg cgagcgccgc gtccgcggcc gccatgccga ggcatgcggc
     5281 gagccagagc ccggcgctcg cccaggcggc gcccgcccgc ccggcggcga tcgtcgggat
     5341 cgtgccgaga gcgaggagca gaacgaacct gccggtgacg accatcgtta cagcggcacc
     5401 tgggtctgct gcacgaggga ggagagcaga gcatccacct cgacgccttc gagctctgcc
     5461 tcggcgcgta accgcacccg atgccgccag gccggcagca gcatcgcctg cacgtggtcg
     5521 ggggtcacgg cgggggcgcc ggagagccac gcccatgccc gcgccgcggc cgcgagggcg
     5581 gtcgcaccgc gtgggctgac gccgatctgc accgacggcg aacgcctcgt cgcccgggcg
     5641 agatcgacca cataccccag aaggtcggtg tcgaacggca acgcaccgac ggcctcgcgt
     5701 gcgcgccgca ggccggctgc gtcgagcacc ggccgcacgc ccgcggccgc gagatcgtgc
     5761 gggtcgaatc cgtcggcgtg gcggcgcagc acggccacct cctcgtcgcg gggcggcggg
     5821 tcgagcatca gtttgaagag gaaccggtcg agctgcgcct cgggcaaggc gtacgtgccc
     5881 tcgaattcga tggggttctg ggtcgccgcg acgaggaacg ggtcgggcag cggccgggtc
     5941 acgccgtcgg cggagacctg gtgctcctcc atcgcctcga gcagggccga ctgggtcttc
     6001 ggcggagtgc ggttgatttc gtcggcgacg aggatgttcg tgaacacggg gccttctcgg
     6061 aacacgaagg aaccggtcga cgtgtcgtag acgagcgagc cggtgacgtc gcccggcatc
     6121 aggtcggggg tgaattggat ccgcgtcgtc gccacgtcga gggcacgccc gagcgagcgc
     6181 acgaggagcg tcttgccgac gccgggcacc ccctccagaa gcacgtggcc gcgcacgagg
     6241 agggcgatga tgaggcccgt gacggcgccc tcctggccga cgaccgctcg ggcgacctcg
     6301 tcgcgcaccg cgcgaagcgc ctgcctggtg gccgcgtcgg cggcagtgcc gcccccgtcg
     6361 gtcgcggtgc ggttttcggc gtcgacgcgc cccggcgtcg ccggcccggc actgtgtggg
     6421 gtgtcgtcgc tcatgcgttc ccttccgtag gctgtgtcgg ttcggtgtgg ggcggcgggg
     6481 cggcgttcgc cgagaaggcc tccggtcgaa gcgccgccgc cacccgtgcc tccaacgatg
     6541 ccaggcgttc ggagagccga acgagatgcg cgtcgccgcc cggtgcctcg ccgataagcg
     6601 tcgcacgcac acggcccggg tcttgccccg cggccgcagc cgcggcgtcg gcgacctcgg
     6661 cggcgctcgc cgtctcgggc aggccgagcc gggccgcgag ccgaccgagt gtgccgatgc
     6721 gcaggctctc gagtgcgtgc cctctggcgc ttgccaggcg gtacaggcgg gcacgcccct
     6781 ccagggtctc gttcgagcgt gccgtcacgg gcagggcttc ggtgacaagc gggccgaagc
     6841 gccgaccccg ccaggcgatc gccgccaggg cggtggcgat gagaagaagg gccgtgggcg
     6901 tgacccagcc gggcgtgagg gcggcgaggg tcggccggct cgcggccggc acgtcggcga
     6961 cggtgggcag gtaccagacg agcgtgggcg tctcgcccgc gacgccgatc gcagccgcgg
     7021 cgttgtcggc gcggccgatg ctgccgttct cgaacagttc tgcgctaccg acgatcgtcg
     7081 tgtgcctggg cgaggggagg ctcagcatcg catagccgtc tgccgtgggg aaacagccgc
     7141 cccgaaggcc gtcgccggcc cggtacgagg agccctcgac gagaatccgg cgggcgctgg
     7201 tcgtggcgtc gcccgtgcag tgcgcggtga gcggcgtcgt cggcctgatc gccccgg
//
LOCUS       DASTNL010000175         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1056122, whole genome shotgun sequence.
ACCESSION   DASTNL010000175 DASTNL010000000
VERSION     DASTNL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056122"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..899)
                     /locus_tag="VFQ96_05440"
     CDS             complement(87..899)
                     /locus_tag="VFQ96_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU0257268.1"
                     /translation="MRYDAIVLAGGRSRRLDGVPKEGLLVAGQTLLQHALAAVRGARQ
                     TVVVGPEPAGGLPPSVLQTREDPPFASPAAGVAAGLEALGRPASSKPPDGHRAKRDSV
                     PARPATRVPSPWVLVLACDMPAADAAVAALVAALAGDALADDAPTGEAASDGMIAQDP
                     SGHLQPLAALYRVAALRTAIEAKRAAGSLVGSSMFSLVRPLHLAAIPVPAGSTDDVDT
                     WEDARRLGVRSATPHTMPSTTSLPGASGPAPPEAHEATAPSQRPTQGEESNR"
     gene            1069..>2313
                     /locus_tag="VFQ96_05445"
     CDS             1069..>2313
                     /locus_tag="VFQ96_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0257269.1"
                     /translation="MVLAHRTDLRNVAIVAHVDHGKTTLVDAMLRQTHSFGEHAHVEE
                     RAMDTNELEREKGITILAKNTAIAYNGKHAADGPVTINVIDTPGHADFGGEVERGLSM
                     VDGVVLLVDASEGPLPQTRFVLRKALEARLPVILVVNKTDRADARIAEVVSESQDLLL
                     GLAGDLADDVPDLDLDAILDVPVIYASGRAGAASRNRPANGEMPDNPDLEPLFDAILR
                     HIPAPEYDDEAPLQAHVTNLDASPFLGRIALLRIFNGTLRKGETVAWVRHDGQVHNVR
                     VTELLKTRALDRYPAESADAGDIVAVAGFADIMIGDTLADPDDVRPLPLIHVDEPAIS
                     MTVGTNTSPLAGKVKGHKLTARLVKERLDRELVGNVSLRVLEIGRPDAWEVQGRGELA
                     LAILVEQMRREGYELTVGKPQVV"
BASE COUNT          358 a          815 c          782 g          358 t
ORIGIN      
        1 ctcttcttca cgatcaccgt gctggtggtc gccgtgctgg tgctgcccgt ggccgtcgtg
       61 ctggtggtcg ccgtgctcat ggtcgctcat cgattgctct cttctccttg tgtcggtcgt
      121 tgacttggtg ccgtcgcctc gtgtgcttct ggcggggctg gccctgacgc ccccggcaaa
      181 gacgtggtcg acggcatggt gtgtggggtg gcggaccgca caccgagacg gcgcgcatcc
      241 tcccaggtgt cgacgtcgtc ggtcgagccc gccggcaccg ggatcgcggc gaggtgcagg
      301 ggtcgcacga gagagaacat cgacgagccg acgaggctgc ctgccgcgcg cttcgcctcg
      361 atggccgtgc gcagcgcggc gacgcggtag agcgcagcca gaggctgcag gtggcccgag
      421 gggtcttgtg cgatcatgcc gtcgcttgcc gcctcaccgg tcggggcatc gtccgcgagc
      481 gcatcgcccg caagcgcggc gacgagtgcg gcgaccgcgg cgtcggccgc aggcatgtca
      541 cacgccagca cgaggaccca aggggatggc acacgggtcg ccgggcgcgc cgggacggag
      601 tctcgcttcg ctcgatggcc gtctggaggt ttcgatgacg ccggccggcc caaggcctcc
      661 aagccggccg cgactcccgc ggcgggggag gcgaacggcg ggtcttccct ggtctgcaac
      721 accgagggcg ggaggcctcc ggcaggctct ggcccgacga cgacggtctg tcgggcgccc
      781 cgcacggccg ccagggcgtg ctgcagcagc gtctgcccgg ccacgagcaa gccttctttc
      841 ggcaccccgt cgagacgacg ggatcgcccg ccggccagaa cgatcgcgtc atagcgcacg
      901 gcatcagcct attccggccg acgcgtgccg gggccgtacc cggccaggct gtagaatcta
      961 tcggtgccgc gcggccggcc gggcgcctgc gtgttcgtga tgcccctgcc gcgcatcgca
     1021 cgtggggatg gtgtttcccc ctgctgctca tcgataactg aggaatacat ggttcttgcc
     1081 caccgcaccg atcttcgcaa cgtcgccatc gtcgcccacg tcgaccacgg gaaaaccacc
     1141 ctcgtcgacg cgatgctgcg ccagactcat tcgttcggcg agcacgctca cgtcgaagag
     1201 cgtgcgatgg acaccaatga actcgaacgt gagaagggca tcaccattct cgcgaagaac
     1261 acggcgatcg cctacaacgg gaagcacgcc gccgacgggc cggtgacgat caacgtgatc
     1321 gacacccccg ggcacgcgga cttcggcggc gaggtcgagc gcgggctctc catggtcgac
     1381 ggcgtcgtgc tcctcgtcga cgcgagcgaa gggcccctgc cgcagacgcg gttcgttttg
     1441 cgcaaggcgc tcgaggcgcg tctgcccgtc atcctcgtcg tcaataagac cgaccgggcc
     1501 gacgcccgta tcgccgaagt ggtctccgaa agccaagacc tcttgctcgg ccttgccggc
     1561 gacctcgccg acgacgtgcc cgacctcgac ctcgacgcca tcctcgacgt gcccgtcatc
     1621 tacgcctccg ggcgcgccgg tgccgccagc cgcaaccggc ccgcgaacgg cgagatgccc
     1681 gacaatcccg acctagaacc actcttcgac gccatcctgc gtcatattcc agcaccggaa
     1741 tacgatgacg aggcacccct gcaagcgcac gtcacaaacc tcgacgcttc gccgttcctc
     1801 ggccgcatcg ccttgctgcg catcttcaac ggcacgctgc gcaaggggga gacggtggcc
     1861 tgggtgcgtc acgacggcca ggtgcataac gtgcgcgtca cggagcttct gaagacccgc
     1921 gccctcgacc ggtatccggc cgagtctgcc gacgccggcg acatcgtcgc cgtggcgggc
     1981 ttcgccgaca tcatgatcgg cgacaccctc gccgacccgg acgacgtgcg gccgctgccg
     2041 ctcatccatg tcgacgagcc ggcgatctcg atgacggtcg gcaccaacac gtcacctttg
     2101 gccggcaaag tgaagggtca caagctgacc gcccgcctcg tgaaggaacg cctcgaccgg
     2161 gagctcgtcg gcaacgtgtc gctgcgcgtg ctggagatcg ggcggcccga cgcgtgggag
     2221 gtacaggggc ggggggagct ggcgctcgcg atcctcgtcg agcagatgcg ccgcgagggc
     2281 tacgagctga ccgtcggcaa gccgcaggtc gtc
//
LOCUS       DASTNL010000176         4936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_872678, whole genome shotgun sequence.
ACCESSION   DASTNL010000176 DASTNL010000000
VERSION     DASTNL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872678"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..313
                     /locus_tag="VFQ96_05450"
     CDS             74..313
                     /locus_tag="VFQ96_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEU0257270.1"
                     /translation="MRLGTDALRGAPRTVEGRPRRAGRLFRTLADDAGAATAEYAVAT
                     MAAVGFAGLLVVILRGDEVRGILTDLIRRALTVGD"
     gene            341..649
                     /locus_tag="VFQ96_05455"
     CDS             341..649
                     /locus_tag="VFQ96_05455"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HEU0257271.1"
                     /translation="MTAELAVALPAVLVVLAGCLGCVHVVARQAALVTAAATVARSLA
                     RGDDPAHAGSVLQRLADDASFRHESRGDLVCVDVSASAGLGPSGPRLPLSAHACALAG
                     "
     gene            656..1000
                     /locus_tag="VFQ96_05460"
     CDS             656..1000
                     /locus_tag="VFQ96_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEU0257272.1"
                     /translation="MWCPKGERGAGSVLAVAVLAVVTVFAVALAVASVVAVEGRRAQA
                     AADLAALAAADTASGLVPGIPCDTALRVARANDAALQACALEGQVALVTVEAGARWPV
                     RSTARAGPPGSP"
     gene            1160..4144
                     /gene="topA"
                     /locus_tag="VFQ96_05465"
     CDS             1160..4144
                     /gene="topA"
                     /locus_tag="VFQ96_05465"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU0257273.1"
                     /translation="MPETKKLVIVESPTKVKSIAKYLGDGYEVMASVGHIRDLVEPKN
                     LPVDLKKGDFAKFGVDVEHGFKPYYVVSEQKKKTVADLKRALKGASELVLATDEDREG
                     EAIAWHLLQVLKPKVPVKRMVFHEITREAIERAKEATRELDDALVDAQETRRVLDRLY
                     GYEVSPVLWRKVGPGLSAGRVQSPATRLIVDRERERLAFVAAEYWDLTALFQTGTDGA
                     ADNAAETQRFEARLARLDGTRVAVGRDFDDRGTLTGDARILTGTEADSLVAALSATTA
                     AARVSRVESKPYSRRPAAPFTTSTLQQEAARKLRFSARQTMSVAQSLYENGYITYMRT
                     DSTVLSGQALHAARTQASELYGAESVPAKPRVYTGKSKNAQEAHEAVRPAGETFRTPD
                     ELAQSLRGSEFKLYELIWRRTLASQMADAKGQTASVSIEVTPGAQGTGFEAAEFTASG
                     TVITFRGFLRVYEEGKDEARHAAETADTKLPPLTVGQPLTVTQAEAKSHETTPPPRYT
                     EASLVKALEEMGVGRPSTYAQIIATIIDRGYVTPRGQALVPNWIAFSVVRLLEDYFGD
                     LVEYDFTAGLENDLDKIAAGGENGVDWLHGFYFGDDDHAGLRQVVDNLGEIDARSVNS
                     LAITDGITLRIGKYGPYLEVASEDPLATPRRVNIPRDLAPDELTAEKARELVEAPVVT
                     DRVLGTNPDNGKTVVVKDGRFGPYVTELEPEPAADGPAPAKKTARKEKPRTASLFKTM
                     DPATIDLAGALRLLDLPRVVGKAEDGEEILAQNGRYGAYLKKGTDTRSLAGEDDIFAI
                     DLAGALALFAQPKYRARRAANVLKEFGADPDSGKPITVKDGRFGPYVTDGETNATIPR
                     GEDVDAVDFDRAVQLLADKRAKGPAPKRAARTRSKAGTDRSGAKAKSAAKTGTAKTTT
                     ATTTTAKKPATKKAGSAKTGATKAGAAKAETAPTTAAKSAAKKPAVKKAAASKTGTAT
                     ASASPAGRSKKTPSPSSGPEAQ"
     gene            4141..4767
                     /gene="tmk"
                     /locus_tag="VFQ96_05470"
                     /pseudo
     CDS             4141..4767
                     /gene="tmk"
                     /locus_tag="VFQ96_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
BASE COUNT          824 a         1762 c         1686 g          664 t
ORIGIN      
        1 cggtacggcc accgtgaccg tgccctaccc cgagcacatg catgggacag gtaagagaga
       61 gaggaagacg atcatgaggc tgggaactga cgccctccgt ggcgcgccga gaacagtaga
      121 gggaaggccg cggcgggccg gcaggctttt ccgtacgctc gcagacgacg cgggtgccgc
      181 gacggccgaa tacgccgtcg cgaccatggc cgccgtcggg ttcgcggggc tcctcgtcgt
      241 gatcctgcgc ggcgatgaag tgcggggtat tctcaccgat ctgatccggc gtgcgctcac
      301 ggtcggcgac tgaccgatgc cgtggggcga acggggcacc gtgaccgccg agcttgcggt
      361 ggcgctgccc gccgtgctcg tcgtcttggc gggctgtctc ggctgcgtgc acgtcgtggc
      421 acggcaggcc gcacttgtca ccgccgccgc gacggtcgcc cgttccctcg cccgcggcga
      481 cgaccccgcc cacgctgggt cggtgcttca acggctcgcc gacgacgcat ccttccgtca
      541 cgagagtcgg ggtgacctgg tgtgcgtcga cgtttcggcg agcgccgggc tggggccttc
      601 tggcccccgc ctgccgctga gtgcgcacgc gtgcgccttg gcagggtgag ccgttgtgtg
      661 gtgtccgaag ggagagcggg gtgccgggtc ggtgctcgcg gtcgccgtgc ttgccgtcgt
      721 cacggtcttc gccgtcgcgc tcgccgtcgc gtccgtcgtc gcggtcgagg ggcgtcgggc
      781 tcaggcggcc gccgacctcg cggcgctcgc agccgccgac acggcctccg gtctcgtgcc
      841 gggcatcccg tgcgacaccg cgctacgggt cgcgcgggcg aacgacgccg cgttgcaggc
      901 gtgcgctctc gagggtcagg tcgcgctcgt caccgtcgag gcgggggccc gttggccggt
      961 gcgaagcacc gcacgcgccg ggccgccagg ttcgccgtga gcgaggtatg ggccgtcaag
     1021 catgtgtcgg gatgatatgt gtatggtgtg cctgtcggtt ctgacgggtc gatatgcgtg
     1081 caggagcggg tgccctgaca cgacgatcgc aacccgccca cccgcgtgca agcccgttca
     1141 tgtggcgtaa aggagccctg tgccagagac gaagaaactc gtcatcgtcg agtcgcccac
     1201 caaggtgaaa tccatcgcca agtaccttgg cgacggatac gaggtcatgg cgtccgtcgg
     1261 gcacatccgc gacctggttg agccgaaaaa cctgcccgtg gacctcaaaa aaggagactt
     1321 cgcgaaattc ggcgtcgacg tcgaacacgg cttcaagccg tactacgtcg tcagcgagca
     1381 gaagaagaag acggtcgccg acctgaagcg tgcgctgaag ggcgccagcg aacttgtgct
     1441 cgctaccgat gaagaccgcg agggtgaggc gatcgcctgg catctcttac aggtcttgaa
     1501 gcccaaggtc cccgtgaagc ggatggtctt ccacgagata actcgcgagg ccatcgagcg
     1561 cgccaaagag gccacccgcg agctcgacga cgcgctcgtc gacgcccaag agacgaggcg
     1621 ggtgctcgat cggctgtatg gctacgaggt ttcgccggtg ctttggcgca aggtcgggcc
     1681 gggcctttcc gccggccgcg tgcagtcgcc ggccacccgg ctgatcgtcg accgtgaacg
     1741 ggaacggctc gcgttcgtcg ccgccgaata ttgggatctc acggcgctct ttcagaccgg
     1801 caccgatggc gccgccgaca atgccgccga gacgcagcgc ttcgaggcgc gcctggcaag
     1861 gctcgacggc acacgtgtcg ccgtcggccg cgacttcgac gaccgtggca cgctgaccgg
     1921 tgacgcccgc atcctgaccg gcacggaggc ggattcgctg gtcgcggcgc tcagcgccac
     1981 gacggccgcg gcccgggtca gccgggtgga gtcgaagccc tactcgcgcc ggcctgccgc
     2041 gccgttcacg acctccacgt tgcagcaaga ggcggcccgc aagctgcgtt tctccgcccg
     2101 ccagacgatg agcgtcgccc agtcgctgta cgagaacggc tacatcacct atatgcgcac
     2161 cgactcgacg gtgctctctg gccaagccct tcatgcggcc cgaacgcagg cctccgaact
     2221 gtacggggcc gagagcgtgc cggccaagcc ccgcgtctac accggcaaga gcaagaacgc
     2281 ccaagaggcg cacgaggccg tgcgccccgc cggcgagact ttccgcacac ccgacgagct
     2341 cgcccagagc ctgcgcggca gcgagttcaa gctgtacgag ctcatctggc gccgcaccct
     2401 ggcctcgcag atggccgacg ccaaggggca gaccgcctcg gtgagcatcg aggtgacacc
     2461 cggcgctcag ggtaccgggt tcgaggcggc cgagttcacg gccagcggca cggttatcac
     2521 gttccgcggc tttctgcgcg tctacgaaga gggcaaagac gaagcccgcc acgccgccga
     2581 gactgccgac acgaagctgc cgccgttgac ggtcgggcag cctctcaccg tgacgcaggc
     2641 ggaggcaaaa tcgcacgaga cgaccccgcc gccgcgctac accgaggcga gcctcgtcaa
     2701 ggcgctcgaa gagatgggcg tgggcaggcc ttccacctat gcgcagatca tcgcgacgat
     2761 catcgaccgc ggatacgtca ccccgcgtgg ccaggcgctc gtgccgaact ggatcgcgtt
     2821 cagcgtcgtg cgcctgctcg aggactactt cggcgacctc gtcgaatacg acttcaccgc
     2881 gggcctcgag aacgacctcg acaagatcgc ggccggcggc gagaacggcg tcgactggct
     2941 gcatggcttc tatttcggcg acgacgatca tgccgggctg cgccaggtcg tagacaacct
     3001 cggcgagatc gacgcccgca gcgtcaactc cctggccatc accgacggca taaccctgcg
     3061 catcggcaaa tacggcccct acctcgaggt cgcgtcagaa gacccgttgg cgacgccgcg
     3121 gcgcgtcaac attcctcgcg accttgcccc cgacgagctg acggccgaga aggcccgcga
     3181 gctcgtcgag gcccccgtcg tcaccgaccg ggtgttgggc acgaaccccg acaacggcaa
     3241 gaccgtcgtc gtgaaagacg gccggttcgg cccgtacgtc accgagctcg aacccgagcc
     3301 cgcggccgac ggcccggccc ccgccaagaa gacggcgcgc aaagagaagc cgcgcactgc
     3361 ctccctcttc aagacgatgg acccggcgac gatcgacctc gctggcgccc tgcggctcct
     3421 cgacctgccg cgcgtcgtcg gcaaggcgga agacggcgaa gagatcctcg cgcagaacgg
     3481 ccggtacggc gcctatctga agaagggcac cgacacacgc tccctcgccg gcgaagacga
     3541 catcttcgcc atcgaccttg ccggggcgct cgcgctcttc gcgcaaccca agtaccgggc
     3601 caggcgggcg gcgaacgtgc tcaaagagtt cggggccgat cccgacagcg gcaagccgat
     3661 caccgtgaaa gacggccggt tcggccccta cgtcaccgac ggcgagacga acgcgaccat
     3721 tccgcgcggc gaagacgtcg acgccgtcga cttcgaccgt gccgtgcagc tgttggccga
     3781 taagcgcgcc aaggggccgg cgccgaagcg cgcggcccgc acgaggtcga aggccggcac
     3841 ggatcgatcg ggcgcgaaag cgaagtccgc ggccaagacc ggcacggcca agaccacgac
     3901 cgccacgacg accacagcga agaagccggc caccaagaag gcgggttcgg ccaagacggg
     3961 cgcaacgaag gccggcgcgg ccaaggcaga gaccgccccg acgactgccg cgaagtcggc
     4021 agcgaaaaag ccggccgtca agaaggcggc cgcgtcgaag accggcacgg ccacggcctc
     4081 ggcctcgccg gccggccgct cgaagaagac gccgagcccg tcctccgggc ccgaggcgca
     4141 gtgactctcg gccgggaagg cggcgccttc atcaccttcg aaggggtcga cggctccggc
     4201 aagacgacgc agctcggtct cctcgacgac tggctgcggg gtcgcggcgt gatcacgtgc
     4261 cgcacccggg agccaggcgg caccccggtg ggggaacgga tccgcgagat cgtgctgcat
     4321 catcgcggcg ccatcacccc gcgtgccgag gccctgctct acgcggccga tcgcgcccac
     4381 cacatcgaga ccgtcgtgcg cccggcgttg cagcgcggcg aggtggtcat ccaagaccgc
     4441 tacctcgact cctccgtcgc ctaccagggt tccgggcgga gcctcgaccc tgccgaggtg
     4501 cgaaagctgt cgctgtgggc ggcggacggc ctgctgcccg acctgacgat tcttctcgac
     4561 atcgacgggc gtgccgtcgc cgacaggctc gccggccgca cccggtacga ccgactcgag
     4621 gcagaagacg ccgcgttccg cgaccgggtg cggggggcct accgggcgct cgccgccgca
     4681 gagccggcgc ggttcttcct cgccgacggt gcggcacccg ccgaccgggt cgctcgcgcc
     4741 gtgcagagcc gggtcggcct tctcctcgac ggcgaagatg actgcgctgc acacgcagac
     4801 ccggcaggcc ccgaccccag cggattcgta gacccggcag gctctgccga ccccgccgac
     4861 cccgccgcgc ccgccagccc cgcgaacccc cgcatcgcag gccccagcga cgctcggcgg
     4921 ccgcccaccg ccggcc
//
LOCUS       DASTNL010000177         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1057930, whole genome shotgun sequence.
ACCESSION   DASTNL010000177 DASTNL010000000
VERSION     DASTNL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057930"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFQ96_05475"
     CDS             <1..1194
                     /locus_tag="VFQ96_05475"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU0257274.1"
                     /translation="REYVDLVCSWGPLLLGHAHPAVVEAVQRAASRGLSFGASTPGET
                     ELAEQIVARLSAVTRGGGGSPAGPAVEQVRFVSTGTEATMTAVRLARAVTGRPLVIKF
                     AGHYHGHSDGLLAAAGSGVATLALPASAGVPAEITAQTIVLPYNDRDAVRQAFARYDG
                     RVAGIIVEAAAANMGVVAPLPGFNAFLAEAAHAAGALLIVDEVLTGFRVSPTGWLGLE
                     PVPADLVTFGKVIGGGMPLAALGGRREVMEQLAPTGPVYQAGTLSGNPVAVQAGLATL
                     RAADEAVYSRVDAVAAELSQAVDAALEAQGVPHIVSRAGSLFSVQFRSTLATDYAGVQ
                     EQESFRFGPFFHALLEAGVSLPPSPYEAWFVSAAHDEAALDRVRAALPAAARAAAEAR
                     PAPRA"
     gene            1291..1683
                     /locus_tag="VFQ96_05480"
     CDS             1291..1683
                     /locus_tag="VFQ96_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257275.1"
                     /translation="MADLNVYPDRLQISLTRAERTLARRRRDLVVERGTIASVTITSD
                     PWIWIRGLRDPGVFIPLTLAVGTWKFHGGRDFLLVKGHKRAAVVVDVEGEGVEFGRLI
                     VSTPHAVRLAHVLRLADPHEIDSAGETI"
     gene            complement(1704..2987)
                     /locus_tag="VFQ96_05485"
     CDS             complement(1704..2987)
                     /locus_tag="VFQ96_05485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEU0257276.1"
                     /translation="MSFRAIVIDDSPGAAEQDPPPAPRAPTDTSSPGQAGPGRAPSAG
                     GASRAGRKGVSVGAGRTATLRLLPESFLTGDPAPRVAQTHERPAGRSQDDTGAAASRD
                     PVDGACDKEGTADIDVEYSSLNYKDALALAGRPGIVAHTPLIPGIDLAGVVARSTDRR
                     IRAGDRVVLTGAGAGERMHGGLAERARVPAGSLVCLDDAMTTRHAAAIGTAGFTAMLC
                     VLALERGGLATTSPGKALERGGAEHDDLPILVTGATGGVGSIAVALLARLGHRVTAAT
                     GRVTEHGDYLRRLGADDVIDRSELEKPGRPLQRSRWTGVVDTAGGMILANAIAQTRYG
                     GTVAACGLAHDRDLPASVLPFILRAVTLAGIDSVHAPLALRQEAWRRLARDLDAQLLD
                     DMTTQVSLADVPALAESLLAGRLHGRTLVRVRETG"
     gene            3294..>4036
                     /locus_tag="VFQ96_05490"
     CDS             3294..>4036
                     /locus_tag="VFQ96_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0257277.1"
                     /translation="MRTHQARTHLVGLLGPAFVAAVAYVDPGNVAANLSAGAGYGYLL
                     VWVLVVANVMAAVVQYLSAKLGLVTGRSLPEMLGDRMPHAARLAYWAQGEIVAAATDL
                     AEVVGGAIALQILFAVPLLLGGVIVGVVSLLLLGLQTRHGQRPFELVIMGFLLVITVG
                     FVAGLIVSPLSLAGVVGGLVPRFEGSRSVLLAASMLGATVMPHAIYLHSALERDRHGV
                     VTSPGRKRLLLRAAKVDVTVALAVAGGVN"
BASE COUNT          484 a         1381 c         1503 g          668 t
ORIGIN      
        1 cgggaatacg tcgacctcgt ctgttcctgg ggcccgctgc tcttaggcca tgcgcatccg
       61 gcggtcgtcg aggccgtgca gcgcgctgcg tcgcggggcc tctcgttcgg cgcctccacg
      121 ccgggggaga ccgagctggc cgaacagatc gtcgcgcggc tctcggccgt gacccgtggc
      181 ggagggggat cgccggccgg gccggccgta gaacaggtgc ggttcgtctc gacgggcacg
      241 gaggcgacga tgactgccgt gcgtctggcc cgcgcggtga ccggccgccc gctcgtcatc
      301 aagtttgccg gccactatca cgggcattcg gatggcctgc tcgcggccgc gggcagcggc
      361 gtcgcgaccc tcgcgctgcc cgcctcggca ggggtgcccg ccgagatcac ggcgcagacg
      421 atcgtgctgc cctacaacga ccgcgacgcg gtgcggcagg cgttcgctcg gtacgacggc
      481 cgcgtcgcag ggatcatcgt cgaggccgcc gccgccaaca tgggcgtcgt ggcgccgttg
      541 cccgggttca acgccttttt ggccgaggct gctcatgcgg caggcgcgct cttgatcgtc
      601 gacgaggtgt tgaccggatt ccgggtctcc cccaccggtt ggctcggcct cgagccggtg
      661 ccggccgacc ttgtcacgtt cggcaaggtg atcggcggcg gcatgccact cgcggccttg
      721 ggtggtcgac gggaggttat ggagcagctg gcaccgaccg ggcccgtcta ccaggcgggc
      781 acgctctccg gcaacccggt cgcagtccag gcgggcctcg ccacacttcg cgccgccgat
      841 gaggcggtgt acagcagggt ggatgcggtg gccgccgagc tttcccaggc cgtcgacgcg
      901 gcgctcgagg cacagggcgt gccgcacatc gtctcgcgtg cggggagcct tttcagcgtg
      961 cagttccggt cgacgctcgc gacggattac gcgggggtgc aggagcagga gtcgttccgg
     1021 ttcggcccgt tcttccacgc cctgctcgag gccggcgtta gtctgccgcc gagcccctat
     1081 gaggcctggt tcgtctctgc cgcccacgac gaggcggcgc tggatcgcgt tcgcgccgcg
     1141 ttgccggcgg ccgcgcgggc ggccgcagag gcgcggccgg cgccccgagc ctgaccgtcg
     1201 cccgcgccga gtgccgggct tgtgcgtgcc gtgcgacacg gcggcgatgg gtggagtggc
     1261 tcttcagcgg aggcgggcag actggtaatc atggccgact tgaatgtgta ccccgaccgg
     1321 ttgcagatca gcctgacgcg tgccgagcgc accctcgccc gtcgtcgccg tgatcttgtc
     1381 gtcgagcgcg gcaccatcgc gtcggtcacc ataacctccg acccgtggat ctggattcgc
     1441 ggcctgcgag acccgggagt gttcatcccg ctcactctcg ccgtcggcac gtggaagttt
     1501 cacggcgggc gcgactttct gctcgtcaag ggtcacaagc gtgcggcggt ggtcgtcgat
     1561 gtggagggtg agggggtcga gttcggcagg ctgatcgtca gcacgccgca tgcggtgcgg
     1621 ctcgcacacg tgctgcgcct tgccgacccg cacgagatcg actcggccgg cgagaccatc
     1681 tgacacccgg tgcagccggg ccgttatccg gtctcacgca cgcgaacgag ggtgcggccg
     1741 tgcagtcggc cggcgaggag cgattcggcc agcgcgggca cgtcggcgag tgacacctgc
     1801 gtcgtcatgt cgtcaagaag ctgagcgtcg aggtcgcggg cgaggcgccg ccacgcctct
     1861 tgccgcagcg cgagcggggc atgcaccgag tcgatgcccg ccagggtgac cgctcgcaga
     1921 atgaacggca gcacgctcgc cggcagatcg cggtcgtgag cgaggccgca cgccgcgacc
     1981 gtgccgccgt atcgggtctg cgcgatcgcg ttcgcgagga tcatgccgcc ggccgtgtcg
     2041 accacgcctg tccagcggga gcgctggagc ggccggcccg gcttctctag ctccgagcga
     2101 tcgatgacgt cgtctgcgcc gagcctgcgc agatagtcgc cgtgttccgt gacccgcccc
     2161 gtcgccgcgg tgacccggtg gccgagcctc gccaagagtg cgacggcgat gctgccgacg
     2221 ccgccggtgg cacccgtcac gaggatcggt aggtcgtcgt gttctgcccc gccccgttcg
     2281 agcgccttgc cggggctcgt ggtggccaga ccgccccgtt cgagcgccag cacgcagagc
     2341 atggcggtga atccggcggt gccgatcgcg gcggcgtggc gggtcgtcat cgcgtcgtcg
     2401 aggcatacaa gggagccggc gggcacccgg gcgcgttccg ccagcccgcc gtgcatccgt
     2461 tcgccggcgc ccgccccggt caagacgaca cggtcgccgg cccggatccg ccggtcggtg
     2521 gagcgggcga cgacgccggc taagtcgatc ccgggaatga gcggggtgtg ggcgacgatt
     2581 ccgggtcggc cggcgagggc cagcgcgtct ttgtagttga ggctcgagta ctcgacgtcg
     2641 atgtcggccg tgccctcctt gtcgcaggcg ccgtcgacgg gatcgcggct cgcggcggcg
     2701 ccggtgtcgt cctgagaacg cccggcgggg cgttcgtgag tctgggccac gcgcggcgcc
     2761 gggtcgccgg tcaagaacga ttccggcagc aggcgaagcg tcgccgtgcg gccggcaccg
     2821 acggatacgc ctttccgccc ggcccgtgag gcgccgccgg ctgagggggc gcggcccggc
     2881 cccgcctgcc ccggggaact cgtgtcggtc ggggccctcg gcgccggagg cgggtcctgc
     2941 tccgccgctc cgggagaatc gtcgatgacg atggcgcgaa agctcatgcc tcgagggtac
     3001 ggcggcggcc tcgcgtgcct gtcggacgcc tgggccgcat tccgccgcct gtcggcgggc
     3061 cctgctccgt catgccggcc ccgggcgcct ctgtcgtgcc ggtcctcatg ctgcctccgt
     3121 ctggcggcgc actcgcgccg ggctgcgccg cgacggccga cggatccgcg acgtagggcg
     3181 ggttctgccc gctagaacct tatattcgcc cagcagaagt atgatgttcg tgtcgcctaa
     3241 cattccgggc gtcgggctgg agggcgcaca cggtggcgac cggaacgcaa acggtgcgca
     3301 ctcaccaggc ccgcacacat ctcgtcggcc tgctggggcc ggcgttcgtc gcggctgtcg
     3361 catacgtgga tccgggcaac gttgccgcga acctgtcggc tggcgccggg tacggctacc
     3421 tgttggtgtg ggtgttggtg gtcgcgaatg tcatggcggc cgtcgtgcaa tacctctctg
     3481 ccaagctggg gctggtcacg ggccgcagcc tgcccgagat gctgggcgat cgcatgcctc
     3541 acgcggcgcg gctcgcatac tgggcgcagg gggagatcgt cgccgccgcg accgacctgg
     3601 cggaggtagt gggcggcgcg atcgcgttgc agattctctt cgccgtgccg ttgctcctcg
     3661 gcggggtgat cgtcggcgtg gtgtcgcttc tgctcttggg cttgcagacc cgtcatgggc
     3721 agcgcccgtt cgagcttgtc atcatggggt ttctgctcgt gatcaccgtg ggcttcgtgg
     3781 cgggcctcat cgtctctccg ctcagcctcg ccggggtggt cgggggcttg gttccccggt
     3841 tcgaaggttc ccgtagcgtg ttgctggcgg ccagcatgct cggcgcgacg gtcatgccgc
     3901 acgccatcta cctgcattcc gcgctggagc gcgaccggca cggcgttgtc acgtcgccgg
     3961 gccgcaagag gctgctgctg cgcgccgcga aggtcgacgt gacggtcgca ctggccgtcg
     4021 cgggcggcgt caacat
//
LOCUS       DASTNL010000178         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1430566, whole genome shotgun sequence.
ACCESSION   DASTNL010000178 DASTNL010000000
VERSION     DASTNL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430566"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..864
                     /locus_tag="VFQ96_05495"
     CDS             52..864
                     /locus_tag="VFQ96_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257278.1"
                     /translation="MSDTSASTTRAATAPPLLSLRGISKTFGAVNALSDIDLDVDTGE
                     VVALVGDNGAGKSTLVKILAGAHTRDGGTILWKGKPVTISKPLDSQGLGIAIVYQDLA
                     LCDNLDVVQNLFLGSETGSAFAMDEIDMENRAWGLLRQLSAKIPSIRTPVASLSGGQR
                     QTVAIARSLVGHPQLVILDEPTAALGVAQTAEVLDLVRRLKDNGLGVLLISHNMADVQ
                     KVSDRVAVLRLGRNNGTFATREVSYEDIVTAITGADEVVSSTGAHNVKEEVQ"
     gene            861..2093
                     /locus_tag="VFQ96_05500"
     CDS             861..2093
                     /locus_tag="VFQ96_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0257279.1"
                     /translation="MSAGTLDRQDERLNIGSGFRGELRSAAKSVRQGDIGSLPVIAGL
                     IVVAIVFQTLNPVFLSSQNLVNLALQSTAIGVMAIGVVLVLLLAQIDLSIGSVNGFAA
                     AILAVTFVQWQWPMWLALLVPLAVGAAIGAIYGLLYIRFGVPSFVITLGGLMAFLGFQ
                     LWSLGLNGSINIPFDSFIVRFTQASFLPDVVAYVLVAAIAVGYGASALRSAHRRARQN
                     LSSASATGIYLRGALILVGLGALTWYMNTTRGVPVMFVLFLVLVAATGYALKRTRWGR
                     AIYAIGGNEEAARRAGIHVSRVYMSVFVLSSFLAALGGLLAAGRLASANPSSGGGTTN
                     LEAIAAAVIGGTSLFGGRGTASSAILGILVIESITNGLTLLNMDTSIRYIITGAVLLV
                     AVVIDAISRKSRAAHGRA"
     gene            2220..3029
                     /locus_tag="VFQ96_05505"
     CDS             2220..3029
                     /locus_tag="VFQ96_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0257280.1"
                     /translation="MTKPKYTHPAEGGPRTVRSVVHAVWLLKEISLSPTPSSLGSLAA
                     AIGLSKPATFNLLKTLEVEGLVAKDDAARYRLTWGVYELGSAVVRSADLSRVSRIHLD
                     RLADETGEAVLLAILNERTVLYIDRGQSGDSFAMVANVGRRSPLHTNASGKVLLAGQP
                     DDFVEEILRGPLERHTDFTIVDPVRLRAELSAVRRDGYALCEQEQETGLSSVSVPVTD
                     YTGGVLAALTIAAPSQRIDHESIPAMRDALLRAAQAISTQMGAAADPSTVA"
BASE COUNT          494 a         1116 c         1016 g          529 t
ORIGIN      
        1 gacacggcgc aagccatcaa cacttcgccg gaacccgatt gaggaaatac cgtgagcgat
       61 acgagcgcat ccacaaccag ggccgcgacc gcgccaccgc tgttgtcgct gcgcggcatc
      121 agtaagacgt tcggcgccgt caatgccttg agcgacatcg atctcgacgt cgacaccggc
      181 gaggtcgtcg ccctcgtggg cgacaacggc gccggaaagt cgacactggt gaagattctt
      241 gccggcgcgc acacccgcga cggcgggacg atcctctgga agggcaagcc cgtcacgatc
      301 tcgaagccgc tcgactccca gggtctcggc atcgcgatcg tctatcaaga cctcgcgctc
      361 tgcgacaatc tcgacgtcgt gcagaacctc tttctcggct ccgagaccgg ctccgccttc
      421 gcgatggacg agatcgacat ggagaaccgc gcgtgggggc tcctgcgcca gctctccgcg
      481 aagatcccct cgatccgcac cccggttgcc agcctctccg gcggccagcg acagaccgtc
      541 gcgatcgccc gctcgctggt cggccacccg cagctcgtga tcctcgacga gcccaccgcc
      601 gcactcggcg tggcccagac cgccgaggtg ctcgacctcg tgcggcgcct gaaagacaac
      661 ggcttgggcg tgctgctcat cagccacaac atggccgacg tgcagaaggt gtcggatcgc
      721 gtcgcggtct tacgcctggg tcgcaacaac ggcacgttcg cgacccgcga ggtcagttac
      781 gaagacatcg tcacggcgat caccggagcg gacgaagtcg tttcgagcac cggcgcccac
      841 aacgtcaagg aggaagtcca gtgagtgcgg gtactctcga ccgccaagac gagcggttga
      901 acatcggcag cggattccgc ggtgagttac gctccgccgc gaaaagcgtg cgccaaggcg
      961 acatcggatc cctgcccgtc atcgcgggcc tcatcgtcgt cgccatcgtc tttcagaccc
     1021 tgaacccggt cttcctctcc agccagaatc tcgtcaacct ggcgctgcaa tccacggcga
     1081 tcggcgtcat ggcgatcggc gtcgtgctgg tgctgctcct cgcgcagatc gacctgtcga
     1141 tcgggtcggt caacggtttc gcggccgcga ttctcgccgt gaccttcgtg caatggcagt
     1201 ggccgatgtg gctggcactc ctcgtgcctc tcgccgtggg cgcagcgatc ggcgccatct
     1261 acggtctgct ctacattcgg ttcggggtgc ctagcttcgt cataaccctc ggcggcctga
     1321 tggccttcct cggcttccag ctgtggtcgc tcgggctgaa cggctcgatc aacatcccgt
     1381 tcgactcctt catcgtgcgc ttcacgcaag cgtcgttcct gcccgacgtg gtcgcctacg
     1441 tgcttgtcgc tgcgatcgcg gtcgggtacg gcgcttccgc gttgcgtagc gcccaccgcc
     1501 gcgcccgcca aaacctgtcg tccgcctcgg cgaccggcat ctatctgcgc ggcgccttga
     1561 tcctcgtggg cctcggcgca ctcacctggt acatgaacac gacccgcggc gtgccggtca
     1621 tgttcgtttt gttcctcgtg ctcgtcgcgg cgaccggcta tgcgctgaag cgcacccggt
     1681 ggggccgggc gatctacgcg atcggcggca acgaagaggc ggcgaggcgt gccgggatcc
     1741 acgtctcacg cgtgtacatg tcggtcttcg tgctctcctc gttcctcgcc gccttaggcg
     1801 gcctgctcgc ggctggccgg ctcgcctccg cgaacccgtc gagcggcggg ggaacgacga
     1861 acctcgaggc tatcgccgcg gcggtcatcg gcggcaccag cctcttcggc gggcgcggca
     1921 ccgcctcttc cgcgatcctc gggatcctcg tcatcgaatc gatcacgaac gggctgacgc
     1981 ttctgaacat ggacacgtcg atccgctaca tcatcaccgg cgccgtcttg ctggtcgccg
     2041 tcgtcatcga cgcgatctct cgcaagtcgc gtgccgcgca cggccgcgcc tgagccggaa
     2101 cgatcgggca gccgtgcggg ctgacgcggg cgccagcccg gggcccgtac ggcctcgtac
     2161 ctcgggcggc gtcgcggtga cggggccgaa ggccgtagcg ttgccttgaa ggagcgggag
     2221 tgacgaaacc caaatacacc catccggccg aaggcgggcc gcgcaccgtg cgctccgtcg
     2281 tacacgccgt atggctcttg aaagagatat cgctctcccc caccccgagc tcgctgggca
     2341 gtctcgccgc cgcgataggc ctcagcaagc cggctacgtt caacctcttg aagacgctgg
     2401 aggtggaggg cctcgtcgcc aaagacgatg cagcccggta ccggctgacg tggggcgtct
     2461 acgagctggg cagcgccgtg gtgcgctcag ccgacctcag ccgggtcagt cggatccacc
     2521 tcgaccggct ggctgacgag accggcgagg cggtgctgct ggcgatcctc aacgagcgaa
     2581 cggttctcta catcgaccgg gggcagtcgg gcgactcgtt cgcgatggtc gcaaacgtgg
     2641 gccgccgctc ccccctgcac acgaacgcct cgggcaaggt gctcctggcc ggccagcccg
     2701 acgatttcgt cgaagagatc ctgcgcgggc ctctggagcg gcacaccgac ttcaccatcg
     2761 tcgacccggt gcgcctgcgc gccgagctct cggcggtgcg ccgcgacggc tacgcgctgt
     2821 gcgagcaaga gcaagagacc gggctctcca gcgtctcggt gccggtcacc gactacaccg
     2881 ggggcgtgct cgcggcgttg acgatcgcgg cgcccagcca gcgtatcgac cacgaatcga
     2941 tcccggcgat gcgtgacgcg ttgctccgcg ccgcccaggc catctcgacg cagatgggcg
     3001 cggcggcaga ccccagcacc gtcgcttagg cggagccgaa cggcccgacg agggcgttta
     3061 atgccaccgc gacgccgtcg tcattgttcg tgtcggtgac gaaatcggcg gcccgccgca
     3121 ccggcgcgga ggcgttcgcc ggcgcgacgc cgaca
//
LOCUS       DASTNL010000179         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1245823, whole genome shotgun sequence.
ACCESSION   DASTNL010000179 DASTNL010000000
VERSION     DASTNL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245823"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFQ96_05510"
     CDS             complement(<1..807)
                     /locus_tag="VFQ96_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0257281.1"
                     /translation="MSTRDDYPAGVPCWVDTNQPDPAAAARFYSALFGWEAEDTMPPG
                     SGGHYFMARLTGRVVAAISSQPPESPARAVWNTYVRVESADDTAVRVRAAGGTVISQP
                     FDVFEAGRMAVFHDPQGAVFSVWQPSLHRGAAAVNENGALVFNTLHTDDPDAAAGFYG
                     TVFGWERIGDGPSWWALAGYGDYLEESNPGLRAMLRQSGAPDRFEDVVAALEPRGTGP
                     ARWIVTFAVDDADAAAAHAAKLGGAVITPPQKVPWQRVARLADPAGATFQI"
     gene            complement(881..1330)
                     /locus_tag="VFQ96_05515"
     CDS             complement(881..1330)
                     /locus_tag="VFQ96_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0257282.1"
                     /translation="MARVNLGKAAGELYKTVAALDGQTKDRLKEAGFTEGFCNLLRLR
                     ASQINGCAYCCRLHTLDAAKTGESAERVAVLPAWRETGYFTNEERSALALTEAVTLVS
                     DGQVPDSVYDEAARHLGEAQIAAVEWLAIVMNVWNRIAIPSRYPVAP"
     gene            1493..1939
                     /locus_tag="VFQ96_05520"
     CDS             1493..1939
                     /locus_tag="VFQ96_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEU0257283.1"
                     /translation="MSSSVPRAPRPLHRYWRFVGVVAAGGVVGTACREGFTFAVPEAG
                     SVPWLVFGINVLGALALGFLTAALVRRGADRGVRRVIRLGLGTGLLGGFTTYSTLAVG
                     VASLAQADENWRALAYGLGSVLAGALAAFAGILLAVVVRPGRERAA"
     gene            1936..2310
                     /gene="crcB"
                     /locus_tag="VFQ96_05525"
     CDS             1936..2310
                     /gene="crcB"
                     /locus_tag="VFQ96_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU0257284.1"
                     /translation="MTPVVFVGVCLAGGLGAAARFLLDGWVKARLRVPYPVGTTVINV
                     AGSLVLGAVTGAGLALLPGVAALVGGGFCGGFTTFSTASVETVRLVQDRRWLAGLGNG
                     VGMLVLAVAAALLGLWLGRALA"
     gene            complement(2417..>2811)
                     /locus_tag="VFQ96_05530"
     CDS             complement(2417..>2811)
                     /locus_tag="VFQ96_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257285.1"
                     /translation="PTLPYAAAADAAGQTKARRGTQGGANGTKRAESVEDRAGGAAAG
                     RGPAPERDPKDVLAEAADPATPASRLHELAGAAPYARAALAGNPSTYPALVEWLGRLG
                     DPEVNAALARRAASPADDATTPGGDESA"
BASE COUNT          326 a          977 c         1055 g          453 t
ORIGIN      
        1 gatctgaaag gtggctccgg cagggtcggc aagtctcgcc acgcgctgcc acggcacttt
       61 ctgcggtggc gtgatgacgg caccgccgag cttggcggcg tgtgcggccg cggcatccgc
      121 atcgtcgacg gcgaacgtca cgatccaacg ggccgggccg gtgccccgcg gctcgagcgc
      181 cgccacgacg tcttcgaaac gatcgggcgc acccgattgc cgcaacatcg cgcgcaggcc
      241 ggggttcgac tcttccaggt agtcgccgta cccggccagg gcccaccagg agggaccgtc
      301 gccgattctt tcccagccga acacggtgcc gtagaacccc gcggcggcgt cggggtcatc
      361 ggtgtgcagc gtgttgaaga cgagcgcacc gttctcgttc actgcggccg cgcctcggtg
      421 cagactgggc tgccagaccg agaacacggc gccctgcggg tcgtggaaga cggccatccg
      481 ccccgcctcg aacacgtcga acggctgaga tatgaccgtt ccgccggccg cccgcacccg
      541 gacggcggtg tcgtcggccg actcgacccg aacgtaggtg ttccacacag cccgcgcggg
      601 cgactcgggc ggctgtgagc tgatcgcggc gacgacccgg ccggtgaggc gcgccatgaa
      661 ataatgccct cccgacccgg gaggcatcgt gtcttcggcc tcccagccga agagcgcgga
      721 gtagaaacgg gccgcagccg ccgggtcggg ctggttcgtg tcgacccaac acggcacgcc
      781 ggccggatag tcgtctcgcg tactcatagc cggaccttac gtcgggcgtg gcggcctgtc
      841 gaggggccgg gactgtcgct tttgccgact cgatcgccgg tcagggtgcg accgggtagc
      901 ggctcgggat cgcgatgcgg ttccagacgt tcatgacgat ggcgagccat tcgacggccg
      961 cgatctgcgc ctcgccaagg tgccgggccg cctcgtcgta gacggaatcc ggcacctggc
     1021 cgtcgctcac cagcgtgacg gcctctgtca gagccagcgc ggagcgttcc tcgttcgtga
     1081 agtagccggt ctcacgccag gcggggagca cggcgacgcg ttctgcgctc tctcccgtct
     1141 tcgccgcgtc gagggtgtgc aggcggcagc agtaggcgca cccgttgatc tgcgacgcgc
     1201 gcagcctcag taggttgcag aacccctcgg tgaagccggc ctctttcaga cggtctttgg
     1261 tctgcccgtc gagcgccgcc accgtcttat acagttcgcc ggccgccttg ccaagattca
     1321 cccgcgccat ctctgcaccc ttcgtcgcct cgtctaggcc cctcccgatc cactatgtca
     1381 ccaggccgcc ggcgccggcc tggtgcccgg cgcggagagg agcaggcggc ggtgcggtgg
     1441 gggcggcgcc ggatttccgg cggcacgcgg ccgcgcggca gaatgaacga cgatgtcttc
     1501 ttcagtgccg cgggcgccgc ggccgctcca tcgttattgg cgtttcgtcg gtgtggtggc
     1561 ggccggaggt gtcgtgggca cggcttgccg ggaggggttc acgttcgcgg tgccggaggc
     1621 ggggtcggtg ccgtggctcg tgttcggcat caacgtgctg ggcgccttgg cgctggggtt
     1681 cctgaccgcc gcgctggttc gtcggggtgc ggatcgtggc gtgcgccggg tgatacggct
     1741 gggcctcggc accggcctgc tgggcggatt cacgacgtac agcacgctcg cggtcggggt
     1801 cgcgagcctg gcacaggccg acgagaactg gcgggcgctc gcctacgggc tgggctcggt
     1861 actggccggc gccctggcgg cgtttgccgg aatactcctt gccgtcgtgg tgcggcccgg
     1921 cagggagcgt gccgcgtgac gcccgtcgtc ttcgtcggcg tctgcctggc aggcgggctg
     1981 ggggcggcgg cccggttcct gctggacggc tgggtcaagg cacgcctacg ggtgccatac
     2041 ccggtgggca cgaccgtcat caacgtggcg ggctcgctcg tcttgggcgc ggtgacgggt
     2101 gcgggcctcg cgcttctgcc cggcgtcgcc gcgctcgtcg gcgggggttt ctgcggcggg
     2161 ttcaccacgt tcagcacggc cagcgtggag acggtgcgcc tcgtgcaaga ccgccgctgg
     2221 ctcgcggggc tcggcaacgg cgtgggcatg ctggtcctcg ccgtcgcggc cgccctcctg
     2281 ggcctctggc tcggccgggc cctcgcctga gacggactcg aacgaagggc ggcgcgtctt
     2341 cgaaggccgg aaccgcccgg tgccgaagac cgcgcgcctc gagccgcagc acgacggtcg
     2401 gatcagcggc tgcggctcag gcgctctcgt cgccgcccgg ggtggtggcg tcgtcggcgg
     2461 gcgacgcggc tcggcgggcg agtgcggcgt tcacctcggg gtcgcccagc ctgccgagcc
     2521 attccacgag cgcggggtag gtcgaagggt ttcccgccag ggcggcgcgc gcgtagggcg
     2581 ccgccccggc gagctcgtgc agccgcgacg cgggtgtcgc cgggtcggca gcctccgcga
     2641 ggacgtcctt cgggtcgcgt tcgggtgccg ggccccggcc cgctgcggcg cccccggccc
     2701 tgtcttcgac agactcggcc cgcttcgtgc cgttcgcccc gccctgcgtg ccgcgtcgcg
     2761 ccttggtctg gcccgcggcg tctgcggccg ccgcgtaggg cagcgtcggt g
//
LOCUS       DASTNL010000180         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1060056, whole genome shotgun sequence.
ACCESSION   DASTNL010000180 DASTNL010000000
VERSION     DASTNL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060056"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFQ96_05535"
     CDS             complement(<1..234)
                     /locus_tag="VFQ96_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEU0257286.1"
                     /translation="MSAPWTAPPLSPDGGTARSWLIGELSKPAYQAARPTWFDVLSAR
                     LKDWVTGLFTGAQAPLTPLLAALGIAIAAAIVIG"
     gene            complement(231..1415)
                     /locus_tag="VFQ96_05540"
     CDS             complement(231..1415)
                     /locus_tag="VFQ96_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257287.1"
                     /translation="MSDDTGWAAPGGRHPLPLPGYGPPPGAAAGPPSGPGWSPPPSPG
                     LMPLHPLGFGTLLGIPFRELRRNPRPVVGTSLIVQAVVMIASLFVTGAVGLTAYDRVA
                     MAAPAERATVLSGAVAGFVLSLLVPVVLGLLGSAFLQGVIVADVARGTLGERLSLRQL
                     WHAARRRLAPLVGWVLLLAAGITVLVAAVAAIVWLSLTAGGPAMIIGIVAAVFVGIAA
                     LIVGVWLYVKTSLVPCLIVLERRGVRSSLGRSWLLTGGLFWRMFGVQALVAVIVNAIS
                     QVATTPLGLAVGIVGGIVAPTGSAAGGGGAESGTGRAALIALYAATLLVTLLVSALTT
                     VVQSAAVAVLYLDARMRKEGLALHLTRYVEERAAEREDPPDPFETPAAGAAPKGSSGE
                     VS"
     gene            1617..2294
                     /gene="mtrA"
                     /locus_tag="VFQ96_05545"
     CDS             1617..2294
                     /gene="mtrA"
                     /locus_tag="VFQ96_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HEU0257288.1"
                     /translation="MNGRILVVDDDAALSEMIGIVLSTEGFAVSFCVDGSRALAAFRQ
                     DEPDLVLLDLMLPGIDGIQVCRLLRAESGVPVIMLTARADTADVVKGLESGADDYVVK
                     PFNPKELVARVRARLRPSVNESALTTLRVADVEIDVAAHEVRRGERRVNLTPLEFDLL
                     VALASRPDHVFTRETLLEKVWGYRYKADTRLVNVHVQRLRAKVEDDPENPRVVITVRG
                     VGYRAAG"
     gene            2299..>3783
                     /gene="mtrB"
                     /locus_tag="VFQ96_05550"
     CDS             2299..>3783
                     /gene="mtrB"
                     /locus_tag="VFQ96_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEU0257289.1"
                     /translation="MVALPTLVPPRRNRLLGALRHSLRLRTVAITVLLTGAAIVAAGL
                     AVSYSVAGDLYSSRLSQALHDSARATASAQATVDAADISESGAMRHLLDTALGAIGTA
                     TSSRLVASYRMPGQQATSLAPQDFRSPGLAPADISPELRAAVQNGSGKQFYQSVRLTG
                     TGGQEDPGIVVGSQLRLPPPAGRYELYIGYDLSAAAATLAFVQRTLLIAGIALIVLMA
                     AITWVIVRFVVQPIKLAAETSKRLAAGELGVRIPERGADVFAALAASFNGMADSLQHQ
                     ITRLAALSRLQQRFVSDVSHELRTPLTTIRLAEGVIYDQRESLDPAAQRSAELLHAQV
                     ERFGRLLVDLLEISRYDAGSAVMELEQVDLAQLARTVVGEMRPLAARHDTRLLVSGEP
                     GATASLDARRIRRIIRNLIGNAIEHGEARPIRVRVAYDESSVALSVRDQGVGIAADQV
                     GHVFDRFWRADRSRQRTIGGTGLGLAISMEDAIAHHGTLEVWSRP"
BASE COUNT          579 a         1368 c         1337 g          499 t
ORIGIN      
        1 gccgatcacg atcgccgccg cgatcgcgat cccgagcgcc gcaaggagcg gcgtcagcgg
       61 ggcctgcgct cctgtgaaga ggcctgtcac ccagtctttg agccttgcgg agagcacgtc
      121 gaaccaggtc ggccgggccg cctgataggc aggtttggag agctctccga tcagccagga
      181 gcgggccgtc ccgccgtcgg gcgagagcgg cggcgcggtc cacggcgcgc tcacgagacc
      241 tcgcccgaag accctttcgg ggcggcgccg gccgccgggg tctcgaacgg gtcgggcggg
      301 tcctcccgct cggcggcgcg ctcctcgacg taccgggtga ggtgcaacgc caggccctct
      361 ttgcgcatgc gtgcgtcgag gtagagcacc gcgaccgcgg ccgactgcac caccgtcgtc
      421 agcgccgaca cgaggagtgt cacgaggagg gtcgccgcat agagtgcgat gagagcggcc
      481 cgtcccgtgc ccgactcggc cccgccgccc cccgccgcgg acccggtggg tgcgacgatg
      541 ccgccgacga tgccgacggc gaggccgagc ggcgtcgtcg cgacctgcga gatcgcgttc
      601 acgatcaccg cgacgagggc ctgcacgccg aacatccgcc agaagagccc gcctgtcaga
      661 agccaggagc gcccgagcga ggagcgcacg ccgcggcgct ctaaaacgat gaggcagggc
      721 acgagcgacg tcttgacgta gagccacacc ccgacgatga gggcggcgat gccgacgaac
      781 acggccgcga cgatgccgat gatcatggcg ggcccgcccg cggtgagcga cagccagacg
      841 atcgccgcga cggccgcgac gagcaccgtg atgccggccg cgagaagcag cacccagccg
      901 acgagcggcg ccagccgccg cctggccgcg tgccacagct ggcgcagcga caggcgttct
      961 ccgagagtgc cgcgggcgac gtcggccacg atgacaccct gcaagaacgc ggagcccaga
     1021 aggccaagca ccaccgggac gagaagggag agcacgaagc cggccacggc accgctcaac
     1081 acggtggcgc gttccgcagg tgccgccatc gccacccggt cgtatgcggt gagccccacc
     1141 gcgcccgtca cgaagagcga ggcgatcatc acaacggcct gcacgatcaa ggaggtgccg
     1201 acgaccggcc gagggttgcg tcgcagctcg cgaaacggaa tccccaacag cgtgccgaag
     1261 ccgagcggat gcagcggcat caggccgggg gagggcggcg ggctccagcc cggccccgaa
     1321 ggcggcccgg ccgcggcgcc gggaggcggt ccgtagcccg gaagggggag gggatgccgg
     1381 ccgccggggg cggcccaacc ggtgtcgtcg ctcacgcgct gaccccctcg agtgaagccc
     1441 tggccggcag gcgcggtctc ttaccatgct gccacacgtg ggcccctgct cggctaatgt
     1501 ggaggggcag acaacggtgg cgtggaacgc ccgcgtgcgt cgagacggcc ggcggcacgt
     1561 ccccagccga ccgcggcagg ctccccgacg acgcggcgcc gggtgaggaa gggctcatga
     1621 acggtcgcat cctggtggtc gacgacgacg ccgcactctc cgagatgatc ggcatcgtct
     1681 tgagcaccga aggtttcgcc gtctcgttct gcgtcgacgg ttcccgggcc ctcgccgcct
     1741 tccggcagga cgagcccgat ctcgtgctgc tcgacctgat gttgcccggc atcgacggaa
     1801 tccaggtgtg ccggctgctc cgggccgagt cgggggtgcc ggttatcatg ctgacggccc
     1861 gcgccgacac ggcggacgtc gtgaaggggc tcgagtcggg cgccgacgac tacgtcgtga
     1921 agccgttcaa cccgaaggag ctcgtcgcgc gggtgcgtgc ccgtctgcgg ccgagcgtca
     1981 acgagtcggc tctcacgacg ctgcgggtcg ccgacgtcga gatcgacgtg gccgcgcacg
     2041 aggtgcggcg tggagagcgg cgcgtcaacc tcacgccgct ggagttcgac ctgctcgtcg
     2101 cactcgcctc ccggccggac cacgtcttca cccgcgagac ccttctcgag aaggtgtggg
     2161 gttaccgcta caaggcagac acgcggcttg tgaacgtgca cgtgcaacgc ctccgggcga
     2221 aggtggaaga cgacccggag aacccccgcg tcgtcatcac ggtacgcggc gtcgggtacc
     2281 gcgccgcggg gtgagatcgt ggtcgccctc cccacgctcg tgccccccag acgcaacagg
     2341 ctccttggcg ccctgcggca ttccttgcgg ctgcgcacgg tggcgatcac cgtgctcttg
     2401 acgggcgccg cgatcgtcgc cgccggcctc gccgtctcgt acagcgtcgc cggagacctc
     2461 tattcgtcgc gcctcagcca ggcgttgcac gactccgcgc gggccaccgc gtcggcgcag
     2521 gcgaccgtcg acgcggccga catctcggag agcggggcga tgcggcacct gctcgacacg
     2581 gcactcggcg cgatcggcac cgccacgtcg agccgtctcg tcgcctcgta ccgcatgccc
     2641 ggtcagcagg ccacgtcgct ggccccgcaa gacttccgca gccccggcct cgcccccgcc
     2701 gacatctcgc cggaactgcg ggcggccgtg cagaacggct cgggcaagca gttctaccag
     2761 tcggtgcgcc tgacgggcac cggcgggcag gaggatccgg gcatcgtcgt cgggtcgcag
     2821 ctgcggctgc ccccgccggc cggccgctac gagctttaca tcggctacga cctcagcgcc
     2881 gcggccgcca ccctcgcgtt cgtgcaacgc accctgttga tcgccggtat cgcactcatc
     2941 gtgctcatgg ccgcgatcac ctgggtcatc gtgcgtttcg tcgtgcagcc catcaagctg
     3001 gcggccgaga cgagcaaacg gctggccgcg ggcgagctcg gcgtgcgcat cccggagcgc
     3061 ggcgccgacg tcttcgcggc gttggccgcc tcgttcaacg gcatggccga cagcctgcag
     3121 catcagatca cgcggctggc cgcactctcc cggctgcagc agcgtttcgt ctccgacgtc
     3181 tcccacgagc tgcgcacacc gctcacgacc atccggcttg cggagggcgt gatctacgac
     3241 cagcgggagt cgctcgaccc ggcggcgcag cgcagcgccg aactgcttca cgcccaagtg
     3301 gagcgcttcg gccgccttct cgtcgacctt ctcgagatca gccgctacga cgcgggttcc
     3361 gccgtgatgg agctggagca ggtcgacctg gcccagctgg cgcgaacggt cgtcggcgag
     3421 atgcggccgt tggcggcccg gcacgacacg aggcttctcg tctcgggaga acccggcgcg
     3481 acggcgagcc tcgacgcccg ccgcatccgc cgcataatcc gcaaccttat cggcaacgcc
     3541 atcgagcacg gcgaggcccg ccccattcgg gtgcgggtcg cctacgacga gtcgagcgtg
     3601 gcgctgtcgg tgcgcgacca gggggtcggc atcgccgccg atcaggtcgg tcacgtgttc
     3661 gaccggttct ggcgggcgga ccgctcgcgc caacgcacga tcggggggac cggcctcggc
     3721 ctcgccatct cgatggaaga cgcgatcgcc caccatggca cccttgaggt gtggtcgagg
     3781 ccg
//
LOCUS       DASTNL010000181         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1060293, whole genome shotgun sequence.
ACCESSION   DASTNL010000181 DASTNL010000000
VERSION     DASTNL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060293"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFQ96_05555"
     rRNA            <1..428
                     /locus_tag="VFQ96_05555"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            508..624
                     /gene="rrf"
                     /locus_tag="VFQ96_05560"
     rRNA            508..624
                     /gene="rrf"
                     /locus_tag="VFQ96_05560"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            870..1388
                     /locus_tag="VFQ96_05565"
     CDS             870..1388
                     /locus_tag="VFQ96_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257290.1"
                     /translation="MGAARAEAVTSIGVILRADVAARAEEAVRIARIAAIGRAVVTLP
                     TEVIVLAAPVGQNVAIALTEVVARARVAARAGAVRWRGAPLVLNAARLAAVDGQASKG
                     RKHGPGEVAAPLGEPGAVEARPGVRVVSGIPACDARGLRATVGRARDARRGRRCPTTC
                     RPVISTVPCGCS"
     gene            1346..2119
                     /locus_tag="VFQ96_05570"
     CDS             1346..2119
                     /locus_tag="VFQ96_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257291.1"
                     /translation="MSARDLDRSVRVQLKGLSKENAEAVARHLVMVGRLIESDPAEAH
                     RHALAAAERAGRIAVVRETVGITAYEVGDFARALRELRTYRRISGRDDEVALMVDSER
                     GVGRPDRALEIGRAVAAGALSPAVRVALAIAMSGARLDLGQTREARGELEIPQLDETK
                     VFDYSPQLFDAYAEVLEELGDGAAAGWRRRAVTARSALYTASQAAADETVAVVDEVTD
                     EASDSGALDRAVAAGGDRAAVGNDAEAAGRGPGDGQDPA"
     gene            2116..>2785
                     /locus_tag="VFQ96_05575"
     CDS             2116..>2785
                     /locus_tag="VFQ96_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0257292.1"
                     /translation="MMGRRGVGKTPVGDVDVLLTDLDGVVYRGLEAVRYAVEGLERAA
                     GSMRVGYVTNNASRTDAAVARQLSSFGLTVAAGDVVTSPQAAVRLLRDLVPTGAVVLA
                     VGGDGVVDELTKAGYRPTRSALDRPAAVVQGFATDIGWKELAEASYALQGPEGDEIPW
                     IATNTDWTLPTSRGIAPGNGTLVSAVHSAVGRLPVFAGKPEAAIFDIARERFAPQRVL
                     FLGDR"
BASE COUNT          416 a          895 c         1041 g          433 t
ORIGIN      
        1 tatcgacggc atggtttggc acctcgatgt cggctcgtcg catcctgggg ctggagttgg
       61 tcccaagggt tgggctgttc gcccattaaa gcggtacgcg agctgggttt agaacgtcgt
      121 gagacagttc ggtccctatc ctctgtgcgc gttggagatt tgagaggatc cgaccctagt
      181 acgagaggac cgggttagac gaacctctgg tatgccagtt gttccgccag gagcatggct
      241 ggttagctac gttcgggatg gataaccgct gaaagcatct aagcgggaag ccggcctcga
      301 gatgagatct ccatgccttt atggtgagag gtccccagcc agacgactgg gttgataggc
      361 gggatgtgga agcgaggact aaagactcgt ggagcttacc cgtactaata gaccgatgac
      421 ttgataacac attgcttgcg tccactttgt ggttcccgat gtacggccgg gaacacgcga
      481 tattcgcgtt cgatacatca acagtgtttc ggtggtcata gcgagaggga aacgcccggt
      541 aacattccga acccggaagc taaggctctc agcgccgatg gtactgcagg ggggaccctg
      601 tgggagagta ggacaccgcc ggactaattg tgacgtggcc acccttcggg gtggccacgt
      661 gtcgttaagg agaatcatgc cggtgcacga cgggtcgggc ggcgacgcct ccggtgaggg
      721 tgttgctccg agaccgtcgg gcaattcgga tgcgcggaat ggaaaaggcg gcgccgagcg
      781 tcgccgcgag tcatctgtcg gcgggccccg cgagcgtggg cgcgcttcgt cgggtggtcg
      841 gcgctcatcg gccgacgacg gcgtgaacca tgggggccgc acgagccgag gcggtcacgt
      901 cgatcggggt tatcctgcgg gcggacgtgg ccgcccgggc cgaggaggcc gtacgaatcg
      961 cccggatcgc ggcgatcggg cgcgccgtgg tgacgctgcc gaccgaggtg atcgttctgg
     1021 ccgcgccggt cggccagaac gtggcgatcg cgttgaccga agtggtcgcg cgggccaggg
     1081 tggccgcccg ggccggggcg gtcaggtggc ggggcgcgcc cctggtgctg aacgcggcga
     1141 gactcgcggc cgtcgacggg caggcgagca agggtcggaa gcacggtccc ggggaggtcg
     1201 cggcgcccct gggggagccg ggcgccgtag aggcgaggcc cggcgtccgg gtggtatcgg
     1261 gcattccggc ctgcgacgcc cggggcctgc gagcgacggt cgggcgcgcc cgcgacgcga
     1321 ggcggggccg gcgctgcccg acgacgtgtc ggcccgtgat ctcgaccgtt ccgtgcgggt
     1381 gcagctgaag gggctcagca aggagaatgc cgaggccgtg gcccggcacc tcgtgatggt
     1441 cggccgcctc atcgagagcg acccggccga ggcgcatcgg catgccctcg ccgcggcgga
     1501 acgtgccggc cgcatcgcgg tggtgcgcga gacggtcggc atcaccgcct acgaggtcgg
     1561 cgacttcgct cgcgccttgc gggagttgcg cacctaccgg cgcatcagcg gccgggacga
     1621 cgaggtggcg ctcatggtgg actccgagcg cggcgtgggc cggccggatc gtgcactcga
     1681 gatcggtcgc gccgtcgctg ccggggcgtt gtcgcccgcc gtgcgggtcg ctctggccat
     1741 cgccatgtcg ggggcgcgcc tcgacctcgg gcagacgcgc gaagcccgcg gcgagctcga
     1801 gataccgcaa ctcgacgaga ccaaagtctt cgactattcg ccccagctgt tcgacgcgta
     1861 cgccgaggtc ttggaggagc tcggcgacgg ggcggccgcg ggctggcggc gccgcgccgt
     1921 cacggcacgc tccgccttgt acacggcgtc ccaggcggcg gccgacgaga cggtggccgt
     1981 cgtcgacgag gtgacagacg aagcatccga cagcggcgcc ctcgatcgcg ccgtcgcggc
     2041 cggcggcgac agggcggcgg tcggtaatga cgcggaggcg gccggccgcg gcccgggcga
     2101 cgggcaggat ccggcatgat gggccggcga ggtgtcggca agaccccggt cggagacgtc
     2161 gatgtgctct tgaccgacct cgacggcgtc gtctaccgcg gcctcgaagc tgtccggtac
     2221 gcagtcgaag gtctcgaacg tgccgccggc agcatgcggg tcgggtacgt gacgaacaac
     2281 gcctcccgca ccgacgctgc cgtcgcccgc caactgtcgt ccttcgggct cacggtcgcg
     2341 gcgggcgacg tggtcacctc gccccaggcc gccgtgcgcc tgttgcgcga tctcgtgccg
     2401 accggcgcgg tcgtcctcgc ggtcggcggc gacggcgtcg tcgacgagtt gacgaaggcc
     2461 gggtaccgcc cgacccgttc ggcgctcgac cggcccgccg ccgtcgtgca gggcttcgcc
     2521 accgacatcg ggtggaaaga gctcgccgag gcctcctacg ccctgcaagg ccccgaaggc
     2581 gacgagattc cgtggatcgc taccaacacg gattggacgc tgcccacctc gcgcggcatc
     2641 gcgcccggca acggcacact cgtctccgcc gtgcacagcg ccgtcggccg cctgccggtg
     2701 ttcgccggca agcccgaagc cgccatcttc gacatcgccc gcgaacggtt cgcgccgcag
     2761 cgtgtccttt ttctcggtga tcggc
//
LOCUS       DASTNL010000182         6126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_691680, whole genome shotgun sequence.
ACCESSION   DASTNL010000182 DASTNL010000000
VERSION     DASTNL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691680"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFQ96_05580"
     CDS             <1..378
                     /locus_tag="VFQ96_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257293.1"
                     /translation="PAATAVFTPPPAVEALRVVFETVFLVAAAPFRAEDDPADFALTA
                     VVAESTAVAAPFRCASFSAEAPDVACAASGAARRCERAGALAFRVATFAAADAAVPVE
                     AASGSDTAVAWETESAFIARVAM"
     gene            complement(617..1543)
                     /locus_tag="VFQ96_05585"
     CDS             complement(617..1543)
                     /locus_tag="VFQ96_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEU0257294.1"
                     /translation="MAHNELYETVIGASVVPDGLHLVAGLTGFADAGAAVAQFTSQVL
                     QNLESEVVAQFDADELLDYRARRPVAYFDEDHLADFQPLSLTLRLVRDEVGQPFLLLS
                     GFEPDFKWQRFSQAVLAIVERYRVKSLTWLNSIPMPVPHTRPISVTVSGNRHDLIERM
                     SVWRPRTQVPSTVLHLVEYRMQQAGYPVVGFVILVPHYLSDTEYPAAAISAMESVGVS
                     TGLMLPTTGLRDADRDFVANIDEQVRTNGELTRLVKALEERHDKYMQDNPLQPSLTDE
                     DGELPTADEIAAELEDFLAFRRHGDDDGGRTQ"
     gene            1781..3154
                     /gene="lpdA"
                     /locus_tag="VFQ96_05590"
     CDS             1781..3154
                     /gene="lpdA"
                     /locus_tag="VFQ96_05590"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0257295.1"
                     /translation="MSEQNFDVVVLGGGSGGYAAALRASELGFSVALIEKDKLGGTCL
                     HRGCIPTKALLHAAEVADAARGSDRFGVKTSFEGIDIAAVTSYREGVVGNKFKGLQGL
                     IKARGITTVAGLGTLTAANTVSVGADQYIGKNIVLATGSYSKSLPGLDITGRVITSEQ
                     ALELDYIPSKVAILGGGVIGVEFASVWKSFGAEVTIIEALPHLVPNEDDSVSRQLERA
                     FRRRGIDFRLGIRFDSVTQSDQGVHVTLADGNTVDADLLLVAVGRGPVTANLGYEEAG
                     IAMERGYVLTDERLHTNVPGVYAVGDIVPGLQLAHRGFQQGIFAAEEIAGLNPIVVDD
                     ANIPKVTYCDPEVASIGLSEAKAKDAYGADKVVSYDYNLAGNGRSEILGTTGSVKVVR
                     VAGGPVVGVHMIGSRIGELIGEAQLVVNWEAYPEDVAPFIHAHPTQGESLGEAFLHLT
                     GKALHTL"
     gene            3255..4688
                     /locus_tag="VFQ96_05595"
     CDS             3255..4688
                     /locus_tag="VFQ96_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU0257296.1"
                     /translation="MSETVSLPALGESVTEGTVTRWLKNVGDRVEVDEPLLEVSTDKV
                     DTEVPSPISGVVEEILVAEDETVDVGTVLVRIGDGSGQAAAQASAEPAAAPERQAPAP
                     QPAAAEPAPVAAAPLAPAAPTAAPVQPTYTPPAPVAAPQPTAPQPVVQAPLPPRAAAP
                     APAAQASTPTPAAAQAPATPTTGAPAPRGAAHAGQYVTPIVRKLASEQGVDLDTVAGS
                     GVGGRIRKEDVLQAATRAEVVDSGIRGTREAMSALRRVTAERAVASLRESAQATCVKE
                     VDVTDLMRTIAASAAQFERANTVPLGFLPFVVRATAEALRQHPMVNASVTGDTIVYPE
                     DENICLTVDTDKGILTPVLRGAANLSIAQVAKEIVEVTGRVAANTLRPDELVGGTFTI
                     TDSGARGALFDTPVVFLPQVAALGVGEVTKRPAVVSVAGTDAIAIRSSVFFSLSYDQR
                     IIDSAQAARFLTTLAGSLGSAAVDGVL"
     gene            complement(4727..>6126)
                     /locus_tag="VFQ96_05600"
     CDS             complement(4727..>6126)
                     /locus_tag="VFQ96_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257297.1"
                     /translation="HVVALRDFTWLADGYGALVLEWVAGPSVTEWLDRRHRVTPGEAT
                     TVVVSVLRAEGALRAAGWASDGGPSPSRFRLDGDGRPVLVGLGGLVRRGAGGTDDESR
                     AVFDDDVEDGLDRGVRALLHSVALRLPPGMRDRWRGAVLHAWHPERLPGQAPPHGLLV
                     AGPGGSDGVDAPDADDPGSTSDAGSALEALEDAVFAYAPPEPLTTQVEEPEAAQGGAA
                     PTADSDTGTGFDLGPDTPAQPSAYGRPLAFETRGSSLIDQARNVIEPLIRGLRRRRRP
                     LIVAGVAGAVALAAALAALPLGSAPAPSTGAPGDAAASTGRGATDRDSGAAGAAGTTS
                     PSPSEATGVGGTAEDTATPSGRDAGAAADPASAAVALLLARERCFSSGSAACLARVDE
                     PGSPQARADAAVLAGTAEHKPVTYAGREAALVQRNGGAALVSVDPAAGTKNAPPLSVL
                     VMETESGWALREVFG"
BASE COUNT          980 a         2147 c         2089 g          910 t
ORIGIN      
        1 ccggcggcca cagccgtctt caccccgccg ccggcagtgg aggctttgcg cgtcgtcttc
       61 gaaaccgtgt tcctcgtcgc ggcagcgccc ttccgggcag aagacgaccc ggcagacttc
      121 gccttgacgg cggtcgtcgc ggaatcgacg gccgtggccg caccctttcg ttgtgcgtca
      181 ttctcggccg aagcccctga cgtcgcctgt gcagcttcgg gtgccgcgcg ccggtgcgag
      241 cgcgccggcg ccttggcctt ccgcgtcgcg accttcgccg cggcggatgc tgccgttccg
      301 gtcgaagccg cctccggctc cgacacggca gtcgcctggg agacagagtc ggccttcatc
      361 gctcgggttg ccatgtaaac acctttcatg ccccgcgcag ggtgtccggc tctcgtccgt
      421 ggcggctggg catgggattg ttttcggaca ctatgaggac ccatgtcaag tcccccgcga
      481 cgcgtcgtcg aaacgactgc gcgatgaagg cctgaacggg tccgaacctc aattattgca
      541 tgaatcacgc gccgggcagg ccgcacggcc aaacctcaca gggggcgcgc tgaacaccct
      601 ctgccgcgcg gcctcactac tgcgtacgac cgccgtcgtc gtcgccgtgc cggcgaaagg
      661 cgaggaagtc ttcgagttct gccgcgatct cgtcggcagt cggcagctca ccgtcttcat
      721 cggtcagcga cggctgcagc ggattgtctt gcatgtactt gtcgtgccgc tcttccagcg
      781 ccttgacaag acgcgtgagc tctccgttcg tgcgaacctg ctcatcgatg ttggccacga
      841 aatcccggtc ggcgtcacgc aagcccgtcg tgggcagcat gagccccgtc gagacgccga
      901 cgctctccat ggccgatatc gcggccgccg ggtactccgt gtctgagagg taatgcggca
      961 ccaggatgac gaagccgacc accgggtaac cggcctgctg catccggtac tcgacgagat
     1021 gcagcaccgt cgagggcacc tgggtgcgtg gccgccacac cgacattcgc tcgatgaggt
     1081 catgtctgtt gcccgagacg gtgacgctga tcggccgggt atggggcacc ggcatgggga
     1141 tcgagttcag ccaggtaaga ctcttgacgc ggtaacgctc cacgatcgcc agcaccgcct
     1201 ggctgaaccg ctgccacttg aagtcgggct cgaaaccgct cagcaacaga aagggctggc
     1261 cgacctcgtc acgcacgagg cgcaacgtca ggctgagagg ttgaaaatcg gcgaggtggt
     1321 cttcgtcgaa gtaggcgacc gggcgcctcg cgcggtagtc gagaagctcg tcggcgtcga
     1381 actgtgccac gacctccgac tcgaggtttt gcagcacctg agaggtgaat tgcgcgaccg
     1441 cggcccccgc gtcggcgaaa cccgtcagcc cagccacgag atgcagcccg tcggggacga
     1501 cgctcgcccc gatcacggtc tcatacaact cgttgtgcgc catgcgtcca gtttaatcgg
     1561 cagcctccgg caccgcctcg ggcgggcgga cccacgcaga cgccgtcagc gaacatgacg
     1621 gccggcgccg gaggcggcgc ccgggatccc cgccggcgcc gggtagtaag gttttacccg
     1681 gcgggtccgg cccgtcgccg gccgggatcc ggctgagcac agcctcggga tcaggccatg
     1741 gatcgaagca gggaaagctt gcgtacagag gagtctgcgg gtgtcggaac agaactttga
     1801 cgtcgtcgtt ctcggtggtg gcagcggggg ctacgccgcc gccctccggg ccagcgaact
     1861 gggcttcagc gtcgcgctga tcgagaagga caagctcggc ggcacctgcc tgcaccgagg
     1921 ctgcatcccg acgaaggccc tccttcatgc ggccgaagtg gccgacgcag cccggggctc
     1981 cgatcggttc ggcgtgaaga catcgttcga ggggatcgac atcgccgcgg tgacgtcgta
     2041 ccgagaaggc gtcgtcggca acaagttcaa gggcctgcag gggctcatca aggctcgcgg
     2101 catcacgacg gtcgcgggcc tcggcacgct caccgccgcc aacacggtga gcgtcggagc
     2161 cgaccagtac atcggcaaaa acatcgtgct cgccaccggc tcctattcga agtcgctgcc
     2221 ggggctcgac atcaccggcc gcgtcatcac gagcgagcaa gcactggagc tggactacat
     2281 cccctctaag gtggccatcc tgggcggcgg cgtcatcggc gtcgagttcg cgagcgtctg
     2341 gaagtcgttc ggtgccgagg tgacgatcat cgaggccctg ccgcatctcg tgcccaacga
     2401 agacgactcg gtcagccggc aactggagcg tgcgttccgg cgccgcggca tcgattttcg
     2461 actcggcatc cgtttcgact cggtgacgca gagcgaccag ggtgtgcacg tgacgctcgc
     2521 cgacggtaac accgtcgacg ccgaccttct tctggtcgcg gtcgggcgcg gcccggtgac
     2581 ggcgaacctc ggctacgaag aggccggcat cgcgatggaa cgcggctatg tcttgactga
     2641 cgaacgcctg cacacgaacg tgccgggcgt ctacgccgtc ggcgacatcg tgccggggct
     2701 gcaactcgca caccgcgggt tccagcaggg tattttcgcg gcggaagaga tcgcgggcct
     2761 gaacccgatc gtcgtcgacg acgccaatat tcccaaggtg acgtactgcg accccgaggt
     2821 ggcctccatc ggtctctcgg aggccaaggc caaagatgcc tacggcgcgg acaaggtagt
     2881 cagctacgac tacaacctcg ccggcaacgg ccgcagcgag atcctgggga cgaccggttc
     2941 ggtgaaggtc gtgcgcgtgg cgggcgggcc cgtggtcggc gttcatatga tcggctcccg
     3001 catcggcgag ctcatcggcg aggcgcagct cgtcgtgaac tgggaggcct accccgaaga
     3061 cgtcgcgccg ttcatccacg cgcaccccac ccagggcgag tcgctgggcg aggcgttcct
     3121 tcacctgacg ggcaaggctc tgcacaccct gtaacgacgg cgacgtggcg accgtgcggc
     3181 ggccgtggtg aggcgatctt gccgcgcgac ccgcacgatt aggctgagtg tgagcacgca
     3241 tctaaggaga cgaaatgagt gaaaccgtca gcctcccggc gctcggcgag agcgtcacgg
     3301 agggtacggt cacccgctgg ttgaagaacg tcggcgaccg ggtcgaggtc gacgagcctc
     3361 tgctcgaggt ctccaccgac aaggtcgaca ccgaggtccc ttctccgatc tccggcgtcg
     3421 tggaagagat cctcgtcgcc gaggacgaga ccgttgacgt gggcaccgtg ctggtgcgta
     3481 tcggcgacgg cagtggccag gccgcggcgc aagcatctgc cgagcccgcc gccgcgccgg
     3541 aacgccaggc gcccgcccct cagccggcgg cagccgaacc ggctcccgtg gccgcggcgc
     3601 cgctcgcccc ggccgcaccc accgcagcgc ctgtgcagcc tacttacacg cctcccgccc
     3661 cggtcgccgc gccgcagccg accgcaccgc agcctgtggt ccaggctccg ctcccgccgc
     3721 gcgccgcagc acccgcaccc gcggcacagg cttccacccc cacccccgcc gcggcacagg
     3781 ccccggcgac gccgaccacc ggcgccccgg caccacgagg cgccgcacac gccggtcagt
     3841 acgtcacacc gatcgtgcga aagctcgcca gcgagcaagg ggtcgatctc gacaccgtcg
     3901 cgggcagcgg cgtcggtggg cgcatccgca aggaggacgt gctgcaggcc gcgacgcgtg
     3961 ccgaggtcgt agactccgga atccgcggaa cccgcgaagc gatgtcggcg ctgcgtcgcg
     4021 tgacggcgga gcgcgccgtc gcctcactcc gcgagtcggc acaagcgacc tgcgtcaaag
     4081 aggtcgacgt gactgacctg atgcgaacga tcgcggcctc ggcggcacaa ttcgaacgcg
     4141 cgaacacggt gccgttgggc ttcctcccgt tcgtcgtccg ggccaccgct gaggcgttgc
     4201 gacaacaccc gatggtgaac gcctccgtca cgggcgacac gatcgtctac cccgaagacg
     4261 agaacatctg cctcacggtg gacaccgata agggcatcct gacccccgtc ttgcgtggtg
     4321 ccgcgaacct ctcgatcgcg caggtggcga aggagatcgt ggaagtcacc ggcagggtcg
     4381 ccgcaaacac gctgcgcccg gacgagcttg tggggggcac cttcacgatc acggactccg
     4441 gcgcacgagg ggctctcttc gacaccccgg tggtgttcct tccccaagtg gcggcgctcg
     4501 gcgtcggcga ggtgacgaag cggcccgccg tggtctcggt agccggaaca gacgcgatcg
     4561 ccattcgatc gagcgtgttc ttctccctct cgtacgatca gcgcatcatt gacagcgcgc
     4621 aggccgcccg gttcctgaca acactggccg ggagtctcgg gtcggcggcc gtggacgggg
     4681 ttctctagcc cggtcacgca cgcccagcgc cgttagcgcg caccgttcag ccgaagactt
     4741 cgcggagggc ccagccgctc tccgtctcca tcaccaagac cgaaagtggc ggcgcgttct
     4801 tggtacctgc cgccgggtcg acggagacga gggcggcgcc gccgtttcgc tgcaccaggg
     4861 cggcctcacg cccggcgtat gtgacaggtt tgtgctccgc ggtgccggca agcacggcgg
     4921 catctgcccg ggcttgtggg gagccgggct cgtcgacgcg cgccaagcag gccgccgagc
     4981 cgctcgagaa gcacctctcc cgggcgagca ggagggcgac cgcggcgctg gccgggtccg
     5041 ctgcggcgcc cgcatcgcgc cccgacgggg tcgcggtgtc ttcggccgtg ccgccgacgc
     5101 ccgtcgcctc agacggcgaa ggtgacgtgg tgccagcggc accagcggca ccagaatcac
     5161 gatcggtggc gccgcgcccc gtcgaggcag cggcgtcacc gggggcgccg gtcgacggcg
     5221 ccggcgccga accgagcgga agcgccgcca gcgcggccgc gagagccacg gccccggcga
     5281 ctccggcaac gatcaacggg cgcctccgcc ggcgcaggcc acgaataagg ggctcgatga
     5341 cgtttctggc ctggtcgatg aggctgctac cccgtgtctc gaaggcgagt ggcctgccgt
     5401 atgccgacgg ctgagccgga gtgtcggggc cgaggtcgaa gccggttcct gtgtcggagt
     5461 cggcggtcgg agcagctcct ccctgcgcag cctccggctc ctcgacctgc gtcgtgagag
     5521 gttccggcgg cgcgtaggcg aagacggcgt cttccaacgc ttcgagcgcg ctgccggcat
     5581 cggacgtgct gccggggtcg tctgcgtcgg gggcatcgac accgtctgat cccccggggc
     5641 ccgcgacgag tagcccgtgc ggcggcgcct gccccggcag gcgctcgggg tgccaggcgt
     5701 gcagcacggc gccccgccat cggtctcgca tgccgggcgg caggcgcaac gcgacgctgt
     5761 gcagcaaggc gcgcacacct cggtcgaggc cgtcttcgac atcgtcgtcg aagacggccc
     5821 gagactcgtc gtcagtaccg ccagcgcccc ggcgcaccag cccaccgagg ccgacgagca
     5881 ccggccggcc gtcgccgtcg agacggaacc tgctgggtga ggggccgccg tcgctcgccc
     5941 acccggcggc ccgtaaggcg ccctccgcac gcaagacgct cacgacgacc gtggtggcct
     6001 caccgggcgt gacacgatgc ctgcggtcga gccattcggt caccgacggg ccggccaccc
     6061 actccagcac aagggcaccg tagccgtctg cgagccaggt gaagtcgcgc agcgcgacca
     6121 cgtgat
//
LOCUS       DASTNL010000183        14599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_876444, whole genome shotgun sequence.
ACCESSION   DASTNL010000183 DASTNL010000000
VERSION     DASTNL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876444"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFQ96_05605"
     CDS             <1..395
                     /locus_tag="VFQ96_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0257298.1"
                     /translation="MLDLIADIPDVFAVGGLTMGADPIATAILHQGAARGLAYDAFVV
                     RKKPKDHGRGRQVEGPDVAGRRVVVVEDTSTTGGSPLQAADALVKAGARIAAVAVVVD
                     RDTGARQAVEAAGYRYLAAIGLADLGLA"
     gene            509..1087
                     /locus_tag="VFQ96_05610"
     CDS             509..1087
                     /locus_tag="VFQ96_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEU0257299.1"
                     /translation="MSVVVGVALLALVGVFFLALRLASPAATHAAPVVVPTALPAPHV
                     TPLPTTTLTGGTARWSSLAGGECFSPFTSPWAETFTVVDCTAPHQAQLVYATVLPGTA
                     SEAYPGQKAVVADAQKLCGQNGIINLAAAKDDTGVQVQAAYPASEEQWQSGQRGVYCF
                     ATLSSGAVMTGSIAGLVAGPAPTAAAGAGATG"
     gene            complement(1084..1860)
                     /locus_tag="VFQ96_05615"
     CDS             complement(1084..1860)
                     /locus_tag="VFQ96_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEU0257300.1"
                     /translation="MTDRSQITCWLTDMDGVLVHEDTVLPGAAELTRQWHDSGTPFLV
                     LTNNSIFTPRDLSARLHRLGLDVPENSIWTSALATAAFCAAQMPGGSAFVIGEAGLTT
                     ALHEAGFILSERSPDYVIVGETRNYSFEAITKAIRLIDRGSRFIVTNPDATGPSAEGP
                     LPATGAIAALITKATNRTPYVVGKPNPMMFRSALNKIGAHSEHTAMIGDRMDTDIAAG
                     IEAGLYTILVLTGISGRADIARFPYRPAEVVEGVHELVEA"
     gene            1987..2652
                     /locus_tag="VFQ96_05620"
     CDS             1987..2652
                     /locus_tag="VFQ96_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU0257301.1"
                     /translation="MPPEPPHGGAAAERSLFGGEPRVYGAGPWQGPWPDDPRYDPELL
                     RDGDRRNVIDRFRYWTMEAIVEELDKARHPFHVAIENWQHDLNIGSIVRSANAFGAEA
                     VHIVGRRRWNKRGALVTDRYQHVYHHPDVPSLVAWAAGAGLPVIAVDNVAGSVPIETF
                     ALPRRCLMVFGQEGPGITDDTVRAADAIVAITQFGSTRSINASAAAAVAMHAWVLQHA
                     EIA"
     gene            2803..3543
                     /locus_tag="VFQ96_05625"
     CDS             2803..3543
                     /locus_tag="VFQ96_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0257302.1"
                     /translation="MMESPRRRTVVPASRPDPLRPVPHAGRALTLGIVGGLLVIALGL
                     VVTHAAGWTSDELRVLQWFSSHQVPALNTVALGIAWLFAPTEALALTVLGTLAVLAVS
                     RDPWRAGTFLLVVALSWGSSKVVKMIVARPRPDGALLAHPLAIEHSASFPSGHVCFAA
                     ALAIGILVTMRDTRRRSRVIAIVVAGVGVVIVALSRLYVGVHYPTDVIGAIVYVPSAA
                     AVLLWLWLDVVLPRLPRSTPGRRVAASR"
     gene            3623..4123
                     /locus_tag="VFQ96_05630"
     CDS             3623..4123
                     /locus_tag="VFQ96_05630"
                     /inference="COORDINATES: protein motif:HMM:NF022793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HEU0257303.1"
                     /translation="MAAGERGRQTAPAGEAHPVVLADEPAVREKVRAAGRAVQRLKAI
                     AVAHPTSVLPWLALADVTFRADSPAESYAYARVAYGRALAALTGAGWRIGRAVPWSSP
                     ANRPALRAVFVLRRAAALLGLAGEERQYTALLRDADPEALGHLDDESPVPPAPPTETI
                     VIRGLD"
     gene            4271..5560
                     /locus_tag="VFQ96_05635"
     CDS             4271..5560
                     /locus_tag="VFQ96_05635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU0257304.1"
                     /translation="MPASVIIGAQWGDEGKGKATDLLSNHIDYVVKFNGGNNAGHTVV
                     VGDEKYALHLLPSGILTPGVVPVIGNGVVVDLDVLFGELDALVERGVDVSRLRISANS
                     HVITLYHRTLDKVTERFLGKRRIGTTGRGIGPTYADKINRQGIRIQDLFDENILRQKV
                     AGALDQKNHLLVKVYNRRAITVDEIVESLLVHTERLRPLVADTSLLLNRALDEGKHVL
                     FEAGQATMLDIDHGTYPFVTSSNSTAGGAATGSGVGPTRITRVIAVVKAYTSRVGAGP
                     FPTELFDEKGDWLRERGFEYGTTTGRPRRTGWYDAVVARYATRVNAVTDFVLTKLDTL
                     TGLARIPVCVAYDVDGVRHDEMPVSQTDFHHAVPIYEELPGWSEDITGVRSFAALPKA
                     AQDYVVALEEMSGARISAIGVGPARDAIVVRHSLTDD"
     gene            complement(5749..6225)
                     /locus_tag="VFQ96_05640"
     CDS             complement(5749..6225)
                     /locus_tag="VFQ96_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIP domain-containing protein"
                     /protein_id="HEU0257305.1"
                     /translation="MTPRHHGPSEQVLIAGDEDDLPAIHHLLWLLPEDVYGHVFIETL
                     TEDGRTPLAAPERVSVTWLPRAARRSRLRSLVFADRGEPLAAALVGWAAEWLDGGCGQ
                     LADRHLLWVGAAGSGNVAATRDLVLEALCGHVYALGEAGGVRGTIPWAKGAPNTAA"
     gene            complement(6336..6824)
                     /locus_tag="VFQ96_05645"
     CDS             complement(6336..6824)
                     /locus_tag="VFQ96_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU0257306.1"
                     /translation="MSTTTRRAPGLSASPELAAGVAQFLAPVVIDMEALVVNGKQAHW
                     HVRGHNFIGIHELLDDVVEHAQGFADLAAERVVSLGLPVDSRLATVAQKSTLPQIPDG
                     FVDWDDTVAEVVGLIDAALVTVRTAVQGLDEVDLSSQDVAIAIEQGLTKDRWLLQAHI
                     AG"
     gene            7074..7385
                     /locus_tag="VFQ96_05650"
     CDS             7074..7385
                     /locus_tag="VFQ96_05650"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU0257307.1"
                     /translation="MTYDEAVARLRDIRSSIDNLDAALVRILAERFRCTQAVGELKAE
                     HGMPAADPERERVQIKRLRALAEESQLDPEFAEKWFNFVVAEVIHHHIAAAELRGAAK
                     G"
     gene            complement(7394..9721)
                     /gene="purL"
                     /locus_tag="VFQ96_05655"
     CDS             complement(7394..9721)
                     /gene="purL"
                     /locus_tag="VFQ96_05655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU0257308.1"
                     /translation="MSEGTASEYVPDTVADAIATPEKPQPYAALGLTDAEYAKIRQIL
                     GRRPTGGELAMYSVMWSEHCSYKSSKKYLRQFGQKVTPEMTRNLLVGMGENAGVVDIG
                     EGWAVTFKVESHNHPSFVEPFQGAATGVGGIVRDIIAMGARPVAVMDQLRFGAIDDPD
                     TARVVHGVVGGISFYGNCLGLPNIGGETYFDPIYRGNPLVNALAVGVLRHDDLHLANA
                     RGAGNKIVLFGARTGGDGIGGASILASEAFTAGGPTKRPAVQVGDPFAEKVLIECCLE
                     LFSGGLVEGIQDLGAAGISCATSELAAGGDGGMHIRLDEVLLRDPTLSAEEILMSESQ
                     ERMMAVVTPDRLERFLAVTSRWDVEASVLGEVTDTGRLVIDWHGETIVDVEPGTVAQG
                     GPVYDRPTARPSWLDALQADTADRLERTNEPDLLRDQFLRVVTSPNLADKSWVTNQYD
                     FYVGGNTALTFPDDGGMIRVDEASGLGVTIATDANGRYAQLDPREGARLSLAEAYRNV
                     AVTGAKPAAVTDCLNFGSPEDPEVMWQFSEAVAGLSDGCLELGIPVTGGNVSFYNQTE
                     GTPIHPTPVAGVLGVIDHVARRVPSAWQDRGENIYLLGVTRQELDGSAWAGTIHGHLG
                     GRPPAVEMATEKALAGLLHAAALEGLATSAHDLSDGGLAQALTEGVVRFGVGARVWLN
                     EIMERDSVDAATALFSESTGRVIVTVSRDDDVKFQGLCDGRGYPVLRIGVTDDGGWEG
                     AALEVQGLFTVGLAELRAAHEDTLPAHFGPTVPRG"
     gene            complement(9776..11143)
                     /locus_tag="VFQ96_05660"
     CDS             complement(9776..11143)
                     /locus_tag="VFQ96_05660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU0257309.1"
                     /translation="MIEITMPRLSDTMEEGAIANWHKKPGDTVEIGDILVEIETDKAT
                     MDYEAYEAGTLSKILAQEGDMVAIGQPIALLDNGTGDETPAPQAGEAEPATAAGGPSA
                     PEPAGTASAAAEPAAPEKAAPQAAPATVSAEVSQQQGAARRISSPLVRKIARENGLDL
                     THVQGTGPGGRIIRKDLDALLAEAQGRAASAATNAAAAAPTGPSIPARGGSAAPATSA
                     ADEKRGTHDVPMTNIRRVIARRLGESARTIPHFFVTASADAEEFMRLRGELNTKLLAA
                     GRPKVSVNDLLIRAVAIALREHPNVNASYVGDDSPTMQIHDRINIGVAVAAESGLVVP
                     VITDADQKTVTQLGVEAKKLIGLAGDKKLSPEQMSGGTFTISNLGMYGVEDFTAIINP
                     PEGAILAIGGTKKEPVVVGDEIVAHYVMRYTMSSDHRIIDGALAAQFLKTLTGLIENP
                     WTILA"
     gene            complement(11171..12166)
                     /locus_tag="VFQ96_05665"
     CDS             complement(11171..12166)
                     /locus_tag="VFQ96_05665"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU0257310.1"
                     /translation="MSIITYRQALHDTLRAEMLRDEKVFAIGEEIGIFEGSYKITAGL
                     LKEFGPKRVRDTPIAEEGFTGAAIGAAMLGLRPVVEIMTINFSLLALDQIVNAAAKIY
                     GMFGGQSKVPMVIRTPGGGGQQLGATHSQNIELYYAFVPGLKVVAPSTPADAKALLLA
                     AIRDDDPVLFLENLALYNTKGEVPDDDTPAQIGKAAVARPGKDLTLIGYSRTAYEARE
                     VAQRLAESDGLDIEVIDLRSLRPLDRPTIVESVKKTHAAVVLEDDWLTYGVGAEIAAT
                     ISDGAFDYLDAPVRRVAMAEVPLPYSKPLETAALPSEDDIIAAIRQTLDAVGRRR"
     gene            complement(12163..13197)
                     /gene="pdhA"
                     /locus_tag="VFQ96_05670"
     CDS             complement(12163..13197)
                     /gene="pdhA"
                     /locus_tag="VFQ96_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU0257311.1"
                     /translation="MVDDATLLEFYTQMMHIRRFEERAARAYTQALIGGYCHLNLGEE
                     AAIVGLLKALKKTDYLFTNYREHGYAITRGIHPNRVMAELYGRVDGVSKGWGGSMHMF
                     DLTARLLGGYGIVGGQLPLATGAGLAIDYRDSDEVVMVTMGDGTTNIGAFHESLNIAA
                     LWNLPVVYVIINNKLGMGTSVDKSSAEPELYKRASSYRMASAEVDGSDPTAVFDATTT
                     AVASARERKPFLLEVHTERLKGHSVVDPAKYRSAEENEELKENDPVLTFARRLLDDGV
                     LDADTKKKIDQEAATMASDAAAFAEKSPFPEVSTLFDYTYATPVPNDSRRLPGQPLFE
                     PLPIPTGAAR"
     gene            13181..13303
                     /locus_tag="VFQ96_05675"
     CDS             13181..13303
                     /locus_tag="VFQ96_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257312.1"
                     /translation="MASSTIDGAFADLLFLGVVPRCAVRTPVLERAGVALADLG"
     gene            13504..14124
                     /locus_tag="VFQ96_05680"
     CDS             13504..14124
                     /locus_tag="VFQ96_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0257313.1"
                     /translation="MPAASGDRGPRKRGRPTERERVLRRAAILEAALRVFLARGYGNA
                     TIEELASAGGVTKRTVYSYFGDKAGVFRDMVQSLAVTVSSSAPDQDTLESLAARIVYR
                     LYSAELIGLHRLVIAESSRFPELASALHRDGDIRHIARLADHLAAERGSETAGLAPAL
                     FSLLLGEEHRKRLLGLAPPMTEFEAWEHAAAALAALSLERAEVGRD"
     gene            complement(14127..>14599)
                     /locus_tag="VFQ96_05685"
     CDS             complement(14127..>14599)
                     /locus_tag="VFQ96_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257314.1"
                     /translation="TGVVTSRILEAAVVLTGAVFFGTLTTALVSGRAAAWTTSIVLFA
                     ASLLALAMAPVRLTVDSRGIRLVSVVLRVPLLRLHLANMESVTADTIDPMRWGGWGYR
                     VSGAGIAYVARRGPGIVIERRRGAAVAITLDHPEEAAALANGLIAARPAPGPGR"
BASE COUNT         2106 a         5020 c         5121 g         2352 t
ORIGIN      
        1 tgatgctcga ccttatcgcc gacatccccg acgtctttgc cgtcggcggc ctgacgatgg
       61 gcgccgaccc gatcgcgacg gcgatcctcc accagggggc tgcccgcggg ctggcgtacg
      121 acgcgttcgt cgtgcgcaag aagccgaagg atcacggccg cggccggcag gtcgaggggc
      181 cggatgtcgc ggggcggcgc gtcgtcgtcg tggaagacac ctccacgacc ggcgggtcgc
      241 cgttgcaggc ggccgacgca ctcgtcaaag ccggcgcccg gatcgccgcc gtcgccgtcg
      301 tggtcgaccg cgacaccggc gcccggcagg ccgtcgaggc cgccggttac cgttacctcg
      361 ccgcgatcgg attggccgat ctgggactgg cctagtggcg cccggcgacg agccgacggg
      421 ggagccgacc gcgagctggc cgttcccaga gcttgtcgag cccgccaggc agaccgcggc
      481 cgggcctact cgccgcgaga tacgtgtggt gtcggtcgtt gtcggcgtgg cgctcctcgc
      541 gctcgtcggc gtgttctttc tcgccttgcg tctcgcctcg cctgccgcga cgcacgccgc
      601 cccggtcgtg gtccccacag cgttgcccgc cccgcacgtg acaccgctgc ccacgacgac
      661 cctcacgggg ggcaccgccc gctggagctc cctggcaggc ggagaatgct tcagcccgtt
      721 cacctcgccg tgggccgaga cgttcacggt cgtcgactgc accgcccccc accaggcgca
      781 gctggtctat gcgaccgtgc tccccggcac cgcgtcagag gcgtaccccg gacagaaggc
      841 ggtggtggcc gacgcgcaga agctctgcgg ccagaacggc atcatcaacc tggcggcggc
      901 gaaagacgac accggagtgc aggtgcaggc cgcgtacccg gcgagcgagg agcagtggca
      961 gtcggggcaa cgcggcgtct actgcttcgc cacgctctcc tccggcgccg tcatgacggg
     1021 cagcatcgcc ggtctggtcg cgggcccggc gccgaccgcg gcggccggcg ccggcgcgac
     1081 gggctaagcc tcgaccagct cgtgcacacc ctcgacgacc tccgccgggc ggtaggggaa
     1141 ccgcgcgatg tctgcccgcc cgctgatgcc ggtcaacacg agaatcgtgt acaggcccgc
     1201 ctcgatgcct gcggcgatgt cggtgtccat ccggtcgccg atcatcgccg tgtgctcgga
     1261 gtgcgcgccg atcttgttca gggcggaacg gaacatcatc gggttcggct tgccgacgac
     1321 gtacggcgtg cgattcgtgg ccttcgtgat gagcgccgcg atcgcgcccg tcgcgggcag
     1381 cgggccctcg gcgctgggcc cggtcgcgtc cgggttcgtc acgatgaacc gggaccctcg
     1441 gtcgatcagc cggatcgcct tcgtgatcgc ctcgaacgag tagttgcggg tctctcccac
     1501 gatcacgtaa tcgggcgatc gctcactgag gatgaatcct gcttcgtgca gggccgtcgt
     1561 gaggcccgcc tcgccgatca cgaaggcgct gccgcccggc atctgggccg cacagaaggc
     1621 ggcggtggcg agggctgagg tccagatgga gttctccggc acgtcgagcc ccagcctgtg
     1681 cagccgggcg ctcaggtcac gtggcgtgaa gatcgagttg ttcgtcagca ccagaaacgg
     1741 cgtgcccgag tcgtgccact gcctcgtgag ctccgccgcg ccgggcagga cggtgtcctc
     1801 gtgcacgagt acgccatcca tgtcggtcag ccaacaggtg atctgggaac gatcggtcat
     1861 gggacctcct ctcggcatca tcgtagggcc tgggtgcgcc gaacgtgccg cgcccgcgcc
     1921 tgggttcggg gtgcgcctga ggcgccccag cggtctcccg gccggtaacg tcagagggtg
     1981 aacaccgtgc ctcccgaacc gccccatggc ggcgccgcgg ccgaacggag cctcttcggc
     2041 ggcgagccgc gcgtttacgg cgcggggccg tggcaggggc cctggcccga cgaccctcgc
     2101 tacgaccccg agctgctgag agacggcgac cgccgcaacg ttatcgaccg gtttcgctat
     2161 tggacgatgg aggcgatcgt cgaagagctc gacaaggcgc ggcatccctt ccacgtggcc
     2221 atcgagaatt ggcaacacga cctgaatatc ggctcgatcg tgcgcagcgc caacgcgttc
     2281 ggcgccgagg cggtgcatat cgtgggccgg cgccggtgga acaagcgcgg cgcactcgtc
     2341 accgaccgct atcagcacgt gtatcaccac ccggatgtgc cgtccctcgt cgcctgggcc
     2401 gccggggccg ggctgccggt gatcgccgtg gacaacgtgg ccggcagcgt gccgatcgag
     2461 acgttcgcgc tgccgaggcg ctgcctcatg gtcttcggcc aggagggccc cggtataacc
     2521 gacgacacgg tgcgggctgc cgacgcgatc gtcgccatca cgcagttcgg ctccacgcgt
     2581 tccatcaacg cctccgcggc ggccgccgtc gccatgcacg cctgggtcct gcagcacgcc
     2641 gagatcgcct gaccgcacgg ctcgcggctc acgctccgcg gccgaggcat gggccggccg
     2701 ccgactctgt gccgggtggg ggccgccgtg tcagcgtgcg gtcggcaaac gcccgtagac
     2761 tctgctgcgg cgccggccgg cggccaccac ggaggacaca cgatgatgga atcacccagg
     2821 cgccgaacgg tcgtcccggc gagccgcccc gacccgttgc ggccggttcc gcacgcgggc
     2881 agggcgttga ctctcggcat cgtgggcggc ctcctcgtca tcgcgctcgg gttggtcgtc
     2941 acgcacgcgg cgggctggac gagcgacgag ctgcgggttc tgcaatggtt cagcagccac
     3001 caggtgccgg cgctcaacac ggtcgctctg ggtatcgcct ggctgttcgc gcccacggag
     3061 gcgctggcgc tgaccgtgct cggcaccctc gcggtgttgg ccgtaagccg cgacccgtgg
     3121 cgggccggaa cgttccttct tgtcgtcgcg ttgagctggg gcagcagcaa ggtcgtgaag
     3181 atgatcgttg ccaggccccg gcccgacggc gcccttttgg cccacccgct cgccatcgag
     3241 cactccgcca gcttcccgag cgggcacgtg tgcttcgcgg cggcattggc catagggatc
     3301 ctcgtgacca tgcgcgacac ccggcggcgc agccgcgtga tcgcgatcgt cgtcgccggc
     3361 gtgggcgtcg tgatcgtcgc cctctcccgg ctctatgtcg gcgtgcacta cccaaccgac
     3421 gtcatcggcg cgatcgtgta cgtgccgtcg gccgccgccg tgctgttgtg gctctggctc
     3481 gacgtggtcc tgccccgcct gccgcgaagc accccggggc gccgggtcgc ggcctcccgc
     3541 tagagcgccg gagcccggcg aacggggccg gcccgcgccg aagagacacc gcacggtcag
     3601 gaacactcgt tagcatcgag gcatggctgc aggggagcgg ggccggcaga cggcgccggc
     3661 cggcgaggcg cacccggtcg tgttggccga cgagccggcc gtgcgggaga aggttcgggc
     3721 ggcgggccga gcggtgcagc gcctgaaggc gatcgcggtg gcgcacccaa cctcggtgct
     3781 gccgtggctc gcgttggccg acgtgacgtt ccgggccgat tccccggcgg agtcgtacgc
     3841 gtacgcgcgg gtcgcctacg ggcgggcgct ggcggccctg acgggagccg ggtggcgcat
     3901 cggccgggcc gtcccctggt cgtccccggc caaccgcccg gcgttgcgcg ccgtctttgt
     3961 gctgcgccgt gccgcggcac ttctggggct tgccggcgag gaacggcagt acacggcgct
     4021 cttgcgggac gccgaccccg aggctctggg gcatcttgac gacgagtcgc ccgtgccgcc
     4081 ggcgccgccc acggagacga tcgtcatccg cgggctcgac tgagcgcggc gggcctcctg
     4141 ggcgccggcg ccatctcggc tcgtcgggtg gccccggtag gatgggacgc gcggcgtcga
     4201 aggcccgtgc ttcggcacca ggcagcagcc tcccggccgc gcatttccga gcgcgaggag
     4261 agcagcaacc atgccggcaa gcgtgatcat cggcgcccaa tggggcgacg agggcaaagg
     4321 caaggccacc gatctgctca gcaaccacat cgactacgtc gtgaagttca acggcggcaa
     4381 caacgcaggc cacaccgtcg tcgtcggcga cgagaaatac gccctgcacc tcttgccctc
     4441 cggcatcctg acgcccggcg tcgtgcctgt catcggcaac ggcgtcgtcg tcgacctcga
     4501 cgtgctcttc ggcgagctcg atgcccttgt ggagcgcggc gtcgacgtct cgcggctgcg
     4561 catctctgcg aactcccacg tcataacgct ctaccaccgc accctcgaca aggtcaccga
     4621 gcgtttcctc ggcaagcgcc gcatcggcac gaccggccgc ggcatcggcc cgacctacgc
     4681 cgacaagatc aaccggcagg gcatccgcat ccaagacctc ttcgacgaga acatccttcg
     4741 gcagaaggtc gccggtgcgc tcgatcagaa gaaccacctg ctcgtcaagg tctacaaccg
     4801 gcgggccatc acggtcgacg agatcgtcga gagcctgctc gtgcacaccg agcggttgag
     4861 gcccctcgtc gccgacacga gcctgctgct caaccgggcg ctcgacgagg gcaagcacgt
     4921 gctgttcgag gcgggccagg ccacgatgct cgacatcgac cacggcacct acccgttcgt
     4981 cacctcctcc aactcgaccg ccggcggggc ggcgaccggg tccggtgtgg ggcccacccg
     5041 catcacccgg gtcatcgccg tcgtgaaggc ctacaccagc cgtgtcggcg ccggcccgtt
     5101 cccgaccgag ctcttcgacg agaagggcga ctggctgcga gaacgcggct tcgaatacgg
     5161 cacgacgacc ggccggccgc gccgcacggg ctggtacgac gccgtcgtcg cccgctacgc
     5221 cacacgggtg aacgccgtga ccgacttcgt gttgacgaag ctcgacacgt tgaccgggct
     5281 cgcgcgcatc ccggtctgcg tcgcctacga cgtggatggc gtgcggcacg acgagatgcc
     5341 ggtctcccag accgacttcc accacgccgt gcccatctac gaggagctgc cgggctggtc
     5401 agaagacatc acgggcgtgc ggtcgtttgc tgcgctgccg aaggcggcac aagactacgt
     5461 ggtggcgctc gaagagatga gcggcgcccg gatctccgcc atcggcgtcg ggcccgcccg
     5521 cgacgcgatc gtcgtgcgtc acagcctcac cgacgactga cctgtcgacg ggcgatcggc
     5581 ctggagggcc gggctcaccg atcagtcgac ccgcgaggcc ggacctcgcc tcgaggccgg
     5641 gtgccgggcg atcggcccgc gaagggccgg gctcgcctcg aggctgagcg cccagggcca
     5701 ccggccctca gacggccggc attgtcgccc ggccccattg atcggcgttc aggcggcggt
     5761 gttcggggcg cccttcgccc agggtatggt gccccgcacg ccccctgcct caccgagtgc
     5821 gtagacgtgg ccgcacagcg cttcgagaac gaggtctcgg gtcgcggcca cgtttccgct
     5881 gccggccgca ccgacccaca agagatgccg gtcggccagc tggccacagc cgccgtcgag
     5941 ccactcggcg gcccagccga ccaaggcggc ggcgagcggt tcgccgcggt cggcgaagac
     6001 cagcgagcgc aggcggcttc gccgcgcggc gcgcggcagc cacgtgacgc tcacccgttc
     6061 cggcgcggcg agcggcgtcc ggccatcctc cgtcaacgtc tcgatgaaca cgtgcccgta
     6121 gacgtcttcg ggcagcagcc acaacaggtg gtggatggcc ggcaggtcgt cttcgtcgcc
     6181 cgcgatgagg acctgttcag aggggccgtg gtgccgaggc gtcatgaggt aagtataggt
     6241 aagccttacc tacttcggaa ggggtcgagg cgtcgcacga agaatcaccc gccgcagcgc
     6301 actcggaagc gcgccggcgg gtggccgcat tcccgtcagc cggcgatgtg ggcctggagc
     6361 agccagcggt ctttggtgag gccctgctcg atggcgatcg cgacatcctg gctgctgaga
     6421 tcgacctcgt cgagcccctg aaccgccgtg cgcacggtca cgagcgcggc atcgatgagg
     6481 ccgacgacct cggcgacggt gtcgtcccag tcgacgaaac cgtcgggaat ctgcggcaac
     6541 gtgctctttt gcgccaccgt cgccagtcgg gagtcgacgg gcagccccaa cgagacgaca
     6601 cgttccgcag cgaggtcagc gaacccttgc gcgtgctcga ccacgtcgtc taacagttcg
     6661 tggatgccga tgaagttgtg gccgcgcacg tgccagtgcg cctgcttgcc gttcacgacg
     6721 agtgcttcca tgtcgatcac gacgggagcg aggaattggg cgacgccggc ggcgagctcc
     6781 ggggaggcgg agaggccggg ggcgcgtcga gtcgtggtac tcatgatgtc tggtcccttc
     6841 gttgtcgagt aagcatctac acccttcaac actcggcccg aatcgatgat tccgcaaccc
     6901 agcgaaggct ggcctcgtcg ggcgccatgg ggatctcgat agaatcgtcg cacctgccgc
     6961 tacggcggcg agagaccggc cccgcggcag ggccttagcc ggcagacggt gcgggtctgg
     7021 ggcgcggtgc gacggcgggc gtgaggccca gccgaagaga cgggggacgg cggatgacgt
     7081 acgacgaagc ggtggccagg ctgcgcgaca tccgcagcag catcgacaac ctcgacgccg
     7141 cgctcgtgcg tattcttgcc gaacggttcc ggtgcacgca ggccgtcggg gagctgaagg
     7201 cggaacacgg catgccggcg gccgaccccg agcgggagcg ggtgcagatc aagcgtctgc
     7261 gcgcgctcgc cgaagagagc cagctcgacc ccgagttcgc cgagaagtgg ttcaacttcg
     7321 tcgtggcaga ggtcatccac catcacatcg cggccgccga gttgcggggc gccgccaaag
     7381 ggtagccgct tggtcagccg cgcggcacgg tcggcccgaa atgcgccggc aaggtgtctt
     7441 cgtgggcggc gcgcagctcg gccaggccga cggtgaagag gccctgtacc tcgagcgccg
     7501 cgccctccca gcctccgtcg tcggtcacgc cgatgcgcag caccgggtag cccctgccgt
     7561 cgcacaggcc ctggaacttg acgtcgtcgt cgcgggagac cgtcacgatc acccgtccgg
     7621 tcgactccga gaaaagagcc gtcgcggcat ccacgctgtc gcgttccatg atctcgttca
     7681 accagacgcg tgcacccacg ccgaagcgca cgacgccttc ggtcagggcc tgcgcgagcc
     7741 ccccgtcgga gaggtcgtgc gccgaggtgg ccagcccctc cagcgcggcg gcgtgcagca
     7801 agcctgcgag ggctttctct gtggccatct cgacggccgg cgggcggccg ccgagatggc
     7861 cgtgtatggt gccggcccat gcggagccgt cgagctcctg ccgggtgacg cccagcaggt
     7921 agatgttctc gccgcggtct tgccaggcgc taggaacccg acgcgccacg tggtcgatga
     7981 cgcccagaac gccggctacc ggagtcgggt ggatgggcgt tccttcggtc tggttgtaga
     8041 acgagacgtt gccgcccgtg acggggatgc cgagttccag acagccgtcg gagagacccg
     8101 cgacggcctc cgagaattgc cacatcacct ccgggtcttc tggtgagccg aagttcaggc
     8161 agtccgtgac ggcggcaggc ttcgccccgg tgacggcgac gttgcggtac gcctcggcca
     8221 gcgagagccg ggcaccctct cgggggtcta gttgtgcgta acggccgttg gcgtcggtgg
     8281 cgatggtgac gccgagtccg ctcgcctcgt cgacgcggat catgcccccg tcgtcgggga
     8341 aggtcagcgc cgtgttgccg cccacgtaga agtcgtactg gttcgtcacc cagcttttgt
     8401 cggccaggtt cgggctcgtg acgacgcgca agaactggtc gcgcagaagg tcgggctcat
     8461 tggtgcgctc caggcggtcg gccgtgtcgg cctgcaaggc gtcgagccag gagggccggg
     8521 cggtcggccg gtcgtacacg ggcccgccct gggcgaccgt gccgggctcc acgtcgacga
     8581 tcgtctcgcc gtgccagtcg atcacgagcc ggcccgtgtc ggtcacctcg ccgagaacgc
     8641 tcgcctccac gtcccaccgg ctcgtgacgg cgaggaagcg ctcgagacgg tcgggggtga
     8701 cgacggccat catccgctcc tggctctccg acatgaggat ctcttcggcg ctgagggtgg
     8761 ggtcgcgcag cagcacctcg tcgaggcgga tatgcatgcc gccgtcgccg cccgccgcca
     8821 actcgctggt cgcgcacgag atgccggccg caccgagatc ttggatgccc tcgacgagcc
     8881 cgccggagaa cagctcgagg cagcattcga tcagcacttt ctcggcgaac gggtcaccca
     8941 cctgcacggc ggggcgcttc gtcggcccgc ccgcggtgaa ggcctcggag gcgaggatgg
     9001 aggcaccgcc gataccgtcg ccgcccgtgc gcgctccgaa gagcacgatc ttgttgccgg
     9061 ccccgcgggc gttcgccagg tgcagatcgt cgtgccgcaa caccccgacc gcgagagcgt
     9121 tgacgagcgg gttgccgcgg tatatcgggt cgaaataggt ctcgccgccg atgttcggca
     9181 gccccaggca gttgccgtag aacgagattc cgccgaccac cccgtgcacg acccgagcgg
     9241 tgtcggggtc gtcgatcgcg ccgaagcgca gctggtccat gacggcgacc ggccgggcac
     9301 ccatcgcgat gatgtcgcgt acgatgccgc cgacgccggt cgccgcgcct tggaacggct
     9361 ccacgaagct cggatggttg tggctctcca ccttgaacgt gaccgcccaa ccctcgccga
     9421 tgtcgacgac gccggcgttc tcgcccatgc cgacgagaag gttgcgcgtc atctcgggcg
     9481 tgaccttctg cccgaactgg cgcaggtact tcttgctcga cttataggag cagtgctcgc
     9541 tccacatgac ggagtacatg gcgagctcgc cgccggtcgg gcgccggccg aggatctgtc
     9601 ggatcttcgc gtactccgcg tcggtgaggc cgagagccgc gtacggttgc ggcttctccg
     9661 gcgtcgcgat cgcgtcggcc acggtatcgg ggacgtactc ggatgccgtg ccttcgctca
     9721 cgtgcgccta ctccttgact gggttatgcg gacgtgccga ttctaccggc ggcgctcagg
     9781 cgaggatcgt ccaggggttc tcgatcaggc ccgtgagggt cttcaagaac tgggcggcca
     9841 gggcgccgtc gatgatccgg tggtcggaag acatcgtgta acgcatcacg tagtgcgcga
     9901 cgatctcgtc gccgaccacg accggctcct tcttggtgcc tccgatcgcg aggatcgccc
     9961 cctccggcgg gttgatgatc gccgtgaagt cttctacccc gtacatgccg aggttgctga
    10021 tcgtgaacgt tccgcccgac atctgctcgg ggctgagctt cttgtcgccg gcgagaccga
    10081 tgagcttctt cgcctccacc ccgagctggg tgaccgtctt ctggtcggcg tcggtgatga
    10141 cgggcacgac gagaccggat tcggccgcga cggcgacgcc gatgttgatc ctgtcgtgga
    10201 tctgcatcgt cggcgaatcg tcgcccacgt acgaggcgtt gacgttcgga tgctcgcgca
    10261 gcgcgatggc cacggcgcgg atgagcaggt cgttcacgct caccttgggc cggcccgcgg
    10321 cgagaagctt cgtgttgagc tcgccgcgca gccgcatgaa ctcctccgcg tcggccgagg
    10381 ccgtcacgaa gaagtggggg atcgtgcggg cactttcgcc cagccggcgc gcgatcacgc
    10441 ggcggatgtt cgtcatcggc acgtcgtgag tgccgcgctt ctcgtcggcg gcgcttgtcg
    10501 ccggtgccgc cgacccgccg cgcgccggga tgctcgggcc ggtgggtgcg gctgcggcgg
    10561 cgttcgtcgc agcagaggcg gcccggccct gcgcctcggc cagcagcgcg tcgaggtctt
    10621 tgcggatgat gcgcccgcct gggccggtgc cctggacatg ggtcagatcc agcccgttct
    10681 cccgggcgat cttgcgcacg agtggggagg agatccggcg ggccgcaccc tgctgctgcg
    10741 acacctcagc ggagaccgtg gccggtgcgg cctgcggcgc agctttctcg ggcgccgccg
    10801 gttccgctgc cgcagaggca gtgccggcag gctccggcgc cgaagggccg cctgccgccg
    10861 tcgccggctc ggcctcgccg gcctgtggcg caggtgtttc gtcgcccgtg ccgttgtcga
    10921 gcagcgcgat cggctggccg atcgcgacca tgtcgccctc ctgggcgagg atcttcgaga
    10981 gcgtgcctgc ctcgtacgcc tcgtagtcca tcgtcgcctt gtcggtctcg atctcgacga
    11041 ggatgtcgcc gatctcgacc gtgtcgcccg gtttcttgtg ccagttggcg atcgcgccct
    11101 cttccatcgt gtcggagagc cgcggcatgg tgatttcgat catgattctt cgcccgttat
    11161 ctcgctcgta ctacctgcgg cgtcccaccg cgtcaagggt ctgccgtatg gcggcgatga
    11221 tgtcgtcttc tgacggcagc gccgcggtct ccagcggctt cgaatacggc agcggaacct
    11281 ccgccatcgc gacgcgtcgc acgggggcgt cgaggtagtc gaacgccccg tcggagatcg
    11341 tcgcggcgat ctcggcgccg acgccgtagg tgagccagtc gtcttcgagc acgaccgcgg
    11401 cgtgcgtctt cttcaccgat tccacgatcg tcggccggtc taacgggcgc aggctccgca
    11461 ggtcgatcac ctcgatgtcg aggccgtcgc tctcggcgag gcgctgagcc acctcgcggg
    11521 cctcataggc cgtgcgggag tagccgatga gcgtcaggtc tttgccgggc cttgccacgg
    11581 ccgccttgcc gatctgcgcc ggtgtgtcgt cgtcgggcac ctcgcccttc gtgttgtata
    11641 gggccaggtt ctcgaggaag agcaccgggt cgtcgtcgcg gatggcggcg agcaacagcg
    11701 ctttcgcgtc cgccggcgtt gatggcgcga cgaccttcag gccgggcacg aatgcgtaat
    11761 acaactcgat gttctgtgag tgggtggcgc cgagttgctg gccgccgccg ccgggggtgc
    11821 ggatgaccat gggcaccttc gactgcccgc cgaacattcc gtagatcttc gccgcggcgt
    11881 tgacgatctg atcgagcgcc aggagcgaga agttgatggt catgatctcg acgaccgggc
    11941 gaagaccgag catcgcggca ccgatggccg cgccggtgaa cccctcttcg gcgatcggcg
    12001 tgtcgcgcac ccgtttcggc ccgaactcct tcaagagccc cgccgtgatc ttgtaggagc
    12061 cttcgaagat gccgatctcc tcgccgatcg cgaacacctt ctcgtcgcgc agcatctcgg
    12121 cgcgcaacgt gtcgtgcagc gcctgacggt aggtgatgat gctcatcggg cggctcccgt
    12181 cgggatcggc aacggctcga acagcggctg gcccggcagc cgccgggagt cgttcggcac
    12241 cggtgtcgcg taggtgtaat cgaacagggt ggagacctcg gggaacgggc tcttctcggc
    12301 gaacgcggcc gcgtcggagg ccatggtcgc cgcctcttga tcgatcttct tcttcgtgtc
    12361 ggcgtcgagc acgccgtcgt cgaggaggcg cctcgcaaac gtcagcaccg ggtcgttctc
    12421 tttcaactct tcgttctctt ccgccgaccg atacttggcg gggtcgacga ccgagtgccc
    12481 cttgagacgc tcggtatgca cctcgagaag gaacggcttg cgctcgcgcg ccgaggcgac
    12541 tgccgtcgtc gtcgcgtcga agaccgcggt cgggtcggag ccgtcaacct cggccgaggc
    12601 catccggtaa gaggaggcgc gcttgtagag ctcgggctcc gccgacgact tgtcgaccga
    12661 ggtgcccatg cccagcttgt tgttgatgat cacgtaaacg accggcaggt tccagagcgc
    12721 cgcgatgttc aacgattcgt ggaaggcgcc gatgttcgtg gtgccgtcgc ccatcgtgac
    12781 catgacgacc tcgtcgctgt cgcgatagtc gatggcgagg cccgcgccgg tggccagagg
    12841 cagctgcccg ccgacaatgc cgtagccgcc caagagcctc gcggtcaggt cgaacatgtg
    12901 catcgacccg ccccagccct tggagacgcc gtcgaccctg ccgtacagct ctgccatgac
    12961 gcggttcggg tggatgcctc gggtgatggc gtaaccgtgc tcccggtagt tggtgaagag
    13021 gtagtcggtc ttcttcaatg ccttcaggag cccgacgatc gcagcctctt cgccgaggtt
    13081 caggtggcag tagccgccga tgagcgcctg ggtgtaggcg cgggcggcac gctcctcgaa
    13141 gcgtcggatg tgcatcatct gggtgtagaa ctcgaggagc gtggcgtcgt cgaccatcga
    13201 cggcgccttc gcagatctct tgttcttggg ggtcgtgccc cgctgcgccg tgcggactcc
    13261 cgtcttggaa cgggcgggcg tcgcgctggc ggaccttggc tgagcggcca tgccggaagc
    13321 tcccttcgct gaccctcatg gggtcgcacg ggtgacgtga tgtgtcactg tgttaaagga
    13381 cgttggtctc agaatgaaac catcacgtat cattgtatat cagtggccgt gggtgcggca
    13441 ttcacattct ggcaccctcc gggcgtcacg gccgggggcg cccggccggc gtgaggagga
    13501 gcgatgcctg cagcatccgg cgaccggggg ccgcgcaagc gaggcaggcc caccgagcgc
    13561 gagcgcgtgc tgcgccgcgc ggccattctc gaggcggcgc tgcgtgtctt cttggcgcgt
    13621 ggctacggca acgcgacgat cgaggagctc gccagcgcgg ggggcgtgac caagcgcacc
    13681 gtctacagct acttcggcga caaggccggt gtctttcgcg acatggtgca gagccttgcc
    13741 gtgacggtat cgtcctcggc gcccgaccag gacacgttgg agagccttgc ggcccggatc
    13801 gtctaccgcc tctattccgc ggaactgatt ggcctccacc ggctcgtcat cgccgagtcg
    13861 agccggttcc ccgagctggc ctccgccctg catcgtgacg gcgatatccg ccacatcgcc
    13921 cgcctggccg accatctcgc cgccgagcgg gggtctgaga cggcgggcct cgccccggcg
    13981 cttttcagcc tgctgttggg ggaggaacat cgtaagaggc tcctgggtct ggcaccgccg
    14041 atgacggagt tcgaggcatg ggaacacgcg gcggcggccc tggccgcgct cagcctggag
    14101 cgcgccgagg tgggccgaga ctagcactag cgcccggggc cgggcgccgg ccgggcagcg
    14161 atcagcccgt tcgcgagagc ggcggcctct tcggggtggt cgagtgttat tgcgaccgcg
    14221 gcgccgcgtc gtcgctcgat gacgatgccg gggccgcggc gggcgacgta ggcgatcccc
    14281 gcgccgctca cgcggtagcc ccagccaccc cagcgcatcg ggtcgatggt gtccgccgtg
    14341 acggattcca tgttcgcgag gtggaggcgc aagagcggca cgcgcagcac cacagagacg
    14401 aggcggatgc cgcggctgtc gacggtcagc cgcacgggcg ccatcgcgag ggccagcagg
    14461 ctggccgcaa agaggacgat gctggtggtc cacgcagctg cgcggccgct gacgagcgcc
    14521 gtcgtgaggg tgccgaagaa gacggcgccg gtcaacacca ccgcagcctc gaggatccgg
    14581 cttgtcacga ctccggtcc
//
LOCUS       DASTNL010000184         8119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_137081, whole genome shotgun sequence.
ACCESSION   DASTNL010000184 DASTNL010000000
VERSION     DASTNL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137081"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFQ96_05690"
     CDS             <1..1010
                     /locus_tag="VFQ96_05690"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEU0257315.1"
                     /translation="TATTLRSVAGAALRGMLAEALPELDRTRELRARSRGRGRDLMAE
                     VLRRAVERGEIPPEVVLPGRLGVGGALLRDHYLFHNEPLDDTVLTRVVDDVLLPLFVA
                     SPPARASRSALETSAMAQNGARILRNQTVRELANDLRAQVARAPGQRWMLGVTGAPGA
                     GKSTLAGSLLAELGPDLAAVVPMDGFHLARAVIEGTALARRRGAPDTFDADGYVALLA
                     RLRRRDAAVVYAPAFRRGLEDPMAAAIAIPRHVPVVITEGNYLLCHDRSWAAVRTLLD
                     EVWYIEARGQTRTARLVARHTAFGMTPEQASAWAAETDAANARLVESTRGAADRVVLW
                     E"
     gene            complement(1118..1507)
                     /locus_tag="VFQ96_05695"
     CDS             complement(1118..1507)
                     /locus_tag="VFQ96_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEU0257316.1"
                     /translation="MAFVPGVAAVIVLTLVRVLLVVAFVREAVLKFTDVRGFAKNDGV
                     PLPVAWFVPIVEAAAALGLATGILATWAALGVILLMLVTIGMHVFRWHSTYWAQKGGP
                     EYDLMLLGLAAVIAVFGPGPVAIPFLT"
     gene            1612..2847
                     /locus_tag="VFQ96_05700"
     CDS             1612..2847
                     /locus_tag="VFQ96_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEU0257317.1"
                     /translation="MKLLSKLCVVAASVVVAAGLAAAPATAKTAPRQAFSDVPASSQF
                     AAAIDWLAQKHITTGYADDTFRPTRTITREAMAAFLYRLDGSPAEAAPASAPFPDVPR
                     DGHFAKEIAWLAGTHITTGFADGGFHPTAAITREAMAAFLYRYAAMKGTGDTASYQEP
                     ATSSFLDVAHSAQFSLEVSWLASTGITTGWQVGRGCEQFRPTASITREAMAAFLYRYA
                     TAGAKPGTAAPVTCDAPPAGEGSFSGDAGTLKVGTAVQPGTYVSSTPSSGPGCSWARL
                     SAAGDDAADTLASDLGAPGSQVIVTIDPADAYFATSGCGTWKALTGPTAQKPLTTIPG
                     DGGTYVVGWGVRPGTYASTTPASGPVCFYRRLSSADGSVAAILDDEVAVPGERVVVTI
                     EPGDLYFHTEGCGTWQALG"
     gene            3203..3829
                     /locus_tag="VFQ96_05705"
     CDS             3203..3829
                     /locus_tag="VFQ96_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU0257318.1"
                     /translation="MPEFQRPRRVPSFVFDALPGAHDPAESTALAHRTAAALVSRVRA
                     DPRPPVLDRLVAFADEHGLDAVAELWSAARPCSLPGALWRIYLLHAVAVRSAEECSYL
                     YRRGMESARGIDPVVAGAAVPTGPRELVALTTLILKGLFEGDFAVALERAAAYCRVVS
                     VGCASLADDFDGTEPERSSRLTRRGLRYHTISEDLTGCAALWRQGALE"
     gene            complement(4071..5006)
                     /locus_tag="VFQ96_05710"
     CDS             complement(4071..5006)
                     /locus_tag="VFQ96_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0257319.1"
                     /translation="MKQVFAAGAVCWRPREGKTPAILVVHRAKYGDVSLPKGKVEPGE
                     ALPQTAVREIREETGLAVVLGVPLGTTSYTIGGGRTKNVQYWAAEVAEEALARSTFVP
                     NHEIAALEWVGLKKARKYLSYERDVAIVDTFAALVERGVTSTFALIGLRHGQAVPATE
                     WDGPDATRPLTDHGARQASANVGPIGAWRPRKLLTSTAVRCVGTVAPLAASFGVRAVR
                     EEGLSQDAHEAGEGTVRQIVGKRVRKRRTAVLCSHGPVLPVILREIALAAGGEAPSEL
                     RDAARLTTGGFSVVHLSVSNPGAGILAIETHPEHR"
     gene            complement(5003..7180)
                     /locus_tag="VFQ96_05715"
     CDS             complement(5003..7180)
                     /locus_tag="VFQ96_05715"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEU0257320.1"
                     /translation="MDAENLLVGAEANSDLNDDFTSGPIEKTGTLPDDRYLNRELSWL
                     AFNLRVLELAEDPSIPVLERVNFLAIFASNLDEFFMVRVAGLKRRILTGLAVPTNTGQ
                     APLDVLADISRKAHELQDRHARALHDAVLPELAEAGITLVSWADLDEADRQQMHVLFN
                     TRIFPVLMPLAVDPAHPFPYISGLSLNLSVRVRNPKTGKEQFARLKVPQMMPRLLRVD
                     NREPLTRMRFIALEDLIAVHLDDLFAGMEVLDHHVFRVTRNEDVEVDEDETENLLQAL
                     EKELLRRRFGPPVRLEVTADMDEVTLGLLVRELDVSEQEVYRIPGLLDLGGLFDLAHV
                     DRPDLHYPTHVPTTAAQLMPREPHTKPDLFGAIARRDVLLHHPYESFATSVQAFLEQA
                     AEDPDVLAIKQTLYRTSGDSPIVQALIDAAESGKQVLALVEIKARFDEENNISWARKL
                     EKAGVHVVYGLVGLKTHCKLALVVRNEGGVLRYYSHIGTGNYNPKTSRLYEDLGLLTA
                     DDQIGKDLTRLFNELSGYAIEKKFKRLLVAPMHLRRGLLKRIERETINARAGRKAGIG
                     IKVNSIVDEALIDALYEASNAGVPVDLWVRGICGLRPGVPGMSENIRVRSILGRYLEH
                     SRIFSFANSGDPQVFIGSADLMHRNLDRRVETLVRLTDPGQVTELNELIAHAFDDTIA
                     SWVLDAEGDWARHHLDAEGRPLGDLHDELMCQITQRPRKGRRP"
     gene            complement(7188..>8119)
                     /gene="mshD"
                     /locus_tag="VFQ96_05720"
     CDS             complement(7188..>8119)
                     /gene="mshD"
                     /locus_tag="VFQ96_05720"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEU0257321.1"
                     /translation="TACVNGRCRAGASPVAWRADGTRPVNEQTELDIAAGRRLLRVAS
                     NEAGESLAAIVTGGDELDLVVTPASRGRGVASEILGDLLPDEPETVSAWSHGDHPAAA
                     RLAATHDFVAARRLLRLVLRDARAGRIDEPGRQVDARRASKAALRPEHTSRAADRIRV
                     DAFRATDADEEESWLRLNARIFADHPEQGRVTLADLHDRMAEAWFTPEDFLVARDEHG
                     AIVGYNWTKVESGAGQAEGEIYVLGVAPERHGQGLGRRLLDAGLDRLARRGCTTITLY
                     VEGDNEPALGLYRAAGFTDDLADTQYRRRSAVA"
BASE COUNT         1152 a         2884 c         2809 g         1274 t
ORIGIN      
        1 agaccgccac gacgcttcgc agcgtggccg gtgccgcgct gcgcgggatg ctcgccgagg
       61 ccctgcccga gctcgaccgg acccgcgagt tgcgcgcccg ttcccgcgga cgaggtcgcg
      121 atctcatggc cgaggtgctt cgccgtgccg tcgagcgcgg cgagatcccg ccagaggtgg
      181 tgctccccgg tcgccttgga gtgggcggcg cactgctgcg cgaccactac ctgttccaca
      241 acgaacccct cgacgacacg gtgctcaccc gcgtggtcga cgacgtcttg ctcccgcttt
      301 tcgtcgcgtc gccgccggcg cgagcatccc gcagcgcctt agagacgagc gcgatggcgc
      361 agaacggcgc gcgcatcctc cgcaaccaga ccgtgcgtga gctcgcgaac gatctgcgcg
      421 cgcaggtggc gcgcgcgccc gggcagaggt ggatgctcgg tgtcaccggc gcgcccggcg
      481 cgggcaagtc cacgctggcc ggctcactcc tggccgaatt gggtccggac ctcgcggcgg
      541 tcgttccgat ggacgggttc cacctcgccc gagcggtcat cgagggcacc gcgctcgccc
      601 ggcgtcgagg cgctcccgac acgttcgatg cggacggata tgtcgccctt ctcgcgcggc
      661 tgcgcaggcg cgacgcggcc gtcgtctacg cgccggcgtt ccggcgtggg ctcgaggatc
      721 cgatggcggc ggcgatcgcg attccccggc acgtgcccgt cgtgatcacc gagggcaact
      781 accttctctg ccacgaccga tcgtgggccg ccgttcgcac gctcctcgac gaggtgtggt
      841 acatcgaggc cagagggcag acccgcaccg cacgcctggt ggctcgacac accgcgttcg
      901 ggatgacacc agagcaggcg agcgcgtggg ctgcggagac ggacgccgcg aacgcccgcc
      961 tcgtcgagtc gacacgcggt gccgccgacc gagtcgtcct ctgggagtga gcccgggcgg
     1021 ccgcagcact tttgacggcg cgaccatcgc tcgcgtcgcc gaggggcttc tcgttgacct
     1081 gcgcgagtac cagacgaccg ggccgcggcc accacactca ggtgaggaac gggatcgcta
     1141 cggggccggg gccgaacacg gcgatgacgg cggccagccc cagcagcatc aggtcgtatt
     1201 cggggccgcc cttctgcgcc cagtaggtgc tgtgccagcg gaagacgtgc atcccgatgg
     1261 tcacgagcat caagaggatc acgccgaggg ccgcccaggt ggcgaggatg ccggtggcga
     1321 gaccgagggc cgccgcggcc tcgacgatgg gcacgaacca cgccaccggc agcggcacgc
     1381 cgtcattctt ggcgaagccc cgcacgtctg tgaacttcaa caccgcctcg cgcacgaacg
     1441 cgacgacgag cagcacccgc acgagcgtca acacgatcac cgcggccacg ccgggaacga
     1501 aagccatctt cggtcacccc ctgcgcgaaa ccctagcggg gaggcggccc gctcgtcact
     1561 acgctggtcg tggcgggagg cgccaggcct gcccccggaa aggcggcgac cgtgaagctt
     1621 ctttcgaaac tctgcgtggt ggcggcttcg gtcgtcgtcg cggccgggct cgccgcggct
     1681 cccgcgacgg cgaagacggc gccgaggcag gcgttctccg acgtgcccgc ctcgtcgcag
     1741 ttcgcggccg cgatcgactg gctcgcgcag aagcacatca cgaccgggta cgcggacgac
     1801 accttccggc ccacccgcac aatcacgcgc gaggcgatgg cggcgttcct ctaccggctc
     1861 gacggctcgc cggcggaggc ggccccggct tccgccccgt tccccgacgt gccacgagac
     1921 ggccatttcg ccaaggagat cgcctggctc gccggcacgc acatcacgac cgggttcgcc
     1981 gacgggggct tccacccgac cgccgccatc acgcgtgagg cgatggcggc gttcctctac
     2041 cggtacgcgg ccatgaaggg caccggcgac acggcctcct accaggagcc cgccacgtcg
     2101 tcgttcctcg acgtcgccca ctccgcccag ttctccttgg aggtgtcgtg gctggcctcg
     2161 accggcatca cgaccggctg gcaggtgggg cgcggctgcg agcagttccg gcccaccgca
     2221 tcgatcacgc gtgaggcgat ggcggcgttc ctctaccgct acgcgacggc gggcgcgaag
     2281 cccggcaccg ccgcccccgt cacctgtgac gcgccgcctg ccggcgaggg ctcgttctcc
     2341 ggcgatgcgg gaaccctcaa ggtcggcacc gccgtgcagc ccggcaccta tgtctcgtcg
     2401 acgccgtctt ccggcccggg atgctcctgg gcccgtctct cggccgccgg cgacgatgcc
     2461 gctgacaccc tggcgagcga tctgggcgcg ccgggatccc aggtcatcgt cacgatcgac
     2521 ccggccgacg cctacttcgc gacctccggc tgcggcacct ggaaggccct caccgggccg
     2581 accgcccaga agcccctgac gacgatcccc ggcgacggcg gcacctacgt cgtcggctgg
     2641 ggcgtgcggc ccggcacata tgcctccacc accccggcct ccggccctgt ctgcttctac
     2701 cgacggctgt cctcggcgga cggctccgtc gcggccatcc tcgacgacga ggtcgcggtt
     2761 cccggcgagc gtgtcgtcgt caccatcgaa ccgggcgacc tgtatttcca caccgagggt
     2821 tgcggcacct ggcaggctct cggctgacgg cacggggctt gccgcgtggg cgacatcggc
     2881 cggatacggt gcttctcgga gccgtcgact gacgagccgc gggcaaagag aggccggccg
     2941 tcgaggcatg acggccggcc ctccccctgt gtcaggagtc tcttgcaatc acattccgcg
     3001 ccggccgtac cagtaaacga ccggcgttcc cgacactcta taacgaattg gtaacaaacg
     3061 gtgacgtggg cggtgtgttt ccgattcgtg gagtgcctgg cgcctcacga cccctcgacc
     3121 agtggcgctt cgcgtcacag ggcctcgctc ctcgttaggg tggagccggg ccccatggcg
     3181 gatcgtgacg gggagggacg acgtgccaga attccagcgg cctcggcggg tgccgagctt
     3241 cgtcttcgac gccctgccgg gggcgcacga cccggccgag tcgaccgcgc tcgcacaccg
     3301 caccgccgcg gcgctcgtct cccgcgtgcg ggccgacccg cggcctccgg tgctcgacag
     3361 gctcgtcgcg ttcgccgacg agcacgggtt ggacgccgtg gcggaactct ggtcggcggc
     3421 ccggccctgc agcctgcccg gcgccctgtg gcgcatctac ctgctgcacg ccgtcgcggt
     3481 gcgctccgcc gaggaatgca gttacctgta tcgacgcggc atggagtcgg cccgcggcat
     3541 cgacccggtc gtcgctggcg ccgcggtccc gaccggcccg cgagaactcg tggcgctcac
     3601 cacactgatc ttgaagggcc tcttcgaggg cgacttcgcg gtcgccctgg agcgcgccgc
     3661 cgcgtattgc cgagtcgtct ccgtcggctg cgcaagcctc gccgacgact ttgacggcac
     3721 cgagccggaa cgctccagcc gcctgacgag gcgagggctg cgctaccaca cgatctccga
     3781 agacctcacc ggttgcgcgg cgctgtggcg ccaaggcgcg ctcgaatgag gctgggcggc
     3841 gtcatcgctc ctcgacgcta ggccgcgacg ccggcacggc ccgcgcttct tcggccgcgc
     3901 ccgatgcttg gcccgggttc ggcgaggcag agaaggatcc gcagggaagg gtccgcgacc
     3961 tcgctcggtt acactggtgc gtggccgggc cgtggtaacc ccgggctcca tcattcgccg
     4021 cttcgagcgg cctttcgccg agaggcgttc tgcggtccgg cccgctcggc ctaccgatgc
     4081 tcggggtgtg tctcgatggc gaggatgccc gcaccggggt tcgagacgga gagatgcacg
     4141 acgctgaagc cgcccgtcgt gagcctcgcc gcatcccgca gctcggaggg tgcctcgccg
     4201 ccggccgcga gcgcgatctc gcgcaggatc acggggagca ccggcccgtg gctgcacaag
     4261 accgcggtgc ggcgcttgcg aacccgtttg ccgacgatct ggcgcaccgt gccctcgccg
     4321 gcctcgtgcg cgtcttggct gaggccttcc tcccgcaccg cgcgcacgcc gaacgacgcg
     4381 gcaagaggcg ccacggtccc cacgcagcgc acggcggtgc tcgtgaggag cttgcgcggc
     4441 cgccatgccc cgatcggccc gacgttcgcc gacgcctgcc gggcgccgtg gtcggtgagt
     4501 gggcgcgtcg catccggccc atcccattcc gtagcgggca cggcctgccc gtgccgcagg
     4561 ccgatcaggg cgaaggtact cgtgacgccc cgctccacga gggcggcgaa cgtgtcgacg
     4621 atggccacgt cgcgctcgta gctaaggtat tttcgcgctt tcttcagccc gacccattcg
     4681 agcgccgcga tctcgtggtt cggcacgaag gtggagcggg ccaacgcctc ctcggccact
     4741 tctgccgccc aatactgcac gttcttggta cgcccgccgc cgatcgtgta gctcgtcgtg
     4801 ccgagcggaa ccccgaggac gacggcgagc cccgtctctt cgcggatctc ccgcaccgcc
     4861 gtctgcggca gggcctcgcc cggctccacc ttgcccttcg gcaggctgac gtcgccgtac
     4921 ttggcgcggt gcacgacgag gatggcgggg gtcttgccct ctcggggccg ccagcagacc
     4981 gccccggcgg cgaagacctg cttcatgggc ggcggccctt cctggggcgc tgcgttatct
     5041 gacacatcag ctcgtcgtgc aggtcgccca acggccggcc ttccgcgtcg aggtgatggc
     5101 gcgcccagtc gccctccgcg tcgagaaccc acgaagcgat cgtgtcgtcg aaggcgtggg
     5161 cgatcagctc gttcagctcg gtgacctggc ccgggtcggt gagccgcacc aaggtctcga
     5221 cccggcggtc gagattgcgg tgcatgaggt cggcgctgcc gatgaacacc tgcgggtcgc
     5281 cgctgttggc gaacgagaag atgcgcgaat gctccagata tcggcccagg atggaacgca
     5341 cccggatgtt ctcgctcatg cccggcacgc ccgggcgcag cccgcagatg ccccgcaccc
     5401 acagatcgac gggcacgccg gcgttgctgg cctcgtagag agcgtcgatg agcgcctcgt
     5461 cgacgatcga gttgaccttg atgccgatgc cggccttcct gccggcgcgg gcgttgatcg
     5521 tctcgcgttc gatgcgcttc aagagtccgc gtcgcaggtg catcggcgcg acgagcagtc
     5581 gtttgaactt cttctcgatc gcatagccgc tcagctcgtt gaagagcctg gtcaggtctt
     5641 tgccgatctg gtcgtcggcg gtcaaaaggc cgagatcctc gtagagccgg ctcgtcttcg
     5701 ggttgtagtt gcccgtgccg atgtgactgt aatagcgcag cacgccgccc tcgttacgca
     5761 cgacgagcgc cagcttgcag tgcgtcttca agccgaccag tccgtagacg acgtgcacgc
     5821 ccgccttctc cagcttgcgg gcccaggaga tgttgttctc ctcgtcgaag cgcgccttga
     5881 tctccaccag cgccaagacc tgcttgcccg attctgcggc gtcgatgagg gcctgcacga
     5941 tcgggctgtc gcccgaggtg cgatagagcg tctgtttgat ggccaagacg tcggggtctt
     6001 cggcggcctg ctccaagaac gcctgcacac tggtcgcgaa cgactcgtac gggtggtgca
     6061 gcagcacgtc gcgccgggcg atcgcaccga acaggtcggg cttcgtgtgc ggctcccgcg
     6121 gcatcagctg ggctgcggtc gtcggcacgt gcgtcgggta gtgcaggtcg ggccggtcca
     6181 cgtgggcgag atcgaagagg ccgcccaggt cgagcaggcc ggggatgcgg tagacctctt
     6241 gttccgacac gtcgagctcg cgcacgagaa ggccgagcgt cacctcgtcc atgtcggcgg
     6301 tcacctcgag gcgcacgggc ggcccgaagc ggcggcgcag gagctccttc tcgagagcct
     6361 gcagcaggtt ctccgtctcg tcttcgtcga cctcgacgtc ctcgttgcgg gtcacgcgga
     6421 acacgtggtg atcgagcacc tccatgcccg cgaagaggtc gtccagatgc actgcgatga
     6481 ggtcttccag cgcgatgaag cgcatccgcg tgaggggctc gcggttgtcg acccgcaaga
     6541 ggcgcggcat catctgcggc accttgaggc gcgcgaactg ttccttgccc gtcttcgggt
     6601 tgcgcacccg caccgagagg ttcaacgaga gcccggagat gtaggggaat ggatgcgccg
     6661 ggtcgacggc cagcggcatc aacaccggaa agatgcgggt gttgaagagc acgtgcatct
     6721 gctgccggtc cgcctcgtcg aggtcggccc acgacacgag ggttatgccc gcctcggcga
     6781 gctccggcag cactgcgtcg tgcagggccc gggcatgccg gtcctgcagc tcgtgggcct
     6841 tccgggagat gtcggccagc acgtcgagag gcgcctgacc ggtgttggtc ggcacggcca
     6901 agccggtgag gatgcgtcgt ttcaggccgg cgacccgcac catgaagaac tcgtcgaggt
     6961 tgctggcgaa gatcgccaga aagttcaccc gctcgaggac cgggatcgac gggtcttcgg
     7021 cgagctcgag cacgcgcaga ttgaaggcca gccagctgag ctcgcggttg aggtatcgat
     7081 cgtcgggcag ggtgccggtc ttctcgatgg ggccggaggt gaagtcgtcg ttgaggtcgc
     7141 tgtttgcctc cgcgccgacc agcagatttt cggcgtccat cacctcatca tgccaccgcg
     7201 cttcggcgcc ggtattgggt gtcggcgagg tcgtctgtga accccgcggc acggtacagg
     7261 cccaaggcgg gctcgttgtc gccctccacg tagagcgtga tcgtcgtgca gccgcgtcgg
     7321 gcgaggcggt cgaggcccgc gtcgaggagc cggcggccga gcccttgccc gtgccgctcg
     7381 ggggcgacgc cgagaacgta gatctcgccc tcggcctggc cggcacccga ctccaccttc
     7441 gtccagttgt agccgacgat cgccccgtgc tcgtcgcggg ccacgagaaa gtcctcgggc
     7501 gtgaaccagg cctccgccat gcggtcgtgc aggtcggcca gagtgacgcg gccttgttca
     7561 gggtggtcgg cgaaaatacg cgcattgagc cgcaaccaag actcttcctc gtcggcgtct
     7621 gtcgcccgga acgcgtcgac gcggatgcgg tcggcggccc gggaagtgtg ctcgggccgc
     7681 aaggcggcct tcgacgccct tctggcgtct acctgccggc ccggctcgtc gatccggccc
     7741 gcgcgggcgt cgcgcaacac gagacggagc aggcgccggg ctgcgacgaa atcgtgggtg
     7801 gcggcgagcc ttgccgcggc cggatggtcg ccgtgcgacc aagcgctgac cgtctccggc
     7861 tcatcgggca agaggtcgcc caggatctcg gaggcgacgc cacgaccgcg gctcgcgggt
     7921 gtgacgacga ggtcgagttc gtcgcctccc gtgacgatcg cggccaggct ctccccggcc
     7981 tcgttcgacg cgacacggag cagccggcgg cctgccgcga tgtcgagctc cgtctgctcg
     8041 ttgacgggcc gcgtgccgtc ggcccgccag gcgaccggtg acgcacccgc ccgacatctt
     8101 ccgttaacgc acgcagtgg
//
LOCUS       DASTNL010000185         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_693011, whole genome shotgun sequence.
ACCESSION   DASTNL010000185 DASTNL010000000
VERSION     DASTNL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693011"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VFQ96_05725"
     CDS             <1..1311
                     /locus_tag="VFQ96_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0257322.1"
                     /translation="AADGVTVATPSGAVGLEAEIVVVAGLQDGAWPDLRPRGSLLHAD
                     ALVEVLSGLAGTPIDERRRVLDDELRMFALALSRARRQVVVTALESEDESPSPFLDLL
                     PPLPRIEAPEGASPLDLRGMVGVLRRQLTAPEAGPEERDAAASGLAGLAAAAVPGADP
                     AEWLGLGGPSTTEPLFTPDEDVPLSPSHLESLETSTLDWFIDDISADPPGLAAGVGTL
                     LHWAMQEAARPDLDAVWQVVLERWGELSFEAPWLERRERAAARRLAAGLAEYLADARR
                     AGRVRLAAEQRFTLRVGRATMNGSVDRLEKTADGRVVIVDLKTGRPVTSAEAIAAHPQ
                     LSAYQAAYRDGRFDEVLTPFGPHRLGGASLLFVRDGIGGKLYRDEAQDAIDEVGLRAF
                     RSRVENAARLLTATALPGPRTIDDRHAASAVERLFRRIAEVTHG"
     gene            1304..>3753
                     /locus_tag="VFQ96_05730"
     CDS             1304..>3753
                     /locus_tag="VFQ96_05730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEU0257323.1"
                     /translation="MGERKAMFSAARLAATLGLPEPTPQQKAIIESPLGSALVVAGAG
                     SGKTETMSSRIVWLLANGWAKPQDILGLTFTRKAAGEMAARFRARVTALGETGLLPGD
                     RDPFDTVTVATYNSFANSIYRDHALLLGREPDGVVLGEASAWRLARRVVLASRDERLA
                     ALDKSAETLADAVLALSRALASNVADGETVAAMAREFTAVAGLPPGGRGLYAQVEKWA
                     LDVGALDLLVDLAARYQREKVTRGVIEYSDQVALALGIVESDDAVRREIRGRYRVILL
                     DEYQDTSVVQTRLLSTLFRGRAVLAVGDPNQSIYGWRGASAAGLARFPAQFDAETTFR
                     LTVSWRNGRRILDAANTLAAPLAGSLPVARLQAAPHAGASPVDVRYEQTVESEADVTA
                     GWLAERLSEGGATKPTAALLLRSRAQQQVFLDALERRAVPYHVLGLGGLLEQPAVADL
                     VSALRVVADVSSGGALVRLLAGARWRIGLRDLRALRRVAGWLAAHDAGGRPLAPEVRR
                     RLRGSVAEGEGESLSDAVDFVRTAPPSHPALAVLSAEGRRRIGEAGALFARLRARRGL
                     GLHELAGLARQELLIDIELLANEARPATHAGLDAFSDAIDDYLAVGDEPDLRGFLHWL
                     AEAARRDGLGPRPEEPEAGTVQVLTIHGAKGLEWDHVAVPLWTAGDLPARQHDGTSGW
                     LRFGEMPYEFRGDRRDLPSLPWRSAATRKELVTAVDGFKKEVTARHADEERRLAYVAL
                     TRARHRLLLSGSFWATGKRPRLPSPFLADLAEAGLIAQPPAAPDDEANPRHPETDVVV
                     WPADPLGTRRVRLEEAARR"
BASE COUNT          490 a         1354 c         1369 g          540 t
ORIGIN      
        1 gccgccgacg gcgtcaccgt cgccacgccc tctggcgcgg tcgggctcga ggcggaaatc
       61 gtcgtcgtcg cgggcttgca agacggcgca tggcccgacc tgcgtccgcg cggctcgttg
      121 ctgcacgccg acgcgctggt cgaggtgctc tctggcctgg cgggcacgcc gatcgacgag
      181 cgccgccgcg ttctcgacga cgagctgcgc atgttcgcgc tggccctctc gagagcccgg
      241 cggcaggtcg tcgtgacagc gctcgagagc gaagacgagt cgccgagccc gttcctcgat
      301 ctcctgccac cgctgccgcg catcgaggcg cccgagggcg cgagcccgct agacctacga
      361 ggcatggtcg gtgtgctgcg ccgccagctg acggcgcccg aggccggccc cgaggaacgc
      421 gatgcggccg cgtcggggct ggcggggctg gcggccgctg cggtgccggg cgccgatccc
      481 gccgaatggc tcgggctggg cgggccttcc accacggaac cgctcttcac ccccgacgag
      541 gacgtccctc tttccccctc tcacctcgag tcgctcgaga cctcgacgct ggactggttc
      601 atcgacgaca tctcagccga cccgccgggc cttgcagcag gcgtcgggac cctcctgcat
      661 tgggcgatgc aagaggcagc ccggcccgac ctcgatgcag tctggcaggt ggttctcgag
      721 cgctggggcg agctgtcgtt cgaagcgccg tggctggagc gccgggaacg agccgccgcc
      781 cggcggctgg ccgccgggct cgcggaatat ctcgccgacg ctcggcgtgc ggggcgggtg
      841 cgcctcgccg ccgagcagcg tttcacgctc cgggtcggcc gcgccacgat gaacgggtcc
      901 gtcgaccgtt tggagaagac ggccgacggc cgcgtcgtca tcgtcgacct gaagaccggg
      961 cggccggtga cgagtgccga ggcgatcgcc gctcatcccc agctgagcgc ctaccaggcc
     1021 gcctaccgcg acggccgatt cgacgaggtg ctcaccccgt tcggcccgca ccggctgggt
     1081 ggcgcgagcc tcctgtttgt gcgggacggc atcgggggaa agctctatcg cgacgaggcg
     1141 caggatgcga tagacgaggt ggggctgcgg gcgttccgct cccgcgtcga gaatgcggcg
     1201 cggctactga cggcgacggc acttcccggg ccccgcacca ttgacgatcg ccacgccgcg
     1261 tcggcggtcg aacgcctgtt ccggcggatc gccgaggtga cccatgggtg agcggaaggc
     1321 gatgttttcg gccgcccggc tcgcagcgac gttgggcctg cccgagccca cgccgcagca
     1381 gaaggcgatc atcgagagcc ctctcggctc ggcgctcgtc gtcgcggggg cgggtagcgg
     1441 caagaccgag accatgtcgt cccgaatcgt gtggctgctc gcgaacggat gggcgaagcc
     1501 gcaggacatc ctcgggctca cgttcacccg caaggcggca ggcgaaatgg cggcacgatt
     1561 ccgcgcccgt gtcacggccc tcggcgagac ggggctgttg cccggcgacc gtgacccgtt
     1621 cgacacggtg acggtggcga cctacaactc cttcgcgaat tcgatctacc gcgaccacgc
     1681 gctccttctc ggccgcgaac ctgacggtgt cgtcctgggg gaggcgtcgg cctggcgtct
     1741 cgcccggcgc gtcgtgctcg ccagccgaga cgaacggctg gccgcactcg acaagagcgc
     1801 cgagacgctg gccgacgcag tcttggcgct cagccgagcc ctcgcctcca acgtcgccga
     1861 cggcgagacg gtcgccgcga tggcccggga gttcacggcg gtcgcgggcc tcccccccgg
     1921 cggcaggggg ctgtatgcgc aggtcgagaa gtgggcgctc gacgtgggtg cgctcgacct
     1981 gctggtcgac ctcgcggcgc gttaccagcg tgagaaggtg acgcgcggcg tcatcgaata
     2041 ctccgaccag gtcgcgttgg cgctcggcat cgtggagtcg gacgacgccg tgcggcggga
     2101 gatccgcggg cggtaccggg tgatcctcct cgacgagtat caagacacct cggtcgtgca
     2161 gacgagactc ttgagcaccc ttttccgcgg ccgggccgtc ttggcggtcg gcgacccgaa
     2221 ccagtccatc tacgggtggc gcggggccag cgcggccggc ctcgcacgct tccccgccca
     2281 gttcgacgcg gagacgacgt tccggctcac cgtcagctgg cgcaacggcc ggcggatcct
     2341 cgacgcggcg aacacccttg ccgcgccgct tgcgggctcc ttgccggtgg ctcggctgca
     2401 ggccgcgccg cacgccggtg cctcgcccgt cgacgtccgc tacgaacaga cggtcgagag
     2461 cgaagccgac gtcaccgccg gctggctcgc cgagcgttta tcagaaggag gggcgacgaa
     2521 gccgaccgcc gcactgttgc tgcgctcccg agcccagcaa caggtcttcc tcgacgcgct
     2581 cgagcgtcgc gcggtgccgt accacgtgct gggtctgggg ggcctcctcg agcagccggc
     2641 cgtcgccgac ctggtcagcg cgctgcgggt ggtcgccgac gtctcttcgg gcggggcgct
     2701 cgtgcgcctc ctcgccgggg cgcgctggcg catcggcttg cgcgacctgc gggcgctgcg
     2761 ccgcgtcgcc gggtggctgg ccgctcacga cgccggcggg cgccccttgg cacccgaggt
     2821 gcgccgcagg ctgcgcggct ccgtcgccga gggggagggc gagtccctca gcgatgccgt
     2881 cgacttcgtc agaacggccc cgccttctca cccggcgctc gcggtgctca gcgccgaggg
     2941 tcgccgcagg atcggcgagg ccggcgccct cttcgcccgg ctgcgcgcga ggcgcggcct
     3001 cggcctgcac gagctggccg gcctcgcccg gcaagagctg ttgatcgaca tcgagctcct
     3061 ggcgaacgag gccaggccgg cgacgcacgc cgggctcgac gcattctctg acgccatcga
     3121 cgactatctg gcggtgggcg acgagcctga ccttcggggg ttcctgcatt ggctcgccga
     3181 ggcggcccgg cgcgacggcc tcgggccgcg gcccgaagag ccggaggcag ggacggttca
     3241 ggtgctcacg atccacggcg ccaagggcct ggaatgggac catgtcgccg tgcctctgtg
     3301 gaccgcgggg gatctgccgg cgaggcagca tgacggcacc tcgggttggt tgcgcttcgg
     3361 cgagatgccg tacgagtttc gtggcgaccg gcgcgacctg ccgagtctgc cgtggcgttc
     3421 ggccgccacc cgcaaagagt tggtgacggc ggtcgacggc ttcaagaaag aggtcacggc
     3481 ccggcatgcc gacgaggagc gccgcctggc ctacgttgcg ctcacccgcg cccgacaccg
     3541 gcttctgctg agcggctcct tctgggcgac gggcaagcga ccccggctcc cgagcccctt
     3601 cctcgccgac ctcgccgagg caggcctcat cgcgcagccg ccggcggcgc ccgacgacga
     3661 ggccaatccc cgtcatcccg agacggacgt cgtcgtttgg ccggccgacc cgctcggcac
     3721 gaggcgggtg cgcctcgaag aggccgcacg tcg
//
LOCUS       DASTNL010000186         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_693489, whole genome shotgun sequence.
ACCESSION   DASTNL010000186 DASTNL010000000
VERSION     DASTNL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693489"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFQ96_05735"
     CDS             complement(<1..642)
                     /locus_tag="VFQ96_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0257324.1"
                     /translation="MDPKDRDRLVADYLPLVGYLVSELCARATQLDRDELASAGQFAL
                     FQASRGFDPERGVPFGSYARTRISGALADELRSRDWVSRGTRKRIKETLAVTEGLTAS
                     LGRAPGLDEIAAALGCDRESVAALLHDARSGPVLLSERADELTAAIVGPEESAEVRER
                     DAFLRMAVETLPERQRLIVTRIYFEDKQVKEVAAELGVTHAAVSLARSEAMRML"
     gene            complement(854..2338)
                     /gene="purF"
                     /locus_tag="VFQ96_05740"
     CDS             complement(854..2338)
                     /gene="purF"
                     /locus_tag="VFQ96_05740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU0257325.1"
                     /translation="MVHGDGLLSHDLLPGEKGPQDECGVFGVWAPGEEVAKLTYFGLY
                     ALQHRGQESAGIATSDGEKIVVYKDMGLVAQVFNEAALNTLTGHIAVGHTRYSTTGSS
                     RWQNAQPTLGRTATGSVALGHNGNLTNTAHLLRLVHHRYPDLKGELTHGNTTDTAVIT
                     ALFTGDTEHTLEETAREVLPKMLGAFSLVFMDETTLYAARDPQGVRPLVLGRLERGWV
                     VASETAALDIVGASFVREVEPGELIAVDENGLRTSRFEKTRRAGCVFEYVYLARPDTT
                     INGKNVYEARVEMGRTLAAEHPAEADLVIPTPESGTPAAIGYAQASGIPFGQGLVKNA
                     YVGRTFIQPSQTIRQRGIRLKLNPLREVIRGKRLIVVDDSIVRGNTQRALVSMLREAG
                     AAAVHVRISSPPITWPCYYGIDFASRAELIASGLSTEEVRQSIGADSLGYLSVAGMIR
                     ATGQPEDSLCTACFTGRYPIDIPDARLGRGKSLLEGAVIGRRDG"
     gene            complement(2350..2994)
                     /locus_tag="VFQ96_05745"
     CDS             complement(2350..2994)
                     /locus_tag="VFQ96_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257326.1"
                     /translation="MHDQPAQEDSVSEEINPEGAKASGVNPAHDGLSRPVARPLGEAG
                     AAEAGAAEAGAEGRPGPSAEIRRAQVSVRRSPRYGRFIAIVIGIFIVASFFGTFLLPV
                     GGDYTQAQVFGYLVLLSAALGLPVGSLIALAFDRALKRTARTLAAERTDIRGIASNGT
                     PAAEGRGIASNGSAVAEGRGGAPAAGGPPSRGGAPAPRRETPGDGRTTASETSE"
     gene            complement(3044..3355)
                     /locus_tag="VFQ96_05750"
     CDS             complement(3044..3355)
                     /locus_tag="VFQ96_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HEU0257327.1"
                     /translation="MAVELAAGRPVVATATRYLLQMLADAAPGHTVEVRVPPFGAVQC
                     VEGPRHTRGTPPNVVEMVPAVWVAVARGFIGWREAVEAGVIHASGQRADLGSLLPLPG
                     L"
BASE COUNT          547 a         1219 c         1167 g          536 t
ORIGIN      
        1 taacatgcgc atcgcctcgg aacgggcgag cgacacggcg gcgtgcgtca cgcccagctc
       61 ggcggcgacc tctttgacct gcttgtcttc gaagtagatg cgcgtcacga tcagccgctg
      121 ccgctccggc agtgtctcga cggccatgcg caagaacgcg tcacgctccc gcacctcggc
      181 cgactcctcc gggccgacga tcgccgccgt cagctcgtcg gcgcgttcgc tgagcaaaac
      241 cggaccggaa cgcgcgtcgt gcagaagggc ggcgacgctc tcccggtcgc agcccaacgc
      301 cgccgcgatc tcgtcgaggc cgggcgcccg gccgaggctg gcggtgaggc cttcggtgac
      361 ggcgagcgtc tctttgatgc gcttgcgggt gcccctggag acccagtcgc gggagcgcag
      421 ctcgtcggcc aacgcgccgc tgatccgcgt gcgggcgtaa ctgccgaagg gaacacccct
      481 ctcagggtcg aaccctcggg aggcttggaa aagcgcgaac tgcccggccg aggccaactc
      541 gtcacggtcc agctgggtcg cccgggcgca gagctccgag acgaggtagc cgaccaaagg
      601 caaataatct gcgacgagtc gatcacggtc tttgggatcc actgctcttc acctcgatca
      661 cacgcacggc caacgctgcc atgctggccc ccgccacacc cggcatatca gcggcatccg
      721 gcgaaaaaac cggataaccc tcaagcaatg cttaagaact ttcgcataaa tgtcaagtcg
      781 attaagccta gcgacggaga tccttgacat tttcttgagg tggcggggcc ccgacaggcc
      841 cccagggggc ctgttacccg tcgcgtcggc cgatcacggc cccctcgagc agggactttc
      901 cgcgcccgag gcgggcgtcg ggaatgtcga tggggtatcg gcccgtgaaa cacgccgtgc
      961 acaggctgtc ttccggctgg cccgtggcgc gaatcatccc cgcgaccgag agataaccca
     1021 ggctgtccgc gccgatggac tgccgcacct cctccgtgct gaggccagac gcgatcagct
     1081 ccgcacgcga ggcaaagtcg atgccgtagt agcacggcca cgtgatcggc gggctcgaga
     1141 tgcgcacgtg caccgcggcg gcgcccgcct ctcgcagcat cgagacgagc gcgcgttgcg
     1201 tgttcccgcg cacgatcgag tcgtcgacga cgatcagccg tttgcccctg atgacctccc
     1261 gcagcggatt gagcttcagc cggattccgc gctgccggat ggtctgcgaa ggctgaatga
     1321 acgtgcggcc gacgtaggcg ttcttcacga ggccctgccc gaacgggatg ccgctggcct
     1381 gggcgtagcc gatcgcggca ggcgtgcccg actctggggt cgggatcacg agatccgcct
     1441 cggccgggtg ctccgccgcc aaggtacgcc ccatctcgac ccgggcctcg tagacgttct
     1501 ttccgttgat ggtcgtgtcg ggcctggcca ggtagacgta ctcgaagacg cagccggctc
     1561 ggcgcgtctt ctcgaagcgt gaggtgcgca gcccgttctc gtcgaccgcg ataagctcac
     1621 caggctccac ctcgcgcacg aacgaggccc cgacgatgtc gagggcggcc gtctcgctgg
     1681 cgacgaccca gccccgctcg aggcggccga gcacgagcgg gcgcacgccc tgcgggtcac
     1741 gggccgcata cagggtcgtc tcgtccatga agacgagaga gaaggcgccc agcatcttcg
     1801 gcaagacctc gcgggcggtc tcttccagag tgtgttcggt gtctccggtg aacaaggccg
     1861 tgatgacggc cgtgtcggtc gtgttgccgt gcgtcagctc gcctttgagg tcagggtagc
     1921 gatgatgcac gagccgcaac aggtgggccg tgttcgtgag gttgccgtta tgtccgaggg
     1981 cgaccgaccc ggtggcggtg cgcccaaggg tgggctgcgc gttctgccac cgagaggagc
     2041 ccgtcgtcga atagcgggtg tggccgaccg cgatatgccc ggtgagcgtg ttcaaggccg
     2101 cctcgttgaa gacctgcgcg acgaggccca tgtctttgta gacgacgatc ttctcgccgt
     2161 cgctggtggc gatgccggcg gattcctgcc cgcggtgctg caacgcgtag aggccgaaat
     2221 aagtgagctt ggctacctcc tcgccgggcg cccacacccc gaagacgccg cattcgtctt
     2281 gcggcccctt ctcaccgggt agtagatcgt ggctgagaag accgtcacca tgcaccacgg
     2341 atgctccctc tattcactcg tctcactcgc cgtcgttcgc ccgtcacccg gagtctctcg
     2401 gcgcggcgcc ggggcaccgc cacggctcgg cgggcccccg gcggccggcg ccccgccacg
     2461 gccctctgcc acggcgctcc cgttcgaggc gatgccgcgg ccctctgccg cgggggtccc
     2521 gttcgaggcg atgccgcgaa tgtctgtgcg ctcggcggcg agcgtgcggg cggtgcgctt
     2581 gagcgcccgg tcgaaggcca gcgctatcag gctgcccacc ggcaggccga gcgcggccga
     2641 gaggagcacg agatagccga agacctgcgc ctgggtgtaa tcgcccccga cgggaaggag
     2701 gaaggtgccg aagaacgagg cgacgatgaa gatgccgatg acgatcgcga tgaaacgccc
     2761 atagcggggg gaacgtcgca cgctcacctg ggcccgccga atctcggccg acggcccggg
     2821 cctgccttcg gcgccggcct cagcggcgcc ggcctcagcg gcgccggcct ctcccaacgg
     2881 cctcgccacc ggtcgtgaga ggccgtcgtg ggccgggttc acgcctgagg ccttcgcacc
     2941 ctctgggttg atctcttctg acacgctgtc ttcctgcgcg ggctggtcgt gcacgggtgt
     3001 ccattctccc gcatacggct gggaacgctc gaaccaagcc gtctcataaa ccaggcaaag
     3061 gcagaaggga tcccaggtcg gcgcgttgac cggaggcatg gatcacaccg gcctcgaccg
     3121 cctctcgcca gccgatgaag ccgcgggcca ccgccaccca gacggcgggc accatctcga
     3181 ccacgttggg cggcgtgccg cgggtgtgcc gtgggccttc gacgcattgc accgcaccga
     3241 agggcggcac acgcacctcg acggtgtggc cgggggccgc gtcggccaac atctgcagca
     3301 ggtaccgcgt cgccgtcgca acgaccggcc gcccggcggc aagctcgacc gcgatcgtcg
     3361 gccccgacgc ccggccgccc gtgttctcag ccgaccgccg cacgcgaaga gacgccgcgg
     3421 ccgcccgcac cgcctcggcg cctatcgcct cgtcaacgcg tgccgccgc
//
LOCUS       DASTNL010000187         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_138780, whole genome shotgun sequence.
ACCESSION   DASTNL010000187 DASTNL010000000
VERSION     DASTNL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138780"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFQ96_05755"
     CDS             <1..290
                     /locus_tag="VFQ96_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257328.1"
                     /translation="QLSSFGALILASSMIPFLLNVYLTARHAPKVTVDDPWGFGRSLE
                     WATSCPPPRHNFTSIPRIRSESPAFDLHHPEAGTRTPAPSHGAPAMSGSRS"
     gene            590..1030
                     /locus_tag="VFQ96_05760"
     CDS             590..1030
                     /locus_tag="VFQ96_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU0257329.1"
                     /translation="MTIDVASTKTGGNRPGRGGSRSRRVPTTLTLARDAGALYDASGP
                     VSRLIDTLGNNVVADLLGVSRSQPSRWRSGAERLSPQNRKRVSDLDYVLDRLLLELYP
                     EEAGLWLTGSNPHLGGARPIDVLILQGVGAVLPAIDALATGAFA"
     gene            1038..1586
                     /locus_tag="VFQ96_05765"
     CDS             1038..1586
                     /locus_tag="VFQ96_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU0257330.1"
                     /translation="MTTLYRVLPYAADSAKGAAGHPLYIPPTAGAGRVDGPEYDVLYL
                     GDDPVCAVAEAFGALPVWGPGMLRGLPTLPHSVRALATYELASGSTICDLDDAATLLD
                     LDIRPSRVVTRDREVTQGWASRIHRLGRHVGVRWWSYYNPDWGSLGLWDIRRLRVVGV
                     TALDVTHPALIAAAEAIVRRIV"
     gene            complement(1649..2449)
                     /locus_tag="VFQ96_05770"
     CDS             complement(1649..2449)
                     /locus_tag="VFQ96_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0257331.1"
                     /translation="MSVATLEPGRAGWFTQTGQVMRRWLISTLRQVWGPAMSLIQPAI
                     WIVLFGQVFRSLGRLPEFGSGGYIDYLIPGIMMMTVLYSGAWAGTGYVDDMKSGVLDQ
                     YLTSPVRRSAIVSGQLLQQLLVGIAQSAIVLGIGWLVHARYRGGILGVLVALGVATLL
                     AAVFCCCSAAVAIASRSQVALIGLSQIVVLPATFLSTTMMPATLMLAWVRAVSRWNPM
                     TWAVELGRGALGGAYPAAGWWQVVGLASLAAAAFLWALHSLAAYRRSM"
     gene            complement(2446..>2996)
                     /locus_tag="VFQ96_05775"
     CDS             complement(2446..>2996)
                     /locus_tag="VFQ96_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU0257332.1"
                     /translation="VAAALVHRPRVLFLDEPTTGLDPEARAAMWAQIRRLRAHDALTV
                     VLTTHYMEEADRLADRLLLVDRGRAVTQGTPEELKAALHGDTLKVSLIEPDRGAVHAA
                     VSGLRGLHDLVVEASGAEGSLTARTDDASAGVGAVIAALDAAGIRYGQVAASHPTLDD
                     VYLHFVGHTFGASADLTEGIAA"
BASE COUNT          499 a         1126 c          967 g          404 t
ORIGIN      
        1 accagctctc ctccttcgga gccctgatcc tggcctcctc catgatcccg ttcctcttga
       61 acgtctacct caccgcccgc cacgcaccca aagtcaccgt cgacgacccg tggggcttcg
      121 gacgctccct ggaatgggcc acctcctgcc cgccaccccg acacaacttc acctcaatcc
      181 cccgcatccg cagcgaatcc cccgccttcg acctccacca ccccgaagcc ggcacacgaa
      241 cccccgctcc gtcgcacggc gcgccggcca tgtctggctc tcgctcgtga cgggctactg
      301 accgcagggc ggcacgacgg acgcgaagcg agccctcgtc cacagacaaa cgcccgccgg
      361 cgccacggcc ccacccgggg tcgcagcgcc ggcgggcgtt cggcataccg acgcgaggct
      421 ccggaagacc acgccggcgc cggcccgtca tccccacccc acaccgagag gccgccttcg
      481 gccacacccg gtgcgcgacg gcgaaagccc cggcgaagca cggtcgcccg gtcgacgttg
      541 tcaccaaccc acccaacggt tagtgttgac catggacggg ggagggccga tgaccatcga
      601 cgtggcgagc acgaaaacgg gcggcaaccg gccgggccgc ggcggcagcc gttcccgacg
      661 tgttccgacg accctcaccc tcgcgcggga cgccggggcg ctgtacgacg catccgggcc
      721 ggtctcccgc ctcatcgaca cgctcggcaa caacgtggtg gccgaccttc tcggcgtctc
      781 ccggagccag ccgtccaggt ggcgctccgg cgcggaacgc ctctcgccgc agaaccgcaa
      841 gcgagtctct gacctcgact acgttctcga ccgccttctg ctcgagctct atccagaaga
      901 ggccggtctc tggctgaccg ggtcgaaccc gcacctcggg ggagccaggc ccatcgacgt
      961 gctcatcctg cagggcgtcg gcgcggtctt gcccgccatc gacgccctgg cgacaggcgc
     1021 cttcgcctga ccggccgatg acgaccctgt atcgcgtgct gccctacgcg gcggattccg
     1081 caaagggcgc ggccggccac ccgctctaca taccgcccac tgcgggcgcc ggccgggtgg
     1141 acgggcccga gtacgacgtg ctctacctgg gtgacgaccc ggtctgcgcc gtcgctgagg
     1201 cgttcggtgc cctgcctgtc tggggcccgg gcatgctacg tggcctgccc accctgccgc
     1261 acagtgtcag ggctctggcg acctacgagc tcgcatccgg ctcgacgatc tgcgacctcg
     1321 acgacgcggc caccctgctg gatctcgaca tccgcccctc ccgcgtcgtc acccgcgatc
     1381 gcgaggtcac gcaaggctgg gcatcgcgga tccaccggct cggccgtcac gtcggggtgc
     1441 gatggtggtc gtactacaac cccgactggg gcagcctcgg cctctgggat atacgccgtc
     1501 tgcgcgtcgt cggcgtgact gcgctcgatg tgacgcatcc cgcactcatc gcggccgccg
     1561 aggccatcgt ccgccgaatc gtctgagcgt ccccgaccgg cccacccgga gggcggcagc
     1621 ggcgagaaga acctgtcgcg tcgtgccgct acatcgaacg ccgataggcc gccagcgaat
     1681 gcaaggccca cagaaacgcg gccgcggcaa ggctcgccag gccgacgacc tgccaccaac
     1741 cggccgccgg gtacgcgccc ccgagcgcgc cgcgaccaag ctccacggcc cacgtcatgg
     1801 gattccagcg agagacggcc cgcacccagg ccagcatgag ggtcgccggc atcatggtcg
     1861 tcgacaggaa cgtggcaggc aggaccacga tctgcgatag gccaatcaag gcgacctggc
     1921 tgcgcgaggc gatcgcaacc gccgcggagc agcagcagaa gacggcggcc agaagcgtcg
     1981 ccacgccgag ggccacgaga acgccgagaa tccctccgcg gtaccgggca tggaccagcc
     2041 atccaattcc gagcacgatc gccgactgcg cgatgcccac gagcagctgc tgaaggagct
     2101 gtccagagac gatcgcggaa cggcgcaccg gtgatgtgag gtactggtcg aggacgccgg
     2161 acttcatgtc gtcgacataa ccggtgccgg cccaggcccc ggaatacagc acggtcatca
     2221 tcatgatgcc ggggatgaga tagtcgatgt atccaccgga cccgaattcc ggcaacctgc
     2281 ccaatgatcg gaatacctgc ccgaacaaca cgatccatat cgccggctgg atgaggctca
     2341 tagccgggcc ccatacctga cgcaaggtgg agatcagcca gcggcgcatc acctggccgg
     2401 tctgcgtgaa ccagcccgct cgcccaggct cgagggttgc gacgctcatg cggcgatccc
     2461 ttcggtgagg tcggctgagg cgccgaacgt gtggccgacg aagtgcaggt agacgtcgtc
     2521 gagggtcggg tgggatgcgg cgacctgccc gtaccggatg cccgcggcat ccagcgcggc
     2581 gatcacggcc ccgacgccgg ccgacgcgtc gtcggttcgt gccgtgagag acccctcggc
     2641 acccgacgcc tcgacgacca ggtcgtgcaa cccccggagg ccggagacgg cggcgtgcac
     2701 ggccccacgg tcgggctcga tgagggagac cttgagcgtg tcgccatgca gtgccgcctt
     2761 cagctcttcc ggcgtgccct gggtgacggc acggccgcga tccacgagga gcaggcggtc
     2821 ggcgagccgg tccgcctcct ccatgtagtg cgtcgtgagc accacggtca aggcatcgtg
     2881 cgccctgagc cgacggatct gcgcccacat ggcggcgcgc gcctcggggt ccaggccggt
     2941 ggtcggctcg tcgaggaaca gcacgcgcgg ccgatgcacc aacgcggccg cgacgt
//
LOCUS       DASTNL010000188        13554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1063963, whole genome shotgun sequence.
ACCESSION   DASTNL010000188 DASTNL010000000
VERSION     DASTNL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063963"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFQ96_05780"
     CDS             <1..608
                     /locus_tag="VFQ96_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0257333.1"
                     /translation="NDFTREHGLPGDAEGRAALVARWHERKTAIYTQMVDEGELPARP
                     GVRRLVEAAEAAGWRVAVASTSARPSVVAVLRHAVGEEAARRVRVFAGDIVPHKKPAP
                     DIYLHALAELGAGAEETVVVEDSGVGLAAAVAAGLRTVVTVSAYTTDDDFSRASLVVD
                     SLGEPGTRTRVLQDPHGIAPGDVIDLDVLERVLAVPGPTGV"
     gene            619..1263
                     /gene="dhaL"
                     /locus_tag="VFQ96_05785"
     CDS             619..1263
                     /gene="dhaL"
                     /locus_tag="VFQ96_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU0257334.1"
                     /translation="MPGGFSDVEYVVRTIAQTAVDNETEFSNLDSVVGDGDFGYSLAR
                     GFELVLEKWDDYDRSDIGTFLQKIGVGITGRIGGTSGPIWGTAFLRAAAYCKGRTELA
                     GADVVAVLKAAMEGIKARGGAELGDKTLLDAMAPMTEAIEAQVAQDAEADEVVRAAAE
                     AARAGAEATVPMIARRGRAAYSGERSIGSPDAGATAVAVILERIARDWPVAPGA"
     gene            1345..2340
                     /gene="dhaK"
                     /locus_tag="VFQ96_05790"
     CDS             1345..2340
                     /gene="dhaK"
                     /locus_tag="VFQ96_05790"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU0257335.1"
                     /translation="MKKFVNDPKDYVPEMLKGIALANPDTLTYVPDYNLIMRKDAPRQ
                     DKVSVIQGSGSGHEPAHVLAVGPGMLDGACPGDVFAAPPMDYVYETTKLLASPKGVLL
                     LVNNYTGDRMAFDMAREMSEADGVKVKTLFIDDDVSVKDSTYTVGRRGVAGNFFVMKA
                     VGAATEAGADLDEAVRIGEKVNSVTRTMGLALAPCTPPAKGSPLFELAEDEIEIGVGI
                     HGEPGRRRGTMAPANELLDELLEPVVTDLPYESGDRVALMINGLGSTPISELYILYGR
                     AHEQLAERGITVTRSYVGDYCTSLDMAGASLTLVRLDDEIDRLLEAPASVGMRVF"
     gene            complement(2389..4962)
                     /gene="ptsP"
                     /locus_tag="VFQ96_05795"
     CDS             complement(2389..4962)
                     /gene="ptsP"
                     /locus_tag="VFQ96_05795"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU0257336.1"
                     /translation="MPMIGIVVVSHSPALAQAAVGLALEMVSGTAPRIEVAAGAGDDI
                     IGTDAVRVAEAITTVAGDDGVLVFTDLGSAVLSAQMATELAGVDKVLLTDAPFVEGLT
                     AAVVSAAQGRALHEVEREARGALAAKTAQLAPEGVADTGAAGGPAGRGAETEDATSQG
                     ATSHSVEGSGSPASPERPRPERTPEPTVDALRAEVPLTNPSGLHARPAGMMIGALAGL
                     DARVWVRREGGRGEPVEVTGTTALLMVGARKGDTVEFRAAGADAALALERLTTLAADG
                     FGESDDGPAAAGQVAAGPTTAGPTAAGQPSAGVGVSPGRAVAPIVRRGPGPAEPARRP
                     MPADEREAECVRARSAFAGAADFYRSRAAGQSGQSAQILGVGALLAEDEGLIRAAEQR
                     IREAGDNAEYAVWQAAGQIARQLADAGSLAAERATDLRDVRDRVIALLTHPADTAPGA
                     GGPAATDARPDRYILVAADLAPADTAGLDPARCVGIVTAGGGPTSHTSILARSLGIPA
                     VVAAPQALTYPEGTWLFIDGGTGRIVPDPDEDTRRGAAENATLAFDGTGHTSDGRRIS
                     LLANIGSAADAVLAAGHGAEGVGLFRTEFLFLGRTSEPTFEEQVASYRQALAEFPGRK
                     VVIRTLDAGADKPLPFMAANPEDNPALGVRGFRTASARPEVLQGQLAAIAEAARTARA
                     DVQVMAPMVDTVEEAARFANAARKAGLPLAGIMIETPAAALTAPELFRVVDFVSLGTN
                     DLTQYTMAADRLSGALASLNDPWQPAVLRLIQGVAAAGQAAGKPVGVCGEAASDPNLA
                     VVLVGFGVTSLSMTPRALGPVAARLKAVPFARCVAAARAAAAAHDARSARDAAAAAAE
                     A"
     gene            complement(4966..5565)
                     /gene="dhaL"
                     /locus_tag="VFQ96_05800"
     CDS             complement(4966..5565)
                     /gene="dhaL"
                     /locus_tag="VFQ96_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU0257337.1"
                     /translation="MRRFSAEVTSHKERLTELDSAIGDADHGTNMARGFQAVVGKLDE
                     SRPEAIDAAFKSVGMTLVSTVGGASGPLYGTFFLRFAQALGPTSLTDAAGLGAALRAG
                     LGGVVARGKAVAEDKTMYDAMAPACDAFDAAVAEGAAAGAAASAAADAADRGRDATLP
                     LVARKGRASYLGDRSAGHLDPGATSTALLFHALADALNA"
     gene            complement(5618..6613)
                     /gene="dhaK"
                     /locus_tag="VFQ96_05805"
     CDS             complement(5618..6613)
                     /gene="dhaK"
                     /locus_tag="VFQ96_05805"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU0257338.1"
                     /translation="MKKLINAPDDVLHDSLVGVQAAHPELEVDLERRIVYRGQATRAG
                     KVGVISGGGSGHEPLHGGYVGYGMLDAACAGEIFTSPTPDQMEAATKHVDAGAGVLHI
                     VKNYTGDVMNFEMAAELAGADGIEVESVTVNDDVAVQDSLYTAGRRGVGLTVLLEKIV
                     GAAAESGRDLATVAALARQIIDSGRSMGMALTSCTVPSVGKPTFDLPDDQMEIGIGIH
                     GEPGRSRESVAPASEIARRLVEPVLADLDFTGGPTIVMLNGMGASPLMELYLMYGEVK
                     ALLDAAGVTVARNLVGNYITSLDMAGCSLTVLKADDDILGLWDAPVNTAGMRWGV"
     gene            complement(6751..7698)
                     /locus_tag="VFQ96_05810"
     CDS             complement(6751..7698)
                     /locus_tag="VFQ96_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEU0257339.1"
                     /translation="MSDITALRKVVRDGAPLKRLGGTWGEVLAEFLGTFVLLMFGDGS
                     VAMAVAGLPGSGRTQGPTTFFLGSGDWMLIAWGWAFAVAFGIYIAGGISGAHLNPAVT
                     LAFAVRRKFPWRKVIPYWIAQTVGAFAAAALVYAVYFEAIDAFNKAAKTVRDAAGGIP
                     TYSIFATFPAPYFHGNNWGPFLDQVIGTMLLVMLIAAVVDNYNMGSFSAHLAPLMVGL
                     LVAAIGISFGANAGYAINPARDFGPRLFAWMAGWGHVALPGTVDGQFSWYFWVPIVGP
                     LVGGVVGVLLYDLFIGDVIASRRLAAKLAAEPGPAGTPN"
     gene            complement(7990..8877)
                     /locus_tag="VFQ96_05815"
     CDS             complement(7990..8877)
                     /locus_tag="VFQ96_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEU0257340.1"
                     /translation="MEAIAHEMRVSRSSVSRLLGFARESGLVEVTIRSPQEDTGRLQG
                     AIHQRYGVAVHIVPIPDHTSDIDRLDRVALSAARMLGQFLDSSMTVGIAWGSTLSAVS
                     RHLVPHPLHDVKFVQLNGAGNTRTTGIVYASEILRRFADAYTATSEQFPVPAFFDDAA
                     TKAALWKERGTRRILELQKRADLAIMSLGSPFAVVPSHVYIGGYLDREDYAALRTERV
                     VGDAATVFYRADGSHRDIPINTRASGPDLDALRRVPRRVVIVSGVSKLASLRGALAAG
                     LITDLIIDEGMARALTAAP"
     gene            9155..10885
                     /locus_tag="VFQ96_05820"
     CDS             9155..10885
                     /locus_tag="VFQ96_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU0257341.1"
                     /translation="MARNMDLHRSNVAGLRERPEAQVLVIGGGINGLGTFRDLALQGI
                     DVALVERSDYASGASSASSHMIHGGVRYLENGEFRLVREAVHERNGLLKIAPHYVKPL
                     RTTIPIFRTFSGIVAAPLRFVTHKQRSTVERGALLIKLGLTIYDSFSRDGGTIPRHVF
                     RRSKAALRDLPRLNPAVKYAATYYDASVHEPERLALDVLRDARAAAGPARAANYVAAV
                     GVADGRVRLRDEITGEEFAFHADLIINASGPWTDLTNGALGRTTRLMGGTKGSHIVLD
                     SPELLAACAGRELFFENSDGRIVLIYPLRGKVMVGTTDLEADIAGPIRCTDAEVRYFF
                     DLIAHVFPDVRVDESQIVYRFAGVRPLPAHGDTQPGFVSRDYRIDTDRPANLPGTTVL
                     SLVGGKWTTFRAVAERLTDTALSLLGEPRRVSTNGLPIGGGRDYPADGEARRRWIAEH
                     RDGLSPERAEQLLRRYGTNAEEVIGFLAEHGDDPLAATTEFTVQELEHMVLHEDVVHV
                     HDVLMRRTSSAFDGAVTSAVIEETAAHLQTLLGWSGGRLADEVAETTRILADEHGVRL
                     ERPAAPVGGR"
     gene            10998..12512
                     /gene="glpK"
                     /locus_tag="VFQ96_05825"
     CDS             10998..12512
                     /gene="glpK"
                     /locus_tag="VFQ96_05825"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU0257342.1"
                     /translation="MDQYVIAIDQGTTSSRAIVFDKSGSIISVGQLEHEQIFPKPGWV
                     EHDPQEIWRNTREVIGQALSKANLTRHNIAAIGITNQRETAVVWDKNTGEPVYNAIVW
                     QDTRTQDIVDELAADGGVERFKSIVGLPLATYFSGTKIKWILDNVPGARERAEAGDLY
                     FGNTDSWVLWNLTGGVNGCIHVTDVTNASRTLFMDLKTLQWRDDILHVFGVPKSMLPE
                     IHSSSEVYGVAEPNSLLRETPIAGILGDQQAATFGQAAFEKGEAKNTYGTGNFLIFNT
                     GEEIVESKNGLLTTPAYKLGDAKPHYALEGSIAVTGSLVQWLRDNLGIIGSAPEIQAL
                     AETVEDNGGAYFVPAFSGLFAPYWRSDARGALVGLTRFVNKGHLARAVLEATAYQTRE
                     VLDAVNADSGVPLTELKVDGGMTANNLLMQFQADVIGVPVVRPVVAETTALGAAYAAG
                     LAVGFWESLDDLRGNWQEDRRWEPAMAEAESERLLRNWKKAVTKTFDWVDADTQ"
     gene            12710..>13554
                     /locus_tag="VFQ96_05830"
     CDS             12710..>13554
                     /locus_tag="VFQ96_05830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1758 family FMN-dependent luciferase-like
                     monooxygenase"
                     /protein_id="HEU0257343.1"
                     /translation="MQFGIFTVSDVTRDPVTGTTPSEAERIQDTLTIAQHAEEVGLDV
                     FAIGEHHNPPFWSSSPTTTLAFIAARTRRLIVSTSTTLVTTDDPVKIAEDFSVLQHVA
                     GGRVDLMLGRGNTGPVYPWFGKDIRQGLELAVENYALLHRLWREDVVDFSGNFRTPLQ
                     GFTSVPRPLDGVPPFVWHGSIRTPEIAEQAAYYGDGFFANNIFWPAEHYQRLVELYRQ
                     RFAHYEHGTPEQAIVGLGGQAFMRENSQDAVNEFRPYFDNAPVYGHGPSMEDFTKMTP
                     LTVGSP"
BASE COUNT         2116 a         4590 c         4767 g         2081 t
ORIGIN      
        1 caaacgactt cacgagggag cacggcctgc cgggcgacgc cgaaggccgc gcggcgctcg
       61 tcgcccggtg gcacgagcgc aagaccgcga tctacaccca gatggtcgac gagggtgagc
      121 tgcccgcccg gcccggcgtg cgccgcctcg tcgaggcggc cgaggctgcc ggctggcggg
      181 tcgccgtcgc ctcgacctcc gcaagaccct ccgtcgtcgc ggtgctgcgc cacgcggtgg
      241 gggaggaggc ggcccggcgg gtccgggtct tcgcggggga catcgtgccc cacaagaagc
      301 ccgcccccga catctacctc cacgccctgg ccgagctggg ggcgggggca gaggagaccg
      361 tggtggtgga agacagcgga gtggggctcg ccgccgccgt cgccgcgggt cttcgcaccg
      421 tcgtcaccgt cagcgcctac acgaccgacg acgacttctc gcgggcgtcg ctcgtcgtcg
      481 attccttggg cgaacccgga acccgcaccc gggtgctcca agaccctcac ggtatcgccc
      541 cgggtgacgt gatcgatctc gacgtgctgg agcgggtgct tgccgtgccg ggccctacgg
      601 gcgtgtgaaa ggacaacaat gccgggtgga ttcagtgacg tcgagtacgt ggtgcgcacg
      661 atcgcgcaga cggcggtcga caacgagacc gagttcagca acctcgattc ggtcgtcggc
      721 gacggggact tcgggtattc gctcgcgcgc ggcttcgagc ttgtcctcga gaagtgggac
      781 gactacgacc gcagcgacat cggaaccttc ctccagaaga tcggcgtcgg cataaccggg
      841 cgcatcggtg gaacgtcggg cccgatctgg ggaaccgcgt tcctgcgcgc ggccgcatat
      901 tgcaagggcc gcacggaact ggccggcgcc gacgtcgtcg ccgtcttgaa ggcggcgatg
      961 gagggcatca aggcgcgcgg cggcgcggaa ctgggtgaca agaccctgct cgacgcgatg
     1021 gcgcccatga ccgaggcgat cgaggcgcag gtggcgcaag acgccgaggc cgacgaggtg
     1081 gtccgcgcgg cggccgaggc ggcgcgcgcg ggggccgagg cgacggtgcc catgatcgcg
     1141 aggcggggtc gcgccgccta cagcggtgag cgcagcatcg gctcgccaga cgcgggcgcg
     1201 acggctgtcg cggtcatcct ggaacgaatc gcgcgggact ggccggtggc gccgggcgcc
     1261 tagtcgtcgg gcctgccgcc ggcggcgcct ccccggagta gttgcaacgc atcaccatag
     1321 ggcatcacga aggagttgtg catcatgaag aagttcgtca atgacccgaa ggactacgtt
     1381 cccgagatgt tgaagggcat cgcgctcgcc aacccggaca cgctgaccta tgtgcccgac
     1441 tacaacctga tcatgcgcaa ggatgcgccg cgccaagaca aggtgagcgt gatccaaggc
     1501 tccggctccg gccatgagcc tgcgcacgtg ctcgccgtcg ggcccggcat gctcgacggg
     1561 gcctgccccg gcgacgtttt cgcggcgccg ccgatggact acgtctacga gacgacgaag
     1621 cttctcgcct cgccgaaggg cgtgctgctg ctcgtcaaca attacaccgg cgaccgcatg
     1681 gcgttcgaca tggcgcgcga aatgagcgag gcagacggcg tcaaggtcaa gaccctcttc
     1741 atcgacgacg acgtctcggt caaggactcg acgtacacgg tcgggcggcg cggcgtcgcc
     1801 ggcaacttct tcgtcatgaa ggccgtcggc gcggcgacgg aggcgggggc cgacctcgac
     1861 gaggcggtgc ggatcggcga gaaggtgaat tccgtcaccc gcacgatggg cttggcgttg
     1921 gcgccgtgca cgccgccggc gaaggggtca ccgcttttcg agctggcgga agacgagatc
     1981 gagatcggtg tcggcattca tggcgagccg ggccggcgtc gcggcacgat ggcgccggcg
     2041 aacgagctgc tcgacgagct gttggagccc gtcgtcacgg acttgcccta cgagagcggt
     2101 gaccgggtcg ccctcatgat caacgggctt ggctccacgc cgatctccga gctgtacatc
     2161 ctttacggga gggcgcacga acaattggcc gaacggggca tcacggtgac ccgcagctac
     2221 gtcggcgact actgcacctc tctcgacatg gcaggggcgt cgctgaccct cgttcgcctc
     2281 gacgacgaga tcgaccgcct tctcgaggcg ccggcgagcg tggggatgcg cgtcttctaa
     2341 ggccggggtg ggccgacgac cggcgcggcg gtcgtggtac gtagggcttc aggcctcggc
     2401 ggccgcggcc gccgcgtcgc gggccgaccg ggcatcgtgc gccgcagccg ccgcgcgggc
     2461 ggccgccaca cagcgggcga agggcacggc tttcagccgg gcagcgacgg ggcccagggc
     2521 gcggggcgtc atcgataggc tcgtcacgcc gaaaccgacg agcaccaccg cgaggttcgg
     2581 gtcggatgcg gcctcgccgc acacgccgac gggcttgccg gcggcctgac cggcggcggc
     2641 aacgccctgg atgaggcgta agaccgccgg ctgccacggg tcgttcaggc tcgccaacgc
     2701 gccggagagt cggtcggccg ccatggtgta ctgcgtcagg tcgttcgtgc cgaggctgac
     2761 gaagtcgacc acgcggaaca gctccggggc ggtgagggcg gctgccggtg tctcgatcat
     2821 gatgccggcc agcggcaggc cggccttgcg ggcagcgttc gcgaaccggg ccgcctcctc
     2881 caccgtgtcg accatcgggg ccatgacctg cacgtctgcc cgggccgttc gggcggcctc
     2941 ggcgatcgcg gccagttggc cctggaggac ctcgggcctc gccgacgcgg tgcggaagcc
     3001 gcgcaccccg agggccgggt tgtcctccgg atttgccgcc atgaacggca agggtttgtc
     3061 ggcgccggcg tccagcgtgc gtatcacgac cttgcggccc gggaactcgg cgagcgcctg
     3121 gcggtacgag gcgacctgct cctcgaacgt cggctcactc gtgcggccga gaaagaggaa
     3181 ctcggtgcgg aagagcccga cgccttcggc gccgtgccct gccgccaaga cggcgtctgc
     3241 cgcggagccg atgttggcga ggagcgagat ccggcgtccg tccgaggtgt gtcccgtgcc
     3301 gtcgaaggcg agggtggcgt tctcggcggc gccgcggcgc gtgtcctcat cggggtcggg
     3361 gacgatgcgg ccggtccccc cgtcgatgaa cagccaggtc ccctccggat aggtcagcgc
     3421 ctgtggcgcg gcgacgacgg ctggtatgcc gaggcttcgg gcgaggatgg aggtgtggga
     3481 ggtgggcccg ccgccggccg tcacgatgcc gacgcagcgg gccgggtcga gcccggccgt
     3541 gtcggccggg gccaagtcgg ccgcgacgag gatgtaccgg tccgggcggg cgtctgtggc
     3601 ggccgggccg ccggcgcccg gcgccgtgtc ggccgggtgg gtgagaaggg cgatcacgcg
     3661 atcgcgaaca tcgcgcaggt cggtggctcg ttccgccgcg aggctgccgg catcggccag
     3721 ctgccgggcg atctgccccg cggcctgcca caccgcgtat tcggcgttgt ccccggcctc
     3781 gcggatccgc tgctcggccg cacggatgag gccctcgtct tcggcgagga gcgctcccac
     3841 gccgaggatc tgcgcgctct ggccgctttg gcccgccgcg cggctgcggt agaagtcggc
     3901 tgcgccggcg aaggcgctgc gagcgcggac gcattcggcc tcgcgctcgt ccgccggcat
     3961 cggccgtctc gcgggttcgg cgggtcccgg gccccggcgc acgatcggcg ccactgcccg
     4021 cccggggctg accccgaccc cggcggaggg ctgccccgcc gcggtcggcc ctgcggtggt
     4081 cggccccgct gcgacctgcc ccgcggcggc cgggccgtca tcgctctcgc cgaagccgtc
     4141 cgcggcgagc gtcgtcaggc gctcgagcgc cagcgcagcg tcggcgccgg ccgcgcggaa
     4201 ttcgaccgtg tcgcccttgc gggcccctac catgagcagc gccgtggtcc cggtcacctc
     4261 gaccggttcg ccacggccgc cttcccggcg cacccacact cgggcgtcca ggccggccag
     4321 agcgccgatc atcatgcccg ccgggcgggc atgaaggccg gacgggttcg tcagaggcac
     4381 ctcggcccgc aacgcgtcga cggtaggctc gggcgttctc tctggccggg gccgttccgg
     4441 cgaggccggg gaaccggaac cctcgacact gtggcttgtg gcaccttggc tggtggcgtc
     4501 ttcggtctcg gcaccccggc ccgcagggcc tcccgcagcc cccgtgtcgg cgaccccttc
     4561 gggcgccagc tgtgcggtct tggcggcgag cgcaccgcgc gcctcacgtt ccacctcgtg
     4621 gagcgcccgc ccctgggcgg ccgagacgac ggctgcggtg agtccctcga cgaacggtgc
     4681 gtcggtcaag agcaccttgt cgacgcccgc caattccgtc gccatctggg cgctcaacac
     4741 tgcggagccg aggtcggtga agacgagcac gccgtcgtct ccggcgaccg tcgtgatggc
     4801 ctccgcgacc cgcaccgcgt cagtgccgat gatgtcgtcg ccggcacccg ccgcgacctc
     4861 gatgcggggg gccgtgccgc tcaccatctc taacgccaag ccgacagcgg cctgggccag
     4921 tgcgggggag tgcgagacga cgacgatgcc gatcatgggc atcggtcagg cgttcagcgc
     4981 gtcggccagc gcgtgaaaga gcagcgccgt cgaggtcgcc cccggatcga ggtggccggc
     5041 gctgcggtcg ccgagatagc tggcgcggcc cttgcgcgcc acgagcggca acgttgcatc
     5101 gcggcccctg tcggcggcgt cggcggcggc cgacgcggcg gcgccggccg ccgcgccctc
     5161 ggcgaccgcc gcgtcgaacg cgtcacacgc cggcgccatc gcgtcgtaca tcgtcttgtc
     5221 ttcagccacg gccttgccgc gggcgacgac cccgccgagg cccgcccgaa gggcggcgcc
     5281 gagcccggcg gcgtcggtta gtgaggtggg gccgagcgcc tgggcgaagc gcaagaagaa
     5341 ggtgccgtag agcgggccgc tggcgccgcc caccgtcgag accagcgtca taccgacgct
     5401 cttgaaggcc gcgtcgatcg cctctggacg actctcgtcg agcttgccga cgaccgcctg
     5461 gaagccgcgg gccatgtttg taccgtggtc ggcgtcgccg atcgccgagt cgagctcggt
     5521 caggcgttcc ttgtgcgacg tgacctcggc cgagaaccgg cgcatccagt cgagaagcct
     5581 ctccagaggg atctgcgcat ctgccacgtt cctactccta tactccccag cgcattccgg
     5641 cggtgttgac gggcgcgtcc cacaggccga ggatgtcgtc gtcggccttc agcacggtga
     5701 gcgagcagcc tgccatgtcg agcgacgtga tgtaattgcc gaccaggttg cgggccacgg
     5761 tgacgccggc ggcgtcgagg agcgccttga cctcaccgta catgaggtac agctccatga
     5821 ggggcgaggc gcccatgccg ttcagcatga cgatcgtcgg cccacctgtg aagtcgaggt
     5881 cggccaagac cggctcgacg agtcgacgcg cgatttccga ggcgggggcg acgctctctc
     5941 ggctgcggcc cggctcgccg tggatgccga tgccgatctc catctggtcg tcgggcaggt
     6001 cgaaggtcgg cttgccgacg gaaggcaccg tgcaactcgt cagcgccatg cccatcgacc
     6061 ggccggaatc gatgatctgg cgggcgagtg ccgcgacggt cgcgaggtcg cgacccgatt
     6121 cggcagccgc gccgacgatc ttctcgagca gcaccgtcag gccgacgccg cggcggccgg
     6181 cggtgtatag cgagtcttgc acggcgacgt cgtcgttcac cgtgacagat tccacctcga
     6241 tgccgtcggc gcccgcgagt tccgccgcca tctcgaagtt catgacgtcg ccggtgtagt
     6301 tcttcacgat gtgcagcacg ccggcacccg cgtccacgtg ctttgtcgcg gcctccatct
     6361 ggtcgggcgt gggcgaggtg aagatctcgc cggcgcacgc cgcgtcgagc atgccgtagc
     6421 cgacgtatcc gccgtgcaac ggctcgtgcc cggagccgcc accggagatg acgccgacct
     6481 tgccggcccg ggtggcctgg cctcggtaga cgatccgacg ctccaggtcg acctcgagtt
     6541 ccggatgggc tgcctggacg ccgaccagcg agtcgtgcag cacatcgtcg ggggcattga
     6601 tgagtttctt catgatgcga tcctttccct gccttgcccg tcgggcgggc gcggaaaggc
     6661 gcgcggccgc gacgggtctg cctgtgcccg cggcggtgtg ggcgcctggc atcggcaccc
     6721 gcaccgccgc agcaccacgg ttcggtcggt tcagttcggt gtgcccgcgg ggccgggctc
     6781 tgcggctagc ttcgcggcga ggcggcgcga agcgatgacg tccccgataa agaggtcgta
     6841 gagaagcacg cctacgacgc cgccgaccag cgggccgacg atcggcaccc agaagtacca
     6901 actgaattgg ccgtccacgg tgccgggtaa ggcgacatga ccccagccgg ccatccaggc
     6961 gaagagccgc gggccgaagt cacgggcggg gttgatcgcg tatccggcgt tggcgccgaa
     7021 cgagataccg atcgcggcga cgaggaggcc gaccatgagg ggggcgaggt gggccgagaa
     7081 gctacccatg ttgtagttgt cgacgaccgc cgcgatcagc atgaccaaca gcatcgtgcc
     7141 gatcacctgg tcgaggaagg ggccccagtt gttgccatgg aagtacggcg ccgggaaggt
     7201 cgcgaatatc gaataggtcg gaatgccgcc ggccgcgtcg cgcactgtct tggccgcctt
     7261 gttgaaggcg tcgatcgcct cgaagtagac cgcgtagacg agtgcggcgg ccgcgaaggc
     7321 gccgacggtc tgggcgatcc agtaagggat gaccttgcgc cacgggaact tgcggcgcac
     7381 cgcgaacgcc agggtgaccg cggggttcag gtgagcaccg ctgatgccgc cggcgatgta
     7441 gataccgaag gccacggcga aggcccagcc ccaggcgatg agcatccagt cgcccgagcc
     7501 caggaagaac gtcgtcgggc cttgcgtgcg gccggagccc ggcagtccgg ccaccgccat
     7561 ggcgacggag ccgtcaccga acatcaggag gacgaaggtt ccgaggaact cggcgagtac
     7621 ctcaccccag gtccccccca gccttttcag tggggcgccg tcgcgcacca ccttccgtag
     7681 cgccgtgata tcactcacaa gaagtctcct ttgactgaaa agcaggtcgt cggttggttg
     7741 gatcagctgg gagtggaccc agccgtttca cagttcatcc tcagtcgagc gtgcatccgt
     7801 gtcaatcact ctgcacacac gtgcacgctt ccggcggcag ccgcctcctc ccctcgccgg
     7861 cggccgcgcc gcgctcggcc cggccatcgt cttgtccgga cggtcaacgt gcgggcactg
     7921 cccaaagatt atgctctcgc cgaggcgcct gccagacccg gttcccgcac cgccgtgtgc
     7981 tgcgcgcttt cagggtgctg cggtaagggc ccgcgccatg ccttcgtcga tgatcaggtc
     8041 ggtgatgagg cccgcagcga gggctcccct caggctcgcg agcttcgaga cgcccgagac
     8101 gatgaccacc cgccgcggca cgcggcgcaa ggcgtcgaga tccggaccgc tcgcacgcgt
     8161 attgatcggg atgtcgcggt ggctgccatc ggcgcggtag aagaccgtcg cggcgtcgcc
     8221 gaccacccgc tcggtgcgaa gcgccgcata gtcctcgcgg tcgagatagc cgccgatata
     8281 cacgtggctc ggcacgacgg cgaacggcga gccgaggctc atgatggcga ggtcggcacg
     8341 cttttgcagc tccaggattc ggcgggtgcc gcgctccttc cagagcgcgg ccttcgtcgc
     8401 cgcgtcgtcg aagaacgcgg gcacgggaaa ctgctcgctc gtggccgtgt aggcgtcggc
     8461 gaaacggcgc aggatctcgc tggcgtagac gatgcccgtc gtgcgggtgt tgccggcgcc
     8521 gttcagctgc acgaacttca catcgtgcaa cgggtggggc acgaggtggc ggctgacggc
     8581 gctcaatgtc gagccccagg cgatgccgac ggtcatcgac gagtcgagga actggccgag
     8641 catgcgcgcg gcggagaggg cgacccggtc cagacggtcg atgtcgctcg tgtggtcggg
     8701 gatcggcacg atatgcacgg ccacgccgta tcgctgatgg atcgcgcctt gcaggcggcc
     8761 ggtgtcttct tgcggcgagc ggatggtgac ctcgacgagg ccggactcgc gggcgaagcc
     8821 caagagccgc gagaccgacg agcgagacac ccgcatctcg tgggcgatcg cctccatggt
     8881 gaggtcttga aagaagtaca gtcgcgcggc acgcaaggcc tgggcgacgc gatcggacgt
     8941 cgggtgctgt tccggggagc tcaccgtcct atcatgcacg tttgtgcagt gtgcttgaac
     9001 tgaatcccgg caattcgcac aatggggctg tgcggccgct gccggccgag cgccctgtgc
     9061 gtcgaccggc gcgtgtcgga tcgtgctgtg ccggaccttg ggcggggcac gacgggtggc
     9121 gcccggccgt gcatgttggg aaggggcggc tctcatggca cgcaatatgg atcttcaccg
     9181 ttcgaacgtc gcggggctgc gtgaacggcc cgaggcgcag gtgctcgtga tcggcggcgg
     9241 catcaacggc ctcggcacgt tccgcgacct ggccctgcag ggaatcgatg ttgcgctcgt
     9301 cgagcgctcc gactacgcgt ccggcgcctc cagcgcctcg tcgcatatga tccacggcgg
     9361 cgtgcgctac ctcgagaacg gcgaattccg actcgttcgt gaggcggtgc acgagcgcaa
     9421 cgggctcttg aagatcgcgc cgcactacgt caagccgctg cgcaccacga tcccgatctt
     9481 tcgcacgttc tccggcatcg tcgccgcgcc gctgcgattc gtgacccaca agcagcgcag
     9541 cacggttgaa cgtggggcgt tgctcatcaa gctcggcttg acgatctacg actcgttctc
     9601 ccgtgacggc ggcaccatac cgaggcacgt cttccggcgt tccaaggcgg ccctgcgcga
     9661 cctgccccgc ctgaacccgg ccgtgaaata tgcggcgacg tactacgacg cctcggtgca
     9721 cgagccggaa cgcctcgcgc tcgacgtgct gcgtgacgcc cgtgcggccg cgggcccggc
     9781 ccgcgccgcg aactacgtgg ccgcggtcgg ggtcgcagac ggccgggtgc ggctgcgcga
     9841 cgagatcacg ggggaggagt tcgcgttcca cgccgacctg atcatcaacg cctccggccc
     9901 gtggaccgac ctgaccaacg gcgcactcgg ccgcacgacg aggctcatgg gcggtaccaa
     9961 aggctcgcat atcgtgctgg acagccccga gctcttggcg gcgtgtgccg gccgcgagct
    10021 cttcttcgag aactccgacg ggcgcatcgt cttgatctat ccgctgcgcg gcaaggtcat
    10081 ggtcggcacc accgacctgg aggccgatat cgccgggccg atccgctgca ccgacgccga
    10141 ggtccggtac ttcttcgacc tcatcgcgca cgtcttcccc gacgtgcggg tggacgagag
    10201 ccagatcgtc taccgctttg ccggcgtacg gcccctgccg gcacacgggg acacccagcc
    10261 ggggttcgtc tcccgggact accgcataga caccgaccgc ccggcgaacc ttcccggcac
    10321 gactgtgctc agcctcgtag gcggcaaatg gacgaccttc cgggcggtcg ccgagcgtct
    10381 caccgatacg gcgttgagcc ttttgggcga accgcggcgg gtcagcacga acggtctgcc
    10441 gatcggcggc gggcgagact acccggccga cggcgaggcg aggcggcggt ggatcgccga
    10501 acaccgtgac ggactctccc cggagcgtgc cgagcagctg ctgcgtcgct acggcacaaa
    10561 cgccgaggag gtcatcggct tcctcgccga gcacggcgac gacccgttgg ctgcgaccac
    10621 ggagttcacc gtgcaagagc tggagcacat ggtcttgcat gaggatgtcg tgcacgtgca
    10681 cgacgtgctt atgcgccgca ccagctcggc cttcgacggc gcggtcacct ctgcggtcat
    10741 cgaagagacg gccgcacacc tgcagacgtt gcttggctgg tcgggcggtc gcctggccga
    10801 cgaggttgcc gaaacgacgc gcatcctcgc cgacgagcac ggcgtgcgac tcgagcggcc
    10861 ggctgccccg gtcgggggga ggtgatcgcg ccgaccgacc catccccgtg cgccgacccg
    10921 gagcgtagcc tgtgcggcaa cggcgttcct caccacatcc gctacacaag accatcaaac
    10981 gacggagttc gataatcatg gatcaatacg tcatcgcgat cgatcagggc acgacctcgt
    11041 cgcgcgccat cgtcttcgac aagtccggct cgatcatctc ggtcggccag ctcgagcacg
    11101 agcagatctt cccgaaaccc gggtgggtgg agcacgatcc gcaggagatt tggcggaaca
    11161 ctcgcgaggt catcggccag gcgctctcca aggcgaacct cacacggcac aacattgcgg
    11221 cgatcggcat cacgaatcaa cgtgagacgg cggtcgtctg ggacaagaac acgggggagc
    11281 ccgtctataa cgcgattgtg tggcaggata cccgcacgca agacatcgtg gacgagctgg
    11341 cggccgacgg tggcgtcgag cggttcaagt cgatcgtcgg gctgccgttg gcaacctatt
    11401 tctccggtac gaagatcaaa tggatcctcg acaacgtgcc cggcgcccgc gaacgtgccg
    11461 aggcggggga tctctacttc ggcaacaccg acagctgggt cttgtggaac ctgaccggag
    11521 gggtgaacgg ctgcatccac gtcaccgacg tcacgaacgc cagccgcact ttgttcatgg
    11581 atctgaagac gctgcagtgg cgcgacgata tcctccacgt gttcggcgtg cctaagtcga
    11641 tgctgccgga gatccacagc tcctccgagg tctacggggt ggcggagccg aacagtcttc
    11701 tgcgggagac gccgatcgcg ggcatcctcg gcgaccagca ggcggcgacg ttcggccagg
    11761 ccgccttcga gaaaggcgag gcgaagaaca cctacggcac cgggaacttc ctcatcttca
    11821 acaccggtga ggagatcgtc gaatcgaaga acgggctcct gacgacaccc gcttacaagc
    11881 tcggggacgc caagccgcat tacgcgctcg aaggatcgat cgcggtgacc ggctccctcg
    11941 tccagtggct gcgcgacaac ctcgggatca tcgggtccgc ccccgagatc caggcgctcg
    12001 ccgagacggt ggaggataac ggcggcgcct acttcgtgcc ggcgttctca ggtcttttcg
    12061 ccccgtactg gcgctccgac gcgcgtggtg ctctcgtcgg tttgacgcgc ttcgtgaaca
    12121 agggccacct cgcgcgggcc gtgctcgaag cgacggcgta tcagaccagg gaggtcctcg
    12181 acgcggtcaa cgcggactcc ggagtgccct tgaccgagct gaaggtcgac ggcggcatga
    12241 cggcgaacaa tctcctcatg cagttccaag ccgacgtcat cggggtgccc gttgtgcgcc
    12301 cggtcgtcgc agagaccacc gcactcggtg ccgcctatgc ggccggcctg gcggtcgggt
    12361 tctgggagag cctggacgat ctgcgcggca actggcagga ggaccggcgc tgggagccgg
    12421 ccatggccga agctgagagt gagcgcctcc tgcgcaactg gaagaaggcg gtcaccaaga
    12481 ccttcgactg ggtcgacgcc gacacgcagt gacggtcggc ggcgcgacac cgtctgccgg
    12541 tgaggggtcg gggccttcgg gtcccggccc ctcaccgcgc gtggccgccg tctcctcggt
    12601 cgggaatatc gctcccgtgt gcgcgttgga aagggtatat gcaaacgtat atagttagag
    12661 tgcgtacgag agaatggaca cggccgtgac ccagagggac ccatccggga tgcagttcgg
    12721 catcttcacc gtcagcgatg tcacccgcga tccggtgacc ggcacgaccc cgagcgaggc
    12781 cgagcgcatc caggacacgc tgaccatcgc gcagcatgcc gaggaggtcg gcctggacgt
    12841 cttcgcgatc ggcgagcatc acaacccgcc gttctggtcg tcctcgccca ccacgaccct
    12901 ggccttcatc gcggcgcgga cccggcgtct gatcgtctcg acctcgacca cactcgtgac
    12961 cacggacgac ccggtcaaga tcgcggagga cttctccgtc ctccagcacg tcgccggcgg
    13021 ccgcgtcgac ctcatgctcg ggcggggtaa caccggtccc gtctatccct ggttcggtaa
    13081 ggacatccgt caggggcttg aattggccgt agagaactat gcactcctgc accggttgtg
    13141 gcgtgaagac gtcgtggact tctcgggcaa tttccgcaca cccctccagg ggttcacctc
    13201 ggtcccgcgg ccgttggacg gcgtcccgcc gttcgtgtgg cacggctcga tccgcacgcc
    13261 ggagatcgca gaacaagccg cctattacgg cgatgggttc ttcgcgaaca acatcttctg
    13321 gccggccgag cactaccaac gactcgtcga gctctaccgg cagcggttcg ctcactatga
    13381 gcacggcacg ccggagcaag cgatcgtcgg gctcggcggt caggcgttca tgcgcgagaa
    13441 ttctcaagac gcagtcaatg aattccggcc atatttcgat aatgcgccgg tttatggtca
    13501 tggcccttcc atggaggact tcacgaaaat gactcccctc acggtcggct cgcc
//
LOCUS       DASTNL010000189         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1251483, whole genome shotgun sequence.
ACCESSION   DASTNL010000189 DASTNL010000000
VERSION     DASTNL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251483"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2444
                     /gene="mfd"
                     /locus_tag="VFQ96_05835"
     CDS             <1..2444
                     /gene="mfd"
                     /locus_tag="VFQ96_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU0257344.1"
                     /translation="AEGPGLVDRALAVLTDHGLAARAVDAVPGRALEAGVAYVTKASV
                     EHGFELPEVRLAVIGEDEFYGRSVGYDARQAKKLASRRKKNVVDPLQLKAGDYVVHQT
                     HGIGRFVELTQRPVVVGGRGRQAVTSMREYVVLEYAASKRGRPADRLYVPTDQLDLLS
                     RYVGGEAPTLSKMGGSDWAAAKGRARKAVRDIAVELVKLYSARMASKGHAFSPDTPWQ
                     HELEDAFPFVETPDQLQTIDEVKADMERPIPMDRLVSGDVGFGKTEIAVRAAFKAVQD
                     GKQVIVLVPTTLLVRQHLETFQERFAGFPVHLRALSRFQSDKEARETLAGLADGTVDV
                     VIGTHRLLAKDLVVKDLGLVIIDEEQRFGVQHKDALKKLKTDVDVLAMSATPIPRTLE
                     MAVTGIREMSTLATPPEDRHPILTYVGPYSERQVAAAIRRELLREGQVFFVRNRVSSI
                     NRAASRLAELVPEARIAVAHGKLPEHVLEQVVVDFWERKFDVLVCTTIIETGLDIANA
                     NTIIIDRADKYGLSQLHQLRGRVGRGRERAYAYFLWDDTKPLSETAHDRLSTIAANNE
                     LGSGMQVALKDLELRGAGNLLGAEQAGHIAGVGFDLYLRMIGEAVDTFRGDVAEGQTE
                     LRLELPVDAHIPDAYVGSERLRLEAYQKLSTASAPAARSDAIDLVVDELTDRYGAPPE
                     PVQNLVAVSRLRRHAQKAGLGEVVAMGAKLRVGPVDLPDSIQVRLRRLYPGARLTSTA
                     HTLSVPLPVHGDDRPADAELIDWVSGFLTAIFPAEDEKRSGRGTAGTAGEAAPAGGAR
                     GADSVSAGDPAGRLG"
     gene            2599..3336
                     /locus_tag="VFQ96_05840"
     CDS             2599..3336
                     /locus_tag="VFQ96_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEU0257345.1"
                     /translation="MRGLVELMATLRGPGGCPWDQAQTHESLVRYLLEESHELVEAIE
                     AGGPDDLLEELGDVLYQVLFHADIAAHTAGEAFDLDDVADHMIAKMVGRHPHVFGDRR
                     AADAAAVTGFWEELKRAEKPERTSVLDGIPRSLPSLLLADKMLGRAASVGAGPRRGPG
                     RPAPSAAATSLAAADVPVDEGRLGDLLLTIVADARAAGLDAERALRTRLRALADEVVR
                     AEGAAVPGSSAARGPRGAEGAAGEMGE"
     gene            3341..4636
                     /gene="hisS"
                     /locus_tag="VFQ96_05845"
     CDS             3341..4636
                     /gene="hisS"
                     /locus_tag="VFQ96_05845"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU0257346.1"
                     /translation="MADAVQPPRGMRDFLPAEKAHRENVLAVIRGVYQAHGFSWIETP
                     VVEDVRRLHSGLGGDNEKLGFSVLRRGLDTDDLRRAADGGDPLLLADLGLRFDLTVPL
                     ARFYASHRAVLPSVFRSLQIAPVWRAERPQRGRFRQFTQCDIDVIGEPGPLAEAELMT
                     ATSAALGALGLTGCTIRVNDRRILDALLEHCGFAADRRAMTLVSLDKLDKIGAEGVLA
                     ELGAHDPEPARRLGAVLSGLEERLRAGIPLTAAAIGEVLPATVAPGVAAELDALARVV
                     APVAANVRLVFDPTLVRGMGYYTGTIFEISHPASGSSIGGGGRYDEMIGRFAGVAVPA
                     CGFSIGFERVVDLVARKARGGDTATVLVYEREVPHADLMRLKTALVAGGDRVRLARRT
                     KNLKGLLGKAAEDGYTTFALVDRETAGRGAAALETRPLA"
     gene            4740..>5891
                     /gene="eno"
                     /locus_tag="VFQ96_05850"
     CDS             4740..>5891
                     /gene="eno"
                     /locus_tag="VFQ96_05850"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0257347.1"
                     /translation="MAEIEAVGAREILDSRGNPTVEVEVLLDDGTVGRASVPSGASTG
                     AFEAYELRDGGERYLGKGVEKAVAAVLDELAPAVEGLDADEQRLVDHALIECDGTANK
                     SRLGANAILGVSLAVAKAAADSADLALFRYLGGPNAHVLPVPMMNVINGGAHADNNVD
                     VQEFMILPIGAPTFREALRWGAETYHSLKSLLKKRGLGTGLGDEGGFAPDLKSNRAAL
                     DLLVEAVTAAGFTPGTDVSLGLDAASSEFFADGHYTFEGREVASDFFTGYYRDLIAQY
                     PLITIEDPLAEDDWEGWSRLTGELGGTVQLVGDDLFVTNPARIADGVAKGAANSVLVK
                     VNQIGSLTETFDAVELAHRSGYTSVISHRSGETEDTTIADLAVATNCGQI"
BASE COUNT          927 a         2079 c         2041 g          844 t
ORIGIN      
        1 cggccgaagg ccccggcctc gttgaccggg cgctcgcggt cttgaccgac cacggcctgg
       61 ccgccagggc cgtcgacgcc gtgcccggtc gggcgctgga ggctggcgtc gcctatgtca
      121 cgaaagccag cgtggagcac ggcttcgagc tgcccgaggt gcggctcgcc gtgatcggcg
      181 aagacgagtt ctacggcaga agcgtgggct acgacgcgcg ccaggcgaag aagctggcct
      241 cccggcgcaa gaagaacgtc gtcgacccgt tgcagctgaa ggccggcgac tatgtcgtcc
      301 accagaccca cggcatcggc cgcttcgtcg agctgacgca gcggcccgtc gtggtcggcg
      361 gccgaggccg acaggccgtg acgtcgatgc gcgaatacgt cgttctcgaa tacgccgcct
      421 cgaagcgtgg ccggccggcg gaccggcttt acgtgcccac cgaccagctc gacctgcttt
      481 cccggtatgt cggcggtgag gcgccgacac tgtcgaagat gggcggcagc gactgggcgg
      541 ccgccaaggg ccgtgcccgc aaggcggtgc gcgacatcgc cgtcgaactg gtcaagctct
      601 actcggcacg gatggcctcc aagggccacg ccttcagccc cgacacgccg tggcagcatg
      661 agctggaaga cgcgttcccg ttcgtcgaga cccccgacca gctgcaaacc atcgatgagg
      721 tcaaggccga catggaacgg cctatcccga tggaccggtt ggtctccggc gacgtgggtt
      781 tcggcaagac ggagatcgcg gtgcgtgccg cgttcaaggc ggtccaagac ggcaagcagg
      841 tcatcgtgct cgtgccgacg acgctcctcg tgcgccaaca cctcgagacg ttccaggaac
      901 gcttcgccgg gtttcccgtg catctgcgcg ccctcagccg gttccaaagc gacaaggagg
      961 cgcgcgagac gctcgccggg ctggcggacg gcaccgtcga tgtcgtcatc ggaacccacc
     1021 gcctgctggc gaaagacctc gtcgtcaagg atctcggcct cgtcatcatc gacgaagagc
     1081 agcgtttcgg cgtgcagcat aaagacgcgc tgaagaaact caagaccgac gtcgatgtcc
     1141 tggcgatgag cgccacgccg attccccgca ccctggagat ggcggtcacg ggcatccgcg
     1201 agatgtcgac gctcgcgacg ccgcccgagg atcgccatcc gatcctcacc tacgtcggcc
     1261 cgtattcgga gcggcaggtg gccgccgcga tccgccgcga gctcttgcgc gaaggccagg
     1321 tgttcttcgt gcgcaaccgg gtctccagca tcaaccgggc ggcctccagg ctggccgagc
     1381 tcgtccccga ggcgcggatc gccgtggccc acggcaagct tccggagcat gtgctcgagc
     1441 aggtcgtcgt cgacttctgg gaacgcaagt tcgacgtcct cgtctgcacg acgatcatcg
     1501 agacgggtct cgacatcgcc aacgcgaaca cgatcatcat cgaccgcgcc gacaagtacg
     1561 gcctcagcca actgcaccag ctgcgcggca gggtgggccg tggccgggaa cgcgcctacg
     1621 cctactttct ctgggacgac acgaaaccgc tctctgagac cgcgcacgac aggctctcca
     1681 cgatcgccgc gaacaacgag ctcggcagcg gcatgcaggt cgcgttgaaa gacctggagc
     1741 tgcgcggcgc aggcaatctc ttgggtgccg aacaggcggg ccacatcgcg ggcgttggct
     1801 tcgaccttta tttgcggatg atcggcgagg ccgtcgacac gttccgcggc gacgtcgccg
     1861 aggggcagac cgaactgcgg ctcgagctgc ccgtcgatgc gcacattccc gacgcgtatg
     1921 tcggcagcga gcggctgcgg ctcgaggcgt accagaagct gtctacggcc agcgcacccg
     1981 ccgcccgctc cgacgcgatc gacctcgtcg tcgacgagct gaccgaccgc tatggcgcgc
     2041 cgcccgagcc ggtgcagaac ctcgtcgccg tctcccggct gcgccggcac gcccagaagg
     2101 cggggcttgg cgaggtggtc gccatggggg cgaagctgcg ggtggggccg gtcgacctgc
     2161 ccgactcgat ccaggtgcgc ctcaggcggc tctaccccgg cgcgaggctc acctcgacgg
     2221 cgcacaccct ctcggtcccc ctgcccgtcc acggcgacga ccggccggcc gatgcggagc
     2281 tgatcgactg ggtctccggc ttcttgacgg cgatcttccc ggcagaagac gagaagaggt
     2341 ccggccgggg caccgccgga acggcggggg aggcggcgcc cgccggcggc gcgcgaggcg
     2401 ccgactccgt ttccgccggc gaccctgcgg gtcggcttgg ctgacccggg gccgggaggc
     2461 gacgcggcac gcgaggggag gaggggcgcg gcgatgggcc atggcgatca cggtgacgag
     2521 gccagagaac cttcccagcc gcaggaggcc cgggatcgag ggcccgggcc ccgtccacac
     2581 ccgccggccg aaggcggcgt gcgcggcctc gtcgagttga tggcgaccct gcgcggcccc
     2641 ggcggttgcc cgtgggacca ggcgcagacc cacgagtccc tcgtgcgcta cctccttgag
     2701 gagagccacg aacttgtcga ggcgatcgag gccggcgggc cggacgacct cctggaagag
     2761 ctcggtgacg tgctttacca ggtcttgttc cacgccgaca tcgcggcgca taccgcgggc
     2821 gaggccttcg acctcgacga cgtggcggac cacatgatcg ccaagatggt gggtcggcat
     2881 ccgcacgtct tcggcgaccg gcgggcggcc gatgccgcgg cggtcaccgg cttctgggag
     2941 gagctcaagc gggccgagaa gcccgaacgc acgagcgtgc tcgatggcat accccgttcc
     3001 ctgccctctt tgcttctcgc cgacaagatg ctcggccggg ccgcctctgt cggcgccggc
     3061 ccgcgccgcg gccccggccg gcccgcgccg tcggccgccg ccacctcgtt ggccgcggcc
     3121 gatgtgccgg tcgacgaggg gcgcctgggc gacctgctgc tcacgatcgt cgccgatgct
     3181 cgcgcagcgg gcctcgacgc ggagcgggcc ttgcgcacga ggctccgggc gctcgccgac
     3241 gaggttgttc gcgccgaagg cgcggccgtg ccgggttcct cggccgcccg gggcccccgc
     3301 ggcgccgagg gcgccgccgg cgagatggga gaatgacgcc atggccgacg ctgtgcaacc
     3361 accccggggc atgcgggatt tccttcccgc cgagaaggcg caccgcgaga atgtgttggc
     3421 cgtgatccgc ggcgtctatc aggcgcatgg tttctcctgg atcgagaccc ccgtcgtcga
     3481 agacgtgaga cgcctgcact ccggcctcgg cggcgacaac gagaagctcg gcttcagcgt
     3541 tttgcgtcgc ggcctcgaca ccgacgacct gcgcagggca gccgacggcg gcgacccgct
     3601 tcttctcgcc gatctggggc tgcgcttcga cctgaccgtg ccgctcgccc ggttctacgc
     3661 ctcgcatcgt gccgtgctgc cgagtgtgtt ccgctcgttg cagatcgccc cggtgtggcg
     3721 tgccgaacgg ccgcaacgcg gccggttccg ccagttcacg caatgcgaca tcgacgtcat
     3781 cggcgagccc gggccgttgg cggaggcaga gctcatgacg gccacgagcg cggcgcttgg
     3841 agccctcggc ctgaccgggt gcacgatccg cgtcaacgac cgccgcatcc tcgacgcgct
     3901 cctggagcac tgcgggttcg ctgccgaccg ccgcgccatg acgctcgtct cactcgacaa
     3961 gctcgacaag atcggggcgg agggtgttct cgccgaactg ggcgcccacg acccggagcc
     4021 ggcacgccgc ctgggtgcag tgctctccgg cctcgaagag cggttgcgcg cggggattcc
     4081 cctcacagcc gccgcgatcg gcgaggtgct gcccgccacg gtcgcccccg gcgtcgcggc
     4141 cgagctcgac gccctcgccc gcgtcgtcgc gcccgttgct gcgaacgtac ggctcgtgtt
     4201 cgaccccacc ctggtgcgcg gcatggggta ctacacgggc acgatcttcg agatctcgca
     4261 ccccgcctcc ggcagttcca tcggcggcgg cgggcgatac gacgagatga tcggccgttt
     4321 cgcgggcgtc gccgtgccgg cgtgcgggtt ctcgatcggg ttcgaacgcg tcgtcgacct
     4381 cgtcgcccgc aaggcccgcg gcggcgacac ggcgacggtg ctcgtctacg agcgagaggt
     4441 gccgcacgcc gacctcatga ggctgaagac ggcacttgtc gccggcggcg atcgggtacg
     4501 gttggccagg cgcacgaaga acctgaaggg gctgctcggc aaggcggccg aagacggata
     4561 cacgacattc gccctggtcg accgcgagac ggcggggcgc ggggcggcag ccctcgaaac
     4621 ccgcccgctg gcctgaaccg ccgcgaggcc ccggccggcc gatgccgcac aggaggcccg
     4681 gcacacgggc gccgcacgca agatcaccga caacacacaa gatcaccaag gagacgatcg
     4741 tggcagaaat cgaagccgtc ggagcacggg agatcctcga ctcgagagga aaccccacgg
     4801 tcgaggtcga ggtcttgctc gacgatggca ccgtgggccg tgcctccgtg ccctccgggg
     4861 catccaccgg ggccttcgag gcatatgagc tgcgcgacgg cggcgaacgc tacctcggca
     4921 aaggtgtcga gaaggcggtc gcggccgtcc tcgacgagct tgcgcccgcc gtggagggcc
     4981 tcgacgccga tgagcagcgc ctcgtcgatc acgcattgat cgaatgcgac ggaacggcga
     5041 acaagagcag gctcggcgcg aacgcgatac tcggcgtcag cctcgccgtg gccaaggccg
     5101 ccgccgacag cgccgacctc gccctcttcc gttacctcgg cggtccgaac gcccacgtgc
     5161 tgcccgtgcc catgatgaac gtcatcaacg gcggggcaca cgccgacaac aacgtcgacg
     5221 tgcaagagtt catgatcctg ccgatcggtg cccccacctt ccgtgaggcg ctgcgctggg
     5281 gtgccgagac ctaccacagc ctgaagagcc tcttgaagaa gcgtggcctt ggcacagggc
     5341 tcggcgacga gggcgggttc gcgcccgacc tgaagagcaa ccgggcggcg ctcgacctgc
     5401 tcgtcgaggc ggtgacggcg gcgggattca cgccgggcac cgacgtctct ttgggcctcg
     5461 acgccgcgtc gagcgagttc ttcgccgacg ggcactacac gttcgagggt cgtgaggtcg
     5521 cctccgactt cttcacgggc tactaccgtg acttgatcgc ccaatacccg ctcatcacca
     5581 tcgaagatcc gctggccgag gatgactggg agggctggag ccgcctgacc ggcgagctgg
     5641 gcggcaccgt gcaacttgtc ggcgacgacc tcttcgtcac caacccggcc cgcatcgccg
     5701 acggcgtggc gaagggcgcc gcgaactcgg tgctcgtcaa ggtgaaccag atcggcagcc
     5761 tcaccgagac gttcgacgcg gtcgagctcg cgcaccgctc gggttacacg agcgtcatct
     5821 cgcaccgctc cggcgagacg gaagacacga cgatcgccga tctcgccgtc gcgaccaact
     5881 gcggccagat c
//
LOCUS       DASTNL010000190        20113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_696645, whole genome shotgun sequence.
ACCESSION   DASTNL010000190 DASTNL010000000
VERSION     DASTNL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 20113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20113
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696645"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..804
                     /locus_tag="VFQ96_05855"
     CDS             430..804
                     /locus_tag="VFQ96_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0257348.1"
                     /translation="MRILLAPSSAMPLYEQIKDQVRAAVYGGGLNAGDMLPSLRRLAA
                     DLRVSIMTVTRAYNDLAAEGLVRNEQGRGFVVRPVDSDRAKKALEERLRAALRDFVGA
                     ARAARLTRSEAAGLLDTEWDTP"
     gene            801..1688
                     /locus_tag="VFQ96_05860"
     CDS             801..1688
                     /locus_tag="VFQ96_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257349.1"
                     /translation="MNEKTSATPLALRAEGLTVRAGDFLLDAVDVEVPTGYVVGMVGP
                     NGAGKTTTIKAILGMVGRSSGFVELFGEPASRQAARNAQIGVVLDRPFFSPDWRVASV
                     GTRVGRFYPTWDADRFAGLLREFRLPARTSVGALSRGQGMRLSFAVALCHDPRLLILD
                     EPTGGLDPVARAELVEGLREFMVDENHSVLFSTHITSDLDGLADFIQVLDGGHSTYRG
                     TLDDLHAQFALVQGVGDPGRDALRAVLGLRRARDRFEGMIRVADSHLFGRDAELSAAT
                     TDDIVVHLVRAGHDNEKGL"
     gene            1736..2332
                     /locus_tag="VFQ96_05865"
     CDS             1736..2332
                     /locus_tag="VFQ96_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HEU0257350.1"
                     /translation="MRLLAVAAMLIFFGIDMGPAFAIPLAAVACVLIASDLFLMDERG
                     RLDILYATLPVGRTTVVLGRYTTVLLEFLVAAVIGNTVAMLDVDFGRAPLGPGMLVMM
                     NVVGFVIVAVGLAVQLPAFFALGYTKARFVGLVPPVVIAVAGLSVGRKADILQGLAAI
                     VRGRGVSVPLEAAAVAGCVIVLAASVLAARALYVRRRL"
     gene            complement(2433..3173)
                     /locus_tag="VFQ96_05870"
     CDS             complement(2433..3173)
                     /locus_tag="VFQ96_05870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0257351.1"
                     /translation="MKEEKITVKTADGDCPVYVFAPDDGSRHPAAIMYTDAFGIRPAS
                     LAMGRRLASHGYVVLVPDIFYRAGSYGPIDPHEIYSTPNGFDSVRPLMASTDTRRATA
                     DSGRLLDYLDTRDDTSGTGVGVTGYCMGGTLAFTAGGSYPDRIAAVATFHTARLITDD
                     ELSPSLVATAMKARVLVAGADHDPGYPPEMAQELDRLLGEAGVERTLEVYPEARHGFT
                     QTDSPVYDEQAAERHWSELVGLFDSTLV"
     gene            3462..4754
                     /locus_tag="VFQ96_05875"
     CDS             3462..4754
                     /locus_tag="VFQ96_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257352.1"
                     /translation="MSAFFAKAWLLLLPAWAGRNVKVILAARVGMSVGRSVSSVVTAL
                     YLATIGFSGVEIGALFVAVTVASALMSTCIGLLADRWGRKPFLVVVPLIAAAAGVIFA
                     VTRVTAVLFLAAAVGSFGRGAGAGGGSVGPYRPAESAIVAETVPARHRAAAFARIASA
                     SSLGALVGGLTAQLVHVHAHMSPAEATAAYRPAFLMAAALAVVAGLLALLLREPERAP
                     RQSGRAPLRWPRRSWPALWRFWITNATNGAGAGLLGPFVSYWFFRRYGATPAQIGVLF
                     AVVNFFSLASTLASARVAARLGTVRAIVTVRAIGGVLLVPMVLAPSFLLAGGIYVVRM
                     MVQRVGLPLRQSFTQDMADPGERASVAALSNLPAQGTMAAGQAAGGWLFDEASLAAPF
                     TLAAVFQVANAALYWLLFSRRPPRPRAAGAADTRSRRA"
     gene            complement(4759..6921)
                     /locus_tag="VFQ96_05880"
     CDS             complement(4759..6921)
                     /locus_tag="VFQ96_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU0257353.1"
                     /translation="MAEHTHHDDMAPRHGASAPARGAGHASHTAPPERSGHADHAEHG
                     GHGGHAARFRRLFWVMLILATPTVGFSGMFASIVGYPLPGGAWATWVSPVVGTAMYVW
                     GGLPFLSGAVGEMRARRPGMMLLIALAISVAFLASWGSVLGLVDAHMDFWWELALLIV
                     IMLLGHWVEMRSLAQTTSALDSLAALLPDVAWRVDGEATVAVSPANLTVGDLVVVRPG
                     ARVPADGRVVSGAASMDESMITGESRTVRRGPGDAVVAGTVATDSGLRIRVSAVGEDT
                     ALAGIARLVAEAQESSSRAQRIADRAAGWLFWFALGVGVVTAVVWASLGSPDDAAMRT
                     ITVLVIACPHALGLAIPLVVSIATERAAHAGVLVKDRLALETMRRVDTVLFDKTGTLT
                     KGRPVVTDLSADPAYGDEGLLSLAAAAEADSEHPLARAIVAAARARGLDVPAASDFSS
                     SPAVGVSALVRGRLVQVGGARLPVGDDPAVRDDARAWHEAGATVLHVLVDGDPAGALR
                     LEDELREESSAAVDALHARGIRVVMITGDAHPVAAYAAQALGVDRFFAGVRPEDKAAK
                     VAQLRAEGRRVAMVGDGVNDAPALAAADVGVAIGAGTDVAVGSAGVILAGDDPRAVVS
                     MIELSRAGYRKMAQNLWWAAGYNLVSVPLAAGVLAPVGFVLPMSVGAILMSVSTLVVA
                     VNAQLLRRLDLGPDVVAGRAQGRDGAPGPRRDAVRVAR"
     gene            complement(7018..8058)
                     /locus_tag="VFQ96_05885"
     CDS             complement(7018..8058)
                     /locus_tag="VFQ96_05885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0257354.1"
                     /translation="MSTVKAYAAPAASSPLVPTTIERREVGPHDVLIDIKFVGICHSD
                     IHTVRGDWGAQVYPLTPGHEIAGIVAAVGTAVTKHAVGDRVGVGCMVDSCGECVNCLA
                     GNEQYCLAGNIGTYGVADHDGNITQGGYSTQIVVTEDFVLKIPAGVGLDEAAPLLCAG
                     ITTYSPLHHWAAGPGKSVAVVGLGGLGHMAVKIAHAMGAEVTVLSHTSGKEADARRLG
                     ADRFFATSDPATFTRLANTFHLIVNTVSAAIDINSYLDLLALDGALVNVGAPADPLSV
                     DVGRLLDMRRAFAGSSIGGIRETQEMLNFCAEHHLGADIEVISADQINEAYDRVLASD
                     VRYRFVIDTSTL"
     gene            8128..8424
                     /locus_tag="VFQ96_05890"
     CDS             8128..8424
                     /locus_tag="VFQ96_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU0257355.1"
                     /translation="MPLRHIVSWRLAAGPVRARLAAEIRERLLTLPSQVPEIRSYEVG
                     VDDEAAADGFDVVLVAEFDDRQALARYAAHPAHQAVAEFIRSAAEARSAVNYRV"
     gene            complement(8524..8599)
                     /locus_tag="VFQ96_05895"
     tRNA            complement(8524..8599)
                     /locus_tag="VFQ96_05895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8564..8566),aa:His,seq:gtg)
     gene            complement(8633..9253)
                     /gene="orn"
                     /locus_tag="VFQ96_05900"
     CDS             complement(8633..9253)
                     /gene="orn"
                     /locus_tag="VFQ96_05900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEU0257356.1"
                     /translation="MSTSPDRLVWIDCEMTGLDPEKDELVEVAVIVTDFALTVLDAGL
                     DLIIKPGDAALAHMDDFVRAMHTTSGLLDEIPTGLALADAETQVIDYIDRFVPHDKQA
                     PLAGNTIGTDRMFLVRYMPHLDARLHYRNVDVSSIKELARRWYPRVYFNAPEKHGGHR
                     ALADIRESIRELDYYRRVVFVADPGPDTDTVKSVAATVTDEWASRM"
     gene            9333..9593
                     /locus_tag="VFQ96_05905"
     CDS             9333..9593
                     /locus_tag="VFQ96_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0257357.1"
                     /translation="MQFELYTSAFCGPCRGARQVLADAERLVPRIHVREFDVVRDEAR
                     AKEQDIRSTPTIIVRDGTGRQVFRAQGVPRLGQVLVAAASAL"
     gene            complement(9673..9745)
                     /locus_tag="VFQ96_05910"
     tRNA            complement(9673..9745)
                     /locus_tag="VFQ96_05910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9710..9712),aa:Arg,seq:tct)
     gene            complement(9812..11218)
                     /locus_tag="VFQ96_05915"
     CDS             complement(9812..11218)
                     /locus_tag="VFQ96_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257358.1"
                     /translation="MTPPHVVDPAITAAIPIVAERPPWSEMFVALKVRNYQLFASSNL
                     VQNTGNWMQRIAMDWLVLQLTGSPTAVGITMAMQFTPMIFLGLFGGIVADRFPKRNLL
                     VGTQSAAALLAALMAVLTLTGIVTVWDIWAVSALLGVVTVVDNPARQVFVNELVGPTY
                     LRNAISINSSIFQLGGLVGPALAGVLIAVIGGGWMFTVNAVGCALVVTNLFRIRAGEL
                     YAAPVVPRGKGQLVEGLAYVLRKPPILWTIVMMALVAVFAQSMPVFLTSFANDVYHVG
                     AGGYGMFNALVACGALAGALLSTRRATVRLRLTVSGGAVVGGTQLVAGLMPDEWSFVA
                     VLVSFGLANLLFITAANQLVQMSSNMRVRGRVMALYILVWLGGQAVGGPVSGWAVETF
                     GPHLAMALTGSVPLVCAGVIAASLARRGRLRVRLTLHHAVPRLGIVRRESRLAVSHPQ
                     SSPAGRRHTRRLRRRRPA"
     gene            11336..12250
                     /locus_tag="VFQ96_05920"
     CDS             11336..12250
                     /locus_tag="VFQ96_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0257359.1"
                     /translation="MDPNDVTAGLQAAVRPFDTVLLRTFLAVADTESFTRAAEQLGIS
                     QPTVSQHVRRLEEAAGSVLVTRDTRHVHLTEKGEAMAGFSRRILAIHAEAVSFFRGSQ
                     MRGRLRFGTADDLAITQLPRILRHFRQLHPQIELELTVSQSAALLRRLKAGQLDLVFI
                     KQEPDEATGIPVRRDVLVWMGQDKTLIDENDAVPLVTYQTPSLSRRTAIRALESVGRR
                     WRITCNIKDVNSTLAAVRAGLGIAVFPQSLIPDDLVKVTRRFGLPELGDVMFSLLKSP
                     TAPAAPSAALTEAIIRRDLRRRGHAVRA"
     gene            12263..13069
                     /gene="nadE"
                     /locus_tag="VFQ96_05925"
     CDS             12263..13069
                     /gene="nadE"
                     /locus_tag="VFQ96_05925"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU0257360.1"
                     /translation="MRVLQSEIIRALHVEGTIDPHDEILSRVRFLADYVGRTGAAGLV
                     LGISGGQDSSLAGRLCQLAAEQLRDGGREARFVAVRLPYRRQHDDDDARLALDFIRPD
                     EQVEIDIAGPVDSLVAACAAAGRPLSDFDRGNVKARVRMAVQYAVGGADKMLVVGTDH
                     AAEAVTGFFTKYGDGAADLTPLTGLTKRQGAALLAALGAPERLWQKTPTADLLDGRPG
                     QPDEAELGITYQAIDDYLEGREVQPADAEAIERRYRSSAHKRRLPATPFD"
     gene            complement(13132..13896)
                     /locus_tag="VFQ96_05930"
     CDS             complement(13132..13896)
                     /locus_tag="VFQ96_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257361.1"
                     /translation="MQWLSDFTAWLTSDDTQPVVFTAIVLALAVIVAGLLAAWISRGA
                     LQSVLTRTERQQKASAITAIVDAATEASVWNSLTPQEQILSDRAVGQADTLIRLMPVR
                     GSGTAANWAAHQLAEMKRHSATYSYQLDPAVAELRDRLIEWQYHPRRIHKTFQEDLDR
                     WSFEYQAEKEDREAARRQNDPAANTSRPDPAGPAAEGLRAEDLEDDEALTKTVATPTT
                     PGAPEAARQADGETAPASELVVDQNPGMLPTGPGAD"
     gene            complement(13988..14992)
                     /locus_tag="VFQ96_05935"
     CDS             complement(13988..14992)
                     /locus_tag="VFQ96_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0257362.1"
                     /translation="MTYAPDAHRYDTMTYRRSGRSGLLLPAVSLGIWHNFGPSRSFET
                     QRAIVRRAFDLGVTHIDAANNYGPPPGAAEETLGRLVAGDFRHHRDELILSTKAGYLM
                     WPGPYGDHGSRKYLLASLDQSLSRLGVDYVDVFYHHRPDPETPLEETMGALADAVRLG
                     KALYVGVSSYSPEETREAAAILRSMGLPLTIHQPRYSLFDRQVEHGLLDAVTEVGAGT
                     IVFSPLAQGLLTNRYLDGAIPADSRAATSRFLDPSRIDDAYLARARALSDIARRRGQS
                     LAQLALRWVLRRPEVTSALVGASSVAQLEQNVAALEGPDLTAAELAEIEPLAVDLPSH
                     "
     gene            15039..15557
                     /gene="msrA"
                     /locus_tag="VFQ96_05940"
     CDS             15039..15557
                     /gene="msrA"
                     /locus_tag="VFQ96_05940"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0257363.1"
                     /translation="MTTFHLAGGCFWCLDAVYRTLRGVTDVTSGYTGGTVAHPSYELV
                     CAGSTGHAETARVTFDETQIPAETVLDVFFTMHDPTQLNRQDGDIGTQYRSAMFYTDD
                     GQRALFEAARDRAAGWWGSTVTAIEPLTTFYEAEGYHQDFYAKNPGQGYCMVVTRPKV
                     LKIRKRFAQYAR"
     gene            15826..16302
                     /locus_tag="VFQ96_05945"
     CDS             15826..16302
                     /locus_tag="VFQ96_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0257364.1"
                     /translation="MRTPDILAVTGTVGTDPERRVGESGIDMTSFRLATNHSRFDKAT
                     GQWVEQPANWYTVSAYRGLASHVVASVRKGERVTVVGRLRIARWQSGERSGTSVEIVA
                     ETVGLDLTFAPASRLAPSEGPVARAAASSAAPPAPPQTPALATPREGFLPEPEPVF"
     gene            16390..18069
                     /gene="ettA"
                     /locus_tag="VFQ96_05950"
     CDS             16390..18069
                     /gene="ettA"
                     /locus_tag="VFQ96_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU0257365.1"
                     /translation="MEYIYSMVRARKAVGDKVILDDVTMAFLPGAKIGVVGPNGAGKS
                     TILKIMAGLERPSNGDAELSPGYSVGILLQEPELDDSATVLENVQQGVGELKGKIDRF
                     NEISAALADPDADYDTLLAEMGGLQEAIDAAGGWDLDNQLDQAMDALRCPPPDTPVSV
                     LSGGERRRVALCRLLLQKPDLLLLDEPTNHLDAESVQWLEAHLAQYHGAVLAVTHDRY
                     FLDHVAEWICEVDRGRLYPYEGNYSTYLEKKHERLQVQGKKDAKLAKRLADELEWVRS
                     GAKGRQAKSKARLARYEEMAAEAERGRPLDFEEIQIPAGPRMGSLVIEAKDLVKGFDG
                     RTLIDGLSFSLPRNGIVGVIGPNGVGKTTLFKTIVGLESLDAGELVIGDSVQISYVDQ
                     LRAGIDPDKTLWEVVSGGLDYIRVGKTEMPSRAYVSTFGFKGPDQQKRAGVLSGGERS
                     RLNLALTLTQGGNLLLLDEPTNDLDVETLSSLENALLEFPGCAVVVTHDRWFLDRVAT
                     HILAYEGTEENPAAWYWFEGNFEAYEANKIERLGPDAARPHRSVYRRLTRG"
     gene            18066..18566
                     /locus_tag="VFQ96_05955"
     CDS             18066..18566
                     /locus_tag="VFQ96_05955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0257366.1"
                     /translation="MRRLHVPVRLRWSDVDAYGHVNNAEMFRLLEEARIQAFWAPDLA
                     PGLREPAEPGEGASETTRVLAGGADADTLSFIAKHTIEYLTPIPYMSEPIDVQLWLGR
                     LGGASLDVCYEAHPPRGLPEPVYVRASTTIVLVDAATRRPRRLTQRERAAWGPYLGDP
                     VTFNRH"
     gene            complement(18587..19435)
                     /locus_tag="VFQ96_05960"
     CDS             complement(18587..19435)
                     /locus_tag="VFQ96_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEU0257367.1"
                     /translation="MLDVLTLTDAGARTHEDIFTGRSVWMPAGRVFGGQVLAQSMIAA
                     CHTVPPERVVHSLHGYFLRPGDTALPISFAVDRIHDGRSFSARRVQAYQRGVPILSMI
                     TSFQTQDEGFDHQLDMPAGLPRPEELSGDDASLARLTDPVTRHWAQGRAFEMRHVSSP
                     VYLKVRGERVAHQCVWFKTRSPLPDDENLHRAALVYASDYSILESVFRRHGVSWSDRD
                     AKIASLDHAMWWHRFGRVDRWLLYAQESPSATGGRGFATGRIYTRDGILLASVAQEGM
                     IRVGGR"
     gene            complement(19618..>20113)
                     /locus_tag="VFQ96_05965"
     CDS             complement(19618..>20113)
                     /locus_tag="VFQ96_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HEU0257368.1"
                     /translation="DVNGTLWFVRNGEILRVGNMSHGWSRVILDLSVPLSADLDEVKS
                     ALMAEANKLATTPKWRARILRQAELWGLESIADNALVIRIVLRTRAGAKDDVSRELRA
                     RVKETMDALGVDIPTLATVVASGFDGAMSVYGLHPPRTKPLNVVAERHTRRRRNRPPD
                     EPGR"
BASE COUNT         3038 a         6809 c         7056 g         3210 t
ORIGIN      
        1 cccaagaggt cggggtcgcc atcgtcggaa aggtcttggg tcaggatcgc cacgttgtcg
       61 aggcgttcga cgatgccctc cggcagcgtg tcgaagacct ctccgaccag cgcgtcgaac
      121 tccttggccg acaggtcgat cacggcgctc ctcacctcgt cgtctgcatc agagactacc
      181 gaccctcggg gcggggtgcc ggcacgcccg gtagactgcc ctgcgttccg tgtgtaggcg
      241 gcccttgggc agggcggttg ctcacccgac gcgacagaat gcgccggtac ggggccccag
      301 atgggtccgg cacggaaggg cccgccggct agatcgcctt ccggcgggcc cgttcttttc
      361 ccgattatgc ggcgtgcgcg cggcctggta ctgtactgct gtacttatac agttgacaca
      421 aggttggcga tgcggatcct gctggcaccc tcgtccgcca tgccgctgta cgagcagatc
      481 aaggatcaag ttcgtgcggc ggtctatggc ggcggcctga acgcgggcga catgctgccg
      541 tcgttgcggc gcctcgccgc cgaccttcgg gtgagcatca tgacggtgac ccgggcatac
      601 aacgacctcg ccgcagaagg gctcgtccgc aacgagcagg gacggggctt cgtggtgcgg
      661 ccggtggatt ccgaccgggc gaagaaggcc ttggaagaac ggctgcgggc cgcgctgcgg
      721 gatttcgtcg gcgcggcccg ggcggcccgc ctgacgcgga gtgaggcggc cgggctactg
      781 gacacagaat gggatacgcc gtgaacgaga agacatcagc gaccccgctt gcgctgcgcg
      841 ccgagggttt gacggtgcgg gccggagact ttctcctcga cgcggtcgac gtcgaggtgc
      901 cgaccgggta tgtcgtcggc atggtcggcc cgaacggggc cggcaagacg accaccatca
      961 aggcgatcct gggcatggtg ggtcgaagct ctggctttgt ggagctgttc ggggagccgg
     1021 cctctcgcca ggccgcccga aacgcccaga tcggcgtcgt cttggaccgc ccgttctttt
     1081 cgcctgactg gcgggtcgcc agcgtcggca cccgggtggg caggttttat cccacctggg
     1141 atgccgaccg gttcgccggg cttcttcgag agttccggct gccggcgcga acgagcgtcg
     1201 gggcgctctc gcgcggtcag ggtatgaggc tctcgttcgc cgtcgcgctc tgccacgacc
     1261 cgcggcttct gatcttggac gagccgaccg gcgggctcga tccggtcgcc cgggcggagc
     1321 tcgtcgaggg cctgcgagag ttcatggtcg acgagaatca cagcgtgctc ttctcgaccc
     1381 acatcacctc cgacctcgac gggctcgccg acttcatcca ggtgctcgac ggtgggcaca
     1441 gcacctatcg cggcacgctc gacgacctgc atgcgcagtt cgctctcgtg cagggggtcg
     1501 gggatcccgg ccgggatgcc ctgcgggccg tcctcggcct gcgccgggcc cgcgacaggt
     1561 tcgaagggat gatccgtgtc gccgactcgc atcttttcgg acgggatgcg gagctttccg
     1621 ccgcgacgac cgacgacatc gtcgtccacc tcgtgcgcgc cggtcacgac aacgagaaag
     1681 gcctgtgatg atccatttcc tcgcccttga cctgcacggt cttgtggggc ggcgcatgcg
     1741 ccttctcgcc gtggccgcca tgctgatctt cttcgggatc gatatggggc ccgccttcgc
     1801 gatcccgctg gcagccgtag cctgcgtgct gatcgcgtcc gacttgttct tgatggatga
     1861 gcgaggccgc ctcgacatcc tgtacgcgac gctgccggtc ggccgcacga cggtcgttct
     1921 cggccgctac accaccgtgc tgctggaatt cctggtggcg gccgttatcg gcaacacggt
     1981 tgccatgctc gacgtcgact tcgggcgcgc cccgctcggc ccggggatgc tcgtgatgat
     2041 gaacgtcgtc ggcttcgtca tcgtcgccgt gggcttggcg gtccagctgc cggcgttctt
     2101 cgcgctcggc tacaccaagg cgcgtttcgt aggcctcgtc ccgccggtcg tgatcgccgt
     2161 ggccgggttg agcgtgggca gaaaggccga catcctccag gggctggcgg ccatcgtccg
     2221 ggggcgtggc gtgagcgtgc cgctcgaagc cgccgccgtg gcaggctgcg tgatcgtgct
     2281 ggccgcctcc gtactggcgg cacgcgcgct gtatgtgcgc cggcgcctct gagcgcccga
     2341 ggccggcgcg gcatcacgaa aacgctcggt cccctcgccg aacggctcct gccggcacga
     2401 cgggaggcgg ggccgccgcc gagaggtgcg acttatacga gtgtggaatc gaagaggccg
     2461 acgagctcag accaatgccg ctctgccgcc tgctcgtcgt agacgggcga gtcggtctga
     2521 gtgaacccgt gccgcgcctc cggatacacc tccagcgtgc gctcgacgcc cgcctcaccc
     2581 agtagccgat cgagctcctg cgccatttcg ggcgggtacc ccgggtcgtg gtccgcaccg
     2641 gcgacgagca cacgtgcctt catcgccgtc gcgacgaggc tcgggctgag ctcgtcgtcg
     2701 gtgatgaggc gtgccgtgtg gaaggtggcg acggccgcga tccggtcggg gtagctgccg
     2761 ccggccgtga acgccagtgt gccgcccatg cagtacccgg tcacgccgac ccccgttccc
     2821 gaggtgtcgt cacgggtgtc gaggtagtcg aggagtcggc cactgtcggc cgtggcgcgc
     2881 cgggtgtcgg tggaggccat caacgggcgc accgagtcga agccgttcgg cgtcgaatag
     2941 atctcatgcg ggtcgatcgg cccgtacgag ccggcccggt agaagatgtc gggcacgagc
     3001 acgacgtagc cgtgagaggc caggcgccgg cccatggcca acgacgccgg gcggatgccg
     3061 aacgcgtccg tgtacatgat cgcggcgggg tgccgcgacc cgtcgtccgg cgcgaacacg
     3121 tagaccgggc agtcgccgtc cgccgtcttg acggtgatct tctcttcttt catccgcttc
     3181 ccctcgtcgt tcgctcgtcg ggcacgaacg gtgcccgagt gccgggggcc cgggcgcccg
     3241 ccagggcggg agcggctttt ccggcacacc tcgatggacg atactcgtcg ccggctttgc
     3301 ggggcgtgag ggttcggcaa gagaaggcgg gccccggcgg cagaaggaag atcacgggct
     3361 gctgcccgta ctctggcatc gatgaccttc gatctgtctc cgtgccggcc tcggccggcc
     3421 gtgtcgtgat gccgcgtcgt cgcctgtgcg tggcgcaagg cgtgagcgcg ttcttcgcga
     3481 aggcctggct gttgttgctg ccggcgtggg cggggcgcaa cgtgaaggtg atcctggcgg
     3541 cccgcgtggg aatgagcgtg gggcgttcgg tctcgtcggt cgtgacggcg ctgtacctcg
     3601 cgaccatcgg gttcagcgga gtcgagatcg gcgcgttgtt cgtcgcggtc acggtcgcgt
     3661 cggccctgat gagtacctgc atagggctcc tggccgaccg gtgggggcgt aaaccgtttc
     3721 tcgtcgtcgt tccgctgatc gccgctgctg ccggcgtcat cttcgctgtg acgcgggtca
     3781 ccgccgtgct ctttctcgca gccgcggtcg ggtcgttcgg ccgcggcgcg ggggctggcg
     3841 gcggcagcgt gggaccttac cggccggcgg agtcggcgat cgtcgccgag acggtgccgg
     3901 cccgacaccg cgcggcagcg ttcgcccgca tcgcgtccgc ctcgtcgctc ggcgcgctcg
     3961 tcggcgggct caccgcccag ctcgtgcacg tgcatgcgca tatgagcccg gccgaggcga
     4021 ccgcggcgta tcggccggcg ttcttgatgg ccgcggcgct cgccgtcgtt gcgggccttc
     4081 tcgcgctcct cctgcgcgag ccggaacgtg cgccgcgtca atcaggccgc gcgccactgc
     4141 gctggccgcg ccgttcctgg ccggcgttgt ggcggttctg gatcacgaac gcgaccaatg
     4201 gggcgggcgc cggccttctg gggccgttcg tcagctactg gttttttcgc cgctacggcg
     4261 ccacgcccgc gcagatcggc gtgctgttcg ccgtcgtgaa cttcttttcc ctcgcttcca
     4321 ccctcgcctc cgcccgagtc gccgccaggc tcggcacggt gcgcgccatc gtcaccgtgc
     4381 gggcgatcgg cggcgttttg ctcgtgccca tggtgctcgc gccgtctttc ctgctggccg
     4441 gcggcatcta tgtggttcgc atgatggtcc agcgcgtcgg cctgccgttg cggcagtcgt
     4501 tcacccaaga catggccgac cccggggagc gggccagcgt cgccgcgctc tcgaatctgc
     4561 cggcacaagg cacgatggcc gccggtcagg ccgcgggcgg ctggctgttc gacgaggcca
     4621 gcctggccgc gccgttcacg ctcgccgcgg tgttccaggt cgccaacgcc gccctctatt
     4681 ggctgctgtt ctcccgccgg ccgcccaggc cgcgggcagc gggcgccgcc gacacccgct
     4741 cccggcgggc gtgacgcttc accgtgcgac ccgcacggcg tcgcggcgag ggcccggcgc
     4801 cccgtcacga ccctgcgccc tgccggcgac gacatcgggc ccgaggtcca ggcgccgcag
     4861 gagctgcgcg ttgacggcga ccacgagcgt ggacaccgac atgagaatcg ccccgaccga
     4921 catcggcaag acgaatccga ccggggcgag gacgccggcg gccagaggca cggagacgag
     4981 gttgtacccc gcggcccacc acaggttctg cgccatcttc cgatagccgg cgcgggagag
     5041 ctcgatcatc gacaccacag cgcgcgggtc gtcgccggcc aggatcacgc cggcggagcc
     5101 gacggcgacg tcggtgccgg cgccgatcgc gacgcccacg tcggcggcgg ctagagcggg
     5161 ggcgtcgttg acgccgtcgc ccaccatggc gacgcgccgt ccctctgccc gaagctgcgc
     5221 gaccttggcc gctttgtctt cggggcgcac gccggcgaag aagcgatcca ccccgagcgc
     5281 ctgggcggcg taggccgcca cgggatgcgc gtcaccggtg atcatgacga cgcggatgcc
     5341 gcgcgcgtgg agggcgtcga ccgctgcgga ggattcctcc cgcagttcgt cctccagccg
     5401 cagggctccc gccgggtcgc cgtcgaccag cacgtgcagc acggtggcgc cggcctcgtg
     5461 ccaagcccgg gcgtcgtccc gtacggcagg gtcgtcgccc acgggcagtc gcgcgcctcc
     5521 cacctgcacc agccggcccc ggaccagggc gctcacgccg acggccggcg atgacgagaa
     5581 gtcggaggcg gccggtacgt cgaggccgcg ggcgcgggcg gcagcgacga tggcgcgggc
     5641 cagcgggtgc tctgaatccg cctctgccgc ggccgcgagt gacaggagac cctcgtcgcc
     5701 gtaggccgga tccgcgctga ggtcggtcac gacgggccgt cccttcgtga gggtccccgt
     5761 tttgtcgaac aacaccgtgt ccacccgccg catcgtctcg agggcgagcc ggtctttcac
     5821 gagcaccccg gcgtgcgcgg cgcgctctgt cgcgatcgac acaaccaggg gaatcgccag
     5881 cccgagcgcg tgcgggcagg cgatgaccaa cacggtgatg gtgcgcatgg cggcgtcgtc
     5941 gggtgagccc aggctcgccc agacgacggc cgtcacgacg ccgacaccca gcgcgaacca
     6001 gaacagccag ccggcggcac ggtccgcgat ccgttgcgca cgcgacgacg actcctgcgc
     6061 ctcggcgacc agccgggcta tgccggccaa ggcggtgtct tccccgactg ccgacacgcg
     6121 aatccgcagg ccggagtcgg tggccacggt gcccgcgacg acggcatcgc ccgggccgcg
     6181 gcgaacggtg cgggactcgc cggtgatcat cgactcgtcc atgcttgccg cacccgagac
     6241 cacccggccg tctgccggca cccgggcacc ggggcgcacc acgacgaggt cgccgaccgt
     6301 gaggttcgcc gggctcacgg ccacggtggc ctcaccgtct acgcgccagg cgacgtcggg
     6361 caggagcgcc gccagagagt ccagcgctga ggtcgtctgg gcgagggagc gcatctccac
     6421 ccagtgcccc aagagcatga tgacgatcaa gagcgccagc tcccaccaga agtccatgtg
     6481 ggcgtcgacg aggcccagca ccgaccccca ggaggccagg aaggcgacgc tgatcgcgag
     6541 ggcgatcaag agcatcatgc cggggcgcct cgcgcgcatc tcgccgactg cgcccgagag
     6601 gaagggcagg ccgccccaca cgtacatcgc ggtgccgacg accggcgaca cccacgtggc
     6661 ccatgccccg cccggcagtg ggtaccccac gatcgaggcg aacatgcccg agaagcccac
     6721 cgtgggtgtg gccaagatca acatcaccca gaacagccgg cggaagcggg cggcgtgccc
     6781 gccgtgcccg ccgtgttccg catggtcggc gtgcccgctg cgttcaggcg gggcggtgtg
     6841 cgaggcatgc ccggcgccgc gtgccggggc cgacgcccca tgccgcggcg ccatgtcgtc
     6901 gtggtgggtg tgctcggcca tctgcgcctc ctgattccgt cggtaacaag aacctaatac
     6961 ccctgggggg tattccgctt gccttcggta tacctgtggc ggtgcccggg cggctcgtca
     7021 gagcgtggag gtgtcgatga cgaaccggta gcgcacgtcg gaggcgagca cgcggtcgta
     7081 ggcttcgttg atctggtcgg ccgagatgac ttcgatgtcg gcgccgagat ggtgttcggc
     7141 gcagaagttc agcatctctt gcgtctcgcg gatgccgccg atcgacgagc cggcgaaggc
     7201 tcgccgcatg tcgagcagcc tgccgacatc gacggagagc gggtcggccg gtgcgccgac
     7261 gttcaccaga gcgccgtcga gtgccagcag gtccaggtag ctgttgatgt cgatcgccgc
     7321 gctcaccgtg ttcacgatga ggtggaacgt gttcgccagg cgggtgaagg tcgcggggtc
     7381 gctcgtcgcg aagaaacggt ccgcacccag ccggcgggcg tccgcctcct ttccgctcgt
     7441 gtgggacagc accgtgacct cggcacccat ggcgtgggcg atcttcaccg ccatgtggcc
     7501 tagcccgccg aggccgacga ccgcgacgct cttgcccggg ccggccgccc aatggtggag
     7561 cggtgaatat gtcgtgatcc cggcgcaaag aagcggggcg gcctcgtcca ggccgacgcc
     7621 ggcggggatc ttcagcacga aatcctcggt cacgacgatc tgggtggagt agccgccctg
     7681 cgtgatgttg ccgtcgtggt cggccacgcc gtaggtgccg atgttgccgg ccaggcagta
     7741 ttgttcgttc cccgccagac agttgacgca ttcgccgcag gaatcgacca tgcagccgac
     7801 gccgacgcgg tcaccgaccg cgtgcttggt gacggccgta ccgaccgcgg cgacgatgcc
     7861 cgcgatctcg tggccgggcg tcaacgggta gacctgcgct ccccagtcgc cgcgcaccgt
     7921 gtggatgtct gaatggcaga tgcccacgaa cttgatgtcg atgagcacgt cgtgggggcc
     7981 gacctctcga cgctcgatcg tggtcggcac gagcgggctg ctcgcggcgg gcgcggcgta
     8041 ggccttgacg gtagacatga acctcctggt atggccgaag ggccggtagg tggatcggac
     8101 ggaggatctc gccggtaaag tggcggcatg cctcttcgcc atatcgtctc atggcgcctt
     8161 gccgccgggc cagtacgggc ccggctcgcg gccgagatcc gcgagcgcct tctcaccttg
     8221 cccagccagg tgcctgagat ccgctcctat gaggtcgggg tcgacgacga ggcggcggcc
     8281 gacggcttcg acgtggtcct cgttgcggag ttcgacgacc ggcaggctct ggcgcgctat
     8341 gcggcgcacc cggcccatca ggccgtcgcc gagttcattc ggtcggccgc agaggcccgc
     8401 agcgccgtca actaccgcgt ctgagccggc gccgccgtga gcctgagtcg agtggcggtc
     8461 ggctcctgta ggcatgccct gtaggtatct cgggctgtgt gccctggaat cccttgtgtt
     8521 tattggggtg agtaacgggg ctcgaacccg cgacctcctg gaccacaacc aggcgctcta
     8581 ccagctgagc tacacccacc atgtcgacgc gcgcgtgacg cccgccagtc tattacatcc
     8641 gcgacgccca ctcgtcggtc accgtggcgg cgaccgactt cacggtgtcg gtgtcggggc
     8701 cgggatcggc gacgaagacg accctgcggt agtagtccag ctcgcggatc gactctcgaa
     8761 tgtcggccag ggcccggtgg ccgccgtgct tctcgggggc gttgaagtac acccgtgggt
     8821 accaccgtct cgccagctcc ttgatgctcg acacgtcgac gttgcggtag tgcagcctgg
     8881 cgtcgaggtg gggcatgtat cgcacgagga acatgcggtc tgtaccgatg gtgttgccgg
     8941 ccagcggcgc ctgcttgtcg tggggcacga aacgatcgat gtagtcgatg acctgggttt
     9001 ccgcgtcggc gagcgctagc cccgtgggga tctcgtcgag gagcccgctc gtcgtatgca
     9061 tggcgcgcac gaagtcgtcc atgtgagcga gggccgcgtc gccgggtttg atgatgaggt
     9121 cgaggccggc gtcgagcacg gtcaacgcga agtcggtgac gatgacggcc acctctacca
     9181 actcgtcctt ctccgggtcg agcccggtca tctcgcagtc gatccaaacc aggcggtcgg
     9241 gggaagtgga catggccgaa gtctatcgag ccgcatgggg ccgactgtct tcgtaccggc
     9301 ggcggagaaa agggcggccg tagactcgct ctgtgcagtt cgagctatac acgtcggcct
     9361 tctgcggacc ctgccggggt gcccgccagg ttctcgcgga tgccgaacgc ctcgtcccga
     9421 gaatccacgt gcgggagttc gatgtggtgc gcgacgaggc tcgggcgaag gagcaagaca
     9481 tccgctcgac gccgacgatc atcgtccgcg acgggacggg ccgccaggtc tttcgcgccc
     9541 agggcgtgcc ccggctcggc caagtgttgg tcgccgccgc gtcggctctc taggtagacc
     9601 ccgtatcgac ggtgccgacc ggccccgcgc cgacgcccct gtcgccatcg ccgcctcgtc
     9661 tacgacgcag ggtgccccgg gcgggagtcg aacccgcaac ctacggatta gaaggccgtt
     9721 gctctatcca ttgagctacc agggcgaagg ccctccaaga atactcgaag cgcccgcgct
     9781 tcgggccgca ccgcaggggc atccgcgggc gtcacgcggg gcggcgtcgc cgtaggcgtc
     9841 gggtgtgtcg tctgccggca ggggacgatt gcgggtggga gaccgcaagg cggctttcac
     9901 ggcgcacgat gccgagccgc ggcaccgcgt ggtgcagtgt cagccgcacg cgcaggcggc
     9961 cgcgtcgggc gaggctcgcc gcgatgacgc ccgcacacac gaggggaacg gagcctgtca
    10021 gagccatcgc caggtgcggc ccgaacgtct ccaccgccca ccccgagacg gggccgccga
    10081 ctgcttggcc gcccagccag acgaggatgt agagcgccat gacccgcccg cgcacccgca
    10141 tgttcgacga catctgcacg agctggttgg cggcggtgat gaagagcagg ttcgcgaggc
    10201 cgaacgagac gagcaccgcg acgaacgacc actcgtcggg catgagcccg gcgacgagct
    10261 gcgtgcctcc gaccacggcg ccgcctgaca cggtgaggcg cagccgcacg gtcgctcgac
    10321 gggtcgaaag gagcgccccg gcaagtgcgc cgcacgcgac gagcgcgttg aacatgccgt
    10381 agccgccggc gccgacgtgg taaacgtcgt tcgcgaacga ggtgaggaac acgggcatcg
    10441 actgggcgaa aacggcgacg agcgccatca tgacgatcgt ccagagaata ggaggcttgc
    10501 gcagtacgta tgcgaggccc tccaccagct gacctttgcc gcgcggcaca accggcgccg
    10561 catagagttc gccggcgcgg atgcggaaca ggttcgtgac gacgagcgca cagccgacgg
    10621 cgttgacggt gaacatccag ccgccgccga tcaccgcgat caagaccccg gcgagggccg
    10681 ggccgacgag gccgcccagc tggaagatcg acgagttgat gctgatcgcg ttgcgcaggt
    10741 aggtcggccc gacgagctcg ttgacgaaca cctgccgggc tggattgtcg acgaccgtga
    10801 cgacgcccag aagcgccgag accgcccaga tgtcccagac ggtcacgatg ccggtcaacg
    10861 tgagaaccgc catgagcgct gccagaagcg cagcagcgct ctgggtgccg accaagaggt
    10921 tgcgtttcgg aaaacggtcg gcgacgatgc caccgaagag gccgagaaag atcatcggcg
    10981 tgaactgcat cgccatcgtg atcccgaccg cggtcgggct gcccgtcagc tgcagaacga
    11041 gccagtccat cgcgatgcgt tgcatccagt tgccggtgtt ctgcacgaga ttcgacgagg
    11101 cgaagagctg gtagttgcgc accttcagtg cgacgaacat ctcgctccac ggtggtcgtt
    11161 cggcgacgat cgggatcgcg gcggttatgg cagggtcgac cacgtgcggc ggagtcatcg
    11221 agaaagcagt ccaaagatgt taggtcggag gtctgggccc tgatggcgcc ccgacttttc
    11281 caggttaaac acggcccagt catgttggaa gccgatcgcg actataaata gcattgtgga
    11341 tcccaatgat gtgacggccg gcctgcaggc cgccgtgcgg cccttcgaca ccgtgctcct
    11401 gcgcaccttt ctcgcggtcg ccgacaccga gagcttcacg cgcgccgcgg aacagctcgg
    11461 catcagccag ccgacggtca gccaacatgt ccggcgtctg gaggaggccg cgggcagcgt
    11521 gctcgtcacc cgtgacacgc gccatgtgca cctcaccgag aagggcgagg cgatggcggg
    11581 cttcagccgg cgcatcctcg cgatccacgc cgaggcggtc agtttctttc gcggctcgca
    11641 gatgcgcggc aggctgcgct tcggcaccgc cgacgacctc gcgatcacgc agctgccgcg
    11701 catccttcgg cattttcgcc agctgcatcc gcagatcgag ctggagctga ccgtgagcca
    11761 gtccgccgca ctgttgcgcc gcctgaaagc cggccagctc gacctcgtct tcatcaagca
    11821 ggagcccgac gaggcgaccg gtatcccggt gcgtcgcgac gtgctcgtct ggatggggca
    11881 ggacaagacc ctcatcgacg agaacgacgc ggtgccgctc gtcacctacc agacgccgag
    11941 cctcagccgg cgcacggcga tccgtgccct ggagtcggtg ggccgacggt ggcgcatcac
    12001 ctgcaatatc aaggatgtca acagcacgct ggcggccgtg cgggcagggt tgggcatcgc
    12061 cgtctttccg cagtccctca tccccgacga cctcgtcaag gtgaccaggc gtttcgggct
    12121 gccggagctc ggcgacgtga tgttcagcct tctgaaaagc ccgacggcgc cggcggcgcc
    12181 gagcgcggcc ctcaccgagg cgatcatccg ccgcgacctg cgccgccgag ggcatgccgt
    12241 gcgggcttga tacgctgcca ccatgcgtgt gctgcagtcg gagatcatcc gcgcgctaca
    12301 tgtcgaaggc acgatcgatc cgcacgacga gatcttgagc cgcgtgcgct tcctcgccga
    12361 ctatgttggt cgcacggggg ccgcgggcct ggtgctcggc atctcgggcg ggcaagactc
    12421 ctcgttggcg ggccggctgt gccagctggc ggccgagcaa ttgcgcgacg ggggtcgaga
    12481 ggcacggttc gtagccgtgc gcctgccgta ccggcggcag cacgacgacg atgacgcgcg
    12541 tctcgcgctg gatttcatcc gtcccgatga gcaggtggag atcgacattg cggggccggt
    12601 cgactctctc gtcgccgctt gcgccgccgc gggccgcccc ctgagcgact ttgaccgtgg
    12661 caacgtgaag gccagggtgc gcatggcggt gcaatacgcg gtgggcggcg ccgacaagat
    12721 gctggtcgtc ggcaccgacc acgccgccga ggcggtcacc gggttcttca cgaagtacgg
    12781 cgacggtgcg gcagacctca ccccgctgac cggcctgacg aagcgacagg gcgccgcact
    12841 cctggcggca ctgggcgcac cggaacgcct ctggcagaag actccgaccg ccgacctgtt
    12901 ggatggccgg cccggccagc ccgacgaggc cgagctcggc atcacctacc aggcgatcga
    12961 cgactacctc gaaggccgcg aggtccagcc ggcggacgcc gaggcgatcg agcgccgcta
    13021 ccgttcgagt gcccacaagc ggcgcctgcc ggcgacgccc ttcgactgac gcacgccgac
    13081 ccgcgtggcg gccggcgtag ctcgggtggc cgccgcaggc cggcgacgag gctagtcggc
    13141 gccggggccg gtcgggagca tgcccgggtt ctggtcgacg acgagttccg acgcgggcgc
    13201 agtctcgccg tcggcctgtc tcgcggcctc gggcgccccg ggggtcgtcg gggtcgccac
    13261 cgtcttggtg agagcctcgt cgtcttcaag gtcttcggct cgcaggcctt ccgccgccgg
    13321 ccccgccggg tctggtcgag acgtgttcgc cgccggatcg ttctgtcggc gcgcggcctc
    13381 gcgatcctcc ttctcggctt ggtattcgaa cgaccaccgg tcgaggtctt cttggaacgt
    13441 cttgtggatg cgccgcgggt ggtattgcca ttcgatgagc ctgtcgcgca gctcggcgac
    13501 ggccgggtcg agctgatagc tgtacgtcgc cgagtggcgt ttcatctcgg cgagttgatg
    13561 cgccgcccag ttcgccgccg tgcccgagcc gcgaaccggc atgagtcgaa tgagcgtgtc
    13621 ggcctgtccg accgcccggt cgctcaggat ctgctcttgc ggcgtcagcg agttccacac
    13681 cgatgcctcc gtcgccgcgt cgacgatagc ggtgatggcc gacgcttttt gttggcgctc
    13741 cgtgcgggtg agaacggatt ggagggcacc gcgagagatc cacgcggcga ggaggccggc
    13801 cacgatgact gccagggcaa gcacgatcgc ggtgaaaacc accggctgcg tgtcgtcgga
    13861 ggtaagccag gccgtgaagt ctgagagcca ctgcatgcgg gcatcgtatc ggcgcgacgg
    13921 tcacgggcgg cacaacccgt cagcgcgtcg cccaaagagc aggccggtct gaggcctgcg
    13981 gacccggtca gtgcgagggg aggtcgacgg cgagcggttc gatctcggcc agctccgccg
    14041 ccgtcaggtc cgggccttcc agcgcggcga cgttctgctc cagttgggcg acgctcgagg
    14101 cgccgacgag ggcgctcgtg acttccggcc ggcgcagaac ccaccgcaac gcgagctgcg
    14161 cgaggctctg gccgcgccgg cgggcgatgt cgctcaaggc gcgcgcgcgg gcgaggtacg
    14221 cgtcgtcgat gcgagacggg tcgaggaagc ggctggtcgc ggcgcgcgag tctgccggga
    14281 tcgcgccgtc gaggtagcgg ttcgtcagaa ggccctgcgc gagcggcgag aacacgattg
    14341 ttccggcgcc gacctccgtg acggcatcca gcaggccgtg ttccacctgt cggtcgaaca
    14401 gggaatatct cggctggtgg atcgtcaggg gcaggcccat cgagcggagg atcgccgccg
    14461 cctcacgcgt ctcctccggg gagtagctcg agacgccgac gtacagcgcc ttgccgagcc
    14521 ggaccgcgtc ggccagcgct cccatcgtct cttcgagcgg cgtctccggg tcgggccggt
    14581 gatggtagaa gacgtcgaca tagtcgacgc ccaggcggga aagactctgg tccagcgacg
    14641 ccaggaggta cttgcggctg ccgtgatccc cgtacggccc cggccacatg aggtagccgg
    14701 ccttggtgga gagaatgagc tcgtcacggt ggtggcgaaa gtcgccggcc acgaggcgcc
    14761 ccagggtctc ttccgcggcg ccgggcggcg ggccgtagtt gttcgcggcg tcgatatgcg
    14821 tcacgccgag gtcgaaggcg cggcgcacga tggcgcgctg ggtctcgaac gatcgggagg
    14881 ggccgaagtt gtgccagatg ccgagggaga cggccggcag gagaaggccg ctcctcccac
    14941 tgcggcggta ggtcatggtg tcgtagcggt gggcatcggg agcgtaggtc atgcgttcat
    15001 ggtaggagaa tagaagaggt agaaaggaac ccgttcccat gacaaccttt catctggccg
    15061 gcggctgctt ctggtgcctc gacgcggttt atcgcacgct gcgcggcgtc accgacgtca
    15121 cctccggcta caccggcggc acggtcgcgc accccagcta tgagctggtc tgcgccggat
    15181 cgaccggcca cgccgagacc gcgcgcgtca ccttcgacga gacacagatt ccggcagaga
    15241 cggttctcga cgtcttcttc accatgcacg acccgaccca gctgaaccgg caggacggcg
    15301 acatcggaac ccagtatcgc tcggcgatgt tctacaccga cgacgggcag cgtgccctct
    15361 tcgaggcggc ccgcgaccgt gccgccggct ggtgggggag caccgtcacc gccatcgagc
    15421 cgctgacgac gttctatgag gccgagggct accaccagga cttctacgcg aagaaccccg
    15481 gccagggcta ttgcatggtg gtcaccaggc cgaaggtgtt gaagattcgc aagcgtttcg
    15541 cgcagtacgc ccgctgacgg tacgggcgcc tggcacgtgc ggtgccgcgg ccggccgtgt
    15601 gctcgtggtc ggcgccgtta cctgagttgc cactgcacac gcaggcaggg ggtccggccg
    15661 tcctttctcc gtaagaggta agagggagga caccgcctcc tgcacaggat cgccccggcg
    15721 cgatgtcttc cgtggcggtc ggccacgcga cgtgtcgtgg cgcgcggtcg gccaggctcg
    15781 tcttagcacc cggggcacgc ccgggggcag aagagggagg ccatcatgcg cacacctgac
    15841 atcctcgccg tgacgggcac ggtcggcacc gacccggagc gtcgggtcgg tgaatccgga
    15901 atcgacatga cgtcctttcg cctggccacc aaccacagca ggttcgacaa ggcgaccggg
    15961 caatgggtgg aacagcccgc caactggtac acggtcagtg cctaccgagg ccttgccagc
    16021 catgtcgtcg cctcggtgcg caagggcgag cgtgtcacgg tcgtgggaag gttgagaatc
    16081 gcccgctggc agtccggcga gcggtccggc acctccgtcg agatcgtggc cgagaccgtc
    16141 ggcctcgacc tcacgttcgc accggcgagc cgcctcgcgc cgtcagaagg ccccgtcgcc
    16201 cgggccgcgg cgtcctctgc ggcgccgcct gcgccgccgc agacgcccgc cttggccaca
    16261 ccgcgagagg gtttcctacc ggaaccggaa cccgtcttct gagcttctac cgcttcgcga
    16321 tgccggccgg gcacgccggg taggctggtg cccggttgac atacggcgaa gagcgacggt
    16381 taaggttcta tggaatatat ctactcgatg gtccgagccc gcaaggcagt cggcgacaag
    16441 gtcatcctcg acgacgtgac catggccttc ctgcccggcg cgaagatcgg cgtcgtcggc
    16501 ccgaacggcg ccggcaaatc caccatcctc aagatcatgg ccggcctcga gcggccgagc
    16561 aacggcgacg ccgagctttc ccccggctat tcggtgggca ttctcctgca ggagccggag
    16621 cttgatgatt ccgcgaccgt gctcgagaac gtgcagcagg gcgtcggcga gttgaagggc
    16681 aagatcgacc gcttcaacga gatctccgcg gcattggccg accccgacgc cgattacgac
    16741 accttgctcg ctgagatggg aggtctccaa gaggccatcg atgccgccgg cggttgggat
    16801 ctcgacaacc agctcgacca ggcgatggat gcgctgcgct gcccgccgcc cgacaccccg
    16861 gtctcggtgc tttccggcgg cgaacggcgt cgcgtggccc tctgtcggct gcttctgcag
    16921 aagccggatc tgttgcttct cgacgagccc accaaccact tggatgccga gagcgtgcag
    16981 tggctcgagg cgcatctggc gcagtaccac ggcgccgtgc tggccgtcac gcacgaccgg
    17041 tacttcctcg atcatgtggc cgaatggata tgcgaagtcg accgaggtcg gctctacccg
    17101 tacgagggca actactcgac ctacctcgag aagaagcacg agcgcctgca ggtgcagggt
    17161 aaaaaggatg cgaagctcgc taagcgcctc gctgacgagc tcgagtgggt gcgcagcggc
    17221 gccaagggcc ggcaggccaa gtcgaaggcc cgtctggccc gctacgaaga gatggcggct
    17281 gaagcggagc gaggccgccc gctcgacttc gaggagattc agattccggc cgggccgcgc
    17341 atgggcagtc tggtcatcga ggcgaaagac ctcgtcaagg ggttcgacgg gcgcaccctc
    17401 atcgacgggc tgagtttttc gctgccgcgc aacggtatcg tcggcgtgat cgggccaaac
    17461 ggcgtcggta agacgacctt gttcaagacg attgtgggcc tggagtctct cgacgccggc
    17521 gagctcgtca tcggtgactc ggtgcagatc tcctacgtcg accaattgcg tgcaggcatc
    17581 gaccccgaca agacgctgtg ggaggtggtc tctggcgggc tcgactacat ccgtgtcggc
    17641 aagaccgaga tgccgagccg cgcctacgtg tcgacgttcg gcttcaaggg tcccgaccag
    17701 cagaagcgtg cgggggttct ttctggcggg gagcggagcc gcctgaacct cgccctcacc
    17761 ctcacccagg gcggcaacct gctgcttctc gacgagccga cgaacgacct cgacgtggag
    17821 acgctctcca gcctcgagaa cgcgctgctc gaattcccgg gctgtgccgt cgtcgtcacc
    17881 cacgatcgat ggttcctcga ccgggtcgcc acccatatcc tggcctacga gggcaccgag
    17941 gagaacccgg ccgcgtggta ctggttcgag gggaatttcg aggcctacga ggccaacaag
    18001 atcgaacgcc tcggccccga cgccgcccga ccgcatcgct cggtgtatcg caggctcacc
    18061 cgcggatgag gcgcctccac gtgccggtcc ggctgcggtg gagcgacgtc gacgcgtacg
    18121 ggcacgttaa caacgccgag atgttccggc tcctcgaaga ggcgcgcatc caggcgttct
    18181 gggcgcccga tctggctccc ggcctgcgtg aacccgccga gccgggcgag ggggcgtcgg
    18241 agacgacccg ggttctcgcc ggcggcgccg atgccgacac gttgtcgttc atcgcgaaac
    18301 acacgatcga atacctgacg ccgatcccct acatgagcga accgatcgac gtgcagctct
    18361 ggctcggccg gctcgggggg gcgagtctcg acgtctgcta cgaggcgcat ccgccgcgtg
    18421 gcctgccgga accggtctac gtgcgagcct cgacgactat cgtgctggtc gacgccgcca
    18481 cccgccggcc tcgccggctc acgcaacggg agcgggccgc gtgggggccg taccttggcg
    18541 accccgtcac gttcaacagg cactagctgg ccggggcacg gtgtcttcag cgccctccga
    18601 cccggatcat cccttcctgg gccacgctcg ccagcaatat gccgtctcgc gtatagatac
    18661 gacccgtcgc gaacccccgg cctcccgtcg cgctcggcga ctcttgcgcg taaagcagcc
    18721 aacgatccac gcggccgaac ctatgccacc acatggcgtg gtcgaggctc gcgatcttgg
    18781 cgtctcggtc tgaccagctg acgccgtgcc ggcgaaagac cgattccaag atcgaatagt
    18841 cgctcgcgta gacgagggcg gcacggtgca ggttctcgtc gtcgggcaag ggcgagcgcg
    18901 tcttgaacca gacgcattgg tgtgcgacgc gctcgccgcg caccttcagg tacacggggg
    18961 aggagacgtg ccgcatctcg aatgcccggc cctgggccca gtgccgggtg accgggtccg
    19021 tcagccgcgc gaggctcgcg tcgtcgccgg agagctcctc gggccggggc aggcccgccg
    19081 gcatgtcgag ctggtggtcg aagccctcgt cttgcgtctg gaacgaggtg atcatcgaca
    19141 agatcggcac gcccctctgg taggcctgca cccgccttgc cgagaacgag cggccgtcgt
    19201 gtatgcgatc gaccgcgaac gagatcggaa gggccgtgtc accggggcgc agaaagtagc
    19261 cgtgcaggga atggacgacc cgttccggcg gcacggtatg gcaggcggcg atcatcgatt
    19321 gggcgagtac ctggccgccg aacacccggc cggccggcat ccacaccgat cggccggtga
    19381 agatgtcttc gtgggtccgg gccccggcgt cggtcaaggt caagacgtcg agcagcgcct
    19441 cgaccggggt gcgtggtgtg gcctcggcgg cgcccgcccc ggtgcctggc ctcgccgtgc
    19501 gccccgcacc ccccgtggtc tcgccgctcg tctccgtgcc ggccccggtc acgagacccc
    19561 tgccgtgtgc atggcttcag tttaaaggcg accgcggcgc tgcccgcggt cgtgaactca
    19621 tcggccgggc tcgtccggcg gcctgttccg ccggcgccgt gtgtgccgct cggcgacgac
    19681 gttcagcggt ttggtgcgcg gcgggtgcag gccatagacg ctcatcgcgc cgtcgaaacc
    19741 gctggcgacc acggtcgcga gcgtcgggat gtcgacgccg agggcgtcca tcgtctcttt
    19801 cacgcgcgcc cgcagctcac gcgacacgtc gtctttcgcg cctgcgcggg tgcgcagcac
    19861 gatgcggatg acgagcgcgt tgtcggcgat cgactccagc ccccagagct ccgcctggcg
    19921 gaggatccgg gcgcgccatt tcggggtcgt cgcaagtttg ttggcttcgg ccatgagcgc
    19981 cgacttcacc tcgtcgaggt cggcgctcaa gggcaccgag aggtcgagga tcacccgcga
    20041 ccagccatgc gacatgttcc cgacccgcag aatctcaccg ttgcgcacga accagagcgt
    20101 gccgttcacg tcg
//
LOCUS       DASTNL010000191         8391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1621077, whole genome shotgun sequence.
ACCESSION   DASTNL010000191 DASTNL010000000
VERSION     DASTNL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621077"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFQ96_05970"
     CDS             complement(<1..321)
                     /locus_tag="VFQ96_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0257369.1"
                     /translation="MPRQIRFNAFDMNCVAHQSSGLWRHPDDRSRHYNDLEYWTGLAK
                     LLEAGTFDGIFIADVLGTYDVYGGNNVAAIRNGAQVPVNDPVLLISAMAAVTKNLGFG
                     VTAGT"
     gene            677..1948
                     /locus_tag="VFQ96_05975"
     CDS             677..1948
                     /locus_tag="VFQ96_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU0257370.1"
                     /translation="MRPPAPATRLTIIADDLTGAADAAAALVPHGECSVLLTFDSQRL
                     PSAITAINTVSRDVAAHVAYERVAKASHLALGAGSTLYKKVDSLLRGNVGTELDAVLA
                     TARRHDGLGRARLVVAPAFPAMGRTTVGGRVEVRGRPRPDIPYGGDVGAALQIAGLAV
                     AVVGRPGGETRLRQALESFATTSVDAIVVDATSDDDLRLIARASECLSYPAILAGSGG
                     LSRHLAQHGPAPTHQAFASAAGGRPLPGPVLFVCGSHSLEALAQAKRLVEAGLHEIAV
                     GVAPAGPHADPTPPSRNRAGAPAAIGAFVKDTLLRVDPRLEVHARHRAGVERALTDAA
                     AGLAPLYRTLVLTGGSTAQSLLVRLGVSAMRVEEEVLPGVVRSRADVVGLPVVITKAG
                     AFGDDDTLLDLARLSTGAEPSAPPSRRKDTP"
     gene            1945..2967
                     /gene="pdxA"
                     /locus_tag="VFQ96_05980"
     CDS             1945..2967
                     /gene="pdxA"
                     /locus_tag="VFQ96_05980"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0257371.1"
                     /translation="MKENRPVIGITMGDAAGVGPEIIVKALASDGFLGEAAPLVIGDA
                     RRLALAAKACRLDLAIRQVSSVGEAQFSARRVNVVDIACIPEDLPWGRVSAQAGDGAF
                     QFIERAVYLAMSGDIDAICTAPLNKEALHKAGHDFPGHTELLAHLSHTDEVSMMLTAP
                     GLRVIHVTTHLGLVDAVHSITPERVYRTIRRGHDLLTAAGADQPRIAVCGVNPHAGEH
                     GLFGNREEERQIAPGVQKARDEGIDAVGPLPADTVFGKARQGGFDLVVAQYHDQGHIP
                     VKLLGLQNGVNVTVGLPFVRTSVDHGTAFDIAGRGIADERPLLAAYREAVALAASRTQ
                     QIASTS"
     gene            2964..3734
                     /locus_tag="VFQ96_05985"
     CDS             2964..3734
                     /locus_tag="VFQ96_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEU0257372.1"
                     /translation="MTRDSRLDKIRRSLLLGPQSVESLASGLGISPSTVRRDLALLEK
                     AGGVIRTHGGAVIRPEGESSLAERESVAAAAKLAIGRLAAELVAEGSRVLLGAGTTVE
                     AVARMLATTKDLTVFTDSLPVMGILGPRPNIDLYAVGGRFRHLSASFFGPAAEKFVSA
                     ITVDHVFVSGDAIDAQRGVSERSAEQASLKAAMMRAGGSVYVVADGSKAGAPSPAGWW
                     TAPPTGWTLVTDTSATDAALEPFRHAGPTQILIAGRRA"
     gene            complement(3840..4358)
                     /locus_tag="VFQ96_05990"
     CDS             complement(3840..4358)
                     /locus_tag="VFQ96_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0257373.1"
                     /translation="MRVALASGGYDAVTIKGLAAGAGVSKQTIYRWWPTKAAILGEAL
                     LEGELPVPALPTTEDLGVDLRAWFMAMSAQARSGASVEVARALIAVTATDPELGAVLN
                     ARLAGPIVTQIVARLRAAQQAGDVRRDVDVAAIADQFIAMSSYAMLVGRLFDEERVDA
                     VVGVLLRGMLAH"
     gene            4491..5495
                     /locus_tag="VFQ96_05995"
     CDS             4491..5495
                     /locus_tag="VFQ96_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257374.1"
                     /translation="MALTAHSTIGDWLNDPAGGELIKDLLAQAGANTESLTPVLGLPL
                     QQLVALSQGQMPQSVIDGLVRSANGGEIPEDDPSDAGWTEQAITGRFSGRTVIVTGAA
                     SGIGKATASRVAREGGRVVAADIAAGKLEALAAELPNADIVVVAGDLTRQDAIDAVIA
                     AAGDHIDGLANIAGINDDFSPAGETLDAIWDRVIAINLTAPFKLMRAVLPLMERAGHG
                     AILNVSSEAGLRGNAAGNAYTASKHGVIGLTKSAAFMYGPKGIRVNSVAPGGVATGIP
                     MPPQVSEAGSTRLAPFQQAIPTLATAEHLAASITFLLSDDAVNINGAILPSDGGWSVQ
                     "
     gene            complement(5728..5799)
                     /locus_tag="VFQ96_06000"
     tRNA            complement(5728..5799)
                     /locus_tag="VFQ96_06000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5764..5766),aa:Gln,seq:ctg)
     gene            complement(6021..7547)
                     /gene="gltX"
                     /locus_tag="VFQ96_06005"
     CDS             complement(6021..7547)
                     /gene="gltX"
                     /locus_tag="VFQ96_06005"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU0257375.1"
                     /translation="MPSSRSYPVTTAASHDIRLRFCPSPTGTPHVGMVRTALFNWAYA
                     RHTGGKLVFRIEDTDAERDSEESYRQIVEALRWLQIDWDEGVEVGGAHGPYRQSQRGE
                     IYRDVIRRLAESGHVYESFATAAEIEARNVSLGRDAKTGYDNFERELTDAQRRALRDE
                     GRRPALRLRVPDTDLGFDDLVRGTITFPAGSFTDFVVVRPNGAPLYTLVNPIDDAMMG
                     VTHVLRGDDILSSTPRQIALYHALIDLGIASAVPRFGHLPTVLGEGTKKLSKRDPESN
                     LFHHRDRGFIPEGLLNYLALLGWGFSADRDVFTREEMIAAFDVADVKPNPARFDLRKA
                     ESLNGDHIRMLSDEDFAERSVPYLVRAGVLHEPVGAGDRAVLAAAAPLVKSRVRLLGE
                     TPGMFGFLFVDAAALVIDDDARATLKPDAGDVLVATRAALDEVGDAEWQHERIEAVLR
                     AALVESRGLKPRLAFGPLRVAVTGRRVSPPLFESMEILGRTESLARVDKLAVRLTQLA
                     "
     gene            complement(7628..8371)
                     /locus_tag="VFQ96_06010"
     CDS             complement(7628..8371)
                     /locus_tag="VFQ96_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0257376.1"
                     /translation="MAFGILDEEEREFVVLLGDPLYKGFDTTGERVKLAEARLLAPVI
                     PRSKVVAVGKNYREHAAEMGGVLPTEPLLFLKPNTAVIGPGDAIVLPPQSHRVDHEGE
                     LAVVVGAVAKNVRAEDAASVIFGYTVANDVTARDLQRSDLQWARAKGFDTFCPLGPVI
                     STELDVARTTVETRVNGELRQSGQTADLIHTIPKIVEYASSVWTLLPGDVILTGTPAG
                     VGPLTDGDTVEVSISGIGTLSNPVRGPQA"
BASE COUNT         1387 a         3016 c         2768 g         1220 t
ORIGIN      
        1 cgtgcccgcg gtcacgccga agccgaggtt cttggtgacg gccgccatcg ccgagatcaa
       61 gagcaccgga tcgttcacgg gcacctgcgc cccgttgcgg atcgccgcca cgttgttgcc
      121 gccgtacacg tcgtaggtgc ccaacacgtc ggcgatgaag atgccgtcga acgtgccggc
      181 ctcgagaagc ttggcgagac cggtccaata ttcgaggtcg ttgtagtgcc gcgagcggtc
      241 atcgggatgc cgccacaggc cggaagactg gtgcgcgaca cagttcatat caaaggcgtt
      301 gaagcgtatc tgtcgaggca ttcccccatc gtgctgtctg gggcgacagc acgcatctcg
      361 ggcgacacac gacgcaacat accggcgcga tccgcgggcc ccagacctgc cgacgcctgc
      421 cgacacccag cccggtcttc gacaccaccg acgtcgcctc cggctcaccg agacgatatg
      481 ctcatttcga gcacgtattt gattaaagca cgcaagacag tgtggcgcag ggccgaggcc
      541 gggtaggcga ggccgtgcca cacactcggc taggcaaggc ggtgacgatc gtgacgaaag
      601 agacccccca cccgcgcaac tccgctccgg cggtcgcggc cggtgtcgtg ccggccgggg
      661 cgcggacgaa ggcccgatga gaccgcctgc cccggcgacg cggttgacga tcatcgccga
      721 cgacctgacg ggcgcggccg acgcggcggc cgcgctggtg ccgcacggcg aatgctccgt
      781 gctcctcacc ttcgattccc agcgcctgcc ctccgcgata accgccatca acaccgtgtc
      841 gagagacgtg gcggcgcatg tcgcctacga gagggtggcc aaggcgtcgc atctcgcact
      901 cggggccggt tccaccctct acaagaaggt cgactccctg ttgcgcggca acgtcggcac
      961 cgagctcgac gccgtgctcg cgacggcgcg ccgtcacgac ggcctcgggc gtgcccgtct
     1021 ggtcgtagcg ccggcttttc cggccatggg gaggacgacc gtgggcggga gggtcgaggt
     1081 tcgcggccgg ccgcggcccg acatcccgta tggcggagac gtcggcgcgg cgctgcagat
     1141 agcaggcctc gcggtcgccg tcgtcggccg cccgggcggc gagacacggc tgcggcaggc
     1201 tttggaatcc ttcgcgacga cgagcgtgga cgcgatcgtg gtcgacgcga cgagcgacga
     1261 cgacctccgg ctcatcgccc gcgcctcaga gtgcctcagc tacccggcta tcctggccgg
     1321 atccggcggg ctttcccgac atctcgcgca gcacggcccc gccccgacac accaagcttt
     1381 cgcgtctgcg gcaggcggcc ggcccctccc tggcccggta ctcttcgtct gcggaagcca
     1441 ctccctcgaa gccctggccc aggcgaaacg gctcgtcgag gcgggcctgc acgagatcgc
     1501 cgtcggcgtg gcaccggccg ggcctcacgc agacccgaca cctccgtcga gaaacagagc
     1561 aggagccccg gcggcgatcg gtgcattcgt gaaagacacc ctgctgcgcg tcgacccccg
     1621 gctggaggtg cacgcccggc atcgagccgg cgtcgaacgg gcgctcacgg atgcggcggc
     1681 agggctcgcg cccctctacc gcacgctcgt cctcaccgga ggcagcacgg cacagtcgct
     1741 cctggtgcgg ctcggggtct cggccatgcg ggtggaggag gaggtgctcc ccggggtcgt
     1801 ccgcagccgc gccgacgtcg tcgggctgcc cgtcgtgatc acgaaggcgg gcgccttcgg
     1861 cgacgacgac accctcctcg acctggcgcg actctccacc ggtgccgagc cctcggcgcc
     1921 cccgtcacga cgaaaggaca ccccgtgaag gaaaaccggc ccgtcatcgg catcaccatg
     1981 ggcgacgcgg caggcgtcgg cccggagatc atcgtcaagg cgctcgcctc cgacggcttc
     2041 ctcggcgagg cggcacctct cgtcatcggc gacgcccgca ggctggcgct cgcggcgaaa
     2101 gcctgccgtc tcgacctcgc gatccggcag gtctcgagtg tcggagaggc acagttctcg
     2161 gcgaggcgtg tcaacgtggt cgacattgcc tgcatccctg aagacttgcc gtggggccgc
     2221 gtctccgcgc aggccggcga cggcgcgttc cagttcatcg aacgggccgt ctacctcgcg
     2281 atgagcggcg atatcgacgc catctgcact gccccgctga acaaagaggc gctgcacaag
     2341 gccggccacg actttcccgg ccacacggaa ctgctcgcgc acctctcgca caccgacgag
     2401 gtctcgatga tgttgaccgc cccgggcctg cgcgtcattc acgtgaccac ccacctcggc
     2461 ctcgtcgacg cggtgcactc gatcaccccg gaacgcgtct accgcacgat ccgccgcggg
     2521 cacgacctcc tgacggcagc cggcgccgac cagccgcgaa tcgcggtgtg cggcgtgaac
     2581 ccccacgccg gggagcacgg cctcttcggc aaccgtgaag aagaacgcca gatcgcgccg
     2641 ggcgtgcaga aagcgcgcga cgaaggtatc gacgccgtcg gtcctctgcc tgccgacacc
     2701 gtcttcggca aggcacggca gggcggattc gacctcgtcg tcgcccaata ccacgaccag
     2761 ggccacatcc ccgtgaagct gctcggcctg cagaacggcg tcaacgtgac ggtcgggctg
     2821 ccgttcgtgc gcacctccgt cgaccacggc acggcattcg acatcgcggg ccgagggatc
     2881 gccgacgaac gcccgctcct cgcggcgtac cgcgaggcgg tcgcgctcgc cgcgagccgc
     2941 acgcaacaga ttgcttccac gtcgtgaccc gcgacagccg gcttgacaag atccggcgca
     3001 gccttctcct cgggccgcag agcgtcgaga gcctcgcgag cgggctcggc atctcgccct
     3061 ccaccgtgcg ccgcgacctg gccctgctcg aaaaggccgg aggcgtgatc cgcacgcacg
     3121 gcggcgcggt catacgcccc gagggcgagt cgagcctcgc ggaacgcgaa agcgtcgccg
     3181 ccgccgcgaa gctcgcgatc gggcgtctcg ccgcggagct cgtggccgag ggcagccgcg
     3241 tactcctcgg cgccggaacg accgtagagg cggtcgcgcg catgctggcg acgacgaagg
     3301 acctcaccgt cttcaccgac agcctgccgg tcatgggcat tctcggacca cgcccgaaca
     3361 tcgacctgta cgccgtgggc gggcggttcc gtcacctgag cgcgagcttc ttcgggccgg
     3421 ccgcggagaa gttcgtctcg gcgatcacgg tcgatcacgt cttcgtgagc ggcgacgcga
     3481 tcgacgcgca gcggggcgtc tctgagcgtt cggccgagca agcctcgctc aaggcggcca
     3541 tgatgagggc cggcggctcc gtctacgtcg tcgccgacgg cagcaaggcc ggcgccccgt
     3601 ctcccgccgg atggtggacg gcaccgccca caggatggac gctcgtcacc gacacgtcag
     3661 caacggatgc ggcgctcgaa ccattccgac atgccggccc gacgcagatc ctcatcgccg
     3721 gccgacgggc gtgaccccgc acacgacatg ggcggggaag cagtacggaa caaagacttc
     3781 gggctcctcc gtgaccgagc gcccggcgct cgaggttaac gcctccgggc cccgggccct
     3841 cagtgagcca gcatcccccg tagcagcact cctaccaccg catccacccg ttcctcatca
     3901 aacagtctgc ccacaagcat cgcataggac gacatggcga tgaactgatc ggcgatggcc
     3961 gcgacatcca cgtctcgtcg cacgtctcct gcttgctgag cggccctcaa ccgagcgacg
     4021 atctgtgtga cgatcgggcc ggcgagcctg gcgttgagaa cggcaccgag ctcggggtcg
     4081 gtcgccgtca ccgcgatcag cgcccgtgcg acctcgacgc tcgccccgct tctggcctgc
     4141 gccgacatcg ccatgaacca ggcacgcagg tcgacaccaa ggtcttctgt cgtaggcagg
     4201 gcgggcacag gcagttcgcc ctccaggagt gcctcgccga ggatcgcggc cttcgtcggc
     4261 caccagcggt agatcgtctg tttcgacacc cccgctccgg ccgcaaggcc cttgatcgtc
     4321 acggcgtcgt agccgcccga ggccagagcg acgcgcatcg cctccaacac agagcggcgg
     4381 gctttctcac tgcgagggcg aggcacatgt cgagcgtatc gaggcgtacg ctgagctgtt
     4441 acgagacgca ccgttgcgta attgtggacg acaggggacg aggtaagacc atggcactga
     4501 ccgcgcactc caccatcggc gactggttga acgaccccgc cggcggtgaa cttatcaagg
     4561 acctcctcgc tcaagccggt gcgaataccg aatctctcac acccgtgctc gggttgcccc
     4621 tgcaacagct ggtcgccctc agtcaagggc agatgccaca atccgttatc gatggtctcg
     4681 tccgctcagc gaacggcggc gagatccccg aagacgaccc ctcagacgcg ggctggaccg
     4741 agcaggccat cacgggccgt ttttcgggta ggaccgtgat cgtcacggga gccgcctccg
     4801 ggatcggtaa ggccacagca tcccgcgtcg cccgcgaagg cggccgcgtc gttgccgccg
     4861 acatcgcggc cggcaagctc gaggcactcg ccgcagaact cccgaacgcc gacatcgtcg
     4921 tcgtcgccgg cgacctcact cgacaagacg ccatcgacgc cgtcatcgcc gctgccggcg
     4981 atcatatcga cgggctcgca aacatcgcgg gcatcaatga cgacttctcc ccagccggag
     5041 agaccttgga cgctatctgg gaccgcgtca tcgcgatcaa cctcaccgcc cctttcaaac
     5101 tcatgcgtgc ggtccttccc ctcatggaga gagccggcca cggcgcgatt ctcaacgtct
     5161 ccagcgaagc aggcctgcgc ggcaacgccg cgggaaacgc ctacaccgcc agcaagcatg
     5221 gcgtcatcgg cctgacgaag tcggccgcct tcatgtacgg cccgaaagga atccgcgtga
     5281 actctgtcgc cccaggcggg gtggccaccg gcattcccat gccacctcag gtgtccgaag
     5341 ccggttcgac acgcctcgca cccttccaac aagccatccc gaccctggca accgcggaac
     5401 acctcgccgc ctccatcacc ttcctcttga gcgacgacgc cgtcaacatc aacggtgcga
     5461 tcctccccag cgacggaggc tggtcggtgc agtagcattc cgcgcgggca gcgccggtcc
     5521 tcgtctcaca cggcgtggac cctgccgcgg cccccgcggt ccccggccgc gacgacacgc
     5581 gcccacattc atggcgtcga cgggcgggcg aaatcgccgg tcgcgcttct gggtaaggtg
     5641 cacagcgagg tgcacgccga gaacacggtc gatgagaaag agtcggtgaa aaggcgaaac
     5701 ccccggtgaa ccgagggtct ttcagagctg gggtgcctgg actcgaacca agaacaactg
     5761 gaccagaacc agccgtgttg ccaattacac cacaccccac cgcccgtggc agccctcagc
     5821 tgccggccgg cgtgacgcgt caccgcgccg ggttcaagga tacgtggcgg ggcacccttt
     5881 ggccaacccg gcccgccgca cggagccgaa gcgtaggccg cctccggcct tgatcctcca
     5941 ccggccgggc agccccaaac ccgccgggcg acgagcggcc cgccgggcga cgagtgccgc
     6001 cgaccgcggg cagcaccgcc tcacgccagc tgcgtgagcc gcaccgcgag cttgtcgacc
     6061 cgcgcgaggc tttccgtgcg gccgaggatc tccatcgact cgaagagcgg cggggaaacg
     6121 cggcggcccg tgaccgccac ccgcagcggc ccgaacgcga gacgcggctt caggccacgg
     6181 ctctcgacca gcgccgcccg cagcaccgcc tcgatccgct catgttgcca ctcggcgtcg
     6241 cccacctcgt ccagcgccgc ccgggtcgcg acgagcacgt cgccggcatc cggtttgagc
     6301 gtcgcacgcg cgtcgtcgtc gatgacgagg gcggccgcgt cgacgaagag gaacccgaac
     6361 atgcccgggg tctcccccaa cagccgcacg cggctcttga cgagtggcgc ggcggcggcc
     6421 agcacggcgc gatcccctgc accgacgggt tcgtgcagca cgccggcccg cacgagataa
     6481 gggacgctgc gctcggcgaa atcctcgtcg gagagcatac ggatgtggtc gccgttcagc
     6541 gactccgcct tgcgcaggtc gaagcgcgcc gggttcggct tcacgtcggc cacgtcgaac
     6601 gcggcgatca tctcctcccg ggtgaagacg tcacggtcgg ccgagaagcc ccagccgagg
     6661 agcgcgagat agttgagaag cccctccggg atgaacccgc gatcccggtg gtggaacagg
     6721 tttgactcgg ggtcgcgctt cgacaacttc ttcgtaccct cccccaacac cgtcggcagg
     6781 tggccgaacc gcggcactgc tgacgcgatg ccgagatcga tcaaggcgtg gtaaagcgcg
     6841 atctgccgcg gggtggagga aaggatgtcg tcgccgcgca gcacgtgcgt cacacccatc
     6901 atggcgtcgt cgatcgggtt cacgagggtg tagagcggcg cgccgttcgg cctgacgacg
     6961 acgaagtcgg tgaaggagcc ggcagggaac gtgatcgtgc cgcgcacaag atcgtcgaag
     7021 ccgaggtctg tgtcgggcac gcgcaggcgc agggccggcc ggcggccctc gtcgcgcaac
     7081 gcgcgccgtt gcgcgtcggt cagctcccgc tcgaagttgt cgtacccggt cttcgcgtcc
     7141 cgcccgaggg agacgttgcg cgcctcgatc tcggcggccg tggcaaagct ttcatagacg
     7201 tggcccgatt cggcgagccg gcggatgacg tctcggtaga tctcgccgcg ctgcgactgc
     7261 cggtacgggc cgtgcgcacc gccgacctcg acgccctcgt cccagtcgat ctgcagccac
     7321 cgcagtgcct cgacgatctg ccggtagctc tcttcgctgt cgcgttcggc gtcggtgtct
     7381 tcaatgcgga agacgagctt gccgcccgtg tgccgcgcgt acgcccaatt gaagagcgcc
     7441 gtgcgcacca tgccgacgtg cggcgtgccg gtcggcgatg ggcagaaacg aaggcggata
     7501 tcgtggctgg ccgccgtggt gaccgggtag gagcgcgaag aaggcatagg gcctcagttt
     7561 atcggccgcc cgctgcgccg gatcccggcg tgaaccaagc cgccgccggg cggccgcaca
     7621 ccccgcttca ggcctgtggg ccgcgcacgg gattgctgag cgtgccgatg ccagagatcg
     7681 agacctccac cgtgtcgccg tcggtgagcg gcccgacgcc ggcaggagtg ccggtcaaga
     7741 tcacgtcgcc cggcagcagg gtccagacgg aggaggcgta ttcgacgatc ttggggatcg
     7801 tgtggatgag gtcggcggtc tggcccgact ggcgcagttc gccgttcacg cgggtctcga
     7861 ccgtcgtcct ggcgacgtct agttcggtgg agatcaccgg cccgagcggg cagaacgtgt
     7921 cgaagccctt ggccctggcc cattgcaggt cgcttcgctg caggtcgcgg gcggtgacgt
     7981 cgttggccac cgtatagccg aagatcacgc tcgcggcatc ttcggcccgc acgttcttcg
     8041 ccaccgcgcc gacaacgacg gcaagctcgc cctcgtggtc cacccggtgc gattgcggag
     8101 gcaagacgat ggcgtcgccc gggccgatca ccgccgtgtt cggcttcaag aagagcagcg
     8161 gctcggtggg cagcacaccg cccatctcgg cggcgtgctc gcggtagttc ttgccgacgg
     8221 cgaccacctt ggagcgcggg atgaccgggg cgagaaggcg tgcctcggcc agcttcaccc
     8281 gctcgccggt cgtgtcgaag cccttataca acgggtcccc taagagcacg acgaactcgc
     8341 gctcttcctc gtcgaggatg ccgaaggcca aggtgtcgcc gtgcttgaac c
//
LOCUS       DASTNL010000192         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_697435, whole genome shotgun sequence.
ACCESSION   DASTNL010000192 DASTNL010000000
VERSION     DASTNL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697435"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFQ96_06015"
     CDS             complement(<1..825)
                     /locus_tag="VFQ96_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0257377.1"
                     /translation="MSTATESRLVGHVIGGSPVESGDEAIERRAPGTDEVVSRVLAGG
                     AREVEAAARAARHAFDEGPWPKMTGMERSILVGKVAELIERDSARLAVLDAEETGKPI
                     KLAEGDIAGAAGLWRYAASLALTMHGSSMTNNGPDFTGLVFREPVGVVGLIVPWNFPA
                     LILSQKLPFAIAAGCTAVVKPSELTSSSAREILRLVAEAGIPEGVVNLVVGDGRAGQA
                     ISESMGIDLVSFTGSTVTGRRVIAASAGNIKKLSLELGGNAASIVFADADLEDAAAG"
     gene            complement(839..1975)
                     /locus_tag="VFQ96_06020"
     CDS             complement(839..1975)
                     /locus_tag="VFQ96_06020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU0257378.1"
                     /translation="MSRHFSSPPRLVMGEGALEEVAAELPRLGTRGLIVTDPGIMAAG
                     IHGLVRGILGRDFPVETFSGVVGNPDIATVEAADEARRAAGADVLIAIGGGSSIDVAK
                     SAALLGTNGGGIADYEGVDRFDKRPLPVVVVPTTVGSGSEVTKGAVITNPDTHVKMVV
                     VSDLMFATIAVLDARVVEGLPGRIAATTGMDALTHAIEAYVAKGANPVTDALCLGAIE
                     IIGKNLVPASRGDKTALYNMLVASSMAGIGFHDAGLGAVHALANTLGAQFGVHHGTAN
                     ALFLPYVIDFNIPAAPERFARIAAALGEEVAGLAPAKAALRASAAVHRLAEETGVPRH
                     LSDVGVGADAIPALARDALTQADLPGNPREVTLADLTDLYTKAL"
BASE COUNT          314 a          709 c          734 g          319 t
ORIGIN      
        1 gccggcggcg gcgtcttcga ggtcggcgtc ggcgaacacg atgctcgccg cattgccgcc
       61 caactccaac gaaagcttct tgatgttgcc ggcggaggca gcgatcacgc ggcggccggt
      121 cacggtcgac ccggtgaagg agaccaagtc gatgcccatc gactccgaga tcgcctgccc
      181 ggcacgcccg tcgccgacga cgaggttcac gaccccctcg gggatgccgg cctcggcgac
      241 gagccgtaga atctcccggg cgctggaact cgtcaactcc gacggcttga cgaccgccgt
      301 gcagccggcg gcgatcgcga acggcagctt ctggctgagg atcagcgcgg gaaagttcca
      361 cggcacgatg agcccgacga cgccgaccgg ctcccggaag acgagtccgg tgaagtcggg
      421 cccgttgttc gtcatcgacg agccgtgcat cgtgagcgcc agcgaggcgg cgtaacgcca
      481 caggccggcg gccccggcga tgtcgccctc ggcgagtttg atgggcttgc cggtctcttc
      541 ggcgtcgagc acggcgagcc tcgcgctgtc gcgctcgatc agttccgcaa ccttgccgac
      601 gaggatggag cgttccatcc ccgtcatctt cggccacggc ccttcgtcga aggcgtggcg
      661 agccgcgcgg gccgcagcct cgacctccct ggccccgccg gcgagaaccc ggctgacgac
      721 ctcgtcggtc ccgggcgcgc ggcgctcgat cgcctcgtcg cccgattcga ccggcgaccc
      781 gccgatgacg tgcccgacga gccgtgattc ggttgcagtg ctcatgatga acgactcctt
      841 agagtgcctt ggtgtaaaga tcggtgaggt cggccagggt gacctctctg ggattccccg
      901 gcagatcggc ctgcgtcagg gcgtcgcgcg cgagcgcggg gatggcgtcg gcgccgaccc
      961 cgacgtcgga gagatggcgg ggcacacccg tctcttcggc caggcgatgc acggcggccg
     1021 aggcccgcag cgctgccttc gccggcgcga gacccgcgac ctcttcgccg agggcggcgg
     1081 cgatgcgggc gaagcgttcc ggcgccgccg ggatgttgaa gtcgatgacg tacggcagga
     1141 acaacgcgtt ggccgtgcca tgatgcaccc cgaactgggc gcccagcgtg ttggcgagcg
     1201 cgtgcaccgc gccgaggccg gcgtcgtgaa agccgatgcc ggccatggac gaggcgacaa
     1261 gcatgttgta gagggccgtc ttgtcgccgc gggaggccgg aacgagattt ttgccgatga
     1321 tctcgatcgc cccgaggcac aacgcgtcgg tgaccggatt cgcgcccttc gcgacgtagg
     1381 cctcgatggc atgggtgagg gcatccatgc cggtggtggc cgcgatgcgg ccgggcagcc
     1441 cctcaaccac cctcgcatcg agcacggcga tcgtggcgaa catcaggtcg gagacgacga
     1501 ccatcttcac atgcgtgtcg gggttcgtga tgacggcgcc cttggtgacc tcggagccgg
     1561 agccgacggt ggtcggcacg acgacgacgg gcagcggacg cttgtcgaaa cggtcgacgc
     1621 cctcgtagtc ggcgattccg ccgccgttcg tgccgagaag cgccgccgac ttggcgacgt
     1681 cgatgctgga tccgccgccg atcgcgatga gcacgtcggc gcccgcggcg cgccgcgcct
     1741 cgtcggcggc ctcgacggtc gcgatgtcgg ggttgccgac gacgccggag aacgtttcga
     1801 cgggaaagtc ccgaccgagg attccgcgga cgaggccgtg gatgcccgcc gccatgattc
     1861 ccggatcggt cacgatgagc cctcgggtgc cgagcctcgg cagctctgcc gccacctctt
     1921 ccagggctcc ctcgcccatg accagccggg gcggggatga aaagtgcctg ctcacgaacg
     1981 atcctcttct cttgtcacca tgtatcgatt ctttgcttgc ggcacgggcg ggccgagagg
     2041 cgccaactgc ccggtacggc cggccgtcac gggccg
//
LOCUS       DASTNL010000193        11125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_326881, whole genome shotgun sequence.
ACCESSION   DASTNL010000193 DASTNL010000000
VERSION     DASTNL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326881"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFQ96_06025"
     CDS             complement(<1..616)
                     /locus_tag="VFQ96_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU0257379.1"
                     /translation="MVVVDIDHTLITDDHRLTARTVAAVRAARERGVAVVLASSRPPL
                     GMVGYLRELGLTDPEPFIALQGGLIATLAGVVRVLHSSPIPLRLARQVALAGEAAGVS
                     VNWYSGTRWFVSRLDERVRREARVVGTRPIEAQLASVRHDPEKILFIAPGGADARGLR
                     KIPLPAGVEATFSNNGYLEVTAAGVGKGSALRIVANGAGVPAAEV"
     gene            complement(736..1641)
                     /locus_tag="VFQ96_06030"
     CDS             complement(736..1641)
                     /locus_tag="VFQ96_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU0257380.1"
                     /translation="MTAIRLDGKATAAAIKDELAARVRALGERGIHPGLGTLLVGDDP
                     ASRSYVAGKHRDCAEVGIASTRIDLPGDATPGDVRQAIDRLNQDSAVTGFLVQLPLPA
                     GLDGGEALRRVAPAKDADGLHPWNLGALVLGVTGSLTTPLPCTAAGVVELLRRYDVPI
                     ASRRVVVIGRGITVGRPLGLLLTRKGVDATVTLTHSRTANLADEVRRADIVVAAAGHA
                     GLVRPEWVKPGAAVVDVGITRVGTAPSGKARLAGDVDPAVAEVAGFLSPVPGGVGLMT
                     RVMLLENIVAAAERGLRQSAPRADR"
     gene            complement(1660..2988)
                     /gene="glyA"
                     /locus_tag="VFQ96_06035"
     CDS             complement(1660..2988)
                     /gene="glyA"
                     /locus_tag="VFQ96_06035"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0257381.1"
                     /translation="MTDSTFTRPLSEVDPEIAALLEQELGRQRDFLEMIASENFVPRA
                     VLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVVEQLAIDRAKSLFHAEYANVQPHAGAT
                     ANAAVLHALAKPGDTILGMELSHGGHLTHGMRINFSGKFYNAESYQVDPKTFLVDMDA
                     VRDAALKHRPSVIIAGWSAYPRQLDFQAFRTIADEVGARLWVDMAHFAGLVATGLHPS
                     PVPYADVVSSTVHKTIGGPRSGFLLSRDAGLAKKLNSAVFPGQQGGPLMHIVAAKAVA
                     FKLAAGADFKDRQERTVRGAKIVADRLTAADSRAAGVDVLTGGTDVHLVLADLRASEL
                     DGQQAEDRLHEVGITVNRNAMPFDPRPPMVTSGLRIGTPALATRGFGDKQFTEVADVI
                     AEALKPVADLPALRERVARLAAEFPLYEGLNAFGRPLGQTAEAVPAGALR"
     regulatory      complement(3060..3163)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(3501..3800)
                     /locus_tag="VFQ96_06040"
     CDS             complement(3501..3800)
                     /locus_tag="VFQ96_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEU0257382.1"
                     /translation="MNGIKPMFNPPHPGEFITEAYLEPLGLSGRELARHLGVAPSTVA
                     RLLGGTSRLTPDMAVKLAKALGSTPRFWLTLQTNYDLWQARSVDVSDIIPINAAA"
     gene            complement(3800..4081)
                     /locus_tag="VFQ96_06045"
     CDS             complement(3800..4081)
                     /locus_tag="VFQ96_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0257383.1"
                     /translation="MIASFRHKELRLFFETGSTTGIPATRARRLRTRLAALDAVATAD
                     EIPQAPGWRVHPLKGDMDGRWSLTVTGNWRITFGFREGNAYVLDYEDYH"
     gene            4229..5122
                     /gene="purU"
                     /locus_tag="VFQ96_06050"
     CDS             4229..5122
                     /gene="purU"
                     /locus_tag="VFQ96_06050"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEU0257384.1"
                     /translation="MTALPADSAPGPASPTTTQWVLTFVCLDRPGIVHDVTGAIVDTG
                     GNITESQQFSSPDTGRFFLRLQVSSPAGHDEFLGALRPVIDRHQMTCHLDVVGRPTRT
                     LLLASTAAHCVNDLLFRHRAGQLPVEIPLILANHDVLHELADFYEVPFEYLPIASPAD
                     KAAFERRIVEVIEEHDIELVVLARYMQILSPELCERLAGKAINIHHSFLPGFKGARPY
                     RQAHARGVKLIGATAHFVTSNLDEGPIIEQNVTRVDHSDSPAELRSIGQDEESRTLRQ
                     AVTWFAQKRVLLDGARTIIFR"
     gene            5188..5541
                     /locus_tag="VFQ96_06055"
     CDS             5188..5541
                     /locus_tag="VFQ96_06055"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HEU0257385.1"
                     /translation="MDSPRAASPAGIITIAGLALDLFAFASLAAWGLATKTAPWNLVA
                     GIAAPAAAVLVWALFLSPRAVIRVDPFGRAVAEIAVTAAAALAWWSLGNPAVALVFAA
                     AAVAVGVMRGRRELA"
     gene            5538..6704
                     /gene="nagA"
                     /locus_tag="VFQ96_06060"
     CDS             5538..6704
                     /gene="nagA"
                     /locus_tag="VFQ96_06060"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU0257386.1"
                     /translation="MSPLAGEPFDLILHSAHKVDADGECADFWFAARGGRIVETGQGA
                     GWRGKPASLVVDAHGDRLTPGFIDVHGHGGSGHAFDEEAGGIEAALAVHRAHGTTRSV
                     LSLVASPLPETKAALSRIASLARHDPLILGSHLEGPYLAPARKGAHDPANLRTPSLAE
                     AEDLFDAGGGTVRQITIAPELPHALEVIGRAHERGIVAAVGHTAADHAQALAGFDAGA
                     RLLTHTFNAMNGIHHRAPGPIVAALSRPGVTLELILDGVHVHPDVARLLFAGAPGRVT
                     LITDAMAATGVGDGEYRLGSLDVTVKGGKAVLKGTDSIAGSTLTQDRALRLAIERVGL
                     EPRAAVEALTLTPARVLGRADEFGRLAPGYAADAVLFDGAWSVRSVWAAGERIA"
     gene            complement(6714..7487)
                     /gene="nagB"
                     /locus_tag="VFQ96_06065"
     CDS             complement(6714..7487)
                     /gene="nagB"
                     /locus_tag="VFQ96_06065"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU0257387.1"
                     /translation="MEVIILPSPAEVGRMAATLVVEAVTKNARAVIGLATGSSPLATY
                     AELAARAQAGEVDFSHVRGFALDEYVGIPAEHPQSYLSVIRREVVEPLGMNPDLVRVP
                     DGRAADVAAAARDYDEAIRAAGGIDVQLLGIGGNGHIGFNEPTSSFASRTRVKTLTPR
                     TRADNARFFERADQVPVHCVTQGLGTIMEARRLLLVAQGEKKAAAVKAAVEGPVSSMC
                     PASILQFHEHATVIVDEAAAGLLERHDYYTYVYANKPRA"
     gene            complement(7553..8488)
                     /locus_tag="VFQ96_06070"
     CDS             complement(7553..8488)
                     /locus_tag="VFQ96_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0257388.1"
                     /translation="MRLGIDIGGTKTDAVAVDEAGGVLARLRRQTGFGAGAVLDTAAE
                     VAIALAGEAGVSIGDFASVGVGIPGMVERAVGHVRYAVNLGVADLPLADLLSARLSGV
                     RVTVENDVNAAALGAYHTLGLEGSMGFLNLGTGLAAGVVVDGEIWHGSSGAAGEIGHI
                     PVDPHGVVCMCGQVGCLETKASGSAVARMWPVDARFPVIDLFERAGLGEEKAVRVRDE
                     LADGIAAAIRMLALTVDVDVVTIGGGLSNLGEPLHEQIGAALGRAAHHSPFLASLGLA
                     RRVRMVPAGSSIAAVGAALAATRRRERDVVGIPAD"
     gene            complement(8724..9602)
                     /locus_tag="VFQ96_06075"
     CDS             complement(8724..9602)
                     /locus_tag="VFQ96_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0257389.1"
                     /translation="MSEYTVTARRTAKSRARSYGGRRPAWKTVILSAVMYVIAFVFVF
                     PYIEMVLTSFRTDKTLLAPTVLPTAWSFSAYTTFFQTTLFGSLGISLIIAFGATAVVF
                     LVATPAAYYAARYKFKGRIAFLILVLVTQMVQPASMLIGIYVQYKTLGGLNAVWALIL
                     VNAGFNMAFAVWILTAYFGSIPKELEEAAQVQGCSRIGAIWRVTLPLAGPGVITALIF
                     TFIAAWNEFLTALTLISSPGKQPLPVTINSFMGAYGTDWQHLFSASVIATIPVIVLFA
                     LIEGKVVGGLTAGSIK"
     gene            complement(9604..10563)
                     /locus_tag="VFQ96_06080"
     CDS             complement(9604..10563)
                     /locus_tag="VFQ96_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0257390.1"
                     /translation="MSTTTASRAGTRRHDAPARRGERPRRRHGGIRRFEAALWVGPAV
                     LLIAFVVLWPIVTMFKTAFQKISPYGVTEGPAGWSNFTQLTHLPVFGLVLVNTLVWVV
                     VIVVVTMVLSLGLAQLFNLRFPGRKFTRWALIAPWAASVLMTGIVFKWMLTTGSGLLL
                     MVGHWLGLVSSVSHANPLGQAATSMPWMIAVAVFVSLPFSTYAILAGLTGIPSDIYEA
                     AAVDGASRWKRYWSVTLPLLRPAIVVATLINIMNVFNSFPIIWAMTQGAPNNSTATTT
                     VYMYILKQTNLGQSAALSVVNFVIVIILAVFFLRVSGWKAGQD"
     gene            complement(10714..>11125)
                     /locus_tag="VFQ96_06085"
     CDS             complement(10714..>11125)
                     /locus_tag="VFQ96_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257391.1"
                     /translation="KSAGVSYAFAPVVGKNGVSKSTLGVNDWIWAFKTKANHEAADKA
                     FLSFALSKENQMSLFTEYSMLPVVQSAGNEVKSENPQLKPFIDSLPTDTFYPVNQKTW
                     PQVNSAFQQSIAGAVSSNPKATLSQLQQTALGQG"
BASE COUNT         1752 a         3886 c         3825 g         1662 t
ORIGIN      
        1 ccacctcggc cgccggcacg ccggctccgt tcgcgacgat ccgcagggcg gagcccttgc
       61 ccacgcccgc ggcagtcacc tcgaggtatc cgttgttgga gaacgtcgcc tcgaccccgg
      121 cgggcagggg aatcttccgg agaccccgcg catcggcccc gcccggagcg atgaagagga
      181 tcttctccgg atcgtgccgc accgaggcga gctgggcctc gatcggccgg gtgccgacga
      241 cgcgggcctc acggcgcacc cgctcgtcga gacgggagac gaaccagcgg gtgcccgaat
      301 accagttgac gctgaccccc gcggcctcgc ccgccagggc gacctgccgg gcgagcctca
      361 gcgggatggg gctcgaatgc agcacccgca cgaccccggc cagggtcgcg atcagcccgc
      421 cctgcaaggc gatgaacggc tccgggtcgg taaggcccaa ctcgcgcagg tagcccacca
      481 tgccgagcgg cgggcgggaa gaggccagaa cgacggccac gccccgctcc cgtgcggcgc
      541 gcacggcggc gacggtacgc gccgtcaggc gatgatcgtc ggtgatcagg gtgtggtcga
      601 tatcgaccac caccatgcgg atcggcgcgt tgcccggcca ggcggagacg tggggcgtgg
      661 tgaccggagc agccgcgccc tccccgcccg gcggcccgcc gccgggcatc gcctcggcac
      721 gagcaccgcc cggcatcatc ggtcggcgcg gggcgccgac tgcctaaggc cccgctccgc
      781 cgccgcgacg atgttctcga gcaacatgac ccgcgtcatg aggcccaccc cgcccggcac
      841 gggggaaagg aacccggcca cctcggccac ggccgggtcg acgtcgccgg cgagtctggc
      901 cttgccgctc ggggcggtgc cgaccctcgt gatgccgacg tcgacgaccg ccgcgccggg
      961 cttcacccat tcgggccgca caagcccggc gtggcctgcg gcggccacga cgatgtccgc
     1021 ccggcgcacc tcgtcggcca gattcgcggt gcgcgagtga gtgagcgtga ccgtggcgtc
     1081 gacacctttg cgggtgagaa ggagccccag cgggcggccg accgtgatgc cccgcccgat
     1141 gacgacgacc cggcgggagg cgatcggcac gtcgtagcgg cgcagcagct cgacgacgcc
     1201 ggcggcggtg cagggcaacg gcgtcgtgag cgagcccgtc acgccgagca cgagcgcgcc
     1261 gaggttccag ggatgcaggc cgtcggcgtc tttcgccggg gcgacgcggc gcagcgcctc
     1321 ccctccgtcg agcccggcgg gcagcggcaa ctgcacgagg aatcccgtga cagccgagtc
     1381 ctggttgagg cggtcgatcg cctgccgcac gtcgcctggc gtcgcgtcgc ccggcaggtc
     1441 gatccgggtg gacgctatgc ccacctcggc gcaatcccga tgcttgccgg cgacgtacga
     1501 ccgggaggcc gggtcgtcac cgaccagcag cgtgccgagc ccggggtgga tgccgcgctc
     1561 gccaagcgca cgaacccggg cggcgagctc atctttgatg gcggccgcgg tggccttgcc
     1621 gtcgaggcga atcgccgtca tggccgttac cgcgcggggt cagcgcagcg cgccggccgg
     1681 cacggcctcg gccgtctgac cgagcggccg gccgaacgcg ttcagccctt cgtacagcgg
     1741 gaactcggcg gcgagcctcg ccacccgttc acgcagggcg ggcaggtcgg cgacgggctt
     1801 caacgcctcg gcgatgacgt cggcgacctc ggtgaactgc ttgtcgccga acccccgggt
     1861 cgcgagcgcc ggcgtgccga tgcgcaggcc cgaggtgacc atcggcgggc gcgggtcgaa
     1921 cggcatcgcg ttgcggttca ccgtgatgcc gacctcgtgc agccggtctt ccgcctgctg
     1981 gccgtcgagc tcgctcgccc tcaagtcggc gagcacgagg tgcacgtcgg tgcccccggt
     2041 gagcacgtcg acgccggccg cgcgcgagtc ggcggcggtc agccggtcgg cgacgatctt
     2101 cgcgccccgc accgtgcgct cctgccggtc tttgaagtct gcgccggcgg cgagcttgaa
     2161 ggccacagcc ttcgccgcga cgatatgcat gagcgggccg ccctgctggc ccgggaagac
     2221 ggcggagttc agcttcttcg cgaggccggc gtcgcggctc aagaggaagc cgctgcgcgg
     2281 cccgccgatc gtcttgtgca cggtggagga gacgacgtcg gcgtagggca ccggcgacgg
     2341 gtgcagcccc gtagcgacga gacccgcgaa atgcgccatg tcgacccaga gtcgcgcgcc
     2401 gacctcgtcg gcgatcgtac ggaatgcctg gaaatcaagc tgccgggggt aggccgacca
     2461 gcctgcgatg atgacggagg gccggtgctt cagcgcggcg tcacgcaccg cgtccatgtc
     2521 gacgaggaac gtcttcgggt cgacctggta cgactcggcg ttgtagaact ttcccgagaa
     2581 gttgatgcgc atgccgtgcg tcaggtgccc gccgtgcgag agctccatgc caagaatcgt
     2641 gtcgcccggc ttcgccaacg cgtgcagcac ggcggcgttc gccgtggcgc cggcgtgcgg
     2701 ctgcacgttc gcgtattcgg cgtggaagag gctcttggcg cggtcgatgg cgagctgctc
     2761 gacgacgtcg acatactcgc agccgccgta gtagcgtcgg cccgggtagc cctcggcgta
     2821 cttgttggtc aagacggaac cctgcgcctc gagcacggca cggggcacga agttctcgct
     2881 ggcgatcatc tcgagaaagt cgcgctgccg gccgagctcc tgctcgagca gggcggcgat
     2941 ctcggggtcg acctcggaaa gtgggcgggt gaaggtggaa tcagtcacgg tcttccttgc
     3001 tggtgtcggt tcggctctcg gtcacggatg ctgcgcccgg gcaggccgca gcggcttcgc
     3061 gttccggccc aggcgcgcgg tcgaatccat gctcgtcgct ccctgatggt gcaccatccg
     3121 cagacgggcg gaacccgccc caacgccagt cgcgacggca cgattctatc agcgtgtgcg
     3181 ccctcctcgg cctttgccgg cttctcgcta tgaagctgtc gcttctcaac ggctgggtat
     3241 aggtgacgga gtttgatgcg ggcgtcgctg gcggtgagcc gccagtcgac ttggcgtttg
     3301 tcggcgttgg cggcctgggt ccaggcagcg agtggcgctc gcccggcttc tgcctcagga
     3361 tcagacccgc ggcgacgaga ggcgcgaatc cgccggcggg gccacgcgat gaccgacacg
     3421 ggccacgact cccaagacag caacggcgag caggagcccc atcgacacgg cccagaacac
     3481 ccacccaacg gataccgcgc ctatgcggcg gcgttgatgg ggatgatgtc ggagacatcc
     3541 acagagcggg cctgccagag atcgtagttg gtctggagag tgagccagaa ccgcggtgtc
     3601 gagcccaagg ccttggcgag cttgactgcc atgtcggggg tgaggcggct ggtgccaccg
     3661 agcagacggg ccaccgtaga gggggcgacg ccgaggtgtc gagctagctc gcgcccgctc
     3721 agaccgagcg gttcgagata ggcctcggtg atgaactcgc cggggtgcgg cgggttgaac
     3781 atgggcttga tcccgttcat cagtggtaat cctcgtaatc cagcacgtag gcgttcccct
     3841 cacggaaccc gaacgtgatc ctccagttac ccgtcacggt cagtgaccat cggccgtcca
     3901 tatcaccttt caagggatgc acccgccagc ctggtgcttg cggtatctcg tcggccgtgg
     3961 ccacagcatc aagtgcagcc agccgtgtcc ggagtcgtct cgcacgtgtt gccggtatgc
     4021 ccgtggtact cccggtctcg aagaagaggc gaagctcctt atgccggaag gaggcaatca
     4081 tctcttaagt gatgcgtggc gcgcatcacg aatcacactg ctgttcggga gcgccatgcc
     4141 cagcacatcc ccgaaaggtg cggccttgca cctcgcttct gaaccgcggc aggcgggttc
     4201 ggagtcgggt ggccgatacg ctgaaggggt gactgccctg cccgccgact ccgccccagg
     4261 tcccgcctcc ccgaccacga cgcagtgggt gctcaccttc gtgtgcctcg accggcccgg
     4321 catcgtgcac gacgtgaccg gggcgatcgt cgacacgggt gggaacatca ccgagagcca
     4381 acagttctcc agccccgaca ccggtcggtt cttcttgcgc ctgcaggttt cgagccccgc
     4441 gggacacgac gagttcctgg gtgcgttacg ccccgtcatc gaccggcatc agatgacctg
     4501 tcacctcgac gtcgtcgggc gccccacgcg caccctcttg cttgcctcga cggcggcgca
     4561 ttgcgtcaac gacctgttgt tccgccaccg cgccggccag ctgccggtcg agatcccgct
     4621 gattctcgcc aaccacgacg tgctgcacga gctggccgac ttctacgagg tgcccttcga
     4681 atacctgccg atcgcgtcgc cggccgataa ggccgccttc gagcgccgca tcgtcgaggt
     4741 gatcgaagaa cacgacatcg agttggtcgt cttggcccgg tacatgcaga tcctctcacc
     4801 ggagctctgc gaacggctgg caggcaaggc gatcaacatt caccattcgt tcctgcccgg
     4861 cttcaagggg gcccggccct accggcaggc gcacgcccgc ggcgtgaagc tgatcggagc
     4921 gaccgcgcat ttcgtcacga gcaacctcga cgagggcccg atcatcgagc agaacgtgac
     4981 gcgcgtcgac cacagcgaca gcccggccga gctgcgttcg atcgggcaag acgaagagag
     5041 ccgaacgctc cgtcaggcgg tcacctggtt cgcgcagaag cgggtgttgc tcgacggcgc
     5101 gcgcacgatc atcttccgct gagcgcgccg cgagccgcac accgccgggc cggaggtcga
     5161 agacgcccac cggatagaat cgcaggcgtg gattccccgc gcgccgcgtc gcctgccggc
     5221 atcatcacca tcgccggcct cgcgctcgac ctcttcgcct tcgcgagcct cgcggcgtgg
     5281 gggctggcga cgaagacggc accgtggaac ctggtggccg gcatcgcagc acccgctgcc
     5341 gcagtcctcg tgtgggcgtt gttcctctca ccccgtgccg tgattcgcgt cgacccgttc
     5401 ggccgggcgg ttgcggagat cgccgtcacc gccgcggccg ccctggcctg gtggagcctc
     5461 ggcaatcccg cggtggccct cgtgttcgcg gcggccgccg tcgccgtcgg ggtgatgcgc
     5521 ggccggcggg agctggcgtg agccccctgg cgggcgaacc gttcgacctc atcctgcact
     5581 cggcgcacaa ggtcgacgcc gacggcgagt gcgcggactt ctggttcgcg gcacggggcg
     5641 ggcgaatcgt cgagaccggt cagggtgcgg gttggcgcgg caagccggcc tccctcgtcg
     5701 tcgacgcgca cggcgaccgg ctgacgcccg gcttcatcga cgtgcacggc cacggcggca
     5761 gcggccacgc tttcgacgag gaggccggcg gtatcgaggc ggcgctggcc gtgcaccgtg
     5821 cgcacggcac gacgcgctcc gtgttgagcc tcgtcgcgag ccccctgccc gagacgaagg
     5881 ccgcgctgag ccgcatcgcc tcgctcgcca ggcacgaccc gctgatcctc ggcagccact
     5941 tggaaggccc ctacctggcc ccggcccgca aaggcgcgca cgaccccgcc aacctccgca
     6001 caccgagcct cgccgaggcg gaagacctct tcgacgcggg cggcggaacc gtgcgccaga
     6061 tcacgatcgc gccggaactg ccccacgcgc tggaggtgat cggccgggca cacgagcgcg
     6121 gcatcgtcgc cgccgtcggc cacaccgcgg ccgaccacgc ccaggccctg gccgggttcg
     6181 acgcgggcgc ccggctcctg acccacacgt tcaacgcgat gaacggcatc caccaccggg
     6241 cacccggccc tatcgtcgcc gccctgtcac gcccgggcgt gaccctcgag ctgatcctcg
     6301 acggcgtcca cgtgcacccc gacgtggccc ggctgctctt cgccggcgcg ccggggcggg
     6361 tgacgctcat caccgacgcg atggccgcga cgggcgtcgg tgacggggag tatcggctcg
     6421 gctccctcga cgtcacggtc aagggcggca aggcggtctt gaagggcacc gattcgatcg
     6481 cgggctcgac actgacacaa gaccgcgcgc tgcgcctcgc gatcgaacgg gtcggcctgg
     6541 agccgcgggc cgcggtcgag gcgttgaccc tgacgcccgc gcgggtgctc ggccgcgcgg
     6601 acgagttcgg ccggctcgcc cccggctacg cggccgacgc ggtgctcttc gacggcgcct
     6661 ggtcggtgcg ctcggtgtgg gcggccggtg agcggatcgc ctgaccggcg ggctcaggcg
     6721 cggggcttgt tggcgtagac gtaggtgtag tagtcgtggc gttccaagag cccggcggcg
     6781 gcctcgtcga cgatcaccgt cgcgtgctcg tggaactgca agatcgaggc cgggcacatg
     6841 ctggagaccg ggccctcgac ggccgctttg acggccgccg ccttcttctc gccctgcgcc
     6901 acgaggagaa gccgacgcgc ctccatgatc gtgccgagcc cctgggtgac gcaatgcacc
     6961 ggcacctggt cggcacgctc gaagaaccgg gcattgtcgg cacgggtacg cggcgtcaac
     7021 gtcttcaccc gggtgcgcga ggcgaacgac gaggtcggct cgttgaaccc gatatgcccg
     7081 ttgccgccga tgccgagcag ctgcacgtcg atgccgcccg cggcacggat cgcctcgtcg
     7141 tagtccctgg cagccgcggc cacgtcggcc gcccgcccgt cgggcacccg aacgaggtcg
     7201 gggttcatgc cgagaggctc caccacctct cgccggatga cggagagata gctctgcgga
     7261 tgctcggccg gaatgccgac atactcgtcg agcgcgaagc cgcgcacgtg agagaaatcc
     7321 acctcccccg cctgggcgcg tgccgccaac tcggcgtagg tcgccaatgg cgacgagccg
     7381 gtcgcgagcc cgatgaccgc ccgggcattc ttcgtgacgg cctcgacgac gagcgtcgcg
     7441 gccatcctgc ccacctcggc gggcgagggc agaatgatga cctccatgct gcggcattcc
     7501 tttccggtaa tacgacgccc gccgacagcc cggtgcaggc ccgcggcgaa cgtcagtcgg
     7561 cggggatgcc gacgacgtcg cgttcacgcc gccgggtggc ggcaagggcg gcgcccacgg
     7621 ccgcgatcga actgccggcc ggaaccatgc gcacccgcct ggccaacccg agggaggcca
     7681 ggaacggcga atggtgggcc gcacgcccca atgccgcccc gatctgctcg tggagcggct
     7741 ctcccagatt actcagcccg ccaccgatcg tcacaacgtc gacgtcgacc gtcagcgcga
     7801 gcatgcggat ggccgccgcg atgccgtcgg ccagctcgtc acgcacccgc accgcctttt
     7861 cctcgccaag gccggcccgc tcgaacagat cgatcacggg gaaccgggcg tcgacgggcc
     7921 acatgcgcgc gacggcggag cccgaggcct tcgtctcgag gcagccgacc tggccgcaca
     7981 tgcatacgac gccgtgcggg tcgacgggga tgtggccgat ctcccccgcc gcgcccgagg
     8041 agccatgcca tatctcgccg tcgacgacga cgccggccgc gagcccggta ccgaggttga
     8101 gaaagcccat cgaaccttcg aggccgagcg tgtgatacgc cccgagcgct gcggcgttca
     8161 catcgttctc gacggtgacc cgcacgccgg agagccgggc ggagagcaag tcggccagcg
     8221 gcaggtcggc gacaccgagg ttcacggcat accgcacatg gcccacggcc cgctcgacca
     8281 tgccggggat gccgacgccg acggaggcga agtcgccgat cgacacgccg gcctcgccgg
     8341 caagggctat cgccacctcc gctgcggtgt cgagcaccgc acccgccccg aacccggtct
     8401 gcctgcgcag gcgtgccaag acgccccccg cctcgtctac ggcgacggcg tcggtcttcg
     8461 tgccgccgat atcgattccg agcctcacac cgtttctcct gtcacttctt cacttctgtc
     8521 tgcccttcgg ccttcttacg gccgtggcct taccggccgc ggagcgccat gttcgttgcc
     8581 gacgcccggc gacacgcatc ccccctccgg aaccgcgcat cgcgccgccc gccacgccgc
     8641 ccgcggccgg gcacgtcggg tgctcggcgc ctgccgaacg cacgccgaac gccgcacccc
     8701 acgggcacgc ccgagtggca ggcctacttg atcgaccccg cggtcaggcc gccgacgact
     8761 ttgccctcga tcaacgcgaa cagcacgatc acggggatcg tcgcgatgac ggaggcagag
     8821 aagaggtgct gccagtcggt gccgtatgcg cccatgaagc tgttgatcgt cacaggcaac
     8881 ggctgtttgc ccggcgacga gatgagggtc aacgccgtca ggaactcgtt ccaggcggcg
     8941 atgaaggtaa agatcaacgc cgtgatgacc cccggccccg ccaacggcaa ggtgacccgc
     9001 cagatggcgc ctatccggct gcagccttgc acctgcgccg cctcttccag ttctttcgga
     9061 atcgaaccga agtacgcggt cagaatccat acggcgaatg ccatgttgaa tcccgcgttg
     9121 acgagaatca aggcccagac ggcgttcaac ccgccgagcg tcttgtactg cacatagatg
     9181 ccgatgagca tcgacgcggg ctgcaccatc tgggtgacga gcaccaggat caggaacgcg
     9241 atccggccct tgaacttgta ccgtgccgcg tagtaggcgg ccggcgtcgc gaccaggaac
     9301 acgacggcgg tcgcgccgaa ggcgatgatc agcgagatgc cgaggctccc gaagagcgtg
     9361 gtctggaaga acgtcgtgta ggccgagaac gaccacgccg tcggcagcac ggtcggcgcc
     9421 agcagcgtct tatcggtgcg gaacgaggtc agcaccatct cgatgtaggg aaagacgaag
     9481 acgaacgcga tgacatacat caccgctgaa aggatgacgg tcttccaggc cggccggcgg
     9541 ccgccatacg agcgcgcacg gctcttggcg gtgcgccgcg cggtcacggt gtactcagac
     9601 atgtcagtct tgccccgcct tccagccgct gaccctcaag aagaacacgg ccaagatgat
     9661 gacgatcacg aagttgacga ccgataaggc tgccgactgg ccgagattcg tctgcttgag
     9721 gatgtacatg tagaccgtcg tcgtggccgt cgagttgttg ggtgccccct gcgtcatcgc
     9781 ccagatgatc ggaaagctgt tgaagacgtt catgatgttg atgagcgtcg ccacgacgat
     9841 cgccggccgc aggagcggca gtgtgacgct ccagtaccgc ttccaccgag aggcgccgtc
     9901 tacggcggcc gcctcgtaga tgtcgctcgg tatgccggtg aggccggcca agatcgcgta
     9961 ggtcgagaag ggcagggaga cgaagaccgc gacggcgatc atccacggca tcgacgtggc
    10021 cgcctgcccg agcggattgg cgtgggagac ggaagagacg aggccgagcc agtgccccac
    10081 catgaggagc agcccggagc cggtggtcag catccatttg aagacgatgc ccgtcatcag
    10141 caccgacgcc gcccacgggg cgatcagcgc ccaccgggtg aacttgcggc cgggaaagcg
    10201 caggttgaag agttgggcga ggcccagcga gagcaccata gtgacgacga cgatgacgac
    10261 gacccagacc agtgtgttca ccagaacgag gccgaagacc ggcaggtgcg tcagctgggt
    10321 gaaattgctc cagccggccg gcccctccgt cacgccatag gggctgatct tctgaaaggc
    10381 ggttttgaac atcgtgacga tcggccacag cacgacgaag gcgatgagca gcaccgccgg
    10441 gccgacccac aaggccgcct cgaagcgtcg tatcccgccg tgtcgtcgcc gcggtctctc
    10501 gccgcgccgt gcgggcgcgt catgtcgtcg cgtcccggcc ctcgaggccg tcgtggtgct
    10561 catgggatgt actttcgttc gtgttcgtac ggtggcccct gccgcaggtc tccgatcggg
    10621 tgatcatgcg gcgaggcggg tgtggccggg gtgttcggtg caggcccggg tggacccgca
    10681 ccgaacgcct ctgccggtcg acgcctggcg ggctcagccc tgcccgagcg ccgtctgctg
    10741 gagctgcgag agggtcgcct tcgggttgct gctgacggcg cccgcgatgc tctgctggaa
    10801 ggcggagttc acctgtggcc acgtcttctg gttcaccggg tagaaggtgt cggtcggcag
    10861 cgagtcgatg aagggcttca gctgcgggtt ctcgctcttg acctcgttgc cggcactctg
    10921 cacgacgggc agcatgctgt actcggtgaa caacgacatc tggttctcct tcgacaaggc
    10981 gaagctcagg aacgccttgt cggcggcctc atggttcgct ttcgtcttga aggcccagat
    11041 ccagtcgttg acgccaaggg tgctcttgct gacgccattc ttgccgacga caggggcgaa
    11101 ggcatagctc acgcccgccg acttc
//
LOCUS       DASTNL010000194         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1621899, whole genome shotgun sequence.
ACCESSION   DASTNL010000194 DASTNL010000000
VERSION     DASTNL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621899"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="VFQ96_06090"
     CDS             <1..1667
                     /locus_tag="VFQ96_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0257392.1"
                     /translation="LVGQLDTQLDAASQPNNIKSCLASPASGDDEIASDGSTCAYFVV
                     LYNAAGDRIGVNWKNEPAGHLPQVPARVSLAMGLEMSRHDFNLPSANNETTYRATATI
                     VPLISADQENGTVIVAASTQQIDTVMSSYLTIFLGFGILVLTVGAGLTRMVVGTTFGP
                     LREVEHTAAQIAAGDFSQRMEGATPNTEVGRLSRSLNIMLSRIDDALRGRAKTIAQMR
                     RFVGDASHELRTPLVSLRGYAELYRMGALQKPEEIASAMDRIEREATRMGLLVQDLLQ
                     LARLDEEKPLNMTRVDLVPIARDAAMDVTASAPSRTVRVETPPDVAAAEDPAAAAAPP
                     TPATPAAPTGPIAFAGATLARLRSRGKASRQGDAGIPGAPATEPKPLAGEAPPPAPPE
                     ATVAVLADENKIRQVITNLVGNALRYTDQGSPIEIRVQKDPAAARAVLEIVDHGEGIP
                     EQLRDKIFQRFWRADTSRTRETGGSGLGLAIVASIVAAHHGTVEARETPGGGATFRIV
                     LPLAPERPEAHGPHAATASGPYAGPQAGANPGTNSPAGPATEPPRH"
BASE COUNT          348 a          752 c          669 g          261 t
ORIGIN      
        1 ttctcgtcgg acaactcgac acgcagctcg acgcggccag ccagccgaac aacatcaaga
       61 gttgcctggc ctcccctgcg agcggcgacg acgagatcgc ctctgacggc agtacctgcg
      121 cgtatttcgt cgtcttgtac aacgctgccg gcgaccggat cggcgtcaac tggaagaacg
      181 aacctgccgg ccatctgccg caggttcccg cgagggtgag cctcgccatg ggcctcgaga
      241 tgagccgaca cgacttcaac ctgccgagcg ccaacaacga gaccacctac cgggcgaccg
      301 cgacgatcgt gcccctgatc tcggccgatc aagagaacgg cacggtcatc gtcgcggcct
      361 cgacccagca gatcgacacc gtcatgtcga gctacctcac gatcttcctc ggcttcggca
      421 tcctggtgct caccgtcggc gccgggctga cccgcatggt ggtgggcacc acattcggcc
      481 ccctgcgtga agtggagcat acggccgctc agatcgccgc cggcgacttc agccagcgga
      541 tggagggcgc gacccccaac accgaggtgg ggcgcctcag ccgatcgctc aacatcatgt
      601 tgagtcgcat cgacgacgcc ttgcggggcc gggcgaagac gatcgcgcag atgcgtcgtt
      661 tcgtcggcga cgccagccac gagctgcgca ccccgctcgt ctcgctgcgt ggctacgccg
      721 agctgtaccg gatgggcgcc ttgcagaagc ccgaagagat tgcttcggcg atggaccgca
      781 tcgaacgcga ggcgacgcgg atgggcctgc tcgtgcaaga ccttctgcaa ctcgcccggc
      841 tcgacgagga gaagccgctc aacatgaccc gtgtcgacct ggtgcccatc gcccgagacg
      901 ctgcgatgga cgtgaccgcg tcagctccca gccgcacggt gcgggtcgag acgccccccg
      961 acgtggccgc ggcggaggac ccggcggcgg ccgccgcccc accgacaccc gcgaccccgg
     1021 ccgcgccgac cgggccgata gcgttcgccg gcgcgacact ggccaggctg cgctcccgcg
     1081 gcaaggcctc ccggcaaggc gatgccggca ttccgggggc ccccgcgacc gagcccaagc
     1141 ctctcgccgg cgaggcccca ccgcctgccc cacccgaggc gaccgtggca gtcttagccg
     1201 acgagaacaa gattcggcag gtcatcacca acctcgtcgg caacgcgttg cgctataccg
     1261 accagggcag cccgatcgag atccgcgtgc agaaagaccc ggctgcggcc cgcgccgtgc
     1321 tggagatcgt cgatcacggc gaaggcatcc ccgagcagct gcgcgacaag atctttcagc
     1381 gtttctggcg cgccgacacc tcccgcacgc gggagacggg cggcagcgga ctgggtctcg
     1441 ccatcgtcgc ctcgatcgtc gctgcccacc acggcacggt cgaggcccgg gagaccccgg
     1501 gcggcggcgc gacgttccgc atcgtcttgc cattggctcc cgaacgcccg gaggcccacg
     1561 ggccgcacgc cgccacggcg tcgggcccat acgcggggcc ccaggccggg gcgaacccag
     1621 gcacgaactc acctgccggg cctgcgacgg aaccgccgag gcattgaggg gcccgcagag
     1681 acggcgacgg acacccgccc tgccagcacg ctccggcaac ggagcagcca cggcgctacg
     1741 ggggcacgcg gcccgtgggt aggccatgca tggccgagga ggctctggtc gtgctacgcc
     1801 acgccgtgcc gtccagcgcc cggccccacc gtgactgcgg cgctgcagcg gcgatgggca
     1861 acccgccgcg acccctccac gggattcgca gggtaagagg ctctacccgt tctcacccag
     1921 gccgccgacg gaatagcccg aggcccgtag cctcatcgca tcggagcaca ggagggccta
     1981 tgagcagatt ccaggtagac agcgatgagg tgctgggcgc gacgagtgcc
//
LOCUS       DASTNL010000195         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_145799, whole genome shotgun sequence.
ACCESSION   DASTNL010000195 DASTNL010000000
VERSION     DASTNL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145799"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFQ96_06095"
     CDS             <1..491
                     /locus_tag="VFQ96_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0257393.1"
                     /translation="VAYVGDGANNMAQSYLLAGATAGMHVRIAAPAGYRPSAAVVADA
                     RAIADRTGGSVQVGGEPAEAAAGADVIVTDTWVSMGQEAEKEKRQKEFRGYCVDDALI
                     GRAAPDVIFLHCLPAYRGYEVAASVIDGPHSVVWDEAENRLHAQKALLAWLLTRQAPA
                     LH"
     gene            604..1800
                     /locus_tag="VFQ96_06100"
     CDS             604..1800
                     /locus_tag="VFQ96_06100"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0257394.1"
                     /translation="MAERVVLAYSGGLDTSVGIGWLHDATGKDVVALSVDVGQGGEDL
                     EAIRQRALACGAVDAVVVDAKEEFASDYLMPALKANALYQKRYPLVSALSRPLIAKHL
                     ARTAQRLGADSIAHGCTGKGNDQVRFEAAIAAIAPELTSLAPVRDFALTRDKAIVYAA
                     EHNLPIEQSKKSPYSIDQNLWGRAVETGFLEDPWNAPIEDLYTYTQDPAAPREPTEVT
                     ITFTEGIPTAIDGDEVSPLEAVLRMNSLAGGQGVGRIDMVEDRLVGIKSREVYESPAG
                     IALIAAHEELENLTVERDLARYKRTVEAKWAEAVYDGLWFSGLKRALDVFVDETQRHV
                     SGDIRLRLHAGTAVVTGRRSAQSLYDFNLATYDTGDTFDQSLAKGFIELWSLPSKIAA
                     RRDLRA"
     gene            1898..3352
                     /gene="argH"
                     /locus_tag="VFQ96_06105"
     CDS             1898..3352
                     /gene="argH"
                     /locus_tag="VFQ96_06105"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0257395.1"
                     /translation="MPDGSGSKEGSGAVREGSLWGGRFARGPAPELAELSRSTQFDWR
                     LAHYDLAGSRAHARALARAGYLHDDELQAMLAALESLEADLTDGVFRAHPDDEDVHGA
                     LERGLIERAGAELGGRLRAGRSRNDQIATLIRMYLRDHSASLARLLLGLADALEDQAA
                     RHPKAIMPGRTHLQHAQPVLLAHHLLAHCWPLVRDLERIRDWRRRNDVSPYGSGALAG
                     TTLGLDAGLVARELGFASSTPNSIDGTAARDLVAEFAYLCAQVGVDLSRLAEEIVLWN
                     TQEFHFVTLDDAYSTGSSIMPQKKNPDIAELARGKSGRLIGNLTGLLVTLKGLPLAYN
                     RDLQEDKEPVFDSIDTLEVLLPAFTGMVRTMAFDEERMALLAPQGFTLATDVAEWLVK
                     RHVPFRRAHEVTGALVRAAEQRGTDLAGLRDDDLRHVAPELTPEVREVLSVAGAVAGR
                     SGTGGTAPERVAEQQAFLRQRLAALAGELGVAAS"
     gene            3505..4152
                     /locus_tag="VFQ96_06110"
     CDS             3505..4152
                     /locus_tag="VFQ96_06110"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU0257396.1"
                     /translation="MRAFLHATALEVAPLLLGAVISHETTEGTVGVMLTEVEAYLGDG
                     TDPGSHAYRGKTARNAPMFGEPGHLYAYFTYGMYTCGNIVCSPDGTASGVLMRAGRVV
                     EGVELARSRRSTSKKDHDLARGPALLAMALGVTLADKGADLLTSPFRLELAPAPLEYR
                     SGPRTGLSGPGGTEDFPWRFWLPDEPSVSAYKRHKHAGEEVRRGRAAGPGSAAGR"
     gene            4165..>4570
                     /locus_tag="VFQ96_06115"
     CDS             4165..>4570
                     /locus_tag="VFQ96_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU0257397.1"
                     /translation="MPDSSPLAAQSNDDSFGDVWEELSWRGLIRVSTDRAALKALLAG
                     SPITFYCGFDPTAPSLHLGNLVQLLVMRRLQLAGHRPLGLVGGSTGLIGDPRPTAERT
                     LNTKDVVAEWVGYLQQQVSRFLAADGDNGVTLV"
BASE COUNT          701 a         1638 c         1572 g          659 t
ORIGIN      
        1 tcgtcgccta cgtcggcgac ggcgccaaca acatggccca gtcgtacctt ctcgccggcg
       61 ccaccgccgg catgcatgtg cgcatcgcgg cgccggccgg ctatcggcct tccgcggccg
      121 tcgtggccga cgcccgggcg atcgccgacc gcaccggcgg cagcgtgcag gtgggcggcg
      181 agccggccga ggcggctgcc ggcgccgacg tcatcgtcac cgacacctgg gtctcgatgg
      241 ggcaggaggc cgagaaggag aagcggcaga aggagttccg cggctactgc gtcgacgatg
      301 ccctcatcgg ccgcgcggcg cccgatgtga tcttcttgca ctgcctgccc gcctaccgcg
      361 gctacgaggt ggcggcctcg gtgatcgacg gaccgcacag cgtcgtctgg gacgaagcgg
      421 agaacaggct gcacgcgcag aaggcgcttt tggcgtggct cctgacccgc caggcgcccg
      481 ccctacactg aacacaccac ccttcccgcg tgcgggccgg ggcccccgca cgcgggtcgg
      541 cagacatcca tccggcaact attcggcgat tctcgtggtc gccgaccacc ccaaaggaga
      601 atcatggcgg aacgcgtcgt cctcgcgtac tcgggcggac tcgacacctc ggtcggcatc
      661 ggctggctgc acgacgccac cggcaaggac gtcgtagccc tctcggtcga cgtcggtcaa
      721 ggcggcgaag accttgaggc gatccgtcaa cgggccttgg cctgtggtgc ggtcgatgcg
      781 gtcgtcgtcg acgccaagga ggagtttgcc tccgattacc tcatgcctgc gctcaaggcg
      841 aacgcgctct accaaaagcg ctacccgctt gtctccgcgc tcagccggcc gctgatcgcg
      901 aagcacctgg cccgcacggc gcagcggctc ggcgccgaca gcatcgcgca cggctgcacc
      961 ggcaagggca acgaccaggt gcggttcgag gcggccattg cggcgatcgc cccagagctg
     1021 acctcgctgg cccccgtgcg cgacttcgcc ctgacccgcg acaaagcgat cgtctacgcg
     1081 gcggaacaca acctgccgat cgagcagtcg aagaagagcc cctattcgat cgaccagaac
     1141 ctgtggggcc gcgcggtcga gacgggattc ctcgaagacc cctggaacgc acccatcgaa
     1201 gacctgtaca cctacaccca agacccggcg gcgccgcgtg agcccaccga ggtgacgatc
     1261 accttcaccg aggggatccc caccgcgatc gacggcgacg aggtcagccc gctggaggct
     1321 gtgttgcgga tgaacagcct cgccggcggc cagggtgtcg gccgcatcga catggtcgaa
     1381 gaccgcctcg tcggcatcaa gagccgcgag gtgtatgagt cgcccgccgg catcgcgctc
     1441 atcgcggccc acgaagagct cgagaacctc acggtggaac gcgacctggc ccgctacaag
     1501 cgaaccgtcg aggcgaagtg ggcggaggcg gtctacgacg gcctgtggtt ctcaggcttg
     1561 aagcgcgccc tcgacgtgtt cgtcgacgag acccagcggc acgtcagcgg cgacatccgg
     1621 ctgcggctgc acgcggggac cgcggtcgtc actggccggc gcagcgccca gagcctctac
     1681 gacttcaacc tcgccaccta tgacaccggc gatacgttcg atcagtcgct ggccaagggc
     1741 ttcatcgagc tgtggtcgtt gccgagcaag atcgccgccc gccgcgacct gcgggcgtga
     1801 gggcgttcgg cggaggcggc cccgcggcgc gggccgcgga aggaaggaga ggccgggcgt
     1861 gagcgaggcc gagaacgacg acgggcagcc gcaggcggtg cccgacgggt cggggtcgaa
     1921 ggagggttcg ggggccgtgc gcgagggctc tctctggggc ggccgctttg cccgcggccc
     1981 cgcacccgag ctcgccgaac tgagcaggtc gacgcagttc gactggcgcc tggcgcacta
     2041 cgacctggcc ggctcccggg cgcacgcccg tgccctcgca cgcgccggct acctgcacga
     2101 cgacgagctc caggcgatgc tcgcggccct cgagagcctg gaggccgacc tcaccgacgg
     2161 cgtgttccgg gcgcaccccg acgacgaaga tgtgcacggc gccttggagc gcggtctcat
     2221 cgagcgcgcc ggcgcagagc tcggcggccg gttgcgggcc ggccgtagcc gcaacgatca
     2281 gatcgcaacg ctcatccgca tgtacctgcg cgaccattcc gcgagcctcg cccgcctcct
     2341 cttgggcctg gccgacgcgt tggaggatca ggccgcgcgg catccgaagg cgatcatgcc
     2401 ggggcgcacc cacctgcagc acgcgcaacc ggtgcttctg gcccaccacc tgttagccca
     2461 ctgctggccg ctcgtccgag acctcgaacg gatccgcgac tggcgtcgcc gcaacgacgt
     2521 ctcaccgtac ggctccggtg cgctcgcggg aacgacgctc ggcctcgacg cgggcctggt
     2581 cgcccgggag cttggcttcg cctccagcac gccgaactcg atcgacggca cggcggcgcg
     2641 cgatctcgtt gccgagttcg cctacctctg tgcccaggtc ggcgtcgacc tgtcccgcct
     2701 cgccgaggag atcgttttgt ggaacaccca ggagttccac ttcgtcaccc tcgacgatgc
     2761 ctattcgacg ggctcgtcga tcatgccgca gaagaagaac cccgacatcg ccgagttggc
     2821 gcgcggcaag tcgggccggc tcatcggcaa cctcacgggt ctgctcgtga ccctgaaggg
     2881 gctgccgttg gcctacaacc gcgatctgca agaagacaaa gagccggtct tcgactcgat
     2941 cgacaccctg gaggtgctgc tgccggcgtt caccggcatg gtgcgcacga tggcgttcga
     3001 cgaggaacgc atggccctcc tcgccccgca aggtttcacc ctggccacgg acgtggccga
     3061 atggctcgtg aagcggcacg tgccgttccg ccgggcgcac gaggtgacgg gcgccctggt
     3121 gcgggcggcg gagcaacgcg gcaccgatct cgccggcctt cgcgacgacg accttcggca
     3181 cgtggcgccc gagttgacgc ctgaagtgcg ggaggtgttg agcgtcgccg gcgcggtcgc
     3241 cgggcgttcg ggcaccgggg gcacggcgcc cgagcgggtc gcggaacagc aggcgttcct
     3301 gcgccagaga cttgccgcgc tcgccggcga gctcggggtg gcggcgtcgt gacggcgcga
     3361 cctgcagtgc ccaccaccgc cacctcccgt cgccacgcgc accccgggcg ggccggcgcg
     3421 acgcaagaac ggcgccgtgg cgcctctccg gcggggcccc tggcccgcga agacgaagca
     3481 cgacgggggt tcgatccgcc gaccatccgt gccttcctgc acgccaccgc gctcgaagtc
     3541 gccccgctcc tcttaggggc ggtgatctcc cacgagacga cggagggcac ggtgggggtg
     3601 atgctcacgg aggtcgaggc atatctcggc gacggcaccg accccggctc gcacgcctac
     3661 cgcggcaaga cggcaaggaa cgcgccgatg ttcggggagc ccggccactt gtacgcctat
     3721 ttcacctacg gcatgtacac ctgcggcaac atcgtctgct cgcccgacgg cacggcctcg
     3781 ggtgtcctga tgcgggccgg ccgggtggtg gagggggtcg aactggcccg gtcgcgtcgc
     3841 tcgacgagca agaaagatca tgacctggcc cgggggcccg cgctcctcgc gatggcgttg
     3901 ggcgtcaccc tcgccgacaa gggggccgac cttctgacga gcccgttccg actcgagttg
     3961 gcgccggcgc ccctcgagta tcgatccggg ccccgcaccg ggctgagcgg ccccggcggc
     4021 acggaggact tcccgtggcg attctggctg cccgacgagc cttcggtgtc ggcctacaag
     4081 aggcacaaac acgcgggaga agaggtccga cgtggccgtg ccgccggccc cggttcggcc
     4141 gccggccgct gatactgtaa cggtgtgccc gactcgtcgc cccttgccgc ccagtccaac
     4201 gacgactcct tcggcgatgt ctgggaggag ctctcctggc gcgggctgat ccgcgtctcc
     4261 accgatcgcg cggcattgaa ggcgcttctt gcgggctcgc cgatcacgtt ctattgcggg
     4321 ttcgatccca ccgcgcccag cctgcacctg ggcaatctcg tgcagctcct cgtcatgcgg
     4381 cggcttcagc tggccggtca tcgtccgctg gggctcgtgg gcgggtcgac ggggctcatc
     4441 ggcgatccgc gacccaccgc cgaacgcacg ctcaacacca aggatgtcgt cgccgagtgg
     4501 gtgggctacc tgcagcagca ggtctcccgc ttcctcgccg ccgacggtga caacggcgtg
     4561 accctcgtga
//
LOCUS       DASTNL010000196         5948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1441744, whole genome shotgun sequence.
ACCESSION   DASTNL010000196 DASTNL010000000
VERSION     DASTNL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441744"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1119
                     /locus_tag="VFQ96_06120"
     CDS             310..1119
                     /locus_tag="VFQ96_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU0257398.1"
                     /translation="MASRNPAFSKSPAFRPNNRAAFAGSVATTSGDLETLYQSPAATA
                     ADTDRMTYDDTIGKTALIFLVLLAASGIGWVFPALAIPGAIAGFVLAMINAFKRVPSA
                     PLIFAYAATEGLFVGGISHLFEQMWGGIVIQAVLGTLVVFGVTLALFASGKIRASKRA
                     TKIFLIAMVGYVIFSLVNVGLMMFGGVSSSLAFGLRSQATIFGIPLGIVIGLAAILMA
                     AYSLVLDFDFIKNGVANRAPRAYGWYGAFGLMVTIVWLYIEMLRLFAIARN"
     gene            complement(1414..4218)
                     /gene="secA"
                     /locus_tag="VFQ96_06125"
     CDS             complement(1414..4218)
                     /gene="secA"
                     /locus_tag="VFQ96_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU0257399.1"
                     /translation="MASVLERVLRFGEGRTLRRLQNYAKAVGALEDDFAALSDEDLRG
                     ETALLRERHGAGESLEDLLPEAFAAAREAAKRTLGMRPFDVQVMGGAALHLGNIAEMK
                     TGEGKTLVATLPAYLNAITSRGVHIVTVNDYLAGYQSELMGRVFRALGMTTGVILSGQ
                     NPQERREQYAADVTYGTNNEFGFDYLRDNMAWQTADMVQRGHYFAIVDEVDSILIDEA
                     RTPLIISGPSSGEANRWFTEFASLAARLEVGVDYEVDEKKRTVGVLEPGIEKVEDYLG
                     IENLYESANTPLISFLNNAIKAKALFKRDKDYVVLNGEVLIVDEHTGRILVGRRYNEG
                     VHQAIEAKEGVEVKAENQTLATVTLQNYFRMYTKLSGMTGTAETEAAEFMSTYKLGVV
                     TIPTNKPMIRKDMPDFVYKHEEAKFAQVAEDIVARNEKGQPVLVGTTSVEKSEYLSRL
                     LAKKGVRHEVLNAKNHAREASIVAQAGRLGAVTVATNMAGRGTDIMLGGNAEFLAVAE
                     LERRGLNPTETPEEYEAAWDEVYARVTAEVEAEAEEVRAAGGLYVLGTERHESRRIDN
                     QLRGRSGRQGDPGESRFYLSLTDDLMRLFNSSAAQSLMNRSNVPDEMAIESRVVSRAI
                     RSAQSQVEARNAEIRKNVLKYDDVLNRQREAIYGDRRRILEGDNLSDRVHTFLTATID
                     AIIDDHTAEGNTGDDWDLEALWQELKTLYPVGITVEEITDAAGSRGQVTREELKREVG
                     SDARIAYQRREESLGAPAMRELERRVVLSVIDRRWRDHLYEMDYLKDGIGLRAMAQRD
                     PLVEYQREGFGMFQQMMEQIREESIGFLFNLEVQVTAQDAGEVEVGAKGLDQSASGEE
                     ALSYSAPTDDGEVEVRDRAGRLEQAATARARRAAVQEDAAPQQPAGRGAFGEPVAADS
                     SAEAQPQNRAERRAHARRRA"
     gene            complement(4425..5084)
                     /gene="raiA"
                     /locus_tag="VFQ96_06130"
     CDS             complement(4425..5084)
                     /gene="raiA"
                     /locus_tag="VFQ96_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU0257400.1"
                     /translation="MELEIFGRNLGITDRFREYVTGKSEKISRLSDRALALEVKVSRH
                     REKADKTSLEDRVELTLIGPGPVVRSEATGFDKYSAFDTAQTRLVERIRRVKDRRKVH
                     RGGMKRPTSLREASSKSFGGAGIVPAPGDLLEKVRTGTIPIVGEMTEENADDVYGPVV
                     IRRKVFQAMPMSVDDALYRMELVGHDFYLFVDSETGRPSVVYRRKGWDYGLIALGEEA
                     A"
     gene            complement(5157..5870)
                     /locus_tag="VFQ96_06135"
     CDS             complement(5157..5870)
                     /locus_tag="VFQ96_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU0257401.1"
                     /translation="MATWWREACDLLLPVSCAGCGASGGSLCTSCLRAMTPRVVRADL
                     DAGLPVWAAGRYEGTLRRVILAYKDGGRTDCGPALAGLLRAALRAALTQVPAGEVVAR
                     QGIRLTTVPSTRAAWRRRGYRPVPRLASHAGLKPAQILRPLRQSVDQAALDRAAREEN
                     RSGSLAARRVPAGSAWLLIDDIVTTGATTREVARAIGAAGGGLLGVVALAYTPRRVAD
                     ARDTPPVGEGGRGAWPALP"
BASE COUNT          878 a         2119 c         1957 g          994 t
ORIGIN      
        1 cgcccggtac ccggcgtcag gcccgacgcc gacaccgatc gtccgcgaca tggggcccga
       61 cggccgggcg gcgacgcacg aacgccggcc tcagcctcac ggcgccctac gacgacagca
      121 accggtgagt gtgccttacg catgcccaag gccccggagg ccgccacatc atcggtgcat
      181 agtggactcc cgccctccgg taggcggatt gccaggcgga atcacggccc gctccggtag
      241 gttaagaggg atacggatcg gcgcctcgcg cccgtcacgc atgtcgaccg atgggagtcg
      301 gagaagatca tggcatcacg caatcctgcc ttctcgaaga gtccggcatt ccggccgaac
      361 aacagggccg cattcgcggg cagcgtcgca acgacgagcg gcgacttgga gacgctttac
      421 cagtcgccgg cggcgaccgc ggccgatacc gatcgcatga cctacgacga cacgatcggc
      481 aagaccgcgc tgatcttcct cgtgctcctc gccgcgtccg gcatcgggtg ggtctttccg
      541 gcgctggcga tccccggcgc gatcgctggc ttcgttctgg cgatgatcaa cgcgttcaaa
      601 cgcgtgccgt cagcgccact catcttcgcg tatgcggcga cggagggcct gttcgtcggc
      661 ggcatctccc acctcttcga gcagatgtgg ggtggcatcg tcatccaggc ggtgctcggc
      721 acgctcgtcg tgttcggcgt gaccctggcg ctcttcgcga gcggcaagat ccgggcgtcg
      781 aagcgcgcga cgaagatctt cctcatcgcg atggttggct acgtcatctt cagcctcgtc
      841 aacgtggggc tcatgatgtt cggcggggtg agcagctcgc tcgccttcgg cctgcgctcc
      901 caggcgacga tcttcggcat tccgctgggc atcgtgatcg gcctcgccgc gatcttgatg
      961 gcggcctatt cgctcgtctt agacttcgac ttcatcaaga acggcgtcgc caaccgggcg
     1021 ccgcgcgcct acggctggta tggcgccttc ggcctcatgg tcacgatcgt gtggctctac
     1081 atcgagatgc tgcgcctctt cgcgatcgcc cgaaactagc tgcgcggccg acgcgacacg
     1141 ccgcgaccgg cactccccga tcccaccccc tacgacacgc cccgtgctcg aggggcgggg
     1201 tgaagtgtgg agcggtggct gccggccggg tggcgcgaca caccccgtac gcggggtcag
     1261 tggcaggggg cagcggcaga ggcgacaacc gtacgcgggg ggcagaggcg acgcccctgc
     1321 ccggtttcag cccaccgcgg ggtgtggcgg cggccgccca cgcacggcgc ttcgccgatg
     1381 gggcggtgtg tgccgggcgg ggccggcggg tgctcaggct cggcggcggg cgtgggcgcg
     1441 gcgctccgcg cgattctggg gctgggcttc ggccgacgaa tcggcggcga ccggctcgcc
     1501 gaaggccccg cggccggcag gctgctgcgg ggccgcatcc tcctgaaccg cggcacggcg
     1561 ggcgcgcgcg gtcgcggcct gctccagccg gccggcccga tcacgcacct ccacctcgcc
     1621 gtcgtccgtc ggcgcggagt agctgagcgc ctcttcgccc gaggccgact ggtcgaggcc
     1681 cttcgccccc acctccacct cgccggcgtc ttgcgcggtg acctgaacct ccaggttgaa
     1741 gaggaagccg attgactcct cgcggatctg ctccatcatc tgctggaaca tgccgaagcc
     1801 ctcacgctgg tattccacca gcgggtcgcg ctgcgccatg gcgcgcaggc cgatgccgtc
     1861 tttcaggtaa tccatctcgt acaggtggtc gcgccaccga cggtcgatga cgctcaagac
     1921 gacccggcgc tccagttcgc gcatcgcggg cgcgccgagg gactcctcgc ggcgctgata
     1981 cgcgatgcgc gcgtcggagc cgacctcgcg cttcaactcc tcccgagtga cctggccacg
     2041 gctgccggcg gcgtcggtga tctcctccac cgtgatgccc accgggtaca gcgtcttcag
     2101 ctcttgccac agagcctcga gatcccagtc gtcgccggtg ttgccctcgg ccgtgtggtc
     2161 gtcgatgatc gcatcgatcg tcgcggtcag gaacgtgtgc acccggtcac tcaggttgtc
     2221 gccctcgagg atgcgccgcc ggtcgccgta gatcgcctca cgctgccggt tcaagacgtc
     2281 gtcgtacttc agcacgttct tgcgaatctc ggcgttgcgc gcctccacct gcgactgggc
     2341 gctgcggatc gcacggctga cgacgcgaga ctcgatcgcc atctcgtcgg gcacgttact
     2401 gcggttcatg aggctctgcg ccgcggacga gttgaacaag cgcatcaggt cgtcggtgag
     2461 cgagaggtag aagcggctct cgcccgggtc gccctggcgg ccggaacgac cccgcagctg
     2521 gttgtcgatg cgccgcgact cgtgccgttc ggtgccgagc acgtagaggc cgccggctgc
     2581 gcggacctcc tcggcctccg cctcgacctc cgcggtgacg cgggcgtaaa cctcatccca
     2641 cgccgcctca tactcctccg gagtctcggt cgggttgagg ccccggcgtt ccaactcggc
     2701 gacggcgagg aactccgcgt tgccgccgag catgatgtcg gtgccgcggc ccgccatgtt
     2761 cgtggcgacc gtcaccgctc ccagccggcc cgcctgcgcg acgatcgagg cctcacgcgc
     2821 gtggttcttc gcgttcagca cctcgtggcg cacgcccttc ttcgccaaga gccgcgacag
     2881 gtactcgctc ttctcgacgc tcgtcgtgcc gacgagaacg ggctggccct tctcgttgcg
     2941 cgcgacgatg tcctccgcga cctgcgcgaa cttcgcctcc tcgtgcttgt aaacgaagtc
     3001 gggcatgtct ttgcggatca tcggcttgtt cgtgggaatc gtcacgacgc ccagcttgta
     3061 cgtcgacatg aactcggccg cctcggtctc ggcggtgccg gtcatgccgg agagcttggt
     3121 gtacatgcgg aagtagttct gcaacgtgac ggtcgcgagg gtctggttct cggccttgac
     3181 ctcgacgccc tccttggcct cgatcgcctg atggacgccc tcgttgtagc gtcgaccgac
     3241 aaggatgcgg ccggtgtgct catcgacgat cagcacctcg ccgttcaaga ccacgtagtc
     3301 cttgtcgcgc ttgaagagcg ccttcgcctt gatcgcgttg ttcaagaacg agatgagcgg
     3361 cgtgttcgcc gactcgtaca ggttctcgat gcccaggtag tcttcgacct tctcgatgcc
     3421 aggctccagc actccgaccg tgcgcttctt ctcgtcgacc tcgtagtcga cacccacctc
     3481 cagccgggcg gcgaggctcg caaactcggt gaaccaccgg ttcgcctcgc cggaggacgg
     3541 gcccgagatg atgagcggag tgcgcgcctc gtcgatcaag atcgagtcga cctcgtcgac
     3601 gatcgcgaag tagtggccgc gctgcaccat gtcggcggtc tgccaggcca tgttgtcgcg
     3661 cagatagtcg aagccgaact cgttgttcgt gccgtaggtg acgtcggccg cgtactgctc
     3721 gcgacgctct tgcgggttct ggccggagag gatcacgccg gtcgtcatgc cgagggccct
     3781 gaagacccgc cccatgagtt ccgactggta gccggccagg tagtcgttga ccgtgacgat
     3841 atgcacgccg cgggaggtga tcgcgttcag gtacgccggc agggtcgcga cgagggtctt
     3901 gccctcgccg gtcttcatct cggcgatgtt gccgagatgc agcgccgctc cacccatgac
     3961 ttgcacgtcg aacggccgca tgcccagggt gcgtttcgcc gcctcccggg ccgccgcgaa
     4021 cgcctcgggc agaaggtctt ccagcgactc gccggcgccg tgcctctctc gcaggagcgc
     4081 cgtctcgccc cgcagatcct cgtcggagag cgcggcgaag tcgtcttcta gcgccccgac
     4141 agccttggca tagttctgga gtcgacgcaa cgtgcgcccc tcgccgaacc gaaggactct
     4201 ctccaagact gaggccacga atgcactcca tgttgtcgtc gacggcacgc gttagcgctg
     4261 caagaccgaa tctccgccac accggccgca gcgcccaact ataccaaccg acccggtata
     4321 gcctctgagc cgcacagaaa caggcgcccc gatcgagacc cccaaccggc gggggcgggc
     4381 gcctctcggc cacccgcccc cgccgcgtcg caggagccgt gccgtcaggc ggcctcctcc
     4441 cccagcgcga tcaggccgta gtcccagcct ttgcgccgat agacgacgct cggcctgccg
     4501 gtctcggaat cgacaaacag gtagaagtcg tgcccgacca gctccatgcg gtacagggcg
     4561 tcgtcgacgc tcatcggcat cgcctggaag accttgcggc gaatgacgac cgggccgtag
     4621 acgtcgtcgg cgttctcttc ggtcatctcg ccgacgatgg gaatcgtgcc ggtgcgcacc
     4681 ttctcgagaa ggtcgccggg ggcgggcacg atgccggcgc cgccgaacga tttcgaactg
     4741 gcctcgcgca gtgaggtggg tcgtttcatg ccgccacgat gaaccttgcg gcgatctttg
     4801 acacgtcgta tccgctcgac gaggcgggtc tgggcggtgt cgaaggcaga gtacttgtcg
     4861 aagccggtcg cctcgctgcg tacgaccggg cccggcccga tcaacgtgag ctcgacccgg
     4921 tcttcgaggc tcgtcttgtc ggccttctcg cgatgacggc tcaccttcac ctcgagtgcc
     4981 agcgcccgat cggacaggcg ggagatcttt tcagacttgc cggtcacata ctcccgaaag
     5041 cggtcggtga ttccaaggtt gcgtccgaaa atctcgagtt ccatgacgac gacctctccc
     5101 actcatgacg tgggcaccac ccggcgcttt ccggcgggtc accacgcctc ttcccctcaa
     5161 ggtagtgccg gccatgcacc ccggccaccc tccccgaccg gcggcgtgtc acgggcgtcg
     5221 gccacccgcc gaggagtgta tgcgagggcc acgacgccca gcaggccgcc gccggcggcg
     5281 ccgatggccc gggccacctc tcgggtggtc gccccggtcg tcacgatgtc gtcgatgaga
     5341 agccaggcgg agcccgccgg cacccgccgg gcggccaacg aacccgacct gttctcttcg
     5401 cgggcggccc gatcgagggc ggcctgatcc acactctggc gcagcgggcg cagaatctgg
     5461 gccggcttca gccccgcgtg cgatgcgagg cgcggcacgg gccggtaccc tcggcgccgc
     5521 caggccgccc gggtggaggg caccgtcgtg aggcggatac cctgccgcgc cacgacctcg
     5581 cccgcgggga cctgcgtgag cgccgcccgc aatgcggccc gcagcaggcc ggccagagcc
     5641 ggcccgcagt cggtgcgccc gccatctttg taggcgagaa tcactcggcg caaggtgcct
     5701 tcgtagcggc ccgccgccca gaccggcagg ccggcgtcga gatcggcccg cacgacccgg
     5761 ggcgtcatcg cccgcaggca ggaggtgcac agggaacccc cgctcgcccc gcagccggcg
     5821 cacgagacgg gcagcagcag gtcacacgcc tcccgccacc acgtagccat gcttgacagc
     5881 ctggccgacg ggcgccccgt ccacccggcg ttcggaacaa gaggggagaa gcggcccggc
     5941 cggcagcc
//
LOCUS       DASTNL010000197         6556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1626223, whole genome shotgun sequence.
ACCESSION   DASTNL010000197 DASTNL010000000
VERSION     DASTNL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626223"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /gene="uvrB"
                     /locus_tag="VFQ96_06140"
     CDS             <1..1608
                     /gene="uvrB"
                     /locus_tag="VFQ96_06140"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU0257402.1"
                     /translation="EEYLDAMAALQVGEQIGRDRLIRMFVGMQYERNDIDFSRGKFRV
                     RGDTIEIIPMYEENAIRIEMFGDEIEALTTLHPLTGEVIEGLDSVAVFPATHYAAGPE
                     TMRRAIDGIQKELTERLAVFEREGKLLEAQRLKMRTTFDIEMMEQIGFCSGIENYSRH
                     MDGRAPGEPPTCLLDYFPDDFLLVIDESHVTVPQIGAMYEGDASRKRTLVEHGFRLPS
                     ALDNRPLKWDEFRERIGQTVYLSATPGPYEMGRADGVVEQIIRPTGLVDPQIVVKPIR
                     GQVDDLLEQIRVRAARHERVLVTTLTKKMAEDLTTFLAEAGVKVRYLHSDVDTLRRVE
                     LLTELRRGVFDVLVGINLLREGLDLPEVSLVSILDADKEGFLRSTTSLIQTIGRAARN
                     VSGEVHMYADVVTDSMARALDETNRRRAKQIAYNKEHGIDPQPLRKKIADITDVLARE
                     DADTAAMISARHSHSHARAAAGPVGLGPRRLGAEGADKLEELAAQLNSQMLAAAEELK
                     FELAARLRDELADVKKDLRQMEKVGHA"
     gene            1690..4578
                     /gene="uvrA"
                     /locus_tag="VFQ96_06145"
     CDS             1690..4578
                     /gene="uvrA"
                     /locus_tag="VFQ96_06145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0257403.1"
                     /translation="MSVSPAASQPILSVRGARAHNLRNINVDIPRDSLVVFTGLSGSG
                     KSSLAFDTIFAEGQRRYIESLSAYARQFLGQMDRPDVDFIEGLSPAVSIDQKSTNRNP
                     RSTVGTVTEIYDYMRLLWARVGVPHCPVCGAPIGRQSVQQIADRLVALPEGTRYQVVA
                     PVVSQKKGEFVDLFADLAGGGYSRAIVDGEMVQLSDPPTLRKQYKHDILVVVDRLVAR
                     ADILQRLTDSLETALRLADGVVQVNLVDREGEDAWLTFSERLSCPNDHPIALTEIEPR
                     TFSFNAPFGACPECSGLGTRMSVDEDLLLGDPEESIAGGVLIPWSTQGKALFQYYEKL
                     LNGLATELGFDLDTPWRALPRKVQAAVLHGKDFTVKVKWRNRFGRTVSYTSGFEGVVP
                     YIERQYAEAETDNQRQRWAEYLREIPCAVCGGTRLKPEVLAVQVGGASIADVCAMSLT
                     EARDFLAGLSLSEREKRIAVQVLREITVRLDFLVKVGLDYLNLARAAGTLSGGEAQRI
                     RLATQIGSGLTGVLYVLDEPSIGLHQRDNRRLIETLLALRDLGNTLIVVEHDEDTIRA
                     ADWIVDIGPGAGEGGGQVVHSGTYDDLVRNTRSLTGDYLAGRRVIPVPETRRSVDPHK
                     MLTVVGARVNNLKNLTVAFPLGTFIAVTGVSGSGKSSLVDDVLYRVLANKLNGARKLP
                     GKHTRVTGLEHLDKVVHVDQAPIGRTPRSNPATYTGVFDRIRTLFSQTAEAKVRGYRP
                     GRFSFNVKGGRCEACSGDGTVKIEMNFLPDVYVTCEVCGGARYNRDTLQVHYKGKNIA
                     EVLDMPIAEAAAFFEPISAIHRYLKTLVDVGLGYVRLGQSATTLSGGEAQRVKLATEL
                     QRRGTGRTVYVLDEPTTGLHFEDVRKLLEVLGELVDKGNTVIVIEHNLDVIKSADWLI
                     DLGPEGGARGGQVVAEGTPEQVAMVEASHTGRFLAPLVRVPSEQRVSA"
     gene            4575..6491
                     /gene="uvrC"
                     /locus_tag="VFQ96_06150"
     CDS             4575..6491
                     /gene="uvrC"
                     /locus_tag="VFQ96_06150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU0257404.1"
                     /translation="MTDPVAYRPKPGEIPATPGVYRFRDEARRILYVGKAKNLRARLA
                     NYFQPLQNLHERTRTMVTSAASVEWTIVRNEFEALQLEYTWIQEFSPPYNVQFKDDKS
                     YPYLAMTMGDEVPRVFITRNHNIPGARYFGPYTKVWAIRESLDLLLKVFPMRSSSDAV
                     FNRARRTGRPCLLAEIGKCSAPCVGRISVADHRKIAEDLASFLEGNDTGKIRELTESM
                     KKASAEQDFEAAAKYRDEIRALEAVLAKNAIVFSDRVDADVFGIADDELTAAVQQFAV
                     RGGRIRAARGRVVDKELDVALPDLVESVLHRSYQDEEPPREILVPVLPENSADLERWL
                     TERRSLGRVRLRVPMRGEKATLAQTALVNAKNSLALYKTRRSADFVARSQALGDLQEA
                     LGLATAPLRMECYDISHLGGTGVVASMVVFEDGLPRKDQYRKFSIAQTSDDTDSMRQV
                     LERRLAYLAVPAGTGAAEGEEAGPVVAPTLAEEGRRRKFASPPDLVIVDGGLPQVHAA
                     QRVLDAADLPEKVRRRITLVGIAKRLEELWLPGEDFPVILPRSSDALFMIQHIRDEAH
                     RFAITYQRASRRKNIHTTLAGIPGLGPARVKALLKHFGSVRMLRQASLDEMTQVPGIG
                     PQLARAIHRALPDR"
BASE COUNT         1143 a         2253 c         2177 g          983 t
ORIGIN      
        1 gaagagtacc tcgacgcgat ggcggccttg caggtggggg agcagatcgg gcgcgaccgt
       61 ctgatccgta tgttcgtcgg aatgcagtac gagcgcaacg acatcgactt ctctcgcggc
      121 aagttccgcg tgcgcggcga cacgatcgag atcatcccga tgtatgagga gaacgccatc
      181 cgcatcgaga tgttcggcga cgagatcgag gcgctcacga cgctgcatcc gctcaccggc
      241 gaggtcatcg aagggctgga ttccgtcgcc gtgttccccg ccacccacta tgcggccggg
      301 ccggagacga tgcgccgcgc catcgacggc atccagaagg agctcacaga gcgcctggcc
      361 gtgttcgagc gggagggcaa gctgttggag gcgcaacggc tgaagatgcg caccaccttc
      421 gacatcgaga tgatggagca gatcgggttc tgctccggca tcgagaacta ttcccggcac
      481 atggacggcc gcgcgcccgg ggagccgccg acctgcctcc tcgactattt ccccgacgac
      541 ttcctgctcg tcatcgacga atcccacgtc accgtgccgc agatcggcgc gatgtacgag
      601 ggcgacgcct cacgcaaacg cacgctggtc gagcacggct tccggctgcc gagcgcgctc
      661 gacaaccggc cgctgaagtg ggacgagttc cgcgagcgga tcgggcagac cgtgtaccta
      721 tcggcgacgc cggggccgta cgagatgggg cgggccgacg gggtggtcga gcagatcatc
      781 cggcccaccg ggctcgtcga tcctcagatc gtcgtcaagc cgatccgtgg gcaggtcgac
      841 gacctcttgg aacagatccg cgtgcgggcg gcccgtcacg agcgggtcct cgtcacgaca
      901 ttgacgaaga agatggccga ggacctcacc acgttccttg cggaggcggg cgtgaaggtc
      961 cgttacctgc attcggacgt cgacacgttg cgccgcgtcg aactgctcac cgagttgcgg
     1021 cgcggcgtgt tcgacgtgct ggtcggaatc aacctgctgc gtgagggcct cgacctgccg
     1081 gaggtctcac tggtctccat cctcgacgcc gacaaagagg gcttcctgcg ctccacgacc
     1141 tcgctcatcc agacgatcgg ccgcgccgcc cgcaacgtct cgggcgaggt gcacatgtat
     1201 gccgacgtcg tcaccgactc gatggcgcgg gccctcgacg agacgaaccg ccgccgcgcc
     1261 aagcagatcg cctataacaa ggagcacggc atcgaccctc agccgctgcg caagaagatc
     1321 gcagacatca ccgacgtctt ggccagggaa gacgccgaca cggcggcgat gatctccgcc
     1381 cggcattctc attcgcatgc ccgggcggcg gccggcccgg tgggcctggg cccgcgccgg
     1441 ctcggcgccg aaggggccga caagctggag gagctggccg cgcagctgaa cagccagatg
     1501 ctcgccgccg ccgaggagct gaagttcgag ctcgcggcga ggctgcgcga cgagctggcc
     1561 gacgtgaaaa aagacctccg ccagatggag aaggtcggcc acgcctgaag atacgaaaag
     1621 gcgcccgggc gccctggccc gcgggcgaac acgcggcgca gagggctggg cgctgcatag
     1681 actcgtgagg tgtcagtgag cccggccgcg tcccaaccga tcctgagcgt acgcggtgcg
     1741 cgcgcccaca acctgcgcaa catcaacgtc gatattcccc gtgactcgct tgtcgtcttc
     1801 accgggctct ccggctcagg taagtcgtcg ctcgccttcg acacgatctt cgcggaaggc
     1861 cagcgtcgct acatcgaatc gctctcggcc tacgcccgcc agtttctggg gcagatggac
     1921 cggcccgacg tcgacttcat cgagggcctc agccccgcgg tctccatcga ccagaagtcc
     1981 acgaaccgca atccccgttc caccgtcgga accgtcaccg agatctacga ctacatgcgg
     2041 ctcctgtggg cgagggtcgg ggtgccgcac tgccccgtgt gcggggcgcc gatcggcagg
     2101 cagagcgtgc agcagatcgc cgaccggctc gtcgcactgc cggagggcac ccgttaccag
     2161 gtggtcgccc ccgtcgtctc gcagaagaaa ggcgagttcg tcgacctgtt cgccgacctc
     2221 gcggggggcg gctactcccg cgcgatcgtc gacggcgaga tggtgcagct ctccgacccg
     2281 ccgacgctgc gcaaacagta caagcacgac attctcgtgg tcgtcgaccg tctggtcgcg
     2341 cgggccgaca tcctgcaacg cctgaccgac tctctggaga ccgcgctgcg gctggccgac
     2401 ggggtcgtgc aggtgaacct cgtcgaccgg gagggtgagg atgcctggct caccttcagc
     2461 gagcggctct cctgcccgaa cgatcacccg atagcgctca ccgagatcga gccgcgcacc
     2521 ttctccttca atgctccgtt cggtgcatgc ccggaatgct ccggtctcgg cacacggatg
     2581 tccgtcgatg aagacctgct gctcggcgac ccggaggaga gcatcgccgg cggggtgctc
     2641 atcccgtgga gcacgcaagg caaggccctc ttccagtact acgagaagct cctgaacggc
     2701 ctcgccaccg aactaggctt cgacctcgac accccgtggc gtgcactgcc gcgcaaggtg
     2761 caggccgccg tgctccacgg caaagacttc acggtgaagg tgaagtggcg caaccgcttc
     2821 ggccgtaccg tcagctacac ctccggtttc gagggcgtcg tgccctacat cgaacggcag
     2881 tatgcagagg cggaaaccga caaccagcgt cagcgctggg cggagtacct gcgcgagatc
     2941 ccctgtgcgg tgtgtggcgg aacccgcctg aagccggagg tgctggccgt gcaggtcggc
     3001 ggtgccagca tcgccgacgt gtgcgccatg agcctgaccg aggcccgcga ctttctcgcc
     3061 ggcctcagcc tgagcgagcg ggagaagagg atcgcggtcc aggtgctgcg cgagatcacg
     3121 gtgcgcctcg actttctcgt caaagtgggg ctcgactatc tgaacctcgc ccgggcggcc
     3181 ggcacgctct ccggcggcga agcgcagcgc atccgcctcg ccacccagat cggctccggg
     3241 ctgaccggcg tgctgtacgt cttggacgag ccctccatcg ggctgcacca acgcgacaac
     3301 cggcgcctca tcgagacgct gctcgccctg cgcgacctcg gaaacacgct catcgtggtg
     3361 gagcacgacg aagacacgat ccgtgcggcg gactggatcg tcgacatcgg ccccggcgcc
     3421 ggcgagggcg gcgggcaggt ggtgcattcg ggcacctacg acgacctggt gcgcaacacc
     3481 cgctcgctga ccggcgatta cctcgccggc cgccgcgtca tccccgtgcc cgagacccgc
     3541 cggagcgtcg acccgcacaa gatgctgacg gtcgtcgggg cccgcgtcaa caacctgaaa
     3601 aacctgacgg ttgccttccc gctcggcacc ttcatcgcgg tcaccggcgt cagcgggtcg
     3661 ggcaagtcgt cgctcgtcga cgacgtactg taccgggtcc tggccaacaa gctgaacggg
     3721 gcccgcaagc tgcccggcaa acacacccgc gtgacgggct tggagcacct cgacaaggtc
     3781 gtgcacgtcg accaggcacc gatcggccgc acgccccgct ccaacccggc gacctacacg
     3841 ggcgtcttcg accgcatccg caccctcttc agtcagacgg cggaggcgaa ggtgcgcggc
     3901 taccgcccgg gccgcttcag tttcaacgtc aagggcggcc gctgtgaggc gtgcagcggc
     3961 gacggcaccg tcaagatcga gatgaatttc ttgcccgacg tgtacgtgac ctgcgaggta
     4021 tgcggcggcg cccgctacaa ccgcgacacg ttgcaggtgc actacaaggg caagaacatc
     4081 gccgaggtcc tcgacatgcc gatcgcggag gcggccgcct tcttcgagcc gatctccgcc
     4141 atccaccgtt acctgaagac cctcgtcgac gtcggcctgg gctacgtgcg gctcggccag
     4201 tcggccacga cgctctccgg gggagaggcg caacgcgtga agctcgcgac cgagctgcaa
     4261 cgccgcggca cggggcgcac cgtgtacgtg ctcgacgagc cgaccacggg gctgcacttc
     4321 gaagacgtgc gcaaactctt ggaggtcttg ggcgagcttg tcgacaaggg caacaccgtc
     4381 atcgtgatcg agcacaatct cgacgtcatc aaatcggccg actggctgat cgacctgggc
     4441 ccggaaggcg gcgctcgcgg cggccaggtc gttgcggagg gaaccccgga acaggtggcc
     4501 atggtggaag cgagccacac cggccggttc ctggcgcccc tggtgcgggt gccgtccgaa
     4561 cagcgggtga gcgcgtgacg gatccggtcg cctatcggcc gaagccgggt gagatcccgg
     4621 cgacccccgg cgtctaccgg ttccgcgacg aggcccgccg catcctgtac gtcggcaagg
     4681 ccaagaacct gcgggcccgg ttggcgaact acttccagcc gctgcagaac ctgcatgaac
     4741 gcacccgtac gatggtgacg agcgcggcga gcgtcgaatg gacgatcgtg cgcaacgagt
     4801 tcgaggcgct gcaactcgag tacacgtgga ttcaagagtt ctcgccgccc tacaacgtgc
     4861 agttcaaaga cgacaagagc tacccgtacc tcgccatgac catgggcgac gaggtgccga
     4921 gggtcttcat cacccgcaac cacaacattc cgggcgcccg ctacttcggc ccatacacga
     4981 aggtgtgggc catccgggag agtcttgacc tgttgttgaa ggtcttcccg atgcgcagct
     5041 ccagcgacgc cgtcttcaac cgggccaggc gcaccgggcg gccttgcctg ctcgcagaga
     5101 tcggcaaatg ctccgcgccg tgtgtcggcc gcatctcggt cgccgaccac cgcaagatcg
     5161 ccgaagacct cgcctccttc ctcgaaggca acgacacggg caagatccgt gagctgaccg
     5221 agagcatgaa gaaggcgtcg gcggaacaag acttcgaggc cgcggcgaaa taccgtgacg
     5281 agatccgcgc actggaggcg gtgctcgcca agaacgcgat cgtcttctcc gaccgggtcg
     5341 acgccgacgt gttcggcatc gccgacgacg agttgacggc ggccgtccaa cagttcgccg
     5401 tgcggggcgg ccgcatccgg gccgcgcgcg gacgggtcgt cgacaaggaa ctcgacgtgg
     5461 cgctgcccga cctcgtcgaa tcggttctcc atcgctctta ccaagacgag gagccgccgc
     5521 gggagatcct cgtgcccgtg ctgccggaga acagcgccga cctggaacgc tggctcaccg
     5581 aacgccgctc cttaggcagg gtgcggctgc gggtgccgat gcggggcgag aaggcgacgc
     5641 tcgcccagac ggcgctcgtg aacgcgaaga actcgctggc gctgtacaag acccgccgct
     5701 ccgccgactt tgtcgcccgc tcgcaggctc tcggcgacct gcaagaggcg ctgggtctcg
     5761 ccacggcgcc gctgcgcatg gagtgctatg acatctctca cctgggcggc accggcgtgg
     5821 tcgcctcgat ggtggtcttc gaagacggcc tgccgcgcaa agaccagtac cgcaagttct
     5881 cgatcgccca gacgagcgac gacaccgact cgatgcggca ggtcttggag cgccggctcg
     5941 cctacctggc cgtgccggcc ggcacgggtg cggccgaggg ggaggaggcg ggccccgtcg
     6001 tggcgccgac gcttgcggaa gagggccgcc ggcgcaagtt cgcctcgccc cccgacctgg
     6061 tgatcgtcga cggcggcctg ccccaggtgc acgccgcgca gcgggtgctc gacgcggcag
     6121 acctgcccga gaaggtgcgc cgccgcataa ccctcgtcgg catcgcgaaa cgcttggagg
     6181 agctgtggct gccgggggag gacttccccg tcatcttgcc gcgttccagc gacgcgctct
     6241 tcatgatcca gcacatccgc gacgaggccc atcgcttcgc cataacctac caacgggcct
     6301 ccaggcgcaa gaacatccac accacgctgg ccggcattcc cggcttgggc ccggcccggg
     6361 tcaaggcgct tctgaagcat ttcggctccg tccgcatgct ccgccaggcg agcctcgacg
     6421 agatgaccca ggtgcccggc atcggtccgc agctggccag ggcgatccac cgtgccctgc
     6481 cggaccggta ggctggggtg ccgcagcggc acgggacggc tcgggcatcg gtggggagcg
     6541 aacgtggcgg aagacg
//
LOCUS       DASTNL010000198         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_147561, whole genome shotgun sequence.
ACCESSION   DASTNL010000198 DASTNL010000000
VERSION     DASTNL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147561"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFQ96_06155"
     CDS             <1..518
                     /locus_tag="VFQ96_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257405.1"
                     /translation="VAGVLIPDEGTITVDGGVAPLIEITGGFVDDLTVRDNVVLTAGL
                     HGMTRSEVDAAFDDIIDFAEIGAFVDTPYKHLSSGMKVRIAFSVISRLDEPIILVDEA
                     LAVGDKAFREKCYRRIEAMLEGGRTLFFVSHSEGDLRRFCTRGLYLDKGGLKLDADLT
                     DVLDAYNSDYH"
     gene            739..1524
                     /locus_tag="VFQ96_06160"
     CDS             739..1524
                     /locus_tag="VFQ96_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257406.1"
                     /translation="MHIRRKPRPLAASLVTSEGEVRPRRPLPALVESGLDRGIERRRA
                     AVERTLRRVVRGLPPDAGGADVVAAIERRFLMRASLTGAAAGAAAAAPGVGIVAGLAF
                     SAADTAAFFSAGAFFVQSRLQLEVSPEDDPQRLRGLILTLLLGPGGSALLRQLGGELA
                     DDGPSRHEYRTSVVARAYRATGLWLPLLTGGWRGEGPDTGRAARAGGRALAGRLAPYG
                     IGAVLGAASARGTARQVVLAARDLFDEEPAIPDRLRAQPGRRA"
     gene            1542..4544
                     /locus_tag="VFQ96_06165"
     CDS             1542..4544
                     /locus_tag="VFQ96_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU0257407.1"
                     /translation="MPRFTFAAGNLRKILAAPLYVLGEAASLFARRDEKLWVFGSGVG
                     VGEGALALARHAHAADPGLHIVWMARGQADIAQALTAGFDAVRKDSWRGFLCTLHAGV
                     VVVTHGFGDANRYATRGAFTVQLWHGIPFKKIHLDSPETLRLPPWLGGVPGVRAALRR
                     AYAYSGHRIGMFVAASRVAAGRLKTAFALDDRQFAITGDPRDDAVFGAPDEARQSLAR
                     LVGDERARTCRIILYAPTWRDGGDDPSIPGDDEWRRIGRVLEETDSILLLRSHPLGAG
                     HHANTPARVKGLPATLAPDINPFLPAIDVLVTDYSSIAFDFALLGRPIVFFAPDLDAY
                     TRRRGTYEPYACLSGGADVRTWGGLARRLSTLASDAAAAEDAARQAQSLARHLHDWHD
                     GASTARTYDELRRRLAGAAPRHTLTPDAAPDGADAAADGPGAADRVPRRRPTRREPEG
                     EGTPGAKTTGDGALDGTDPARTRPRRAALVIDSVALSAGAQTLRLAGRPARPPHAAPP
                     PSARPADPARMVSPAAGVPEAVCLAGARVRLTGTVTMATGPGQDGRWSAEVPLRLTRW
                     SGPELPPPGGAYRLVATIDGETAPCELAPDIRLPDGLEAGLFRLSWNRAEAALTIGAP
                     LNDDERSSAGQERLAARYRKERRLEDAVFFESFYGQNASCNPRGIDRALAALRPDVTR
                     YWSVADRSVPVPHGAVAVVEGTEAWWHARARARTLVVNDWLRKRYRRRYGQTVLQTWH
                     GTPLKRIALMKRGLGPHARLAALREQSRWDLMLAQNPFSVAMFRKAYAFRRAIWQEGY
                     PRNDILVTGDAALRASVRRRLGVTDETRLVLYAPTWRDDRPGEVNHIHAERFRALLGD
                     GYAVLVRGHSRTLLPGHAVTGAGVSDVTGYPDVSELLLAADIVITDYSSIMFDATVTG
                     KPLFFFVPDLERYRDTLRGFTFDLMEAAPGAVTTTVAELAEAVRHAGTLAAEYAERYA
                     RWRARFNPRDDGHAGRRVVLRMIRAGMV"
     gene            complement(4474..5586)
                     /locus_tag="VFQ96_06170"
     CDS             complement(4474..5586)
                     /locus_tag="VFQ96_06170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0257408.1"
                     /translation="MVDESERRTLPGVSYVMPVLNEVEHVGQAIDSLLAQDYEGPFDV
                     TLAVGDSDDGTDELLARRQAQDERIVVVRNEPGSAPAGLNRAIRASRYPVVVRVDAHS
                     VLPPDYTRIAVETMLRTGADNVGGVMDARGETPFEQAVAAIYGSRLGLGGTRLHRGGV
                     EGPAETVYLGVFRRESLVGAGLYDEGIRRGEDWELNRRIRQRGGLVWFTPRLRVLYRP
                     RSRLSDLAWQFFSTGVWRGELVRRFPTANGLRYWAPPALVLALAAGIVVGLVGALRLG
                     SGTGWLLVGFVPPVVYLAGVVAAAAAGRSSPSRALWFLVALPTVHTCWGVGFFVGVAA
                     LARDLQSYSGRRTVTPFRPGSSAARRAGRRGRRREG"
BASE COUNT          857 a         2161 c         1995 g          802 t
ORIGIN      
        1 tcgtcgccgg cgtgctcatc cccgatgagg gcacgatcac cgtcgacggc ggcgtcgccc
       61 cgctcatcga gatcaccggc gggttcgtcg acgacctcac ggtgcgggac aacgtcgtgc
      121 tgactgccgg gctgcacggc atgacccgca gcgaggtcga cgccgcgttc gacgacatca
      181 tcgacttcgc cgagatcggc gcgttcgtcg acaccccgta caagcacctg tcgagcggca
      241 tgaaggtgcg catcgccttc tccgtcatct cgcggctcga cgagccgatc atcctcgtgg
      301 atgaggcgct cgctgtcggc gacaaggcgt tccgcgagaa gtgctaccga cgcatcgagg
      361 cgatgctgga gggcgggcga accctgttct tcgtgtcaca cagcgagggc gacctgcggc
      421 gcttctgcac ccgcgggctc tacctggaca agggtggcct gaagctcgac gccgacctca
      481 ccgacgtgct cgacgcgtac aactccgact accactgacg agcgcaggcc gcaccctggt
      541 ggggcgaggc cgcatgagcc cctggtaact cctgaagctc tgcccggcca ttcaacgtca
      601 tgtgccggca acgtgcagcc ggggaggcgc aatccctccc ggtcatggcc gtcggcaacc
      661 acagccctgt cgggcgtcgc gcttccttcg aggcccgggc cgggccgcgc gcaaaccgca
      721 ggaccgtaaa ctcagcctgt gcacatccgt cgcaagcctc gcccgctcgc cgcctcgctt
      781 gtcacgtccg agggcgaggt acggccacgc cgacccctgc cggccttggt ggagtcgggt
      841 ctcgaccgag gcatcgaacg gcgccgggcg gcggtcgaac gcaccctccg ccgcgtcgta
      901 cgcgggctgc cgcccgacgc cggcggcgcc gatgtcgtcg cggccatcga gcggcgcttc
      961 ctcatgcggg caagcctcac gggtgcggcg gcgggcgcgg ccgccgccgc acccggcgtc
     1021 ggcatcgtcg cgggccttgc gttctcggct gccgacacgg ccgcgttctt ctctgcaggc
     1081 gccttcttcg tgcaatcccg tctgcaactc gaagtctcgc cggaagacga tccccagcgc
     1141 ttgcgcggcc tcatactcac gcttctcttg ggcccgggtg gcagcgccct gctgcgtcaa
     1201 ctgggcggcg aacttgccga cgacgggccc tcccgccacg agtatcgcac gagcgtcgtg
     1261 gcacgggcct accgcgcgac gggcctgtgg ctgccccttc tcaccggcgg ttggcgcggc
     1321 gagggccccg acacgggccg ggccgcacgc gccggcggcc gggcattggc cgggcgcttg
     1381 gccccgtacg gtatcggcgc cgttctcggt gcggcgtcgg cgcgaggcac ggcacggcag
     1441 gtcgtgctcg cggcacgtga ccttttcgat gaggaacccg ccatccccga ccggctgcgc
     1501 gcgcagcccg gccggcgcgc ctgacgagca cggtgtcgtc gatgcctcgc ttcacgttcg
     1561 ccgccggcaa cctgcgcaag attctcgcgg cgccgctcta tgtcttgggc gaggcggcga
     1621 gcctcttcgc gcggcgagac gagaagctct gggtcttcgg cagcggcgtc ggcgtgggag
     1681 aaggcgccct cgccctcgcc cgtcacgcgc atgcggccga cccgggcctg cacatcgtct
     1741 ggatggcccg cggccaggcc gacatcgccc aggcactgac ggccgggttc gacgcggtgc
     1801 ggaaagactc gtggcgcggg ttcctctgca cactccacgc cggcgtcgtc gtcgtgacgc
     1861 acggcttcgg agacgcgaac cgctacgcga cccgcggcgc gttcaccgtg caactgtggc
     1921 acggcatccc cttcaagaag atccacctcg actcccccga gaccctgcga ctgccgccct
     1981 ggctcggcgg ggtgccgggg gtccgcgcgg cgttgcgccg ggcgtatgcg tattccgggc
     2041 accggatcgg catgttcgtg gcggcctcgc gggtcgcggc cggccggttg aaaaccgcct
     2101 tcgcgctcga cgaccgccag ttcgcgatca ccggagaccc gcgggacgac gccgtgttcg
     2161 gggcaccgga cgaggcccgg cagagcctcg cacgcctcgt cggagacgaa cgggcacgaa
     2221 cgtgccgcat catcctgtac gcaccgacct ggcgtgacgg tggcgacgac ccgtcgattc
     2281 ccggcgacga cgagtggcgc cggatcggcc gcgtcttgga ggagaccgat tcgatcctcc
     2341 tgctccgctc gcaccccttg ggggccggcc accacgcgaa cacgccggcg cgggtgaagg
     2401 ggctgcccgc cacgctcgca cccgacatca acccgttcct gcccgcgatc gacgtgctgg
     2461 tgacggacta ctcctcgatc gcgttcgact tcgccctcct cggtcggccc atcgtctttt
     2521 tcgcgccaga cctcgacgca tacacccgcc ggcggggcac ctacgagccg tatgcgtgcc
     2581 tcagcggggg agccgacgtg cggacctggg gcggcctcgc ccgccgcctc agcaccctgg
     2641 cgtcagatgc ggcggccgcg gaggacgcgg cacggcaagc gcagagcctc gcccggcacc
     2701 tccacgactg gcacgacggc gcgagcaccg cgcgaaccta cgacgagctg cgacgccggc
     2761 tggcaggggc cgcaccccgc cacacgctca cccccgacgc ggcgccggac ggcgccgacg
     2821 cggcagccga tggccccggt gctgccgacc gtgtgccccg ccgtcggccg acacgacgag
     2881 aaccggaggg cgagggcaca cccggcgcca agacaaccgg cgacggggcc ttggacggca
     2941 cggatcctgc gcgcacaagg ccgcgccgcg ccgcactcgt gattgactcg gtggcgctct
     3001 cggccggggc gcagacgctc cggctggccg gccggcccgc gaggccgccc cacgccgccc
     3061 cgcctccctc ggcgcggcct gccgaccctg cgcgcatggt cagcccggcg gcaggcgtgc
     3121 ctgaggcggt ctgcctcgcg ggggcccggg tgcgcctcac cggcaccgtc acgatggcaa
     3181 caggccccgg gcaggacggg cgatggtcgg ccgaggtacc gcttcgcctc acccgctggt
     3241 cgggcccgga actgccgccg ccgggcggcg cctaccgtct cgtcgccacg atcgacggcg
     3301 agaccgcacc gtgcgagctg gcacccgaca ttcggctccc cgacggcctc gaggcaggac
     3361 tgttccggct ctcgtggaac cgcgcggagg ccgccctgac aatcggcgcc cccctgaacg
     3421 acgacgagcg cagcagtgca ggccaggagc gcctggcggc ccgataccgc aaagaacgcc
     3481 ggctggaaga cgcggtcttc ttcgagagct tctacgggca gaacgcgtcg tgcaacccgc
     3541 gcggcatcga ccgggcgttg gcggctttgc ggcccgacgt cacacgctac tggtcggtgg
     3601 ccgatcgttc cgtgccggta ccgcacggcg ccgtcgccgt cgtcgaaggc accgaggcat
     3661 ggtggcatgc ccgggccagg gcccgcacgc tcgtcgtcaa cgactggctg cgcaaacgat
     3721 accgccgaag gtacggccag acggtcttgc agacctggca cggcacaccc ctgaaacgca
     3781 tcgccctcat gaagcgcggc ctcggaccgc acgcccgcct ggcggcgttg cgcgaacaat
     3841 cccggtggga cctgatgctg gcgcagaacc cgttcagcgt ggcgatgttc cgtaaggcgt
     3901 acgcgtttcg ccgagccatc tggcaggagg gctacccccg caacgacatt ctcgtgacgg
     3961 gcgatgccgc cctgcgggcg agcgtgcgcc gcaggctcgg tgtcacggac gagacgcggc
     4021 tcgtcttgta tgcaccgacc tggcgggacg accggccggg cgaggtgaac cacatccacg
     4081 ccgaacgctt ccgcgccctc ctcggcgacg gctacgccgt tctcgtgcgc gggcactccc
     4141 gcaccctcct gccggggcac gccgtgaccg gcgccggggt gagcgacgtc accggctacc
     4201 cggacgtctc ggaactgctg ttggccgccg atatcgtcat caccgactac tcgtcgatca
     4261 tgttcgacgc gaccgtgacc ggcaaaccgt tgttcttctt cgtgcccgac ctcgagcgct
     4321 accgtgacac cctccgcggt ttcacattcg acctcatgga agccgccccc ggtgccgtca
     4381 cgacgaccgt cgccgagctg gcggaggcgg tgcggcacgc cggcaccctc gcagcggaat
     4441 acgccgagcg ctatgcccgt tggcgagcgc gcttcaaccc tcgcgacgac ggccacgccg
     4501 gccggcgcgt cgtgctgcgg atgatccggg ccggaatggt gtgaccgttc gcctgccgga
     4561 ataactctgc aggtcacggg ccagggcggc gacgccgacg aagaacccga cgccccaaca
     4621 ggtgtgcacg gtcggcaggg cgacgaggaa ccagagcgca cggcttggcg acgaacgccc
     4681 cgccgcggcc gcggcgacca cgccggcaag gtagacgacg ggcggcacga aaccgaccaa
     4741 cagccacccg gtgcccgagc cgaggcggag ggcgccgacc agcccgacca cgatgccggc
     4801 ggccagcgcg agaacgaggg cgggcggcgc ccagtagcgc aggccgttcg cggtcgggaa
     4861 acgccgcacg agttcgccgc gccagacgcc ggtggagaag aactgccaag ccaggtctga
     4921 caggcgggac ctcggccggt agagaacccg caaccgaggc gtgaaccaga cgagtccgcc
     4981 cctctggcgg atccgcctgt tcaactccca gtcttcaccc cgccggatgc cctcgtcgta
     5041 gaggcccgcc ccaacgaggc tctcgcggcg gaagaccccg agatagaccg tctcggccgg
     5101 cccttcgacg ccgccgcgat gcaagcgcgt gccgcccagg ccgagccggc tgccatagat
     5161 ggccgcgacg gcctgctcga acggggtttc gccccgggca tccatgactc cccccacgtt
     5221 gtcggcgccg gtacgcagca tcgtctcgac cgcgatacgg gtgtagtcgg gcggcagcac
     5281 cgaatgcgca tccacccgca cgacgaccgg gtagcgcgag gcgcgaatgg cgcggttgag
     5341 gcccgccggg gccgaacccg gctcgttacg cacaacgacg atgcgctcgt cttgcgcctg
     5401 gcgtcgggca agaagctcgt cggtgccgtc gtcgctgtcg ccgacggcga gcgtgacgtc
     5461 gaacgggccc tcatagtctt gcgccagcag gctgtcgatc gcctgaccga cgtgctcgac
     5521 ttcgttcaac acgggcatga cgtaagagac gccgggcagg gtacggcgtt cgctctcgtc
     5581 aaccatgtgc cccattcgcc gttcagacgc ttgagcctat cagccggcaa acaggcggcc
     5641 ggcgcggccc tcgcaagggt tccgcgccga ccgcctggca gaggatgagc cgcgtcagct
     5701 ttgcgacatc gtcccgagcg tgacggtcac ggtatgggta ctgccaccgc gcacgtagga
     5761 gagccgtgcg gtgctgccgc cggcgaggta gcgcacttgt gccgtcaggt cggtc
//
LOCUS       DASTNL010000199         9904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_148417, whole genome shotgun sequence.
ACCESSION   DASTNL010000199 DASTNL010000000
VERSION     DASTNL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148417"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ96_06175"
     CDS             complement(<1..456)
                     /locus_tag="VFQ96_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0257409.1"
                     /translation="MTRHFLRDDDISPAEQTEILDLAVQMKAHRFAYRPLAGPQTVAV
                     LFDKTSTRTRVSFHAGIADLGGSPLVISRGDSQLGGKESVADTARVLERMVAAIVWRT
                     YAQAGLEEMARGTRVPVVNALSDDFHPCQLLADLLTIRERRGTLAGQVVA"
     gene            complement(483..1724)
                     /locus_tag="VFQ96_06180"
     CDS             complement(483..1724)
                     /locus_tag="VFQ96_06180"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEU0257410.1"
                     /translation="MTGTTEWRSRYAAHMMNTFAVPARLLVRGSGTRVWDDRGVEYLD
                     FLAGIAVNVLGHAHPALVAAVSEQVGKLVHVSNYFATKPQIEFAERLLRLAGAGEGPG
                     EVPGRVFFANSGTEANEAALKIARLHRPRGRVLALHQSFHGRTMGSMALTGKPKLRAP
                     FEPMPAGIEHIDSTVEALEEAMGDDVAAVFLEPIKGEAGILDLPEGYLAEARRITREH
                     DALLILDEIQTGVGRTGRWFAFQHDGIMPDVVTLAKGIAGGVPTAATIAFGPAAELLT
                     PGQHGTTFGGNPLATAAGNAVLGEIERAGLVENAARRGAELRALLTGPLAAGEGGDLI
                     VGTRGRGLMIGVALSRPVGHRLYDACLDAGLIVNAPNETTIRLVPPLILDDRDIADFA
                     ERFAAGLAAVKQAAPATAGAR"
     gene            complement(1721..2707)
                     /gene="argB"
                     /locus_tag="VFQ96_06185"
     CDS             complement(1721..2707)
                     /gene="argB"
                     /locus_tag="VFQ96_06185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU0257411.1"
                     /translation="MDTDGVDATVKTQVLIESLPWVRRFYGERIVVKLGGNAMVDPEL
                     QKSFAEDMVYLRYAGVQPVVVHGGGPQISAMLTRLGIPSVFEGGYRVTTPEVMEVVRM
                     VLSGSVARSLVAAINRHGPLAAAVSGEDAGLFAARRRRHDAEGNPVELGRVGDIVSVD
                     PSVVVGHLEAGRIPVVSSISSDTDEAGGSLNVNADAAAGALAVALGAAKLVVLTNVAG
                     LYRDWPNEDSLVGAIGAEELRRMLPHLESGMVPKMTACLAAVDGGVPKAAIIDGRVPH
                     SILLEVFTRRGSGTEVVAGLPADGRGPVPPAAPTHETSWPIDAAQPGKEGEL"
     gene            complement(2776..3924)
                     /gene="argJ"
                     /locus_tag="VFQ96_06190"
     CDS             complement(2776..3924)
                     /gene="argJ"
                     /locus_tag="VFQ96_06190"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU0257412.1"
                     /translation="MSVTAAAGFSAAAVVAGLKESGGLDVALVVNSGPSHAAAAVFTG
                     NRAKANPVLWSERALADHTAWAVVLNAGGANCFTGAAGFETSRATAEAAAAALRTEAS
                     NVVVCSTGMIGDELDREKMVAAVTRAAAALGADGGPASRAILTTDTHPKTAARSADGY
                     TLGAMAKGAGMLAPGLATMLVVITTDADLDGQVLERALHEATRVTFNRLDSDGCMSTN
                     DQVTLLASGASGTSPDPAAFTAELTALCADLARQLQADAEGAAHEIAIEVTGAEHEDD
                     AVEVGRAVARSNLFKTAVFGNDPNWGRVLAAIGTTRARFDPYAVDVTMNGVRVCHAGG
                     PDRPRDEVDLTPRAVRVVIALHAGAATATVLTNDLTHDYVHENSAYSS"
     gene            complement(3921..4979)
                     /gene="argC"
                     /locus_tag="VFQ96_06195"
     CDS             complement(3921..4979)
                     /gene="argC"
                     /locus_tag="VFQ96_06195"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU0257413.1"
                     /translation="MTYSVAVAGASGYAGGEVLRLVAGHPEFEVTTVTAHTHAGEPLV
                     RHQPQLRSLSHLRLQETNRESLAGHDIVFLALPHGASAGISAELDETPLVVDCGADHR
                     LTDPADWERFYGGQMAPPWVYGVPELPLARPVGPTGKQRDALVGARRIASPGCNASAV
                     SLALAPGVRAGIVDEHDIVTVLAVGSSGAGKKTQVPYLASELFGTATPYAVGGGHRHI
                     PEIRQNLTMAGATDPTVSFTPVLVPMARGILVTATARLRPGVEPRAVRRAWEETYAGE
                     PFVHVLPEGVFPRTADVVGANTALIGVTVDEAVGRVVAVLALDNLAKGTAGAAVQCAN
                     IALGLPEETGLSVDGVAP"
     gene            complement(5201..7735)
                     /gene="pheT"
                     /locus_tag="VFQ96_06200"
     CDS             complement(5201..7735)
                     /gene="pheT"
                     /locus_tag="VFQ96_06200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU0257414.1"
                     /translation="MRVPLSWLAEYVDLKPGTTPEEVHAALVSVGLEEETIHRFGVTG
                     PVVVGEVLDFAEEPQKNGKVIRWCRVRVAPEGQKAADGGADVRGIVCGAHNFVAGDRV
                     VVTLPGAILPGPFPISARTTYGHLSDGMMASARELGLGSDHSGIIRLGQLGVDEPVGA
                     DVIGPLGLDDCAVEINVTPDRGYAFSVRGVAREYSHATGATFRDPALLYPAPPPATGR
                     RVSLADETPLRGRPGARVFTTRVVRGVDPERPTPAWMTARLVLAGIRPRSILVDITNY
                     VMLETGQPLHAYDLDRLVGDITVRRAAAGEHLTTLDGAERSLDPEDLVIADDAGAVGL
                     AGVMGGARTEIGPASRNVLVEAANFDPVSIARSARRHKLPSEASKRFERGVDPKVADT
                     AGYRAVCLITRLAGGSEDGTGSFFDDTRPAEAIRLPDGYVSGLIGLDYTDDEIRGALT
                     AIGCRVEGAPNGLAVTAPTWRPDLGAPCDLAEEVARIVGYDRIPSLLPVAPPGHGLTR
                     TQRLYRAASDALAAAGLTEVLSYPFVTGRENALYGSPDDEAPRAVELANPIDSKAPLL
                     RTSLLPGLIGIARRNLSRGFVDLALYESGLVFRPDGDMALGSRTLPPGGALPGAAVLE
                     RLNAGIPAQPRHLAALFLGDIVPAGPHQPYVPAGLADALDAARLIGRVIGARLEVVEG
                     RHHALHPGRTAALLVVRGHTKTPVGYAGELLPSLAREADLPRVVAVLEIDLGLVARAA
                     ADHVVAGQVSGYPAATQDLSLQVDRRIPAGRVRASVVEGSGTLLEEAALVADYRGPAV
                     PDDMKSLTFALRFRAPDRTLTAAEATKAKEAGAAVAARRDGASVRD"
     gene            complement(7735..8775)
                     /gene="pheS"
                     /locus_tag="VFQ96_06205"
     CDS             complement(7735..8775)
                     /gene="pheS"
                     /locus_tag="VFQ96_06205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU0257415.1"
                     /translation="MPEATEITEAVLGHAVDEALAALGQAGDSAALKAARAAHLAEGS
                     PLALLSMAMRRVPAAEKAAKGRLVGAARARVSQAFQERQQELAEAEAAARLREEALDV
                     TAVPPRRAAGARHPLHVLQERIADVFVGMGWEVAEGPEVESEWYNFDALNFDADHPAR
                     AMQDTFFVAPADSHLVLRTHTSPVQIRALLASPPPVYRVAPGRTYRTDELDATHTPVF
                     HQVEGIAVDKGLTMAHLRGTLDHFVKVIFGDEARVRLRPNYFPFTEPSAELDLWHPTF
                     RDGPRWIEWGGCGMVHPNVLRSAGIDPDVYTGFAFGMGVERALMFRNDVPDMHDMIEG
                     DIRFSQQFGMVV"
     gene            complement(8787..9593)
                     /locus_tag="VFQ96_06210"
     CDS             complement(8787..9593)
                     /locus_tag="VFQ96_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0257416.1"
                     /translation="MLDDPHAPAVRAAARLAGGRGRRRTGLFLAEGPQAVREALLHRP
                     DLVERVYATGAALAAHDDLARLVDTARVSLAEASERVVQALADTVTPQGVVAVCRMPA
                     PGIADVFAAGPRLLVALHEVRDPGNAGTIIRAADAAGAGGVFLTALSVDPYNPKAVRA
                     ATGSHFHLPVVTGVALGDVRERAAAAGIQLIAADVKGDDLLVARREGVLERPTLWLFG
                     NEAHGLADEQLAAADRVLRLPIYGRAESLNLATAAAVCLYESALALQGSR"
     gene            complement(9705..>9904)
                     /locus_tag="VFQ96_06215"
     CDS             complement(9705..>9904)
                     /locus_tag="VFQ96_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU0257417.1"
                     /translation="IQRINAASRQNGLTYNRFIQGLNLAGVEVDRRILAELAVNEPAT
                     FSVLVETAKKALPADTVEQAA"
BASE COUNT         1369 a         3440 c         3688 g         1407 t
ORIGIN      
        1 ggcgacgacc tgcccggcga gggtgccgcg cctctcgcgg atggtcagaa gatcggccaa
       61 cagctggcac ggatggaagt cgtcggagag cgcgttcacg accggcacgc gggtgcctcg
      121 tgccatctct tcgaggcccg cctgggcgta ggtgcgccag acgatcgcgg cgaccatgcg
      181 ctccaggacc cgcgcggtgt cggcgacaga ctccttgccg cccagctggc tgtcgccgcg
      241 gctgatgacg agcgggctgc cgcccaggtc ggcgatgccc gcgtggaagg agacgcgggt
      301 gcgggtcgag gtcttgtcga agagcaccgc gaccgtctgc gggccggcga gcgggcggta
      361 ggcgaagcgg tgcgccttca tctgcacggc aaggtcgagg atctcggtct gctcggcggg
      421 agaaatgtcg tcgtctctga ggaagtgccg tgtcatcgtc atcgtctctg cctgcccgcc
      481 cgtcaccggg cacctgccgt ggccggggcc gcctgcttca ccgcggcgag gcctgccgcg
      541 aatcgctcgg cgaagtcggc gatgtcgcga tcgtcgagga tcaggggcgg cacgagccgg
      601 atcgtcgtct cgttgggggc gttgacgatg agcccggcat cgagacaggc gtcgtaaagc
      661 cggtgtccga ccggccgcga cagggcgacc ccgatcatga ggccgcggcc gcgggttccg
      721 acgatcaggt cgccgccctc gcctgccgcc agggggccgg tcaggagggc gcgcagttcg
      781 gcgccgcgac gggccgcatt ctcgacgagg ccggcccgtt cgatctcgcc caagacggcg
      841 ttgccggcgg cggtcgcgag cgggttgccg ccgaaggtgg tgccgtgctg gccgggtgtc
      901 aggagttccg cggccggccc aaacgcgatc gtcgcggcgg tcggcacccc gccggcaatc
      961 cccttagcca acgtcacgac gtcgggcatg atgccgtcgt gttggaaggc gaaccagcgg
     1021 cccgtgcggc cgacgcccgt ctggatctcg tcgaggatca ggagcgcgtc gtgctcgcgg
     1081 gtgatacgcc tggcctcggc tagatagccc tcgggcaggt cgaggatgcc ggcttcgccc
     1141 ttgatcggct cgaggaagac ggcggcgacg tcgtcgccca tcgcctcttc cagcgcctcg
     1201 acggtggagt cgatgtgctc gatgccggcg ggcatcggct cgaacggcgc acgcagcttg
     1261 ggcttgccgg tcaacgccat ggagcccatg gtgcgcccgt ggaacgactg gtgcagggcc
     1321 aggacacgac cgcgcggccg gtgcagccgg gcgatcttca gcgcggcctc gttggcctcg
     1381 gtgccggaat tcgcgaagaa gacccggccg ggcacctcgc ccggaccctc gccggcgccg
     1441 gccagccgga gcagacgctc ggcgaactcg atttgcggct tcgtcgcgaa gtagttggag
     1501 acgtggacga gcttgcccac ctgctcagag acggccgcga cgagcgccgg atgcgcgtgg
     1561 cccagcacgt tgacggcgat gccggccaag aagtcgaggt attcgacgcc gcggtcgtcc
     1621 cacacccggg tgccggagcc cctgacgagg agccgtgccg gcaccgcgaa ggtgttcatc
     1681 atgtgcgccg cgtagcggga gcgccattcg gtcgtgccgg tcatagttct ccttcttttc
     1741 cgggctgggc cgcgtcgatg ggccaggacg tctcgtgtgt cggagccgcc gggggaaccg
     1801 ggccgcggcc gtctgcgggc aggcccgcga cgacctccgt gccactgccg cgccgggtga
     1861 acacctcgag caagatcgaa tgcggcacgc gcccgtcgat gatggctgcc ttcggcacgc
     1921 ccccgtcgac cgccgcaagg cacgccgtca tcttcggcac catgccggac tcgaggtgag
     1981 gcagcatccg ccgcagctcc tcggcgccga tcgccccgac cagggaatcc tcgttcggcc
     2041 agtcgcgata aagccccgca acgttcgtca gcaccacgag cttcgccgcg ccgagggcga
     2101 cggcgagcgc gccggccgcc gcgtcggcgt tcacgttgag gctgccgccg gcctcgtcgg
     2161 tgtcggagga gatcgaggag acgacgggta tgcgcccggc ctcgaggtgg ccgacgacca
     2221 ccgaagggtc gaccgagacg atgtcgccga cccggccgag ctccacgggg ttgccctccg
     2281 cgtcgtgccg ccggcgacgg gcggcgaaga gtcccgcgtc ctcgccggag acggcggcgg
     2341 caagcgggcc gtgccggttg atggcggcga ccagcgacct cgcgacgctg ccggaaagca
     2401 ccatccgcac gacctccatc acctcgggcg tcgtcacccg gtagccgccc tcgaacacgc
     2461 tcgggatgcc cagccgggtg agcatcgcgg agatctgcgg gccgccgccg tgcaccacga
     2521 ccggctggac gccggcgtag cgcaggtaga ccatgtcttc ggcgaagctc ttctgcagtt
     2581 ccggatcgac catggcgttg ccgccgagct tcacgacgat ccgctcgccg tagaatcgcc
     2641 tgacccacgg cagcgactcg atcaagacct gggtcttcac cgtcgcgtcg acgccgtcgg
     2701 tgtcgatctg ccggcgggat tcacctctcg cccgaccctc gtcttctgcg acgcgtgccg
     2761 caggccctgt ctcactcaac tcgagtacgc gctgttctcg tggacgtagt cgtgcgtcag
     2821 gtcgttggtg agcacggtgg cggtggcggc gccggcgtgc agcgctatca cgacccgcac
     2881 cgcccggggg gtcaggtcga cctcgtcacg cggccggtcg ggcccgccgg cgtggcatac
     2941 ccgcaccccg ttcatcgtca cgtcgaccgc gtaggggtcg aaccgagcgc gcgtcgtgcc
     3001 gatggcggcc aagacccggc cccagttcgg gtcgttgccg aacaccgcgg tcttgaagag
     3061 gttgctgcgg gcgacggccc ggccgacctc gacggcgtcg tcttcgtgct cggcgcccgt
     3121 cacctcgatc gcgatctcgt gggcggcccc ctccgcgtcg gcctgcagct ggcgcgcgag
     3181 gtcggcgcac agggcggtca gctcggccgt gaacgcggcg ggatcggggc tggttccgga
     3241 ggcgcccgag gcgagcagcg tcacctgatc gttcgtcgac atgcagccgt cggagtcgag
     3301 acggttgaag gtcacccgcg tcgcctcgtg gagcgcacgt tcgagcacct gcccgtcgag
     3361 gtcggcgtcg gtcgtgatga cgacgagcat ggtggcgagc cccggcgcca gcatgccggc
     3421 ccccttcgcc atcgcgccga gggtgtaccc gtcggccgaa cgtgccgcag tcttcgggtg
     3481 cgtgtcggtg gtcaggatcg cgcgggaggc gggcccgccg tcggcgccga gggccgccgc
     3541 cgcccgggtc acggcggcga ccatcttctc tcggtcgagc tcgtcgccga tcatgccggt
     3601 cgagcagacg accacgttcg acgcctcggt tcgaagggcc gcggccgcgg cctcggccgt
     3661 ggcacggctg gtctcgaagc ctgccgcccc agtgaagcag ttcgcaccgc cggcattcaa
     3721 cacgacggcc cacgccgtgt ggtctgcgag ggcccgttcg gaccagagga ccgggttcgc
     3781 tttggcgcga ttgccggtga acacggcggc cgcggcgtgc gaggggcccg agttcacgac
     3841 gagtgcgacg tcgaggccgc ccgactcttt caggccggcc acgacggccg ccgcggagaa
     3901 tcctgctgct gcggtcacgc tcacggcgcc actccatcca cgctcaatcc ggtctcttcg
     3961 ggcagcccca aggcgatgtt ggcgcattgc acggccgcgc cggcggtgcc cttggccagg
     4021 ttgtcgaggg cgagcacggc gacgacacgc ccgacggcct cgtcgacggt cacgccgata
     4081 agcgccgtgt tggcacccac cacgtcggcg gtgcggggga agaccccctc cggcagcacg
     4141 tgcacgaagg gctctcccgc ataggtctct tcccacgccc ggcgcaccgc acggggttcg
     4201 acgccgggcc ggagccgggc ggtcgcggtg acgaggatgc cgcgcgccat gggcacgagc
     4261 accggggtga acgacacggt cgggtcggtg gcgcccgcca tcgtgaggtt ctgacggatc
     4321 tcggggatgt gccggtgccc gccgcccacc gcgtacggcg tcgccgtgcc gaagagctcg
     4381 ctggcaaggt agggcacctg ggtcttcttg ccggcgccgg aggaaccgac ggcgagcacg
     4441 gtgacgatgt cgtgctcgtc gacgatgccg gcacggacgc ccggcgcgag ggccagcgac
     4501 accgcgctgg cgttgcagcc gggcgaggcg atgcggcggg cgccgacgag ggcgtcgcgc
     4561 tgcttgccgg tcggcccgac gggccgggcg agcggcagtt cgggcacccc gtacacccat
     4621 ggcggcgcca tctgcccgcc gtagaaccgt tcccaatcgg ctgggtcggt gagccgatga
     4681 tcagccccac agtcgacgac caacggcgtc tcgtcgagct ctgcggagat cccggcggag
     4741 gcgccgtgcg gcaacgccag aaacacgatg tcgtgcccgg cgaggctctc ccggttcgtc
     4801 tcttgcagac gcagatggga gagcgagcgc agttgcggct ggtggcgcac gagcggctcc
     4861 cccgcgtgcg tatgcgccgt caccgtggtg acttcgaact ccggatggcc ggccacgagg
     4921 cgcagcacct caccgcccgc gtagccgctc gcgcccgcga cggcgaccga atacgtcatg
     4981 gctctccttc acactgcttg gccttcgtgg cgcgcgttcg cgcggcgcgg attccgggcg
     5041 gcgacacccc ggcaccggga agggcacaga cgaggcgtcg cctagattcg gaccgcctgg
     5101 cgtcgccgca gggcgcgggc cagcgcccgc acggagaatg cctgcggtgt cggggccatg
     5161 ggccgagtct agcgccccgg cgccgggccg ggccaatcgg tcagtctcgc acactcgcgc
     5221 cgtcgcgtcg ggccgccacg gccgcgccgg cctctttcgc cttcgtcgcc tccgccgcgg
     5281 tcaaggtgcg gtcgggggcg cggaaacgca gcgcgaaggt gaggctcttc atgtcgtcgg
     5341 gaaccgccgg accccgatag tcggccacca aggcggcctc ttcgagaagg gtgccggagc
     5401 cttcgacgac cgacgcgcgc acccggccgg ccggtatccg ccgatcgacc tgcagggaaa
     5461 ggtcttgcgt cgccgcggga tacccgctga cctggccggc cacgacatgg tcggcggccg
     5521 cgcgggcgac gaggcccagg tcgatctcca agaccgcgac gacgcgcggc aggtcggcct
     5581 cccgggcaag gctgggaagc agctctcccg cgtagccgac gggcgtcttc gtgtggcccc
     5641 gaaccaccag gagtgccgcc gtgcggccgg gatgcagggc gtggtgccgg ccctcgacga
     5701 cctcgagtct cgcccctatg acgcggccaa tgagccgggc cgcgtcgagc gcgtcggcga
     5761 ggcccgccgg cacgtacggc tggtgggggc ccgccggcac gatgtcgccg aggaagagcg
     5821 ccgcgaggtg ccgcggctgc gcggggatgc cggcgttcaa gcgttcgagc accgcggcgc
     5881 cgggcagcgc accgcccggc ggcagcgtgc ggctgccgag ggccatgtcg ccgtcgggtc
     5941 ggaacacgag gccgctctcg tagagggcca ggtcgacgaa accgcgggag aggttgcgtc
     6001 gggcgatgcc gatcagcccc ggcaggaggc tggtgcgcaa gagcggcgcc ttgctgtcga
     6061 tcgggttggc gagctccacg gcgcgcgggg cctcatcgtc gggggaaccg tagagcgcgt
     6121 tctcgcggcc cgtcacgaac gggtaggaga gcacctcggt caggccggcg gcggcgagag
     6181 cgtcggaggc ggcgcggtag agccgctggg tgcgggtcag gccgtggccg gggggcgcga
     6241 cgggtagcag ggaggggatg cggtcgtagc cgacgatgcg cgccacctct tccgccaggt
     6301 cgcatggcgc gccgaggtcg ggccgccacg tcggcgcggt cacggcgagg ccgttcgggg
     6361 ccccctcgac ccggcagccg atcgcggtga gggcgccgcg gatctcgtcg tccgtgtagt
     6421 cgaggcctat gaggccggag acgtagccgt cgggcaggcg gatcgcctcc gccggccggg
     6481 tgtcgtcgaa gaacgacccg gtgccgtctt cgcttccgcc cgccagccgg gtgatgaggc
     6541 acaccgcccg gtacccggcg gtatccgcga ccttcgggtc gacgccgcgc tcgaagcgtt
     6601 tcgacgcctc gctcggcagc ttgtgccggc gggcgctgcg ggcgatcgac acggggtcga
     6661 agttggcagc ctcgacgagc acgtttcggc tagccgggcc gatctcggtg cgggcgccgc
     6721 ccatgacccc ggcgaggccg acggcacccg cgtcatcggc gatgacgaga tcttcggggt
     6781 cgagcgaccg ttcggcgccg tccaacgtcg tcaagtgctc gccggccgcg gcgcgacgca
     6841 ccgtgatgtc gccgacgaga cggtcaaggt cgtaggcgtg cagcggctgg ccggtctcca
     6901 gcatcacgta gttggtgatg tcgacgagga tcgagcgcgg gcggatgccc gccaacacga
     6961 gtcgcgccgt catccaggca ggcgtgggcc tttcggggtc gacgccgcgc accacgcggg
     7021 tggtgaagac ccgggccccg ggccggccgc gcaggggcgt ctcgtcggca aggctcaccc
     7081 gccgcccggt cgcgggcggc ggcgcggggt aaaggagggc ggggtcgcgg aacgtggcgc
     7141 cggtggcgtg cgaatactcc cgggcgaccc cgcgcaccga gaacgcgtag ccccggtcgg
     7201 gggtgacgtt gatctcgacg gcgcagtcgt cgaggccgag cgggccgatc acgtcggcgc
     7261 cgacgggctc gtcgacgccg agctgcccca accggatgat gccgctgtgg tcggagccga
     7321 ggccgagttc ccgggcggag gccatcatgc cgtcggagag gtggccgtag gtcgtgcggg
     7381 cggagatggg gaacgggccg ggcaggatcg cgcccggcaa ggtcacgacg acacggtcgc
     7441 cggcgacgaa gttgtgcgcg ccgcagacga tgccgcgcac gtcggcaccg ccgtcggccg
     7501 ccttctggcc ctcgggcgcg acccgcacac ggcaccagcg gatcaccttg ccgttcttct
     7561 gcggctcctc ggcgaagtcc agcacctcgc cgacgacgac cgggccggtg acgccgaagc
     7621 ggtggatggt ctcttcttcg agacccaccg agacgagcgc ggcatgcacc tcctccggcg
     7681 tcgttccggg cttcagatcg acgtattcgg cgagccagct gagtgggacg cgcatcacac
     7741 caccatcccg aattgctggc tgaaccggat gtcgccctcg atcatgtcgt gcatgtcggg
     7801 cacatcgttg cggaacatga gggcgcgttc gacgcccatg ccgaaggcga agccggtgta
     7861 cacgtcgggg tcgatgcctg cggaacgcag cacgttcggg tgaaccatgc cgcagccgcc
     7921 ccattcgatc caccgcgggc cgtcgcggaa cgtagggtgc cacaggtcga gctcggcgct
     7981 cggctcggtg aacggaaagt agttcggcct caagcgcacc ctcgcctcgt cgccgaagat
     8041 caccttgacg aagtggtcga gcgtgccgcg caggtgcgcc atcgtgaggc ccttgtcgac
     8101 ggcgatcccc tccacctggt ggaagaccgg cgtgtgtgtg gcgtcgagtt cgtcggtgcg
     8161 gtatgtgcgg cccggcgcca cccggtagac gggcggcggg gaggcgagga gcgcgcggat
     8221 ctgcaccgga gaggtgtggg tgcgcaagac gagatgcgag tcggcaggtg cgacgaagaa
     8281 cgtgtcttgc atggcgcggg ccggatggtc ggcgtcgaag ttcaaggcgt cgaagttgta
     8341 ccactcgctt tccacctcgg ggccttcggc gacctcccag cccatgccga cgaagacgtc
     8401 ggcgatacgt tcttgcagaa cgtgcagcgg gtggcgcgct cccgcggcgc ggcgcggcgg
     8461 caccgcggtg acgtcgagcg cctcctcacg aagcctcgct gccgcctcgg cctctgcaag
     8521 ctcctgctgg cgctcctgga acgcctggga cacccgggct cgcgccgcac ccacgagcct
     8581 gcccttcgcc gccttctcgg cggccggcac ccgacgcatc gccatgctga ggagcgcgag
     8641 aggcgatccc tcggccagat gcgcggcccg ggcggccttg agggcggccg agtcacccgc
     8701 ctggccgagc gccgccagcg cttcatcgac ggcatggccg agcacggctt cggttatttc
     8761 ggttgcttcg ggcacggtcc tcaagtttag cgggagcctt gcagggccag agcgctctcg
     8821 tagaggcaga cggccgccgc cgtggcgagg ttcagggatt ccgcccgacc gtatatcggc
     8881 agccgcaaca cccggtccgc ggccgcgagt tgctcgtcgg ccaagccgtg cgcctcattg
     8941 ccgaagagcc acagggtggg ccgctcgagg acaccctcgc ggcgtgcgac caggaggtcg
     9001 tcgcctttga catcggccgc gatcagctgg attccggcag cggcagcccg ctcccgcacg
     9061 tcgcccaagg ccacgcccgt cacgaccggc aggtgaaaat gggaccctgt ggcggcgcgc
     9121 accgccttcg gattgtaggg gtcgacgctg agggcggtga ggaagacgcc gcccgcaccc
     9181 gccgcatccg cggcccggat gatggtgccg gcattgccgg ggtcgcggac ctcgtgcaag
     9241 gcgacgagga ggcggggtcc cgcggcgaag acgtcggcga taccgggggc cggcattcgg
     9301 cacacggcga cgacaccctg cggcgtgacg gtgtcggcca gtgcctgaac cacccgctcc
     9361 gacgcctccg cgaggcttac tcgcgcggtg tcaacgagcc gggcgaggtc gtcgtgcgcg
     9421 gccagtgcgg ctcccgtcgc gtagacccgt tcgacgaggt cggggcggtg cagaagagcc
     9481 tcccgcaccg cctgcgggcc ttcggcgagg aagagccccg tgcggcggcg gccgcggccg
     9541 cccgccagtc gtgcggccgc gcgcacggcc ggggcgtgcg gatcgtcaag catcggccgg
     9601 cggcttcgcg ggcacgacga cgcacgccgg cccccgatgg agggtcggcg tgcgcgcggg
     9661 tcggcgcttt ggcgccccgg gcctcgtcgt gagaccggcg ggtctcaggc tgcctgctcg
     9721 accgtgtcgg ccggcagcgc cttcttggcc gtctcgacga gcaccgagaa ggtcgcgggc
     9781 tcgttcacgg ccaattccgc caggatgcga cggtccacct cgacaccggc caggttcagg
     9841 ccctggatga accggttgta ggtgaggccg ttctgacggc tcgctgcgtt gatgcgctgg
     9901 atcc
//
LOCUS       DASTNL010000200        15317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_703914, whole genome shotgun sequence.
ACCESSION   DASTNL010000200 DASTNL010000000
VERSION     DASTNL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703914"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFQ96_06220"
     CDS             <1..966
                     /locus_tag="VFQ96_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEU0257418.1"
                     /translation="VDGGGTKTAFALARADGALVGTAEGAGSDYVSHGFDAVRQTLRV
                     GLEECLGQAGIGVGDLAGAVFGLPCHGENPADTARLDRLGADLVGGRRSLCVNDMVCG
                     WAGSLGLADGVNVVAGTGSMAYGRRAGSEARCGGWGEIVGDEGSAFWIGRRLLALFSH
                     MSDGRTPRGALYEIVRSRVVVGDTDLEVVPRFVAGAPELRRRVAALAPAAAEAALAGD
                     EGAADILRVAGRELARLARASAMALGYSAGEAIRVSYSGGVFGAGPVLGSFRSALTNA
                     GSFTVTAPVTSPVFGALAYAAREFGEGIDLAALARADGRRRGVPG"
     gene            1105..1941
                     /locus_tag="VFQ96_06225"
     CDS             1105..1941
                     /locus_tag="VFQ96_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0257419.1"
                     /translation="MWRRVAALAPDVAAMLPQPGERVAVVGCGTSWFMAMSYASARES
                     AGLGLTDAFAGSEYPASRTYDRVVAISRSGTTSEIIALLEGLGGRESTVVTGVADSPV
                     TTLATHTVALPFADERSVVQTRFATSTLALLRSGLGDDVASLADQADEALRVDVEPLL
                     DAEQVAFIGRGLAVGLSFEAALKTREAAQFWAESYPAMDYRHGPIAIAQPGRLTWSLG
                     PAPEGLEAQVGETGARFVHHDLDPMAGLVVAQRFAIGVALKRGLDPDHPRHLTRSVIL
                     TS"
     gene            1938..2918
                     /locus_tag="VFQ96_06230"
     CDS             1938..2918
                     /locus_tag="VFQ96_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0257420.1"
                     /translation="MTGRGLLGVDVGGTGIKALLVDAAGAWAGRWRRPTPHGDTGGER
                     TAQTVAELVSQARSVATGRGFDVAAVGLVVPGVVEAARGVCRLAVNLGWKDLPIRQMV
                     AKRVGLPVAFGHDVRAGALAEARTGAAAGLRGLAMFLPVGTGISAAYTRGGEPFELGP
                     AAGEVGQIVLRRGPGAGLRVEEVASAAGIARRVGRPNALAVAEAVRAGDPAAVAAWRE
                     AVEVLADVVAWSAATLDPEVIVVGGGLSAAGDLLFRPLRDAVSERVDGIVVPSLRAAV
                     HGDRAAMVGAILLAGDLVGAGPRGVGRHGAAEGVGADVPTGGTEDAGEAR"
     gene            2915..3841
                     /locus_tag="VFQ96_06235"
     CDS             2915..3841
                     /locus_tag="VFQ96_06235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEU0257421.1"
                     /translation="MSGHVVIVTANPAVDVTYGLAVNKPGETNRVQRVVRRAGGKGLN
                     VAHVLTALGVEARSVLPAGGDAGSWLRAELSRDPAVGAIDVVPIGGLTRQTVAIVADA
                     GHPTLYVEPGPRLTAEEWDRVADAVAKACEGAAFLVVSGSLPAGAPGDLVGRWVRAAR
                     ARGARSLVDCSGPALVASAEAEADIIKPNEAELLAATGAATLEAGVAQLRRCGAGRLV
                     VSRGAEGLLVFDGEARALVPAVPGVTGNPTGAGDAAAAGLVAALVRGYPLEAAARWAG
                     AAGAAAVRQPVAGVIDTADFDAYVEALGGPRR"
     gene            4005..4937
                     /locus_tag="VFQ96_06240"
     CDS             4005..4937
                     /locus_tag="VFQ96_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU0257422.1"
                     /translation="MTPNPGEAQGDLAVPAAATAGMGLPGEVGTRAEALPPRPVTELL
                     RALAADRHGVGAFNVVLLEHAEAIVAGAAEAGLPTLLQISENCVRYHGALRPIVASCR
                     ELAASCPVPVFVHLDHITDRRLVEEGLAAGVDSVMFDGSKLPYDENVAATAAVVRACH
                     ARGVSVEAEIGEVGGKDGVHAPGVRTRPGEAAQFALATGVDALAVAVGTSHAMTSRTA
                     SVDHDLISRIAAAVAVPLVLHGSSGVPDGDLAKAVAAGMTKVNISTHLNKLFTGEIRR
                     VLGPDPDVVDPRKYVAPARTVVAAEVARLLRLLA"
     gene            5094..6323
                     /locus_tag="VFQ96_06245"
     CDS             5094..6323
                     /locus_tag="VFQ96_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257423.1"
                     /translation="MSEILHMPDHAGGTSHRPTPGAPPRVHRASGWRVAAMVASHLFD
                     DLYQGAVPAMLPFFVAERHYGYVAAAGITLAATLLSSVVQPLFGLLTDRHPMPWLVPA
                     GLSVAGLGIALSGLVDSYLWTWCAIALSGVGVASYHPESTRLARAASDGSHVGMSWYS
                     LGGNVGFALGPIVVAPVVSAFGLPATPLLIIPAVVAGVALALLLRPLASKAQVFAAGA
                     LAGLEDDWRQFSLLAVIVTGRSIVGFALNTFLALWVIEHLHAHGQFEGQVALVLYFGC
                     GAVGTLAGGFLARRWERVRILRLSYLLSVPALAGIGLAPGAFVFFFIAASGIVLFLPF
                     SLQVTLGQDYLPTRIGTASGVTLGLSISIGGLAAPAIGALAEAIGLEGAILVLIVVPA
                     ACWLLSLWIREPRQVTG"
     gene            6462..7904
                     /locus_tag="VFQ96_06250"
     CDS             6462..7904
                     /locus_tag="VFQ96_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0257424.1"
                     /translation="MNAVDAVGEPRASRALFPSIGVVTIVVADEFETRRVMTMLAEKS
                     CLQEVRVVVGLEALPQEGPTLVVGPLTRQLDYDDLSRPGLDFLPEPVDAEALVDRVRV
                     CAVRGLAEKEPYGPADYGRLVQEIADSLNVAVLFFDTAGHPRLRNRMVREVLALAGYD
                     PATGLAEQVYAADRHTPVKRGRNILTEALAGHGRGVVYWVGHPSEAERQRAIITEAHT
                     IQRPDGQALGTAMLVYDVTETVLLTEEQADYVSALSHELRTPLTAAMGYLDLIAEDHD
                     IERLGIADEFTIAQRSMSQLAGLLERFSSVGLRRGALVLTPTDLAALAGQAAHAVRPI
                     ADRAGVALHTRLPDTSLLGRADPLRLRQVFDNVLSNAVKYTPEGGSVTFALDREGDEA
                     VITVTDTGIGIDPADQHRVFDRFYRTPSLRGGSIGGHGIGLAIARTVTQAHGGTIRVR
                     STPGLGSTFTIRLPLRPKGSTLPQIHDETE"
     gene            8310..9077
                     /locus_tag="VFQ96_06255"
     CDS             8310..9077
                     /locus_tag="VFQ96_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HEU0257425.1"
                     /translation="MGGTPGRVTRLAAAMVAGLALALAGPLSASAHVRVSPDTATPGG
                     YALLTFRVPTESATASTVRLEVDLPKDTPFSSVSYEPVAGWATSVTTSTLATPVKTKN
                     ATITEAATKVVWTATSKAARIAPGQFRQFTISAGPVPETGKVVLPAHQTYSDGTVVDW
                     AEPTPASGVEPQHPAPTVYITDAPPGDVSSAAGAPTVTATPRADGASALDVAAGRNTT
                     VDAVALGSGIGGLALGAIALVVALAGLAHRRAGTGRP"
     gene            9152..9910
                     /locus_tag="VFQ96_06260"
     CDS             9152..9910
                     /locus_tag="VFQ96_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEU0257426.1"
                     /translation="MLALVAGVFLVLGGADAASAHDYPVRWSPAAGSTVTTPPTTVSI
                     TFDDLVLDLSGDSHTSLLQVTHTSQGATRYFATGCTVTKGREVSVPVVLGPAGDYKVS
                     YQIVSADGHVVSGNSTFHYRPASGTTQAAGTSSPACEAAGPARAASPSATAGRSGGGV
                     SGTSGSAGLPFGIVLGVAGTIVALAVIAVVVVLFTTRRARPASRTSRWRPQDGEARGA
                     DGTPHGEATGGGPGGGGREASGGEADGSGADPEE"
     gene            complement(9957..10685)
                     /locus_tag="VFQ96_06265"
     CDS             complement(9957..10685)
                     /locus_tag="VFQ96_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257427.1"
                     /translation="MNEHTSEALNVTGLFAGYGRFDVVHDVALNVAKGHAVALLGPNG
                     AGKTTVLRAIMGMLKHRRGTIVVDGVDVSAMPTHKVARGHVALVPEGRRLFLGQSVED
                     NLRLGGLHLHRDKERTEELLQSVFDLFPVVERYRSRPATAMSGGEQQMVAIGRMMMSA
                     PSLMLLDEPSLGLAPLAIQDMANALIALRERGQSILLVEQRVDLALQVCDHVYVLSGG
                     RIVRQSAASEVDSQGRELIDAYLG"
     gene            complement(10682..12526)
                     /locus_tag="VFQ96_06270"
     CDS             complement(10682..12526)
                     /locus_tag="VFQ96_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257428.1"
                     /translation="MSRRQTRQGAPAPSGRSRLMRVTTLAITLAYLLSISGVAHGNFF
                     AQSLFVTGGVYAVLAVSLDLVAGMTGLYSLGHAGLFALGAYGTTLLYHAGWNMFVLLP
                     VVIVGVGIIGAVLGTLSLRVSGLYFAIMTFIFSLVVTVVLTDFSFTGGLNGIAGPSLP
                     DFPQSVSFLGSSLVWCVGLVLLAAVVVVWSIRASAMYPVLLAIRDAEPFAAANGVRTA
                     ATKVGVFALSAGLAAMAGWAFSFTGYISPGQFTWTMSVNILVMVIFGGINTRVGPLIG
                     AAFVSAFPVVVAIDPLVQEAIFGLIFLLVIVLFPGGVMGLAGWLWRRFVRRRPEKTPA
                     VGDARAGQGRLDTAGPSVTNTPPPVGHALECQGIQFAYIKGNTVVDGVDLRVRRGTIH
                     GLIGPNGSGKSTLVNLISGALQPESGTITIGGRRVDGLPAYRRPRFGLLRTFQTAVLA
                     DELSIRESVTIGLYHRIPRIAVRSMIWPLLPTALRDHRRMRAEAGAAISDAGLAASWN
                     SVRVADVPHGVEQLTQLAAACVGGPDILILDEPLAGLSPSEVAQVTDIVGNLRSRGVT
                     MVIVEHQTQFIFDVCDDVTVLAAGKLVASGPADEVRQNERVREVYLGQ"
     gene            complement(12535..13452)
                     /locus_tag="VFQ96_06275"
     CDS             complement(12535..13452)
                     /locus_tag="VFQ96_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0257429.1"
                     /translation="MLQVLLSGLATGAIYGLVAMAFAVVFYVTKVINFATGQLLMVSI
                     MAVSGLVAIGLPTLIAIVVGLILSTGFGVAIYYGAVAPVLKVDRTGFGWLVSTLGVAI
                     VLASVAAMIFGPTSRAFPPLFYHEKIRFGGASTTWQEMLAVIVAAVVLVAFELFRTRT
                     LFGKVGMAISGDPEMASAVGANATGYAVVAFAIGGLLAGIAGVLVGPITFANPYLGDT
                     YGIAGFIALMIGGIDKPAAAMGGGVLLGILSVAANTYINSQASDWFPFVVVVVVLLIT
                     PHGLFSSGNLFTRLSRSLRARRASVGVQS"
     gene            complement(13547..14731)
                     /locus_tag="VFQ96_06280"
     CDS             complement(13547..14731)
                     /locus_tag="VFQ96_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0257430.1"
                     /translation="MKKIALASIAALAALALAGCSGGGGSTGSSSGGGGGTILVGGIA
                     GTTGAYGTTGQAAFNGAELAIDEINSAGTVLGKKLKFQGYDDGADATKASQLFSKLQS
                     QGAVAILGSPDQGPTVAALADQKKFPVLGPVDDGGLSVYPKGPDSDPYAWAWSTSLNT
                     FAWGQKLGEYALKNCTGLAVLHDPSGYGMGGLAGIQLAYKAANKKLALTETITENWST
                     GATVGLKSEISKIKKSGADCVDVWLTPQDQASFMQEAETEGANFTFLGNDETCSDTTF
                     SGLAKKQGDGMICALITTDMNPNARLTKFRADYKKKFNVDATAFAELTYDAVYILKKA
                     IEEGKSTQPESLRTQLNKITDFDGLTGSLTFTEKQHTTLTPEQLTVVKYDAASDAWVP
                     VS"
BASE COUNT         2144 a         5219 c         5524 g         2430 t
ORIGIN      
        1 gtcgacggcg ggggtacgaa gaccgcgttc gccctcgcga gggctgacgg cgccttggtg
       61 ggcacggcgg agggcgcggg gagcgactac gtcagccacg gcttcgacgc ggtgcggcag
      121 acgttgcgcg tcggcctcga agaatgcctt gggcaggccg gcatcggcgt cggcgacctt
      181 gccggggcgg tcttcgggct gccgtgtcac ggggagaacc cggcagacac ggcccggctc
      241 gaccggctcg gcgccgacct cgtgggcggc cggcgaagcc tgtgcgtgaa cgacatggtg
      301 tgcggctggg ccggctcgct ggggctcgca gacggcgtca acgtcgtcgc cggcaccggc
      361 tcgatggcct acggccggcg cgccggcagc gaggcgcggt gcggtggctg gggggagatc
      421 gtcggcgacg aggggtcggc gttctggatc ggccggcgcc tcctcgctct cttctcgcac
      481 atgagcgacg ggcgcacccc gcggggcgcc ttgtacgaga tcgtgcgctc ccgggtggtg
      541 gtcggcgaca ccgacctcga ggtggtgccc cgattcgtcg cgggagcacc cgagctgcgc
      601 cgccgggtgg ccgccctcgc gccggccgcg gcagaggccg ccctcgcggg cgacgagggc
      661 gcggccgaca tcttgcgtgt ggcggggcga gagctggctc gacttgcgcg ggcgagcgcc
      721 atggcgctcg ggtactccgc aggcgaggcg atccgggtct cgtattcggg cggtgtgttc
      781 ggggccggcc cggttctggg gtcgttccga tcggccctga cgaacgccgg gtctttcacc
      841 gtgaccgcac ccgtcacctc cccggtgttc ggcgcgctcg cgtacgccgc gcgcgaattc
      901 ggggagggca tagacctcgc cgcgctcgcc cgcgcggacg gtcggcggcg aggggtgccg
      961 gggtgacgcc gtagcccggc ggccgggggc cgcctcagac gaagacgcaa tcgaagacgc
     1021 cggcccgggc ggccggtgac atgtcataag gagtatccga ccgtgaacac gttcgttgag
     1081 agtgagatcg cctcccagcc cgagatgtgg cgccgtgtcg ccgcgctggc acctgacgtc
     1141 gccgccatgc tgccgcaacc gggggagcgg gtcgcggtcg tcggctgcgg cacgagctgg
     1201 ttcatggcga tgagttacgc ctcggcccgc gaatccgccg gcctcggcct gaccgacgcg
     1261 ttcgccggct ccgagtaccc cgcctcccgc acctatgacc gcgtggtggc gatctctcgt
     1321 tccggcacga cgagcgagat catcgccctt ctcgagggac tgggcggccg ggagagcacg
     1381 gtcgtgactg gcgtcgccga cagcccggtg acgacgctcg ccacccacac ggtggcactg
     1441 cccttcgccg acgaacgttc cgtcgtgcag acccggttcg cgacatcgac gctggcgctt
     1501 ttgcgatccg gcctgggcga cgacgtagcc tcgctcgcag accaggccga cgaggcgctt
     1561 cgggtggatg tcgagccgtt gctcgacgcg gaacaggtgg ccttcatcgg tcggggcctc
     1621 gcggtgggcc tcagcttcga ggcagcgttg aagacccggg aggcggcgca gttctgggcc
     1681 gagtcgtacc cggcgatgga ctaccggcac gggccgatcg cgatcgcgca gcccggccgc
     1741 ctgacctggt cactgggccc cgcgcctgag ggcctcgagg cacaggtggg cgagaccggc
     1801 gcccggttcg tgcatcacga cctcgacccg atggccggcc tcgtcgtcgc ccagcgcttc
     1861 gcgatcggcg tcgcgctgaa gcgcggcctc gaccccgacc acccgcgcca cctgacccgc
     1921 tcggtgatcc tcacctcgtg actgggcggg gccttctcgg cgtcgacgtg ggcggcaccg
     1981 gcatcaaggc gcttctggtc gacgccgccg gcgcctgggc gggtcggtgg cgtcggccga
     2041 cgccgcacgg cgacacgggc ggcgagcgca ccgcgcagac ggtcgcagaa ctggtcagcc
     2101 aggcccggag tgtcgcgacg ggccgagggt tcgacgtggc cgcggtgggc ctcgtggtgc
     2161 ccggcgtcgt cgaggccgct cggggcgtgt gccgcctggc cgtcaacctc ggctggaaag
     2221 atctccccat tcgacagatg gtggcgaagc gggtgggcct gcccgtcgcg ttcggccacg
     2281 atgtgcgtgc cggcgcgctg gccgaggctc gcaccggcgc cgcggcgggc cttcgcgggc
     2341 tggcgatgtt cttgccggtc ggcaccggta tttcggccgc ctacacccgg ggcggggagc
     2401 cgttcgagct cggccccgcc gcgggcgagg tcggccagat cgtgctgcgc cgcggccccg
     2461 gcgccggcct ccgcgtcgaa gaggtcgcct ccgccgccgg catcgcccgg cgtgtcggcc
     2521 ggccgaacgc gctggccgtg gcggaggcgg ttcgggccgg cgacccggcg gccgtggccg
     2581 cgtggcggga ggccgtcgaa gtcctcgccg acgtggtcgc gtggagcgcc gccacgctcg
     2641 acccggaggt catcgtcgtc ggcggaggtc tcagcgcggc gggagacctc ttgttccgcc
     2701 cgctgcgcga cgcggtgtcg gagcgcgtcg acggcatcgt cgtccccagc ctgcgcgccg
     2761 cagtgcacgg cgaccgggcc gcgatggtcg gtgcgatact cctcgccggc gatctcgtcg
     2821 gcgcgggtcc tcgcggggtc ggccggcatg gcgccgccga gggggtcgga gcagacgtgc
     2881 cgaccggggg aaccgaagac gcgggggagg ccaggtgagc gggcatgtcg tcatcgtcac
     2941 ggccaatccg gccgtcgacg tcacgtatgg cctggcggtg aacaagccgg gcgagacgaa
     3001 cagggtgcag cgggtcgtgc gccgtgccgg tggcaagggc ctgaacgtcg cccacgtgct
     3061 caccgccctc ggcgtcgagg cccgcagcgt gctgcccgcg ggtggtgacg cgggctcgtg
     3121 gctgcgcgca gagttgtccc gcgacccggc agtcggcgcg atcgacgtgg tgccgatcgg
     3181 gggtctcacc cgacagacag tcgccattgt cgccgatgcg gggcatccca ccctgtacgt
     3241 cgagcccggg ccacgcctca cggccgagga atgggaccgg gtggccgacg ctgtcgccaa
     3301 ggcctgcgag ggcgccgcct tcctcgtcgt ctccggctcc ctgccggctg gcgctccggg
     3361 cgacctggtg ggccggtggg tgcgggccgc ccgtgcccgt ggcgcccggt cgctcgtcga
     3421 ctgctcgggg ccggcgctcg tggcttcggc agaggctgaa gccgacatca tcaaaccgaa
     3481 tgaggcggag ctcctcgccg cgaccggcgc cgcgactctc gaggcggggg tcgcgcagtt
     3541 gcgacgttgt ggggcggggc ggctcgtcgt gtcacgcggg gcggaggggt tgctcgtctt
     3601 cgatggcgag gctcgtgccc tggtacccgc cgtgccgggg gtgaccggca acccgacggg
     3661 tgccggcgac gccgccgctg cgggcctcgt cgcggcgctc gttcgtgggt atccgctcga
     3721 ggcggccgcc cggtgggccg gggccgcagg cgccgccgcc gtgcgtcagc cggtcgccgg
     3781 cgtcatcgac acggccgact tcgacgccta cgtcgaggcc ttgggtgggc cccgccggtg
     3841 agcccgaaca ccgggaccgg ctcccggccg gtgaatccga acgccacgcg gcgccgcagg
     3901 ccggcgaatg cggcgttcgg ggccggcccg ggcgtgtggg cgcaggccga gacgagaatt
     3961 cgtgtggctg cggctgaagc cgcgacagga aggaaggtct gatcgtgaca ccgaatccag
     4021 gagaggcgca gggcgatctg gccgtgccgg ctgccgccac ggccggcatg ggcttgccgg
     4081 gcgaggtcgg aacgcgggcc gaggccctgc cgccgcgccc cgtcacggag ctgttgcggg
     4141 ccttggctgc agaccggcac ggcgtcggcg cgttcaacgt cgtgctcctg gagcacgccg
     4201 aggcaatcgt cgccggggcg gcggaggccg ggttgccgac cttgctgcag atcagcgaga
     4261 actgcgtccg ctaccacggc gcgctgcgcc ccatcgtcgc ctcgtgccgc gagctggcgg
     4321 cctcctgccc ggtgccggtc ttcgtgcatc tcgaccacat caccgaccgc cgcctcgtcg
     4381 aggagggact agcggccggg gtcgacagcg tcatgttcga cggctcgaag ctgccctatg
     4441 acgagaacgt cgccgccacc gcggccgtcg tgcgggcgtg ccacgcccgc ggcgtcagcg
     4501 tcgaggccga gatcggcgag gtcggcggca aagacggcgt gcatgcgccc ggcgtgcgca
     4561 cgagacccgg cgaggcggcg cagttcgctc tggcgacggg ggtggatgcg ctcgccgtcg
     4621 ccgtcggcac gtcgcacgcg atgacctccc gcaccgcgag cgtcgaccac gatctcatct
     4681 cccgaatagc cgccgccgtc gccgtgcccc tcgtcttgca cggctcctcc ggggtgcccg
     4741 acggcgacct tgcgaaagcg gtcgcggccg gcatgacgaa ggtgaacatt tcgacccatc
     4801 tgaacaagct cttcaccggg gagatccggc gggttttggg ccccgacccc gacgtcgtcg
     4861 acccgcgcaa atatgtcgcc cccgcccgca ccgtcgtcgc cgcagaggtc gcgcggctgc
     4921 tgcggcttct cgcctgagaa tcggccgggc cccgggccgg gctgcggcat agcccccgtc
     4981 cgccacgccg ggttcctgca cagggcagag ctgcccgcgg gccagtcgcc gccgtccgcc
     5041 cgcgatatcg gccaccccac cggtatggca atgttgttca cgaggcgatg cttgtgtccg
     5101 agatcttgca catgcccgac catgcgggcg gcacatcgca ccgccctacc cccggggcac
     5161 cgccgcgtgt ccaccgggcg agcggttggc gagtcgccgc catggtcgcc agccacctgt
     5221 tcgacgacct ttatcaaggc gccgtgccgg cgatgctgcc gttcttcgtc gccgagcggc
     5281 attacgggta cgtggcggcg gccggcatca cgcttgcggc caccctgctc tcctccgtgg
     5341 tgcagccgct cttcgggctg ctcaccgacc ggcatcccat gccgtggctc gtgcctgcgg
     5401 ggctctctgt ggcgggcctc ggcatcgcgc tctccgggct cgtcgactcc tatctgtgga
     5461 cctggtgcgc catcgcgctc tctggggtcg gcgtcgcctc ctaccatccg gaatcgaccc
     5521 gcttggcccg cgccgcctcc gacggcagcc atgtcggcat gagctggtat tcgctgggcg
     5581 gcaacgtcgg cttcgctctc gggccgatcg tggtcgcccc cgtcgtctcg gcgttcggcc
     5641 ttccggcgac gccgctactc atcattcccg ccgtggtggc aggcgtggcc ctcgcgctgc
     5701 tcctgcggcc gcttgccagc aaggcacagg tcttcgctgc cggagcgttg gcgggcctcg
     5761 aagacgactg gagacagttc agcctgctcg cggtcatcgt cacaggccgc tccatcgtgg
     5821 ggttcgccct caacaccttt ctcgccctgt gggtcatcga gcatctgcat gcgcacggcc
     5881 agttcgaggg gcaggtcgcg ctcgtgctgt atttcggctg cggcgcggtc ggcaccctcg
     5941 cggggggctt tctggcgagg cggtgggagc gcgtgcgcat cttgcggctg tcgtacctgc
     6001 tctccgtgcc ggctctggcc ggcatcggcc tcgctcccgg cgcgttcgtc ttcttcttca
     6061 tcgcggcctc gggcatcgtc ttgttcctgc cgttctcgtt gcaggtgacg ctcgggcaag
     6121 attatctgcc cacccgcatc ggcacggcga gcggggtgac gctcgggctg tcgatcagca
     6181 tcgggggcct cgccgccccg gcgatcggcg cgttggcaga ggcgatcggg ctcgaagggg
     6241 cgattcttgt tctgatcgtc gtgcccgccg cctgctggct gctgtcgctg tggattcgtg
     6301 agccgcggca ggtcaccggg tgaggcggcg ttcgggccct ggccgcggcc ggcgccccca
     6361 ccgacacgct caccttccag ggcggcacgc cgatagagcg gtatcgacgc ggcggcggcg
     6421 cggggttcgc ggccggcgcg cggcgcgcca tggagggccg gatgaatgcg gtggatgctg
     6481 tgggtgagcc tcgggcgtct cgtgcgctct tcccgtcgat cggcgtcgtg acgatcgtcg
     6541 tcgcagacga gttcgagacg cggcgggtca tgacgatgct ggccgagaag tcgtgtctgc
     6601 aagaggtgcg ggtggtggtc ggcctcgagg cattgccgca ggaggggccg acgttggttg
     6661 tcggcccgct gacgcggcag ctggactacg acgacctgtc gcgccccggc ctcgactttc
     6721 tgccggagcc ggtcgacgcg gaagccctcg tcgaccgggt gcgggtgtgc gcggtgcgcg
     6781 gccttgccga gaaggagccg tacgggccgg cggattacgg ccggctggtg caagagatcg
     6841 ccgattcctt gaacgtggcg gtgctgttct tcgacacggc cgggcatccg cggctacgta
     6901 accgcatggt gcgtgaggtg ctggccctgg ccggctatga ccccgccacg ggcctggccg
     6961 agcaggtgta cgcggccgat cggcacacac ccgttaaacg cggccggaac atcctcaccg
     7021 aggcgctcgc ggggcacggc cgtggcgtcg tctactgggt agggcatccg agcgaggccg
     7081 agcggcagcg tgcgatcatc accgaggcgc acacgatcca gcggcccgac ggtcaggcgc
     7141 tgggcaccgc catgctcgtc tacgatgtga ccgagacggt gctcttgacc gaggagcagg
     7201 ccgactacgt ctccgcgctc tcgcacgagc tgcgcacccc gctgaccgcg gcgatgggct
     7261 acctcgacct gatcgccgag gaccacgaca tcgaacggtt gggcatcgcc gacgagttca
     7321 cgatcgccca gcgcagcatg agccagttgg ccgggcttct cgagcgattc tcgagcgtgg
     7381 gcctgcgccg cggcgcgctc gtgctcaccc cgaccgatct cgccgccctc gcaggccagg
     7441 ccgcccatgc ggtgcggccg atcgccgacc gggcgggtgt cgccttgcac accaggctgc
     7501 ccgacacgtc gctgttgggg cgggccgacc cgttacggct gcgccaggtg ttcgacaacg
     7561 tgctctcgaa cgccgtgaaa tacacgccgg aaggcggcag tgtcacgttc gccctagacc
     7621 gggagggcga cgaggcggtc atcacagtca ccgacaccgg catcggcatc gatccggccg
     7681 accagcaccg ggtcttcgac cgcttctacc gcacgcccag cctgcgtggc ggttcgatcg
     7741 gcgggcacgg catcgggctg gcgatcgcac gcacggtcac ccaggcgcac ggcggaacca
     7801 tccgcgtgag gagcacgccg ggtctcggca gcacattcac catccgcctg cccctgcgcc
     7861 cgaagggctc caccctgccg cagatccacg acgagaccga gtgaagagcg ggtgaggccc
     7921 gacgcgtcgc ggtcgacgaa gagagttgcg gcgggcctgc gatgcgggtg cggccgtgct
     7981 cgatgctcgg gcctgccgcg gcacaggggt cgcgacatct ctctacaatg ggtagagtgt
     8041 gaagacgcta tgagggtcgt gcctcgggcg tcttgtcggg gcaccgccgg cggagcaagc
     8101 ccggtgccgt gagtggtcgg tgtcgcgcgc gagcgtcgcc gtatgggtcg tgtggagcga
     8161 gccagaaacg ggaggtcctc cgtatgccta cgttcgggtc ggatggttcg cgccgcgggc
     8221 gcgtgcgaga gaagctctgg cggaccgtgt gcgcgggagt cggccgggcc ctgcgacgcg
     8281 ggcgccgcgt gtctcgcgaa cgtggtgtcg tgggcggcac gccgggccgc gtcacgaggc
     8341 tggcggccgc gatggtggca ggcctggcgc tggcgctcgc cggcccgctc tctgccagcg
     8401 cacacgtgcg ggtgagcccc gacacggcca ctcccggcgg ctatgccctg ctcacattcc
     8461 gtgtgccgac ggaatccgcc accgcgagca cggtgcgcct cgaggtcgac cttccgaagg
     8521 acacgccgtt cagttccgtc tcttacgagc cggtggcggg ctgggcgacg agcgtcacca
     8581 cgtcgacgct tgcgacgccc gtgaagacga agaacgccac catcaccgag gcagcgacaa
     8641 aagtcgtctg gacggcgacg agcaaggccg cacgcatcgc gccgggccag ttccggcagt
     8701 tcaccatcag tgccggcccg gtgcccgaga cgggcaaggt cgtgctgcct gcgcaccaga
     8761 cgtattccga tggcaccgtc gtcgactggg ccgagccgac gcctgcctcg ggcgtcgaac
     8821 cgcaacaccc ggcgccgacc gtctacatca ctgacgcgcc gcccggcgac gtgtcgtccg
     8881 cagccggggc cccgacggtg acggcgacgc cgcgggccga cggggcgagc gccctcgacg
     8941 tggccgccgg caggaatacg accgtggatg cggtggcgct cggctccggt ataggcggcc
     9001 tcgcgctggg cgcgatcgcc ctcgtcgtgg ccctcgccgg cctcgcgcac cggcgggcag
     9061 ggacgggccg gccgtgaatg tctcgtcggc tgttcgccga ccgggtcccg cgacgcgttc
     9121 gcgtcgcccg atccggaagg tcgtcgtcgc gatgctcgcc ttggtggcgg gggtgttcct
     9181 cgtgctcggc ggggccgacg cggccagcgc ccacgactac ccggtgcgct ggtcaccggc
     9241 cgcgggctcg acagtgacca cgccgccgac gacggtgtcg atcaccttcg acgacctcgt
     9301 gctcgatctt tctggcgact ctcatacctc ccttctgcag gtcacgcaca catcccaggg
     9361 ggcgacccgg tacttcgcga cgggttgcac ggtgacgaag gggcgcgagg ttagcgttcc
     9421 ggtcgtgctc ggcccggccg gcgactacaa ggtctcgtac cagatcgtct cggcagacgg
     9481 ccatgtggtc tcgggcaact cgaccttcca ttaccgcccg gcgtcgggca cgacccaggc
     9541 ggccggcacc tcgagcccgg cctgcgaggc cgccggcccc gcccgggccg ccagcccgtc
     9601 cgctacggca ggccgctccg ggggcggtgt gtccggcacc tccggctccg ccggcctgcc
     9661 cttcggcatc gtcctgggtg tcgcgggcac catcgtcgcg ctggccgtca tcgccgtggt
     9721 cgtcgtgctc ttcacgacgc gccgcgcgcg cccggcgagc cgtacgtcac gctggcggcc
     9781 gcaggacggg gaggctcgcg gcgccgacgg cacaccgcac ggcgaggcga ccggcggcgg
     9841 ccctggcggg ggcggccgcg aggcgagcgg tggcgaggcc gacggctctg gcgccgaccc
     9901 cgaggaatga tcgacccggc cggcgggtgg tcggtcaaga gttcggttcg gcgcccctag
     9961 ccgaggtacg cgtcgatcaa ctcacgccct tgggaatcca cctcgctcgc ggcgctctgc
    10021 ctgacgatgc ggccgccgct caacacgtag acgtggtcgc agacctgtag cgcgaggtcg
    10081 acgcgctgct cgacgagcag gatggattgg cctcgctcac gcagcgcgat cagcgcgttc
    10141 gccatgtctt ggatcgcgag cggcgcgagg cccaacgacg gctcgtcaag cagcatgagt
    10201 gacggcgcgc tcatcatcat gcggccgatc gcgaccatct gttgttcccc gccgctcatg
    10261 gcggtcgccg gccgcgaccg gtatcgctcc accacgggga agaggtcgaa gaccgattgc
    10321 agaagctcct ccgtgcgctc cttgtcgcgg tgcaggtgca agccgccgag gcgcaggttg
    10381 tcttcgacgc tctggccgag gaacagtcgc cttccttcgg ggacgagggc gacgtgcccg
    10441 cgggcgacct tgtgggtcgg catcgccgag acgtcgacgc cgtcaacgac gatcgtgccc
    10501 ctccggtgtt tcaacatgcc catgatggcg cgcagaaccg tcgtcttgcc cgctccgttc
    10561 ggcccgagca gggcgaccgc gtgacccttt gcgacgttca gcgcgacgtc gtggacgacg
    10621 tcgaatcggc cgtatcctgc aaagagcccc gtgacgttca gtgcttcaga cgtgtgctcg
    10681 ttcattggcc cagatacacc tctcgcactc gttcgttctg ccgaacctcg tcggccgggc
    10741 cgctcgccac cagcttgccc gccgcgagca ccgtcacgtc gtcgcagacg tcgaagatga
    10801 actgtgtctg gtgctcgacg atcaccatcg tcacgccacg tgagcgtaag ttgccgacga
    10861 tgtccgtcac ctgggcgacc tcgctcggag agaggccggc gagcggctcg tcgaggatga
    10921 ggatgtctgg cccgccgaca caggccgccg cgagctgcgt caactgttcg accccgtgcg
    10981 gcacgtcggc cacgcgcacg gaattccagc tggcggcgag cccggcgtcg cttatcgccg
    11041 cgcccgcctc ggcccgcatg cgccgatgat cgcgtaacgc cgtcggcagg agcggccaga
    11101 tcatagagcg cacggcgatc cgcggaatgc gatgatacag gccgatcgtg acgctctcac
    11161 gaatcgagag ctcgtcagcg agcaccgcgg tctggaaggt gcgcaacagc ccgaacctcg
    11221 gccgccggta ggcgggcagg ccgtcgacgc gccgcccacc tatcgtgatc gtgcccgact
    11281 ctggctgaag cgccccagag atgagattga cgagagtgct cttgcccgat ccgttcggcc
    11341 cgatcagccc gtggatggtg ccgcgccgca cgcgcagatc caccccgtcg acgacggtgt
    11401 tccctttaat ataggcaaac tggattccct ggcattcgag ggcatgcccg acgggtggcg
    11461 gcgtgttcgt cacgctcggg ccggcggtgt cgagacggcc ttgccccgcc cgagcgtcgc
    11521 ctaccgccgg cgtcttttcc ggtcggcgtc tcacgaagcg cctccagagc caacctgcca
    11581 accccatcac accgccgggg aaaagcacga tgacgagcag gaagatcagg ccgaagatag
    11641 cttcttgcac gagcggatcg atggcgacga ccacggggaa ggcactcacg aacgccgccc
    11701 cgatgagcgg gccgacccgg gtgttgatgc cgccgaagat caccatgacg aggatgttca
    11761 ccgacatcgt ccacgtgaac tggccgggcg agatgtagcc ggtgaacgag aacgcccatc
    11821 ccgccatcgc ggcgagcccc gccgagaggg cgaagacgcc caccttggtg gccgcggtgc
    11881 gcacgccgtt cgccgccgcg aacggttccg cgtcgcggat cgccagaaga acgggataca
    11941 tcgccgaggc cctgatgctc cacacgacga cgaccgcggc caggaggacg aggcctacgc
    12001 accagaccaa cgatgagccg aggaaggaga cggattgtgg aaagtcgggc agagacgggc
    12061 ccgcgattcc gttgagcccg cccgtgaacg agaagtcggt gagcacgacc gtgaccacga
    12121 gcgagaagat gaaggtcatg atcgcgaagt agaggccgct gacccgcagc gagagggtgc
    12181 cgaggactgc cccgatgatc ccgacaccga cgatcaccac cggcagcaag acgaacatgt
    12241 tccagccggc gtggtagagc agggtcgtgc cgtacgcgcc aagggcgaag aggcccgcat
    12301 gtcccagcga gtacagcccg gtcatgcccg cgacgagatc caggctcacg gcgagcacgg
    12361 cgtagacgcc gccggtcacg aagaggcttt gtgcgaagaa gttgccgtgc gcgaccccgc
    12421 tgatggagag gaggtatgcg agggttatcg cgagtgtcgt gacgcgcatc agcctgctgc
    12481 ggccggaggg ggcgggtgcg ccctgtcgtg tttgccgccg actcatcgtc atcgtcatga
    12541 ctgcaccccc acgctcgctc ggcgggctcg gaggctgcga gagagccgcg tgaagaggtt
    12601 gcccgacgag aacaggccgt gcggcgtgat cagcaacacg accacgacga cgacgaacgg
    12661 gaaccagtcg gaggcctggg aattgatgta cgtgttcgcg gccaccgaga ggatgccgag
    12721 cagcacgccg ccgcccatcg ccgccgccgg cttgtcgatc cctccgatca tcaaggcgat
    12781 gaagcctgcg ataccgtacg tgtcgcccag atacgggttc gcgaaggtta tcgggccgac
    12841 gagaacgccg gcgatccccg cgagaaggcc gccgatggcg aacgccacga ccgcgtagcc
    12901 ggtggcgttc gcgccgacgg ccgaggccat ctcggggtca ccggagatcg ccatgcccac
    12961 tttgccgaaa agagtgcggg tgcggaagag ctcgaaggcg acgagcacca ccgcggccac
    13021 gatgacggcg agcatctctt gccacgtcgt cgatgcgcca ccgaagcgaa tcttctcgtg
    13081 gtagaagagc ggcgggaacg ctctcgaggt cgggccgaag atcatcgccg cgaccgacgc
    13141 cagcacgatg gcgacgccga gggtgctgac gagccagccg aagcccgtgc gatccacctt
    13201 gagcaccggt gcgacggcgc cgtagtagat ggccacgccg aacccggtcg agagtatgag
    13261 gccgacgacg atcgcaatca acgtcggcaa cccgatcgcg acgagaccgg aaacggccat
    13321 gatggagacc atcaacagct ggccggtggc gaaattgatc accttggtca cgtagaagac
    13381 cacggcgaag gccatggcga ctaagccgta tatcgccccg gtggcaaggc cactgagcaa
    13441 gacctgaagc atccctctcc cttgagcttt cgtggtgcgc cgcaccgacg gcgcggtggg
    13501 cgggccctcg gccgcatggt gaagacggcc gagggctcac cgcgtgtcaa ctcacaggaa
    13561 cccaggcgtc tgacgcggcg tcgtacttga cgacggtcag ctgttcgggg gtgagggttg
    13621 tgtgttgttt ctcggtgaag gtgaggctgc cggtcaggcc gtcgaagtcg gtgatcttgt
    13681 tcagctgcgt gcgcagcgac tccggctggg tcgacttgcc ctcttcgatc gccttcttga
    13741 ggatgtatac ggcgtcgtag gtcagttcgg cgaacgcggt cgcgtcgacg ttgaacttct
    13801 tcttgtagtc cgcgcggaac ttggtcaggc gcgcgttggg gttcatgtcc gtcgtgatca
    13861 aggcgcagat catgccgtca ccctgctttt tcgcgagccc tgagaaggtc gtgtcggagc
    13921 aggtctcgtc gttgccgagg aaggtgaagt tagcgccttc cgtctcggcc tcttgcatga
    13981 acgatgcctg gtcttgcggt gtcagccaca cgtcgacgca gtcggcgccg gatttcttga
    14041 tcttgctgat ctccgacttc agcccgacgg tggcaccggt cgaccagttc tccgtgatcg
    14101 tctcggtgag cgcgagcttc ttgttggctg ccttgtaggc cagctggatt ccggcgagac
    14161 cgcccatgcc gtatccgctc gggtcgtgca gcacggcgag cccggtgcag ttcttcagtg
    14221 cgtattcgcc gagcttctga ccccaagcaa acgtgttgag gctggtgctc catgcccagg
    14281 cgtaggggtc ggagtcgggg cccttcgggt ataccgacag gccaccgtca tcaacggggc
    14341 cgagcaccgg gaacttcttc tggtcggcca gcgccgcaac cgtcgggcct tggtcaggcg
    14401 agccgagaat cgcgaccgcc ccctggcttt gcagcttgct gaacagctgg gaggccttgg
    14461 tggcgtcggc gccgtcgtca taaccttgga acttcagctt cttgccgaga accgttccgg
    14521 cgctgttgat ctcgtcgatg gccagttcgg cgccgttgaa cgccgcttgc ccggtcgtgc
    14581 cataggcgcc ggtggtgccg gcgatgccac cgaccagaat ggtgccacca cctccgccac
    14641 tgctgctgcc ggtgctgccg cctccgccag aacatccggc gagagccagg gcggcgagcg
    14701 ccgcaatgct cgccagtgcg atcttcttca tgtcgttcac ttccttgtgt tcgggtgttt
    14761 cgctgtgtcg gacggtgtcg tcacgcctga tctccggtgt cggtgtcgtc ggtgagacct
    14821 cgtgcgggat ttccttggtg tgtgcgctgc tgtgctgggc cgcgcccgtg gtgcacgtgc
    14881 tgtgccggcc gtgcccgtgt cgttcagcct actgtacgat tgtatgacga taagtcatgt
    14941 tgggctatac cggggtctgt ccggcggccc tcccgttcga ggcctcgggc gtcgagcact
    15001 ttccgtgatg tggcggcgag gcgccggggt tgatgggcgc cggcccgggg gggtctgtgc
    15061 tggccttggt gcaggccgat cgtcggtcga gcggcaggcg gcggccgctg ccgggctcgt
    15121 gcaccgagcc caaaaggtgt cttaagttgg catattatcg tatgacagta tgatcacgca
    15181 tgctatcgtg tgaccgtcga gacgtgtgag tatcgcggtc tcggcgcctg tgccacgggt
    15241 gacacgaccc gggcgtgaag agcctcaagg aggagacgca actatgggac ggttagacgg
    15301 gaaagtggcc ctcattt
//
LOCUS       DASTNL010000201         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_889237, whole genome shotgun sequence.
ACCESSION   DASTNL010000201 DASTNL010000000
VERSION     DASTNL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889237"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..866
                     /locus_tag="VFQ96_06285"
     CDS             105..866
                     /locus_tag="VFQ96_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0257431.1"
                     /translation="MSQPEQTLPLAGRRALVTGASRGVGADTARLLAGLGAKVAVNYR
                     AKKPRADKVVADIAAAGGEAVAISADLTDAEAVRAMLGTVTDAWGGIDVLVLNASGGM
                     EGDRGPDYALRLNRDAQLGILRAAVPTMPAGSRVVFVTSHQAHFIRVAKTMPEYDAVA
                     RSKRAGEDALRAELPDLAARGIGFVVVSGDMIEGTITAKLLERINPGAIESRRKAAGR
                     LYNVAEFAAEVVAAVTEPVPPDNIRLVGDTSGFAS"
     gene            1421..1642
                     /locus_tag="VFQ96_06290"
     CDS             1421..1642
                     /locus_tag="VFQ96_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257432.1"
                     /translation="MRTLYLRGVPEEVVGRLRRLAEEAGTSVSAFAVRELTELSRRAD
                     NPDLLGILPDLGVQTEEIVAGVERGRDGR"
     gene            1639..2022
                     /locus_tag="VFQ96_06295"
     CDS             1639..2022
                     /locus_tag="VFQ96_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0257433.1"
                     /translation="MIVLDASAALSALLNDGPARGLVATEDLHSPYLVDSEVASGLRR
                     HVRSGGVAMGDGWAALDVWRRLGLTRYALNGLLGRIWDLRDNLSAYDAGYVALAEALR
                     CRLVTADSRISRAPGIRCAVTVVPR"
     gene            2140..2577
                     /locus_tag="VFQ96_06300"
     CDS             2140..2577
                     /locus_tag="VFQ96_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0257434.1"
                     /translation="MDQRLHFLTLATGDLGAARGFYCDGLGWTPLYDAPGEILFFQVA
                     PGLMLGLFDARKFNEDLPRSGSTTGVIGVTLSHNVASRDEVGTTVASLIAAGGTLVKP
                     PQEGAFGGIFHAHVADPNGVVWEIAHNPAWHVDDAGNVSFDAA"
     gene            2872..3288
                     /locus_tag="VFQ96_06305"
     CDS             2872..3288
                     /locus_tag="VFQ96_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257435.1"
                     /translation="MTSDLDSGAESSTELRRRRVFRVRLWIGTGCYLLTSAALYQWGH
                     EAGPWRVVWAILPLFFMAWVVAVIVLRVRQMDEYQVKLFFPGLAVGFTVAVFAALTVG
                     TLGAAGFDVPNGGWPVALAGILAWEFTNLLVGAPQA"
     gene            3302..3490
                     /locus_tag="VFQ96_06310"
     CDS             3302..3490
                     /locus_tag="VFQ96_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0257436.1"
                     /translation="MKNLIRAEREERGWTQAVLAEHLGVSRQTVVALETGRYDPSLPL
                     AFRISGLFDKPIEALFLP"
     gene            complement(3521..4570)
                     /gene="trpD"
                     /locus_tag="VFQ96_06315"
     CDS             complement(3521..4570)
                     /gene="trpD"
                     /locus_tag="VFQ96_06315"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU0257437.1"
                     /translation="MPDSVTWPSLLGTLLEKKDLSVSEAAWAMDQTMSGQVTDAELAG
                     FLVALRAKGETVNEVVGFRDAILSHALPLAVDPVALDIVGTGGDRFGTVNISTMAAIV
                     AAGAGVPVLKHGNRAASSRSGSSDVLSALGVDLTLSPERVAEVFRQTGISFVYASKFH
                     PGFGHAGGVRHELGVPTVFNFLGPLCNPARPEASAVGVSRLDRIPLMVGVFQTRGASA
                     LVFRGDDGLDELTTTGHSHVWEVTRGSVTEHDLDPRDLDIPLAHIDDLRGGEAAENAA
                     AVRAVLAGEKGPVRDIVILNAAAGLVAFDLVGEPEETRLSIVDRFRRKMAIAAQSIDS
                     GAAQAKLERWVAATH"
BASE COUNT          705 a         1588 c         1637 g          810 t
ORIGIN      
        1 gtccggccag gcgcccgaga cgcgccgggc ggggcggggg agtgcctgcc cgcttggtag
       61 tctcgttgcg cgcccacccg gggtgccgaa agacgaggaa cgtcatgtct cagcccgaac
      121 agaccctgcc gctggcaggc agacgcgccc tcgtcacggg ggcgtcgcgg ggtgtcggtg
      181 ccgacaccgc gcgcctcctg gccggcctgg gggcgaaggt ggcggtgaac tatcgtgcca
      241 aaaaaccgcg cgccgacaag gtcgtcgccg acatcgcagc ggccggggga gaggcggtcg
      301 cgatctcggc cgacctgacc gacgccgagg ccgttcgggc gatgctcggc acggtgaccg
      361 acgcgtgggg cggcatcgac gtcctcgtct tgaatgcctc cggaggcatg gaaggcgacc
      421 gcggccccga ctacgcgctg cgcctgaacc gcgacgcgca gctgggcata ctccgcgccg
      481 ccgtgccgac catgccggca ggctcgcgcg tggtcttcgt gaccagccat caggcgcatt
      541 tcattcgggt cgctaagacc atgccggagt atgacgcggt ggcccgcagc aaacgtgccg
      601 gcgaagacgc cctgcgcgcc gagctgccgg acctcgcggc gcggggtatc ggcttcgtcg
      661 ttgtctccgg cgacatgata gagggaacca tcaccgcgaa gctcctggag cgcatcaacc
      721 cgggtgccat cgagtcgcgc cgtaaggcgg cgggccggct gtacaacgtc gccgagttcg
      781 cggctgaggt ggttgcggct gtgaccgagc cggtgccgcc cgacaacatt cgcctcgtcg
      841 gcgacacgag cggcttcgcc tcctgaccgc cctccgtcgc ctcgtcgggg gagttgcgtg
      901 cgcgggggct cgagaacgag tccggccggc acttcaggca gctctccggc ggccagcagc
      961 agcgcctcgc gctgctcacc gccacggcat cctcctcacc gcacattcga tggaggaggc
     1021 ctaggccgta tgcgaccgcg tggcgatcat cgaccacggc cggctcctca ccatcggcgc
     1081 gcccgacgag ttggtcgaac ggcatcgaga cgacccccgg gtgctcgccg ccgcgcacgg
     1141 cgccccaacc ttggaagatg tcttcatcgg tttgacggga agtgacatcc gtgagtgcgt
     1201 gagtgatagg ggcgtgaggg aagatggcgt gaccgcatgg ggacgcgtca cccgcccagg
     1261 gctcgggctc gcgttcgtct cgccgcctgc gtcggctaca tcgtcgtgtt cgccttcctc
     1321 ggcatccgct ggtcccatgg ggccaaccgc tgacggcctg cgacgaagtg ggggattcta
     1381 actctagcat taagcgatat aagtgctatc attgtgcgac atgaggacct tgtatctgcg
     1441 tggcgttccc gaggaggtcg tgggacgcct tcggcggctt gcggaggagg cgggaacctc
     1501 cgtcagtgcc tttgccgtac gggagttgac ggagctgtcc cgccgggccg acaaccctga
     1561 tctcctcgga attcttcccg acctcggggt gcagacggag gagatcgtcg cgggggtcga
     1621 gcggggcaga gacggcagat gatcgttctc gacgcctcgg cggccctgtc ggcgctcctg
     1681 aatgacggcc ctgcccgggg tctcgtcgcg acggaagacc tgcattcgcc ctaccttgtc
     1741 gactccgagg tcgccagcgg gctgcggcgg cacgtgcggt ccggcggcgt cgcgatgggc
     1801 gacggctggg cggccctcga cgtctggcgc aggctcggcc tcaccaggta cgccctgaac
     1861 gggcttcttg gccgcatttg ggatctgcgc gacaatctct ctgcctacga cgcgggctac
     1921 gtcgcgctcg ccgaggcgtt acgttgccgc ctcgtgacgg ccgactcccg catcagccgt
     1981 gcccccggca tacgttgcgc agtgaccgtc gtgccgcgct gagtcgccgg acggcgcgca
     2041 gcgcgcgatc gtccgaacga aatgtgaccg ataatgtaca ttatgtcagt tttgaatctc
     2101 ctctcgaccg gctggcgggg ttcttgcgag actggagcga tggaccaacg cctgcacttc
     2161 ctcaccctcg cgaccggcga cctcggtgcg gcgcgcggct tttattgcga cgggctcggc
     2221 tggacgccgc tctacgacgc gcccggtgag atcttgttct ttcaggtcgc gccgggcctc
     2281 atgcttggcc tgttcgacgc ccgcaagttc aacgaagatc tcccgcgtag cggctccacg
     2341 acgggcgtca tcggcgtgac gctctcgcac aacgtcgcca gccgagacga ggtgggtacg
     2401 accgtggcct ctcttatcgc cgcgggcggc accctcgtga agcctcccca agaaggcgcc
     2461 ttcggtggca tcttccacgc ccacgtcgcc gatccgaacg gcgtggtctg ggagatcgcg
     2521 cacaatccgg cctggcacgt cgacgacgcg gggaacgtga gcttcgacgc ggcgtgaggc
     2581 cgcaccgtga tcggccggtt gcatccggcg cggcctcaag cggcggcgac agggaaggcc
     2641 tccctcccgg tcttctaacc cacgcagcca gctctcgcgg ctcacgaacg gctctgcgag
     2701 ttctgaataa tgaatcacga tgcccggctt cgccatggcg ggcagccggt cggtgtcgtg
     2761 cttattaagt gcggctaata cctcgatcgg ggtccccgcg ttcatctcgt taccggcgca
     2821 acgatgacaa gcttccttga ctccacgtgc ctactcactc atagtgagag aatgacaagc
     2881 gaccttgact ccggggctga gtcttcgacc gagctgcgcc gtcggcgcgt ctttcgtgta
     2941 cgcctctgga tcggtacagg ctgttaccta ctgacgagcg ccgcgctcta ccagtggggc
     3001 cacgaggccg gcccgtggcg ggtcgtctgg gccatactgc cgttgttctt catggcgtgg
     3061 gttgtcgcgg tcatcgtgct tcgcgtgcgc cagatggatg aataccaggt caagctgttc
     3121 ttcccgggcc tggcggtcgg cttcaccgtc gcggtcttcg ccgccctcac cgtgggcaca
     3181 ctcggcgcgg cagggttcga cgttccgaac ggcggatggc ccgtggcgct cgccgggatc
     3241 ctcgcctggg agttcaccaa cctcctcgtc ggcgcgcccc aggcctgagg cgtgccgccc
     3301 gatgaagaac ctgatccgtg ccgagaggga agagcgcggc tggacccaag cggtgctggc
     3361 cgagcatctg ggtgtttcgc gccagaccgt ggtcgccctc gagacgggcc gttacgaccc
     3421 ctcgctcccc ctcgcgttcc gcatctctgg cctcttcgac aaacccatcg aggcactctt
     3481 cctcccctga ggctaagcgg gtaagaggcg ggggcggagg tcagtgggtc gccgcgaccc
     3541 agcgttcgag tttcgcctgg gccgcacccg agtcgatgga ttgggcggcg atcgccatct
     3601 ttcggcggaa gcggtcgacg atggagagcc tggtctcttc cggctcgccc accaggtcga
     3661 aggcgacgag gccggcggcc gcgttcagga tgacgatgtc gcgcaccggc cccttctccc
     3721 ccgccaagac agcgcggacg gcggcggcgt tctcggcggc ctcgccgccc cgcaggtcgt
     3781 cgatgtgggc gagggggatg tccaggtcgc gtgggtcgag gtcgtgttcg gtgaccgagc
     3841 cgcgggtgac ctcccagacg tggctgtgcc cggtcgttgt cagctcgtcg agcccgtcgt
     3901 cgccgcggaa gacgagcgcc gaggcgcccc gggtctggaa gacgccgacc atgagcggaa
     3961 tgcgatccag ccgtgagacg cctaccgcgg aggcctcggg gcgggccggg ttgcagagcg
     4021 ggccgagaaa gttgaacacg gtgggcacgc cgagctcgtg ccgtactccc cccgcgtggc
     4081 cgaagcccgg gtggaacttc gaggcgtaga cgaaggagat gcccgtctgg cggaagacct
     4141 cggcgacgcg ctccgggctg agagtgaggt cgacgccgag tgccgacaaa acgtcggagg
     4201 agccggaacg ggagctggcg gcacggttac cgtgcttcaa gaccggtacg cccgcgccgg
     4261 cggcgacgat cgcggccatc gtcgagatgt tgacggtgcc gaaccggtcg ccgcctgtgc
     4321 cgacgatgtc caacgccacg gggtcgactg caagcggcag cgcgtggctc aagatcgcgt
     4381 cgcggaagcc gacgacctcg ttcaccgtct cgcccttggc tcgcagggcc acgaggaagc
     4441 cggcgagttc cgcgtcggtg acctgcccgc tcatcgtctg gtccatggcc catgccgcct
     4501 cggagacgct caggtccttt ttctctagga gtgttccaag gagcgatggc caggtgacgg
     4561 aatcgggcat gacgctcatc ttatcggcgc tccggcgagc cgcctgcggc cctcgcaaga
     4621 gccctctgtg cccgtatttt ttccgccccg gctggcgggg cggtggtgac cgccgggagg
     4681 cctatccccc ataatggaat ctgtgacgag cactacggta tcttccacgg ccaatgcgcc
//
LOCUS       DASTNL010000202        15366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1444818, whole genome shotgun sequence.
ACCESSION   DASTNL010000202 DASTNL010000000
VERSION     DASTNL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 15366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15366
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444818"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..3212)
                     /locus_tag="VFQ96_06320"
     CDS             complement(207..3212)
                     /locus_tag="VFQ96_06320"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEU0257438.1"
                     /translation="MRRSLGLQALAAVGFAGLSTAGSRLEALADRLEVAADDLLPFFR
                     VAADPDSALVWLLRLEERAPRLIRRIVGDEASAARLFRLVGASDGLAQFLFRHPDECA
                     LVEVMPQLSSPAVPGEVERVRRRLLDAVGADAHGVASLAGETGWSALRVAYRRELVTV
                     ALYDLGQPGPVRGVDSVGRALAELAGAALEASLALARADVAGEGPGRFPADEVAATRL
                     TVIGMGKAGAGELNYVSDVDVVFACASARPHAVPTARVVTIATRLARQTIRGLSEVSD
                     EPALWPVDTNLRPEGKDGALVRTVESHAAYYRRWAKDWEFQALVKARPLAGDRALGRR
                     YVDAVLPLVWRGAERDGFVSRVQEMRRRVIRNIPPDERDRQIKLGEGGLRDIEFTVQL
                     LQLVHGRSDPHLRQADTLSALAALTARGYVGRAEARALAADYRTLRLLEHRLQLRHLA
                     RTHLMPSDEDGLRILARSSGLAQGPEGLAAIWSEVRSRVRDLHERLFYRPLLAAAAAT
                     PPEDFNLDSEQVAVRLQAIGFRNTRAALAHIAALSRGVTRRATIQRNLLPVLLQWLGE
                     GADPDGGLLAFRRLSESLGRTHWFLGMLRDSSGAAQNLTRVLSSAPFVADMFQRIPSA
                     VGWLDDEASLVPRPRQALDDQVGAILRRYDDPEMVAAALRTLRRQERLRLALNAIIRT
                     PDVARLGVGLADVDEAVISGALATAMRVAGGPGLEFGVVAMGRLGGREVGFGSDADVL
                     YVYRGAGEGDDSAPTASRVATTLVGLCADPVIPFDLDAGLRPEGRSGPLARSVEAYRA
                     YYTRWSATWEAQALLRARPIAGPADLLTEFVAMADQVRYPGGFTEESARDVKRVKARV
                     EAERLPHGADPRRHIKLGRGSLSDVEWLVQLRQLEHAADVPALRTTSTLAALSACVAA
                     GLIEESDAGVLRDSWLLCSRLRSALVLWGADADTLPADRERLEPVARLMGYGSHAASV
                     LEEAYLQATRRARGVFERLFYGPEPPSAE"
     gene            complement(3214..4554)
                     /locus_tag="VFQ96_06325"
     CDS             complement(3214..4554)
                     /locus_tag="VFQ96_06325"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU0257439.1"
                     /translation="MDKQRDFVLRTLEERGVKFVRLWFTDVIGSLKSVAIAPAEVESA
                     FAEGVGIDGSAVEGLTRLYEADVLLRPDPGTFQILPWRGETDPTARMFCDIYTPDGEP
                     AVADPRNVLKRTLEKAAGLGFTCYTHPEIEFYLLTSSKLDAEGHPTPVDAAGYFDNVP
                     GGTAHDFRRRSVRMLEDLGISVEFSHHESGPGQNEIDLRYADALATADNIMTFRAVVK
                     EVAIEQGAYATFMPKPLSGQPGSGMHTHLSLFEGDGNAFFEPGAQYQLSAVGRWFIAG
                     LLRYAPEITAVTNQFVNSYKRLWGGDEAPSFVCWGHNNRSALVRVPLYKPNKPQSSRV
                     EYRALDSAANPYLAYSLMIAAGLKGVEGHFDLPREAENNVWALSDEERRALGYTQLPV
                     NLNHAISIMEKSELVAETLGEQVFNFVLRNKRQDWQDYRAQVTPYELRSNLKML"
     gene            4721..5539
                     /gene="panB"
                     /locus_tag="VFQ96_06330"
     CDS             4721..5539
                     /gene="panB"
                     /locus_tag="VFQ96_06330"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0257440.1"
                     /translation="MAELTPKRVRTRYFRSAKEQGTRLVGLTSYDQLSAAIFDAAGID
                     FLLVGDSAANNVLGYETTLPATVDELIPLARAVAGAAHRALVIADLPFGSYEGGPGEA
                     LRSAVRFMKEAGVAAVKLEGGRRRAGEIREIVDAGIPVLAHIGFTPQSEHALGGHVIQ
                     GRGAGVERLLDDAHAVEDAGAFAVVLEMVPSEAARRVTAELTISTIGVGAGPDVDAQL
                     LVWTDWAGLTVGRVPRFVKKYADLATTLTDAVAAYRADVTEGRYPDEEHSYRES"
     gene            complement(5588..5770)
                     /locus_tag="VFQ96_06335"
     CDS             complement(5588..5770)
                     /locus_tag="VFQ96_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0257441.1"
                     /translation="MSDERRQFWYNSRTGEVEEGVLSPSAYRVGPFATRDAATHAPDL
                     LRENARRWREEDEAED"
     gene            complement(5812..6156)
                     /locus_tag="VFQ96_06340"
     CDS             complement(5812..6156)
                     /locus_tag="VFQ96_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257442.1"
                     /translation="MRHLKRNIAAFGAAAAIVASVGFVGVQSAVAEPAVSSASAAVSL
                     SQAPSATSSKVVTPAVAVQAAAPDSTSGNLNIGSPTPGGYVTSKAWVAVGAGIFLLLV
                     WSVSIFGYRRFH"
     gene            complement(6246..6428)
                     /locus_tag="VFQ96_06345"
     CDS             complement(6246..6428)
                     /locus_tag="VFQ96_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257443.1"
                     /translation="MSASKTRHPRMRSAAVRFLIAALVFLVLAIVTHLTFFAVLCAVS
                     AMSALWLWLSSVKDIR"
     gene            6566..6886
                     /locus_tag="VFQ96_06350"
     CDS             6566..6886
                     /locus_tag="VFQ96_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257444.1"
                     /translation="MTTVRGTVRSTADWEGEGSGPTAAAARAEAVRGLPDGFALVDVR
                     TLSAKPGEPVTMKAFGRGTASHEIEARGDDEPEALAALREAVPPGHLLLEVVIVPDGT
                     RPSA"
     gene            complement(7177..7698)
                     /locus_tag="VFQ96_06355"
     CDS             complement(7177..7698)
                     /locus_tag="VFQ96_06355"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU0257445.1"
                     /translation="MAQAAQAGRVPDGQVGLLWAQTLAGVIGAGGRIPWRLPEDQRRF
                     RRLTWGGRVVMGRRTWQSLPAAVRPLPGRENVVLTRRSTLDAPGAKVMHSVAEAVGPG
                     AWVIGGAEIYAAAMAWATLIEVTEVDIEVAGDTWAPKIDEARFMPERGAWQVSSAGLR
                     YRFVTWRRVAESR"
     gene            complement(7703..8521)
                     /locus_tag="VFQ96_06360"
     CDS             complement(7703..8521)
                     /locus_tag="VFQ96_06360"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU0257446.1"
                     /translation="MTSPEPAVVTAYEDLLRDVLVHGMPKGDRTGTGTRSVFGRQMRF
                     DLAAGFPLVTTKRVHFKSIAYELLWFLRGDSNVHWLQEHGVSIWNEWADENGDLGPIY
                     GVQWRRWPTPDGRHVDQLARVIDTLRRDPDSRRMIVSAWNVADLDAMALAPCHVLFQF
                     YARDGRLSCQVYQRSADMFLGVPFNIASYALLTVLVADQAGLVPAELVWTGGDCHIYD
                     NHLDAVRLQLTREALPAPTLSLARHAESIADYRYDDFLLEGYRHHEAIHAPVAV"
     gene            8785..9627
                     /locus_tag="VFQ96_06365"
     CDS             8785..9627
                     /locus_tag="VFQ96_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU0257447.1"
                     /translation="MSVSLAEFCTIADRLWPPDGAEEWDAPGLVCGDPAAEVSSVLLA
                     VDAVPATVEEATAGSHDLLLTHHPLLLRGVKSIAEDRYKGRVLAAAIRGGLAILAAHT
                     NADIVRSGTSAVLAAVLGLEDTRPILPAHGPEPRASGLGRVGRLPSAEGLRHLATRLA
                     RALPATAGGVRVAGEPEAQIRVVSLCAGAGDSLLSEPAVLASDVYITSDLRHHPASEA
                     REQARLAGGPALIDVSHWAAESLWLAGAAEELRRAVPGLRVAVSTLRTDPWDFTVESG
                     GQRR"
     gene            9624..10358
                     /locus_tag="VFQ96_06370"
     CDS             9624..10358
                     /locus_tag="VFQ96_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257448.1"
                     /translation="MKAAPEEQRRLLDVQRIDNAITKARSTADEAALGAELAGIEAEL
                     TRARHEQLERAGALEDGKTALARIESDVAAVDARAARDTARLHETSSLKDIHGLEAEL
                     RSLAERKDRLEDTQLEAMEELDGLGRAASAAESLVDETARRKADAEARRRTSAAERQS
                     RLEELSAERRDALVGLPEDLVALYEKQRARYGIGAALLRGGVSLGSNTTLNPADMQDI
                     RRAAPDDVILCPESSCILVRTEESGL"
     gene            10383..10741
                     /gene="rnpB"
                     /locus_tag="VFQ96_06375"
     ncRNA           10383..10741
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFQ96_06375"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(10744..11493)
                     /locus_tag="VFQ96_06380"
                     /pseudo
     CDS             complement(10744..11493)
                     /locus_tag="VFQ96_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(11551..11838)
                     /locus_tag="VFQ96_06385"
     CDS             complement(11551..11838)
                     /locus_tag="VFQ96_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257449.1"
                     /translation="MVRAESDGGVARRRRAGEIRGDRTRAVAGENVMGLPEPSTRTGA
                     DRLATLQQLYLDSIRERAQLRDFIARQRDEIARLRAGGPAGDAHPGVSESR"
     gene            complement(11907..13373)
                     /locus_tag="VFQ96_06390"
     CDS             complement(11907..13373)
                     /locus_tag="VFQ96_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257450.1"
                     /translation="MIVGVALLGLLARVFYSLRHGGPVYVGGYDSGVYYAAADALVHG
                     RLPYRDFLFIETPGVAVVTAPFAFLGAQTHDALGYGAANLFFAGIGAASSGMVAAVLR
                     RFGLPAAVFGGVFYAVTDIAVSSEQFIKLEPVATLLILIALLLLGDVRRPVGRRRMLV
                     AGLLLGLACGFKIWYVVPALVVVAATPGVRRRLRLLAAEAVGAVVIMLPFFLASPAVM
                     FREVVLDQLGRGRGGPSVVGRLHDILGGATVPHTATRLLGLTVGEITGGLAVLAVIAV
                     IFALRRPSARLYVWLLLSGLLVLIVAPSFFSYYTSLTSAPLALVLGAGFAELVGLLST
                     RSGRLVLTTTALAGLVLLNLPGQWHAHPGEHPSAGLRRAVATVPGCVISDDPEILAAT
                     DVLSRDLSDGCVLWPDVTGWTYDLDTGMHDGVYTPRTKNLAWQKHVTSYLTSGSAVMV
                     YRPETGLSEHTRRVVTAGPVLFRDGEWVLYATPHHLGG"
     gene            complement(13637..14644)
                     /locus_tag="VFQ96_06395"
     CDS             complement(13637..14644)
                     /locus_tag="VFQ96_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0257451.1"
                     /translation="MTATQPHAGDEAVVTRAARTIALRITAAASTIVVLIVAVSVAFI
                     LDQSRPSELLEKPAPGETKIYIASGDMFTALVVTGVVAVILAGLLSWVIARRVVAPLG
                     EALRIQRSFVADASHELRTPVAIISTRVEVLAQELEAGGDPHDDLAGLRRDVRALGEV
                     ITDLLLAASPVDGDTRADCPARPTVRAVLDDLAFLAGQNDVTLRLDGGEGLVAALPEA
                     TFRRCVVVLVDNAVKHSPSAGTIEVVLAPARRRFFELRVTDHGPGITGVDPARVFDRF
                     SHGQQAPSGTAARRGFGIGLALVRDIAGRYGGNVAVAETSPAGTTFVLTLPLASPRDA
                     R"
     gene            complement(14641..15321)
                     /locus_tag="VFQ96_06400"
     CDS             complement(14641..15321)
                     /locus_tag="VFQ96_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0257452.1"
                     /translation="MAEAEAGEASPSLLLVEDDPELGPLLLRALQSSFVVDLVADGEA
                     GLERALSGGYEVLVIDRRLPLLDGVSIVRKLRQSHITAPILLLTALGTTADKVEGLDA
                     GANDYLVKPFEYAELLARLRALTRRFDPRGRGLAVGEWMFYPEDACVVSPYIGRILLT
                     PRESTLLRVFAENPDATFTRETILRRVFSPGDKPGTVDTYVHYLRGKIDRDVILTVRR
                     KGYRLGSL"
BASE COUNT         2566 a         5432 c         5340 g         2028 t
ORIGIN      
        1 gctcaaggcg cgtgagcact gcctcacgat aactcatcgc cctcacccac cagctaggag
       61 ggacaggagg gcgcgtttga cggtggtccc gatccgatga tggttcctct tagcggtcca
      121 ctcgacattc ggctgtaggc acaactggac gcgcggtgca gggcgtcccg gctaagcggg
      181 tgaggcgcag cccaggccca ggccggtcac tctgccgagg gcggttcggg gccatagaac
      241 agtctttcga acacgccccg cgcgcggcgg gtcgcctgga ggtacgcctc ctcgagcacg
      301 gaagcagcgt gcgagccata ccccatcagc cgggcgaccg gctccaggcg ttcccggtcg
      361 gccggcagcg tgtcggcgtc agcaccccag aggacgaggg cggagcgcag cctggagcac
      421 aacagccacg agtcacgcag cacgcccgca tcagactcct cgatgaggcc ggccgcgaca
      481 caggctgaca gcgcggcgag cgtggaggtg gtgcgaagcg cgggcacgtc ggcggcatgc
      541 tccagctgcc gcagttgcac gagccactcg acgtcactca gcgagccccg ccccagcttg
      601 atatgacggc gcgggtcggc gccgtgcggc aggcgctcgg cctcgacacg cgccttcacc
      661 cgtttgacat cgcgggccga ctcctccgtg aagccacctg ggtagcgcac ctggtcagcc
      721 atcgcaacga actccgtcag gaggtcggcc ggcccggcga tcggcctcgc ccgcagcagc
      781 gcctgcgcct cccaggtcgc cgaccagcgg gtgtaatacg cgcggtaagc ctctacgctg
      841 cgcgccagcg ggcccgagcg accctccggc cgcaacccgg cgtcgaggtc gaacgggatg
      901 accgggtccg cgcacaaccc gacgagtgtc gtcgccaccc ggctcgccgt cggagcggag
      961 tcgtcgccct cccccgcccc ccggtacacg tacagcacgt cggcgtccga cccgaacccc
     1021 acctcgcgcc cgccgagccg ccccatggcg acgacgccga actcgaggcc gggcccgccg
     1081 gccacccgca tcgcggtcgc gagcgcaccg gagatgaccg cctcgtcgac atccgcaagc
     1141 cccacaccca gccgggcgac gtcgggagtg cggatgatcg cgttcaacgc gagacgaagc
     1201 cgctcttggc gccgtagcgt gcgcagcgcg gcggccacca tctccgggtc gtcgtaccgt
     1261 cgcaggatcg cgccgacctg gtcatcgagc gcctgccgtg gccgcggcac caacgacgcc
     1321 tcgtcgtcga gccagcccac ggcagagggg atgcgctgga acatgtcggc cacgaagggt
     1381 gcactgctga gcacgcgagt caggttctgg gccgcgcccg aagagtcgcg cagcatgccg
     1441 aggaaccagt gcgtacggcc caggctctcg ctcaggcgac gaaaggccag gagcccgccg
     1501 tccggatcgg cgccctcacc gagccattgc aacaggacgg gcagcaggtt ccgctggatc
     1561 gttgcacggc gcgtcacacc gcggctcaga gcggcgatat gggcgagcgc tgcccgcgtg
     1621 ttccgaaagc cgatcgcctg gagccttacg gccacctgct cgctgtcgag attgaaatcc
     1681 tccggcggcg tcgcggccgc cgcggcgagg agcgggcggt agaagagccg ttcgtgcagg
     1741 tcgcgcaccc gagaacgcac ctccgaccag atcgccgcga ggccctccgg cccctgcgcg
     1801 aggccgctgg agcgcgcgag gattcgcagc ccgtcttcgt cggagggcat gaggtgggtt
     1861 cgtgccaggt gacgcagctg aagccggtgc tccaagagtc gcaaagtgcg atagtcggcc
     1921 gcgagggcgc gggcctcggc acgcccgaca tacccgcgag cggtcaacgc agccaacgcg
     1981 gagagcgtgt cggcctgccg cagatgcgga tcggagcgcc cgtgcaccaa ctgtagaagc
     2041 tgcacggtga attcgatgtc gcgaagcccc ccctcgccaa gcttgatctg ccggtctcgc
     2101 tcgtcgggcg ggatgttccg tatgacccgc cgccgcatct cttgcacccg ggagacgaac
     2161 ccgtcacgct cggcgccgcg ccacacgagc ggcagaaccg cgtcgacgta gcggcgcccg
     2221 agcgcccggt ctccggccaa cggccgggcc ttcaccaggg cctggaactc ccagtccttt
     2281 gcccagcgcc gatagtaggc cgcatgcgac tcgacggtgc gcacgagcgc cccgtctttg
     2341 ccttcggggc gcagattcgt gtcgaccggc cacaaggcgg gctcgtcgga gacctcgctc
     2401 aagcctcgta tcgtctggcg cgcgagacgg gtcgcaatcg tcaccacgcg cgccgtcggc
     2461 accgcgtgtg gccgggcgct ggcgcacgcg aagacgacgt cgacgtcgct gacgtagttc
     2521 agctcgccgg cccctgcctt gcccatgccg atcaccgtca ggcgcgtggc agcgacctcg
     2581 tcggcgggaa agcggcccgg gccctcgccc gcgacgtcgg cgcgggcgag ggcgagggaa
     2641 gcctccagcg ccgccccggc cagctcggcc agcgcacgac cgacggagtc cacgccccgg
     2701 accggcccgg gctgccccaa gtcgtacagc gcgacagtga cgagctcccg ccgataggcc
     2761 acccggaggg cgctccatcc ggtctcaccc gcaaggctcg ccaccccgtg tgcgtcggcg
     2821 ccgaccgcat cgaggaggcg ccggcgcacc ctctccacct cgcccggcac cgcaggcgag
     2881 gaaagttgcg gcatgacctc gaccagggcg cactcgtcgg ggtggcgaaa gagaaactgt
     2941 gccagcccat ccgacgcgcc gacgagtcgg aagagccgcg cggcggaagc ctcgtcgccg
     3001 acgatgcgtc ggatgaggcg cggggcgcgc tcctcgaggc gcagcagcca aacgagcgcc
     3061 gaatccgggt cggccgcgac ccggaagaac ggcagcaggt cgtcggcggc gacctccagc
     3121 cgatcggcga gcgcctcaag ccgggagccc gccgtggaga gcccggcgaa ccccacggcc
     3181 gccaacgcct ggagcccgag ggaccgccgc atgtcagagc atcttgaggt tggagcgcag
     3241 ctcgtagggc gtgacctggg cccggtagtc ctgccagtct tgccgcttgt tgcgcagcac
     3301 gaagttgaag acctgctccc ccagcgtctc ggcgaccaac tccgacttct ccatgatcga
     3361 gatggcgtgg ttgaggttca cgggcagctg ggtgtagccc agggctcggc gctcctcgtc
     3421 gctcaacgcc cacacgttgt tctcggcctc gcgcggcagg tcgaaatggc cttcgacgcc
     3481 cttcaggccg gcggcgatca tcagagagta ggcgagatac gggttcgcgg cggagtcgag
     3541 tgcccggtat tccacccggg aactctgcgg cttgttcggc ttgtagagag gcaccctgac
     3601 gagggccgaa cggttgttgt ggccccaaca cacgaagctc ggcgcctcgt cgccgcccca
     3661 cagcctcttg tatgagttca cgaactggtt ggtcaccgcg gtgatctccg gcgcatagcg
     3721 caagagcccg gcaatgaacc atcggccgac ggcggagagc tggtattggg cgcccggctc
     3781 gaagaaagcg ttgccgtcgc cctcgaaaag ggagaggtgg gtgtgcatgc cgctgccggg
     3841 ctgccccgac agaggcttgg gcatgaaggt cgcgtaagca ccctgctcga tggccacctc
     3901 tttgacgacc gcccggaacg tcatgatgtt gtccgcggtg gcaagcgcgt cggcgtaccg
     3961 caggtcgatc tcgttctggc ccgggccgga ctcgtggtgg ctgaattcga cggagatgcc
     4021 gaggtcttcc aacatgcgca cagagcgccg ccgaaagtcg tgcgccgtgc cgccgggaac
     4081 gttgtcgaag taccccgcgg catccaccgg cgtcgggtgg ccctccgcat ccagcttgga
     4141 ggaggtgagc aggtagaact cgatctcggg atgcgtgtag cacgtgaatc cgagcccggc
     4201 ggctttctcc agcgtgcgtt tcaagacgtt gcgcgggtcg gcgacggccg gctcgccgtc
     4261 gggagtgtag atgtcgcaga acatgcgggc ggtcgggtcc gtctcgccgc gccacggcaa
     4321 gatctggaag gtgcctggat cgggccggag caagacatcc gcctcgtaga gccgtgtgag
     4381 gccttcgacc gcggaaccgt cgatgcccac cccctcggcg aacgcactct ccacctcggc
     4441 gggcgcgatg gcgaccgatt tgagggagcc gatcacgtcg gtgaaccaga ggcgcacgaa
     4501 tttcacgccg cgctcctcca acgtgcgcag tacgaagtca cgctgcttgt ccatgaccgc
     4561 ctctcgtccc agcacagcct agttgttccg ggcgccaaga aggctaccca ggcaggccgc
     4621 ggtcggcatg gcatcacgca ccgcgccgac gggcgccgta ggctccggcg agacccggag
     4681 ccgaggcctc gccgccctgc ggcaccgtag actgacggcc atggccgagt tgacgcctaa
     4741 acgcgtgcgc acccgttact tccgctctgc taaagaacaa ggcacacggc tggtcggcct
     4801 cacaagttat gaccaactga gcgccgcgat cttcgacgcg gctggcatcg atttcctcct
     4861 cgtcggcgac tcggccgcga acaacgtgct cggctatgag acgacgctgc cggcgacggt
     4921 ggacgaactg atccccctcg cgcgcgcggt cgcgggggcg gcgcatcgcg cgctcgtcat
     4981 cgccgacctg ccgttcggct cgtacgaagg cgggccgggt gaggcgctgc gctccgcggt
     5041 gcgattcatg aaggaggcgg gggtcgccgc cgtgaagttg gaaggcggcc gccggcgcgc
     5101 cggcgagatc cgtgagatcg tggatgccgg aatcccggtg ctcgcgcata tcgggttcac
     5161 cccgcagagc gagcacgccc tgggcgggca cgtcatccag ggtcgcggtg cgggtgtcga
     5221 acgccttctc gacgacgccc acgccgtcga ggacgcgggc gccttcgccg tcgtcctcga
     5281 gatggtgccg tcggaggcgg cccggcgggt caccgccgaa ctcacgatct cgacgatcgg
     5341 tgtcggcgcg ggccctgacg tcgacgccca gctgctcgtc tggaccgact gggcgggcct
     5401 caccgtgggt cgcgtgcccc ggttcgtgaa gaagtacgcg gatctcgcga cgacactgac
     5461 cgacgcggtc gccgcctaca gggccgacgt caccgagggg cgctatcccg acgaggagca
     5521 cagctaccgc gagagctgac tcggtctcac aaggcaccca ccgccatgca ccggccccgt
     5581 gccgcggcta gtcttccgcc tcgtcttctt cgcgccagcg acgcgcgttt tcgcgcaaga
     5641 ggtcgggcgc gtgcgtggcc gcgtcgcgcg tcgcgaaggg gccgacccga taggccgagg
     5701 gcgaaagcac gccctcctcc acctcgccgg tgcgtgagtt gtaccagaac tgccgtcgct
     5761 catcggacat gtgccgatcg tacggggctc ggcgcgaaac acgccgcgtc gtcagtgaaa
     5821 gcggcgatac ccgaagatgg agacggacca gacgagaagc aggaagatgc cggcgccgac
     5881 cgcgacccag gccttcgatg tcacatagcc gcctggggtg ggcgaaccga tgttcaggtt
     5941 tccggaggtg gaatcgggtg ccgccgcctg aaccgccacc gccggggtga cgaccttcga
     6001 cgacgtggca gacggcgcct gcgaaaggga gacggcggcg gaggccgagg acacggccgg
     6061 ttcggccacg gcggattgca cgccgacaaa cccgacactg gcgacgatgg ctgctgccgc
     6121 accgaacgct gcgatgttcc tcttcagatg acgcatgcac actcctcact acccagcata
     6181 ttgcctcggg cggccccggc tcgaaggctc gcctcatcaa agcccgcgag gcacgccgac
     6241 actcctcagc ggatgtcctt gacgctggag agccacagcc agagagccga catggcgctc
     6301 acggcgcaga gaacggcgaa gaaggtgagg tgggtgacga tcgccaggac caggaagacg
     6361 agggccgcga tgagaaaacg cacggcggcg ctgcgcatcc gcgggtgacg cgtcttcgat
     6421 gccgacatgg ccctcccatc gtcccgcgag cgtacgcacc acggccgccc gcacgacgcg
     6481 ggggctccgg tcacccaggg tatcctgacc gcgtgcccac tccgcagccg aaccgagacc
     6541 gcagccacaa cccggcctcg tcatcgtgac gacggtacgc ggcacggtgc gctccacggc
     6601 ggattgggag ggcgagggca gcggcccgac ggccgcggcg gcccgcgccg aggcggtgcg
     6661 gggcctccct gacggctttg ctctcgtcga cgtgcgaacc ctcagcgcga agcccggcga
     6721 accggtcacg atgaaggcgt tcgggcgcgg caccgcctct cacgagatcg aggcccgggg
     6781 ggacgacgag cccgaggcgc tcgccgcgct gcgcgaggcg gttccgccgg ggcatctttt
     6841 gttagaggtc gtcatcgtgc ccgacgggac ccgaccgagc gcctgagccc cgacggtcgg
     6901 cacggaatgt cataatcgag gcatggcaat cgtgatgtgg tgcgtcctcg gcgtcttctt
     6961 cgcgctcggc gcgaccgagc tggtcgtcgg cggtgtcgcg ctcgccaagt cgggcaaccg
     7021 ccaagactga ggcaagcctg cgagaccctc cggcaaggcg tcgggaccca gggtcgggcc
     7081 gtgcgtgagg gtgcgactcg tcagacgttc acgtacctcc cggtacggcg agcttcgctt
     7141 gccagccgac ccccggcagc tttccccgag cggttctcag cgactttcag ctacccgacg
     7201 ccaggtgacg aagcggtaac gtaagccggc cgaggagacc tgccaggcgc cccgctcggg
     7261 catgaagcga gcctcgtcga tcttcggtgc ccaggtgtct cccgccacct cgatatccac
     7321 ctccgtcacc tcgatgagcg tggcccaggc catggccgcc gcgtagatct cggcaccacc
     7381 gatgacccag gcccccggcc cgaccgcctc cgccaccgag tgcatgacct tggcaccggg
     7441 ggcgtcgagc gtgcttcggc gggtgaggac gacgttctcg cggcccggca gcgggcgcac
     7501 ggcggccggc aacgactgcc aggtgcgccg ccccatcacg acccggcctc cccacgtgag
     7561 cctacggaac cgccgttggt cttcgggcag gcgccacgga atcctgcccc ccgcgccgat
     7621 cacaccggct aaggtctggg cccagaggag accgacctgg ccgtcgggca ctcggcccgc
     7681 ctgcgccgcc tgggccatcg ggtcagaccg cgacgggcgc gtggatcgcc tcgtggtgcc
     7741 gatacccctc gagaaggaaa tcgtcgtacc gatagtcggc gatgctctcg gcatgacggg
     7801 cgaggctgag cgtcggcgcg ggcagcgcct cccgggtcaa ctgcaggcgc actgcgtcga
     7861 ggtggttgtc gtaaatgtgg cagtccccac ccgtccagac gagttctgcg ggcacgaggc
     7921 cagcctggtc ggcgacgagc accgtcagaa gggcgtagct ggcgatgttg aacggcacgc
     7981 cgagaaacat gtcggcgctg cgctggtaga cctggcacga caagcgcccg tctcgggcat
     8041 agaactggaa gagcacgtgg cagggtgcca acgccatggc gtcgaggtcc gcgacgttcc
     8101 acgccgagac gatcatccgc cgggagtccg ggtcacgacg caacgtgtcg atcacccggg
     8161 cgagctggtc cacgtgccgg ccgtcgggcg tcggccagcg gcgccactgc acgccgtaga
     8221 tcgggccgag atccccgttc tcgtcggccc attcgttcca gatgctgacg ccatgctcct
     8281 gcagccaatg cacgttcgaa tcaccccgca ggaaccacag caactcgtag gcgatcgact
     8341 tgaagtgcac ccgtttcgtc gtgacgagcg ggaagcccgc cgcgagatcg aacctcatct
     8401 gacggccgaa gacgctgcgc gtgcccgtgc ccgtgcggtc gcccttcggc atgccgtgga
     8461 cgagaacgtc gcgcaacagg tcctcgtagg cggtcacgac cgcaggctcg gggctggtca
     8521 cgcaccgatc gtagccgacg ggccagacga tgccgcgccg acgtgccaca cctgcgccgt
     8581 gcggcacgcc gtgtgtcgcg cgtcgtacgg gcggcggtgt cgccgatcac ccacgacgcg
     8641 ccatgcagcc atggggagca gcccgatgcc gagcgcgggc aacagatcgg ctgcgtaggc
     8701 gcaggccaag cgttccgcgc aacccggtgc cgagcggggg caacgccggg tacgcgaacg
     8761 gccggcgcgc gttagcatcg acggatgagc gtcagcctgg ccgagttctg cacgatcgcc
     8821 gatcggctct ggccccccga cggcgccgag gaatgggacg cgcccggcct cgtctgcgga
     8881 gaccccgccg ccgaggtctc ctccgtgttg ctcgcggtgg acgccgtgcc ggctaccgtc
     8941 gaagaggcca cggccgggtc gcacgacctg ctcctcaccc accatccgct gctcttgcgg
     9001 ggcgtgaaga gcatcgccga agaccgttac aagggccggg tgcttgcggc cgcgatacga
     9061 gggggcctcg cgattctcgc cgcccacacg aacgccgaca tcgttcgaag cggcacgagc
     9121 gcagtcctcg ccgcggtgct cgggctggag gacacgcggc cgatcctccc cgcgcacggc
     9181 ccggagccac gggcctcggg gctcggcagg gtggggcgct tgccgtcggc cgaaggcctt
     9241 cggcatctcg cgacgaggct cgcccgcgcc ctgcctgcga cggccggcgg cgtgcgggtc
     9301 gccggggagc cggaggcaca gattcgcgtc gtctccctgt gcgccggagc cggcgactcg
     9361 cttctgtcgg agccggcggt actggccagc gacgtctaca tcacctcgga cctacgccac
     9421 catcccgcct ccgaggcccg cgaacaggct cggctcgccg gcggcccggc gctcatcgac
     9481 gtctcccact gggccgccga gtccctgtgg ctggcgggcg cggcagaaga gctgcggcga
     9541 gccgtgccgg ggctgcgggt cgccgtcagc accctgcgca ccgacccgtg ggacttcacg
     9601 gtggaatcgg gaggtcagcg ccgatgaagg cggcaccgga ggagcagcga cggctcctcg
     9661 acgttcagag gatcgacaac gcgatcacga aggcccgcag cacggcagac gaggcggcgc
     9721 taggcgccga gctggcgggc atcgaggcgg aactgacccg cgcccggcac gagcagctcg
     9781 agagggcggg cgcgttagaa gacggcaaga cggccctcgc acgcatcgag tcggatgtcg
     9841 cggcggtcga cgcgcgcgcc gcgagagaca cagcgcgact gcacgagacc tcctctctca
     9901 aggacatcca cggcctcgag gcggaattgc ggtcgttggc agagcgcaaa gaccgtctgg
     9961 aagacaccca gcttgaggcg atggaggaac tcgacgggct cggccgcgca gcctcggcgg
    10021 ccgagagcct cgtcgacgag acggcccggc ggaaggccga cgccgaagcg cggcgccgga
    10081 catccgctgc cgagcggcag tcacggctcg aagagctctc ggccgaacgc cgcgacgcgc
    10141 tcgtgggcct gcccgaagac ctcgtggcct tgtacgagaa gcaacgcgcc cggtacggca
    10201 tcggcgcggc cctgttacgc ggcggcgtct ctctcggcag taacacgacg ctgaatccgg
    10261 cggacatgca ggacatccgc cgagcggcgc cggacgacgt catcctgtgc ccggagagca
    10321 gctgcattct ggtgcgcacc gaggaatccg gcctgtgagg ccctgctagg ctgggcgccg
    10381 agtgggccgg caaggcggtc gcgtcacgcc gcgaggcgtg ccgaggaacg tccgggctcc
    10441 gcagggcagg acggtgggta acacccaccc ggggtgaccc gcgagacagt gccacagaaa
    10501 acagaccgcc cttcggggta agggtgaaac ggcggtgtaa gagaccacca gcgaccgggg
    10561 tgacccggcc ggctcggcaa acctcgtccg gagcaagatc aacaggagac catggggctg
    10621 cccgtcccgt ctccgggtgg atcgctagag gtgcgcggca acgcgcaccc gagagagatg
    10681 gccgcccaac gctcccctcg gggcgcgtgg acagaacccg gcgtatcagc cggcccgctc
    10741 ctccgccgcc aacgacgcgg ctcccaggat gccggcgttg ttacgcagca cggccggcac
    10801 gatgcgggtc ttcaggttga ggagcggcag gaacgactcg tggtgtttcg acaccccgcc
    10861 gccgacgacg aaaaggtcgg gatcgagcag catctcgagg gtggagtaat accgttgcag
    10921 ccgcctcgcc cacttcgccc agctcaggcc ctcccgcctc tttgccgagt aggccgcccg
    10981 atgttccgcc acatggccgc cgatctccaa gtgcccgagc tccgtgttcg gcaagagcac
    11041 cccggaatgc agcaccgcgc tgccgatacc cgtgccgagc gtcgtcaaga ccgtgacgcc
    11101 ggtgacgccg gcggccgccc cgaaccgggc ctccgcgatg ccggccgcgt cggcgtcgtt
    11161 gatgaaccgc acccggcgac ccaacccgcg ttcgaagagc tcctctgcag cgagcccgat
    11221 ccacaattcg gagacattcg ccgccgacaa ggtgcgcccg gcacgcacga cggcgggaaa
    11281 acagatgcca accggcagac ctggcgccac ttggcgctcg atgatctcca ccacagcgcg
    11341 ggtcacggcg accacgtcgt gtggcctcgc acccttcggg gtggccagct tcactcgctc
    11401 ggtgacgagc cgcccgccgg ccgacagatc gaccgccgcg cccttgatgc ccgtcccccc
    11461 gatgtcgatg ccgaccgcct gcaccgcgct catggcagac accctaccgt ctacctgggc
    11521 ggcgccgggc aggtcgccgg acgaacggcc tcagcggctc tctgacacgc cggggtgcgc
    11581 gtcgcccgcg gggccgccgg cacgcaaccg cgcgatctcg tcgcgctgcc gcgcgatgaa
    11641 gtcgcgcagc tgggcgcgct ccctgatcga gtcgaggtag agctgctgga gcgtcgccag
    11701 acggtcggca ccggtcctcg tcgagggctc cggcaggccc atgacgttct cgccggcaac
    11761 ggcccgcgta cggtcgccgc ggatctcacc cgcccggcgg cgcctcgcga cgccgccatc
    11821 cgactccgcc cgcaccatgc cgccattctc tcaaagccgc gggtgagcgg ccaccggtga
    11881 gcctccggct ccaccccggg cgggcgtcaa ccgccgaggt ggtggggcgt cgcgtacagg
    11941 acccactcgc cgtcgcggaa gagcaccggc ccggcggtca cgacccggcg cgtgtgctcg
    12001 ctcaacccgg tctcgggccg atacaccatg acggcggagc ccgacgtgag atagctcgtg
    12061 acgtgcttct gccaggccag gttcttcgtg cgtggcgtgt agacgccgtc gtgcatgccg
    12121 gtgtcgaggt cgtacgtcca cccggtgacg tcgggccaga gcacacatcc gtcgctgagg
    12181 tcgcgggaga gcacgtcggt tgcggccaga atctcgggat cgtcggagat cacgcagccc
    12241 ggcaccgtgg ccacggcgcg gcgcagcccc gcgctcggat gctccccggg gtgcgcgtgc
    12301 cactgccccg gaaggttcag caacacgagg ccggccaagg ccgtcgtcgt caagactagc
    12361 cggccggacc gggtcgagag gagcccgacg agctcggcga acccggcgcc cagcaccaag
    12421 gccagcggcg ccgacgtcaa cgaggtgtag tagctgaaga acgacggcgc cacgatgagc
    12481 accagcaagc cggaaaggag gagccacaca tagaggcggg ccgagggccg gcggagggcg
    12541 aagatcacgg cgatcaccgc gagcacggcc agccccccgg tgatctcgcc gacggtgagg
    12601 ccgagaagcc tggtcgcggt atgcggcacc gtggctccgc cgaggatgtc gtggaggcgg
    12661 cccaccaccg aaggcccgcc ccggccgcga cccagctggt cgagaacgac ctcacggaac
    12721 atgaccgcgg gtgaggcgag aaagaacggc agcatgatga cgaccgcacc gaccgcctcg
    12781 gcggcaagaa gccgcagccg ccgccgcacg ccgggcgtcg ccgccacgac gaccagggcg
    12841 ggaacgacgt accagatctt gaaaccgcag gcgaggccca aaagcaggcc tgcgacgagc
    12901 atgcggcgac gacctaccgg ccgacgcacg tcgcccaaca acaacagggc gatgagaatg
    12961 agcagggtcg ccacgggctc cagcttgatg aactgctccg acgagaccgc gatatccgtc
    13021 accgcgtaga agacgccgcc gaagaccgcc gccggcaggc cgaaccggcg aagcacggcc
    13081 gccaccatgc cgctgctggc ggcgccgatc cccgcgaaga agaggttcgc cgcgccatag
    13141 cccaacgcgt cgtgcgtctg cgccccgagg aacgcgaacg gggcggtcac gaccgcgacg
    13201 ccgggggtct cgatgaagag aaaatcgcgg tacggcaggc gaccgtggac gagcgcgtcg
    13261 gccgctgcgt agtagacgcc ggagtcgtag ccacccacgt acaccgggcc gccgtgcctc
    13321 aggctgtaga aaacgcgcgc gaggagcccc agaagggcta ctccgacgat cacactcggc
    13381 cacatccacc gcggacgccg acgcctcgtg gtccttcggg gaggggccga ctcgagggcg
    13441 gcaggctccg ccagcgacac cgccatcgtc acgccgcccg tctcgtgccg agcgtgccgt
    13501 ggcacaaggt cacaagacag gcggagggga tgacgacacg gttaccgcga aggctcacga
    13561 cgggaaccat acgaacgggc gccgcagaaa ccgctaagaa accgccgcga cggccaggcg
    13621 ccgacggagg ccaggatcag cgggcgtcgc gcggggaggc gaggggcaga gtcaagacga
    13681 aggtcgtgcc tgccggcgag gtctcggcga cggccacgtt gccgccatag cggccggcga
    13741 tgtctcgcac gagtgccagc cctatgccga aaccacggcg ggccgccgtg cccgacggag
    13801 cctgctggcc gtgcgagaag cggtcgaaga cacgggccgg gtcgacgccg gtgatccccg
    13861 ggccgtggtc ggtcactctc agctcgaaga agcgccgacg ggccggggcc aagacgacct
    13921 cgatggtgcc ggccgaaggg gaatgcttga cggcgttgtc gacgagcacg acgacgcagc
    13981 gacgaaacgt cgcctccggc agggcggcga cgagcccctc tcccccgtcg aggcgcagcg
    14041 tgacatcgtt ctgcccggcg aggaaggcca ggtcgtcgag cacagcccgc acggtgggcc
    14101 tcgccgggca gtcggccctc gtgtccccgt cgacgggcga ggcggccagc agcaggtcgg
    14161 tgatcacctc gccgagagca cgtacgtcgc ggcgcaatcc tgcgaggtcg tcgtgcgggt
    14221 cgcccccggc ctcgagctct tgcgctagaa cctccacgcg cgtcgagatg atcgccaccg
    14281 gcgtgcgcag ttcgtggctg gcgtcggcga cgaaggaacg ctgaatgcgc agcgcctccc
    14341 cgagcggggc gacgacgcgc cgcgcgatga cccagctgag gagccccgcg aggatgacgg
    14401 ccaccacccc cgtcacgacc agggcggtga acatgtcacc cgaggcgatg tagatcttcg
    14461 tctcgccggg tgccggcttc tccaacagtt cgctgggccg agactggtcg aggatgaacg
    14521 cgaccgacac ggccacgatg agcaccacga tcgtgctggc ggcggccgtg atccgcaggg
    14581 cgattgtgcg tgcggcccgc gtcacgacgg cctcgtcgcc ggcgtgcggc tgcgtggcgg
    14641 tcacaacgac cccagccggt atcccttgcg ccgcaccgtc aagatgacgt cgcggtcgat
    14701 cttgccgcgc agataatgca cgtacgtgtc gaccgtgcct ggcttgtcgc cgggcgagaa
    14761 gacacggcgc aggatcgtct cccgcgtgaa cgtcgcgtcc gggttctcgg cgaagacacg
    14821 caagagcgtg ctctcgcgcg gcgtcaggag gatgcgcccg atgtacggcg agacgacgca
    14881 cgcgtcctcc gggtagaaca tccactcccc cacggcgagc ccccggcccc gcggatcgaa
    14941 ccggcgcgtc aacgcgcgaa gacgcgccaa cagctcggcg tattcgaacg gcttgacgag
    15001 ataatcgttg gcacccgcgt cgagcccctc cactttgtcg gcagtggtgc ccaacgccgt
    15061 caacagaagg atgggcgccg tgatatggct ctgacgcagc ttgcgaacga tgctgacgcc
    15121 gtcgaggagc ggcagccgcc ggtcgatcac gagaacctcg tagccgccgc tcagagcgcg
    15181 ttcgaggccg gcctcgccgt cggccacgag atcgaccacg aaggaggatt gcagggcccg
    15241 caggagcagc gggcctaatt ccggatcgtc ttccacgaga aggagcgacg gggatgcctc
    15301 gcccgcctcg gcctctgcca tcggtgatcc cttctcctcg cgcccgcggc gcggccacgc
    15361 cgggct
//
LOCUS       DASTNL010000203        10112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_705630, whole genome shotgun sequence.
ACCESSION   DASTNL010000203 DASTNL010000000
VERSION     DASTNL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705630"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1716
                     /locus_tag="VFQ96_06405"
     CDS             16..1716
                     /locus_tag="VFQ96_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEU0257453.1"
                     /translation="MSLPSDHIDSTRPAPRLPAGQVTLGFRGWVRWFWRQLTSMHTAI
                     FLLLLLAVAALPGSLIPQREADPNGVVQYQAAHPALTPILEKLQFFDVYTSVWFSAIY
                     ILLFISLIGCIIPRVKHHIDALRARPPRTPARLDRMPGYRTESIPAASLRGSQGAGDA
                     IESATEVLRHAGYRTQFYADKHSISVSGERGYLRETGNLVFHIAIMAVLVAVGVGGGY
                     GYSGEKIVMQGQTFVNTLVSYDSFNPGRFFTGERLSPYSLTLKDLAVRYETKNKAAIG
                     QPLDYTAYVTARKPDGSQKADTVKVNTPLYIGGTNVYLLGNGYAPVITVRDPAGHVRY
                     RQPVPFLPQDNNYTSIGVVKIADGLPAQVGIIGSFYPTAGASGGVPVSTFPGLRDPVL
                     SLDVFKGDLGINDGVPRSVYTLDTNAMTQVSGPGVQQPAEQLRVGQTVKLPDGLGSVT
                     FDGVKRYVSFTISHNPAQLWIFICSVFLVGGLLTALLVPRRRMWVKARQQRDGSVLLE
                     YAGLARGDDPGLLAAVESITAAHRTALSGPAGPEPPAGPGGTRPAQPAPAPEVVGEES
                     "
     gene            1838..2866
                     /gene="ccsB"
                     /locus_tag="VFQ96_06410"
     CDS             1838..2866
                     /gene="ccsB"
                     /locus_tag="VFQ96_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEU0257454.1"
                     /translation="MVTAFLSQLSVIVLYASLAVYAAAFISFAMDVAGRAVKVRAASD
                     VTSAVARRAESALAREAGAAGQAGSGHLATMTKTANEVHEHITDVNFSRSQTVGMALT
                     VVALAFELVADVLRGIAAGRVPWSDMYEFSMTGTVLITIVFLIMAKRYNLRFLGVFIT
                     GLVLVLMGISVTRYYVAVVPLPPALQSYWLVIHVLVAMGATGFFALGFALSVAQLMQA
                     RRETQIREKMPVTLRFLETLPGSNVLENLAYRVNITGFILWTFTLIAGAIWAEKAWGN
                     YWGWDTKEVWTFIIWVLYAGFIHARATRGWRGSRSAWLAIVGFGAVMFNFGIVNVFFH
                     GLHSYSGL"
     gene            complement(2941..3957)
                     /locus_tag="VFQ96_06415"
     CDS             complement(2941..3957)
                     /locus_tag="VFQ96_06415"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEU0257455.1"
                     /translation="MSHSSTPPAPSSQAAGPVPSAADLVSRAHVVSLPMVTRFRGITT
                     REALLLEGPVGWSEFSPFVEYDDREAAVWLRAAIDFGWRQQPRPQRERVPVNATVPAL
                     PAAEVAALLSRYPGCRSVKVKVAEPGQSLDDDVARVEAVRREAGQSRIRVDANGRWGV
                     AEAVRAITALSPYGLEYVEQPCATVAELAAVRERVRGLAPIAADESIRKTSDPYAVAR
                     EGAADVIIVKAQPQGGVHQALATIAQVGLPAVVSSAIDTSVGLAMGAWLAAALPRLEH
                     DCGLGTAALLGADVTHLPLVPEDGSIPVRRVTPDAALLEEHQASPERRDWWLDRLRRC
                     AALL"
     gene            4022..4927
                     /locus_tag="VFQ96_06420"
     CDS             4022..4927
                     /locus_tag="VFQ96_06420"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEU0257456.1"
                     /translation="MTERVSEIFDPGAWQDVPGFEALTDVTYHHSLDGRVARVAFDRP
                     EVRNAFRPRTVDQLYATLDDARRDPRIGVVLLTGNGPSPKDGGWAFCSGGDQRVRGRD
                     GYLYAAADGDEAEARVGRLHILEVQRLIRFMPKVVIAVVPGWAAGGGHSLHVVCDLTI
                     ASAEHALFKQTDADVGSFDGGYGSAYFARSVGQKNAREVFFLARTYDARRAYEMGAVN
                     AVVPHESLEATALEWARDILTKSPTSIRMLKFAFNLADDGMAGQQVFAGEATRLAYGT
                     DEAVEGRDAFLEKRSPDWGPFPWQF"
     gene            5011..6381
                     /locus_tag="VFQ96_06425"
     CDS             5011..6381
                     /locus_tag="VFQ96_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0257457.1"
                     /translation="MPQPLMIVPADDPNRVHDALRDALAGGPAVFPRAAGAPAVAGLA
                     AVPGDAAVVVETSGSTSAPKRVVLSRDALLASAAGSDEALSLPSGKRQWLLCLPTHYV
                     AGVQVLVRSIVAGTRPVVDVAGHFDPWRLARAAAALTAPVRLVSVVPVQLARLWRAAV
                     SDPSLAAVVRRFDRILVGGQALETSLFERAVEAGFHVVRTYGSSETAGGCVYDGVPFG
                     AVRARIVDDMVELSGPVLADGYLGDAERTARAFHLETRPGGDAGVPPADGAAGAASAT
                     AGGPVPQGGVPAQTRWYRTGDLGVFSDGRLRVLGRADNVIISGGEKVLLDAVEACVDR
                     LPGLADAVVVGVHDDEWGQVPVVVVASEPGTARGGTEHGQAGDVTAHGAEAPELAAVR
                     GRVAPVLGRQAAPAALVRLDRMPRLASGKPDRRRIARLAAARMRATAAGNAGQADRRM
                     TMEPGR"
     gene            6486..7376
                     /locus_tag="VFQ96_06430"
     CDS             6486..7376
                     /locus_tag="VFQ96_06430"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEU0257458.1"
                     /translation="MKPATWADWVDGARLRTLPLAIAPVLIGTGASVVTLGDQQWHWL
                     RALGCLAVALFLQIAVNFANDYSDGVRGADVYRVGPSRLTGSGKARPRTVLVTALVFF
                     ALAGAVGLALVIVTEHWWLIAVGAAAIVAAWFYTGGKHPYGYHALGEVAVFVFFGLVA
                     TAGTAYILAGQVGFEGWLGGVEAGLLACAVLVANNLRDIDTDRRSGKHTLSVRLGARG
                     SKILFCALVFVAYLIVAFFGLFFTFAWLTLFTLLLAIPACLIVVTGRTSVELVLGLKL
                     ISIAALANGAFLCVAFGVVF"
     gene            complement(7347..7733)
                     /locus_tag="VFQ96_06435"
     CDS             complement(7347..7733)
                     /locus_tag="VFQ96_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HEU0257459.1"
                     /translation="MKGPAVLWYTILRLLLIVVPLAILLVLHVNFIVAVFAATFIGLC
                     LSYILLARLRHRMASGLYERMHRAKPARSEDDDAEDAFVDAALLKEEGAGEPGESATS
                     NATVSTGGAPEAAAANRSEDHAEGDA"
     gene            7776..8168
                     /locus_tag="VFQ96_06440"
     CDS             7776..8168
                     /locus_tag="VFQ96_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEU0257460.1"
                     /translation="MVRLYLLLGVIVAAFYIFSIVDCALRERSRVRVLPKPVWVLIVL
                     IPLIGGALWFLFGRRGRSDGEYRRDTAPDDDPEFLGSLGGDDGAGKDGTAVDENLEER
                     IRELEDELSDLDKKHRDNNGRGAGHPDA"
     gene            8161..9966
                     /gene="menD"
                     /locus_tag="VFQ96_06445"
     CDS             8161..9966
                     /gene="menD"
                     /locus_tag="VFQ96_06445"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEU0257461.1"
                     /translation="MPDASADEISTHPHTADPRREVPVAGLPASPAGAYAAAFLGALF
                     RLGVRHLVVCPGSRSQALALAAAALGRGGAHLHVRIDERDAAFFALGVGIESGRPAVV
                     VTTSGTAAAELHAAVLEAHHAYVPLIVVTADRPAELHGVRANQTTTQTGLYGGALRLE
                     EHVAAPVGAADEGVRAAAVAERALRAALGEATGTPGPVHVNVAFRAPLSGRASAAAAE
                     ERPGDETAGAAAPAVVVAGRRPLTLGLGPRTVVVAGSGAGPDAEELAHEAGWPLLAEV
                     VSGARYGRELVVAYRELLREPSFGGRVERVVVFGHPTLSREVPELLARPDVSVVVVAP
                     HGDDVVGAGVPDARRPTVASAVRVDDTAAGQRDRAWLRQWVTGSRRLVASAGGGGEAP
                     DVARSRSSDPRERAAFARAELAAVREPVTRRLLVETVWQFTWPYDRLILGASRLIREA
                     DRVVPGKKISVYANRGLSGIDGTIATAQGVAAAASDGSQAHTGVTRVLLGDLALLHDA
                     GALLFGSAERRPVMQVIVGNDHGGTIFEGLEVARTASPADFDRVLRTPQDVDLASLAK
                     AYGWEYASAGTYGELERALSGQRAVPRLIEVPLGG"
BASE COUNT         1321 a         3557 c         3623 g         1611 t
ORIGIN      
        1 gggtgatccc gacctatgtc cctgccctct gatcacatcg actccacccg gccggcgccg
       61 cggctgccgg cgggccaggt gacgctcggg ttccgcggct gggtgcgctg gttctggcgt
      121 cagctgacga gcatgcacac ggcgatcttc ctgctcttgc tcttggccgt cgctgcactg
      181 ccgggttcgc tcataccgca gcgggaggcc gacccgaacg gcgtcgtgca gtaccaggcc
      241 gcgcacccgg cgctcacgcc gatcctcgag aagctgcaat tcttcgacgt ctacacgtcg
      301 gtgtggttct cggcgatcta catcctgctc ttcatctcgt tgatcggatg catcatcccg
      361 cgggtgaagc accacatcga cgcgttgcgg gcgaggccgc cgcgcacgcc ggcgaggctc
      421 gaccggatgc ccggctatcg caccgagtcg attcccgccg cgtcgctgcg cgggtcgcaa
      481 ggggccggtg acgcgatcga aagcgcgacc gaggtcttaa gacatgcggg gtatcgcacg
      541 cagttctatg ccgacaagca ctccatctcg gtctctggcg agcgcggcta cctgcgagag
      601 accggcaacc tcgtcttcca catcgcgatc atggcggtac tcgtggcggt cggtgtcggc
      661 ggcgggtacg gctatagcgg agagaagatc gtcatgcagg gccagacgtt cgtcaacacg
      721 ctggtcagct acgactcgtt caacccgggc cggttcttca cgggggaacg gctgagcccc
      781 tactcgctga ccctcaaaga cctcgcggtg cggtatgaga cgaagaacaa ggccgccatc
      841 ggccagccgc tcgactacac cgcatacgtg acggcccgta agcccgacgg ctcgcagaag
      901 gccgacaccg tgaaggtcaa cacgccgctc tacatcggcg gcacgaacgt gtacctgctc
      961 ggcaacgggt acgcgcccgt catcacggtg cgcgacccgg caggccatgt gcggtaccgg
     1021 cagccggtgc cgttcttgcc gcaagacaac aactacacgt cgatcggcgt ggtgaagatc
     1081 gccgacggcc tgcccgcgca ggtgggcatc atcggctcct tctacccgac ggccggcgcg
     1141 agcgggggcg tgcccgtctc gacgttcccg gggctgcgcg accccgtgct gagcctcgat
     1201 gtgttcaagg gcgacctcgg catcaacgac ggcgtgccgc ggtcggtcta cacgctcgac
     1261 acgaacgcca tgacccaggt gtcgggcccc ggcgttcagc agccggcgga acagctgcgc
     1321 gtcgggcaga ccgtgaagct gcctgacggg ctcggcagcg tcaccttcga cggggtgaag
     1381 cgttacgtgt cgttcaccat cagtcacaac cccgcccagt tgtggatctt tatctgctcc
     1441 gtgttcctcg tcggcggcct gctgacggcg ctcctcgtgc cgcgccggcg catgtgggtc
     1501 aaggcgcggc agcagcggga cggcagcgtg ctgctggagt acgcgggcct ggcacgcggc
     1561 gacgacccgg gtctcttggc ggcggtcgaa tcgatcacgg cggcgcaccg cacggcgctt
     1621 tcggggcccg ccgggccgga gccgccagcc gggccgggcg gcacccggcc ggcacagccg
     1681 gccccggcgc cggaggtcgt cggcgaggag tcgtagccgg cggcggatgt ggccggggtc
     1741 ggccgaggcc cggcctggcg ccggcgccgg tcggcgtgac aaaggcagaa ttcctcccgc
     1801 ggcctcgcgc cggagggtga gagagtcgta ggttagcatc gtgaccgcct tcttgtcgca
     1861 gctctccgtg atcgtcctct acgcctcgct tgccgtctac gccgcggcat tcatctcgtt
     1921 cgccatggac gtggcgggcc gcgccgtcaa ggtgcgtgcc gcctccgacg tgacgagcgc
     1981 cgtcgcacgc cgggcggagt cggcgctggc gcgagaggcg ggcgccgcgg gccaggcggg
     2041 cagcggccat cttgcgacca tgacgaagac cgccaacgag gtgcacgagc acatcaccga
     2101 cgtgaacttc tcgcgcagcc agacggtcgg catggcgctg accgtcgtcg cgctcgcctt
     2161 cgagctcgtg gccgacgtgc tgcgcggcat tgccgccggc cgggtgccgt ggtcggatat
     2221 gtacgagttc tcgatgaccg gcaccgtgct catcacgatc gtgttcctca tcatggccaa
     2281 gcgctacaac ctgcgcttcc tcggcgtgtt catcaccggc ctggtgctcg tcttgatggg
     2341 catctccgtc acccggtatt acgtcgccgt cgtgccgctg cccccggcgc tgcagtcgta
     2401 ctggctggtc atccatgtgc tcgtcgcgat gggcgcgacg gggttcttcg ccctcggctt
     2461 cgcactctcc gtcgcccagc tgatgcaggc caggcgcgag acgcagatcc gcgagaagat
     2521 gccggtcacc ctgcggttct tagagacgct gccgggctcg aacgtgctcg agaacctggc
     2581 ctaccgcgtc aacatcaccg ggttcatcct gtggaccttc actctcatcg cgggagcgat
     2641 ctgggccgag aaggcgtggg gcaactattg gggctgggac acgaaagagg tgtggacctt
     2701 catcatctgg gtgctctacg ccggtttcat ccacgcccgc gcgacgcgcg gctggcgggg
     2761 ctcccgctcg gcatggctcg cgatcgtcgg tttcggtgcc gtcatgttca acttcggcat
     2821 cgtgaacgtg ttcttccacg gcctgcactc ctactccggc ctgtgacgcc gtcgccccgg
     2881 tcggtgacgg ggcgcgctgc ccgggcctga cgcccgcccc gtcttcgggg tggcgagcct
     2941 tcagagcagc gcggcgcagc ggcgcagccg gtcgagccac cagtcgcgac gttcgggaga
     3001 ggcctggtgc tcctcgagaa gcgcggcgtc gggcgtcacg cggcgcaccg ggatactgcc
     3061 gtcttcgggc acgagcggca ggtgggtgac gtcggcgccc aggagggcgg ccgtgccgag
     3121 cccgcagtcg tgttccaggc gcggcagggc ggctgccagc cacgcgccca tcgcgagccc
     3181 cacggaggtg tcgatcgcgc tcgagacgac cgccggcagc cccacctggg cgatcgtggc
     3241 gagcgcctgg tgcacgccgc cctgcggctg agccttcacg atgatgacgt cggccgcacc
     3301 ctcgcgggcg accgcgtacg ggtcactcgt cttgcggatg ctctcgtcgg cagcgatcgg
     3361 ggcgaggccg cgcactcgct cacgcaccgc ggccagttcg gcgacggtgg cgcacggctg
     3421 ttcgacgtac tccaggccgt agggcgacag tgcggtgatc gcccgcaccg cctccgccac
     3481 gccccaccgg ccgttcgcat ccacccggat gcggctctgc ccggcctcgc gccgcaccgc
     3541 ctcgacccgg gcgacgtcgt cgtcgagact ctggccgggc tcggcgacct tgaccttgac
     3601 gctgcggcag ccggggtagc ggctaagaag ggccgcgacc tctgcggcag gcagggcggg
     3661 caccgtcgcg ttcaccggca cgcgttcgcg ctgcggccgg ggctgttgcc gccacccgaa
     3721 gtcgatcgcc gcacgcagcc acacggcagc ctctcggtcg tcgtactcga cgaacggcga
     3781 aaattcgctc cacccgacgg ggccttcgag aaggagggcc tcgcgcgtcg tgatgccccg
     3841 gaatcgggtg accatcggca gcgagacgac gtgagcgcgg gagacgaggt cggcggcgga
     3901 ggggacgggc ccggccgcct gcgacgacgg ggcgggcggc gtggagctgt gtgacatacg
     3961 cccagtctgc ccgttcgtcg gcgctgggcg cgttccccgt cgaggtgcca gactgggcgg
     4021 catgaccgaa cgggtttcgg agatcttcga cccgggtgcg tggcaagacg tgcccggctt
     4081 cgaggcgttg accgacgtga cctaccacca cagcctcgac ggccgcgtcg cccgggtcgc
     4141 cttcgaccgc cccgaggtgc gcaacgcgtt ccggccccga accgtcgacc agctgtatgc
     4201 gacgctggac gacgcccggc gcgacccccg catcggcgtc gttctcttga ccggcaacgg
     4261 cccgagcccg aaagacggcg gctgggcgtt ctgctctggc ggcgaccagc gggtgcgcgg
     4321 ccgcgacggc tacctgtatg cggcggccga cggcgacgag gcggaggccc gagtggggcg
     4381 cctgcacatt ctcgaggtgc aacgcctcat ccgcttcatg ccgaaggtcg tcatcgccgt
     4441 cgtgcccggc tgggcggccg gcggcgggca ttccctgcac gtcgtctgcg acctgacgat
     4501 cgcgagcgcc gagcatgctc tcttcaagca gaccgacgcc gatgtcggct cgttcgacgg
     4561 cggctacggc agtgcctatt tcgcccgcag cgtcgggcag aagaacgccc gtgaggtgtt
     4621 ctttctcgcc cgcacctacg acgcccggag ggcgtatgag atgggcgcgg tcaacgcggt
     4681 cgtgccgcac gagagcctcg aggcgacggc gctcgaatgg gcgcgcgaca tcctgaccaa
     4741 gtcgcccacc tccatccgca tgctgaagtt cgccttcaac ctcgccgacg acggcatggc
     4801 cggccagcag gtcttcgcgg gggaggcgac gcgtctggcg tacggcaccg acgaggcggt
     4861 ggagggccgc gacgcgttcc tggagaagcg ttcgcccgac tgggggccgt tcccctggca
     4921 attctgaatc gggcggcagc cggcacggtg cccgcctacg ccggccgccg ctgaacggcc
     4981 gccctcgccc gcgtgcgtaa agttgatggc atgcctcagc cgctcatgat cgtgccggcc
     5041 gacgacccga accgggtgca cgacgcgctc cgcgacgcct tggcgggcgg gcccgcggtc
     5101 ttcccgcggg ccgcgggtgc accggcggtc gccggtcttg ccgccgtgcc gggggacgcg
     5161 gccgtcgtgg tcgagacctc aggttcgacg agcgcgccga aacgggtggt tctctcccgc
     5221 gacgcacttc tcgcctcggc cgccggctcc gacgaggccc tctcgttgcc gtcgggcaag
     5281 cgccaatggc tcctgtgcct gccgacgcac tacgtggcgg gtgttcaggt tctcgtccgt
     5341 tcgatcgtgg cgggtacgcg cccggtcgtc gacgtggccg ggcatttcga cccgtggcgc
     5401 ctcgcccggg cggcggccgc attgaccgct cccgtgcgcc tcgtctccgt cgttccggtg
     5461 cagttggcca ggctctggcg ggccgccgtc tccgacccgt cgctggcggc ggtggtgcgg
     5521 cggttcgacc gcatcctcgt cggcgggcag gcgctggaga cttcactctt cgaacgcgct
     5581 gtggaggccg gcttccacgt ggtgcgcacc tacggctcga gtgagacggc cggcggatgc
     5641 gtgtacgacg gcgtgccctt cggcgcggtg cgggcccgca tcgtcgacga catggtggag
     5701 ctttcggggc cggtgttggc cgacggttat ctcggcgacg cggagcgcac ggcacgggcg
     5761 tttcacctcg agacgcggcc gggcggcgat gcgggcgtcc cccctgcaga cggtgccgcg
     5821 ggcgcggctt cggccacggc cgggggcccg gttccgcagg gcggcgtgcc ggcgcagacc
     5881 cgctggtatc gcacggggga cctcggcgtc ttctccgacg gtcgtctgcg ggtgctgggc
     5941 cgtgccgaca acgtgatcat ctccggcggc gagaaggtgc tgctcgacgc tgtcgaggcc
     6001 tgcgtggatc gcctgccggg cctcgccgac gccgtggtcg tcggcgtgca tgacgacgag
     6061 tggggccagg tgcccgtcgt cgtcgtggcg tcggaacccg gcaccgcccg cggcggcacg
     6121 gaacacggcc aggcaggcga cgtcacggcg catggcgcag aggcgcccga gctcgccgcc
     6181 gtgcgcggcc gggtcgcgcc ggtgctcggg cgccaggcgg cgcccgccgc cctcgtgcgg
     6241 ctcgaccgga tgcccaggct cgcctctggc aaacccgacc gacgccgcat cgcacggctt
     6301 gccgccgccc gcatgcgtgc caccgcggcg gggaacgccg gtcaggccga tcggcgcatg
     6361 acgatggagc cgggccggtg agtgcgaagg ccaggcaggc cgcgggccga aagcgcggca
     6421 cggcgtcgaa gaacggcaaa gccgccaggc gccgctccgg cagcccggtg ccgcctggcg
     6481 cgaaggtgaa gcccgccacc tgggcggact gggtcgacgg ggcgagactg cgcaccctgc
     6541 cgcttgcgat cgcgcccgtg ctgatcggca cgggtgcctc tgtcgtgacc ctcggagacc
     6601 agcagtggca ttggctgcgg gccctcggct gcctcgccgt cgccttgttc ttgcagatcg
     6661 ccgtgaactt cgccaacgac tattccgacg gggtgcgcgg ggccgacgtc taccgggtcg
     6721 gcccctcccg tctcaccggg tcgggcaaag ccaggccgcg caccgtgctc gtgacggcgc
     6781 tcgtcttctt tgcgttggcc ggtgcggtcg gcctggcgct cgtcatcgtc accgagcatt
     6841 ggtggctcat cgccgtcggc gccgccgcga tcgtggccgc gtggttctac accggcggca
     6901 agcatcccta cggctaccat gcgttgggcg aggtcgccgt cttcgtcttc ttcggcctcg
     6961 tcgcgacggc cggcaccgcc tacattctgg ccggccaggt cggtttcgag gggtggctgg
     7021 gcggtgtcga agccgggctg ctcgcctgtg ccgtgctggt cgccaacaac ctgcgcgaca
     7081 tcgacaccga ccggcgcagc ggcaaacaca ccctcagcgt gcggctcggc gcgcgcggct
     7141 ccaagattct cttctgtgcg ctggtgttcg tcgcttacct gatcgtggcg ttctttgggc
     7201 tcttcttcac cttcgcctgg ctcacgctct tcacgctgct cttggcgatc cccgcctgcc
     7261 tcatcgtcgt caccggacgc acctccgtgg agttggtgct ggggttgaag ctgatcagca
     7321 tcgcagcgct cgcgaacggc gcgttcctat gcgtcgcctt cggcgtggtc ttctgaccgg
     7381 ttcgcggcgg cggcctcggg ggcgccgccc gtcgagaccg tcgcgttcga cgtggccgat
     7441 tccccgggct cgcccgcgcc ctcctctttg agtagcgcgg cgtcgacgaa cgcgtcttcg
     7501 gcgtcgtcgt cttcgctgcg tgccggtttc gcgcggtgca tccgctcgta caggccgctc
     7561 gccatccggt ggcgaagccg tgcgagcaga atgtacgaca ggcacaggcc gatgaacgtg
     7621 gccgcgaaga cggcgacgat gaaattgacg tgcagcacga gcaagatcgc cagcggcacc
     7681 acgatgagca gcagccgcag gatcgtgtac cacaggacag ccggaccctt cacgcctccc
     7741 agtgtacgga gagtgcctgg gattaaaatg acggcatggt ccggctctac ctgctgctgg
     7801 gtgtgatcgt cgcggcgttt tacatcttct cgatcgtgga ttgcgcgctg cgcgagcgct
     7861 cccgtgttcg cgtgctgccg aaacccgtgt gggtcttgat cgtgttgatc cccctgatcg
     7921 gcggggcgtt gtggttcctc ttcgggcgcc gcgggcgctc agacggggag tatcgccgtg
     7981 atacggcgcc ggatgacgac cccgagttcc tcggctcgct gggcggtgac gacggcgccg
     8041 gaaaagacgg caccgcggtt gacgagaacc tcgaagaacg catccgagag ttggaagacg
     8101 agctttccga cctcgataag aagcatcgcg acaacaacgg gaggggggcc ggccacccgg
     8161 atgcctgacg cgagcgccga cgagatatcg acccacccgc acaccgccga cccgagaaga
     8221 gaggttcccg tcgccggcct gccggcctcg ccggcaggcg catatgccgc cgccttcctc
     8281 ggcgcgctgt tccggctggg ggtgcgtcac ctcgtcgtct gcccgggctc acgttcgcag
     8341 gcgcttgcgc tcgccgcggc ggcgcttggc cgcggcgggg cgcatcttca cgtgcgcatc
     8401 gatgagcgag acgcggcgtt cttcgcgctc ggcgtcggca tcgagagtgg ccgccccgcg
     8461 gtcgtcgtga cgacgagcgg caccgccgcg gctgagttgc acgccgccgt cttagaggcc
     8521 catcacgcct acgtgccgct tatcgtcgtg acggccgacc ggccggcaga gctgcacggc
     8581 gttcgggcga accagacgac gacccagacc ggcctgtatg gcggggccct gcgcctggag
     8641 gagcatgtcg cggccccggt cggcgctgcc gacgagggcg ttcgggcggc ggccgtggcc
     8701 gagcgggccc ttcgtgcggc gttgggagag gcgaccggca cccccgggcc ggtgcacgtc
     8761 aacgtcgcgt ttcgcgcgcc gctctccggc cgggcctccg cggccgcggc cgaggaacgc
     8821 ccgggtgacg agacagccgg ggcggccgcg cctgccgtcg tggtcgccgg gcggcgaccc
     8881 ctgacgctgg gtctcggccc ccgcaccgtc gtggtggccg gcagcggcgc ggggcctgac
     8941 gcggaagagc tcgcgcacga ggcgggctgg ccgcttctcg ccgaggtcgt gagcggagcg
     9001 cgctacggcc gcgagctggt cgttgcctac cgagaactgc tgcgcgagcc ctcgttcggc
     9061 gggcgcgtgg agagggtcgt cgtcttcggg catccgaccc tgagccgcga ggttccagag
     9121 ctgctcgcga gacccgacgt gtccgtcgtc gtcgtcgccc cgcacggcga cgacgtcgtg
     9181 ggtgccgggg tgcccgacgc ccggcgaccg accgtcgcct ccgccgtgcg cgtcgacgac
     9241 accgccgccg ggcagcggga tcgcgcgtgg ctgcgtcagt gggtcactgg atctcggcgc
     9301 ctcgttgcgt cggccggcgg cgggggcgag gcgcccgacg tggcacgttc acgctcttcc
     9361 gacccgcgcg agcgtgccgc gttcgcccgc gccgagctcg cggcggtgcg ggagcccgtg
     9421 accaggcgcc tgctcgtgga gaccgtgtgg cagttcacct ggccgtacga ccggctgatc
     9481 ctcggagcct cccgcctcat ccgcgaggcc gacagggtgg tgccgggcaa gaagatctcg
     9541 gtctatgcga accggggtct ctcgggcatc gacggcacga tcgcgacggc ccagggtgtc
     9601 gccgccgcgg cctccgacgg ctcgcaggcg cataccggcg tgacccgcgt gctcttgggc
     9661 gacctcgccc ttctgcacga cgcgggcgcg ctgctcttcg gcagcgccga acgccgcccg
     9721 gtcatgcagg tgatcgtcgg caacgatcac ggcggcacca tcttcgaagg tctcgaggtc
     9781 gcgcgcacgg cgtcgcccgc cgacttcgac cgggtgctgc gcacgccgca agacgtggac
     9841 ctcgcctcgc tcgcgaaggc ctacggctgg gaatatgcct ccgccggcac ctacggcgaa
     9901 ctggagcggg cgctctccgg tcagcgtgcc gtgccgcgcc tcatcgaggt gccgctcggc
     9961 ggttgagtct ccgggcgccg gagggactgt gcaccgaagc ccgagcaccg gagtccgagc
    10021 gtcggagccg ccggatggtc gcggcccggt cgcaccacgc tgtcggtggg cgtgtgctgc
    10081 ggccggccgg tcagccgaac gtttcgacga tg
//
LOCUS       DASTNL010000204         9623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_890809, whole genome shotgun sequence.
ACCESSION   DASTNL010000204 DASTNL010000000
VERSION     DASTNL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890809"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFQ96_06450"
     CDS             <1..879
                     /locus_tag="VFQ96_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU0257462.1"
                     /translation="IRQVSEAVGSNGSTSMGSVCASTLSLLNAGVPLRAPVAGIAMGL
                     VSDTVNGETRYATLTDILGAEDALGDMDFKVAGTSEFVTAIQLDTKLDGIPSSVLDGA
                     LGQAKEARLTILDVLNRAIDRPDEMAPTAPRVLSVQIPVDKIGELIGPKGKTINGIQD
                     ETGADISIEEDGTVYIGATDGPAAEAARDRVNAIANPTNPEVGEQFLGTVVKIATFGA
                     FISLLPGRDGLLHISEVRKLVGGKRIHDVDEVLSVGQKILVEITKVDDRGKLSLAPVL
                     PEDEAVTAEAGEVTVR"
     gene            1181..2197
                     /locus_tag="VFQ96_06455"
     CDS             1181..2197
                     /locus_tag="VFQ96_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0257463.1"
                     /translation="MHSHPHPAPRVGADVEDIDGRIEPTGSLSILAPRRFGHTGLAAL
                     PAALRVTPAKTAPGGLESLLNRFRGLGGTVFDTSDETAADRAAQLRLGRWRVADDAIV
                     TLRTGRNPGPGGLSPVALRQAVEATLTRLRRERIDVLYLHGEDGMTPLAESLAAAERL
                     IDSGKVGSLGAAQVSAARLIEARVLASTGLPKLLAVQCDYGILHRRHYETELRIVCAG
                     QELSVSPTDPWPAPPAGSAGRGWQARAFEVAGGVPGWAGLVAGHRARIASALGHVADE
                     QGVAPAVVALAWLLAQRGIQAPVVAATSLRHVDAMMAACAIRLSRTHLRELGTASAWR
                     GMAR"
     gene            2262..3617
                     /locus_tag="VFQ96_06460"
     CDS             2262..3617
                     /locus_tag="VFQ96_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0257464.1"
                     /translation="MNGAVDFPLGQADLAFTASGGARVRRTVLPGGIRVLSENVPGAR
                     TATIGYWVAVGSRDEKSPGARPSAEASYGSTHFLEHLLFKGTTRRSALDIAVAFDTVG
                     GEHNALTAKEFTCYYAKVLDRDLPMAVEVIADMLTSSVLDEGEFETERGVILEELSMA
                     QDDPADVVNERIFEAVLGEHPLGRPVGGSPDDIRGVGRDAVWRHYRTNYRPQDLVVTA
                     AGALDHDALVAGVERAVTAAGWNLGVPASPAPRRPGVPAPVGPGRTIVLSRPIEQANL
                     IVGMPGILATDERRFVMGVLGAVLGGGMSSRLFQEVREKRGLAYAVYSFSAAFSDAGV
                     FGLYAGCSPERAREVATLMLDELHRLADHGITDEELRRAVGQLSGGAALALEDSDTRM
                     SRLGRAEITMGQFADLDENLRRLSEVTPADVQSLARDLAARPVSVAAVGAMDDDLKGL
                     G"
     gene            3626..4228
                     /locus_tag="VFQ96_06465"
     CDS             3626..4228
                     /locus_tag="VFQ96_06465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0257465.1"
                     /translation="MSHYVYLVRHGEQQDAEHGLPDGPLSARGIRQATRIAERLSGVP
                     FDGMFHSPLRRAAETAHIRHERMPALEPQPSALLFDCIPSGPGPDLPRAFEPFFGAVT
                     KEEIEAGRAQMTDAVDAFLTPSGEDRHTLLITHNFVIGWFVRHVFDAPEWRWLGVNQA
                     HCGLTIIRVRTAKPAVLVTHNDLAHLPVELRTGLPDAQPV"
     gene            4253..5017
                     /gene="dapB"
                     /locus_tag="VFQ96_06470"
     CDS             4253..5017
                     /gene="dapB"
                     /locus_tag="VFQ96_06470"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU0257466.1"
                     /translation="MGIRVAVAGATGKLGSVAARLVEEHDDLELAAALDSTTPWRAME
                     GAQVLFDATLPAVSPRLVEAALAAGLDVVVATSGWSAERLAGLEARLPAGRRVIVVPN
                     FSLGSVLATEFAARAALFYESVEIVETHRAGKVDSPSGTAVRTAELIAAARAGLGPTE
                     APHADQRARGQQVAGVPVHSLRMAGVVARQDVYFGGPGERLTITHDTADSRAYEAGIL
                     LALRSLHRLTGLTVGLASLLASSPGEAGGGRAGGAA"
     gene            5041..5478
                     /locus_tag="VFQ96_06475"
     CDS             5041..5478
                     /locus_tag="VFQ96_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257467.1"
                     /translation="MAALLLLYIVVAGQRAVLLFASGDAVGIAMGIALGVLPLIALWL
                     LWRELSFGLRSQRLVRLLEAQGALPVDDMPHRASGRPLRDAADLQFPRYREEAERHPE
                     AWQSWFRLGLAYDACGDRRRARAAVRHAIDLERGRRPRSAASQ"
     gene            complement(5535..6443)
                     /locus_tag="VFQ96_06480"
     CDS             complement(5535..6443)
                     /locus_tag="VFQ96_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU0257468.1"
                     /translation="MKILLTGASGLIGSRLTPHLQSLGHEVVRLVRRAPRGPDEHNWA
                     PAAHMLDAALLGDVDAVVNLSGASLARLPWTRAYREKLRQSRLAATRTVTDAMALAGN
                     APRILVNASAVGYYGDRPGLRLTEQAGRGSGFLADLAADWEEAARLAPAGTRVVLLRT
                     GLVVAAGGAVKPLAFLARAGLGGPIGTGGQHWPWIGLEDEVRAIAHLVEGDLEGPVNL
                     AGPTPATADEMLRALAARLGRPYRLRLGTGLIGGLLGDAGRELLLASQKVVPARLLAD
                     GFSFAAPTVAEALAAAFEADGPARDA"
     gene            6560..7537
                     /gene="dapA"
                     /locus_tag="VFQ96_06485"
     CDS             6560..7537
                     /gene="dapA"
                     /locus_tag="VFQ96_06485"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU0257469.1"
                     /translation="MANVDNPFGQVLVALVTPMTADGEVDWAGVEKHIDDVILGGADG
                     ILVSGTTGETSTLTDAEKIRLVEVGKSVAAGRAKILIGGCSNETAHAIQLAKACEKAG
                     ADANLVVTPYYNKPTQAGILTHFRMIADATDLPMILYDIPGRTGVPIAFETLLRLAQH
                     PNILGVKDAKGDFSQVSRVLNQTDLLYFSGADENVLPHLAIGASGLIGVTDNIAPRPY
                     RAIVDAVNAGDLATATAWHKRLEPLVRAVMTHVPGTVSAKYILHGLGRIQSPRVRLPL
                     VGPEEAEAALIEAEIADLAGIPGVDFSDFRPDRNAAAGGALPRVAGTTR"
     gene            7640..9313
                     /locus_tag="VFQ96_06490"
     CDS             7640..9313
                     /locus_tag="VFQ96_06490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU0257470.1"
                     /translation="MSPLSEPAPLQPGTLRIIPTGGLGEIGRNMTVFEIDGKILIVDC
                     GVLFPEEDQPGVDLILPDFAPVRDRLDDVVGVVLTHGHEDHIGAVPYLLNLRADIPLI
                     GSGLTLALVEAKLKEHHIKPYSLTVREGQKEELGPFSLEFVAVNHSIPDALAVAITTK
                     AGVVLHTGDFKMDQLPLDGRLTDLRAFARLGEAGVDLFMVDSTNADVPGFIAGERTIG
                     PVLDNVIARAPRRVIVASFSSHVHRVQQVLDASRANGRRVALLGRSMVRNMTIAADLG
                     YLKVPEGVLIDFKKAGDLPDDKIVYMSTGSQGEPMAVLARMANLDHRIQPGPGDTVIL
                     ASSLIPGNENAVYRVIDGLTKLGATVVHKGNAKVHVSGHAASGELLYCYNIVKPRGVL
                     PVHGEYRHLVANAALARQAGVPEENTIVAEDGAVIDVKDGVARVVGQVDVGYVYVDGA
                     SVGEITDADLKDRRILGEEGFISIIVVVDQATGHIVSGPEIHARGFAEDDAVFDAIKP
                     KIASALAAAVQSGVHDQHALSQTVRRTVGRWVSNSYRRRPMIIPLVIGA"
     gene            9399..>9623
                     /locus_tag="VFQ96_06495"
     CDS             9399..>9623
                     /locus_tag="VFQ96_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257471.1"
                     /translation="MPRRRDGAGKAGKTRQSAAARRSGTPAGGGSKKAARPRTTPRAK
                     TTSTAAGARGKARQGSAPRSRQTAQTLPYPS"
BASE COUNT         1379 a         3483 c         3366 g         1395 t
ORIGIN      
        1 atccggcagg tctccgaggc ggtcggctcg aacggttcca cctcgatggg ctccgtctgc
       61 gcgtcgacgc tctcgctcct gaacgcgggc gtgccgctgc gcgcaccggt cgcgggcatc
      121 gcgatgggcc tcgtctccga caccgtcaac ggtgagacgc gctatgccac cctcaccgac
      181 attctgggtg ccgaagacgc cttgggcgac atggacttca aggtggccgg cacgagcgag
      241 ttcgtcacgg ccatccagct ggacacgaag ctcgacggca tcccctccag cgtgctcgac
      301 ggggcgctcg gccaggccaa agaggccagg ctgacgattc tcgacgtttt gaaccgggcc
      361 atcgaccggc ccgacgagat ggcgccgacc gcgccgcggg tgttgagcgt gcagatcccc
      421 gtcgacaaga tcggcgagct gatcggcccg aagggcaaga cgatcaacgg catccaagac
      481 gagaccgggg ccgatatctc gatcgaggag gacggcaccg tatacatcgg cgcgaccgac
      541 ggccctgccg ccgaggccgc ccgtgaccgg gtgaacgcca tcgccaaccc gaccaacccg
      601 gaggtggggg agcagttcct cggtaccgtc gtgaagatcg ccacattcgg cgccttcatc
      661 tcgctcctgc cggggcgcga cggccttctg cacatctccg aggtgcgtaa gctcgtcggc
      721 ggcaagcgga tccatgacgt cgacgaggtg ctgagcgtcg gccagaagat cctcgtcgag
      781 atcacgaagg tggacgaccg cggcaagctc tccctcgcgc ccgtccttcc cgaggacgag
      841 gcggtcacgg cggaggccgg cgaggtcacc gtccggtaac cgcccgcggc gattttccgg
      901 cggcccggga ccgttcgcgg tcctgggccg cgctgtgctt cggcactccc ctatcgcttg
      961 aaagcgcgct gcggacacga ttgcataacg ttcggcattg ttccgcggcg ggcgaatcgg
     1021 ccggtgtcgc acgccgctta acctgaaaag gtgaaagccc ggcacgggct gatgccgcgc
     1081 tcctgcggcc cggcggccgg gggaggggag cgggccggtg tctttgagcc cgccacggca
     1141 tgccggagag gccggcaggg cgtcgcgcgc acacccgcgc gtgcactccc acccacaccc
     1201 ggcaccccgg gtgggcgccg atgtggaaga catcgacggt cggatcgagc ccaccggctc
     1261 tctttctatc ctcgcgccgc gccggttcgg tcacaccggt ctcgcggcgt tgcccgccgc
     1321 attgcgggtg accccggcga agaccgcgcc ggggggcctc gaatcactcc tcaaccgttt
     1381 ccgcggcctg ggcggcaccg ttttcgacac ctccgacgag actgccgccg accgcgcggc
     1441 gcagctccgg ctgggccggt ggcgggtggc cgacgacgcc atcgtcacgc tgcgcaccgg
     1501 ccggaaccca gggcccggcg ggctcagccc cgtggcgctc cggcaggccg tggaggcgac
     1561 gctgacgagg cttcgccgtg aacgcatcga cgtgctgtac ctgcacggcg aagacggcat
     1621 gacgcccctg gcagagagcc tcgccgcggc ggaacggctg atcgattccg gcaaagtggg
     1681 cagcctcggc gccgcgcagg tgagcgcggc acggctgatc gaggcgcgtg tcttggcctc
     1741 taccgggctg ccgaagcttc tcgccgtgca atgcgactac ggcatcctgc atcggcgcca
     1801 ctacgagacc gagttgcgca tcgtctgcgc cggccaagag ctctcggtca gcccgaccga
     1861 tccttggccc gccccgccgg cgggcagcgc cggccgcggg tggcaggcca gggcattcga
     1921 ggtcgcgggc ggcgtgcccg gctgggccgg gctcgtcgcc gggcaccgcg cccgcatcgc
     1981 atcggcgctc ggccacgtcg ccgacgagca aggcgtcgct ccggccgtcg tcgcgcttgc
     2041 ctggctcttg gcgcagcgcg ggatccaggc gcccgtcgtc gccgcgacga gcctgcgtca
     2101 cgtcgacgcg atgatggcgg cgtgcgcgat caggctgagc cgtacccacc tgcgggagct
     2161 cggcaccgcc tccgcctggc gaggcatggc gcggtagggg cggctgcggc gcgcccgcgg
     2221 gggcctggcc gcgcggatcc cccggcgtag ggtggggggt gatgaacggc gcagttgact
     2281 ttcccctcgg ccaagccgac ctcgccttca ccgcatccgg cggggcgaga gtgcgccgca
     2341 cggttctgcc gggcggcatc cgcgtgctga gcgaaaacgt gcccggggcc cgcaccgcga
     2401 cgatcggtta ctgggtggcg gtcggctcgc gcgacgagaa gagccccggg gcaaggccgt
     2461 ccgccgaggc cagctacggc tcgacccact ttctcgagca cctgctcttc aagggcacca
     2521 cccggcgtag cgcgctcgac atcgccgtgg cattcgacac ggtcggcggg gagcacaacg
     2581 cgctcaccgc gaaagagttc acctgctact acgccaaagt tctcgaccgc gacctgccga
     2641 tggcggtcga ggtcatcgcc gacatgctca cctcgtccgt tctcgacgag ggcgagttcg
     2701 agactgagcg cggcgtgatc ctcgaagagc tttccatggc gcaagacgac ccggccgacg
     2761 tcgtcaacga gcgtattttc gaggcggttc tgggcgagca cccgctcggc cggccggtgg
     2821 gcggcagccc cgacgatatc cgcggcgtcg gccgcgatgc cgtgtggcgg cattaccgca
     2881 cgaactaccg gccacaggat ctcgtcgtca ccgcggcggg ggcactcgac catgacgcgc
     2941 tcgtggccgg tgtggaacgc gccgtcacgg ccgccggctg gaacctcggc gtgcccgcct
     3001 cgccggcgcc acggcgcccc ggcgttcccg ccccggtcgg tccgggccgt acgatcgtac
     3061 tttcccgccc gatcgagcag gcgaacctca tcgtcggcat gccgggcatc ctcgccacag
     3121 atgagcgtcg tttcgtcatg ggggtgctgg gcgcggtcct cggcggcggc atgtcgagca
     3181 ggctctttca agaggtgcgc gagaagcggg gcctggccta cgctgtctac tcgttctcgg
     3241 cggcgttctc cgacgccggc gtcttcgggc tgtatgccgg ctgctcgccg gaacgcgccc
     3301 gcgaggtggc tactttgatg ctcgacgagc ttcatcgtct cgccgaccac ggcatcaccg
     3361 acgaggagct gcgccgcgcc gtcggccagc tctccggcgg ggcggcgctc gccctggagg
     3421 attccgacac gaggatgtcg aggctcggcc gtgccgagat cacgatgggc cagttcgccg
     3481 acctcgacga gaacctacgg cggctttccg aggtgacgcc ggccgacgtg cagagcctcg
     3541 cgagggatct cgccgcccgc ccggtctcgg tcgcggcggt cggggccatg gacgacgacc
     3601 tcaagggcct tggctaggag aagccatgtc gcattacgtg tatctcgtcc ggcacggcga
     3661 gcagcaggac gccgagcacg gcctgcccga cgggccgctc tccgcccgcg gcatccgcca
     3721 ggccacccgc attgccgagc ggctgagtgg agtgccgttc gacggcatgt tccattcgcc
     3781 gctgcgccgc gcggccgaga cggcgcacat caggcacgag cggatgccgg cgctcgagcc
     3841 gcagccgtcg gccttgctgt tcgactgcat accctcgggc cctggtcccg atctgccgcg
     3901 tgcgttcgag ccgttcttcg gcgccgtgac gaaagaagag atcgaggcgg gccgtgcgca
     3961 gatgaccgac gcggtcgacg ccttcctcac cccgtccggc gaagaccggc acacgctgct
     4021 cataacccac aacttcgtga tcggctggtt cgtccggcac gtcttcgacg cgccggaatg
     4081 gcggtggctg ggcgtgaatc aggcgcactg cgggctgacg atcatccggg tgcgcaccgc
     4141 gaagcccgcc gtactcgtca ctcataacga tctcgcgcac ctgccggtcg agttgcgcac
     4201 ggggttgccc gatgcgcagc ccgtctgagg ccggcccggt acattgacgg ctatgggcat
     4261 acgagtggcg gtggccggtg ccaccggcaa gctggggagc gtcgcggcgc gcctggtcga
     4321 agagcacgac gacctggagt tggcggcggc cctcgactcc acgactccgt ggcgggcgat
     4381 ggagggggcg caggttcttt tcgacgcgac gttgcccgcg gtcagcccgc gcctcgtcga
     4441 ggccgccttg gcggccgggc tcgacgtcgt cgtcgcgacc tcgggctggt cggccgaacg
     4501 cctcgccggg ctcgaggcgc ggctgccggc cggccgccgc gtcatcgtcg tgccgaactt
     4561 ctcgctcggc tccgtgctcg ccaccgagtt cgcggcccgc gcggcgctgt tctacgagtc
     4621 ggtggagatc gtcgagacgc accgtgcggg caaggtcgac tcaccgtcag gcacggcggt
     4681 gcgcaccgcc gagctcatcg cggccgcccg ggccggcctc ggcccgaccg aggcgccgca
     4741 cgccgaccag cgggcccgcg gccagcaggt cgccggggtt ccggtgcaca gcctgcggat
     4801 ggcgggcgtc gtcgcccggc aagacgtcta tttcggcggg cctggcgaac ggctgacgat
     4861 cactcacgac acggccgact cgcgggcgta tgaggcggga atcctcttgg cgttgcggag
     4921 ccttcaccgc ctgaccgggc tgaccgtcgg gcttgcctcc cttcttgcct cgtcgcccgg
     4981 cgaggccggc ggcgggcggg cgggcggtgc cgcgtgaaga atcgtctcgc cgcgctcttc
     5041 atggcggcgc tcttgctgct ctacatcgtg gtagccggcc aacgggccgt gctgctcttc
     5101 gcctcgggcg atgcggtcgg catcgccatg ggtatcgcgt tgggggtctt gccgctcatc
     5161 gctttgtggc tcctgtggcg ggagctctcc ttcgggttgc gctcgcagcg ccttgtgcgc
     5221 ctcctcgaag cgcaaggcgc gctgccggtc gacgacatgc cgcaccgggc cagcggccgc
     5281 ccgctgcgcg acgcggccga cctgcagttc ccccgctacc gggaggaggc agagcggcat
     5341 cccgaggcgt ggcagagctg gttccgcctg ggcctcgcct acgacgcgtg cggcgaccgg
     5401 cgtcgggccc gcgcggcggt gcgtcatgcc atcgacctgg agcgcgggcg ccggccccgc
     5461 tccgccgcgt cgcagtaggc ccggcggcgg gccccggcgg tcggtacgac ggcctctcgc
     5521 gccggcccgc tgcctcaggc gtctcgcgcc ggcccgtccg cctcgaatgc ggcggcgagc
     5581 gcctcggcca ccgtcggcgc ggcgaacgag aacccgtcgg cgaggagtcg tgccggcacg
     5641 accttttggc tcgccaggag cagctcccgg ccggcgtcgc ccagaaggcc gccgatgagg
     5701 cccgtgccca gccgcagccg gtaagggcgc ccgaggcgcg ccgcgagggc ccgcaacatc
     5761 tcgtcggcgg tcgccggggt gggccccgcc aggttcaccg ggccttcgag atcgccctcc
     5821 acgaggtgcg cgatcgcccg cacctcgtct tccaggccga tccacggcca atgctgcccg
     5881 ccggtgccga tcggcccgcc gagccccgcc cgggcgagga acgccagcgg cttcacagcc
     5941 ccgcctgccg cgacgacgag cccggtgcgc agcaacacca cccgggtgcc cgccggggcg
     6001 aggcgggcgg cctcttccca atcggcggcg aggtctgcga gaaagcccga gcctcggccg
     6061 gcctgctcgg tgaggcgcag gcccggccgg tcgccgtagt acccgaccgc cgacgcgttt
     6121 acgaggatcc ggggcgcatt gccggccagc gccatggcgt ccgtcacggt gcgggtcgcc
     6181 gccagccgcg actggcgaag cttctcccgg tatgcccggg tccacggcaa tcgggcgagg
     6241 ctcgcgccgg agagattcac gacggcgtcg acgtcgccca ggagcgccgc atccagcatg
     6301 tgtgcggcgg gggcccagtt gtgctcgtcc ggcccgcggg gcgcgcgacg cacgagccgc
     6361 accacctcgt ggccgaggct ttgcaggtgt ggcgtcagcc ggctgccgat cagtcccgag
     6421 gcgccggtga gaagaatctt caccgcttgc ggcgaagcgt cgcccgcgtc tcgttcatgc
     6481 cgcctcccgg ggcattgatc ctactcgtcg acagcatggc gccgtgggcc gggcgcggcg
     6541 cggacggtac cctggacggg tggctaacgt tgacaacccc ttcgggcagg tgctcgtcgc
     6601 gctcgtgacg ccgatgacgg ccgacggcga ggtcgactgg gcgggtgtcg agaagcatat
     6661 cgacgacgtc atcctgggcg gcgccgacgg catcctcgtc tccggcacca cgggcgagac
     6721 ctcgaccctg accgacgcgg agaagatccg gctcgtcgag gtcggcaagt cggtcgccgc
     6781 cggccgcgcc aagatcctca tcggcgggtg ctcgaacgag acggcgcacg cgatccagct
     6841 cgcgaaggcg tgcgagaagg cgggggccga cgcgaatctc gtcgtcacgc cctattacaa
     6901 caagccgacg caggccggaa tcttgacgca ctttcgcatg atcgccgacg ccaccgacct
     6961 tccgatgatc ctgtacgaca tcccgggccg cacgggcgtg ccgatcgcct tcgagacctt
     7021 gttgcgcctc gcccagcacc cgaacatcct cggcgtgaaa gacgccaagg gcgacttcag
     7081 ccaggtctct cgggtcttga accagacgga tctgctgtac ttctccggcg ccgacgagaa
     7141 cgtgctgccg catctggcga tcggggcgag cgggctgatc ggcgtcaccg acaacattgc
     7201 gccgaggccc taccgcgcca tcgtcgacgc cgtcaacgcc ggcgatctcg cgaccgcgac
     7261 cgcgtggcac aagcggctcg agcctctcgt gcgcgccgtc atgacacacg tgccgggcac
     7321 cgtgtcggcc aagtacatcc tgcacggcct ggggcgcatc cagagccccc gggtgcggct
     7381 gcccctcgtg ggcccggaag aggcggaggc ggcgctcatc gaggcggaga tcgccgacct
     7441 ggccggcatt ccgggcgtcg acttcagtga tttccgtccc gaccggaacg cggccgcggg
     7501 cggtgccctt ccgagggtcg ccggcaccac gcggtgaccg accagggcta gggcggcccg
     7561 cgtcggccgc cccggcccat cacacgcggg caggcagagc cccggcccgc ggcatcccga
     7621 cgagaggacc ttttaccgta tgtcgcccct gagtgaaccc gcaccgttac aacccgggac
     7681 cttgcgcatc atccccacgg gaggtctcgg cgagatcggc cggaacatga cggtgttcga
     7741 gatcgacggc aagatcctca tcgtcgactg cggtgtgctc ttccccgagg aggatcagcc
     7801 gggcgtcgac ctcatcctgc ccgacttcgc ccccgtccgc gaccggctgg acgacgtcgt
     7861 cggcgtcgtc ctgacccacg gccacgagga tcacatcggc gccgtgccat accttttgaa
     7921 cctgcgcgcc gacatcccgc tgatcggctc gggcctgacc ctggcgctcg tcgaggcgaa
     7981 gctcaaagag caccacatca agccgtacag cctgacggtg cgcgagggcc agaaagaaga
     8041 gctggggccg ttctctctcg agttcgtcgc ggtcaaccat tcgattcccg acgcgctcgc
     8101 cgtcgcgatc acgacgaagg ccggcgtggt gctgcacacc ggcgacttca agatggacca
     8161 gctgccgctt gacggaaggc tcaccgacct gcgtgccttc gcgaggctcg gcgaggccgg
     8221 tgtcgacctt ttcatggtcg attccaccaa cgccgacgtc ccgggcttca tcgccggcga
     8281 gcgcacgatc ggcccggtgc tcgacaacgt gatcgcccgc gccccgcgcc gtgtcatcgt
     8341 cgccagcttc tcgagccacg tgcaccgggt gcagcaggtt ctcgacgcct cccgggccaa
     8401 cggccgccgc gtcgcgctcc tcggccgttc catggtgcgc aacatgacga tcgccgccga
     8461 cctcggctac ctgaaggtgc cggagggcgt gctcatcgac ttcaagaaag cgggcgacct
     8521 gcccgacgac aagatcgtct acatgtcgac gggctcgcag ggcgagccga tggctgtgct
     8581 cgcccgcatg gcgaatctcg accaccgcat ccagcccggc cccggcgaca ccgtcatcct
     8641 cgcctcgagc ctcatccccg gcaacgagaa cgcggtgtac cgcgtcatcg acggcctgac
     8701 caagctgggg gcgaccgtcg tgcacaaggg caacgcgaag gtccacgtct cggggcatgc
     8761 cgcctcgggc gaactgctgt actgctacaa catcgtgaag ccgcgcggcg tcctgcccgt
     8821 gcacggcgag tatcggcatc tggtcgccaa cgccgccctc gcccggcagg cgggggtccc
     8881 ggaggagaac acgatcgtcg ccgaagacgg cgcggtcatc gacgtgaagg acggcgtcgc
     8941 ccgggtcgtc ggccaggtgg acgtcggcta cgtctacgtc gacggcgcga gtgtcggcga
     9001 gatcaccgac gccgacctga aggaccggcg catccttggc gaggagggct tcatctccat
     9061 catcgtcgtc gtcgaccagg cgaccggcca catcgtctcc ggcccggaga tccacgcgcg
     9121 tggtttcgcc gaggacgacg ccgtcttcga cgccatcaag cccaagatcg cttcggccct
     9181 cgccgccgcc gtgcaaagcg gcgtgcacga ccagcacgca ctcagccaga cggtgcggcg
     9241 caccgtgggc cgctgggtct cgaacagcta ccggcgccgc ccgatgatca tcccactcgt
     9301 catcggcgcc tgagcctccc agcgccttgc gccccgctcc ggccaaacgc ccggcgggct
     9361 taccggctcg cgggccgtcg aacggtagcc tggaggctat gcccaggcgc agggacgggg
     9421 ccggcaaggc cggcaagacg cggcagagcg ccgcggcgag gcgttccggc acgcccgcgg
     9481 gcggcgggtc gaagaaggcg gcgcggcccc gcacgacgcc ccgggcgaag acgacgagca
     9541 ctgcggcggg ggcccgcggc aaggcgcggc agggttctgc gccccgcagc aggcagacgg
     9601 cacagactct gccgtacccg agt
//
LOCUS       DASTNL010000205         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1075500, whole genome shotgun sequence.
ACCESSION   DASTNL010000205 DASTNL010000000
VERSION     DASTNL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075500"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFQ96_06500"
     CDS             complement(<1..450)
                     /locus_tag="VFQ96_06500"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0257472.1"
                     /translation="MRTDSVVSKPAQGTSFSKGLAVLGWILEHGAARADEIAPAVDLP
                     LSSVYRFLRTLREHGFVADADGLYDTGPRLASVGASPMNTSISTLAAPFLEHLSAVTG
                     ETAVLTVRQGLHAVCVSQVESAFQIRLAFELGQLLPLYAGAGQRVLLA"
     gene            526..2184
                     /locus_tag="VFQ96_06505"
     CDS             526..2184
                     /locus_tag="VFQ96_06505"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU0257473.1"
                     /translation="MADTVVVGAGRLTLEQLVAVARDGAHVELAPEAIARMRPSRDWV
                     EHAIADRGADGTIKPIYSINTGFGSLAGRAAFQDPAQAEDLSRRLIVSNACGVGPFLP
                     AEIVRAAMLIRISSLTCGFSGIRPVVVQTLVDMLNAGVTPAVPEIGSLGASGDLVPLA
                     HMMLVATRPAGDDDPEVDSGEAIVDGEIVSGIVAMHEAGIARVVLGAKEGLALTNGTS
                     FSTALAALAVYDAARLVTTAEITAAMSIEALTGFGDAFLPQIHDARGQRGQIEAAAHI
                     RALLAGSTLVDGNEQTDPVRQPPQDAYSLRCIPQVHGAVRDTLAFARGIVETEINAAT
                     DNPLIFPDLPDSRHLKAVSGGNFHAEYLAFVADFVAIAVTEIGSIAERRLFRLDDGTL
                     NRGLPDMLIDSTDVGMDCGYMLPQYVAAALVSDCKALAHPDSVDSIPTCANQEDHVSM
                     AANAGRHARSVIENITAVIGFELAMAAQALELRLAHPDYAGARLAPASRALVDLVRAT
                     PTETGGSITHLSRDVVMYPLLRAATGLVRSGAVLDTAAAHTDSA"
     gene            2294..>2869
                     /locus_tag="VFQ96_06510"
     CDS             2294..>2869
                     /locus_tag="VFQ96_06510"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257474.1"
                     /translation="MTASTGQHRTEFMSVSDAPFHPKPVVHDIKAQRGDVMRCKGPKQ
                     ETILRMLENNMEVSEYPDKLVVYGGIGKAARNWESYHAIVDSLKNLENDETLVVQSGM
                     PVAVFQTHELAPRVVMATTNFVRPSWEKFYELHDKNLTIFAQYTAAPWEYIGTQGVIQ
                     GTYETLRSVGNLHFGGDLAGRLLVCAGMGGMG"
BASE COUNT          479 a         1037 c          919 g          434 t
ORIGIN      
        1 ggcgagaaga acgcgctgcc cggcgccggc gtacagcggc aggagctgtc cgagctcgaa
       61 ggcgagccgg atctggaatg ccgactcgac ctggctcaca catacggcgt gcagcccctg
      121 ccggacggtg agcacggccg tctcgcccgt gacggccgac aggtgttcca ggaacggggc
      181 ggccagcgtc gagatgctcg tattcatcgg ggaggcgccg acgctggcca gccgggggcc
      241 ggtgtcatag aggccgtcgg catcggcgac gaaaccgtgt tcgcggagcg ttctcaagaa
      301 tcgatagacg ctggacagcg gcaggtcgac ggccggggcg atctcgtcgg cgcgggcggc
      361 gccgtgctcc agaatccagc cgagcaccgc cagacccttc gagaatgacg tgccctgcgc
      421 gggtttcgac accaccgaat cagttcgcat gatgtgagaa tagcagtttg caacacgtga
      481 ttacgcccct agcgtggtgg acattcggac acggtaggag gcctgatggc agacacagtg
      541 gtggtggggg ccgggcggct taccctcgag cagctcgtgg cggtcgcccg cgacggggcg
      601 cacgtcgagc tggcacccga ggcgatcgcc cgcatgcggc cgagccgcga ctgggtcgag
      661 cacgccattg ccgaccgggg cgccgacggc accattaagc cgatctactc catcaatact
      721 ggcttcggct cgctggccgg ccgcgccgcc ttccaggatc cggcccaggc cgaggacctc
      781 tcccgccgcc tcatcgtctc caacgcgtgc ggcgtcggcc cgttcctgcc agccgagatc
      841 gtccgcgccg ccatgctgat tcggatctcg agcctcacct gcggcttctc cggcatccga
      901 ccggtcgtcg tgcagacctt ggtcgacatg ctcaacgccg gtgtcactcc ggccgtcccc
      961 gagatcggaa gcctcggcgc ctccggcgat ctcgtcccgc tggcacacat gatgctcgtc
     1021 gccacccgcc cggccggcga cgacgatccc gaggtcgata gcggcgaggc catcgtcgac
     1081 ggcgagatcg tctccggcat cgtcgcgatg cacgaggccg gtatcgcgcg cgtcgtcctc
     1141 ggcgccaagg aagggctggc cctcaccaac ggcacctcgt tctccaccgc gctcgccgcc
     1201 ctggccgtgt atgacgccgc ccggctcgtc accacggccg agatcacggc ggccatgtcc
     1261 atcgaggcgc tgaccgggtt cggcgacgcg ttcctgccgc agatccacga cgcgcgcggc
     1321 cagcgtggcc agatcgaggc cgccgcacac atcagggcac tcctcgccgg cagcaccctg
     1381 gtcgatggca acgagcagac cgacccggtt cgtcagccgc cgcaggacgc ctactcgctg
     1441 cgctgcattc cgcaggtgca cggggcggtc cgggacacgc tcgccttcgc ccgcggcatc
     1501 gtcgagaccg agatcaacgc ggccaccgac aatccactga tcttccccga cctgccggat
     1561 tcgcggcacc tcaaggccgt ctccggcggc aatttccacg ccgaatacct cgccttcgtc
     1621 gccgacttcg tcgcgatcgc cgtcaccgag atcggctcca tcgccgagcg gcgcctgttc
     1681 cgcctggatg acggcaccct caaccgcggc ctgcccgaca tgctcatcga ctcgacggac
     1741 gtcggcatgg actgcggcta catgctgccg cagtatgtgg cggcggcgct cgtctcggac
     1801 tgcaaggcgc tcgcgcaccc cgactccgtc gactcgatcc ccacctgcgc caaccaggag
     1861 gatcacgtct cgatggcggc gaacgccggc cggcacgccc ggtccgtgat cgagaacatc
     1921 acggcggtca tcggtttcga gctcgccatg gccgcgcagg ccctggagct ccggctcgcg
     1981 caccccgatt acgccggcgc ccgcctcgcg ccggcgagcc gggcgctggt cgacctggta
     2041 cgcgccacgc cgaccgaaac cggcggcagc atcacgcatt tgagccgtga cgtggtcatg
     2101 tacccgctgc tgcgcgcggc gaccgggctc gtccgctccg gcgcggtgct cgacacggcc
     2161 gcggcccaca ccgactccgc ctgattcgac taggcccgac cccgacggaa acgtcatacc
     2221 cgtttttcac tcactcaccg tgcgacatca gctttaccgc aacgagtctc gtaatcgaaa
     2281 ggatgacatc agcatgaccg catcgacagg gcagcaccgg acagagttca tgtccgtctc
     2341 cgacgcgccc ttccacccga agcccgtcgt gcacgacatc aaggcccaac gcggcgacgt
     2401 gatgcgctgc aagggcccga agcaggagac gatcctgcgg atgctcgaga acaacatgga
     2461 ggtctccgag taccccgaca agctcgtcgt ctacggcggc attggcaagg cggcgcgcaa
     2521 ctgggagtcg taccacgcga tcgtcgactc gctgaagaac cttgagaatg acgagaccct
     2581 cgtggtgcag tccggcatgc cggtcgcggt cttccaaacc cacgagctgg caccgcgtgt
     2641 cgtcatggcg acgacgaact tcgtgcgccc gagctgggag aagttctacg agctgcacga
     2701 taagaacctc acgatcttcg cccagtacac cgcggccccc tgggagtaca tcggcaccca
     2761 gggcgtcatc cagggcacct acgaaacgct gcgcagcgtc gggaacctgc acttcggcgg
     2821 cgatctcgcc gggcgcctcc tggtgtgcgc gggcatgggc ggcatgggc
//
LOCUS       DASTNL010000206        11156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_707866, whole genome shotgun sequence.
ACCESSION   DASTNL010000206 DASTNL010000000
VERSION     DASTNL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707866"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFQ96_06515"
     CDS             complement(<1..189)
                     /locus_tag="VFQ96_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257475.1"
                     /translation="MNPAGTTTGRTVVVTGGNRGIGRRIAQRFVAAGDRVAVLCRSGE
                     GPEGTLTLRADVTDRTSVD"
     gene            360..674
                     /locus_tag="VFQ96_06520"
     CDS             360..674
                     /locus_tag="VFQ96_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HEU0257476.1"
                     /translation="MKKKQSITSLPLSPEDDRRSRMMHYSVAMGIRFVCIVALLFVHG
                     WWLILPGLGAVFIPYFAVVVANFAHNRGQEVERPGNVTLVTPPAPQGAGAADGARKAS
                     SV"
     gene            671..922
                     /locus_tag="VFQ96_06525"
     CDS             671..922
                     /locus_tag="VFQ96_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257477.1"
                     /translation="MSLVGEPPSRFTCSRAGCESPATWRIRWRNPKIHTPDRVKIWLA
                     CPEHIGYLRSFLGTRSFPMVVDDRLEDPPDPIPDATGDE"
     gene            933..1790
                     /locus_tag="VFQ96_06530"
     CDS             933..1790
                     /locus_tag="VFQ96_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEU0257478.1"
                     /translation="MHRWHFVFSRRWFVYIACAIVFAIACGFLSHWQLTQSRDAQAAN
                     RLVARNFDSSVTALTDLLPRPGTFRADMEWRRVRATGSYEPQNQLIVRNRPTDAGPGF
                     EVLTPFRLADGAVFIVDRGWVPVGTGDDPAPVAAPPEGTVSVTMRLKPGEPALKGRSV
                     IGDQIPSIDLPWIRRHLGETTYTAAYGLLQSQTPKPAHSLTAVSATPPTQNTGMYWSY
                     MIQWLIFAAIAFFGVGYAARSEYRRRYQDEPDMVLKEQERQERAGRRKLTDAEIEDAL
                     LEESWPPAH"
     gene            complement(1795..3390)
                     /locus_tag="VFQ96_06535"
     CDS             complement(1795..3390)
                     /locus_tag="VFQ96_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0257479.1"
                     /translation="MLTVQDLTIRVGARTLMSGVSFHVGTGDKVGLVGRNGAGKTTLT
                     KVLAGEVLPTAGHVTTGEEIGYLPQDPRSGDPEETARARILDARGLGSLVAGMQRATE
                     RMAVPGEAADEAMRAYSRLEERFQVLGGYAAEAEAAAICSNLGLPDRVLQQQLGTLSG
                     GQRRRVELARILFSDATTMILDEPTNHLDADSVGWLREFLRGYPGALIVISHSIELVD
                     ETVNRVFYLDANRAVIDVYNMGWQQYQRQRAADEARRRKERANAEKKASALQQQAARF
                     GAKATKAAAAHQMEARARRLLDGLEEVRADDRVANIRFPEPAPCGRTPLQAHNLSKSY
                     GSLEIFSAVDLAIDRGSRVVVLGLNGAGKTTLLRILAGIDRPDTGAVEAGHGLRIGYY
                     AQEHETIDVSQTILENMVSVSPDLDQTQARRILGSFLFTGDDVYKSSKVLSGGEKTRL
                     ALAMLVVSSANVLLLDEPTNNLDPASREKILDALAHYPGAVVLVSHDEGAVESLNPER
                     VLLLPDGVEDHWNHDYTELVSLA"
     gene            complement(3540..3836)
                     /locus_tag="VFQ96_06540"
     CDS             complement(3540..3836)
                     /locus_tag="VFQ96_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU0257480.1"
                     /translation="MIEEALKYVIDPELGVNVVDLGLIYDLAWDDAKDELYITMTLTS
                     AGCPLSDAIEEQIVDSLDGVVPGFRINWVWMPPWGPEMISDDGREMMRSIGFTV"
     gene            complement(3927..4694)
                     /gene="sufC"
                     /locus_tag="VFQ96_06545"
     CDS             complement(3927..4694)
                     /gene="sufC"
                     /locus_tag="VFQ96_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU0257481.1"
                     /translation="MTSVLEIRDLHVSVDTEHGAKEILRGATLTLRQGEVHAIMGPNG
                     GGKSTLAYTIAGHPKYHVTSGSITLDGADVLSMLVDERARAGLFLAMQYPVEIPGVTN
                     TNFLRTAKTAIDGKAPALRDWTTSMRETMKKLRMDKTFAQRNVNEGFSGGEKKRNEIL
                     QLELLKPKFAVLDETDSGLDVDALKIVSEGVNRAHEATGLGILLITHYTRILRYITPD
                     FVHVFVAGRVAEEGGPELADRLEEEGYDRYTSAASLA"
     gene            complement(4723..5928)
                     /locus_tag="VFQ96_06550"
     CDS             complement(4723..5928)
                     /locus_tag="VFQ96_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEU0257482.1"
                     /translation="MSDTATKTVQKGTESDKHTLQANGGWGDLKIPPQSRAERVSSTA
                     VDDFPPVTGREHEWMHTPVSRLTALTAGTLAGGEYDVSYEPTEGVSVSWVGRDDPRVG
                     SAGKPEDRASANAWSAFEKALVITIDGEVSGEFTLTRQGFGEAPHAAHVIIEAKPFGK
                     AVLVVRSVGPAQLSENVEIIAGESSQLTVVSDQEWDPDALHLASQFSRVEKDAHYKHV
                     LASLGGSVVRVNPSAHLTGAGSDVEMLGVYFADAPQHLEQQVFVYHDAPHCRSRVTYK
                     GALNGAGARTVWVGDVLIGKRGIGTDSYEQNRNLVLSQGARADSEPNLEIEVGDIIGA
                     GHASATGRFDDEQLFYLQSRGIAKDEAMRLVVRGFLVDVVQKIGAPTLEAHLIEHIED
                     ELTKAYATA"
     gene            complement(5929..7347)
                     /gene="sufB"
                     /locus_tag="VFQ96_06555"
     CDS             complement(5929..7347)
                     /gene="sufB"
                     /locus_tag="VFQ96_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU0257483.1"
                     /translation="MSDTLIDRPELAGLGNYEFGWADSDKAGANAQRGLSEDVVRNIS
                     ALKNEPEWMMKRRLKALKIFQRKPMPNWGADLSGLDLEHIKYFVRATERQAQRWEDLP
                     EEVRSTYEKLGIPEAERENLIGGVTAQYESEVVYRQIRDDLSSQGVIFTDTDTGLREH
                     PEFFEEYFGTIIPPGDNKFAALNTAVWSGGSFVYVPPGVHVQIPLQAYFRINTENMGQ
                     FERTLIIADEGSYVHYIEGCTAPEYSSDSLHSAVVEIVAKKNSRVRYTTIQNWSGNVY
                     NLVTKRTIAHEGATMEWVDGNIGSKVTMKYPSVWLVGEHAKGETLSVAFAGPGQHQDA
                     GSKMVHMAPNTHSSIVSKSLARGGGRSGYRGEVRMEPSAHHSSNTVRCDALLVDKQSR
                     SDTYPTIDIRVDDVELGHEATVSRVSEEQLFYLMSRGLAEAEAMAMIVRGFIEPVARE
                     LPMEYALELNKLIEMGMEGSVG"
     gene            complement(7596..8540)
                     /locus_tag="VFQ96_06560"
     CDS             complement(7596..8540)
                     /locus_tag="VFQ96_06560"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU0257484.1"
                     /translation="MDVAASAQGTGTRTAPYTSFGNFLGRKFTAYLALTKPRIIELLL
                     VVTVPVMFLAQRGIPNLWLVVATVAGGFLSAGSAGAFNCYIDRDIDRVMHRTRNRPLV
                     TGELSDREAFIFCWVLAVASTVILGVFANWLTAALSVIGILLYAGFYTLVLKRRTQQN
                     IIWGGIAGCMPVFIGWAAVTNTIAWPAVVLFVVIFLWTPPHYWPFSMRFRDDYREVGV
                     PMLAVVRGRAVVGIQVILYTWATVACTLLLIPVAHMGVVYTVVAAAAGAWFIYEAHHL
                     YNTAIRHRTVVPMRVFHASITYLTLVFLAIAIDPLLTL"
     gene            8786..10885
                     /gene="tkt"
                     /locus_tag="VFQ96_06565"
     CDS             8786..10885
                     /gene="tkt"
                     /locus_tag="VFQ96_06565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0257485.1"
                     /translation="MTAIPWDAIDKKAVDTVRLLAADAVEKAGSGHPGTAMSLAPVGY
                     LLFQRILRHDPAEPDWAGRDRFILSVGHSSLTQYIQLYLGGYGLELEDLKQLRKWGSK
                     TPGHPEYRHTRGIEITTGPLGQGISSSVGFAYAARYERALLDPDTPAGKSVFDHHIFV
                     IAGDGDLQEGISSEASSLAGHQQLGNLVVLYDSNQISIEDDTNIAFTEDVRARYEAYG
                     WHVQVVDWKKTGAYVEDVQELNDAIEAAKAVTDKPSLIVLKTIIGWPSPTKQNSGAIH
                     GAKLGADELAALKKVLGADPAASFQVAPEVLAHTRQARERGAARHRAWDERFAAWAAA
                     HPDKKALFDRMRKGELPEGLEAALPVFDTAKAISTRSASGKVINALAPVLPELWGGSA
                     DLAGSNNTTIEGAASFAPAEHSTKEWKADESGRVLHFGIREHAMGAIINGIVLHGNTR
                     PFGGTFLIFSDYMRPAVRLAALMDIPSIFVWTHDSIALGGDGPTHQPVEQLTALRAIP
                     NLEIVRPADANETAYAWYETLRRHRHPTGFALSRQNLPVFERGTGEAHGEVLAAASLT
                     ARGAYVLAEAPNGTPDVILIATGSEVQYALAARTTLAAGGVNARVVSAPCLEWFADES
                     AEYRASVLPPTVKARVSVEAGISLGWRPYIGDAGRSVSVERFGSSADPDVLYREYGIT
                     ADAVVHAAQESLAAARG"
     gene            10942..>11156
                     /locus_tag="VFQ96_06570"
     CDS             10942..>11156
                     /locus_tag="VFQ96_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012903580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEU0257486.1"
                     /translation="MTSRTQQLTDAGVSIWLDDLSRERMTSGGLKHLIDERNVVGVTT
                     NPTIFAKALTGTEAYDAQIRELSAAGA"
BASE COUNT         1806 a         3750 c         3673 g         1927 t
ORIGIN      
        1 gtcgacgctc gtcctgtcgg tgacgtcggc gcgcaacgtc aaggtgcctt ctggcccctc
       61 gccgcttcgg cagagtacgg cgacccggtc gccggccgcg acgaaacgtt gggcgatcct
      121 cctgcctatc cctcggttgc cgcccgtgac gacgacggtg cgcccggtgg tggtgcctgc
      181 agggttcatc tcggcctttc cttcggtgtg tcccgcctcg gatatcggtc ggggcccacg
      241 gttcgtcggc aggtttcgct agtctgtcag cagacaccaa gtgtacgagg gcggccaagt
      301 gtctttcgtc gtcgtagtct tgagtgaggc tgcgattcga tcgcggcgtt gggcaggaca
      361 tgaagaagaa gcagtcgatc accagcctgc cgctctcgcc cgaagacgac cggcggtcac
      421 ggatgatgca ttactccgtg gcgatgggca ttcgttttgt ctgcatcgtc gcactgctgt
      481 tcgtgcatgg ctggtggctg atcctgccgg ggttgggtgc cgtgttcatc ccctacttcg
      541 cggtcgtcgt cgcgaacttc gcccataacc ggggccaaga ggtcgagcgg ccgggcaatg
      601 tgaccctcgt cactccgcct gccccgcagg gcgcgggcgc cgccgacggc gcgcggaagg
      661 cctcgtcggt atgagcctcg tcggcgagcc accgtcgcgc ttcacctgtt cgcgggccgg
      721 atgcgagagc ccggccacct ggcgcatccg gtggcgcaac cccaagatcc acacccccga
      781 cagggtgaag atctggctgg cgtgccccga gcacatcggc tacctgcgct cgtttctcgg
      841 cacgcgcagc tttcccatgg tcgtcgatga caggctggaa gacccgccgg atcccattcc
      901 cgacgccacc ggcgacgagt gaggacgttg tcatgcaccg ctggcacttc gtcttctctc
      961 ggcgctggtt cgtctacatc gcctgcgcga tcgtgtttgc catcgcgtgt gggttcctct
     1021 cccactggca gctgacgcag agccgcgacg cgcaggccgc aaaccggctg gtcgcccgca
     1081 acttcgactc gtcggtgacg gccctgaccg acctactgcc gcggcccggc acatttcggg
     1141 ccgacatgga atggcgtcgg gtgcgtgcca ccggaagcta cgaaccccag aatcagctga
     1201 tcgtgcgcaa ccgaccgacg gacgccgggc ccgggttcga ggttctcacc ccgttccggc
     1261 tcgccgacgg cgccgtgttc atcgtcgacc gaggctgggt gccggtcggc accggggacg
     1321 accccgcacc ggtcgcggcc ccgccggaag gcacggtgag cgtcacgatg cgcctgaagc
     1381 cgggcgagcc ggctttgaaa gggcgctcgg tgatcggcga ccagatcccc agcatcgacc
     1441 tgccgtggat tcggcggcac ctgggcgaga cgacctacac cgccgcgtac ggcctgctgc
     1501 agtcgcagac gccgaaaccg gcacactcgc tgacggcggt ctctgccacc ccgccgaccc
     1561 agaacaccgg catgtactgg tcgtacatga tccagtggct catcttcgcg gcgatcgcgt
     1621 tcttcggcgt cggctatgcg gcgcgctcgg aataccggcg ccggtaccaa gacgagcccg
     1681 acatggtgtt gaaagagcaa gagaggcagg agcgggccgg ccggcgcaag ctcaccgacg
     1741 ccgagatcga ggatgcgctc ctcgaggagt cgtggccgcc cgcacactga ggcctcaggc
     1801 cagcgacacc agctcggtgt agtcgtggtt ccaatggtct tcgacgccgt cgggcaaaag
     1861 gagcacgcgt tccgggttca gtgattcgac ggcgccctcg tcgtggctga cgaggacgac
     1921 ggcgcccggg tagtgggcga gcgcgtcaag gatcttctcg cggctggccg gatcgaggtt
     1981 gttggtcggc tcgtcgagga gcagcacgtt ggcgctggag acgacgagca tcgccaacgc
     2041 cagacgggtc ttctcgcccc cggagagcac cttgctgctc ttgtacacgt cgtcgccggt
     2101 gaagaggaac gacccgagga tgcggcgggc ctgcgtctgg tcgaggtcgg gcgagaccga
     2161 gaccatgttt tcgaggatcg tctgcgagac gtcgatcgtc tcgtgctctt gggcgtagta
     2221 cccgatgcgc aacccgtgcc cggcctccac cgccccggta tccggcctgt cgatgccggc
     2281 gaggatgcgc aggagcgtcg tcttgcccgc gccgttcagg ccgagcacga cgacgcggga
     2341 cccgcggtcg atcgctaggt cgacggcgct gaagatctcc aaagacccgt agctcttcga
     2401 caggttgtgt gcctgcaacg gggtgcggcc gcacggtgcc ggctccggga aacggatgtt
     2461 cgccacccga tcgtcggccc gcacctcctc gaggccgtcg aggaggcgcc tggcacgggc
     2521 ctccatctgg tgtgcggccg cggccttcgt tgccttcgcg ccgaaccgcg cggcctgctg
     2581 ctgcagggcg ctcgccttct tctcggcgtt ggcccgttcc ttgcgccggc gggcctcgtc
     2641 ggcggcccgt tggcgctggt actgctgcca gcccatgttg tagacgtcaa tcacggcgcg
     2701 gttcgcgtcg aggtagaaga cgcggttgac ggtctcgtcg acgagctcga tgctgtggct
     2761 gatcacgatg agcgcgcccg ggtagccgcg caagaattct cgcagccaac cgaccgaatc
     2821 ggcgtcgagg tggttcgtcg gctcgtcgag aatcatcgtc gtggcgtcgg agaagaggat
     2881 gcgggcgagc tcgacgcggc gccgctggcc gcccgagagc gtgccgagct gctgctggag
     2941 gacccggtcg ggcagcccaa ggttgctgca gatcgcggcg gcctccgcct cggccgcgta
     3001 gccgcccaga acctggaagc gttcttccag ccgggagtag gcgcgcatcg cctcgtcggc
     3061 ggcctcgccc ggcaccgcca tccgttccgt cgcgcgctgc atgcccgcca cgaggctgcc
     3121 gaggccccgg gcgtccagga tcctggcacg agccgtctcc tccgggtcgc cgctgcgcgg
     3181 gtcctgcggc aggtagccga tctcctcacc cgtcgtgaca tgccctgccg tcgggagcac
     3241 ctcgcccgcc agcactttcg tcagagtcgt cttgcccgcc ccgttgcggc cgacgaggcc
     3301 caccttgtcg ccggtgccca cgtggaaaga gacgccggac atcaaggtgc gtgcgccgac
     3361 gcggatggtc aggtcttgca cggtgagcac ggaatcatcc aatcgtgtcg tccgggcggc
     3421 cgtgtggccg ggcctcaggg tacggagcct gcaggccgct tctgcccggc cggcgtcagc
     3481 cggcaaaacg cacccgcaca gatcactgtg cgggtgcgtc ttcttcgtcg agggcctgtc
     3541 tagacggtga agcctatgct ccgcatcatc tcacgcccgt cgtcgctgat catttcggga
     3601 ccccacggcg gcatccacac ccagttgatg cggaacccgg gaacgacccc gtcgagggag
     3661 tcgacgatct gctcctcgat cgcgtcggag agcgggcagc ccgcactcgt cagcgtcatc
     3721 gtgatgtaga gctcgtcttt cgcgtcgtcc cacgccaggt cgtagatgag gcccaggtcg
     3781 acgacgttca cgccaagctc ggggtcgatg acgtatttca acgcctcttc gatcatgtcg
     3841 tagtctgacg cgctgagcgg cgagatttgc gcctcccctt gcggggccgc ggtctgcgtc
     3901 tcggtcatgg cttcactcta cggttctcac gcgagactcg ccgcggaggt gtagcggtcg
     3961 tagccttctt cttcgagccg gtcggccaac tccgggccgc cttcctccgc gacccgccct
     4021 gccacgaaga cgtgcacgaa atcgggcgtg atgtaacgca gaatccgcgt gtagtgcgtg
     4081 atgaggagga tcccgaggcc ggtcgcctcg tgtgctcggt tgacgccttc cgagacgatc
     4141 ttcaacgcgt cgacgtcgag gccggagtcg gtctcgtcga gcaccgcgaa cttcggcttc
     4201 agaagctcca gttgcaagat ctcgttgcgc ttcttctcgc cgccggagaa accctcgttg
     4261 acgttgcgct gggcgaacgt cttgtccatc cgcagcttct tcatcgtctc gcgcatgctg
     4321 gtcgtccagt cgcgcagcgc gggcgccttg ccgtcgatcg ccgtcttcgc cgtgcgcaga
     4381 aagttcgtgt tcgtgacacc cggtatctcg accgggtatt gcatcgcgag gaacagcccg
     4441 gcacgggctc gctcgtcgac gagcatggac agcacgtcgg cgccgtcgag ggtgatcgag
     4501 ccgctcgtta catggtattt cgggtggccc gcgatcgtgt acgcgagcgt cgacttgccg
     4561 ccgccgttcg ggcccatgat cgcatgcacc tccccctggc gaagggtcaa ggtcgcgccg
     4621 cgcaagatct ccttggcgcc gtgctccgtg tcgacggaga cgtgcaggtc gcgtatttcg
     4681 agtactgagg tcatgaggtg tgaaggtgtc tttcggttac ggtcacgccg tcgcgtaggc
     4741 cttggtgagt tcgtcttcga tgtgctcgat gaggtgcgcc tcgagcgtgg gcgccccgat
     4801 cttctgcacc acgtcgacga ggaagccgcg cacgacgagg cgcatcgcct cgtctttcgc
     4861 gatgccgcgg gactgcaagt agaagagctg ctcgtcgtcg aaccggccgg tcgcgctcgc
     4921 gtggccggcg ccgatgatgt cgcccacctc gatctccagg ttcggctccg aatcggcgcg
     4981 cgcgccctgc gagagtacaa ggttgcggtt ctgctcgtag ctgtcggtgc cgatgccccg
     5041 cttgccgatg agcacgtcgc ctacccagac ggtgcgtgcc ccggccccgt tgagggcacc
     5101 cttgtaggtg accctgctgc ggcagtgcgg cgcgtcgtga tagacgaaca cctgctgttc
     5161 caagtgctgc ggcgcatcgg cgaagtagac gccgagcatc tccacatcgc tgcccgcacc
     5221 ggtgaggtgg gcggaggggt tcacgcgtac gacgctgccg ccgagagagg ccagcacgtg
     5281 cttgtaatgc gcgtctttct cgacccgcga gaactggctg gcgaggtgca acgcgtcggg
     5341 atcccattcc tggtcgctca ccaccgtgag ctgggaggac tcgccggcga tgatctcgac
     5401 gttctcgctc agctgggccg ggccgacgct gcgcacgacg aggacggcct tgccgaatgg
     5461 cttcgcctcg atgatcacgt gagcggcgtg cggcgcctcg ccaaaccctt gtcgggtgag
     5521 cgtgaattcg cccgacactt cgccgtcgat cgtgatgacg agcgccttct cgaaggcaga
     5581 ccacgcgttc gccgaggcgc ggtcttccgg cttgccggca gatcccacgc gcgggtcgtc
     5641 gcggcccacc cagctgacgc tcacgccttc ggtcggctca tacgagacgt cgtattcgcc
     5701 acccgcgagg gtgcccgcgg tgagcgccgt cagcctgctg accggcgtgt gcatccattc
     5761 gtgctcacgg ccggtgacgg ggggaaagtc gtcgaccgcc gtcgaggaga cccgttccgc
     5821 cctgctctgg ggcggaatct tcaggtctcc ccaacctccg ttcgcttgaa gggtgtgctt
     5881 gtcggattcc gtgcctttct ggacagtctt ggtggctgtg tctgacattt aaccgacgga
     5941 tccttccatg cccatctcga tgagcttgtt gagctcgagc gcgtattcca tgggcagctc
     6001 cctggcgacg ggctcgatga agccgcgcac gatcatggcc atggcctctg cctcggcaag
     6061 cccgcggctc atgaggtaga acagctgctc ctcgctgacc ctggagacgg tcgcctcgtg
     6121 gccgagctca acgtcgtcga cgcggatgtc gatcgtcggg taggtgtcgc tgcgcgactg
     6181 cttgtcgacc aggagggcgt cgcaacgcac cgtgttcgag gagtggtggg cggaaggctc
     6241 catgcgcacc tcgccgcggt agccgctgcg gccgccgccg cgggcgagcg acttcgagac
     6301 gatcgacgaa tgcgtgttag gcgccatgtg gaccatcttg gaacccgcat cctggtgctg
     6361 ccccgggccc gcgaacgcga ccgacagggt ctcacccttc gcgtgctcgc cgaccagcca
     6421 tacagacggg tacttcatcg tcaccttcga gccgatgttc ccgtcgaccc attccatggt
     6481 ggcgccctca tgggcgatcg tgcgcttcgt caccaggttg tagacgttgc ccgaccagtt
     6541 ttgaatggtc gtgtaacgca cccgggaatt cttcttcgca acgatctcga cgaccgcgga
     6601 atgcagggag tcggaactgt actccggcgc cgtgcagcct tcgatgtagt gcacgtaaga
     6661 gccctcgtcg gcgatgatca aggtgcgctc gaactgcccc atgttctccg tgttgatgcg
     6721 gaaatacgcc tgcaacggga tctgcacgtg cacgcccggc ggcacgtaga cgaacgaccc
     6781 gcctgaccac acggccgtgt tcagggcggc aaacttgttg tcccccggtg ggatgatcgt
     6841 gccgaagtac tcttcgaaga actcggggtg ctcacgcaga cccgtatcgg tgtcggtgaa
     6901 gatgacgccc tgcgaggaga ggtcgtcgcg gatctgccgg tagaccacct cggactcgta
     6961 ctgggcggtg acgccgccga tcaggttctc gcgctccgcc tcggggatgc caagcttctc
     7021 gtaagtgctg cgcacctctt caggcaggtc ttcccaacgt tgtgcctggc gttccgtcgc
     7081 acggacgaaa tacttgatgt gctccaggtc gaggcccgag agatccgcac cccagttggg
     7141 catgggcttg cgttggaaga tcttcagcgc cttcaagcgc ctcttcatca tccattccgg
     7201 ctcgttcttc aacgcggaga tgtttctgac gacgtcttcc gaaagacccc gctgcgcgtt
     7261 cgcgcctgcc ttgtcggagt cggcccagcc gaactcgtag ttgccgagtc cggccagctc
     7321 ggggcgatcg atcagagtgt ctgacatttc tacctttcct gccacggcga ccgaagacga
     7381 ctccggctca tcccggcgcc gaaagacgct cgggccgaga tgatggcgca tggctggcct
     7441 gtgtacctag atgctcaggg actagggcga cgctgccccg gcaggcaccg cgacgcgacg
     7501 cccacgcgtg ccttgcggca cgaccctcaa cctcactcga ttctacaggt cgagacccga
     7561 accgccaggg gcttcctcgc gcgggcgtgc ggccgtcaca acgtgaggag cgggtcgatc
     7621 gcgatcgcca ggaacaccag ggtcagatag gtgatcgagg cgtggaacac gcgcatcggc
     7681 accacggtgc gatgccggat ggccgtgttg tagaggtggt gcgcctcgta gatgaaccag
     7741 gcgcctgcgg ccgcggcgac gaccgtgtag accacgccca tgtgcgcgac cgggatgagc
     7801 agcagcgtgc aggccaccgt cgcccaggtg tacaggatga cctggatgcc gaccaccgca
     7861 cggccgcgca cgactgcgag catcggcacc ccgacctcgc gatagtcgtc gcggaagcgc
     7921 atggagaagg gccaatagtg cggcggggtc cataggaaga tcacgacgaa gagcaccacc
     7981 gccggccacg cgatcgtgtt cgtgaccgcc gcccagccga tgaacaccgg catacatccg
     8041 gcgatgccgc cccagatgat gttctgctgc gtgcgcctct tcagaacgag cgtgtaaaag
     8101 ccggcgtaga gcaggatgcc gatgacggag agcgcggccg tcagccagtt cgcgaagacg
     8161 ccgaggatca cggtggaggc gaccgcgagc acccagcaga agatgaacgc ctctcggtcg
     8221 ctcaactccc ccgtgacgag tggacggttg cgggtgcggt gcatcacacg gtcgatgtcg
     8281 cgatcgatgt aacagttgaa ggcccccgcc gacccggcgc ttaaaaaccc gcccgcaacc
     8341 gtcgcgacga cgagccagag gttcgggatg ccgcgctggg cgaggaacat cacgggcacg
     8401 gtgacgacga gcagcagctc gatgatgcga ggcttcgtca gtgcgaggta ggccgtgaac
     8461 ttacggccga gaaagttgcc gaaagaggtg tatggagcgg tgcgtgtgcc cgttccctgt
     8521 gccgatgcgg cgacgtccat tgcacctcgt gatcccactg aacaactcca caacgattct
     8581 aagccacgtg ccaaggcggg gcccggcgcc cacacgccaa ccactatgct ggatgacagc
     8641 tcgccaatcg agcgatagac aggagaatcg gacgctccga caaccctgtc acgccgatgt
     8701 cgaagacgct agcggcgcgt actcagcacg ggtgcgagtg agacctcgac ccgtttcttt
     8761 gtcagatgcg aaaggtcagg ttcccgtgac tgcaatccct tgggacgcga tagacaagaa
     8821 ggctgtcgac accgtgcggc tcttggccgc agacgccgtg gaaaaagcag gaagcgggca
     8881 tccgggcacg gcgatgagcc tcgcccccgt cggctacctg ctctttcagc gcatcttgcg
     8941 ccatgacccc gccgaacccg actgggcggg acgcgaccgc ttcatcctct cggtggggca
     9001 cagttccctc acgcagtaca ttcagctgta cctcggcggc tacggcctgg agctcgagga
     9061 cctgaagcag ctgcgcaaat ggggttcgaa gacccccggc cacccggagt accggcacac
     9121 ccgcggcatc gagatcacca cggggcccct cggccaaggc atctcgtcct ccgtcggctt
     9181 cgcctatgcg gcccgctacg agcgggcgct tctcgacccc gacacccccg ccggcaagag
     9241 cgtcttcgat caccacatct ttgtcatcgc gggcgacggc gaccttcaag aaggcatctc
     9301 cagcgaggca tcgtctctcg cgggccatca gcagctcggc aacctcgtcg tgctctacga
     9361 cagcaaccag atctcgatcg aagacgacac gaacatcgcc ttcaccgaag acgtgcgggc
     9421 ccgctatgag gcgtacggct ggcacgtcca ggtggtggat tggaagaaga ccggcgccta
     9481 cgtcgaagac gttcaagagt tgaacgacgc gatcgaggcg gccaaggccg tgaccgacaa
     9541 gccgtctctc atcgtcctga agaccattat cggctggccg agcccgacga agcagaacag
     9601 cggcgccatc cacggcgcca agctcggcgc cgacgagttg gcggcgttga agaaggtgct
     9661 gggagccgac ccggccgcat ccttccaggt ggcccccgag gtcttggccc acacgcgtca
     9721 ggcgcgcgag cgcggcgcgg cccgccatcg cgcctgggac gagaggttcg cggcctgggc
     9781 ggcggcgcac cccgacaaga aggccctctt cgacaggatg cgcaagggcg agctgcccga
     9841 gggcctcgag gcggcgctgc ccgtcttcga cacggcgaag gcgatctcga cgcgttccgc
     9901 cagcggcaag gtcatcaacg cgttggcgcc ggtgctcccc gagctgtggg gcggctccgc
     9961 cgacctcgcg gggtcgaaca acacgacgat cgagggcgcc gcctcgttcg ccccggccga
    10021 gcactcgacg aaggagtgga aggccgacga aagcgggcgg gtgctgcact tcggcatccg
    10081 cgagcacgcc atgggcgcga tcatcaacgg gatcgtgttg cacggcaaca cgcggccgtt
    10141 cggcggcacg ttcttgatct tcagcgacta catgcgcccg gcggtgcgcc tcgcggctct
    10201 catggacatc ccttcgatct tcgtctggac gcacgattcg atcgcgctcg gcggtgacgg
    10261 cccgacccac cagcccgtcg agcaattgac ggcgctgcgc gccattccga acctcgagat
    10321 cgtgcggccg gccgatgcga acgagaccgc gtacgcgtgg tacgagacgc tgcgccggca
    10381 ccgccacccg accggattcg cgctcagcag gcagaacctg ccggtcttcg agcgcggcac
    10441 cggagaggcg cacggcgagg tcctggccgc cgcctccctg acggcccggg gcgcctacgt
    10501 gctcgccgag gcgccgaacg gcacccccga cgtgatcctc atcgccaccg gctccgaggt
    10561 gcagtacgcc ctggccgccc gcacgacgct cgccgccggc ggcgtgaacg cgcgcgtcgt
    10621 ctcggcaccg tgcctcgagt ggttcgccga cgagagcgcc gaataccgcg cgtcggtgct
    10681 gccgcccacc gtcaaggcgc gggtctccgt cgaggcgggc atctcgcttg gctggcgccc
    10741 ctacatcggc gacgcgggcc gcagcgtctc ggtcgaacgc ttcggctctt ccgccgaccc
    10801 cgacgtgctg taccgcgaat acggcatcac cgccgacgcg gtcgtccacg cggcccagga
    10861 atctctcgct gccgcccgcg gttgagactg ccgagaccac gcggcacggg cagacaagct
    10921 cagaacagga tggagcaggc aatgacaagt cgcacccaac agctcaccga tgcgggcgtg
    10981 agcatctggc tcgacgatct ctcgcgcgaa cggatgacct ccggcgggtt gaagcatctc
    11041 atcgacgagc gcaacgtcgt cggggtcacc acgaacccga ccatcttcgc caaggccctg
    11101 acgggcacgg aggcttacga cgcgcagatc cgcgagctct ccgccgccgg cgcaga
//
LOCUS       DASTNL010000207         8135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_895181, whole genome shotgun sequence.
ACCESSION   DASTNL010000207 DASTNL010000000
VERSION     DASTNL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895181"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFQ96_06575"
     CDS             complement(<1..822)
                     /locus_tag="VFQ96_06575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEU0257487.1"
                     /translation="MNVSEPVASGPNAAGRPPGASGDLPAAGRDAAGAWPTWAAALAG
                     ATGGLRAAGIASARTDAVLLAGHLLGASRGRVEAMAIAGAALSEREAARYAAMVERRA
                     GHEPLQHIVGTAAFRTLVLDVGPGVFVPRPETEMVAQLAIDLLRAAGPGALGVDLGTG
                     TGAIALSLAVEVPDARVVAVEASGAACSFARRNIARIAPGVRLVYGDLRAALPGVDGA
                     ADVVVSNPPYVPARERPPEPEARRDPPRALYGGPDGLAVIRVLARRAAVLVRPGGA"
     gene            916..1848
                     /gene="cysK"
                     /locus_tag="VFQ96_06580"
     CDS             916..1848
                     /gene="cysK"
                     /locus_tag="VFQ96_06580"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU0257488.1"
                     /translation="MTRIHADITTAVGGTPLVQLNHLTNDASARVLAKLEFYNPAGSV
                     KDRIGVAMVDAAEAQGTLRPGGTIIEATSGNTGIALAMVGAARGYRTIIVMPEKTSKE
                     RRILIRAYGAEVILTGPGGMVEANRKVREILEADPEAVWLNQFENAVNPGIHHDTTGQ
                     EIWDDTDGKVDVFVAGVGTGGTLTGAARLLKEKKPAVHVVAVEPAESPLLTGGEPGPH
                     RISGIGANFIPPVLDRDIYDEVADVPSSEALDMARALASQEGIFSGVSGGAACAAALV
                     QAAKPENAGKTIVFVVPDFGERYLSTSLYRHLAD"
     gene            1881..2471
                     /gene="epsC"
                     /locus_tag="VFQ96_06585"
     CDS             1881..2471
                     /gene="epsC"
                     /locus_tag="VFQ96_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEU0257489.1"
                     /translation="MSVLSRIREDIETAKRRDPAARSGTEILLGYSGLHAIWAYRLAH
                     RWWKAPGMKLPARLLSQLTRSVTGVEIHPGARIGRRFFIDHGMGIVIGETAIVGDDVM
                     LYHDVTLGGRGSRPGKRHPTLGDGVVVGAGAQLLGNITIGPRCQVGANAVVVRDAPAD
                     SVLVGIPARVLPRRVAGRDRQDHDPNEWIDPTFYVI"
     gene            complement(2539..3627)
                     /gene="prfA"
                     /locus_tag="VFQ96_06590"
     CDS             complement(2539..3627)
                     /gene="prfA"
                     /locus_tag="VFQ96_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU0257490.1"
                     /translation="MFESIDTLLDEHARLEGQLADPTLHANAARARKVGRRYAELSRV
                     KAAYDAWHALQGDLQAARELADEDATFAEEIPGLAERLALAEEELRRLLVPRDPDDGR
                     DVIMEIKGGEGGEESALFAGDLLRMYLHYAASKGWTAELLGQTESDLGGYKDVQLAIK
                     SSNNDPDGGVWAHLKYEGGVHRVQRVPVTESQGRIHTSTTGVLVFPEVDEPDEIEISQ
                     ADLKIDVYRSSGPGGQSVNTTDSAVRITHLPTGIVVSMQNEKSQLQNKEAGMRVLRAR
                     LLARQQEEQAAAGAAARHSQIRAMDRSERIRTYNFPENRIADHRTGFKAYNLDQVMDG
                     ALDAVIASCVRADEEARLAELGTGAAES"
     gene            complement(3635..5710)
                     /gene="rho"
                     /locus_tag="VFQ96_06595"
     CDS             complement(3635..5710)
                     /gene="rho"
                     /locus_tag="VFQ96_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU0257491.1"
                     /translation="MTNVNDSSATPADEALPGLRVAELQAVAARLGITGSARLRKSEL
                     VERIRAVRGGAAPAPAGRRPRRATSADIQLPMPEAGPAAEAPAEPAAEVPPAAEVPPA
                     AEAAPPVASKARQGGRRARGGGSAAGQAPVVAAADHAGGGRAGVDLGASGVSEGGAAA
                     EPARTDRARGRGRLEVDAAGRPAAASDAGEGAEGREGGADEHVTGGEQAGRERTTRGR
                     SRNRKNGEHRHTEAQQGAGRDTAERSGEGAEAGEAGEAEHSGRQQRGRQGQQSQKENG
                     NGEHRERGRGRGRDRKHRGSKEEAEPEILEDDVLVPIAGILDVLDNYAFVRTTGYLPG
                     ASDVYVSLGQVRKYALRKGDAVVGAVRQPREGEGSGRQKYNALVKVDSINGQSVEEAQ
                     HRVQFADLTPLYPQERLRMETEPGKVTQRVIDLVAPIGKGQRGLIVAPPKAGKTIVMQ
                     QIANAIAKNNPEVHLMVVVVDERPEEVTDMQRTVNGEVIASTFDRPAEDHTTVAELAI
                     ERAKRLVELGHDVVVLLDSITRLGRAYNITAPTSGRVLSGGVDAAALYPPKRFFGAAR
                     NIEHGGSLTILASALVETGSRMDEVIFEEFKGTGNSELRLSRQLADKRIFPAVDVSAS
                     GTRREELLMGADEVNVTWKLRRALAGLDQQQALEAVLGRIKETSTNVEFLMQMQKSLP
                     GAAGHVDAR"
     gene            6319..6597
                     /locus_tag="VFQ96_06600"
     CDS             6319..6597
                     /locus_tag="VFQ96_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257492.1"
                     /translation="MSKVNALPLVAVSNEVPKMMKHRVPSQFRKNDPPRTSVARNDSP
                     WKTAASGVATSSIVIPADLATACAASRSPERSSFVASRRAMNCGVSLR"
     gene            6607..6735
                     /locus_tag="VFQ96_06605"
     CDS             6607..6735
                     /locus_tag="VFQ96_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257493.1"
                     /translation="MTMNTAGNTATTKHHSQPNGRIKLIDTAASTDPTGQPDCTKE"
     gene            7511..7858
                     /locus_tag="VFQ96_06610"
                     /pseudo
     CDS             7511..7858
                     /locus_tag="VFQ96_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(7879..>8135)
                     /locus_tag="VFQ96_06615"
     CDS             complement(7879..>8135)
                     /locus_tag="VFQ96_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257494.1"
                     /translation="MPHTHDLVTVLRRHGFAAVVSGSGPSVLVLCDDPARRLAAAELA
                     VSHGEGHWEARLPAVDFKGATIDTQNAPQAVGAGLLGRSD"
BASE COUNT         1298 a         2920 c         2694 g         1223 t
ORIGIN      
        1 ggcgccgccg ggccgcacca gcacggccgc gcgccgggcg aggacgcgga tgacggccag
       61 gccgtcgggc ccgccgtaca gcgcgcgcgg cgggtcacgc cgggcctccg gctccggcgg
      121 ccgctcgcgc gccggcacgt agggagggtt gctgacgacg acgtcggccg cgccgtcaac
      181 acccggcagg gccgcccgaa ggtcgccgta cacgaggcgc acgccgggcg cgatgcgggc
      241 gatattgcgg cgggcgaagg agcaggccgc ccccgacgcc tcgacggcga cgacccgtgc
      301 gtcgggcacc tccacggcga gggagagtgc gatcgccccc gtcccggtgc cgagatccac
      361 gccgagggcg ccgggccccg ccgctcgcag caggtcgatg gcaagctgcg cgaccatctc
      421 cgtctcgggc cgcggcacga agacgccagg gccgacgtcg aggacgagcg tgcggaacgc
      481 ggcagtgccg acgatatgct gcagcggttc gtgcccggcc cgcctttcga ccattgccgc
      541 gtaacgcgcc gcctcgcgct cgctcaaagc cgcccccgcg atcgccatcg cctccacgcg
      601 gccgcgggag gcgcccagaa gatgcccggc caggagcacc gcgtcagtgc gcgccgacgc
      661 gatccccgcg gcccgcaggc ccccggtcgc cccggcgagc gcggccgccc atgtcggcca
      721 cgcccccgcg gcatctcgcc ccgccgcggg aaggtcgccc gacgccccgg gcgggcggcc
      781 cgcggcgttg gggcccgatg ccacgggctc ggaaacgttc atgcggctac cagaataggc
      841 tgtgcatgtc ggcggccgcg aggtgctcgc ccgacacgca tttcagcacg gcggcatccg
      901 atgagagggc ggacaatgac ccggatccac gcagacatca ccacggcggt cggcgggacg
      961 cccctcgtgc agctgaacca cctgacgaac gatgcgtcgg cgagggtcct cgccaagctg
     1021 gagttctaca accccgcggg cagcgtgaaa gaccggatcg gcgtcgccat ggtcgatgcg
     1081 gccgaggcgc aaggcacgct gcgcccgggc ggcaccatca tcgaggcgac gagcggcaac
     1141 acgggtatcg cgctcgcgat ggtcggcgcg gcgcgcggct accgaacgat catcgtgatg
     1201 cccgagaaga cgagcaagga gcgccgcatc ctgatccgtg cgtacggcgc cgaggtgatc
     1261 ctgacgggcc cgggcggcat ggtggaggcg aaccgcaagg tgcgggagat cctcgaggcc
     1321 gaccccgaag ccgtgtggct caaccagttc gagaacgccg tgaaccccgg catccatcac
     1381 gacacgacgg ggcaggagat ctgggacgac accgacggca aggtcgacgt cttcgtcgcc
     1441 ggcgtgggca cgggcggcac gctcaccggg gcggcccggc ttctcaaaga gaagaagccc
     1501 gcggtgcacg tcgtcgccgt cgagccggcc gaatccccgc tcctgacggg cggcgagccc
     1561 ggcccgcacc gcatctcggg aatcggtgcg aacttcatcc ctccggtgct cgaccgcgac
     1621 atctacgacg aggtcgccga cgtgccctcg agtgaggcgc tcgacatggc gcgcgcactt
     1681 gcctcacagg agggcatctt ctcgggcgtc tcgggcgggg ccgcctgcgc cgcggcgctc
     1741 gtgcaggccg ccaagccgga gaacgccggc aagacgatcg tcttcgtcgt tcccgatttc
     1801 ggcgaacggt atctctcgac gtcgttgtac cggcacctgg ccgactgacc ggccggtccc
     1861 caacagcgca cggcgtggac atgagcgttc tctcccgcat tcgcgaagac atcgagacgg
     1921 cgaaacgccg cgatccggcc gcccgcagcg gcacggagat cctgctcggc tattccgggc
     1981 tgcacgcgat ctgggcatac cgactcgcgc atcgttggtg gaaggcgccg ggcatgaagc
     2041 tgccggccag actcctgtcg cagctgacgc gctcggtgac gggcgtggag atccaccccg
     2101 gcgcccgcat cggccgcagg tttttcatcg accacggcat gggcatcgtc atcggcgaga
     2161 cggcgatcgt cggcgacgac gtgatgctct accacgacgt gaccctgggc gggcgcgggt
     2221 cgaggcccgg caaacgacac ccgaccctcg gcgacggcgt ggtcgtgggg gccggcgccc
     2281 aattgctcgg caacatcacg atcgggcccc ggtgccaggt tggggcgaac gcggtcgtcg
     2341 tgcgcgacgc gccggccgac tcggtgctgg tcggcatccc ggcccgcgtc ttgccgagac
     2401 gtgtcgccgg ccgcgaccga caagaccacg acccgaacga gtggatcgac cccacgttct
     2461 acgtcatctg accttcttgc gccgcggccg gcaacgcaac gatgcggccc ggtaggccgg
     2521 ccccggcggc gggccccgtc aggactcggc cgcgccggtg cccagctcgg ccaagcgcgc
     2581 ctcttcgtca gcccgcacac aggaggcgat gacggcgtcg agcgcgccgt ccatcacctg
     2641 gtcgagattg taggccttga acccggtgcg gtggtcggcg atgcgattct cgggaaagtt
     2701 gtaggtgcgg atccgttccg agcgatccat ggcgcgaatc tggctgtgcc tggcggcagc
     2761 gccggcagcc gcctgctcct cctgctggcg ggcgaggagg cgcgcgcgga ggacccgcat
     2821 cccggcctcc ttgttctgca actggctctt ctcgttctgc atcgagacga cgatgccggt
     2881 cggcaggtgt gtgatgcgca ccgccgaatc cgtcgtgttc accgactgcc cacccggccc
     2941 ggaggaacgg taaacgtcga tcttcaggtc ggcctggctt atctcgatct cgtcgggctc
     3001 gtcgacctcg ggaaagacga ggaccccggt cgtggaggtg tggatgcggc cctgcgactc
     3061 ggtgacgggc acccgctgca cccggtgcac gccgccctcg tatttcagat gcgcccagac
     3121 gccgccgtcg ggatcgttgt tcgacgactt gatggccaac tgcacgtctt tgtagccgcc
     3181 gaggtcgctt tccgtctggc ccaaaagctc cgccgtccag cccttcgacg ccgcgtaatg
     3241 cagatacatt cgcagcaggt ccccggcgaa cagggcgctc tcctcgccgc cctcgccgcc
     3301 cttgatctcc atgatgacgt cgcgcccgtc gtcggggtca cgcggcacga ggagacgccg
     3361 cagctcctcc tcggcgagag ccaagcgttc cgcaagaccc ggaatctcct ccgcgaacgt
     3421 cgcgtcttcg tcggcaagct cccgcgccgc ctgcaggtcg ccttgcagcg cgtgccaggc
     3481 gtcgtacgcg gccttcacgc gactcaactc ggcgtagcgc cggccgacct tgcgcgcccg
     3541 ggccgcgttg gcatgcaacg tcggatcggc cagctggcct tccagacgag cgtgctcgtc
     3601 gagcagcgtg tcaatcgact cgaacacggc cgcctcagcg tgcgtcgacg tggccggcgg
     3661 cgccgggcaa cgacttctgc atctgcatca gaaactcgac gttcgtcgag gtctctttga
     3721 tacggccgag caccgcctcg agcgcctgct gttgatcgag gccggccagg gcgcgacgca
     3781 gtttccacgt cacattcacc tcgtcggcac ccatcagaag ctcctcacgg cgggttccgg
     3841 aggcgctcac gtcgacggcg gggaagatcc gcttgtcggc cagctgccgg gaaaggcgca
     3901 gctccgagtt gccggtgccc ttgaactctt cgaagatcac ctcgtccatg cgcgagccgg
     3961 tttcgaccag ggcggaggcg aggatcgtca gcgagccgcc gtgctcaatg ttgcgcgccg
     4021 caccgaagaa ccgcttcggc gggtacagcg cggcggcgtc gaccccgccg gagagcaccc
     4081 tgccggaggt gggggccgtg atgttgtagg cacggcccag ccgggttatc gagtcgagca
     4141 gcacgaccac gtcgtgcccg agctcgacca gccgcttggc ccgttcgatc gccagctctg
     4201 cgaccgtcgt gtggtcttcg gccggccggt cgaacgtcga ggcgatgacc tcgccgttca
     4261 ccgtgcgctg catgtcggtg acttcttcgg gccgctcgtc gacgaccacg accatcagat
     4321 gcacctcggg gttgttcttg gcgatcgcgt tcgcgatctg ctgcatgacg atcgtcttgc
     4381 ccgccttggg cggcgcgacg atgagcccgc gctgtccctt gccgatcggg gcgacgaggt
     4441 cgatgacccg ctgcgtgacc tttccaggct ccgtctccat gcgcaggcgt tcctgcgggt
     4501 agagcggggt caggtcggcg aactgcacgc ggtgctgcgc ctcctcgacg ctctggccgt
     4561 tgatcgaatc caccttgacg agcgcgttgt acttctgcct gccggagcct tcgccctcgc
     4621 gaggctgacg caccgcgccg acgacggcat cgcctttgcg cagagcgtac ttgcgcacct
     4681 gcccgagaga gacgtacacg tcgctcgcgc cgggcaggta gccggtggta cgcacgaaag
     4741 cgtagttgtc gagcacgtcg agaatgccag cgatggggac gagcacgtcg tcttcgagga
     4801 tctcgggctc cgcctcctct ttactgccgc ggtgcttacg gtcccggccg cggccacggc
     4861 cacgctcccg gtgttcgccg ttgccgttct ccttctggct ctgctggccc tgacgaccac
     4921 gttgctgcct gcccgagtgc tccgcctcac cggcctcacc ggcctctgcg ccctcgcccg
     4981 aacgctcggc ggtgtcccgg ccggcgcctt gctgggcctc cgtgtgacga tgctcgccgt
     5041 tcttacggtt ccggctgcgt ccgcgcgtcg tccgctcgcg ccccgcctgc tccccgccgg
     5101 tgacgtgctc gtcggctccg ccttcccggc cctcggcacc ctcgccggcg tcggatgccg
     5161 ccgcaggcct accggccgcg tcgacctcga gccttccacg ccccctcgcc cgatccgttc
     5221 gcgccggctc tgccgcagcg ccgccctctg agactccgga cgcaccgagg tcgacgcccg
     5281 cacggccgcc gccggcgtga tccgccgcgg cgaccacggg tgcctggcct gccgccgacc
     5341 cgccgccgcg ggcacggcgc ccgccctgac gtgctttaga cgccaccgga ggcgcggctt
     5401 ccgccgccgg agggacctcg gccgccggag ggacctcggc cgccggctcc gcgggagcct
     5461 cggccgccgg cccggcctcg ggcatcggca gctggatgtc cgcgctcgtg gcgcggcgcg
     5521 gacgccggcc ggcaggagcc ggcgcggcac cgccacgcac ggcccggatc cgttcgacga
     5581 gctcgctctt gcgcagccgc gcggatccgg tgatgcccag ccgggcggcg acggcctgca
     5641 gttcggccac ccggagcccc ggcagcgcct cgtcggcggg ggtcgctgat gaatcgttga
     5701 cgttcgtcac gtgagagttt tccttccctc ccgcctcgga agaagaccgg gcggggactg
     5761 cgtgccgcgg gaaaatgcct ggtatcggaa cccgaccctg gccgtgtcgc agggaacgac
     5821 aacagaacgg cagcaatggg ttgtgcatac gacacgcggg ccagaaccgc acaccatggt
     5881 gcgtcacccg acgtgtccgg caccgagcct atcatccttc gcgatgcggc cgatctcggc
     5941 tcccggggcc cagaagacac gccgccagcg ggcagctgac gtgtctttct tcgacggccc
     6001 gccgtcgctt cgtgccgcca gacgaccact gagcccgccc gcgcatggtg gcgcgggcgg
     6061 gcgcgttgtg tcctgatggg cgccgtcaac cggccacgtg gcgcccccgc gggcgagcga
     6121 cgaccacctc tccgtcttgc atcaccagtt cgccccgcag gaacgtcatg acgggagcac
     6181 ccacgacctt ccgcccctcc cagaccgaat aatcgctttt gcctcggtaa cggtcgactc
     6241 acgaggtcgc cgaaccatcc gaagagaaga tagcccgcaa acaaacccgc gttagagacg
     6301 atgaggatca ggagcgcgtt gtccaaagtc aacgccttgc cgctcgtcgc tgtgagcaac
     6361 gaggtcccca agatgatgaa gcacagggtg cccagccaat tcagaaagaa cgatccgccg
     6421 aggacgagcg tcgcgcgcaa tgactcgcct tggaaaaccg ccgcgagcgg cgtcgcgacg
     6481 tcgtcgatcg tgatccccgc agacctcgcc acggcgtgcg ccgcctcgcg gtcgcccgag
     6541 cgaagcagtt tcgtcgcctc gcgccgcgcg atgaactgcg gcgtctcttt gaggtagcga
     6601 ccgacgatga caatgaatac tgccgggaac acggcgacga cgaaacacca ttcccaaccc
     6661 aacggcagga tcaagctgat cgatactgcc gcgagcactg acccgaccgg ccagccagac
     6721 tgcaccaagg aatagatgaa ccctctccgg cgcaccttgg cggggtccga gtgcgaatgt
     6781 gccatcagct cgctgaggta aacagcattc accgtctgct cggcaaagcc tagccctgca
     6841 acggaacgga agagaatgag gagcacgaca ccgaagccgg caaagatacc gataagcaac
     6901 cccgccgtcg cggtaagcac ggacgccacc gccgcaccga tcactgctat atatattccc
     6961 cgacgacggc ccacgcggtc gacgataggg ccgatagcca tggcaacaat cgcggtggcg
     7021 gaatccggca atttcactcg ggcggcaaga agttgtttcg tcgcccaatc ggcactcagc
     7081 cgtcagatca aggctctcga agacgaatta ggtgtcaagc ttttcgctcg cacgagtaga
     7141 agcgtcatgc tcactcgggc aggcgaagcc ttcgtgcggg ccgcccggac gacggtggat
     7201 tccgcgagtc gcgccgcaga ggaagcactc tgcgcagcgc agacggtgtc gggaagtctc
     7261 gtgatcggcg tgaacccgac attgacagca atggatatct cgacactgct acgagattat
     7321 tgcgagcaat accccggcgt ccgaatagcc ctcaagtcgg aaaacagcgg tcgcctcatg
     7381 aacaaggttc gcaaaggcac gatcgacatc tcgtttatcg cgctcgaggc ctcccacagc
     7441 ccgcagggcg tcttattccg agagataggc cgcgaagccc ctcgccgttc ttttctgacc
     7501 cggtcatcca ctagacgcct cacgctctct gactctgaac gatatcgcgc gcgaaacgtt
     7561 cgtcgacttc ccagagaaca cgacgggccg ggcgcaagcc gacatcgcct tctccaaggc
     7621 caagatccgc cgaaacgtct cgttcgaggt ctcggacgtc gccttgatgg cccagctgat
     7681 ccgcggcagg ttggcgatcg cgctcctggc ccagccgatc gccgaacggc tcgacgcgtt
     7741 acgcaccgtg aagctgcacg cagctcccga gcgggtgcaa tatatcgcgt ggtccgcctt
     7801 ccacacctcg actgcggccc gggccttctt gcgaacggtg gaatcccatt cccgatgaac
     7861 gggccggcac cgcgacactc agtcggagcg gccgagaagg cctgcgccca cggcctgcgg
     7921 cgcattctgc gtgtcgatcg tggcaccctt gaaatcgacg gccggcagcc gggcctccca
     7981 atgcccctcc ccgtgcgaga ccgcgagttc cgccgcggcc aggcgcctgg ccggatcatc
     8041 gcacagcacc aggacggagg gcccggaacc cgagacgacg gccgcgaagc cgtgccgacg
     8101 caacacggtg acgaggtcat gggtgtgggg catcg
//
LOCUS       DASTNL010000208         5567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1265702, whole genome shotgun sequence.
ACCESSION   DASTNL010000208 DASTNL010000000
VERSION     DASTNL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265702"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..124
                     /locus_tag="VFQ96_06620"
     CDS             11..124
                     /locus_tag="VFQ96_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU0257495.1"
                     /translation="MLRCTLSVDHRAVDGALAARWLAAFQRRIERPLSILA"
     gene            complement(85..1164)
                     /locus_tag="VFQ96_06625"
     CDS             complement(85..1164)
                     /locus_tag="VFQ96_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257496.1"
                     /translation="MSRRVTIADVAVRAGVHKATVSRALNKMTEHQVNPATARRVRKA
                     AEELGYVANAVARGLRTNSSMTVGVIIPDLTNPLFPPMVRGIEHYLAPHGYTALIANT
                     DGDDDLERAAFDSLSGRRVDGFIIATGRMHHELIADAYHRGVHVVMLNRGSVDVPYPL
                     VTGDDAAGVTAAVEHLVELGHRDLVHLAGPENFTTSAVRQDAFLAAVRRHADVAGRLV
                     PAPALTIRAGLECMNDMLLHGDAPTAIVAGNDLLAVGILRSLRQHGLHCPDDVSVIGF
                     NDMDSTEDLNPPLTTVRVPHFEMGTEAARLLIDDLAADRQRSVTVSLPASLVVRESTA
                     APRAGALASADTFQARIESGLSMRR"
     gene            1435..1572
                     /locus_tag="VFQ96_06630"
     CDS             1435..1572
                     /locus_tag="VFQ96_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257497.1"
                     /translation="MSGWGAGYPGMPAEAVKARRQAEAVKATQQQNAEADEEGDGRTA
                     L"
     gene            1855..3729
                     /gene="dnaK"
                     /locus_tag="VFQ96_06635"
     CDS             1855..3729
                     /gene="dnaK"
                     /locus_tag="VFQ96_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU0257498.1"
                     /translation="MSRAVGIDLGTTNSVVAVLEGGEPTVIANAEGFRTTPSVVAFTK
                     DGEVLVGETAKRQAVTNVDRTISSVKRHMGTDWKADIDGKKYTPQEISARILGKLKRD
                     AEQYLGDTVTDAVVTVPAYFNDSERQATKEAGEIAGLNVLRIINEPTAAALAYGLDKG
                     KEDELILVFDLGGGTFDVSLLEVGKDDDFSTIQVRATAGDNRLGGDDWDQRVVDHLIK
                     KFKETSGVDVSGDKIALQRLKEAAEQAKKELSSSMSTSVQLPYLSLTQNGPANLDENL
                     TRAQFEQMTEDLLARTKKPFTDVIAEAGIKVSDISHVVLVGGSTRMPAVSELVKTLTG
                     GKEPNKGVNPDEVVAVGATIQAGVLKGERKDVLLIDVTPLSLGIETKGGIMTKLIERN
                     TAIPTKRSETFTTADDNQPSVAIQVFQGEREFTRDNKNLGTFELTGIAPAPRGVPQVE
                     VTFDIDANGIVHVSAKDKGTGKEQSMTITGGSSLPKEDIDRMVHEAEENAAADKKRRE
                     EAETRNSAEQLSYSIEKLIKENADKLPDNVKNEVQADVDALKSALAGTDEDAVKTAFE
                     KLTQSQTKLGEAIYKSGQAEAAAGAAGSGAQGEAPEAGSAQDDDDVVDAEVVDDEEQK
                     "
     gene            3732..4469
                     /locus_tag="VFQ96_06640"
     CDS             3732..4469
                     /locus_tag="VFQ96_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU0257499.1"
                     /translation="MARKDESPQEPGRPHDGDRQDWHEPVVHDKRRIDPVTGKVRHPE
                     GEAPSGQGAAAGPGEGSGVGGEGSVTDAELDALLNESGAGQPGAASEADAPLVEAKRL
                     AAERLADLQRVTAEFANYRRRTENNRVLERERTVGDVASALLPVLDDLDRAEKHGDLA
                     DEKSAFATIAAKLRGVAERLGLTKFGQAGDAFDHNVHDALFQKPDAEVSVDTVAEVVE
                     TGYTLGDTLVRAAKVVVATPQGPAAGE"
     gene            4521..5504
                     /locus_tag="VFQ96_06645"
     CDS             4521..5504
                     /locus_tag="VFQ96_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU0257500.1"
                     /translation="MASQDWLDKDFYKVLGVSKDVGEAELKKAYRKLARKYHPDSNPG
                     DAAAEGRFKEISEAYAVLSDKEQRQEYDQIRAMGSGARFTAPGAGSGSGGFEDVFGGM
                     FGRGAQYQGAGFGRGGYEDLLGNIFGSGRFGTPSGGFRGGGGPTKGGNVEATTTLDFL
                     TATKGDTVQLQTGEGRGITVKIPAGVADGQKIRVRGKGLRSPDGGPPGDIVLTVTVRK
                     HPVFERDGLNLRVTVPVTFTEAALGATIEVPTLGGPAVRLRVAPGTPSGRVLRVKGRG
                     VATKKATGDLLAEVQVVVPSHLPKDAEEALRAFEAALPAENPRDDLLARAR"
BASE COUNT         1004 a         1790 c         1988 g          785 t
ORIGIN      
        1 ggtggcgacg gtgctgcgct gcactctctc ggtcgatcac cgcgcggtgg atggcgcgct
       61 cgccgcccgt tggctcgccg cgtttcagcg ccgcatcgag aggccgcttt cgatcctcgc
      121 ctgaaaggtg tctgcggagg cgagggcgcc cgcccgtggc gcggccgtcg actctcgcac
      181 gacgaggctc gccggcaggc tcaccgtcac agagcgctgc cggtcggcgg cgaggtcgtc
      241 gatgagcaga cgggcggcct cggtgcccat ctcgaaatga ggcacccgca cggttgtgag
      301 cggcgggttg aggtcttcgg tcgaatccat gtcgttgaag cctatgaccg agacgtcgtc
      361 ggggcagtgc agcccgtgct ggcgcaggga acgcaggata ccgacggcga gcaggtcgtt
      421 gccggcgacg atcgcggtgg gcgcgtcgcc gtgcagcagc atgtcgttca tgcactcgag
      481 cccggcgcgg atcgtcagag cgggggcggg cacgaggcgc cccgcgacat ccgcgtgtcg
      541 acgcacggcg gcgaggaacg cgtcttgccg aacggcgctc gtcgtgaagt tctccggccc
      601 ggccaggtgc acgaggtcgc gatggccgag ttcgacgagg tgctccacgg cggcggtcac
      661 ccccgccgcg tcgtcgcccg tcacgagcgg gtatggtacg tcgacgctgc cgcggttcag
      721 catgacgacg tgcacgccgc ggtggtatgc gtcggcgatg agctcgtgat gcatccggcc
      781 ggtcgcgatg atgaacccgt cgacccggcg ccccgacagg gaatcgaacg cggcacgctc
      841 caggtcgtcg tcgccgtcgg tgttcgcgat gagggcggtg tagccgtgcg gcgccaggta
      901 gtgctcgatg ccgcgcacca tggggggaaa gagcgggttc gtgagatcgg ggatgatcac
      961 gccgaccgtc atggacgagt tggtgcgaag ccctcgcgcc acggcgttgg cgacgtagcc
     1021 gagctcctcc gccgctttgc gcacccgccg tgcggtcgcg gggttgacct gatgttcggt
     1081 catcttgttg agcgcacgcg acaccgtcgc cttatgcact ccggcccgca ccgccacgtc
     1141 ggctatcgtc actcgtcgag acacgttctc agcgtaccaa ccggttgcag tacaggttgc
     1201 gggtgttgca gggatcgcat cgtctgcccc gcaaggagac gggggtgtgg tgcccataat
     1261 cgaaggagat cgattgcggt aattcgaccc gaatcgctgg cttagccccg ggtcgtcact
     1321 ttatactctc cttattaaca accgggctga atcctgagga caggggttgc gccacggttc
     1381 gtctgtggcg cggtggcccg ttgtgggggt gtcgcgcgga agagaggtca tgagatgagt
     1441 ggatggggtg cggggtatcc cggtatgccg gccgaagcgg tgaaggcccg ccggcaggcc
     1501 gaagcggtga aggcgacgca gcagcagaac gccgaggccg acgaggaggg tgacgggcgt
     1561 accgcgctgt gatgcgtcct gcgcagtgat ccgccttgcc gagtgacgtg tcctgcgcgg
     1621 tgagcggctg tcgacgggcc ggtgctgcgc cgcggcggcc gtgcgtcttc gggcgtgcgg
     1681 tcgccgttcg tcgtgttcgg gaggggaatg ttcgccgcga aatcaaggtt gatatgaatg
     1741 cgctcaagtt tggtcggccg gtgttgacag caagctattg gccggtaaac ttgagccgaa
     1801 ggcgctcaac tagcaccacg gcttagatcc ccagaaatcc ggaaaggcac agacatgtca
     1861 cgagcagtcg gaatcgacct cggaaccacc aactccgtcg tcgcggtcct cgaaggcggc
     1921 gaacccaccg tcatcgccaa cgccgaaggc ttccgcacga ccccctccgt cgtcgcgttc
     1981 accaaagacg gcgaggttct agtcggcgag accgcgaaaa ggcaggccgt caccaacgtc
     2041 gaccgcacca tttcgagcgt caagcggcac atgggcaccg actggaaggc agacatcgac
     2101 ggcaagaagt acacgccgca agagatctcg gcccgcatcc tcggcaagct gaagcgcgac
     2161 gccgagcagt acctcggcga caccgtcacc gatgcggtcg tgaccgtgcc cgcgtatttc
     2221 aacgattctg agcgccaggc caccaaggag gcgggcgaga tcgccggcct gaacgtcttg
     2281 cgcatcatca acgagccgac cgcggcggcg ctggcctacg ggctcgacaa gggcaaagaa
     2341 gacgagctca tcctggtgtt cgacctgggc ggcggcacct tcgacgtctc gctgctcgag
     2401 gtgggcaaag acgacgactt ctccacgatt caggtgcgcg ccaccgccgg cgacaaccgg
     2461 ctcggcggcg acgactggga tcaacgggtc gtcgatcacc tgatcaagaa gttcaaggag
     2521 acctccggcg tcgacgtctc cggggacaag atcgccctgc agaggctcaa ggaggcggct
     2581 gagcaggcca agaaagagct ctcctccagc atgtcgacga gcgtgcagct gccttacctc
     2641 tcgctcacgc agaacgggcc ggcgaacctc gacgagaacc tcacccgtgc ccagttcgag
     2701 cagatgaccg aagacctttt ggcccgcacg aagaagccct tcaccgacgt catcgccgag
     2761 gccggcatca aggtctccga catctcgcac gtcgtgttgg tgggcggctc gacccgcatg
     2821 ccggccgtca gcgagctcgt gaagaccttg accggcggca aggagcctaa taagggtgtg
     2881 aaccccgacg aggtcgtcgc cgtcggcgcc acgatccagg ccggcgtctt gaagggcgaa
     2941 cgcaaagacg tgctgctcat cgacgtgacg cctctgagcc tcggcatcga gaccaagggc
     3001 ggcatcatga ccaagctcat cgagcgcaac acggccatcc cgaccaagcg cagcgagacc
     3061 ttcacgacgg ccgacgacaa ccagccctcc gtcgccatcc aggtgttcca gggcgagcgg
     3121 gagttcacgc gcgacaacaa gaacctcggc accttcgagc tcaccggtat cgctcccgcc
     3181 ccgcgcggcg tgccgcaggt cgaggtgacc ttcgacatcg acgcgaacgg catcgtgcac
     3241 gtctccgcga aagacaaggg caccggcaag gagcagtcga tgaccatcac cggagggtcg
     3301 tcgctgccga aggaagacat cgaccgcatg gtgcacgagg cggaggagaa cgctgccgct
     3361 gacaagaagc gccgcgaaga ggccgagacc cgcaacagcg ccgagcagct gtcgtactcg
     3421 atcgagaagc tcatcaagga gaacgccgac aagctgcccg acaacgtgaa gaacgaggtt
     3481 caggcagacg tcgacgcgct gaagtccgcg ctcgccggaa ccgatgagga cgccgtcaag
     3541 acggcgttcg agaagctcac gcagagccag accaagctgg gcgaggcgat ctacaagtcg
     3601 ggtcaggccg aggccgccgc aggtgccgcc ggttccggcg cacagggcga ggcgcccgaa
     3661 gccggctccg cccaagacga tgacgacgtg gtagacgccg aggtcgtcga cgacgaggag
     3721 cagaagtaac catggcccgc aaagacgagt ccccccagga gccgggccgc ccgcatgacg
     3781 gcgaccggca agactggcac gagcccgtcg tgcacgacaa acggcgcatc gacccggtga
     3841 ccggcaaggt tcgccacccg gagggggagg ccccctccgg gcagggcgcc gcggccggcc
     3901 caggcgaagg ctcgggtgtg gggggtgagg gcagcgtcac cgacgccgag ctcgacgcgc
     3961 ttctgaacga gtcgggcgcg ggccagccgg gtgccgcctc ggaggcggat gccccgcttg
     4021 tcgaggccaa gcgtctggcc gccgaacgcc tcgccgacct gcaacgcgtc accgccgagt
     4081 tcgccaacta tcgcaggcgt accgaaaaca accgtgtgct cgaacgtgag cgcaccgtcg
     4141 gcgacgtggc ctccgccttg ctgccggtgc tcgacgacct cgaccgagcc gagaagcacg
     4201 gcgacctagc cgacgagaag agcgcgttcg cgacgatcgc cgcgaagctg cgcggggtcg
     4261 ccgagcggct cgggctgacg aagttcgggc aggccggcga cgcgttcgat cacaacgtgc
     4321 acgacgcgct cttccagaag cccgacgccg aggtgagcgt cgacacggtc gccgaggtgg
     4381 tcgagacggg ctacacgctc ggcgacacct tggtgcgtgc ggcgaaggtc gtcgtcgcga
     4441 ccccgcaggg cccggccgca ggggagtgac gcgaccggtg cggggtgcga cgccgatgag
     4501 gcgggagagg gaggtgccgg ttggcaagcc aggactggct cgacaaggac ttctataagg
     4561 ttctcggcgt ctcgaaagac gtcggtgagg ccgagttgaa aaaggcttac cgcaagctag
     4621 cccgcaaata ccatccagat tccaacccgg gcgatgccgc ggctgagggg cggttcaagg
     4681 agatcagcga ggcgtacgcc gtgctcagcg acaaggagca acggcaagag tacgaccaga
     4741 ttcgcgcgat gggctcgggg gcgcgcttca ccgcaccggg ggcgggctcg ggctccggcg
     4801 gcttcgaaga cgtcttcggc ggcatgttcg gccggggcgc gcagtatcaa ggcgccgggt
     4861 tcggccgggg cggttacgaa gaccttctgg gcaacatctt cggcagcggg cgcttcggca
     4921 cccccagcgg cggcttccgc ggcggcggcg ggcccacaaa gggcggcaac gtcgaggcga
     4981 cgacgaccct cgactttctg acggccacca agggcgacac cgtgcagctg cagaccggcg
     5041 aggggcgcgg aataaccgtg aagatcccgg cgggggtggc cgacgggcag aagatccggg
     5101 tgcgcggcaa gggtctgcgc agtcctgacg gggggccgcc cggcgacatc gtgctcaccg
     5161 tcacggtgcg caagcatccg gtgttcgaac gcgacggcct gaacctgcgg gtcaccgtgc
     5221 cggtgacgtt taccgaggcg gccctcggcg cgacgatcga ggtgccgacg ctcggcggcc
     5281 cggccgtgcg gctgcgggtc gccccaggca cgccgtccgg ccgcgtcttg cgggtgaagg
     5341 ggcgcggggt cgcgacgaag aaggcgaccg gcgacctgct tgccgaggtg caggtggtcg
     5401 tgccgtcaca cctgcccaaa gatgcggagg aggcgctgcg cgcgttcgag gccgcgttgc
     5461 cggccgagaa cccgcgcgac gacctgctgg ccagggcccg ataggcaggc ggtggcgatc
     5521 gtggtgcgca gacggggtgg cggggcgggc gcccccgccc accggcc
//
LOCUS       DASTNL010000209         5842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_711101, whole genome shotgun sequence.
ACCESSION   DASTNL010000209 DASTNL010000000
VERSION     DASTNL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711101"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="pgi"
                     /locus_tag="VFQ96_06650"
     CDS             <1..973
                     /gene="pgi"
                     /locus_tag="VFQ96_06650"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU0257501.1"
                     /translation="ALGESEADRNAAVARHFVAVSTALDRVAQFGIDPANAFGFWDWV
                     GGRYSVDSAIGLSLAVTIGPDNFRELLAGFHAVDLHVATTPLARNVPVLMGLINVWYT
                     NFLGAQSHAVLPYAQQLNRFPAYLQQLTMESNGKSVRWDGSPVTTDTGEVFWGEPGTN
                     GQHAFYQLIHQGTRLIPADFIAFVKPAYPLTDGDRDVHGLLLANFLAQTKALAFGKTA
                     AEVEKEGTTGALVAARTFPGNRPTTSIFAPALTPRVLGELVALYEHIVFTEGTVWGIN
                     SFDQWGVELGKQLALEIAPAIEGNAEAVADQDASTRALLAYYRSQQG"
     gene            1051..1842
                     /locus_tag="VFQ96_06655"
     CDS             1051..1842
                     /locus_tag="VFQ96_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0257502.1"
                     /translation="MRRRKSRRIAAFVAIGLLTGLIAGLFGVGGGAIIVPLLVITAGF
                     DQRLAAGTSLAAIVPLSIAGVASYAARGDLAPLAALILAVGAVVGAQLGTWLLSRIPT
                     TVLRWAFIVFLATVIVGLFVVIPSRDAHFVVTWPAGIALAGTGLVTGVLSGLLGIGGG
                     SIMVPVLMLGFGTSDLVAKGTSLLVIVPTSISGTLGNMRRSNVDLPAAAAVGLAACVT
                     TPIGAAIAALLNPFAGDVLFALFLIISATRMALKAARGGRAKARA"
     gene            2032..2541
                     /locus_tag="VFQ96_06660"
     CDS             2032..2541
                     /locus_tag="VFQ96_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257503.1"
                     /translation="MVSKTAEEDGRFDTMAALVAAYRADLDASRDDAWWGRADYAVKM
                     GHGTAHLQPSRPDVTDDGTAQKSWRVRFDTDEGSLERRFTDDRLPTPAEAERRLAALA
                     RQALPSRHPERDRVVARLRELAGERHKIDAEARRIALEAVAHGVKKIAVADAARISRV
                     TLDRWIRGR"
     gene            2643..3464
                     /locus_tag="VFQ96_06665"
     CDS             2643..3464
                     /locus_tag="VFQ96_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0257504.1"
                     /translation="MARSAARRPIEVEPGDIPLAARRVEHVQGHWLLARLGKRVLRPG
                     GLRLTKPLLREARVTGAHVVEFAPGMGRTATIVLSHDPADYVGVDADPKAAAVIDRLV
                     SAKGRAIAARADASGLPDASADIVLAEGVLTIQSEEVKRSIVAEAARLLRPGGRYVLH
                     ELSLKDAAAGKADGIRDALAHAMHVHARPKTIGEWRRLVTECGLEVRFVHTSPLALLE
                     PQRVVADEGFVQAMRFFRNVLRDGPTRRRVFTMARTMRAHKPFLQAVGIVAVKPG"
     gene            complement(3544..4587)
                     /locus_tag="VFQ96_06670"
     CDS             complement(3544..4587)
                     /locus_tag="VFQ96_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257505.1"
                     /translation="MPAQSNGGSHPATRRAKRPTLRDVAALAGVSIATASKALNGRGA
                     VHPDTRDRVLVAAEEVAFTPNRLARNLLSGRSGTIGLLTNDLIGRFSIPILMGAEDAS
                     GTGEMSVLLCDARGDAIREAHQLKTLLSRRVDGLIVVGSDTDPRPSVGEKIPVPVVYA
                     YAPSTSPEDCSLVPDNIGGGRQVTQHLLDIGRTRIAHITGPADFLAAADRAQGVAEAL
                     GAAGLPLVGDRPLYGAWTEAWGRTAADTILDRFPDTDAIVCGSDQVARGVLDALHERD
                     LDIPGDVAVTGFDNWDVFATGSRPRLTTVDMDLELLGRLAAERLIRAIDQPLDAGITT
                     LPCRVVVRESTAG"
     gene            4747..>5842
                     /locus_tag="VFQ96_06675"
     CDS             4747..>5842
                     /locus_tag="VFQ96_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0257506.1"
                     /translation="MRSITRHRRKITALAALAAAAALALSGCSGGGSNGSAGSTSGSG
                     GSSSKTLTVWHYFSTPTQVKLMTDEAKLFEGENPGVTVKNVYVPYNNMDSKLITAAGS
                     QTGPDVVVFNGGDADNIENAGALLDISKQWSNYADSSQFPASAIHKLDGKVYSVQGYV
                     NLLGLWYNADILKKIGVQPPTTMGELNSDMAKAVKAGFGGITLSGQPQSQGEFQAYPW
                     LTEAGFSYANPSVKTLAAGLTTAQQWVKKGYLTGEASTWDQTVPFQKWAAGKIAFAEN
                     GNWQIATAKQTAKFTYGVVPLPLGANGTKVYLGGEAEGIGRYSKNPSLAWKYLEATFF
                     SKKGQLIALKDEGNIPSRKDAAADPQVSGNK"
BASE COUNT          906 a         1972 c         2031 g          933 t
ORIGIN      
        1 cgcgctcggc gagagtgagg ccgatcggaa cgccgccgtc gcgcggcact tcgtcgcggt
       61 ctccaccgcg ctcgaccgcg tggcgcagtt cggcatcgac ccggcgaacg cgttcgggtt
      121 ctgggattgg gtcggcggcc gctactccgt cgactccgcc atcgggctga gcctcgccgt
      181 gacgatcggg cccgacaact tccgtgagct tctggccggg tttcacgccg tcgacctgca
      241 tgtcgccacc acgccgctgg cccgcaacgt gccggtgctc atgggcctca tcaacgtctg
      301 gtacacgaac tttctcggcg cgcagtcgca cgctgtgttg ccgtatgccc agcagctcaa
      361 ccgctttcct gcctacctgc agcagctcac tatggagtcg aacggcaagt cggtgcggtg
      421 ggacggctcg ccggtcacga ccgacaccgg cgaggtcttc tggggggagc cgggaacgaa
      481 cgggcagcac gcgttctacc agctcatcca ccagggcacc aggctgatcc cggccgactt
      541 catcgcgttc gtgaagcccg cgtacccgct caccgacggc gaccgcgacg tgcacggcct
      601 gctcctcgcg aacttcctcg cgcagaccaa ggcgctcgcg ttcggcaaga cggcggccga
      661 ggtagagaaa gagggcacca ccggcgccct cgtcgcggcc cgtacgttcc ccggcaaccg
      721 gccgacgacc tctatcttcg cgccggcgtt gacgccccgc gtcttgggtg agctcgtcgc
      781 actgtacgag cacatcgtct tcaccgaggg cacggtgtgg ggcatcaact cgttcgacca
      841 gtggggggtc gagctcggca agcagctggc gctcgagatc gcgcccgcga tcgagggcaa
      901 cgcggaggcc gtcgccgacc aagacgcctc cacccgggcc ctcctcgcct actaccggtc
      961 tcagcagggc tagcgacggc cggggagggg ctgggccgga aacgcccgcc ggccgacgaa
     1021 ccggcgagtg acgcggccgc cggcggggcg gtgcgccggc gaaagtcacg gcgcatcgcc
     1081 gcgttcgtcg cgatcgggct tctgaccggg ctgatcgccg ggctcttcgg ggtcggcggc
     1141 ggggcgatca tcgtgccgct gctcgtcatc acggccgggt tcgaccagcg cctggccgcc
     1201 ggaacgtcgc tggcggcgat cgtgccgctc tccatcgccg gcgtcgcctc ctacgccgcc
     1261 cgcggcgacc tcgcgccgct ggccgccttg atccttgcgg tcggcgcggt cgtgggggcc
     1321 cagctcggca cctggctgct ctcgcgcatc cccacgacgg ttttacggtg ggcgttcatc
     1381 gtcttcctcg ccacggtgat tgtgggcctc ttcgtcgtca tcccctcccg tgacgcgcat
     1441 tttgtcgtca cctggccggc gggtatcgcg ttggcgggca cggggctcgt cacgggggtg
     1501 ctctcgggcc tcttgggcat cggcggcggc agcatcatgg tgcccgtgct gatgctcggc
     1561 ttcggcacga gcgacctcgt cgccaagggc acttcgttgc tcgtcatcgt gccgacctcg
     1621 atctccggca cgctcggcaa catgcgacgc tcgaacgtcg acctgccggc cgcggcggcg
     1681 gtcgggctcg ccgcctgcgt cacgaccccg atcggcgccg cgatagccgc tcttctcaat
     1741 ccgttcgccg gcgacgtgct cttcgccctg ttcctcatca tctcggccac gcggatggcg
     1801 ctgaaggccg cccgtggcgg gcgcgccaag gcccgcgcct gaaccgcgtc ggcgcctgcg
     1861 gggtgatcgc cttctgtccc tatgtatcaa agtttacata tcggtgtatc attagataca
     1921 tcatggcggg gcgtctcgcg gcaggggcgc tcagcgaacg gtgacggccc gacgggtgca
     1981 gcctcgggcc ataagacggg cgggcgaacg cgccgcccgg gggtgagagc gatggtttcg
     2041 aagacggcag aagaagacgg gcggttcgac acgatggcgg ctctggtggc cgcgtaccgc
     2101 gccgacctcg acgcgagccg cgacgacgcc tggtgggggc gagccgacta cgcggtgaag
     2161 atggggcatg gcacggcgca cctgcagccc agtcgcccag acgtgaccga tgacggcacg
     2221 gcgcagaaga gctggcgggt tcgtttcgac acagatgagg gctcgctgga gcgtcggttc
     2281 accgatgacc ggctgcccac gccggccgag gcggagcgtc gactcgccgc gctggcccgg
     2341 caggccctgc cgtccaggca ccccgaacgc gaccgggtcg tggcaaggct gcgcgagctc
     2401 gcgggtgagc ggcacaagat cgatgcggag gcgcggcgca ttgccctcga ggcggtcgcg
     2461 cacggggtga agaagatcgc ggtggccgac gcggcccgca tcagccgggt gacgctcgat
     2521 cgctggatcc gagggcgttg agcgcgaacg ggcggtgaaa aacccggcct gacgcggatg
     2581 ccttggctgg gacatcgatt cgtgacagca tcggagacgg ggcgtcgtca cggaaggaca
     2641 ctatggccag gtctgccgcc cgccgtccca tcgaggtgga gcccggcgac attccgctcg
     2701 ccgcccggcg tgtagagcat gtgcagggcc attggctgct cgcccggctc ggcaagcgcg
     2761 tgttgcggcc gggcggcctt cggctgacta agccgctctt gcgcgaggcg cgggtcaccg
     2821 gcgcgcacgt ggtcgagttc gcgccgggta tggggcgcac cgccacgatc gtgcttagcc
     2881 acgacccggc ggattacgtg ggcgtcgacg ccgacccgaa ggccgcggcg gtcatcgacc
     2941 ggctggtgag cgccaagggc cgggccatcg ccgcccgcgc cgatgcctcc gggctgcccg
     3001 acgcctcggc cgacatcgtg ttggccgaag gcgtgctgac catccagtct gaagaggtca
     3061 agcgttcgat cgtcgccgag gcggcgcggc tcttgcggcc cggcgggcgc tacgtgctgc
     3121 acgagctgtc gctgaaagac gcggcggcgg gcaaggccga cggcatccgt gacgctctgg
     3181 cccacgccat gcacgtgcac gcccggccga agaccatcgg cgaatggcgc cggctcgtga
     3241 ccgagtgtgg tcttgaggtg cgtttcgtgc acacgtcgcc cctggctctc ctcgagccgc
     3301 agcgtgtcgt cgccgacgag gggttcgtgc aagcgatgag gttcttccgc aacgtcttac
     3361 gcgacgggcc cacccgcagg cgcgtcttca cgatggcccg caccatgcgc gcgcacaagc
     3421 cgttcctgca ggcggtcggc atcgtcgccg tcaagccggg ctgaacgagg tgccccgacc
     3481 cgggcggcga ggacgcctac cggccggtgg cctccccgcc cgtgctccgc gatgccgccc
     3541 gggtcagccg gccgtggatt cgcggaccac gactcggcag gggagcgtgg ttatgccggc
     3601 gtcgagcggc tggtctatcg cccgtatcag gcgttccgcg gccagccggc caagcagttc
     3661 gagatccatg tccaccgtcg tcagccgggg gcgtgacccg gtggcgaaga cgtcccagtt
     3721 gtcgaagccg gtgaccgcga catctcccgg gatgtcgagg tcccgttcgt ggagggcgtc
     3781 gagcacccct cgggcgacct ggtcgctgcc gcacacgatc gcgtcggtgt cagggaaccg
     3841 gtcgagaatc gtgtctgcgg cggttctgcc ccacgcctcc gtccaggcgc cgtaaagagg
     3901 cctgtcgccg accaggggca ggcccgcggc acccaaggcc tcggccaccc cttgggcacg
     3961 atctgcggcg gccaggaaat cggccgggcc ggtgatgtgg gcgatgcgcg tgcggccaat
     4021 gtcgagcagg tgttgggtga cctgcctgcc tccgccgatg ttgtcgggca cgagggagca
     4081 gtcttcgggg ctggtcgagg gcgcgtaggc gtagacgacg ggcaccggga tcttctcccc
     4141 cacggagggg cggggatcgg tgtcgctgcc gacgacgatc agcccgtcga cgcgccggct
     4201 gagcaaggtc ttcagctggt gtgcctcgcg gatcgcgtcg ccgcgggcgt cgcagagcaa
     4261 gacagacatc tcgccggttc ccgaggcgtc ttcggccccc atgagaatcg ggatgctgaa
     4321 acggcctatc aaatcgttgg tcaaaagccc gatcgtgccg cttcggccgg aaagcaggtt
     4381 gcgcgcgagc cggttcggcg tgaaagcgac ctcttctgcc gcgacgagca cgcgatcgcg
     4441 ggtgtcgggg tgcacggcgc cgcggccgtt caacgccttc gatgcggtcg cgatcgacac
     4501 ccctgccagg gccgcgacgt ctcgcaaggt aggcctcttc gccctgcgcg tggcggggtg
     4561 tgacccgccg ttcgactgtg caggcaccgc cgcctccccc ttttccgtaa accatatctt
     4621 ctcatgcggt gtattcaggt ctcttagccc gtgctagcct aaggaaaacc ttttcggaat
     4681 gtttctcttc aatccattca gtggctccga aaagttccca tgaccaaggg agttcaggag
     4741 gaaaaaatga gatcgatcac acggcaccgc cgcaagatca cggcattggc ggcgctcgcg
     4801 gctgccgccg cgttggcctt gagcggctgt tcgggagggg gctcgaacgg ctccgcgggt
     4861 tcgacgagcg gttccggcgg ctcatcgagc aagacgctca cggtgtggca ctacttcagc
     4921 acgccgacac aggtgaagct gatgaccgac gaggccaagc tgttcgaggg tgaaaacccc
     4981 ggcgtcacgg tcaagaacgt ctatgtcccg tacaacaaca tggattccaa gctcatcacc
     5041 gcagccggca gtcagaccgg ccccgatgtc gtcgtcttca acggcggaga cgcggacaac
     5101 atcgagaatg ccggcgcgct gctcgacatc tcgaagcaat ggtcgaacta tgcggactcg
     5161 tcgcagttcc cggcctctgc catccacaag cttgacggca aggtctactc cgttcagggc
     5221 tacgtgaacc tgctcgggct ctggtacaac gccgacatcc tgaagaagat cggggtgcag
     5281 cctccgacga ccatgggcga gctgaactcc gacatggcca aggctgtcaa ggccgggttc
     5341 ggaggcataa cgctttcggg ccagccccag agccagggcg agtttcaggc gtacccgtgg
     5401 ttgaccgagg cgggcttcag ctacgcgaac ccgtcggtca agacgctcgc tgccggcctc
     5461 acgacggccc agcagtgggt caagaagggc tacctcaccg gcgaggccag cacgtgggat
     5521 cagaccgttc cgttccagaa gtgggcggcg ggcaagatcg cgttcgcgga gaacggcaac
     5581 tggcagatcg cgacggccaa gcagacggcg aagttcacgt acggcgtcgt gccgctgccg
     5641 ctcggggcga acggcaccaa ggtctatctc ggcggcgagg cagagggcat cggccggtac
     5701 tcgaagaacc cgtcgctggc gtggaagtac ctcgaggcca ccttcttcag caagaagggc
     5761 cagctgatcg cgctgaaaga cgagggcaac atcccctctc ggaaagacgc ggctgcggac
     5821 ccgcaggtca gcggcaacaa gc
//
LOCUS       DASTNL010000210         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_341070, whole genome shotgun sequence.
ACCESSION   DASTNL010000210 DASTNL010000000
VERSION     DASTNL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341070"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /gene="hisE"
                     /locus_tag="VFQ96_06680"
     CDS             complement(<1..161)
                     /gene="hisE"
                     /locus_tag="VFQ96_06680"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539721.1"
                     /note="catalyzes the formation of 1-(5-phosphoribosyl)-AMP
                     from 1-(5-phosphoribolsyl)-ATP in histidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphatase"
                     /protein_id="HEU0257507.1"
                     /translation="MKTFDTLFAELSDKARLRPEGSGTVAELDRGVHAIGKKIVEEAA
                     EVWLAAEHEG"
     gene            complement(228..893)
                     /gene="rpe"
                     /locus_tag="VFQ96_06685"
     CDS             complement(228..893)
                     /gene="rpe"
                     /locus_tag="VFQ96_06685"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU0257508.1"
                     /translation="MAAAHINPSVLAADFVHMAAELDRIRTADEVHVDVMDNHFVPNL
                     TFGPQMVGRIHDVSPVPLDVHLMIEDPDRWAPEYARLGAEGVTFHLEAAREPFTLAQT
                     LAGLGARPGVAVKPGTDPAPLLDHLDLFGMVLVMTVEPGFGGQRFMPETMPKLRLLRR
                     AADERGLDLRLEVDGGITAETIRTAAAAGADTFVAGSSVFGGASPIAAIAALRVAADE
                     TLG"
     gene            complement(963..2357)
                     /locus_tag="VFQ96_06690"
     CDS             complement(963..2357)
                     /locus_tag="VFQ96_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0257509.1"
                     /translation="MSGRRRSGHATPARRVAYDVLAAVEGSDAYANLLLPARLGRAGL
                     TGAEAGLATELCYGTLRMQGYYDAVLAAAGGRAVDSLDAPVRCVLRLGAHQLLGMRVA
                     GHAAVNESVSLAKEVGAAKASGLVNAVLRRVAARPAAGWRRSLLALVTGDERLALEYS
                     HPLWIVHALREALEADGQAPGALGRLLAADNTPAEVNLAALPGVALPKTLVPDRFSPV
                     GYRLPGGDPAPLLANGEGLLRVQDEGSQLVALTMARARPAQPSERWLDLCAGPGGKAA
                     LLAAEAAAAGAHLWANEVVPARRRLVERALAPWPDTVQVTEGDGRTLGHERPASFDRV
                     LVDAPCTGLGALRRRPEARWRKEPSDVARLQALQRELLASALGCVRPGGIVVYATCSP
                     HVAETRAIVDAAVGRSGVRLLAAGPILAGLVRAAAPVPADRTVAQLWPHDHGTDGMFV
                     AVLTREGDESGAAA"
     gene            complement(2354..3319)
                     /gene="fmt"
                     /locus_tag="VFQ96_06695"
     CDS             complement(2354..3319)
                     /gene="fmt"
                     /locus_tag="VFQ96_06695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0257510.1"
                     /translation="MTMARALKGLRIVFAGTPEVAVPALRRLAAGRHRVVGVLTRPDA
                     PLGRRRTPTASAVAREAALLGLPVVTANRPDETTARQVHAWAPDLGVIVAYGGLLRQP
                     LLDVPAHGWINLHFSALPRWRGAAPVQRAILAGERATALDVFRLVPALDAGEILASRS
                     QGLPADETAGQALERFAGQGAGLLEEVVDAIARGDAAGRPQEGDATYATKLTRDDGRL
                     DWARPAAEIYNRFRAVTPEPGAFTTLAGQTVKIHDMRRSGAHPGPAPDPAALAAGEAR
                     GIPNGVLVGTGTTPLLLVTLQPAGKRAMPAEDWWRGHGRAGLRLG"
     gene            complement(3316..>3615)
                     /locus_tag="VFQ96_06700"
     CDS             complement(3316..>3615)
                     /locus_tag="VFQ96_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257511.1"
                     /translation="REALREAELLDILGPVPVARPAAGTGGPARAAGDPAPDTAGSEG
                     LARLTVRFTYGQAAGVARVLRGLIVRAATAPRAAAGRGRRTPPTLKVRFDDPEAL"
BASE COUNT          463 a         1317 c         1386 g          449 t
ORIGIN      
        1 ccctcgtgct cggccgccag ccagacctcg gcggcctcct cgacgatctt cttgccgatc
       61 gcgtgcaccc cacggtcgag ctctgcgacc gtgccggaac cctccgggcg cagccttgcc
      121 ttgtcgctga gttccgcgaa tagggtgtcg aaggtcttca ctcacccaga ataccggtac
      181 ggcctgcgca ccgcgtcgcc cggcgccggc cgccggcacc gcccggctca gccgagcgtc
      241 tcgtcggccg cgacacgcaa cgccgctatc gccgctatcg gcgaggcccc gccgaacacg
      301 ctggagcccg cgacgaaggt gtcggcgccg gccgccgcgg cggtgcggat ggtctctgcg
      361 gtgatgccgc catccacctc gagcctgagg tcgaggcccc gctcgtcggc tgcccggcgc
      421 aacaggcgca gcttcggcat cgtctcgggc atgaagcgct ggccgccgaa acccggctcg
      481 acggtcatca cgaggaccat tccgaagagg tcgagatggt cgaggagggg ggccgggtcg
      541 gtgccgggct tcacggccac acccggccgg gctccgagcc ctgcgagggt ctgcgcaagg
      601 gtgaacggct ccctggcggc ctcgaggtgg aaggtgacgc cttcggcacc gaggcgcgcg
      661 tattcggggg cccaacggtc ggggtcctcg atcatcagat gcacgtcgag cggcacgggg
      721 ctgacgtcgt ggatccgccc gaccatctgg gggccgaagg tcaggttcgg cacgaagtgg
      781 ttgtccatca catcgacatg cacctcgtcg gcggtgcgga tgcgatcgag ctcggccgcc
      841 atgtgcacga aatcggcggc gagaacgctc gggttgatgt gcgctgcggc catgcctctc
      901 agcctacggc cgggcgcggc gcctccccgt gtccgccgcc cgccgacccc ggggcgcgcc
      961 cctcaggcgg cggcgcccga ctcgtcgccc tcccgggtca agacggccac gaacatgccg
     1021 tcggtgccgt ggtcgtgcgg ccacagttgc gccacggtcc ggtctgcggg cacgggcgct
     1081 gcggcgcgga cgaggccggc caggatcggc cctgcggcca agaggcgcac cccgctgcgg
     1141 ccgaccgccg cgtcgacgat cgcccgggtc tcggccacgt gcggagagca ggtggcatag
     1201 accacgatgc cgccgggccg cacgcagccc agggcggagg cgagcagttc gcgttgcagc
     1261 gcctgtagcc gggcgacgtc ggaaggctcc ttgcgccagc gggcctccgg ccgccgcctc
     1321 agtgcaccga ggcccgtgca gggcgcgtcg acgagcaccc ggtcgaagga ggccggccgc
     1381 tcgtgcccca gcgtgcgccc gtcgccctcg gtgacttgca ccgtgtcggg ccacggtgca
     1441 agcgcacgct ccacgaggcg ccggcgggcg ggcacgacct cgttcgccca caggtgggcc
     1501 cccgcggcgg cagcctcggc ggcgaggagc gcggctttgc cgcccgggcc ggcacagagg
     1561 tcgagccatc gctcgctggg ctgtgcgggc cgggctcgcg ccatcgtgag ggcgacaagc
     1621 tgcgaccctt cgtcttgcac ccgtaggagg ccttcgccgt tcgccaggag cggggccggg
     1681 tcgccgccag gcaaccggta gccgaccggc gagaatcggt cgggcacgag cgttttcggc
     1741 agggcgacgc cggggagggc ggcgaggttg acctcggccg gggtgttgtc tgcggccaag
     1801 agcctgccca gcgcccccgg cgcctggccg tcggcctcga gggcttctcg gagcgcgtgc
     1861 acgatccaca gcgggtggga gtattccagc gccaggcgct cgtcgccggt cacgagtgcc
     1921 aagaggctcc ggcgccaacc ggcggccggg cgcgccgcga cccgacgcag caccgcgttg
     1981 acgaggcccg aggccttggc ggcgccgacc tctttggcga ggctgacgga ctcgttgacc
     2041 gcggcatggc cggcgacgcg catgccgagc agctgatgtg cgccgaggcg cagcacgcaa
     2101 cgcacgggag cgtcgaggct gtcgaccgcg cggccgccgg ccgccgcgag cacggcgtcg
     2161 tagtagccct gcatgcgcag ggtgccgtaa cacaactctg tggccaggcc cgcctcggcg
     2221 cccgtgaggc cggcacggcc gagccgtgcg ggcaagagca ggttggcgta cgcgtcggag
     2281 ccttcgacgg cggccagcac gtcgtaggca acccgtcggg ccggcgtcgc gtgcccggaa
     2341 cggcgtcgcc cgctcatccg agccgcagcc cggcgcggcc gtggccgcgc caccagtctt
     2401 cggccggcat ggcgcgtttg ccggccggct ggagcgtcac gagaaggagt ggcgtcgtgc
     2461 cggtgccgac caacacaccg ttcgggatgc cacgcgcctc gcccgcggcg agggctgcgg
     2521 ggtcgggcgc cgggcccggg tgcgcgcccg accgccgcat gtcgtggatc ttcaccgtct
     2581 ggccggcgag ggtcgtgaag gcgccgggct cgggcgtcac ggcgcggaac ctgttgtaga
     2641 tctcggcggc cggccgcgcc caatccaacc ggccgtcgtc gcgcgtgagc ttcgtcgcgt
     2701 aggtcgcgtc gccctcttgc ggccggcccg cggcgtcgcc tcgcgcgatc gcgtcgacga
     2761 cctcctcgag aaggcccgcg ccttgcccgg cgaaacgctc gagcgcctgg cccgcggtct
     2821 catcggccgg caggccctgg ctgcgggagg cgagaatctc gccggcgtcg agggccggca
     2881 cgagacggaa gacgtcgagc gccgtcgccc tctcgccggc caggatcgcc cgttggacgg
     2941 gcgctgcacc ccgccaacgc ggcagggcgg agaagtgtag gttgatccag ccgtgggccg
     3001 gcacgtcgag aagcggctgc cgcaggaggc cgccgtacgc gacgatcacg ccgaggtcgg
     3061 gagcccaggc gtgcacctgg cgcgcggtcg tctcgtcggg tcggttcgcc gtcacgacgg
     3121 gcagcccgag gagcgccgcc tcgcgtgcga cggcggaggc cgtgggcgtg cgacgccgcc
     3181 cgagcggagc gtcggggcgg gtgagaacgc cgacgacacg gtggcggccg gccgcgaggc
     3241 ggcgaagggc cggcaccgcg acctcggggg tgcccgcgaa gacgatccgg agccccttca
     3301 gggcgcgagc catggtcata gcgcctccgg gtcgtcgaat ctcaccttga gtgtaggggg
     3361 cgtgcggcgg ccgcggcccg cggcggcacg cggcgcggtc gcagcccgca cgatgaggcc
     3421 gcgcagcacg cgggcgacgc ctgcggcctg gccgtaggtg aagcgcacgg tgagacgcgc
     3481 gagcccttcg ctccccgccg tgtcgggagc cggatcgccc gcggcgcggg ccggcccgcc
     3541 ggtcccggcg gcgggccgag cgacggggac agggccgagg atgtcgagca gctccgcctc
     3601 gcgcaacgcc tcccg
//
LOCUS       DASTNL010000211         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_341233, whole genome shotgun sequence.
ACCESSION   DASTNL010000211 DASTNL010000000
VERSION     DASTNL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341233"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VFQ96_06705"
     CDS             <1..1303
                     /locus_tag="VFQ96_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0257512.1"
                     /translation="SGGWVSAIGGWHQTQFAGTWLPTRAELDRVAPHNPVYVQSLYEV
                     GIANSAAIEAVGLQDAARRLPAGTVERDPSGEPTGVVRALPAFNLFLAAVGTPGPDEE
                     IAGTRAMARDYAALGLTGVLDPGGFGMSRERYAALHELHRTGGLNLRMRLFDSATTPG
                     DEVRQVEEWLQALPDEGGDDWLRSVGIGEVVHFGCHDFEGLDDFAIADEARDTLTSIS
                     RSVAKRGVPMHIHAVMDDSIGLILDCWEEVGRTTPITGLRFSFAHVDRISARNIARAK
                     ALGVGVIVDARLAFRSAASQAVWGTGSLHAAPPLGDIVASGLPLGVGSDATRASSHNP
                     WLSAWWLVAGRSLDGTSQRDPGHLLDRERTLVAQTIGNAWFSFEEDRRGALRPGWWSD
                     LAVLSDDYFAVPDDAIRDLRSELTLVGDRIAYSSGSLADAA"
     gene            1496..4168
                     /locus_tag="VFQ96_06710"
     CDS             1496..4168
                     /locus_tag="VFQ96_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0257513.1"
                     /translation="MLRPVDLLHHRTSLTDRWQHIVDAVADHVAFSSVDTSYTFAQAD
                     TASGHIRGHLALASGGDHETPIGLLAGHDPHALVGLLGIWRAERVAVVLDPHLPEERL
                     RNIAETAGLGIVIADEEHTGVATALVGEGGRVLRLEEILQTEPAARGQAGEPPVRHGT
                     DAAAIVFTSGSTGRPKGVIQTHGGLLNEAYAHGELWGITPEDRVALVFPYSFAAGLYV
                     LCIATLNGAAVHTFDPRDKGVHHLSAWLHDNEISTLHGTPYLIRSLVETLGPNETLDS
                     LRLVSTAGEAIYGTDVRALMPHLTPKASYANWLGSSEMGGFSTFAVHHGDVVPAGPLP
                     AGTPVANKEVTILREDGTEAGRGEIGTVVAISAYLSGGYWHDDEANAARFTPLADGRT
                     ACKQGDLGKIDDAGNLILAGRSDAAIKVRGYLVEPSEVEAALLRIDEVTETIVMGVHN
                     PPDPDHLVAYVVCDPQRRAPSVAALRRELRRSLPEYMVPPTIVQLTGLPRTERGKVDR
                     MGLPAPTPVTPSTPPSGGYELVLADIWAEALGLDSVGLDDDFMALGGDSLAVEEALAM
                     VRERLGVYLPSSQLLEAPTLREFHQRVQFSKRSGLPSHPDVVALRDAGEKTPIHCFAG
                     AGALALTFHPLSRHLPGRRVYGYQAHGLERRGLPDHTVAAAARRYLETLRVLQPKGPY
                     ILLGHSFGGLVALEVADTLARAGEEVQLVGLLDTYLPASITDSDFDYQRLPGGPHRNA
                     RWDLTRQAERWLHRLLPEGIPALADWGRHARSYVAGAIQFDGQDQFDAFFDLLKRVAR
                     RHEVHSYAGRTLLVIAENNPDGYEAWRPFLTGPREIVQIASEHTSLLREPYAAELADV
                     VRSRLGEAPDESLSGAESGNEPATDEALAAHQAS"
     gene            4422..>4883
                     /locus_tag="VFQ96_06715"
     CDS             4422..>4883
                     /locus_tag="VFQ96_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU0257514.1"
                     /translation="MMPYKFGIDEVNTKIQILREEFNQLHSYNPIEHVSSRLKTPESL
                     VEKVTRRGIEPSFEAMRETITDIAGVRVTCSFVSDVYRVFDLITGQNDLRLLVAKDYI
                     ATPKPNGYKSLHAIVEVPVFLSTGSVDVPVEVQFRTIAMDFWAALEHKIHYK"
BASE COUNT          769 a         1770 c         1632 g          712 t
ORIGIN      
        1 ctccggcggg tgggtgagcg cgatcggcgg ctggcaccag acacagttcg ccggtacctg
       61 gctgccgacc cgcgccgagc tcgaccgggt agcgccccac aacccggtct acgtgcagtc
      121 gctgtacgag gtcggtatcg ccaacagcgc cgcgatcgag gcggtcgggc tgcaggatgc
      181 ggccaggcgc ctgccggccg gcaccgtgga gcgcgacccg tcgggtgagc cgaccggggt
      241 cgtgcgcgca ctgcccgcgt tcaatctgtt tcttgcggcc gtcggcaccc cgggccccga
      301 cgaagagatc gccggcaccc gagcgatggc gcgcgactat gcggcgctcg ggctgacggg
      361 tgttctcgac ccgggcggtt tcggcatgag ccgggagcgc tacgccgcgc tgcacgagtt
      421 gcatcgcacg ggcggcctga acctgcgcat gcgactcttc gattccgcga cgacgccggg
      481 cgacgaggtc cggcaggtgg aggaatggct ccaggccctg cccgacgaag gcggcgacga
      541 ttggctgcgc tcggtcggca tcggcgaggt cgtgcacttc ggctgccacg acttcgaagg
      601 tctcgacgac ttcgccatcg ccgacgaggc ccgcgacacc ctgacgagca tcagccgcag
      661 cgtcgcgaaa cgcggcgtgc ccatgcatat ccacgccgtg atggacgaca gcatcggctt
      721 gattctcgac tgctgggaag aggtggggcg cacgacgccg atcaccggcc tgcgcttctc
      781 cttcgcccat gtcgaccgca tcagcgcccg gaacatcgcc cgagccaagg cgctcggcgt
      841 aggcgtcatc gttgacgcgc gtctcgcctt ccgctcggcc gcctcgcagg ccgtgtgggg
      901 cacagggtcc ctgcacgccg caccgccgct gggcgacatc gtcgcatccg gcctgccgct
      961 cggggtcggg tcggatgcga cccgggcctc ctcccacaac ccgtggctgt cggcatggtg
     1021 gctcgtcgcc gggcgctcgc tcgacggcac gtcacagcgc gaccccggcc acctgctcga
     1081 ccgggagcgc accctcgtcg cacagacgat cggcaacgct tggttctcgt tcgaagaaga
     1141 ccggcgcggc gccctgcggc ccggctggtg gtcggatctc gccgtgcttt ccgacgacta
     1201 cttcgccgtg cccgacgacg cgatccgcga tctccgctcc gagctgacac tcgtcggcga
     1261 ccgcatcgcc tactcgtcgg gctccctcgc cgacgcggcg tgacgagccg gtgcgaccac
     1321 accccttgcc gggtggcgcc ttcgcgagaa gactgaagca gtcatcgcac cagcctccgg
     1381 ctaagacgcg gccaggcgcg ccctctggtc ggagagttca cggaaggcaa tctgcctccc
     1441 ccgaccagtg tcggacgtcc tccctcgagc aagcccccgg cgaaaggtac agcgcatgct
     1501 ccgccccgtc gacctcctcc accatcgcac ctcactgacg gaccgctggc agcacatcgt
     1561 cgacgcagtc gccgaccacg tcgctttctc ctccgtcgac acctcgtaca ccttcgccca
     1621 ggcagacacc gcgtcggggc atatccgcgg gcacctcgcc ctcgcctccg gcggcgacca
     1681 cgagaccccg ataggcctgc tcgccggcca cgacccgcac gccctcgtcg ggcttctcgg
     1741 catctggcgc gccgaacgcg tcgctgtcgt cttagacccg cacctccccg aggaacggct
     1801 tcgcaacatc gccgaaaccg cgggtctagg catcgtgatc gccgacgagg agcacaccgg
     1861 tgtcgcgacg gcactcgtcg gagaaggcgg gcgggttctg cgcctcgaag agattctcca
     1921 gaccgagccc gcggcacgcg gccaggccgg cgagcctccc gtgcgccacg ggacggacgc
     1981 ggccgcgatc gtcttcacct ccggctcgac cggccggccg aagggcgtga tccagactca
     2041 cggcgggttg ctcaacgagg catacgcgca tggcgagcta tggggcatca cgccggaaga
     2101 ccgcgtcgcg ctggtctttc cctacagctt cgcggcgggg ctctacgtgc tgtgcattgc
     2161 gacgctgaac ggggccgccg tgcacacctt cgatccccgc gacaaaggcg tgcaccacct
     2221 gtcggcctgg cttcacgaca acgagatctc taccctgcac ggcacgccgt acctcatccg
     2281 ctcgctcgtg gaaaccctcg gcccgaacga aaccctcgat tcgttacgcc tcgtctccac
     2341 cgccggcgag gcgatctacg gcaccgacgt gcgggccttg atgccgcacc tcacaccgaa
     2401 ggcctcgtac gccaactggc tcggatcctc cgagatgggc ggcttcagca ccttcgcggt
     2461 gcaccacggt gacgtcgtgc cggcagggcc gttgccggcc ggcacccccg tcgccaacaa
     2521 agaggtcacg atcctgcggg aagacggtac ggaggccggg cggggcgaga tcggcaccgt
     2581 cgtcgcgatc tccgcgtacc tctcgggcgg ctactggcac gacgatgaag ccaacgcggc
     2641 acgtttcacc ccgctggccg acgggcggac ggcctgcaag caaggcgacc tcggcaagat
     2701 cgacgacgcc ggcaacctga tcctggcggg ccgctccgac gccgccatca aggtgcgcgg
     2761 ctacctggtc gagcccagcg aggtggaggc ggcgctgctg cggatcgacg aggtcacgga
     2821 aaccatcgtc atgggggtgc acaaccctcc cgacccagac cacctggtcg cctacgtcgt
     2881 gtgcgacccg cagaggcgcg cgccctccgt cgccgcgctc cgccgcgagc tgcgccgatc
     2941 cctgccggag tatatggtgc cgccgaccat cgtgcagttg accgggctgc cgaggacgga
     3001 gcgcggaaag gtggaccgca tggggctgcc cgccccgacg cccgtcaccc cttccacccc
     3061 gccgtcgggc gggtacgagc tggtgctggc cgatatctgg gcagaggcct tgggcctcga
     3121 ctccgtcggc ctcgacgacg atttcatggc gctgggcgga gactcgctgg ccgtcgaaga
     3181 ggcactcgcg atggtccggg aacggctcgg cgtatatctg ccgtcaagcc agctgctcga
     3241 agcgccaacg ctgcgggagt tccaccaacg cgtccagttc tcgaagagat cgggcctgcc
     3301 gagccacccc gacgtggtag cgctgcgaga cgccggcgag aagacgccca ttcactgttt
     3361 cgccggcgcg ggtgccctgg cactcacgtt ccatccgctc tcgcggcacc tgccgggccg
     3421 gcgagtctac ggctaccagg cgcacggcct ggagcggcga gggctgccgg accacacggt
     3481 ggcggcggcc gcccgccgct acctggagac ccttcgggtc ctgcagccga aggggccata
     3541 cattctcttg ggccactcgt tcggaggtct cgtcgcgttg gaagtggccg acacgctggc
     3601 ccgcgccgga gaagaggtgc aactcgtcgg gctgctcgac acctacctgc cggcgagcat
     3661 caccgacagc gacttcgact accaaaggct ccccggcggg ccgcaccgca acgcccgatg
     3721 ggacctgact cgccaggcgg agagatggct gcaccgcctg ctgccggagg gcatccctgc
     3781 gctcgccgat tgggggcgcc acgcgcgctc ctacgtggcc ggcgcgatac agttcgacgg
     3841 ccaggaccag ttcgacgcgt tcttcgacct gctgaagcgg gttgcgcggc ggcacgaggt
     3901 gcacagctac gcggggcgca cgctgctcgt catcgcggag aacaacccgg acggctacga
     3961 ggcgtggcgg cccttcctca ccggcccccg cgagatcgtg cagatcgcgt cggagcacac
     4021 gtcgctcttg cgggagccgt acgccgccga gctggccgac gtcgtgcgca gccgcctggg
     4081 cgaggcgccg gacgagagcc tctctggtgc ggagagcggg aacgagcccg ccaccgacga
     4141 ggcgctcgcc gcccatcagg cgtcgtgacc gggctgccgt cgggccccgt gcgtgggtgc
     4201 cgaccgccca ggccgcccag cgatagcgtg gaagggtgag cgatgtagcg cagcggcaag
     4261 ccgccgacga cgacggggcg gcacgcccgg ccggcaacga tccctggggc tcgcggccac
     4321 agagcccttc gttgcgagac cgtgcggcca gggcgttggg cgggctcgac gacgacggac
     4381 tgggggcggt cgtctcactg cgcgacgact tcgcacggtt catgatgccg tacaagttcg
     4441 gcatcgacga ggtgaacacg aagatccaga ttctgcgcga agagttcaac cagctgcaca
     4501 gttacaaccc gatcgagcac gtctcgagcc ggctgaagac tccggagagc ctcgtcgaga
     4561 aggtgacgag gcgcggcatc gagccgtcgt tcgaggcgat gcgggagacg atcaccgaca
     4621 tcgccggcgt gcgggtgacg tgcagcttcg tctccgacgt gtatcgcgtg ttcgacctca
     4681 tcaccggcca gaacgacctc cggctgctcg tcgcgaagga ctacatcgcc accccgaagc
     4741 cgaacggcta caagagcctg cacgccatcg tcgaggtgcc ggtattcctg tccaccggca
     4801 gcgtcgacgt accggtcgag gtgcagttcc gcaccatcgc catggacttc tgggccgccc
     4861 tcgagcacaa gatccactac aag
//
LOCUS       DASTNL010000212        25753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_712022, whole genome shotgun sequence.
ACCESSION   DASTNL010000212 DASTNL010000000
VERSION     DASTNL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 25753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25753
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712022"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1453)
                     /locus_tag="VFQ96_06720"
     CDS             complement(392..1453)
                     /locus_tag="VFQ96_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0257515.1"
                     /translation="MRESSVAKAGRAPDPEAPGEPEAPTQIPRASWRYIIVRSGQEFL
                     WDDCMQIAAALTYYAVLSLFPALLAVVAVLGLVGKAKTVTDAVLSAIGQLAPGPAVDL
                     LRGPVHELATSEAAGLALVVGVLGALWSASAYVRAFGRAVNRVYEVDEGRPFWRLVPT
                     TLAVTVLGVAIAAVMSATLALSGGVARAVGNAFGVAHAAEAVWSVAKWPVLVALAIVA
                     VSVLYYVTPNVRRPKFRWVSIGAAVALALAALATTGFAFYVANFAHYNKTYGAIGSVI
                     ILLIWVWIVNLALLFGAEFDSEVERGRQLQAGIRAERAIQLPPRDDRQSVRRAAREEK
                     AVARGRELRERLAAGRAAR"
     gene            complement(1541..2542)
                     /locus_tag="VFQ96_06725"
     CDS             complement(1541..2542)
                     /locus_tag="VFQ96_06725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0257516.1"
                     /translation="MTLPLNVLDLAPRPAGGTNADALAGSIKLAQRAEELGYQRFWVA
                     EHHGMPAIASSAPAVLIAAIAARTQRIRVGSGGVMLPNHAPLVIAEQFGTLRALYGDR
                     IDLGIGRAPGTDGLTAAALRRSAEKLGAADFPQQLVDLFGFFYGMPEDNPLHVITARP
                     GRGDAPEVWLLGSSGYSAQVAAALGLPFTFAHHFAGDHTEEALTLYRNAFEPSEMLAE
                     PHAMVAVNVISDDDPEVVRREALPGRITYLQMRRGQKPEPVALADALAYEFSPAESQF
                     LAAKEARQAVGTPPEVRAALRGLLASTEADELMIASNAASLPGRIRSLEVAAAVLAD"
     gene            complement(2580..3263)
                     /locus_tag="VFQ96_06730"
     CDS             complement(2580..3263)
                     /locus_tag="VFQ96_06730"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU0257517.1"
                     /translation="MAKLYFRYGAMNSGKSTAMLQAAHNYEERGHVVLLAKPAADTKG
                     DADIVSRLGVRRPVDFLLQPGADAYETFTRHRAAARRAGHDVSALLIDEAQFLTEAQV
                     DDLLRIAILEGVPVLAYGIRTDFQTVAFPGSRRLLEIAHSIEELKTICRCGKKAIFNA
                     RTIDGVFVFDGDQVAIDTSHTSDGAGPKVGYEALCASCYLEESHGVLNRRGPWSAGGQ
                     EREPDADFA"
     gene            complement(3423..4895)
                     /gene="mqo"
                     /locus_tag="VFQ96_06735"
     CDS             complement(3423..4895)
                     /gene="mqo"
                     /locus_tag="VFQ96_06735"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEU0257518.1"
                     /translation="MATSSYDVVLVGGGIMSATLGALIQRVQPDWSIRIFEVLGGVAR
                     ESSNPWNNAGTGHSGLCELNYMPDSDGPLDPSKPIHVNEQAQLSRQFWASLVRSGSLP
                     DPSVFLHSAPHMSFVRGKDNVDYLRRRYETLKDLPLFAGIEFADTPEKIAEWAPLLIE
                     GRAPRQYVAATRSPAGMDVNFGALTKALFEDQIAHGAVLSLNTRVSGIKRRKDGLWRV
                     KVRHVVGQTPYQVTARFVFVGAGGGALHLLQRTHIPEIKGYGGFPVSGKFFRTTDPAL
                     VAQHTTKVYGKAAVGAPPMSVPHLDRRVVDGQPSLLFGPYAGFSPKFLKNSTWLDLPR
                     SLRFGNIMPMLAVARDNFALIKYLVGELLASRTKQLRALQQFVPNAKSEDWELITAGQ
                     RVQVIKKDKERGGKLQFGTEVITAADGSIAGLLGASPGASTAVPIMLNLLKTCFPSRY
                     PDWEPRLKELVPTLGEELNRQPALAEATMAATAETLQIPA"
     gene            complement(5005..6096)
                     /locus_tag="VFQ96_06740"
     CDS             complement(5005..6096)
                     /locus_tag="VFQ96_06740"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU0257519.1"
                     /translation="MNAYDDGYSVAVVGATGQVGGVMRRLLAERHFPVKAIRFFASAR
                     SAGTVLSFEGHDVVVEDAATADPSGIDIALFSAGGAGSRALAPKFAAAGAIVIDNSSA
                     WRMDPHVPLVVSEVNPAATAEAFGPDGRGIIANPNCTTMAAMPVLKVLDKEAGLTRLI
                     VSTYQAVSGSGLAGARELARQAQAATANENLLGLVHDGRAVTMPEPVKYVRPIAFDVV
                     PFAGNLVDDGLNETDEEKKLRNESRKILGLPDLLVSGTCVRVPVFTGHSLSINAEFAR
                     PLSPERATELLADAPGVRLTDVPTPLEAAGNDPSYVGRIRADEGAPEGRGLALFVAND
                     NLRKGAALNAVQIAELVTARLAAGRVAAA"
     gene            complement(6173..7477)
                     /locus_tag="VFQ96_06745"
     CDS             complement(6173..7477)
                     /locus_tag="VFQ96_06745"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU0257520.1"
                     /translation="MALIVQKYGGSSVADAESIKRVAKRIVETHKAGNEVVVAVSAMG
                     DTTDDLIDLAHRVVPIPDPREFDMLLTAGERISMALLAMAIGSLGHDAVSFTGSQAGM
                     ITTAQHGAARIVDVTPGRVKDALSEGKIAIVAGFQGFNRDTHDITTLGRGGSDTTAVA
                     LAAALDASVCEIYTDVDGVFTADPRVVPLAKKLSRITSEEMLELAGSGAKVLNIRAVE
                     YARRNGVTLRVRSSFSNDEGTLVVSHPSEEYAAYIAAKHKKGKSMEEPIIAGVATDLT
                     QAKITVIGVPDVPGAAARIFKVVTKANANVDIIVQNVSAATTGTTDISFTLPKADGQR
                     VVTALNAERHELGFGPLQYDDQIGKLALVGAGMRSNIGVSLQLFEALADAGINIEMIS
                     TSEIRVSVITRADMVNEAARVVHRAFGLDGETQAVVYAGTGR"
     gene            complement(7696..8292)
                     /gene="recR"
                     /locus_tag="VFQ96_06750"
     CDS             complement(7696..8292)
                     /gene="recR"
                     /locus_tag="VFQ96_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0257521.1"
                     /translation="MYEGIVQDLIDELGRLPGIGPKSAQRIAFHIVQTEGFDVSRLAH
                     ILLDLRDKVHFCQECGNISEQERCAICRDARRDRSRICVVEEAKDVAAIERTHEFHGL
                     YHVLGGAISPIDGIGPEELRLRELMKRLADGSVTEVIIATDPNLEGEATATYISRMLA
                     PLGLTVTRLASGLPVGGDLEYADEVTLGRAFEGRRVVG"
     gene            complement(8296..10548)
                     /locus_tag="VFQ96_06755"
     CDS             complement(8296..10548)
                     /locus_tag="VFQ96_06755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HEU0257522.1"
                     /translation="MVTALYRRYRPETFAEMIGQAQVTEPLMTALRTNRVGQAYLFSG
                     PRGCGKTTSARILARCLNCAKGPTDTPCGVCPSCVELGRGGHGSLDVVEIDAASHNGV
                     DDAREIRERAIFAPSRDRYKIFILDEAHMVTPQGFNALLKIVEEPPEHVVFLFATTEP
                     DKVIGTIRSRTHHYPFRLVPSAPMIEYIGTICADEGVTVAPGVLPLVARAGGGSPRDT
                     LSLLDQLIAGSEGDTVEYERAVALLGYTPAALLDEVVSALARRDAASAFEVVDRVIQT
                     GQDPRRFVEDLLERLRDLIVVTATAAEKAEGVLHGVPRDALERIAQEASRFGPAELSR
                     SADLVHAALTAMTGATAPRMHLELLMARLLIAPAGAAARDVVARLERLERRLDIGGPA
                     AEGRMAPPTASSTPPAPVTGGGGTSARPDADEGAAVTDEDRTHAAGAERGRSAGAGAG
                     AEPGAAAPPRDAPATRDPATVTAQQVRDAWPQVLAVVQEARRSAWIVLQTAQVQELRS
                     GPVLVLSFPSRRDVEALKDAGVAGHGVGDYLKDALDAVLGIRPGLVAKAAAPAASESA
                     DTAVVPGTVTRDRQRPSVRTATGGTVADPGATGPGVTGETAAGEAVTSWDVIAIPHPE
                     DSAERAAGPDAGEGESPEDDLVEEEPPEDAAFDETPAKAPVGDRAPGRHGQQEEASAP
                     GAAGASAGTSPAEGALQNGSRAHEAAAAPARGGVAFRRPDERQRYGEAVVREVLGARF
                     LEEQNLDGGD"
     gene            complement(10680..11885)
                     /locus_tag="VFQ96_06760"
     CDS             complement(10680..11885)
                     /locus_tag="VFQ96_06760"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU0257523.1"
                     /translation="MKPVLVINSGSSSFKYQLLDMDREETLARGLVERIGEDGGHASH
                     TREGAGETAETFERSLPIPDHTAGFRVMVDAFAAHGPSLGEHPPVAVGHRVVHGGERF
                     VEPTLVTDEVEQGIEDLADLAPLHNPANVQGIRAARSLFPGLPHVAVFDTAFHQSLPP
                     AAYHYAIDPDLAGRLRIRRYGFHGTSHKYVSEAAARFLRRPAAEVKQLVLHLGNGASV
                     TAVDGGLSVETSMGMTPLEGLVMGTRSGDIDPAVLLYLQRHGGYDVDGLDDLLNKHSG
                     LLGMSGHRDMRDVQAAAAAGDKAATDTLAVYLHRLKSYVGAYYAQLGHLDVISFTAGI
                     GENNAAVRAGSLAGLDELGIVVDPARNEAPGRGVRRISADDSRVAVLVVPTDEELEIA
                     RQTLRLAAA"
     gene            complement(11882..14023)
                     /gene="pta"
                     /locus_tag="VFQ96_06765"
     CDS             complement(11882..14023)
                     /gene="pta"
                     /locus_tag="VFQ96_06765"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU0257524.1"
                     /translation="MSKSIYITSAEGHTGKSTIALGMLDALAGTVAKVGVFRPVARSS
                     EEPDYVLRMLLEHQGLAGDYDRCVGTTYEAVHADPDDALAVIVARYKAMEADYDAVLI
                     VGSDYTDVGTPTELRFNARVAANLGAPVLLTLGGRVTAATRDHLGSPPSRTPGEIRQV
                     ADLTLAELHDEHAEVIAVIVNRADPDRLAEIIAETGAVTKARLGRAVVWALPEDPVLV
                     APTMRSIRQAIDGTLVHGDAELLGREALAVVIAAMSLENVLPRLIEGAVVIVPADRTE
                     VLLAVLMANASGTFPSVAGIVLNGGFNLPDVIVRLMDGVHSTLPIISTRLTSYDAAVR
                     ITDTRGRLAADSQRKHDSALALFRQHVDADRLLDLLNVTRQAVVTPLMFEYDLIARAR
                     AHRRHIVLPEGDDDRILRAAARLLAQGVAELTILGEIFEVRSRAIELGLDISAAHVLS
                     PFDHVLSLKFADEYARLRASKGTTRDLAADTITDPSYFGTMMVHMGLADGMVSGARHT
                     TAHTIRPAFEIVKTRPGVSVASSVFLMALADRVLVYGDCAVIPDPSAEQLADIALSAA
                     ETAAQFGIDPRVAMLSYSTGESGHGADVDKVRQATALVRQRRPELPVAGPIQYDAAAD
                     AAVGAAKMPGSDVAGRATVFIFPDLNTGNNTYKAVQRSAGAVAIGPVLQGLRKPINDL
                     SRGASVEDIVNTVAITAIQAAAQPAVKGMHR"
     gene            complement(14103..14199)
                     /gene="ffs"
                     /locus_tag="VFQ96_06770"
     ncRNA           complement(14103..14199)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFQ96_06770"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            14325..15617
                     /locus_tag="VFQ96_06775"
     CDS             14325..15617
                     /locus_tag="VFQ96_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU0257525.1"
                     /translation="MGHLPPLVEPGPELSLERLIRYSRQLALPGFDETAQRRLAAARI
                     LVIGAGGLGSASIPYLASSGVGTIGVVDDDVVELSNLHRQVMHTLAMLGRPKVESAAL
                     AALAIDPEIRVETHPVRVDATSLAAILGDYDLVLDGSDNFETRYLCADACAAAGKPLV
                     WGAILRFAGQVSVSWAGAGPTYRDLYPVQPAAGEVPNCAQAGVVPGVCAVIGSLMATE
                     ALKIVTGLGEPLVGRVTTYDALTGRFREIEYAAADDEPTVPGTGGRDAEDARPARAAA
                     AGTVAVGAAPAPAVPATPVGGATRPRGRASHPGAAAATPGRQAASFNPARAQISVRQM
                     ADWRQQGTTFQLVDVREPYEVRIAAIPGSEAIPLRSLGDELGRLRHDVPLVVHCHVGD
                     RSFQAVRYLHAHGYPNAVNLVGGIDAYARQVDTTMARY"
     gene            15623..16060
                     /locus_tag="VFQ96_06780"
     CDS             15623..16060
                     /locus_tag="VFQ96_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU0257526.1"
                     /translation="MESVMGVQSLVRLSRIVSEPIDEDAVRDAVAADENGATCLFLGV
                     VRNHDRGRNVVRLDYQAHPDAEVVLAALCAEVAQATGVAVAAEHRTGTLVVGDTALCA
                     AAGSGHRAEAFDACRILVERIKHEVPIWKRQHGADGTTEWVGL"
     gene            complement(16064..16597)
                     /locus_tag="VFQ96_06785"
     CDS             complement(16064..16597)
                     /locus_tag="VFQ96_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0257527.1"
                     /translation="MGGQAADRFLIVGVVPGQSEKVPLAGARLANLFDAELVCAHVDA
                     MRYVVQEGLDGSVVSSSVDPDVVEEGDEVFDEGLRARLAATFDGGPVRWSTRLLAGEP
                     AVALSHLAERLDAEAIIVGTRRAGFRSGMQEFFGGSVAVRLAHRQGRPVIVVPVGGEP
                     RTATGSILLPWGPADQL"
     gene            complement(16646..16888)
                     /locus_tag="VFQ96_06790"
     CDS             complement(16646..16888)
                     /locus_tag="VFQ96_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0257528.1"
                     /translation="MDVLVRYFAAAADAAGREEESLHFAPNATLGDLRSLLEKRYTGM
                     TDVLRNGSFLIDKVVRRSLDMPLAPRVDVLPPFAGG"
     gene            complement(16948..17361)
                     /locus_tag="VFQ96_06795"
     CDS             complement(16948..17361)
                     /locus_tag="VFQ96_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0257529.1"
                     /translation="MTQYRISEAAALLGVSDDTVRRWSASGDLPVTTDASGRQAVEGT
                     DLAAYAERIAAPAVDPLEGVTSARNRFVGLVTKIVTGPVVCQVELQCGPHRVVSVITT
                     QSCHDLGLEVGSVAVAAVKSTAVVVQVPEGVRPVR"
     gene            17443..18687
                     /gene="glp"
                     /locus_tag="VFQ96_06800"
     CDS             17443..18687
                     /gene="glp"
                     /locus_tag="VFQ96_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0257530.1"
                     /translation="MADIPASVSVREHLERVLTSIEPLLQTDAPLSRARGRVLAEDVR
                     ALVAIPSFDNSAMDGYAVHRDDVAEASTLRPVTLRVVADLPAGTAEDPAVGPGEAARI
                     MTGAPLPSDADAVIPVEDTDRGLPEVVVTAPATTSGYVREAGADTRIGEVVLTAGRIL
                     DSQDIATAAATGSRTLPVFPAPRVAILSTGSELLTPGEPLTRGQIYDANSYLLEAQVA
                     EAGGVPVRIGAIPDNLDRLGGALDEIVPGVDAIITSGGVSVGAYDVVKALLSGHGNVW
                     FGKVRMQPGGPQGFGHWSDGTPVFTLPGNPVSSFVSFEVYVRPALRKMQGLTPWERPV
                     VRAVAAQDWTGPAGRAQYMPVALVRGEAEEVPLHVRPSARGGSASHLVASLAAAEGIA
                     IVPEAVTLVEAGTNVDVMLVRN"
     gene            18687..19169
                     /gene="moaC"
                     /locus_tag="VFQ96_06805"
     CDS             18687..19169
                     /gene="moaC"
                     /locus_tag="VFQ96_06805"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU0257531.1"
                     /translation="MAAPKFTHVDARGEARMVDVTAKTPTVRAATASGFVWCAPIVMA
                     ALRDGETPKGDVLAVARIAGIQAAKRTAELLPLAHVIGVHGVVVDLAVEEEGVRITAT
                     VRTADRTGVEMEAFTAVSVAALAIVDMVKGIDRTSEIGQIHLEAKEGGRSGTWRRSTQ
                     "
     gene            complement(19181..19750)
                     /locus_tag="VFQ96_06810"
     CDS             complement(19181..19750)
                     /locus_tag="VFQ96_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU0257532.1"
                     /translation="MTQVTEEQGDTHTHRDGHHRAEKISAAVITVSDRASEGLRDDLS
                     GPRAVSILAEAGYSADTMVVPDGADSVTGALRAALADGARLIVTTGGTGVGPRDRTPE
                     GTREVVAYELPGIAEAMRRTGAVMHPTALLSRGIAGVTDERTRALIVNLPGSPDAVAE
                     GLQVVLPLVAHVIDQLDGGDHDAHGHHGH"
     gene            19976..20063
                     /locus_tag="VFQ96_06815"
     tRNA            19976..20063
                     /locus_tag="VFQ96_06815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20010..20012,aa:Ser,seq:gga)
     gene            complement(20140..21414)
                     /locus_tag="VFQ96_06820"
     CDS             complement(20140..21414)
                     /locus_tag="VFQ96_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0257533.1"
                     /translation="MAQTARPPDGTVDGPEEARRQRRSRSRHAGSLADYQTKKGKRWK
                     FQIYVLKDPESPELGETRVSRGGFADLEEAQAALAEALKKRARNEKFQGKVPTIATYA
                     TEWTKGLRLESSTVQGYEKIIRNHIVPQLGAIRLDKLTATRIGRHYRELEKSGRRDKR
                     GFGQPLSVNTVNKVHVVLGAILDAALDDGLIAVNPARKKRTVNAPTGSQIRAQRPEIA
                     TWTGAQLHSFLTWNRDVLGDALFPLWRTVAYTGMRRSEALALRWGDVNFGTSRLSIRR
                     AADVTTRNKTKTTKTGAARVLDLDADTLAVLRGYRAKRGAVSLDLARADAYVFGNDAG
                     AIRSPNEVGRRWTYRVKRAQEKVDGLPRITLKGLRHTHATLLLELGEHPKVVQERLGH
                     STITTTMNIYSHVTPTMQKAAVNRFAALLDEA"
     gene            complement(21450..22010)
                     /locus_tag="VFQ96_06825"
     CDS             complement(21450..22010)
                     /locus_tag="VFQ96_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0257534.1"
                     /translation="MGTERVRGNPAGVTQEHVAANLRAARQAIGMDVRTAARLMTEAG
                     RKLAASSISKIENGDRRVDVDDLTALAYILRTTPAALLTPPSEAVTLTGVPESFLAEE
                     IQAWIAGAVKLTTEDLARYWKEQRFQAINSTRWAEEMLAKYDRGQVGVTPREVYQERH
                     DRQTEREAHATGRLLQLDPNASLFNE"
     gene            22101..22343
                     /locus_tag="VFQ96_06830"
     CDS             22101..22343
                     /locus_tag="VFQ96_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0257535.1"
                     /translation="MRTVTVTLDELRVSRTAVITRAEAAAALGVDPRTITASIENGSI
                     PSVRLGRRVVIPREKFLRLFDAEISPRSGTDGVSSM"
     gene            22434..22730
                     /locus_tag="VFQ96_06835"
     CDS             22434..22730
                     /locus_tag="VFQ96_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257536.1"
                     /translation="MSETTVTRRAPAPYTRHNFDHVSDVMLYDIARHTASVLSSVLLN
                     LADGATDDAERGHWAARRRLVKQQMRALDVHDRAGLIAQDEVWRLEKLALAGNA"
     gene            22769..23770
                     /locus_tag="VFQ96_06840"
     CDS             22769..23770
                     /locus_tag="VFQ96_06840"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU0257537.1"
                     /translation="MFATTVVDDLFAGHTPGRGDPLLVLLGGQPAAGKTQAQAAILAE
                     CAADDLVEITGDDLREYHPDYARLAEQSPFAMPGVTAPVSGGLIGLALDHALTQRHSV
                     LLEGTFRDKEMVIATATRFADAGYRVMIVAVATPAPVSRLSTEMRSLDAGYPEVGRWT
                     PPDAHESALAGSAMVVSALEALPFVGRVQVFSREALLFDNSRTDTGEWAQPARAARIL
                     RQEQHRQLSVREAYFWLQDYAAVFATARARPGYFASETAPTYLRLQNDAQNMIHILSK
                     KPGAPLSQLSREQRVRAAILERVLPPESLPEPHKSGMRSQRSQPHPSSRPDPPVPRL"
     gene            complement(23983..>25753)
                     /locus_tag="VFQ96_06845"
     CDS             complement(23983..>25753)
                     /locus_tag="VFQ96_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0257538.1"
                     /translation="GERVIVDEAGMLDQDTAIALFTVCDTAAATVALVGDRAQLPAVG
                     RGGVLDMAAHIRGRTFDMAEVHRFADRAYAAVTMRMRDGTNPGDVFDQLNETGLIRLH
                     DSDEDARDAIAAEWREGEAITAATNDDARTLNDRIREVRVVRGLVDDTRVVFGNDGLS
                     IGAGDLIQTRHNNSELGVANRQTWTVQHVEADGSLRVREARAGRKRQRTVHLPAEYVT
                     EHTHLSYATTAYGIQGATVTGAHTLLTDTTTAAGVYVGMTRGRASNVLHIAAEDIADA
                     RAQFIDAMGRDRADRGLDVASAHAREDVAGLIENGPVTFINNQIETLLRRAEVAEARA
                     ALWQQADTALTELTAQETQKRAQAASRGRQTRHAVERVRAEIVVPILADAKAAVAQWR
                     DANAHEQATLQKIADASWLRKRRARTEHENARSHVRDVRERLTRAWGGVPKPTERPTA
                     WVERVTTPVINADPRLAEAEATHQSARNALYNRPERVRAARLAVYVRVFGAEKVMRDQ
                     FAFLKANPAQRCADLSQTAGRTRKEADLLRTLAPAEALERIRRAHAAEEEREARRTAR
                     QQTTREEFQRQRHTVRGRPTRGL"
BASE COUNT         3836 a         8809 c         8956 g         4152 t
ORIGIN      
        1 actatctcgt cttgatcatt tatactcgtt ggattgcgtc aggccctgga gggccgggct
       61 ggcggtcgca atgccaggtg aggccacagc caacgccgtc gacagaacgc ccacagcgaa
      121 gacagcagcc agcgccgatc cgggcagcct catccgccgt atcgttgaac gcttgcccgt
      181 caccccggta ccctcccgaa actcgcctcg ccaaccttgc cgctatgaga atgctattgg
      241 ctgtctggta accacctgag tccgggccgc ccccgttatt gtcgggggtg tggcgcctgc
      301 ccactctcgt accagtggcc gcgagcagcc tgcatccaac cggataattc ccggactaga
      361 gcgttagatc ctagtcgccc ggcggagtcg cctagcgggc ggcgcggccg gcggcgaggc
      421 gttcccgcag ctcacggcca cgggcgacgg ccttctcttc acgggcagcg cgccgcacgc
      481 tctggcgatc gtctcgagga ggcagctgga tcgcacgctc ggcccggatg ccggcctgaa
      541 gctgccggcc gcgttccacc tccgagtcga actccgcacc gaagagcagc gcgaggttca
      601 cgatccacac ccagatgagc aggatgatga cgctgccgat cgcgccgtac gtcttgttgt
      661 aatgcgcgaa gttggctacg tagaaggcga aacccgtcgt ggcgagcgcg gcgagggcca
      721 aggccacggc tgccccgatg ctcacccagc ggaacttcgg ccgacgtacg ttcggcgtga
      781 cgtagtacaa caccgagacc gcgacgatgg ccaaggccac gagcaccggc catttcgcga
      841 cgctccacac ggcctccgcc gcatgcgcca ccccaaatgc gtttcccacg gcacgggcca
      901 cgccgccgga gagcgcgagg gtcgcgctca tcaccgcggc gatcgcgaca cccagtacgg
      961 tgacggcgag ggtcgtgggc acgagccgcc agaacggcct gccctcgtcg acctcgtaga
     1021 cgcggttcac ggcccggccg aacgcccgca cgtaggcgga ggccgaccac agcgcgccga
     1081 ggacacccac gaccaacgcg agccccgccg cctcagacgt cgccagttcg tgcaccggcc
     1141 cccgcagcag gtcgacggcg gggccgggcg ccagctggcc gattgccgac agcaccgcat
     1201 ctgtcaccgt cttcgccttg ccgacgaggc cgagaaccgc gacgaccgcc aagagcgccg
     1261 gaaaaaggga gagcaccgcg taataggtca gcgcggccgc gatctgcatg cagtcatccc
     1321 acagaaactc ttggcccgac cgcacgatga tgtaccgcca cgacgctcgc ggtatctgtg
     1381 tcggcgcctc gggctccccc ggcgcctcag ggtcgggcgc tcggcccgcc ttcgccacgc
     1441 tcgattccct catgaggccg ccctctcgcc gtgtgctcac acggtatgtc ggcgcgcggg
     1501 ccccatcaag gggtgcgccg ctgctcggag gccgggtcac tcagtcggcg agcacggccg
     1561 ctgccacctc gagggagcgg atgcggccgg gaaggctcgc cgcgttcgac gcgatcatca
     1621 actcgtcggc ctccgtcgag gcgagaagac cccgcagggc ggcacgcacc tcgggcgggg
     1681 tgcccaccgc ttgcctggcc tctttggcgg cgaggaactg gctctcggcc ggcgagaatt
     1741 cgtaggcgag cgcgtcggcc aaggcgaccg gctcgggctt ctggccgcgc cgcatctgca
     1801 ggtaggtgat tcggcccggc agcgcctcac gacgcacgac ctccgggtcg tcgtcggaga
     1861 tgacgttcac cgcgaccatc gcgtgcggct cggcgagcat ctcggagggc tcgaacgcgt
     1921 tccggtagag ggtgagcgcc tcttccgtgt ggtcgccggc gaagtgatgc gcgaaggtga
     1981 aaggcagacc gagcgcggcc gcgacctggg cgctgtagcc gctcgagccg agcagccaga
     2041 cctcgggcgc atccccccgg cccggccggg cggtgatgac gtgcagcggg ttgtcctccg
     2101 gcatgccgta gaagaagccg aacaggtcga cgagctgctg cggaaagtcg gctgcaccca
     2161 gcttctcggc gctgcggcgc aacgcggccg ccgtgagccc gtcggtgccc ggcgcccggc
     2221 cgatgccgag gtcgatccgg tctccgtaga gggcccgcag tgtgccgaac tgttccgcga
     2281 tgacgagcgg ggcatggttg ggcagcatca cgccgccgct gccgacccgg atccgctggg
     2341 tacgtgccgc gatcgccgcg atcaagacgg cgggggcgct ggaggcgatg gccggcatgc
     2401 cgtgatgctc cgccacccag aaacgctggt agccgagctc ttccgctcgc tgggcgagct
     2461 tgatactacc cgccagggcg tcggcgttcg tgccgcccgc cgggcgcggc gccaggtcta
     2521 acacgttcaa cggcagggtc atgcgcacac ggctttctct cgcacaggtt ccgggctcct
     2581 cacgcgaagt cggcgtccgg ctcgcgctct tggccgccgg ccgaccacgg cccgcgcctg
     2641 ttcaagaccc cgtggctctc ttcgagatag cacgatgcac acagcgcctc gtagccgacc
     2701 ttcggccccg ccccgtccga cgtgtgggag gtgtcgatcg cgacctggtc gccgtcgaag
     2761 acgaagacgc cgtcgatcgt gcgagcgttg aagatcgcct tcttgccgca gcggcagatc
     2821 gtcttcagct cctcgatgct gtgcgcgatc tcgagcagcc gccggctgcc gggaaaggcg
     2881 acggtctgaa aatcggtgcg gatgccgtag gcgagcaccg gcacgccttc gaggatggcg
     2941 atgcgcaaga ggtcgtcgac ctgggcctcg gtgaggaatt gcgcctcgtc gatgagcagc
     3001 gcgctcacgt cgtggccggc ccggcgcgcc gccgcacgat gccgggtgaa cgtctcgtag
     3061 gcgtcggcgc ccggctgcaa caggaagtcg accgggcgac gcacgccgag gcgcgagacg
     3121 atgtcggcgt cgcccttcgt gtctgccgcg ggcttggcca agagcacgac atggccgcgc
     3181 tcctcatagt tgtgggccgc ctgtaacatc gccgtgcttt tgccgctgtt catagcgccg
     3241 taacggaaat acagtttggc cacgagacga tcttacgttc cgcctgcgcg cgccggccgg
     3301 cccgcgcaca ggccgccacg agggctctgg gaagcttgcg agggtgtgag gctgccgggg
     3361 tgaggatgca caggagagcc cgcccggcag gctgcgcctg ccgggcgtgt gtcggatctc
     3421 gttcaggcgg gaatctgcag ggtctcggcc gtggcggcca tcgtggcctc cgcgagcgcc
     3481 ggctgccggt tcagctcttc gccgagcgtc ggcacaagct ccttcagccg gggctcccag
     3541 tcggggtacc tcgacgggaa gcaggtcttc aagaggttca gcatgatcgg cacggccgtc
     3601 gacgcccccg gcgaggcgcc gagcaggccc gcgatggagc cgtctgccgc cgtgatcacc
     3661 tcggtgccga actgcagctt gccgccgcgc tccttgtctt tctttatcac ctgcacgcgc
     3721 tggccggcgg tgatcagctc ccaatcctcg ctcttcgcgt tcggcacaaa ctgctgcaag
     3781 gcgcgcagct gcttcgtgcg tgaggcgagc aactcgccga caaggtactt gatgagggcg
     3841 aagttgtcgc gcgcgacggc gagcatcggc atgatgttgc cgaaccgcaa cgagcgaggc
     3901 aggtcgagcc aggtgctgtt cttcaagaac ttggggctga agcccgcgta cgggccgaag
     3961 agcagggagg gctgcccgtc gaccacgcgg cggtcgaggt gcggcaccga catcggcggg
     4021 gcgccgacgg cggccttgcc gtaaaccttc gtcgtgtgct gggcgacgag ggccgggtcg
     4081 gtggtgcgga agaacttacc gctgacgggg aagccgccat accccttgat ctcggggatg
     4141 tgtgtgcgtt gcaagaggtg cagcgcgccg ccccccgcgc cgacgaacac gaaccgcgcc
     4201 gtcacctgat acggggtctg cccgacgacg tgacgaacct tcacccgcca caggccgtct
     4261 ttgcggcgct tgataccgct cacccgggtg ttgaggctga gcaccgcacc gtgcgcgatc
     4321 tggtcttcga acagggcctt cgtcaaggca ccgaagttga catccatgcc ggccggcgac
     4381 cgcgtggcgg cgacgtactg ccggggtgcc cggccttcga tgagcagcgg cgcccattcg
     4441 gcgatcttct ccggggtgtc ggcgaactct attccggcga agagcggcag gtctttgagg
     4501 gtctcgtaac gccggcgcag atagtcgacg ttgtccttgc cgcgcacgaa cgacatgtgg
     4561 ggtgccgaat ggaggaacac cgacgggtcg ggcaggctgc cggagcgcac caggctcgcc
     4621 cagaactggc ggctcagctg cgcctgctcg ttgacgtgga tcggtttcga cgggtcgagc
     4681 ggcccgtcgc tgtcgggcat gtagttcagt tcgcacagcc cggaatgccc ggtgccggcg
     4741 ttgttccacg ggttggaact ctcgcgcgcc acgcccccca gcacctcgaa aatgcgaatg
     4801 ctccagtcgg gttgcacacg ctggatcaac gcaccgagag tcgcgctcat gatcccgcca
     4861 ccgaccaaaa cgacgtcgta ggaggaagtt gccacgtttc gagtctactg gcgcgggtac
     4921 tatgtctgcg ccgagcgtgc accgaccgcg gcaggcccat cgctcacggt cgaaggcggc
     4981 ccggccgcgc cttagcctta gccgtcaggc ggccgcgacc cgcccggccg cgaggcgcgc
     5041 cgtgacgagt tcggcgatct gcaccgcgtt caaggcggcg cccttgcgaa ggttgtcgtt
     5101 ggccacgaac agggcgaggc cccggccctc gggcgccccc tcgtcggcgc ggatacggcc
     5161 cacgtagctg gggtcgttgc cggcggcctc cagcggggtc ggcacgtcgg tgagacgcac
     5221 gcccggcgca tccgccagaa gctccgtggc ccgttccggg gagagagggc gggcgaactc
     5281 agcgttgatc gacagggaat gcccggtgaa gaccggcaca cgcacgcagg ttccactgac
     5341 gagaaggtcc ggcaggccga gaatcttgcg gctctcgtta cgaagcttct tctcctcgtc
     5401 ggtctcgttc aagccgtcgt cgacgaggtt gccggcgaac ggcacgacgt cgaaggcgat
     5461 cggtcgcacg tatttgaccg gttcgggcat ggtgacggcg cggccgtcgt gcacgagccc
     5521 cagcaggttc tcgttcgctg tggcggcctg ggcctgccgg gcgagctccc gggcgcccgc
     5581 caggcccgag cccgagaccg cctggtaggt ggagacgatg aggcgggtga gccccgcctc
     5641 tttgtcgagc accttcaaga cgggcatcgc cgccatggtc gtgcagttcg ggttcgcgat
     5701 gatgccccgg ccgtcggggc cgaacgcttc ggccgtcgcg gccgggttca cctcgctgac
     5761 gacgagcggc acgtgcgggt ccatacgcca cgcactggaa ttgtcgatca cgatggcgcc
     5821 tgccgcggcg aacttcggcg cgagcgcacg gctgccggcg ccgccggcgg agaagagagc
     5881 gatgtcgata cccgacgggt cggccgtggc cgcgtcttcg acgacgacgt cgtgcccttc
     5941 gaacgacagc accgtgcccg ccgaccgggc ggatgcgaag aagcggatgg ccttcaccgg
     6001 aaagtgccgc tcggccagaa gacgccgcat gacgccgccg acctgcccgg tggcaccgac
     6061 gaccgcgacg ctgtacccgt cgtcgtaagc gttcattcct cttccttccc tcgcctaagc
     6121 cctcgcgccg gcccgcccgg gattcctgcc gggcacgtct cgcggagcct cgtcagcggc
     6181 cggtccccgc atagacgacg gcctgcgtct cgccgtcgag gccgaaggcg cggtgtacga
     6241 cgcgagccgc ctcgttcacc atgtcggccc gcgtgatgac cgaaacccgg atctcgctcg
     6301 tcgagatcat ctcgatgttg atgcccgcat ccgccaacgc ctcgaagagc tgaagagaca
     6361 ccccgatgtt gctgcgcatg ccggcaccga cgagcgccag tttaccgatc tggtcgtcgt
     6421 actgcagcgg gccgaagccg agctcgtgcc gttcggcgtt caacgcggtg acgacccgct
     6481 ggccgtctgc cttcggcagg gtgaacgaga tgtcggtcgt gccggtcgtc gccgccgaga
     6541 cgttctgcac gatgatgtcg acgttggcgt tcgccttcgt gacgaccttg aagatgcggg
     6601 cggccgcgcc gggcacgtcg ggcacgccga tgacggtgat cttggcctgc gtcaggtcgg
     6661 tggcgacgcc cgcgatgatc ggctcttcca tggacttgcc cttcttgtgt tttgccgcga
     6721 tgtaggccgc gtactcctct gacggatggc tgacgacgag ggttccttcg tcgttgctga
     6781 acgaggaacg cacgcgcagc gtgacgccgt tgcgacgcgc gtactcgacg gcgcggatgt
     6841 tcaagacctt cgcgcccgaa cccgccagct ccagcatctc ttcgctcgtg atgcgcgaga
     6901 gcttcttggc cagcggcacg acacgcggat cggcggtgaa gacgccgtcg acgtcggtat
     6961 agatctcgca cacgctcgcg tcgagcgcgg cggcgagcgc cacggcagtc gtgtcggagc
     7021 cgccgcggcc gagggtcgtg atgtcgtgcg tgtcgcggtt gaagccttga aacccggcga
     7081 cgatcgcgat cttgccctcc gagagcgcgt ctttcacccg gccgggcgtc acgtcgacga
     7141 tacgggcggc gccgtgctgg gcggtcgtga tcatgccggc ctggctgccc gtgaatgaca
     7201 ccgcgtcgtg gccgaggctg cctatcgcca tggcgagcag ggccatggag atccgttccc
     7261 ctgcggtcag gagcatgtcg aactcgcggg ggtcgggaat cggcaccacc cggtgtgcaa
     7321 ggtcgatcag atcgtcggtg gtgtcgccca tcgccgagac ggcgacgacg acctcgttgc
     7381 ccgccttatg ggtctccacg atccgcttgg cgacgcgttt gatgctttcg gcgtcggcga
     7441 ccgacgaacc accgtacttc tgtacgatca atgccacaaa cggctccaag gtgagaacgg
     7501 caggctgtag gcgacggcgc gggccgacgc gacgaacgga tgctgaccat tctagaaggc
     7561 cgccgacacc gcaccgtcgg cgtcacaccc gccgaggccg ggcggtggcg ggcagccgag
     7621 cctcggggcg cgggagggcc gggagcacag ccaggggccg gcctctgggg cgcgggaggg
     7681 cagccgagcc tcgggtcagc cgacgacgcg acggccctcg aacgcgcggc cgagcgtcac
     7741 ctcgtctgcg tattccaagt cgccgccgac gggcagcccc gaggcgagcc gcgtgacggt
     7801 gaggccgagt ggggcgagca tgcggctgat gtaggtcgcc gttgcctcgc cctcgagatt
     7861 ggggtcggtg gcgatgatca cctcggtgac ggagccgtcg gccaggcgct tcatgagttc
     7921 gcgcagccgc aactcctccg gcccgatgcc gtcgatgggg ctgatcgcgc cgcccaacac
     7981 gtgatagagg ccatggaact cgtgcgtgcg ttcgatcgcg gcgacgtctt tggcctcttc
     8041 gacgacgcag atccgcgacc gatcacgtcg cgcgtcgcgg cagatcgcgc agcgctcttg
     8101 ttcgctgatg ttgccgcact cctggcagaa gtgcaccttg tcgcgcaggt cgagcaggat
     8161 atgcgcgagc cggctgacgt cgaagccctc ggtctgcacg atgtggaagg cgatccgctg
     8221 ggcagacttc ggcccgatgc cgggcagccg cccgagctcg tcgatcaggt cttgcacgat
     8281 gccctcatac acgactcagt cgcctccgtc gagattctgt tcttcgagga accgggcccc
     8341 gagcacctcg cgcacgaccg cctcgccgta ccgttgccgc tcgtcggggc gacggaacgc
     8401 gacgccgccc cgcgcggggg ccgcggccgc ctcgtgcgcc cgagaaccgt tctgaagggc
     8461 gccttctgca ggcgacgtgc cggccgaggc accggcggcc cccggcgcgg aggcctcttc
     8521 ctgttgcccg tgcctgccgg gcgccctgtc gccgacgggc gccttcgcag gagtctcgtc
     8581 gaaggcggca tcctccggcg gctcctcctc gacgaggtcg tcttctgggg actccccttc
     8641 gcccgcgtcg ggcccggcag ccctctctgc ggagtcttcg gggtgcggta tcgcgatcac
     8701 gtcccagctc gtgaccgctt cccccgcggc cgtctcccct gtcacgcccg ggcccgtggc
     8761 acccgggtcc gcgacggtgc cgcccgtggc ggtgcgaacg ctcggccgct ggcggtcacg
     8821 cgtgacggta ccgggcacga cagccgtgtc ggcagactcg gaagcggcgg gtgcggcggc
     8881 tttcgccacg aggcccgggc gaataccgag cacggcgtcg agcgcgtctt tcaagtagtc
     8941 gcccacgccg tggccggcga cgcccgcgtc tttcagggcc tcgacgtcgc ggcgactcgg
     9001 gaacgagagg acgagtaccg gtcccgaacg caactcttgc acctgggcgg tctgcagcac
     9061 gatccacgcg ctgcgccgcg cctcttgcac gacggccagc acctgcggcc aggcgtcgcg
     9121 cacctgctgc gcggtcaccg tcgcggggtc gcgggtcgcc ggcgcgtcgc gcggcggggc
     9181 ggcagcgccc ggctccgcac cagcaccggc accggccgag cgccctcgct ccgcacctgc
     9241 cgcatgcgtg cggtcctcgt cggtcacagc cgcgccctcg tcggcgtctg gcctggccga
     9301 ggtgcccccg ccgcctgtga caggggcggg cggcgtgctg cttgccgtgg ggggtgccat
     9361 ccgcccctcc gccgccgggc cgccgatgtc gagacggcgt tccaggcgtt caaggcgcgc
     9421 cacgacgtcg cgggcggccg cgccggcggg cgcgatcagc agacgtgcca tgaggagttc
     9481 caggtgcatg cgcggcgccg tggcacccgt catagcggtc aacgccgcat gtacgaggtc
     9541 ggcgctgcga gagagctcgg ccgggccgaa ccgcgacgcc tcctgcgcga tgcgttcgag
     9601 cgcgtcgcgc ggaacgccgt gcagcacccc ctccgccttc tcggcagccg tcgccgtcac
     9661 gacgatgagg tcgcgcagcc gctccagcag gtcttccacg aatcggcgcg ggtcttgacc
     9721 cgtctgaatg acacggtcga ccacctcgaa cgcgctcgcc gcgtctcggc gcgcgagggc
     9781 cgagaccacc tcgtcgagca gcgccgccgg ggtatagccc agcagggcga cggcgcgttc
     9841 gtattcgacg gtgtcgccct cggagcctgc gatcagctgg tcgagcagcg acagggtgtc
     9901 gcgcggcgag ccgccgccgg cccgcgcgac gagcggcagc acaccggggg cgacggtgac
     9961 tccttcgtcg gcgcagatcg tgccgatgta ctcgatcatc ggcgccgacg gcacgagtcg
    10021 gaacggatag tggtgggtgc gggagcggat cgtgccgatc accttgtcgg gttccgtcgt
    10081 cgcgaagagg aagacgacgt gctcgggcgg ctcctccacg atcttcaaca gcgcgttgaa
    10141 accctgcggg gtgaccatgt gcgcctcgtc gaggatgaag atcttgtagc ggtcacggga
    10201 gggcgcaaaa atggcccgct cgcgaatctc gcgggcgtcg tcgacgccgt tatggctggc
    10261 cgcgtcgatc tcgaccacgt cgagcgagcc gtgcccgccg cgccccagct cgacacagct
    10321 cgggcacacg ccgcacgggg tgtcggtggg gcccttcgcg cagttgaggc agcgggcgag
    10381 gatgcgggcg ctcgtcgtct tgccgcaacc gcggggcccg ctgaaaaggt aggcctggcc
    10441 gacccggttg gtgcgcagcg ccgtcatgag cggttcggtc acctgtgcct gcccgatcat
    10501 ctcggcaaac gtctcgggcc gataacggcg gtacaaggcg gtgaccacgc gtccatgcta
    10561 ccgagcaaag gccgcgcggc ccggggtgtg gacaccgccg cccgaggccg gcgacaccct
    10621 ggggaaaagc ctcccgaggc cgccccggag ccgaaggccc gggccctcgc agcacccgct
    10681 caggctgccg cgagccgcag cgtctgccgg gcgatctcca gctcttcgtc ggtcggcacg
    10741 acgagcacgg ccacgcggga gtcgtcggcc gagatgcgcc tgacgcctcg ccccggggcc
    10801 tcgttgcgtg ccgggtcgac gacgatgccg agctcgtcga gcccggccag gctcccggct
    10861 cgcacggcgg cgttgttctc gccgatgccg gcggtgaagc ttatgacgtc gaggtgcccg
    10921 agctgggcgt agtaggcgcc cacgtagctt ttcagccggt gcaggtagac ggcgagcgtg
    10981 tcggtcgccg ccttgtcgcc ggctgccgcg gcggcctgaa cgtcgcgcat atcgcggtga
    11041 ccgctcatgc ccagaaggcc gctgtgcttg ttcagcaggt cgtcgaggcc gtcgacgtcg
    11101 tagccgccgt ggcgttgcag gtaaaggagt accgccggat cgatgtcgcc cgaccgggtg
    11161 cccatgacga gcccttccag cggggtcatg cccatcgagg tctcgacgct cagcccgccg
    11221 tcgaccgcgg tgacggaggc gccgttgccg aggtgcagca cgagctgttt cacctcggcc
    11281 gcagggcgcc taaggaaccg cgccgccgcc tcggagacgt acttgtgcga ggtgccgtga
    11341 aacccgtagc ggcggatgcg cagccgcccg gcaagatcgg gatcgatcgc atagtggtag
    11401 gcggccggcg gcaggctctg atggaaggcc gtgtcgaaga cggcgacgtg cggcaggccg
    11461 ggaaagaggc tccgggcagc gcggatgccc tgcacgttgg ccgggttgtg cagcggggcg
    11521 aggtcggcga gatcctcgat gccctgctcc acctcgtcag tcacgagcgt cggctcgacg
    11581 aaacgttcgc cgccgtgcac gacgcggtgc ccgaccgcca cgggcgggtg ctcgccgagc
    11641 gacggcccgt gtgcggcgaa cgcgtcgacc atcacgcgga aacccgccgt gtggtcggga
    11701 atcggaaggc tccgctcgaa cgtctcggca gtctcgcccg ctccctcccg cgtgtggctg
    11761 gcgtggccgc cgtcttcgcc gatgcgctcg acgaggccgc gcgcgagcgt ctcctcacga
    11821 tccatgtcga gtagctgata cttgaacgac gacgaaccac tgttgatgac gagtaccggc
    11881 ttcaccgatg cattcccttc acggccggct gcgcggcggc ctggatggcg gttatcgcga
    11941 cggtattcac gatgtcttcg acactcgcgc cgcgagacag gtcgttgatg ggtttgcgca
    12001 aaccctgcag cacgggaccg atcgcgaccg ccccggcgct gcgctgcacc gccttatacg
    12061 tgttgttgcc cgtgttgagg tcggggaaga tgaagacggt cgcccggccg gccacgtcgg
    12121 agccgggcat cttcgcggcg ccgaccgccg cgtccgcggc cgcgtcgtat tgaatggggc
    12181 cggccaccgg gagctcgggg cggcgctgcc tcacgagggc ggtcgcctga cgcaccttgt
    12241 cgacatccgc cccgtgaccg gattccccgg tcgagtagga gagcatggcg acgcgcgggt
    12301 cgatgccgaa ctgcgcggcc gtctcggcgg cggagagggc gatgtcggcc agctgttccg
    12361 cgctcgggtc ggggatcacc gcacagtcgc cgtagaccag cacccggtcg gcgagcgcca
    12421 tgaggaacac gctcgaggcg acggagacgc cgggcctcgt cttcacgatc tcgaacgccg
    12481 ggcggatcgt gtgggcggtg gtgtgcctgg cgccggagac catgccgtcg gccagcccca
    12541 tatgcaccat catcgtgccg aaatacgacg ggtcggtgat ggtgtcggcc gccaggtctc
    12601 gggtcgtgcc cttgctggcg cgcaggcgcg catactcgtc ggcgaacttg aggctcagca
    12661 cgtggtcgaa cgggctcagc acgtgggcgg ccgagatgtc gaggccgagc tcgatcgcgc
    12721 gggaacgcac ctcgaagatc tcgccgagaa tcgtcaactc ggcgacgccc tgggcgagca
    12781 gccgggccgc ggcgcgaagg atgcggtcgt cgtcaccctc tggcagcacg atgtgccgac
    12841 gatgggcgcg ggcccgggcg atgaggtcgt attcgaacat cagcggcgtg acgacggcct
    12901 gccgggtgac gttcagaagg tcgaggagcc ggtcggcgtc gacgtgctgg cgaaagagcg
    12961 ccagcgcgct gtcgtgttta cgctgcgaat cggccgccag ccggccgcgg gtgtcggtga
    13021 tgcgcacggc ggcgtcgtag ctggtcagcc gggtcgagat gatcggcaag gtcgaatgca
    13081 cgccgtccat cagccgcacg atcacgtcgg gcaggttgaa gccgccgttc aagacgatgc
    13141 cggccaccga ggggaaagtt cccgaggcgt tcgccatcaa gacggccaga agcacctcgg
    13201 tgcggtctgc cggcacgatg acgacggcgc cttcgatgag cctcggcagc acgttctcca
    13261 gcgacatggc cgcaatcacg accgcgagcg cctcacgccc cagaagctcg gcgtcgccat
    13321 gcacgagggt gccgtcgatc gcctggcgga tgctgcgcat cgtcggcgcg acgagcaccg
    13381 ggtcttcggg cagagcccag acgacggccc ggccgaggcg ggccttcgtg acggccccgg
    13441 tctcggcgat gatctcggcg agacggtcgg ggtcggcgcg gttgacgatg accgcgatca
    13501 cctcggcgtg ctcgtcgtgc aattcggcaa gggtgaggtc ggcgacctgg cggatctcgc
    13561 cgggtgtgcg ggagggcggg ctgcccaggt gatcgcgggt ggccgccgtc acccggccgc
    13621 ccagagtgag cagcaccggc gcgccgaggt tcgcggcgac ccgggcgttg aaacgcagct
    13681 cggtgggggt accgacgtcg gtgtagtcgg agccgacgat gagcacggcg tcgtagtcgg
    13741 cctccatcgc cttgtagcgg gcgacgatga ccgcgagggc gtcgtcgggg tcggcgtgca
    13801 ccgcctcgta ggtcgtgccg acgcagcggt cgtagtcgcc ggcgagaccc tgatgttcga
    13861 gcagcatccg caagacgtag tcgggttctt cggacgaacg tgcgaccggg cgaaagaccc
    13921 ccaccttcgc caccgtgccc gccagcgcgt cgagcatgcc cagcgcgatc gtcgacttgc
    13981 cggtatggcc ttcggccgag gtgatgtaga tgctcttcga cacgttgctc cgttctgccg
    14041 atgccaaggc acccggatcg tgtcacggtg ccccgacttc tcgcgcaccg cccgcgtgag
    14101 cgtgaggcgg cccctcacgc acccgccaga gcccgactac ccttgctgcg tttccgccct
    14161 gggggagttc gcctggatgg cgccacgtga ggagccatac acagcatatg cgaccgggcc
    14221 cgcgcggcac acgcccgcgg gccttcccgg cgggcgaaaa cacggcgagg cgtaagaata
    14281 ggcgtgaggc acgcgtgtcg tgaccgccgt cgaggaggaa caccatgggg catttgccgc
    14341 cgttggtcga gcccggaccc gagctttcgc tcgagcgtct catccgctac agcaggcagt
    14401 tggccctacc ggggttcgac gagaccgcgc agcgcaggct cgcagccgcc cgcatcctcg
    14461 tcatcggcgc cgggggcctc ggcagcgcga gcatccccta cctggcctcg agcggggtcg
    14521 gcaccatcgg tgtggtggac gacgacgtgg tcgagctctc gaacctgcac cgtcaagtga
    14581 tgcacaccct cgcgatgctg ggccggccga aggtggagtc ggcggcgctc gccgccctgg
    14641 cgatcgaccc cgagatccgg gtcgagaccc accccgtgcg ggtggatgcc acgagccttg
    14701 cggcgattct cggcgactac gacctcgtgc tcgacggcag cgacaatttc gagacccgtt
    14761 acctgtgcgc tgacgcctgc gccgcggccg gcaagcctct cgtgtggggc gcgattctgc
    14821 gcttcgcagg ccaggtgtcc gtgtcgtggg cgggcgccgg cccgacctac cgcgacctct
    14881 acccggtgca gccggcggca ggcgaggtgc cgaactgcgc gcaggccggc gtcgtgccgg
    14941 gcgtgtgcgc cgtcatcggc tcgctgatgg cgacggaggc gctgaagatc gtcacggggc
    15001 tgggcgagcc gctcgtcggc cgggtgacga cgtacgacgc gctgaccggc cggttccgcg
    15061 agatcgagta cgcggccgcc gatgacgagc ccaccgtgcc cggtaccggc ggccgggacg
    15121 ccgaagacgc gcggccggca cgtgccgcgg ccgccgggac ggtcgccgtc ggggcagcgc
    15181 cggcacccgc cgtgccggcg acaccggtcg gcggtgcgac gagaccccgg ggccgggcct
    15241 cccacccggg ggcggcggcg gccacaccgg gcaggcaagc ggcctctttc aacccggcac
    15301 gcgcgcagat ctcggtgcgg cagatggccg attggcgcca acaaggaacg accttccagc
    15361 tggtcgacgt gcgggagccg tacgaggtgc gaatcgccgc gatccccggc tctgaggcga
    15421 tcccgttgcg gagccttggc gacgagctcg gccggctgcg ccacgacgtg ccgctcgtcg
    15481 tgcactgcca cgtcggcgac cgctcgttcc aggcggtgcg ctacctgcac gcgcacggct
    15541 acccgaacgc cgtcaacctg gtgggcggca tcgacgcgta cgcccggcag gtcgacacca
    15601 cgatggcgag gtattgagcg atatggaaag tgtcatgggg gtgcagagcc tcgtgcggct
    15661 ctctcgtatc gtgagcgagc cgatcgacga agacgcggtg cgcgacgcgg tggcggccga
    15721 cgagaacggt gcgacctgcc tgttcctcgg cgtcgtgcgc aatcacgacc gaggcaggaa
    15781 cgtcgttcgc ctcgactacc aagcacaccc cgacgccgaa gtggtcttgg ccgcgctgtg
    15841 cgccgaggtc gcgcaggcga ccggagtcgc cgtcgcggcg gagcaccgca caggcaccct
    15901 tgtcgtcggc gacacggcgc tctgcgcggc ggccggctcg ggccaccggg cagaggcctt
    15961 cgacgcctgc cgcatcctcg tcgaacgcat caagcacgag gtgcccatct ggaagaggca
    16021 acacggcgcc gacggcacca cggagtgggt gggtctttaa actttaaagc tggtcggcgg
    16081 gcccccacgg caggaggatg ctgcccgtgg cggtgcgcgg ctcgccgccg acggggacca
    16141 cgatgaccgg ccggccctgc cggtgggcca ggcgcacggc gaccgagccg ccgaagaact
    16201 cctgcattcc ggagcgaaag ccggccctgc gggttcccac gatgatcgcc tcggcgtcca
    16261 gcctctcggc cagatgcgac agggccacgg ccggttcacc cgcgaggagc cgcgtgctcc
    16321 accgaaccgg gccgccgtcg aaggtcgcag ccagcctcgc ccgaagcccc tcgtcgaaga
    16381 cctcgtcgcc ctcctcgacg acgtcggggt cgacggacga cgagacgacg gaaccgtcga
    16441 ggccttcctg caccacgtaa cgcatcgcgt ccacgtgggc acacaccaac tctgcgtcga
    16501 acagattggc gagacgcgcc ccggcgagcg gcactttctc cgactggccg ggcacgaccc
    16561 cgacgatcaa gaatcgatcg gccgcctgcc cgcccatatc gccctccgag ccccggtgca
    16621 gcgttcccca cagggtagcg ccggatcagc ctccggcgaa ggggggaagt acatcgacac
    16681 ggggggcgag cggcatgtcg aggctgcgcc gcacgacctt gtcgatgagg aaggaaccgt
    16741 tgcgcaagac atcggtcatg cccgtgtagc gcttttcgag caggctgcgc aggtcgccca
    16801 gcgtcgcgtt cggcgcgaaa tgcaacgact cctcctcgcg gccggccgcg tcggccgccg
    16861 ccgcgaagta ccgcaccaag acatccatct tcgccatcct cccacgcccg gccgaggccg
    16921 gccggcgcgg ggccgctgcg gcggccgtca ccgtacgggc cgcactccct cgggcacctg
    16981 cacgacgacc gccgtcgatt tgacggcggc caccgcgacc gaaccgacct cgaggccgag
    17041 atcgtggcac gactgcgtcg tgatcacgga gacgacccgg tgcgggccgc attgcaattc
    17101 gacctggcag acgaccggcc ctgtgacgat tttcgtcacg aggcccacga agcggttgcg
    17161 ggcgctcgtg accccctcca acgggtcgac ggcgggcgcc gcgatgcgct ctgcataggc
    17221 ggccagatcc gtgccctcga ccgcttgacg ccccgacgcg tcggtcgtca ccggaaggtc
    17281 gccgctggcg ctccagcgcc gcaccgtgtc gtcgctgacg ccgagcagcg cggcagcctc
    17341 gctgatccga tactgcgtca taccgcacat cgtattccgc gccaccacgc gggaggatgg
    17401 ggggccgtgc ccggccacga ggcctcgcag tagagtgcag ccatggcaga cattccggcc
    17461 tcggtctccg tgcgtgagca cctggagcgc gtcctcacgt cgatcgaacc cctgctgcag
    17521 acagacgctc ccctgtcgcg tgcccgcggc agggttctcg ccgaagacgt gcgggccctg
    17581 gtcgccatcc cgagcttcga caactcggcc atggacggct acgccgtgca ccgcgacgac
    17641 gtcgccgagg caagcacgct acgaccggtc acgctgcggg tggtcgccga cctgccggcc
    17701 ggcactgccg aggaccccgc cgtagggccc ggcgaggcgg cccgcatcat gaccggcgcc
    17761 ccgctgccaa gcgacgcgga cgcggtgatt cccgtcgaag acaccgaccg gggcctgccc
    17821 gaggtcgtcg tgacggcgcc ggccacgacc tccggctatg tgcgagaggc gggcgccgac
    17881 acgcggatcg gcgaagtcgt gttgacggcc gggcgcattc tcgactccca agacatcgcg
    17941 actgcggccg cgaccggctc ccgaaccctg ccggtctttc ccgcgccgcg ggtggcgatc
    18001 ctctccaccg gcagcgagct cttgacgccg ggcgagccgc tgacgcgcgg ccagatctac
    18061 gacgcgaact cgtatctgct cgaggcccag gtcgccgagg ccggcggggt gccggtgcgc
    18121 atcggcgcca ttcccgacaa cctcgaccgg ctcggcgggg cgctcgacga gatcgtgcca
    18181 ggcgtcgacg cgatcatcac ctcgggcggc gtcagcgtcg gcgcctacga cgtggtgaag
    18241 gcgcttctgt cggggcacgg caacgtctgg ttcggcaagg tgcgcatgca gccgggcggc
    18301 ccgcagggtt tcggccactg gtccgacggc accccggtct tcaccctgcc cggcaacccg
    18361 gtcagctcgt ttgtgtcgtt cgaggtctac gtgcggcccg ccctgcgcaa gatgcaaggg
    18421 ctgacgccgt gggaacgccc cgtggtgcgc gcggtcgccg cccaagactg gaccgggccg
    18481 gccgggcggg cgcagtacat gccggtcgcg ctcgtgcgcg gcgaggccga ggaggtgccg
    18541 ctgcacgtgc ggccctccgc ccggggaggc tccgcctccc acctcgtcgc gagcctggcg
    18601 gcggcagagg gcatcgcgat cgtgcccgaa gccgtcacgc tcgtcgaggc cggcaccaac
    18661 gtcgacgtga tgctggtgcg caactgatgg ccgcgccgaa gttcacccac gtcgacgcac
    18721 gcggcgaggc caggatggtc gacgtcaccg cgaagacccc gaccgtgcgg gccgcgacgg
    18781 cgagcgggtt cgtctggtgc gcgccgatcg tcatggcggc gctgcgcgac ggcgagaccc
    18841 cgaaaggcga tgtgctcgcc gtggcccgca tcgcgggcat ccaggcggcg aaacgcaccg
    18901 ccgaactttt gccgctcgcg cacgtcatcg gcgtgcacgg cgtcgtcgtc gacctcgcgg
    18961 tagaagaaga aggggtgcgc atcacggcga ccgtgcgcac tgccgaccgc acgggggtcg
    19021 agatggaggc gttcaccgcc gtcagcgtcg ccgcgctggc gatcgtcgac atggtgaagg
    19081 gcatcgaccg cacgagcgag atcgggcaga ttcaccttga ggcgaaagag ggcggccgca
    19141 gcggcacatg gcgacgctcg acgcagtgag agtcagaccc tcagtggccg tggtggccgt
    19201 gagcgtcgtg gtcgccgccg tcgagctggt cgatcacgtg cgcgacgagc ggcaacacga
    19261 cctggagccc ctccgcgacc gcgtcgggcg agccgggcag gttcacgatg agggcccggg
    19321 tgcgctcgtc ggtgacgccc gcgatgccgc ggctgaggag cgccgtcggg tgcatcacag
    19381 cgccggtgcg gcgcatcgcc tccgcgatgc cgggtagttc gtacgccacc acctcgcggg
    19441 tgccctccgg cgtgcggtcg cgcggcccga ccccggtgcc gccggtcgtc acgatcagcc
    19501 gcgcgccgtc ggcgagggcg gcgcgcaacg ccccggtcac ggagtcggcg ccgtcgggca
    19561 cgaccatcgt gtcggcagag tagccggcct cggccagaat actcaccgcc cgagggccgg
    19621 agaggtcgtc tcgcagccct tcggaggctc ggtcggagac ggtgatgacg gcggcggaga
    19681 tcttttccgc ccggtgatgc ccgtcacgat gggtgtgcgt gtcgccctgt tcctctgtca
    19741 cctgagtcat gcccttaatg tatgccggca cgctgcaccg gctcccagcg aacgtccatc
    19801 tcatcgcccc gcgtggccgc accccactgc cccacgcccc gtgcgatggc catgccaccc
    19861 cgctgtctcc cctgccgcga tcactcaggg cgcgagcgcc tggggccgtg ccccacctgg
    19921 cgttccgcta ggatactgag ggtcgtcgca ggccggattc ggctgcgaga ccgctggagg
    19981 attcgcctag tggcctatgg cgcacgcttg gaaagcgtgt tgggtgcaag ccctcggggg
    20041 ttcgaatccc ccatcctccg ccatcgattt agtgcttctg atcaggtatt tagcactctc
    20101 atataagcct gcgacggggc ttatgtgctg ggatatgtgc taagcctcgt caaggagcgc
    20161 ggcgaagcgg ttgaccgcgg ccttctgcat ggtcggcgtg acgtggctgt agatgttcat
    20221 cgtcgtggtg atcgtggaat gcccgagccg ttcctgaacg accttcggat gctctcccaa
    20281 ctcgagcagc agcgtcgcat gcgtgtgccg aagtcccttg agtgtgattc gcggcaggcc
    20341 gtcgaccttc tcctgcgctc gcttcacgcg gtaggtccaa cgccgaccga cctcgttggg
    20401 tgaacgaatc gcgcccgcgt cgttgccgaa cacgtaggca tcggcgcgcg caaggtcgag
    20461 tgagacagct ccgcgcttgg cgcggtagcc cctcaggacc gcgagggtgt ccgcatcgag
    20521 atcgagaaca cgtgcggccc ctgtcttggt ggtcttggtc ttgttccgcg tggtcacatc
    20581 cgcggcacgt cggatgctga gcctgctggt gccgaaattc acgtcacccc aacgcagggc
    20641 gagggcttca ctgcgtcgca ttccggtgta ggcgacggtg cgccagagcg ggaagagagc
    20701 atcgccgagc acgtcgcgat tccatgtcag gaaggagtgc agctgggcgc cggtccaggt
    20761 cgcgatctcc ggccgctggg cccgaatctg tgaccccgta ggtgcgttca cggtgcgctt
    20821 tttccgcgct gggttgaccg cgatcagacc gtcgtccagc gccgcgtcaa ggattgcacc
    20881 aagtacgacg tgcaccttgt tgactgtatt aaccgagagc ggttgaccga atccgcgctt
    20941 gtctcgtcgc cctgacttct ccagctctct gtaatgccgt ccgattctgg ttgccgtcag
    21001 cttgtcgagg cggatcgcgc cgagctgagg cacgatgtgg ttacggatga tcttttcgta
    21061 gccctgcacg gtcgaggact ccaggcgcag gcctttcgtc cattccgtcg cataggttgc
    21121 gatagtgggc accttgccct ggaacttctc gttccgcgca cgcttcttca gtgcttcggc
    21181 gagtgcggct tgggcctctt caaggtcagc gaacccgcca cggctgacgc gagtctcgcc
    21241 gagttcaggg ctctccggat ctttcagaac gtagatctgg aacttccatc gcttgccctt
    21301 cttcgtctga tagtcggcca gtgatcctgc gtgccgcgat cgtgaccggc gctgtcttct
    21361 cgcctcctct ggcccgtcta ctgtgccgtc gggcggtcgt gcggtctgtg ccatgatccg
    21421 gctccttccc ttgccttggg tgtcgtggtc tattcgttga agagtgaggc gttcgggtcg
    21481 agttgtagga gccggcccgt ggcgtgggcc tcgcgttcgg tctgccggtc gtggcgctcc
    21541 tggtatacct cgcgcggggt gacaccgacc tggccgcggt cgtacttcgc cagcatctcc
    21601 tcggcccagc gtgtggagtt gatcgcttgg aaccgttgct ctttccagta ccgagccagg
    21661 tcctcggtgg tgagcttcac ggcgccggcg atccacgcct ggatctcttc ggcgaggaac
    21721 gattccggca ctccggtcag cgtgaccgcc tcggacggtg gggtgaggag tgccgcggga
    21781 gtggtccgga gaatataggc aagggcggtg aggtcgtcca cgtccacgcg gcgatcgccg
    21841 ttctcgatct tgctgatgct cgacgcggcc agcttccggc cggcctcggt catcaagcgt
    21901 gcggcggtgc gcacatccat cccgatcgcc tggcgtgcgg cgcggagatt cgcggcgacg
    21961 tgctcctgcg tgacgccagc cggattacct ctcacccgtt ctgttcccat actagaaacg
    22021 tatataccta tgtttgactc gtcaagttct ctgttgtatt tttagaatcg tacgacgctt
    22081 tacaagaaac ggtggtggtg atgcgaactg tgacggtgac cttagacgag ttgcgggtct
    22141 cgcggaccgc ggtgatcacc cgagctgagg cagccgccgc gctcggggtc gatccccgca
    22201 cgatcacggc gagcattgag aacgggtcga ttccatccgt gcgcctcggc aggcgagtcg
    22261 tgatcccgcg cgagaagttc ctgcgcctct tcgacgccga gatctcaccg cgcagcggca
    22321 ctgacggcgt gtcctcgatg tgaggcgccc tgccgtgccc cttcggcacg tccgttctgg
    22381 ggatcgtcgg gatgccaagg taaaatgaca aatggagagg gacggagaag cgcatgagtg
    22441 agaccaccgt gactcggcgg gcaccggcac cgtacacgcg tcacaacttc gatcatgtct
    22501 ccgatgtgat gctctacgac atcgcccggc acacagcctc ggtgctctcc tccgtgctgc
    22561 tgaacctcgc cgacggggcc accgacgacg cggaacgcgg ccattgggcg gcacggcggc
    22621 gtctggtgaa gcagcagatg agggccctgg acgtgcacga ccgggccggg ctgatcgcgc
    22681 aggacgaggt gtggcggctg gaaaagctcg ccctcgccgg gaacgcgtga accccgagcc
    22741 ggtcggttca cccgcttggc tggcgcgcgt gtttgccaca actgtcgtcg acgacctgtt
    22801 cgccggccac acacccggcc gtggtgaccc cctcctggtg ttgctgggtg ggcaacccgc
    22861 ggctgggaag acgcaggcac aggccgcgat cctggccgag tgtgccgccg atgacctggt
    22921 agagatcacc ggcgacgacc tccgcgaata ccacccggac tatgcacgcc tggccgaaca
    22981 gtccccgttc gcgatgccag gcgtcaccgc accggtttcc ggcggcctca ttggcctagc
    23041 gctggatcac gcactcacgc aacggcactc ggtcctgtta gagggcacgt tccgcgataa
    23101 agagatggtg attgcgacag cgacccggtt cgcggacgcc ggttaccgag tgatgatcgt
    23161 cgccgtcgcg accccggccc cggtgtctcg gctgtccacg gagatgcggt ctctggacgc
    23221 cggctaccct gaggtgggcc ggtggacccc gccggacgcg cacgagagcg cactggcggg
    23281 ttctgcgatg gttgtgtccg cactggaagc gctccccttc gtcggccggg tgcaggtatt
    23341 ctcccgtgag gcgctgctgt tcgacaattc ccgtaccgac accggcgaat gggcacagcc
    23401 cgctcgcgcc gctcgtatcc tgcggcagga acagcatcgc cagttgagtg tgcgagaggc
    23461 atacttctgg cttcaggact atgccgccgt gttcgcgacc gcgcgagcgc gtccgggata
    23521 cttcgcctcc gagaccgctc cgacctacct gaggctccag aacgatgcgc agaacatgat
    23581 ccacattctc agcaagaagc ccggcgcacc cctcagccaa ctcagccgcg agcaacgtgt
    23641 gcgagccgcc atcttggaga gggtcctccc gccggaatca ctcccggaac cacacaaatc
    23701 cgggatgcgg tcacagcgga gccagccaca tccgagtagc cgcccagatc cgcccgtccc
    23761 ccggctctag cctgctcagc cgagcggccg ccagccatca ccccgggacg gactgcacgg
    23821 gtcgccgacg cccgactttc tgcttcaatc atcagatgct gccgtcgcaa tcggatcacc
    23881 ggacggcgct actgcaaggg gtgcgcggca tgcggggcac ttccgaattg gagacggctc
    23941 gatattctgc gataggtatc tggatcggcc ggaggctccc gttcacaggc cgcgtgtcgg
    24001 tctcccgcgg acggtgtggc gttgtcgctg gaactcctcc cgtgtggtct gttgcctggc
    24061 cgtccgccgc gcctcacgtt cttcctcggc ggcatgggcc cgtctgatcc gttcgagggc
    24121 ctcagcaggt gcgagagtac gcaagagatc tgcctcctta cgcgtccggc cggcggtttg
    24181 tgaaaggtca gcgcagcgtt gcgccgggtt cgctttgagg aaagcgaact gatcgcgcat
    24241 taccttttcg gcaccgaaca cacggacgta taccgccagt cgagctgccc gtactcgttc
    24301 gggccggttg tagagggcgt tgcgtgcgct ctggtgggtc gcctcggctt cggcgaggcg
    24361 tgggtcagcg ttgatcacgg gtgtggtgac ccgttcgacc caggcggtgg ggcgctcggt
    24421 cggtttcggc acaccacccc acgctcgggt caatcgttcg cgtacatcgc ggacatggga
    24481 tcgcgcgttt tcgtgttcgg tgcgggcgcg ccgcttcctg agccacgatg cgtccgcgat
    24541 cttttggagt gttgcttgtt cgtgggcgtt ggcatcgcgc cactgtgcga ctgctgcctt
    24601 ggcgtcggcg aggatcggga ccacgatctc ggcccgtacc cgttccacgg catgccgggt
    24661 ctgtcgtccc ctggaggctg cctgcgcgcg cttctgtgtc tcctgcgcag tcagttcggt
    24721 aagagcagtg tcggcctgct gccagagcgc tgcccgagcc tcggcgactt cggcgcgccg
    24781 tagcagggtc tcgatctgat tgttgatgaa cgtgaccggg ccattctcga tcagcccggc
    24841 gacgtcctcg cgcgcatggg cggaggcgac gtcgaggccg cggtcggcac ggtctcgtcc
    24901 catcgcatcg atgaattgtg cgcgcgcgtc ggcgatgtcc tccgcggcga tgtgcagcac
    24961 gttcgaggct cggccacgcg tcatcccgac gtagacgccg gctgcggtcg tcgtgtcggt
    25021 caggagggtg tgcgcgccgg tcacggtcgc gccctggatg ccgtatgcgg tagtcgcata
    25081 ggagaggtgc gtgtgttctg tgacgtactc agccggcaga tgcacggtgc gctgccgctt
    25141 ccgaccggcc cgggcctcac gaactctgag agatccgtcg gcttcgacat gttgcacggt
    25201 ccacgtctgc cggttcgcga cccccagttc ggagttgtta tggcgggtct ggatcaggtc
    25261 gccagcgccg atggagagcc cgtcgttgcc gaacaccacc cgggtgtcgt caacaagacc
    25321 tctgacgacg cgtacttcgc ggatgcggtc attgagagtg cgcgcatcat cgttcgtggc
    25381 ggccgtgatc gcctcgcctt cccgccactc ggcggcgatc gcgtcgcgcg cgtcctcgtc
    25441 gctgtcatgc agccggatga ggcctgtctc gtttagctgg tcgaacacgt caccgggatt
    25501 ggtgccgtcg cgcatccgca tggtgacggc cgcatacgcg cggtcggcaa aacggtgcac
    25561 ctcggccata tcgaaagtgc ggccacggat atgtgcggcc atgtcgagca ccccgccgcg
    25621 accgaccgcg gggagctgcg cgcggtcccc gacgagcgcg accgttgccg cggcggtatc
    25681 gcacacggtg aatagggcga tcgcggtgtc ttggtcgagc attcctgcct cgtccacgat
    25741 cacccgctca ccc
//
LOCUS       DASTNL010000213         9781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_712173, whole genome shotgun sequence.
ACCESSION   DASTNL010000213 DASTNL010000000
VERSION     DASTNL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712173"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..510
                     /gene="gatC"
                     /locus_tag="VFQ96_06850"
     CDS             214..510
                     /gene="gatC"
                     /locus_tag="VFQ96_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0257539.1"
                     /translation="MPEISVEEVAHLAELARIDMTPEEIAGLTADLAQIVDAVARVNE
                     VATDDVPATSHPMPLVNVLRDDVPRPSLDVAAALSGAPEREGDRFRVAPILEEE"
     gene            513..2051
                     /gene="gatA"
                     /locus_tag="VFQ96_06855"
     CDS             513..2051
                     /gene="gatA"
                     /locus_tag="VFQ96_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU0257540.1"
                     /translation="MAGNDLTRLPAARLAELLAHGEATSVEATSAYLDRIDALEPSVH
                     AYLHVAREAALATAAGIDSRRAAGEAMGPLSGVPVAVKDVLATKDMPSTAGSRMLEGW
                     VPPYDATVVARLREAGMVLLGKTNMDEFAMGSSTEHSAYGPTHNPWDLDRVPGGSGGG
                     SAAAVAAFEAPVALGSDTGGSIRQPAAVTGSVGVKPTYGGVSRYGAIALASSLDQVGP
                     VSRNVLDAALLHDVIAGHDPLDSTSLDEAWPSMAEAARRGRGGEGLKGLRVGVVDELS
                     SEGFQKGVGARFAEALDLLAAAGAEIVHVAAPSFGYAVAAYYLILPAEASSNLARFDS
                     VRFGMRVVPTSGSLTTERVMAATREAGFGPEVKRRIILGTYALSAGYYDAYYGKAQKV
                     RTLIQRDFETAFATCDVLVSPSAPTTAFRLGEKIDDPLSMYLNDITTIPANLAGVPGM
                     GLPIGLAPEDGLPVGLQVMAPARHDARLYEVGAGLEALLEEKWGGPLLARAPRLAESE
                     GARS"
     gene            2048..3541
                     /gene="gatB"
                     /locus_tag="VFQ96_06860"
     CDS             2048..3541
                     /gene="gatB"
                     /locus_tag="VFQ96_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU0257541.1"
                     /translation="MKADLMDYDEALAKYEPVLGFEVHVELNTKTKMFCGCANEFGQG
                     ANTNTCPTCLGLPGALPQVNRQALASSIRLGLALQCSIAESCRFARKNYFYPDLAKNF
                     QTSQYDEPIAFEGSVSIELESGRTLDIPIERAHMEEDAGKLTHVGESGRIQGADYSLV
                     DFNRGGVPLVEIVTHMIEGAGSDAPEVGRTYVAAIRDIVKALGVSNARMEEGNVRCDA
                     NVSLRPRGSGRLGTRTETKNVNSLRSIERAVRYEIRRQAAVLEAGGLVTQETRHWHED
                     TGTTSAGRPKSDADDYRYFPEPDLVPVEPSRTWVEELRQTLPELPAVRRRRLLAEWGC
                     TALEFQDVANSDLVDEVEETVAAGARPDAARKWWTGEIARLANQQDAAPASLITPRQV
                     ADLVGLVDSGTLTDRLARQVLAGVVAGEGSPADVVEKRGLAVVSDDGALAAAVDTALA
                     AQPDVLDKIRGGKVQAAGAIIGAVMKAMKGQADAARVRELVLERARE"
     gene            3589..4245
                     /locus_tag="VFQ96_06865"
     CDS             3589..4245
                     /locus_tag="VFQ96_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257542.1"
                     /translation="MFIEGTLRWKVTEDCPWDLLVALCLRDMAGLADLGEPVLPAVTP
                     AVPAVAHTVLRPGRTGGTGPGSADKDGGGGHRGALRAQWLAWWARTATPGTRPYVSDL
                     RPPHFAAFDRQLELQDLIETHYVAAARWADRRREEYLRTRSERRAAFAADVVEPVRER
                     EHALRRQAGYFRLDLCVLPLAERGTWVIAPDTIAISRGLREDLPAFRAWWRRLAEVLV
                     "
     gene            4295..5551
                     /locus_tag="VFQ96_06870"
     CDS             4295..5551
                     /locus_tag="VFQ96_06870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU0257543.1"
                     /translation="MGRTDGTGRVVTTGPVDDAATTILHADMDAFFASVELLERPWLR
                     GRPAVVGRRTGRSVVSSATYEARRCGVHSAMPMAQALRLCPQAVVLEPHMEKYRAVSA
                     RVMAIFHGVTPLVEQVSVDEAFLDVAGARRLFGTPSEVARLLRARVRGETGLSCSIGA
                     SAVKFVAKLASAKAKPDGMLVVPAGQTREFLRPLPARALGGVGPATTQLLADRGIHTV
                     GDIAATPEATLVRWLGEAGGTRLARLARGVDPRGVVPGRTEKSIGREQTFEHDVTDPK
                     VLRREVLRLALDTASRLRRVGLVGRTVSLKIRFADFTTLTRSRTLPEPTDVGRRIGQE
                     ALQIVAAAGAARPVRLIGVRVENLAGPSDGALLWDPDDDWRGVESVTDTIGARFGPEA
                     VTSASLLGTRPRRVGDTSSVSRAAGG"
     regulatory      5570..5681
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5722..6471
                     /locus_tag="VFQ96_06875"
     CDS             5722..6471
                     /locus_tag="VFQ96_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU0257544.1"
                     /translation="MTNDTDEGAAHPAGPEAAGPASSDAGDDSTRGAVFDPLGGAARA
                     AHPIRRARRWRSLDIVVAAVLGVASGVIFWIWGLAWAPVSAIFALTPGLDGLTAGGWL
                     FAGVLGMLVIRKPGAAVFTELVAGFVEMAIGNSWGASSIVWALVEGGAAEIVFLIVGY
                     RHWRVWVASLAGAAAGVGTAFLDTTFSSLAGLSAPAKLLYGGCAVLSGLVVAGILTWL
                     LVRALARTGVLRRFPSGRGARIVDAAGPAAG"
     gene            6481..8094
                     /locus_tag="VFQ96_06880"
     CDS             6481..8094
                     /locus_tag="VFQ96_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257545.1"
                     /translation="MALGRTGGARVVARGWGLRYSGRRSPALVGIDLDIAAGQRVLLA
                     GASGAGKSTLLQALAGVIGDEEDGEQFGLLTVNGRPCTEAPGVAGLVLQDPESQSVLP
                     KIGDDVAFGCENMGIEREEIWRRVGESLALVGLDLPWLSATGTLSGGQKQRLALAGVL
                     AMRPGLLLLDEPTANIDPAGARRVRDAVAHAAAVTGATLLVVEHRIDLWLPVVDRMIV
                     LGEEGGVRADGSPADVLRRDGDALAAAGVWVPGALSGSSEPAARRPSAAALPGAAAGR
                     AREAPATGPTGGAPAGEPLLSTHDLAVAPGTRAEVVARPPALAVRAGDALAVTGPNGS
                     GKTTLALTLGGLLRPAAGAVRAGEALAAGLAPDPYRWRSRQLATRIGSVFQQPERQFL
                     AGTVRGEFRIGLDAARVPRKRAERITEALLDRLGLTRLAEANPYTLSGGQKRRLSVAA
                     TLALSPPLLILDEPTFGQDLRTWRGLVDLLAERLEAGHGIVLITHDEPLVAALATRHV
                     SLPAHEGVGRPAPVPAGPGGSRQNRQAGS"
     gene            8091..8927
                     /locus_tag="VFQ96_06885"
     CDS             8091..8927
                     /locus_tag="VFQ96_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU0257546.1"
                     /translation="MTTATPPGTSDGQLTAARLHSPDATWPARRGVDPFAGLVAALIL
                     GLSLVVTLDIASAATALVLELLFLPWVGASGRAWLRCTFLLAAACLAALTTALYGEVA
                     GHIYVRWGILTVSSGSLTLAAAIALRVLAVGVPAVVLLARVDVTRLADALAQIVHLPA
                     RFVLGALAGMRLAALFVDDWRRLSLTRRARGLGGGGPVRRVRRAAWQVFALLVAAIRR
                     GTVLAVAMEARGFGRAAGRTWARPARLTARDVTVITLAAAIAAAAIVVSLVTGSWNFV
                     LA"
     gene            8929..9537
                     /locus_tag="VFQ96_06890"
     CDS             8929..9537
                     /locus_tag="VFQ96_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0257547.1"
                     /translation="MSVPGVVDDLLRDALRRRRPDGAPATVLIDGPAGAGKTSLAAAI
                     ARRWPAYLSAAVPASPPLVLLHMGDLYPGWEGLAEGSRTLAERVLLPRSHGRDGRFQT
                     YDWVAARPGPWRTVPASAPLLVEGCGALSRAAAALATLRVWLDADEELRWRRVSRRDG
                     GAFDDEWPQWSRQMVSFITREQPRRHADLTLRSDEQGVIRPR"
     gene            complement(9563..>9781)
                     /locus_tag="VFQ96_06895"
     CDS             complement(9563..>9781)
                     /locus_tag="VFQ96_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEU0257548.1"
                     /translation="DLLGLYEGVALTRRGDYGFGDLPDRILLFRLPLLRAGGDLDGVR
                     REVRITLVHEIGHFHGLDDDRLHELGWA"
BASE COUNT         1330 a         3498 c         3489 g         1464 t
ORIGIN      
        1 ctcgacgccg cacagttccg gcggctcctt gccgaggggc cagacgccgt gcgggaggcg
       61 agcgcgtgac ggggatcgtc acgcaggcct gcggcctggg ccgtgcgccc tcctgcgttg
      121 tggactctcg ctcgtacggg ctccagggct tagggcgggc agacggggcg aatagacttg
      181 tgccgtccta tgcccggcga tcgcggaaga agcatgcctg aaataagtgt tgaagaggtc
      241 gctcacctgg ccgagttggc gaggatcgac atgacccccg aagagattgc cggcctgacg
      301 gccgacctgg cgcagatcgt cgacgcggtc gcgcgggtca acgaggtcgc caccgacgac
      361 gttccggcca ccagccatcc gatgcccctc gtcaacgtgt tgcgcgacga cgtgccgagg
      421 ccctctctcg acgtcgcggc agctctttcc ggggcgccgg agcgcgaggg tgaccgattc
      481 cgggtcgccc cgatcctgga ggaggagtga ccatggccgg gaatgacctg acccgtctcc
      541 cggccgcccg gctggcggag ctcttggcac acggcgaggc gacgagcgtc gaagcgacga
      601 gcgcctacct cgaccgcatc gacgcgctcg agccgagcgt gcacgcctac ctgcacgtcg
      661 ctcgtgaggc ggcgctcgcc accgcggccg ggatcgacag ccgcagggct gccggtgagg
      721 ccatgggccc gctttcgggg gtgcccgtcg cggtgaaaga cgtgttggcc accaaggaca
      781 tgccctccac ggccggctcg cgcatgctcg agggctgggt accgccctac gacgcgaccg
      841 tcgtcgcgcg tctgcgcgag gccggcatgg tgctgctcgg caagaccaac atggacgagt
      901 tcgcgatggg ctcctccacg gagcattccg cctacggccc cacgcacaac ccatgggact
      961 tagaccgcgt gccgggcggt tcgggcggcg gctcggccgc tgccgtggcg gcgttcgagg
     1021 cacctgtcgc cctcggcagt gacacgggag gatccatccg ccagccggcc gccgtgaccg
     1081 ggtcggtcgg cgtcaagccg acctacggcg gcgtcagccg ttacggggcc atcgccctcg
     1141 cctcctctct cgaccaggtc ggcccggtct cccgcaatgt cctcgatgcg gccttgctgc
     1201 acgacgtcat cgccggccac gatccgctcg actccacctc cctcgacgag gcctggccat
     1261 cgatggccga ggccgcccgg cgaggccgcg gcggcgaggg actgaagggg ctgcgcgtcg
     1321 gcgtcgtcga cgaactctcg tccgaaggct tccagaaggg ggtcggcgcg cgcttcgccg
     1381 aggccctcga cctgcttgcg gccgccggcg ccgagatcgt gcatgtcgcc gcgcccagct
     1441 tcggctacgc ggtcgccgcc tactatctga tcctcccggc agaggcctcc agcaacctcg
     1501 cccgtttcga ctccgtccgg ttcgggatgc gcgtcgtacc tacctccggc tcccttacca
     1561 cggagcgggt catggccgcc acccgggagg ccggtttcgg ccccgaggtg aagcggcgca
     1621 tcatcctcgg cacctacgca ttgagcgccg gctactacga cgcctactac ggcaaggcgc
     1681 aaaaggtgcg caccctcatc caacgagact tcgaaacggc gttcgcgacc tgcgacgtgc
     1741 tggtgagccc gagcgcgccc accaccgcct tccgcctcgg tgaaaagatc gacgatccgt
     1801 tgtcgatgta tctcaacgac atcacgacga tcccggcgaa cctggccggt gttccgggca
     1861 tggggctgcc gatcgggctc gcacccgaag acggcctgcc ggtgggcctg caggtcatgg
     1921 ccccggcccg ccacgacgca cgactgtatg aggtgggtgc tgggctggag gcgcttctcg
     1981 aagagaagtg gggaggcccg cttttggcgc gcgcgccgcg gctggccgag tcggaaggag
     2041 cccggtcatg aaggccgacc tgatggatta cgacgaggcg ctggccaagt acgagccggt
     2101 gctcgggttc gaggtgcacg tcgagctgaa caccaagacg aagatgttct gcggctgcgc
     2161 gaacgagttc ggccagggcg ccaacacgaa cacgtgcccc acctgcctcg ggctgcccgg
     2221 cgcattgccg caggtcaaca ggcaggcgct cgcctcgtcg atccggctcg gtctggccct
     2281 gcagtgctcc atcgccgaaa gctgccgctt cgcccgcaag aactatttct atcccgatct
     2341 ggccaagaat ttccagacct cgcagtacga cgagccgata gccttcgagg gctcggtcag
     2401 cattgagctc gagagcggcc gcaccctcga catccctatc gagcgagccc acatggaaga
     2461 agacgcgggc aagctcaccc acgtcggcga gtccggccgc atccagggtg ccgactactc
     2521 cctcgtcgac ttcaaccggg gcggcgttcc gctcgtcgag atcgtcaccc acatgatcga
     2581 gggtgccggc agtgacgcgc ccgaggtcgg ccgcacgtat gtcgccgcca tccgagacat
     2641 cgtcaaggcg ctgggcgtct cgaacgctcg catggaagag ggcaacgtac gctgtgacgc
     2701 gaacgtctcg ctgcgccccc gcggcagcgg ccggctcggc acccgcaccg agacgaagaa
     2761 cgtcaattcg ctgcgctcca tcgaacgggc cgtgcggtac gagatccgga ggcaggcggc
     2821 ggtgctcgaa gccggcggcc tcgtcacgca agagacgaga cactggcacg aagacacggg
     2881 tacgacgagc gccggccggc ccaagtcgga tgccgacgac taccgatact ttcccgagcc
     2941 cgacctcgtg ccggtcgagc cgagtcgaac ctgggtggag gagctgcgcc agacgctgcc
     3001 cgaactgccc gccgtgcgcc gccgccggct cctcgccgag tggggctgca cggcactcga
     3061 attccaagac gtcgccaatt ccgacctggt ggacgaagtg gaagagaccg tcgccgcggg
     3121 ggcccggccg gatgcggccc gcaaatggtg gacgggcgag atcgcacgcc tcgcgaacca
     3181 gcaggatgcc gcccccgcct cgctcatcac cccgcggcag gttgccgact tggtgggcct
     3241 tgtcgattcg ggcaccctca ccgaccgact cgcccggcag gtgcttgccg gcgtcgtcgc
     3301 gggcgaaggc agcccggccg acgttgttga gaaacgcggc ctcgccgtcg tctccgacga
     3361 tggcgccctg gccgcggccg tcgacacggc cctcgcggcc cagcccgacg tcttggacaa
     3421 gatccgcggc ggcaaggtgc aggcagccgg cgcgatcatc ggcgcggtga tgaaggcgat
     3481 gaaggggcag gccgacgccg cccgggtgcg agagctggtt ctcgagcgcg cccgcgagtg
     3541 acgggcgccg ccctggcccg gagacgggca tctctttaaa ctggagtcat gttcatcgaa
     3601 ggaactcttc ggtggaaagt gaccgaagac tgtccatggg atctgctggt ggcgttgtgc
     3661 ctgcgcgata tggccgggct cgccgacctc ggcgagccgg tgttgcccgc cgtgacaccc
     3721 gcggtgcccg cggtcgccca caccgtgctc cggccgggtc gaacgggcgg cacggggccg
     3781 ggcagcgccg acaaggacgg gggcggaggg caccgaggtg ccttgcgtgc acagtggctg
     3841 gcctggtggg cgcgcacggc gacgcccggc acgaggccgt acgtgagcga cctcaggccg
     3901 ccacatttcg ccgcgttcga ccggcagctg gaactgcaag atctcatcga gacccattac
     3961 gtggcggcgg cgcgctgggc cgaccggcga cgtgaggagt acctgcgcac ccgcagcgag
     4021 cgtcgtgcgg cgttcgctgc cgacgtcgtc gagcccgtcc gtgagcggga gcatgcgctg
     4081 cgtcggcaag cgggctattt ccgcctcgac ctgtgcgtgc tgccgctcgc cgagcggggc
     4141 acctgggtca tcgcgcccga cacgatagcg atcagccgcg gcctgcgtga agacctcccg
     4201 gcgtttcgcg cctggtggcg gcggctcgcg gaggtgctcg tctagcgctc aaggcccggc
     4261 agggcccgga tcgacacggg aggcacaagg ccccatgggg agaacggacg gcaccggccg
     4321 cgtcgtcacg acgggccccg tcgacgacgc ggcgacgacc attctgcatg ccgacatgga
     4381 tgccttcttc gcctccgtcg aactgctcga gcgcccctgg ctgcggggca ggcccgccgt
     4441 cgtcggccgc cgcacgggca ggtcggtggt ctccagcgcg acctatgagg cacgccggtg
     4501 tggcgtgcac agcgccatgc cgatggctca ggcgttgcgg ctctgcccgc aggcggtcgt
     4561 gctggagcca cacatggaga agtaccgcgc cgtctcggcc cgggtcatgg cgatctttca
     4621 cggggtgacc ccgctcgtcg agcaggtcag cgtcgatgag gcgttcctcg acgtcgccgg
     4681 cgcccgccgc ctcttcggta caccttcgga ggttgcacgg ctcctacggg cccgggtgcg
     4741 tggcgaaacg gggctgtcct gttccatcgg cgcctctgcc gtcaaattcg tcgccaagct
     4801 tgcctcggcc aaggccaaac ccgacggcat gctcgtcgtg ccggccgggc agacccgcga
     4861 gttcctgcgg ccgctgccgg cgcgcgccct cgggggagtc gggcccgcga cgacgcagct
     4921 cctcgcagac cggggcatcc atacggtcgg cgacatcgct gcgacccccg aggcgacgct
     4981 cgtgcgctgg ctgggggagg ccggtggaac gaggctcgcg cgcctcgccc gtggcgtcga
     5041 cccccgcggc gtcgtaccgg gacgcaccga gaagtcgatc gggcgggaac agacgttcga
     5101 acacgacgtg accgacccga aggttctgcg acgcgaagtc ctccgcctcg ccctcgacac
     5161 cgcctcccgg ttgcgcaggg tgggtcttgt gggccgcacc gtgagcctca agattcggtt
     5221 cgccgacttc acgacgctca ctcgttcccg aacactgccc gaacccaccg acgtcggtcg
     5281 gcggatcggt caagaagcgt tgcagatcgt cgcggccgcc ggtgccgccc gcccggtgcg
     5341 cctcatcggg gtgcgcgtcg agaacctggc ggggccgagc gacggcgccc tcctgtggga
     5401 cccggacgac gattggcgag gtgtggagag cgtgaccgat accatcggtg cccgcttcgg
     5461 ccccgaggcc gtgacctcgg cctccctcct cggaacgcgt ccccgccgcg tcggcgacac
     5521 ctccagcgtc tctcgtgccg ccggagggtg agggcgctgc gggctatagt gagggaaaca
     5581 cgggagtccg gtgagccggg ctgagaggaa gcttctccaa gcttcgaccg tcgaacctga
     5641 tctgggtcat gccagcgcag ggaggtctga gaactctgcg gcagaagccg cccgtgccct
     5701 cgcaacgaaa gggcacgaaa catgacgaac gacacagacg agggtgccgc gcaccccgcc
     5761 gggcccgagg cagccggccc ggcgtcctcc gacgcggggg acgattcgac gagaggggcc
     5821 gtcttcgacc ctctaggcgg ggcggcccgc gccgcgcacc ccatccgccg cgcccggcga
     5881 tggcgttccc tcgacatcgt cgtcgcggcg gtgctgggcg tcgcctccgg cgtgatcttc
     5941 tggatctggg ggctcgcatg ggcgccggtg agcgcgattt tcgctctcac ccccggcctc
     6001 gacggcctga ctgcgggcgg atggctcttc gccggggtgt tgggcatgct cgtcatccgc
     6061 aaaccgggcg cggcggtctt caccgagctc gtggccggct tcgtcgaaat ggcgatcggc
     6121 aactcgtggg gtgccagcag catcgtctgg gcgctcgtgg agggcggggc ggcagagatc
     6181 gtgttcctga tcgtcggcta ccggcactgg cgggtctggg tcgcctccct ggcaggtgcg
     6241 gcggccggcg tcggcaccgc gttcctcgac acgactttca gcagcctcgc gggcctgtcc
     6301 gctccggcga agctcctata cggcgggtgc gcggtgctct ccggcctggt cgtcgccggt
     6361 atcctcacct ggcttctcgt gcgggcgctg gcccgtaccg gcgtcttgcg caggttcccc
     6421 tcggggcggg gcgctcgcat cgtcgatgcc gccgggccgg cggcaggctg agcggccgtc
     6481 atggcgctgg gccgcaccgg cggcgcacgg gtcgttgcgc gtggctgggg gctgcgatac
     6541 tccggccgca ggtccccggc gctcgtcggc atcgatctcg acatcgcggc cggccagcgg
     6601 gtgctccttg ccggcgcgag cggcgccggc aagagcaccc tactgcaggc gctggccggc
     6661 gtgatcggcg acgaggaaga cggcgagcag ttcggccttc tcaccgtgaa cggccggccg
     6721 tgcaccgaag cgccgggcgt cgcgggcctc gtcttgcaag acccggaatc gcagtcggtt
     6781 ctgccgaaga tcggcgacga cgtcgcattt ggctgcgaga acatggggat cgagcgcgag
     6841 gagatctggc gtcgggtagg cgagagcctt gccctcgtcg gcctcgacct gccctggcta
     6901 agcgcgaccg gcaccctctc cggtgggcag aaacagcgcc tcgcgctcgc cggcgtcctc
     6961 gccatgcggc ctggcctctt gcttctcgac gagccgaccg cgaacatcga cccggcgggc
     7021 gcccgccggg tccgtgatgc ggtcgcccac gcggccgcgg tgacgggggc gacccttctc
     7081 gtggtcgagc atcggatcga cctgtggctg ccggtcgtcg accggatgat cgtcttgggg
     7141 gaggaaggcg gcgttcgggc cgacggttct cccgcagacg tgctgcggcg ggacggcgac
     7201 gccctcgccg cagccggcgt ctgggtgccg ggggccctgt cgggttcctc cgagcccgcg
     7261 gcccgccgcc cgagtgcggc cgccttaccc ggcgcggccg cgggccgggc ccgcgaggcc
     7321 cctgcgacag ggccgacggg cggggcacct gcgggcgaac cgttgctttc cacgcacgac
     7381 ctcgccgtcg cgcccggcac gcgtgccgaa gtcgtcgccc gcccgcccgc cctcgccgtt
     7441 cgcgccggcg acgcgctcgc cgtgaccggt ccgaacggtt cgggaaagac gacccttgcc
     7501 ttgaccttgg gcggcctctt gcggccggcg gccggcgcgg tgcgggccgg cgaggccctc
     7561 gcggcggggc tggcgccaga cccctaccgg tggcgttcgc gccagctggc gacacgcatc
     7621 ggctccgtct ttcagcagcc ggagcggcag tttctcgccg gcacggtacg gggcgagttc
     7681 cgcatcggac tcgacgcggc gcgagtgccg cggaagcggg cggagcggat caccgaggcc
     7741 cttctcgaca ggctgggcct cacgcgcctg gccgaggcga acccttacac cctctcgggt
     7801 ggccagaagc ggcgtctgtc cgtggccgcg acgctcgcac tctccccgcc gcttctgatc
     7861 ctcgacgagc cgaccttcgg ccaagacctg cgcacctggc gcggactcgt cgaccttttg
     7921 gccgaacggc tcgaggccgg ccacggcatc gtcctcatca cccacgacga gcccctcgtc
     7981 gcggccctcg cgacccgaca cgtgagcctg cccgcgcacg agggtgtcgg ccgcccggct
     8041 cccgtgcccg ccggcccggg aggctcgcgg cagaaccggc aggccggctc gtgacgacgg
     8101 ccacgccgcc tggcacctcc gacgggcagc tgacggcggc acggctccat tccccggatg
     8161 cgacgtggcc ggccaggcgg ggcgtcgacc cgtttgcagg tctcgtcgcc gcgctcatcc
     8221 tcgggctcag cctcgtcgtg acgctcgaca tcgcctctgc cgccaccgcc ctggtgctgg
     8281 agcttctgtt tctgccgtgg gtgggggcgt cgggtcgggc gtggctacgc tgcacgtttc
     8341 tcctcgcggc agcttgcctc gccgccctca ccaccgcgct ctacggcgag gttgccggcc
     8401 acatctacgt gcgctggggc atcctcaccg tctcgagcgg ctccctcacc ctcgccgcgg
     8461 cgatcgcctt gcgcgtcttg gcggtggggg tacccgcggt cgttctgctg gcgagggtcg
     8521 acgtgacccg cctggccgac gccctggccc agatcgtgca ccttccggca cgtttcgtct
     8581 tgggggcgct ggccggcatg cggctggccg ccctcttcgt cgacgattgg cgcaggctct
     8641 ccctcacgcg ccgtgcccgc ggcctcggtg gcggcgggcc ggtgcgacgg gtgcggcgcg
     8701 ccgcatggca ggtgttcgcg ctgctcgtgg ccgcgatccg gcgcgggacg gtgctcgccg
     8761 tcgcgatgga ggcgcggggc ttcggccgtg cagccgggcg cacctgggcg aggcccgcca
     8821 ggctgactgc gcgcgacgtg accgtcatca ctctcgccgc ggcgatcgcg gcggcggcga
     8881 tcgtcgtgtc gctcgtcacg ggaagctgga actttgtgct cgcctgaggt gtcggtgccg
     8941 ggcgtcgtcg acgacctgct ccgcgacgcc cttcgtcgcc gccggccaga cggcgccccc
     9001 gcgaccgtcc tcatcgacgg gccggccggt gccggcaaga cgagcctcgc cgcggcgatc
     9061 gcccgccggt ggccggcgta cctctccgcg gccgtgccgg cctcgccgcc gctcgtgctc
     9121 ctccatatgg gcgacctcta ccccggctgg gagggcctgg cagagggtag ccgaaccctc
     9181 gccgagcgcg tcttgctgcc gcgctcgcac ggccgcgacg gccggttcca gacgtacgac
     9241 tgggtggccg cgcggcccgg cccatggcgc acggtgccgg cgtcggcgcc gcttctggtc
     9301 gaggggtgtg gggccttgag cagagcggcg gcggcgctcg cgacgctgcg cgtctggctc
     9361 gatgccgacg aagagctgcg gtggcggcgc gtctcgcggc gagacggcgg ggcattcgac
     9421 gacgaatggc cgcagtggag tcgtcagatg gtgagcttca tcacccgcga acagccgcgc
     9481 cggcacgccg acctgaccct ccgcagcgac gagcagggtg ttataaggcc gcgatgagcg
     9541 gtggcgtgcc tggccgtcac cgtcacgccc agccaagttc gtgcaaccga tcatcgtcca
     9601 gcccgtgaaa gtggccgatc tcgtgtacca gcgtgatgcg aacctcgcga cgcacgccgt
     9661 cgaggtcgcc gccggcccgc aagagcggca gtcggaacag caggattcgg tcgggcaagt
     9721 cgccgaaccc gtagtcgcct cgcctggtta gggcgacccc ctcgtagagg cccaagaggt
     9781 c
//
LOCUS       DASTNL010000214         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_715556, whole genome shotgun sequence.
ACCESSION   DASTNL010000214 DASTNL010000000
VERSION     DASTNL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715556"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFQ96_06900"
     CDS             complement(<1..366)
                     /locus_tag="VFQ96_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HEU0257549.1"
                     /translation="MSSGTNQPSKKERREAAREKARLMREAEKRRAKRRRWLVQGSVG
                     LGVLVVLAIIAVVVVTTVRPAGPGPRNMASGGILFTSSTKAALSPAQASGAATTPTKQ
                     DDSDGKAHITVYIDYQCPYC"
     gene            535..1947
                     /locus_tag="VFQ96_06905"
     CDS             535..1947
                     /locus_tag="VFQ96_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0257550.1"
                     /translation="MQTVLLFVVGVLVALAGLIVSIALHEMGHLVPAKLFRVTVTQYM
                     IGFGKTIFSRRHGETEYGVKWLPLGGYCAMVGMFPPEEGEGPGRDASTGFMQTMVQEG
                     SATGRAGHGGQAAVAAVAAQGLATGRPASAAASPAAGGERHPFYLLAPWKRIIVMLGG
                     PVMNLVIAVALFAIMFCGIGVQQYTTTVSAVSQCLKPATDTSQTCSAADLPSPAAAAG
                     LRAGDRIESVDGVAVTGWDQLTRLIEARAGQSVPVVVARNGRDVTLSVTPEASQNYVT
                     NAQGDIVTGASGKPETHTVGMIGISPSTALVRQSFGAALNATGQTVSGVVGVVVHLPQ
                     RLVGVWQAAFGPEKRAADSPVGMIGIGRMAGDIAASPVLPFVAKVQTMLSLLASLNVA
                     LFIFNLVPLLPLDGGHVAGALWDEVRRGIAKLAGRRYPGPVDITRLMPLTMAVIVVLG
                     GMSLLLAYADIVKPVTLLGG"
     gene            2062..>3765
                     /locus_tag="VFQ96_06910"
     CDS             2062..>3765
                     /locus_tag="VFQ96_06910"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU0257551.1"
                     /translation="MPTRLSTFFLRTLRDDPADAEVTSHKLLVRAGYIRRQAAGVFAW
                     LPLGLKVKAKIEAIIRQEMARAGAQEVHFPALMPREPYQVTGRWDEYGDLLFRLHDRK
                     GGDYLLAPTHEEAFTLLVKGLFSSYKDLPVTLYQIQDKYRDEARPRAGLLRGREFTMK
                     DAYSFDYTDAGLDVSYRAQRDAYERVFTRLGLDYVIVKADAGAMGGSRSEEFLHPTPV
                     GEDTFVRSEGGYAANVEAYVATPPAPQAVERPTDDLARPVVVDTPDAGTIDALVEVAN
                     GVRPRPGGTPWQATDMLKNVVLALVDGDGGREVVAIGLPGDREVDLKRAEVAFAPRAV
                     EPASAEDLAAAGLVAGYIGPWSRTGSALGRGSASGVRYLLDPRVVPGSLWVSGANEEG
                     RHAFGLVAGRDFTADGVADVAEVRDGDPAPDGSGPVRLSRGTEIGHVFQLGRKYAEAL
                     GLQVLDENGKLVTVTMGSYGIGVTRVLAILAERNSDDKGLAWPESVAPFDVHVVATGR
                     DGAVYETAEAVSAELEEAGLDVLYDDRRKVSPGVKFGDAELLGVPHIVVVGRRAAEGI
                     VE"
BASE COUNT          536 a         1289 c         1329 g          611 t
ORIGIN      
        1 gcagtacggg cactgatagt cgatgtagac ggtgatgtgg gccttgccgt cggagtcatc
       61 ttgcttggtc ggggtcgtcg cggcgccgga ggcctgggcg ggggagaggg ccgccttcgt
      121 gctcgaggtg aagaggatgc ctcctgaagc catgttcctc ggccccggcc cggccgggcg
      181 caccgtggtc acgactacga cggcgatgat cgccagtacg acgagtacac ccaggccgac
      241 ggaaccctgc acgagccaac gccgtctttt cgcgcgacgc ttctccgcct cccgcatgag
      301 ccgcgccttc tcgcgcgcgg cctcgcgacg ttctttcttc gacggttggt tcgtgccgga
      361 cgacatcgcg ctccttcgta gtgcaccgcg ttcctcgtcg aggcgacggg agagcggcga
      421 cagccttcat tatgacggag ggcggccagg gtcacggcgc tcctcgccgc accggcggcg
      481 cgtgcatagc ggccagacag cccggccaca gtcgccccgc attaccctcg aggtatgcaa
      541 acggtgctgc tcttcgtggt cggggtgctc gtcgccctcg cgggcctcat cgtctccatc
      601 gccctgcacg agatggggca ccttgtgccg gccaagctct tccgcgtcac cgtcacgcag
      661 tacatgatcg gcttcggcaa gaccatcttc tcccgtcgtc acggggagac cgaatatggc
      721 gtcaagtggc tgccgttggg tgggtattgc gcgatggtcg gcatgttccc gccggaagaa
      781 ggcgaggggc cgggccgaga cgcgagcacc gggttcatgc agaccatggt gcaagagggc
      841 tccgccacgg gcagggccgg gcacggcggc caggcggcgg tggcggctgt ggcggcgcag
      901 ggcctggcca cgggaaggcc ggcgtcggcg gcggcctcgc ccgcggccgg cggcgagcgg
      961 catcccttct accttctggc tccctggaag cggatcatcg tcatgctcgg cgggccggtc
     1021 atgaatctcg tgatcgcggt cgcgctcttc gccatcatgt tctgcggcat cggggtgcag
     1081 cagtacacga cgacggtctc cgcggtctcc caatgtttga agccggcgac cgacacgagc
     1141 cagacgtgct ccgcggccga tctgccgagc cccgccgcgg cggccgggct gcgagcgggc
     1201 gatcggattg aatctgtcga cggcgtcgcc gtgaccggct gggatcagct gacgaggctc
     1261 atcgaggccc gtgccggtca gagcgtgccg gtcgtcgtcg cccggaacgg ccgcgacgtg
     1321 acgctctcgg tcacgccgga ggcctcgcag aactatgtca cgaacgctca gggagatatc
     1381 gtcaccggcg cctccggcaa gccggagacg cataccgtcg gcatgatcgg gatcagcccc
     1441 agcaccgcgc tcgtgcggca gtcgttcggg gctgcgttga acgccaccgg ccagacggtc
     1501 tccggcgtcg tcggcgtcgt cgtgcatctt ccgcaacgcc tcgtcggcgt ctggcaggcc
     1561 gcgttcggtc cggagaagcg cgccgccgac tcgcccgtcg gcatgatcgg catcggccgc
     1621 atggccggcg acatcgcggc ctcccctgtg ctgccgttcg tggcgaaggt gcagacgatg
     1681 ctctcgctcc tcgcgtcgct gaacgtggcg ttgttcatct tcaacctggt gccccttctg
     1741 ccgctcgacg ggggtcatgt cgcgggcgcc ctgtgggacg aggtgcggcg cggcatcgcg
     1801 aagctcgcag ggcgcaggta cccgggcccg gtcgatatca cgcggctcat gccgttgacc
     1861 atggcggtca tcgtcgtctt gggcggcatg agcctgctcc tcgcctacgc cgacatcgtg
     1921 aagccggtca ccctcctcgg cgggtgagtc ggggcggggc cggggctggc accgcgggaa
     1981 cccgtgccga gcatcgtcgc ctccttcctg cgccgtcggg cgcgggcgag gccgccgcct
     2041 cgccccagta aggtggaggg cgtgccgaca cgcctctcga ctttcttttt gcgcaccctt
     2101 cgtgacgacc ctgccgacgc ggaggtcacg agccacaagc tcctggtgcg cgccggctac
     2161 atccgccgcc aggccgccgg cgtcttcgcc tggctgccgc tcggtctcaa ggtgaaggcc
     2221 aagatcgagg cgatcatccg ccaagagatg gcgcgggccg gcgcccagga ggtgcatttt
     2281 cccgcgctga tgccgcgcga gccgtatcaa gtcacagggc gttgggatga gtacggcgac
     2341 ctgctcttcc ggctgcacga tcgcaagggt ggggactacc tgctcgcccc gacccacgag
     2401 gaggcgttta cactgctcgt gaaagggctg ttctcctcgt acaaggacct ccccgtcacg
     2461 ctctaccaga ttcaagacaa gtatcgagac gaggccaggc cgcgtgccgg gcttttgcgt
     2521 gggcgcgagt tcaccatgaa agacgcgtat tcgttcgact acacggatgc cggtctcgac
     2581 gtctcctacc gtgcgcaacg cgacgcatac gagagggtct tcacccggct cgggctcgac
     2641 tacgtgatcg tgaaggccga cgcgggcgcg atgggcggct cgcgttccga agagttcctg
     2701 catccgacgc cggtcggcga agacaccttc gtgcgttccg aaggcggcta tgcggccaac
     2761 gtcgaggcct acgtcgcgac cccgccggca ccgcaggccg tcgaacgccc cacagacgac
     2821 ctcgcccgcc cggtcgtcgt cgacactccc gacgccggca cgatcgacgc gctcgtcgag
     2881 gtcgcgaacg gggtgcgccc gcgcccgggc ggcacgccgt ggcaggccac cgacatgctg
     2941 aagaacgtcg tgctcgccct cgtggacggc gatggcggtc gcgaggtcgt ggcgatcggc
     3001 cttcccggcg accgggaggt cgacctgaag cgcgccgagg tggccttcgc cccgcgcgcg
     3061 gtcgagccgg cgagcgccga agacctcgcc gcggcgggcc tcgtcgccgg ctacatcggg
     3121 ccctggtcgc gaacgggttc ggcactcggg cgcggttcgg cctccggcgt gcgctacctt
     3181 ctcgaccccc gcgtcgtgcc gggcagcctc tgggtctccg gcgcgaacga agaaggccga
     3241 cacgccttcg gcctggtcgc cggccgggat ttcaccgccg acggcgtggc cgacgtggca
     3301 gaggtgcgag acggcgaccc ggcgcccgac ggctcggggc ccgtacgact ctcgcgcggc
     3361 acggagatcg ggcacgtctt tcagctgggg cgaaaatatg cggaggcgct cgggcttcag
     3421 gtgctcgacg agaacggcaa gctcgtcacc gtaaccatgg gctcctacgg catcggcgtc
     3481 acccgggtgc tggcgatcct cgcggagcgc aacagcgacg acaagggcct cgcctggccg
     3541 gagtcggttg cgccgttcga cgtgcacgtc gtcgcgacag gtcgggatgg tgccgtctac
     3601 gagacggcgg aggcggtctc ggccgaactg gaggaggcgg ggctcgacgt gctgtacgac
     3661 gaccgccgca aggtgtcccc cggggtgaag ttcggcgatg ccgaactgct cggcgtaccc
     3721 cacatcgtcg tcgtcgggcg tcgcgccgcc gaagggatcg tcgaa
//
LOCUS       DASTNL010000215         5389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_717802, whole genome shotgun sequence.
ACCESSION   DASTNL010000215 DASTNL010000000
VERSION     DASTNL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717802"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VFQ96_06915"
     CDS             <1..1833
                     /locus_tag="VFQ96_06915"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEU0257552.1"
                     /translation="IPRARLGIVPIALTALSYLVLLAVAGHPRLHRVRQGRWVPVAAA
                     AGAVALAATIGLTAPGFTAHGVRADALSRLQAATTGSSVSSLIDLGKDLHRPAAVPVL
                     DYTTTARGGLYLTLTTLGTFTGDTWTRTAQSIRPLGPGGEVGQVPGLSNAVPAREVRT
                     TVGLDDLHSPWAPAPYPATRVSGLAGRWDVGTSDLTIGADGSSVAGQRYTVDSKELSP
                     TPAQLAASSTIYPAAARADLALPRSIPKNITAAAKKATRGAHSNYQRAVDLQNYFRDG
                     DFTYSLQTPLRQGYNGDGLAVISTFLRVKSGYCVHFASAMAVMARILGIPSRIAIGYL
                     PGVPLTTNSGKTVYTVTSDELHSWPELYFDGIGWVRFEPTVSLGSAPSYTETGSQPTK
                     ASSEESSASASTPASSSPSPSQAPATGKAVGTSAHAGTQAPAVPLPGFLLAVLVLLLL
                     PALVARLRRRGRRMLVRLGRRPAESAWSLARSAARDLRLAAPARDETVRATIARLPAP
                     AVTPALLRLRDAVERERYAGGVGHGADRALLAADLRAACRALCGYAGRGRRLAALFAP
                     ASLVSERRGGLPPSATPSRAGSDLVAYNPGREHQPSRPVHARRP"
     gene            1877..2344
                     /locus_tag="VFQ96_06920"
     CDS             1877..2344
                     /locus_tag="VFQ96_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEU0257553.1"
                     /translation="MPTLDGLGEGLARVAAGTVIRTEVTLESLHDGILVSAAVEARAV
                     GECSRCLKEIELPVEVDIQELFAYSSDEAYEYEVRGGEIDCGPLVHDTVVLALPFQPV
                     CRPECPGLDPATGMLRTSHADAEPARHDPRWAALAGLRVVNESGADEPARDEE"
     gene            2350..2553
                     /gene="rpmF"
                     /locus_tag="VFQ96_06925"
     CDS             2350..2553
                     /gene="rpmF"
                     /locus_tag="VFQ96_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU0257554.1"
                     /translation="MAVPKRKMSRASTRMRRAQWKAEVPTLVKTVENGRVSYSLPHHA
                     RVVEDSAGTPLFLEYKGRRVADV"
     gene            2567..3298
                     /gene="rnc"
                     /locus_tag="VFQ96_06930"
     CDS             2567..3298
                     /gene="rnc"
                     /locus_tag="VFQ96_06930"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU0257555.1"
                     /translation="MRHAATGASADLDALRNALGVSLHDEILTLALTHRSYAYEHGGV
                     AHNERLEFLGDSILGQAVTVRLFTDNPDLPEGELAKRRASLVSSVALAEIAGTIGLGR
                     YIRLGRGEDTSGGRHKASILADTVEAIIGATYLDAGDDVATDLVLRLVTPLMRDPARF
                     GAAMDPKTSLQERVAQLGGEAPEYEVVGTGPDHDRTFHATVTVPGVVVGRGEGSSKKH
                     AEMAAALEAFALLNDAAGGSARDDA"
     gene            3291..4205
                     /gene="mutM"
                     /locus_tag="VFQ96_06935"
     CDS             3291..4205
                     /gene="mutM"
                     /locus_tag="VFQ96_06935"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU0257556.1"
                     /translation="MPELPEVEAMRRGLAPLAGSVIRDVEVLDPRGISRHDRSDGDFR
                     QLLLGRLIAGSARRGKFLWLPLADDEARPEAAGATALLAHMGMSGHVMVLPPGAGRER
                     ALRVRILLDDRAGRAQALHFVDQRTFGSLAVDRLIPTADAHPAGAGAQAPLLPRQVAH
                     IARDPLDPFFDERLFFARLTRRNSTIKRALLDQGLVSGVGNIYADETLWAARTHFNQP
                     TLGMGLVRARRILARLREILAAAREDSGTSFDTQYVDVNGRSGRYGQHLAVYGREGRP
                     CRRCGTPIRRVPFMNRSSHFCPRCQRIR"
     gene            4312..>5389
                     /locus_tag="VFQ96_06940"
     CDS             4312..>5389
                     /locus_tag="VFQ96_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0257557.1"
                     /translation="MYLKSLTVKGFKSFARPATFLFEPGVTCVVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGSMQDVIFAGTPQRPPLGRAEVVLTIDNGDGALPIDYAEVTISR
                     TMFRGGGSEYAVNGERCRLVDVQELLSDSGLGRHMHVIVGQGRLDAILGASAEERRAL
                     VEEAAGILKHRRRKESTLRKLEAMQANLDRVNDLVAEIGRQLKPLGRQARAAEEARSV
                     QATVRDATARLLADDVAVLKAQIEADETEERRAQGEAAVLREELDTARSAAGRADSAF
                     DAGLVEASRGVLYDLKQAGDRLAGLDALTRQRLVLLEDGETPATVRVVDDEEVRAAAA
                     HTASLASEGAEAEAALEAARAGEER"
BASE COUNT          785 a         1946 c         1897 g          761 t
ORIGIN      
        1 atcccgcgcg cgaggctcgg catagtcccg atcgcgctca ccgccctcag ctacctcgtg
       61 ctgctggccg tcgccgggca cccgcggctc catcgggtgc gccaagggcg gtgggtcccc
      121 gtggccgctg cggcaggcgc cgtcgcgctc gccgcgacga tcggcttgac cgcgcccggc
      181 ttcacggcgc acggcgtgcg ggccgacgcg ctctcccgcc tccaggcggc cacaacgggc
      241 agcagcgtca gctcgctcat cgacctcggc aaagacctgc acagacccgc cgcagtgcct
      301 gttctcgact acacgacgac ggcgcgtggc ggcctgtacc tgaccctcac gacgctcggc
      361 acgttcacgg gcgacacctg gacgcgcacg gcgcaaagca tccgccccct cggcccgggc
      421 ggcgaggtgg ggcaggtgcc cgggctctcg aatgcggtgc ccgcgagaga ggtgcgcacg
      481 acggtgggcc tcgacgacct gcacagcccg tgggcaccgg cgccgtaccc ggccacgcgc
      541 gtcagcggcc ttgcgggcag gtgggatgtc ggcacgtcgg atctcacgat cggcgccgac
      601 gggtcgagcg tcgccggcca acgctacacg gtcgacagca aagagctgag tccgacgccg
      661 gcgcaactcg cggcctcctc cacgatctac ccggccgccg cccgggccga cctcgccttg
      721 ccgcgttcca ttccgaagaa catcacggcg gcggccaaga aggcgacccg aggcgcgcac
      781 agcaactacc aacgagccgt ggatctgcaa aactacttcc gcgacggcga cttcacgtat
      841 tctctgcaga cgccgctgcg gcagggctac aacggcgacg gcctcgcggt gatctccaca
      901 tttctgcggg tgaagagcgg ctattgcgtg cacttcgcct cggccatggc cgtcatggcg
      961 cgcatcctcg gcatcccgtc gcggatcgcc atcggctacc tgccgggcgt gcctctcacc
     1021 acgaactctg gcaagaccgt ctacacggtc acctccgacg agctccactc gtggccggaa
     1081 ctctacttcg acggcatcgg atgggtgcgg ttcgagccga ccgtgagcct gggctccgcg
     1141 ccgtcgtaca ccgagacggg aagccaaccg accaaggcct cctccgagga gagctccgct
     1201 tctgcgtcca cgccggcctc ctcctcgccc tcgccgagcc aggcaccggc cacgggcaag
     1261 gcggtcggga cctcggccca tgccggcacc caggcgccgg ccgtgccgct gcccggcttc
     1321 ctcctggccg tcctcgtgct cctgctgctg cccgcgctcg tcgcccggct gcgccggagg
     1381 gggcgccgga tgctcgtgcg gcttggcaga cggccggcgg agtcggcctg gtccctggcg
     1441 cgctctgccg cccgcgacct gcgcctcgcg gcgcccgcgc gggacgagac cgtgcgtgcg
     1501 acgatcgccc gtctgcccgc gcccgccgtg acgccggctc tcctgcggct gcgtgacgcg
     1561 gttgaacggg aacgctacgc cggcggggtg gggcacggcg ccgaccgggc ccttctggcc
     1621 gccgacctgc gggcggcatg tcgggccttg tgcgggtacg cgggccgcgg gcggcgcctc
     1681 gcggcgctct tcgcccccgc ctctctcgtg tcggagcgac ggggcggcct gccgcccagc
     1741 gccacgccgt cccgtgccgg gagcgacctc gtcgcgtaca atccaggccg tgagcaccag
     1801 ccatcgcggc ccgtacacgc tcgacgtccg tgaccttcga cgccatcccg ggcagagcga
     1861 acaccacagt cttgtcgtgc ctaccttaga cgggctcggc gaggggctcg cccgggtggc
     1921 ggccggcacc gtcatccgca ccgaggtgac gctcgagtcg ctgcacgacg gtatcctcgt
     1981 cagcgccgcc gtcgaggccc gggccgtggg tgaatgctcc cggtgtctga aggagatcga
     2041 gctgcccgtc gaagtcgata ttcaggagct tttcgcgtat tcttctgacg aggcctatga
     2101 gtacgaggtc cgcggcggcg agattgactg cgggcctctt gttcatgaca ccgtggttct
     2161 ggcgttgcct ttccagccgg tttgccggcc agaatgtccc gggctcgacc cggcgacggg
     2221 gatgctccgg acgagccacg ccgatgcaga gcccgcgcgg cacgatccgc ggtgggcggc
     2281 gctggcgggc ttgcgggtgg tcaacgagag cggcgccgac gagcccgcac gagacgagga
     2341 gtaagagcca tggcggtacc caagcgcaag atgtcccggg ccagcacgcg gatgcgtcgg
     2401 gcccagtgga aggccgaagt tccgaccctg gtcaagaccg tggagaacgg ccgcgtcagc
     2461 tacagcctgc cgcaccatgc ccgcgtggtc gaagactccg ccggcacgcc gctgttcctt
     2521 gaatacaagg gccgcagggt cgccgacgtc tgagcgtcgg cgccacatgc gccacgccgc
     2581 gacgggcgcc agcgccgacc tcgatgcgct acgcaatgcg ctcggcgtgt cgctgcatga
     2641 cgagattttg accctcgcgc tcacccatcg ctcctatgcc tacgagcatg gcggcgtcgc
     2701 gcataacgag cgtctcgagt tcttgggcga ctccatcctc ggccaggcgg tgacggtgcg
     2761 gctgttcacg gacaaccccg acttgcccga gggggagttg gcgaagcgcc gcgcaagcct
     2821 tgtcagttcc gtcgcgctcg ccgagatcgc cggcacgatc gggctggggc ggtacatccg
     2881 actgggccgc ggcgaagaca cgagcggcgg gcggcacaag gcttcgatcc ttgccgacac
     2941 ggtggaggcc atcatcgggg ccacctacct cgacgccggc gacgacgtgg cgaccgacct
     3001 cgtgctccga ctcgtgacac cgctcatgcg cgacccggcg cggttcggcg ccgccatgga
     3061 tccgaagacc agcctgcaag aacgcgtcgc ccagttgggc ggggaggcgc ccgaatacga
     3121 ggttgtgggc accggccccg accatgaccg caccttccac gccaccgtca ccgtgccggg
     3181 tgtcgtcgtc ggccgcggcg agggctccag caagaaacac gccgagatgg ccgccgcgtt
     3241 ggaggcgttc gccctcttga acgacgcggc cggcggctcg gcccgcgacg atgcctgagc
     3301 ttcccgaggt tgaggcgatg cgccgcgggc tcgcgccgct cgccgggtcg gtcattcgag
     3361 acgtcgaggt tctcgaccca cgcggcatca gccggcatga ccgctccgac ggcgactttc
     3421 gccagctgtt gctggggcgc ctcatcgcgg gcagcgcccg gcggggaaag ttcctgtggc
     3481 tgccgctggc cgacgacgaa gcgcgtcccg aggcggcggg ggcgacggcg ctcctcgccc
     3541 acatgggcat gagcggccac gtcatggtcc tgccacccgg ggccgggagg gaacgggcgc
     3601 tgcgcgtcag gatccttctc gacgaccggg cgggccgcgc gcaggcgctc catttcgtcg
     3661 accagcgcac cttcggctcg ctcgccgtcg accgcctcat cccgacggcc gacgcccacc
     3721 ctgcgggtgc cggcgcgcag gcgccccttc tgccccggca ggtcgcgcat atcgcccgtg
     3781 acccgctcga cccgttcttc gacgagcgcc tcttcttcgc aaggctcacc cgcaggaaca
     3841 gcacgatcaa acgggccctt ctcgaccagg gcctcgtgag cggggtcggc aacatctatg
     3901 cggacgagac gctgtgggcc gcacgcaccc acttcaacca gccgacgctc ggcatggggc
     3961 tcgttcgcgc gaggcgcatc ctcgcccgcc tgcgggagat tctcgccgcc gcccgagaag
     4021 actcgggcac gagcttcgat acccagtacg tcgacgtgaa cggcaggtcg ggccgatacg
     4081 gccagcacct ggccgtctac ggccgcgagg gtcgaccgtg tcggcgctgc ggcacgccga
     4141 tccgccgcgt gcccttcatg aacaggtcgt cgcacttctg cccgaggtgc cagcggatcc
     4201 gctgaacgtc ggcccgtttt cggcggcggg cgcggcgaga cgggccgccg tacgccgtcc
     4261 ggtaccgtgg atgccgagac gtgcggccgg gtcggcagaa ggagcgcggg tttgtacctg
     4321 aagagcctca ccgtgaaggg gttcaagtcg ttcgcgcgcc ccgcgacatt cctcttcgag
     4381 cccggcgtca cctgcgtcgt cggcccgaac gggtcgggca agtcgaacgt cgtcgacgcg
     4441 ctcgcctggg tcatggggga acagggcgcc aaatcgctgc gcggcggcag catgcaagac
     4501 gtgatcttcg ccggcacccc gcaacggccc cctctcggca gggccgaggt cgtgctcacc
     4561 atcgacaacg gcgacggggc gttgcccatc gactacgccg aggtgaccat cagccgaacg
     4621 atgttccgcg gcggcggcag cgaatatgcc gtgaacggcg agcgctgccg gctcgtcgac
     4681 gtgcaagagc tcttgagcga ttccgggctt ggccggcaca tgcacgtcat cgtcggccag
     4741 ggcaggctcg acgcgattct cggcgcgagc gcggaggagc ggcgcgccct cgtcgaggag
     4801 gccgccggca ttctgaagca tcgccgccgc aaagagagca cgctgcgcaa gctcgaggcg
     4861 atgcaggcga acctcgaccg cgtgaacgat ctcgtggcgg agatcggccg gcagctgaag
     4921 cctctggggc gccaggcacg tgccgccgaa gaggcccggt cggtgcaggc cacggtgcgc
     4981 gacgccacgg cccggcttct tgccgacgac gtggccgtgc tgaaggcgca gatcgaagcg
     5041 gacgagaccg aagagcgacg cgcgcagggt gaggcggcgg tgctgcgcga agagctcgac
     5101 accgcccggt cggctgccgg ccgcgcggat tccgccttcg acgcggggct tgtcgaggcc
     5161 agccgggggg tgttgtacga cctcaagcag gcgggcgacc ggctggccgg cctcgatgcg
     5221 ctcacgaggc agcgcctcgt cctgctggaa gacggcgaga cgcccgccac ggtgcgggtc
     5281 gtcgatgacg aggaggtgcg ggcggccgcc gcccacaccg caagccttgc ctcggaaggg
     5341 gcagaggcag aggcagcgct cgaggcggcg cgggcggggg aggagcgcg
//
LOCUS       DASTNL010000216         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_903159, whole genome shotgun sequence.
ACCESSION   DASTNL010000216 DASTNL010000000
VERSION     DASTNL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903159"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFQ96_06945"
     CDS             complement(<1..853)
                     /locus_tag="VFQ96_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257558.1"
                     /translation="MAERLAVLLSAGIAPAAAWRHVARSREAASAARAAMAGGDVGAA
                     LLLASREAGAAAQDAWAALAATWRVAVASGAPPATSLRALASSIRALGKVRRQAGTAL
                     AGPAATARMVLVMPAVAVVLGALLGFGTVATLVTTVPGFLCLALAVALILAAARWNRR
                     MVRRAAPREKAPGLALDLLAVAMSGGVSVDKARRLVSRSLPARLAASRAELGAAEDIL
                     RLASAAGAPAAELLRGEAAARRLAAVVTAERDAVRLSVRLMLPLGLCVLPAFMLVGVL
                     PLLFSILS"
     gene            complement(1026..2153)
                     /locus_tag="VFQ96_06950"
     CDS             complement(1026..2153)
                     /locus_tag="VFQ96_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HEU0257559.1"
                     /translation="MTPFVPMPRRAGPHAAGRLERAFLPPADPPRAASPGLVPARRRF
                     VPEPRGGPPSAVIGGRESAAPTGRERAAVDLGALTPYARDPTVTDLLVTGDGSLWSDS
                     AAGLVEIAHRRWNEEEARALAVRLVALGGRHIDEANPCVDVRLHDGIRVHAVLPPIST
                     TGAVLSVRLPGARRLSLADLCRDGTLDDETATMLRTAVEERRNLLITGATGSGKTTLL
                     AALLSEVGPAERLVVIEDVAELRIRHPHVVGLEARQPNLEGAGGVGLARLLREALRMR
                     PDRIVLGECRGAEIRELLTALNTGHDGGAGTLHANSLADVPSRLEALGALAGMSDQTV
                     ARQTVSALDLVVHVEREGARRRVAGTAHLALDDAGRLCVEP"
     gene            2336..>2731
                     /locus_tag="VFQ96_06955"
     CDS             2336..>2731
                     /locus_tag="VFQ96_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257560.1"
                     /translation="MSVSPAKSAAKASGGAGPAAPAAQFDDMRAGTALRFEAGDEVVA
                     AYETAEVLPALARVDAASRAGRWAYGFVAYDAAPAFDGALVTYASTLPAPQVASSSTA
                     AYDPGAAVYDPGPGTAAPRDQGPKGARPHP"
BASE COUNT          423 a         1044 c          972 g          292 t
ORIGIN      
        1 aggagaggat cgagaagagc agcggcagca cgccgaccag catgaaggcc ggcagcacac
       61 acaaccccaa gggcagcatc agccgcacgg agagccgcac ggcgtcacgc tcggccgtca
      121 cgacggcggc gagccgccgg gcagccgcct cgccgcggag gagttcggcc gcgggcgccc
      181 cggcggccga ggcgaggcgc aggatgtctt cggccgcgcc tagctccgcg cgcgaggccg
      241 ccaggcgggc gggcagactg cgcgacacca gacgacgggc cttgtcgacg gagaccccgc
      301 cgctcatcgc caccgcaagc aggtcgaggg cgagcccggg cgccttttcc cgcggggcgg
      361 cccgacgcac catacggcgg ttccagcggg cggcggcgag aatgagcgcc acggcaaggg
      421 ccaggcacaa gaacccggga acggtcgtca caagggtggc caccgtgccg aaaccgagca
      481 gggcgcccaa gacgacggcg acggccggca tcacgagaac catccgcgcg gtcgcggcag
      541 ggccggccaa ggcggtgccc gcctggcggc gcaccttgcc caaggcgcga atcgacgagg
      601 cgagcgcccg caggctcgtc gccggaggcg cacccgaggc gaccgccacc cgccaggtgg
      661 cggcgagcgc ggcccaggcg tcttgggccg cggcgccggc ctcgcgggag gcgagaagga
      721 gcgccgcacc cacgtcgccg cccgccatcg ccgcccgcgc cgccgacgcc gcctcgcggc
      781 tgcgcgcgac gtggcgccac gccgcggccg gggcgatgcc ggcggagaga agcactgcga
      841 gccgttccgc cacgccggcc acactgtctg attccggttc cgtgcccgcc ctcacggctc
      901 tgcgcccacg ttcacagccc ccacgccctt ccgcccctga acgccgtcgc ggacctacgc
      961 cgatggtcac gatccacacg cacgctcacg ccttacctcc acgctcatac ctcatatgcc
     1021 cacgcctacg gctccacaca cagccggccc gcgtcgtcga gggcgaggtg cgcggtgcct
     1081 gcgactcgcc gcctggcgcc ctcccgctcc acatggacga ccaggtcgag cgcgctgacg
     1141 gtctgccggg ccaccgtctg atcggacatg ccggccaacg cccccagcgc ctccagccgc
     1201 gacggcacgt cggccaagga attcgcgtgc agcgtgcccg ctcccccgtc gtggccggta
     1261 ttgagcgcgg tcaaaagctc acggatctcg gcgccgcggc actcaccgag gacgatccgg
     1321 tcggggcgca tgcgcaacgc ctcacgcagg agcctggcaa ggccgacgcc gccggcaccc
     1381 tcgaggttcg gctgccgggc ctccaggccc acgacatgcg gatggcggat acgcaactcg
     1441 gcgacgtctt cgatgacaac gagacgctcc gccgggccga cctcggaaag cagggcggcc
     1501 agcagggtcg tcttgccact gccggtcgcg ccggtgatga gcaggttgcg gcgctcttcg
     1561 acggcggtgc gcagcatcgt cgccgtctcg tcatcaagcg tgccgtcgcg gcacagatcc
     1621 gccaggctga ggcggcgagc gcccggcagg cgcaccgaca gcaccgcacc ggtcgtggag
     1681 atgggcggca gaaccgcgtg cacccggatg ccgtcatgca accgcacgtc gacgcagggg
     1741 ttggcctcgt cgatgtggcg gccgccgagg gcgaccagac gcaccgcgag cgctcgagcc
     1801 tcctcctcat tccatcggcg atgcgcgatc tctacgagcc cggcggcgct gtcagaccag
     1861 agcgacccgt cgcccgtcac cagcaggtcg gtgaccgtcg ggtctctcgc atagggcgtc
     1921 aacgccccga ggtcgacggc ggccctctcc cgccccgtgg gggcggcact ctctcgcccg
     1981 cctatgaccg cggacggcgg cccacctcga ggttccggca cgaaccgccg ccgggcgggc
     2041 acaaggcccg gcgaggcggc gcgtggcggg tcggccggcg gcaagaaagc gcgctccagg
     2101 cggccggcag cgtgcgggcc ggcacgcctc ggcatgggga cgaaaggtgt cacgggggcg
     2161 aggctaggcc gaaaatcggg cggccccgcc ccgacggcgt ccgcggtggg aacgagtacg
     2221 cgccattgtg ccggtggagg aacagccggc cgccccgcgt cacgaagctt gcgccgcaat
     2281 gtccgtaccc ggtaaaccgc acacacggaa agcgccgaca cactactgtc gtgatgtgtc
     2341 cgtctcgccg gcaaaatccg cggccaaggc ctcgggagga gccgggccgg ccgccccggc
     2401 ggcgcagttc gacgacatgc gggcgggcac ggcgttgcgt ttcgaggccg gcgacgaggt
     2461 cgtcgcggcg tacgagacgg cggaggtgtt gcccgcgctc gcacgcgtcg acgcggcgtc
     2521 tcgggcgggg cgttgggcgt acggattcgt cgcctacgac gcagctcccg cgttcgacgg
     2581 cgccctcgtc acctacgcct ccaccctgcc cgccccgcaa gtcgcgagct caagcaccgc
     2641 cgcgtacgac ccgggcgcgg ccgtgtacga cccgggcccg ggcaccgcag ccccgcgcga
     2701 ccaaggcccg aagggcgctc gaccacaccc a
//
LOCUS       DASTNL010000217         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_348459, whole genome shotgun sequence.
ACCESSION   DASTNL010000217 DASTNL010000000
VERSION     DASTNL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348459"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VFQ96_06960"
     CDS             complement(<1..1391)
                     /locus_tag="VFQ96_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257561.1"
                     /translation="MAEATTAARATAGSRPETHAATHPAAAAARDYARDAVTAFLLVA
                     SLFMPWVASAPHGQVATGIAAAHIDVLLVTMVTLVALAIPHLGRVPALGTAWSPDTVR
                     RVRLVIGLPYAVLVIVYLVLALVTQLTLGPALAFGMAGAILAVQPRQRELPPIPRAGR
                     VWLRGTVVVAGLAVVFTLVQIAEMFSIFSANGPAAFILSIVLGLAASGILAVAAVGAL
                     TGHRAWRRIGIGLGVAAAVLILLSLNPQLVLVDATLHGPAPTFSLMFWVAFGAAAAAP
                     ALGRRLPAVADAGAAWFAVVRVILGLAVSCGILVVIVSSIELSYEQLIGPFAPLAVTE
                     WMIALAVGVLLTAGGVTVWAIGRTKTRSAVEAISVFAGILFVLGLILTVVWSLPSALP
                     FGGAATLAPLIAFAFPAALLGCLWVPASSRARYASLPHGGRSRVSSAEQGRADDDVAR
                     TGATRYHDDATQS"
     gene            1555..2064
                     /locus_tag="VFQ96_06965"
     CDS             1555..2064
                     /locus_tag="VFQ96_06965"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0257562.1"
                     /translation="MSVHTVVESALGGITLVGGDEGLEGLYMAEHRHPRRDGFGQAAT
                     GRERHALFDAATAQLGEYFAGERTRFDLVLAPHGTPFQLTVWNLLRRIEYGQTWSYRR
                     LAEEYGNPKAVRAVAAANARNPLSIVVPCHRVIGSDGSLVGYGGGLWRKEFLLELEGG
                     RGTGQGTLF"
     gene            2061..2930
                     /locus_tag="VFQ96_06970"
     CDS             2061..2930
                     /locus_tag="VFQ96_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEU0257563.1"
                     /translation="MTGRALSRLAANGPFAAHAALAAMAVHAVPGLERVDVGAGLVER
                     MLCFSHGPGLVRMQVSDSGVTVEAPAAVLPEAAARVRRYFDLETDLGQVRAVLARDPL
                     LAPLVAHRPGLRVLGSFDPFESAACIVLGQQVSLAAARTLQSRLVAALGTPGCDGLSL
                     FPGPRRIAACPEPELRAAIGLTRSRARTLHGLAEACAAGLRLDAPLSEVRAALLNLWG
                     VGPWTVDFLSLRALGDRDACPAGDLVLRRALGMTTSRDVLARAAGWSPVRAYAVFHLW
                     TEIGYEAGDTPGR"
     gene            3356..3823
                     /locus_tag="VFQ96_06975"
     CDS             3356..3823
                     /locus_tag="VFQ96_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0257564.1"
                     /translation="MSVNPELAGRVLPPRGPYLVGREKVREFSRAVFATDPLSLEPGA
                     ARAAGYADVVAAPTFAIVIQQSCLDALLAEPDTGIDLARVLHGEQRFHYARPVVAGDL
                     LTARLTVTTVRALAGNTMLTAETRIDDERGAEVATATSVLVVQAGDDAGEAAR"
     gene            3820..4242
                     /locus_tag="VFQ96_06980"
     CDS             3820..4242
                     /locus_tag="VFQ96_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU0257565.1"
                     /translation="MTPEFDTLRPGDVVAEREFPLTRESLVRYAGASGDFNPIHYRDD
                     VAARMGLPGVLAQGMLTMGLAVQPVVDWAGDPARIVDYGVRFTRPVVVDPEAGALVAV
                     TATVGALDPESRVARIDLAVTCAGSRVLGKAQAFVRLP"
     gene            4239..5441
                     /locus_tag="VFQ96_06985"
     CDS             4239..5441
                     /locus_tag="VFQ96_06985"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU0257566.1"
                     /translation="MTASRHRRPQPAETGAAEDRGESPVPLSRLTTMGVGGAPRHLIV
                     PSDRQALVRRMRALWERDEPWLLLGGGSNTVAADDGFDGTVVCVATRGIERLPAEGAR
                     PGTVRLRVEAGEVWDDLVVRAVSEGWAGIEALSGIPGRVGAAPVQNIGAYGQELAAVL
                     RSIDFLDRETGETRRIEAEELGFGYRTSAIKRGLAGVVVAIELELDATDALSTPVAYE
                     QLAAALAVPLGTRVPLAAFRHAVLGLRAAKGMLLDPSDPDTRSAGSFFTNPIVDENAA
                     RRLLPVDAPRWPLSLDSPDVVVPLGAPEAAGPVTQPRPAPAFDGAVAVKLSAAWLIER
                     SGIRRGFRLPGSRAAISGKHTLAITNTGGASATEVMQLAAFVRTRVQAEFGITLHPEP
                     VLVGLAFD"
     gene            complement(5546..>6187)
                     /locus_tag="VFQ96_06990"
     CDS             complement(5546..>6187)
                     /locus_tag="VFQ96_06990"
                     /inference="COORDINATES: protein motif:HMM:NF017831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF929 family protein"
                     /protein_id="HEU0257567.1"
                     /translation="ASVLDTVGAKGVAQPPSSVKAPALTANGKPEVLYVGAEYCPFCA
                     AERWPMTVALSRFGTFQGLGLTSSESNDVYPNTATLSFHGATYTSKYVSFVGREIEDR
                     AGKPLDKLTAAEKTVFSTYDSAPYTQSAGAIPFVDVGGKYLIHGASYDPQVLHGKTHA
                     QIAEALHDPTSAIGKNVDAVANVITAAICQTTGGQPAKVCTSAGVASAAATLK"
BASE COUNT          830 a         2194 c         2275 g          888 t
ORIGIN      
        1 tcgctttgcg ttgcgtcgtc gtggtatctg gtggcgcccg tccgggccac gtcgtcgtcg
       61 gcccggccct gctcggccga cgagacgcgg gagcgcccgc cgtgcggcag cgacgcatac
      121 cgggcgcggc tggaggcggg cacccagagg cagccgagga gcgcagccgg gaacgcgaac
      181 gcgatgagcg gcgccagagt cgcggcgccc ccgaacggca gggcggaggg cagcgaccag
      241 acgacggtga ggatcagccc caagacgaac aggatgcccg cgaagacgct gatggcctcg
      301 acggcggacc gcgtcttcgt gcggccgatc gcccacaccg tcacgccgcc cgccgtgagg
      361 agcacgccga ccgcgagggc gatcatccat tccgtgaccg ccagcggcgc gaacgggccg
      421 atcaactgct cgtaggagag ctcgatcgac gagacgatga ccaccaggat gccgcacgag
      481 acggcgagtc cgagaatcac gcgtacgacg gcgaaccagg cggcgccggc gtctgctacc
      541 gccggcagcc gccggccgag cgccggcgcc gcggccgcgg cgccgaaggc cacccagaac
      601 atgagggaga acgtcggtgc cggcccgtgc agcgtcgcgt cgacgagcac gagctgcggg
      661 ttcagcgaca gcaggatgag caccgcggcg gccaccccga gcccgatgcc gatccgccgc
      721 cacgcgcgat ggccggtgag tgcgccgact gcggccacgg ccagaatccc gctggcggcg
      781 aggcccaaca cgatcgacag gatgaacgcg gccggcccgt tcgcggagaa gatcgagaac
      841 atctccgcga tctgcacgag cgtgaagacg acggcgagcc ccgcgacgac caccgtgccg
      901 cgcagccaga cccgcccggc gcggggtatg ggggggagct cccgctgccg cggctgcacc
      961 gccagaatcg cgcccgccat gccgaaggcc agcgccgggc cgagcgtcag ctgtgtgacg
     1021 agcgccagaa cgaggtagac gatgacgagc accgcgtacg gcaggccgat gacgagccgt
     1081 acccggcgca ccgtgtcggg gctccatgcc gtgccgagtg ccggcacacg gccgaggtgc
     1141 gggatggcga gggcgacgag ggtgaccatc gtcacgagca gcacgtcgat gtgtgcggcg
     1201 gcgatgcccg tcgcgacctg cccgtgcggg gcggaggcga cccacggcat gaagagcgag
     1261 gcgacaagga gaaaggccgt gaccgcgtct cgcgcatagt cgcgtgccgc cgcggccgcc
     1321 ggatgcgtgg ccgcgtgtgt ctcgggtcgg ctgcccgccg tggcgcgtgc cgccgtggtg
     1381 gcctcggcca cgcctgtcgg cgccccgcat cgcgggcaga acctggctcc gtcgggcaat
     1441 tgcgctccac acgccgtgca ttgtgccatc tggccgcctt cacctgtcgt cggggtctga
     1501 ggcgtcgggg cttgccacga ccgggcgggc gcctcgtaca gcagactatt ggatatgtcc
     1561 gtgcacacgg tggtggagtc tgcgctgggt gggatcaccc tggtcggcgg cgacgagggt
     1621 ctggaagggc tctatatggc ggagcatcgg cacccccgcc gcgacggatt cgggcaggcc
     1681 gccaccgggc gtgaacggca cgcgctcttc gacgccgcca cggcccagct gggcgagtac
     1741 ttcgccggcg aacgcacccg cttcgacctg gtgctggcgc cccacggcac gcccttccag
     1801 ctgaccgtct ggaatcttct gcgccgcatc gagtacggcc agacctggtc gtatcggcgg
     1861 ctcgccgagg agtacggcaa cccgaaggcc gtgcgtgcgg tcgccgcggc caacgcccgc
     1921 aacccgctca gcatcgtcgt gccgtgccat cgcgtgatcg gcagtgacgg aagcctcgtc
     1981 ggctacggcg gtggcctgtg gcgcaaggag ttcctgctgg agttggaagg cggccgcggc
     2041 accggccagg gcacgctgtt ttgacgggtc gcgcgctctc tcgcctcgcc gcgaacggcc
     2101 cgttcgcggc gcacgccgcg ctcgcggcga tggccgtgca tgccgtgccc ggcctggaac
     2161 gggtcgatgt cggcgccggc ctcgtcgagc ggatgctgtg cttctcgcac ggcccgggcc
     2221 tcgtgcgcat gcaggtctct gactccggcg tcaccgtgga ggcccccgcg gcggtgctcc
     2281 ccgaggccgc cgcccgggtg aggcgatact tcgacctcga gaccgacctc gggcaggtgc
     2341 gggccgtgct cgcccgagat ccgcttctcg ccccgctcgt ggcgcaccgg ccggggctgc
     2401 gcgtcttggg cagcttcgac ccgttcgaat ccgccgcctg catcgtgctc ggccagcagg
     2461 tctccctcgc cgccgcacgc acgctgcagt cccggctcgt ggcggccctc ggcacgcctg
     2521 gctgcgacgg gctgtcgctc tttcccggcc cgcgccgcat cgcggcctgc cccgaacccg
     2581 agctgcgggc ggcgatcggc ctcacccggt cacgggcgcg cacgttgcac ggccttgccg
     2641 aggcgtgtgc cgcgggcctg cgtcttgacg ccccgctgag cgaggtgcgt gccgcccttc
     2701 tgaacctgtg gggcgtcggg ccgtggacgg tcgactttct ctcgctgcgt gcgctcggcg
     2761 accgcgatgc ctgccccgcc ggcgacctgg ttctgcgccg tgcgctcggc atgacgacgt
     2821 cgcgtgatgt tcttgcccgg gcggccggct ggtcgccggt gcgggcctac gcggtgtttc
     2881 atctgtggac cgagatcggc tacgaggccg gcgacacgcc gggccgctga gactcccggc
     2941 cggcggcgga gtgtcggccg cccccggtat cgtgccgcac gtggcgggtc gccggcgaaa
     3001 atggcgaaaa ccactacatg tagtggaatg acaacggcgt gattcccgtt atatagtggt
     3061 gacagcgcga gggtggcacc gcgaggtggg gccgtggcgc gggcgatcat ggcggcggat
     3121 ggcgtggcca ggcgacacag actgggagga tcacatggag tatcaggatg acgtcgtcga
     3181 cggctactcg gttcccgtcg acccaatgga ctttctccag tgcgacagct gccagtgagg
     3241 cgctgacgca ccttggcatc cggctgcaga aggccggttc tcccgaagcc ccgaccacga
     3301 ggtcggggct tcggcgtctg cggggtgcgc cccgcatgtt tcgtacactg gacgggtgag
     3361 cgtgaaccct gagctggccg ggcgggttct gccgccccgg ggcccgtacc tcgtcggccg
     3421 ggagaaggtg cgcgagttct cgcgggcggt cttcgccacc gacccgttga gcctcgagcc
     3481 gggtgcggcc cgcgcggccg gctacgccga tgtcgtcgcc gcgcccacct tcgcgatcgt
     3541 catccagcag tcgtgcctcg acgcgctcct cgccgaaccc gacaccggca tcgacctcgc
     3601 ccgcgtcttg cacggcgagc aacgcttcca ctacgctcgc ccggtcgttg ctggcgacct
     3661 tctgacggcg cggctgaccg tgacgacggt gcgggcactg gccggcaaca cgatgctgac
     3721 cgccgagacc cgcatcgacg acgagcgcgg agcagaggtc gcgaccgcca cctcggtgct
     3781 cgtggtacag gccggcgacg acgcggggga ggcggcgcgg tgacgccgga gttcgacacc
     3841 ttgaggccgg gcgacgtggt ggcggagcgc gagtttcccc tcacccggga gtcgctggtg
     3901 cgctacgcgg gtgcctccgg agacttcaac cccatccatt accgcgacga cgtggcggcg
     3961 cggatgggcc tgcccggcgt tctcgcccag ggcatgctca ccatggggct cgcggtgcag
     4021 cccgtcgtcg actgggccgg cgacccggcc cgcatcgtcg actacggcgt gcgcttcacg
     4081 cgccccgtcg tcgtcgaccc ggaggccggt gccctcgtcg cggtgacggc gacggtcggg
     4141 gccctcgacc ccgagagccg cgtcgcgcgc atcgaccttg ccgtcacctg cgccggctcg
     4201 cgcgtcttgg gcaaggcgca ggccttcgtg cgtctgccat gaccgcctca cggcaccgcc
     4261 ggccgcagcc ggccgagacg ggcgcggccg aagaccgcgg cgagtcgccc gtacctcttt
     4321 ctcggctgac gacgatgggg gtcggcgggg cgccgcgcca cctcatcgtg ccctccgacc
     4381 ggcaagcgct ggtgaggcgc atgcgggcgc tgtgggagcg cgacgagccc tggctgctcc
     4441 tcggcggcgg atcgaatacc gtcgcggcgg acgacggctt cgacgggacg gtggtgtgcg
     4501 tcgcgacgcg gggaatcgag cgcctccccg ctgagggggc ccggcccggc acggttcgcc
     4561 tgcgggtcga ggccggcgag gtgtgggacg acctggtggt gcgtgccgtc agcgagggat
     4621 gggcgggcat cgaggcgctg tcgggcatcc ccggccgggt gggcgccgcg ccggtgcaga
     4681 acatcggcgc ctacggccaa gagcttgccg cggtgctgcg cagcatcgat tttctcgacc
     4741 gggagaccgg cgagacccgg cgtatcgagg ccgaggagct cggcttcggc taccgcacct
     4801 cggcgatcaa acggggcctc gcgggcgtcg tcgtcgcgat cgagctggag ctcgacgcga
     4861 ccgacgccct cagcaccccg gtcgcctacg aacaactggc cgccgcgctc gcggtgccgc
     4921 tcggcacgcg cgtgccgctt gccgcgttcc gccacgccgt gctcgggctg cgtgcggcga
     4981 aggggatgct gctcgacccc tccgaccccg acacgcgaag tgccgggtcg tttttcacca
     5041 atccgatcgt cgacgagaac gcggcgcggc ggctgttgcc ggtcgacgcg ccccgctggc
     5101 cgctctcgct cgattcaccc gacgtcgtcg tgccgctcgg ggcccccgaa gcggccggcc
     5161 cggtgacgca gcctcgcccg gcgcccgcgt tcgacggggc cgtggcggtc aagctcagcg
     5221 cggcctggct gatcgagcgg tcgggcatcc gccgcggatt ccggctgccc ggttcccgcg
     5281 ccgcgatctc cggcaaacac acgctcgcca tcacgaacac gggcggcgcg agcgcgaccg
     5341 aggtgatgca actcgccgcg ttcgtgcgca cccgggtgca ggcggagttc ggcatcacgt
     5401 tgcaccccga gcccgtgctc gtcggcctcg ccttcgacta ggcgggcgca cgccggcctg
     5461 cggcgcccgg acggccacga tccttgctcc ttatggggcg cctgcaggcg gtagccgggc
     5521 gtcggggccg cgacttcgcc gggcgctatt tgagcgttgc cgcggccgag gcgacccccg
     5581 cgctcgtgca gaccttcgcc ggctgcccgc ccgtcgtctg gcagatcgcg gccgtgatga
     5641 cgttcgccac cgcgtcgacg ttcttgccta tcgcggaggt cgggtcgtgc agagcctcgg
     5701 cgatctgtgc gtgcgtcttg ccgtgcagca cttgtggatc gtaagaggcg ccgtggatca
     5761 gatacttgcc gcccacgtcg acgaacggga tcgcgccggc cgactgcgtg tagggggccg
     5821 agtcgtaggt ggagaacacc gtcttctcgg cggcggtcag cttgtcgagc ggcttgccgg
     5881 cgcggtcttc gatctcccgg cccacgaacg agacgtactt gctcgtgtag gtggcaccgt
     5941 ggaagctcag cgtggcggtg ttggggtaga cgtcgttcga ctccgacgag gtcaggccga
     6001 gaccctgaaa ggtgccgaac cgagagagcg ccacggtcat cggccaacgc tctgcggcac
     6061 agaacgggca gtactcggcg ccgacgtaga gaacctcggg cttgccgttc gccgtcaggg
     6121 ccggcgcctt gaccgagctg ggaggctgcg cgacgccctt cgccccgacc gtgtcgagca
     6181 ccgaggc
//
LOCUS       DASTNL010000218         7456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1088089, whole genome shotgun sequence.
ACCESSION   DASTNL010000218 DASTNL010000000
VERSION     DASTNL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088089"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2707)
                     /locus_tag="VFQ96_06995"
     CDS             complement(<1..2707)
                     /locus_tag="VFQ96_06995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0257568.1"
                     /translation="MPTTVTAVVVYPGGGDLERTLAALAGQTRRPDRVVVVKTSSRLP
                     QPPERLAEGVNAVVTAHENATFGAAVAAGLAERADLPAEDEFYWLLDHDVFPDGRALA
                     ALLAAMEASPSVGVAGPKLVDADDPERLRGLGETLTSLGASVTLMTGGPTGLDGVGVG
                     SDYAAGELDQGQHDHISDVLGVSARGMLVRAGLWRRVDGFDVALEAADDGLDLCVRAR
                     LCRYRVVVVPEARVAVAAAGRKRAWHRSRQRHRRYRRRRRAQLHRRLVYAPALALPLH
                     WLSLPPLGLVRILLWLVCKRPGLVFPEIAATLRVFFSWRAVGRARRTLRRERDVGFRV
                     IAPLRLHLGDARRMRALARAAGRRRDAADDEEALFTEAGFWAIIAAALAGVILALPIL
                     GRASLAGGGILPLGSFHALWSAAAHGLRPYDFVLAVLGTPVFWQPSLAFAALWVAALP
                     LAALGAWFAATTLTRRAGLRAAIALAWAFAPPLLAALYDGRSGALVFHLLLPWIVVAA
                     AHIPRSWSAVGTAALLVAAALAAAPSAWPLFAAAWLVGLAVAGRSLGRALALPLPALC
                     LFAPAAWRAWPNLPALFADPGVPVVGSPGAPWQVLLGLPPAAAARLALLVHDVTPAAW
                     GQLSLPMIVAALLFAVPALIALAATFLSHAGRAGLWLGLALAGMVVALVVGHVPSGFV
                     GEQPVTIAPGPALSVAWLGILAAGVTGLDVLAAWAAARRVRRHPTTRRRDASVGIGVL
                     TWAALCSVALASLPVAIALTAGHGQVAAATRSALPAVVTAQASTNPQVGTLRLTAQPG
                     GGLGATVARGAGQSLAGLAQAGADPGLARLVGDLATRGHAGTSAGLKGRGIGFVLVMA
                     PARAPGAGVTAAARATRSRVLGALDADATLQRVQAAGGERLYSVAG"
     gene            complement(2786..3118)
                     /locus_tag="VFQ96_07000"
     CDS             complement(2786..3118)
                     /locus_tag="VFQ96_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU0257569.1"
                     /translation="MTTDLQPHVPDDWFVDPLHTGFPSRPGTRFPVEAGETEDPLAWQ
                     ADALCAQTDPEIFFPEKGGSTREAKKICEQCPVRQQCLQYALQNDERFGIWGGLSERE
                     RRRLRRAG"
     gene            complement(3225..4190)
                     /gene="galE"
                     /locus_tag="VFQ96_07005"
     CDS             complement(3225..4190)
                     /gene="galE"
                     /locus_tag="VFQ96_07005"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU0257570.1"
                     /translation="MTWLVTGGAGYIGAHVVRAFLAEGIDTIVLDDLSSGHDGFVPDG
                     VRLVRASTLDRDALDRLFAKAHIDGVVHVAGFKYAGVSVQRPLHTYRQNVTGTAVLLE
                     AMRDAGVGRVVFSSSAAVYGTPDVDIVTETTAKRPQSPYGESKLVGEWLLHDAGVAHG
                     LAHTSLRYFNVVGSGSPELRDTSPHNLFPLVFDALAAGRVPQINGDDYPTPDGTCVRD
                     YVHVADLARSHVAAARRLQEGLPVEEAYNLGSGDGVSVRQIMTAVAQVTGIAFEPSLA
                     PRRPGDPARIVASGELAARDLDWRMRHSLTQMVDSAWQARLAADQ"
     gene            complement(4310..5668)
                     /gene="manA"
                     /locus_tag="VFQ96_07010"
     CDS             complement(4310..5668)
                     /gene="manA"
                     /locus_tag="VFQ96_07010"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU0257571.1"
                     /translation="MLVPIANTPRPYAWGSHTAIAELFGRPPSGAPEAEVWFGAHPGS
                     PSRIVDTTLSGGAASLDAWIRRDPAAALGPAWRPGSEPALEAPRLPFLLKVLAAERPL
                     SLQVHPSAAQARAGHARDDEAGLAPDDPARDYRDPYPKPEMIVAVSRFDALCGFRPLD
                     ACRRLVRQLSEVGGLGADRRAALAALAARLAPPAVDVAPGGQATPVGRALAGAVEWIL
                     AGSAGPALVEAVVAQARRIADGVDADRDAELQAAARLTLDLAEYHPGDPGIVLSLLLN
                     RVCLQPGEALFLPAGRIHAYLHGLGIELMVSSDNVLRGGLTHKHVNVPELVRVLDATP
                     RPARPFTAAATGPGVWEYRPDATFRLARLRAGAGVSPAFPLPGAPAVLLCLSGEVVAF
                     GTAGGGAPGAGEGRAWPLTPGQGLYVTPDETVLRLAGTGEAVLATTALPTGGDPLDAA
                     PQ"
     gene            5683..7173
                     /locus_tag="VFQ96_07015"
     CDS             5683..7173
                     /locus_tag="VFQ96_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0257572.1"
                     /translation="MSHPSWTTDAVTVSTNSGNTLYAMIAQPSPPGHRSVLTGQAGPA
                     GSFTRLVATLALFTGLAGGFWRDFIGWWGWGALAAAIVVTSIVLLTRRRRVSLPPVWL
                     GLFLVLIIASVGWSDYRAVTVLADIAQLSTTAVGAFLALALSWAELVRALANALRWIV
                     GLSLLFELVVSLLVRHQILPFWTNYGPGPHPEAFYWSRNVLLHGGRIQGIVGNSNILG
                     MCALLAVIVFGAQLADGAVRRMRGVSWLVAAALCVVLTRSSTVVAAAFVVLLAFVFAV
                     WCRHRPEEHRRRVYVTAAASAVAAILAVLALRGPLLALLGKTDTLTGRTVIWHSVAQL
                     ARQRPAFGWGWTSYWAPWVKPFKGLAVQDGVEYLQAHDAWLDVWMQVGIVGLVIFAAL
                     CATTLWRLWFLAVDSPKVPAAHGDTARRALALVPFLIFVAYLAQSLAESRLLIEGGWM
                     LLVAFSLKSAQGQIDPGPGADAPGDDARRLARPHGHESGEALQGRR"
     gene            7170..>7456
                     /locus_tag="VFQ96_07020"
     CDS             7170..>7456
                     /locus_tag="VFQ96_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257573.1"
                     /translation="MTAEAFYRRAGVASRFLAAPRFTAALSLGIVAVAFGTYLIRSVI
                     GWPGLIGAVASLVLLASASLLAQLRFIEWTGLLPVSVLCFIVWCGASVFWSA"
BASE COUNT         1037 a         2669 c         2792 g          958 t
ORIGIN      
        1 cgcccgccac gctgtacagg cgttcgccgc cggccgcctg cacccgctgc agggtggcgt
       61 cggcgtcgag cgcgccaagc acacgggatc tcgtcgccct ggccgccgcc gtgacgcccg
      121 ccccgggtgc ccgggcgggc gccatgacga gcacgaagcc gatgcccctg cccttcagcc
      181 cggcgctcgt gccggcgtgg ccgcgggtgg cgaggtcgcc gacgaggcgc gccaggcccg
      241 ggtcggcccc ggcctgcgcg agccctgcga ggctttgccc ggcgccgcgc gctaccgtcg
      301 cgcccaggcc gccgccgggc tgggccgtga ggcgtagcgt gccgacctgc gggttggtcg
      361 acgcctgcgc cgtcacgacg gcgggcaggg cggatcgggt ggctgccgcg acctgaccgt
      421 ggcccgcagt gagggcgatc gcgacgggca gcgaggcgag ggccaccgag cagagcgccg
      481 cccacgtgag aacgccgatg ccgacggacg cgtcacggcg ccgcgtcgtg ggatgccgcc
      541 gcacccgccg ggccgcggcc caggccgcca gcacgtcgag gccggtcacg ccggccgcga
      601 gaatcccgag ccacgccacg ctgagtgcgg ggcccggcgc gatcgtcacc ggctgttcgc
      661 cgacgaagcc agaaggcacg tggccgacga cgagcgccac gaccatgccc gccagagcga
      721 ggccgagcca gaggccggcc cgaccggcgt gagaaagaaa ggtggcggcg agggcgatga
      781 gcgcgggcac ggcgaagagg agggccgcga cgatcatcgg cagggaaagc tgcccccacg
      841 cggcgggcgt cacgtcgtgg accagtaagg ccagccgggc ggcggcggcc ggcggcagcc
      901 ccaggagcac ctgccacggg gcgcccggcg agcccaccac cggcaccccc gggtcggcga
      961 agagcgccgg caggttcggc caggcacgcc aggccgcggg agcgaaaagg cagagcgccg
     1021 gcagcggcag ggcgagcgcg cggccgaggc tgcggcccgc caccgcgagg ccgacgagcc
     1081 aggccgcggc gaagagcggc caggcggagg gcgccgcggc cagcgccgcc gccacgagaa
     1141 gggcggccgt gccgaccgcc gaccaggaac gggggatgtg cgctgcggcg acgacgatcc
     1201 acggcagcag caggtggaac accagcgcgc cgcttcgccc gtcgtagagc gccgcgagaa
     1261 ggggcggggc gaacgcccac gcgagcgcga tcgccgcccg caggccggcg cggcgggtga
     1321 gcgtggtcgc ggcgaaccag gcgcccagag ccgccagcgg caacgccgcc acccagagtg
     1381 ccgcgaaggc gagggagggc tgccagaaca ccggcgtgcc aagcacggcg agcacgaaat
     1441 cgtacggccg aaggccgtgg gcggcggcgg accacagggc gtggaacgag ccgagcggca
     1501 gaattccacc gccggcgagg ctcgcccggc cgagaatcgg cagagcgagg atgacgccgg
     1561 ccagcgcagc cgcgatgatc gcccagaacc cggcctcggt gaagagcgcc tcctcgtcgt
     1621 ccgccgcgtc tcgccgccgc cccgcggcgc gcgcgagcgc ccgcatacgc ctggcgtcgc
     1681 cgaggtgcag gcgcaggggc gcgatgaccc tgaacccgac gtcgcgctcc cgtcggaggg
     1741 tgcggcgcgc ccgccccacg gcacgccaag agaagaaaac gcgcagcgtg gcggcgatct
     1801 cgggaaagac gagaccgggg cgcttgcaca cgagccagag gaggatgcgc acgaggccca
     1861 gaggcggcag gctcagccaa tgcaacggca aggcgagggc gggagcatag acgagacggc
     1921 ggtgtagttg agcccgacgc cgccggcgat agcggcggtg acgttgccgg ctgcggtgcc
     1981 aggcacgttt gcgaccggcc gcggctaccg cgacccgagc ctccggcacg acgacgacgc
     2041 gataccggca cagccgcgcc cgcacgcaca ggtcgagtcc gtcgtcggcc gcctccaacg
     2101 cgacgtcgaa cccgtcgacg cgccgccaca ggccggcgcg cacgagcatg ccgcgcgccg
     2161 agacgccgag cacgtcgctg atgtggtcgt gctggccttg gtctagttcc cccgccgcat
     2221 agtcgctgcc gacgcccacc ccgtcgaggc cggtcggccc gccggtcatg agggtgacgg
     2281 aggcaccgag actcgtcagc gtctcgccga ggccgcggag ccgttcgggg tcgtcggcgt
     2341 cgacgagctt cggcccggcg acgccgaccg aaggcgatgc ctccatcgcg gccaagagcg
     2401 ccgccagcgc ccgcccgtcg gggaacacgt cgtgatcgag aagccagtag aactcgtcct
     2461 cggcgggcag gtcggcccgc tcggccaggc ctgccgcgac ggcggcgccg aacgtggcgt
     2521 tctcgtgggc cgtgacgacc gcgttcaccc cttcggcgag ccgctcgggc ggctgcggca
     2581 ggcgcgacga cgttttgacc accacgacac ggtcggggcg gcgggtctgg ccggccaacg
     2641 cggcgagggt acgttcgagg tcgccgccgc ccgggtatac gacgacggcc gtcacggtgg
     2701 tgggcatcgc cgaaagccta tgcgtcaacg gccccttggc ctgtgaggcg ccgggcatgt
     2761 cggccggcgg cgggcccggc agcgttcagc cggcgcgacg cagccggcga cgttctcgct
     2821 cggagagccc gccccagatg ccgaagcgct cgtcgttctg cagcgcatac tgcaggcatt
     2881 gctggcgcac cgggcactgc tcgcagatct tcttggcctc ccgggtggag ccgcccttct
     2941 cggggaagaa gatctccggg tcggtctgcg cgcagagcgc gtccgcctgc cacgcgagcg
     3001 ggtcttccgt ctcgccggcc tcgaccggga aacgcgtgcc ggggcgggag gggaaccccg
     3061 tgtggagggg atcgacgaac cagtcgtcgg ggacgtgcgg ctgaagatcc gtcgtcatag
     3121 caccctccca ctcgcgtccc gggatatcgg gcgggacacg ccgtacataa ttacagtcgt
     3181 gtaattcatc ggcgtcaagt cccgacgggt tattgggccg gcgttcactg atcggcggcc
     3241 aggcgcgcct gccaggcgct gtcgaccatc tgggtgaggc tgtgccgcat gcgccagtcg
     3301 aggtcgcggg cagcgagctc ccccgaagcg acgatccgcg ccgggtcacc gggccgacgc
     3361 ggcgccaagg aaggctcgaa cgcgataccc gtcacctggg cgacggcggt catgatctgc
     3421 cgcaccgaca cgccgtcgcc gctgcccagg ttgtaggcct cttccaccgg cagaccctct
     3481 tgcaggcggc gggcggccgc cacatgggaa cgggccaggt cggcgacgtg cacatagtcg
     3541 cgcacgcagg tgccgtcggg ggtggggtag tcgtcgccgt tgatctgcgg cacacggccg
     3601 gccgcgaggg cgtcgaagac gagcgggaag aggttgtgcg ggctcgtgtc gcgcagctcg
     3661 gggctgccgg agcccaccac attgaagtag cgcaggctgg tatgggcgag gccgtgcgcg
     3721 acgccggcgt cgtgcagcaa ccactcgccg acgagtttcg actcgccgta cggcgactgc
     3781 ggcctcttcg ccgtcgtctc ggtgacgatg tcgacgtcgg gggtgccgta gacggcggcg
     3841 gaggaagaga agacgacccg gccgacgccg gcgtcgcgca tcgcctccag cagcacggcc
     3901 gtgccggtga cgttctgccg gtaggtgtgc aacgggcgtt gcaccgacac gccggcgtac
     3961 ttgaagccgg cgacgtggac gacgccgtcg atgtgagcct tcgcgaagag gcggtcgagc
     4021 gcatcgcggt cgagggtcga ggcccgcacg agccgcacgc cgtccggcac gaagccgtca
     4081 tgcccgctgg agaggtcgtc gaggacgatg gtgtcgatcc cctcggcaag gaaggcccgc
     4141 acgacgtgcg ccccgatgta cccggccccg cccgtcacaa gccacgtcat gcgctcttcc
     4201 cctccggccg actccccgac ccgctctgcc gtctctgccc gctgccgcat cgacaccgta
     4261 tcaaccgcgg agcagcgatc ctgtcacggc acgaccgcca cccaagccgt cactgcggtg
     4321 ccgcatcgag tggatcacct ccggttggta gggcggtcgt ggcgagcacc gcctcgcccg
     4381 tgccggcgag gcgcaagacg gtctcgtcgg gggtgacgta gaggccctgg cccggggtga
     4441 gcggccaggc ccgcccttcg ccggcgccgg gggcgccgcc tccggccgtg ccgaaagcga
     4501 cgacctcccc actcaggcag agcagcacgg cgggcgcgcc gggcagcggg aaggccggcg
     4561 agacacccgc ccccgcccgc agccgcgcga gccggaacgt ggcgtcgggc cgatactccc
     4621 agacgccggg cccggtggcg gccgccgtga agggacgggc cggcctcggc gtcgcatcga
     4681 gcacccgcac gagctccggt acgttcacgt gcttgtgggt gaggccgccg cgcagcacgt
     4741 tgtcggaaga gaccatgagc tcgatcccga ggccgtgcag gtaggcgtgg atccgcccgg
     4801 ctggcaggaa gagggcctcg ccaggctgca ggcagacacg gttcaagagc aggctcaaga
     4861 cgataccggg gtcgcccggg tggtactccg cgaggtcgag cgtcaaccgt gccgccgcct
     4921 gcaactccgc atcccggtcg gcgtcgacgc cgtcggcgat acgccgcgcc tgggccacga
     4981 ccgcctcgac caaggcgggg ccggccgagc cggcgagaat ccattcgacg gcgccggcaa
     5041 gcgcccggcc gacgggagtg gcctggccgc cgggtgccac atcgacagcc ggcggcgcca
     5101 gccgcgcggc gagggcggcg agcgcggcgc gccggtcggc gccgagcccg cccacctcgc
     5161 tcaactgtcg aacgagcctg cggcacgcat cgagcgggcg aaagccgcac agcgcgtcga
     5221 agcggctgac ggcgacgatc atctcgggct tcgggtatgg atcgcggtag tccctcgccg
     5281 ggtcgtcggg agcgaggccg gcctcgtcgt ctcgcgcatg accggcgcgg gcctgggccg
     5341 cgctcggatg cacctgcagg gagagcgggc gctcggcagc gagcactttg agaaggaacg
     5401 gcagccgcgg cgcctcgaga gcaggttcgg agccgggccg ccacgccggg ccgagtgccg
     5461 cggccgggtc ccgacggatc cacgcgtcga ggctcgccgc cccgccgctg agagtcgtgt
     5521 cgacgatgcg gctcggggag cccgggtggg cgccgaacca gacctccgcc tccggagcgc
     5581 cgctgggcgg cctgccgaac agctccgcga tcgccgtgtg agaaccccac gcgtagggcc
     5641 gcggcgtgtt ggcgatgggc acgagcatgc gcacccctcc ccttgtcgca tccttcgtgg
     5701 acgaccgacg cggtcaccgt atcaacaaac tcgggcaata cgctgtatgc catgatcgcg
     5761 cagccgagcc cgcccggcca tcgcagcgtg cttaccggcc aggcgggccc ggcagggtct
     5821 ttcacgcgcc ttgttgcgac gctggccttg ttcaccgggc tcgccggggg cttctggcgt
     5881 gacttcatcg gctggtgggg ctggggcgca ctcgccgcgg ccatcgtcgt cacctcgatc
     5941 gtgcttttga cacgcaggcg ccgtgtttcg ctgccgcccg tgtggctggg gctcttcctc
     6001 gtgctcatca tcgcgtcggt cggctggtcc gactaccgtg ccgtgacggt gctggccgac
     6061 atcgcgcagc tctcgacgac ggcggtcggg gcgttcctgg ccctggccct gtcgtgggcg
     6121 gagctggtgc gggccctcgc gaacgccctg cggtggatcg tggggctctc gctcctcttc
     6181 gagctggtcg tgtctctcct cgtgcggcac cagattttgc cgttctggac gaactacggg
     6241 ccgggtccac acccggaggc cttctactgg agccggaacg tgcttctcca cggcggccgc
     6301 attcagggga tcgtcggcaa cagcaacatc ctcggcatgt gcgcgcttct ggcggtcatc
     6361 gtgttcggcg cccagttggc cgacggcgcg gtgcgccgga tgcggggcgt ctcctggctc
     6421 gtcgcggccg cgctctgcgt cgttctcacc cgttcgtcca cggtcgtggc ggcggccttc
     6481 gtcgtgttgc tggcattcgt cttcgccgtg tggtgccggc atcggccaga ggagcaccgg
     6541 cgcagggtgt acgtgaccgc ggcggcctcg gcggtggcgg ccatcctcgc ggtgttggcg
     6601 ctgcgcggcc cgcttctggc cctgctcggc aagaccgaca ccttgacggg ccgcaccgtg
     6661 atctggcatt cggtcgccca gctcgcccgg cagcggccgg ccttcggctg gggctggacg
     6721 agctactggg cgccgtgggt gaagccgttc aaagggctcg ccgtgcaaga cggggtggag
     6781 tacctgcagg cgcacgacgc atggctcgac gtctggatgc aagtgggcat cgtcggcctc
     6841 gtgatcttcg ccgcgctgtg cgccacgacc ctgtggcgac tctggttctt ggcggtcgac
     6901 agccccaagg tgccggccgc ccacggtgac acggcgcggc gtgcgctggc cctcgtgccg
     6961 ttcctcatct tcgtcgccta cctcgcgcag tccctggccg agagcaggct gctcatcgag
     7021 ggcggctgga tgctgctcgt cgccttcagc ctgaagtcgg cccaggggca gatcgacccg
     7081 ggccccggcg cagacgcgcc gggcgacgac gcacgacgcc tcgcccggcc gcacggccac
     7141 gagtccggcg aggcgctcca gggccgccga tgacggcgga ggcgttctac cgccgcgccg
     7201 gcgtggcctc gcgcttcctc gccgcgccgc gattcacggc ggcgttgagc ctcgggatcg
     7261 tggcggtcgc gttcggcacc tacctgatcc gcagcgtgat cggctggccg gggctcatcg
     7321 gcgccgtggc gagtctcgtg ctgctcgcct ccgcctcgct cctcgcccag ctgcgcttca
     7381 tcgagtggac cggcctcctg ccggtctcgg tgctgtgctt catcgtgtgg tgcggggcga
     7441 gcgtcttctg gagcgc
//
LOCUS       DASTNL010000219         4281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1088197, whole genome shotgun sequence.
ACCESSION   DASTNL010000219 DASTNL010000000
VERSION     DASTNL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088197"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="pgk"
                     /locus_tag="VFQ96_07025"
     CDS             complement(<1..545)
                     /gene="pgk"
                     /locus_tag="VFQ96_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU0257574.1"
                     /translation="MTLRTLAALRPLAGKRVVVRCDLNVPIAEGKIGDDGRVRASLPT
                     LNALRRAGARVVVIAHLGRPKGPDEALSLAPVARRLEELLGAPVRFATDTVGPEAART
                     VEALQDGDVAVLENLRFSPDETSKDEAVRTRFARRLAAFGDAFVSDGFGVVHRAQASV
                     VDLARLLPSAAGTLVERETGVL"
     gene            complement(562..1566)
                     /gene="gap"
                     /locus_tag="VFQ96_07030"
     CDS             complement(562..1566)
                     /gene="gap"
                     /locus_tag="VFQ96_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU0257575.1"
                     /translation="MSVKIGINGFGRIGRNYFRAALAKGSDLDIVAVNDLTDSKTLAH
                     LLKYDSVGGRLGAEVTTDGDDIVVNGKAIKVLAERDPANLPWKELGVDIVIESTGFFR
                     KAADARKHIEAGAKKVIISAPASGEDITLVLGVNEDKYDPANHDIISNASCTTNCLAP
                     LAKVLLDNFGIERGFMTTVHAYTADQKLQDGPHNDLRRARAAAINTVPTSTGAAKAIG
                     LVLPELVGKLDGFALRVPVPTGSITDLTVEVSRPATVDQINAAYRAAADGPLKGILGY
                     TSDPIVSGDIVADPRSSIFDSGLTKVIGNQVKVVAWYDNEWGYSNRLVDVTEYIAKRL
                     "
     gene            complement(1665..2291)
                     /locus_tag="VFQ96_07035"
     CDS             complement(1665..2291)
                     /locus_tag="VFQ96_07035"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU0257576.1"
                     /translation="MAEYTLADLPYDYSALEPHISGTIMELHHDKHHAGYVKGANTAI
                     EKLAAARDANDFTNITKLQKDLAFNLAGHVNHTVFWKNLSPDGGDKPQGELAAAIDEF
                     FGSFDKFRAHFTASALGIQGSGWSILAWDALGGKLIIEQLYDHQGNLATSTVPLLMID
                     MWEHAFYLDYKNVKGDYVKAYWNIVNWADVARRFEAARQKTSGLLLLS"
     gene            complement(2411..3397)
                     /gene="whiA"
                     /locus_tag="VFQ96_07040"
     CDS             complement(2411..3397)
                     /gene="whiA"
                     /locus_tag="VFQ96_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU0257577.1"
                     /translation="MALTADVKAELVVFPITKTRVRAAELTATLRFSGGLHRISGRIA
                     VESELDSPELAVRVRKTLAEVYAVRSDVAVIAGGDGKHSRHFIVRVVAGGETLARQTG
                     LLDLRHHPVRGLPNKLTTGNPTELAAVWRGAFLAAGTLSDPGRSAALEVASPGNEAAM
                     ALAGAAIRLGVSAKSREVRGTHRVVVRDGEAIARLLTLMGAHHTVARWQEMRKRREVR
                     ASANRLVNFDDANLRRSAQAAVTACARVERAFEILGDDVPEHLRYAGSLRLTHREASL
                     DELGRRAEPPLTKDAVAGRIRRLLAMADKRAQQQGVPGTEAALPMPAAADEV"
     gene            complement(3465..>4281)
                     /gene="rapZ"
                     /locus_tag="VFQ96_07045"
     CDS             complement(3465..>4281)
                     /gene="rapZ"
                     /locus_tag="VFQ96_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU0257578.1"
                     /translation="RSTVANALEDLGWYVVDNLPPQMLRPLIELASRAGERLPKIAAV
                     VDVRGRDFFADIEDIVHSLRLGTRARILFLEATDEALVRRFESVRRPHPLQGQGTLLD
                     GIAAERTRLTDLRAASDIVIDSSDLNIHQLATSVATLFATGDRDEMRVTVMSFGFKYG
                     VPTDADMVVDTRFLPNPFWVPELRHHTGLDEDVAQYVFASASAGPFLDKYNEVLRLVI
                     DGYRAENKRHLVVAVGCTGGKHRSVAIAEELGRRLTQMPSVTSSVQHRDLGRE"
BASE COUNT          629 a         1407 c         1499 g          746 t
ORIGIN      
        1 agcacgccgg tctcccgttc gacgagcgtt cccgccgcgc tgggcaggag cctcgcgagg
       61 tcgacgacgc tggcctgtgc gcgatgcacc acgccgaacc cgtcggaaac gaaggcgtcg
      121 ccgaacgcgg caagacggcg cgcgaacctc gtgcgaaccg cctcatcttt gctcgtctcg
      181 tcggggctga accgcaggtt ctcgagaacc gcgacgtcgc cgtcctgcag cgcctcgacc
      241 gtgcgggcgg cctccggccc gaccgtgtcc gtggcgaaac gcaccggggc gcccaggagc
      301 tcctccagcc gtcgggcgac tggcgcgagg ctgagggcct cgtccggccc cttcgggcgc
      361 ccgaggtggg cgatgacgac gacccgtgca ccggcacgcc gcaacgcgtt cagggtgggc
      421 agtgacgcgc gcaccctccc gtcgtcgccg atcttcccct cggcgatcgg tacgttcagg
      481 tcgcagcgaa ccacgacgcg tttaccggcc agcgggcgaa gcgctgccaa ggtacgtaac
      541 gtcacgtgcc tgtccccgaa cttaaaggcg cttggcgatg tactcggtga cgtcgacgag
      601 gcggttcgag tagccccact cgttgtcgta ccaggcgacg accttgacct ggttgccgat
      661 gaccttggtc agcccggagt cgaagatcga cgagcgcggg tcggcgacga tgtcacccga
      721 gacgatcggg tccgacgtgt agccgaggat ccccttgagc gggccgtctg cggcggcccg
      781 gtaggccgcg ttgatctggt cgacggtggc cgggcggctc acctcgacgg tcaggtcggt
      841 gatggagccg gtcggcaccg gcacccgcag tgcgaaaccg tcgagcttgc cgacgagctc
      901 cggcaggacg aggccgatcg ccttcgcggc gccagtcgag gtcggcaccg tgttgatcgc
      961 cgcagcgcgt gcgcggcgca ggtcgttgtg cgggccgtct tgcagcttct ggtcggcggt
     1021 gtaggcgtgc acggtcgtca tgaagccgcg ctcgatgccg aagttgtcga gcagcacctt
     1081 ggcgagcggc gcgaggcagt tcgtcgtgca ggaggcgttt gagatgatgt cgtggttcgc
     1141 cgggtcgtac ttgtcctcgt tgacgcccag caccagggtt atgtcttccc cggaggccgg
     1201 cgccgagatg atgaccttct tcgccccggc ctcgatgtgc ttgcgggcat cggcggcctt
     1261 gcggaagaaa ccggtcgact cgataacgat gtcgacgccg agctctttcc acggcaggtt
     1321 cgcgggatcg cgctcggcca gcaccttgat cgccttgccg ttcacgacga tgtcgtctcc
     1381 gtcggtcgtg acctccgcgc ccagccgacc gccgaccgag tcgtacttca gcaggtgggc
     1441 gagcgtcttg ctgtcggtaa ggtcgttgac cgcgacgatg tcgaggtcac tgcccttggc
     1501 cagggcggca cggaaatagt tgcgaccgat gcggccgaaa ccgttgatcc cgatttttac
     1561 ggacacgtag tactcctgat tggtgatgcg ctccaggcgc gatgacatgg acaggccgtg
     1621 acggcggtgt cccgggtggc ccggcaccgg gacaccgcga cccgttacga cagtagcagc
     1681 agccccgacg tcttctgcct ggcggcctcg aaccggcgtg ctacgtcggc ccagttgacg
     1741 atgttccagt aggccttgac gtagtcgccc ttgacgttct tgtagtccag gtagaaagcg
     1801 tgttcccaca tgtcgatcat gaggagtggc acggtcgagg tggcgaggtt gccttggtgg
     1861 tcgtacagct gctcgatgat gagcttgccg cccagcgcgt cccacgcgag gatcgaccat
     1921 ccggagccct ggatgccgag cgccgaggcg gtgaagtgtg cgcggaactt gtcgaacgag
     1981 ccgaagaact cgtcgatcgc tgcggccagc tcgccctgcg gcttgtcgcc gccgtcgggc
     2041 gagaggttct tccagaacac cgtgtggttc acgtgcccgg ccaggttgaa ggcgaggtct
     2101 ttttgaagct tcgtgatgtt ggtgaagtcg ttcgcgtcac gggccgcggc cagcttctcg
     2161 atcgcggtgt tcgcgccttt cacatagccg gcatggtgct tgtcgtggtg aagttccatg
     2221 atggtgccgc tgatatgcgg ctcaagagcg gaatagtcat atggcagatc tgccagtgtg
     2281 tactcggcca ttctcggtga cctcctcgag ggtcgtgtgc gccagaagtc ttcgggcgct
     2341 gtcggaacaa cgtttttgag tctaggccgt atggccgccg ggcacggacg gtgcccgtgt
     2401 gtgcccggcc tcagacctcg tcggccgcgg ccggcatggg cagcgcggcc tcggtgcccg
     2461 gcacgccctg ctgttgggcg cgcttgtcgg ccatcgccag aaggcgccgg atgcggccgg
     2521 cgacggcgtc tttggtgagc gggggctccg cccgacgtcc gagctcgtcg aggctcgcct
     2581 cccggtgcgt cagacgcaaa gatccggcgt agcgcaggtg ctccgggacg tcgtcgccga
     2641 ggatctcgaa ggcgcgctcg acgcgggcgc aggcggtgac cgcggcctgt gccgaacgcc
     2701 gcaagttcgc gtcgtcgaag ttgacgagcc ggttggcgct cgcgcgcacc tcgcgacgct
     2761 tgcgcatctc ctgccaccgg gccaccgtgt gatgcgcgcc catcaaggtc agaagacgtg
     2821 cgatcgcctc gccgtcgcgc acgaccacgc gatgcgtgcc gcgcacctcg cgagacttcg
     2881 ccgagactcc caacctgatc gccgcgcccg ccagcgccat cgcggcctcg ttgccggggc
     2941 tcgcgacctc gagcgccgcc gaccgacccg ggtcgctgag cgtgccggcc gcaaggaagg
     3001 cgccgcgcca cacggcggcc aactcggtcg ggttgccggt ggtgagcttg ttcggcagcc
     3061 cgcgaaccgg gtgatgccga aggtcgagga ggcccgtctg gcgcgccaac gtctcgccgc
     3121 ccgcgacgac ccgcacgatg aagtgccggc tgtgcttgcc gtccccgccc gcgatcaccg
     3181 ccacgtcgct gcgcaccgcg tacacctcgg caagggtctt tcgcacccgc accgcgagct
     3241 ccgggctgtc gagctcgctt tcgacggcga tcctgcccga gatgcggtgc agcccgccgg
     3301 agaaccgcaa cgtcgccgtg agttcggcgg cacgcacccg cgtcttggtg atgggaaaga
     3361 cgacgagttc cgctttgacg tcggccgtta gtgccactct tgaggatgcc tttctcgagg
     3421 atcgttaggt gccgtgcggc cggcgcgtgt tccttgcggc cgtgtcactc ccggccgagg
     3481 tcgcgatgct gcacggagct cgtcaccgag ggcatctgcg tgagccgccg gccaagctct
     3541 tccgctatgg ccaccgaccg gtgtttgccg ccggtgcagc cgaccgccac cacgaggtgc
     3601 cgcttgttct cggcccggta gccgtcgatg acgaggcgca gaacctcatt gtacttgtcg
     3661 aggaacgggc cggccgaggc gctggcgaac acgtactgcg cgacgtcttc gtcgaggccg
     3721 gtgtggtgcc gcagctccgg cacccagaac gggttgggca agaagcgcgt gtcgaccacc
     3781 atgtcggcgt cggtcggcac gccgtatttg aacccgaaac tcatgacggt gacccgcatc
     3841 tcgtcccggt cgccggtcgc gaagagggtg gcgacgctcg tggccagttg gtggatgttg
     3901 aggtcggaag agtcgatgac gatgtcagag gcggccctga ggtcggtgag gcgcgtgcgt
     3961 tcggcggcga tgccgtcgag gagcgtgccc tggccctgca agggatgcgg gcggcgcacc
     4021 gactcgaacc ggcgcacgag ggcctcgtcg gtcgcctcca ggaaaagaat gcgcgcgcgg
     4081 gtgccgagcc gcaggctgtg cacgatgtct tcgatgtcgg cgaagaagtc ccgccctcgc
     4141 acgtcgacga ccgccgcgat cttcggcagc cgctccccgg cgcggctcgc gagttcgatg
     4201 agagggcgca gcatctgcgg cggcagattg tcgacgacgt accagccgag gtcttcgagc
     4261 gcattcgcca ccgtggagcg c
//
LOCUS       DASTNL010000220         8088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_535073, whole genome shotgun sequence.
ACCESSION   DASTNL010000220 DASTNL010000000
VERSION     DASTNL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535073"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFQ96_07050"
     CDS             <1..669
                     /locus_tag="VFQ96_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257579.1"
                     /translation="TAYRARGGTVKVLTGITQPARWPELSEERVADVVAAVRGWAGVT
                     VLDTGFSVEADEELSSDVFAPRRNAATIAALRSADRIVLVGSADPVGISRLVRSHAEL
                     GELLPGVQPTVVVNRVAGKGAAPGQAYQVRSVLERFGGIVDPVLVPDDPRAFGRVALA
                     GRGLADVAARSPARVAVRRLADVVLPPPPAPARRRRSWHVMVPFRHSWGGGPTVDWHR
                     ADAE"
     gene            653..2170
                     /locus_tag="VFQ96_07055"
     CDS             653..2170
                     /locus_tag="VFQ96_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEU0257580.1"
                     /translation="MPTLSELVLAQGVQQGAADWIHRLVRDWQLLADLAFADVVLWVP
                     TPGGSFIAVAQARPSGAATLFYRDFVGQPIRPEWHDQVTAAFETAALVDSTAPDWYEE
                     TPTNVLAVPVVRRLDPHSSEVAAVPVAVVTRHNNLGEARSVSRQETTFNACAGDIFAM
                     IAAGDFPDAGAPTGPRRGAPRASDGLIRIDEDGVTTFASPNALSAFNRIGFTGELEGE
                     SLADVTTQIVRGGGGSDGGDGEADESLPLVVTGRAPWRTDLESRGVTVALRAIPIRKN
                     GKRTGAIVLCRDVTELRHQERELITKDATIREIHHRVKNNLQTVASLLRIQARRTHSE
                     EVREALSQAQRRVGAIAVVHDTLSEGLDQVVDFDEVFARVLILVAEVAANHGTTVHPK
                     STGKFGELPSTYATPLALALTELVTNAVEHGLVGREGAVEIVAERTDEHLSVKVVDNG
                     SGLPEGKVGSGLGTQIVRTLIQGELGGSIDWHTLMGQGTEVTIEVPLRYLIAAAS"
     gene            complement(2288..2536)
                     /locus_tag="VFQ96_07060"
     CDS             complement(2288..2536)
                     /locus_tag="VFQ96_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU0257581.1"
                     /translation="MDWRDQAACLNADPELFFPVGNTGPALEQIEKAKAICARCPVTE
                     ICLQYALETGQDHGVWGGLSEDERRALKRRAARARRAS"
     gene            2724..3146
                     /locus_tag="VFQ96_07065"
     CDS             2724..3146
                     /locus_tag="VFQ96_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257582.1"
                     /translation="MTKAPEPRDAPGLTGGRAVSIPVAGLTVVLWLEVLLMAVVTVVI
                     VVDLVVQRPTSYASAVAILVLALVAGVWLAFAAIAAPRRRSWVRAAAITWQILQGAAA
                     AGAAGDSGHPVLSWLVLLLALAGLLCALTARGMRPAGT"
     gene            complement(3171..3641)
                     /gene="bcp"
                     /locus_tag="VFQ96_07070"
     CDS             complement(3171..3641)
                     /gene="bcp"
                     /locus_tag="VFQ96_07070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU0257583.1"
                     /translation="MATNTLAKGESAPEFTLPDQDGAQVALGDFAGRNLLVYFYPAAM
                     TPGCTTEACDFRDSLDALKAAGYGVVGISKDDPATLKKFQERDHLTFPLLSDPDLAVH
                     KAYGAYGEKTTYGKTSAGVIRSTFVVNEAGKIVSAQYNVKATGHVARLRKELGL"
     gene            complement(3654..4796)
                     /gene="rsgA"
                     /locus_tag="VFQ96_07075"
     CDS             complement(3654..4796)
                     /gene="rsgA"
                     /locus_tag="VFQ96_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU0257584.1"
                     /translation="MSWWDDADGPDDDEGPDESDVRVRPNPRGSRPRTKRRPAHAGAT
                     AGFVTAVNRGRYTVLLDADAGTRAGEDEPPGGKAASDPPHAAGRTVNAARGSGLRRER
                     VVTGDFVDLVGDRTGAPGSLARIVRIHPRSSLLRRSADDTDQVERIVVANADQMLIVV
                     AASRPRPRVRLVDRLLVAAYDAEIKPLLCVTKADEADPAPFLAQFRGLGLTTFASGLT
                     SLPHAALTAALAGHRTVLIGHSGVGKSTLINRLVPGALRATGQVNAVTGRGRHTSSSS
                     RALKVPSDPPGWVIDTPGVRSFGLGHVETAHVVAAFDEVAALAALCPRGCRHTPETTG
                     CRLAAALRDDTVPAATRARIDSVLRLLAALGPPGDDGGATTRQPQP"
     gene            complement(4804..6159)
                     /gene="aroA"
                     /locus_tag="VFQ96_07080"
     CDS             complement(4804..6159)
                     /gene="aroA"
                     /locus_tag="VFQ96_07080"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0257585.1"
                     /translation="MLISKYSAPDFNPYQNEDPEEPPTLWAAPHAQGPLRASLTLPGS
                     KSLTNRELVLAALADGPSTLHAPLHSRDTDGMADALRALGVTIDEVSEPGAFGKDWRV
                     TPGELVGSTTIDCGQAGTIMRFLPPVAALALGPTTIDADASARSRPMSALIDALRLAG
                     VDINDDGRGRLPFTVHGTGRLPGGEVSVDASASSQFLSALLLAGARFEQGIHVTHTGP
                     SLPSLPHIRMTVACLAAHGVAVHEPGPAQWLIEPGAIRATDTTIEPDLSNAAPFLAAA
                     LVAGGSVTIEGWPATTTQVGGQLVRFLPLFGGKVEAADGRLTATGTGRVSPVRLDLSE
                     AGELAPTLTALAALADGPSDITGIGHIRFHETDRIQALTDEINALGGQVTATPDGLHI
                     EPRALHGGVWQSHDDHRMATSGALLGLAVPGVEIDGIGSTAKTLPQFPELWTRMYAEH
                     G"
     gene            6394..7002
                     /locus_tag="VFQ96_07085"
     CDS             6394..7002
                     /locus_tag="VFQ96_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0257586.1"
                     /translation="MTAATDDLDVLFEQQALPYMDQLYGAALRMTRNRTDAQDLVQET
                     FVKGFAAFRQFKQGTNLKAWLYRILTNTFINTYRKKQREPFQGTLEDLEDWQLGGAVS
                     RTAASSTRSAEAEALDHLPDGAVKDALQEVPEDFRLAVYLADVEGFSYQEIADMMNTP
                     IGTVMSRLHRGRRLLRARLSDYARDRGYLSAAPGVHDSGSIA"
     gene            6999..7250
                     /locus_tag="VFQ96_07090"
     CDS             6999..7250
                     /locus_tag="VFQ96_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0257587.1"
                     /translation="MSDCGCQKAREDLEEYLHHELCKEDAADIREHLQSCPDCAREHR
                     VGVALTDTVRRACRDEAAPEELRAQVLTAIRTFQASHTL"
     gene            complement(7360..>8088)
                     /gene="kgd"
                     /locus_tag="VFQ96_07095"
     CDS             complement(7360..>8088)
                     /gene="kgd"
                     /locus_tag="VFQ96_07095"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188696.1"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEU0257588.1"
                     /translation="DFANGAQTVIDEFVTSAEQKWGQRSSLVLLLPHGYEGQGPDHSS
                     ARIERYLQMCAEDNLTVAQPSTPASYFHLLRRQAYSHPRKPLVVFTPKSMLRLRGATS
                     EVADFTSGRFEPIIDDHGVANKAGVRRVLLTSGKLYYDLASHLEKNPDDRIAVVRVEQ
                     YYPLPAARLTEVLAGYPNAELFWVQEEPENQGAWPYMIVETSKLGPRPLGVISRAASA
                     SPATGSAKRHAAEQNELVTRALAF"
BASE COUNT         1184 a         2725 c         2925 g         1254 t
ORIGIN      
        1 accgcctacc gggcgcgcgg cggcaccgtc aaggtgctga caggcatcac gcagccggcc
       61 cgttggccgg agctctccga ggaacgggtg gccgacgtcg tcgcggcagt gcgtggctgg
      121 gccggtgtca ccgtgctcga caccgggttc agcgtggagg ccgatgaaga gctctccagc
      181 gacgtcttcg cgccccggcg caacgcggcg acgatcgccg cgctgcgttc cgccgaccgg
      241 atcgtgctcg tcggctctgc cgatccggtg ggcatctccc gcctggtgcg ctcccacgcg
      301 gagcttggtg agctgttgcc cggcgtgcaa cccacggtcg tcgtgaaccg ggtcgccgga
      361 aagggcgctg ctcccggtca ggcctatcag gtgcgctctg tcttggaacg tttcggtggc
      421 atagtagacc cggtgctggt tcccgacgac ccgcgcgcct tcggccgggt ggctctcgcc
      481 ggccggggcc tcgccgacgt ggcggcgcgc tcgccggcgc gtgtggcggt acgtcggctg
      541 gccgacgtcg tcctcccgcc gccaccggcc ccggcgcgcc ggcgccgctc gtggcatgtg
      601 atggtgccct tcaggcactc gtggggtggc ggaccgaccg tagactggca ccgtgccgac
      661 gctgagtgaa ctcgttcttg cgcagggcgt ccagcagggt gctgcagact ggatccaccg
      721 gctggtgcga gactggcagc tcttggccga cctggcattc gccgacgtcg tgctgtgggt
      781 gccgacgcca ggcggaagtt tcatcgccgt cgcccaggcg aggccctccg gcgccgccac
      841 ccttttctat cgcgacttcg tcggccagcc gatcaggccg gaatggcacg accaggtgac
      901 ggccgcgttc gagacggccg cgctcgtcga ctccaccgca cccgactggt acgaggagac
      961 gccgacgaac gtgctcgcgg tgcccgtcgt gcgccggctc gacccgcact cctctgaggt
     1021 ggccgccgtg cccgttgccg tcgtcacccg gcataacaat cttggcgagg cccgctccgt
     1081 gagccgtcag gagaccacgt tcaatgcctg cgccggcgac atcttcgcga tgatcgcggc
     1141 cggtgacttc cccgatgcgg gcgcgcccac cgggccgcgc cgcggcgcgc cccgggcatc
     1201 cgacggtctc attcgcatcg acgaggacgg cgtcacgacg ttcgcgagcc cgaacgcgct
     1261 ttctgccttc aaccgcatcg gcttcacggg cgagctggag ggggagtcct tggccgatgt
     1321 gacgacgcag atcgtgcggg gtggcggggg gagcgatggc ggcgatggcg aggccgacga
     1381 atcgttgccc ctcgtcgtga ccggccgcgc cccctggcgt accgatctcg aatcgagggg
     1441 ggtgaccgtt gccttgcggg ccatcccgat ccgcaaaaac ggcaagcgca cgggtgcgat
     1501 cgtgctgtgc cgcgacgtca ccgagctgcg tcaccaagag cgcgagctca tcacgaaaga
     1561 tgcgacgatt cgcgagatcc accacagggt gaagaacaac ctgcagacgg tcgcctcact
     1621 tctgcgcatc caggcccgcc gcactcattc ggaggaggtt cgggaggctc tcagccaggc
     1681 gcaacggcgc gtaggcgcga tcgcggtcgt gcacgacacg ctctccgagg gcctcgacca
     1741 ggtcgtcgac ttcgatgagg tcttcgcccg cgtgctgatc ctcgtcgccg aggtggcggc
     1801 gaaccacggc acgaccgtgc atccgaaatc gacgggtaag ttcggcgagc tgccgagcac
     1861 atatgccacg ccgctggccc tggcgctgac cgaactcgtc accaatgcgg tcgaacacgg
     1921 cctcgtcggc cgggagggcg ccgtcgagat cgtcgcggaa cgcaccgacg agcacctttc
     1981 cgtcaaggtc gtcgacaacg gttccgggct gccagagggc aaggtcggtt ccgggctcgg
     2041 cacccagatc gtgcgcacct tgattcaggg cgaactgggc ggcagcatcg actggcatac
     2101 gctcatgggc cagggcacgg aggtgaccat cgaggttccg ctgcgctacc tcatcgctgc
     2161 tgcctcgtga cgtgaggggc gggtcgggcg cgacggcgct gctcgctcgg ccggcccacg
     2221 tcagagggca gggcggccgg aaggcgatcc gtcggggccc gtcgaagccc ggccccgggc
     2281 gcgaccgtca ggaggcgcga cgggcgcggg cggcgcggcg cttcagggcg cgccgctcat
     2341 cttccgaaag gccgccccag acgccgtggt cctggccggt ctccagcgcg tactgcaggc
     2401 aaatctcggt caccgggcac cgggcacaga ttgccttggc cttctcgatc tgctccagcg
     2461 ccgggcccgt gttgcctacg gggaaaaaca gctcggggtc ggcattgagg caagcggcct
     2521 gatcgcgcca gtccatggtg ctccttcggg aaggcctgtt cgcgcgagga ggcgcgcggc
     2581 ctaagatgtg gtgtggacgc cgggtccgaa cgcggcaggt ctgctgacag atgcccgccc
     2641 accaccttgt gagcaacgac tcgacggaga acatcgtcgc atagacacca cgccaaatca
     2701 atagcttcgt acaggatccc gtcgtgacca aagcccccga accccgtgac gcaccgggtc
     2761 tgaccggcgg gcgtgccgtc tcgatcccgg tggccgggct gaccgtcgtc ctctggcttg
     2821 aagtcttgct gatggccgtc gtgaccgtcg tcatcgtcgt cgacctcgtc gtgcaacgac
     2881 ccacgtcgta cgccagcgcc gtggcgatct tggtgcttgc gctcgtcgcc ggggtgtggc
     2941 tcgccttcgc cgcgatcgcg gcgccccgca ggcgttcctg ggtgcgggcc gccgcgatca
     3001 cctggcagat tctgcagggc gcggccgcag ccggggcggc cggcgacagc gggcacccgg
     3061 tcttgtcgtg gctggtgcta ctcctggcgc tcgccggcct tctctgcgcg ctgacggcgc
     3121 gcggcatgcg gcctgccgga acctgatctg acgaagcgac gaggcgcgac ctagaggccg
     3181 agctctttgc gcagccgtgc cacgtgcccg gtcgccttca cgttgtattg ggccgagacg
     3241 atcttgcccg cctcgttcac cacgaacgtc gagcggatga cgcccgccga ggtcttgccg
     3301 tacgtcgtct tctcgccgta agccccgtac gccttgtgca cggcgagatc ggggtcgctc
     3361 aagagcggaa acgtcaggtg gtcgcgctct tggaacttct tcagcgtcgc cgggtcgtcc
     3421 ttagatatcc ccaccacgcc gtagccggct gccttcaacg cgtcgaggct gtcgcgaaag
     3481 tcgcaggcct ccgtcgtgca gcccggcgtc atcgcggcgg ggtagaagta gaccaagagg
     3541 ttccgcccgg cgaagtcgcc taacgccacc tgggcgccgt cttgatcggg cagagtgaat
     3601 tctggggcgc tctcgccctt cgccaatgtg ttcgttgcca tgtcaccagg gtgtcacggt
     3661 tgtggctgtc gcgtcgtggc gcctccgtcg tcgccggggg gtccgagcgc ggcgaggagc
     3721 cgcagcaccg agtcgatccg ggcccgggtg gccgccggca ccgtgtcgtc tcggagggcc
     3781 gcggcaagcc tgcagcccgt cgtctccggc gtgtggcggc accctcgggg gcagagcgca
     3841 gcgagtgcgg cgacctcgtc gaaggccgcc acgacgtgcg cggtctcgac atgccccagg
     3901 ccgaacgaac gcacccccgg ggtgtcgatg acccagccag gcgggtcgga gggcaccttc
     3961 aaggccctcg acgaggagga ggtgtgccga ccgcggccgg tcaccgcgtt cacctgcccc
     4021 gtcgcgcgca gggcgcccgg cacgagcctg ttgatgaggg ttgacttgcc cacgccggaa
     4081 tgcccgatga gcacggtgcg atggccggcc agcgccgcgg tcagcgcggc gtgcggcagc
     4141 gacgtcaagc cgcttgcgaa cgtcgtgagg ccgaggccgc gaaattgcgc gaggaacggc
     4201 gcggggtcgg cctcgtcggc cttcgtgacg cagagcagag gcttgatctc cgcgtcgtac
     4261 gccgctacga gcagcctgtc gacgaggcgc acgcgcggcc tcggccgtga ggccgcgacg
     4321 acgatgagca tctggtcggc gttcgccacg acgatgcgtt ccacctggtc ggtgtcgtca
     4381 gcgctgcggc gcagcagact ggagcgcggg tggatgcgca cgatgcgggc gagggagccg
     4441 ggcgcgccgg tacggtcgcc gacgaggtcg acgaagtcgc ccgtgacgac ccgttcccgg
     4501 cgcagcccgc tgccgcgcgc cgcgttgacg gtgcgcccgg cggcgtgcgg cgggtcggat
     4561 gccgccttcc cgccgggcgg ttcgtcctcg cccgctctgg tacccgcgtc ggcgtcgaga
     4621 agcaccgtgt agcggccgcg gttgacggcg gtgacgaaac ccgcggtggc gccggcatgc
     4681 gcggggcgac gcttcgtgcg cggccgggag ccgcgggggt tcgggcggac ccgcacgtcg
     4741 gattcgtctg ggccctcgtc gtcgtccggc ccgtcggcgt catcccacca ggacatcggg
     4801 cccttagccg tgctccgcgt acatccgggt ccaaagctct gggaactgcg gcagcgtctt
     4861 cgccgtcgac ccgatgccgt cgatctcgac gccgggcacc gcgaggccca ggagcgcccc
     4921 gctcgtcgcc atccgatggt cgtcgtggct ctgccacacc ccgccgtgca atgcccgcgg
     4981 ctcgatgtgc agcccatccg gggtggccgt cacctggccg cccaacgcgt tgatctcgtc
     5041 ggtgagcgcc tggatacggt cggtctcgtg aaaacggatg tgcccgatgc ccgtgatgtc
     5101 gctcgggccg tcggcgagcg cggcgagcgc ggtgagtgtc ggcgcgagct cgccggcctc
     5161 gctgaggtcg agccgcacgg gggagactcg gcccgtgccg gtggccgtca gcctgccgtc
     5221 cgcggcctcg acctttccgc cgaacagcgg caggaaccgc accagctggc cgccgacctg
     5281 cgtggtcgtc gccggccagc cctcgattgt cacactgccg ccggcgacga gcgcggcggc
     5341 gaggaagggc gcggcgttag agaggtcagg ctcgatcgtg gtgtcggtgg cacggatcgc
     5401 tccgggctcg atcagccact gcgcagggcc gggttcgtgc accgcgacgc cgtgggccgc
     5461 gaggcaggcc acggtcatgc ggatatgcgg caggctcggc aggctgggcc cggtatgcgt
     5521 gacgtggatg ccctgctcga accgggcacc ggccagaagc agcgccgaga ggaactgact
     5581 ggaggccgag gcgtcgacgc tcacctcgcc gccgggcagg cgacccgtgc catgcacggt
     5641 gaacggcaac cggccgcggc cgtcgtcgtt gatgtcgacg ccggcgagcc gcaacgcgtc
     5701 gatgagcgcc gacatgggtc gggaacgcgc cgaggcgtcg gcgtcgatcg tcgtcgggcc
     5761 gagcgcgagc gcggcgacgg gcggaaggaa gcgcatgatg gtgccggcct gcccgcagtc
     5821 gatggtcgtg gagccgacca gctcgccggg ggtgacccgc caatccttgc cgaaggcgcc
     5881 tggctcgctg acctcgtcga tcgtgacgcc cagcgcgcgc aaggcgtcgg ccattccgtc
     5941 ggtgtcgcga gaatgcagcg gtgcgtgcag ggtggagggc ccgtcggcga gcgccgcgag
     6001 caccagttcc cggttggtca gcgacttcga ccccggcagc gtgaggctcg cccggagcgg
     6061 cccctgggcg tgcggagccg cccacaacgt cggcggctcc tccggatctt cgttctggta
     6121 agggttgaag tccggtgcgg aatatttgga gatcaacatg acttctccag ggtatcggtg
     6181 gcgtgcacga caacgacacc gggtcggttc ggtagacgcc ggaaacatga ggtcgaaggg
     6241 ccgcagaccg gcgggggcgg ggcggccctc ggcaggtgtc gcgggagtgt tcacggggag
     6301 gagtgcgtga tgcagacggt gttgacacgt gggccttcat gggcaccgga aacgacgcgc
     6361 cacgccgcgg cggacaccct agactgggcc gtgatgacgg ccgcgacaga cgacttggac
     6421 gtgctcttcg agcagcaggc tctgccctac atggatcagc tctatggggc ggccctgcgt
     6481 atgacgcgca accgcacgga cgcgcaagac ctggtgcagg agacgttcgt caaggggttt
     6541 gcggcgttcc ggcagttcaa acagggtacg aacctgaagg cctggctcta ccgcatcctc
     6601 accaacacct tcatcaacac gtaccgcaag aagcagcgag agcccttcca aggcaccttg
     6661 gaagacctcg aagactggca gctgggcggc gcggtctcgc gcacggccgc gtcctccacc
     6721 cgttcggcgg aggcggaggc gctcgaccat ctgcctgacg gggcggtcaa agacgcgctc
     6781 caagaggtgc cggaagattt ccgcctggcc gtgtacctgg cggatgtgga ggggttctcc
     6841 taccaggaga tcgccgacat gatgaacacc ccgatcggga cggtgatgag caggctgcat
     6901 cgtggcagac ggctcctgcg ggcccggttg tccgattatg cccgagaccg tggatacctt
     6961 tctgcggcgc cgggcgtgca cgattccggg agtatcgcat gagcgactgt ggatgccaga
     7021 aggccagaga ggatctcgag gagtacctgc accacgagtt gtgcaaagaa gacgccgccg
     7081 acattcgtga gcacttgcag agttgccccg actgcgcgcg cgagcatcgt gtcggcgtcg
     7141 cactgaccga caccgtgcgc cgggcctgcc gcgacgaggc cgcccccgaa gagttgcggg
     7201 cgcaggtctt gaccgcgatc cgcaccttcc aagccagcca cacgctgtag gggcgcggcc
     7261 ggcgccaggc gcggccggcg atcggcgggc gaagcgtcac tgctgcccgc cctcaccggc
     7321 gcgtgcgacg tcggggaggg cgggcgcgcg ctccgccggc tagaaggcga gcgcccgggt
     7381 gaccaactcg ttctgctcgg cggcgtgacg cttggcggag ccggttgcag gagaggccga
     7441 tgcggcccgg gagatgacgc ccagggggcg cgggccgagc ttcgaggtct ccacgatcat
     7501 gtacggccaa gcgccctggt tctccggctc ctcttgcacc cagaaaagct ccgcgttcgg
     7561 gtagccggcc agcacctcgg tcagccgggc cgcgggcagc gggtagtact gctccacccg
     7621 cacgacggcg atccggtcgt cggggttctt ctccaggtgg ctggcgaggt cgtaatacag
     7681 cttgccggag gtgaggagca cccgacgcac accggccttg tttgccacgc cgtggtcgtc
     7741 gatgatcggc tcgaagcggc cgctcgtgaa atcggcgacc tcgctcgtcg caccgcgcag
     7801 gcgcagcatc gacttcggcg tgaagacgac gagcggtttg cgagggtgcg aatacgcctg
     7861 tcggcgcagc aggtggaagt aggaggccgg ggtggagggc tgtgcgacgg tcaggttgtc
     7921 ttcggcacac atctggaggt aacgctcgat gcgggcggag gaatggtcgg gcccctggcc
     7981 ttcgtaaccg tgcggcaaga gcagaacgag ggaggagcgc tgcccccact tctgctcggc
     8041 cgacgtgacg aactcgtcga tcaccgtctg cgcgccgttc gcgaagtc
//
LOCUS       DASTNL010000221         5952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_906949, whole genome shotgun sequence.
ACCESSION   DASTNL010000221 DASTNL010000000
VERSION     DASTNL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906949"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFQ96_07100"
     CDS             complement(<1..154)
                     /locus_tag="VFQ96_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU0257589.1"
                     /translation="MLYWFLKYFLIGPTLRMVFRPWVVGGENLPRSGAVIMAGNHLSF
                     IDSIFHP"
     gene            complement(169..1119)
                     /locus_tag="VFQ96_07105"
     CDS             complement(169..1119)
                     /locus_tag="VFQ96_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0257590.1"
                     /translation="MYAIGIDIGGTKIGGAVVDESGAIIAQDRVPSPAAHADQMEDAV
                     AAMITRLSASYEVCAAGVAAAGFVNSKTGVIYYAPNIQWREEPFLDEMRGRVDLPLLV
                     ENDANAAGWGEFRFGAGRGVGDMVMLTIGTGVGGAIIGDGRLFRGGFGAGAELGHMRV
                     VPGGLPCGCGMRGCIEQYGSGRALQRFANDLADAGGIGSVLAARRAADGGDLSGQAIA
                     DLVAKNDPGALAAVRELGGWIGQACASLDAILDPEVFILGGGVAQLGDPLLGAVRAGF
                     LASLPARGYHPEPTFRTALLGNRAGAAGVADLARLSVQGD"
     gene            1255..3051
                     /locus_tag="VFQ96_07110"
     CDS             1255..3051
                     /locus_tag="VFQ96_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEU0257591.1"
                     /translation="MQESIVAALVRPEPERNAGDLLAERAALTPDKVLFSVPGPGGWR
                     DVTCAQFEAQVVALAKGFAAAGIGPGDKIGLMCTTRYEWILIDFAVWYAGAILVPVYE
                     TSAPAQIYRELGDSAATAMIVETAEHFARFDEVHPDLPLIGSVWQLGLGDLDKLVDRG
                     RLVPDEEIERRRHLAVGDDVATLIYTSGSTGEPKGCVITHSNLVELTKNTALAMSDVV
                     GTPGASTLLFVTAAHVFARFVAVLCVYAGVRVGHQADTSRLLPALASFRPTFLLAVPR
                     VFEKVYNSAEQKAESGGRGGVFRRAADTAVEYSTAREAGGPMPLGLRLRFRLFDLLVY
                     RRLRAALGGRARYAVSGSAPLSERLARFFDALGLTVLEGYGLTETTAPATVSPAGRVK
                     IGTVGTPLPGTGVRIADDGEILLRGVNIFRGYWNDPESTTRSFDGEWFKTGDIGSLDE
                     DGYLSVTGRKKEIIVTAGGKNVAPAGLESVIGANPLVDQVLVVGDRRPFVAALVTLDA
                     EMLPLWLNDHREDPGMSLAQAAVNPAVRAEIQRAVDAANSRVSRAESVRKFVVLDTQW
                     TQESGHLTPKMSVKRDVVLHDFQATLDGLYSA"
     gene            complement(3087..3578)
                     /locus_tag="VFQ96_07115"
     CDS             complement(3087..3578)
                     /locus_tag="VFQ96_07115"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA];
                     GO_process: GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA];
                     GO_process: GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0257592.1"
                     /translation="MAERAIRLFGDPVLKTPSSAITSVDDKVRTLIADLLDTVRLPGR
                     AGVAAAQIGVGLCAFSYNVDGQVGYLINPRIVELGGEPEPMEEGCLSVPGLWFPTLRH
                     PSATARGTDLDGAEVEVSGTGILAQALQHETDHLAGMLYLDRLDKEHRREAMRQIRAS
                     DWF"
     gene            complement(3579..5006)
                     /locus_tag="VFQ96_07120"
     CDS             complement(3579..5006)
                     /locus_tag="VFQ96_07120"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HEU0257593.1"
                     /translation="MSESSAVVDYDLLVIGAGSGNTLIGPEFDEWSIALVDDGEHFGG
                     TCLNAGCIPTKMFVHVADVAGDAADAARLGWHQPAGHVDWPALRQRIFGRTDAISAAG
                     ERYRATECPNVTLVRESVVFEDAHTVVTAGGRRLRGRVVVVAAGSRPRPLTAPHDDDP
                     AIYDSDSIMRIDQLPASMIIAGGGAVAMEFAHVFSSFGVRVTIVNRSPRLLRTLDDEV
                     SRAFTDIATRRWHVHAGSPIVRVARDGAQLDVVTADGAHHLAESLLVAQGRIPNTDRL
                     GLAPFFDVVDGRLSVDDAQRAQHDGAVVPGVYALGDICSAYELKHVANHQARLVRHNI
                     THPDAPHRGNPGPVPAAIFSHPQVAHFGLTEAEALRRYGRDHVVTARYDYAGTAYGWA
                     FEDTTGFCKLVVSAPDGAILGAHLIGPQASILLQPLIQAAALGRGVRGLARAQYWPHP
                     AAVEVVENALLHAEEALGEAADGEG"
     gene            complement(4999..>5952)
                     /locus_tag="VFQ96_07125"
     CDS             complement(4999..>5952)
                     /locus_tag="VFQ96_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEU0257594.1"
                     /translation="GEDAESTSMLTTERLLDGGRAVWPAPEGGWNRFLYSATFHRVNR
                     GDSAKVRARKELTARIQRALPGGARSVPVLTRKGGVGKTTVTTLLGMALADAREDRII
                     AIDANPDRGTLSERVPKQTRATVRDVVAKAATIGSFTDFSALVSRDVTRLDILASDTD
                     PTISEAFDENDYNVVADLASRYYSVILTDCGTGIVHSVMRATLRRADSVVIVSGGSVD
                     EARLASETLTWLETNGYGELAANSVVALNTATQGTNLVHVDELESHFLSRVRDVVRIP
                     YDPQLAAGSHVSYKDLRPLTRRSARVLAALVVEGLPESRDE"
BASE COUNT          891 a         2043 c         2082 g          936 t
ORIGIN      
        1 gcggatggaa gatcgagtcg atgaacgaga gatggttgcc tgccatgatc acggccccgg
       61 aacgcggcag gttttcgccc cccaccaccc agggtcggaa caccatgcgc agcgtgggcc
      121 cgatgaggaa gtacttcagg aaccagtaga gcatcgcccg cctcgccctt agtcgccctg
      181 cacgctgagg cgggcgaggt cggccacgcc cgccgcgccg gcccggttgc cgagcagcgc
      241 ggtgcggaac gtcggttccg ggtgatagcc ccgggccggc aggctcgcga ggaacccggc
      301 gcgcaccgcg ccgagaagag ggtcgcccag ctgcgcgacg ccgccaccca agatgaagac
      361 ctcggggtcg aggatcgcgt cgaggctggc gcacgcctgg ccgatccacc cccccagctc
      421 tcgcaccgcc gccaacgccc ccgggtcgtt cttggccaca aggtcggcga tggcctggcc
      481 gctcaagtcg ccgccgtccg ccgcacggcg tgcggcgagg accgagccga tcccccccgc
      541 gtcggccagg tcattcgcaa aacgctgcag ggcacggccc gagccgtatt gctcgataca
      601 gccgcgcatg ccgcagccgc agggcagccc gcccggaacg acccgcatgt ggccaagttc
      661 cgcgccggcg ccgaagccgc cgcggaagag gcgcccgtcg ccgatgatcg cgccgccgac
      721 gcccgtgccg atggtcaaca tcaccatgtc gccgacgccg cggccggccc cgaaccggaa
      781 ctctccccag ccggcggcgt tcgcgtcgtt ctcgacgagg agcggtaggt cgacgcgccc
      841 gcgcatctcg tcgaggaagg gttcttcgcg ccactggatg ttcggcgcgt aatagatgac
      901 gcccgtcttc gaattgacga agccggccgc ggcgaccccg gccgcacaca cctcatatga
      961 ggcgctcagc cgcgtgatca tcgcggccac cgcgtcttcc atctggtcgg cgtgcgccgc
     1021 cggagagggg accctgtctt gggcgatgat cgcacccgat tcgtcgacga cggcgccgcc
     1081 gatcttcgtc ccgccgatgt cgattccgat cgcgtacacc gatttccgac ccctccctcg
     1141 cccggtccgc cggccggcgg ctcgagggcg agcacgctct agagtgtagt taatcgcttc
     1201 tgcaccggtg taccggggca cgatgtgacc gtttcggttg ccgaaggaga tcccgtgcag
     1261 gaatcgatcg tcgccgcgct ggtccgcccg gaaccggagc gcaacgccgg cgacctcctc
     1321 gcagaacgcg cagcgctcac ccccgacaag gtgctcttct cggtccctgg ccccggcggc
     1381 tggcgcgacg tgacgtgtgc gcagttcgaa gcacaggtgg tcgccctggc gaaggggttc
     1441 gctgccgccg gcatcgggcc gggcgacaag atcggcctca tgtgcacgac tcgctatgag
     1501 tggatcttga tcgacttcgc cgtctggtat gcgggcgcga tcctcgtgcc cgtctacgag
     1561 acctccgccc ccgcacagat ctaccgggaa ctcggcgatt ccgcggctac cgcgatgatc
     1621 gtcgagaccg ccgagcattt cgcgcggttc gacgaggttc atcccgatct gccgctcatc
     1681 ggctcggtat ggcagttggg cctgggcgac ctcgacaagc tggtcgatcg cggccgcttg
     1741 gtgcctgacg aggagatcga gcgtcgccgc cacctcgcgg tcggcgacga cgtcgcgacc
     1801 ctcatctaca cttctggttc gacgggtgag ccgaagggct gcgtcataac ccacagcaac
     1861 ctcgtcgaac tgacgaagaa cacggcgctg gcgatgtctg acgtggtcgg cacgccgggt
     1921 gcctcgacac tgctcttcgt caccgccgcg catgtcttcg cgcggttcgt ggccgtcctg
     1981 tgcgtgtatg cgggagtgcg ggtggggcac caggccgaca cgtcgcgtct gctgcccgcg
     2041 ctcgcctcgt tccggccgac ctttcttctt gccgtgccgc gggtgttcga gaaggtctac
     2101 aactcggccg agcagaaggc ggagtcgggc gggcgcggcg gcgtgttccg gcgggccgcc
     2161 gatacggccg tcgaatactc caccgcgcgc gaggccggcg ggccgatgcc tctcgggctg
     2221 cgcctgcggt ttcgtctctt cgacctgctc gtctaccgac ggctccgcgc cgcgctcggc
     2281 gggcgggctc gatacgcggt ctcgggctcc gcgccgctca gcgagcgtct ggcgcgcttc
     2341 ttcgacgccc tcggtctcac ggtgttggag ggctacggct tgacggagac gacggcgccc
     2401 gcgaccgtga gcccggccgg gcgggtcaag atcggtaccg tgggcacgcc gctgccgggc
     2461 accggggtgc ggatcgccga cgacggcgag atcctccttc gcggcgtgaa catttttcgt
     2521 ggttattgga acgacccaga atctacgacg cgctccttcg acggggaatg gttcaagacc
     2581 ggcgacatcg gttccctcga cgaagacggc tacctgagcg ttacgggtcg caagaaagag
     2641 atcatcgtga cggcgggcgg caagaacgtc gcgcccgccg ggctggagag tgtgatcggt
     2701 gcgaatccgc tcgtcgacca ggtcttggtc gtcggcgacc gccggccgtt cgtcgcggct
     2761 ctcgtgaccc tcgacgccga gatgctgccc ctctggctga acgaccaccg ggaggatccg
     2821 ggcatgtcgc tcgcccaggc ggccgtcaac ccagcggtgc gggcggagat ccagcgcgcc
     2881 gtcgacgccg caaactcccg ggtttcccgg gcggagtcgg tgcgtaaatt cgtggtgctc
     2941 gacactcagt ggacccagga gagtggccat ctgacaccga agatgagcgt caagcgcgat
     3001 gtcgtgttgc atgactttca ggcgacgctt gacggtctgt attccgccta gcgtcgggcc
     3061 tccggccggc ccctcggccc gtgggatcag aaccagtcgc tcgcgcggat ctgccgcatg
     3121 gcctcacggc gatgttcctt gtcgagccgg tccaggtaga gcatgccggc caagtgatcg
     3181 gtctcgtgct gcaacgcctg cgcgaggatg ccggtgcccg agacctctac ctcggcgccg
     3241 tcgaggtcgg tgccgcgtgc cgtcgccgac gggtgacgca gggtcgggaa ccagaggccc
     3301 ggaaccgaca ggcagccctc ttccatcggc tccggctccc cgcccagctc gacgatgcgc
     3361 gggttgatga gatagccgac ctggccgtcg acgttgtagc tgaacgcgca cagcccgacg
     3421 ccgatctgcg cggccgcgac gccggctcgg ccgggcaggc gcaccgtgtc gagaaggtcg
     3481 gcaatcaacg ttcgcacttt gtcgtcgaca ctcgtgatcg cgctcgacgg ggtcttcagc
     3541 accgggtcgc cgaagagccg gatggcacgt tcggccactc agccctcccc gtcggcggcc
     3601 tcgcccaagg cctcttcggc gtgcaacagc gcgttctcga ccacctcgac cgccgccggg
     3661 tgcggccagt actgggcacg ggcgaggccg cgcacgccac ggccgagtgc cgccgcctgg
     3721 atgagcggct gcaacaggat gctcgcctgg gggccgatca ggtgggcccc gaggatcgca
     3781 ccgtcgggcg cggagacgac cagcttgcag aacccggtcg tgtcttcgaa tgcccagccg
     3841 tacgcggtgc ctgcgtagtc gtatcgcgcc gtgaccacgt ggtcccggcc gtatcgccgc
     3901 agcgcctccg cctcggtcag cccgaagtgg gcgacctgcg gatggctgaa gatggcggcc
     3961 ggcaccgggc cggggttgcc ccggtgcggg gcgtcgggat gggtgatgtt gtgccgcacc
     4021 agacgggcct gatgattggc gacgtgcttc aactcgtacg cgctgcagat gtcgccgagg
     4081 gcgtagacgc ccggcacgac ggcgccgtcg tgttgggccc gctgcgcgtc gtcgaccgac
     4141 agccggccgt cgacgacgtc gaagaacggc gccaagccca gccggtcggt gttcgggatg
     4201 cggccctggg cgacgaggag gctctcggcc aggtggtgtg cgccgtccgc cgtcacgacg
     4261 tcgagttgcg caccgtcgcg cgcgacccgc acgatcggtg agccggcatg aacgtgccaa
     4321 cgccttgtcg cgatgtcggt gaaggcccgg gagacctcgt cgtccagtgt gcgcaagagt
     4381 ctgggcgacc ggttgacgat cgtcacacgc acgccgaacg acgagaagac gtgggcgaac
     4441 tccatggcga cggcgccgcc gcccgcgatg atcatcgagg cgggcaactg gtcgatgcgc
     4501 atgatcgagt cggagtcgta gattgccgga tcgtcgtcgt gtggcgcggt gagcgggcgc
     4561 ggccgggagc cggccgcgac gacgacgacg cggccgcgca ggcgtctgcc cccggccgtc
     4621 acgaccgtat gcgcgtcttc gaagacgacg ctctcgcgca cgagcgtcac gttcgggcat
     4681 tctgtggcac gataccgctc ccccgccgcc gagatggcgt cggtgcggcc gaagatgcgc
     4741 tgccgcagag ccggccaatc gacgtgaccg gcgggctgat gccaacccag ccgggcggcg
     4801 tctgccgcat cgcctgccac atcggcgacg tgcacgaaca tcttcgtcgg tatgcatccg
     4861 gcgttcagac aggtgccgcc gaagtgctcc ccgtcgtcga cgagggcgat cgaccattcg
     4921 tcgaactcgg ggccgatcag cgtgttcccg ctgcccgcgc cgatcaccag caggtcgtag
     4981 tcgacgacgg cggaagactc actcatcgcg agactccggc agcccttcga cgacgagcgc
     5041 cgcgaggacg cgtgccgagc gcctggtcag gggccggagg tctttgtacg agacgtgcga
     5101 tccggcggca agctgcgggt cgtacggaat gcggaccaca tcgcgcacac gggaaaggaa
     5161 atgcgactcc agctcatcga cgtggacgag gttggtgccc tgggtggccg tgttgagggc
     5221 gacgacgctg ttcgccgcaa gctcgccgta gccgttcgtc tccagccagg tgagggtctc
     5281 ggaagcgagc ctcgcctcgt cgacgctgcc tccggagacg atgacgaccg aatcggcccg
     5341 ccggagcgtc gcacgcatca cggaatgcac gatgccggta ccgcagtcgg tgaggatcac
     5401 cgaatagtag cgggaggcga ggtcggcgac gacgttgtag tcgttctcgt cgaacgcctc
     5461 gctgatggtc gggtcggtgt cggaggcgag gatgtcgagt cgcgtcacgt cgcgggagac
     5521 gagggcggag aagtcggtga agctgccgat cgtggctgcc ttcgcgacca cgtcgcgcac
     5581 cgtcgcccgc gtctgcttcg gcacgcgctc cgacagggtg ccgcggtcgg ggttcgcgtc
     5641 gatcgcgatg atccgatctt cgcgcgcgtc ggcgagagcc atgccgagca gggtcgtcac
     5701 cgtggtcttg ccgacgccac ccttgcgggt gagcaccggc accgagcggg cgccgcccgg
     5761 cagcgcccgc tggatgcggg ccgtcagctc tttgcgggca cgcaccttgg ccgagtcgcc
     5821 gcggttcacg cggtggaacg tcgcagaata gaggaatcgg ttccagccgc cctccggcgc
     5881 cggccagacg gcgcgcccgc cgtcgagtag gcgctccgtc gtgagcatcg acgtggactc
     5941 ggcgtcttcg cc
//
LOCUS       DASTNL010000222         7796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_907118, whole genome shotgun sequence.
ACCESSION   DASTNL010000222 DASTNL010000000
VERSION     DASTNL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907118"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFQ96_07130"
     CDS             complement(<1..606)
                     /locus_tag="VFQ96_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEU0257595.1"
                     /translation="MTEAKRLSYATLGEARRGPAVRYPTKAAQGSTVGIVHFGLGAFY
                     RALQSVYTGEAAAETGERQWGILGVTGRTDRVARQLHPQDGLYGVLVRGASKTRLQVV
                     SAVRDVVWPGDAAERILDTIAAPTTHIITLTITEKGYHRAASGGVDTGAPAILAATHQ
                     LREELSAVGPGPRRGDVAEGPAPVPIGLLARGLARRFRRGGS"
     gene            complement(603..1685)
                     /locus_tag="VFQ96_07135"
     CDS             complement(603..1685)
                     /locus_tag="VFQ96_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257596.1"
                     /translation="MGATVRGIARRSGVSISTVSRALSGSPLVAAETSERVWAAAEEL
                     GYTPSRRPRATRERRGVLGVVVPDLGNPFFGAICKGIQERARANGYLTFVADSGEDPT
                     EEGEILRGLHGHADGVILCSPRVSDRQILQAAAETHLVLANRHSGAVPSVVFDNRAGM
                     RSAVQHLVALGHRTIGYVGGPATSWSNTQRAEAFAGFGGERAPHGLELLDLGTFPPYF
                     SGGVQAADVVVAAGVTAAVAYNDLMALGLVDRLHERGLRVPADISVVGIDGAAAATFV
                     RPNLTTVELPCLRMGRLAVDVLVGALRAAGPTAGAGRAEAAAPAEEVLATRLLVRQST
                     GVPPGGAASVADARRERDEMRGEKTA"
     gene            2110..3540
                     /gene="uxaC"
                     /locus_tag="VFQ96_07140"
     CDS             2110..3540
                     /gene="uxaC"
                     /locus_tag="VFQ96_07140"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEU0257597.1"
                     /translation="MAETPWRLDPDRALPAEPSIRPIARRIYDAVATLPLISMHGHVP
                     VENIRRNAPFGDPAELFIIPDHYVRRMLVSQGYTNEELGVASTAAGAPIETDHRAIWR
                     RFCENWHLFRGTPSRYWLEQELVEVFGVTSRPSATSADAIYDQLTKTLARPEYRIRPL
                     FDRFHIEILSTTDSPASDLADHAALRAEGWGERVVPTFRPDALFYPDRAGWPGRVDEL
                     SAAAGIDVTGYASFLAALRQRREAFAAAGARASDHGHESADTTPLDDADAERIFTAGL
                     RGTADARMAAAFAANMLYQSAKMSLDDGLVMQLHAGVLRDYSSAVYRALGPDQGFDIP
                     ARTEYTRALRPLLDDFGLDPRLRVILFTVDETVFSRELAPLAGVFPSVRLGVPWWFLD
                     SPAGMSRFRAAITETAGFYNTSGFVDDTRAFASIPARHDLARRIDAGYLARLVAEGHL
                     DLDEAVETARDLHETLPKKAYEPRGR"
     gene            3608..4936
                     /locus_tag="VFQ96_07145"
     CDS             3608..4936
                     /locus_tag="VFQ96_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0257598.1"
                     /translation="MAHLAQAITTDVRFPTSLQHDGSDAVNVDPDHSAAYLELHTDDP
                     SWPTGYAFTFTGGRGNDLMCRAIDEVVPLLPDRPLEWTVENLGEVSRALVHDAQLRWL
                     GPEKGVTHMAAGAVLNALWDIKARRAGLPLWRLLAQMSPEELVATIDFSHIRDAVTEE
                     QALQIFRDGQEGKAARIAELEEAGYPAYSTAPGWLGYDDEKMQRLAREAVSAGFAMIK
                     MKVGGSVEEDRHRLRLAREAVGPDVPIAIDANQRWDADEAAAWINALAEFHLHWVEEP
                     TSPDDVLGHARIRQEIAPTAVATGEAVQNRIVVKQLLQAHAIDYLQIDATRVAGVGEN
                     LANLLLAARFGVPVCPHAGGVGLCELVQHYSLFDYAVVSRSMAGRMTEYVDHLHEHMA
                     TPVTLRAGHYMPPTTPGTGAQIKPQSVDEWRYPDGPGWRNLIETGRVPRP"
     gene            complement(5031..5444)
                     /locus_tag="VFQ96_07150"
     CDS             complement(5031..5444)
                     /locus_tag="VFQ96_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEU0257599.1"
                     /translation="MPPRVVIDPNVWISGLINPSGTPARVIAAAAAGGFSVVISRRLL
                     DELATVLARPKFRRWITLSDAIAFIDALEREAELRPDPVAVPRRIRDVDDDYLAALAE
                     ANKATIVTGDDDLLTAGLQPPAVTPRHLLDSLAQV"
     gene            complement(5454..5714)
                     /locus_tag="VFQ96_07155"
     CDS             complement(5454..5714)
                     /locus_tag="VFQ96_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257600.1"
                     /translation="MARKKTTVYIDEDLLRAAKMTAARTGRREYEVFETALREHLGLA
                     GTIERIWAGIDPAHAPGDKEADRIASDEIAAVRATSAGRRAG"
     gene            5848..6849
                     /locus_tag="VFQ96_07160"
     CDS             5848..6849
                     /locus_tag="VFQ96_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0257601.1"
                     /translation="MATIQDVARRAGVSRSTVSYALSGKRRISSETKARIDDAIRELA
                     FTPNAGARALATAQTSVIGLFVHFFDDEFSPAMLQYVLPISDAAREYGYDVLMITDTD
                     GPKALKRVSNSDMVDGVILLNVAHEDPRLPVMRTIRQPGSMVGLPKSTDGFDVFDLDF
                     EQSARMVIDHLAERGHRDVTLVTGHKAVFERGGAYAWRFLDTAKERAARYGMRIHALY
                     GQAQQPAVNRQLAEILDGHPGTALVVHNDAVIAALPSVLHSRGVRVPDDLSVISIFSE
                     DFGRIFNLPYTAIESSPDRLGRLAVRALVQHMRDPASAGPHRVELLAPRMVDRGSTR"
     gene            6970..>7796
                     /locus_tag="VFQ96_07165"
     CDS             6970..>7796
                     /locus_tag="VFQ96_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0257602.1"
                     /translation="MHAQHTARAIGAALLTAGVVAATLTGCSSTTNTSGSKTASSGSS
                     TYTFWDPYPQYDASSDWGKLVAQCGTKAGVTVKRTGYDTSDLTNKALLAGQQGNSPDV
                     LLVDNPVVSTLASAGLLTDTADNGLSTGKIAQNILGAGVIDGKTYGVPIGANTLALYY
                     NPKILKAANVDPASITNWSTLTSALAKVTASGKKGITFAGIGTEEGSFQFLPWFWGAG
                     ANLTKLDSPQANAALSLWTNWIKKGYAPNSVIQDTQTTSWQEFQTGDFAFGENGTWQ"
BASE COUNT         1347 a         2798 c         2490 g         1161 t
ORIGIN      
        1 cgacccgccg cggcggaacc gccgggcgag gccccgggcg agcaggccga tcggaacagg
       61 tgccggcccc tctgcgacgt caccccgcct ggggccgggg cccacagccg agagttcctc
      121 tctcagctgg tgcgtggcag cgaggatcgc cggggcaccc gtgtcgacgc cgcccgacgc
      181 ggcccggtga taccccttct cagtgatcgt cagggtgatg atatgcgtcg tcggcgccgc
      241 gatcgtgtcg aggatccgtt cggcggcgtc gcccggccag accacgtcac gcacggcgga
      301 aaccacctgc aggcgggtct tcgatgcgcc gcgcacgagc accccataga ggccgtcttg
      361 cgggtgcagc tggcgtgcca cacggtcggt gcggccggtg acgccgagga tcccccactg
      421 gcgctcgccc gtctcggcag cggcctcgcc ggtgtagacg ctctgcaacg cccggtagaa
      481 ggcgccgagg ccgaaatgca cgatgccgac cgtcgagcct tgcgcggcct tggtggggta
      541 tcgcaccgcc gggccacggc gtgcctcgcc caacgtcgca tacgaaagcc gcttcgcctc
      601 ggtcatgccg tcttctcgcc tctcatctcg tccctttccc gccgggcatc cgccacactt
      661 gccgcgccgc ccggcgggac accggtcgac tgtcgcacaa ggagccgcgt ggcaagcacc
      721 tcttccgcag gcgccgccgc ctcggcgcgg ccggcaccgg ccgtagggcc cgccgcgcgt
      781 aacgcaccca ccagcacgtc gaccgcgagc cggcccatcc gcaggcacgg caactcgacg
      841 gtcgtgaggt tcggccgcac gaacgtcgct gccgcggccc cgtcgatgcc gaccacgctg
      901 atgtcggcgg gcacgcgcag gccgcgctcg tggagccggt cgacgaggcc caacgccatg
      961 aggtcgttgt aggcgacggc cgcggtcaca ccggccgcca ccacgacatc cgccgcctgc
     1021 acgccgccgg agaagtatgg cggaaaagtg ccaaggtcga gaagctccag gccgtggggc
     1081 gcccgctcgc cgccgaaacc cgcgaaggcc tcggcccgtt gcgtgttgga ccacgacgtc
     1141 gcgggcccac cgacgtagcc gatcgtacgg tggccgagcg cgacaaggtg ctgcaccgcc
     1201 gaacgcatgc cggcccgatt gtcgaagacc accgagggca cggcacccga gtgcctattg
     1261 gcgagcacca ggtgggtctc ggcggccgcc tgcaggatct ggcggtcgga gacgcgcggc
     1321 gagcagagga tgacgccgtc ggcgtgaccg tgaagacccc gcagaatctc tccctcttcc
     1381 gtcgggtctt ctccgctgtc ggccacgaag gtgagatagc cgttcgcgcg cgcccgctct
     1441 tgaattccct tgcagatcgc cccgaagaac gggttgccga ggtcaggcac gacgacgccc
     1501 agcaccccgc gacgctccct cgtcgcgcgc ggccgacggc tgggcgtgta acccaactcc
     1561 tccgcggcag cccacacccg ctcactcgtc tccgccgcga cgagcgggga gcccgacaag
     1621 gcccgcgaga cggtcgagat ggagaccccg ctgcgacgcg cgatgccgcg cacggtcgca
     1681 cccatgccac acctccgata cgcccgtgca cgatcacgcg gcgcgacggc ctgcgaacct
     1741 catgacgccg gcccggctgg tggcgtgcgc gagacccggc ggccgcaagg cccgtcgcaa
     1801 tccccatccc gtggccgacg tagggccggt cacgtcttca gtatgccgac aatcgaacac
     1861 ctcgtcaaag tgtttcacga aacaaacgag ggacggcaac gccctgggat tgaagtcgac
     1921 accttgttgc gcctatcgta tcgtcatagg gcggctggca aaacgtacgg aagatacatg
     1981 tttttgagcg gcccgtccag gtggtgcatg tctgcgcggc agggtcgctc tcagaaagag
     2041 ccgctcgcca gccacagacc ctgcacccaa ttgtctcatt ttgtttcatc atgttgaaga
     2101 ggggcataca tggccgaaac accctggcgg ctcgacccgg atcgcgcttt gccggccgaa
     2161 ccgtcgattc ggccgatcgc acgccgtatc tacgacgcgg tcgcgactct cccgctcatc
     2221 tcgatgcacg gccacgtgcc ggtcgagaac attcgccgca acgcgccgtt cggcgacccg
     2281 gccgagctgt tcatcatccc cgaccattac gtgcggcgga tgctcgtctc gcagggctac
     2341 acgaacgaag agctgggggt cgcctccacc gccgccggcg caccgatcga gacggatcat
     2401 cgcgcgatct ggcgccgatt ctgcgagaac tggcacctct ttcgcggcac cccgagccgc
     2461 tattggctgg agcaggagct cgtcgaggtg ttcggcgtga cgagccggcc ctccgcaacg
     2521 agcgccgacg cgatctacga ccagctcacc aagacgctgg cgaggcccga atatcgcatc
     2581 cgcccgctct tcgaccgctt ccacatcgag atcctctcga cgacggattc gccggccagc
     2641 gacctcgcag atcacgcggc gttgcgggcc gaagggtggg gtgagcgggt cgtgccgacc
     2701 tttcgcccgg acgcgttgtt ctaccccgac cgagcgggtt ggccgggccg tgtcgacgag
     2761 ctctcggcgg cggccggtat cgacgtcacc ggctacgcct ctttcctcgc agcgctgcgg
     2821 cagcggcggg aagcgttcgc ggcggccggc gcccgagcca gcgaccacgg gcacgagtcc
     2881 gccgacacga cccccctgga cgacgccgac gccgagcgga tcttcacggc cgggctgcgc
     2941 ggcaccgccg acgcgcgcat ggcggccgca ttcgccgcga acatgctcta ccagagcgcc
     3001 aagatgtcac tcgacgacgg cctcgtcatg cagctgcatg ccggggtgct gcgggattac
     3061 tcgtcggccg tgtaccgcgc gctcggcccc gatcaaggtt tcgacatccc cgcccgcacg
     3121 gagtacacgc gcgcgctgcg cccgctcctc gacgacttcg gcctcgaccc gcgtctgcga
     3181 gtgatcctct tcacggttga cgagacggtt ttctcgcgcg agctggcgcc cctcgccgga
     3241 gtcttcccct cggtacggct cggcgtaccg tggtggttcc tcgactcgcc ggccggcatg
     3301 tcgcgcttcc gggccgcgat caccgagacg gccggcttct acaacacgag cggattcgtc
     3361 gacgacacgc gagcgttcgc ctccatcccc gcccgccacg acttggcccg gcgcatcgat
     3421 gccggctatc tggcccggct cgtcgccgag ggccacctcg acctcgatga ggccgtcgag
     3481 acggcacgcg acctgcacga gacgctaccc aagaaggcgt acgagccgcg tggccgctga
     3541 acgcaggccc gcgcagagga cgaaacagac gcgacccgca cggtaagaca cgagcgaagg
     3601 agaatccatg gcccacctgg cccaggcgat cacgacggac gtgcggttcc cgacgtcgtt
     3661 gcagcacgac ggttccgacg cggtgaacgt cgaccccgac cactccgccg cctacctgga
     3721 attgcacacc gacgacccgt cgtggcccac cggctacgcc ttcaccttca ccggcggccg
     3781 gggcaacgac ctgatgtgcc gcgccatcga cgaggtcgta ccgctcttgc ccgacaggcc
     3841 gctcgaatgg acggtcgaga acctgggcga ggtctcccgc gccctcgtgc acgacgcgca
     3901 actacgctgg ctcggccccg agaaaggtgt cacgcatatg gcggccggcg cggtactgaa
     3961 cgcgctgtgg gacatcaagg ccaggcgggc ggggctgccg ctctggcgcc tcctggcgca
     4021 gatgtcgccg gaggagctgg tggcgacgat cgacttcagc cacatccgcg acgccgtcac
     4081 cgaagagcag gccctgcaga tcttccgcga cggccaagag ggcaaggccg cccggatcgc
     4141 ggagctggag gaagccggat acccggccta ctcgaccgcc cccggctggc tcggctacga
     4201 cgacgagaag atgcagcgcc tcgcgcgcga ggccgtttcg gccgggttcg ccatgatcaa
     4261 gatgaaggtg ggcggcagcg tcgaagaaga ccggcaccgt ctgcggctcg cccgcgaggc
     4321 agtggggccc gacgttccga tcgcgatcga cgcgaaccag cggtgggacg ccgacgaggc
     4381 cgcggcctgg atcaacgccc ttgccgagtt ccacctgcac tgggtggagg agccgacgag
     4441 ccccgacgac gtgctcggcc acgcgcgcat ccgccaagag atcgcgccga ccgccgtcgc
     4501 gacgggcgag gcggtgcaga accgcatcgt cgtgaagcag cttctgcagg cccacgcgat
     4561 cgactacctg cagatcgacg ccacccgggt ggcgggcgtc ggcgagaacc tcgctaacct
     4621 cctcctcgcg gcccgcttcg gcgtgccggt ctgcccgcac gccggcggtg tggggctgtg
     4681 cgaactggtg cagcactatt cgctcttcga ctacgcggtc gtctcccgca gcatggcggg
     4741 gcgtatgacg gaatacgtcg accaccttca cgagcacatg gcgacccccg tcacgctgcg
     4801 cgccggccac tacatgccgc cgacgacacc gggcaccggg gcccagatca agccccagtc
     4861 ggttgacgag tggcgctacc ccgacgggcc cggctggcgg aacctgatcg agacggggcg
     4921 ggtgccccgg ccgtaagcag cgtcgcgacg ttgccggccc cgcaacccgg ctcgcctgcc
     4981 gcaccgattc tgtgcccgca taccaaccgg catacgtttc cagcctgtcg tcagacctgt
     5041 gcgagggaat cgaggagatg ccggggcgtg accgcgggcg gttgaagccc cgcagtgagg
     5101 aggtcatcgt cgcccgtgac gatcgtcgcc ttattcgcct cggctagggc tgccaggtag
     5161 tcgtcgtcga cgtcccgaat ccgtcgcggt acggcgacgg ggtcggggcg caattccgcc
     5221 tcgcgctcca gagcatcgat gaacgcgata gcgtcgctca gggtgatcca gcggcggaac
     5281 tttggccggg cgagcactgt ggccagctcg tccagaagcc gtcggctgat cacgacggaa
     5341 aacccgcccg cagcggctgc tgcgatcact cgcgctggtg ttccgctcgg gttgatcaga
     5401 ccggatatcc acacgttcgg atcgatgacg acccgcggcg gcacagccag cgctcagccc
     5461 gcccggcggc cggccgaagt ggcgcgcacg gcggcgattt cgtcggaggc aatgcgatcg
     5521 gcctctttgt cgcccggggc gtgggccgga tcgataccgg cccagatgcg ctcgatggtt
     5581 ccggccagac cgaggtgctc gcgcagggcg gtctcgaaca cctcatactc ccgccgaccg
     5641 gttcgagctg cggtcatctt cgccgcccgc agcaggtcct catcgatgta gaccgtggtc
     5701 ttctttcgcg ccatcaatcc attttgacag aaagactgga acactgctat cggagtacgc
     5761 ctcgtagtta ggccctgcgc cggttgacct gtcgccggag ggctgttaca gttccgatcg
     5821 aaccggttcg acgaagacag gactccgatg gcaactatcc aggacgtggc acgacgtgcc
     5881 ggcgtctcac ggagcacggt ctcgtatgcc ctatccggca aacgtcgaat ctcgagcgag
     5941 accaaggcgc gcatcgacga cgcgatccgc gaactcgctt tcacgccgaa cgcgggcgca
     6001 cgggcgctcg ctaccgcaca aacgtctgtg atcggcctgt tcgtgcactt cttcgacgac
     6061 gagttctcgc cggcgatgct gcaatacgtg ctccccatct ctgacgccgc ccgagaatac
     6121 ggctacgacg tcttgatgat caccgacacc gacgggccca aggcattgaa acgcgtctcg
     6181 aactccgaca tggtcgacgg cgtgatcctg ctgaacgtgg cgcacgagga tccgcgactg
     6241 cccgtcatgc gcaccatccg ccagccaggc tcgatggtcg gcctgcccaa gtccaccgac
     6301 ggcttcgacg tgttcgacct cgatttcgag cagtctgcac gcatggtcat cgaccatctc
     6361 gccgagcgcg ggcaccgcga tgtgaccctg gtgaccggcc acaaggccgt tttcgagcgt
     6421 ggcggggctt acgcatggcg ctttctcgac acggcgaaag aacgtgccgc gcgctatggc
     6481 atgcgcatac acgcactcta tggccaggcc cagcagccgg cggtcaaccg gcagctggcg
     6541 gagatcttgg acggacaccc cggcacggcg ctcgtcgtgc acaacgacgc ggtcatcgcg
     6601 gccctcccct ccgttttgca ttcgcgtggg gtcagggtgc ccgacgacct gtcggtcatc
     6661 agcatttttt ctgaagactt cggccgaatc ttcaacctgc cgtataccgc catcgagagt
     6721 tcgcccgacc ggcttggccg cctcgccgtg cgcgccctcg tgcaacacat gcgcgaccca
     6781 gccagcgcgg gaccccaccg cgtcgaactg ctcgcacccc gaatggtcga ccgaggcagc
     6841 acccgatgac cccacccaca aggtaaagcc acgcctctat gtcgaaccgg ttcgattgac
     6901 gttcgcccgg cgacgagcga acagcaggcc catatcccac aacacagcac tattcagaga
     6961 ggaacacacg tgcacgcaca acacacagcc cgcgcgatcg gcgcggcgct cttgacggcc
     7021 ggcgtcgtcg cggcgacact caccggctgc tctagcacca caaacaccag cggaagcaag
     7081 acagcaagtt caggctccag cacctacacg ttctgggacc catacccgca atacgacgcg
     7141 agttccgact ggggaaaact cgtcgcccaa tgcggcacca aggcgggagt cacggtcaag
     7201 agaacgggct acgacaccag cgacctgacc aataaggcgc tgctcgccgg ccagcaaggc
     7261 aactcgcccg acgtgctgct cgtcgacaac cccgtggtct ccaccctcgc atcggccggg
     7321 ctcctcaccg acacggcgga caacggcctc tcgaccggaa agatcgcgca gaacatcctc
     7381 ggggccggcg tcatcgacgg caagacctac ggggtgccga tcggcgcgaa cacgctggcc
     7441 ttgtactaca acccgaaaat cctcaaggcc gccaacgtcg atccggcatc gatcacgaac
     7501 tggtccaccc tcacatcggc gctcgccaag gtcacggcga gcggcaagaa gggcatcacc
     7561 tttgccggaa tcggaaccga agagggttcc ttccagttct tgccgtggtt ctggggtgcc
     7621 ggcgcgaacc tcaccaagct ggattcccca caggcgaacg ccgctctcag cctctggact
     7681 aactggatca agaagggcta cgccccgaac tcggtcatcc aagacacgca gacgacgagc
     7741 tggcaggagt tccagaccgg cgacttcgcg ttcggcgaga acggcacgtg gcagaa
//
LOCUS       DASTNL010000223         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1462298, whole genome shotgun sequence.
ACCESSION   DASTNL010000223 DASTNL010000000
VERSION     DASTNL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462298"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /gene="rpsA"
                     /locus_tag="VFQ96_07170"
     CDS             <1..1448
                     /gene="rpsA"
                     /locus_tag="VFQ96_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU0257603.1"
                     /translation="QAEQVAINDIGSAEDFLAAVEKTLKFFNDGDLISGTVVKIDRDE
                     VLLDVGYKTEGVIPSRELSIKHDVDPSEVVEVGDEVEALVLQKEDKEGRLILSKKRAQ
                     YERAWGDVERIKEDDGVVTGTVIEVVKGGLIVDIGLRGFLPASLIELRRVRDLTPYLG
                     QELEAKILELDKNRNNVVLSRRALLEETQSATRASFLANLHKGQIRKGTVSSIVNFGA
                     FVDLGGVDGLVHVSELSWKHIEHASEVVEVGQEITVEVLDVDLDRERVSLSLKATQED
                     PWQVFARTHAIGQIAPGKVTKLVPFGAFVRVADGIEGLVHISELSNKHVELAEQVVAV
                     GEDLFVKIIDIDLDRRRISLSLKQANDGVDPDGTEFDPALYGMPTEYDENGEYKYPDG
                     FDAEAKEWKEGFEAEREKWEQDYAAAQARWEAHKKQVAKALEEDAQGAHSSSSSSTSS
                     SSFTGQPASGSTGTLADDASLAALREKLSSN"
     gene            1530..2201
                     /gene="coaE"
                     /locus_tag="VFQ96_07175"
     CDS             1530..2201
                     /gene="coaE"
                     /locus_tag="VFQ96_07175"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU0257604.1"
                     /translation="MPVIALTGGIASGKSTVAGHLARLGAVVIDADRVAREVVEPGTP
                     ALRLLRAAFGDDIVDEAGRLDRAALGRLVFADDEARRTLNSIVHPAVRARVEEKIRKA
                     LAADPSAVVVYDVPLLAEGGPAAAAGYDAVVVVDAARPRRIDRLVRLRGMSRDDALRR
                     VHAQADDQARLALADIVIDANGTLEETLTRADEAWRRLRALPAGRSGSRPASGSQNDE
                     RRRDA"
     gene            complement(2163..3083)
                     /locus_tag="VFQ96_07180"
     CDS             complement(2163..3083)
                     /locus_tag="VFQ96_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0257605.1"
                     /translation="MLATSILLGLVAVALAWPVPILLARSRWPLRAPGSALFAWQTVA
                     FAGGISMIGALAIFGLAPFGQTALKAGAGAAAWLGRTDGTTRVSVASVVALSVALLLA
                     LHLLLTLAVTWTHTGRLRRRHRALVALLGKTTPGDPRTRVLDVAAPVAYCLPGGASMT
                     VLSTGLVESLGHAELEAVIAHERGHALQRHHLVLVTFRAWHRALPWLPTARLAEEAVT
                     RLVELLADDYARHRVEDGVLRRAILQVTPTGSLGLDEAGAPVDGSLTLLRAQRLSSGS
                     TGLRLPYRLLVASASAALLCIPPALIVLRA"
     gene            complement(3093..3449)
                     /locus_tag="VFQ96_07185"
     CDS             complement(3093..3449)
                     /locus_tag="VFQ96_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0257606.1"
                     /translation="MATLGDLERAVMDTLWEAAEPLTSSEVQEAIGRVRDGSLAATTV
                     LTVLSRLEKKGFLARDRSTRPHLFRPTHTSTEYTAELMNQVLGSAKDRKAVLTRFVGE
                     VGPGDTEMLRRILESQ"
     gene            3523..>4481
                     /locus_tag="VFQ96_07190"
     CDS             3523..>4481
                     /locus_tag="VFQ96_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU0257607.1"
                     /translation="MELRRLARPFEVVSDYRPSGDQPQAIRELTARINAGETDVVLLG
                     ATGTGKSATTAWLIEQVQRPTLVLSHNKTLAAQLANEFRGLLPHNAVEYFVSYYDYYQ
                     PEAYVPQTDTYIEKDSSINDEVERLRHSTTNSLLTRRDVVVVSTVSCIYGLGPVEEYV
                     DAMVRLEVGQRVRRDDLIHGFIQMQYQRNDIDFSRGKFRVRGDTLEIIPMYEENAIRI
                     EMFGDEIEALYTLHPLTGQVVEKLDVVPVFPGSHYVASTDVMQRAIGTIRIELEERLA
                     ELEREGKLLEAQRLRMRTTYDLEMMRQVGSCSGIENYSRHIDGR"
BASE COUNT          776 a         1547 c         1510 g          648 t
ORIGIN      
        1 agcaggccga gcaggtcgcg atcaacgaca tcggctccgc cgaagacttt ctggccgcgg
       61 tcgaaaagac acttaaattc ttcaacgacg gggatctcat ctctggcacc gtcgtgaaga
      121 tcgatcgcga cgaggtcctc ctcgacgtcg ggtacaagac cgagggcgtc atcccctcgc
      181 gcgagctttc catcaaacac gacgtcgacc ccagcgaggt cgtcgaggtc ggcgatgagg
      241 tcgaggccct cgtcctccag aaagaggaca aagaaggccg cctcatactg tcgaagaagc
      301 gcgctcaata cgagcgggct tggggcgacg tggaacgcat caaagaagat gacggcgtcg
      361 tcaccggcac cgtcatcgag gtcgtcaagg gcggcctcat cgtcgacatc gggctgcgcg
      421 gcttcctgcc cgcctccctc atcgagctgc gccgggtccg cgacctcacg ccctacctcg
      481 gccaggagct cgaggcgaag atcctggagc tcgacaagaa ccgcaacaac gtggtcctct
      541 cccgccgggc gctcctcgag gagacccagt cggcgacccg tgcctcgttc ctcgcgaacc
      601 tgcacaaggg gcagatccgc aaggggacgg tatcgtccat cgtcaacttc ggcgcgttcg
      661 tcgacctggg cggcgtagac ggcctggtgc acgtctccga gctgagctgg aagcacatcg
      721 agcacgccag cgaggtcgtc gaggtcgggc aagagatcac cgtcgaggtc ctcgacgtcg
      781 atctcgaccg ggagagagtc tccttgtctc tcaaggcgac acaggaagac ccgtggcagg
      841 tcttcgcccg cacgcacgcg atcggccaga tcgcccccgg taaggtgacg aagctcgtgc
      901 cgttcggtgc gttcgtgcgc gtcgccgacg gcatcgaagg cctcgtacac atctccgagc
      961 tgtcgaacaa gcatgtcgag ctcgccgagc aggtcgtcgc cgtcggcgaa gacctcttcg
     1021 tgaagatcat cgacatcgac ctcgatcgcc gccgcatctc gctgagcctc aagcaggcca
     1081 acgacggcgt cgacccggac ggcacggagt tcgacccggc cctgtacggc atgccgaccg
     1141 agtacgacga gaacggggaa tacaagtacc ccgacgggtt cgacgccgag gccaaggagt
     1201 ggaaggaagg cttcgaggcc gagcgcgaga agtgggagca agactacgcg gccgcgcagg
     1261 cccgttggga ggcccacaag aagcaggtcg cgaaggccct cgaagaagac gcgcagggcg
     1321 cgcactcctc gtcgtcttcg tcgacgagct cctcctcgtt caccggtcag cccgcctccg
     1381 gcagcaccgg cacgctcgcc gacgacgcgt ctctcgcggc gttgcgtgag aagctctcca
     1441 gcaactgacg cccttgcgtc gataagcggc accgtccctt tgggcggtgc cgctttgtcg
     1501 tgcggggcct tcgaccgtag gctgagcccg tgcccgtcat cgcgctgacc ggggggatag
     1561 cctccgggaa atcgaccgtc gccgggcacc tggcaagact cggcgccgtg gtcatcgacg
     1621 cggaccgggt cgcgcgcgag gtcgtcgagc cgggtacccc ggcgctgcgg ctccttcgtg
     1681 ccgcgttcgg cgacgacatc gtcgacgagg ccggccggct cgaccgggcg gcgcttggcc
     1741 gcctcgtctt cgccgacgac gaggcgcgtc gcacgctgaa cagcatcgtc caccccgcgg
     1801 tgcgtgcgcg cgtcgaagaa aagattcgca aggccctcgc cgccgacccg tcggccgtcg
     1861 tcgtctacga cgtgcctctt ctcgccgagg gcgggccggc cgccgcggcc ggctacgacg
     1921 cggtcgtcgt ggtcgacgcc gcgcgcccgc gccgcatcga ccggctcgtg aggctccgcg
     1981 gcatgtcgcg cgacgatgcg ctgcgccgag tgcacgccca ggccgacgac caggcgaggc
     2041 tggcgctcgc cgacatcgtg atcgacgcga acggcacgct cgaggagacg ctcactcggg
     2101 cagacgaggc ctggcgacgc cttcgcgcgc tgcctgccgg ccggtccggc agcaggccgg
     2161 cgtcaggctc gcagaacgat gagcgccggc gggatgcata agagcgcggc ggaggcggag
     2221 gcgacgagca ggcggtacgg cagcctgagg cccgtcgacc ccgacgagag gcgctgggcg
     2281 cgcaacaagg tcagcgagcc gtcgaccggc gcgccggcct cgtccaggcc gagcgacccc
     2341 gtgggcgtca cctgcaagat cgcgcgccgc agcacgccgt cttccacgcg gtgccgcgcg
     2401 tagtcgtcgg ccagcagctc gacgaggcgc gtcaccgcct cctccgccag ccgcgccgtc
     2461 ggcagccagg gcagcgcccg gtgccacgcc cggaaggtga cgagcacgag gtgatgtcgc
     2521 tgcagggcgt ggccccgttc gtgggcgatc acggcctcca gctcggcgtg gccgagactc
     2581 tcgacgagcc ccgtcgacaa gaccgtcatc gaagctccgc ccggcaggca gtaggcgacg
     2641 ggtgccgcca cgtcgagcac cctcgtgcga gggtcgcccg gcgtggtctt gcccagcagg
     2701 gcgacgagcg cacgatgccg gcgccgcagc cgcccggtgt gggtccacgt gacggcgagg
     2761 gtcaagagca ggtgcagggc gaggaggagc gcgacgctga gcgcgacgac ggaggcgacg
     2821 ctgacccgcg tggtgccgtc cgtacgcccg agccaggccg ccgccccggc gccggccttg
     2881 agggccgtct ggccgaaagg tgccaggccg aagatcgcca gcgccccgat catgctgatg
     2941 ccgcccgcga acgccacagt ctgccaggcg aagagtgcgc taccgggtgc cctgagcggc
     3001 catcgggaac gcgccagcag aatcggcacg ggccatgcca aggccaccgc cacgaggccg
     3061 agcagaatcg acgtcgcaag caccggccgc actcactgtg attcgaggat gcgtcgcaac
     3121 atctcggtgt cgccgggacc cacctctccg acgaagcgtg tgagcacggc cttgcggtct
     3181 ttcgccgagc caagcacttg gttcatgagc tctgccgtgt attcggtgct cgtgtgggtg
     3241 gggcggaaca ggtgcggcct tgtgcttcga tcgcgcgcca gaaagccctt tttctccagc
     3301 cgggagagca cggtgagcac ggtcgtcgcg gcgagagagc cgtcgcgcac ccggccgatc
     3361 gcctcctgca cctcggagga ggtcagcggt tcggccgcct cccacagggt gtccatgacg
     3421 gcacgttcga gatcccccaa agttgccacg cagtcatgtt agtggaggct cctgagggcg
     3481 cccttcaggc ggccttcgcc gtgccgtact taaactgcat tcatggaact tcgtcgtctc
     3541 gcgcgcccgt tcgaggtcgt cagcgactac cggccgagcg gcgaccagcc gcaggcgatc
     3601 cgggagttga cggccaggat caacgcgggc gagaccgacg tcgtgctcct gggcgcgacc
     3661 ggcaccggca agtcggcgac gaccgcctgg ctcatcgaac aggtgcagcg ccccaccctc
     3721 gtgctttcac acaacaagac cctggcggcc cagctggcca acgagttccg cggccttctg
     3781 ccgcacaacg ccgtcgaata cttcgtctcg tactacgact attaccagcc ggaggcatac
     3841 gttccgcaga ccgacaccta catcgagaag gactcgtcca tcaacgacga agtcgagcgg
     3901 ctgcgacact ccacgacgaa ctctctcttg acccgccgcg acgtcgtcgt cgtctcgacc
     3961 gtctcgtgca tctacggcct ggggcccgtc gaggagtacg tcgacgcgat ggtccggctc
     4021 gaggtggggc agcgggtccg ccgcgacgac ctcatccacg gcttcattca gatgcagtat
     4081 cagcgcaacg acatcgattt ctcgcgcggc aagttccggg tgcgaggcga cacgctcgag
     4141 atcatcccga tgtacgagga gaatgcgatc cgcatcgaga tgttcggcga cgagatcgag
     4201 gcgctgtaca cgctccaccc gctcaccggg caggtcgtcg agaagctcga cgtcgtgccg
     4261 gtgttccccg ggtcgcacta cgtcgcgagc accgacgtga tgcagcgggc gatcggcacg
     4321 atccgcatcg agctcgagga gcggctcgcg gagctcgaac gcgaaggcaa gctgctcgag
     4381 gcgcagcggc tgcggatgcg caccacctac gacctggaga tgatgcgcca ggtcggctcc
     4441 tgctccggca tcgagaacta cagccggcac atcgacggcc g
//
LOCUS       DASTNL010000224         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_351838, whole genome shotgun sequence.
ACCESSION   DASTNL010000224 DASTNL010000000
VERSION     DASTNL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351838"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..727
                     /locus_tag="VFQ96_07195"
     CDS             116..727
                     /locus_tag="VFQ96_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0257608.1"
                     /translation="MAKREKSEAESRGRILDAAERLFASRGFDGTATSSIARTAGVPK
                     GLLFYYFPSKLDILSTLLGERLAHAWLDPQLLAVPGNPVRALLNVSERLLRAQDESGV
                     LRVIVWHEEHVAPEVRAALARYRHALHDTIEQVLQASLAAPVAAGALRAAASTWAAIV
                     TARPLEGLKDAGGRHTAALHAKESLRSMAELICSGLRALPALP"
     gene            909..1778
                     /locus_tag="VFQ96_07200"
     CDS             909..1778
                     /locus_tag="VFQ96_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0257609.1"
                     /translation="MATAQVTAERAGQITLGGELSVNRLGYGAMQLTGKGVWGPPADP
                     DEAMRVLRRAVELGVTFIDTADSYGPFVAEELIRKALHPYPDDLVIATKAGQVRPGPD
                     KWVPLGRPEFLRQEVEMSLMRLGVERIDLLQLHRIDKTVPLEDQIGELAKMRDEGKIR
                     YLGISEVSVAQLQAALAVAPIVAVQNRYNLAVRDAEAVLDYAEANGLAFIPWFPLETG
                     KLAAEAGPTAAIAERLGATPSQLALAWLLRRSKSILPIPGTSKVAHLEQNIAAAGLEL
                     TDDDVAALTAAVA"
     gene            1869..2984
                     /locus_tag="VFQ96_07205"
     CDS             1869..2984
                     /locus_tag="VFQ96_07205"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEU0257610.1"
                     /translation="MGSVNEGSVLAEMLHHRAVGLAPGEPVALPLVSASMYFLPGVPD
                     APFQYGRSGGPTWSALEEALGILEQAEVIVFPSGMAAIQAVLMTRLSPGDRVLVPSDG
                     YYNTRTLLKDFLAPLGIRYDLCATHDYADRDFGGYALVYVETPSNPALDVCDIADVAA
                     RAHAAGALVVVDNTTMTPLGQRPLDLGADLVVASDTKAPGGHSDALLGHVAARDGSLL
                     DPVRRWRTVSGAIPGQLETWLVHRGLESLEVRFDRMCASAGVLAERLAGHAAVTGVAY
                     PGLEAHPDRAVVRRQMLRCGSLVGVTLESARAAEAFIDGARFVVPTTSFGGTHTTAER
                     RARWGDDVAEGFVRLSVGCEPVEALWADLVHALDAVC"
     gene            complement(3337..3657)
                     /locus_tag="VFQ96_07210"
     CDS             complement(3337..3657)
                     /locus_tag="VFQ96_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257611.1"
                     /translation="MSEMVSDDIVNLSRDGGALLVRDAFTVGFTQPCRELSRQWHGQD
                     DQQLHTRVGADEGRKGQRNRADLADRKRPPRIADEQARSDDVRVGRCRHSAKLPVQLS
                     ATKP"
     gene            complement(3711..3875)
                     /locus_tag="VFQ96_07215"
     CDS             complement(3711..3875)
                     /locus_tag="VFQ96_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257612.1"
                     /translation="MTGPDKVSHKRVGGFSLGMGQRLGIAAALLGDPETLILDKPVNG
                     LAPDGVLWAR"
     gene            complement(4039..4218)
                     /locus_tag="VFQ96_07220"
     CDS             complement(4039..4218)
                     /locus_tag="VFQ96_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257613.1"
                     /translation="MIEIEHVSKRYGPKLAVDDISFTVRPGAVTGFLGPNGAGKSTAM
                     RMIWGWIAPRTAARW"
BASE COUNT          660 a         1559 c         1559 g          739 t
ORIGIN      
        1 gtcggcacgc ccgcccagca cactcggcaa agtcacgggc tgcgtgatgg tcgcggggat
       61 aggcgcgccg gtataacgga taacgaacga gatcgtcgtc ggaaagcagg gcacggtggc
      121 caaaagagag aagtcggagg ccgagtctcg cgggcgcatc ctcgacgccg ccgaacgatt
      181 gttcgcctcg cgcgggttcg atggcaccgc gacctcgtcg atcgcccgca cggcaggagt
      241 gccgaagggc ctgttgttct attacttccc ctcgaagctc gacatcctct cgacgttgct
      301 gggggagcgg ctggcacacg cctggctcga cccgcaactc ctcgcggtgc cgggcaatcc
      361 ggttcgggct cttctgaacg tgagcgagcg gcttttgcga gcccaagacg agtcgggcgt
      421 gctgcgtgtc atcgtctggc acgaagagca tgtcgccccg gaggtgcgag cggcgttggc
      481 ccgctatcgt cacgccttgc acgacacgat cgagcaggtc ttgcaggcca gcctcgcggc
      541 acccgtcgcg gcgggcgcgt tgcgcgcggc ggcctcgacg tgggcggcga tcgtcacggc
      601 gcgccccctc gagggactca aagacgcggg cggccgtcac actgcggcct tgcacgccaa
      661 agagagcctg cgctcgatgg cagagctcat ctgctccggc ttgcgggcgc tgcccgcctt
      721 gccctgagcc tgcgctttgg gggtagcggg ccgcctcgcc tgccgtggcc tcgcggtttc
      781 cgtaccggca taggcggccg cgcgtacatt ggacgagggc cggcggcggg tacgtgcgcc
      841 ggagcccgtg atcggggcga ccgaggccgc ggccgggcgc cggccggcga cagggatggg
      901 atggtgttat ggcgacagca caggtgacgg cggaacgggc agggcagatc acgctcggcg
      961 gcgaactgag cgtgaaccgg ctcgggtacg gcgccatgca gctgacgggc aagggagtat
     1021 gggggccgcc tgccgacccg gatgaggcga tgcgggtgtt gcgccgcgcc gtcgagttgg
     1081 gtgtgacgtt catcgacacg gccgactcgt acggcccgtt cgtggcggaa gagctcatcc
     1141 gcaaggccct ccacccgtac ccggacgatc tcgtgatcgc gaccaaagcc gggcaggtgc
     1201 gcccggggcc cgacaagtgg gttccgctcg gccggccgga gttcctgcgg caagaggtcg
     1261 aaatgtcgct gatgcgcctc ggtgtcgagc gcatcgacct tctgcagttg catcgcatcg
     1321 acaagaccgt gccgctggaa gaccagatcg gcgaactggc gaagatgcgc gacgagggca
     1381 agatccgcta cctcggcatc tccgaggtga gcgtcgcgca gctgcaggcg gccctggcgg
     1441 tcgcgccgat cgtcgccgtg cagaaccggt acaacctcgc ggtgcgcgac gccgaggcgg
     1501 tgctcgacta tgcagaggcg aacgggctgg cgttcatccc gtggttcccg ttggagacgg
     1561 gcaagttggc ggccgaagcc ggcccgacgg cggcgatcgc cgaacggctc ggtgcgaccc
     1621 cgtcgcagct ggcgctcgcc tggctgctgc gtcgctcgaa gagcatcctg cccatccccg
     1681 gcacctcgaa ggttgcccac ctggagcaga acatcgccgc cgccggcctc gagctgacgg
     1741 acgacgacgt ggcggcgctc accgctgccg tcgcctgacc gcctcgggcg tcgccgaacg
     1801 cctgaggcgg gcgacgcgct acgttggctc ctggaagaga cggttgtcgg ggtgtcgaag
     1861 gggagcgcgt ggggtccgtg aacgaaggtt ccgttctggc cgagatgctg catcatcgag
     1921 cggtgggcct tgcaccgggg gagccggtcg cccttccgct cgtgtcggcc agcatgtact
     1981 ttctgccggg cgtgccggat gcgccgttcc agtacggccg atcgggcggg cccacctggt
     2041 cggccctcga agaggccctg ggcatcttgg agcaggcaga ggttatcgtc ttcccgtcgg
     2101 gcatggcggc gatccaggcg gtgctcatga cccgtctctc gcccggcgac cgggtgctgg
     2161 tcccctccga cggctactac aacacccgca ccctgctgaa agactttctt gccccgctcg
     2221 gcatccggta tgacctctgc gccacccacg actacgcgga ccgagacttt ggcggctatg
     2281 ccctcgtcta tgtcgagacc ccgtcgaacc cggcgctcga cgtctgcgac atcgccgacg
     2341 tcgcggcccg ggcccacgcc gcaggcgccc tcgtcgtcgt cgacaacacg acgatgaccc
     2401 cgctgggtca gcggcccctc gacctcggcg ccgacctcgt cgtcgcctcc gacacgaagg
     2461 cgcccggcgg ccattctgac gcgctcttgg ggcacgtcgc cgcgcgcgac ggttctctcc
     2521 tcgaccccgt gcgccggtgg cggaccgtgt cgggcgccat cccggggcaa ctcgagacct
     2581 ggctcgtgca tcgggggttg gagagcctgg aggttcggtt cgaccgcatg tgcgcgagcg
     2641 ccggggtgct tgcggagcgc ctcgccgggc acgccgcggt gacgggcgtc gcctacccag
     2701 gcttggaggc gcatcccgac cgcgcggtcg tgcgccgcca gatgctgcgc tgcggaagcc
     2761 tcgtgggggt caccctcgaa agcgcccggg ctgctgaggc gttcatcgac ggcgctcgtt
     2821 tcgtggttcc gacgacgagc ttcggcggca cgcacacgac ggccgagcgc cgggctcgct
     2881 ggggtgacga cgtggccgag ggtttcgtgc ggctctccgt cggctgcgaa cccgtcgagg
     2941 cgctgtgggc agacctggtg cacgccctcg atgcggtgtg ctgacggtcg gatccgttat
     3001 cagatgcgcc tgccggtcgg gcggccagca ccggttcgcc gcgttcttgc accgcgcggt
     3061 ttaggtgacg acgagctaaa gcacccgccg ccgcccgctg aaacgtccgg ggcgtgggca
     3121 gggcgctgcc cgtcacgaac gccgcggcct tacgaaaatc cgccgaatgg atgacccctc
     3181 gcttccgtac actgacaccg tagcggcgcg acgtgaacgg tgggcgctcg atgtcgccga
     3241 ggcgatgttg cgcggcttca acttcgtcgc tgttgtcgtg gtcgcctacg ccctgcggca
     3301 tcgcacgggg atctgggatc cgcgctcggt cgtcgattac ggcttggtcg cgctcagttg
     3361 caccgggagc ttcgcactgt ggcggcacct acccacgcgc acgtcgtcgg agcgtgcttg
     3421 ctcgtcggca atccgtggtg gtctttttcg gtcggcgaga tccgcgcgat tccgctggcc
     3481 tttgcggcct tcgtcagcgc ctactcgggt gtgaagctgc tggtcgtcct ggccgtgcca
     3541 ctgtcggctg agttcccggc atggctgcgt gaatccgacc gtgaacgcat cgcgaacgag
     3601 gagcgcaccg ccatcgcgcg agaggttcac gatgtcgtca ctcaccatct cgctcatcag
     3661 atgcgaggac aggaacacgg tgcggccctg ggaggccagt accgagtcaa ctaccgcgcc
     3721 cacaacaccc cgtccggggc caggccgttc accggcttat ccaggatcaa cgtctccggg
     3781 tcgcccaaca aggcggccgc gatgcccagg cgctgcccca tcccaagcga gaagccaccc
     3841 acccgtttat gggacacctt gtccggcccc gtcatctcga tgacctcgcc cacccgggtc
     3901 ttcggcagcg aatgcgtggc cgccgacgcg agcagatggt tgtaggcgct acggccggtg
     3961 tggtgtgcac ggccttcgcc tccagaggcg cagccacctc gtgcagcggg tcacggtgct
     4021 gtccgtacgg cttcccgttc accatcgtgc ggccgtgcgt ggggcgatcc agccccagat
     4081 catccgcatc gccgtggact tccccgcccc gttcgggccc aggaaacccg tcaccgcccc
     4141 cggccgcacc gtgaacgata tgtcatccac cgccagcttc ggcccatacc gtttgctcac
     4201 atgctcgatc tcaatcacaa caccaacgct agaaaccacg cccgccacac acatccaccc
     4261 acggtccccg ctccccacga cgagcacccc accacggtct aaggcaccct cgccgggtac
     4321 cctccccgac gagcgtgtgc ttttggccgt acccgggccc ggtgtttacg atgcagagat
     4381 gaccctgccg cgtacacccc cgggtggcgg gcgtgtcgcg gtggtgtgga acccggccaa
     4441 gctcgaccct gtccggctga gggaggccgt gagggtggcg gaatcggaaa ccggccgggc
     4501 gcggtcgctg tggtttc
//
LOCUS       DASTNL010000225         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1092801, whole genome shotgun sequence.
ACCESSION   DASTNL010000225 DASTNL010000000
VERSION     DASTNL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092801"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFQ96_07225"
     CDS             complement(<1..302)
                     /locus_tag="VFQ96_07225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0257614.1"
                     /translation="MPPTPVETSVVICAYTQARWHDMNAAIASALRQPETDEVIVVID
                     HEDDLLRRTRQRWPHLSVVANTFQRGLSGARNSGVLAASGGVVAFLDDDATAEEGWL"
     gene            complement(302..1237)
                     /locus_tag="VFQ96_07230"
     CDS             complement(302..1237)
                     /locus_tag="VFQ96_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0257615.1"
                     /translation="MSDSLHVSRVLQQQRDSEQRNSHERKHNETEIRRLPGTGTPTVS
                     IVIPARNEAKNLEQVLPTLPAVHEVILVDGASSDGTVETAQRVMPKIRVIHQTRRGKG
                     NALACGFSAATGDIIVMFDADGSADPAEIASYVAALTDGADFAKGTRFSPGGGSEDIT
                     RLRQAGNLALNAITNLLFRARYSDLCYGYNAFWRRVLPHLDLPDVRLPERPDGKMRWG
                     DGFEIETILSCRITAAKMIVAEVPSVERSRIYGASNLNAVTDGLRVLKSILTEHRRAR
                     SANAGARFTTRRRPAVLRRVSVGRIAEIAERSEAA"
     gene            2118..>2704
                     /locus_tag="VFQ96_07235"
     CDS             2118..>2704
                     /locus_tag="VFQ96_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0257616.1"
                     /translation="MERTKAGRASDGYTAVRRRWGRTFFVALVAAATLLVTVQLATPP
                     TASAAVTCAKGSALDVVAHPDDDLLFQSATIRADIDAGKCVRTVYVTSGDDGLPSWYW
                     QSRESGVQAAYAQMAGVANRWSTSDASVPGHPIVLKTLRSAPEISLVFLRLPDGQMDG
                     SGSADNNYESLQKLYQHVIGTIHAIDGSTSYTEASL"
BASE COUNT          415 a          802 c          939 g          548 t
ORIGIN      
        1 agccaccctt cctcagcggt ggcgtcgtcg tcaagaaagg cgacgacgcc accggaggcg
       61 gcgagcacgc ccgagtttcg tgctcccgag aggccgcgct gaaacgtgtt ggctaccacg
      121 ctcaggtgcg gccagcgttg ccgggtgcgg cgaagcaggt cgtcttcgtg gtcgatgacg
      181 acgatgacct cgtcggtctc cggctgtctg agggccgagg cgatcgcggc gttcatgtcg
      241 tgccagcgcg cttgcgtgta ggcgcagatg acgacgctcg tctcgacggg tgtggggggc
      301 atcatgccgc ctcgctgcgc tcggcgatct ccgcgattcg gccgacggac acgcggcgca
      361 gcacggccgg ccggcgccgg gtggtgaagc gtgctccggc attcgcgctg cgggcccggc
      421 ggtgctcggt caggatggat ttcaagacac gcagcccatc ggtcaccgcg ttcaggttgc
      481 tggccccgta gatgcggctg cgttccacgc tcggcacctc ggcgacgatc atcttggcgg
      541 ccgtgatgcg gcagctgagg atcgtctcga tctcgaaccc gtcgccccaa cgcatcttgc
      601 cgtcgggcct ttcgggcagc ctcacgtcgg gcaggtcgag gtgtggcaga acgcggcgcc
      661 agaaggcgtt gtagccgtag cagaggtcgc tgtagcgggc gcggaacagc agattcgtga
      721 tcgcgttcag tgcgaggttg ccggcctggc gcagccgtgt gatgtcttcg ctgccgccgc
      781 cgggcgagaa ccgcgtgccc ttggcgaagt cggcgccgtc tgtcagcgcg gccacgtacg
      841 aggcgatctc cgccgggtcg gcggaaccgt cggcgtcgaa catcacgatg atgtcgcccg
      901 tggcggccga aaagccgcag gccagggcgt tgcctttgcc gcgccgggtc tggtggatca
      961 cccgaatctt cggcatcacc cgttgtgctg tctcgaccgt gccgtcactg gaggcgccgt
     1021 cgaccaggat cacctcgtgc acggccggca gtgtgggcag tacctgttcc aggttcttcg
     1081 cctcgttgcg ggccggaatg acgatggaga cggttggggt gccggtgccg ggcagccgcc
     1141 gaatctcggt ttcgttgtgc tttcgttcgt gagagttccg ttgttcggaa tctcgttgtt
     1201 gttgcaatac tcgggagacg tgtaatgagt cggacacgcg aaggctcctc ataactcggg
     1261 tacatcggaa gaacggtcgt ctgttcgccg cggagcgcga cgaagcgatg tataggggag
     1321 tcgacctaaa aagttgggct ccacgagcca gcccgccgag gctgtaggtc ttcgtgcctc
     1381 atagtgttct cggtgtggca aagcccttga gaggtgacag acgtagcggt gtaggcctcg
     1441 gccgaagcgg tatagcctta ccgattcggg tctgtcggcg ccgtcatgct ttctggtgtc
     1501 ggagcggttg gttgcctagc caatcgccgg caccagactt atcagtgtgg aattgcggct
     1561 tgcggcgaag agttggtgcg ctgcacctcg ttttcgccgt gcacacgctg gtatgtgtgc
     1621 ggggtgaaag gtgttgtgcg ttggcatact gcctcccctc gcggtgagtc ccgacatgct
     1681 gactgcggga ttcgggttgt gttcttttgc gttcctacga cgtctcccag tatgcgagac
     1741 ggacccccgc ttggtcgtaa ccgtgtgaca agacctggtg aggattaggt caatgttccc
     1801 ggaactttta tcgaatgcac gtggcggatc gagttcttgt gaagagcctg gtgcgtatgt
     1861 gaagagggct tggttgttga cagttttttg acgcgcgctc gacatcaggt gaacgcgggg
     1921 tgaacgaagg cagtggaaga taagagacac gtcgtcatgg ggcgggtggt gctctttatg
     1981 gtccttctgg ctaaggggct gggggcagca ccgcccctat cgctgtcggc gttcgtgtgt
     2041 gtctatgcag cgtgatcgct cgtgacgaga agcgaggtgt ggccatgacc ggtgtcccga
     2101 ggaaggccgg taggcgggtg gagcgaacga aggccggtcg ggcatccgac ggctacactg
     2161 cggtgcgtcg ccgatggggg cgcacgttct tcgtggcgct cgtggccgcg gcgacgctgc
     2221 tcgtcaccgt tcagctggcc acgccgccca cggcctccgc cgcagtcacc tgcgccaagg
     2281 gttccgcgct tgacgtcgtc gcgcaccccg acgacgacct gctctttcag tcggcgacga
     2341 tccgcgccga catcgatgct ggaaagtgcg tgcgcacggt ttacgtgacc tccggcgacg
     2401 acggcctgcc ctcgtggtac tggcagtcac gcgagtcggg cgtgcaggct gcttacgcgc
     2461 agatggccgg cgtcgcgaac cgttggtcca cctccgacgc gagtgttccg gggcacccga
     2521 tcgttctgaa gactctccgg tctgcacccg agatctctct cgtcttcctg cgcctgcccg
     2581 acggccagat ggatggcagc ggcagcgccg acaacaatta cgagagcctg cagaagctct
     2641 accagcatgt gatcggcacg atccatgcga tcgacggttc cacctcgtac accgaggcgt
     2701 cgct
//
LOCUS       DASTNL010000226         6549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_538761, whole genome shotgun sequence.
ACCESSION   DASTNL010000226 DASTNL010000000
VERSION     DASTNL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538761"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFQ96_07240"
     CDS             <1..859
                     /locus_tag="VFQ96_07240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0257617.1"
                     /translation="AADAGVTVFDTADVYGDGRSESLIGRFLRAHPDLAVTVTTKMGR
                     RAEQQPENYTAENFRAWTDRSRRNLGTERLDLVQLHCPPTAVFASDRVFDALDALVEG
                     GAIARYGVSVETCDQALTAIARPGVASVQIILNAFRLKPLDAVLPAARAAGVAIIARV
                     PLASGLLSGRYTKETTFAENDHRTFNRHGEAFDRGETFSGVDFAEGVAAAREFSALAP
                     AGVTPAQAALAWVAAQDGVTTVIPGARSPEQARQNAAAGSLTLPQGFDEQVHDIYDRR
                     LRAAIHPQW"
     gene            925..1404
                     /locus_tag="VFQ96_07245"
     CDS             925..1404
                     /locus_tag="VFQ96_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0257618.1"
                     /translation="MAGSTVVLAPELEVSEWVGTPTPLSSLRGRVVMIEAFQILCPGC
                     IAYGLPQARKVQRMFPDVTVVGLNTVFEHHEVTGPDALRVFLAEYGYTFPVAFDRPGE
                     GPLPLTMQRYGLQGTPSTVLIDKSGTARFSHLGAIDDLALGMMLGRLVAEESPETVP"
     gene            complement(1434..1661)
                     /locus_tag="VFQ96_07250"
     CDS             complement(1434..1661)
                     /locus_tag="VFQ96_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEU0257619.1"
                     /translation="MDFLRVTPIGTRVVVRTRIPAGYTDALGYLRARDETTCFVETRR
                     KGTVRLLFADVVAAKEVPPPPARRQAMRPPA"
     gene            1780..2430
                     /locus_tag="VFQ96_07255"
     CDS             1780..2430
                     /locus_tag="VFQ96_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0257620.1"
                     /translation="MTHTDDAEGARVGRLVTRGVIHVDGRDVALENNVWLVGDANEVI
                     VIDPAHDPEATLAAVDGRLVTTILITHGHADHAGAALRFAELVDAPVFLHPADLELWS
                     ESHPDRQPDGVLVDEAVFTVRETPIRVLHTPGHTPGSVSFLAPTLGAVFTGDTLFEGG
                     PGATRWSYSSFPVIVDSIRTRLFTLPPQTVVHPGHGRSTTIGAERPQLAQWLARGW"
     gene            2682..3770
                     /locus_tag="VFQ96_07260"
     CDS             2682..3770
                     /locus_tag="VFQ96_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0257621.1"
                     /translation="MLEIRTLSKAYDAAGRHGEHIQALDSVDLEVADGEFLTVLGPSG
                     CGKTTLLRAIAGFEAPDAGTIRVAGRLVAGGSRPVPAHERGIGLVPQEGALFPHLSVA
                     QNVAFGLGNLKRKERQARVAETLELVGLAGFGPRRPHELSGGQQQRVALARAIAPGPR
                     IILFDEPFSALDEYLRESLRTEVRGLLADLHATVMLVTHDQEEALALGDRVAVMRDGR
                     VVQVGGPRETYYQPVDLALARFLGEAVVVQGDVARAEGQSPTVSCAFGTLPVAAWHGS
                     CGRCDVLIRPENIRVCRLAESLGGPRLHGTVGTVLDQTFYGHDGVVRVSVPEIAEHVS
                     VRVMGNRPFGVGEKVELAVDSAVCTYPG"
     gene            3831..5432
                     /locus_tag="VFQ96_07265"
     CDS             3831..5432
                     /locus_tag="VFQ96_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0257622.1"
                     /translation="MQHALRAHPVVTIVSMAVGLFSLLPFLYLFIAGFSVRRVHALFA
                     FPATLAELTRTVGLTAAISVCTVLLGVGAALLVVRSDLPFRRVLTVLFALPLAVPGFV
                     SAYAVYSAGLLVAPRSDLLTSFGGATIVISLTLYPYVFLACVVGARTIDPAQEEVARS
                     LGARPAAVLWRIVLPRLRPAIAGGVLIVALHVLSEYGAMVQLKQRTLTTEIMSQMLDY
                     GDYETARSLSMLLALLALVALVAGRGVSGRASEASIGAQATRPPGTVRLRGARLPVAA
                     VALAVPVAAVGPTVFMTVHGLTTPHRATALGWQQVAVAGANTIGYAVAAGLLASLVGF
                     PVSWWISRRPSTAATMTERSVWLAHAIPAAILALALVFLATRLAPGLYKTPAVLIAGY
                     VILFLPLAVANQRVGLESAFVRYDEAAAALGSPAWRRLGRVSLPLALPGVATGALLVG
                     LDASKELTTTLMLLPFNTQTLATGLWGTTNGQSLDFTAAAPYALVLIALGSIPVYLIT
                     RRTLRYLPATSVRPRQPAKSTAAAG"
     gene            5522..6109
                     /locus_tag="VFQ96_07270"
     CDS             5522..6109
                     /locus_tag="VFQ96_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257623.1"
                     /translation="MFSPAHVRAFAAFAALAFAVTLGACSTATPEAPHTTPTTSEASP
                     GAPASSQKPLFSTEKEALAAATKAYAAYLRVVDAIGRDGGAGLERLRDVTTGEQLVSS
                     TDSLTYYTEKNVRATGTTVFDSVSLQKWDARNSSASIYVCEDVSDIDMTDSNGKSIVS
                     ADREDRLPFIADFETASGKLSLSHKDLWLGDNFCQ"
BASE COUNT          987 a         2290 c         2214 g         1058 t
ORIGIN      
        1 ggcggcggat gcgggggtca cggtcttcga cacggctgac gtgtacggcg acgggcgcag
       61 cgagagcctg atcgggcgtt tcttgcgcgc gcaccccgac ctcgccgtca cggtgacgac
      121 gaagatgggc cggcgcgccg aacagcagcc cgaaaactat acggcggaga attttcgggc
      181 gtggacggat cgctcgcgcc ggaatctcgg caccgagcgg ctcgacctgg tgcagctgca
      241 ttgcccgccg acggcggtgt tcgccagcga ccgagtgttc gacgccctcg acgcgctggt
      301 ggagggcggc gcgatcgcac ggtacggcgt gagcgtcgag acgtgcgacc aggcgctgac
      361 ggccatcgcc cgccccggcg tcgcgagcgt gcagatcatt ctcaacgcgt tccgcctgaa
      421 gccgctcgac gccgtgctgc ctgcggcccg tgcagccggg gtcgcgatca tcgcccgggt
      481 gccgctggcc tccggcctgc tctccggccg atacacgaaa gagaccacgt tcgccgagaa
      541 cgaccaccgc acgttcaacc ggcacggcga ggcgttcgac cgcggtgaga ccttttcggg
      601 ggtcgacttc gcggaaggcg tggcggccgc gagggagttc tcggcgctcg cgcccgcggg
      661 cgtgacaccc gcgcaggccg cgctggcctg ggtcgccgcg caagacgggg tgacgaccgt
      721 cataccgggc gcgcgctcac ccgagcaagc ccggcagaac gcggcggcgg gcagcctgac
      781 gctgccgcaa ggcttcgacg agcaggtcca cgacatctat gaccgcaggc tgcgcgccgc
      841 catccacccg cagtggtgaa cccacatccg cacgccgtac gggcggtcgc ggcacgtcgc
      901 atcccacggt tctagtctgg aggcatggct gggtcgacgg tggtgctcgc tccggaactt
      961 gaggtctctg agtgggtcgg cacgccgacc ccgctctcgt cgctgcgtgg ccgcgtcgtc
     1021 atgatcgagg cgttccagat tctctgcccg ggctgcatcg cgtacgggct gccgcaggcg
     1081 cgcaaggtgc agcgcatgtt tcccgacgtg accgtggtgg gtttgaacac cgtcttcgaa
     1141 catcacgagg tgaccggccc cgacgcgtta cgggtgttcc tggccgagta cggctacacg
     1201 ttcccggtag ccttcgaccg gccgggggag ggcccactcc cgttgacgat gcaacgctac
     1261 ggcctgcagg gcaccccctc cacggtgctg atcgacaagt cgggcacggc tcggttctcc
     1321 catctcggcg cgatcgatga cctggccctg gggatgatgc tcggccggct cgtcgccgag
     1381 gaatcgcccg agaccgtgcc gtagcagccg ggacctgcag ggctgcccgg ctgttaggcg
     1441 ggcggcctca tcgcctggcg ccgcgcaggc ggcggaggca cctctttggc cgcgaccacg
     1501 tcggcgaaga gcagcctcac ggtgcctttc cgccgggtct cgacgaagca ggtcgtctcg
     1561 tcgcgcgccc gcaagtaccc gagggcatcc gtgtacccgg ccgggatgcg cgtgcgcacc
     1621 acgacccgcg tgccgatcgg ggtgacgcga aggaagtcca ccgggccatg tcgcacgctc
     1681 cccagtcaac cacgcacgtt ccacggcgcg aggcgagaag gcaggcacgc gcctgcccgc
     1741 cacggccggc cgacgatcgg gtcgtgagag gctgaggcca tgacacacac cgacgacgca
     1801 gaaggcgcta gggttggccg gctcgtgacg cgcggcgtca tccatgtgga cggccgcgac
     1861 gtcgcgttgg agaacaacgt ctggctcgtg ggtgacgcga acgaggtcat cgtcatcgac
     1921 cccgcgcacg atccggaggc gaccctggcc gccgtggacg ggcgcctcgt gacgaccatc
     1981 ctcataaccc acggccacgc cgaccacgcg ggggcggcct tgcgcttcgc cgaactggtc
     2041 gacgcgcccg tcttcttaca cccggccgac ctcgagctgt ggagcgagag ccaccctgac
     2101 cggcaacccg acggcgttct cgtcgacgag gccgtcttca ccgtgcggga aaccccgatc
     2161 cgcgtgctcc acacgccggg ccacaccccc gggtcggtga gcttcttggc gcccacgctg
     2221 ggcgccgtct tcacgggcga caccctcttc gagggcggcc cgggcgccac gcgctggagc
     2281 tacagcagtt tcccggtcat cgtcgattcg atccgtacga ggctcttcac tctcccgccg
     2341 cagacggtcg tgcatccggg gcacggccgc tcgacgacga tcggtgctga gcggccgcaa
     2401 ctcgcgcagt ggctcgcccg cggttggtga tgccgagtat ccgcggggcc ggccgcaaca
     2461 ccgcacgaaa gcttcgacaa gacgacggtg gggcacggag cctgccggcc ggccaggccc
     2521 tgtgatcgtg atgaggatgc ggacgcggga tgcacgacgt ggagcgacgg atgcggcgcg
     2581 cggcgagccc gggcgcggcc tcaggcagcg gcaggaattt aaacgaactc cctccgcaga
     2641 aaactcttaa ggtaaggctt accttccggt agggtggtgg tgtgctcgag attcggaccc
     2701 tgtcgaaggc gtatgacgcc gccggccgac acggcgagca tattcaggcg ttagacagcg
     2761 tcgatctcga agtggccgac ggcgagttct tgaccgtctt gggcccctcg ggctgcggca
     2821 agacgacgtt actacgggcg atcgcgggat tcgaggcgcc cgacgcggga acgatccggg
     2881 tggccggtcg gctggtcgcg ggcggctctc gccccgtgcc ggcccacgag cgcggcatcg
     2941 ggctggtgcc acaagagggc gcgctcttcc cgcacctctc cgttgcgcag aacgtcgctt
     3001 tcggcctggg caacctcaag cgtaaagaga ggcaggcccg agtcgccgag acgctggaac
     3061 tggtgggctt ggccgggttc ggccccaggc ggccacacga gctttccggc ggccagcagc
     3121 agcgtgtcgc cctggcgcgc gcgatcgcac ccggtccccg catcattctt ttcgatgagc
     3181 cgttctcggc cctcgatgaa tatctgcgcg agtctctgcg caccgaggtg cgggggcttt
     3241 tggccgacct gcacgccacg gtcatgctcg tcacccacga ccaagaagaa gccttggcgc
     3301 ttggcgaccg ggtggccgtc atgcgcgacg gtcgcgtcgt gcaggtcggc ggcccgcggg
     3361 agacctacta tcagccggtc gacctggcct tggctcgctt cctcggcgag gcagtggtcg
     3421 tgcagggcga cgtcgcacgg gcggaggggc agtccccgac cgtgtcgtgc gcgttcggca
     3481 cgttgccggt cgccgcatgg cacggctcgt gcggtcggtg cgacgtgctg atccggcccg
     3541 agaacatccg agtctgccga ttggcagaga gtcttggcgg gccccggctg cacggcaccg
     3601 tcggtacggt gctcgaccag accttctacg ggcacgacgg cgtcgtgcgg gtgagcgtgc
     3661 ccgagatagc ggagcacgtg tcggtgcgtg tgatgggcaa ccgcccgttc ggcgtcggcg
     3721 agaaggtgga gttggcggtc gactcggccg tgtgcaccta ccccggctga tccgccacaa
     3781 ccaggccgac aggcacccga accgacgaga gaccgaggcc gacgagcacc gtgcagcacg
     3841 cgttacgcgc ccatcccgtc gtcacgatcg tctccatggc cgtcgggctt ttctcgctgc
     3901 tgccgttcct ctacctcttc atcgccgggt tctccgtgcg gcgggtgcac gccctgttcg
     3961 cctttcccgc cacgctcgcg gagctgacgc gcacggtcgg cttgaccgcg gcgatcagcg
     4021 tctgcaccgt tctcctcggc gtcggggccg ccctcctcgt ggtgcgcagc gacctgccgt
     4081 tccggcgggt tctcacggtt ctcttcgcct tgccgctggc cgtgccgggc ttcgtcagcg
     4141 cgtacgcggt ctactccgcc ggcctcctcg tcgccccacg atccgacctc ctcacctcgt
     4201 tcggcggcgc gacgatcgtc atttccctca ccctctaccc gtacgtcttc ctggcctgtg
     4261 tggtcggggc ccgcaccatc gacccggcgc aagaagaggt ggcgcgcagc ctcggggccc
     4321 gaccggcagc cgtgctgtgg cgcatcgtgc tcccacgcct cagaccggcg atcgccggcg
     4381 gggtcttgat cgtggcacta cacgtgctct ccgagtacgg cgcgatggtg cagctgaaac
     4441 agcgcaccct gacgacggag atcatgtcgc agatgctcga ctacggagac tacgagacgg
     4501 cacgttccct ctcgatgctc ctggcccttc tcgccctcgt ggccctcgtg gccggccgcg
     4561 gcgtctccgg ccgtgcctca gaggcgagca tcggcgccca ggcgacgcgt ccccccggga
     4621 ccgttcggct gcggggcgcc cggctgccgg tcgccgccgt ggccctggcg gtgcccgtcg
     4681 cggcggtcgg cccgacggtg ttcatgaccg tgcacggcct caccaccccg caccgtgcca
     4741 ccgcgctcgg ttggcagcag gtcgcggttg ccggcgccaa cacgatcggt tatgcggtcg
     4801 cggccgggct gctggcgagc ctcgtcggtt tccccgtcag ctggtggatc agccgacgcc
     4861 cgtcgaccgc ggcgacgatg acggaacgtt ccgtctggct ggcgcatgcg atccccgccg
     4921 cgatcctcgc cttggcactc gtgttcctcg cgacgaggct cgcaccaggc ctctacaaga
     4981 cgccggcggt gctcatcgcg ggctacgtca tcctgttcct cccgctggcg gtcgcgaacc
     5041 agcgtgtcgg cctcgagtcc gcgttcgttc gctacgacga ggccgccgcc gcgctcggca
     5101 gcccggcgtg gcggcgcctt ggccgggtgt cgctgccgtt ggcgttgccg ggtgtcgcga
     5161 ccggcgcact gctcgtcggc ctcgacgcca gcaaagaact cacgacgacg ctcatgctgc
     5221 tgccgttcaa cacgcagacg ctcgcgacgg gcctgtgggg cacgacgaac ggtcagtcgc
     5281 tcgatttcac tgcggcggcg ccgtacgcgc tcgtgctcat cgccctcggc tcgattcccg
     5341 tatacctcat cactcggcgc acgctgcgct accttcccgc cacgagcgtc cgccctcggc
     5401 agcctgcgaa aagcaccgct gccgccgggt gacgacgcct gacgcgccgc cccgagcccg
     5461 gccgacaacg gcgtgtggat aatcctgagg cggcccgccg tgccgtggct agggtgtggg
     5521 aatgttttcc cccgcacacg tgcgcgcctt tgcggcgttc gcggcactcg cgttcgccgt
     5581 gacgctcggt gcctgcagca cggccacccc agaggcgcca cacacgacac cgaccacaag
     5641 cgaggcctcg ccgggagccc cggcgtcttc tcagaagccg cttttcagca cggagaaaga
     5701 agcgctggcc gcggcgacga aggcctatgc cgcctatctt cgggtggtag acgccatcgg
     5761 ccgtgacggt ggtgccgggc tcgaacgcct ccgcgatgtc accaccgggg aacagctcgt
     5821 aagttccaca gacagtctca cttactacac cgagaagaac gtccgcgcca ccggtacgac
     5881 agtcttcgat tcggtaagcc ttcaaaagtg ggacgcacga aactcgtcgg caagtatcta
     5941 tgtgtgcgaa gacgtatcag acatagacat gactgacagt aatggcaagt caattgtttc
     6001 cgcagatcga gaagaccggc tgccttttat cgcagatttc gagacagctt ccgggaaact
     6061 ctctctttcg cataaagatc tttggctggg agataacttt tgccaataac gcgtctaaaa
     6121 aaagtaccgc tcacattcct attggccacc cttgcggtgt tcatgctcgc gatgccggct
     6181 gacgccgata cggcccgatg cagtgaaaca cctcatcgtt cgggctcgtc gccacgacag
     6241 tgccacagtg ttacttcgag caatctcgga tccagtgtcg acctgaacgc ttcggcgacg
     6301 caaggtggca cgaagaccgg gcagaacggg tcgaccgcgg ggccgaaaag cacggcgccg
     6361 cgggggagta cacgggggtc cggggggagt accgccgccc ggccgaccgg cggccgaagc
     6421 cagacggtca agccgggcgc ggcccggaca cacccggctt ccccgtcaca gaagacggcc
     6481 acgccctccg tgtgcacgga gccgatgacg gaatggccgc tcgggtgtgc gatggatgcc
     6541 tacaggccc
//
LOCUS       DASTNL010000227         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1094487, whole genome shotgun sequence.
ACCESSION   DASTNL010000227 DASTNL010000000
VERSION     DASTNL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094487"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFQ96_07275"
     CDS             <1..285
                     /locus_tag="VFQ96_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEU0257624.1"
                     /translation="GAKQLFSAPPEARPDYILGDLRGLFEPYPETKTRRGSARCADAA
                     ARVDGAELIVDDRGTGLDRLRAAVHAVWSSGRAVYALNIPERALRLPGLP"
     gene            380..607
                     /locus_tag="VFQ96_07280"
     CDS             380..607
                     /locus_tag="VFQ96_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257625.1"
                     /translation="MSDGEGRRHGDDTVADVLDRDLFDARLRGIDALPLAARAAQFDR
                     IRLLLQDALDVADDDARPETVGERPGGTGRA"
     gene            604..1431
                     /locus_tag="VFQ96_07285"
     CDS             604..1431
                     /locus_tag="VFQ96_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU0257626.1"
                     /translation="MTGPPDGGEAGRKRRLDVAVASRGLARSRTHAARLIALGNVTVE
                     GETVTRPAFPVVPSARIVVRRDHYVGRGAVKLVAALDAFGLAVAGRQALDAGASTGGF
                     SQVLLERGASVVAVDVGHGQLAPELVDHPRLRAYDGVNVRSIDAEAYGRLTGGARRPD
                     LVVADLSFISLTQVIPALVATAAREADFVLLVKPQFEVGRRGVPAGVVRSARLREGAV
                     NGVVAALFRAGLGVAGIIPSPITGEKGNREYLVHATRRCRADPTEWEGRIRDMVGGR"
     gene            1428..2348
                     /locus_tag="VFQ96_07290"
     CDS             1428..2348
                     /locus_tag="VFQ96_07290"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU0257627.1"
                     /translation="MTRPGTRYILVVLHVGREDSVRAGAHVCRQLLGAGATPVLTSDG
                     LAALRRLDPGLGPLAELGDAVHAELLELVIVLGGDGTILRAAEMVRGYRAPLLGVNLG
                     HVGFLAESERDDLDEAVRRGLARDYEVEERMTLSVRVMVGDDVVYQSWALNEAAIEKA
                     SRERMLEVVIEVNRRPLSSFGCDGVVLSTPTGSTAYAFSGGGPIVWPTLEALLLVPLS
                     AHALFAKPLVVDAGAALAVEILERGGGAGVLWCDGRRTFDLPPGARVVVRRSPTAVCL
                     ARLHHGEFTDRLVRKFSLPVTGWRGPAGAD"
     gene            2385..>2737
                     /locus_tag="VFQ96_07295"
     CDS             2385..>2737
                     /locus_tag="VFQ96_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0257628.1"
                     /translation="MIEEIGIHGLGVIDDATLPLAAGFTALTGETGAGKTMVVSALGL
                     LLGQRADSGLVRPGSGVSVEGRWLVPGDGYVACRVREAGGIVDDAAADGAGAGEAGVV
                     VTRTLAASGRSKASIG"
BASE COUNT          334 a          984 c         1009 g          410 t
ORIGIN      
        1 ggggcgaaac agcttttctc ggccccgccc gaggcgcgac ccgactacat cctcggcgat
       61 ctgcgcggcc tcttcgagcc ctacccggag acgaagaccc gacgcggcag cgcccgctgc
      121 gccgatgccg cggcccgggt cgacggcgca gagctcatcg tcgacgaccg tggcaccggc
      181 ctcgaccggt tgcgcgccgc cgtccatgcc gtctggtcgt cgggccgcgc cgtctatgcg
      241 ctcaacattc ccgagcgggc cctgcgcctg cctggcctcc cgtgaggcgc ccgtcgcgcc
      301 cggggtgctt ggcggcggcg ccgccgggca gggcgcatgc cctcgggtgc tctgacgtac
      361 ggccctgtag cgtgggaata tgagcgacgg cgaggggcgg cggcacggcg acgacaccgt
      421 tgccgacgta ctcgaccgtg acctcttcga tgcccggctg cgaggaatcg acgcgttgcc
      481 gcttgcggcg cgtgccgctc agttcgaccg gatccgcctc ctccttcaag acgcgctcga
      541 cgtcgccgac gacgacgccc ggcccgagac ggtcggcgag aggccggggg gcacgggtcg
      601 ggcatgaccg gcccgcccga cggcggcgag gcgggccgca agcgccggct cgacgtcgcc
      661 gtggcaagcc gcggccttgc ccgctcccgc acgcacgccg cccggctcat cgccctgggg
      721 aacgtcaccg tcgagggcga gaccgtcacg cggccggcct tccccgttgt accgtccgcc
      781 cgcatcgtcg tgcgccggga ccactacgtc ggtcgcggcg ccgtgaagct ggtggccgcg
      841 ctcgatgcgt tcggactggc ggtcgccggc cgccaggcgc tcgacgccgg ggcctcgacc
      901 ggagggttca gccaggtgct tctggaacgc ggcgcctcgg tcgtggccgt cgacgtcggg
      961 cacggccaac tcgcgccgga gctcgtggac cacccgaggc tgcgcgccta cgacggcgtg
     1021 aacgtgcgca gcatcgacgc cgaggcatac gggcgcctca ccggcggcgc gagacgcccc
     1081 gacctcgtcg tcgccgacct ctccttcatc tccctcaccc aggtgattcc ggcactcgtc
     1141 gccaccgcgg cccgtgaggc cgacttcgtg cttctcgtga agccgcagtt cgaggtgggg
     1201 cgccgagggg tgccggccgg tgtcgtgcgc agcgcacggc tgcgcgaagg tgccgtcaac
     1261 ggggtcgtcg ccgcgctttt ccgcgcgggt ttgggtgtcg ccggcatcat cccgtcgccg
     1321 atcaccggcg agaaaggcaa ccgggagtat cttgtgcacg ccaccaggcg atgccgggcc
     1381 gatccgacag aatgggaggg gcggatccga gacatggtgg gaggacgatg acgaggccag
     1441 gcacgcggta catcctcgtc gtgctgcacg tcggccggga ggactccgtg cgggcgggcg
     1501 cgcacgtgtg ccggcagttg ctgggcgccg gtgccactcc cgtgctcacc tcggacggct
     1561 tggcggccct gcgccggctt gacccgggtc tcggcccgct ggccgagctg ggtgacgcgg
     1621 tgcacgcgga gcttctcgag cttgtgatcg tcttgggggg tgacgggacc attctgcggg
     1681 ccgccgaaat ggtgcgcggg taccgggcgc cgcttctcgg cgtgaacctc ggccacgtcg
     1741 gctttctcgc ggagagcgag cgtgacgacc tggacgaggc ggtgcggcgc ggcctcgccc
     1801 gcgactacga ggtcgaagag cggatgaccc tctccgtgcg ggtcatggtc ggcgacgatg
     1861 tcgtctacca gagttgggcg ctcaacgagg ccgcgatcga gaaggccagt cgggaacgga
     1921 tgctcgaggt cgtgatcgag gtgaatcgcc ggccgctttc cagtttcggc tgtgacgggg
     1981 tcgtgctctc cacgccgacc ggctcgaccg cgtacgcctt ctccggcgga ggaccgatcg
     2041 tctggcccac gctggaggcg ctccttctcg tgccgctcag cgcccacgcc ctcttcgcca
     2101 agcccttggt cgtcgacgcc ggggcggcgc tcgccgtcga gatactggaa cgcggcggcg
     2161 gcgccggcgt gctctggtgt gacgggcggc gtactttcga cctgccgccc ggcgcccgcg
     2221 tcgtcgtccg gcgctccccc acggcggtct gcctcgcccg actgcatcac ggtgaattca
     2281 ccgaccggct cgtgcgcaag ttcagcctgc ccgtcaccgg ttggcgcggc cccgccggcg
     2341 ccgactgacc cgccggccgg cccaccccga ggagagaggc agccatgatc gaggagatcg
     2401 gcatccatgg tctcggcgtc attgacgacg ccaccttgcc gctggcggcg gggttcaccg
     2461 cgctgaccgg cgagaccggc gccggcaaga cgatggtcgt ctccgcactc gggctgcttc
     2521 tcggccaacg cgccgactcg ggccttgtgc gcccgggctc cggcgtcagt gtggaaggcc
     2581 gttggctcgt gccgggcgac ggttacgtcg cctgccgggt gcgggaggcg ggcggtatcg
     2641 tcgacgacgc cgcggcggat ggcgcgggcg ccggcgaggc cggcgtggtc gtcacccgca
     2701 cgcttgccgc ttcgggccgc agcaaggcga gcatcgg
//
LOCUS       DASTNL010000228         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1095304, whole genome shotgun sequence.
ACCESSION   DASTNL010000228 DASTNL010000000
VERSION     DASTNL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095304"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..3684
                     /locus_tag="VFQ96_07300"
     CDS             4..3684
                     /locus_tag="VFQ96_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0257629.1"
                     /translation="MADTVGQGGQAGSVAALARTHPQNVSLPDPAVVPVNAAEPERER
                     WRAQLAQLGGTSPLLHFDDEPSTGIELSTTHPGGLPSLIMGEKTLLSSLIRDEIALHK
                     ARRGASRVATKDVELRSMRGINAVHLAIGLAKWQYGGENYCAPVLLRPLAIRHHGRDF
                     ELKLRGTTFVNPELVTALQKLFHIVLDAEELVALSTASGVFKPQPVIDRLRGVTAHLP
                     WFTVHPRLVVSTFADVGPAMLRDAARLDTRLLDALAGNQTARRDVKASFSPVASVPED
                     ERPPETDALLLDADDEQDDVIAQVAAGNSLAVRMLPGTGTTQTVVNTIGTLVTQNKTV
                     AVVSPRRSSLDDVSRALQRLGLTGMAVGPESLRRDLIRGIGRNEKAVQPQLREVDEAL
                     VRLRKVLLDYRHALTRTDPQLRVSVLDALQRLAGLAQQANPPATTARLERASVAALVT
                     GRDEAARTLAKAAELGQFRYGPEDSPWFAASFSSVAEATSSHDMAKRLHTFEMRQLLE
                     RAADVVGPTGMRPFLTMQELGVYLRLLLDVRESLDHFEPAIFDRPLTDMITATSNRRI
                     SSTLPRSHRRRMRRLAEEYLRPGVHVADLNESLQAVQRERTLWQRYATVGATPRVPAG
                     VTDLYVAYQRVAEDLAALDRPLGRTETPRSLLLLPIDALRRLVGELAADSQVLANLQE
                     RTALLAELGSLRLGPLLDDLAARHVEEGQVAAELELAWWQSAFEILVDGDTALLDANT
                     AVLDRLEADYRLVDQAHAEESGGKLAWQLAQRWRIGLRDYTDEASALRRLLSGPGPVS
                     AEALGAAAPHLGRQLAPVWLASPYEAAQLGAAARFDTVLIVDAGAVTVAEVAGVLRRS
                     DQVVAFGDPVIQTPSPFTIALGESAAEPALSDEAAPASAFDELADLLPTATLVRSYRA
                     GGEDLADLVNRTFYGDRIDCLPWAGTFLGHGSLTVHYVAGGHGMPDEVSGAVESTDAE
                     VEATVSMVLDHAAHHPRESLMVITASARHAVRVQQAVLAAFVRYEELADFILAERSEP
                     FTVVTLEQAMAQSRDRVIFSIGYGRTPHGRLLSSFGPLGRPGGERLLGIGMTRARRAM
                     NIVSCFRPEDIDQSRELHGVASLARLLEEVQAIERRGDEPDDANTMIADLARRLRRRR
                     LNVRVGYRGKLELVASFGRKAVVVETDETVATHSLRQSLRVRPDVLRRLGWHYLRVHD
                     FELFSDPEAVALRIARLIGAAE"
     gene            complement(3981..4460)
                     /gene="mscL"
                     /locus_tag="VFQ96_07305"
     CDS             complement(3981..4460)
                     /gene="mscL"
                     /locus_tag="VFQ96_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU0257630.1"
                     /translation="MIKGFKEFILRGNVLDLAIAVVIGAAFGAVVTSLVDKVFNPLIG
                     ALFNAEGLATSFPITIPTSSGGHATIYFGAVIAALINFLLVALLVYFVFVLPMNTYSK
                     RQKARRQAGEPEPEDTPVTELEMLTQIRDLLADVAVPGASRHTASKHTAEEPGSDPE"
     gene            complement(4533..4868)
                     /locus_tag="VFQ96_07310"
     CDS             complement(4533..4868)
                     /locus_tag="VFQ96_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU0257631.1"
                     /translation="MPTYSYKCTECGAAFDIHQEFTDDALTVCPTCGGRLRKVFSSIG
                     VTFNGPGFYSTDSRSASTSHDEPHTAPAKAEGASPTAGTAEKKPAGTGKKTEGAATKK
                     KAASPAPVS"
     gene            complement(4885..5553)
                     /locus_tag="VFQ96_07315"
     CDS             complement(4885..5553)
                     /locus_tag="VFQ96_07315"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU0257632.1"
                     /translation="MTVDTAEEKLALRSRIRAGRRAMPQEERQRAATALGERLRLLTL
                     AADATCVSAYLSSPSEPGTRDFLAWARAAGVQVLLPLSRPDGLLDWAYDADPDAGGGE
                     RMTTLGVPESTGTPLGPAAIERARLIFAPAAAVGSDGTRLGWGKGYFDRMLTGMRKCP
                     QVYAVVFDGELLARVPRELHDRAVDGVVTPSKTVRFDRPGLHRPRPRAHERRMPPPGD
                     PHAS"
     gene            5603..>6152
                     /locus_tag="VFQ96_07320"
     CDS             5603..>6152
                     /locus_tag="VFQ96_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0257633.1"
                     /translation="MAHEVTKAVIPAAGLGTRFLPATKAMPKEMLPVVDKPAIQYVVE
                     EAVAAGLHDVLMVTGRNKNALENHFDRNAELEDMLEKKGDTDRLEKVHYSTALADLHY
                     VRQGDPKGLGHAVLRAKMHVGREPFAVLLGDDIIDSRDDLLLHMLDVQQKYNATVIAL
                     MEVPSESIHLYGAAAIEETGDDD"
BASE COUNT          960 a         2102 c         2126 g          964 t
ORIGIN      
        1 ctcatggcgg acaccgtcgg tcagggcggc caggccggct ccgtcgccgc gctcgcacgc
       61 acacatccgc agaacgtgag tctgcccgac ccggccgtcg tgccggtcaa cgcggccgaa
      121 ccggaacgtg aacgttggcg agcccagctg gcgcagctgg gcggcacctc gccgctgttg
      181 catttcgacg acgaaccgtc aaccggtatc gagttgagca cgacccaccc ggggggcctg
      241 ccctcgctca tcatgggcga gaagacgctg ctctcgagcc tcatccgcga cgagatcgcg
      301 ttgcataagg cgaggcgcgg cgcgagccgg gtcgcgacga aagacgtcga gctgcgctcg
      361 atgcgcggca tcaacgccgt acacctcgcg atcgggctcg cgaagtggca gtacggcggc
      421 gagaactact gcgcgccggt gctgctgcgc cccctcgcca tccggcacca cggccgcgac
      481 ttcgagttga agctgcgcgg cacgacgttc gtgaaccccg agctcgtcac cgcattgcag
      541 aagctcttcc acatcgtgct cgacgcggag gagctcgtcg cgctctccac ggcgagcggc
      601 gtgttcaagc cccagccggt gatcgacagg ctacgtgggg tgacggccca cctgccctgg
      661 ttcaccgtgc atccgaggct cgtcgtctct accttcgccg atgtcggccc tgcgatgctg
      721 cgcgacgccg cccgacttga cacccgcctg ctcgacgcgt tggccggcaa ccagacggcc
      781 cggcgcgacg tcaaggcgtc tttctcgccg gtggccagcg tgccggaaga cgagcgcccg
      841 cccgagaccg atgcgctctt gctcgacgcc gacgacgagc aagacgacgt gatcgcccag
      901 gtcgcggccg gcaacagcct cgccgtgcgc atgctgcccg gcaccggcac gacgcagacc
      961 gtggtcaaca cgatcggcac gttggtgacg caaaacaaga cggtcgccgt cgtcagcccc
     1021 cggcgctcca gcctcgatga cgtgtctcgg gcgttgcaga ggctcggcct gaccggcatg
     1081 gcggtggggc cggagagctt gcgccgcgac ctcattcggg gcatcggccg caacgagaag
     1141 gctgtgcagc cacagctgag agaggtcgac gaggcgctcg ttcggctgcg caaggttctt
     1201 ctcgactatc ggcacgcgtt gacccgtacc gacccgcagt tgcgggtgag tgtcttagac
     1261 gcgctgcaac gcctggcggg gcttgcccag caggcgaatc cgcccgccac gacggctcgc
     1321 ctcgaacggg cctcggtggc ggcgctcgtg accggccgcg acgaggcggc acgcacgctg
     1381 gccaaggctg ccgagctcgg ccagttccgg tacgggccgg aggactcgcc ctggttcgcc
     1441 gcgtcgttct cctccgtcgc cgaggcgacc tcgtcgcacg acatggcgaa gcgcctgcac
     1501 acgttcgaaa tgcgccagct tctggaacgt gcggccgacg tcgtgggccc caccggcatg
     1561 cggcccttcc tgacgatgca agaactgggc gtctacctgc gcctgttgct cgacgttcgt
     1621 gagagcctcg accatttcga gccggccatc ttcgaccggc cgctgaccga catgatcacc
     1681 gccacctcga accggcgcat cagctcgacc ctgccgcgtt ctcatcgccg gcgcatgcgt
     1741 cgccttgccg aggagtacct gcggcccggc gtgcacgtcg ccgacctgaa cgagagcctg
     1801 caagccgtgc agcgggagcg caccttgtgg cagcgttacg cgacggtggg ggcgacaccc
     1861 cgggtgccgg ccggcgtcac cgacctctat gtcgcctatc agcgggtggc agaagatctc
     1921 gccgcgttgg atcgcccgct cgggcgtacc gagacgcccc gctccctgct gctgttgccg
     1981 atcgacgcgt tgcggcggtt ggtgggcgag ttggccgccg actctcaggt gcttgcgaac
     2041 ctgcaagaac gcaccgcgct tctggccgag ctcggcagcc tgcggctcgg cccgttgctc
     2101 gacgacttgg ccgcccggca cgtcgaggag gggcaggtcg ccgccgagct ggagttggcg
     2161 tggtggcagt cggcgttcga gattctggtc gacggcgaca ctgctctgct cgacgcgaat
     2221 accgcggtgc tcgaccgtct cgaggccgac tatcggctcg tcgatcaggc ccacgccgag
     2281 gaatcgggcg gcaagcttgc ctggcaactc gcccagcgtt ggcggatagg cctgcgcgac
     2341 tacaccgatg aggcgtccgc gttgcgccgg ctcttgagcg gtcccggccc ggtgagcgcg
     2401 gaggcgctgg gggccgcagc gcctcatctt ggtcggcagc tcgccccggt ctggctcgcc
     2461 tctccttacg aggcggccca gctcggggcc gcggcgcgct tcgacactgt gctcatcgtc
     2521 gacgctggcg ccgtcactgt cgccgaggtg gcgggcgtct tgcggcgaag cgaccaggtc
     2581 gtcgcgttcg gcgaccccgt catccagaca ccgtcaccct tcacgatcgc gctcggcgag
     2641 agcgcggccg agccggcgtt gagcgacgag gcggcgcccg cctcggcgtt cgacgagctg
     2701 gccgaccttt tgcccacggc gacgttggtg cgcagctacc gggcgggcgg cgaagacctc
     2761 gcagacctcg tcaaccgcac cttctacggc gaccgcatcg actgcctgcc ctgggcaggc
     2821 accttcctcg gccacggcag cctcaccgtg cattacgtgg ctggcggtca cggcatgccc
     2881 gacgaggtca gcggcgccgt ggagagcacc gacgccgagg tggaggcgac ggtgagcatg
     2941 gttctcgacc atgcggcgca tcatcctcgg gaatcactca tggtcatcac ggcgagtgct
     3001 cgccacgcgg tgcgcgtgca gcaggccgtg ttggccgcgt tcgtgcgtta cgaggagctc
     3061 gccgacttca tcctcgcgga acgctccgaa ccgttcaccg tcgtcacgct ggagcaggcc
     3121 atggcgcaaa gccgtgaccg cgtcatcttc tccatcggtt acggtcgcac gccgcacggc
     3181 cggctgcttt ccagcttcgg cccgctcggc cggccgggcg gtgaacgcct cctcggcatc
     3241 ggcatgacca gggcgcgccg cgcgatgaac atcgtgtctt gttttcgccc ggaagacatc
     3301 gaccagtctc gagagctgca cggcgtcgcg tcgctcgcac gcctgctcga agaggttcag
     3361 gcgatcgaac ggcgcggcga cgagccggac gatgcgaaca cgatgatcgc cgatctcgcc
     3421 cgcaggcttc ggcgccgacg cctgaacgtg cgcgtcggct atcgcggcaa gttggagctc
     3481 gtggcgtcgt tcggccgcaa ggcggtcgtc gtcgagacag acgagaccgt cgcgacgcac
     3541 agcctgcggc agtccctccg agtgcggccc gacgtgttgc gtcgcctcgg ctggcactac
     3601 ctgcgggtgc acgacttcga gttgttcagc gacccggagg cggtcgccct gcgcatcgcc
     3661 aggctgatcg gcgcggccga atgagcgacg aaggcgacaa gcacgagaag ccgagcccct
     3721 tgcggaatgg ggggtcgggg tctcgccgtc ggcatcggag ggccagccgg ccgggagctg
     3781 cagggtctga cccgcggccg agtctcggcc gggcggaacg tgccgcgttc gacacggatc
     3841 gggcctgggg cgacggcgag gacagcaacg accgcagtct tagagagaac gtgcctccgc
     3901 actggggcaa gcgctagggg tgctgaaccc tcgcctcgtc tcgtgagaga acgggcagag
     3961 cggcggcacg cgctccacgc tcactccggg tcgctgcccg gctcctcggc ggtgtgcttc
     4021 gacgccgtgt gccgggaggc gcccggtacc gcgacgtcgg ccagaaggtc gcggatctgc
     4081 gtcaacatct ccaactccgt caccggcgta tcttccggtt ccggctcgcc ggcctggcgg
     4141 cgggccttct gccgcttgga gtaggtgttc atcggcagca cgaagacgaa gtagacgagc
     4201 aaggcgacga ggaggaagtt gatgagtgcg gcgatgaccg cgccgaagta gatcgtcgcg
     4261 tgcccgcccg acgacgtcgg gatcgttatg gggaacgagg tcgcgagacc ttcggcgttg
     4321 aagagcgcgc cgatgagcgg gttgaagacc ttgtcgacga gagacgtgac gaccgcgccg
     4381 aacgccgcgc cgatgacgac ggcgatcgcc agatcgagca cgttgccgcg gagaatgaac
     4441 tccttgaacc ccttgatcac gatatccccc tcatgtcagt gaacgcgggc cggccggcca
     4501 atgacacacg gccgcgatgc tcgtttggtt tttcaggata ctggagcggg gctcgctgcc
     4561 ttcttcttcg tcgcggcacc ctctgttttc ttgcccgtgc cggccggttt cttctcggcc
     4621 gtgccggccg tgggggaggc accctcagcc ttggcgggtg cggtgtgcgg ctcgtcgtgg
     4681 ctggtcgagg ccgaacggga atcggtgctg tagaagccgg ggccgttgaa ggtgacgccg
     4741 atgctgctga aaaccttgcg aaggcggccg ccgcaggtgg ggcagacggt gagcgcatcg
     4801 tcggtgaact cttgatgaat gtcgaaggct gcaccgcatt cggtgcattt gtaggaatat
     4861 gtgggcactg ctgtctccag aaggtcagga cgcgtgcgga tcgccgggtg gcggcatccg
     4921 cctctcgtgt gcccggggac ggggtctatg aagaccgggt cggtcgaaac gcacggtttt
     4981 gctcggcgtg accaccccgt caaccgcacg atcgtgcagc tcccgaggga ctcgcgccag
     5041 aagctcgccg tcgaacacga cggcatacac ctggggacat tttctcatgc cggtgagcat
     5101 tctgtcgaag tagcccttgc cccagccgag tctggtgccg tctgaaccca ccgccgcggc
     5161 gggggcgaag atcaggcggg cacgctcgat cgccgccggg ccgagtgggg ttccggtgct
     5221 ctccggcacc ccgagcgtcg tcatcctctc gccgccgccc gcgtcggggt cggcgtcgta
     5281 cgcccagtcg agcaggccgt ctggccggga gagcggcagc agcacctgca cgcccgccgc
     5341 gcgagcccag gcgaggaaat cccgggtacc gggttcggag ggggaggaga ggtaggcgga
     5401 gacacaggtg gcgtcggccg ccagcgtgag gaggcgcaga cgctcgccga gcgccgtcgc
     5461 cgcccgttgc cgctcctcct gaggcatcgc ccgacggccg gcgcggatgc gggagcgcag
     5521 agcgagcttc tcttcggccg tatccacagt catgcccgaa aggctatccg gcccgtgacg
     5581 ctatacggat agggttagcc gcatggcaca cgaggtaacc aaggccgtca tccccgccgc
     5641 cggactcggc acccggttcc tgcccgccac gaaggcgatg ccgaaagaga tgctgccggt
     5701 cgtcgacaag ccggccatcc agtacgtggt cgaggaggcc gtcgccgccg ggctgcacga
     5761 cgtcctgatg gtgaccgggc gcaacaagaa cgcgctcgag aaccacttcg accgcaacgc
     5821 cgagctggag gacatgctcg agaagaaggg cgacaccgac cggctcgaga aggtccacta
     5881 ctcgaccgcc ctcgccgacc tccactacgt ccgccagggc gacccgaagg gtctcggcca
     5941 cgccgtgctc cgggccaaga tgcacgtcgg ccgcgagccg ttcgcggtgc tgctcggtga
     6001 cgacatcatc gactcgcgcg acgacctcct gctgcacatg ctcgacgtcc agcagaagta
     6061 caacgccacc gtgatcgcgc tgatggaggt gccgagcgag tccatccacc tgtacggcgc
     6121 cgccgcgatc gaggagaccg gcgacgacga tg
//
LOCUS       DASTNL010000229        10348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_726345, whole genome shotgun sequence.
ACCESSION   DASTNL010000229 DASTNL010000000
VERSION     DASTNL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726345"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VFQ96_07325"
     CDS             <1..1638
                     /locus_tag="VFQ96_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEU0257634.1"
                     /translation="RGRPRVRRPPSEWTRLDRWWLRFSSSRWRPWWDRAAPILILALA
                     AFTRLWRLGEPHSLVFDETYYVKDSWTLMHLGYEASWPQNADVQFNAGSVNGYAAGNP
                     EFVAHPPFGKWLISVGLAVFGAQNSVGWRISTAVLGILAVALVYVIAHRMFHSTLIAS
                     VAGLLMAVDGTAIVMSRVALLDNSVMFLALLAFWFVLIDRGWAARRLERALVGRAGHG
                     RSEPSMAGPEGARAAPAPRYGPALWWRPWLLAAAAAVGLASGTKWTGLYFLAFFACYV
                     VVVDMAARRRHGLRHWLSGAVAKQGPVTFLVMVPLALATYIATWAGWIATSGGYYRHW
                     AQAVGGAWQGTLAWVPLWFQNLWHYQVAMYDYSINLHVPHPYESGPLRWLFLWRPTSM
                     YYVQTAHGQAGCPAVQCAREITELGNPFIWWVGTACMAYLVYRLVRYRSWRAGAILTG
                     FGAGYLPWLLYINRTIFEFYTIAYEPYMILGIAAVAAIVIGRPADPPRRRRIGLRLVA
                     GYLGIVCAASIFFWPIWVAARIPLWYWNLHMWLPSWR"
     gene            1783..3069
                     /locus_tag="VFQ96_07330"
     CDS             1783..3069
                     /locus_tag="VFQ96_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEU0257635.1"
                     /translation="MADREYGFRTRAIHAGNIPDPVSGARALPIYQSSAFVFDDAADA
                     EARFSLRKYGNIYSRLSNPTVASVEERVASLEGGLGAVATASGLSAQFLVFASLAGAG
                     DNIVASANLYGGSVTQLDVTLRRLGVDTTFVASADLADYDAAITDKTKFVFAETIANP
                     SGEIADIEGLAEVAHSHDLPLIIDSTIATPYLNRPFEWGADVAMHSATKFLCGTGTIV
                     AGMIVESGRFDWESPKRPLFTEPMHTYGGLTWSGNFAEYAFLTRLRAEQLRDIGPVLD
                     PHAAFLLSLGIETLPQRMQAHVDNARIVAEWLEADPRVEKVNWASLPSHPHHDRAAKY
                     LPKGASSVFSFELKGGRAAGQTFIESVNLASHLANLGDAKTLVIHPASTTHGQLTDQQ
                     LIDAGVLPGVVRISVGIEDPEDIIYDLDQALAQADA"
     gene            3088..3672
                     /locus_tag="VFQ96_07335"
     CDS             3088..3672
                     /locus_tag="VFQ96_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0257636.1"
                     /translation="MSTATADTDLVTTEFANGLSCQIPAASPLARALRRARTWVGPDA
                     KARLAILRGAKSIAIVGASDKMNRASFFVETYLTQSTDFRLYFVNPNLTTILGRPVYP
                     SLADLPEVPDIVDVFRKPQDLKSVIDEAEAIGATTVWMQLGIWNEEAARYGESKGMTI
                     VMDRCVKVEHARFHGGLHLFGFDTGQISSHKAMR"
     gene            3678..3890
                     /locus_tag="VFQ96_07340"
     CDS             3678..3890
                     /locus_tag="VFQ96_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257637.1"
                     /translation="MSQPAHRDIAAILVTALRRLGKAGDAETAMKLGGEAWWAFQNDG
                     DADSAEAVNKVMHYLARLLPAPGQKT"
     gene            3901..4584
                     /locus_tag="VFQ96_07345"
     CDS             3901..4584
                     /locus_tag="VFQ96_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU0257638.1"
                     /translation="MDPVTDLVMHLMASPWVYPVLFALVVIDGFFPPVPSESVVVAAA
                     AVGIASGAPAPWLIVLVAALGAALGDNIAYWIGRRAGVRFRWMRRRRVRRMLARSGRA
                     LRHRTAVVILTARYIPVGRLAVNMTAGATRLSWRRFWPLTVLGGVSWSIYSTMVGLVA
                     GTWAHNQPLVAAAVGIVIAVALGLATDQVTTRVRQARRRHALRRRLSLRARRLPSLAS
                     AATPQGGAQ"
     gene            4595..5506
                     /locus_tag="VFQ96_07350"
     CDS             4595..5506
                     /locus_tag="VFQ96_07350"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0257639.1"
                     /translation="MTSTASPAGAAAPTLTVPSPAVSTNWLAEHLHDPGLVVADVSVL
                     KVDSPDGRYSFTSGDDPYVIDGHIPGAVYADLLHSFSDPNGRYGFPRPDAQAFEEAAA
                     GLGVDNDTALVLYDTSIGHWASRLWWLFRTFGHDNVAIVSGGLTKWKAEGRALETGWV
                     PPREGSSFTARPREEMWASQDDVRAVVSGEAPGVLLCGVPRREYIGQAGLRPRKGHIP
                     GSRSIPPGTLIDRATNALYDVDQIRTTFEPLLKPSDRAVLYCSSGVASSENALALALA
                     GHENVALYDGSLNEWVADEANPLTLDA"
     gene            complement(5544..7253)
                     /gene="dgt"
                     /locus_tag="VFQ96_07355"
     CDS             complement(5544..7253)
                     /gene="dgt"
                     /locus_tag="VFQ96_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEU0257640.1"
                     /translation="MGVEGVDGSVPTKAGPAGAGVPAAEAQPGVPGRARAVRERLTAT
                     RFFPSPQAARVRRVAEPDPRLSHVYDDEFRVDLERIRFSPYFSRLSAVTQVIPQAGSG
                     TVIHNRLTHTLKVTAVARSIAMGLRAASEDDRETIVHLGGCDPVVVQAAAAAHDLGHP
                     PFGHLGEHVLDRLARDRLGLADGFEGNAQTLRIVAALDTCDAAPSGLNLTAGVRAAVL
                     KYPWTRGRWRGLAGTTPAHLLPRGVGRAAAAGAAKFSCYVLEEQEMRQARAAYPAVAW
                     AQQTVECAIMDIADDIAYSVHDLDDFYRAGVLQRSVVAAEFDAWLAGSTRLAALGEDE
                     LAGRPRTPGYALEATWRAAFAKDGWIADKEAFVDSVRRVQEDLVDGLLDVPYDGGVAA
                     DHAVAGFTRRWIDRLSRSVVVERHPNVRGSHVRLTRAAWHDVVVLKFVHAHFVLHRPD
                     LAIYQRGHARVIETLVRAFADWLADPDDAPRVPRRLADLVDAATAAYADLAAAGSSAP
                     SAACGGAGRDRAVAGPATVEARGRGRAIVDYIASLTDAQALSAAALIGGGSVGSWEDG
                     RSL"
     gene            complement(7463..8281)
                     /locus_tag="VFQ96_07360"
     CDS             complement(7463..8281)
                     /locus_tag="VFQ96_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0257641.1"
                     /translation="MSTPTTITPVGERRLVGHVGLGQTLANTLTMAHRGLLKIKRTPE
                     QLIDVTVQPVLFTLMFTYLFGGAIAGDPLKYLPFFLPGILVQTVLTTSVVTGVQLRED
                     MDKGVFDRFKSMPIARIAPLSGALLADTVRYGIATVLTFLVGYIMGFRPGGGLGGVVV
                     AGVFVIVVSWALSWLFAFFGVIARTAASVQGISLVILFPLTFLSNAYVPTKTMPGWLQ
                     AFVNVNPISHLVTAARDLTTHGTAGIDLLWAIVGAAIVVAVFAPLTVRAYMRRA"
     gene            complement(8278..9285)
                     /locus_tag="VFQ96_07365"
     CDS             complement(8278..9285)
                     /locus_tag="VFQ96_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0257642.1"
                     /translation="MTTDPDWAVRAHGLVKTFGTNRAVDGVDLDVAAGSIYGVLGPNG
                     AGKTTTISMLATLLKPDAGEALVFGHDTQREAQVVRQLIGVTAQFASVDESMSAKENL
                     MLFCRLLGLSPAAARRRTADLLEEFALADVAKRTLRKFSGGMRRRLDLAASLIAQPPL
                     IFLDEPTTGLDPRTRAQMWQTIRRLVHTGSTVLLTTQYLDEADQLADRIAVIDTGRVV
                     AEGTADELKASVGTSSLQLRVADPGDLDVARRSLGTVLGTAAAVSGETTRLTVPLTDL
                     DKVVDLLILLREEGVRLAEVSVQKPTLDEVFLALTGHGAQPEAGGPADDLSREREGVH
                     A"
     gene            complement(9480..9977)
                     /locus_tag="VFQ96_07370"
     CDS             complement(9480..9977)
                     /locus_tag="VFQ96_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257643.1"
                     /translation="MAENPPMGGMPPGVRAIVLAAIEEATGRGAAAVEASDLLLALTI
                     GPGPVAEVLAEVGLDHDGLAGALRVEWEASLRYAGVAPLPAEQLAATPAHNRPRFGTS
                     AREALVAGHRMLAHAAARRSGRRRDDERSVALGVLHAELGTVPRALELAGVDRRSLAA
                     RIRAL"
     gene            complement(9970..10206)
                     /locus_tag="VFQ96_07375"
     CDS             complement(9970..10206)
                     /locus_tag="VFQ96_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0257644.1"
                     /translation="MTPAPPPALGGPADGLAAVVALRELADRLEDAEVERALREGWSW
                     SEVAAALGVTRQAVHKKHHRRLDAADIDLRRRHG"
BASE COUNT         1515 a         3693 c         3591 g         1549 t
ORIGIN      
        1 cggggccggc cgcgggtgcg ccggccaccg tcggaatgga cccgcctcga ccgctggtgg
       61 ctgcggttct cgtcgtccag gtggcggcct tggtgggatc gcgcagcgcc gatcctgatc
      121 ctcgcgctcg ccgccttcac gcggctttgg cgcctgggag agccgcacag tctcgtcttc
      181 gacgagacct attacgtcaa agactcgtgg accttgatgc acctcggcta tgaggccagc
      241 tggccgcaga acgcggacgt gcagttcaac gcgggcagcg tgaacggcta cgcggccggc
      301 aacccggagt tcgtggccca ccccccgttc ggcaagtggc tcatctcggt gggcctggcg
      361 gtcttcgggg cacagaacag cgtcggttgg cgcatcagca ccgcggtcct cggcatcctc
      421 gccgtcgccc tggtgtacgt gatcgcccac cgtatgttcc actccaccct catcgcttcc
      481 gtggccggcc ttctcatggc ggtggacggc acggcgatcg tcatgagccg cgtggcgctg
      541 ctcgacaact cggtcatgtt cctggcgctc ttggcgttct ggttcgtact catcgaccga
      601 ggctgggccg cacgccggct tgagcgtgcc ctcgtcggcc gtgccgggca cggtcggagc
      661 gaacccagca tggccgggcc ggagggcgcc cgcgcagcac ccgcccctcg ctacggcccg
      721 gccctgtggt ggcgaccgtg gctgcttgcc gccgcggccg ccgtcggcct cgcctccggc
      781 acgaaatgga cgggcctcta ctttctggcg ttcttcgcct gctacgtggt cgtcgtcgac
      841 atggccgccc ggcgccgcca cggcctgcgc cactggcttt ccggcgcggt cgcgaagcag
      901 ggtcccgtca cgttcctcgt catggtgccg ctcgcgctgg cgacctacat cgccacatgg
      961 gcgggctgga tcgccacctc cggcggctac taccggcact gggcgcaggc agtcggcggg
     1021 gcgtggcagg gcacgcttgc gtgggttcca ctgtggttcc aaaacctgtg gcactaccag
     1081 gtcgccatgt acgactacag catcaacctg cacgtgccgc acccctacga gtcggggccg
     1141 ctgcgttggc tcttcttgtg gcggccgaca agcatgtatt acgtgcagac cgcgcacggc
     1201 caggccgggt gccccgccgt gcagtgtgcc cgcgagatca cggagctcgg caacccgttc
     1261 atctggtggg tcggcaccgc gtgcatggcc tacctcgtct accggctggt gcggtaccgc
     1321 tcctggcgcg cgggcgcgat cctgaccggc ttcggcgccg gctacctgcc ctggctgctc
     1381 tatatcaacc gcacgatctt cgagttctac acgatcgcgt acgagcccta catgatcttg
     1441 gggatcgccg cggtggctgc gatcgtcatc ggccggcccg cggacccgcc gcgccgacgc
     1501 cggatcggcc ttcgcctcgt ggcgggctac ctcgggatcg tctgcgccgc cagcatcttc
     1561 ttctggccca tctgggtggc cgcgcgtatt ccgctgtggt attggaacct gcacatgtgg
     1621 ctgcccagct ggcgctgacc gggaaccagc ccggcagcag gcgaagggcg cgacggcggg
     1681 gtgcggcgat gggtcggcgc cggattgcgc catgttacgc atggccgggg gctcacgggc
     1741 ggcccgcacg gcgcctgacc gcggatcgac taccgtggaa acatggcaga tcgcgagtac
     1801 ggcttccgca cgcgggcaat ccacgccggc aatattcccg acccggtctc cggcgcgcgc
     1861 gccttgccga tctaccagtc gagcgcgttc gtgttcgacg acgccgccga cgcggaggcg
     1921 cggttctcgc tgcgcaaata cggcaacatc tactcacgcc tgtcgaaccc gaccgtggcg
     1981 agcgtcgagg agcgcgtcgc aagcctcgag ggcgggctcg gcgcggtcgc caccgcctcc
     2041 ggcctctccg cgcagtttct cgtcttcgcc tcgctcgcgg gcgccggcga caacatcgtc
     2101 gcctccgcca acctgtacgg cggctcggtg acccagctcg acgtgacgct gcgcaggctc
     2161 ggggtcgaca cgaccttcgt cgcctccgcc gatctcgccg actatgacgc ggcgatcacc
     2221 gacaagacca agttcgtctt cgccgagacc atcgcgaacc cgtcgggcga gatcgccgac
     2281 atcgagggcc tcgccgaggt cgcacattcc cacgacctgc cgctcatcat cgactcgacg
     2341 atcgccacgc cctacctcaa ccggccgttc gagtggggcg ccgacgtggc catgcattcc
     2401 gcgaccaagt tcctctgcgg caccggcacg atcgtcgccg gcatgatcgt cgagtcgggc
     2461 aggttcgact gggaaagccc gaagcgcccg ctcttcaccg agccgatgca cacctacggc
     2521 ggcctgacct ggtcgggcaa cttcgccgaa tacgcgttct tgacgaggct gcgcgccgag
     2581 cagttgcgcg acatcggccc ggtgctcgac ccgcacgccg cattcctcct ctcgctcggc
     2641 atcgagacgc taccgcaacg gatgcaggcg cacgtcgaca acgcacgcat cgtcgccgaa
     2701 tggctcgagg ccgacccgcg ggtcgagaag gtcaactggg cgagtcttcc gagccacccg
     2761 caccacgacc gcgcggcgaa gtacctgccc aagggcgcca gctcggtgtt cagcttcgag
     2821 ttgaagggcg gccgggccgc cgggcagacg ttcatcgagt cggtcaacct ggccagccac
     2881 ctcgcaaacc tcggcgacgc gaagacgctc gtcatccacc cggcctcgac gacgcacggc
     2941 cagctgaccg accagcagct catcgacgcg ggcgtcttgc ccggcgtggt gcgcatcagc
     3001 gtcggcatcg aagacccaga agacatcatc tacgatctgg accaagcgct cgcccaggcg
     3061 gacgcctagc tgcgggaggt aacaacaatg agcacggcca cggcagacac agacctcgtg
     3121 acgacggagt tcgcgaacgg gttgagttgt cagattccgg cggcgtcgcc gctcgcgcgg
     3181 gcgctgcgcc gagcgcgcac ctgggtggga cccgacgcga aggcccgcct ggcgatcctc
     3241 cggggggcga agtcgatcgc gatcgtcggc gcctccgaca agatgaaccg ggccagcttc
     3301 ttcgtcgaga cctacctgac ccagtccacc gactttcggc tgtacttcgt gaacccgaac
     3361 ctgacgacga tcctcggccg gccggtttac cccagcctcg ccgatctgcc ggaggtgccc
     3421 gacatcgtcg acgtgttccg caagccgcaa gacctcaaaa gcgtcatcga cgaggcggag
     3481 gcgatcggcg cgacgacggt ctggatgcag ctcggcatct ggaacgagga ggcggcccgc
     3541 tacggcgagt cgaaaggcat gacgatcgtc atggaccgct gcgtcaaggt cgagcatgcc
     3601 cgcttccacg gcggcctgca cctcttcggg ttcgacacgg gccagatttc cagccacaag
     3661 gcgatgcgct gacaggcatg tcacagcccg cacaccgcga catcgcggcc atcctggtga
     3721 cggcgttgcg tagactcggc aaagccggcg acgcagagac ggcgatgaaa ctcggcgggg
     3781 aagcctggtg ggcgtttcag aacgacgggg acgccgactc cgccgaagcg gtgaacaagg
     3841 tgatgcatta cctggcgcgg ctactgcctg cgcccggcca gaagacctga cgggagtcgt
     3901 atggatccgg tcaccgacct ggtcatgcac ctgatggcct cgccgtgggt gtatccggta
     3961 cttttcgcgc tcgtcgtcat cgacggcttc ttcccgcccg tgccgagcga gagcgtcgtc
     4021 gtcgcggcgg cggccgtggg catcgcctcc ggggcgcccg cgccgtggct catcgttctc
     4081 gtcgcggcgc tcggtgccgc gctcggcgac aacatcgcat actggatcgg ccggcgcgcg
     4141 ggcgtgcggt tccgctggat gcgacgccgc cgggtgcgac gcatgcttgc ccggtcgggt
     4201 cgggcgctca ggcatcgcac cgcggtcgtg atcctgacgg cgcgttacat tcccgtcggc
     4261 aggctcgccg tcaacatgac ggcgggggcg acgaggctgt cgtggcggcg gttctggccg
     4321 ctcacggtgt tgggcggcgt gtcctggtcg atctactcga ccatggtcgg cctcgtggcc
     4381 ggcacgtggg cgcacaacca gccgctcgtc gccgcggccg tgggtatcgt gatcgccgtc
     4441 gccttgggcc tggccaccga ccaggtgacg acccgggtgc gtcaggcccg acgccgccac
     4501 gcgctgcggc gacggctcag cctgcgcgcg cgccgcctgc cctccctggc ctccgcggcg
     4561 acgccgcagg gcggcgccca ataggcttga gagcgtgact tctaccgcct ccccagccgg
     4621 agccgccgcc ccgacactga cagtcccctc ccccgccgtc tcgacgaact ggctcgccga
     4681 gcacctgcac gaccccgggc tcgtcgtcgc cgacgtgtcg gtgctgaagg tcgacagccc
     4741 cgacggccgt tacagcttca cgagcggcga cgacccgtac gtcatcgacg gtcacatccc
     4801 gggcgcggtc tacgccgacc tgctgcattc cttcagcgac ccgaacgggc gatacggctt
     4861 tccccgcccc gacgcgcagg ccttcgaaga ggcggcggcg gggctcggcg tcgacaacga
     4921 caccgcactc gttctctacg acacctccat cggccactgg gcatcccgcc tatggtggct
     4981 gttccgcacc ttcggccacg acaacgtcgc gatcgtgagc gggggcctca ccaagtggaa
     5041 ggcggagggc cgtgccctcg agaccggctg ggtcccgccc agggagggct cgtcgttcac
     5101 ggctcggccc cgcgaagaga tgtgggccag ccaagacgac gtgcgcgccg ttgtgagcgg
     5161 cgaggcgccc ggcgtgctcc tgtgcggcgt gccccgtcgg gaatacatcg gccaggccgg
     5221 gctccgcccg cgcaaaggcc acattcccgg cagtcggagc attcccccgg gcacgctcat
     5281 cgaccgtgcg acgaacgcgc tgtacgacgt cgaccagatc cggaccacct tcgagccgct
     5341 cctgaagcct tccgaccggg cggtactcta ttgcagctcc ggcgtcgcct cgtcggagaa
     5401 cgcgctcgcc ctcgccctcg ccgggcatga gaacgtggcg ctctacgacg gctcgctcaa
     5461 cgagtgggtc gccgacgagg cgaatccgct caccctcgac gcctaggccg gccgccgccg
     5521 acgagcgcgc ccgggcaccg cggtcacagg ctccggccgt cttcccacga gccgacggag
     5581 ccgccgccga tgagcgccgc ggccgacaag gcctgagcgt cggtgagcga ggcgatgtag
     5641 tcgacgatgg cccggccgcg gcctcgtgct tcgacggtgg ccgggcctgc gactgcccgg
     5701 tcgcgtccgg ccccgccgca agcggccgac ggcgccgaag agccggccgc ggcgaggtcg
     5761 gcgtaggccg ccgtcgccgc gtcgacgagg tcggccagcc gacgcggcac gcgcggcgcg
     5821 tcgtcggggt cggcgagcca gtcggcgaag gcccggacga gggtctcgat gacccgcgcg
     5881 tggccgcgct ggtagatggc aaggtcgggc ctgtggagca cgaaatgtgc gtgcacgaac
     5941 ttcaagacga cgacgtcgtg ccacgcggcc cgggtcaggc gcacgtggga gccgcgcacg
     6001 ttcgggtggc gctcgaccac gacggagcgg ctgaggcgat cgatccagcg gcgcgtgaag
     6061 cccgcgaccg cgtgatcggc ggccacccct ccgtcgtagg gcacgtcgag cagcccgtcg
     6121 acgaggtctt cttgcacccg gcgcaccgag tcgacgaacg cctccttgtc ggcgatccag
     6181 ccgtctttgg cgaaggcggc ccgccaggtc gcctcgaggg catagccggg tgtgcgcggc
     6241 cgcccggcca actcgtcttc gcccagcgcg gccaggcgcg tgctgcccgc cagccacgcg
     6301 tcgaactcgg ccgcgacaac ggagcgttgc agcacgccgg cccgatagaa gtcgtcgagg
     6361 tcgtgtaccg aataggcgat gtcgtcggcg atgtccatga tcgcgcattc cacggtctgc
     6421 tgggcccagg cgaccgccgg ataggcggcc cgggcttgcc gcatctcttg ctcctccagc
     6481 acgtagcaag agaacttcgc cgccccagcg gccgccgcgc ggccgactcc gcggggcagg
     6541 aggtgcgcgg gggtcgtacc cgccaggcca cgccaacgcc cgcgcgtcca cgggtatttc
     6601 agcaccgcgg cacgtacccc cgcggtcagg ttgaggccgg agggagcggc gtcgcaggtg
     6661 tcgagagccg ccacgatgcg cagggtctgg gcgttgccct cgaagccgtc ggcgaggccc
     6721 agccggtctc gggcgagccg gtcgagcacg tgttcgccca ggtgcccgaa cggcgggtgg
     6781 ccgaggtcgt gtgcggcggc ggcggcctgc acgacgaccg ggtcacagcc tcccaggtgc
     6841 acgatcgtct cgcgatcgtc ctcactcgcc gcccgcagcc ccatcgcgat cgaccgcgcg
     6901 acggccgtga ccttgagggt gtgggtcagc cggttgtgga tgacggtgcc gctgccggcc
     6961 tgcgggatca cctgggtgac ggccgagagc ctcgagaagt acggcgagaa gcggatccgc
     7021 tccaggtcca cccggaactc gtcgtcgtac acgtgggaga gcctgggatc gggctcagcg
     7081 acgcgccgga cccgcgcggc ctgcggcgag gggaagaacc gtgtcgccgt gaggcgctcg
     7141 cgcaccgccc gggccctccc gggcacgccg ggctgcgcct cggccgccgg cacgccggcc
     7201 ccggccgggc cggctttcgt cggaacggat ccgtcgacgc cttcgacgcc catcgcgcct
     7261 ccccctcgtt cacgcctcca cgacgccccg gcacgcggcg cgccagcctc gaaggcccag
     7321 cccccgcgac atccggtgcg cctcccagca tggcaggcgc accggcccgg cggcgacccg
     7381 gcccgccgcc ggccggcgca cgggaaccag gcgccggccg gccgagcgcc ccggcggcgc
     7441 cccgccgagc gcgacgcgag cctcaggccc gtcgcatgta ggcccgcacg gtgagcgggg
     7501 cgaagaccgc gacgacgatc gccgctccga cgatcgccca aaggaggtcg atgcccgcgg
     7561 tgccgtgcgt cgtgagatcc cgggcagcgg tgacgagatg tgagatggga ttgacgttca
     7621 cgaacgcctg cagccacccc ggcatggtct tcgtgggcac gtaggcgttc gagaggaagg
     7681 tgagcgggaa gaggatgacg agcgatatcc cctgcacgct ggccgcggtg cgggcgatca
     7741 ccccgaagaa cgcgaacagc cagctgagcg cccacgagac gacgatcacg aagacgccgg
     7801 cgacgacgac gccgcccagc cctccgccgg gccgaaaacc catgatgtag ccgacgagga
     7861 aggtcagcac cgtcgcgatc ccgtaacgca ccgtgtctgc caagagagcc ccggacaacg
     7921 gtgcgatgcg ggcgatgggc atcgatttga accgatcgaa gacccccttg tccatgtctt
     7981 cgcgtagctg caccccggtg accaccgagg tggtgagcac cgtctgcacg aggatgcccg
     8041 gcaggaagaa cggcaggtat ttcagcgggt cgccggcgat cgcaccgccg aagaggtagg
     8101 tgaacatgag ggtgaacaac accggctgca cggtcacatc gatcagctgt tccggcgtgc
     8161 gcttgatctt cagaaggccg cgatgcgcca tggtcagcgt gttcgccaac gtctggccga
     8221 ggcccacgtg gccgacgagg cgccgttcgc cgaccggggt gatcgtcgtc ggtgtgctca
     8281 tgcgtgtact ccttcgcgtt cgcgcgagag atcgtcggcc gggccgcccg cctccggctg
     8341 cgcgccgtgg cccgtcaagg cgagaaagac ttcgtcgagg gtgggtttct gcacgctcac
     8401 ctcggcgagc ctcacgccct cctcgcgcag caggatgagg aggtccacga ctttgtcgag
     8461 gtcggtgagc ggcacggtca gcctggtcgt ctcgcccgag acggccgcgg cagtgccgag
     8521 caccgtgccg agcgagcgcc gtgccacgtc gaggtcgccc gggtcggcca cgcgcaactg
     8581 cagcgaggag gtgccgacgg aggccttcag ctcgtcggcg gtcccctccg ccacgacgcg
     8641 cccggtgtcg atgacggcga tccggtcggc cagctggtcg gcctcgtcga ggtactgggt
     8701 ggtgagaagc accgtggagc cggtgtgcac gaggcgacga atggtctgcc acatctgcgc
     8761 ccgggtgcgc gggtcgaggc cggtggtcgg ctcgtcgagg aagatcagcg gaggctgcgc
     8821 gatgaggctc gcggcgagat cgaggcggcg acgcatgccg ccggagaatt tgcgcagcgt
     8881 ccgtttcgcc acgtcggcga gcgcgaactc ttccaacagg tcggcggtgc ggcgtcgggc
     8941 cgcagccggg ctgaggccga gcaggcggca gaagagcatc agattctctt tcgcgctcat
     9001 cgactcgtcg accgacgcga actgggcggt caccccgatg agttgacgca cgacctgcgc
     9061 ctcacgttga gtgtcgtgcc cgaagacgag tgcttcgccc gcgtccggct tcaagagggt
     9121 cgcgagcatg ctgatcgtcg tcgtcttgcc ggcgccgttc gggccgagca cgccgtagat
     9181 gctgccggca gcgacgtcga ggtcgacgcc gtcgaccgcg cggttcgtgc cgaaggtctt
     9241 caccaggccg tgggcccgaa cggcccaatc ggggtcggtg gtcatggcgg tgtcctttcg
     9301 tatgtcgttt ccctgcgaat gccgcagggc ggcttgcgtg cagtgcatgc cggcgacgtt
     9361 cgcccggcgt gacgcagagg tcgtggtcgt ggatcgtggg gtggggggat gctcgtcgaa
     9421 cgaggcgaac cgccggccct gcgctgcccg cgatgggccg cctcggtagc atcctctctt
     9481 cagagagccc ggatgcgtgc ggcgaggctc cgccggtcga cccccgccaa ttccaaggcc
     9541 ctcggcacag tgccgagctc ggcgtgtaag acgcccaacg ccacgcttcg ctcgtcgtcg
     9601 cgccggcgac cgctgcggcg cgcggctgcg tgggcgagca tccggtgccc ggcgacgaga
     9661 gcctcgcgag cgctcgtgcc gaagcgcggc ctgttatgcg cgggggtggc ggccaactgt
     9721 tccgccggca gaggggccac gccggcgtag cgcaggctcg cctcccattc gacacgcaag
     9781 gccccggcga gaccgtcatg gtccaggccc acctcggcga ggacctcggc gaccgggccc
     9841 ggcccgatcg tcagggcaag cagcaggtcg gacgcctcga cggccgccgc cccgcggccg
     9901 gtcgcctcct caatcgcggc gaggacgatc gcccgcacgc cgggcggcat cccccccatg
     9961 ggcgggttct cagccatgac gcctcctgag gtcgatgtcg gcggcgtcga gccgccgatg
    10021 gtgtttcttg tgcacggcct gccgggtcac gccgagcgcc gcggcgacct cgctccacga
    10081 ccatccctcg cgaagggcac gctccacctc cgcgtcttcc agccggtcgg cgagctcgcg
    10141 cagggcgacg accgcggcga gcccgtctgc gggcccgccg agcgcaggtg gcggtgcggg
    10201 ggtcatgacg tcaacctacg ttgcttgagg caaactgtca aattaagttg cttatttaga
    10261 caccgaggtt gctagaacct ctctgcggcg gccggcgccg tcaggccgcc accgggcggt
    10321 caccgctcac cgatcgcggc ggcgccgc
//
LOCUS       DASTNL010000230         5104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_911920, whole genome shotgun sequence.
ACCESSION   DASTNL010000230 DASTNL010000000
VERSION     DASTNL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911920"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFQ96_07380"
     CDS             <1..438
                     /locus_tag="VFQ96_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0257645.1"
                     /translation="GHEVASIVEAASSARAALTGFSVAHAWRRRPRPVLADDLLPERA
                     LAGDPLARTALVQRIYLPLRARTQDLITLSAYLDSGRSLEATARELFVHPNTVRYRLR
                     RISEAIGWDATGPREALILQTALIVGAISEPAPGAEPPASDEP"
     gene            626..1546
                     /locus_tag="VFQ96_07385"
     CDS             626..1546
                     /locus_tag="VFQ96_07385"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU0257646.1"
                     /translation="MIVVACPGQGSQKAGFLAPWLGETRFAWHLAAISEACGIDLAMH
                     GTMSDNATITETSLAQPLIVAAGLLVLGELLADGRRAKIGGVAGHSVGEITAAAAAGI
                     LTEDDAVSFVRERGLAMAEAAGHDETGMSAVLGADETELERLLAQFGLAPANYNGGGQ
                     IVVAGPVKALAELKENPPAHARVIPLRVAGAFHTRFMLPAVQRLTAYAATLPAADPQM
                     RVWSNRDGQTVGDGRRFVDLMIGQVSSPVRWDRCMKSFVAAGITGLIEVCPAGALVGL
                     AKRGLPGIPTVAVKTPDDLPAAIELIDRDS"
     gene            1639..1887
                     /locus_tag="VFQ96_07390"
     CDS             1639..1887
                     /locus_tag="VFQ96_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0257647.1"
                     /translation="MALSSEDILAGLAILINDETGIATDTVEPGKSFTDDLDIDSISM
                     MTIVVNAEDKFGVKIPDDEVKNLKTVGDAVNYILKAQA"
     gene            1991..3235
                     /locus_tag="VFQ96_07395"
     CDS             1991..3235
                     /locus_tag="VFQ96_07395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0257648.1"
                     /translation="MTGKIVITGVGASSPLGGTAPESWEALLAGESGARTMEHDWVAK
                     YNLPVTFAAEAKVRPDTLLARQTARRLDPSSQFALVSAMEAWHDAGEPDVLPERLGVD
                     YATGIGGVWTLLDAWDTLRERGPRRVLPMTVPMLMPNAAAATLSMHFQARAFATTVAS
                     ACASSNESLVNAYEHLKAGLADVVIAGGTESAIHPITVASFASLQALSKRNDDPKTAS
                     RPYSVDRDGFVMGEGSASLVLETEEHALARGAHIYGELAGGAVTADSYHITANDPQGR
                     GARRAVLQALEVAGAGPDDVTHINAHATSTRVGDPAEYAALREVFGERVHEIPVSATK
                     ASTGHLLGGTGALEAVFTVFALRDRVAPPTINITTIDPEIPLSVSAATQPLGDGPQLA
                     ISDSFGFGGHNAVIALRDYPQA"
     gene            complement(3256..3762)
                     /locus_tag="VFQ96_07400"
     CDS             complement(3256..3762)
                     /locus_tag="VFQ96_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEU0257649.1"
                     /translation="MTVVAHGVLYVHSAPHAICPHVEWAAGRVLGRAIHFDWADQPAL
                     RGTRRAEFRWDGAPGAGATLASALRGWHDVRYEVTEDATAGMDGGRWLHTPRLGVFFA
                     QTDSVGNVVVPEDLLRHAMEGAGMNGAELRRRLEGALGQAWDDELEPFRYAGDACRSL
                     RVLHRVVG"
     gene            complement(3969..4844)
                     /locus_tag="VFQ96_07405"
     CDS             complement(3969..4844)
                     /locus_tag="VFQ96_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEU0257650.1"
                     /translation="MSDDLRRATLVSEGGGPAWKAQQELEAFHRRRTESVRTPQGFLA
                     LVNTQWITDRAAGPQSVWGAAGLWSPAAPGESGLRVTAQASDGVTVDGRLVEGEVVVR
                     GKDDPRPSTVVFGPAVTGYVIHGEEGYALRVWDHTSEANQRFGSIDAFPYNPDWVVEA
                     AFTPNPEGTTVGFEHVKSEGRTRELVVPGSITFTKDGIDYDVAAFQSGRALQLVFADA
                     TSGATTYGVGRFLFTAPRPDGSVPLDFNRAVLPPCAFSYSFNCPLPPAQNRFTVPVEA
                     GEKNVLDTEGRLLHA"
     gene            complement(4899..>5104)
                     /locus_tag="VFQ96_07410"
     CDS             complement(4899..>5104)
                     /locus_tag="VFQ96_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HEU0257651.1"
                     /translation="RDALRRGAHLVYFLHDELVVHTPEADAEQVAQIVRHAAWAAGRM
                     LFPASPATFPVTVDIVERYADAS"
BASE COUNT          826 a         1807 c         1755 g          716 t
ORIGIN      
        1 ggccacgagg tggcgagcat cgtcgaggcc gcgtcgagcg cccgcgcagc cttgaccggc
       61 ttctccgtgg cgcacgcctg gcgccgcagg cctaggccgg tcctcgccga cgacctgctg
      121 cccgagcggg cgctggccgg cgacccgctc gcgcgcaccg cgctcgtcca gcgcatctac
      181 ctgcccttgc gagcgagaac ccaagacctc atcaccctga gcgcctatct cgacagcggc
      241 cgctcactgg aggcgacagc gcgcgaactc ttcgtgcacc cgaacacggt gcgttatcgg
      301 ctgcgccgca tctccgaggc catcggatgg gacgcgacgg ggccgcggga ggccctcatc
      361 ttgcagaccg cgttgatcgt cggcgccatc agcgaaccgg cgcccggcgc cgagccgccc
      421 gcctctgacg agccgtgagt gcgtgacggc ccacgacact ccccggccgg gatggcgcgc
      481 gaccgtcgac gagcacgggc gatcggagcc gaccgacgat cggcgggtga cccgcgacta
      541 agttgtggca agaaaacaat tgcgagtcct gaaattagtc tgtgttccac accggtggcg
      601 cgagcctcct tggcagacta aagaggtgat cgtcgtcgca tgcccggggc agggatccca
      661 gaaggccgga ttcctcgccc cttggctcgg cgagacacgc ttcgcctggc atctggccgc
      721 gatctccgag gcctgtggta tcgaccttgc gatgcacggc acgatgagcg acaacgcgac
      781 gatcaccgag acctccctcg cccagcccct catcgtcgcg gccggccttc tcgtccttgg
      841 cgaactactg gccgacgggc ggcgggcgaa gatcggcgga gtcgcgggcc attcggtcgg
      901 cgagatcacg gcggcggccg cggccggcat cctcaccgaa gacgacgcgg tgtcgttcgt
      961 gcgagagcgg ggactcgcga tggcggaggc ggccggccac gacgagaccg gcatgagcgc
     1021 ggtactgggc gccgacgaga cggagctgga gcggctcttg gcacagttcg gcctcgcacc
     1081 ggccaactac aacggcggcg ggcagattgt cgtcgcaggc cccgtgaagg cactggccga
     1141 gctcaaagag aacccgccgg cgcacgcccg cgtcataccg ctgcgcgtgg cgggagcgtt
     1201 ccacacccga ttcatgctgc ccgccgtcca gcggctcacc gcctacgcgg cgacgctgcc
     1261 ggccgccgac ccgcagatgc gtgtctggag caaccgcgac gggcagaccg tcggggacgg
     1321 ccgccgattc gtcgacctca tgatcggcca ggtgtcctcg cccgtgcggt gggaccgctg
     1381 catgaagtcg ttcgtcgccg ccggcatcac cgggctcatc gaagtgtgcc cggccggggc
     1441 gctcgtcggg ctcgccaagc gcggcctgcc cggcatcccg accgtcgcgg tgaagacacc
     1501 cgacgatctg ccggccgcca tcgagctgat cgaccgcgac tcgtgaacgc cgaaggggcg
     1561 acgaccacgc ccaggcgtca cccgtcatcc ccgtaagata ttcaacgcct cacaccacac
     1621 aaacaggaga aatcaacaat ggccttgtcc tctgaagaca tcctcgccgg gctcgcgatc
     1681 ctcatcaacg acgaaaccgg catcgcgacc gacacggtcg agcccggcaa atcgttcacc
     1741 gacgacctcg acatcgactc gatctccatg atgaccatcg tcgtcaacgc cgaagacaag
     1801 ttcggcgtga agatccccga cgacgaagtc aagaacctga agaccgtggg cgacgccgtg
     1861 aactacatcc tcaaggcgca ggcctgaccc ggaacccgac cccgggatgc gccgcccgtg
     1921 cccgcggcgg aactcttgac ggcacgacag acacaacagc acagacagca acaacacgga
     1981 gtatcaccgc atgacaggaa aaatcgtcat cacgggtgtc ggggcatcct caccgctggg
     2041 cggaaccgcc ccggagagct gggaggctct gctggccggt gagtccggcg cccgcaccat
     2101 ggagcacgac tgggtggcga agtacaacct gccggtgacc ttcgcggccg aggcgaaggt
     2161 gcggcccgat accttgttgg cgcggcagac ggcgagacgg ctcgacccgt cgagccaatt
     2221 cgcgctcgtc tccgcgatgg aggcgtggca cgacgcgggc gagcccgacg tcctcccgga
     2281 gcgcctcggc gtcgactacg ccaccgggat cggcggcgtc tggaccctgc tcgacgcgtg
     2341 ggacacgttg cgggagcgcg ggccacgccg cgtcctgccg atgacggtgc cgatgctcat
     2401 gccgaacgcg gccgccgcga ccctgtcgat gcacttccag gcgcgcgcgt tcgcgacgac
     2461 cgtcgcctcg gcctgcgcgt cgagcaacga atccctcgtg aacgcctacg agcacctgaa
     2521 ggcggggctt gccgacgtcg tcatcgcggg cggcaccgag tccgccattc accccatcac
     2581 ggtggcgtcg ttcgcctcgc tgcaggcctt gtcgaagcgc aacgacgacc cgaagacggc
     2641 ctcccggccg tacagcgtcg accgggacgg gttcgtcatg ggcgaaggct ccgcaagcct
     2701 cgtgctcgag acggaagagc acgctctcgc gcgcggggcg cacatttacg gggagctggc
     2761 cggcggcgcg gtgacggccg actcgtacca catcaccgcc aacgatcccc agggccgcgg
     2821 agctcgccgt gcggttctgc aggcgctgga ggtggcgggg gccgggcccg acgacgtcac
     2881 ccacatcaac gcgcacgcga ccagcacgcg agtgggcgac ccggcggaat acgccgcgtt
     2941 gcgagaggtg ttcggcgagc gggttcacga gataccggtc tcggccacga aggcctccac
     3001 cgggcacctg ctcggcggca cgggcgcgtt agaggcggtc ttcaccgttt tcgcgctgcg
     3061 cgaccgcgtg gcgccgccca cgatcaacat cacgaccatc gacccagaga tcccgctgtc
     3121 ggtgtcggcg gccacccagc cgctgggcga cgggccccag ttggcgatca gcgactcttt
     3181 cgggttcggc ggccacaacg cggtcatcgc gttgcgcgat tacccgcaag cctgacaaag
     3241 gctcggcgac cctcgtcagc cgacgacgcg gtgcagcacg cgcaggctgc ggcacgcgtc
     3301 gccggcgtag cggaacggct cgagctcgtc gtcccaggct tgcccaaggg cgccttcgag
     3361 gcggcggcgc agttcggcgc cgttcatacc cgcgccctcc atcgcgtgcc gcagcaggtc
     3421 ttcggggacg acgacgttgc cgacgctgtc ggtctgggcg aagaacacgc ccagccgcgg
     3481 cgtgtggagc cagcggccgc cgtccattcc cgccgtggcg tcttcggtga cctcgtaacg
     3541 cacgtcgtgc cagccgcgca gggcggaggc cagggtggca ccggcgcccg gggcgccgtc
     3601 ccagcgaaac tcggcgcggc gcgtccccct gagtgcgggt tggtcggccc aatcgaaatg
     3661 gatggcgcga ccaagcacac gaccggccgc ccactcgacg tgcgggcaga tcgcgtgtgg
     3721 cgcggagtgc acatatagca ccccgtgggc gactactgtc atcgactcct ccgttctcgt
     3781 caggtgcgtc ttcccctacg acctgagagc agcggtagcc ggcgtgtagc gccgtatcaa
     3841 gttatctgtt cgcgcccatt atgcctcacc ggccggcgcc gatccatcgg cgcgtcgcgt
     3901 gcggtcgacg ccggggcgcc ggggtgggtc cactcgtgag gcgagaggcc gcgccggccc
     3961 gcgaggcctc acgcgtgcag cagccggccc tcggtgtcga gaacgttctt ctcccccgcc
     4021 tccaccggca ccgtgaaccg attctgcgcg ggcggcagcg ggcagttgaa gctgtagctg
     4081 aaggcgcagg gcggcaggac ggcgcggttg aaatcgagcg gcacggagcc gtcgggccgg
     4141 ggtgcggtga aaaggaaacg cccgacgccg taagtcgtcg caccgctcgt ggcgtcggcg
     4201 aagacgagct gcaacgcccg gcccgactgg aacgccgcca cgtcgtagtc aatgccgtct
     4261 ttggtgaacg tgatcgagcc ggggacgacg agttcgcggg tgcggccctc cgacttgacg
     4321 tgctcgaaac ccaccgtcgt gccctcgggg ttcggcgtga aggcggcctc cacgacccag
     4381 tcggggttgt aagggaaggc gtcgatcgat ccgaaccttt ggttcgcctc gctggtgtga
     4441 tcccacaccc gcagcgcgta tccctcctcg ccgtggatga cgtacccggt cacggccggg
     4501 ccgaagacga ccgtgctggg ccgcgggtcg tccttgccgc gcacgacgac ctcgccctcg
     4561 acgagcctgc cgtcgaccgt cacgccgtcg gaggcctgcg ccgtgacgcg caggccggat
     4621 tccccgggcg ccgccggcga ccacagcccg gccgcacccc acacgctctg cgggccggcc
     4681 gcgcggtcgg tgatccattg cgtgttcacg agcgcgagga aaccttgcgg ggtgcgcacc
     4741 gattcagtgc ggcgacgatg gaatgcctcg agctcctgct gggccttcca ggcgggcccg
     4801 ccgccttcgg aaacgagggt cgcacgccgc agatcgtcac tcacccgacc atgctctcac
     4861 ggcgacggcc ggggggtcgt gaggggctcc ggggtgggtc aggaggcgtc ggcgtagcgc
     4921 tcgacgatgt cgacggtgac cggaaaggtc gcggggctcg ccggaaagag catgcggccc
     4981 gccgcccagg ccgcatgtcg gacgatctgc gccacctgct ccgcgtcggc ctcgggtgtg
     5041 tgcacgacga gctcgtcgtg caagaagtag acgagatgcg cgccgcgacg gagggcgtcg
     5101 cggc
//
LOCUS       DASTNL010000231        11880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1650131, whole genome shotgun sequence.
ACCESSION   DASTNL010000231 DASTNL010000000
VERSION     DASTNL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650131"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1593
                     /gene="guaB"
                     /locus_tag="VFQ96_07415"
     CDS             85..1593
                     /gene="guaB"
                     /locus_tag="VFQ96_07415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU0257652.1"
                     /translation="MDQTDPFGFIGLTYDDVMLLPGHTNVIPSQADTTSRLSRRISLH
                     TPLISAAMDTVTEARMAVGMARNGGIGIIHRNLSIDDQATFVDKVKRSESGMITNPVT
                     TTPDATLADVDSLCAQFHVSGVPVIDAAGTLVGIVSNRDMRFVPPEQMATTRAGDVMT
                     PMPLVTAPVGVGHDEALALLARHKVEKLPLVDAAGVLKGLITVKDFDKSQRYPNATKD
                     DEGRLRVGAAIGFFGDAWQRAMTLVDAGVDVLVVDTANGDSQGVIDIIRRLKAEPRAA
                     HVDIIGGNVATAEGAKSLIAAGVDAVKVGVGPGSICTTRVVAGVGVPQVTAIYEAAKA
                     VASSGADVPVIADGGLQYSGDIAKALVAGAETVMLGSLLAGCDESPGDLVFEGGKQYK
                     KYRGMGSLGALQTRGKKTSYSRDRYFQADVPGDEQLIPEGIEGQVPYRGPLSAVVYQL
                     AGGLRQSMFYVGARTIPELRERGRFVRITAAGLKESHPHDIQMVVEAPNYRR"
     gene            complement(1614..2765)
                     /locus_tag="VFQ96_07420"
     CDS             complement(1614..2765)
                     /locus_tag="VFQ96_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0257653.1"
                     /translation="MVLSIPPLSTLRERTSSKWKTYPADVLPMFVAELDVALADPIRR
                     ALEKAVRDGDTGYSGPDRSLAEAFASFAAWRWGWSVDPGRVTYTTDVSVVLVESLRML
                     TRPGDGVIVMPPIYPPFFDLPPEAGARTVSVPLQKSGGSWRIDLAGIEREMAHGARVV
                     LLCHPHNPLGVVFSREELTELSEIVARHGGYVVSDEVHAPMTHADTAFVPYLTVSEAA
                     REHGVAAHSASKSWNVAGLKCAQFVTASARMDHLLRGMADEVHFRTGIFGRIAARAAY
                     TDARGWLDELDTFLGENRRLLARLVTERLPQVGYSEPHAGYLAWLDLSALGWGDDPAR
                     QALRLARVALNSGPTFGPQGAGHVRLNFGCAPETLAEGVGRLAEAARQR"
     gene            2835..3944
                     /locus_tag="VFQ96_07425"
     CDS             2835..3944
                     /locus_tag="VFQ96_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEU0257654.1"
                     /translation="MEIEIGRAKRARSVYSFDDVAVVPSRRTRDPEDVSVAWAIDAYH
                     FDIPILAAPMDSVVSPKTAIQIGRLGGLGVLDLEGLWTRYEDPEPVLATIRGLPPEGA
                     TAQLQRLYAEPIKPELITARIAEVRASGVTVAAALSPQRTQDFYRTVVEAGVDLFVIR
                     GTTVSAEHVSKNREPLDLKKFIYELDVPVIVGGAATYTAALHLMRTGAAGVLVGFGGG
                     AASTTRATLGIDAPMATAVADVAGARRDYLDESGGRYVHVIADGGLGTSGDVVKAIAC
                     GADAVMLGTALARATDAPGRGWHWGAEAYHGQLPRGNRVHVGQVASLEGVLFGPASVT
                     DGTANLVGALRRSMATTGYSSLKEFQRVEVVVGAH"
     gene            4168..5895
                     /locus_tag="VFQ96_07430"
     CDS             4168..5895
                     /locus_tag="VFQ96_07430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU0257655.1"
                     /translation="MSALETVVYPSKLGPEERRLAIEAMKERELDILVVGGGIVGTGC
                     AMDAATRGLSVGLVERRDFASGTSSRSSKLVHGGIRYLEQLDFRLVREALTERGLLLQ
                     RIAPHLVRPVRFLYPLKTRVAERFYIGSGMAIYDIFSYTGGMTPGVPHHRHLSAGQVH
                     RLAPGLSRSSYIGGITYYDAQVDDARYVASLARTAAHYGAHVATCVRVEGFLKVGERV
                     VGVKARDVLADETFEIRAKQVVNATGVWTDDTQAMVGERGQFKVRASKGIHLVVPRDR
                     FQSKSGLILRTEKSVLFVIPWGRHWLIGTTDTDWHLDKTHPAATAYDIDYLLEHVNAV
                     LTVPLTREDVEGVYAGLRPLLAGESEETSKLSREHMVAHSVPGLVVIAGGKWTTYRVM
                     AKDAIDAAVASMDGTIPESVTKNIPLVGAEGYRAAWNRRGKLAERVGIHPARIEHLLN
                     RYGTLTEDLVELIEGDPALGEPLPGADDYLGAEVVYACTHEAAQHLDDVLARRTRISI
                     EAWDRGVAAAPVAAALMAHSLGWSEERREREVAYYLARVEAERASQEQPDDESAEKAR
                     LTTPELPVG"
     gene            5936..6475
                     /locus_tag="VFQ96_07435"
     CDS             5936..6475
                     /locus_tag="VFQ96_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEU0257656.1"
                     /translation="MVDVRRVKLPGVGVLHSFVTEDGGKVGVIAHRSGHSDLITFSDE
                     REGGDITKVSLRLSEDEAHTLAELLGGTRITESLTGLDQVPGLSIDWFSVDYEDHIAG
                     QTLGRPADHGLVGLTVVAVVRGDSANPAPSPDFKIFPGDTLVVAGSPDKVAKAFTFFR
                     TGELPTATSAIPIDAPPGR"
     gene            6478..7809
                     /locus_tag="VFQ96_07440"
     CDS             6478..7809
                     /locus_tag="VFQ96_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0257657.1"
                     /translation="MIVGTDLIILGILLLVAYVLGRAGRLVGLPAIPIYMAVGLLCSP
                     HTTWFPLNFDPGHIELIAIVGLVLLLFSLGLEFDQDEFFGNAGKLLVTGGFYIVLNIG
                     VGFVFGMLVGWGPREALVIAGMVGTSSSAIITKLLIELRRLANDETPMILGVTVVGDI
                     FIAVYLAIVSVVLSGKTEVWPVVVQLTTAFCFLVAMFGVARFGGKVVSRLLRTKDEEL
                     FTILFFGLALLFAGIGEVIGVSDAIGAFLIGLVLGATTFHARLERVTVPLRDIFAAFF
                     FVNFGLGLNVTEFPAVVGIVLLAVLMTFVLNIVSGVAIAKMYGFHLPEGVNAAAILVN
                     RGEFTLILATLSVAAGLNGLLSAFAGLYVLVMAVLGPLFAANSERLGALLRRTRAAPR
                     AAVPNPVRDEEIALVDAVTDDDPERTQRAIDRMVEQAMMGEGVEGEEGDDH"
     gene            7844..8692
                     /locus_tag="VFQ96_07445"
     CDS             7844..8692
                     /locus_tag="VFQ96_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEU0257658.1"
                     /translation="MGGVTEVTLFRTMLRPRWIVALILAMAVAAGFAYLGKWQLGRAI
                     QSVEPAFAPTEQVKPFVGEARPGKDVTDASIGQRVSLQGSFVPGDYQLVADRLNHGRT
                     GYWVVGHLTLEETSASGQAEAVAVARGWAPTKSKAQAAARELSSRPASPVELTGRMLP
                     TEAPVVPPAGSAPKTMTMLAVADLVNRWHGMDDAVVFTAYVVEHGAVAGLDEIYSPPP
                     IVQHSLDLLNVFYAIEWAVFAGFALYLWYRVVKDYWERENGDETGVVRVGLASPGRPG
                     DGQKVD"
     gene            8697..9251
                     /locus_tag="VFQ96_07450"
     CDS             8697..9251
                     /locus_tag="VFQ96_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEU0257659.1"
                     /translation="MAYTPGSDEDASLLARILAQLRSADSARPRRIDLPKIPGALRLY
                     RVTAYVTGTMLLLLVAEMIVKYGVGYSLYFGVPGRPGFSFVEFNPLQTNLHGVDVSTA
                     ILIVHGWLYVVYLFADFRLWSLMRLSFTKFIQIALGGVVPFMSFIVEYFITRQVRRFL
                     ASVRQEDEARASAVDVHAAGEETH"
     gene            9220..10842
                     /gene="guaA"
                     /locus_tag="VFQ96_07455"
     CDS             9220..10842
                     /gene="guaA"
                     /locus_tag="VFQ96_07455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU0257660.1"
                     /translation="MCTPRGRRPISGASAAAASSATVLVVDFGAQYAQLIARRVREAH
                     AYSEIVPHTVTAAEIQRRHPAGIILSGGPASVYAPDAPGFDSGIFDLGVPVLGICYGF
                     QLMASALGGTVEHTGRREYGSTDVTVNEAGCQGVLLAGQPQAQRAWMSHGDSVTGAPK
                     DFAVLASSPATPVAAFANPRRGLYGVQWHPEVRHSDQGQRVLENFVHEVAGVGSEWTS
                     GNVIDDQVAAIRAQVGTGRVICGLSGGVDSAVAAALVHRAVGDQLTCVFVDHGLLRRD
                     ERIQVERDYVASTGVRLVTIDAADRFLAALEGVTDPEDKRKIIGREFIRTFEAAQARL
                     VAEAHDAGDPVRFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDLAFELVEPLRT
                     LFKDEVRAIGRALGLPEVIVSRQPFPGPGLGIRIVGAVTAGRLATLRAADAIAREELT
                     GAGLDGEIWQCPVVLLADVHSVGVQGDARTYGHPVVLRPVTSEDAMTADWSRLPYDVL
                     SRISNRITNEVEGINRVVLDVTSKPPGTIEWE"
     gene            10865..>11880
                     /locus_tag="VFQ96_07460"
     CDS             10865..>11880
                     /locus_tag="VFQ96_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0257661.1"
                     /translation="MDEVVARLLAALPDDRVRQNAEPRGRSAEISPSERSGSACLVDT
                     TRAAIAARSSDRSGLAGEAGARVVVRPRTTAEVARVMRIADETRTPVVPQGRLTGLAG
                     GANAIAGAILLDVSGMDRILRVDREELAAVVQPGVIVADLQKAAREHGLFYAPDPASA
                     RIASIGGTVATNAGGMRAVKYGVTRDWVRSLEVVTADGSVVRTRPQTVKAVAGYDLTG
                     LVVGSEGTLAVVTEATVRLIPAPAAPTGIAATFRDVPAALGAVAGIITGGALPATIEL
                     LDEASIRAIRRYAADGDDPEARFAAEALPEGAGAWIVVATDTPTAAADLRRYEHACRE
                     NGAQT"
BASE COUNT         1796 a         4042 c         4168 g         1874 t
ORIGIN      
        1 tgtggatgac atgcccgcgc cggccgtttg cgacggtgcc gctacgatgg gttcgaagaa
       61 gagtggcgat tcacaagggg tgacatggat caaacagatc cgttcggctt catcggtttg
      121 acctacgacg acgtgatgct gctccccggt cacaccaacg tgatccccag ccaggccgac
      181 acgacctccc gcctctcccg gcgcatcagc ctgcacaccc cgctcatctc ggccgcgatg
      241 gacacggtga ccgaggcgcg gatggccgtc ggcatggcac gcaacggcgg catcggcatc
      301 attcaccgca acctctccat cgacgaccag gcgacattcg tcgacaaggt gaagcgcagc
      361 gaatccggca tgatcacgaa cccggtgacc acgacccccg acgcgactct cgccgacgtg
      421 gactcgctgt gcgcgcagtt ccatgtgagt ggcgttccgg tcatcgatgc ggcaggcacg
      481 ctcgtcggca tcgtgtcgaa ccgcgacatg cgcttcgtgc cgccggagca gatggccacg
      541 acgcgggcgg gcgacgtcat gaccccgatg cctctcgtga cggctccggt cggcgtcggg
      601 cacgacgagg cactcgcgct tctggccagg cacaaagtgg aaaagctgcc gctcgttgac
      661 gcggccggtg tgctgaaggg cctcatcacg gtcaaagact tcgacaagtc gcagcgttac
      721 ccgaacgcga cgaaagacga cgagggtcgg cttcgggtcg gcgccgcgat cggcttcttc
      781 ggtgacgcct ggcagcgggc gatgaccctc gtcgacgcgg gggtcgacgt gctcgtcgtc
      841 gacaccgcca acggcgacag ccagggcgtc atcgacatca tccgccgctt gaaggcggag
      901 ccgcgggcgg cgcacgtcga catcatcggc ggcaacgtcg ccaccgcaga aggcgccaag
      961 tcgctcatcg ccgccggagt ggatgcggtc aaggtggggg tcgggcccgg atcgatctgc
     1021 acgacgcggg tcgtggcggg tgtcggggtg ccgcaagtca ccgcgatcta cgaggcggcg
     1081 aaggcggttg cgtcgagcgg cgccgacgtg ccggtgatcg ccgacggcgg gctgcagtat
     1141 tccggcgaca tcgccaaggc gctcgtcgcc ggcgccgaga cggtcatgct tggctcgctc
     1201 ctggccggct gcgacgagag cccgggtgac ctcgtgttcg agggcggcaa gcagtacaag
     1261 aagtaccgcg gcatgggctc cctcggggcg ttgcagacca ggggcaagaa gacctcatac
     1321 tctcgcgatc ggtatttcca ggcggacgtg ccgggtgacg agcagttgat ccccgaaggt
     1381 atcgaggggc aggtgccgta tcgcggcccg ctctcggccg tcgtctacca gcttgccgga
     1441 gggctgcggc agtcgatgtt ctacgtcggc gcccgcacga ttcccgagtt gcgtgagcgc
     1501 ggccggttcg tgcgcatcac ggcggccggg ctgaaggaat cacacccgca cgacatccag
     1561 atggtggtcg aggcacccaa ctaccgtcgc tgacgctgag cgcgtgggcc cggctatcgt
     1621 tgccgggccg cctcggccag ccgcccgaca ccctcggcga gggtctcggg cgcacagccg
     1681 aagttcaagc gcacgtggcc ggcgccctgc gggccgaagg tcggcccgct gttgagcgcg
     1741 acgcgtgcca ggcgcagcgc ctgacgcgcg gggtcgtccc cccaacccag ggccgagagg
     1801 tcgagccagg cgaggtagcc ggcgtgcggc tccgaatagc ccacctgcgg cagccgttcg
     1861 gtgacgagac gcgccagaag ccgtcggttc tcgcccagga acgtgtcgag ctcgtcgagc
     1921 cagccgcgag cgtcggtgta ggccgcccgg gcggcgatgc ggccgaagat gccggtgcgg
     1981 aaatgcacct cgtcggccat gcctcgaagc aggtggtcca tgcgggccga cgcggtgacg
     2041 aactgggcgc atttcagccc ggcgacgttc cacgacttgc tggccgagtg ggcggcgacg
     2101 ccgtgctcgc gagccgcctc cgacaccgtc agatagggca cgaacgcggt gtcggcgtgc
     2161 gtcatcgggg catgcacctc gtcgcttacg acgtacccgc cgtgacgggc gacgatctcg
     2221 gagagttccg tcagctcttc gcgcgagaag acgacgccca gcgggttgtg cgggtggcag
     2281 agcagcacga cccgggcacc gtgcgccatc tcgcgctcga tgccggcgag gtcgatccgc
     2341 cacgagccgc cactcttctg aagaggcacg ctcaccgtgc gggcgcccgc ctccgggggc
     2401 aggtcgaaga acggagggta gatgggaggc atcacgatca cgccgtcgcc ggggcgggtg
     2461 agcatgcgca gcgattcgac gagcacgacg ctgacgtcgg tggtgtaggt cacacggccg
     2521 gggtcgaccg accagcccca gcgccacgcc gcgaacgagg cgaacgcctc cgccaggctg
     2581 cggtcgggcc cggagtaacc cgtgtccccg tcgcgcaccg ccttctccag cgcccggcgg
     2641 atcgggtcgg cgagcgccac gtcgagttcg gcgacgaaca tcggcaggac atccgccggg
     2701 taggtcttcc atttcgagct ggttcgttcg cgaagcgtgg aaaggggcgg gatgctcagc
     2761 accatggcac ctagcatgcc gcatcgggtg caccttgggc cccgggcggc gcgggatagg
     2821 ctgggcgggt gagcatggag atcgagatcg gccgcgcgaa gcgggcgcgt agcgtgtact
     2881 cgttcgacga cgtcgcggtc gtgccgagca ggcgcacccg agaccccgag gacgtctccg
     2941 tggcgtgggc gatcgacgct taccacttcg acattccgat tctcgccgcg ccgatggact
     3001 cggtggtgag ccccaagacc gcgatccaga tcggccggct cggcgggctg ggggtgctcg
     3061 atctcgaggg actgtggacc cggtatgagg atccggagcc ggtgctcgcc acgatccggg
     3121 gtctgccgcc cgaaggcgcc acggcgcagc tgcagcggtt gtacgcggag ccgatcaagc
     3181 cggagctcat caccgccagg atcgccgagg tgcgcgcctc cggcgtcacg gtcgcggccg
     3241 ccctttcacc gcaacgcacc caagacttct atcgcaccgt cgtcgaggcg ggcgtcgacc
     3301 tgttcgtcat ccgcggcacg accgtgtcgg ccgagcacgt ctcgaagaat cgggagccgc
     3361 tcgatctcaa gaagttcatc tacgaactcg acgtgccggt catcgtcggc ggtgccgcca
     3421 cgtacacggc ggcgctgcac ctgatgcgca ccggcgcggc cggggttctc gtcggcttcg
     3481 gcggcggcgc cgcctcgacg accagggcca cactcggcat cgatgcgccg atggcgaccg
     3541 cggtcgccga cgtggcgggc gcccggcgcg actacctcga cgagtccggc ggccgctacg
     3601 tgcacgtcat cgccgacggc gggctcggca cctccggcga cgtcgtcaaa gcgatcgcct
     3661 gcggggccga tgctgtcatg ctcggcaccg ccctggcacg tgcgacagac gcgcccggcc
     3721 ggggttggca ttggggcgcg gaagcctacc acggccagtt gccacgcggc aaccgggtgc
     3781 acgtcggcca ggtcgcgtcg ctcgagggcg tgctcttcgg cccggcgtcg gtgacggacg
     3841 gcaccgccaa cctcgtcggc gcgctgcgcc gttcgatggc gacgaccggc tattcgagcc
     3901 tcaaggagtt tcagcgcgtc gaggtcgtcg tcggcgcaca ctgaatcgcc ttccgcgggt
     3961 cgcgggcgct ctcgggtgcc tcggacgctt cgagcccgac gggtcggtgc cgcccggcct
     4021 tgggcgcggg ccgggcgggg cgtcgcacgc gccgcggcag gcgaagagtg cgggggagac
     4081 gggcgcagca tagtctctaa agagacggtc cgggtgtgac gcggcagcca ccgtggcggt
     4141 gaggtcccgg ccgggtggga ggctgtgatg agcgcgttgg agaccgtcgt ctacccgtcg
     4201 aagcttgggc cggaagagcg ccgcctcgcg atcgaggcga tgaaagagcg cgagctcgac
     4261 atcctcgtcg tcggcggcgg catcgtcggc accgggtgcg cgatggatgc cgccacccgc
     4321 gggctctcgg tgggactcgt ggagcggcgt gacttcgcct ccggaacctc cagccgctcg
     4381 tcgaagctgg tgcacggcgg catccgctac ctcgagcagc tcgactttcg gctcgtgcgc
     4441 gaggcgctga ccgaacgggg gctgctgctg cagcgcatcg ccccccatct cgtgcggccc
     4501 gtgcgcttcc tttaccccct gaagacccgc gtcgccgaac gcttctacat cggcagcggc
     4561 atggcgatct acgacatctt ctcgtacacc gggggcatga ccccgggcgt gccccaccac
     4621 cggcacctct ccgccgggca ggtgcacagg ctcgccccag ggctctcgcg cagcagttac
     4681 atcggcggca tcacctacta cgacgcgcag gtcgacgacg cccgctatgt cgcgagcctc
     4741 gcccgcacgg ccgcacatta cggggcgcat gtggccacgt gcgtgcgggt cgagggcttc
     4801 ctcaaggtgg gcgagcgcgt ggtcggcgtc aaggcccgcg acgtgctcgc cgacgagacc
     4861 ttcgagatcc gcgccaagca ggtcgtcaat gcgacgggcg tgtggaccga cgacacgcag
     4921 gccatggtgg gcgagcgcgg ccagttcaag gtgcgcgcct ccaagggcat ccatctcgtc
     4981 gtgccgcgcg accgcttcca gtcgaagtcg ggcctcatct tgcgcaccga gaagagcgtg
     5041 ctcttcgtca tcccgtgggg caggcattgg ctgatcggca cgaccgacac cgactggcac
     5101 ctcgacaaga cgcatcctgc agccacggcc tacgacatcg actacctgct ggagcacgtc
     5161 aacgccgtct tgacggtgcc gctcacccgt gaggacgtgg agggcgtcta cgccgggctg
     5221 cggccgcttc tggcaggcga gtcagaagag acctccaagc tttcccgtga gcacatggtg
     5281 gcgcattccg tcccgggcct cgtcgtcatc gcgggcggca aatggacgac gtatcgcgtc
     5341 atggcgaaag acgcgatcga cgcggccgtc gcgtcgatgg acggcacgat cccggagtcg
     5401 gtgacgaaga atatcccgct cgtcggcgcc gagggctatc gggcggcgtg gaacaggcgc
     5461 ggcaagctcg ccgaacgggt cggcatccac ccggcgcgca tcgaacacct cttgaaccgg
     5521 tacggcacgc tcaccgaaga cctcgtcgag ctgatcgagg gcgacccggc actcggcgag
     5581 ccactgccgg gcgccgacga ctacctcggc gcagaggtgg tctacgcctg cacgcacgag
     5641 gcggcacagc acctcgacga cgtgctcgcg aggcgcaccc gcatctcgat cgaggcgtgg
     5701 gatcgcggcg tggccgcggc gcccgtggcc gcggccctca tggcgcactc gctcggctgg
     5761 agcgaggagc gccgcgagcg ggaggtcgcg tattatctcg cccgtgtcga ggccgaacgt
     5821 gccagccagg agcagcccga cgacgagtcg gccgagaagg cccggctgac gactcccgag
     5881 ctgccggtcg gctagcccgt aggattgagg gaccgacgaa ccgaggagcc tcacgatggt
     5941 cgatgtacgc cgggtcaagc tgcccggggt cggggtgctg cacagtttcg tcaccgaaga
     6001 cggcggcaag gtcggcgtca tcgcccatcg ttccggccat agcgacctca tcacgttctc
     6061 tgacgagcgc gagggcggcg acatcacgaa ggtgtcgttg cggctgagcg aagacgaggc
     6121 gcataccctc gcggagcttc tgggcggcac ccgcatcacg gagtcgctca ccgggctcga
     6181 tcaggttccc ggcctgtcga tcgactggtt cagcgtcgat tacgaagacc atatcgccgg
     6241 ccagacgctc ggccggcctg ccgatcacgg gctcgtcggt ctcacggtcg tcgccgtcgt
     6301 gcgcggcgac tccgccaacc cggcaccctc ccccgacttc aagatcttcc caggcgacac
     6361 cctggtcgtc gcgggcagcc cggacaaggt ggcgaaagcc ttcaccttct ttcgcaccgg
     6421 cgagctgccg accgcgacct cggcgatccc gatcgacgcc ccgccgggtc gctgaagatg
     6481 atcgtcggca ccgacctcat catcctcggc atcctgcttc tcgtcgccta cgttctaggc
     6541 cgtgccgggc ggttggtggg gctgcccgcg ataccgatct acatggccgt cggcctcttg
     6601 tgcagcccgc acacgacatg gttcccgttg aatttcgacc ccgggcacat cgagttgatc
     6661 gcgatagtcg ggctcgttct gctgctcttc agcctgggcc tcgagttcga ccaagacgag
     6721 ttcttcggca acgccggcaa gcttctggtg accggcggct tctacatcgt tctgaacatc
     6781 ggcgtcgggt tcgtgttcgg catgctggtc ggctgggggc cccgggaggc gctcgtcatc
     6841 gccggcatgg tgggcacgtc gtcgagcgcg atcatcacga agcttctcat cgaactgcgg
     6901 cgcctggcca acgacgagac ccccatgatc ctcggcgtga cggtcgtcgg cgacatcttc
     6961 atcgccgtct acctcgcgat cgtctccgtc gtcttgagcg gcaagacgga ggtctggccg
     7021 gtcgtcgtgc agctgacgac ggcgttctgc ttccttgtcg ccatgttcgg cgtcgctcgg
     7081 ttcggcggca aggtcgtctc ccggctgctg cgcaccaaag acgaggagct cttcaccatc
     7141 ctcttcttcg gcctcgcact gcttttcgcc ggcatcggcg aggtgatcgg cgtcagcgac
     7201 gcgatcggcg cgttcttgat cggcctcgtc ctcggtgcga cgactttcca cgcacgcctg
     7261 gaacgggtga ctgttccgtt gcgcgatatc ttcgcagcgt tcttcttcgt gaacttcggc
     7321 ctcgggttga acgtcaccga gttcccggcc gtcgtgggca tcgtgctctt ggccgtcttg
     7381 atgacgttcg tcttgaatat cgtctcgggc gtcgcgatcg cgaagatgta cgggttccac
     7441 ctgccggagg gcgtcaacgc ggccgcgatc ctggtgaacc ggggcgagtt cacgttgatt
     7501 ctcgccacct tgtcggtagc ggccggcttg aacggcctcc tctccgcgtt cgccgggctg
     7561 tatgtgctcg tgatggcggt tctcgggccg ctgttcgcag ccaattccga acgcctcggt
     7621 gctctactga gacggacgag ggcggcgccg cgcgccgcgg tgccgaaccc ggtgcgcgac
     7681 gaggagatcg ccttggtcga tgccgtcacc gacgacgacc cggagcgcac ccaacgcgcc
     7741 atcgaccgca tggtcgagca ggcgatgatg ggtgagggcg tggaaggcga agagggagac
     7801 gaccattagc gacgtgcgac catcggccgg cccagggcgt gacgtgggcg gcgtcaccga
     7861 ggtaaccctt ttccgcacga tgctgcgacc ccggtggatc gtcgccttga tccttgcgat
     7921 ggcggtcgcc gcgggtttcg cctacctcgg caagtggcag ctgggccggg ccatccagtc
     7981 ggtcgagccg gccttcgcgc cgacggaaca ggtgaagccg ttcgtcggcg aggcgcggcc
     8041 cggcaaagac gtgacggacg ccagcatcgg gcagcgggtc agcctgcagg gcagcttcgt
     8101 cccgggcgac taccagctgg tcgccgaccg gctgaaccac ggccgcaccg gctactgggt
     8161 ggtcgggcat ttgaccctcg aggagacgag cgcctcaggc caagcggagg ccgtcgcggt
     8221 ggcacgcggc tgggcgccga ccaagagcaa ggcgcaggcc gccgcccggg aactctcctc
     8281 caggccggct tccccggtgg agctgaccgg gcggatgctg ccgactgagg cacccgtcgt
     8341 gccgcccgcg ggcagcgcgc cgaagacgat gacgatgctc gccgtcgccg acctcgtgaa
     8401 caggtggcac ggcatggacg acgcggtcgt cttcaccgcc tacgtcgtgg agcacggcgc
     8461 ggtcgcgggg ctcgacgaga tctattcgcc tccgccgatc gtgcagcact ccctcgatct
     8521 tctgaacgtc ttctacgcga tcgaatgggc tgtttttgcc ggcttcgcgc tgtacctgtg
     8581 gtaccgggtc gtaaaggatt actgggagcg ggagaacggc gacgagaccg gcgtcgtgcg
     8641 ggtcggcctc gcgtcccccg gccggcccgg cgacgggcag aaagtagact gaagtcatgg
     8701 catacacacc cggctccgac gaagatgcgt cgctcctggc gcgcattctc gcgcagctgc
     8761 gttccgccga ttcggccagg ccccgccgca tcgacctgcc gaagattccc ggtgcgctgc
     8821 gtctttaccg agtcaccgcg tacgtcacgg ggaccatgct gctgctgctc gtcgccgaga
     8881 tgatcgtgaa atatggcgtc ggctattcgc tgtacttcgg cgtgccgggc cggcccgggt
     8941 tcagcttcgt cgaattcaac ccgctgcaga cgaacctgca cggcgtcgac gtctcgacgg
     9001 cgatcctcat cgtgcacggc tggctgtacg tcgtctacct cttcgccgat ttccggctgt
     9061 ggagcctcat gcggctctcg ttcacgaagt tcatccagat cgcgttgggc ggcgtcgtgc
     9121 cgttcatgtc attcattgtc gagtacttca tcacccggca ggtgcggcgc ttcctcgcct
     9181 ccgtccggca ggaggacgag gctcgggcga gcgccgtcga tgtgcacgcc gcgggggagg
     9241 agacccatta gcggggcgag cgccgcggcg gcctcgtcgg cgacggtcct ggtcgtcgac
     9301 ttcggagccc agtacgcgca gctcatcgcc aggcgggtgc gcgaggcaca cgcgtattcc
     9361 gagatcgtgc cgcataccgt caccgccgcc gagatccaga ggcggcaccc ggccggcatc
     9421 atcctgtcgg gcggcccggc gagcgtctac gcgccggacg cccccggctt cgactccggc
     9481 atcttcgatc tcggcgtgcc ggtgctcggc atctgttacg gctttcagct gatggcgagc
     9541 gccttgggcg gcacggtcga gcacaccggg cggcgggagt atgggtcgac cgacgtcacc
     9601 gtcaacgagg ccggatgcca gggggtgctc ctcgccggcc agccccaggc gcagcgggcc
     9661 tggatgagcc acggcgattc cgtgaccggc gcgccaaaag acttcgccgt gctcgcctcg
     9721 tcgcccgcga caccggtcgc cgcgttcgct aaccccaggc gagggcttta cggggtgcag
     9781 tggcaccccg aggtgcggca ttccgaccag ggccaacgcg tgctggagaa cttcgttcac
     9841 gaggtggccg gcgtcggctc cgagtggacg agcggcaacg tcatcgacga ccaggtcgcg
     9901 gcgatccggg ctcaggtggg cacggggcgg gtgatctgcg gtctttccgg cggggtggac
     9961 tcggcggtcg ccgcagcgct cgtgcaccgg gcggtcggcg accagctgac ctgcgtcttc
    10021 gtcgatcacg gcctgctacg ccgcgacgaa cgcattcagg tggaacgaga ctacgtcgcg
    10081 tccacggggg tgcggctcgt caccatcgac gcggccgacc ggttcctcgc cgcgctggag
    10141 ggcgttaccg acccggaaga caagcgcaag atcatcggcc gcgagttcat tcgcaccttc
    10201 gaagcggccc aggcacgcct cgtcgccgag gcgcacgacg ccggcgaccc cgtccggttc
    10261 ctcgtgcagg gcactctcta tccagacgtc gtcgaatccg gcggcggctc cggcaccgcc
    10321 aacatcaaga gccaccacaa tgtcggcggg ctgcccgacg acctggcatt cgagctcgtc
    10381 gagccgctgc gcaccctgtt caaagacgag gtgcgtgcga tcgggcgcgc cctcgggctg
    10441 cccgaggtga tcgtcagtcg gcagcctttc cccgggccgg ggctcgggat ccgcatcgtc
    10501 ggcgcggtca ccgccggccg gctggcgact ttgcgcgccg ccgacgcgat cgcccgtgaa
    10561 gagctgaccg gcgccgggct cgacggcgag atctggcagt gcccggtcgt gctcttggcc
    10621 gacgtgcatt ccgtcggggt gcagggcgac gcccgcacct atgggcatcc cgtcgtcttg
    10681 cggccggtga cgagcgaaga cgcgatgacc gccgactggt ccaggctgcc ctacgacgtg
    10741 ctttcccgta tctcgaaccg catcacgaac gaggtggagg gcatcaaccg ggtcgtgctc
    10801 gacgtgacct cgaagccgcc cggcaccatc gagtgggagt gacgcgagcg cggcgaggag
    10861 gggaatggac gaggttgtcg cgcggctgct ggccgcgcta ccggacgatc gggtgcggca
    10921 gaacgcggaa cctcgtggcc ggtcagccga gatctcgccc tcggagcgta gcggctccgc
    10981 ctgcctggta gacacgacac gagccgcgat cgcggccaga tcttcggacc gctccggcct
    11041 tgcgggtgag gcgggtgccc gcgtcgttgt gcgcccgagg acgacggcgg aggtggcccg
    11101 ggtcatgcgg atcgccgacg agacgcgcac ccccgtggtg ccgcaaggca ggctgacggg
    11161 gctcgccggg ggcgccaacg cgatcgccgg cgcgatcctc ctcgacgtga gcggtatgga
    11221 ccgcatcctc cgcgtcgacc gggaagagct cgcggcggtc gtgcagcccg gcgtgatcgt
    11281 cgccgatctg cagaaggccg caagggaaca cggtcttttc tatgcgcccg acccggcctc
    11341 cgcgcggatc gcctcgatcg gcggtaccgt ggcgacgaac gccggcggca tgcgcgccgt
    11401 caagtacggg gtgacccgcg actgggtccg ctccctcgaa gtcgtgaccg cagacggcag
    11461 cgtcgtgcgc acccgaccgc agacggtcaa ggcggtggcg ggttacgacc tgacggggct
    11521 cgtcgtcggt tcggagggca ccctcgcggt cgtcaccgag gccacggtgc ggctgatccc
    11581 cgcgccggcc gcgccgacgg gcatcgccgc gacgtttcgc gacgtgcccg cggcgctcgg
    11641 ggccgtcgcc ggcatcatca cgggcggtgc gctgccggca acgatcgagc tgctcgacga
    11701 ggcgtcgatc cgcgcgatcc gccgttatgc ggccgatgga gacgacccgg aggccaggtt
    11761 cgcggccgag gcgctgcccg agggagcggg cgcctggatc gtcgtcgcca ccgacacccc
    11821 gaccgcggcg gccgatcttc ggcgttacga gcacgcctgc cgagagaacg gcgctcagac
//
LOCUS       DASTNL010000232         7057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1651693, whole genome shotgun sequence.
ACCESSION   DASTNL010000232 DASTNL010000000
VERSION     DASTNL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651693"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1085
                     /locus_tag="VFQ96_07465"
     CDS             69..1085
                     /locus_tag="VFQ96_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0257662.1"
                     /translation="MVRLLGPQDRFLTTIEREHPDVRLHVRGNEIVLVGPTPAVTETR
                     QLIRELQQMIQSGQSLDTIEVASSARLIHDHAGRPSEVLGKTIVQARGHAVRPKTLGQ
                     KRYVDAIDHNTIVFGIGPAGTGKTYLAMAKAVEALQHRAVERIILTRPAVEAGERLGF
                     LPGTLTDKIDPYLRPLYDALNEMMDPDQVPKLLASGTVEVAPLAYMRGRTLNDSFIIL
                     DEAQNTTPEQMKMFLTRLGFGSTMVVTGDITQVDVPAVASGLRVVGSVLTGIDGIAFS
                     YLTSEDVVRHSLVSRIVDAYTRYEAATQAARGRGRGGGRGATRPAGGRGSRIAGRRGA
                     QRGR"
     gene            1089..1550
                     /gene="ybeY"
                     /locus_tag="VFQ96_07470"
     CDS             1089..1550
                     /gene="ybeY"
                     /locus_tag="VFQ96_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU0257663.1"
                     /translation="MSTEVNNESDIDITEETLQRLARYALDALHVSPDADLGILLVDE
                     AAMEQLHVQWMDEPGPTDVLSFPMDELRPGTGDDPTPAGLLGDIVLCPQVAQLQAERA
                     QHSLMDELQLLTTHGLLHLLGFDHADPEGEREMFGLQRDILVGFTAAERRR"
     gene            1558..2937
                     /locus_tag="VFQ96_07475"
     CDS             1558..2937
                     /locus_tag="VFQ96_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0257664.1"
                     /translation="MSLTFLIIAAALIAAGGLMAATDAAITSLSNADIDDLADASHVA
                     SSLRAIARDPGPHLNAVAFVRVSVEMTSAVLATLAFTLLFEPVWLVLVVAALVMTGAS
                     FVLAGASPRSVGRMHARPLLRLTAPLVHAIRVLLGPVADALVAFGNRVTPSRARGAGV
                     ASEEQLLSIVDEAAELDVLEEDDREFIHSLFDFNETLVREVMVPRTDMVVVRAGTGER
                     AALSAFLTHGVSRVPVVRDDIDDVVGVLYLRDLAGRLFAGTRHPAQGAPGGDAAERSG
                     LPGAGEGTDRPAAGEERAAGIEALARPAVFVPESQKADALLAQMQADAVHMALVVDEY
                     GGIAGLVTLEDLIEELVGDIADEYDREEELVQPLGPGRYRVSARLPARDLADLFDISL
                     EEDDVDSVGGLVGKLLGRLPVVGSRVEAFGLVFTAERAEKHRRRLSTVIVERRAGSDA
                     PGTAEGENG"
     gene            2934..3827
                     /gene="era"
                     /locus_tag="VFQ96_07480"
     CDS             2934..3827
                     /gene="era"
                     /locus_tag="VFQ96_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0257665.1"
                     /translation="MSDYRAGFVSFVGRPNVGKSTLMNALVGEKVAITSPKPQTTRRA
                     IRGIMHRKNGQLVIVDTPGVHRPRTLLGERLNSLVEETLSGVDVIGACFPADERLGPG
                     DRFVNTRLDAFPRAKKVAIVTKIDAASRPQTSEKLLAVARLREWEAIVPISAANHIQL
                     DTLATELIRLLPQADHPLYPPDAVTDESLEDRVAELVREAALDGVRDELPHSLAVTVE
                     DIVTRDDKDLVEVYANIFVERDSQKGIVIGRSGVRLRQVGAEARPAIEELLGSHVFLA
                     LHVKVAKDWQRDPKQLRRLGF"
     gene            3835..4680
                     /locus_tag="VFQ96_07485"
     CDS             3835..4680
                     /locus_tag="VFQ96_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0257666.1"
                     /translation="MPAAVPSSPAKAPKPRFFLGRDGVRLAYREVGEGRPLLLVHGYV
                     SNAWVNWIRFGHAARLAAAGFRVIMPDLRAHGDSARPKSPGAYPPDILADDAFSLVAR
                     LGLDDYDLGGYSLGGRTVVRMLARGATPGRAIVAGMGWEGIVDPAFRDRFFERLFAGL
                     GGHRPGGAEWRAWHFVRSMHGDPEALRMILQTSVPTGPAELRAIAVPCLVLLGDRDDE
                     HGSGERLAGALGQGTFASAPGTHMSVVTKPEFGRQMSEFLTRGRPTSAAGRSGKEDTP
                     GGANL"
     gene            4794..6557
                     /gene="leuA"
                     /locus_tag="VFQ96_07490"
                     /pseudo
     CDS             4794..6557
                     /gene="leuA"
                     /locus_tag="VFQ96_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            6622..>7057
                     /gene="recO"
                     /locus_tag="VFQ96_07495"
     CDS             6622..>7057
                     /gene="recO"
                     /locus_tag="VFQ96_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0257667.1"
                     /translation="MPKLYRDEGVVLRTHKLGEADRIVVLLTRENGKVRAVAKGVRRT
                     SSKFGSRLEPFSHIDVQCHVGRSLDIVTQVETLTPFGANIAADYPRYTAGTAMLETAE
                     RLTPEEREPSVQQYLLLVGGLRALVAGDHDSGLVLDSYLVRSL"
BASE COUNT         1131 a         2425 c         2413 g         1088 t
ORIGIN      
        1 ccgtggctga cgaggacggc gagcgccccg gttattcgac ggtggcgttg cgcatcgacg
       61 gcgtcgagat ggtgcggctg ttggggccgc aagaccgctt cttgacgacg atcgagcggg
      121 agcaccccga cgtgcggctg catgtgcgcg gcaacgagat cgtgctggtc ggcccgacgc
      181 cggcggtcac cgagacccgg cagctgatcc gcgagctgca gcagatgatc cagtcgggtc
      241 agagtctcga cacgatcgag gtggcgtcct ccgcgcgtct catccacgat cacgcgggtc
      301 ggccgtcgga ggtgctgggc aagacgatcg tgcaagcccg cggccatgcg gtgcggccga
      361 agacgctcgg ccagaagcgt tacgtcgacg cgatcgacca caacacgatc gtgttcggca
      421 tcggcccggc gggaacgggc aagacgtatc tcgccatggc gaaggcggtc gaggcactgc
      481 agcaccgcgc ggtcgaacgg atcatcctca cccggcccgc cgtcgaggcg ggggagcgcc
      541 tcggcttctt gcccggcacc ttgaccgaca agatcgaccc gtacctgcgc ccgctctacg
      601 acgccttgaa cgagatgatg gatccggatc aggtgccgaa gctcctcgcc tccggcaccg
      661 tcgaggtcgc gcctctggcg tacatgcgcg gccgcacgtt gaacgactcg ttcatcatcc
      721 tcgacgaggc gcagaacacc acgccggagc agatgaagat gttcctcacc cggctcggct
      781 tcggctcgac gatggtcgtc accggcgaca tcacgcaggt ggacgtgccg gctgtggcga
      841 gcggcttacg ggtggtcggc tccgtcttga cgggcatcga cggcattgcc ttcagctatc
      901 tgacgagcga ggatgtcgtg cgtcattcgc tcgtcagccg catcgtcgac gcctacacgc
      961 ggtacgaggc cgcgacgcag gccgcccggg ggcgcggccg tggcggcggg cggggtgcca
     1021 ctcggcctgc gggcggccgg ggcagccgca tcgcgggccg gcgcggcgcc cagaggggca
     1081 ggtgagcggt gtcgaccgag gtgaacaacg agtcggacat cgacatcaca gaagagacct
     1141 tgcaacgcct cgcgcggtac gcgctcgacg cgctgcatgt gagccccgac gcagacctcg
     1201 gcatccttct cgtcgacgag gccgcgatgg agcagttgca cgtgcaatgg atggacgagc
     1261 ccgggccgac cgacgtcttg agctttccga tggacgagct gcgccccgga acgggggacg
     1321 acccgacgcc cgcgggtctt ctcggcgaca tcgtgctgtg cccccaggtc gcacaactgc
     1381 aggccgagcg cgcccagcat tccctcatgg acgagctgca gctcttgacc acccacggcc
     1441 tcttgcatct gctcggcttc gatcacgccg acccggaggg ggagcgcgag atgttcgggc
     1501 tgcagcgtga catcctcgtc gggttcacgg cggccgaacg acgacgctga gcactccgtg
     1561 tcgctcacct tcctcatcat cgcggccgcc ttgatcgcgg ccggcgggct gatggccgcg
     1621 acggacgcgg cgatcacgtc gttgtcgaac gccgatatcg acgatctcgc cgacgcctcg
     1681 cacgtcgcgt cgtcgctgcg cgcgatcgcc cgagacccgg gcccgcacct gaacgccgtc
     1741 gcgttcgttc gggtcagcgt cgagatgacc tcggcggtgc tggccaccct cgccttcacg
     1801 ctgctcttcg aacccgtgtg gttggtgctc gtcgtcgcgg cgctcgtcat gacgggtgcc
     1861 tcgttcgttc tggcgggtgc gagcccacgc agcgtggggc gcatgcatgc caggcccctg
     1921 ttgcgcctga ccgccccgct cgtgcacgcc atccgggttc tcctcggccc ggtcgccgac
     1981 gccctcgtcg ccttcggcaa ccgggtcacc ccgtccaggg cccgcggtgc cggggtcgcg
     2041 agcgaagaac agctgctcag catcgtcgac gaggcggcgg agctcgacgt gctcgaagaa
     2101 gacgatcggg agttcatcca ttcgcttttc gacttcaacg agaccctcgt gcgtgaggtc
     2161 atggtgccgc gcactgacat ggtcgtcgtg cgggccggca cgggcgaacg tgcggcgctg
     2221 tcggccttcc tcacgcacgg cgtctcccgg gtcccggtgg tgcgcgacga catcgacgac
     2281 gtcgtggggg tcctctacct gcgcgatctc gccggccgtc tcttcgccgg cacccggcac
     2341 ccggcccagg gggccccggg cggcgacgcc gccgagagga gcggcctgcc cggcgcgggc
     2401 gaggggaccg acaggccggc ggccggcgag gagcgtgcgg ccggcatcga agcgctggcg
     2461 cggccggcgg tcttcgtgcc ggagtcgcag aaggccgacg cgcttctggc ccagatgcag
     2521 gccgacgccg tgcacatggc actcgtcgtc gacgaatacg gcggcatcgc ggggctcgtg
     2581 accctcgaag acctgatcga agaactggtc ggcgacatcg ccgacgaata cgatcgtgag
     2641 gaagagctcg tgcagccgct tggcccgggc cgttatcggg tgagtgcgcg gctgccggcc
     2701 cgtgacctcg ccgacctgtt cgacatctca ctggaggaag acgacgtcga ctcggtcggt
     2761 gggctggtcg gcaagcttct cggcaggctc cccgtggtcg gctcccgggt cgaggcgttc
     2821 ggcctcgtgt tcaccgccga gcgggcggag aagcatcgcc ggcggttgag tacggtgatc
     2881 gtggagcgcc gtgcgggctc agacgcgccg ggtacggcag aaggggagaa cggatgagcg
     2941 actatcgcgc agggttcgtg tcgttcgtgg gccggcccaa cgtcggcaag tcgacgctca
     3001 tgaacgccct cgtcggcgag aaggtcgcca tcacgagccc gaagccgcag acgacgaggc
     3061 gtgccatccg gggcatcatg caccgcaaga acggccagct cgtcatcgtc gacacgccgg
     3121 gcgtgcaccg cccccgcacg ctcctgggcg agcggctgaa ctccctggtc gaagagaccc
     3181 tctccggcgt cgacgtcatc ggtgcctgct tcccggccga cgaacgtctc ggccccggcg
     3241 accggttcgt gaacacccgc ctcgacgcgt tcccccgggc gaagaaggtc gcgatcgtca
     3301 cgaagatcga cgcggccagc cggccacaga cgagcgagaa gctgctggcc gttgcccggc
     3361 tgcgggagtg ggaggcgatc gtgccgatct cggcggcgaa ccacatccag ctcgacacgc
     3421 tcgcaaccga gctgatccgc ctgctgccgc aggccgatca tccgctttac ccgcccgacg
     3481 cggtgaccga cgagtcgctg gaagaccggg tcgccgaact cgttcgcgag gctgcgctcg
     3541 acggggtgcg cgacgagctg ccgcattccc tcgccgtcac cgtcgaggac atcgtcacaa
     3601 gagacgacaa agacctcgtc gaagtgtatg cgaacatctt cgtcgagcgc gacagtcaga
     3661 agggcatcgt catcggccgt tccggcgtgc gcctgcgcca ggtcggcgcc gaggcgcggc
     3721 cggcgatcga agagctgctc ggctcgcacg tcttcctcgc cctgcacgtg aaagtcgcca
     3781 aagactggca gcgcgacccg aagcagcttc gccgcctggg gttctaggcc tgccatgcct
     3841 gccgccgtgc cctcgtcgcc cgcaaaggct ccgaagcctc ggtttttcct cggccgcgac
     3901 ggcgttcgcc tcgcctaccg ggaggtgggg gagggccggc ccctgttgct cgtgcacggt
     3961 tacgtttcga acgcctgggt gaattggatc cgattcggcc acgccgcccg cctggccgcg
     4021 gcgggctttc gcgtcatcat gccggatctg cgcgcgcacg gcgacagtgc ccggccgaag
     4081 agccccggcg cctatccgcc cgacatcctc gccgacgacg cgttcagcct cgtggcccgg
     4141 ctcggcctcg acgactatga cctcggcggc tattcgctgg gcggccgtac cgtcgtgcgc
     4201 atgctggccc gcggcgccac cccgggtcgc gcgatcgtcg ccggcatggg gtgggagggg
     4261 atcgtcgacc cggccttccg cgacaggttc ttcgagcggc tcttcgccgg cctcgggggc
     4321 catcggccgg gcggggccga gtggcgtgcc tggcacttcg tgcgcagcat gcacggcgac
     4381 cccgaggcgt tgcgtatgat cctccaaacc tccgtgccga ccggcccggc cgagcttcgg
     4441 gcgatcgccg tgccctgctt ggtgctcctc ggcgaccgcg acgacgagca cggctcgggt
     4501 gagaggctcg ccggagcctt ggggcagggc acgtttgcct ccgcgcccgg aactcacatg
     4561 agcgtcgtga caaagccgga gttcgggcgg cagatgtcgg agttcctcac acggggccgg
     4621 cccacctcgg cggcagggcg gagcggaaag gaagacacgc ccggtggtgc taacctttaa
     4681 cccgtgttcg gcatgctcct ccttagctgc cgcgacgagt cttagatcgc tttggccatc
     4741 ctcgtcgcgg agtctgtcgt tggcttgaac ctgcgagacg aggagaagaa gaagtgcgga
     4801 acacccagaa acccagcggg atgccgattc acaagtatcg gccgttccac gagcaggttc
     4861 gcgtagagct gcccgaccgc acctggccca cgaagcgcat cgaacacgcg ccgcgctggt
     4921 gcgcggtcga ccttcgagac ggcaaccagg cgctcatcga cccgatgggt gcggaacgca
     4981 aacgcgccat gttcgacctt ctggtctcgc tcggctacaa agagatcgag gtgggctttc
     5041 cgagcgccag ccagaccgat ttcgacttcg tccgtcagct gatcgacgag cacctgatac
     5101 ccgacgacgt gaccatccag gtgttgaccc agtcgcgcga gcacctgatc aggcgcacct
     5161 acgaggcgat ccgaggcgcc aagcaggcca tcgtgcacct ctacaactcg acgagcgtgc
     5221 tgcagcgcga ggtcgtcttc cgcaaagacc ggcaggggat catcgacctc gcgctctccg
     5281 gagcgaggct ctgcaagagc atggagccga gcgttccgta aacgaccgtg tactacgagt
     5341 actcgcccga gagctacacc ggcacggagc tggaattcgc ggccgagatc tgcaaccggg
     5401 tgctcgaggt cttcacgccg acgccggagc gccagacgat catcaacttg ccggccaccg
     5461 tcgagatggc cacgccgaac gtctacgccg actccatcga atggatgtcg agacatctcg
     5521 agcatcggga gaacgtcatc ctctccctgc acccccataa cgaccgcggc acagcgatcg
     5581 ccgccgccga gctcgccctg atggccggcg ccgaccggat cgaagggtgc ctcttcggca
     5641 acggggagcg caccggcaac gtagacatcg tgacgctcgg catgaacctc ttcacgcagg
     5701 gcatcgaccc gcagatcgat ttctccaaca tcgacgagat caagcgcacg gtcgagtatt
     5761 gcaaccagct gcccgtccac gagcgcctgc cgtgggccgg cgacctcgtc tacacggcct
     5821 tcagcggctc ccatcaagac gccatcaaga aaggcttcga ggcgatgcag gccgaggcgt
     5881 ctcgccgcgg gtgcagcgtc gacgacctcg tctgggcggt gccctacctg ccggtcgacc
     5941 cgaaagacgt cggccgcacc tacgaggccg tcattcgcgt caactcgcag tccggcaagg
     6001 gcggggtcgc ctatctcttg aaggccgatc acgcgctcga gctgccgcgc aagctgcaga
     6061 tcgagttctc tgccgtcgtg caggcgaaga ccgacgcgag cggcggggag gtcacgagcg
     6121 acgagatctg ggcgatcttc caagatgagt acctgcccgt ctcggccgag aacgccaagt
     6181 gggggcgctt cgaactcgcc cgcatgcgta ccgagaacga gatggatggg cggctgaacc
     6241 tgcacatcga cctgcgcgac ggcgagcaca cgacgagcgt cgaggctgcg gggaacggcc
     6301 cgatggatgc cttcctcacc gccctgcggg ctaacggcat cgaggtgacg ctgtacgact
     6361 acgtggagca cgccctctcc ggcggcggcg acgcgacggc cgccgcctat gtcgacctcg
     6421 acgtgaacgg gcgcaggctg tggggtgtcg gcatcgacgc cgacatcgcg accgcgtcgt
     6481 tgaaggcgat catctccgcg gtcaaccgcg ccgtgcggca ggcggaggcg agcgagcccg
     6541 tcgcggtcgt cgcctgagcg ctcggccgta cccacgcggc ggtgccgggc gacggccggt
     6601 ggaaggcgcg ataatcgagg gatgccgaag ctctaccgag acgagggtgt ggtgttgcgc
     6661 acccacaagc tcggcgaggc cgaccgcatc gtcgtgctcc tgacgcggga gaacggcaag
     6721 gtgcgtgccg tcgcgaaggg ggtgcgacgc acctcgtcga agttcggctc gcggctggag
     6781 ccgttcagcc acatcgacgt gcagtgccac gtcggccggt cgctcgacat cgtcacgcag
     6841 gtcgagacgc tgacgccgtt cggcgcgaac atcgctgcgg actatccccg ctacaccgcc
     6901 ggcacggcga tgctcgagac cgccgagcgg ctgacccccg aggagcgcga gccctcggtc
     6961 cagcagtacc tcctcctcgt gggcgggctg cgcgcgctcg tcgcgggaga ccacgactcc
     7021 gggctcgtcc tcgactccta cctcgtgcgc tccctgt
//
LOCUS       DASTNL010000233         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_359048, whole genome shotgun sequence.
ACCESSION   DASTNL010000233 DASTNL010000000
VERSION     DASTNL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359048"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..>2142)
                     /gene="infB"
                     /locus_tag="VFQ96_07500"
     CDS             complement(65..>2142)
                     /gene="infB"
                     /locus_tag="VFQ96_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU0257668.1"
                     /translation="YAGAGGRPGQRAGGPGRPGSARGGAAARPGATGRPGGGGRSFGR
                     GRGRTAGAFGRGGGKSRARKSRRTKRQEFEMREAPLVGGVKVPHGDGNTVVRLRRGAS
                     VSDFADKIDVSPGDLVTVLFHLGEMATATESLDEATFEVLGAELGYRIQIVSPEDEDK
                     ELLEGFDIDLAGELEAESDEDLLPRPPVVTVMGHVDHGKTRLLDAIRHANVVAGEAGG
                     ITQHIGAYQVLTEHDGTERAITFIDTPGHEAFTAMRARGAQVTDIAILVVAADDGVMP
                     QTVEALNHAQAANVPIVVAVNKIDKPDANPAKVRQQLTEFGLVAEEYGGDVMFCDVSA
                     RERTGIAELLDAVLLTADAGLDLRANPHKAARGVAIEAKLDKGRGSVATVLIQSGTLR
                     VGDSIVAGTAYGRVRALSDENGDAVDEALPARPVQVQGLSSVPRAGDTFIVTDDDRTA
                     RQIAEKREAAERNAQLAKARKRISLEDFTKALQEGKVQSLNLIIKGDVSGAVEALEDS
                     LLDIEVDDSVQLRILHRGVGAITESDIDLATIDDAIVIGFNVRPDVKARERAAREGVD
                     VRFYSVIYNAIEDIENSLKGMLKPEFEEIQSGVAEVREVFHSSKFGNIAGVYVRTGTI
                     TRNAKARVIRDGVVVGDGLAIESLRRFKDDVTEVKTDFECGIGLGRFNDIQVGDEIET
                     IELKEKVRA"
BASE COUNT          298 a          752 c          719 g          373 t
ORIGIN      
        1 tcggagatgc cggcggggcc gcggcgacac acacgtgccc gcgcggcccg ccggcatcgc
       61 accgtcaggc gcgcaccttc tccttgagct cgatggtctc gatctcgtca ccgacctgaa
      121 tatcgttgaa ccggccgagc ccgatgccgc attcgaagtc ggtcttgacc tcggtgacat
      181 cgtctttgaa ccggcgcagc gactcgatcg cgagcccgtc gccgacgacc acaccatcgc
      241 gaatgacgcg ggccttcgcg ttgcgggtga tcgttccggt gcgcacgtag acgcccgcga
      301 tgttgccgaa cttggaggag tggaacacct cgcgcacctc ggcgacaccc gactggatct
      361 cttcgaactc gggcttgagc atgcccttga gcgagttctc gatgtcttcg atcgcgttgt
      421 agatgaccga gtagaagcgc acgtcgacgc cctcacgggc cgcgcgctcc cgggccttga
      481 cgtcggggcg cacgttgaag ccgatgacga tcgcgtcgtc gatcgtggcg agatcgatgt
      541 cactttcggt gatcgcaccg actccgcgat gcaagatgcg caactgcacc gagtcgtcca
      601 cctcgatgtc gagcagcgag tcttccagcg cctccacggc gccggagacg tcgcccttga
      661 tgatgaggtt gagggactgc accttgccct cttgcagcgc ctttgtgaag tcttcgaggc
      721 tgatgcgctt acgcgccttc gccagctggg cgttccgctc cgctgcctcg cgcttctcgg
      781 cgatctgacg ggcggtgcgg tcgtcgtccg tgacgatgaa ggtgtcaccg gcgcgcggca
      841 cgctggagag tccctgcacc tgaacgggcc gggccggcaa cgcctcgtcg acggcgtcgc
      901 cgttttcgtc gctcagcgca cgcacacggc cgtaggcggt gcccgcgacg atcgagtcgc
      961 cgacccggag ggtgcccgac tggatgagca cggtcgccac cgagcctcgg cccttgtcga
     1021 gcttcgcctc gatcgcgacc ccgcgggccg ccttgtgcgg gttcgcccgc aggtcgagac
     1081 cggcatcggc ggtgaggagc acggcgtcaa gcagctcggc gatgcccgta cgctcccgcg
     1141 ccgagacgtc acagaacatg acgtctccgc catactcctc ggcgaccagc ccgaactcgg
     1201 tgagctgctg acgcaccttg gcggggttcg catccggctt gtcgatcttg ttcacggcga
     1261 cgacgatcgg cacgttcgcg gcctgcgcgt ggttcaaggc ctcgaccgtc tgcggcatga
     1321 cgccgtcgtc ggcggcgacc acgaggatcg caatgtcggt gacctgtgcg ccgcgcgcac
     1381 gcatggcggt gaatgcctcg tggcccggcg tgtcgatgaa ggtgatcgcg cgctcggtgc
     1441 cgtcgtgctc ggtgagcacc tggtaggcgc cgatgtgctg ggttatgccg cccgcctcgc
     1501 cggccacgac gttggcgtgc cggatcgcgt cgagcaggcg agtcttgccg tggtcgacgt
     1561 ggcccatgac cgtgacgacc gggggcctcg gcaggaggtc ctcgtcgctt tcggcctcga
     1621 gctcgcccgc cagatcgatg tcgaagccct caaggagctc cttgtcttcg tcttcgggcg
     1681 agacgatctg gatgcggtag ccgagctcgg cgccgagaac ctcgaaggtc gcctcgtcga
     1741 gcgactccgt ggcggtcgcc atctcaccca ggtggaagag gaccgtgacg aggtcgcccg
     1801 ggctgacgtc gatcttgtcg gcgaagtcgc tgaccgaggc gccgcggcgc agacggacga
     1861 cggtgttgcc gtcgccgtgc ggaaccttga cgccgccgac gagcggcgcc tcccgcattt
     1921 cgaattcttg cctcttggtg cgcctcgact tgcgggcgcg gctcttgccg ccaccgcggc
     1981 cgaacgcgcc cgcggtgcga ccgcgaccac ggccgaaact gcggccgcca ccgcccggcc
     2041 tacctgtcgc gccgggcctg gccgctgcac cgccccgagc acttccgggc ctgcctggcc
     2101 ctccggcccg ctgccccggc cggccgccgg cacctgcata cg
//
LOCUS       DASTNL010000234        12507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_917100, whole genome shotgun sequence.
ACCESSION   DASTNL010000234 DASTNL010000000
VERSION     DASTNL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917100"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..780
                     /locus_tag="VFQ96_07505"
     CDS             214..780
                     /locus_tag="VFQ96_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257669.1"
                     /translation="MAWPVGCWVFVLTAIAVVQVVRAQWFDAAVFFLASAGVSLDAVF
                     RPRFHPRRSLPPGVLAAAALVAGLVIAALPRHGALMRATVIIAGIAAVAVGWAGWAIR
                     APQAGVAFSPGVRALALTWAAIMVAGCLWELFQFLMSVAEPSGAWFSLSDLLNPFVGQ
                     WVGKAAFTAAWLVCGVWLLRRGGRRAER"
     gene            777..977
                     /locus_tag="VFQ96_07510"
     CDS             777..977
                     /locus_tag="VFQ96_07510"
                     /inference="COORDINATES: protein motif:HMM:NF040017.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HEU0257670.1"
                     /translation="MKILSWTMFLLAGAALVVGRARVRAHHADATGRALFDRAMADRR
                     IRVALIVIWWWLGWHFLAGQTL"
     gene            1066..1470
                     /locus_tag="VFQ96_07515"
     CDS             1066..1470
                     /locus_tag="VFQ96_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEU0257671.1"
                     /translation="MRRWIWRKYFIAVAAGLAAALSSTLVNRGADITMLVMAALGLLI
                     ALFGFIGVFAILEPRLGPSGLPVALFGVLLFLAPWVMGYVPHAGQAWTSWVCGLVGLL
                     VGLWGIAGTRGKPLPPRTQPRRDRGSRRPAAA"
     gene            complement(1503..3428)
                     /locus_tag="VFQ96_07520"
     CDS             complement(1503..3428)
                     /locus_tag="VFQ96_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0257672.1"
                     /translation="MPGRHATWVWASDVGLGRLRSASQAVLTMMVGMASEWFFIRLTH
                     ALQFPVPAGGPSAAAARVAFFDHATLLIGLMLGIMIGMMSGFTGPGVSGWRKALTDFG
                     WMPIPLTAGLALGSVLGDWHTATLIVLVFVFAAGTYARRAGPHGFQGGILGFIGYFLG
                     FFLREHAGIGPADLGWLAVEVVVAVAAGLLVQVTVFFPTAARMARRAVRSYVARSWRV
                     FAACDRILGAGGARPDASYTLMRRVDLAGEAALIVDFHLAGLHHDVPGLPAGDLGRRL
                     LLGHEQAVRRFASALCQGGAWPERDQLLTRLLRSGVRAAARGDLRRLDRVLATLQAYE
                     TRRAPAVESLGQVSAAAHAWRDAADRPRSLEQVSASPVVLFAGWLPGATLVSRAASVT
                     PGEGSATVLSPHVRAAVQMAVAGAAAIVAGSLLSGYRFYWAALGAFFILVGTNHAGEQ
                     VRKAVYRVAGTLVGAIIAVPVAHLAGSRPAPVIAVVLVSLFVGLYLMRVNYSLMIVGI
                     TVAVAELYVEFHEFTDGLLLLRLGETALGAAVTAAAVVLILPLRQGRVVTVAATRYQE
                     AVRAMSTGPANVDAARKADTAYQELMSVAAPRHAPYGPLVRTRNAGRRRLLRRLGDAH
                     RVRLPAPPSGGSTRPRQ"
     gene            3753..5081
                     /locus_tag="VFQ96_07525"
     CDS             3753..5081
                     /locus_tag="VFQ96_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257673.1"
                     /translation="MGTRRAWVVYSVGVFAYIISVTQRTTIGVAGVAATDRFRVDAAT
                     LSSLAVLQLVVYAAMQIPVGVLIDRYGPRRLMVTGTLLMLVGQVAVAFAPTIALAVAG
                     RVLVGGGDAMLFTSVMRLTASWFHGHIVAQLSQWIGNLGQVGQVLSAIPFAFLLHETG
                     WSVAFLSAAGCSLLAVVLVLAAIHDRPAGLPAITRPITLGRSVESLRAALARPGTQLG
                     FWSHFSSQSAGSIFSLMWGYPFMVYALGYRPQDAAALLIVPVGAAVFAGPVVGILSAR
                     FPLRRSNLVLGCVALMGVAWAAVLAWPGQPPFGVILFMLIAIGIGGPGSVIGFDYARM
                     FNPARSLGSANGVVNTGGFVASVAMIFLIGVILDAYNAAAGGGGPEHLYSMSAFRVAF
                     LAQFLVGGIGVTFLLRVRQRTRRGLAEDEGIAVGPLWEAVVRSWRRRKLR"
     gene            5281..5616
                     /locus_tag="VFQ96_07530"
     CDS             5281..5616
                     /locus_tag="VFQ96_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257674.1"
                     /translation="MVLQATLTIVVLMGGMIGIVVLGMLWQKFTALPRKPRGRTAKTR
                     NTKTPVRVREARTGEAQRNGSRGSETRESGTRTPAAPDQATPLGGASGPGERLPGGTG
                     DDTTGTDPS"
     gene            complement(5666..6076)
                     /locus_tag="VFQ96_07535"
     CDS             complement(5666..6076)
                     /locus_tag="VFQ96_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0257675.1"
                     /translation="MPSAPPLVVAASTVIRDGMGRILLVRRGHEPNKGRWSLPGGKAL
                     PGEDPRATACRETREETGLDIDVRRELWTIRVPDGDRRIFEIHTFLARNMVGEPRAAD
                     DAAELLWAGAREFATLPLTANLRELLDAVGLRAV"
     gene            complement(6139..6351)
                     /locus_tag="VFQ96_07540"
     CDS             complement(6139..6351)
                     /locus_tag="VFQ96_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257676.1"
                     /translation="MTREAKEVVRRMIWSGVAGVHAASAVMVAASVVAPNSNDAAAVP
                     WVVGGGVVTAAIAVLLVRHVLRRPRR"
     gene            complement(6420..6722)
                     /locus_tag="VFQ96_07545"
     CDS             complement(6420..6722)
                     /locus_tag="VFQ96_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEU0257677.1"
                     /translation="MKAVVDGVVLAEAPQEELIRIEGNWYFPPASLAADRFVPSDTPY
                     TCPWKGVCQYFTVQGSDGDRKDLAWSYPTPYPSAFERVGKDFSGYVAFDPKVDIVA"
     gene            complement(6808..7827)
                     /locus_tag="VFQ96_07550"
     CDS             complement(6808..7827)
                     /locus_tag="VFQ96_07550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0257678.1"
                     /translation="MKKIGFLSFGFWNEGAGSLVRTARDSLVQSVELAVAAQEIGIAG
                     AFYRVHHFASQQASPFVVMAAVAAKTTTLEMGTGVIDMRYETPLYLAEQAAQLDLLSE
                     GRLQLGISRGSPESVQAGYRHFGHEPAGSDADMARAHTDRFLRAIEGAPIALPDLQAP
                     GPRPAAGGPVPITPQSPGLRQRVWWGAGSLGTAQWAATQGMQLMSSTLLLEDRGIPFD
                     RLQREQVDAFRAGWAGAGWSWPSRVSVSRSIVPLVDDTTRAYFGRGRRGSEGVGQIEG
                     VTARFGPSFVGEPEELVARLRQDAAVEAADMVLVTVPNLLGVDFNARLLQGVHEIGRE
                     LGWND"
     gene            complement(7942..8622)
                     /locus_tag="VFQ96_07555"
                     /pseudo
     CDS             complement(7942..8622)
                     /locus_tag="VFQ96_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
     gene            8740..9978
                     /locus_tag="VFQ96_07560"
     CDS             8740..9978
                     /locus_tag="VFQ96_07560"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0257679.1"
                     /translation="MPSRAAQVSDEDTAAIRPASAGGAGVGRVVTVLMAGTFLMGTTE
                     FLLAGLLPEIAADFHTSVARAGLTVTVFAAGMIVGAPTVSLFTLRLPRRWTLALALGL
                     FALGHAIAALTGSFDVVLAARFLTGVATGAFWSVGSAVAARVAGPAARSRTLGLVLGG
                     GMIANVVGVPLGAFAGQAIGWRSTFWALAGLAVVAGLAVALAVPANRGPATTPSLRAE
                     FRVLLDGRFWLVLAMCGMVNGAVLSMYSYLAPMTRRAGLPDSAMPFILMAFGAAALIG
                     SIAGGRLGDRHPYLAITLTASVSLVAAAGVAAFGAHPVALVLFFSLLGLVGLSSNPAL
                     VSLALRFGGAADTMASAMPASTFNLGTAIGTGVTGAALATSLGAFSPPLVAAVAATLI
                     FGPLAVLLARDRRTQTRRAS"
     gene            10027..10488
                     /locus_tag="VFQ96_07565"
     CDS             10027..10488
                     /locus_tag="VFQ96_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0257680.1"
                     /translation="MGIVTAALVTGAVLVVATVAGLVWRAHTGRVTDVSGRAESELTG
                     LLGFVSGPAPAEPGTAATLLQFSTDHCAACGPTRRVLGRVASEHDGVTHLDINLTPYP
                     ELARRLNIMQTPTTFLFDGAGRVRGRIGGAPRLPELLSALTPILEADHVHS"
     gene            10475..11002
                     /locus_tag="VFQ96_07570"
     CDS             10475..11002
                     /locus_tag="VFQ96_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEU0257681.1"
                     /translation="MSTAESLSGQSTSPKAPAGARRGIDPRGPRFTASVTAVALLVVL
                     FLGLAGASLAATILVAVVAAVFAWAAAAGVQRHPVGAIFRLLVRPRLAPPQELEDPAA
                     PRFAQLVGLIVTGVGLILGLVGVPYAVPVAAAMAFIAAFLNAVFGYCIGCQLYVLLLR
                     AGVIKAGVPQAGIAA"
     gene            complement(11145..12095)
                     /locus_tag="VFQ96_07575"
     CDS             complement(11145..12095)
                     /locus_tag="VFQ96_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0257682.1"
                     /translation="MTELAGLDVFPLALGGNVFGWTADRDESFRILDAFVDGGGTLVD
                     TADSYSHWVPGHTGGESETIVGQWLAARRPRNVVVATKVSQHPQFRGLSAATVRRAAE
                     ASLRRLGVPAIDLYFAHFDDPQTPLEETVRAFDALVTDGLVRRVAVSNYSAARIREWL
                     AIADRLGAARPVAIQPHYNLVHRNEVEGSIIPLAEEEHLALLPYAGLASGFLTGKYRT
                     TDPAGQASPRAQGAAKYATAQGLAVLDVLDRVAAAHGVSDTAVALAWLRTRSAVAAPI
                     ASASQASQVADLLAGARISLDAAEVDRLDRVSQWTPAGAR"
BASE COUNT         1701 a         4544 c         4351 g         1911 t
ORIGIN      
        1 catcatcgag catcgggggg cctggtgacc ccgccgcgtc gcgagactgg gcccgtgacg
       61 gcgtggcgcg cgggcgggct gcgcggcctc ggcggccgct gaccgggtgg tcggtctaag
      121 ctgaccggcg ggagggccgc gatgcattcc acgggtggcg caccacaaag gcggcgcccg
      181 acgcggcccg gcggccgggt gcggccgcac ggcatggcat ggccggtggg gtgctgggtt
      241 ttcgtcttga cggcgatcgc cgtcgtgcag gtggtgcggg cccagtggtt cgacgccgcc
      301 gtcttctttc tcgcctcggc cggcgtgagc ctcgacgccg tgttccggcc acgctttcac
      361 ccgcggcgca gcctgccgcc gggcgttctc gcggcggcgg ccctcgtcgc gggcctcgtg
      421 atcgcggccc tcccgcggca cggcgccctg atgcgcgcca ccgtcatcat cgccggaatc
      481 gcggcggtgg ccgtgggttg ggcgggctgg gcgatccgcg cgccgcaggc gggcgtcgcc
      541 ttctcgccgg gcgtgcgggc gctcgccctc acctgggcgg cgatcatggt cgcaggctgc
      601 ctgtgggagt tgttccagtt cctgatgagc gtggcagagc cttccggcgc ctggttctcg
      661 ctgagcgacc tcctgaaccc gttcgtcggc cagtgggtgg gcaaggccgc cttcacggcc
      721 gcctggctcg tgtgtggcgt ctggctcctg cggcgcggcg gccggcgggc cgagcggtga
      781 agatactcag ctggacgatg ttcctcttgg ccggcgcggc cctcgtcgtc ggccgggccc
      841 gggtgcgggc tcaccatgcg gatgcgaccg gcagggcgct cttcgaccgg gcgatggccg
      901 accggcgcat ccgcgtggct ctcatcgtca tctggtggtg gttgggctgg cacttcctcg
      961 ccggccagac gctctgaaca cccggcatgc caggccgacg gcgccgacgg ctagggtgag
     1021 agagtgagcc gcgggcaacg gccacgggac gggaggctgt tctccatgag acggtggatc
     1081 tggcgcaaat acttcatcgc ggtggcagcc gggctcgccg cggcgctgtc gtcgacgttg
     1141 gtgaaccgcg gcgcggacat caccatgctc gtgatggcgg cgctcggcct gctcatcgcg
     1201 ctcttcggct tcatcggggt cttcgcgatc ttggagccac gcctcgggcc gtcgggcctg
     1261 cccgtcgcgc tcttcggcgt cttgctcttt ctcgcgccgt gggtcatggg ctacgtgccg
     1321 cacgccgggc aggcctggac ctcgtgggtg tgcggcctcg tcggccttct cgtcggcctg
     1381 tgggggatcg ccggcacccg cggcaagccg ctgccgccgc gcacccagcc acgccgcgat
     1441 cgcggcagcc ggcgccccgc ggcggcctga ggcagcgccg gccgttcgac cgggcctgtc
     1501 gcctactgcc gcggccgggt gcttcccccg gacggcggcg cgggcaggcg cacccgatgg
     1561 gcatcgccca gccttcgcag aaggcgccgc cggcccgcgt tccgggttct cacaagcggc
     1621 ccgtacgggg catgacgagg cgccgccacc gacatgagct cctggtaggc cgtgtccgcc
     1681 ttgcgcgcag cgtcgacgtt tgcggggccc gtcgacatcg ccctcaccgc ctcctggtaa
     1741 cgggtcgcgg cgacggtgac gaccctgccc tgacgcagcg gcaggatgag gacgaccgcg
     1801 gcagccgtca cggcggcgcc caacgctgtc tcccccagcc tgagaagaag aaggccgtcg
     1861 gtgaactcgt ggaactcgac gtagagttcg gcgacagcga ccgtgatgcc gacgatcatg
     1921 aggctgtagt tgacccgcat caggtaaagc ccgacgaaca gcgacaccag gacgaccgcg
     1981 atgaccggtg ccgggcgaga acccgccagg tgggcgacgg gaacggcgat gatcgcgccg
     2041 accagggtgc ccgcgacccg gtagacggct ttgcgcacct gctcccccgc gtggttcgtg
     2101 cccacgagga tgaagaacgc ccccagggcg gcccagtaga accggtaccc cgacaggagg
     2161 ctgccggcga cgatggcggc cgcgcccgcc accgccatct gcaccgccgc cctgacgtgc
     2221 ggagacaaca cggtcgccga cccctcaccc ggggtcacag aggcggcccg gctcaccaag
     2281 gtcgcaccgg gcagccagcc ggcgaacaag accaccgggg aggcgctcac ctgttcgagc
     2341 gaccgcggcc tgtcggcggc atcccgccac gcgtgtgccg ccgccgacac ctggccgaga
     2401 ctctccaccg ccggggcccg ccgggtctcg taggcctgga gcgtggccag cacccggtcc
     2461 aaccgtcgca ggtcgcctct cgccgcggcg cgaaccccgg aacgcagcag tctcgtgaga
     2521 agctggtccc gttccggcca cgcaccgccc tggcagaggg ccgaggcgaa acgacgcacc
     2581 gcctgctcgt ggccgaggag cagccgccgg ccgaggtccc cggcgggaag acccggaacg
     2641 tcgtggtgca ggcccgccag gtggaagtcg acgatcaagg ccgcctcacc ggccagatcc
     2701 acccggcgca tcagcgtata agacgcgtcg ggtcgggccc ctcccgcgcc gagaatcctg
     2761 tcgcaggcag cgaacacccg ccagctgcga gccacgtacg agcggaccgc ccggcgggcc
     2821 atccgggcgg cggtcgggaa gaagacggtg acctgtacga gaaggccggc tgcgacggcg
     2881 acgacgacct cgacggccag ccacccgaga tccgccgggc cgatcccggc gtgctcccgc
     2941 aggaagaagc cgaggaagta gccgatgaat cccaggatgc cgccctggaa accgtgcggt
     3001 cctgcccggc gggcgtacgt gccggcggcg aagacgaaca ccaagacgat gagcgtggcc
     3061 gtgtgccagt cgccgagcac ggagccgagc gcgaggccgg ccgtcaacgg gatcggcatc
     3121 cagccgaaat cggtgagcgc cttcctccag ccgctgaccc ccgggccggt gaaccccgac
     3181 atcatgccga tcatgatccc gagcatcagg ccgatcaaca gggtggcgtg gtcgaaaaac
     3241 gccacgcgag ccgccgcggc agaaggcccg cccgcgggca cgggaaactg cagggcgtgg
     3301 gtgaggcgaa tgaagaacca ctccgacgcc atccccacca tcatggtgag aaccgcttgg
     3361 ctcgccgagc gcagccgccc caagccgacg tctgacgccc atacccatgt ggcgtgccgg
     3421 cccggcatgc tcagcctgaa gggcggcgac agccgcatcc tgcgtgggcc ttggccggcc
     3481 ggcgccgccc ggtggcgggc cgccccttcc cgcaggctct gctgccgacc gaccgtactt
     3541 ctcacctcga gcatccgagg gtcatcactc ccctcaacca gcgcgtccga acctcgcccc
     3601 cgccccgctt cgtcgtctgc agacggtgga aactccgggt gggcgccgtc gccgggggct
     3661 acgaagccag cctaatatcc gacaccgccc cgacaccgcc tagacgaccg ctgcctcggg
     3721 acaccgtgcc tcgtccccgt agactaggcg gagtggggac cagacgcgcc tgggtggtct
     3781 acagtgtcgg cgtcttcgcc tacatcatct cggtgacaca gcgcacgacg atcggcgtgg
     3841 caggcgtcgc cgccaccgac cgcttccggg tggatgcggc gaccctctcc agtctcgccg
     3901 tgctgcagct ggtggtgtat gcggccatgc agataccggt cggcgtgttg atcgaccgat
     3961 acgggccgag aaggctcatg gtgaccggca ccctcctgat gctggtaggg caggtagcgg
     4021 tcgcgttcgc cccgacgatc gcgctcgcgg tggccggacg ggtcctcgtc ggcggcggcg
     4081 acgcgatgct gttcacgtcg gtgatgcgcc tgacggcgtc gtggttccac ggccatatcg
     4141 tcgcccagct ctcacagtgg atcggcaacc tcggccaggt gggtcaggtg ctctccgcca
     4201 tcccgttcgc cttcctgctg cacgagaccg gctggagcgt cgcgttcctc tccgccgcgg
     4261 gttgttccct cctcgccgtc gtgctcgtgc tcgccgccat ccacgatcgc ccggcggggc
     4321 tgcctgcgat cacccggccg atcacgctcg ggcggtcggt ggagtcgctg cgtgcggcgc
     4381 tcgcccggcc cggcacgcag ctcgggttct ggtcgcattt cagcagccag tcggccggct
     4441 cgatcttcag cctcatgtgg ggctacccgt tcatggtgta tgcgctcggc taccggccgc
     4501 aagacgcggc ggcgctcctc atcgtgccgg tcggcgccgc tgtcttcgcc ggccccgtcg
     4561 tgggcatcct ctcggcccgg ttccccctgc gccgcagcaa cctggtgctc ggctgtgtcg
     4621 ctctgatggg cgtcgcctgg gcggccgtgt tggcctggcc cggccagccg ccgttcggcg
     4681 tgatcctctt catgctgatc gcgatcggca tcggcgggcc tggctccgtg atcgggttcg
     4741 actatgcgcg catgttcaac ccggcacgca gtctcggctc cgcgaacggc gtcgtcaaca
     4801 cgggcggttt cgtcgcgagc gtcgcgatga tctttctcat cggggtgatc ctcgacgcct
     4861 acaacgccgc ggcgggcggc ggcgggccgg aacacctcta ctcgatgtcc gccttccgcg
     4921 tggcgttcct cgcccagttc ctcgtcggcg gcatcggagt cacgttcttg ctgcgggtgc
     4981 gccagcgcac ccggcggggc ctggcagaag acgaaggcat cgcggtcggc ccgctctggg
     5041 aggctgtcgt acgctcgtgg cggcggcgca aactccgctg aggcggccgc acggcgggcg
     5101 cggcccgcga ccgaccggat ttactcgacg aacgcacagc gggcgtcgaa aggatcgaag
     5161 acgccgtgcc ggcgggtcgg gggcggggtc cgaaggcgcc gggcacggca ccggcgacgc
     5221 gcgggcgggc gggcggcctg gccgcgcccg gcgcgagcca cggcacactc aggaggagac
     5281 atggttctgc aggcaacgct cacgatcgtg gtcttgatgg ggggaatgat cgggatcgtc
     5341 gtgctgggca tgctgtggca gaaatttacc gcgctgcctc gcaagccgcg cgggcgcacg
     5401 gccaagaccc gaaacaccaa gactcccgtc cgtgtccgcg aggctcgtac cggcgaggcc
     5461 cagcgaaacg gctcccgggg aagcgagact cgtgagagcg gcacccgcac gccggccgcc
     5521 cccgaccagg ccacgcccct cggcggcgcg tcggggccgg gcgagcggct gccgggaggc
     5581 acgggtgacg acacgaccgg caccgacccg agctgagagg cgccgcacag cccccgcaga
     5641 cgacggaaag acgaatgccc gagcgtcaga cggcgcgaag cccgaccgcg tcgaggagtt
     5701 cacgaaggtt ggccgtcagc ggcagtgtgg cgaactcgcg ggcgccggcc cacaggagtt
     5761 ctgccgcatc gtcagccgcg cgcggctcgc ccaccatgtt tctggccaag aaggtgtgaa
     5821 tctcgaagat ccgccggtcg ccgtctggca cccggatcgt ccagagctca cgtctgacgt
     5881 cgatgtcgag acccgtctcc tcccgggtct cccggcacgc cgtcgcccgc ggatcctcgc
     5941 cgggcaacgc cttgccgccc ggcagcgacc agcggccctt gttcggttcg tggccgcgcc
     6001 gcaccagaag gatgcggccc ataccgtcgc ggatgaccgt gctggcggcg acgacaagcg
     6061 gtggcgcaga aggcatgacc ccttcttact cccgctcccg ccccacgggc atcgtcgtca
     6121 tgacggcctg ctgtcgcctc agcgacgggg cctgcgcaga acgtgccgca cgaggagcac
     6181 ggcgatggcc gccgtcacga cgcctccgcc gacgacccac gggactgctg ccgcgtcgtt
     6241 cgagttcggg gcgacgaccg acgcggcgac catcactgcc gacgccgcgt gcacaccggc
     6301 aacccccgac cagatcatcc gccgcaccac ctctttcgct tcgcgcgtca cgcacgacgc
     6361 ctcccaccct cgcgcgccga gttaagaaaa agatgtggtc cgcccgagac ggccgcggct
     6421 cacgccacga tgtcgacttt cgggtcgaac gcgacgtacc cggagaagtc tttgccgacg
     6481 cgttcgaatg cgctcgggta cggcgtcggg tagctccagg ccaggtcttt gcgatccccg
     6541 tcgctgccct gcaccgtgaa gtattggcac acgcctttcc acgggcaggt gtagggcgtg
     6601 tcgctcggca cgaaccggtc ggccgcgaga ctcgccggcg ggaaatacca gttgccttcg
     6661 atgcggatga gctcttcttg tggcgcctcc gccaggacga cgccgtctac gaccgctttc
     6721 atgctccgta caacgcacag gccgcgccgt ggattccccg ccgcaccctc gcggcccggc
     6781 acgcacgggc tgagggaggg ccgagcgtca gtcgttccag ccgagctccc gcccgatctc
     6841 atgcacacct tggagcagcc gcgcgttgaa gtcgacgcct aagaggttcg gcacggtgac
     6901 caacaccatg tctgccgcct cgacggcggc gtcctgccga agcctcgcga cgagctcctc
     6961 gggctccccg acgaacgacg gcccgaaccg tgcagtgaca ccctcgatct ggccgacgcc
     7021 ctcgctgccc cgccgcccgc ggccgaagta cgcccgagtc gtgtcatcga cgagcggaac
     7081 gatgctgcgt gagacagaga cgcgcgacgg ccacgaccag ccggcgccgg cccagcccgc
     7141 ccgaaaggca tccacctgct cgcgttgcag cctgtcgaag ggaatccccc ggtcttccag
     7201 aagcagggtc gacgacatca actgcatgcc ctgcgtggcc gcccactgcg cggtcccgag
     7261 gctgccggcg ccccaccaga cgcgctggcg caggcccggg gattgcggcg tgatcggcac
     7321 gggtccgccg gccgccggcc gcggtcccgg cgcctgcagg tcgggcaggg cgatcggtgc
     7381 gccctcgatg gcacgcagaa accggtcggt gtgggcccgg gccatatcgg catcgctgcc
     7441 ggccggctcg tggccgaagt gccggtagcc ggcctgcacc gattccggtg agccgcggct
     7501 gatgcccagc tgaaggcggc cttcggagag aaggtctaat tgggccgcct gctcggcaag
     7561 gtacagcggc gtctcgtacc gcatgtcgat gacgccggta cccatctcga gggtcgtcgt
     7621 cttggcggcg acggccgcca tcacgacgaa gggcgaggcc tgctgcgagg cgaaatgatg
     7681 cacccggtag aacgcccccg cgataccgat ctcttgcgcg gcgacggcaa gttccaccga
     7741 ctgcacgagg gagtcgcgcg cggtgcgcac gagagacccg gcgccttcgt tccagaaccc
     7801 gaacgacaga aacccgatct tcttcatgcc gcgccctctc ccgttccgga ccgccgtcgc
     7861 ccggcccgca cctcagacgt tagcatcggc cacccgccgc ctcgaggcgg cgccgagacg
     7921 gcccccccgg tcgcgctcag atcacatgtc gcggtaacgc ttggctgccg ctaccgcctt
     7981 ccgaaggccc tcagcgtcta acgccgtcac ataccccggg gtgtcgcgct gaatgcgcca
     8041 gccctccgcc agcggcccga gatccaccgt gtcgaacccg aactcatcga tcagcgcccg
     8101 cacacgatcc ttcgccgccg catcgtcgcc cgcgacggcg agcgcgcgcc ggccgggcgt
     8161 gcccggcgcg gtgcccgcgg tcgtcaagtc gggtgcggga atgtggttga acgccttgac
     8221 gacgtgagcc ccgacgagac gcgcttgcag aagctctgca gtcgtggtcg tctcgtcgtc
     8281 gagcgccggg atgtgcccgt cgcgctgcgg gtagtagttg ttggtgtcga tgaccgtctt
     8341 gcccgccaac ggctgggcgg gaacctgtcc gacgttcttc aacgggatgg tcacgacgac
     8401 gatgtcgccg gcccgtgccg caccctccgc cgtgtcggcg cgggccgtgc cgcccagttc
     8461 tgccaccagc cccgcgaggc tctgtggcgc gcgcgagttg ctcaccacga cgtcatagcc
     8521 gtgcgcgacc gcgagccttg ccagttgaga accgatgtga cctgccccga tgaatccgat
     8581 tgtcgtcata cttgcgacaa cgcgggcgct cgcccggttc ttccgccacc cgcccgggcg
     8641 ttacggcgta tgacgacgcc cggcccgccc cacagggaat accgtggatc cagcagtgcg
     8701 atggacaggc ggcacgtcat ttcggaaagc gagtttttca tgccctcacg agccgcccag
     8761 gtttccgacg aagacaccgc ggccatccgg ccggcgagtg cgggcggcgc gggagtgggg
     8821 cgcgtcgtca ccgtgctgat ggccggaacc ttcctcatgg gcacgacgga gttcctgctc
     8881 gccgggcttc tgcctgagat cgccgcggac tttcatacga gcgtcgcccg cgccggcctg
     8941 acggtgacgg tgttcgccgc cggcatgatc gtcggggccc cgacggtctc gcttttcacc
     9001 ctgcggctgc cgcgccgctg gaccctggcg ttggcgttgg gcctgttcgc gctcgggcac
     9061 gccatcgccg ccctgaccgg cagcttcgac gtcgtcttgg ccgcacgatt cctcaccggg
     9121 gtggcgaccg gagcgttctg gtcggtgggt tccgcggtcg cggcacgggt ggcgggcccc
     9181 gccgcacgct ctcgcacgct cggcctggtc ttgggcggcg gaatgatcgc gaacgtcgtc
     9241 ggcgtgcctc tcggcgcgtt cgccggccag gcgatcgggt ggcgcagcac gttctgggcg
     9301 ctcgccgggt tggccgtcgt cgccgggctg gccgtcgccc tggcggtgcc ggcgaaccgc
     9361 gggccggcga cgacgccttc gctgcgcgca gagttccggg tcctcctcga cggccgcttc
     9421 tggctggtct tggcgatgtg cggcatggtc aacggagccg tgctgtcgat gtacagctac
     9481 ctggcgccga tgaccaggcg tgcgggcctg cccgattcag ccatgccctt catcctgatg
     9541 gccttcggcg cggcggcgct catcggcagc atcgcgggcg ggcgcctggg cgaccggcat
     9601 ccctaccttg cgatcacgtt gaccgcgtcg gtgagcctcg tggctgcggc gggcgtcgcc
     9661 gcgttcggcg cccacccggt cgcccttgtg ctgttcttct cgcttctggg gctcgtcggg
     9721 ctctcttcga accccgccct ggtgtctttg gcgctccgct tcggcggcgc ggccgacact
     9781 atggcgtcgg cgatgccggc ctcgaccttc aacctcggca cggcgatcgg caccggcgtg
     9841 accggggccg ccctggcgac gagcctcggc gccttctcgc cgccgctcgt cgccgccgtc
     9901 gcggcgaccc tcatcttcgg gccgctcgcc gtcttgctgg cacgcgaccg tcgcacccag
     9961 acgcgccgag cgtcataaag cgacacagac ccggggaggt cacagccgag cgataggttc
    10021 ggtgacatgg gaattgtcac agccgcgctc gtcaccggcg cggtgctcgt cgtggcgacc
    10081 gtggcgggtc ttgtttggcg cgcccacacc ggccgcgtca ccgacgtctc cgggcgggcc
    10141 gagtcggagc tcaccggtct cctgggcttc gtctccggcc cggcgccggc agagccgggc
    10201 accgcggcga cacttctgca gttctccacc gaccactgcg ccgcctgcgg cccgacgagg
    10261 cgggtccttg gccgggtcgc ttcggagcat gacggcgtga cacatctcga catcaacctc
    10321 acgccttacc cggaattggc gcgacgcctg aacatcatgc agaccccgac gacgttcctc
    10381 ttcgacgggg ccggccgggt gcggggtcgt atcggcggcg ccccgcgcct tccagagctt
    10441 ctttccgcgc tgacaccgat tctggaggcc gaccatgtcc acagctgaat ctctctcggg
    10501 gcaaagcacg tcgcctaagg cgccggccgg cgcccgtcgg ggcatcgacc cgcgcggccc
    10561 gcgcttcacc gcctcggtga cggcagtggc ccttctcgtc gtgctgttcc tcgggctcgc
    10621 cggcgcaagc cttgccgcga cgatccttgt ggccgtggtg gcggccgtct tcgcttgggc
    10681 ggcggcggcc ggggtgcaac gccaccccgt gggtgcgatc ttccgcctcc ttgtgcggcc
    10741 gaggctggct ccgccgcaag agctcgaaga cccggcggcg ccacggttcg cccagttggt
    10801 cggcctcatc gtgacgggcg tcgggctcat cctcggcctt gtcggcgtac cgtacgccgt
    10861 gccggtggcg gccgcaatgg cgttcatcgc ggccttcttg aacgcggtgt tcggttactg
    10921 catcggctgc cagctctacg ttctgctgct gcgcgccggc gtcatcaagg cgggcgtccc
    10981 ccaggcgggg atcgccgcgt aaaggcagca ctcgcttcgt acgccttgcg tggcccggcc
    11041 tcgcccacaa ggcctctggt acccatccgc cccggccgcg tcacaccgtt cgttccggcc
    11101 ggggcgggtg ctcttctcgc gcccgttctg ccgcccgcgg accgctacct tgccccggcc
    11161 ggagtccact gcgacacccg gtccaaccgg tcgacctctg ccgcgtcgag gctgatgcgg
    11221 gcaccggcca acaagtccgc cacctgggac gcctgcgagg cgctcgcgat cggggccgcg
    11281 acagcactgc gggtccgcag ccaggcgagc gcgaccgccg tgtcggagac gccgtgcgcc
    11341 gcggcgacgc gatcgagcac gtcgagaacg gcgagcccct gtgcggtcgc atacttcgcg
    11401 gctccttgtg cgcggggcga ggcctggccg gcggggtcgg tcgtgcggta tttgccggtc
    11461 aagaacccgg aggcgaggcc ggcgtagggc agcagcgcca gatgctcctc ctcggccagg
    11521 gggatgatcg agccctctac ctcgtttcga tgcacgaggt tgtagtgcgg ctggatagcg
    11581 acgggtcgtg ccgcgcccag ccggtcggcg atggccagcc attcgcggat ccgcgccgcg
    11641 ctgtagttgg atacggcgac gcgcctgacg agcccgtcgg tgacgagcgc gtcgaaggcc
    11701 cgcacggtct cttcgagcgg ggtctgcggg tcgtcgaaat gggcgaagta caggtcgatg
    11761 gcaggcacgc cgagccgccg taaggaggcc tcggccgccc ggcgcacggt cgcggcggag
    11821 aggccccgaa actgtggatg ctggctgact ttggtggcga cgacgacgtt ccgggggcgt
    11881 cgggctgcga gccactggcc aacgatggtc tcactctcgc cgccggtgtg acccggcacc
    11941 cagtggctgt aggagtcggc ggtgtcgacg agcgttccac caccgtcgac gaacgcgtca
    12001 aggatgcgga acgactcgtc gcggtccgcc gtccagccga acacgttgcc gcccagggcg
    12061 agagggaaca cgtcaaggcc tgccaattca gtcacgacat gaccaacctc gccacccgac
    12121 cccgctattc cgccatcccg gccgccgaag atcgacatca tgcctcggcc gggtactccg
    12181 gcaggccgcc gccggtctcc tcccctcgcg ggcgccgagg cttggggtcg ggcacggacg
    12241 tctcgttgat ccggacggtt gcccgccggg tcccggaagg cgcagtcccg tggcccccag
    12301 ggctgtttca taggctcctg aaccacctcc gcgccggcgt cgcggatccg cgcgaacgtc
    12361 gcgtcgagat cgtgcgtggt gaagacgaat gggatcgcgg cctcagccgc gagccctcct
    12421 gttgccgcgg ccccgggagg cctgcgcccg gggagccacc accccgcggc tcgccgtcgt
    12481 ccacaccggc gtgtgctcgt cgcggag
//
LOCUS       DASTNL010000235         6436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_363079, whole genome shotgun sequence.
ACCESSION   DASTNL010000235 DASTNL010000000
VERSION     DASTNL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363079"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFQ96_07580"
     CDS             complement(<1..375)
                     /locus_tag="VFQ96_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0257683.1"
                     /translation="MAGDSFVHLHVHSEYSMLDGAAHIGPLIDAVRAEGMPGIAITDH
                     GNVFGAYDFWKAASAAGVKPIIGTEAYLTPGTNRADHTRVRWGSGGGDDVSGSGAYTH
                     MTLLAETTEGMHNIFRLSSRSSL"
     gene            complement(454..1377)
                     /locus_tag="VFQ96_07585"
     CDS             complement(454..1377)
                     /locus_tag="VFQ96_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0257684.1"
                     /translation="MNAQSRTIVAPEGVEGLRADQALAKLLGFTRSFAATLVTAGSVQ
                     IDGRPARKSDRLRAGSLVEVTWSDERTAAAPPSPVPDLAIVHDDDDIVVIDKPAGVAA
                     HPALGWQGPTVVGALRAAGFVLSSLGPAERTGIVHRLDAGTSGLMVVAKSDRAYSALK
                     EAFREHTVTKIYHTVVQGHPDPLEGTIDGPIGRHPNAQWRFAVVAGGKPSVTHYRTLE
                     AFRHASLLEVRLETGRTHQIRVHMSARRHPCLGDTMYGADPTLGARLGLERQWLHAVR
                     LGFRHPGTGDQVVFDSAYPPDLQHALDLLDG"
     gene            complement(1374..2045)
                     /gene="lspA"
                     /locus_tag="VFQ96_07590"
     CDS             complement(1374..2045)
                     /gene="lspA"
                     /locus_tag="VFQ96_07590"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU0257685.1"
                     /translation="MPNPHRREITRPSPRALATLAIVAVCWYGLDQLTKYLVVTHLPY
                     ERSVDVIGTLLQFFYITNSGAAFSIGSGSTWIFSIIGAGTLLFIVWYARRIRSLAWAT
                     LFGLLLGGLCGNLSDRLLRPPGFGLGRVVDFIKIPLLPAYFNIADAGITASMALFIIL
                     TIRGVGLDGRVERLARVTDDAAVTGTPGTAAPDAQHSGTRPPDGPTAEATRPRDDTAG
                     GPAGS"
     gene            complement(2038..2754)
                     /locus_tag="VFQ96_07595"
     CDS             complement(2038..2754)
                     /locus_tag="VFQ96_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU0257686.1"
                     /translation="MALTPEDVVNKRFQPTKFREGYDQDEVDDFLDEVVVELRRLGQE
                     NEDLRRQLAAGQPDRPETNDEVSDDEDADNDEPAAAEAPAAPKAASHMAPEEEPVPAA
                     TAAPAANEAESANNLLQLAQRLHDEHVKAGQDKRDALIAEGRETAQRLIAEAESEQQE
                     KIATLEGQRTQLEQRVEELRTFERDYRAQLRGYIEGQLRQLDTEKQGGLAPQNPSQAR
                     GTDQKTQGGTQQGAFQGFSA"
     gene            complement(2938..3240)
                     /locus_tag="VFQ96_07600"
     CDS             complement(2938..3240)
                     /locus_tag="VFQ96_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU0257687.1"
                     /translation="MLIVALVATVVYYLLLLFFFLMLARLVLDLVQAFNHSWRPQRFG
                     LVLAEFAYSVTDPPVRFFRKLIPPLRLGPLALDFGWTITMLACMIGMYAVTALFQA"
     gene            complement(3317..3817)
                     /gene="sepF"
                     /locus_tag="VFQ96_07605"
     CDS             complement(3317..3817)
                     /gene="sepF"
                     /locus_tag="VFQ96_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU0257688.1"
                     /translation="MTNAWKKTMVYLGLAEEDIAEEQPGDMPDEAAAGRPETRMPGPH
                     EAPVTPLPTRAPVTPLHKKHQGRNTAVQEMNEILTVHPRQYRDAQAIAESFREGIPVI
                     INLSQMSDADARRLVDFASGLSQGLYGKIERVTSKVFLLSPAHISVSGDAGAGEDTEQ
                     AFFAQA"
     gene            complement(3867..4568)
                     /locus_tag="VFQ96_07610"
     CDS             complement(3867..4568)
                     /locus_tag="VFQ96_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU0257689.1"
                     /translation="MTPGLSERLTVVRERIQAAARLAGRDPAEITLIAVTKFHPASLV
                     RDLAALGQRDVGENRDQEAHAKAGELASLGLHWHFIGQLQTNKARRVVSYAHAVHSVD
                     RPRLVDALDAAVGGENGPANGGPLDCFLQVSLDGSPGRGGVTPDGLLPLAQQVLAAAG
                     LRLRGLMAVAPLGEDPDPAFEHLRLLSLRLRRLAPEANAISAGMSHDFEKAIAHGATH
                     LRIGTAITGIRPTRG"
     gene            complement(4585..5757)
                     /gene="ftsZ"
                     /locus_tag="VFQ96_07615"
     CDS             complement(4585..5757)
                     /gene="ftsZ"
                     /locus_tag="VFQ96_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU0257690.1"
                     /translation="MPTNQNYLAVIKVVGIGGGGVNAVNRMIELGLRGVEFIAINTDA
                     QALLMSDADVKLDVGREITRGLGAGADPEVGRRAAEDHQEEIEEALAGADMVFVTAGE
                     GGGTGTGGAPVIARIAKAIGALTIGVVTKPFGFEGRRRSEQAEAGVARLKEEVDTLIV
                     VPNDRLLEISDRGISMLEAFATADQVLLAGVQGITDLITTPGLINLDFADVKSVMQGA
                     GSALMGIGSSRGADRAIKAAELAVASPLLEASIDGAHGVLLSIQGGSNLGIFEINDAA
                     RLVQEAVHPEANIIFGAVIDDTLGDEVRVTVIAAGFDGGQPVPRESVESNKHRAAFAQ
                     NGLGIALEDTEALSAESLPEADDASWGGGQEPVAVPPTPSDPTFDDDDLDVPDFLK"
     gene            complement(5888..>6436)
                     /locus_tag="VFQ96_07620"
     CDS             complement(5888..>6436)
                     /locus_tag="VFQ96_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEU0257691.1"
                     /translation="ATGSTAAEVRHAVSGQLGRPLASISRSEVHDALAKIDRIGSYSL
                     ALKPPNTMVLRVTQRVPVAAVRHAGGYATVDAAGVSLAQSRARPALPLVTSRVDTKGF
                     AAAIQVIQALPATVDKRVDTVSATSLDNVAFTLRGHHGATVVWGDRSKPALKAKVLTA
                     LLGAVPHAARYDVSSPGDAVTR"
BASE COUNT          919 a         2123 c         2316 g         1078 t
ORIGIN      
        1 gagagacgac cgggaggaga ggcggaagat gttgtgcatc ccctccgtcg tctcggccag
       61 caatgtcata tgcgtgtacg cgccggagcc cgagacgtcg tctccgccgc cggagcccca
      121 gcgcacccgc gtgtggtcgg cccggttcgt gccgggggtg aggtacgcct cggtcccgat
      181 gatgggcttc acgccggccg cgctcgccgc cttccagaag tcgtaggcgc cgaacacgtt
      241 gccgtggtcg gtgatcgcga tgccgggcat tccctctgcg cgtaccgcgt cgatgagcgg
      301 gccgatgtgg gcggcgccgt cgagcatcga atattcgcta tggacgtgca gatgcacgaa
      361 ggagtcgcct gccaccgttc gcctttccgc cgcttgcctg gcacgtaagg cctcggcccg
      421 cggccacccc gtcagcttaa gccgtcgtgc cgctcagccg tcgagcaggt cgagggcgtg
      481 ctgcagatca ggcgggtagg ccgagtcgaa gacgacctga tcgccggtgc cggggtggcg
      541 gaatccgagg cgcaccgcgt gcagccactg tcgttcgagc ccgagccggg cgccgagcgt
      601 cgggtcggcg ccgtacatcg tgtcgccgag gcaggggtgg cgcctcgccg acatgtgcac
      661 gcggatctgg tgggtgcgcc cggtctccag ccgaacctcg aggaggctgg cgtgtcggaa
      721 ggcctcgagg gtgcggtaat gcgtgacgga gggcttgccg ccggcgacga ccgcgaagcg
      781 ccattgggcg ttcggatggc ggccgatcgg accgtcgatc gtgccttcca atgggtcggg
      841 gtgcccctgc acgaccgtgt ggtagatctt cgtgaccgtg tgctcgcgga acgcctcttt
      901 caacgccgag tacgcccggt ccgacttcgc caccaccatg agcccgctgg tgccggcgtc
      961 gaggcgatgc acgatgccgg tgcgttcggc aggccccaag gacgagagga cgaagccggc
     1021 ggcccgtaac gccccgacga ccgtcgggcc ctgccagccg agggcggggt gggccgcgac
     1081 gccggcaggc ttgtcgatga cgacgatgtc gtcgtcatcg tgcacgatgg cgaggtcggg
     1141 caccgggctg ggaggggcgg ccgcggtgcg ttcgtcgctc caggtgactt cgacgaggct
     1201 cccggcacgc agccggtcag acttgcgggc gggccggccg tcgatctgca cgctgccggc
     1261 ggtgacgagc gtcgccgcga aactgcgcgt gaatccgagc agcttcgcga gagcctggtc
     1321 ggcccgcaga ccctcgaccc cttcgggcgc gacgatcgtg cgcgactggg cgttcacgac
     1381 cccgccgggc cgccggccgt gtcatccctg ggtcgtgtcg cctccgccgt cggtccgtcg
     1441 ggtggccggg tgccggagtg ctgggcgtct ggggccgcgg tgccgggcgt gcctgtcacc
     1501 gcggcgtcgt cggtgactcg ggcgagacgt tcgacgcggc cgtcgaggcc gacgccgcgg
     1561 atcgtcagga tgatgaagag cgccatgctg gcggtgatcc cggcgtcggc gatgttgaag
     1621 taggccggca gaagcgggat cttgatgaag tcgacgaccc ggcccagtcc gaacccgggc
     1681 gggcgcagca ggcggtcgga gagattgccg cagagcccgc ccagcagcag gccgaagagg
     1741 gtggcccagg ccagcgagcg gatgcggcgg gcgtaccaga cgatgaacag gagcgtgccc
     1801 gcaccgatga tcgagaagat ccaggtggat ccactgccga tcgagaaagc cgcgcccgag
     1861 ttcgtgatgt agaagaactg cagaagggtg ccgatgacgt caacggaacg ttcgtacggc
     1921 aggtgtgtga cgacgaggta tttcgtcagc tggtcgagcc cgtaccaaca cacggcgacg
     1981 atggccagcg tggcgagcgc ccgcggactc ggccgtgtga tctcccgtcg atgcgggtta
     2041 ggcactaaac ccttggaacg cgccttgctg ggtgccgccc tgagtcttct ggtcggtgcc
     2101 gcgagcctgt gacgggttct gcggggcgag gccgccctgc ttctcggtgt cgagctggcg
     2161 caactggcct tcgatgtaac cgcgcaactg agcgcggtag tcgcgttcga acgtgcgcaa
     2221 ctcttcgacc cgctgctcga gttgggtgcg ctgcccctcg agggtggcga tcttctcttg
     2281 ctgctccgac tcggcctcgg cgatgaggcg ctgcgcggtc tcgcgcccct cggcgatgag
     2341 cgcgtcacgc ttgtcttggc cggccttgac gtgttcgtcg tgcaggcgct gcgccaactg
     2401 gaggaggttg ttggcgctct cggcttcgtt ggcagccggc gccgcggtgg ctgcgggcac
     2461 gggctcctct tctggcgcca tgtggctggc ggccttcggc gcggccggag cctcagccgc
     2521 ggcaggctcg tcgttgtctg cgtcttcgtc gtcggagacc tcgtcgttgg tctcggggcg
     2581 atccggctga ccggcggcca gctgccgccg caggtcttcg ttctcttggc ccagtcgccg
     2641 caactctacg acgacctcgt cgaggaagtc gtcgacctcg tcctggtcgt acccctcgcg
     2701 gaacttcgtc ggctggaatc gcttgttgac gacgtcttcg ggagtcagcg ccatgatgtg
     2761 ccacccttac aagtttggtt gcgaatacgg ctcattcgag agctaacgtt agcaaatcgg
     2821 tctgaggttc ctggttccct gcggcccgag tctcgccccg ccccggccgc ccgggactgc
     2881 cggccgggcg gggtgccggg cggatgtgtg cccatcaggt tacatcggcg aagcccgtca
     2941 ggcctgaaac aacgccgtca ccgcgtacat gccgatcatg cacgcgagca tcgtgatcgt
     3001 ccagccgaaa tcgagggcca acgggccgag ccgcagcggc ggaatgagct tgcggaagaa
     3061 gcgcaccggc gggtctgtga ccgagtaggc gaactcggcc aagacgaggc cgaagcgctg
     3121 cggccgccag gagtggttga acgcctggac caggtcgagc acgagcctcg cgagcatcaa
     3181 gaagaagaag agcaggagaa ggtagtagac gacggtggcc acgagcgcga cgatcagcac
     3241 accacaagta tctcaatgac agcggtgcgt tccgtgccgc acccgtggcg tgccggcggc
     3301 cgcgtgcgcc ggcgggttat gcctgcgcga agaacgcctg ctccgtgtcc tcccccgccc
     3361 ccgcatcccc cgagacggag atgtgtgccg gcgagagcag gaacaccttc gaggtgacgc
     3421 gctcgatctt gccgtacagc ccctgggaga ggccgctggc gaagtcgacg aggcggcgtg
     3481 cgtcggcgtc gctcatctgg gaaaggttga tgatcaccgg gataccttcg cggaacgact
     3541 ccgcgatagc ctgcgcatcc cggtattggc ggggatgcac cgtgagaatc tcattcattt
     3601 cttgaaccgc cgtgttccgt ccctgatgtt ttttgtgcaa cggcgtcacg ggggccctcg
     3661 tgggcagcgg cgtgacggga gcctcgtgcg gccccggcat gcgcgtctcc gggcgccctg
     3721 ccgcggcctc gtcgggcatg tcgcccggct gctcctcggc gatgtcttct tccgccaggc
     3781 cgaggtagac catggtcttc ttccatgcgt tcgtcatgct gtcctccgtc gcgccggtct
     3841 gcgcgtgcct ttcgttcttt acgaggttaa ccgcgagtcg ggcggatgcc ggtgattgcg
     3901 gtgccgatgc gcaggtgtgt cgcgccgtgg gcgatcgcct tctcgaaatc gtgggacatg
     3961 ccggccgaga tggcgttcgc ctcgggtgcg aggcggcgca ggcgcagcga aagaagccgg
     4021 aggtgctcga aggccgggtc ggggtcttcg ccgagcggcg cgaccgccat gagtcctcga
     4081 aggcgtaggc cggcggcggc gaggacctgc tgcgccaggg gcaggagccc gtcgggcgtg
     4141 acgccccctc gcccgggtga gccgtcgagg ctcacctgga ggaagcagtc gagcgggccc
     4201 ccgttcgccg ggccattctc gccgccgacg gcggcgtcga gcgcgtcgac gagacgcggc
     4261 cggtcgacgg agtgcacggc gtgggcgtag gagacgacgc gacgggcctt gttggtctgc
     4321 agctggccga tgaaatgcca gtgcaacccg agcgaggcca gctcgcccgc cttggcgtgc
     4381 gcctcctggt cgcggttctc gccgacgtcg cgctggccca gcgcggcgag gtcgcgcacg
     4441 agcgatgccg ggtgaaactt ggtcacggcg atgagcgtga tctcggccgg gtctcggccc
     4501 gcgaggcgcg cggccgcctg gatgcgttcc cgcaccaccg tgagccgctc gctaagaccc
     4561 ggcgtcatcg tgaaagcttc cgaatcattt caggaagtcg ggcacgtcga ggtcgtcgtc
     4621 gtcgaacgtc gggtcggagg gcgtgggtgg cacggcgacc ggttcctggc cgccgcccca
     4681 cgaggcgtcg tctgcctcgg gcaggctctc ggcggagagc gcctccgtgt cttccagcgc
     4741 gatgccgagc ccgttctgcg cgaacgccgc ccggtgcttg ttcgactcga cggactcccg
     4801 gggcacgggc tggccgccgt cgaagcccgc ggcgatgacc gtcacgcgca cctcgtcgcc
     4861 gagcgtgtcg tcgatcaccg cgccgaagat gatgttcgcc tccgggtgca ccgcttcttg
     4921 caccaggcgt gcggcgtcgt tgatctcgaa gatgccgagg ttcgacccgc cttgaataga
     4981 gaggagcacg ccgtgggcgc cgtcgatgga ggcctcgagc agcggcgagg cgacggcgag
     5041 ctctgccgcc ttgatcgcgc ggtcggcgcc gcgtgaggag ccgatgccca tgagcgcgga
     5101 gcccgcgccc tgcatgaccg acttgacgtc ggcgaagtcg aggttgatga gcccgggggt
     5161 ggtgatgagg tcggtgatgc cttgcacccc ggcaaggagc acctggtcgg cggtcgcgaa
     5221 ggcctcgagc atgctgatgc cgcggtcgct gatctcgagg aggcggtcgt tgggcaccac
     5281 gatgagcgtg tcgacctctt ctttgaggcg ggccacgccg gcctcggcct gctcgctgcg
     5341 ccggcgccct tcgaagccga acggcttcgt gacgacgccg atcgtgagcg cgccgatcgc
     5401 cttggcgatg cgcgcgatca ccggcgcacc tcccgtgccg gtgccgccgc cctcgcccgc
     5461 cgtgacgaag accatgtcgg ctcccgcgag cgcctcctcg atctcctcct ggtggtcttc
     5521 ggccgcgcga cgcccgacct ccgggtctgc cccggcgccc agcccccggg tgatctcccg
     5581 accgacgtcg agcttcacgt cggcgtcact catcaggagc gcctgcgcat cggtgttgat
     5641 cgcgatgaac tccacgccgc gcaggccgag ctcgatcatc cgattcacgg cgttgacgcc
     5701 gccgccgccg attccgacca ccttgataac ggcgagatag ttctgatttg ttggcacgtc
     5761 cgacctccgc ttgacaaacc ttcaatctca agtagaagat taaggattgc gtgaatatcc
     5821 gattcgctca tctgacgtta tgcgccgggc ggggaactgc cggtagcggc ttccgcgtgt
     5881 cgggaactca ccgcgtcacc gcgtcacccg gtgacgagac gtcgtaacgc gcggcgtgcg
     5941 gcacggcgcc aaggagcgca gtgagcacct tggccttcag cgccggcttc gagcggtcgc
     6001 cccacacgac ggtggccccg tggtggccgc gcagcgtgaa ggcgacgttg tcgaggcttg
     6061 tcgccgacac cgtgtcgacc cgcttgtcga ccgtggccgg cagcgcctgg atcacctgga
     6121 tcgccgcggc gaatcctttc gtgtcgacgc ggctcgtgac gagcggcagg gccggcctcg
     6181 ccctggactg cgcgaggctc acgccggcgg cgtcgacggt ggcgtagccg ccggcgtggc
     6241 gcacggccgc cacggggacc cgctgtgtga cgcgcagcac catcgtgttc ggcggcttca
     6301 acgccaacga atagctgccg atccggtcga tcttcgccaa agcgtcgtgc acctcagacc
     6361 tgctgatgct ggcgagcggc cggcccagtt gacccgacac ggcgtgccgg acctcggcgg
     6421 cggtcgaacc ggtcgc
//
LOCUS       DASTNL010000236         6073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1473646, whole genome shotgun sequence.
ACCESSION   DASTNL010000236 DASTNL010000000
VERSION     DASTNL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473646"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFQ96_07625"
     CDS             <1..325
                     /locus_tag="VFQ96_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257692.1"
                     /translation="VLLRCGVTPPGPTTLHCVDALGIDWVIDDRVDTAIRYTTFGRTP
                     AVQVVLDHTKVSDAAVLGDLKAAVSVIPQTRRCIGLGDTGTNPSAPARPASPTAAPTA
                     TPRRG"
     gene            complement(306..1385)
                     /locus_tag="VFQ96_07630"
     CDS             complement(306..1385)
                     /locus_tag="VFQ96_07630"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEU0257693.1"
                     /translation="MGTVTVALVFGGRSSEHSISCATAAGILAAIDRDRYDVVPVGVT
                     ADGVFVLEQDDPEAFRMSAAGEPPRVTDNGSRVAWPTRAGERVLTVSRPDAPAARVAV
                     DVVFPLLHGRWGEDGTIQGMLDLVDMPYVGSGVLGSALGMDKHFTKTVLRAAGIAVAP
                     WSVVSRARWRAAPHEVHAEALEWGLPAFVKPARGGSSVGVGRVAAADELDSALAAAFA
                     EDDRVLVERQVTGREIEVGVLGGRRGAPARTGAPGEIVVEKGFYDFDAKYRDSGGVRL
                     ACPADLEPAVARQAADLAARAFDVLGLAGLARVDFFLTADGFVVNEVNTMPGFTPISM
                     FPTSWRAVGLDYPSLIDELISLALE"
     gene            complement(1285..2418)
                     /locus_tag="VFQ96_07635"
     CDS             complement(1285..2418)
                     /locus_tag="VFQ96_07635"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0257694.1"
                     /translation="MKRRDAARPARVAVFGAGSWGTTFAKVLADAGADVTVWARRAEV
                     ATELNEAHRNSEYLPGVNLPRRVHATTRLDQALDGAEQVYLSVPSQSLRRGLGLVSGH
                     LDDGSIVVSLIKGVERTTGRRMSEVIAEELRLDPGRIAVASGPNLALEIAREQPTASV
                     VASEDLETAGRVARTAGNGYMKFFVGTDVVGTEFAGILKNLIAVAIGIVDGVGYGENT
                     KAAIITRGLVEMTDFAVAYGAAPETLTGLAGLGDLIATCTSPLSRNNTAGRLLGQGYS
                     YSDVIAQMDQTAEGLTSVKAVLALARQRGVRMPIVRQVGQVLDGTLDPKDIAPHMATE
                     SDEPQGERNPHGHSDGRARLRGAFQRAFDKLRDRRRDSRGHRP"
     gene            complement(2408..3184)
                     /locus_tag="VFQ96_07640"
     CDS             complement(2408..3184)
                     /locus_tag="VFQ96_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0257695.1"
                     /translation="MKADAPARAGRRRRTERARPSAFWILALVILPVISTVTRLRIVD
                     GHKLPPHGACIIVPNHVSELDPVIVGVGVWRLGRLPRFLAKGSLFRVPIVGWLLRRSG
                     QIPVERAAVSRQGSPLDAARLVAERGNAVIVYPEGTLTRDPDLWPMRGKTGAARMALR
                     YGLPVIPVAHWGVQDVLGRYSHRVRLFPPRRIRIVFGDPVDLEAFRGRPIDATTLAEA
                     TEAIMVAITGLVARLRGTTPPTGRWDPAAHDQKETGRFEA"
     gene            complement(3174..4541)
                     /gene="murA"
                     /locus_tag="VFQ96_07645"
     CDS             complement(3174..4541)
                     /gene="murA"
                     /locus_tag="VFQ96_07645"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU0257696.1"
                     /translation="MNTLTTQTLRTSAARLAAPEVVTIRGGRPLEGRIGVHGAKNLVT
                     KAMVAALLGDGPSILKNVPDISDVRIVRDLLEVHGVQVSSGPEGVLTLDPSAVESAHI
                     ADIDAYAGSSRIPILFCGPLLHRLGEAFIPDLGGCRIGDRPINYHLDVLRSFGAVVEK
                     LPSGIRMTAPNGLVGTKVQLAYPSVGATEQVLLTGVRADGITELRGAAIEPEIMDLVN
                     ILQKMCAIITVDTDRIIRIEGVDRLVGYEHTAIFDRNEAASWAAAALATEGDVFVAGA
                     RQSEMLTFLNVYRKVGGAFDIRDDGIRFYHPGGRLKPVVIETDVHPGFMTDWQQPLVV
                     ALTRADGVSIVHETVYEKRFGFVDALVKMGATIQVHKECLGGHPCRFGQRNFDHSAVI
                     AGPSRLHGADITVPDLRGGFSHLIAALSATGRSRVSNVGIIRRGYEDFLTKLHMLGAD
                     FDLEG"
     gene            complement(4764..5369)
                     /gene="leuD"
                     /locus_tag="VFQ96_07650"
     CDS             complement(4764..5369)
                     /gene="leuD"
                     /locus_tag="VFQ96_07650"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0257697.1"
                     /translation="MEKVSVLTGVAVPFRRSDVDTDQIIPAKFLKRITKSGYDDALFF
                     RWRQDPDFILNQEPYRAGKILVAGPNFGTGSSREHAVWALRDYGFTAVLSPRFADIFR
                     GNSGKQGLVTGQVAQEDIERLWEAIEARPGVEARVDLVERTATVGGLTVDFQIDDYMR
                     WRLMEGLDDIALTLQHADQITAYEKTRAAWRPSTLPAFVEN"
     gene            complement(5372..>6073)
                     /locus_tag="VFQ96_07655"
     CDS             complement(5372..>6073)
                     /locus_tag="VFQ96_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU0257698.1"
                     /translation="RPHAPKGALWDEAVAYWDTLRSDPDAVFDKEVDIDADKLEPFVT
                     WGTNPAQGVPMSESVPDPDSFDDPNLRSSAQRALEYMDLKPGTPMKGIPVDVVFMGSC
                     TNSRIEDLRDFASIIRGHKKAPNVRVLVVPGSARVRLQAEAEGLNTLFEDFGAEWRAA
                     GCSMCLGMNPDQLQPGERCASTSNRNFEGRQGKGGRSHLVSPLVAAATAVRGTLSSPW
                     DIERDDLLVGAGTEA"
BASE COUNT          923 a         2136 c         2106 g          908 t
ORIGIN      
        1 cgtgctgctg cgttgcggtg tgacgccgcc cggcccgacg acgttgcact gcgtcgacgc
       61 cctcggcatc gactgggtca tcgacgaccg ggtcgacacc gcgatccgct acaccacgtt
      121 cggacgcacg cccgccgtcc aggtggtgct cgaccacacc aaggtcagcg acgccgccgt
      181 gctgggcgac ctcaaggccg cggtgagcgt catcccacag acccgccggt gcatcggcct
      241 cggcgacacc gggacgaacc cctcggctcc ggcgaggccc gcgtcgccga ccgccgcccc
      301 caccgctact ccaaggcgag gctgatcagc tcgtcgatga ggctcgggta gtcgaggccg
      361 acggcccgcc agctcgtcgg gaacatcgag atcggggtga agcccggcat cgtgttgacc
      421 tcgttgacga cgaatccgtc ggcggtgaga aagaagtcga cgcgggcgag cccggccagg
      481 cccagcacgt cgaacgcccg cgcggcaagg tcggcggcct gcctagcgac ggcaggctcc
      541 aggtcggccg ggcaggcgag gcgcaccccg ccgctgtcgc gatacttcgc gtcgaagtcg
      601 tagaacccct tctcgacgac gatctcacca ggcgcgccgg tgcgggccgg cgcgccccgg
      661 cgcccgccga gcacgccgac ctcgatctcc cggcccgtga cctggcgctc gaccagcacc
      721 cggtcgtctt cggcgaaggc cgcggccaac gcggaatcca actcgtctgc cgccgccacc
      781 cggccgacgc cgacgctcga gccgccgcgt gccggcttga cgaacgcagg caggccccac
      841 tccagagcct cggcgtgcac ctcgtgcggc gccgcccgcc acctggcccg gctgacgacg
      901 ctccacgggg cgacggcgat gcccgccgcg cgcagcacgg tcttggtgaa gtgtttgtcc
      961 atcccgaggg cgctgccgag cacgccggag ccgacgtacg gcatgtcgac gaggtcgagc
     1021 atgccctgga tcgtgccgtc ctcgccccag cgaccgtgca aaagaggaaa gacgacgtcg
     1081 acggcgacgc gggccgccgg cgcgtcgggc ctcgacaccg tgagaacacg ctcaccggcg
     1141 cgcgtcggcc aggcgacgcg gctgccattg tcggtgacgc ggggcggctc gcccgcagcg
     1201 ctcatgcgga aggcctccgg gtcgtcttgt tccaagacga agaccccgtc ggcggtgacg
     1261 cccacgggga ccacgtcgta acggtcacgg tcgatggccg cgagaatccc ggcggcggtc
     1321 gcgcaactta tcgaatgctc gctggaacgc cccccgaaga cgagcgcgac cgtcactgtg
     1381 cccatgcggg ttcctctcgc cttgtggctc gtcggattcg gtcgccatat gcggcgcgat
     1441 gtctttcggg tccagggtac cgtcgagcac ctgccccacc tgacgcacga tgggcatccg
     1501 cacgccgcgc tgccgggcga gcgccagcac cgccttcacc gaggtaaggc cctctgcggt
     1561 ctgatccatc tgcgcgatga cgtcggagta gctgtagccc tgcccgagaa gccgcccggc
     1621 cgtgttgttg cgcgagagcg gcgacgtgca ggtggcgatg aggtcgccaa ggcctgcgag
     1681 cccggtgagc gtctcgggcg cggccccgta ggccacggcg aagtccgtca tctcgacgag
     1741 gccgcgggtg atgatcgccg ccttcgtgtt ctcgccgtag ccgaccccgt cgacgatgcc
     1801 gatcgcgacg gcgatgaggt tcttcaggat gcccgcgaac tccgtgccga cgacgtcggt
     1861 gcccacaaag aacttcatgt agccgttgcc ggccgtgcgg gccacccgcc ccgccgtctc
     1921 caggtcttcg gaggcgacga cggaggcggt gggctgctcc cgggcgatct cgagcgccag
     1981 attcggcccg gaagcgacgg cgatgcggcc cggatccagc cggagctcct cggcgatcac
     2041 ctcgctcatc cggcgcccgg tcgtgcgctc gacccccttg atcaggctga cgacgatgct
     2101 cccgtcgtcg aggtggcccg agaccagccc gaggccccgg cgcaaggact ggctgggcac
     2161 cgacaggtag acctgctcgg cgccgtccaa cgcctgatcg agacgggtcg tggcgtgcac
     2221 ccggcgcggc aggttgacgc cgggcaggta ttcgctgttg cggtgcgcct cgttcagctc
     2281 ggtcgcgacc tcggcacgcc ttgcccacac cgtgacgtcg gcgccggcgt ccgcgagaac
     2341 cttcgcgaag gtcgtgcccc agctcccggc gccgaacacg gcgacgcgag cgggcctggc
     2401 ggcgtctcta cgcttcaaaa cgcccggtct ccttctggtc gtgcgccgcg ggatcccagc
     2461 gacctgtggg cggggtcgtg ccgcgcagcc tcgcgacgag gcctgtgatg gcgaccatga
     2521 tcgcctccgt cgcctccgcc agcgtcgtcg cgtcgatcgg ccggccccgg aacgcctcca
     2581 ggtcgaccgg gtctccgaag acgatgcgga tgcggcgggg cgggaagagg cgcacccggt
     2641 gcgagtagcg gccgagcacg tcttgcacgc cccagtgggc gacggggatg accggcaggc
     2701 cgtagcgcaa cgccatgcgg gccgcccccg tcttgcctcg catcggccag aggtcggggt
     2761 cgcgcgtgag cgtgccctcg gggtagacga taaccgcgtt gccacgctcg gcgacgagcc
     2821 gcgcggcgtc cagcggggag ccctgcctgc tcacggcagc ccgttcgacg gggatctggc
     2881 cgcttcgtcg caagagccat cccacgatcg gaacgcgaaa aagggatccc ttggcgagga
     2941 agcgcggaag ccggccgagc cgccacacgc cgacgccgac gatgacgggg tcgagctcgc
     3001 tcacgtggtt cggcacgatg atgcaggcgc cgtgcggcgg cagcttgtgg ccgtcgacga
     3061 tacgcagcct cgtcaccgtg ctgatcaccg gcaagatgac gagggccagg atccagaagg
     3121 cggagggccg ggcgcgctcg gtccgccgcc gacgcccggc ccgcgccggc gcgtcagcct
     3181 tcaaggtcga aatccgcgcc cagcatatgc agcttcgtga ggaagtcttc gtacccgcga
     3241 cggatgatgc cgacgttgct cacccgggag cgtccggtcg cgctcaacgc ggcgatgagg
     3301 tggctgaaac cgccgcgcag gtcgggaacg gtgatgtcgg cgccgtgcag ccggctcggt
     3361 cccgcgatga cggccgaatg atcgaagttg cgctgcccga aacggcacgg atgcccgccg
     3421 aggcactcct tgtgcacctg gatcgtggcc cccatcttga cgagcgcgtc gacgaagccg
     3481 aacctcttct cgtagacggt ctcgtgcacg atcgagaccc cgtcggcgcg ggtcagcgcg
     3541 acgacgagcg gctgctgcca gtcggtcatg aaaccggggt gcacgtcggt ctcgatgacg
     3601 acgggtttca gcctgccgcc cggatggtag aagcgaatgc cgtcgtcgcg gatgtcgaag
     3661 gcgccgccga ccttgcgata gacgttcaag aaggtgagca tctctgactg ccgggcgccc
     3721 gccacgaaga cgtcgccctc ggtcgcgagg gcggcggcgg cccagctggc ggcctcgttg
     3781 cggtcgaaga tggcggtgtg ctcgtagccg acgagccggt cgacgccctc gatccggatg
     3841 atccggtcgg tgtccaccgt gatgatcgcg cacatcttct gcaagatgtt gacgaggtcc
     3901 atgatctcgg gctcgatggc ggcaccccgc agctccgtga tgccgtcggc acgcacgccg
     3961 gtcaggagga cctgctccgt cgccccgacg ctcggatatg ccagctgcac cttcgtgccg
     4021 acgagcccgt tcggggcggt catgcggatg ccgctcggca gcttctcgac gaccgcaccg
     4081 aaactgcgca gcacgtccag gtggtaattg atcggccgat ccccgatgcg gcacccccca
     4141 aggtcgggaa tgaacgcctc gccgaggcga tgcaggagcg ggccgcagaa gaggatcggg
     4201 atgcggctgg agcccgcgta cgcgtcgata tcggcgatat gcgcgctctc caccgccgac
     4261 gggtcgagcg tcaaaacccc ttcggggcca gaactcacct gcaccccgtg cacctctaag
     4321 aggtcgcgca cgatgcggac atcgctgatg tcgggcacgt tcttcaggat gctcgggccg
     4381 tcaccgagca gggcggcgac catcgccttg gtgacgagat tcttggcgcc gtgcacgccg
     4441 atccgaccct cgagaggccg gccgccacgg atcgtgacga cttcgggcgc cgccagacgc
     4501 gccgcggagg tccggagggt ctgcgtggtt agtgtgttca ctctgctcca ccggcatgat
     4561 cgccgacggc gggcatgcac cctgccggct gttgagtaag gaggtccgac ccgcacggcc
     4621 cgttttcagg tcgcccgcgc cgcatccagt gtaatcggcg ggtgggtctc ggcctgcgaa
     4681 gacgccgcga gccgccgtgg atcagacggc gcccgcccgg cccggaacgt ggagggcgca
     4741 ggcgggcgcc gggatccggc ccgtcagttc tcgacgaacg cgggcaacgt gctcggccgc
     4801 cacgcggcgc gggtcttctc gtaggcggtg atctggtcgg cgtgctgcag ggtcagggcg
     4861 atgtcgtcca acccctccat gaggcgccag cgcatgtagt cgtcgatctg gaagtcgacg
     4921 gtgagcccgc ccaccgtcgc ggtgcgctcc acgaggtcga cccgcgcctc gacacccggc
     4981 cgggcctcga tggcctccca caaccgctcg atgtcctcct gcgccacctg ccccgtcacg
     5041 aggccctgct tgccggagtt accgcggaag atgtcggcga aacgagggct caagaccgcg
     5101 gtgaaaccgt agtctcgcag cgcccagacg gcgtgttccc ggctggaacc ggtgccgaag
     5161 ttcgggccgg ccacgagtat cttgcccgcc cgatacggct cctgattgag gatgaagtcg
     5221 gggtcttgac gccagcggaa gaacagcgcg tcgtcgtagc ccgacttggt gatcctcttc
     5281 aagaacttcg cggggatgat ctggtctgtg tcgacgtcgg agcgccggaa cggcacggcc
     5341 accccggtca agacggatac cttctccacg ctcacgcctc cgttccggcg ccgaccaaca
     5401 ggtcgtcgcg ttcgatgtcc cacgggctcg acagagtgcc gcgcacggcc gtcgccgccg
     5461 cgacgagcgg ggagaccaga tggctgcgcc cgcccttgcc ctgccggccc tcgaagttgc
     5521 gattcgaggt ggaggcgcac cgttcgccgg gctgcagctg gtcggggttc atgccgaggc
     5581 acatcgaaca gccggcggcg cgccattcgg cgccgaagtc ttcgaagagc gtgttcagcc
     5641 cctcggcctc ggcttgcagc cgcacccggg cggagccggg cacgacgagc acgcgcacgt
     5701 tcggcgcctt cttgtggcct cggatgatcg acgcgaagtc acgcaagtcc tcgatacggc
     5761 tgttggtgca ggagcccatg aacacgacgt cgaccggaat gcccttcatc ggcgtgcctg
     5821 gcttcaggtc catgtattcg agcgcccgct gcgccgagga gcgcaggttc gggtcgtcga
     5881 acgagtcggg gtcgggcacc gactccgaca tgggcacgcc ctgggcgggg ttcgtgcccc
     5941 aggtgacgaa cggctcgagc ttgtcggcgt cgatgtccac ttctttgtcg aagaccgcgt
     6001 ccgggtcgct tctcagcgtg tcccagtaag cgaccgcctc gtcccacaag gcacccttag
     6061 gcgcgtgggg gcg
//
LOCUS       DASTNL010000237        12064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_735667, whole genome shotgun sequence.
ACCESSION   DASTNL010000237 DASTNL010000000
VERSION     DASTNL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735667"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..513
                     /locus_tag="VFQ96_07660"
     CDS             133..513
                     /locus_tag="VFQ96_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001000 - bacterial-type RNA polymerase
                     core enzyme binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEU0257699.1"
                     /translation="MADRSLRGMRLGSQSLQSEEGVVYSPRTEYTYRCSQCGEDTVVV
                     FSAEAEAPHTWECKHCAHEARLLVGAKAVEVDHSKDKPVRTHWDMLMERRTRAELEEL
                     LEERLAYLRARRGKAAPKGRKVGA"
     gene            complement(617..2293)
                     /gene="lnt"
                     /locus_tag="VFQ96_07665"
     CDS             complement(617..2293)
                     /gene="lnt"
                     /locus_tag="VFQ96_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU0257700.1"
                     /translation="MVTTGFERTRNGEDAGLRRLPPPHPGGFDRVGKRAAARPRPLMP
                     LWMALVMAAAAGPAYSAAFPSPGLWPLVFPALAAMILPLVGRRAGTAALLGFLSGFGF
                     FLVHVPWLTYFLGHVAWYVADVPWVGLSFAEGVYFAAAAPLITLAFRWLPRVWPSRLG
                     RLGFLPVVVAGLWTAREILAGSWPYGGFAWGRAGLSQSQSPLAGLAAWIGISGVSFVL
                     VLLVVFVVQVFLEPDVRLTGRFVLSVAAGALVFAVPAWPVAVDGYTTVAAVQGNGKAA
                     YADHAPAGSVLVSQVNATIPVLDRNVDMVVWPEGGSDLDPMAVPAAAQVLDQISATMQ
                     APIVTGTITKSGGRYFNSSIVWQAGQGEVAQYDKRHPVPFGEYIPDRAFWHAVAPGFV
                     DLLTRQYTPGTRPNVMPVGNIEAGVAICFDIADDGLFADMMQGGAQIILAQTNNADFG
                     HSDENEQQLAIARMRAIEGGRSLVNISTVGRSQIIGPDGRTLDEIPAYKPGALVDAVP
                     LATETTPASIASVEVGFFVSGLGLAGVVAAGFFVAAGRVSRRRAARRAAR"
     gene            complement(2287..4719)
                     /locus_tag="VFQ96_07670"
     CDS             complement(2287..4719)
                     /locus_tag="VFQ96_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0257701.1"
                     /translation="MTGEPSPAQRYAAARSRTRHPDVARFEERLPFDLDPYQRRACTA
                     VSEDRSVLVAAPTGAGKTVVAEFAVFLAMQTARQKVFYTTPMKALSNQKYQDLCAGYG
                     ADRVGLLTGDSSVNPGARVVVMTTEVLRNMLYASSPLLDGLAWVVLDEVHYLGDRFRG
                     AVWEEVIIQLPAEVRTVSLSATVSNAEEFGAWLQAVRGDTAVIVSERRPVPLTHHVAV
                     GSRLTGLFEGGSATGVVRRVNPELLRLVRARGLRAVPGRRSGGYRGAGARRAAHTGYA
                     TMVRLLGDEGLLPSIVFIFSRAGCDRAVEQLVRAEVTLTSEAERREIRESVEARCRTL
                     LDEDLGALGYWDWLKALQRGIAAHHAGLLPAFKETVEDLFRRKLVKVVFATETLALGI
                     NMPARSVVLDSLEKFNGESTVPVTPGEYTQLTGRAGRRGIDDEGHSVIRWRPGLDAQA
                     VASLASRRSYPLNSSFRPTYNMAVNLVERFGRERTREVLESSFAQFQADRTVRELEET
                     IRQHETALEGYAEAMRCDLGDFTEYAALRRSLSDVERRGRGTAASSAPDRARIVELRQ
                     ELRRHPCDRCPDRETHARWAARWWRLERQTAGLREQVARRSGRIATLFDRVSDVLVDC
                     GYLASVADAQAGSGRTLVATVHGRMLGRIYGERDLLVAECLRRNVWRELDAAALAAVA
                     AALTYEPRHAQAEPDPPPLRGSLGRALEKTRQVWERLDDVERAHLLPGTPPIPMGLCP
                     AMRDWARGVALRQVLRQAGLAAGDFVRAAKQAIDLLGQVRLVAPGPVARTASAAIDAL
                     AHGVVAYSSVPW"
     gene            complement(4751..5500)
                     /gene="tatC"
                     /locus_tag="VFQ96_07675"
     CDS             complement(4751..5500)
                     /gene="tatC"
                     /locus_tag="VFQ96_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU0257702.1"
                     /translation="MSLGDHLRELRKRLFRAAAGLIAGAVGGWFLVPYLLDAMRAPVL
                     EIAHRHELASLNFPNITSAFDLKLEMAVTIGAVISSPIWLYQIFAFLVPGLNRREKKY
                     VFGFFFSAVPLFFAGCFAGWYVLPHIVGLLTSFAGRQDSTLIDAPTYYGFVMKLVIIV
                     GVAFVLPVFMVLLNFMGILSAAVLRRSWRVAMIIIVLFTATATPSVDVVSMFLLAAPM
                     AVLYFAATGISMIHDRRAAKLRAKLSAELTP"
     gene            complement(5554..5802)
                     /locus_tag="VFQ96_07680"
     CDS             complement(5554..5802)
                     /locus_tag="VFQ96_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEU0257703.1"
                     /translation="MFGNTFTGWHLVIILAIVLLLFGAPKLPALAKSVGQSMRIFRGE
                     MKNLQKDEESERSSTSQDAGTTHREKDSGDEKPGSSAE"
     gene            complement(5907..7964)
                     /locus_tag="VFQ96_07685"
     CDS             complement(5907..7964)
                     /locus_tag="VFQ96_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEU0257704.1"
                     /translation="MSAHASRVPVEERLFSVVLALVATENGLTRDEILRSVQGYRQRF
                     EEGGAGPSLERQFERDKEDLRDLGIPLEVLEEPGRAGDNHGLRYRIPKKRYDMPSDVV
                     FTAEERALLALAAAVWRQGSLSAQSRRALTKLRGVGAEPVEPLIGLAPRLNAREASFE
                     PLSRALERHLAVRFSYLKPGDAGPRARVVEPHALVQHRGRWHLYGWDRGADAVRTFLL
                     TRITGQVTLTSDVFAPPGGDPAGQALRELDLLWERQSARLLVAEGSEAALQLSRRVSP
                     APAGPGGGPAGHGSVLAAHYTDLHVFADELAGYGPEVVVAAPRKLREAVIGRLRSVAA
                     AHAKEPPAAPERLAPVRPPAAASAGHATDRVVLLLALIPYVLEHGSVTVPEAAQHFGV
                     AAERIRRAVRLIAVSGVPGQSRRYLPGDLFDIDWDDFEDHDRIVLTHLVAIDEVPRLS
                     AREAAALIAGLQYLQPWLAGEQRAGEASLMAKLVRSSSAEPSAVAVDAGAPDDRIGPL
                     RAAIEQARQVEFDYHSVGGETMRRIVDPLRLDAIDDAWYLRGWCHTRQAVRTFRLERV
                     SGLRATARPTVFRAGSLPIPERIFDGTGADFVIDVEVDDGYLPMIVAYLEADTPVVRR
                     GEAWRTTVRAAHVHGVKRLAARLCGRLRVIEPASARRAVAEWAREGLAGYAVPAPPRP
                     QSP"
     gene            complement(8018..8974)
                     /locus_tag="VFQ96_07690"
     CDS             complement(8018..8974)
                     /locus_tag="VFQ96_07690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU0257705.1"
                     /translation="MRRAATIVALAGLVAAGLAGCSSTASAANCGSAAAPGAASDTIH
                     VAGPFGTSSPEVSFPTPLKTTTTQRTILTPGSGPLLQDGQTVVGEISIYNGATGQLMP
                     PASYGAAPGPITFNIGGTLPGISNGLLCARDHSRVAIAVSPNDGTKDGATSLVVVVDV
                     KKAYLPRANGTPDVHVPNTPRVFLAPNGAPGIVVPNVAPPKKLTVIPTKTGHGESVKK
                     GDAVIVQFTGVVWNDDKNVFTSTWNGSGATAIVADSSSSGPLADGVTRGLAQALIGAK
                     VGSQLQVTVPPRLGYGASGSAEAPKNATLVYVVDVLGIHHSS"
     gene            complement(9070..10080)
                     /locus_tag="VFQ96_07695"
     CDS             complement(9070..10080)
                     /locus_tag="VFQ96_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEU0257706.1"
                     /translation="MTPSDGVFLPGERVQLTGPKGKHHTIVLVAGGQFHTRKGVVHHD
                     DVLGRPDGITVTTSMGVDYLALRPLLHDFVMSMPRVTALVYPKDAAQILAVADIHPGA
                     RVVEAGAGSGALSLWLLRAVGAEGHLFTFERRAEFADVARANVASYYGEAPRAWSLTL
                     GDLAQEGPDVLGEGSADRAVLDMLAPWECLPVITRLLRPGGVLTCYVATVTQLSRLAE
                     AIRQVGRFTEPESTETIQRGWHVDGLAVRPDHRMIGHTGFLLTTRILAPGTVPPRPGR
                     LASKTAYGPEDVEAWTPGALGQQQVSARSIRKRARAAADAADKARHPSSLPDSPEPPG
                     AG"
     gene            complement(10077..10775)
                     /locus_tag="VFQ96_07700"
     CDS             complement(10077..10775)
                     /locus_tag="VFQ96_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU0257707.1"
                     /translation="MTPATAPSDHPGPLPQAILWDMDGTLVDTEPFWMNAERELTDRY
                     GATWTEEDVLRVVGAGLGTTAGHLRERGVPLEPDEIIDRMTSSVIQALAEGEIPWRPG
                     ALELLREARAAGVSQALVTMSMAPMAHAIVHRMPFTVFDTVVTGDAVSRPKPHPDAYL
                     AAAAALGVDAASSVAIEDSVPGVAAAVAAGAVTIAVPHVTQVPESADHETWPSLRGRT
                     LGDLGEVMRRRGRR"
     gene            10889..11776
                     /locus_tag="VFQ96_07705"
     CDS             10889..11776
                     /locus_tag="VFQ96_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEU0257708.1"
                     /translation="MEDPTRTPAGRVLVVAFDGWNDAGDAASTAVRILRDSRDVVPVA
                     QVDPETYFDFQFARPTVSLDEDGRSSIHWPEVVLSAPAPTAALPHDAPEVCILSGTEP
                     SRNWKAFTAEIVDAALTAGVEWVILLGAMLADVPHTRPISVFASSSDARVRARFGLER
                     STYEGPTGILGVLADATERAGLPTVSVWASVPHYVHNAPSPKATLALIGRLEELIGVS
                     IPHGNLAEQSAAWEAGIDALAADDEDMAAYIKQLEQARDTVESPDASGDAIAQEFERY
                     LRRRGDDGRGDEPWHGGRD"
     gene            complement(11816..>12064)
                     /locus_tag="VFQ96_07710"
     CDS             complement(11816..>12064)
                     /locus_tag="VFQ96_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEU0257709.1"
                     /translation="AETDLTGAEARIATWRAGLARRHDAASAEPAAGVLAQVRRALAD
                     DLDTPAALAVVDAAADSGTDDPELVASLTDALLGVRLA"
BASE COUNT         1963 a         4183 c         4214 g         1704 t
ORIGIN      
        1 ggcgcgtctc gggcgcctgg ccggacagag cgcgaatact cccggcgacc ggtttgttga
       61 tgtcggtggt gaggcaatct caagcttggc tcgatataaa tagaggttgc atcgcgataa
      121 ggagaccaca caatggcaga tcgcagtttg cgcggcatgc gactcggatc ccagagcctg
      181 cagagcgaag agggcgtcgt ctactcgccc cgcaccgaat acacctatcg gtgttcacag
      241 tgcggcgagg acacggtggt cgtcttctcc gcagaggccg aggcgccgca cacctgggaa
      301 tgcaagcact gcgcgcacga ggcccggctg ctggtcggcg ccaaggcggt ggaggtcgat
      361 cactcgaaag acaagccggt gcgcacgcac tgggacatgc tgatggaacg ccgcacgcgg
      421 gcggagttgg aggagctgct cgaagagcgc cttgcctatc tgcgcgcccg gcgcggcaag
      481 gcggcgccga aggggcgcaa ggtcggcgcc tgaggcgccg cgcggccacg tctcccgacg
      541 cctctcgcag cgggcacggt gaaggtccgg cacgccgcct gatgagccgc gacggcactg
      601 agcggggcgt gggccgtcag cgtgcggcac ggcgagcggc gcgccgtcgg ctgacgcgcc
      661 cggcggcgac gaagaacccg gccgcgacaa ccccggcgag gcccaggcct gagacgaaga
      721 acccgacctc gacgcttgcg atcgaggctg gagtcgtctc cgtcgcaagc ggcaccgcgt
      781 cgaccagggc gcccggcttg taggcgggaa tctcgtcgag cgtgcggccg tccggaccga
      841 tgatctggct gcggccgacg gtggagatat tgacgaggga acgcccgccc tcgatcgcac
      901 gcatgcgggc gatggccagt tgctgctcgt tctcgtcgct gtggccgaag tcggcgttgt
      961 tcgtctgcgc aaggatgatc tgggcgccgc cctgcatcat gtcggcgaag aggccgtcgt
     1021 ctgcgatgtc gaagcatatc gcgaccccgg cctcgatgtt cccgaccggc atgacgttcg
     1081 ggcgggtgcc cggcgtgtac tggcgggtga gcaggtcgac gaagcctggc gccacggcgt
     1141 gccagaatgc ccgatcggga atgtactcgc cgaacggcac cgggtgtcgc ttgtcgtatt
     1201 gggccacctc gccctgcccc gcctgccaga cgatcgacga gttgaagtag cggccgccgc
     1261 tcttggtgat cgtgcccgtg acgatcgggg cttgcatggt ggcgctgatc tggtcgagga
     1321 cctgcgcggc cgcgggcacg gccatcgggt cgaggtcgct tccgccctcg ggccagacga
     1381 ccatgtcaac gttgcgatcc aggacgggaa tcgtcgcgtt gacctgactg accagcacgc
     1441 tgccggccgg cgcgtgatcg gcgtaggcgg ccttgccgtt gccttggacg gcggcgacgg
     1501 tcgtgtagcc gtcgaccgcg acgggccagg ccggcacggc gaagacgagc gctcccgcgg
     1561 cgaccgagag cacgaagcgg ccggtcaggc gcacgtcagg ctcgaggaag acctgcacga
     1621 cgaagacgac gaggagaacg agcacgaagc tgacgcctga gatgccgatc cacgcggcga
     1681 gccccgcgag ggggctttgc gactgcgaca gcccggccct gccccaggcg aagccgccgt
     1741 acggccacga cccggcgaga atctcacggg ccgtccacag gcccgcgacg acgacgggca
     1801 agaaacccag cctgccaagt ctcgagggcc acacccgcgg cagccaacgg aaggcgagcg
     1861 ttatgagcgg cgcggccgcc gcgaagtaca ccccttccgc gaacgacagg ccgacccacg
     1921 gcacgtcggc cacgtaccag gcgacgtgcc ctaagaaata ggtgagccac ggcacgtgca
     1981 cgaggaagaa gccgaacccg gagaggaagc ccagaagcgc ggccgtgccg gcccggcgcc
     2041 cgacgagcgg gaggatcatc gccgccagtg ccgggaagac gagcggccag aggccgggcg
     2101 acgggaaggc cgcgctgtac gcggggccgg ccgcggccgc catgacgaga gccatccaca
     2161 acggcatcaa cggccgaggc ctcgcggcag ccctcttgcc cacacggtcg aacccgcccg
     2221 gatggggcgg cggcaggcgc cgcaggccgg cgtcttcccc gttccgcgtg cgctcaaaac
     2281 cggtcgtcac cacggcaccg aactgtacgc cacgacccca tgagcgagag catcgatcgc
     2341 ggcggaggcg gtgcgcgcga ccggcccggg cgcgaccagc cgcacctgcc ccaagagatc
     2401 gatcgcctgc ttggcggcac ggacgaagtc gccggccgca agcccggctt ggcgcaacac
     2461 ctggcgcaat gccactccgc gagcccaatc ccgcatcgcc gggcacaggc ccatggggat
     2521 cggcggggtg ccgggcagga ggtgtgcgcg ctccacgtca tcgagccgct cccacacttg
     2581 ccgggtcttc tccagcgccc gtcccaggga gcctctcagc gggggcgggt cgggctcggc
     2641 ctgggcgtgc cgcggctcgt atgtcagcgc ggccgccacg gcggccaagg ccgccgcgtc
     2701 gagttctcgc cacacgttgc ggcgcaggca ttcggcgacc agcaggtctc gctccccgta
     2761 gatgcggccg agcatcctgc cgtgcaccgt ggccacgagc gtgcgcccgc tgcccgcctg
     2821 cgcgtcggcg acggaagcaa gatagccgca gtctacgagc acgtcgctca cacggtcgaa
     2881 cagcgtggcg atgcgcccgc tgcgcctggc cacctgctcc cgaagccctg ccgtctgtcg
     2941 ttcgagccgc caccacctgg ccgcccagcg ggcatgcgtc tcgcggtcgg ggcaacggtc
     3001 acacggatgg cgtcgcagct cttggcgcag ctcgacgatt ctcgcgcggt ccggggcgga
     3061 tgaggcggcc gtgccacgcc cccgccgctc cacgtcggac aacgaccggc gcaacgcggc
     3121 gtactcggtg aagtcgccga gatcgcagcg catcgcctct gcatagccct ccaacgccgt
     3181 ctcgtgctga cgaatcgtct cctccaactc acgcacggtg cggtcggcct gaaactgggc
     3241 gaacgacgac tccaagacct cgcgcgtgcg ttcgcggccg aaacgctcga cgaggttgac
     3301 cgccatgttg taggtcggcc gaaaactcga gttgagggga tagctgcgcc tcgacgccag
     3361 cgacgcgacg gcctgcgcat cgaggcccgg cctccagcgg atgacgctgt ggccttcgtc
     3421 gtcgatgccg cgccgcccgg cacggccggt cagctgggtg tactcccccg gcgtgacggg
     3481 caccgtgctc tcgccgttga acttctcgag gctgtcgagg acgacgctgc gggccggcat
     3541 gttgatgccg agggcaagcg tctccgtcgc gaagaccacc ttcacaagct tgcggcgaaa
     3601 gaggtcttcg acggtctctt tgaatgccgg cagaagaccc gcgtgatgtg cggcgatgcc
     3661 gcgttgcagc gccttcagcc agtcccaata gcccaacgcg ccgaggtcct cgtcgagcag
     3721 ggttcggcac ctggcctcga cgctctcccg tatctcgcgc cgctccgcct cggaggtgag
     3781 cgtcacctcc gcgcgcacca actgctcgac ggcacggtcg catcccgccc ggctgaagat
     3841 gaagacgatc gagggcagaa ggccttcgtc gcccaagagt cgcaccatcg tggcatagcc
     3901 ggtgtgcgcg gcacgtcggg cgcccgcgcc gcgatatccg ccactgcggc ggccagggac
     3961 cgcccgaaga ccccgggcac gcaccagccg aagaagctcc gggttgacgc ggcgcaccac
     4021 gcctgtggcg ctgccaccct cgaagagccc cgtcaagcga gagccgacgg cgacgtgatg
     4081 ggtgaggggc accgggcgcc gctcggaaac gatgacggcg gtgtcgccgc gcaccgcctg
     4141 cagccaggcg ccgaactctt ctgcattcga cacggtcgcg ctcaacgaga ccgtgcgcac
     4201 ctcggcgggg agttggatga tcacctcttc ccacaccgcg ccgcggaacc ggtcgccgag
     4261 gtaatgcacc tcgtcgagaa cgacccaggc caggccgtcg agaagaggcg aggaggcgta
     4321 cagcatgttg cgtaagacct cggtcgtcat gacgacgacg cgggcacccg gattgacgct
     4381 gctgtccccc gtgagcagcc cgacacgatc ggcgccgtag ccggcacaca ggtcctggta
     4441 tttctggttg ctgagcgcct tcatcggcgt cgtgtagaac accttctgcc gcgccgtctg
     4501 catcgcgagg aagacggcga actctgccac gaccgtcttg cccgcgccgg tcggcgcggc
     4561 gaccagcacg ctgcgatcct ccgagacggc cgtgcaggca cgacgctgat acgggtcgag
     4621 gtcgaacgga aggcgctcct caaaacgcgc gacatccggg tggcgggtgc ggctccgggc
     4681 ggcggcgtaa cgctgcgcgg gtgaaggctc gccggtcaca ctcccactct aggtggttcc
     4741 cgcgacccgc ctacggggtc agctccgcgc tgagcttggc ccggagtttg gccgctcgac
     4801 ggtcgtggat catcgagatg ccggtcgccg cgaaatacag caccgccatc ggcgccgcca
     4861 ggaggaacat cgagaccacg tcgaccgagg gggtggccgt cgccgtgaag agcacgatga
     4921 tgatcatcgc cacccgccag gagcggcgca gcacggccgc gctcaagatg cccatgaagt
     4981 tcaacagcac catgaagacc ggcagcacga aagccacgcc gacgatgatg acgagtttca
     5041 tgacgaaccc gtaatacgtc ggtgcatcga tgagcgtcga gtcttgccgg cccgcgaaac
     5101 tggtgagcag gccgacgatg tgcggcaaca cataccagcc cgcaaaacag ccggcgaaga
     5161 aaagcggcac ggcgctgaag aagaacccga acacgtactt cttctcgcgc cggttgaggc
     5221 cgggaacgag gaacgcaaag atctggtaca gccagatcgg gctggatatc acggcgccga
     5281 tcgtgaccgc catctcgagc ttcaggtcga aagcgctcgt gatgttggga aaattcaggc
     5341 tcgctaactc gtgacggtga gcgatctcta aaacgggcgc acgcatggcg tccaagaggt
     5401 acggcacgag gaaccatccc ccgaccgctc cggcgataag gccggcggcg gcacggaaca
     5461 gccttttgcg taactcccgt aagtgatcgc cgagcgacat ccgccgttcg tggtcaccct
     5521 cgtgttgacg gtgatgtcga gccactgccg gggttactcc gccgaggagc ctggcttctc
     5581 gtcgccggag tccttctcac gatgcgtcgt gccggcgtct tgagacgtcg aggaacgctc
     5641 cgactcctcg tctttttgaa gattcttcat ttcgccgcgg aagatccgca tcgactggcc
     5701 gacgctcttc gccaaggcag gcagcttcgg ggcaccgaag agcaaaagca cgatggccaa
     5761 gatgatgacg agatgccatc ccgtgaatgt gttaccgaac aatggagcca tccttgtggt
     5821 gtgtggcgtc gtcgcagaca accgtggtta cgcgaagtct atcctccgca gccttgcaag
     5881 caccgcgatg atccgtctcg tttgcgtcac ggcgattgcg ggcgaggagg cgccggcacg
     5941 gcatagcctg cgaggccttc ccgggcccat tcggcgaccg cccgacgcgc cgaggctggc
     6001 tcgatcacac gcagccggcc gcaaagcctg gccgccaggc gtttgacgcc gtgcacgtgt
     6061 gcggcacgaa ccgtggtgcg ccaggcctct ccgcgacgca cgacgggcgt gtcggcctcc
     6121 aggtaggcca caatcatcgg caaatagccg tcatccacct cgacgtcgat cacgaaatcc
     6181 gccccggtgc cgtcgaaaat acgctccgga atgggcaagc tgccggcacg gaacacggtc
     6241 ggccgggccg tcgcccgcaa gccggagacc cgctcgaggc ggaaggtacg cacggcctgg
     6301 cgggtgtggc accagccgcg caggtaccag gcgtcgtcga tcgcatcgag ccgcagcggg
     6361 tcgacgatgc gcctcatcgt ctcgccgccc acgctgtggt aatcgaactc cacctgcctc
     6421 gcctgctcga tcgccgcacg caagggcccg atccggtcgt cgggcgcgcc cgcgtcgacc
     6481 gcgaccgcac tcggctcggc cgacgagctg cgcaccagct tcgccatgag gctcgcctcg
     6541 ccggcgcgtt gctcgccggc cagccaaggt tgcaggtact gcagcccggc gatgagcgcc
     6601 gccgcctcac gcgccgagag acgaggcacc tcgtcgattg cgacgaggtg ggtgagaacg
     6661 atccggtcgt gatcttcgaa gtcgtcccag tcgatgtcga agaggtcgcc cggcaagtac
     6721 cggcgcgact gccccggcac gccggagacc gcgatgagcc gtacggcccg acggatgcgc
     6781 tccgccgcga cgccgaagtg ttgtgcggcc tccggcaccg tgacggaacc gtgttccaag
     6841 acgtacggga tcaaggcgag gaggagaacc acgcggtcgg tcgcatgacc ggccgacgcg
     6901 gccgcggggg gccgaaccgg cgccaggcgc tcgggcgcgg ccgggggctc cttcgcatga
     6961 gcggcggcaa cgcttcgaag acgcccgatg acggcctccc gaagcttccg aggcgccgcg
     7021 acgacgacct ccgggccgta ccccgccagc tcgtcggcga aaacatgcag gtcggtgtag
     7081 tgcgcggcaa ggacggatcc gtgcccggcc gggccgccgc ccgggcctgc cggggccggg
     7141 gagacgcgac gagagagctg cagcgcggcc tcgctgccct ccgcgacgag gagccgtgcg
     7201 ctttgtctct cccacaaaag gtctagttcg cgaagcgcct gaccggccgg gtcgccaccc
     7261 ggcggggcga aaacgtcgga ggtgagcgtc acctgcccgg tgatgcgggt gagaaggaac
     7321 gtgcggaccg cgtcggcccc gcgatcccac ccgtaaaggt gccagcgccc gcggtgctgc
     7381 accaaggcgt gcggttcgac gacacgggcg cgcggcccgg cgtctcccgg tttcaggtag
     7441 gagaagcgca ccgcgaggtg acgctcgagg gcgcggctga gcggctcgaa cgacgcctcg
     7501 cgggcgttca gccgcggggc caggccgata agcggttcga cgggctccgc accgacgcca
     7561 cgcagtttcg tgagggcccg acgagactgt gcggagagcg agccctggcg ccagaccgcc
     7621 gcggccaagg cgagcaaggc tctctcttcg gccgtgaaga ccacgtcgga aggcatgtcg
     7681 taacgcttct tcgggatccg ataccgcagc ccgtggttgt cgcccgccct cccgggctct
     7741 tccagcacct ccagcgggat gccgaggtcg cgcaggtctt ctttgtcacg ctcgaactgc
     7801 cgctcgagac tcggccccgc cccgccctct tcgaaccgct gccggtaacc ctgcacgctt
     7861 cggaggattt cgtctcgcgt gagaccgttc tcggtcgcca ccaacgccag gacgacgctg
     7921 aagaggcgtt cctcgacggg gacacgcgag gcgtgtgcgg acacgcacat atcctaggcg
     7981 gcgccggcgc cccacccgtt cgagcgcgtg gcgggcgtca cgacgagtgg tggattccca
     8041 agacgtcgac gacgtagacg agggtggcgt tcttcggcgc ttcggccgag ccggaggcgc
     8101 cgtacccgag acgcggcggc accgtgacct gcagctgcga accgaccttc gccccgatca
     8161 atgcctgcgc gaggccgcgg gtgacgccgt cggcgagcgg gccggaggag gaggagtcgg
     8221 cgacgatggc ggtcgccccg ctgccgttcc acgtggaggt gaacacgttc ttgtcgtcgt
     8281 tccatacgac gccggtgaat tgcacgatga cggcgtcgcc cttcttcacg ctctcgccgt
     8341 gccccgtctt cgtcggtatc acggtgagct tcttcggggg cgccacgttc ggcacgacga
     8401 tgcccggtgc cccgttcggc gccaggaaaa cgcgcggcgt gttcggcacg tgcacgtcgg
     8461 gggtgccgtt cgcccgcggc aggtaggcct tcttcacgtc gaccaccacg acgagggagg
     8521 tcgcgccgtc tttcgtgccg tcgttcggcg agaccgcgat ggcgacacgc gaatggtctc
     8581 gggcacagag caaaccgttc gaaatgcccg ggagggtgcc gccgatgttg aaggttatgg
     8641 ggcccggtgc ggcaccgtat gacgcgggcg gcatcagctg cccggtcgcg ccgttgtaga
     8701 tgctgatctc gcccaccacc gtctggccgt cttgcagaag cggcccgctg ccgggggtga
     8761 ggatcgtgcg ttgcgtggtc gtggtcttca acggcgtcgg gaagctgacc tctggcgacg
     8821 aggtgccgaa cgggcctgcg acgtgaatcg tgtcggaggc cgcgccgggc gccgcggccg
     8881 aaccgcagtt cgcggccgac gcggtcgacg agcatccagc caggcccgcc gccacgagtc
     8941 cggcaagggc gacgatggta gcggctctgc gcacggctcc tctttcgttg actgtcgttt
     9001 cgttgactgc ctggcacgac ccggggtggc gccgcccggc atcccatgct agacgatgcg
     9061 cctaggcgac tacccggccc cgggaggctc cggggagtct ggcaaggaag acgggtggcg
     9121 cgccttgtcc gccgcgtcgg ccgccgcgcg ggccctctta cggatactcc tcgcgctcac
     9181 ctgctgctgc cccagcgcgc ccggcgtcca cgcctcgacg tcctccggac cataggcggt
     9241 cttcgaggcc agcctgccag gcctcggcgg cacggtgcca ggggccagga tgcgggtcgt
     9301 caagaggaac ccggtgtggc cgatcatgcg gtggtcggga cgcaccgcca ggccgtcgac
     9361 gtgccagccg cgctggatcg tctccgtcga ctccggctcc gtgaagcggc cgacctgccg
     9421 gatcgcctcg gccagcctgg agagctgtgt gacggtggcg acatagcagg tgagaacgcc
     9481 gcccggccgc aggagccggg tgatgaccgg caggcactcc catggcgcga gcatgtcgag
     9541 cacggcccgg tctgcactgc cttcgccgag cacgtcgggg ccctcttgag cgagatcgcc
     9601 gagagtcaga ctccaggccc gcggggcctc gccatagtag ctggcgacgt tcgcgcgggc
     9661 gacgtcggcg aactccgccc ggcgctcgaa cgtgaagaga tgcccctcgg cacccaccgc
     9721 gcgcagcagc cacagcgaga gggcgcccga cccggcgccc gcctcgacga cccgcgcacc
     9781 gggatggatg tcggcgacgg cgaggatctg cgccgcgtct ttcgggtaga cgagcgccgt
     9841 gacgcgcggc atcgacatga caaagtcgtg cagcaggggt cgaagcgcca ggtagtcgac
     9901 acccatcgag gtcgtgacgg tgatcccgtc gggccggccg aggacatcgt cgtggtgcac
     9961 gacgcccttg cgggtgtgaa actgcccgcc ggcgacgaga acgatcgtgt ggtgcttgcc
    10021 tttcggcccg gtcagctgca cccgctcccc cggcaggaag acgccgtcgg agggggtcac
    10081 cggcggcctc ggcggcgcat gacctcgcca aggtcgccga gcgtgcggcc ccggaggctc
    10141 ggccaggtct cgtggtcggc ggactcgggc acctgcgtga cgtgcggcac cgcgatcgtc
    10201 accgcacccg cggcgactgc ggccgcgacg ccgggaacgg aatcctcgat cgcgacgctg
    10261 ctcgcggcgt cgacgccgag ggccgcagcg gccgccaggt aggcgtccgg atgcggcttg
    10321 ggcctcgaca cggcgtcacc cgtcacgacg gtgtcgaaga cggtgaaggg catccggtgc
    10381 acgatggcgt gcgccatcgg cgccatcgac atcgtgacga gcgcctggct gacgccggcg
    10441 gcacgcgcct cgcgcaagag ctcgagcgca ccgggccgcc acgggatctc gccctccgca
    10501 agcgcctgga tcaccgacga ggtcattcgg tcgatgatct cgtcgggctc gagcggcacc
    10561 ccacgctctc gcaggtggcc ggccgtcgtg ccgaggccgg cgccgaccac gcggagcaca
    10621 tcctcctcgg tccaggtggc cccataccgg tcggtcagct cgcgttccgc gttcatccag
    10681 aacggctcgg tgtcgacgag ggtgccatcc atgtcccaca ggattgcctg cggcagcgga
    10741 cccggatggt cggagggtgc ggtcgcgggc gtcacaactc cccagtctat ggcccggcgc
    10801 ccggccgccc gctggccgac accactcggc cgtattcttg aggcgtggct aagacacgcc
    10861 acttcccgcg gacggaggca ggcgcatagt ggaagacccg acccggaccc cggcaggccg
    10921 cgtgctcgtc gtcgccttcg acggctggaa cgacgcgggc gatgcggcgt cgaccgcggt
    10981 gcggatcctc cgcgactccc gtgacgtcgt cccggtggca caggtcgacc cggaaacgta
    11041 tttcgacttc caattcgcga ggcccaccgt ctctctcgat gaagacggcc gcagcagcat
    11101 ccactggccc gaggtggtgc tctcggcgcc ggcacccacc gccgcactgc cgcacgacgc
    11161 gccggaggtg tgcatcctct ccggcaccga gccctcccgc aactggaagg cgttcaccgc
    11221 ggagatcgtc gacgcggcgc tcacggccgg cgtcgaatgg gtcatcttgc tcggcgcgat
    11281 gctcgctgac gtgccgcaca cccggccaat ctcggttttc gcctccagtt ccgacgcccg
    11341 ggtgcgggca cggttcggcc tggaacgcag cacctacgag ggccccaccg ggatcctcgg
    11401 ggtcttggcc gacgccaccg agcgtgccgg cctgccgacc gtctcggtgt gggcctcggt
    11461 gccgcactac gtgcataacg cgccgagccc gaaggcgaca ctggcgctca tcggccgact
    11521 ggaggagctc atcggggtgt cgattccgca cggcaacctg gcggaacaat cggccgcatg
    11581 ggaggccggg atcgacgcgc ttgcggccga cgacgaggac atggccgcct acatcaagca
    11641 gctggagcag gcacgcgaca ccgtcgaatc gccggacgcc agcggcgacg ccatcgccca
    11701 agagttcgag cgctacctgc gccggcgagg cgacgacggc cgaggcgacg agccctggca
    11761 cggcggccgc gactgaccac accggacacc gcgcgaaggc ctgggctaga cggactcagg
    11821 cgagccggac gcccaggagc gcgtcggtga ggcttgccac aagttccggg tcgtcggtgc
    11881 cggaatcggc cgcggcatcc acgacggcga gcgccgccgg ggtatcgagg tcgtcggcga
    11941 gcgcccggcg cacctgggcg agcacccctg cggcgggctc ggcgctcgcc gcgtcgtgtc
    12001 ggcgagcgag gccggcgcgc caggtggcga tcctcgcctc ggcaccggtc aggtctgtct
    12061 cggc
//
LOCUS       DASTNL010000238         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_1290689, whole genome shotgun sequence.
ACCESSION   DASTNL010000238 DASTNL010000000
VERSION     DASTNL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290689"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..804
                     /locus_tag="VFQ96_07715"
     CDS             4..804
                     /locus_tag="VFQ96_07715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0257710.1"
                     /translation="MLTIVARLTAPVPLAAQSTALNASQSSVGWIDFSDQFLGIVFDL
                     FPVALVIFLLWQHAPSRHGGERTLAAVSGTATSMTTTSSRAVGTAPTDQGAPTPGQSG
                     FRRIGLDLRRPGHDLALGVLLVAVIGIPGLGLYAAGRALGITVAVDAAPTHWLWWTVP
                     ILVFSAIRSGLQEEVIMVGYLFTRLPRVGWGPWRIILASALVRGSYHLYQGFGPFLGN
                     AVMGVVFGWYYHRHGRTMPLVVAHTLLDVISFVGYPLALAWWPALFTT"
     gene            complement(1071..1901)
                     /locus_tag="VFQ96_07720"
     CDS             complement(1071..1901)
                     /locus_tag="VFQ96_07720"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEU0257711.1"
                     /translation="MAEETSFSFIPRAYRPPKPRSYGLTEIRGPYYSTYGTRHLTDVL
                     EVAGQWVDGVKWAGGSFALLPREEVRKFSDIAHEHDAYISSGGWIETVLRYGPDAVDH
                     YLKEAKEVGFDVIEISAGFIALPTSGLMRLIELVKKAGLKPKPELGIQFGSGGDSGEE
                     ELAAEGHKDVAELIARANKALEAGAEIIMIESEGITENVVEWNTAPVGQIINGVGIEH
                     VMFESADPPVYEWYIKNYGNEINLFVDHSQILQLEGLRQGIWGNKSTWGRIINPAPRD
                     "
     gene            complement(1996..3369)
                     /locus_tag="VFQ96_07725"
     CDS             complement(1996..3369)
                     /locus_tag="VFQ96_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU0257712.1"
                     /translation="MTEVQDLARFVVGTKLADMSAKALDQLKIRVLDTLGVAIGALDA
                     TPTVAIRGLTEELGGNGQATLIGGGTSAPDRAAFYNTSLSRYLDFMDAFLSPGETNHP
                     SDNIGACLAASESVDAPGSEFLTAAAVAYQVQTRLSEVAPVRAHGFDHTVQGAYAVAA
                     AVSRALGLDAAKAANAIAISGTANNALRVTRTGDLSNWKGLAYPQVAKEGTFAALLAR
                     AGITGPSQVFEGNKGFKQTIAGDFSLDWSKENLEGVLRTIIKKHNAEIHSQSAIDAAL
                     ALREKPGFDPAKIRAVRIRIFQVAYDIIGGGEEGSKREIRTKEEADHSLLYMVAAALL
                     DGAVQPAQFEPKRILASDVQSLLARTKAQPDAGFSARFPKEMPVEVEVESEDGSVVAE
                     SRDGYRGFVNDPVDWDDAQAKFETLATDFAGPALRDEIVECVRGLERRQVRDLTALLA
                     KVSPVRR"
     gene            3622..4320
                     /locus_tag="VFQ96_07730"
     CDS             3622..4320
                     /locus_tag="VFQ96_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257713.1"
                     /translation="MRFFGRLMRHASHRVDVGTYVPPKNRTLAPVTAVVEEGVLISSY
                     AVRLAIKNQLIVDALRDRADFDVEELKQSAHEHLLALADENDATAARLEARGEDQAPS
                     RPGGTADEVTVTDTGDIVGRSKAIRLLDHRRRPEVHHRLADALRQRALEDDFLAAQVT
                     EAQRAAWREVSGELVRRARGRRSYTPQDSADYARNRAERLRAFVDDDLAALRRAHPDD
                     PGRGGDAEPPDPTK"
BASE COUNT          707 a         1625 c         1516 g          719 t
ORIGIN      
        1 tcgatcctca cgatcgtcgc ccgactgacc gcgcccgtgc cgctcgccgc gcagtcgacc
       61 gccttgaacg cctcccagtc gagcgtcggg tggatcgact tcagcgacca gttcctcggc
      121 atcgtcttcg acctctttcc cgtcgccctc gtgatctttc tgctgtggca gcacgcgccg
      181 tcgcggcacg gcggcgagcg caccctcgcg gccgtctcgg gcacggcgac ctcgatgacg
      241 acgacctcca gcagggccgt gggcacggct ccgaccgacc agggcgcccc gacacccggc
      301 cagtctggat tccggcgaat aggcctcgac ctccgacgcc cgggccacga cctggcactc
      361 ggcgtgctgc tcgtggccgt gatcggcatc ccggggctcg ggctgtacgc ggcgggccgc
      421 gcgctcggga tcaccgtcgc ggtcgacgcc gcaccgacgc actggctgtg gtggaccgtg
      481 ccgatcctcg tcttctcggc gatccgcagc ggcctgcaag aagaggtcat catggtcggc
      541 tacctcttca cccgcctgcc acgggtgggc tgggggccgt ggcgcatcat cctcgcaagc
      601 gccctcgtgc gcggcagcta tcacctctac caggggttcg ggccgttcct cggcaacgcg
      661 gtcatgggcg tcgtcttcgg ctggtattac caccggcacg gtcgcacgat gccgctcgtc
      721 gtcgcccaca cgctgctcga cgtcatttcc ttcgtgggct acccgctcgc gctggcgtgg
      781 tggccggccc tcttcaccac ctgacgccgg cagagccgga ggccacgcac gcgccgtcgt
      841 ggcacgacgc cccacgccgt gacccgcgcg gccgcgagaa tcgcacgaac accggaccgc
      901 acaggagcca ggtcacgcga acgccaggtg gcgtcacccg tgcggcgaca ccgcacagca
      961 gacggaccat cccgaggatc cgactcgccg ggattcgaac cggcccgaaa cgtacgggag
     1021 gggccggccg gccgaagccg gcgaacccct cccgtcgtgg gcagcgtcgc tcagtcgcgc
     1081 ggcgccgggt tgatgatcct gccccatgtc gacttgttgc cccagatacc ctggcgcagg
     1141 ccctccagct ggaggatctg cgaatggtcg acgaagaggt tgatctcgtt gccgtagttc
     1201 ttgatgtacc actcgtagac gggcgggtcg gccgactcga acatgacgtg ctcgatgccg
     1261 acgccgttga tgatctggcc cacgggcgcc gtgttccatt ccacgacgtt ctcggtgatg
     1321 ccctccgact cgatcatgat gatctcggcg ccggcctcca acgccttgtt ggcgcgcgcg
     1381 atcagctcgg cgacgtcctt gtgaccctcg gccgccagct cctcctcgcc ggagtcaccg
     1441 ccggagccga attggatgcc cagctcgggc ttgggcttca gacccgcctt cttgacgagc
     1501 tcgatgaggc gcatgaggcc ggaggtgggc aacgcgatga agccggccga gatctcgatg
     1561 acgtcgaagc cgacctcctt cgcctctttg agatagtggt cgacggcgtc gggcccgtag
     1621 cgcagcaccg tctcgatcca accgccggac gagatgtagg cgtcgtgctc gtgggcgatg
     1681 tcgctgaact tgcgcacctc ctcgcggggc aggagcgcga aggagccgcc cgcccatttg
     1741 acgccgtcga cccactggcc ggcgacctcg agcacgtctg tcagatgcct ggtgccgtag
     1801 gtcgaatagt acggcccgcg gatctcggtg aggccgtaac tgcgcggctt cggtggccgg
     1861 taggcccgtg gaatgaagct gaaactcgtt tcctctgcca tttctcctcc ttcggccggc
     1921 cctttctcgt tcggtccggc ccgttgctcg cccggcacgt caccttgtgg gcgaggccgg
     1981 gcctggagcg gcgcgtcagc gccgcacggg tgacaccttc gccagaagcg ccgtcaggtc
     2041 gcgcacctgc cgcctctcga ggccgcgcac acattcgacg atctcgtcgc gcagcgcggg
     2101 gccggcgaaa tcggtcgcga gggtctcgaa cttggcctgg gcgtcgtccc aatcgaccgg
     2161 atcgttgacg aagccccgat agccgtcgcg gctctcggcc acgacgctgc cgtcctccga
     2221 ctcgacctcg acctcgaccg gcatctcctt cgggaagcgg gcgctgaacc cggcatccgg
     2281 ctgggccttc gtgcgcgcca ggagcgactg cacatcggag gcgaggattc tcttcggctc
     2341 gaactgcgcg ggctgtaccg ccccgtcgag gagtgccgcc gcgaccatgt agagcagcga
     2401 gtggtcagcc tcctccttgg tgcggatctc gcgcttgctg ccttcttcgc cgcccccgat
     2461 gatgtcgtag gccacctgga agatgcggat gcgcaccgcc cggatcttcg ccgggtcgaa
     2521 cccgggcttc tcgcgcagcg ccagggccgc gtcgatcgcg gactgggagt ggatctcggc
     2581 attgtgtttc ttgatgatgg tgcgaagaac gccttcgagg ttctccttcg accagtcgag
     2641 gctgaagtcc ccggcgattg tctgtttgaa gcccttgtta ccctcgaaga cctgcgaagg
     2701 cccggtgatg ccggctcgtg cgaggagcgc cgcgaaggtg ccctccttgg cgacctgcgg
     2761 ataggcgagg cccttccaat tcgacaggtc cccggtgcgg gtcacacgca gcgcgttgtt
     2821 ggcggtgcca gagatcgcga tcgcattggc ggctttcgcg gcgtcgaggc cgagggcacg
     2881 cgagacggcg gccgccaccg cgtaggcgcc ctgcacggtg tggtcgaagc cgtgcgcccg
     2941 caccggggcg acctcgctca gcctcgtttg cacctggtag gcgacggcgg cggctgtcaa
     3001 gaattcgctg cccggggcgt cgacgctctc ggacgcggcc aggcaggcgc cgatgttgtc
     3061 ggagggatgg ttcgtctcac ccggcgagag gaacgcatcc atgaagtcga gataccgact
     3121 gagcgacgtg ttgtagaagg cggcacggtc gggggcgctc gtgccgccgc cgatcagggt
     3181 cgcctggccg ttgccgccga gttcctcggt caggccgcgg atcgcgacag tgggcgtcgc
     3241 gtcgagcgct ccgatggcga caccgagcgt gtcgaggact cggatcttca actggtcgag
     3301 cgccttcgcc gacatatcgg ccagcttcgt tccgacgacg aagcgcgcca ggtcttgtac
     3361 ctctgtcatt tcccctccca cgccgacgca gaacgccggc ctcagacacg gcggaacgag
     3421 accctcgtcg accggatgca cggccagggc gcccttcgac gccgccgcgg ccgggctcgc
     3481 gcccacacag gccgaccgga tcaccctcaa cccttaaaaa tgaaactact ccgtttaata
     3541 gcgggcgtca tgccccgtct tcgccacagg cgcggggcag gcggcccacc accacactcg
     3601 cacgcacggc agaatggtgc cgtgaggttc ttcggccggt tgatgcgtca cgcgtcgcac
     3661 cgcgtcgacg tgggaaccta tgtcccgccg aagaatcgca cgctcgcccc cgtgacggcg
     3721 gtggtcgagg agggcgtctt gatcagcagc tacgcggtgc gcctcgcaat caagaaccag
     3781 ctgatcgtcg acgccctgcg cgaccgcgcc gacttcgacg tggaggagct gaagcagtct
     3841 gcccacgaac accttctcgc gctggcggac gagaacgacg cgaccgccgc ccgtctcgag
     3901 gcccgaggcg aggaccaggc accctcccgg cccggcggca cggccgacga ggtgaccgtg
     3961 accgacacgg gagacatcgt ggggcgctcg aaggcgatcc gcctgctcga tcaccggcgc
     4021 cggccggagg tgcaccaccg cttggccgac gcccttaggc aacgcgcgct ggaagacgat
     4081 tttctcgcgg cgcaggtgac cgaggcccag cgtgcggcct ggcgggaggt gagcggcgaa
     4141 ctcgtgcgcc gcgcccgcgg gcggcgctcc tacaccccgc aagactcggc ggactacgcc
     4201 cggaaccggg ccgagcggct gcgcgccttc gtcgatgacg acctcgccgc cctgcggcgc
     4261 gcccatccgg acgacccggg gcgaggcggc gacgccgagc cgccggatcc cacgaagtag
     4321 cctggcaaca cggagcaact cgacggcacg gaacaatcgg cagcacaaag ccgccggcgg
     4381 cacaaagcca ctcagcagca cagaatcact cggcagcgcg gagcgcaccc acaactccga
     4441 ggggccagga cgttcacgcg aacgcgaaca gcggcggaat cgcggccacc accacgaccg
     4501 accaggccag atacacgggc acataggacg tctgccaccg ctccacggac gcgaacgggc
     4561 gacgccg
//
LOCUS       DASTNL010000239         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_365585, whole genome shotgun sequence.
ACCESSION   DASTNL010000239 DASTNL010000000
VERSION     DASTNL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365585"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VFQ96_07735"
     CDS             <1..1335
                     /locus_tag="VFQ96_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257714.1"
                     /translation="PSLDLFRRRYVEDDVARALGAVAGLGDEAEDLARRLSEAAEGYG
                     RVERAVTGLARSLMAQAAAGTGALLASPLGLVASLAGVADILPGAAALAALFAVLRAA
                     RGVRRGPAGPGVAAREAPDTASRSWPVGKLICSVEFVALLRLAVSSVDDFMLGAAHLP
                     RPIAALLDDDATGLFGLAGSARAFLGLPAVLGLLRETPVRVTKTETMTALPPRGFADL
                     ARRVPAAGPGRPQVRVEEYPTAAGGSRWIVYVGGTVDMAVRGGKEPFDNTSNVHGVAQ
                     SGAAAVRTTVEAMRESGVGVSDPVTLVGYSQGGLAVNGVARVGGYRDVGVVTFGSPTG
                     GVVLPDVKANIAVEIAEDPIPALGGEPAGARQGGLSRTVVTRRIYGSAPPPAGETLPA
                     HDLERYRETAEVMDRSGDPRLVALIDRVAATTGATGSAARGSLWRGERVAAG"
     gene            1346..2452
                     /locus_tag="VFQ96_07740"
     CDS             1346..2452
                     /locus_tag="VFQ96_07740"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0257715.1"
                     /translation="MTGQLPVRLRASIAAATAYRQGRRPPAGGFKLSSNENPYSPLPG
                     VVEAIREAAAYNRYPDATAAALRGPLAARFGVEADGVQVGAGSVAVLAQLIQAASGAG
                     DEVVYPWRSFEAYPGLVTVSGARGVAVPLDSDGRHDLSAMAKAVTGRTRVVIVCSPNN
                     PTGPAVRRAEFDRFLAAIPQDLLVVLDEAYHEFVTDPDAVDGRHLVAGHPNLVIARTF
                     SKAYGLAALRIGYAIGHPAIIRAAQAAAIPLSVTAQAQAGALASLDREDELMERVARI
                     VGRRDRMWHDLVDQGWQLPEAQGNFVWFPTGSSTEAAADVFFDAGLAVRPFSGDGIRV
                     SVGEAESVDVVLRAAKRVLGAQDHGRLADPATQA"
     gene            2743..2979
                     /gene="rpmB"
                     /locus_tag="VFQ96_07745"
     CDS             2743..2979
                     /gene="rpmB"
                     /locus_tag="VFQ96_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU0257716.1"
                     /translation="MAAVCQVTGAKPGFGHSISHSHRRTKRRFDPNVQKKTYYVPSLR
                     RKVTLTVSAKGIKVIDARGIESVVAELLARGEKI"
     gene            2976..3146
                     /gene="rpmG"
                     /locus_tag="VFQ96_07750"
     CDS             2976..3146
                     /gene="rpmG"
                     /locus_tag="VFQ96_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU0257717.1"
                     /translation="MMAKSQDVRPIIKLRSTAGTGYTYVTRKNRRNDPDRLVLKKYDP
                     VVRKHVEFREER"
     gene            3152..3457
                     /gene="rpsN"
                     /locus_tag="VFQ96_07755"
     CDS             3152..3457
                     /gene="rpsN"
                     /locus_tag="VFQ96_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU0257718.1"
                     /translation="MAKKSKIARNKQRQAVVARYAEKRFALKKQLVDPAGSDESHEEA
                     RVALQKLPRNASPVRLRNRDAIDGRPRGVLAQYGVSRVRFREMAHRGELPGITKSSW"
     gene            3699..3986
                     /locus_tag="VFQ96_07760"
     CDS             3699..3986
                     /locus_tag="VFQ96_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0257719.1"
                     /translation="MADKSLNKTELVAAVAATTGQSQSTVAGVVDSLFATIATTVADG
                     GKVTIPGWFAAERTATAARTGRNPQTGAAIQIPAGFRVKLTAGSKLKSAVK"
     gene            4092..5936
                     /locus_tag="VFQ96_07765"
     CDS             4092..5936
                     /locus_tag="VFQ96_07765"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU0257720.1"
                     /translation="MHALAKGESVVFVVAAALGLAAALVFVGGDAVLPPSSQQAAGAS
                     TAAGGLGWGLPALTLVACLAAFGLLGSLLSAFRVAPARAAGRLMSAAGLCAAVLTLAG
                     VAAALTAFVEDSGLSPASAQFSAALGSFVTDTGLGRSWLTTTIAAAVVTTLCFAVRQR
                     PGVAFLVAASLVALAPLAGQQYALGSPGREVGAPAAIAFLVGLAVWSGVTAVAPFARA
                     GGDGAAGAADNAFAARRGRGVFVALACFVVFPSGAIAAAVAVGGAFGSKAGVVTLAAA
                     AFLVVAAGTAAARRPAGRIVASAALGLALACTVVLGYVGQPTGAGTATTGASPAQILT
                     GDAVPDAVGPGFFVRLQPDAIWLSAALVAAVLYAAGVMRLRRARVRWPAWRTVLWMAG
                     LVVTLFLTSGGPALYRRYLFSAQLSVLAAQALLVPLLLVPAAPLTLALTVSRLGGKGP
                     TYRALVAGVRSRAAAFLARPSVAGAILAASLWVWLTTRALDWSASSILGHEVCLAWLF
                     AAGYAFVQSLVGRDPAAYRAAYPARLLVLAAVVASGVAFGLFVGLRHGLWAADWFAAM
                     GRTWGLGAVADQQLGGWLFLLYTSLSGLVLALGVVGVWSRSARDGDRQ"
     gene            complement(5910..>6704)
                     /locus_tag="VFQ96_07770"
     CDS             complement(5910..>6704)
                     /locus_tag="VFQ96_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257721.1"
                     /translation="DMLGTLNDIHVESEQREANGRYLVTASYRIAPVGDGHELRAGHT
                     TFALESAGAIAGVLPRWRFAVSPIAVARVAVRGSTMFTLAGHTLDARAAAGESRPAFS
                     AAGDYLLLTPASVSLRHSNRLTTASPVRLDALTPGALVTATVTARPTPEFTARVRDQL
                     KGYLDSCAEQTVLQPAGCPMGKPIDNRVVGSPTWHITAYPKVSLVAGPSGWIMPPTPG
                     RAEIDVRVQSLFDGSVRDERDTVVFQVSVPKIVIQPDGSLAISVAG"
BASE COUNT          811 a         2353 c         2451 g         1089 t
ORIGIN      
        1 ccctcgctcg acctgttccg ccggcgctac gtggaagacg acgtggcccg ggccttgggc
       61 gcggtcgcgg ggctcggcga cgaggcggaa gacctcgccc gccgtctctc cgaggcggcc
      121 gagggctacg gccgggtcga acgcgccgtc acagggctcg ccaggtcgct tatggcccag
      181 gcggccgccg gaacgggcgc actcctcgcc tcaccgctcg gccttgtcgc ctcgctcgcg
      241 ggtgtcgccg atattctgcc cggcgccgcc gcgctcgccg ctctcttcgc cgtcctgcgt
      301 gccgcccgcg gcgtgcgccg aggcccggcc gggcccggtg tcgcggcccg cgaggcgccg
      361 gataccgctt cgcggtcctg gccggtgggc aagctgatct gctccgtcga gttcgtcgcg
      421 cttctccggc tggccgtctc atcggtggac gacttcatgc tgggtgccgc acacctgcca
      481 cgcccgatcg ccgccctgct cgacgacgac gccaccgggc tcttcggcct ggcaggctcc
      541 gcccgagcct ttctcgggct gccggccgtg ctggggctct tgcgggagac gcccgttcgc
      601 gtgacgaaga ccgagacgat gaccgccctc ccgccccgcg gcttcgccga cctcgcccgg
      661 cgggtgcctg cggccgggcc cggccggccg caggtgcggg ttgaggaata ccccaccgcg
      721 gccggtggga gccgatggat cgtgtacgtc ggcggcacgg tagacatggc cgtccgcggc
      781 ggcaaggaac cgttcgacaa cacctccaac gtgcacggcg ttgcccagtc tggcgcggcc
      841 gccgtgcgca ccacggtgga ggcgatgcgc gagtcggggg tcggcgtcag cgatcccgtg
      901 accctcgtcg ggtactcgca aggcggcctc gccgtcaacg gcgtggcgcg ggtgggcggc
      961 taccgcgacg tcggcgtcgt caccttcggt tcgccgaccg gcggggtcgt cttacctgac
     1021 gtgaaggcga acattgcggt cgagatcgcg gaagacccca ttccggccct cgggggagag
     1081 cctgcgggcg cccggcaggg cgggctctcc cgcaccgtcg tgacgaggag gatctatggc
     1141 agcgcgccac cgcccgccgg cgagacgttg cccgcgcacg atcttgagcg ttaccgcgag
     1201 acggcggagg tcatggaccg ctccggcgac ccgagactcg tcgccctcat cgaccgggtg
     1261 gccgcgacca cgggcgccac ggggagtgcc gcacgcggca gcctgtggcg cggcgagcga
     1321 gtagcggccg ggtagtgtga ggttcgtgac cggtcagtta cccgtcaggc ttcgcgcgtc
     1381 gatcgcggcg gccaccgcgt accggcaggg gcgtaggccg ccggcgggcg gcttcaagct
     1441 ctctagcaac gagaacccgt atagtccgct gcccggggtc gtcgaggcga tccgcgaggc
     1501 cgcggcctac aacaggtacc ccgatgccac cgccgcggcg ctgcgcgggc ccctggccgc
     1561 acgtttcggc gtcgaagcgg atggcgtgca ggtcggcgcc ggttccgtcg cggtgctcgc
     1621 ccagctcatt caagcggcct cgggcgcggg cgacgaggtc gtctacccct ggcgttcttt
     1681 cgaggcgtac cccggcctcg tgaccgtctc cggagcccgc ggcgtggctg tgcccctcga
     1741 ttccgacggt cggcacgacc tctctgccat ggcgaaagcc gtcaccggcc gcacccgggt
     1801 cgtcatcgtc tgctcgccga acaacccgac cgggccggcc gtgcggcgtg ccgagttcga
     1861 ccgcttcttg gccgcgatcc cgcaagacct cctcgtggtt ctcgacgagg cgtaccacga
     1921 gttcgtcacc gaccccgacg cggtcgacgg ccgtcacctc gtggccgggc acccgaacct
     1981 cgtcatcgcc cgcaccttct cgaaggcgta tgggctggcc gccttgcgca tcggctacgc
     2041 gatcggccac ccggcgatca tccgcgccgc gcaggcggcc gcgattccgc tctccgtcac
     2101 ggctcaggcg caggcaggtg ccctcgcctc cctcgatcgg gaagacgagc tgatggaacg
     2161 ggtcgctcgc atcgtcgggc gccgcgaccg gatgtggcac gacctcgtgg accagggctg
     2221 gcagttgccg gaggcgcagg gcaacttcgt ctggttcccg accgggtcct cgacggaggc
     2281 ggccgccgac gtgttcttcg atgccggtct ggcggtgcgg cccttctcgg gcgacggcat
     2341 ccgggtgagc gttggcgagg ccgaatctgt cgacgtggtg ctacgggcgg cgaagcgggt
     2401 tctgggcgcg caagaccacg gccgcctcgc cgaccccgcg acacaggcat gaggctctcg
     2461 tggtggcgcg tcgaacggta ccggcaccgt gccacgccgt gccgaacggc accgcgccga
     2521 acggcggcgc tgcgccggcg gggccgccgt gggcgcccgt gcatcgcacc gcgccacggt
     2581 ttgtgcaggg cgtgggcggc gcgatagggt aagagattgt ccgggtgggc tcctctaccc
     2641 ggttcgtgcg gcctctgctt cgggtttctg gtgtgcgggt cgcgccgaca agagatcttg
     2701 ggcggggcgt cttcggtgcc ccgcggtagc gatggaggaa ccatggccgc agtgtgccag
     2761 gtgacgggag ccaagcccgg cttcggtcac agcatctcgc attcgcaccg gcgtacaaag
     2821 cgccggttcg acccgaatgt gcagaagaag acgtattacg tgccgtcctt gaggcgcaag
     2881 gtgacgttga cggtgtcggc gaagggcatc aaggtcattg acgcgcgcgg catcgagtcc
     2941 gtcgtcgccg agcttcttgc ccgtggggag aagatctgat ggccaagtcg caagacgttc
     3001 gccccatcat caagctgcgc tccacggcgg gcaccgggta cacctacgtg acccgcaaga
     3061 accggcgcaa cgaccccgac cggctcgtgt tgaagaaata cgacccggtg gtgcgcaagc
     3121 atgtcgagtt tcgtgaggag cgctaagact tatggctaag aagagcaaga tcgcccgcaa
     3181 caagcagcgc caggccgtgg tcgcgcggta cgccgagaag cgtttcgcgt tgaagaagca
     3241 gctcgtcgac ccggccggca gcgacgagtc gcacgaggag gctcgggtgg cgttgcagaa
     3301 gcttccccgc aacgcctcgc cggtacggct gcgtaaccga gacgcgatcg acggccgccc
     3361 gcgcggcgtg ctcgcgcagt acggcgtctc ccgagtcagg ttccgagaga tggcacaccg
     3421 cggcgagctg cccggcatca ccaagtcgag ctggtgacgg gggtcgctga ggcgtacttc
     3481 gaaggggcgc aggcttcggt ctgcgcccct ttcgtctttg ttgtgcgagg cctttgcggc
     3541 gaggcgcggc ggctcgtgtg aaatcggccg cgaaggcccg cagatccgtg cgatcacgcg
     3601 gatctctggt acattcgatc cggttaaaga ccgacgtcgt ggctatcgtg gcctcaccgt
     3661 cgaacgatca ctgcacagta cgtccgagga ggacacccat ggctgacaaa tcattgaaca
     3721 agacggagct cgtcgccgct gtcgccgcga cgaccgggca gagccagagc accgtcgcgg
     3781 gcgtcgtcga ctcgcttttc gcgacaatcg cgacgaccgt cgccgacggc ggcaaggtga
     3841 ccattcccgg ctggttcgca gccgagcgca ccgcgaccgc ggctcgcacc ggccgcaacc
     3901 cgcagacggg cgcggccatc cagatcccgg ccgggttccg tgtcaagctg accgccggtt
     3961 cgaagctgaa gtccgcggtc aagtaaggct tgcggccggt gcggccgcgc accgccggcc
     4021 gtccggcagg cgttgccggc ggcccggcgg aggtgccgcg gggctctccg gcagccttag
     4081 gctggacggt gatgcacgcg ttggcgaagg gcgagtcggt cgtcttcgtc gtcgctgcgg
     4141 ccctcggcct ggctgccgcc ctcgtctttg tcggtggcga cgcggtgtta ccgcccagct
     4201 ctcagcaggc ggcaggggcg agcaccgccg ccggcggcct gggttggggc ttgccggcac
     4261 tcaccctcgt tgcctgcctc gcggcattcg gcctgctcgg aagccttctg agcgccttcc
     4321 gcgtcgcgcc cgcccgcgcg gcggggaggc tgatgtcggc ggccgggctg tgcgccgccg
     4381 tcctcacgct cgccggcgtc gcggcggcct tgacggcgtt cgtggaggac agcgggctct
     4441 cgcctgccag cgcccagttc agcgccgcac tcggctcttt cgtcaccgac acggggctcg
     4501 gcagatcgtg gctcacgacg accatcgcgg cggcggtcgt gacgacgctc tgtttcgcgg
     4561 tgcgccagcg cccgggcgtc gccttcctcg tcgcggcttc gctcgtcgcg ctcgctcccc
     4621 tggccggcca gcagtatgcc ctcggcagcc ccggtcgcga ggtgggggcg ccggcggcga
     4681 tcgccttcct cgtcgggctg gccgtatgga gcggcgtcac ggcggtggcg cccttcgccc
     4741 gggcgggcgg tgacggcgcg gccggcgcgg ccgacaacgc tttcgcggcc cggcgcggcc
     4801 gtggcgtgtt cgtggcgctc gcctgcttcg tggttttccc ttcgggtgcg atagccgcgg
     4861 ccgtggcggt cggcggcgcc ttcggctcga aggccggagt cgtgaccctc gctgcggctg
     4921 cgttcctcgt cgtcgccgcc ggcacggctg cggcgcgccg gcccgccggc cgcatcgtcg
     4981 cgtccgcggc tctcggcctc gccctcgcgt gcaccgtggt gctcggatac gtcggtcagc
     5041 cgaccggtgc cggcacggcg acgaccggcg cgagtccggc gcagattctg acgggcgacg
     5101 ccgtgcctga cgcggtgggc ccgggcttct tcgtgcggct gcagcccgat gcgatctggt
     5161 tgagcgccgc gctggtcgcg gccgtgctgt acgcggccgg cgtgatgcgg ctgcgtcggg
     5221 cgcgcgtgcg gtggccggcg tggcgcaccg tgctgtggat ggcgggtctc gtcgtgacgc
     5281 tctttctcac gagcggcggc ccggcgctct accggcgtta cctcttctcg gcgcagcttt
     5341 ccgttctggc cgcgcaggcg ctcctcgtgc cgctcctcct ggttccggcg gccccgctca
     5401 cgctcgcgct caccgtcagc cggctgggcg gcaagggccc gacctaccgg gcgctggtcg
     5461 ccggggtgcg ctcacgcgcg gccgcgttcc tcgcccggcc gtcggtggcc ggcgccatcc
     5521 tcgccgcctc gctgtgggtg tggctgacga cccgagcgct cgactggtcg gcgtcgagca
     5581 tcctcggtca tgaggtgtgt ctggcgtggc tgttcgccgc aggctacgcc ttcgtccagt
     5641 cgcttgtggg ccgggaccct gccgcgtacc gggcggccta cccggcgagg cttctcgtgt
     5701 tggccgccgt cgtggcctcc ggcgtcgcct tcggtctttt tgtcgggctg cggcacggcc
     5761 tgtgggcggc ggattggttc gccgcgatgg ggcgcacgtg ggggctcggc gccgttgccg
     5821 accagcagct cggcgggtgg ctgttccttc tctacacgtc gttgtcgggg ctcgtcttgg
     5881 ccctcggcgt tgtcggagtg tggtcacgct cagcccgcga cggagatcgc cagtgacccg
     5941 tctggctgga tcacgatctt cggcaccgag acctggaaca ccaccgtgtc acgctcgtcg
     6001 cggacagacc cgtcgaaaag ggattggaca cgcacgtcga tctccgcacg gccgggggtg
     6061 ggcggcatga tccacccgga gggcccggcg acgagcgaga ccttcgggta ggcggtgatg
     6121 tgccaggtcg gcgaccccac gacgcggttg tcgatcggtt tgcccatcgg gcaccccgcc
     6181 ggctggagaa cggtctgctc ggcgcagctg tcgaggtagc ctttcagctg atcgcgtact
     6241 cgtgcggtga attccggcgt gggcctcgcg gtgacggtcg ccgtcacgag ggcgcccggc
     6301 gtgagggcgt cgaggcgcac cgggctggcg gtcgtcaggc ggttggagtg gcgcaggctg
     6361 acgctcgcgg gtgtgagcag caggtagtct ccggcggcgg agaacgccgg cctgctctcg
     6421 cctgcggcgg cccgtgcgtc gagagtgtgc cccgccaggg tgaacatggt ggagcctcgc
     6481 accgcgaccc gcgcgaccgc tatagggctg acggcgaagc gccatcgtgg caagacgccg
     6541 gctatcgcac ccgccgactc gagggcgaac gtcgtgtgcc cggcccgaag ctcgtgcccg
     6601 tcgccgaccg gtgcgatgcg gtacgacgcc gtgaccaggt accggccgtt cgcctcccgc
     6661 tgttccgact cgacgtggat gtcgttcagc gtgcccagca tgtc
//
LOCUS       DASTNL010000240         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_182169, whole genome shotgun sequence.
ACCESSION   DASTNL010000240 DASTNL010000000
VERSION     DASTNL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182169"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..604)
                     /gene="ribH"
                     /locus_tag="VFQ96_07775"
     CDS             complement(125..604)
                     /gene="ribH"
                     /locus_tag="VFQ96_07775"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0257722.1"
                     /translation="MSSGGAPEAQTVDGRGLSVVIVAGSWHKEIADGLVAGARRALDA
                     AGAQHRLVQVPGSFELPLAAKAALTAGADAVVALGVIVRGETPHFDYVASAATDGLTR
                     AALDCGKPVGFGVLTCDTEQQGLDRAGLPGSKEDKGAQAAEAALRMVQVLRGLASRS"
     gene            complement(686..2203)
                     /gene="ribA"
                     /locus_tag="VFQ96_07780"
     CDS             complement(686..2203)
                     /gene="ribA"
                     /locus_tag="VFQ96_07780"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEU0257723.1"
                     /translation="MIAKIPQALDALRAGRPVIVADDENRENEGDVILAAQSASQKWL
                     AWTVAHTSGYICAPMTNEIADRLELPVMVPANEDPRGTNYTVSVDAADRTSTGISASD
                     RANTLRTLAAPGSTASSVRRPGHILPLRARDGGVRERGGHTEAAVELLRLAGLTPVAA
                     ICELVADDGEMMRLPDLVKLGAREGIPVVTIPALIDYLNARENRLAHDTTGNAPAKAA
                     AASGATGYAVVTGAPVTPEGHLVEGTGAAGAPQATAEAATGDDALDTPRVTFQVATRI
                     PTRHGPLTVRAYRDRATGADHLAIIAGTPGRQSLVRVHSECLTGEVLGSLKCECGPQL
                     DASLDAIAREGGVVVYLRGHEGRGIGLINKLRAYRLQEDGLDTVDANVALGLPVDARD
                     YGAAAAILADLGIESVRLLTNNPEKQRQLEAHGIRVAERVPLVIPAGPYNAGYLATKR
                     DRMGHLLPADGEPGAAPTPNRAAHPAHTQTASVGDAEEAAPAGDAEAIPAGAASA"
     gene            complement(2200..3087)
                     /locus_tag="VFQ96_07785"
     CDS             complement(2200..3087)
                     /locus_tag="VFQ96_07785"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU0257724.1"
                     /translation="MFTGIIAEVGEITAFWRRDDSARVTVRAPGATAGAKTGHSIAVS
                     GVCLTVVSRKGDTFQADVMAETLAVSTFGAALGAALGTATGSADHPAAHSITAVQAEA
                     ADQGEARAAADTTSGEEAHPAAVLTARPPSGRGLPVNIESALRVGDPLGGHIVQGHVD
                     GTATLLTRADGVRWRVLRFSLAPRLAPLVAYKGSVALDGVSLTVSGVGDDWFEVSLIP
                     ETIAATTLGALAEGDSVNLETDILARHIRRLSAFGRLAGDDEPWGAPAAGTEASRGGG
                     GAHVREHAGDEPAGNEENR"
     gene            complement(3090..4223)
                     /gene="ribD"
                     /locus_tag="VFQ96_07790"
     CDS             complement(3090..4223)
                     /gene="ribD"
                     /locus_tag="VFQ96_07790"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU0257725.1"
                     /translation="MRDDEAMRRALALAANGPSTGVNPRVGCVILSPDGEVVAEGWHR
                     GAGTAHAEVDALSHCAAPAGCTAVVSLEPCNHTGRTGPCSEALIAAGIRRVVYAVPDP
                     TARAAGGARRLAEAGVEVTGGVCLAEAEAFLGDWLVAARLGRPFVTVKWAASLDGRVA
                     ADDGSSQWITGGPARRRVHEQRAANDAVIVGTGTILADDASLTARRDDGSLLPDQPIP
                     VVVGMRQIPEGAAVRRHPHPPIFAATHDLRALIGRLMGLGVRTVYVEGGPTLASAFIR
                     QGLADRYRVFVAPTLLGGARTAIGEIGVASLAAARPLEFTSVERLGADLLITAVPAGE
                     TGRARTGEGKHSDPSVSEARQARTIAGGDEATGETAARPGNGG"
BASE COUNT          518 a         1608 c         1537 g          637 t
ORIGIN      
        1 gctgacggcc gaactggcca gtatgcgcta caagctcacc tgttgagccg gacgcccggg
       61 cgcgccgcaa tcgcgcgcac ccggggcgaa gcgtgcggcc tcgactcgcg gcggactcgg
      121 actctcacga ccgcgaggcg aggccgcgca acacctgcac cattcgtagt gcggcttcgg
      181 ccgcctgagc gcccttgtcc tctttcgagc cggggagccc ggcgcggtcg agtccctgct
      241 gctccgtgtc gcaggtgagc acgccgaagc cgaccggctt gccgcaatcg agagcggccc
      301 gggtgaggcc gtcggtcgcc gcggaggcga cgtagtcgaa atgcggggtc tctccgcgca
      361 cgatcacgcc caaggcgacg acggcatcgg cgccggccgt gagcgccgcc ttcgcggcga
      421 gcggcagttc gaagctgccc ggcacttgca caagccggtg ctgggcgccc gccgcgtcga
      481 gcgcccgccg ggcgcccgcc acgagaccgt cggcgatttc cttgtgccag ctcccggcga
      541 cgatgacaac gctcagcccc cggccgtcga ccgtttgcgc ctcgggcgct ccccctgaac
      601 tcatgctctc tcctcgttgg cgcgcccgct ggttgcgggc ctggtctgct gcgcaggtgg
      661 cggggcgaac cttcccggca tgtgatcacg ccgacgcggc ccccgcagga atggcctcgg
      721 cgtcgcccgc cggggccgct tcctccgcgt cgcccaccga ggctgtttgc gtgtgggcgg
      781 gatgagcggc gcggttcggc gtcggggcgg cgcccggttc gccgtcggcc ggcagcaggt
      841 gccccatccg gtcgcgtttc gtcgccaggt agccggcgtt gtaggggccg gccgggatca
      901 cgagcggcac gcgctcggcc acccggattc cgtgtgcctc gagctgccgt tgcttctcgg
      961 ggttgttcgt caagagccgc accgactcga tgccgaggtc ggcgaggatc gccgccgccg
     1021 cgccgtaatc ccgtgcgtct accggcaggc cgagggcgac gttcgcgtcg accgtgtcga
     1081 ggccgtcttc ttgcagccgg taggcgcgca gcttgttgat gaggccgatg ccgcggccct
     1141 cgtggccgcg caggtagacg acgaccccac cctctcgggc tatcgcgtcg agcgaggcgt
     1201 cgagctgcgg gccgcattcg catttcagcg agccgagcac ctcgccggtc aggcattcgg
     1261 aatgcacccg cacgaggctc tgccggcccg gggtgccggc gatgatcgcc aggtggtccg
     1321 cgccggttgc ccggtctcgg taggcgcgca ccgtcagcgg gccgtgccgg gtgggaatgc
     1381 gggtcgcgac ctggaacgta acgcgtggcg tgtcgagcgc gtcgtcgccg gttgccgcct
     1441 cggccgttgc ctgtggagca cccgccgcgc cggtgccctc cacgaggtgc ccttctgggg
     1501 tgacaggcgc acctgtcacg acggcgtagc ccgtggcgcc gctcgctgcc gccgccttag
     1561 ccggagcgtt tcccgtggtg tcgtgtgcca ggcggttctc ccgggcgttc aggtagtcga
     1621 tgagggcggg gatcgtgacg accgggatcc cctctctcgc gccgagcttc acgaggtcgg
     1681 gcagcctcat catttcgccg tcgtcggcca ccagctcgca gatcgccgcc accggggtga
     1741 ggccggccag cctcagaagc tcgaccgccg cctccgtgtg gccgccgcgt tcccgcacgc
     1801 cgccgtcgcg ggcgcgcagc ggcagaatgt ggccgggccg gcgcaccgaa ctcgcggtgg
     1861 agccgggcgc cgccagcgtg cgcagtgtgt tcgcccggtc gctcgcgctg atgcccgtgc
     1921 tcgtgcggtc ggccgcgtcg acgctgaccg tgtagttcgt gccgcgcgga tcctcgttcg
     1981 ccggcaccat gacgggtagt tccagccggt cggcgatctc gttcgtcatc ggcgcgcaga
     2041 tgtagccgga ggtgtgggcg accgtccacg ccagccactt ctggctggcg ctctgcgcgg
     2101 cgaggatcac gtcgccctcg ttctcgcggt tctcgtcgtc ggccacgatc accgggcgcc
     2161 cggcgcgcaa tgcgtccagc gcctgcggaa tcttcgcgat catcggttct cctcgttccc
     2221 tgccggctca tcgccggcgt gttcacgcac gtgtgccccg ccgcccccgc ggctcgcctc
     2281 cgtgccggcg gcaggggcgc cccagggctc gtcgtcgccc gcgagccggc cgaaggcgct
     2341 caaccgacgg atgtgtcgcg ccagaatgtc ggtctccagg ttcacagagt cgccctcggc
     2401 aagcgcgccg agcgtcgtcg cggcgatcgt ctcgggaatg agcgacacct cgaaccagtc
     2461 gtcgccgact ccgctgaccg tcagcgaaac cccgtcgagc gcgacggagc ccttgtaggc
     2521 gacgagcggc gcgagccgcg gcgcaaggct gaagcgcagc acccgccagc gcacgccgtc
     2581 ggcgcgggtg agaagcgtcg ccgtgccgtc cacgtgaccc tgcacgatgt ggccgcccag
     2641 cgggtcaccg acccgcaacg ccgactcgat gttgacgggc aggccccggc cacttggcgg
     2701 gcgcgccgtc aggaccgccg ccgggtgcgc ttcttcgccg cttgtcgtgt cggccgccgc
     2761 gcgtgcttcc ccttggtcgg cggcctccgc ctgcacggcc gttatcgagt gcgccgccgg
     2821 atggtcggcc gaaccggttg ccgtgccgag cgccgcaccg agcgctgcac cgaaggtgct
     2881 gacggcgagg gtctccgcca tgacgtcggc ctgaaacgtg tcgccttttc gactgacgac
     2941 ggtgaggcac acgccggaga cggcgatcga gtggcccgtc ttcgcgcccg ccgtggcgcc
     3001 cggcgcccgc accgtgacgc gcgccgagtc gtcccgccgc cagaacgccg tgatctcgcc
     3061 gacctccgcg atgatgcccg tgaacatccc tacccgccgt tccccggccg cgcggccgtc
     3121 tcgcccgtcg cctcgtcgcc gccggcaatc gttcgtgcct gccgagcctc gctcacggag
     3181 gggtccgagt gcttgccttc gccagttcgc gcccgacccg tttcaccggc cggcacagcg
     3241 gtgatgagga gatccgcgcc gagccgctcc acgctcgtga attccagcgg ccgcgcggcc
     3301 gcgagcgagg cgacgccgat ctccccgatc gccgtgcgcg ccccgccgag cagggtcggc
     3361 gccacgaaca ctcggtagcg gtcggcgagg ccctgccgga tgaacgccga ggcgagggtc
     3421 ggcccgccct ccacgtagac ggtgcgcacg ccgagcccca tcagccggcc gatgagcgcc
     3481 ctcaggtcgt gggtcgcggc gaagatcggc ggatgcgggt gccggcgcac cgccgccccc
     3541 tccgggatct gccgcatgcc cacgacgacg gggatcggct ggtcgggcag cagggatccg
     3601 tcgtcacgcc gtgccgtcag gctcgcgtcg tcggcgagaa tcgtgccggt gcccacgatc
     3661 accgcgtcgt tggccgcgcg ttgctcgtgc acccgccggc gggccgggcc gccggtgatc
     3721 cactggctgg agccgtcgtc ggccgccacc cgcccgtcga ggctggcggc ccatttcacc
     3781 gtgacgaacg gtcggccgag ccgcgcggcg acgagccagt cgccgagaaa cgcctccgcc
     3841 tctgccaggc acactccgcc cgtcacctcg acgcccgcct cggcgaggcg ccgggccccg
     3901 cccgcggccc gcgccgttgg atcgggtacc gcgtagacga cgcggcggat gccggcggcg
     3961 atgagcgcct cggaacaagg gcccgtacgg cccgtgtgat tgcaaggctc cagcgagacg
     4021 acggccgtgc atcctgccgg ggcggcgcag tgcgagagcg cgtcgacctc ggcatgcgcg
     4081 gtgccggccc cgcgatgcca cccttccgcg accacctcgc cgtcggggga gaggatgacg
     4141 cagccgaccc gcgggttcac gccggtcgag ggtccgttcg ccgcaagggc cagggcgcgt
     4201 cgcatcgcct cgtcgtcgcg catggccctc ccccgtgcta cgtgagaatc acgtggcgtc
     4261 cgcgtgcggg cggggcgaaa ggcatgacaa aaccgtgctg
//
LOCUS       DASTNL010000241         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_182191, whole genome shotgun sequence.
ACCESSION   DASTNL010000241 DASTNL010000000
VERSION     DASTNL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182191"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..698)
                     /gene="rplD"
                     /locus_tag="VFQ96_07795"
     CDS             complement(36..698)
                     /gene="rplD"
                     /locus_tag="VFQ96_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU0257726.1"
                     /translation="MAATIDVLDAQGGTTGSVDLPAALFDAQTNIPLIHQVVVAQLAA
                     ARQGTHKTKTRGEVSGAGRKPFKQKGTGNARQGSIREPQMTGGGIVHGPVPRSYAQRT
                     PKKMIAAALFGALSDRARGGRIHVVETLGAGETPSTKAAAAQLAGVTAAGSKRTLVVL
                     AAEEETGYRSVRNLPQVHTLTFGQLNAYDVLVADDIVFTKAAFDAFVAARAATTAEEA
                     GK"
     gene            complement(703..1359)
                     /gene="rplC"
                     /locus_tag="VFQ96_07800"
     CDS             complement(703..1359)
                     /gene="rplC"
                     /locus_tag="VFQ96_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU0257727.1"
                     /translation="MSENLTRTTKGLLGTKLGMTQVWDENNRVVPVTVIEVGTNVVTQ
                     LRSVENDGYSAVQIAYGRVDPRRVDKPTTGHFAKAGVTPRRHLAEVRTDDAANYSVGQ
                     ELTADTFTAGELVDVVGTSKGKGFAGNIKRHNFSGVSASHGAHRNHRKPGSIGASSTP
                     SRVFKGWRMAGRMGGERVTVLNLTVQAVDLEKGLLLVKGAVPGARGRLVFVRNAIKGV
                     "
     gene            complement(1383..1691)
                     /gene="rpsJ"
                     /locus_tag="VFQ96_07805"
     CDS             complement(1383..1691)
                     /gene="rpsJ"
                     /locus_tag="VFQ96_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0257728.1"
                     /translation="MAGQKIRIRLKSYDHEVIDTSAGKIVDTVTRAGATVIGPVPLPT
                     EKNVVCVIRSPHKYKDSREHFEKRTHKRLIDIIDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            complement(2203..>2729)
                     /locus_tag="VFQ96_07810"
     CDS             complement(2203..>2729)
                     /locus_tag="VFQ96_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU0257729.1"
                     /translation="ITGRGTVVTGRAERGTLAVNSEVEIVGIRPTQKTTVTGIEMFHK
                     QLDEAWAGENCGLLLRGTKREEVERGQVVAKPGTVTPHTEFEGTAYVLSKDEGGRHNP
                     FYTNYRPQFYFRTTDVTGVITLPEGTEMVMPGDTTDMKVDLIQPIAMEEGLGFAIREG
                     GRTVGAGTVTKVVK"
BASE COUNT          336 a          913 c          989 g          491 t
ORIGIN      
        1 gaggatgatc tcgcgcggat ccttgttgac ggcggtcatt tgccggcctc ctcggctgtg
       61 gtcgcggcgc gggcggcgac gaacgcgtcg aaggccgcct tggtgaagac gatgtcgtcg
      121 gcgacgagga cgtcataggc gttcagctgg ccgaaggtga gcgtgtgcac ctgaggaagg
      181 ttccgcacgc tgcggtagcc cgtctcctcc tcggccgcga ggacgacgag ggtcctcttg
      241 gagcctgccg cggtgacgcc cgccagctgg gcggcggccg ccttcgtcga cggggtctcc
      301 ccggcgccga gggtctcgac gacgtggatg cgcccgccgc gggcccggtc ggagagcgcg
      361 ccgaacaggg cggccgcgat catcttcttc ggggtgcgct gcgcgtagct gcgcggcacc
      421 ggcccgtgga cgatgccgcc gccggtcatc tgcggttcgc ggatcgagcc ctgacgcgcg
      481 ttgccggtgc ccttctgctt gaagggcttg cggccggcgc cggagacctc gcctcgggtc
      541 ttggtcttgt gagtgccctg gcgcgccgcg gccagctggg cgacgacgac ctggtggatc
      601 agcgggatgt tcgtctgcgc gtcgaagagc gcggcgggca ggtcgaccga cccggtcgtg
      661 ccgccctgtg cgtcgaggac gtcgattgtg gcagccatcc ggtcacactc ccttgattgc
      721 gttgcggacg aagacgaggc ggccgcgggc tccggggacg gctcccttga cgagcaggag
      781 gcccttctcc aggtcgacgg cctgcacggt gaggttcagg accgtcacgc gctcaccgcc
      841 catgcggccg gccatccgcc agcctttgaa gacccggctg ggcgtcgagg aggctccgat
      901 cgagccgggc ttgcggtggt tgcggtgcgc gccgtgggag gcggagacgc cggagaagtt
      961 gtggcgcttg atgtttcccg cgaagccctt gcccttggag gtgccgacga cgtcgacgag
     1021 ctctcccgcg gtgaaggtgt cggccgtgag ctcctggccg acgctgtagt tcgcggcgtc
     1081 gtccgtgcgc acctcggcga gatggcggcg cggggtgacc ccggccttcg cgaaatgccc
     1141 tgtggtcggc ttgtcgacgc gacgcgggtc gacgcggccg taggcgatct gcacggcgct
     1201 gtagccgtcg ttctcgacgg agcgcagctg ggtgacgacg ttcgtgccca cctcgatgac
     1261 ggtgaccggc acgacccggt tgttctcgtc ccacacctgg gtcatgccga gcttggtgcc
     1321 cagcaggccc ttggtggtcc tggtcaggtt ctcagacatc tctcttcgtg cccccggccg
     1381 gatcagagct tgatctcgat gttgacgtcg gcgggcaggt cgagccgcat gagcgagtcg
     1441 acggccttcg gcgtcgggtc gatgatgtcg atgagccgct tgtgcgtgcg cttctcgaag
     1501 tgctcgcggc tgtccttgta tttgtgcggc gagcggatga cgcagaccac gttcttctcg
     1561 gtgggcagcg ggaccgggcc gatgacggtc gcgccggccc gcgtgacggt gtcgacaatc
     1621 ttgcccgccg aggtgtcgat cacctcatgg tcgtacgact tcaggcgaat gcggatcttc
     1681 tgtcccgcca tgtcggactc gctctctttc tcaggcgtca tacggccggg accgcattgg
     1741 acgcgcgtgg cgttcggcgg ccttcgccgc tctcccaccg ctgttcatct gtcaaggccg
     1801 ggatcggatg cccggccccg gcgccccgcg acacacgtca tcggcgctgc cgagacctgg
     1861 cgtctcggcc gccgatcaag ctgtgccctt ggaggagagg ggtgcctctc cggggctctg
     1921 ctctgccgcc tgcggcctga cctcaccctt gcgggccggc catgcactgc caggcagtga
     1981 tccgccgtga cgcaggcgtc acggcgaaat gtcgaactag acgagtgtgc cacatcgccc
     2041 aggaccgtgc aacccgggcg tgtcgcactc cccatgcccc ggtccgcctg cccgggcggc
     2101 gcgggagccc tcccggcgga ggctgccccg gagacggcga aggggccgag ccctgcggct
     2161 cggccccgac gtcgtgtccc agacgggccc agacggatgc tgctacttga cgaccttcgt
     2221 caccgtgccg gcgccgacgg tgcggccgcc ctcgcggatg gcgaagccga ggccctcttc
     2281 catcgcgatc ggctggatca ggtcgacctt catgtcggtc gtgtcgcccg gcatgaccat
     2341 ctcggtgcct tccggcaggg tgatgacgcc cgtgacgtcc gtggtgcgga agtagaactg
     2401 cggccggtag ttcgtgtaga aggggttgtg gcgcccgccc tcgtccttcg agaggacgta
     2461 agccgtgccc tcgaactcgg tgtgcggggt gacggtgccg ggcttggcga cgacctggcc
     2521 gcgctcgacc tcttcgcgct tggtgccgcg caggagcagg ccgcagttct cgccggccca
     2581 cgcctcgtcg agctgcttgt ggaacatctc gatcccggtg accgtcgtct tctgcgtcgg
     2641 gcggatgccg acgatctcca cctccgagtt gacggcgagg gtcccgcgct cggcgcggcc
     2701 cgtgacgacc gtgccgcggc cggtgatcg
//
LOCUS       DASTNL010000242        11009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_738536, whole genome shotgun sequence.
ACCESSION   DASTNL010000242 DASTNL010000000
VERSION     DASTNL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738536"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(496..1095)
                     /gene="nadD"
                     /locus_tag="VFQ96_07815"
     CDS             complement(496..1095)
                     /gene="nadD"
                     /locus_tag="VFQ96_07815"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU0257730.1"
                     /translation="MPQEERRPRIGVMGGTFDPIHHGHLVAASEVARSFDLDQVIFVP
                     TGDSWQKRHVTPSEHRYLMTVVATASNPRFTTSRVDIDRGGPTYTIDTLRDIHARHPD
                     AELYFITGADAVAQIVGWHDSDELWGLAHFVAVTRPGHELDVSVLPDHRISRLEVPAL
                     AISSTDCRSRVRRGYPVWYLVPDGVVQYIAKHQLYRSAA"
     gene            complement(1106..1306)
                     /locus_tag="VFQ96_07820"
     CDS             complement(1106..1306)
                     /locus_tag="VFQ96_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257731.1"
                     /translation="MLATVVSAAAEQTFPMVAPIWVFGVLPLVVFAVLGLIMWSYHDV
                     ANRHSRKARAYAAGRSANGQQH"
     gene            complement(1384..2697)
                     /locus_tag="VFQ96_07825"
     CDS             complement(1384..2697)
                     /locus_tag="VFQ96_07825"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU0257732.1"
                     /translation="MSTPVISDAATAALPGPAAGTLPAEIVDRLTRAKNASRALAAAT
                     TSTKNDALAAIARRIGEESGRIVAANHDDLARGRADGLSAGLLDRLTLGPARLEGLAT
                     AVRKLIDLADPVGETVRGHVLPNGLVVSQVRVPFGVIGAIYEARPNVTIDLATLAIKS
                     GNAIVLRGGGAAKATNAVLLDVLRGGLTAAGLPGDAVQSVDDFGRPGGTALMHARGYI
                     DVLVPRGSAGLIQTVVTESSVPVIETGAGVVHIVVDASASEKEAVEIIHNAKVQRPSV
                     CNAVETVLIHEDAAARLAAPVLDRLAASGVTVHADERIRSLWPKALPITEEDWGVEHM
                     GLEVNVGVVHDLDEAIAHIARYSTGHTESIVTNDLANAERFLNEVDSAVVMVNASTRF
                     TDGGEFGFGAEVGISTQKLHARGPMGLTELTSTKWLVRGNGQIRA"
     gene            complement(2707..3552)
                     /gene="proB"
                     /locus_tag="VFQ96_07830"
     CDS             complement(2707..3552)
                     /gene="proB"
                     /locus_tag="VFQ96_07830"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU0257733.1"
                     /translation="MILEKRHDAARARRVVVKVGSSSVSGANAGQIGMLVDALAERYG
                     AGGEVVLVSSGAIATGTPYLHLDDRPIDLATQQAAAAVGQNVLIYRYQDSLDRYGILA
                     GQVLLTAGDLQDPTARSNARRAMERLLTLRILPIVNENDTVATHEIRFGDNDRLASFV
                     ATLLHADLLVLLSDVDALYTRPPAKPGARRIDVVRCAEDLDGVEIGGVGSGVGTGGAA
                     TKVAAALHAAEAGTAVLFTSAVQVREALAGREVGTWFVPAAPAPTGDTGSRLGREQGL
                     AAGAS"
     gene            complement(3549..5087)
                     /gene="obgE"
                     /locus_tag="VFQ96_07835"
     CDS             complement(3549..5087)
                     /gene="obgE"
                     /locus_tag="VFQ96_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU0257734.1"
                     /translation="MAHFVDTVTLHVSAGDGGDGCVSIRREKYKPLAGPDGGNGGHGG
                     DIVLAAESQVTTLLDYRRRPHRASDSGGFGMGGGRHGQRGEDLVLPVPVGTVVRTADG
                     TLLADLTEPGMRVVVAEGGIGGLGNAALATTKRKAPGFALLGTPGWSGDILLELKTLA
                     DAALVGFPSAGKSSLIAALSAARPKVADYPFTTLRPHLGVVQAGQSRYTIADVPGLIE
                     GASAGKGLGLEFLRHVERCSVLVHVVDCATPEEGRDPLSDLDVILRELATYPVAEGRT
                     PLGQRPQIVVLNKIDVPEARELAQFVRGMIEERGLPVFEVSAVSREGIRALSFAIAER
                     VEAERGRHERPEPARILLRPRPVNDPGFTVVREGGAEPVFRVRGVKPERWVAQTDFAN
                     DEAVGYLADRLLRLGVEEALLAAGAVAGSTVVIGQGAGVVFDWEPALTSAAELVTGPR
                     GTDERVEGRARATHRERRADYLTWMDAKAAARAELEEERKAGIFPPAEATDEPSPDTG
                     PGAS"
     gene            complement(5170..5421)
                     /gene="rpmA"
                     /locus_tag="VFQ96_07840"
     CDS             complement(5170..5421)
                     /gene="rpmA"
                     /locus_tag="VFQ96_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU0257735.1"
                     /translation="MAHKKGASSSRNGRDSNAQRLGVKRFAGQSVNAGEILVRQRGTH
                     FHPGVNVGRGGDDTLFALSAGEVEFGQKGRRKVVNIVSA"
     gene            complement(5443..5751)
                     /gene="rplU"
                     /locus_tag="VFQ96_07845"
     CDS             complement(5443..5751)
                     /gene="rplU"
                     /locus_tag="VFQ96_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU0257736.1"
                     /translation="MVYAVVRAGGRQEKVEVGTVVTMNRVEADESGTVRLPAVLLVDG
                     DTVTSDRTVLKNVTVTAEVLENLRGKKIVIQKFKNKTGYRKRQGHRQELTRVKVTGIK
                     "
     gene            6012..6341
                     /locus_tag="VFQ96_07850"
     CDS             6012..6341
                     /locus_tag="VFQ96_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HEU0257737.1"
                     /translation="MTVLIDRPLWPAHGTVFSHLVSDASLHELHTFATRHGIPRRAFD
                     LDHYDVPVSTYAELVSAGALEVTGRQLVLRLAASGLRVRGRDRPSRARAGASLVERPV
                     ARRLHMQ"
     gene            complement(6297..8783)
                     /locus_tag="VFQ96_07855"
     CDS             complement(6297..8783)
                     /locus_tag="VFQ96_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU0257738.1"
                     /translation="MVEKNKDRNTTDETNNGAGEPRGFGRLFGGHRSARRTARRVSVA
                     SPGRDSEPATGRPGTRQAEAAPDDGETRQAAVEGEDSVARSGAGVGRARGDAAAEGGR
                     AAADVPGGPQTNDPTADGMQAGAASASDADGDVVETNDTGTNDTGGADAAQGDARGSD
                     ALLPQVTRPDGLPPATILFMAPEVLPGWHEADGDLEEPETSHRRRSRHRSGRDDRDGD
                     RPQPGRRDTDRQTRPLITEPQKIKGSTRLEAKKQRRRDGRADSHRRQVITEVEFLARR
                     ESVDRAMVVRERQDRIQIGVLEDGVLVEHYVARSQEASLIGNVYLGRVQNVLPSMEAA
                     FVDIGRGRNAVLYSGEVDWQAAAERLGSDKAHRIENALKPGDTVLVQVTKDPVGHKGA
                     RLTSQVSLPGRYLVYVPGGSMNGISRKLPDTERARLKKILKALLPEDAGVIVRTAAEG
                     ATDDQLTRDVDRLRGQWDAISAQVQGGQAPLLLHSEPDLLIKIVRDIFNEDFHRLVVD
                     GDEARATIEHYLSQVAPDLVEKVERYDGDDDSFDHYRIAEQIEKALERKVWLPSGGTL
                     IIDRTEAMTVIDVNTGKFVGSGGNLEETVTKNNLEAAEEIVRQLRLRDIGGIIVVDFI
                     DMVLESNRDLVLRRLVECLSRDRTKHQVAEVTSLGLIQMTRKKIGLGLLETFGEPCEV
                     CAGRGVVVHHDPVLKHKVPAQTKRKASSRQAAAKTTHALTEKAKKGLAQIAASTLSHP
                     SGTAEDQIAENETGGARHESEQAVAILDIPIAPSRRRQRVSTSEAEHLLDSVLDALPQ
                     PKKATPPRTRRRVTTGSLHVEAAGDGSLDE"
     gene            9072..9671
                     /locus_tag="VFQ96_07860"
     CDS             9072..9671
                     /locus_tag="VFQ96_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU0257739.1"
                     /translation="MPEPGTVKKPTALAIFLIVAGTIGLAAAFLLTNDKFTLLQNPHA
                     QLNCNFSVLVGCSKNLTEWQGSVFGFPNPVLGLMCWPVPITVGVATLAGARLPRWFWL
                     LFNLFVLAAVVLVGWFIWQSVFALFVLCPWCMVTWAVTIPTFWALTLHNLSSGHIPLA
                     PRARRVFHTLYSWTPVIVLLSYAFVAVLAQIQLDWLHRL"
     gene            complement(9730..10149)
                     /gene="ndk"
                     /locus_tag="VFQ96_07865"
     CDS             complement(9730..10149)
                     /gene="ndk"
                     /locus_tag="VFQ96_07865"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU0257740.1"
                     /translation="MGNDEEETLVLVKPDGVARGLTGEILRRIEAKGYRITGLTMRLA
                     DRDLLARHYAEHVGKPFYEPLVEFMLSGPIVAARVRGHRAIAGFRSLAGATDPTAAAP
                     GTIRGDLGRDWGLAVQQNLVHGSDSPASAARELALWF"
     gene            complement(10210..10503)
                     /locus_tag="VFQ96_07870"
     CDS             complement(10210..10503)
                     /locus_tag="VFQ96_07870"
                     /inference="COORDINATES: protein motif:HMM:NF025383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEU0257741.1"
                     /translation="MLGFEVVVVFLGMLVVFGLKALPPAQAFLVGGAVILVMLVALVL
                     LPHRIAYVLGWLTQLAVVAGGFLIGMLFVVGIIFTAIWTWCMVTGIRLDRAEA"
     gene            complement(10560..>11009)
                     /locus_tag="VFQ96_07875"
     CDS             complement(10560..>11009)
                     /locus_tag="VFQ96_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU0257742.1"
                     /translation="PGRLQVIGADPTVLVDAAHNPGGAASLAAALDDYFHLGEIAVVF
                     GVMADKDLPGILDHVGPHGSVFFASQSHSSRALGHSDLARKLASRLGDQRVHDAGDVA
                     DALEAARAWAGAASGRAVLVTGSIALVGDALGVAAEHGWLRETGVRA"
BASE COUNT         1594 a         3931 c         3685 g         1799 t
ORIGIN      
        1 ccgcctcgcc gccaggaagg cgcgcgcctc cacccggcgt ctggcgtgca gcctcgccgg
       61 aatctgccag cacggccgca cgacgcgcac gacgtgtgag gagaagcgca gacggcgaag
      121 cgtcgcccgt gggacgggcc ggctggatgc cgccggccgc cggagaggag ccccgcgtcg
      181 tcgctgaagc cgcgccccgc gcagtagtgg gatccggcgt cgcctgccgg gcggagcccc
      241 gagggacaga ccaccccgaa gtgggcgccc gccgactcgc cggggcaggc ggaaccgcgt
      301 ctgacgtacc cacggcgtgt gcggcccgac ggccatggcc cggcccggca gcacgggcct
      361 cttgcacggc acgggccgca tcgtcgggcc cgacttggcc cgggtctttc ccggcccgtt
      421 cggcagcctg acgctcctct tggcgctccc ggtcccgcag ttcccgccgc gtcaacggtg
      481 ggcggtccca ggcactcatg cggcactccg atacaactga tgcttggcga tgtactgaac
      541 gaccccgtcc ggcacgagat accagacagg gtacccccgc cgcacgcggc ttcggcaatc
      601 cgtcgacgag atcgccaacg ccggaacctc gaggcgactt atgcggtggt cgggcaacac
      661 cgagacatca agctcgtggc cgggccgggt gacggcgacg aaatgcgcaa gcccccacaa
      721 ctcgtcagaa tcgtgccatc ccacgatctg cgcgaccgcg tcggccccgg tgatgaagta
      781 caactcggcg tcgggatgcc gtgcgtgaat gtcgcggagg gtgtcgatcg tgtaggtggg
      841 cccgccacga tcgatgtcga ctcggctcgt cgtgaaccgc gggttcgacg ccgtcgcgac
      901 aaccgtcatc aggtagcggt gctcgctggg cgtgacgtga cgcttctgcc acgagtcgcc
      961 cgtcggcacg aagatcacct ggtcgaggtc gaacgagcgc gcgacctcgc tggcggccac
     1021 gaggtgcccg tgatgtatgg ggtcgaaggt tccgcccatc accccgatac ggggacgccg
     1081 ctcttcctgt ggcatcgctt acaggtcagt gctgctgccc gttggcgctc cgcccggccg
     1141 cgtaggcgcg cgccttacgg ctgtgccggt tggccacgtc gtggtaactc cacatgatga
     1201 ggccgagaac ggcgaagacg acgagcggaa gaactccgaa aacccagatc ggcgccacca
     1261 tcgggaaggt ctgttcggct gccgccgaga ccacggtcgc tagcacgtgt gcatcctttc
     1321 ttcggcacgg cctgtgccac ccacaacact accgggcgaa cacctgcccg acgcgcctgt
     1381 cgttcaggcg cggatctgcc cgttgccgcg caccagccac ttggtgctcg tcagctccgt
     1441 cagccccatc ggcccgcgag catgcaactt ctgagtggag atgcccactt cggcgccgaa
     1501 gccgaactct cccccgtcgg tgaatcgggt agaggcgttc accatgacca ccgccgaatc
     1561 cacctcgttt aaaaaccgtt ccgcgttggc gaggtcattt gtcacgatcg actcggtgtg
     1621 gcccgtcgaa tagcgggcga tatgagcgat cgcctcgtcg agatcgtgca ccacgccgac
     1681 gttgacctcg agccccatgt gctccacccc ccagtcttcc tcggtgatgg ggagggcctt
     1741 cggccacagg ctgcggatgc gttcgtcggc gtgcacggtg acgcccgaag cggcgagccg
     1801 gtcgagcacg ggcgcggcaa gccgtgccgc ggcgtcttcg tggatcagca cggtctcgac
     1861 ggcgttgcac acactcggcc gctgcacctt ggcgttgtgg atgatctcga ccgcctcttt
     1921 ctcggaggcg gaggcgtcga cgacgatgtg tacgacgccg gcgccggtct cgatgacggg
     1981 cacgctcgac tcggtgacga ccgtctggat gagtcccgcg gagccgcgcg gcacgagcac
     2041 gtcgatgtac ccgcgggcat gcatcagggc tgtgccgccc ggcctgccga agtcgtcgac
     2101 gctctgcacc gcgtcgccgg ggaggcccgc cgcggtgagg ccgccccgca gcacgtcgag
     2161 gagcaccgcg ttcgtcgcct tcgccgcgcc gccgccgcgt aagacgatgg cgttgccgct
     2221 tttgatggca agcgtcgcga ggtcgatcgt cacgttcggg cgcgcctcgt agatagcgcc
     2281 gatgacgccg aacggcacgc gaacctggct cacgacgagc ccgttgggca acacgtggcc
     2341 gcgcaccgtc tcgccgaccg ggtcggccag gtcgatgagc ttgcgcacgg ccgtggcaag
     2401 accctcgagg cgggccgggc cgagggtgag ccggtcgagg agacccgcag acaggccgtc
     2461 ggcgcggccg cgggcgaggt cgtcgtggtt cgccgccacg atacgcccgg actcctcacc
     2521 gatccgccgg gctatcgcgg ccagcgcgtc gttcttcgtg ctggtggtcg ccgctgccag
     2581 ggctcgcgag gcgttcttcg cccgcgtcag ccggtcgacg atctcggcgg ggagtgtgcc
     2641 cgccgcgggg cccgggagag cggccgtggc cgcgtcggat atgacaggcg tggacatgca
     2701 cctattctat gaggcgccgg ccgccaggcc ctgttctcgg ccgagccgcg agccggtgtc
     2761 gcccgtgggc gccggtgcgg cgggcacgaa ccaggtgccg acctcccggc cggcgagcgc
     2821 ttctcgcacc tgcaccgcag aggtgaacag aaccgccgtg ccggcttcgg ccgcatgcaa
     2881 cgccgccgcc accttggtcg cagcaccccc ggtgccgacg ccggagccga cgccgccgat
     2941 ctccacgccg tcgagatcct ctgcacaccg gaccacgtcg atgcgtcgtg cgcccggctt
     3001 ggcgggcggc ctcgtgtaca gggcgtcaac gtcggaaagc agcacgagga ggtcggcgtg
     3061 caggagtgtc gccacgaacg aggcgagacg gtcgttgtcg ccgaaacgga tctcgtgtgt
     3121 ggcgaccgtg tcgttctcgt tcacgatcgg caggatgcgc agcgtgagca gccgctccat
     3181 cgcgcgccgc gcgttgcttc gggctgtcgg atcttgtagg tcgccggccg tcagcagcac
     3241 ctggccggcg aggatgccat aacgatcgag gctgtcttgg taccgataga tgagcacgtt
     3301 ctgcccgacc gcggcggcgg cctgctgcgt ggcaaggtcg atcggccgat cgtcgaggtg
     3361 caggtaaggc gtgcccgtcg cgatggcccc ggaggagacg agcacgacct cgccgccggc
     3421 cccgtatcgc tccgccaagg cgtcgacgag catgccgatc tgcccggcgt tggcaccgct
     3481 caccgacgag gagcccacct tgacgacgac ccgccgggcc cgggctgcgt cgtgccgctt
     3541 ctcgaggatc atgacgctcc cggccccgtg tcgggcgacg gctcgtcggt cgcctcggca
     3601 ggcgggaaga taccggcctt gcgctcttct tcgagctcgg cacgggcggc cgccttcgcg
     3661 tccatccacg tgaggtagtc ggcacggcgc tcccgatgcg tcgccctcgc ccgcccctcg
     3721 acccgctcat cggtgccgcg ggggcccgtc acgagctccg cggcggaggt cagggccggc
     3781 tcccagtcga agaccacgcc tgccccttgg ccgatgacga cggtcgatcc tgcgaccgcg
     3841 ccggcggcca gcagggcctc ttcgacgccg aggcgcagga gccggtcggc gaggtagccg
     3901 accgcctcgt cgttggcgaa atcggtctgc gcgacccaac gttcgggctt cacgccgcgc
     3961 acgcgaaaga cgggttccgc cccgccctcg cggaccacgg tgaagcccgg gtcgttcacc
     4021 ggccgaggcc gcaagaggat gcgtgccggc tcggggcgct catgcctgcc tcgttccgcc
     4081 tccacccgct ccgcgatcgc gaacgagagg gcccggatgc cttctctgct caccgccgag
     4141 acctcgaaga ccggcaaccc gcgctcctcg atcatgccgc gcacgaactg cgcgagctcg
     4201 cgggcctcgg gaacgtcgat cttgttcaag acgacgatct gcggccgctg gcccaatggg
     4261 gtgcgccctt cggcgaccgg ataggtggcg agctcccgca ggatgacgtc gaggtcgctc
     4321 agcgggtcac ggccctcctc cggcgtcgcg cagtccacca catgaacgag cacgctgcag
     4381 cgttccacat gccgcaagaa ctcgaggccg aggcccttgc ctgcgctcgc gccctcgatg
     4441 aggccgggca cgtcggcgat cgtgtagcgg ctctgcccgg cctggaccac gccgagatgc
     4501 ggtcgcaagg tcgtaaaagg gtaatccgcg accttggggc gcgcggccga cagggcggcg
     4561 atgaggctcg acttgccggc ggaagggaac ccgacaaggg ccgcatcggc gagcgtcttc
     4621 agctccaaca ggatgtcgcc cgaccagccg ggcgtgccga gaagcgcgaa cccgggtgcc
     4681 ttgcgtttcg tcgtcgcgag ggcggcgttg ccgagcccgc cgatgccgcc ctcggccacg
     4741 acgacccgca tgcccggctc cgtgaggtcg gccagaaggg tgccgtcggc ggttcggacg
     4801 acggtgccga ccggcaccgg cagaacgagg tcttccccac gctgcccgtg ccgaccgccg
     4861 cccatgccga accctccgga gtcgctggcc cggtgcggcc ggcgccggta gtcgagcaac
     4921 gtcgtcacct gcgattcggc ggcgagaacg atgtcaccgc cgtgcccgcc gttgcccccg
     4981 tcaggaccgg ccaggggctt gtacttctcg cgccggatgg agacgcaacc gtcgccgccg
     5041 tctcctgcac tcacgtgcag ggtcaccgtg tcgacgaaat gcgccatgac cctcccgccg
     5101 gcctccggtg atgataagcg cggggcggac cgaagcccgc cccgcgcgcg attgctctac
     5161 gatccgcgtt caggcggaaa cgatgttgac caccttgcgg cggcccttct gcccgaactc
     5221 cacctcaccg gctgagagcg cgaacaacgt gtcgtcgccg ccgcggccga cgttcacgcc
     5281 ggggtggaaa tgcgtgccgc gctggcgaac gaggatctcg cccgcgttga cgctctggcc
     5341 ggcgaaccgc ttgacgccga gccgctgcgc gttggaatcg cgaccgttgc ggctggagct
     5401 cgcccctttt ttgtgtgcca tgacaatgcc ctttccgcgg gactacttga tgcccgtgac
     5461 cttgacgcgc gtgagctcct ggcggtggcc ctggcgcttg cggtaaccag tcttgttctt
     5521 gaacttctgg atgacgatct tcttgccgcg gaggttctcg aggacttcgg cggtcaccgt
     5581 gacgttcttc agcaccgtgc ggtcggaggt gaccgtgtcg ccgtcgacga gcagaacggc
     5641 aggcagacgc acggtgccac tctcgtcggc ctcgacgcga ttcatcgtca cgacggtgcc
     5701 gacctcgact ttttcttgtc gcccgccggc gcgcactact gcatagacca cttcgtacct
     5761 gttctctcgg ggtctcaccc acacgatcga gaccctcact caattcggtt gacgccatcg
     5821 gggtgccttc gcacctcgaa ctcatctctc ggcctcgccg gacgaccccg agcctcgaca
     5881 cagaaggccg acggcctgcg tgacccggcc tgggacacca cgcggacatg ccccgccatg
     5941 acgcgccagc ggcctacttt accggaaagc gatccggctg gtcaatttag gcgccgctct
     6001 agactcatgg cgtgaccgtt ctcatcgacc gcccgctgtg gccggcacac ggcacggtct
     6061 tctcccacct cgtcagcgac gcctccctgc acgaactgca cacgttcgcc acacggcacg
     6121 gcatcccccg ccgcgcgttc gatctcgacc attacgacgt gccggtgtcg acgtacgccg
     6181 agctggtgtc ggccggagcc ctcgaggtca ccgggcggca actcgtctta cgccttgccg
     6241 ccagcggcct gcgcgtcaga ggccgagacc ggccgtcgcg tgcgcgtgcc ggcgcctcac
     6301 tcgtcgagcg acccgtcgcc cgccgcctcc acatgcaatg acccggtggt gacccgccgc
     6361 ctcgtgcggg gcggcgtcgc cttcttcggc tgcggcaacg cgtcgagcac cgagtcgagc
     6421 agatgctccg cctcggaggt gctgacccgc tgccggcgcc ggctcggggc gatcgggata
     6481 tcgagaatgg cgacggcctg ctcgctttcg tgccgcgccc cgcccgtctc gttctctgcg
     6541 atctggtcct ccgccgtgcc cgacgggtgg ctgagggtgc tcgccgcgat ctgtgccagc
     6601 cccttcttgg ccttctccgt caaggcgtgc gtcgtcttcg cggcggcctg ccgcgacgag
     6661 gccttgcgtt tcgtctgagc cggcaccttg tgcttcaaca cgggatcgtg gtgcacgacg
     6721 acgccgcggc ccgcgcagac ctcgcacggc tcgccgaagg tctcgagcag gccgaggccg
     6781 atcttcttgc gcgtcatctg gatgagcccc agcgaggtca cctcggccac ctgatgcttg
     6841 gtgcgatccc ggctcaggca ttcgacgagg cggcgcagca cgaggtcgcg gttcgactcc
     6901 aggaccatgt cgatgaagtc gacgacgatg atcccgccga tgtcgcgcag ccgcagctgc
     6961 cgcacgatct cctcggccgc ctcgaggttg ttcttcgtga ccgtctcttc gaggtttcca
     7021 ccggagccga cgaacttgcc cgtgttgacg tctatgacgg tcatcgcctc ggtgcggtcg
     7081 atgatgagcg tgccgccgga gggcagccag accttgcgct ccagcgcctt ctcgatctgc
     7141 tccgcgatgc ggtagtggtc gaacgagtcg tcgtcaccgt cgtatcgctc gaccttctcc
     7201 acgaggtcgg gcgccacctg agaaaggtaa tgctcgatgg tggctcgcgc ctcatcgcca
     7261 tccacgacaa gacggtggaa gtcctcgttg aaaatgtcgc gcacgatctt gatgagcaga
     7321 tcgggctcgc tgtgcaagag cagcggggcc tggccgcctt gcacctgcgc gctgatcgcg
     7381 tcccactgcc cgcgcaggcg atccacgtcg cgggtgagct gatcgtccgt ggcaccttcc
     7441 gccgcggtac gcacgatcac gccggcgtct tcgggcaaca gcgccttcag aatcttcttc
     7501 agccgggccc gctccgtgtc gggaagcttg cggctgatgc cgttcatcga gccgcccggc
     7561 acgtagacca gatagcggcc gggcagggag acctgactgg tcagccgtgc ccccttgtgg
     7621 ccgacggggt ctttcgtcac ctgcacgaga accgtgtcgc ccggcttcaa cgcgttctcg
     7681 atgcggtgtg ccttatcgga gccgaggcgc tccgcggccg cctgccagtc gacctcgccg
     7741 gagtagagca cggcgttgcg accccgcccg atgtcgacga acgcggcctc catgctgggc
     7801 agcacgttct gcacgcgccc caggtagacg ttgccgatca acgatgcctc ttgggagcgc
     7861 gcgacatagt gctcgacgag cacgccgtct tcgagcacgc cgatctggat gcggtcttgt
     7921 ctctctcgca cgaccatggc gcgatcgacg gattcgcgcc gggcgagaaa ctccacctcc
     7981 gtgatcacct ggcgacggtg gctgtcggcg cggccgtcgc ggcggcgctg ctttttcgcc
     8041 tcgagacggg tcgagccctt gatcttctgc ggctcggtga tgagcgggcg cgtctgccga
     8101 tcggtgtcac gacggccggg ctggggccgg tcgccgtcgc ggtcgtcacg gcccgaacgg
     8161 tgcctggagc ggcgtcggtg ggaggtctcg ggctcctcca gatcgccgtc ggcctcgtgc
     8221 cagccgggca gcacttcggg agccatgaaa aggatcgtcg ccggcggcag gccgtcgggg
     8281 cgagtcacct ggggcaggag ggcatccgag ccccgcgcgt caccctgcgc cgcgtcggcc
     8341 ccgcccgtgt cattcgtgcc cgtgtcgttc gtctccacaa cgtcaccgtc cgcatcgctc
     8401 gcgctcgcgg cccccgcttg catgccgtca gccgtcggat cgttcgtttg aggcccgccc
     8461 ggcacgtcgg cggctgctcg gcccccctcg gccgccgcgt cgccccgtgc ccgccccaca
     8521 ccggcgccgc ttctcgcgac gctatcctca ccctcgacgg ccgcctggcg cgtctcgccg
     8581 tcgtccggtg cggcctcggc ctgtctcgtg cccggccgcc ccgtggcagg ttccgagtct
     8641 cggcccgggc tcgccacgct cacgcgccgt gcagtgcgcc tggccgaacg gtgccccccg
     8701 aagagcctgc cgaagcccct gggctccccc gccccgttgt tcgtctcgtc tgtcgtgttg
     8761 cggtctttgt tcttctccac catctctggt gcactccccg accggagggg ccgcgccccg
     8821 gctccggttc ctcttctctg cgcgccggcc tgcatgtgcc gcgcgtcgcg tcgtgcggcg
     8881 ggctggtggg cccgggccgc agcgcatcat cgacgtcttc ttcggccggc gtgcgccggt
     8941 cgacacggga atcaacccgt caagccttct cggagtccga ccgggcgcgg cccgacggac
     9001 tggcatcatt atcgcacgcg atcgaggcgc agaccgacgg gcggctcatg tcgctgtgcc
     9061 ataatccgag ggtgcccgag cccggaaccg tcaagaaacc gaccgccctc gccatctttc
     9121 tcatcgtggc cggcacgatc ggactggcgg cggcgttcct gctcacgaac gacaagttca
     9181 ccctgttgca aaacccccac gcacaactca actgcaactt cagcgtgctg gtcggctgct
     9241 ccaagaacct cacggaatgg caaggctcgg tgttcgggtt cccgaacccg gtcctaggcc
     9301 tcatgtgttg gccggtgccg ataacggtcg gggtggccac cttggccgga gcccgactgc
     9361 cacgctggtt ctggctgctt ttcaacctgt tcgtcttggc cgcggtcgtg ttggtcgggt
     9421 ggttcatctg gcagagcgtg ttcgccctgt tcgtcctgtg cccgtggtgc atggtgacgt
     9481 gggcggtgac gatccccacg ttctgggcct tgacgcttca caacctctcg tcggggcaca
     9541 tcccgctcgc gcctcgggcg cgccgcgtgt tccataccct gtacagctgg acacccgtca
     9601 tcgtgttgct cagctacgca ttcgtcgccg tgcttgccca gatccaactc gactggctgc
     9661 atcggctctg acaccggcac aggcgggccg ccgaccgagc tgaccgggcg cggcgcccgc
     9721 ccgccccgct cagaaccaga gggcgagctc ccgtgcggca gaggccggcg agtcgctgcc
     9781 atgcacgaga ttctgttgca cggcgaggcc ccagtcacgc ccgaggtcgc cacggatcgt
     9841 gcccggtgcg gcggccgtgg gatcggtggc accggccagc gagcggaacc ctgcgatcgc
     9901 acggtggcct cgtacgcgcg ccgcgacgat cgggcccgag agcatgaact cgacgagcgg
     9961 ctcgtagaac ggcttgccga catgctcggc atagtggcgg gcgaggaggt ctctgtcggc
    10021 caggcgcatc gtcaggcccg tgatgcggta acccttcgcc tcgatgcggc gcaagatctc
    10081 gccggtgagc ccgcgcgcca cgccgtctgg cttcacgagc acaagggttt cctcttcatc
    10141 gttgcccata ggttgttcct ccaaagatga tcgctatgcg gatgggtgaa gacagcgcgt
    10201 cgcgggcgct catgcctcgg cgcgatccaa gcggataccg gtgaccatgc accaggtcca
    10261 aatcgcggtg aagatgatgc cgaccacgaa gagcatgccg atcaaaaaac cgcctgcgac
    10321 caccgcgagt tgggtgagcc agccgagaac gtacgcgatg cgatgcggca gcaacacgag
    10381 cgcgacaagc atgacgagga tcacggcgcc cccgacgaga aaggcctgcg ccggcggcag
    10441 agccttcagg ccgaagacga cgagcatgcc caggaagacg acgacgacct cgaaccccag
    10501 cacgatctgg gcgaggctgt gttgggccga gcggggcttc gccggccgcc gacgccccct
    10561 cacgcgcgga cccccgtctc ccgcaaccag ccatgctcgg cagcgacacc caacgcgtcg
    10621 ccgacgagtg cgatcgaccc cgtgacgaga accgcgcggc ccgaggcagc gcccgcccag
    10681 gcgcgtgccg cctcgagcgc gtcggcgacg tcgccggcgt cgtggacacg ttggtcgccc
    10741 aggcggctcg cgagttttct ggccagatcg gaatgcccga gtgccctgga cgagtgggat
    10801 tgtgaggcga agaagacgct gccgtgcggc cccacgtggt cgaggatgcc gggaaggtct
    10861 ttgtcggcca tcacaccgaa cacgaccgcg atctccccca ggtggaagta gtcgtcgagt
    10921 gccgccgcca ggctcgcggc gccaccggga ttgtgtgcgg catcgacgag caccgtcggg
    10981 tcggcgccga tgacctggag tcggccggg
//
LOCUS       DASTNL010000243         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_183073, whole genome shotgun sequence.
ACCESSION   DASTNL010000243 DASTNL010000000
VERSION     DASTNL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183073"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFQ96_07880"
     CDS             complement(<1..191)
                     /locus_tag="VFQ96_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0257743.1"
                     /translation="MGISRLIIFDCDGVLADTERAGHLPAFNRTFEEFGVPIHWSDAE
                     YAQRVKIGGGKERMLSVLT"
     gene            complement(198..1058)
                     /locus_tag="VFQ96_07885"
     CDS             complement(198..1058)
                     /locus_tag="VFQ96_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU0257744.1"
                     /translation="MPVVSLKEIVTRSFEGHYGVPAINIVNDLTLEAVLAGAVEARSP
                     LIVQTSVKTVRSIGADVLFGMWRTMTRGIEVPVTLHLDHCPDRTVISECLRRGWNSVL
                     FDASTMPVEENLRQTVEVVAEAREYGAHVEGEIEAITGVEDGIGSDTVAHRESLETAL
                     HFIRTTGVDVFAPSIGNAHGVYKTTPTLDTQRVSDITQATGVPVALHGGSGMTDEQFT
                     ELISRGCAKVNISTALKITFMKSSLEFLKEAEERDKWDPPSLFRHVRESVVALTRGLA
                     TTFGSAGKAW"
     gene            complement(1124..2248)
                     /locus_tag="VFQ96_07890"
     CDS             complement(1124..2248)
                     /locus_tag="VFQ96_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU0257745.1"
                     /translation="MPAPPDFEVGRRLRPVAKVLPARARSHNRALVLQTLYHGTGLSR
                     ADLARATGLTRVTISDLVAELLDDGLVVEKGPRDEARPGKPAVLLDINHDVAQIIGID
                     LSEDVVFRGAVLGIDGQVVERAQTALDGAVGEEALGKVLSLVDRLRGRSTRRLLGIGI
                     GTPGVVTPEGLVDSAPNLGWTRLQLQETVTSYTGTRSYVGNDANVAALAEYGFGPADG
                     DMIMVKLGHGVGAGLIVSGSLVWGSRYAAGEIGQITTVGEANGTAKALETVLAVPRLR
                     EALSVPGASGDGVLEDAGRRLGEALVPVVGALNPAEVVLSGPEDLVEGVFLAAVLDTL
                     RRQTMNGFHTGVTVRMTRLGADIVLRGAAVLVLSRELGVS"
     gene            complement(2309..>4045)
                     /locus_tag="VFQ96_07895"
     CDS             complement(2309..>4045)
                     /locus_tag="VFQ96_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU0257746.1"
                     /translation="GYGVVAAYGNQRTPDGQPVFFESGIPTHGSLGAYQPRIYFGESS
                     PSYSIVGGTKSASNPQGIELDYPAGSAKEGGETYTSYTGNGGPKLNNVFNRLVYALKF
                     QSEQILLSSSVNNQSQILYDRNPAKRVKDVAPYLTIDSDPYPAVINGQVEWIIDGYTT
                     SDNYPYSQEQSLSQTISDVSTPSAYAPLDDVNYMRNSVKATVNAYTGKVTLYAWDPGD
                     PILKTWEKVFPGTIKPAKDMSPQLMDHVRYPTDLFKMQRAVLGEYHVTDPGSFFSRED
                     AWVTPPDPVSSTTSSASLAGTTQLQPPYYLTLQMPGQKKPAFSLYSTYIPQATESSTR
                     SVLRGYLAADGDAGGTPGKIAAGYGKLRLLGLPTSDNVPGPGQVENTFSSDPTVSQQL
                     NVLRLGKTDVISGNLLTLPVGGGLLYVQPIYVKSTGETSYPLLQKVLVAFGDNVAFED
                     TLDGALNKLFGGNSGASAGDTGVVGKTGGTSEAPSTGSSSGTSGSSGTSGSGATGGGS
                     GQAAGGTTLTDALEAAKQAMIARNDALKKGDWAAYGKADSQLTQAIDQALAADGAIGT
                     GSSSGSTSSNGQ"
BASE COUNT          630 a         1362 c         1353 g          700 t
ORIGIN      
        1 tttgtcaaga cgctcagcat gcgctccttg ccgccgccga tcttcacccg ctgcgcgtat
       61 tcggcgtcgc tccagtgaat cggcacgccg aactcctcga aggtgcgatt gaacgccggc
      121 aggtggccgg cccgttcggt gtcggccaac acgccgtcac agtcgaagat gatgaggcgc
      181 gagataccca cgacgcgtca ccacgcctta ccggcgctgc cgaacgtggt tgcgagccct
      241 cgcgtcagag ccacgaccga ttccctcacg tgacgaaaga gcgagggcgg atcccatttg
      301 tcgcgctctt cggcctcttt caagaactca aggctcgact tcatgaacgt gatcttcaac
      361 gccgtcgaga tgttcacctt cgcgcagccg cgcgagatga gctcggtgaa ctgttcgtcg
      421 gtcatgccgc tgccgccgtg cagggcgacc ggaacgccgg tggcctgcgt gatgtcgctg
      481 acccgctgcg tgtcgagcgt cggggtcgtc ttgtagacgc cgtgcgcgtt gccgatcgaa
      541 ggtgcgaaga cgtcgacccc cgtcgtgcgg atgaaatgca acgccgtctc cagcgactcc
      601 cgatgcgcga ccgtgtcgga gccgatgccg tcttcgacgc cggtgatcgc ctcgatctca
      661 ccctcgacgt gcgccccgta ctcacgcgcc tcggcaacca cctcgacggt ctgccgcagg
      721 ttctcctcca caggcatggt cgaggcgtcg aaaagcaccg agttccagcc ccgccgaagg
      781 cactcgctga tgaccgtgcg gtccgggcag tgatccaggt gcagcgtgac cggcacctcg
      841 atgccgcgcg tcatcgtgcg ccacatgccg aagagcacgt cggcgccgat ggagcgcact
      901 gtcttgacgg aggtctggac gatgagcggc gagcgagcct cgacggcgcc cgcgagaacc
      961 gcctccagag tcagatcatt gacgatgttg atcgccggaa ccccgtagtg gccttcgaac
     1021 gatcgggtga cgatctcttt gagtgagacg acgggcatcg tgacctcctt cggccatccg
     1081 ttgtcaggtt atgccgaccg ccgacctgcg acaagtggcg ccgtcaggaa acacctagct
     1141 cccgcgaaag caccagaacc gccgcaccgc gcagcacgat gtccgcaccg agcctcgtca
     1201 tacgcaccgt cacgcccgta tggaagccat tcatcgtctg gcgccgaagg gtgtcgagga
     1261 ccgcggccag aaagactcct tcgaccaggt cttccggccc gctcaagacc acctccgccg
     1321 ggttcagggc accgacgacg ggtacgagag cctcgccgag ccggcgcccc gcgtcttcga
     1381 gcactccgtc gcccgaggca cccggcaccg acaaggcctc ccgcaggcgc ggcaccgcga
     1441 ggaccgtctc cagagccttc gctgtgccgt tcgcctcgcc caccgtggtg atctgcccga
     1501 tctcgcccgc cgcataccgg ctgccccaca cgagcgagcc cgagacgatg agcccggcgc
     1561 cgactccgtg gccgagcttc accatgatca tgtcgccatc ggccgggccg aagccgtatt
     1621 cggccagggc ggcgacgttc gcgtcgttgc ccacgtagct gcgagtgccc gtgtagctcg
     1681 tgacggtctc ttgcagctga aggcgcgtcc accccaggtt gggcgccgag tcgacgaggc
     1741 cctccggcgt gacgacgccg ggcgtgccga tgccgatgcc aagaagcctc ctcgtcgacc
     1801 ggccgcgcaa ccggtcgacg agggagagta ccttgccgag ggcctcttca ccgaccgcgc
     1861 cgtccagcgc cgtctgtgcg cgttcgacga cctgcccgtc gatgccgagc acggcaccgc
     1921 ggaagacgac gtcctcactg aggtcgatgc cgatgatctg ggccacgtca tggttgatgt
     1981 cgaggaggac ggcaggcttg cccggccggg cctcgtcacg aggccccttc tcgacgacca
     2041 ggccgtcgtc gagaagctcg gcgacgaggt cggagatcgt gaccctggtc agccccgtcg
     2101 cccgggcgag atccgcccgg ctgaggccgg tgccgtggta cagggtctgg agcacgagcg
     2161 cccggttgtg agaccgggcc cgcgcgggga gcaccttcgc gaccgggcgc aggcggcggc
     2221 ccacctcgaa gtcgggtggg gccggcaacg ccctcggcgg gccccccgga cccgcgctac
     2281 ggcgtggcgt catctcggcc ggccctggtc actggccgtt ggaagacgtg ctgcccgagg
     2341 agctgcccgt gccgatcgcg ccgtctgccg ccagggcctg gtcgatcgcc tgcgtcagct
     2401 ggctgtcggc cttgccgtac gccgcccaat cgcccttctt caacgcgtcg ttacgggcga
     2461 tcatcgcctg cttggccgcc tccaaggcgt cggtgagcgt cgtgccgccc gcagcctggc
     2521 cggaaccgcc gcccgtcgca ccgctgcccg aggtgcccga cgaacccgag gtgcccgacg
     2581 aggatccggt cgagggggcc tcggaggttc cgccggtctt gccgacgacg ccggtgtccc
     2641 cggcactcgc ccccgagtta ccgccgaaca gcttgttcaa ggcgccgtcg agcgtgtctt
     2701 cgaaggcgac attgtcgcca aacgcgacga gtaccttctg caggagcggg tagctggtct
     2761 cccccgtcga cttgacgtaa atcggctgca cgtagagcag gccgccaccg accggcagcg
     2821 tcagaaggtt gccgctgatg acgtcggtct taccgagccg gagcacgttc agctgctgcg
     2881 acaccgtcgg gtcggagctg aaggtgttct cgacctgccc gggcccgggc acgttgtcgc
     2941 tcgtcggcag cccgagaagg cgcagtttgc cgtagcccgc ggcaatcttg cccggcgttc
     3001 cccccgcgtc gccgtctgcg gcgaggtagc cgcgcaacac gctgcgggtg gaggattcgg
     3061 tggcctgcgg gatgtacgtc gagtacagcg agaaggcggg cttcttctgc cccggcatct
     3121 gcagggtcag gtagtaaggg ggctgcagct gcgtcgtacc ggcgaggctc gcggacgagg
     3181 tcgtcgacga taccgggtcg ggcggggtca cccatgcgtc ctcgcgcgag aagaacgatc
     3241 ccgggtctgt gacgtggtat tcgcccagca cggcacgctg catcttgaag aggtccgtcg
     3301 ggtagcgcac gtggtccatg agctgcgggc tcatgtcttt ggccggcttg atggtgccgg
     3361 ggaagacctt ctcccacgtc ttcaagatgg ggtcgccagg gtcccacgcg tagagcgtca
     3421 ccttgccggt gtaggcgttg accgtcgcct tgacggagtt gcgcatatag ttcacgtcgt
     3481 cgagtggcgc gtatgcggag ggcgtcgaga cgtcgctgat tgtctggctg aggctctgtt
     3541 cctgcgaata agggtagttg tcgctcgtcg tgtacccgtc gatgatccac tcgacctggc
     3601 cgttgatcac ggccgggtac gggtcggaat cgatggtgag atacggcgcg acgtctttga
     3661 cccgcttcgc cgggttgcgg tcgtacaaga tctgcgactg gttattcacc gaactggaaa
     3721 gcaggatctg ctcgctttgg aacttcagcg cgtagacgag cctgttgaag acgttgttga
     3781 gcttcgggcc gccgttgccc gtgtaggagg tgtaggtctc accgccctcc ttcgcgctgc
     3841 cggccgggta gtcgagttcg atgccctgcg ggttggacgc cgacttggtg ccgcccacga
     3901 tggaatacga cggcgagctc tcgccgaagt agatgcgcgg ctgataagcg ccgagggagc
     3961 cgtgcgtcgg gatgccggac tcgaagaaca ccggttggcc gtcgggcgtg cgctggttgc
     4021 cgtatgccgc gacaacgccg tagcc
//
LOCUS       DASTNL010000244         9042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_552810, whole genome shotgun sequence.
ACCESSION   DASTNL010000244 DASTNL010000000
VERSION     DASTNL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552810"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFQ96_07900"
     CDS             <1..268
                     /locus_tag="VFQ96_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257747.1"
                     /translation="LIWCGTAVSELPPALRGGIRALDPCEDGTILVATNDNGIQTAPY
                     TVDLARASTRVGQATRLAGEARYVQKIAMPGWRLYDSVSQTLTA"
     gene            387..776
                     /locus_tag="VFQ96_07905"
     CDS             387..776
                     /locus_tag="VFQ96_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257748.1"
                     /translation="MTSATAYRPGVCNIGPAEIRKRRASGYVGLAVTVVFLVVAFALR
                     WPAPWRLFAVLPAFLAAQGFLQAAFHFCVGFATRGLYNFGRLGAVESVREAGFRKQDQ
                     REALRVSALAFVLAVLVGLIALVIPVS"
     gene            complement(828..1607)
                     /gene="pstB"
                     /locus_tag="VFQ96_07910"
     CDS             complement(828..1607)
                     /gene="pstB"
                     /locus_tag="VFQ96_07910"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU0257749.1"
                     /translation="MPTRITVTDLEASYGDVAAITGVSMAIEPCSVTALIGPSGCGKS
                     TFLRTLNRMHEVTPGGRVTGRVLLDGDDLYAPGVDPVLVRRRIGMVFQRPNPFPTLSI
                     RDNVLAGVRLASRHMPRGEQDDLAEEALRAANLWEQVKDRLSASAAGLSGGQQQRLCI
                     ARAIAVQPDVLLMDEPCSALDPIATLAIEELIVRLKQEYTVVIVTHNMQQAARVSDRT
                     AFFTIAGTGEAGRLIEYDETQTIFTHPTQDATADYIAGRFG"
     gene            complement(1674..2849)
                     /gene="pstA"
                     /locus_tag="VFQ96_07915"
     CDS             complement(1674..2849)
                     /gene="pstA"
                     /locus_tag="VFQ96_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU0257750.1"
                     /translation="MITAIAETPHQAPHRDVRPQAPARLTARTLPYAARPSIVAAATV
                     LGAAAVFVGGASIAGGLALAAVAGTAGVVVASLVVEGRRRATDRMVHALMTAAFVLAV
                     IPLVSLLATVVNKGWASLSWQFITQTGGTAFDPTTLKVLTTAGAWQAVVGTLIITGIA
                     VALSVPIGVLTAIYLVEYANPRGWMARTTTFLVDVMTGIPSIVAGLFAFSLASLLVGP
                     KAFSGFSASVALSVLMIPIVVRSTEEMLRIVPDDLREASLALGATKVVTTLRIVLPTA
                     ATGILTGVMLAVSRIVGETAPIFIAASFTDNFSADPFHGPMQTLPVMAYANYKFPGTD
                     IAASYQSAWGAALLLVLLVVALNLAARLTARLVSGARRGRSSARRTPAEASMRDTHP"
     gene            complement(2846..3841)
                     /gene="pstC"
                     /locus_tag="VFQ96_07920"
     CDS             complement(2846..3841)
                     /gene="pstC"
                     /locus_tag="VFQ96_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU0257751.1"
                     /translation="MTAPHRVPRPGEGRPEPGRRRAGDTVFRWLTTGSAASIIAILAG
                     VTVFLIVTALPALTADWRTAPALAGGTTGGVGSFWRYVAPLLWGTAWSSLLALILGTP
                     VALGIALYVSHYAPRPLRRGLGYLIDLLAAVPSVVFGLWGIFVLRPAILPLQDWLNHN
                     LSWIPLFAGPVSKTGSTMLAAAIILAVMIIPIISSIAREIFAQTPRLHEEAALALGAT
                     RWEMIRLAVLPQARRGIVSATLLGLGRALGETMAVALILSPSLLFSVKILTDTANPQT
                     VAANLALNFPESTPLQRDALIATGLVLFIISFAVNALGRLVAGRAETGRRKRGAA"
     gene            complement(3856..4989)
                     /locus_tag="VFQ96_07925"
     CDS             complement(3856..4989)
                     /locus_tag="VFQ96_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU0257752.1"
                     /translation="MRLTMMPGAVGAVTLATALALTGCSAGAGAAGGAGTQAAHSTGD
                     TAATTSTLGGTITAGGSSAQANAQAAWTAAFQAQAHGVTINYDKSQGSGGGVVNWLNG
                     SYDFAGTDAGLTPAQQKQSQSMCGAGGALNLPVYLSGVAVVYHLPGVTLRLTSATVAK
                     IFTKKITRWNDPAIAADNPGQKLPDESITVVSRSDASGTTANFTNYLHQVQPKLWPYP
                     ASTTWPVKGTSGQQGGSGVVGTVLSGKGTIGYADHSSIGQAASAAIQVGSGSTFVGYS
                     PRGATRAFDTAARPAPQTSGDLFTDIDYTKITSANAYPIPLLSYDVVCTRLASPAQAK
                     LTAAYLSFVASDTGQKVAAKNAGSAPLPASIQRQIAHSLASVK"
     gene            complement(5235..5963)
                     /locus_tag="VFQ96_07930"
     CDS             complement(5235..5963)
                     /locus_tag="VFQ96_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0257753.1"
                     /translation="MTRLLVVEDEKAIAEPLGYLLEREGYTITSAADGPAALAAFRDR
                     GADLVLLDLMLPGMSGHDVCRTIRATSRVPIIMLTAKDSETDIVVGLELGADDYVTKP
                     YSSKELLARIAALLRRDAGGPGFPKEDAFEGGAGGAQARDPDTVAAHGIRLDVASHTV
                     LVEGRETPMPLKEFDLLELLLRNAGRVLTRGQLIDRVWGSDYHGDTKTLDVHIRRLRS
                     RVEPDPRSPCVITTVRGVGYRLNP"
     gene            complement(5960..7195)
                     /locus_tag="VFQ96_07935"
     CDS             complement(5960..7195)
                     /locus_tag="VFQ96_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0257754.1"
                     /translation="MGKRVAKAARRIVTALPTIGIIVDARGRVVKASKPARAARLIRH
                     RRLADERLAALVRQARAAGHTQELSVAVPSAVPGKTPAHLSVRAVPADGHGNVAILAD
                     DRSRQHRVEAMRRDFLANVGHELKTPISAVGLLAEALQAAYDDPARVKGFAARLQQES
                     ARLGRLVQDVLELSRLESTDADEAARPCRIDGVVAAAVDAVRVQAQAARVPLVVGAPT
                     GAWVFGDPRILATAISNLLSNAIRVSPQGEPVSVVTAVEHELVTVAVSDHGPGIAETE
                     LPRVFERFYRTDRSRSRDTGGTGLGLAIVKHAADTHGGDVRVTSSPGEGSTFTVRLPL
                     SDPDTTGDKDNDEDGAGNGGDTGAGGNDDEGDGAGAGDTGTGTGTGTGDTGTDADDGG
                     TGARGAKGAGANDHTEEAR"
     gene            complement(7459..7535)
                     /locus_tag="VFQ96_07940"
     tRNA            complement(7459..7535)
                     /locus_tag="VFQ96_07940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7499..7501),aa:Pro,seq:cgg)
     gene            complement(7757..8779)
                     /locus_tag="VFQ96_07945"
     CDS             complement(7757..8779)
                     /locus_tag="VFQ96_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0257755.1"
                     /translation="MASAEPMRLLPPQDRLPYPSRLTPRRARAGGVALGAALAGLAWG
                     GIVERNLFTLRRVTVRALPPGATPFTVLHISDLHMAPWQHRKQAWLRGLAALHPDLIV
                     DTGDNLGHREGLTGLRRALEAFRGTPGVYVNGSNDYFAPHPHNPLRYFTGPSRHSKTA
                     ERLDTASMERYFDDLGWVGLDNTAAGLDVRGTRVEFLGVDDPHRGFDRLELVVGALDQ
                     LREETAFDDDEEAERDLPAITIGVTHAPYRRVLNSFVTHGAQAIFAGHTHGGQICLPF
                     FGALVTNCDLPRRQAKGLSLWRNGFRSAYLNVSAGVGTSIYAPVRFACRPEASLVTFE
                     PGERTR"
     gene            complement(8766..>9042)
                     /locus_tag="VFQ96_07950"
     CDS             complement(8766..>9042)
                     /locus_tag="VFQ96_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU0257756.1"
                     /translation="PDAGQVIGQTLAQATQALAGFTVTTVGSDDPAGVVTAMSPAPGT
                     RIAASSTVTLTVEGAAAPLPSVTPPAGAGAGPEAPIVREGGARHGLR"
BASE COUNT         1260 a         2968 c         3407 g         1407 t
ORIGIN      
        1 gttgatctgg tgtggcacgg cggtgtcgga gctgccaccc gcgctgcgcg gcggcatccg
       61 ggcgctcgac ccgtgcgaag acggcaccat cctcgtcgcg acgaacgaca atggcatcca
      121 aaccgcgccg tacaccgtcg acctcgcccg tgcctcgacg cgagtcggcc aggcgacccg
      181 cctagcaggt gaggcgcgct acgtgcagaa gatcgcaatg cccggctggc gactttacga
      241 cagcgtgtcg cagacactga ccgcctagcc gtacgccctg cggagtggct cctccgtggc
      301 gacgagggcc acgtcgcgct cggcgcccga caggcaccga ggttggcgca cggcacggcg
      361 ccgtctcgat ggttcagact ggcattatga cgagcgcgac ggcatatcgg cccggggtgt
      421 gcaacatcgg gccggccgag attcgaaaac gacgggcgag cgggtatgtc ggcctcgcgg
      481 tcacggtcgt cttcctcgtc gtcgcgttcg ccttgcgttg gcccgctccc tggcgcctct
      541 ttgccgtgtt gcccgcgttc cttgcggcgc aggggttcct gcaggcggcc ttccacttct
      601 gcgtgggctt cgcgacccgc ggcctctaca atttcggtcg actcggtgcc gtggagtcgg
      661 ttcgcgaggc cggctttcgt aagcaggatc agcgcgaggc gctgcgggtc agcgcgctgg
      721 ctttcgtctt ggccgtgctt gtcgggctca tcgcgttggt catcccggtc tcctgacacg
      781 cgaccggcta cgagcgcgga acccgagccg ggcgcacggc accagggtca cccgaagcgg
      841 ccggcgatgt agtcggcggt cgcatcctgg gtgggatgcg tgaagatcgt ctgggtctcg
      901 tcgtattcga tgagccgtcc ggcctcgccc gtgccggcga tcgtgaagaa ggccgtgcgg
      961 tcggagacgc gggcggcctg ctgcatgttg tgggtgacga tgacgaccgt gtattcctgc
     1021 ttcagccgaa cgatgagctc ttcgatcgcc agcgtcgcga tggggtcgag cgccgagcac
     1081 ggctcgtcca tgagcaacac gtcgggctgc acggcgatgg cgcgggcgat gcacagccgt
     1141 tgctgctgcc cgcccgacag gccggcggcg gaggctgaca ggcggtcttt gacctgctcc
     1201 cacaggtttg cggcgcgcag ggcttcctcg gcgaggtcgt cttgctcgcc gcgcggcatg
     1261 tggcggctgg ccaggcgcac gccggccagc acgttgtcgc ggatcgatag ggtcgggaac
     1321 gggttcggcc gttgaaagac catgccgatg cgccggcgca cgagcaccgg gtcgacgccg
     1381 ggcgcgtaga ggtcgtctcc gtcaagcaac acgcggccgg tgacgcggcc gccgggggtg
     1441 acctcgtgca tgcggttcag ggtgcgcagg aacgtcgact tgccgcagcc gctcgggccg
     1501 atgagggcgg tcacgctgca cggctcgatg gccatgctca cgccggttat ggccgccacg
     1561 tcgccatacg aggcctcgag gtcggtgacg gtgatgcggg tcggcatggg cttctccttc
     1621 gtcgtgtcgg ctggctggtc ggtcggctgt ggtcggtcgg ccgcgggtga tggctacggg
     1681 tgcgtgtcgc gcatcgacgc ttcggcgggt gtgcgccggg cgctggagcg gccgcgccgg
     1741 gcacccgaga cgagcctcgc cgtcaggcgc gcggcgagat tcaacgcgac gacgagcagc
     1801 accaacagca gggcggcccc ccacgccgac tggtacgagg cggcgatgtc ggtgcccgga
     1861 aacttgtagt tcgcgtaggc catgaccgga agggtctgca tggggccgtg gaacgggtct
     1921 gcgctgaagt tgtcggtgaa gctggcggcg atgaagatgg gtgcggtctc gccgacgatc
     1981 cgtgagacgg cgagcatcac gccggtgagg attccggtgg cggcggtggg caacacgatg
     2041 cgcagggtgg tgacgacctt cgtggcgccg agcgccagcg acgcctcgcg caggtcgtcg
     2101 ggcacgatgc gcagcatctc ttcggtggag cgcacgacga tggggatcat caaaaccgac
     2161 agggcgacgg aggccgagaa gccgctgaac gccttcggcc cgacgaggag gctcgccagc
     2221 gagaacgcga agagccccgc cacgatcgag gggatgccgg tcatcacgtc gacgaggaac
     2281 gtcgtcgttc gtgccatcca ccctcggggg ttcgcgtatt cgacgaggta gatcgccgtg
     2341 agcacgccga tcggaaccga gagcgccacg gcgatgcccg tgatgatcag ggttccgacg
     2401 acggcctgcc aggcgccggc cgtggtcaga accttcaggg tggtcgggtc gaacgccgtg
     2461 ccgccggtct gggtgatgaa ctgccatgac agtgacgccc agcccttgtt cacgaccgtc
     2521 gcgaggagcg acacgagtgg gatgacggcc agcacgaagg cggcggtcat gagcgcgtgc
     2581 accatccggt ccgtcgcgcg ccgacgcccc tcgacgacca gggaggcgac gacgacaccc
     2641 gctgtcccgg cgacggccgc cagggcgagc ccgcccgcga tgctggcccc gccgacgaaa
     2701 acggcggcgg cgccgagcac tgtggccgcc gcgacgatgg acggccgggc ggcgtagggc
     2761 agcgtgcgag cggtgaggcg ggccggagcc tgtggccgca cgtcacggtg gggggcctgg
     2821 tgtggggtct ccgcgatcgc cgtgatcatg ccgcgccgcg ctttcgtcgg ccggtttcgg
     2881 ctcgcccggc gacgagccgg ccgagggcgt tcacggcgaa cgagatgatg aagagcacga
     2941 gcccggtggc gatcaaggcg tcgcgttgca gcggggtcga ttcgggaaag ttcagcgcca
     3001 ggttcgcggc gacggtctgc gggttggccg tgtcggtcaa gatcttcacc gagaagagca
     3061 gcgacggcga caagatgagc gcgacggcca tggtctcgcc gagcgcccgg ccgaggccca
     3121 gcagggtggc gctgacgatg ccgcgtcgcg cctgcggcag cacggccagc cgaatcatct
     3181 cccaccgcgt cgcgcccaag gcgagagccg cctcctcatg caggcggggt gtctgcgcga
     3241 agatctcgcg ggcgatcgaa ctgatgatgg ggatgatcat caccgccaga atgatcgcgg
     3301 ccgcgagcat cgtcgagccg gtcttcgaga cgggcccggc gaagagcggg atccacgaga
     3361 ggttgtggtt cagccagtct tgcaacggca gaatggcggg gcgcagcacg aagatgcccc
     3421 acagcccgaa gacgaccgac ggcacggcgg ccagcaggtc gatgaggtag ccgaggccgc
     3481 gccggagcgg gcgcggcgcg taatgcgaga cgtagagcgc gatgccgagg gcgacgggcg
     3541 tgccgaggat gagggcgagg agcgacgacc aggcggttcc ccacaagagg ggcgccacgt
     3601 accgccagaa cgacccgacg ccgcccgtcg tgccgccggc cagcgcgggc gccgtgcgcc
     3661 agtcggcggt gagggcgggc agcgccgtga cgatgaggaa cacggtgacg ccggccagga
     3721 tcgcgatgat cgaggcggcc gacccggtcg tcaaccagcg gaagaccgtg tcgccggccc
     3781 gcctgcggcc gggctccggc cggccttcgc cgggccgtgg cacccggtgc ggcgccgtca
     3841 tggtaggggt ggtcgtcatt tcaccgacgc caggctgtgg gcgatctgtc gctggatgct
     3901 cgcgggcagc ggggcagagc cggcgttctt ggcggcgacc ttctgccccg tgtcgctcgc
     3961 cacgaacgac aggtaggcgg ccgtgagctt cgcctgcgcc gggctcgcga gcctcgtgca
     4021 gacgacgtcg tacgagagca gcggaatcgg gtaggcgtta gccgaggtga tcttcgtgta
     4081 gtcgatgtcg gtgaagaggt cgccggaggt ctgcggcgcg ggccgggccg ccgtgtcgaa
     4141 cgcgcgggtc gcgccgcggg gcgaatagcc gacgaaggtg ctcccggagc ccacctggat
     4201 ggcggcggag gccgcctggc cgatcgagct gtggtcggcg tagccgatgg tgcctttgcc
     4261 ggagagcacc gtgcccacga cgccggagcc gccttgctgg ccgctggtgc ctttcacggg
     4321 ccacgtcgtg cttgccgggt agggccatag tttcggctgc acctggtgca ggtagttcgt
     4381 gaagttcgcg gtggtgcccg aagcgtcgga gcgggagacg acggtgatcg actcgtccgg
     4441 cagcttctgg ccggggttgt cggcggcgat cgcggggtcg ttccagcgcg tgatcttctt
     4501 ggtgaagatc ttcgcgacgg tcgcgctcgt cagtcgcagc gtgacgccgg gcaggtggta
     4561 gacgaccgcc acgcccgaca ggtagacggg caggttgagg gcgccgcccg ccccgcacat
     4621 gctctgcgac tgcttctgtt gcgccggggt gagcccggcg tccgtgccgg cgaagtcgta
     4681 cgacccgttc agccagttga cgaccccgcc gcccgagccc tgtgacttgt cgtagttgat
     4741 ggtgacgccg tgcgcctggg cctggaacgc ggcggtccag gccgcctggg cgttggcctg
     4801 ggcgctggag ccaccggccg tgatcgtgcc gccgagtgtc gaagtcgtcg ccgcggtgtc
     4861 gccggtggag tgtgccgcct gggtgccggc gccgcctgcg gcgcccgcgc ccgcggagca
     4921 tcccgtaagg gccagcgccg tggccaacgt cacggcgccg acggcgccgg gcatcatggt
     4981 gagtctcaca gtgcgtcctt cgcgtgggag ggggcgcgag gcccgtgccc gggtctcacg
     5041 gggccgtctt ccgacctgtc tgcgacgtta atcgggcgtc gtgaacggct tatggcgcag
     5101 aggtgaacgg gcggtgaaca ggcgatggca atgtcgtgcg ggggcgagca atggtgaggg
     5161 agacgccggg gcgagggccg agcggtgtcg ctgacggggc aggcggggcg ggatcgggat
     5221 gccggtgccg gacgtcaggg gttcaagcgg tagcccacgc cgcgcacggt cgtgatgacg
     5281 cacggcgagc gagggtcggg ttcgacgcgg gagcggagcc ggcgaatgtg tacgtcgagc
     5341 gtcttcgtgt cgccatggta gtcgctgccc cacacccggt cgatcaactg tccgcgggtg
     5401 agcacccggc cggcgttgcg taagagcagt tcgagcaggt cgaactcctt cagcggcatg
     5461 ggcgtctcgc ggccctcgac gagcacggtg tgcgaggcga catccagccg gatgccgtgt
     5521 gcggccaccg tgtcggggtc gcgggcctgc gcaccgccgg ccccaccctc gaaggcatcc
     5581 tctttcggga accccgggcc gccggcatcg cgccgcaaca gcgccgcgat gcgtgccaga
     5641 agctctttgc tggaatacgg tttggtcacg tagtcgtcgg cgcccagctc aagcccgacg
     5701 acgatatcgg tctcggagtc tttcgccgtc agcatgatga tcggcacacg ggatgtggcg
     5761 cgaatcgtgc ggcacacgtc gtggcccgac atgccgggga gcatgaggtc gagaagcacg
     5821 aggtcggcgc cgcggtcgcg gaacgcggcc agtgcggccg gcccgtcggc ggccgacgtg
     5881 atcgtgtagc cctcgcgttc caagaggtag cccaacggct cggcgatcgc cttctcgtct
     5941 tcgacgacca gcagacgcgt catcgcgcct cctcggtgtg gtcgttcgcg cccgctccct
     6001 tggcgccgcg tgcgcccgta ccgccgtcgt ctgcgtccgt gccggtgtcg ccggtgccgg
     6061 tgccggtgcc ggtgccggtg tcgcctgcgc ccgcgccgtc accctcgtcg tcgttgccgc
     6121 ctgcgcccgt gtcgccaccg ttgcccgcgc cgtcctcgtc gttatccttg tcgccggtgg
     6181 tgtcggggtc ggagagtggc aggcgcacgg tgaaggtcga tccttcgccg ggtgacgagg
     6241 tgacacgcac gtcgccgccg tgcgtgtcgg cggcgtgctt gacgatggcg agacccagcc
     6301 cggtgcctcc ggtgtcgcgg gatcgcgaac ggtcggtgcg gtagaagcgc tcgaagacgc
     6361 gggggagttc tgtctcggcg atgccgggcc cgtgatcgct cacggcgacc gtcacgagct
     6421 cgtgttcgac ggccgtcacc acgctcacgg gttccccttg tggggagacg cggatcgcgt
     6481 tggagagcag gttcgaaatg gccgtcgcga ggatccgggg gtcgccgaac acccaggcgc
     6541 cggtcggcgc gcccacgacg aggggaaccc gcgcggcctg tgcctgcacc cgcacggcgt
     6601 cgaccgcggc ggccacgacg ccatcgatgc ggcacggccg ggcagcctcg tcggcatccg
     6661 tcgattctag gcgggagagt tcgagcacgt cttgcaccag ccggccgagc cgcgccgact
     6721 cctgctgcag ccgtgcggcg aagcccttca ctcgtgccgg gtcgtcgtag gcggcctgca
     6781 gcgcctccgc caagagaccc accgccgaga tcggggtctt cagctcgtgc ccgacattgg
     6841 ccaagaagtc gcgccgcatc gcctccaccc ggtgctgcct gctccggtcg tcggccagga
     6901 tcgccacgtt gccgtggccg tctgccggca cggcccgcac actcaggtgc gccggtgtct
     6961 tgccgggcac ggcgctaggc acggcgaccg acagctcctg ggtgtggccg gcggcgcgcg
     7021 cctgccgcac gagcgctgcg aggcgctcgt cggcgaggcg gcgatgcctg atgagccggg
     7081 cagccctcgc cggcttcgac gccttcacga cccgcccccg ggcgtcgacg atgatgccga
     7141 tcgtcggcaa cgccgtgacg atgcggcgcg cggccttcgc gactcgtttg cccacgtgct
     7201 ccctccaccg tcgtgttgtc gaaggtgctc cagccccggc ctcgacctgc ggctgaggcc
     7261 gcctgtggcc ggagccggcc cgcgtcttgc gtcgtgcgcg tcgtgtggcg agcccggcat
     7321 ccttctgctg cccgtcgtat ggccagggta gcgtcgcgta tgtcgtctga ttgcgaagac
     7381 tgcgggcggg ggcgcatcag aggtctgcgg gccggcggta tcgcagcgcc cgcctcgcgg
     7441 tcagtgcggc tgccgtactg gtcggggtga caggatttga acctgcggcc tcctcgtccc
     7501 gaacgaggcg cgctaccaag ctgcgccaca ccccgatgcc cgtctcacgc ggcggaccac
     7561 ctaagaatac acggtgcgcc gacccggccg tacccgccgc tgctgtgctg gccgatacgc
     7621 cttctggtgg gagtgccctc aagttggtgt gccttctgac gagtgcgcgc ctcggtgggt
     7681 gaatcctcgg gcgattgcgt ttttccggcg cgtgcgcgcc gtgtgcgcgc cgtgtgcctt
     7741 gagcacatgc gcccttttag cgggtgcgct cgccgggttc gaacgtgacg aggcttgcct
     7801 ccgggcggca ggcaaatcgc acgggcgcgt agatggaggt gccgacgccc gccgagacgt
     7861 tcaggtaggc ggagcgaaag ccgttgcgcc acaggctgag gcccttggcc tgccggcgcg
     7921 gcaggtcgca gttggtcacg agggcgccga agaacggcag gcagatctgg ccgccgtgcg
     7981 tatgccccgc gaagatcgcc tgcgcgccgt gcgtgacgaa cgagttcaag acgcgccggt
     8041 atggtgcgtg ggtgacgccg atcgtgatgg ccggcaggtc tcgctcggcc tcctcgtcgt
     8101 cgtcgaacgc ggtctcttcg cgcagctgat cgagcgcgcc cacgacgagc tcaagccggt
     8161 cgaatccgcg atgcgggtcg tccacaccaa gaaactcaac ccgcgtgccg cgcacgtcga
     8221 ggccggccgc cgtgttgtcg aggccgaccc agccgagatc gtcgaaatag cgctccatcg
     8281 aagcggtgtc gagccgttcg gcggtcttcg aatgccggga ggggccggtg aagtagcgca
     8341 gcgggttgtg cgggtgcggt gcgaaatagt cgttggagcc gttcacgtac acgccggggg
     8401 tgccgcggaa cgcctccaac gcccggcgca acccggtgag gccctcgcga tggccgaggt
     8461 tgtcgcccgt gtccacgatg aggtcggggt gtaaggcggc caagcctcgt agccaggctt
     8521 gcttgcggtg ctgccagggc gccatatgca gatcgcttat atgtagcacg gtgaacgggg
     8581 tggcgccggg cggcagcgcc cgcaccgtga cccgccgcag cgtgaagagg ttccgctcga
     8641 cgatcccgcc ccaggcgagg ccggccagag cggcgccgag cgcgacgcct cctgcccgtg
     8701 cgcgtcgcgg cgtgagcctc gaggggtacg gcagacggtc ctgtgggggg agcaatcgca
     8761 tcggctcagc ggaggccatg acgcgcgcct ccttcccgca cgatgggggc ttcgggcccc
     8821 gcccccgcgc cggccggtgg tgtcacactc ggcaggggcg ccgcggctcc ctcgacggtg
     8881 agcgtcaccg tcgaggaggc ggcgattctc gtgccgggtg ccggcgacat ggcggtgacg
     8941 acgcccgcag ggtcgtcgct accgacggtc gtgacggtga agccggcgag cgcctgcgtc
     9001 gcctgcgcca aggtctgccc gatgacctgg cccgcgtcgg gc
//
LOCUS       DASTNL010000245        15349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_923663, whole genome shotgun sequence.
ACCESSION   DASTNL010000245 DASTNL010000000
VERSION     DASTNL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923663"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFQ96_07955"
     CDS             complement(<1..993)
                     /locus_tag="VFQ96_07955"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0257757.1"
                     /translation="MSERNPLAPAWLVPGGDDVPGTLWPSGAGRDAAGRLTIGGLAAT
                     ELAERYGTPLYVVDEDEARGRARRILRAFGDAFSPLGAAVRVYYAAKAFLSVQSARWM
                     REAGLGIDVCTGEELAVALAAGVEPAWLGLHGNNKSPAEIDEAVRVGVGTIVVDSPIE
                     IERVAQAAARHGRVQAVRLRVNSGVHAHTHEFLATAHEDQKFGLSLDDAPAAVARIRS
                     LPGLSFLGLHGHIGSQIFGIEGFARAAERLVGLHARLLAGGPVPQLNLGGGFGIAYTA
                     ADRPAAIEDVAAGIARAVAGQCRALGVPVPDMAFEPGRWIIGPAGVTLYRVGTVK"
     gene            complement(990..2813)
                     /locus_tag="VFQ96_07960"
     CDS             complement(990..2813)
                     /locus_tag="VFQ96_07960"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU0257758.1"
                     /translation="MASAAATVTAAAMARAGERLASGRWVSRALNIVARLPTATPDEA
                     ATPLVESILVTSADLPATLHEIVSRVATRHGVSADRLPGPEGVVLERPKSREHGDWTT
                     NVALRLAKAFGLVPHAFAAEIAAELAAAAGVASAWPAGPGFVNIRLDAGVAGELARAI
                     VRAGERYGRGESEAGLRVNMEFVSANPTGPLHIGHTRWAALGDSIARVLRAAGAQVTS
                     EYYINDAGHQMDVFGESVLAAALGEPTPEGGYPGSYIDDLAAVVLEEEPGLATLPRVE
                     AVVLAREIAYRHQLAEIRESLAQFNVRFDVWFSERLLHTPDKEGKSAIERAVEHLRAA
                     GHVVEKDGAVWVRTTDFGDDKDRVIRRSNGVFTYFAADAAYYLDKSRRGFPHKIYLLG
                     ADHHGYIGRLKALAGAAGDDPEADIEVLIGQLVSVNGARLSKRAGNIIELDDLRAWLG
                     TDALRYSLARSPADTPLTLDPDQLRRRANDNPVFYVQYAHARTRSVDRHAAAAGVDRG
                     AFVPETLGEETEAALLSGLREFPRIVAQAARLREPHRVARYVEEIASLYHRWYDTCRV
                     IPLGDEPVTDVHRTRLWLNDATGQVIRNGLELLGVSAPERM"
     gene            complement(2883..3014)
                     /locus_tag="VFQ96_07965"
     CDS             complement(2883..3014)
                     /locus_tag="VFQ96_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257759.1"
                     /translation="MEIIELQATRYTLTGVACGVVVVGGGDDGVAAGVVGAAATWRA"
     gene            3004..3408
                     /locus_tag="VFQ96_07970"
     CDS             3004..3408
                     /locus_tag="VFQ96_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257760.1"
                     /translation="MISTAQLYAFNPNVSAQPGATPPAGSLVAQAVAAGGTACGWVYD
                     SGNQSIQFALATPGPKALASLKEKAAADSTPTSAYGDGPNVAGFFHVTGSRGEVQVFT
                     TGHWLVGLSSTFFEPGDAEQLVHDILANLHLG"
     gene            3509..3581
                     /locus_tag="VFQ96_07975"
     tRNA            3509..3581
                     /locus_tag="VFQ96_07975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3542..3544,aa:Arg,seq:ccg)
     gene            3634..4836
                     /locus_tag="VFQ96_07980"
     CDS             3634..4836
                     /locus_tag="VFQ96_07980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEU0257761.1"
                     /translation="MLLNIALILLLAGYLVHGYRAGLTWSLAGLAGLAAGAVASFLVA
                     PLLGQSAGASLWRVPLIVAVTVVFLGLGHAVGSACGRVLRRGVHKTPLRLLDRALGAA
                     VDVTVAALVLAMLSFGIGSLGIPALSAAVGSSPVLRTIDALTPESLQTGLARLKDLVT
                     KDTAGRVLDSLASPAKTPDPTTTTASPTVPNVDTDTALLRQAARSVVKITGLAAACRQ
                     TQSGSGFVAAPRRVLTNAHVVAGVTDPVVQGADGRSYTARVVFFDARHDVAILAVPRL
                     TAPTLALSPVLGPGQDAVFDGYPLGGPFRSSPAAVRGVGTAKIHDIYNATTVSMPIYS
                     LAADVEHGNSGGPLLAPDGRVAGVIFATDANGAAIGYALTPSVVDPVVEAAPRETTPV
                     GTGRCVPG"
     gene            4940..5410
                     /locus_tag="VFQ96_07985"
     CDS             4940..5410
                     /locus_tag="VFQ96_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257762.1"
                     /translation="MEKLVLASTGADADAVVAIERRHTELGTAAAMKTLHVLNMVPRP
                     YDVVRAARDDLVWWAYSRLQPYLEREADLFFPALPSSSEADDAARDARYRLTEIAAAV
                     TRLRTDDTAHDIAADAIALRSHLTAFFETQQHRILPYLAFSRRPIHELWQLVNT"
     gene            5424..5720
                     /locus_tag="VFQ96_07990"
     CDS             5424..5720
                     /locus_tag="VFQ96_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU0257763.1"
                     /translation="MEPNQLTVTHDDGTICSCAEPDDAFPPLDVRQVPHSIRHAIVLG
                     ALDAVKPGLGIVLIAPHDPRPLLAQIDARYQGRFTVEYLVEKPTEVHIRFTRTA"
     gene            5717..6178
                     /locus_tag="VFQ96_07995"
     CDS             5717..6178
                     /locus_tag="VFQ96_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257764.1"
                     /translation="MSARLTAPLAVMVGIGGAAIVAGGMVSAATGPLGWAKGSWASAY
                     LVLVAGAAQVAYGLLQRPLARRVPSARIVVWQIVLFNLGNALVLAGSLATIPVVVDVG
                     GAAIVAALVLFLAAVRRRAAAHPAAVWTYRGLTVLLLVSIPVGLTISTLQA"
     gene            6299..6661
                     /locus_tag="VFQ96_08000"
     CDS             6299..6661
                     /locus_tag="VFQ96_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HEU0257765.1"
                     /translation="MPASARGIWSALAYGGYLYASAVGLAWGALWSTGRVERRGGLVV
                     FTGLPRWAFRRGGSCVGSCYLTFDNVSDDVLEHERVHRGQWRRYGLLFPLLNLLAGSD
                     PHHNRFEIEAGLEKGGYL"
     gene            6658..7530
                     /locus_tag="VFQ96_08005"
     CDS             6658..7530
                     /locus_tag="VFQ96_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0257766.1"
                     /translation="MSDGTRRRPLVVVTGASSGIGRLAARKLAEAGARVALVGRDPRR
                     TSEAALEAGGTPFVADFDRLGDVRRLAQQLDELGPIDVLVNNAGGLVQHRELTVDGNE
                     RIFQANHLAPFLLTNLLLAARHRRGDHRPFRVVSTASSAHRYGRLDLHDLTFARRPWR
                     AGWAAYGAAKLATVLFVRELARRAAADGLAGEVTAFAVHPGLVATGFVRDIPWLRLVA
                     RLTGGHYALGVEAGAAPLVRLARQTPGALSGSYFDRMRAGRGLAARARGPAADRLARE
                     LWRVSARLTGLDER"
     gene            complement(7576..8994)
                     /locus_tag="VFQ96_08010"
     CDS             complement(7576..8994)
                     /locus_tag="VFQ96_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0257767.1"
                     /translation="MAQLHDLTALELAAALKAGDTDPASAAEHYLARVDRLDGGLGAF
                     VTVTAEAALERARHVRDRVPRTVPMWGVPFADKDLTRRAGVPARFGSRLMAAFVPATS
                     DEIVETLDAAGGVSLGKTSTPEFGLPSYTEPLAGSPARNPWDPSLGAGGSSGGAAVAV
                     AAGLLPVAPGSDGGGSIRIPAAACGLVGIKPSRGRVPAGSGLTSLAGLAVAGPIARTV
                     ADAAWLLDVMMRGAGRQPEHHYGVRAPGPEGPYRAAALADPPRLRIGVMTTSAWDDAY
                     EIVVDPEALKVLGDTARSLDALGHDVAEVALTGSTDYAPAFRTVWQASAAAIPADPEN
                     ELLEPLTRWLIGQGRALGARRLVDALAALKDYERSFIAQFAGYDAILTPALAMTPRPL
                     GWYSDDPEQNFEQQCQYTPYTSMVNVAGLPAVVLPVGQSSEGLPLGVQLIGRPGGEES
                     LVALGGQLERLIGWPRRHPPCW"
     gene            complement(9095..9751)
                     /locus_tag="VFQ96_08015"
     CDS             complement(9095..9751)
                     /locus_tag="VFQ96_08015"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0257768.1"
                     /translation="MDPGWAAALEPVAPRIRAMGDFLRAETAAGRRYLPAGRNVLRAF
                     AAPLDEVRVLIVGQDPYPTPGHPVGLSFAVDAAVRPLPRSLQNIYTELHDDLGIAAPE
                     HGDLTAWSRAGVMLLNRVLTVRAGSPASHRGKGWEAVTEHAIRVLATRGTPLVAVLWG
                     RDAGTLRPLLGGVPVVASAHPSPLSAARGFFGSRPFSRVNRLLAERGAGPIDWRLPPR
                     "
     gene            complement(9843..10622)
                     /locus_tag="VFQ96_08020"
     CDS             complement(9843..10622)
                     /locus_tag="VFQ96_08020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0257769.1"
                     /translation="MTRRAVVTGASSGIGAATARELARRGWQVLGVARRAERLRELAA
                     EGIEYQVVDVTSQGDVDELARRLAPDGVDALVNNAGGARGVDSVEAGSIDDWRWMYEV
                     NVLGFKRVVTALLPLLRARARLAGHADILAVTSTAGHGAYVGGGGYNAAKFAQHAVCD
                     VLRLELNGEPIRVTEVAPGLVATKEFSFNRLGGNRRAADAVYAGVDRPLSAEDVAVTI
                     SDALERPAHVVSDLIVLRPLAQAATTVLHRGPLAVRDDGDV"
     gene            10698..11915
                     /locus_tag="VFQ96_08025"
     CDS             10698..11915
                     /locus_tag="VFQ96_08025"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU0257770.1"
                     /translation="MDWQTSADTAPSSYITEAQARSLLGKPPASGAWRESDPVGGRQF
                     AELGPFTAEAGGSLPHVRVAYESWGTLSPEHDNAVLILHALTGDSHVVGQAGPGHRTP
                     GWWNGIVGPGKAIDTDRWFVAAPNILGGCQGTTGPASLAPDGVEWGARFPFVTIRDQV
                     RAQAMFADALGVTRWAGVVGGSMGGMHALEWACGFPDRVARLAVIAAPADTNADQIAQ
                     NSLQIDAVRTDPGFRGGDYYDADDGDGPHRGLATARRMALLEYRSSHELNARFERGWQ
                     SHLSPLGHGGRFAIESYLDFHGNKFTRRFDANSYITLVEAMNSHDVARDRGSVARALA
                     AARCPSLVVGIDTDRLFPVAEQERVAAGLPGNIDGGHAAVIRSDFGHDAFLIENDLIG
                     GQLRRLFDADAPA"
     gene            complement(11944..12966)
                     /locus_tag="VFQ96_08030"
     CDS             complement(11944..12966)
                     /locus_tag="VFQ96_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEU0257771.1"
                     /translation="MSDVSGRGGADGPRGRDGEAAAARVRLWEAADAARWVAVPGPES
                     DKYSHGVLGVVTGSALFPGAAVLGVEAAHRTGVGMVRYLGDPRPTDLVLRRRPETVMV
                     PGRVEAWLIGSGADRGAVGVLARWREALASGLPVVVDAGALDLVEVGGPGAGSLRPWV
                     LTPHYRELARLLNRLGPAGPADRGEASSRVAAGGAPADGAAPGGFAAGEATPEAIARE
                     PERWARRAARLVRAVVLLKGRATHVATPDGDVSRVVTECGWLATAGTGDVLAGILGAL
                     LATHPAATRPRELAALAATAAFLHGRAGLRASSGGPIAALDVAESLPGAIAELLSSGA
                     LGGRRH"
     gene            complement(13009..14037)
                     /locus_tag="VFQ96_08035"
     CDS             complement(13009..14037)
                     /locus_tag="VFQ96_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0257772.1"
                     /translation="MSGLVWLVVLLAVNAFFVATEFAVVSARRSQIEPLAERGRRSAL
                     VALDAMEHATLMLATTQLGITVCSLLILNLSEPAIHHLLAVPLALTGLPVSAADAVSF
                     VIALVVVSFLHVTFGEMVPKNLSFSMPDRAVLVLATPLRWVARATYPVIWALNEATNG
                     IVRAFGIHPRAEANSTFTLDEVAVIADRSTREGVITDRSGTLGAAFLFGAKRVSDVAV
                     PVDRLVTLPKGVTPQQVERQVARHGFSRYPIVGVSGEPTGYLHLKDVLDLDDTGYRLP
                     VPAKRVRTLASLAAGMELEDALAAMRRSGHHVARVVEGGHVTGVVFLEDVIEELVGEV
                     HDATRRDD"
     gene            complement(14034..>15349)
                     /locus_tag="VFQ96_08040"
     CDS             complement(14034..>15349)
                     /locus_tag="VFQ96_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0257773.1"
                     /translation="LFAAGILLTLGTGVFVASEFSLVNLDRPDIDARHARGERRMGLV
                     AGALHKTSTHLSSAQLGITLTTLLTGFTLEPAIASLLAPAFAFLPPAALAALAALVSV
                     VGATLVSMVIGELVPKNIALALPIPTAKLVLPFQVAFTAVFRPVVKLFDRTANAVLRS
                     VGVTPREELTGARTAAELSSLVRRSAGAGLLEGDTARLLDRTLRLPAHTASDAMTPRP
                     RVSSIGRDDSAADVVTLARTTGFSRFPVLGDDADDVVGVVHVKNAVAVPPAQRGKVPV
                     SALQTAVVRVPETMPLDTLLGELRARGFQMAVVADEYGGTAGIVTLEDLVEELVGEVS
                     DEHDRLRVGVVRRPGSVTFPGLLRPDELAERASLLVPEEGPYETIAGFVVNELGRMPR
                     VGDEVAVAGGTLRVERLDGRRIDRLRFTPDAPPAEGALPEEGAAP"
BASE COUNT         2187 a         5633 c         5455 g         2074 t
ORIGIN      
        1 tttcaccgtg ccgacccgat aaagggtgac accggccggg ccgatgatcc agcgccccgg
       61 ctcgaacgcc atgtcgggca ccggcacccc gagcgcacga cactgcccgg ccacggcccg
      121 cgcgatgccg gccgcgacgt cctcgatggc cgccgggcga tcggccgccg tgtaagcgat
      181 gccgaaaccg ccgcccaggt tcagttgggg cacggggccg cccgcgagca gccgcgcgtg
      241 caggccgacg agacgctcgg cggcccgcgc gaagccttcg atgccgaaga tctgcgagcc
      301 gatgtggccg tgcaggccga ggaacgacag gcccggaagc gaccggatgc gcgccacggc
      361 ggcgggtgcg tcatcgaggc tcaggccgaa cttctggtct tcgtgcgccg tcgccaagaa
      421 ctcgtgggtg tgtgcgtgca cgccgctgtt gacgcgcaac cgcaccgcct ggacccggcc
      481 gtgccgcgcc gccgcctgcg cgaccctctc gatctcgatc ggcgagtcga cgacgatggt
      541 gccgacgccc acgcgaaccg cctcgtcgat ttcggccggc gacttgttgt tgccgtgcag
      601 gccgagccac gcgggctcga cccccgcggc gagggccacg gcgagctcct cgcccgtgca
      661 cacgtcgatg ccgagccccg cctcgcgcat ccagcgggcg ctctgcacgc tcaagaacgc
      721 cttcgccgcg tagtacacgc gcaccgccgc accgagcggt gagaacgcgt cgccgaacgc
      781 ccggaggatg cgccgcgccc gcccgcgcgc ctcatcctcg tcgacgacgt acagcggcgt
      841 gccgtatcgt tcggcgagct ctgtcgcggc gagcccgccg atggtgagcc ggccggccgc
      901 gtcgcgcccg gcacccgacg gccagagtgt gccgggcacg tcgtccccgc ccggcacgag
      961 ccacgcgggc gccagcgggt tccgctcgct cacatccgct ccggtgcgct cacgccgaga
     1021 agctcgaggc cgttgcggat gacctggccg gtggcgtcgt tcagccagag gcgcgtgcga
     1081 tgcacgtcgg tcacgggctc atcgcccaac gggatgacac ggcaggtgtc ataccagcga
     1141 tggtagaggg aggcgatctc ctcgacatag cgggccacgc ggtgcggctc ccgcagccgg
     1201 gccgcttgcg cgacgatcct cggaaactct cgcagcccac tcaaaagcgc cgcctcggtc
     1261 tcctcgccca gcgtctcggg cacgaaggcg ccgcgatcga cgccggccgc cgccgcatgc
     1321 cgatcgacgg aacgcgtgcg ggcatgtgcg tactgcacgt agaagacagg gttgtcgttg
     1381 gctcggcggc gcaactggtc gggatcgagc gtcaacgggg tgtcggccgg cgagcgcgcg
     1441 agcgaatatc gcagcgcgtc ggtgccgagc cacgcgcgca ggtcgtcgag ttcgatgatg
     1501 ttgccggcgc gcttcgacag ccgagcgccg ttcacgctga cgagctggcc gatcagcacc
     1561 tcgatgtcgg cctccgggtc gtctccggcg gcaccggcga gggccttcag ccggccgatg
     1621 tagccgtggt ggtcggcgcc cagcaggtag atcttgtgcg ggaagccgcg ccgtgacttg
     1681 tcgagatagt aggccgcgtc ggcggcgaag tacgtgaaga ctccgttgga gcggcggatg
     1741 acccgatctt tgtcgtcgcc gaagtcggtg gtacgcaccc agaccgcgcc gtctttctcg
     1801 acgacgtgcc cggccgcgcg caggtgctcg acggcacgtt cgatcgcact cttgccttct
     1861 ttgtcggggg tgtgcaagag ccgttcggag aaccagacgt cgaaccgcac gttgaattgg
     1921 gcgagcgact cgcgaatctc ggccagctgg tgtcggtacg cgatctcgcg ggcgagaacg
     1981 acggcctcca ctcgtggcag tgtggcgagg ccgggctcct cctccaacac gacggcggcg
     2041 aggtcgtcga tgtagctgcc cgggtagccg ccctcggggg tcggctcgcc gagggcggcg
     2101 gcgagcaccg actcgccgaa cacgtccatt tggtgcccgg cgtcgttgat gtaatactca
     2161 ctcgtcacct gggcgcccgc ggcacgcagc acgcgggcga tggagtcgcc gagggccgcc
     2221 caccgggtgt ggccgatgtg cagcgggccc gtcgggttcg ccgagacgaa ctccatattg
     2281 acccgcagcc cggcctcgct ctcgccgcgg ccgtagcgct cgccggcccg cacgatggcg
     2341 cgggccagct ccccggcgac gccggcgtcg agacggatgt tgacgaagcc aggccccgcc
     2401 ggccaggcgg aggcgacgcc ggccgcagcc gccaactcgg ccgcgatctc ggctgcgaag
     2461 gcatgcggga cgagcccgaa cgccttcgcg aggcgcaggg cgacgttcgt cgtccagtca
     2521 ccgtgttcgc ggctcttcgg gcgctcgagg acgacgccct ccggcccggg cagccggtcg
     2581 gcggacacgc cgtggcgtgt ggcgacccgg ctgacgatct cgtgaagcgt ggcggggagg
     2641 tcggctgagg tcacgaggat cgattctact aagggagtcg cagcctcgtc gggcgtcgcg
     2701 gttggtagcc tcgccactat gttcagcgcc cgcgacaccc accgtccgct cgccaggcgc
     2761 tcgccggcgc gggccatcgc cgccgcagtc accgtggccg cggcgctcgc catgctcgcc
     2821 gggtgcacgg cacacgccgg gccgtccacg cacactcacg cggccggcac cacgccctcg
     2881 agtcaggccc gccaggtagc ggccgcgccc acgacgcccg ccgccacccc gtcgtccccg
     2941 ccccccacca ccacgacccc gcaggcgacg cccgtcaatg tgtaccgggt ggcctgcagc
     3001 tcgatgatct ccacggcgca gctctacgcc ttcaacccga acgtcagcgc ccagcccggc
     3061 gccacgccgc ccgccggctc tctcgtcgcc caggcggtgg cggccggcgg cacggcctgc
     3121 ggctgggtct atgactcggg aaaccagtcg atccagttcg cgctcgcgac ccccggcccc
     3181 aaggccttgg caagcctcaa agagaaggct gcggccgaca gcaccccgac gagcgcctac
     3241 ggcgacgggc cgaacgtggc cggcttcttc cacgtgacgg gctctcgcgg cgaagtccaa
     3301 gtcttcacga cggggcactg gctcgtcggg ctttcgagca cgttcttcga gccgggagac
     3361 gccgaacaac tggtgcacga catcctcgcc aacctccacc tgggatgacg ggcgagcgga
     3421 cgaatcggcc ccgatgacgc ggctcacggc gacgatcgcc gacgcacgtc gggtgctagt
     3481 cttgtcgcga cccgactgcg ggtcgccggc ccccatagct caggggatag agcgtctgcc
     3541 tccggagcag aaggccgtag gttcaaatcc tactgggggc acgaaggccc aggtggtgcg
     3601 gcacatgcgg ggtgtgcgag gatgaccggc gccgtgcttc tcaacatcgc gttgatcctt
     3661 cttctggccg gatacctggt gcacggctac cgggcgggcc tcacgtggag cctggccggg
     3721 ctcgcgggcc tcgccgcggg ggcggtcgcc tccttcctcg tcgcgccgct tctcgggcag
     3781 agcgcagggg ccagcctctg gcgcgtgccg ctcatcgtcg ccgtcaccgt cgtcttcttg
     3841 gggctcggcc acgccgtggg cagcgcctgc ggccgggtgc tgcgccgcgg tgtgcataag
     3901 acgccgctgc gcctcctcga ccgggcgctg ggtgcagcgg tcgacgtgac cgtcgccgcc
     3961 ctcgtcctcg ccatgctctc gttcggcatc ggctctctcg gcatcccggc gctcagcgcg
     4021 gcggtcggct cttctcccgt gctgcgcacg atcgacgcct tgacgccgga gagtctgcag
     4081 accggcctcg cccggctgaa agacctcgtc acgaaggaca ccgccggacg ggtcttggat
     4141 tctctggcct cgccggctaa gaccccggac ccgacgacca cgacggcgtc tccgacggtg
     4201 ccgaacgtcg acacagacac ggccctgctg cggcaggcgg cgcgttccgt ggtgaagatc
     4261 acaggcctcg ccgccgcgtg ccgacagacc cagtcgggct ccggtttcgt cgcagcgccg
     4321 cggcgggtct tgacgaacgc gcacgtcgtg gcgggcgtca ccgacccggt cgtgcaaggc
     4381 gccgacgggc gctcctacac ggcccgggtc gtcttcttcg acgcccggca cgacgtcgca
     4441 atcctggccg tgccgaggct cacggccccg acgctcgccc tctcgccggt gctggggccg
     4501 gggcaagacg ccgtgttcga cggatacccg ctgggcggtc cgttccgcag cagcccggcg
     4561 gcggtgcgcg gcgtcggcac ggcaaagatc cacgacatct acaacgcgac gaccgtgtcg
     4621 atgccgatct actcgcttgc cgccgacgtc gagcacggca attcgggcgg ccccctcctc
     4681 gcgcccgacg gcagggtcgc gggcgtgatc ttcgccaccg acgcgaacgg ggcggcgatc
     4741 ggctacgcgc tgaccccctc cgtcgtcgac ccggtcgtag aagccgcgcc ccgcgagacg
     4801 acgcccgtcg gcaccggacg atgtgtgccg ggatgaatcg ggcagcccgg ccttcgggcg
     4861 atggcacgaa tttagcggat gatgatactt ttaagcgtca cggcagcgca cagacggcag
     4921 tgcgcggaaa gcggtcgcca tggagaagct ggtcctcgcc tcgacgggcg ccgacgccga
     4981 cgcggtcgtc gcgatcgaac ggcgacacac cgaactcggc acggcggccg cgatgaagac
     5041 cctgcacgtt ctgaacatgg ttccgcggcc ttacgacgtc gtgcgtgcgg ccagggacga
     5101 cctcgtgtgg tgggcctatt cccgcctgca gccgtacttg gagcgcgagg cagatctttt
     5161 tttcccggcg ttgccgagct ccagcgaggc cgacgatgcg gcgcgcgacg cgcggtaccg
     5221 gctcacggag atcgccgcgg ccgtgacgcg cctgcgcacc gacgacaccg cccacgacat
     5281 cgcggccgac gccatcgcgc tacgctccca cttgacggcg ttcttcgaaa cccagcaaca
     5341 ccgcatactg ccgtacctgg ctttttcacg acgacccatc cacgagctct ggcagctcgt
     5401 caacacctga gaagaggatg tccatggagc cgaaccagtt gaccgtgacc cacgacgatg
     5461 gaacgatctg ctcgtgcgcc gagcctgacg atgcgttccc gcccctcgac gtgcgtcagg
     5521 ttccgcattc gatccgccac gcgatcgtcc tcggcgcgct ggatgcggtg aagcccggcc
     5581 tcggcatcgt tctgatcgcg ccacacgacc cgcgccccct cctggcgcag atcgacgccc
     5641 gctaccaggg ccgcttcacc gtggagtacc tggtagagaa gccgaccgaa gtgcacattc
     5701 ggttcacgcg caccgcgtga gcgcgcggct gaccgcgccg ctggccgtga tggtcggcat
     5761 cggcggcgcg gcgatcgtcg ccggcggcat ggtctcggcc gcaaccgggc cgctcggctg
     5821 ggcgaaaggg tcctgggcga gtgcctacct cgtgctcgtc gccggggcgg cgcaggtcgc
     5881 gtacgggctc ttgcagcgcc cgctcgcgag gcgggtgccc tccgcacgga tcgtcgtgtg
     5941 gcagatcgtg ctgttcaacc tcggcaacgc gttggtcttg gcaggctctc tcgcgacgat
     6001 ccccgtggtg gtcgacgtcg gtggcgcggc gatcgtcgcc gcgctggtgc tgttcctggc
     6061 cgcggtgcgc cgccgggccg ccgcgcaccc ggccgccgtg tggacgtatc ggggcctcac
     6121 ggtgcttctc ctcgtgtcga tacccgtcgg cctgaccatc tctaccctgc aggcgtgagc
     6181 gcccgggcgg aggcgaccgg cccggccgca gccgaccttc agcgtcgcag cgacctaggc
     6241 tgacagcatg cgacgtatgg cggcgagcgg cgcggagaat gcttctcccc gcgggccgat
     6301 gccggcatcg gcccgcggaa tctggtccgc cttggcgtac ggcggctacc tctacgcctc
     6361 ggcggtcggg ctggcctggg gcgccctgtg gagcacgggt cgcgtcgaac ggcggggcgg
     6421 tctcgtcgtg ttcacgggcc tgccccgctg ggcgtttcgc cgcgggggat cctgcgtggg
     6481 ctcgtgttac ctcaccttcg acaacgtcag cgacgacgtc ctcgaacacg agcgggtgca
     6541 ccgcggccag tggcgccgct acggcctgct gttccccctc ctgaatctgc tcgcgggatc
     6601 cgatccccat cacaaccgat tcgagatcga ggcgggcctc gagaagggcg gctacctgtg
     6661 agcgacggca caagaaggcg cccgctggtc gtcgtgaccg gtgcgagctc gggcatcggc
     6721 cgtcttgccg ccaggaagct ggcggaggcg ggggcgcggg tcgcgctcgt gggtcgagac
     6781 ccacggcgca cgagcgaagc ggcgctggag gcgggcggca cgccgttcgt cgccgatttc
     6841 gaccggctgg gcgacgtgcg ccggctggcg cagcagcttg acgagctcgg cccgatcgac
     6901 gtcctcgtga acaacgcggg cgggctcgtg cagcaccggg agctcaccgt cgacggcaac
     6961 gaacgcatct tccaggcgaa ccacctggcg ccgttcctcc tgacgaatct gctcctggcc
     7021 gcgcgtcata ggcgaggcga ccaccgaccg ttccgggtcg tctccacggc gagttcggcg
     7081 caccgttacg gcaggctcga ccttcacgat ctgacgttcg cccgaaggcc ctggcgggcg
     7141 ggctgggcgg cctacggagc ggcgaagctc gccaccgtgc ttttcgtgcg tgagcttgcc
     7201 cggcgggccg ctgcggacgg gctggcgggc gaggtcacgg ctttcgccgt gcatcccggc
     7261 ctcgtcgcca ccggcttcgt gcgcgacata ccgtggctgc ggctcgtcgc ccggttgacg
     7321 ggcggccact acgccctcgg cgtcgaggcg ggtgcggcgc cgctcgtccg gctcgcccgg
     7381 cagacccctg gggcgctaag cggcagctac ttcgatcgca tgcgggcggg ccgaggcctt
     7441 gctgcccgcg cccgcggccc cgcggccgac cggctggccc gcgagttgtg gcgggtgagc
     7501 gcccgcctca ccggcctcga cgagcggtag ggcgccggga aggcgaccga tcgggacgcg
     7561 acgccggacg gcgcctcacc agcagggcgg atgccggcgg ggccagccga tcaaccgctc
     7621 gagctggccg cccagcgcga cgaggctctc ctccccgccc ggccggccga tgagttgcac
     7681 gccgagcggc aggccctcgc tgctctggcc gacgggcagc accacggcgg ggagccctgc
     7741 gacgttgacc atcgaggtgt agggcgtgta ctggcactgc tgctcgaagt tctgctcggg
     7801 gtcgtcggaa taccacccca gcggccgcgg tgtcatcgcg agagccggcg tcagaatcgc
     7861 gtcgtagccg gcgaactggg cgatgaacga gcgttcgtag tctttcaacg cggcgagtgc
     7921 gtcgacgagc cggcgcgccc cgagggcccg gccctgcccg atcagccagc gcgtgagggg
     7981 ttcgagaagc tcgttctccg ggtcggcggg gatcgccgcg gcggaggcct gccagacggt
     8041 gcggaaggcg ggcgcgtaat cggtggagcc ggtgagcgcc acctctgcca cgtcgtggcc
     8101 gagcgcgtca aggctgcgtg ccgtgtcgcc cagcaccttg agcgcctccg ggtcgacgac
     8161 gatctcgtac gcgtcgtccc aggcgctggt cgtcatgacc ccgatccgca ggcgcggcgg
     8221 gtcggcgagc gcggctgcgc gatagggccc ttcggggccc ggcgcccgca cgccgtagtg
     8281 gtgctccggc tgccggccgg cgccgcgcat catgacatcc aacagccagg ccgcgtcggc
     8341 gacggtgcga gcgatcggcc ccgccacggc cagcccggcc agcgacgtga gcccgctgcc
     8401 cgccggcacc cgcccgcggg agggcttgat gccgaccagg ccgcaggcgg ccgccgggat
     8461 gcggatcgag cctcccccgt cgctgcccgg cgcgacgggc agcaggccgg ccgcgaccgc
     8521 gacggcggcg ccgccgctgg agccgccggc accgaggctc ggatcccaag ggttgcgtgc
     8581 cggagacccg gccagcggct ccgtgtacga gggcagcccg aactccggag tgctggtctt
     8641 gccgagactc accccgccgg cggcgtcgag agtctcgacg atctcgtcgc tggtcgccgg
     8701 cacgaaggcg gccatcagcc tcgagccgaa gcgtgccggc accccggcgc gacgcgtcaa
     8761 gtccttgtcg gcgaaaggca ctccccacat cggcaccgtg cgcggcaccc ggtctcgtac
     8821 gtgacgggca cgctccaagg ccgcctcggc ggtgaccgtc acgaaggcgc cgaggccgcc
     8881 gtcgaggcgg tcgacacgcg cgaggtaatg ttcggccgcc gaggctgggt cggtatcgcc
     8941 ggctttaagg gctgccgcca actcgagggc ggtcaggtcg tgcagctgcg ccatgccgac
     9001 caccttagac gggtaccccg gccgcgagac gcggggcgag gcggccggcg cgcacccggc
     9061 ccgagtcgcg cggggccggg ccgcccgtcg gcggctatcg gggcggcagc cgccagtcga
     9121 tcggccccgc tccccgttcg gccagaagcc ggttgacacg gctgaacggg cgagagccga
     9181 agaaccctcg ggcggccgag agcgggctgg ggtgggcgga ggcgacgacc ggcacaccac
     9241 ccagaagggg gcgcaaggtg cccgcgtccc ggccccacaa gacggcgacg agcggcgtgc
     9301 cccgggtggc gagaacgcgg atggcgtgct ccgtcactgc ctcccagccc ttgccccggt
     9361 gtgaggcggg tgagcctgcc cgcaccgtga gcaccctgtt gagcagcatc acgccggcac
     9421 ggctccacgc cgtcaagtcg ccatgctcgg gcgccgcgat tccgaggtcg tcgtgcagct
     9481 ccgtgtagat gttttggagg ctgcgcggca ggggacgcac ggccgcgtcg acggcgaacg
     9541 acaaccccac ggggtgcccc ggcgtcgggt aagggtcctg cccgacgatg agcacccgca
     9601 cctcgtcgag gggagccgcg aaggcacgca gcacattgcg gccggcaggc aggtagcgcc
     9661 ggccggccgc cgtctcggca cgcaagaagt cccccatggc acggatacgc ggcgcgacgg
     9721 gctccagcgc cgccgcccag cccgggtcca tggagccgtc ggccgccagc tcggcgaggc
     9781 tcttcggcgc cctgccgacc ccggcctccg ccgagcctcg accgcccgcc gccggcaccg
     9841 actcagacat cgccgtcgtc tcgcacggcg agcgggccgc gatggagcac cgtcgtggcg
     9901 gcctgcgcga gcggccgcaa gacgatcagg tcagagacga cgtgcgcggg gcgttccagc
     9961 gcgtcgctga tcgtcacggc gacgtcctcg gcactcagcg gtcggtcgac gccggcatag
    10021 accgcgtcgg cggcgcgccg gtttccgccg agccggttga acgagaactc cttcgtcgcg
    10081 acgaggccgg gcgccacctc ggtgacccgg atcggctcgc cgttcaattc caggcgcaac
    10141 acgtcgcaga ccgcgtgttg ggcaaacttg gccgcgttgt acccgccgcc acccacgtac
    10201 gcgccgtgcc ctgcggtaga ggtgacggca agaatatccg cgtgcccggc aaggcgcgcc
    10261 cgggcccgca ggagcggcag gagagccgtg acgacccgct tgaagccgag cacgttcacc
    10321 tcgtacatcc accgccagtc gtcgatgctg cccgcctcga cgctgtccac accccgggcg
    10381 ccgccggcat tgttcaccag agcgtcgacc ccgtcgggcg cgagccggcg ggcgagctcg
    10441 tcgacgtcgc cttggctcgt gacgtcgacg acctggtact cgatgccctc cgcagccagc
    10501 tctcggagcc gctcggcccg cctcgcaacc cccagaacct gccagccgcg acgcgccaac
    10561 tccctggccg tcgccgcgcc gattcccgag ctcgccccgg tcaccaccgc ccgtcgtgtc
    10621 atgatcccca ccctagggcg cgtgtcggcg aggcgtaaca atggggcctt cggcgggtag
    10681 ttcggcagaa tgaggggatg gactggcaga cctcggccga cacggcccct tcgagctaca
    10741 tcacggaggc gcaggcccgc tcgctcctcg gcaagccccc ggccagcggc gcgtggcgcg
    10801 aatccgaccc ggtgggcggg cggcagttcg cggagctcgg cccgttcacg gcggaggcgg
    10861 gcggcagcct gccgcacgtg cgcgtcgcct acgagtcgtg gggcacgctc tccccagagc
    10921 acgacaacgc cgtattgatt ctgcacgcgc tgacgggcga cagccacgtc gtcggccagg
    10981 cggggccggg gcaccgcacc ccgggctggt ggaacggcat cgtcgggccg ggcaaggcga
    11041 tcgacaccga ccgctggttc gtcgccgccc cgaacattct gggcggctgc cagggcacga
    11101 ccggcccggc ctcgctcgcg ccggacggcg tcgagtgggg ggcgcgcttc ccgttcgtga
    11161 cgatccgtga ccaggtgcgg gcgcaggcga tgttcgccga tgcgctcggc gtgacgcgct
    11221 gggcgggtgt cgtgggcggc tcgatgggcg gcatgcacgc gttggagtgg gcctgcggct
    11281 ttcccgaccg cgtggcgcgg ctggccgtga tcgccgcccc ggccgacacc aacgccgacc
    11341 agatcgcgca gaactccctg cagatcgacg cggtgcgcac cgacccgggc tttcgcggcg
    11401 gcgactacta tgacgccgac gacggcgacg ggccgcaccg cggcctcgcg accgcccgcc
    11461 ggatggcgct gctcgagtac cgcagttccc acgagctcaa cgctcgtttc gaacgcggct
    11521 ggcaaagcca cctgagcccc ttggggcacg gcgggcgctt cgccatcgag tcgtacctcg
    11581 actttcacgg caacaagttc acgagacggt tcgacgcgaa cagctacatc accctcgtcg
    11641 aggcgatgaa ctcgcacgac gtcgcccgcg accggggctc cgtcgcccgc gcgctcgccg
    11701 ccgcccggtg tccgagcctc gtggtcggca tcgacaccga ccggcttttt ccggtcgccg
    11761 aacaggaacg ggtcgcggcc ggcctgccgg gcaacatcga cggcggccat gccgccgtca
    11821 tccgttcgga tttcgggcac gacgcgttcc tcatcgagaa cgacctgatc ggcgggcagc
    11881 tgcggcgcct cttcgacgcc gacgccccgg cgtaacgcgg cccacgtcgc ggcgatccgc
    11941 cggtcagtgc cggcggcccc cgagggcgcc ggaggaaagc agctcggcga tcgcgccggg
    12001 aaggctttcc gcgacgtcga gcgccgcgat cggcccgccc gacgaggcgc ggagccccgc
    12061 ccgcccgtgc aggaacgcgg cggtggccgc gagagccgcg agctcccggg gccgggtcgc
    12121 cgccggatgc gtggcgagga gcgcgccgag gatgccggcg agcacgtcgc ccgtgcccgc
    12181 agtcgcgagc cagccgcatt ccgtcacgac ccgcgagacg tccccgtcgg gcgtcgcgac
    12241 gtgggtcgcc cgacccttca acaacacgac ggcacgcacg aggcgggcag cacgccgtgc
    12301 ccagcgctcg ggctcccggg cgatcgcctc cggcgtcgcc tcccccgccg cgaacccgcc
    12361 cggcgccgcg ccgtctgccg gcgccccgcc tgccgcgacg cgactcgacg cctcgccgcg
    12421 gtcggcgggg cccgccgggc cgagacggtt cagcaggcga gccagctccc ggtagtgcgg
    12481 tgtcaggacc cagggtcgca gactcccggc gcccgggccg ccgacctcca ccaggtcgag
    12541 cgccccggcg tcgacgacga ccggcaggcc ggaggcgagt gcctcccgcc agcgcgccag
    12601 cacccccacc gcgccccgat cggcgccgct gccgatcagc caggcctcga cacggcccgg
    12661 caccatgacg gtctcgggtc ggcgccgcaa gaccagatcg gtcggccggg gatcaccgag
    12721 gtagcgcacc atgccgacgc ccgtgcggtg agcggcctcc accccgagca cggccgcgcc
    12781 aggaaagagc gccgagcctg tcaccacgcc aagcacgccg tgcgaatatt tgtcgctctc
    12841 cggcccgggc accgcgaccc accgcgcggc atcggccgcc tcccacaggc gcactcgagc
    12901 cgcggccgcc tcgccgtcac gcccgcgcgg cccgtccgct cccccgcgcc cactcacgtc
    12961 gctcatgctg agcctccgtc tcgtcgtgcc gaccacacct gccggcgctc agtcgtctcg
    13021 tcgcgtcgcg tcatgcacct cgccgacgag ttcctcgatc acgtcttcca aaaagacgac
    13081 gcccgtcacg tgtccgccct cgacgacgcg ggccacgtgg tggccgctgc gacgcatcgc
    13141 cgccagcgcg tcttccaact ccatgcccgc cgcgagcgag gcgagcgtgc gcacccgctt
    13201 cgccggcacc ggcagccggt aaccggtgtc gtcgaggtcg agcacgtcct tcaggtgcag
    13261 gtagcccgtg ggctcgccgc taacgccgac gatcggatag cgcgagaagc cgtgccgggc
    13321 gacctgacgc tccacctgtt gaggagtcac gcctttcggc agggtgacga gccgatcgac
    13381 cggcacggcg acgtcactca cccgcttcgc cccgaagaga aacgcggcgc cgagggtgcc
    13441 ggaccggtcc gtgatgacgc cttcgcgggt ggagcggtcg gcgatcacgg ccacttcgtc
    13501 gagcgtgaac gtactgttcg cctctgccct cgggtggatg ccgaacgccc ggacgatgcc
    13561 gttcgtggcc tcgttcaagg cccagatgac cgggtaggtg gcgcgcgcga cccaccgcag
    13621 cggggtcgcg agcacgagca ccgcacggtc gggcatcgag aacgacaggt tcttcggcac
    13681 catctcgccg aaggtcacgt gcaagaacga gacgacgacg agggcgatga cgaacgagac
    13741 ggcgtcggcg gcggacacgg gcaggccggt caaggcgagc ggcacggcga gcagatggtg
    13801 gatcgccggc tcggagaggt tcaagatcag cagcgagcac acggtgatgc ccagctgggt
    13861 ggtggcgagc atcagcgtcg cgtgttccat ggcgtcgagg gcgacgagcg cgctgcgccg
    13921 gccgcgttct gcaagcggct cgatctgcga cctgcgggcg gagaccaccg cgaactcggt
    13981 ggcgacgaag aacgcgttga cggccaggag caccacaagc cacacgaggc cgctcatggt
    14041 gccgccccct cttcgggcag cgcgccctcc gcaggcgggg cgtcgggggt gaagcgcaga
    14101 cggtcgatgc gccgcccgtc gagccgctcg acgcgcagcg tgccgcccgc gaccgcgact
    14161 tcgtcgccca cccgcggcat ccgccccagc tcgttcacga cgaagccggc gatcgtctcg
    14221 tacggcccct cttccggcac gaggagcgac gcgcgttccg cgagctcgtc aggccgaagc
    14281 aggccgggga acgtgacgga gccgggccgg cgcaccacgc cgacccgcag ccggtcgtgc
    14341 tcgtcggaga cctcgccgac gagctcctcg acgaggtctt cgagagtcac gatgccggcg
    14401 gtgccgccgt actcgtcggc gacgaccgcc atctggaatc cgcgggcccg caactccccg
    14461 aggagggtgt cgagcggcat cgtctcggga acccgcacga cggcggtctg cagcgcggag
    14521 acgggcacct tgccgcgctg tgccggcggc accgcgaccg cgttcttcac gtggacgaca
    14581 cccacgacgt cgtcggcatc gtcgcccagc acgggaaagc gggagaagcc cgtggtgcgg
    14641 gcgagagtca cgacgtcggc cgcgctgtcg tcacgcccga tgctcgagac acgagggcgc
    14701 ggcgtcatcg cgtcggaggc ggtgtgtgcc ggcagccgca gcgtgcgatc gagaagccgt
    14761 gccgtgtccc cctcgaggag gccggcgccg gccgagcggc gcacgaggga cgagagctcg
    14821 gcggccgtgc gcgccccggt gagctcctcc cggggcgtca ccccgaccga gcgcagcacg
    14881 gcgttcgcgg tgcggtcgaa gagcttcacg accgggcgga agaccgccgt gaacgccact
    14941 tggaacggca gcaccagttt cgccgtcggg atcggcaagg ccaacgcgat gttcttcggc
    15001 accagctccc cgatcaccat cgagacgagc gtggcgccga ccaccgagac gagtgccgcg
    15061 agggccgcta gcgccgcggg cggcaggaag gcgaacgccg gcgccagaag cgaagcgatc
    15121 gccggctcca acgtgaaccc ggtcaggagg gtcgtgagag tgatgcccag ctgtgcgctc
    15181 gacagatggg tggaggtctt gtgcagggcc ccggcaacaa ggcccatgcg acgctcgccg
    15241 cgagcgtggc gggcgtcgat gtctgggcgg tcgaggttca cgagagagaa ctcgctggcg
    15301 acgaacacac cggtgccgag cgtcaacagg atccctgcgg cgaaaagca
//
LOCUS       DASTNL010000246         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_923990, whole genome shotgun sequence.
ACCESSION   DASTNL010000246 DASTNL010000000
VERSION     DASTNL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923990"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFQ96_08045"
     CDS             complement(<1..1323)
                     /locus_tag="VFQ96_08045"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU0257774.1"
                     /translation="MAGGGTTRDRETRGHEAAGRQAFGASRHSFRPEVQALRALAVAL
                     VVVFHLWPGALPGGFVGVDVFFVISGFLITGQLLREAGRLGRIRLGGFWARRVRRLLP
                     AALASLAATGIATWLVAPQTVWRQYFSDIAASAAYVQNWHLVAQAVDYFAPSGASPVQ
                     NFWSLAVEEQFYLVWPLLLVVALVATRRRPGARRRAFVAVLGAASVASLIYCVVVSRD
                     AAQIAYLSTLARGWEFGLGGLLALVLPRLSRLGERAASRNGWAAALSGARAALSWCGV
                     AVIVVSAFLLNSGTAFPGAVALWPTLATCVVIAAGVPRGRWAPGRAMTLPAVQWLGGA
                     SYSLYLWHWPVIVLAPFAWERIAGRSAPAWSSLLVLALSLALAWASKRWIEDRTRTAA
                     FWSGPKPTRSFAVMAAGMMVVGGLALACAGTVQTTIDVSLDRAANVVRV"
     gene            1598..1942
                     /locus_tag="VFQ96_08050"
     CDS             1598..1942
                     /locus_tag="VFQ96_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0257775.1"
                     /translation="MSEDAIVSEPASSAPVVREPRIGTPPAPDHFRLGATSTLRHRGV
                     QYRTELVADDTWEVRGEDGRVLGSVVIVSPAGEEGEAVYGSRSGSSDTVEYEGTDWRS
                     VTAGMINQVLDA"
     gene            complement(1993..3192)
                     /locus_tag="VFQ96_08055"
     CDS             complement(1993..3192)
                     /locus_tag="VFQ96_08055"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU0257776.1"
                     /translation="MTANVSEAAIGIHDLALATGHHVLDLRQLAEHNGSEVDKYYIGI
                     GQSQMSVAAEDEDVVTLGASAARRLLDRRADGAGHPVRTLLFATESGIDQSKSAGVYV
                     HRLLGLDPACRVVELKQACYSATAALQFALGLVARDRRESVLVIAADVARYPLDSPGE
                     ATQGAAAAAFLVGADPALVAIEPATGLHTDDVHDFWRPNDRSEAIVDGKRSLGSYLRS
                     LEGSWLDYRGRGGVDFEAIDRFCYHQPFTKMARKAHDRLCRIVGAALGPEDRARQLGA
                     SLELNRRLGNSYTASLYLGLLSLLEGERGLGGSRVGFFSYGSGSVSEFFTGVVRPGYE
                     SVVRAAEDEQILARRVPVSYERYSALHRKVDEVAGTDYATAAETTGPFRFAGVEGSVR
                     RYERRAA"
BASE COUNT          524 a         1150 c         1201 g          404 t
ORIGIN      
        1 gacgcgcacg acattcgccg cgcggtcgag gctcacgtcg atcgtcgtct gcaccgttcc
       61 ggcgcaggcg agcgcgaggc cgccgacgac catcatgccg gccgccatca cggcgaaact
      121 gcgggtgggc ttcggccccg accagaaggc cgccgtgcgg gtgcggtctt cgatccaccg
      181 tttgctcgcc caggcgagcg cgagagagag cgcgagcacg agcaggctcg accacgcggg
      241 ggctgaccgg cccgcgatgc gttcccaggc gaacggggcg agcacgatga ccggccagtg
      301 ccacaggtag agcgaatacg acgcgccgcc cagccattgc acggcgggca aggtcatcgc
      361 ccggccgggt gcccaccggc cgcgcggcac gcccgccgcg atgacgacac aggtggccag
      421 cgtcggccag agggcgacgg cgccgggaaa ggccgtgccg gagttcagaa ggaatgcgct
      481 gacgacgatg accgccaccc cgcaccacga cagcgccgcc cgcgccccgg agagcgcggc
      541 cgcccacccg ttgcgggagg cggcgcgttc cccgagcctc gacaagcgcg gcaggacgag
      601 ggcgagcagg ccccccagcc cgaactccca cccgcgggcc agcgtggaaa ggtaggcgat
      661 ctgtgccgcg tcccggctga cgacgacgca atatatgagg ctcgcgacgc tcgccgcgcc
      721 cagcacggcg acgaacgccc gccggcgggc tccgggccgt cgccgcgtcg cgacgagcgc
      781 gacgacgaga aggagcggcc agacgaggta gaactgctcc tcgacggcga gcgaccagaa
      841 gttctgcacg ggcgaggcgc cggagggggc gaagtagtcg acggcctggg cgacgagatg
      901 ccagttctgc acgtaggcgg cgctcgccgc gatatcggag aagtactgcc gccacacggt
      961 ctgcggagcg acgagccacg tggcgatccc ggtcgccgcg agcgaggcga gcgcggccgg
     1021 caggagacgc cgcacccgcc tcgcccagaa gccgcccagc cggatgcggc cgaggcggcc
     1081 ggcctcgcgc aacagctggc cggtgatgag gaagccagag atgacgaaga agacgtcgac
     1141 accgacgaag ccgcccggca gggcgcccgg ccacaggtgg aagacgacga cgagggcgac
     1201 ggccagggcg cgcagcgcct gcacctcggg gcggaacgaa tgtcgcgatg cgccgaaggc
     1261 ctgcctgccg gcagcctcgt gccctcgtgt ctcccggtcg cgcgtcgtgc cgcccccggc
     1321 catccggccc cttcccgccc gccgtgacgc cgaggccgcg acaggctgat ccttgtcgac
     1381 gatcgcttcg ttggcctcgg ccgtcagcgt aacaagcaca cgtttgagct cccttaggag
     1441 gtggtggcag gccgccgaca ccgtgccacg tcgccgcata cgtgaatctt tctttaggac
     1501 ggcccggtaa gcgcccagac catggcatca gccggcgggc cgctcataga ctgaagccgg
     1561 ggttgccgct caagggcggc gggaaggagt cggttcgatg tctgaagacg cgatcgtgtc
     1621 ggagccggcc agttcggcac ccgtcgtccg ggagccgcgg atcggcacgc cgccggcgcc
     1681 cgatcacttc cgtctcggcg cgacgagcac gctgcggcat cgcggcgtgc aataccgcac
     1741 cgagctggtg gccgacgaca cctgggaggt gcggggcgaa gacggccggg tactcggctc
     1801 tgtcgtgatc gtctcccccg cgggcgaaga aggcgaggcg gtgtacggct cccgcagcgg
     1861 gtcctccgac acggtggagt acgagggcac cgattggcgc agcgtgaccg ccggcatgat
     1921 caaccaggtt ctcgacgcct gaacccgccg ccgtgccggg gcccggcggc aagccagggg
     1981 cacaccgaac ggtcaggcgg cacggcgctc gtagcgcctg acgctgcctt cgacgcccgc
     2041 gaagcggaac ggccccgtcg tctcggcggc ggtcgcgtag tcggtgcccg ccacctcgtc
     2101 caccttgcgg tgcagggcac tgtaccgctc gtaggagacc ggcacgcgcc gggcgaggat
     2161 ctgctcgtct tcggccgcac gcacgacgct ctcgtagccg ggtcgcacga ccccggtgaa
     2221 gaactcggag acgctgccgg agccgtagct gaagaagccg acccgactcc ccccgagccc
     2281 ccgctccccc tcgagcagcg agaggaggcc gaggtagagg gaggcggtgt agctgttgcc
     2341 gagccggcgg ttcagttcga ggctcgcacc cagctgccgg gcgcgatcct cggggccgag
     2401 ggctgccccg acgatgcggc agagccggtc gtgcgccttc cgcgccatct tcgtgaatgg
     2461 ctggtggtag cagaaccggt cgatcgcctc gaagtcgacg ccgccgcggc cccggtagtc
     2521 gagccacgac ccctccaacg agcgcaggta ggagccgagc gagcgtttcc cgtcgacgat
     2581 cgcctcgctg cggtcgttcg gccgccagaa gtcgtgcacg tcgtcggtgt gcaggccggt
     2641 ggccggctcg atggcgacga gcgccgggtc ggcgccgacg aggaatgcgg cggccgcggc
     2701 gccttgcgtc gcctcgccgg gcgagtcgag cgggtaacgg gcgacgtcgg ccgcgatgac
     2761 gagcacgctc tcccgccggt cgcgggcgac gaggccgagc gcgaactgca gggcggccgt
     2821 ggccgaatag caggcctgct tcagctcgac gacgcggcag gccgggtcga ggccgagcag
     2881 ccggtgcaca tagaccccgg ccgatttgga ttggtcgata cccgattcgg tggcgaaaag
     2941 gagcgttcgc acggggtggc cggccccatc ggcacggcgg tcgaggagac ggcgggcggc
     3001 gctcgccccg agggtcacga catcctcgtc ctcggcggcg acgctcatct gtgactggcc
     3061 gatgccgatg tagtacttgt cgacctcgct gccgttgtgc tcggcaagct ggcgcaggtc
     3121 gagcacgtga tgacccgtag cgagggccag atcgtggatg ccgatcgccg cctccgacac
     3181 gttcgccgtc accgaccgct cctctcaagg tgaagatggg cggccatcag ctcgcccggg
     3241 ttcgtctgcg cggccatcag cgagagctcc ccgcagagc
//
LOCUS       DASTNL010000247         7363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_924815, whole genome shotgun sequence.
ACCESSION   DASTNL010000247 DASTNL010000000
VERSION     DASTNL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924815"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..691)
                     /gene="lexA"
                     /locus_tag="VFQ96_08060"
     CDS             complement(29..691)
                     /gene="lexA"
                     /locus_tag="VFQ96_08060"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU0257777.1"
                     /translation="MAAKKTARPDGRRPLSAKQVEIVSVIRRSIERRGYPPSMREIGD
                     AVGLASLSSVTHQLNRLEAAGCLKRDANKPRALVLTPAAAKQGNPADEAPADDVALVP
                     LVGRIAAGVPITAEQEIEEVFPVPRRLVGGGELFMLRVVGESMIDAAICDGDWVVVRQ
                     QNDAENGDIVAALLGDEATVKVFRQKDGHTWLLPRNSTFDPIPGDRARVLGRVVAVMR
                     QL"
     gene            977..1357
                     /locus_tag="VFQ96_08065"
     CDS             977..1357
                     /locus_tag="VFQ96_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU0257778.1"
                     /translation="MDSMLARGAGVGAETRTSEARLRLTRRGRAVLTTLVAAPAVVLV
                     VAASLLAGAAQAAGRDSAAAAAAYSHSVTVQQGDSLWSVAMRVAPDDDPRIVVRDIAQ
                     LNDVTGTALTPGERLAIPERYTGR"
     gene            1374..2471
                     /locus_tag="VFQ96_08070"
     CDS             1374..2471
                     /locus_tag="VFQ96_08070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0257779.1"
                     /translation="MTTLADLPIRDDLRGRSPYGAPQIRVPVALNVNENTHPLPEAVV
                     DEITAALRVAVRGANRYPDREFDGLREALAGYLGDGVEPGQVWAANGSNEILQQVLQA
                     FGGHGRTLMGFAPTYSMYPLLASGTGTAWLAGHRDEGFELSARTAAEQVAQARPDIVV
                     LCAPNNPTGTPLGLDVVEAVYGAFDGIVVVDEAYWEFMPRGTASATSLLPGRPRLVVS
                     RTMSKAFAFAGVRLGYLACDPAVADALRLVRLPYHLSALTQAAATVALRHAGEMLAVV
                     DDIVAQRDRISRELTRLGFHPYRTGSNFVFFGGVPHPRQVFESLLADGVLVREQSIPG
                     HLRVSAGTAEETGAFLDAIARLAPLAAHPVS"
     gene            2482..3090
                     /gene="hisB"
                     /locus_tag="VFQ96_08075"
     CDS             2482..3090
                     /gene="hisB"
                     /locus_tag="VFQ96_08075"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEU0257780.1"
                     /translation="MTSSGRIASIHRATNESDIELTLALDGTGTAQIDTPVPFFNHML
                     NAFTKHALVDLTVRATGDTEIDVHHTVEDTGIVLGHAIREALGDKRGIRRFGDALVPL
                     DEALAQAVVDISGRPFLVHSGEPAGFEYHLIGGHFTGSLVRHVFEAIVFGAALTSHIT
                     VLAGRDPHHIAEAEFKAFARAFRQAKEHDPEVTGVPSTKGVL"
     gene            3087..3722
                     /gene="hisH"
                     /locus_tag="VFQ96_08080"
     CDS             3087..3722
                     /gene="hisH"
                     /locus_tag="VFQ96_08080"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU0257781.1"
                     /translation="MSAPSVVVFDYGSGNVHSARKALEAAGARVELTGDRQRALDADG
                     LVVPGVGAFGAVVRALRQKHGDEIIGRRLAGGRAVLGICVGMQVLFDVGLEGAEPTAG
                     MGEWPGRVEALHSAVLPHMGWNTVDAPAESVLFRGIGDRRFYFVHSYAARAWQMPADD
                     RLTPAMLTWATYGEPFLAAVENGPLSATQFHPEKSGDAGLQLLRNWLGTLA"
     gene            3763..4506
                     /gene="priA"
                     /locus_tag="VFQ96_08085"
     CDS             3763..4506
                     /gene="priA"
                     /locus_tag="VFQ96_08085"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEU0257782.1"
                     /translation="MSEFNNTPGLTLLPAVDVAGGKAVRLTRGELGSETSFGDPVEAA
                     ANWVAEGAEWIHLVDLDAAFGRGDNHALLKRVIRQVKGVNVELSGGIRDDESLEAALG
                     IGAKRINLGTAALENPEWAANVIGQYGEAVAVGLDVRGSTLAARGWTREGGDLWQVIE
                     RLEAAGCARYVVTDVTKDGTLQGPNLDLLRRVMERTARPVVASGGIASLDDIAALREL
                     VPAGLEGAIVGKALYAGSFTLREALDVAG"
     gene            4493..5533
                     /locus_tag="VFQ96_08090"
     CDS             4493..5533
                     /locus_tag="VFQ96_08090"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEU0257783.1"
                     /translation="MSPAEPTGEPSDGVPHDAAVPTGGRIEGQTPEERDFGSGRGHAD
                     GHEYVFETGADRPARRPGGSRLTGTPHRLGREHVAKEGKPTRRTLPAHFHYLGMTDSA
                     GRPWQGRHFTRDRAYAEDDGQAPEALLTAVEDFRVGLADASVVIEAIRASRLLIPLMA
                     QLRRAGQNELGMAVDKANELAIVLVRAPDGRTVLPAFSSAAAMRSWDADARAMPAAGP
                     RIALAAASEHADVLILDPGRPAEFAVRRPALWALAQGAPWLPPLRDPEVARAFHASVD
                     GDSVVRDLALVDGDPEARLHGPEVIVRLRLTPGLTKEQLDAGVRALSERWAADEVIAQ
                     RVDSLAVRLDAA"
     gene            complement(5547..5894)
                     /locus_tag="VFQ96_08095"
     CDS             complement(5547..5894)
                     /locus_tag="VFQ96_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEU0257784.1"
                     /translation="MTDPDDSVRQATRDIADVPAAEVITTTAVHLMSAAAVKCGLAEE
                     GPATQDLAEARALIEALAGLVMASEHYVGNMHFWSLRDGLRTLQLAFREASAIPDAPG
                     AGPGERLTGPVRA"
     gene            6150..6791
                     /gene="infC"
                     /locus_tag="VFQ96_08100"
     CDS             6150..6791
                     /gene="infC"
                     /locus_tag="VFQ96_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU0257785.1"
                     /translation="MSEPRTNDRIRVPEVRLVGPGGEQVGVVKIGVALRLAQDAELDL
                     VEVAPNSKPPVVKIMDYGKYKYEAAQKAKEARRNQANTVLKEVRFRLKIDTHDYETKR
                     KRAEGFLRSGDKVKAMILFRGREQSRPELGVRLLQRFAEDVGEYGSVESTPKIDGRNM
                     VMIVGPVKNKAEARAEANAQRGATRERQVPRRNRPARADGQQGSPAQATNEEK"
     gene            6791..6985
                     /gene="rpmI"
                     /locus_tag="VFQ96_08105"
     CDS             6791..6985
                     /gene="rpmI"
                     /locus_tag="VFQ96_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0257786.1"
                     /translation="MPKQKTNSGAKKRFRLTGSGKVMKQQSGMRHNLEVKASRRKRRL
                     NQDQLVSPADAKNVKRLLGK"
     gene            7042..>7363
                     /gene="rplT"
                     /locus_tag="VFQ96_08110"
     CDS             7042..>7363
                     /gene="rplT"
                     /locus_tag="VFQ96_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU0257787.1"
                     /translation="MARVKRAVGAHKKRRVILERAEGYRGLRSRLYRKAKEQVTHSLT
                     YSYRDRRARKGDFRRLWIQRINAASRQNGLTYNRFIQGLGLAGVEVDRKILADLAVND
                     APAFA"
BASE COUNT         1171 a         2541 c         2553 g         1098 t
ORIGIN      
        1 gcggagttgg ccggaactgg ggccgtggct acagctggcg catgaccgcg accacccggc
       61 ccagaacccg ggcgcggtcg cccgggatcg ggtcgaaggt gctgttgcgc ggcagcagcc
      121 atgtgtgccc gtctttctgc cggaacacct tcacggtcgc ctcgtcgccc aagagcgccg
      181 ccacgatgtc gccgttctcg gcgtcgttct gctgccgcac gacgacccag tcgccgtcgc
      241 agatcgctgc gtcgatcatc gattcgccta ccacgcgcag catgaagagc tccccgccgc
      301 ccacgaggcg gcgcggcacg ggaaagacct cttcgatctc ctgctccgcc gtgatcggca
      361 ccccggcggc gatgcggccg acgagtggca cgagggcgac gtcgtcggcc ggcgcctcgt
      421 cggccggatt gccctgcttg gccgccgccg gggtcagcac gagcgctcgg ggcttgttcg
      481 cgtcgcgctt caggcagccg gccgcctcga gtctgttgag ctggtgggtc acgctcgaga
      541 gcgaggcgag gcccacggcg tcgccgatct cccgcatgct cggcgggtag ccccggcgtt
      601 cgatcgagcg tcgtatcacc gaaacgatct cgacctgctt ggcactcagg gggcgtcggc
      661 cgtcgggacg cgccgtcttc ttcgccgcca tctcgcccga cccttctctg acggatctct
      721 catcggcccg ccggatcccg cagcccggcc gcgtggctcg caccggaatg tcggtggtcc
      781 ctgatgaact ccccgagtca tcgaaacact atcgaagtag ctcctcgaag gcaaacatgt
      841 gttcgaaaga atgtcttgcc agagaggaat atcgaagata tattcgaaac aagcgttcga
      901 ctcgagctcc gggctcggcg gacgaaggct tggtgagaga gaccgaccgc gtggtgaggc
      961 agagaagagg cggtggatgg actcgatgct ggcccgcggc gcgggtgtgg gcgccgagac
     1021 gcgcacgagc gaagcgcggc tgcggctgac acgccgcggc agggccgtct tgacgacgct
     1081 cgtggccgcg cccgcggtcg tgctcgtcgt cgcggcctct cttctggccg gtgcggcgca
     1141 ggcggccggc cgagactccg ctgcggccgc cgcggcctat tctcacagcg ttacggtgca
     1201 gcagggcgat tccttgtggt cggtggcgat gcgcgtggcg cctgacgatg acccgcggat
     1261 cgtcgtgcgc gacatcgccc agctgaacga cgtgaccggc acggccctga cgccggggga
     1321 gcgcctcgcg atacccgaga ggtacacggg gcgctgacgc ctagcatgaa ggggtgacga
     1381 cactggccga cctgccgatc cgcgacgacc tccgcggtcg ttccccctac ggcgcaccgc
     1441 aaatacgggt gcccgtcgct ctgaatgtga acgagaacac gcatccgctg cccgaggcgg
     1501 tcgtcgacga gatcacggcc gcgttacgcg tcgccgtgcg cggcgcgaac cgctacccgg
     1561 atcgcgagtt cgacggcctg cgcgaggcgc tggccggata cctgggtgac ggggtcgaac
     1621 cggggcaggt gtgggcggcc aacgggtcga acgagatcct ccagcaggtc ttgcaggctt
     1681 tcggcggcca cgggcgcacc ctcatgggtt tcgcgccgac ctattcgatg taccccttgc
     1741 tggcctccgg caccggtacg gcgtggctcg caggacaccg cgacgagggc ttcgagttga
     1801 gcgcccggac ggccgcggag caggtcgcgc aggcacgccc cgacatcgtc gtgctctgtg
     1861 cgccgaacaa tccgaccggc acgcctctcg gcctcgacgt ggtcgaagcc gtctacggcg
     1921 ccttcgacgg catcgtcgtc gtcgacgagg cgtattggga gttcatgccg cgcggcacgg
     1981 cctccgccac gagcctcctg cccggccggc cccgcctcgt cgtctcccgc acgatgagca
     2041 aggccttcgc cttcgccggc gtgcgtctcg gctatctggc ctgcgacccg gccgtcgccg
     2101 atgccttgcg cctcgtgcgc ctgccgtacc acctctccgc cctgacgcag gccgccgcga
     2161 ccgtcgcgct ccggcacgcc ggcgagatgc tcgcggtcgt cgacgacatc gtcgcccagc
     2221 gagaccggat cagccgggag ctcacaaggc tcggctttca cccgtaccgc acgggcagca
     2281 acttcgtctt cttcggcggc gtgccacacc cgaggcaggt cttcgagtca cttctcgccg
     2341 acggcgtgct ggtgcgggag cagagcatcc ccgggcacct tcgggtcagc gccggcacgg
     2401 ccgaagagac gggcgcgttc ctcgatgcca tcgcccgcct tgccccgctg gcggcccacc
     2461 cggtgtccta aactcgatcc catgacctcc tccggccgca tcgcgtcgat tcaccgggcg
     2521 acgaacgagt ccgacatcga gctcacgctg gcgctggatg gcacgggcac tgcgcagatc
     2581 gacactcccg tgccgttctt caaccacatg ctgaacgcgt tcacgaagca cgccttggta
     2641 gacctcacgg tccgcgcgac gggcgacacg gagatcgacg tgcaccacac agtcgaagac
     2701 accggcatcg tcctcggaca cgcgattcgc gaggcgctcg gcgacaagcg cggcatccga
     2761 cgcttcggcg acgcgctcgt cccgctcgac gaggcgctcg cgcaagcggt cgtcgacatc
     2821 tcgggccgtc ccttcctcgt ccattccggg gagccggcag gcttcgaata tcacctcatc
     2881 ggcggccact tcacgggctc ccttgtgcgc cacgtgttcg aggcgatcgt cttcggtgcg
     2941 gcgctcacca gccacatcac ggtgttggcg gggcgcgacc cccaccacat cgccgaggcg
     3001 gagttcaagg cgttcgcgcg cgccttccgg caggcgaaag aacacgaccc cgaggtgacg
     3061 ggcgtgccct caacgaaagg cgtcttgtga gcgcaccctc cgtggtcgtc ttcgactacg
     3121 gctccggcaa cgtccactcg gcgcggaagg cgttggaggc cgcgggagcc agggtcgagc
     3181 tgaccggcga tcggcagcgc gcgctcgacg ccgacggtct cgttgtgccg ggggtcggcg
     3241 cgttcggcgc ggtcgttcgc gccctgcggc agaagcacgg cgacgagatc atcggccggc
     3301 gcctcgccgg gggccgtgcc gtgctgggca tctgcgtcgg catgcaggtg cttttcgacg
     3361 tggggttgga aggcgccgaa cccaccgcgg gcatgggcga atggccgggc cgggtcgagg
     3421 cgttgcattc tgccgtcttg ccgcacatgg ggtggaacac ggtcgacgcc ccggccgaat
     3481 ccgtgctttt tcgcggcatc ggcgaccggc gcttttactt cgtccactcc tacgcggccc
     3541 gcgcctggca gatgcccgcc gacgaccgac tgaccccggc gatgctgacc tgggcgacct
     3601 acggcgagcc cttcctcgcg gccgtcgaga acggtccgct gagcgcgacg cagtttcacc
     3661 ccgagaagtc cggcgacgcc ggccttcagc tcctgcgcaa ttggctgggc acgctcgcct
     3721 gaggcgagcg gacctaccgt aatgcgccct atgaggaaga cgatgagcga gttcaacaac
     3781 accccgggcc tgacactctt gcccgcggtc gacgtggccg gcggcaaggc cgtgcgcttg
     3841 acccgtggag agcttggcag cgagacatcc ttcggcgacc cggtcgaagc ggccgcgaac
     3901 tgggtcgccg agggcgccga atggatccac ctggtcgacc tcgacgcggc gttcggcaga
     3961 ggcgacaatc acgccctgct gaagcgcgtc atcagacagg tcaagggggt gaacgtcgag
     4021 ctgtcgggcg gcattcgcga cgacgagagt cttgaggcgg ctctcggcat cggcgccaaa
     4081 cgcatcaacc tcggtacggc ggcgctggaa aaccccgagt gggcggccaa tgtgatcggt
     4141 caatacgggg aggccgtcgc cgtcgggctg gacgtgcgcg gcagcacgct ggcggcgcgt
     4201 ggctggaccc gagagggcgg cgatctctgg caggtcatcg agcggttgga ggccgccggc
     4261 tgcgcccgtt acgtcgtcac cgacgtcacg aaagacggca cgttgcaagg gcccaacctc
     4321 gaccttctgc gtcgcgtgat ggagcgcacc gcgcgcccgg tcgtcgcctc cggcggtatc
     4381 gcgagcctcg atgacatcgc ggcgctgcgg gagctggtgc cggcaggcct cgagggggcg
     4441 atcgtcggca aggccctcta cgcggggtcg ttcacgctgc gcgaggctct cgatgtcgcc
     4501 ggctgagcct acgggggagc cctccgacgg ggtgccgcac gacgcggcgg tgcccacggg
     4561 cggccgcatc gaggggcaga cgcccgaaga acgtgatttc ggcagcggcc gcgggcatgc
     4621 cgacgggcac gagtatgtct tcgagacagg tgccgacagg ccggccaggc ggccgggcgg
     4681 cagcaggctc acggggacgc cgcaccggct gggccgggag catgtggcga aagagggcaa
     4741 accgacgagg cgcaccctgc cggcgcattt ccactacctg ggaatgaccg actcggccgg
     4801 caggccgtgg cagggccggc atttcacccg cgaccgcgct tacgccgagg acgacgggca
     4861 ggcgcccgag gccctcctga cggccgtgga ggactttcgc gtcgggctcg ccgacgcctc
     4921 cgtggtgatc gaggcgatcc gcgccagccg gctgctgatt cccctcatgg cgcaactgcg
     4981 ccgggccggg cagaacgagc tcggcatggc cgtcgacaag gccaacgagc tggcgatcgt
     5041 cctcgtgcgg gcgcccgacg gccgcaccgt cctacccgcg ttcagctccg ccgccgccat
     5101 gcgctcatgg gatgccgacg cgcgggcgat gccggccgca ggcccccgca tcgcgctcgc
     5161 ggccgccagc gaacacgccg acgtgttgat cctcgacccg ggcaggcccg cggagttcgc
     5221 cgtacgacgg cctgccttat gggccctcgc ccagggcgca ccctggctgc cgccgttgcg
     5281 cgaccccgag gtggcgcggg ccttccacgc ctcggtcgac ggcgacagtg tggtgcggga
     5341 tcttgccctc gtcgacggcg acccggaggc tcgattgcac gggcccgagg tcatcgtgcg
     5401 gctgcgcctt actccggggc tcacgaaaga gcagctcgat gccggcgtgc gggccctctc
     5461 cgagcgttgg gcggccgacg aggtaatcgc gcagcgcgtc gattccctgg ccgtgcggct
     5521 cgacgccgcc tgacacggcg ggcgcttcag gcgcggaccg ggcccgtgag cctctcgccg
     5581 gggccggcac ccggggcgtc ggggatcgcc gaggcctctc ggaacgccag ctgcagcgtg
     5641 cgcaacccgt ctcgcaggct ccagaaatgc atgttgccga cgtagtgctc gctggccatg
     5701 acgagcccgg cgagcgcttc gatgagcgcc cgcgcctcgg cgaggtcctg ggtggccggc
     5761 ccctcctcgg caaggccgca tttcaccgcg gccgcactca tgaggtgcac cgcggtcgtg
     5821 gtgatcacct cggcggccgg gacgtcggcg atgtctcgtg tcgcctggcg cacggagtcg
     5881 tcgggatccg tcatcgtcgc ggcgtgctct tccggtgctg ggctcggcgt atcgctcact
     5941 ggcttcctct gctacactcg gtgcggatcc tgagaccctg tctcagggat gcaaagtgga
     6001 gatcctccca cccgcgcttg accgtttatc aggttaccgg gttcattgca ctccgcccgg
     6061 cgagatgcgg gtagaaaggg tgcggccgtc acagcggcct gggacctctg cctggcatcg
     6121 tacgcaagag cagagcagag gagaatcgca tcagcgaacc ccgtaccaac gatcggatcc
     6181 gggtgccaga ggtacgcctc gtcgggcccg gcggtgaaca ggtcggcgtc gtgaagatcg
     6241 gcgtcgccct tcggctcgcg caagacgccg aactcgacct cgtcgaggtg gcgccgaact
     6301 cgaagccgcc ggtcgtcaag atcatggatt acggcaagta caagtatgag gccgcgcaga
     6361 aggcgaaaga ggcccgccgc aaccaagcca acacagtcct caaagaggtg cgcttccgcc
     6421 tcaagatcga cacgcacgat tacgagacca agcgcaagcg ggccgaaggg ttcctgcgct
     6481 ccggcgacaa ggtgaaggcg atgatcctct tccgcggtcg ggagcagtcc aggccggagc
     6541 tcggtgtgcg cctgttgcag cggttcgccg aggacgtcgg ggaatacggc tcggtcgagt
     6601 cgacaccgaa gatcgacggg cgcaacatgg tgatgatcgt cgggcctgtg aagaacaaag
     6661 ccgaggcccg tgccgaggcc aacgcgcaac gtggcgccac cagagaacgt caagtgccgc
     6721 gcaggaaccg tcctgcccgc gccgacggcc agcaaggctc gccggcccag gcgaccaacg
     6781 aggagaaatg atgccgaagc agaagaccaa ttccggggcg aagaaacggt tccggttgac
     6841 cggcagcggc aaggtgatga agcagcagtc cggtatgcgg cacaatctcg aggtcaaggc
     6901 gagccgccgt aagcgccgcc tgaaccaaga ccagctcgtg tccccggcgg acgcgaagaa
     6961 cgtcaagagg ctcttgggca agtgatccgc ccgcacgacg acgcagaaca cagagcctga
     7021 gcgaagagag agacacgaga gatggcaaga gtcaagaggg cggtaggcgc tcacaagaaa
     7081 cgtcgggtga ttctcgagcg cgccgagggg taccgcgggc tgcgctcgcg cctgtaccgc
     7141 aaggccaagg agcaggtcac ccattcgctc acctacagct accgggaccg ccgcgcacgt
     7201 aaaggcgact tccgccgcct ctggatccag cgcatcaacg ccgcgagccg ccaaaacggc
     7261 ctcacctaca accggttcat ccagggcctc ggcctggcgg gtgtcgaggt cgaccggaag
     7321 atcctggccg acctggcggt caacgacgcc cccgcgttcg cgg
//
LOCUS       DASTNL010000248         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacteriaceae bacterium isolate SMAG_U2141
            k141_926024, whole genome shotgun sequence.
ACCESSION   DASTNL010000248 DASTNL010000000
VERSION     DASTNL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960613
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacteriaceae bacterium (soil metagenome)
  ORGANISM  Microbacteriaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:55:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,625
            CDSs (total)                      :: 1,596
            Genes (coding)                    :: 1,589
            CDSs (with protein)               :: 1,589
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Microbacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926024"
                     /isolate="SMAG_U2141"
                     /isolation_source="soil"
                     /db_xref="taxon:1873462"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1562)
                     /locus_tag="VFQ96_08115"
     CDS             complement(111..1562)
                     /locus_tag="VFQ96_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0257788.1"
                     /translation="MDSADSAGRRQRAKLPSIGMVTVAVDNPVEADRVREMLAEEGSR
                     QDVRVIVVGEGGRGLPEGQPTIVAGSLVNALDFDDLAVPSRDFLRAPYTAKDLTMRIQ
                     VAAARSLHHVERFGPEDFARIIRQIADSLPTAIIFADAKGLPRLRNRAVRDVLTLAGY
                     DPATGMAEHVFGQDRKTRLKRGRNIVEDALAGRGRGVVYWIGDPAEPEKQRAIITVGH
                     TIRRPDGALLGTAMVIHDVTDTAVLIGERSDYLASLSHELRNPLTAAIGFLDLALEDP
                     DLHRQGSNIADQLATAQANLEHLHALLNRLSTIGLRSRSVTLSPTDLTVLMDRSIRAA
                     RRASADTGVRIVSHLRRKPMIGRADKVRLRQAVDNLISNAVKYTPEGGTVTVTLTRLD
                     DDALIHVADTGVGITRADQDKIFDRFYHTPSIRPAGAGGYGIGLAIARTVAHAHAGTL
                     TVESVPGQGSTFTLRIPLRPEGAPLPDIHDDTP"
     gene            2246..3418
                     /locus_tag="VFQ96_08120"
     CDS             2246..3418
                     /locus_tag="VFQ96_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEU0257789.1"
                     /translation="MLLNSVGTRTALSDAPLIPVPSTYNAPGVRHTAQIRAANWAGYV
                     ATGQKFTGVTGTWRVPSAVCTTADSFAAQWVGIDGHPSETVEQIGTGIACHDGVASYE
                     AWYEMYGDADVNGGASVTLPSGYKVTAGDTVTGTVTVAGDMWSLTLADTSRGWSFTKN
                     VTFHAEQASAEWIVERPDVCTSAEESSCQASTLTASKPVTFTAASAQAGGAMQPAATF
                     APAAATMVDVDSDATLASPSSLASTSFVVTIAGPDGVVVPASSQAPSCSSAPFTDVSK
                     TNAFCGAISWLKTSGITTGYAGGAFRPGASISRAAVAAFLYRLAHDGAQAPSCSSAPF
                     TDVSKTNAFCGAISWLKTSGITTGYAGGAFHPGASISRAAVAAFLYRLVALAGQAN"
     gene            complement(3505..>4179)
                     /locus_tag="VFQ96_08125"
     CDS             complement(3505..>4179)
                     /locus_tag="VFQ96_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU0257790.1"
                     /translation="FMMPYKFGIDEVNTKIQILREEFNQLHAYNPIEHLSSRLKTPES
                     IVEKVTRRGIEPSFDAIRETITDIAGVRVTCSFVSDVYRVFDLLTGQSDLKLIVAKDY
                     IANPKPNGYKSLHAIVAVPVFLSNGPFPVPVEIQLRTIAMDFWASLEHKIYYKYDHVV
                     PQDLLDGLKRAADTAAELDETMERLHAEIRGTGAPERPVAPAHFVPTDTSLLRIHQAM
                     HGPTEV"
BASE COUNT          575 a         1384 c         1496 g          724 t
ORIGIN      
        1 tgcgcccacc tggaggagct gggccatatc ggcccgctga tcgtcgcgct caacagcgat
       61 ctgaccgcgc agtgacctct cctttgcgta taccgctcga ccggcggcgg tcagggtgtg
      121 tcgtcgtgga tgtcgggcaa gggcgcgccc tcggggcgta gcggtatccg cagcgtgaac
      181 gtgctgcctt gcccgggtac gctctcgacg gtgagggtgc cggcgtgggc gtgggccacg
      241 gtgcgggcga tggccaggcc gattccgtag ccgccggccc cggccggccg gatgctgggc
      301 gtgtggtaga accggtcgaa gatcttgtct tggtcggccc gggtgatgcc gaccccggtg
      361 tcggcgacgt gaatgagcgc gtcgtcgtcg agccgggtga gcgtgaccgt gaccgtgccg
      421 ccctccggag tgtatttgac cgcgttcgag atgaggttgt cgaccgcctg gcgaagccgc
      481 accttgtcgg ccctgccgat catgggcttg cgtcgaaggt gagagacgat gcgcaccccc
      541 gtgtcggcgc tcgcccgccg cgcggcccgg atcgagcggt ccatgagaac ggtcaggtcg
      601 gtcggcgaga gggtcacgga gcgggatcgc aggccgatcg tcgacaggcg gttgagcagg
      661 gcgtggagat gctcgaggtt tgcctgcgcg gtcgccagct ggtcggcgat gttgctcccc
      721 tggcggtgca ggtcggggtc ttccagcgcg agatcgagga acccgatcgc ggcggtgagg
      781 gggttgcgca actcgtgcga gagcgaggcc aggtagtcgg agcgctctcc tatcaagacg
      841 gccgtgtccg tgacgtcgtg gatcaccatg gcggtgccga gcagcgctcc gtcggggcgg
      901 cggatggtgt gcccgaccgt gatgatcgcc ctctgcttct cgggctcggc cgggtcgccg
      961 atccagtaga cgacgccgcg cccgcggccc gccagggcgt cttcgacgat gttccggccg
     1021 cgtttcaacc gggtcttccg gtcttggccg aagacgtgct cggccatccc cgtcgccggg
     1081 tcgtagccgg ccaaggtcag gacgtcgcgc accgctcggt tgcgcagcct cggcaggccc
     1141 ttcgcatccg cgaagatgat ggcggtcgga aggctgtcgg cgatctggcg gatgatgcgc
     1201 gcgaagtcct ccggcccgaa ccgctccacg tggtgcaggc tgcgtgccgc cgccacctgg
     1261 atgcgcatgg tgaggtcttt cgccgtgtaa ggcgcccgca ggaagtcccg gctgggcacg
     1321 gcgaggtcgt cgaagtcgag ggcgttgacg agagaacccg cgacgatggt cggctgcccc
     1381 tccggcagcc ctcgaccgcc ctcgccgacg acgatgaccc gcacgtcttg ccgcgagccc
     1441 tcctcggcca gcatttctcg aacccggtcg gcctcgaccg ggttgtcgac cgcgaccgtc
     1501 accatgccga tcgagggcag cttcgccctc tgccgtcggc ccgccgaatc ggctgagtcc
     1561 atgctgaccc ctccacgatg ccgtcgtgcc gtggccacag cccgacttgt tgctgacaat
     1621 atcgccctgt gacaggtgga gggtaagtgt ggcgcagcct cggccgggta cggcacggtc
     1681 ggtcgggccg ggccgggccg ggccgggccg tgctgcggcc agcattgggc tctgcacgcg
     1741 aaggaccggg cgacgagcgc ggtcagtgcg atgtgcggta tcggacaccg gtcggattcg
     1801 tgcgcgcgaa gggatgggcc ggggctcgca tgggtggtga tgcaggggag tttcatggtg
     1861 agaacccggg gtttggcgtg acgcgggggc cggccgaccc gatattctcc agagaggcgc
     1921 ccggcgggta tttccctcct atggcggggc atgggcggga agaggcgata tgaggctgct
     1981 gaacgtgggg cgcccttcga cgacgaggcg tacgaaggcc gggcgtgcgc tcgcggcgtt
     2041 cggcgccggc gcggccactc tcgcgttaat catcggcggg ttgacgggcc ctggggctcc
     2101 tgccggtgcg gctcctgccg gtgcggcgcc cgccccatcg gctcttgttt cgtcggccgc
     2161 cgcgtcctcg gccatcgcct catcgcctac cgtctcgtcg gccgacgctc ctgctgcctc
     2221 cgctgtgcag gagtcgccga cggccgtgct gctcaactcc gtcggcaccc gtacggcgct
     2281 ttccgacgcg ccgctcatcc cggtgccctc cacgtacaac gcgccgggtg tgcggcacac
     2341 ggcgcagatc cgggccgcga actgggccgg ctacgtcgcc accgggcaga agttcaccgg
     2401 cgtgaccggc acctggcgcg tgccgagtgc cgtctgtacc acggcggatt ccttcgccgc
     2461 gcaatgggtc ggcatcgacg ggcatccgag cgagaccgtc gagcagatcg gcaccggcat
     2521 cgcctgccac gacggcgtcg cgagctacga ggcctggtac gagatgtacg gcgacgccga
     2581 cgtcaacggc ggcgcctccg tcaccctgcc cagcggctac aaagtgactg ccggcgacac
     2641 cgtcacggga acggtcaccg tcgcgggcga catgtggagc ctcacgctcg ccgacaccag
     2701 ccgtgggtgg agcttcacga agaacgtgac gttccatgcc gagcaggcct cggccgaatg
     2761 gatcgtggag cggcccgacg tgtgcacgtc ggcggaggag tcatcgtgcc aggcgagcac
     2821 cctgaccgcc tcgaagccgg tcaccttcac cgccgcctcc gcacaggcgg gcggggccat
     2881 gcagccggcc gccaccttcg cgccggccgc agcgacgatg gtcgacgtcg actccgacgc
     2941 caccctcgcc agcccctctt cgttggcgag cacgtcgttc gtcgtcacca tcgcagggcc
     3001 cgacggcgtc gtcgtgccgg cgtcgtcgca ggcgccgtcg tgttcctcgg cgccgttcac
     3061 ggatgtgtcg aagacgaacg cgttctgtgg cgcgatctcg tggttgaaga cctcgggtat
     3121 cacgacgggg tatgcgggtg gcgcgttccg tccgggtgcg tcgatctccc gcgccgccgt
     3181 cgccgcgttc ctctaccgcc tcgcccacga cggtgcgcag gcgccgtcgt gttcctcggc
     3241 gccattcacg gatgtgtcga agacgaacgc gttctgtggc gcgatctcgt ggttgaagac
     3301 ctcgggtatc acgacggggt atgcgggtgg cgcgttccat ccgggtgcgt cgatctcccg
     3361 cgccgccgtc gccgcattcc tctaccgcct cgtcgccctc gccggacagg cgaattaggc
     3421 ctgtcagggc cgaccaggca cggtagtgag ccccgccggc agcaggcggg ccggacacgg
     3481 gaggcgccgc ggggggcgcg tgcctcagac ctcggtgggg ccgtgcatcg cctggtgtat
     3541 gcgcaagagt gacgtgtcgg tcggcacgaa atgcgccggc gcgacagggc gttccggcgc
     3601 cccggtgccg cggatctcgg cgtgcagtct ctccatcgtc tcgtcgagct ccgccgccgt
     3661 gtctgcggcg cgtttcaggc cgtcgagcag gtcttgcggc accacgtggt cgtacttgta
     3721 gtaaatcttg tgttccagac tcgcccagaa gtccatcgcg atcgtgcgca actggatctc
     3781 cacgggcacg gggaacggcc cattcgacag gaagacagga accgccacga tcgcatgcag
     3841 gctcttgtag ccgttcggct tcgggttggc gatgtagtcc ttggcgacga tgagcttcag
     3901 gtcgctctgc ccggtcaaga gatcgaagac gcggtacacg tccgatacga agctgcacgt
     3961 cacgcgcacg ccggcgatat cggtgatcgt ctcgcggatc gcgtcaaagg agggttcgat
     4021 gccgcgccgc gtgaccttct ccacgatgct ttccggggtc ttcaagcggc tcgagaggtg
     4081 ttcgatcggg ttgtaggcgt gcagctggtt gaactcttcc cgcaagatct ggatcttcgt
     4141 gttcacctcg tcgatgccga acttgtacgg catcatgaa
//
